From tajibade <@t> echd.org Mon Apr 2 10:29:56 2012 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Mon Apr 2 10:30:09 2012 Subject: [Histonet] IHC QC log from the Ref Lab Message-ID: Hello Everyone, If you send IHC to a ref lab to do the technical components only and you have pathologists onsite to interpret the slides, do you need the QC log for that stain from that ref lab? If you already have both positive and negative control slides from that ref lab. Thank you. Tunde Ajibade BS, HTL(ASCP)QIHC CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From etambutte <@t> centrescientifique.mc Mon Apr 2 12:02:29 2012 From: etambutte <@t> centrescientifique.mc (Eric Tambutte) Date: Mon Apr 2 12:02:39 2012 Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 1 Message-ID: <1082300494@s15272523.onlinehome-server.info> Bonjour, Je suis absent du laboratoire jusqu'au jeudi 05 avril 2012. Je vous répondrai le plus rapidement possible. Eric Tambutté Thank you for your mail. I will be out of office till April 05th 2012. I will respond to your e-mail as soon as possible. Thank you for your understanding. Best regards Eric Tambutté From VHammel <@t> primecare.org Mon Apr 2 14:31:13 2012 From: VHammel <@t> primecare.org (Hammel, Vicky) Date: Mon Apr 2 14:32:30 2012 Subject: [Histonet] FW: In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F76833A2C6938B5F@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F76833A2C6938B5F@EXCHANGE2K7.staprimecare.org> Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2C6938B60@EXCHANGE2K7.staprimecare.org> ________________________________ I am currently working up two new antibodies: Hepatocyte Specific Antigen and Glypican-3. I am looking to find a block of yolk sac tumor - either testis or ovary and a block of Hepatacullar carcinoma. If anyone has blocks, I have melanoma or H. pylori I could trade, please contact me. Thank you, Vicky Hammel HTL, ASCP Pathology Technical Consultant vhammel@primecare.org St. Alexius Medical Center Histology Laboratory 900 east Broadway Bismarck, ND 58506 ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From jclark <@t> pcnm.com Mon Apr 2 14:35:39 2012 From: jclark <@t> pcnm.com (Joanne Clark) Date: Mon Apr 2 14:35:53 2012 Subject: [Histonet] RE: Histonet Digest, Vol 101, Issue 1 In-Reply-To: <20120402170335.4D8BD8B7108@mx10.myoutlookonline.com> References: <20120402170335.4D8BD8B7108@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E3784CB76A68@S10MAILD001N1.SH10.lan> We don't. And CAP had never questioned it. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 Apr 2012 10:29:56 -0500 From: Tunde Ajibade Subject: [Histonet] IHC QC log from the Ref Lab To: "'histonet-bounces@lists.utsouthwestern.edu'" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Everyone, If you send IHC to a ref lab to do the technical components only and you have pathologists onsite to interpret the slides, do you need the QC log for that stain from that ref lab? If you already have both positive and negative control slides from that ref lab. Thank you. Tunde Ajibade BS, HTL(ASCP)QIHC CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 101, Issue 1 **************************************** From JMacDonald <@t> mtsac.edu Mon Apr 2 16:45:25 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Apr 2 16:46:21 2012 Subject: [Histonet] Leica Rep Message-ID: Does anyone know the Leica Rep for Southern California? Thanks, Jennifer From khairedai <@t> yahoo.com Mon Apr 2 23:30:35 2012 From: khairedai <@t> yahoo.com (Khaire Dai) Date: Mon Apr 2 23:31:39 2012 Subject: [Histonet] (no subject) Message-ID: <1333427435.60377.YahooMailMobile@web113014.mail.gq1.yahoo.com> http://sandbox.spedman975.net/refugee.old/wp-content/plugins/extended-comment-options/02efpk.html From paperboats11 <@t> gmail.com Tue Apr 3 07:15:31 2012 From: paperboats11 <@t> gmail.com (Diana Matos) Date: Tue Apr 3 07:15:39 2012 Subject: [Histonet] Cell block - Shandon kit - help? Message-ID: Hello everyone! In our lab, we've been trying out the Shandon CB kit and we've been having a few problems. We use non-buffered 10% formalin as a fixative (overnight), follow the manual's instructions in regards to ratio of reagents/size of pellet and follow a regular 10h histological processing protocol. But when it comes to embedding, the little thin membrane-like cell buttons are slightly too buoyant than supposed and there's some retraction afterwards, even if we leave the blocks to be cut the next day. Possibly bad paraffin impregnation?The best results have been obtained with smaller pellets but since we need to adapt this protocol to all sorts of samples, we need to optimize it. Have any of you tried this out? What have you tweaked within the regular protocol? Any tips? Any input would be welcome! Thank you! Diana Portugal From fourfonners <@t> yahoo.com Tue Apr 3 08:51:12 2012 From: fourfonners <@t> yahoo.com (Sheila Fonner) Date: Tue Apr 3 08:51:17 2012 Subject: [Histonet] H & E Staining Message-ID: <1333461072.65199.YahooMailNeo@web31910.mail.mud.yahoo.com> Good morning Histonetters, ? Can anyone?tell me if you are using a particular company's reagents for your H&E staining that are pre-made and meant to work together?? Also, is the cost significantly more than buying separate reagents and making up your own? ? Thanks, Sheila, HT (ASCP) Knoxville, TN From BDeBrosse-Serra <@t> isisph.com Tue Apr 3 09:05:00 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Apr 3 09:05:16 2012 Subject: [Histonet] H & E Staining In-Reply-To: <1333461072.65199.YahooMailNeo@web31910.mail.mud.yahoo.com> References: <1333461072.65199.YahooMailNeo@web31910.mail.mud.yahoo.com> Message-ID: <493CAA64F203E14E8823737B9EE0E25F0921436460@EXCHMB01.isis.local> Sheila, We are using the reagents from Leica, formerly Surgipath, Gill's II Hematoxylin (01520) and Eosin (01520) on our Leica Autostainer. But there are many other vendors that are very good too. Way back when, we used to make our own hematoxylin and eosin. But it really is so much easier to buy it ready made. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2588 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, April 03, 2012 6:51 AM To: Histonet Subject: [Histonet] H & E Staining Good morning Histonetters, ? Can anyone?tell me if you are using a particular company's reagents for your H&E staining that are pre-made and meant to work together?? Also, is the cost significantly more than buying separate reagents and making up your own? ? Thanks, Sheila, HT (ASCP) Knoxville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micropathlabs <@t> yahoo.com Tue Apr 3 09:17:07 2012 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Tue Apr 3 09:17:14 2012 Subject: [Histonet] H & E Staining In-Reply-To: <493CAA64F203E14E8823737B9EE0E25F0921436460@EXCHMB01.isis.local> References: <1333461072.65199.YahooMailNeo@web31910.mail.mud.yahoo.com> <493CAA64F203E14E8823737B9EE0E25F0921436460@EXCHMB01.isis.local> Message-ID: <1333462627.20538.YahooMailNeo@web161704.mail.bf1.yahoo.com> Not only is it easier, it's more consistent. We use Richard-Allen Hematoxylin II and Eosin. They stain beautifully. There are many different vendors, you'll have to find one your pathologists like. Hope this helps. Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. From: Bea DeBrosse-Serra To: 'Sheila Fonner' ; Histonet Sent: Tuesday, April 3, 2012 10:05 AM Subject: RE: [Histonet] H & E Staining Sheila, We are using the reagents from Leica, formerly Surgipath, Gill's II Hematoxylin (01520) and Eosin (01520) on our Leica Autostainer. But there are many other vendors that are very good too. Way back when, we used to make our own hematoxylin and eosin. But it really is so much easier to buy it ready made. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2588 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, April 03, 2012 6:51 AM To: Histonet Subject: [Histonet] H & E Staining Good morning Histonetters, ? Can anyone?tell me if you are using a particular company's reagents for your H&E staining that are pre-made and meant to work together?? Also, is the cost significantly more than buying separate reagents and making up your own? ? Thanks, Sheila, HT (ASCP) Knoxville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Apr 3 09:30:57 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 3 09:31:05 2012 Subject: [Histonet] H & E Staining In-Reply-To: <1333461072.65199.YahooMailNeo@web31910.mail.mud.yahoo.com> Message-ID: <1333463457.5465.YahooMailClassic@web162104.mail.bf1.yahoo.com> I always used Richard Allan (Hematox; clarifier, bueing) and was always satisfied. Ren? J. --- On Tue, 4/3/12, Sheila Fonner wrote: From: Sheila Fonner Subject: [Histonet] H & E Staining To: "Histonet" Date: Tuesday, April 3, 2012, 9:51 AM Good morning Histonetters, ? Can anyone?tell me if you are using a particular company's reagents for your H&E staining that are pre-made and meant to work together?? Also, is the cost significantly more than buying separate reagents and making up your own? ? Thanks, Sheila, HT (ASCP) Knoxville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmcgough <@t> clinlab.com Tue Apr 3 09:45:39 2012 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Tue Apr 3 09:45:47 2012 Subject: [Histonet] Dako Pharm DX Kit for ER/PR In-Reply-To: <2B6E973D7D12964F8D8A3598557C553004ED21@NSH-SRVR01.nsh.local> Message-ID: We are wondering how many labs are using Dako's PharmDX kit for their ER/PR's. Do you find it reliable? Do you have many repeats? Thank you for your responses. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Apr 3 09:48:44 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Apr 3 09:50:07 2012 Subject: [Histonet] Dako Pharm DX Kit for ER/PR In-Reply-To: References: <2B6E973D7D12964F8D8A3598557C553004ED21@NSH-SRVR01.nsh.local>, Message-ID: We use it and find it very reliable. We have also found that the control tissue can lose its antigenicty if cut too far in advance and allowed to sit, mostly for the ER. Haven't seen this drop off with the PR. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason McGough [jmcgough@clinlab.com] Sent: Tuesday, April 03, 2012 10:45 AM To: Histonet@lists.utsouthwestern.edu Cc: Scott Johnson; Janna Hope Subject: [Histonet] Dako Pharm DX Kit for ER/PR We are wondering how many labs are using Dako's PharmDX kit for their ER/PR's. Do you find it reliable? Do you have many repeats? Thank you for your responses. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> georgetownhospitalsystem.org Tue Apr 3 10:48:23 2012 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Tue Apr 3 10:48:33 2012 Subject: [Histonet] AFB by IHC Message-ID: My pathologist wants an AFB stain done by IHC. Is this stain/procedure even doable and if so where? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From Kay.Nabers <@t> trumbulllabs.com Tue Apr 3 11:13:27 2012 From: Kay.Nabers <@t> trumbulllabs.com (Kay W. Nabers) Date: Tue Apr 3 11:13:36 2012 Subject: [Histonet] GI biopsies Message-ID: Is any one timing fixation for GI biopsies like breast cores for GISTs to do IHC on ? If so , how long? Thanks. From rjbuesa <@t> yahoo.com Tue Apr 3 11:45:16 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 3 11:45:21 2012 Subject: [Histonet] AFB by IHC In-Reply-To: Message-ID: <1333471516.12904.YahooMailClassic@web162102.mail.bf1.yahoo.com> Amy: I think you should clarify with your pathologist this request because AFB, as you perfectly know,?is a procedure in itself. Is it that s/he wants to detect acid fast bacteria with an IHC method instead of the Ziehl-Nielsen method? In that case your only option is to look in the IHC providers catalogs if an antibody has been developed for? the different types of Mycobacteria and use it. Perhaps your pathologist has found out that H. pyloris are now detected in many labs using IHC and s/he wants to do the same with Mycobacteria. You will need to get a clarification about that request. Ren? J. --- On Tue, 4/3/12, Amy Self wrote: From: Amy Self Subject: [Histonet] AFB by IHC To: "'histonet@lists.utsouthwestern.edu'" Date: Tuesday, April 3, 2012, 11:48 AM My pathologist wants an AFB stain done by IHC.? Is this stain/procedure even doable and if so where? Thanks in advance for your help,? Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Apr 3 11:49:57 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 3 11:50:01 2012 Subject: [Histonet] GI biopsies In-Reply-To: Message-ID: <1333471797.50549.YahooMailClassic@web162103.mail.bf1.yahoo.com> Not that I know, but beware: not because the GI biopsies are small it means that they can be fixed for less time. The fixation mechanism with formalin (penetration ? binding ? cross linking = fixation) is independent of the size of the sample. It is a time related?event dependent on the chemistry of the reaction and the temperature, but independent of the amount of formalin or the size of the tissue. Ren? j. --- On Tue, 4/3/12, Kay W. Nabers wrote: From: Kay W. Nabers Subject: [Histonet] GI biopsies To: "Histonet@lists.utsouthwestern.edu" Date: Tuesday, April 3, 2012, 12:13 PM Is any one timing fixation for GI biopsies like? breast cores for GISTs to do IHC on ? If so , how long? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kay.Nabers <@t> trumbulllabs.com Tue Apr 3 11:56:22 2012 From: Kay.Nabers <@t> trumbulllabs.com (Kay W. Nabers) Date: Tue Apr 3 11:56:27 2012 Subject: [Histonet] gi cancers Message-ID: Also meant to add GI cancers on biopsies for Her2 for fixing times to my earlier inquiry.Thanks, again. From TGoins <@t> mt.gov Tue Apr 3 12:07:54 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Tue Apr 3 12:08:05 2012 Subject: [Histonet] H & E Staining In-Reply-To: <1333461072.65199.YahooMailNeo@web31910.mail.mud.yahoo.com> References: <1333461072.65199.YahooMailNeo@web31910.mail.mud.yahoo.com> Message-ID: We use Platinum Line hematoxylin and eosin from Mercedes Medical [MER4799 and MER4131] - much cheaper than Richard-Allan and in a side-by-side blind test, the pathologists actually preferred Platinum Line. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, April 03, 2012 7:51 AM To: Histonet Subject: [Histonet] H & E Staining Good morning Histonetters, ? Can anyone?tell me if you are using a particular company's reagents for your H&E staining that are pre-made and meant to work together?? Also, is the cost significantly more than buying separate reagents and making up your own? ? Thanks, Sheila, HT (ASCP) Knoxville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Apr 3 12:14:53 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Apr 3 12:16:23 2012 Subject: [Histonet] RE: GI biopsies In-Reply-To: References: Message-ID: We are moving to timing everything. From ishcemic time (time out of patient to time in formalin). And also the length of time in fixation. More so that we can track something back if there is a problem anywhere along the line. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kay W. Nabers [Kay.Nabers@trumbulllabs.com] Sent: Tuesday, April 03, 2012 12:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] GI biopsies Is any one timing fixation for GI biopsies like breast cores for GISTs to do IHC on ? If so , how long? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Apr 3 12:20:09 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Apr 3 12:21:05 2012 Subject: [Histonet] RE: GI biopsies In-Reply-To: References: , Message-ID: sorry typo "ischemic" not ishcemic.... Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A [Loralee_Mcmahon@URMC.Rochester.edu] Sent: Tuesday, April 03, 2012 1:14 PM To: Kay W. Nabers; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: GI biopsies We are moving to timing everything. From ishcemic time (time out of patient to time in formalin). And also the length of time in fixation. More so that we can track something back if there is a problem anywhere along the line. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kay W. Nabers [Kay.Nabers@trumbulllabs.com] Sent: Tuesday, April 03, 2012 12:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] GI biopsies Is any one timing fixation for GI biopsies like breast cores for GISTs to do IHC on ? If so , how long? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa <@t> alliedsearchpartners.com Tue Apr 3 14:10:08 2012 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Tue Apr 3 14:10:20 2012 Subject: [Histonet] IHC and Cytotechnologist Job Openings in New York Message-ID: Allied Search Partners has been retained for the following searches. We have openings in Immunohistochemistry and Cytotechnology. Please forward this along to anyone who you know that would be interested in any of the following positions. We do offer generous referral bonuses. 1. Please email a copy of updated resume to melissa@alliedsearchpartners.com or fax to 888-388-7572 for a full job description. We have the following positions available: Immunohistochemistry (IHC) Histotech LOCATION: East White Plains, NY area DEPARTMENT & SCHEDULE: Monday-Friday Overnight 3rd Shift Hours Cytotechnologist LOCATION: Hicksville, NY area SCHEDULE & DEPARTMENT Monday-Friday Day Shift Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com From shive003 <@t> umn.edu Tue Apr 3 14:10:34 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Apr 3 14:12:36 2012 Subject: [Histonet] turkey reovirus Message-ID: Does anyone know of an antibody for Reovirus that can be used successfully with FFPE tissue? I have not been able to find one online yet that can be used with IHC application. Thanks in advance, Jan Shivers IHC/Histo/EM Vet. Diag. Lab Univ. of Minnesota From BJANICEK2 <@t> unl.edu Tue Apr 3 14:27:19 2012 From: BJANICEK2 <@t> unl.edu (Brandy Janicek) Date: Tue Apr 3 14:27:35 2012 Subject: [Histonet] Cassette Printer Message-ID: <926B32A680D22D47AEC1A900C3FA0C47B9BAB5@SN2PRD0802MB098.namprd08.prod.outlook.com> I am looking for suggestions for a new cassette printer that can print in batches and has multiple magazines for different cassette colors. We currently have a Leica IP-C that we have had issues with. Has anyone used the cassette printer from Mercedes Medical? Suggestions and reviews would be much appreciated! Thank you! Brandy N. Janicek Histology Manager UNL-Veterinary Diagnostic Center Fair Street & East Campus Loop Lincoln, NE 68583 402-472-8474 From sllandsc <@t> gmail.com Tue Apr 3 14:33:35 2012 From: sllandsc <@t> gmail.com (Sara Landschoot) Date: Tue Apr 3 14:33:39 2012 Subject: [Histonet] Masson's Trichrome for Bone Message-ID: Hi! I work at an Orthopaedic research lab and I have been having some trouble getting our trichromes to work on our bone. I have tried a masson's, gomori, and goldner's and in all cases the bone stained red. Our bone is arrive to our lab frozen, we fix them in formalin, embed in paraffin, and decal with EDTA. I also post fix with Bouins before staining. Techs that worked in this lab before me were also getting the same red staining of bone. Is there something during processing that could cause this reaction? We fix in formalin, dehydrate through graded alcohols, and clear in xylene. Thanks, Sara From gagnone <@t> KGH.KARI.NET Tue Apr 3 14:51:40 2012 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Tue Apr 3 14:51:54 2012 Subject: [Histonet] HIS-Copath-Paperless Order Entry Message-ID: <5F06C3AD0B27264CA20CFA986C87882EAF0F@EXCHANGEPV1.KGH.ON.CA> Have any histonetters had experience with paperless order entry using a HIS-Copath interface? All our clinical laboratories have just gone to paperless order entry on patient floors and clinics, and would be interested in any other sites' experiences that use these same systems. We have already found that pathology order entry is quite different from other clinical laboratories that now receive pre-barcoded tubes of blood that are instrument-ready. Pathology has not been quite that straightforward. Thanks for any experiences or advice you wish to share, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From rsrichmond <@t> gmail.com Tue Apr 3 14:54:14 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Apr 3 14:54:19 2012 Subject: [Histonet] Re: AFB by IHC Message-ID: Amy Self asks: >>My pathologist wants an AFB stain done by IHC. Is this stain/procedure even doable and if so where?<< AFB by polymerase chain reaction (PCR) is what he may be thinking of. PCR can be done on paraffin blocks, but not every reference lab does - I think Mayo Medical Laboratories may. Your pathologist might be better advised to do a fluorescent AFB stain (Auramine O), if your pathologist has access to a fluorescence microscope. This is a much better technique than the light microscopic AFB stain, but though it's been around for half a century it's very little used. I think the regulatory agencies should ban light microscopic AFB stains, because of their insensitivity. Bob Richmond Samurai Pathologist Knoxville TN From Rcartun <@t> harthosp.org Tue Apr 3 15:45:09 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Apr 3 15:45:18 2012 Subject: [Histonet] AFB by IHC In-Reply-To: References: Message-ID: <4F7B2914.7400.0077.1@harthosp.org> Yes, you can do IHC for mycobacteria; however, there are issues with specificity depending on the primary antibody that you use. I have been using a polyclonal antibody from Dako (no longer available) for many years and although it is very sensitive for identifying mycobacteria, it does cross-react with other bacteria. I have not found a monoclonal antibody (yet) that works on formalin-fixed, paraffin-embedded tissue. In addition, your IHC assay has to be exquisitely clean in order to trust the rare immunoreactivity that may be present. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Amy Self 4/3/2012 11:48 AM >>> My pathologist wants an AFB stain done by IHC. Is this stain/procedure even doable and if so where? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Tue Apr 3 17:07:18 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Apr 3 17:07:30 2012 Subject: [Histonet] HIS-Copath-Paperless Order Entry In-Reply-To: <5F06C3AD0B27264CA20CFA986C87882EAF0F@EXCHANGEPV1.KGH.ON.CA> References: <5F06C3AD0B27264CA20CFA986C87882EAF0F@EXCHANGEPV1.KGH.ON.CA> Message-ID: I've used it. We used it to track all our off site specimens. It was great for catching that one specimen that someone would leave in the wrong place very rarely. Yes, it meant we had to do a little extra work and sometimes we had to go on hunts for the specimen we knew was out there but that beat the alternative. The only complaint we ever had other than extra work was ours wouldn't remove from the order list multiple specimens from the same case. We had to remove those manually. Its a good tool for tracking though. Kim D Sent from my iPhone On Apr 3, 2012, at 3:51 PM, "Gagnon, Eric" wrote: > Have any histonetters had experience with paperless order entry using a HIS-Copath interface? All our clinical laboratories have just gone to paperless order entry on patient floors and clinics, and would be interested in any other sites' experiences that use these same systems. > > We have already found that pathology order entry is quite different from other clinical laboratories that now receive pre-barcoded tubes of blood that are instrument-ready. Pathology has not been quite that straightforward. > > Thanks for any experiences or advice you wish to share, > > Eric Gagnon MLT > Histology Laboratory > Kingston General Hospital > Kingston, Ontario, Canada > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From khairedai <@t> yahoo.com Tue Apr 3 20:10:35 2012 From: khairedai <@t> yahoo.com (Khaire Dai) Date: Tue Apr 3 20:10:41 2012 Subject: [Histonet] (no subject) Message-ID: <1333501835.37326.YahooMailMobile@web113003.mail.gq1.yahoo.com> http://themarilynmonroe.info/wp-includes/theme-compat/jrklre.html From gu.lang <@t> gmx.at Wed Apr 4 02:31:15 2012 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Apr 4 02:31:22 2012 Subject: AW: [Histonet] Masson's Trichrome for Bone In-Reply-To: References: Message-ID: <003501cd1234$eb4aa730$c1dff590$@gmx.at> Perhaps I am wrong. But doesn't the trichrome differentiate between mature and fresh bone tissue? Old one - red; new one - blue (or green) Depending on the amount of collagen. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sara Landschoot Gesendet: Dienstag, 03. April 2012 21:34 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Masson's Trichrome for Bone Hi! I work at an Orthopaedic research lab and I have been having some trouble getting our trichromes to work on our bone. I have tried a masson's, gomori, and goldner's and in all cases the bone stained red. Our bone is arrive to our lab frozen, we fix them in formalin, embed in paraffin, and decal with EDTA. I also post fix with Bouins before staining. Techs that worked in this lab before me were also getting the same red staining of bone. Is there something during processing that could cause this reaction? We fix in formalin, dehydrate through graded alcohols, and clear in xylene. Thanks, Sara _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Wed Apr 4 11:50:11 2012 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Wed Apr 4 11:50:15 2012 Subject: [Histonet] Slippery Floor due to paraffin Message-ID: Hello to all in histoland. What are histology labs doing to combat the slipperiness of the floor due to paraffin. Are you using rugs, peel away films ? Any help would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From LSebree <@t> uwhealth.org Wed Apr 4 12:21:24 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Apr 4 12:21:31 2012 Subject: [Histonet] Slippery Floor due to paraffin In-Reply-To: References: Message-ID: Peel off film here...works well. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, April 04, 2012 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slippery Floor due to paraffin Hello to all in histoland. What are histology labs doing to combat the slipperiness of the floor due to paraffin. Are you using rugs, peel away films ? Any help would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mw <@t> personifysearch.com Wed Apr 4 12:31:10 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed Apr 4 12:31:19 2012 Subject: [Histonet] New Position Alert - IHC Field Tech Atlanta Message-ID: <33b696097ef326f96a51a0f52df63358@mail.gmail.com> Good Afternoon Histonet, We have had a global leader in IHC and Histology open a field based IHC support opportunity covering the Southeast Region. The ideal location would be to be based in Atlanta and the position is open due to promotion. If you or anyone you may know has a strong background in IHC and is looking to break out of the lab into a field role please contact me directly to learn more. The position offers a Base Salary + Bonus + Full Benefits (Car Allowance, Corporate Credit Card, Cell Phone, Laptop, Home Office, Full Health, 401k). Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From oneilb <@t> wvuhealthcare.com Wed Apr 4 13:08:14 2012 From: oneilb <@t> wvuhealthcare.com (Oneil, Beth Ann) Date: Wed Apr 4 13:08:27 2012 Subject: [Histonet] Glassware washing Message-ID: <3CEB8EBCF9C7A648B9694B5696462A71EFE0@NT-EXMB2.wvuh.wvuhs.com> Our laboratory does about half of it's daily special stains manually which means we go through a lot of Copeland jars. We currently wash everything by hand using liquinox with a splash of bleach in the water. We rinse with tap water. My question is how do other labs address the CAP issue of testing their glassware for detergent removal. When I worked in the chemistry lab, we didn't go through a lot of glassware so we were able to take a monthly bin of dirty glassware to sterile processing for cleaning, and then just check one representative piece of glassware from the batch for detergent. I can't figure out how my lab can check our glassware when there is so much, we wash it all throughout the day. Thank you for any help. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor/Technical Specialist West Virginia University Hospitals 304-293-7629 (office) 304-293-6014 (lab) From gayle.callis <@t> bresnan.net Wed Apr 4 13:13:26 2012 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Apr 4 13:13:59 2012 Subject: [Histonet] Re: Massons Trichrome on decalcified bone Message-ID: <000a01cd128e$a30c7110$e9255330$@bresnan.net> Dear Sara, You wrote: I work at an Orthopaedic research lab and I have been having some trouble getting our trichromes to work on our bone. I have tried a masson's, gomori, and goldner's and in all cases the bone stained red. Our bone is arrive to our lab frozen, we fix them in formalin, embed in paraffin, and decal with EDTA. I also post fix with Bouins before staining. Techs that worked in this lab before me were also getting the same red staining of bone. Is there something during processing that could cause this reaction? We fix in formalin, dehydrate through graded alcohols, and clear in xylene. **************************************************************************** **************************** I doubt your processing has any effect on Mass Tri staining. However, incomplete decalcification can but I suspect it may be the staining protocol itself. Using a simple weight loss/weight gain decalcification end point test with EDTA is a good idea, and can be used for acid decalcification endpoint testing IF you are not presently using an endpoint test. Years ago, I visited the AFIP bone lab, and acquired a Massons Trichrome protocol which worked much better than the standard Massons Trichrome found in most textbooks. It never failed to work well with our decalcified bone work although we did use acid decalcification. This is NOT a kit method. All reagents are made up in house, and post mordant heating Bouins is NOT done in a microwave. We preferred to let the sections sit in Boiuns overnight at RT, or heat in a water bath. We never used a dry heat, incubator type oven where one gets uneven heating in the chamber. There is more to doing Massons Trichrome on decalcified bone other than just following the recipe from a textbook. One of the best discussions on understanding the chemistry/theory of trichrome staining is found in Sheehan and Hrapchak, Theory and Practice of Histotechnology. The AFIP method has some different staining times, plus how to remove the biebrich scarlet/acid fuchsin from collagen BEFORE going into aniline blue, a much more controlled staining method. It also has a modified Weigerts Iron hematoxylin that is superior, a bit more concentrated since Iron hematoxylin tends to be removed by the acidic staining solutions. I will be happy to send these methods to you privately. Gayle M. Callis HTL/HT/MT(ASCP) From Maxim_71 <@t> mail.ru Wed Apr 4 15:20:15 2012 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Wed Apr 4 14:19:52 2012 Subject: [Histonet] Automatic knife sharpener Message-ID: <1182039392.20120404232015@mail.ru> Dear Histonetters! Can anybody to advise, what type automatic knife sharpener we can buy in Europe (Germany) instead Leica SP9000, which now is unavailable for sale? What vendors? We do not like a Shandon "Autosharp 5" type sharpener. Maxim Peshkov Russia, Taganrog. mailto:Maxim_71@mail.ru From amber.mckenzie <@t> gastrodocs.net Wed Apr 4 14:26:06 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Apr 4 14:24:10 2012 Subject: [Histonet] Grossing rules In-Reply-To: <3CEB8EBCF9C7A648B9694B5696462A71EFE0@NT-EXMB2.wvuh.wvuhs.com> References: <3CEB8EBCF9C7A648B9694B5696462A71EFE0@NT-EXMB2.wvuh.wvuhs.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BC11631D48@JERRY.Gia.com> Are there any guidelines on how to do GI grossing? At what size to you bisect? Do you call 5 or more pieces multiple or do you count all the pieces? How many categories are there: 1, 2, 3, several, multiple, etc...At what size is it considered a fragment and what size is a polyp? Thanks! From Timothy.Morken <@t> ucsfmedctr.org Wed Apr 4 14:26:19 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Apr 4 14:26:35 2012 Subject: [Histonet] EM tissue processors Q's Message-ID: <8D7C2D242DBD45498006B21122072BF8A4DDF412@MCINFRWEM003.ucsfmedicalcenter.org> Hi, we're looking around at different automated TEM tissue processors. We have an RMC 5160 but it has had a lot of problems so we are considering something else. I've looked online at Leica TP and Lynx II for routine processing. We may get to MW processing later, but the price is higher than authorized right now. Any suggestions or comments? Thanks for any info! Tim Morken Supervisor, Electron Microscopy Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94115 (415) 353-2673 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org From Allison_Scott <@t> hchd.tmc.edu Wed Apr 4 14:57:46 2012 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Wed Apr 4 14:57:51 2012 Subject: [Histonet] Specimen Identity Procedure Message-ID: Hello again to all in histoland. Does anyone have a procedure for describing the system for maintaining the identity of every specimen through processing and block and slide preparation. This is on the CAP checklist (ANP.21050). Any help will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From shive003 <@t> umn.edu Wed Apr 4 15:05:50 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Apr 4 15:05:54 2012 Subject: [Histonet] EM tissue processors Q's In-Reply-To: <8D7C2D242DBD45498006B21122072BF8A4DDF412@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF8A4DDF412@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: We use the Leica TP for our samples and endorse it. Jan Shivers IHC/Histo/EM Section Head Veterinary Diagnostic Lab UMN College of Veterinary Medicine St. Paul, MN On Wed, Apr 4, 2012 at 2:26 PM, Morken, Timothy < Timothy.Morken@ucsfmedctr.org> wrote: > Hi, we're looking around at different automated TEM tissue processors. We > have an RMC 5160 but it has had a lot of problems so we are considering > something else. I've looked online at Leica TP and Lynx II for routine > processing. We may get to MW processing later, but the price is higher than > authorized right now. > > Any suggestions or comments? > > Thanks for any info! > > > Tim Morken > Supervisor, Electron Microscopy > Department of Pathology > UC San Francisco Medical Center > 505 Parnassus Ave, Box 1656 > Room S570 > San Francisco, CA 94115 > > (415) 353-2673 (ph) > (415) 514-3403 (fax) > tim.morken@ucsfmedctr.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Histolaw69 <@t> aol.com Wed Apr 4 16:03:44 2012 From: Histolaw69 <@t> aol.com (Lawrence Allen) Date: Wed Apr 4 16:03:57 2012 Subject: [Histonet] Bone (Trichrome) Message-ID: Why not sent in 10 % formalin. Freezing it may change the morphology of the bone to fixation after thawing. I would give that a try. After all, you are running 3 different stains at one time in order. Lawrence S Allen Lead Histotechnologist Dorm VA Medical Center Columbia, SC From POWELL_SA <@t> mercer.edu Wed Apr 4 16:54:42 2012 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Apr 4 16:54:52 2012 Subject: [Histonet] Region III final reminder Message-ID: <9BF995BC0E47744E9673A41486E24EE24B330F1C3D@MERCERMAIL.MercerU.local> Hi Histotechs, This email is to remind you of the Georgia Society for Histotechnology hosting Region III meeting at Callaway Gardens, Pine Mountain GA April 13-15th,. This is next week friends. We will NOT be in the conference center as last year, and that is a GOOD thing. We will be in the ballroom area where we will have more space. We have far exceeded our original block of rooms, extended the deadline and now if the Mountain Creek Inn has any available, they will allow the GSH room rate. Please use the GSH Group # # 78K711 to get the discounted room rate of $109. Make reservations now at 1-800-225-5292. If you have not registered for the meeting please do so. PLEASE REGISTER NOW AS "AWAITING FUNDS" if you are waiting approval for the meeting go ahead and register for your workshops, luncheon, dinner and note on the form that you are awaiting funds below the total line. When approved, then send the funds, or if time does not allow, pay on site. But please register as soon as possible, don't wait, workshops are filling up fast. We also will have Histotalk's Dave Kemler at the meeting to interview some of our attendees as well. Also make plans to attend the Carriage and Horses Dinner on Saturday night for great food, great friends and good entertainment. The deadline for reservations for the dinner is April 9th, that is next Monday. Please make sure you fill out your registration form completely and email the form to Anne Taylor, GSH Treasurer to confirm your workshops as awaiting funds and then pay at the registration desk on site since time is short. If you have questions or concerns please contact Mike Ayers at lmayers@charter.net, Wanda Simons at Wandrous@att.net or myself at powell_sa@mercer.edu. We are excited to have 29 vendors exhibiting at our meeting and more signing up as we approach the meeting. BioCare ~Sponsor of new IHC Award BioGenex B/R Instruments Cancer Diagnostics Cell Marque Choice Medical Clarient~ sponsoring dinner at Dagher's Dako EMS Epitomics General Data IMEB Lab Storage Leica Microsystems ~ sponsoring dinner at Dagher's Leica Biosystems Mopec PolyScientific R&D - sponsor of new award "TBA" Sakura - Sponsor of GSH Histotechnologist of the Year Award Stat Lab Southeast Pathology Thermo Fisher - TBS ScyTek StatLab Ventana Those Not attending but supporting GSH are: Anatech - Unmanned table CL Sturkey - Door prizes LABSCO - Sponsoring a break Newcomer - Sponsoring a Break and provided totes Come Experience ____________ Histotechnology - Southern Style From TWELLEN <@t> LHS.ORG Wed Apr 4 19:06:04 2012 From: TWELLEN <@t> LHS.ORG (Wellen, Terrence D. :LPH Lab) Date: Wed Apr 4 19:05:59 2012 Subject: [Histonet] Leica Bond IHC Platform Message-ID: <5F46C87AD649864F9FA8273E8E31507A1607E0@SWM2006.LHSNT.LEGACYHS> Does anyone have any experience with this product? Terrence Wellen HT(ASCP) Legacy Good Samaritan Hospital Portland, OR From khairedai <@t> yahoo.com Wed Apr 4 22:46:34 2012 From: khairedai <@t> yahoo.com (Khaire Dai) Date: Wed Apr 4 22:46:38 2012 Subject: [Histonet] (no subject) Message-ID: <1333597594.50037.YahooMailMobile@web113010.mail.gq1.yahoo.com> http://gearsnow.com/wp-content/plugins/extended-comment-options/fjgvkd.html From mervatawad <@t> aol.com Thu Apr 5 05:09:51 2012 From: mervatawad <@t> aol.com (mervatawad@aol.com) Date: Thu Apr 5 05:10:01 2012 Subject: [Histonet] please don't sent e-mail Message-ID: <8CEE12B60147DDD-13BC-3A0F@webmail-d157.sysops.aol.com> From sllandsc <@t> gmail.com Thu Apr 5 08:20:20 2012 From: sllandsc <@t> gmail.com (Sara Landschoot) Date: Thu Apr 5 08:20:28 2012 Subject: [Histonet] Re: Massons Trichrome on decalcified bone Message-ID: Hi Gayle, Thanks so much for your response on Histonet. I have been getting several different answers that I have been trying to test. I can give you more details on how we decal our bone here. We deal with entire sheep or primate spines which are grossed while frozen into slabs. The slabs are about 5-6mm thick. We take an initial xray to get a starting point then we decal the slabs in EDTA (after formalin fixation). Xrays are taken throughout the decal process to check for the endpoint. As for post fixing in Bouins, I was using the water bath method (60 degrees) for about an hour. If you don't mind sending me the AFIP methods so I can try then out on my sections I would greatly appreciate it. My email is sllandsc@gmail.com. Again thank you for your help and I look forward to hearing from you. Sara Landschoot From SHUNTER <@t> beaumont.edu Thu Apr 5 08:23:57 2012 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Thu Apr 5 08:24:02 2012 Subject: [Histonet] RE: Leica Bond IHC Platform In-Reply-To: <5F46C87AD649864F9FA8273E8E31507A1607E0@SWM2006.LHSNT.LEGACYHS> References: <5F46C87AD649864F9FA8273E8E31507A1607E0@SWM2006.LHSNT.LEGACYHS> Message-ID: We have a Bond Max in my lab and love it! It has been very dependable and easy to use . We also have three Ventana Ultras and two Lab Visions. We originally got the Bond for EBV and Kappa/Lambda in-situ but also do our hormone receptors and a few other immunos on it. Our pathologists felt the signal obtained with the DAB on the in-situ slides was superior to the NBT of the Ventana slides. The one draw back that Ventana will talk about is that you have three racks of ten slides - each rack is independent of the others, but not the continual load of the Ultras. You also cannot mix pretreatments on each rack because of timing/heating issues. But we have not found either of these two issues to be a problem for us. The Bond III is supposed to be even faster than the Bond Max but haven't looked at that. Sue Sue Hunter Supervisor, Advanced Diagnostics Beaumont Health Systems Royal Oak MI 48073 248-898-5146 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wellen, Terrence D. :LPH Lab Sent: Wednesday, April 04, 2012 8:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond IHC Platform Does anyone have any experience with this product? Terrence Wellen HT(ASCP) Legacy Good Samaritan Hospital Portland, OR _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message has been scanned and no issues discovered. To report this email as SPAM, please forward it to spam@websense.com. From ratliffjack <@t> hotmail.com Thu Apr 5 09:06:32 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Apr 5 09:06:45 2012 Subject: [Histonet] Re: Massons Trichrome on decalcified bone In-Reply-To: References: Message-ID: Hello Sara! Might I quickly ask why you decalcify instead of processing and embedding undecalcified using methyl methacrylate resin/plastic? I routinely process these type of specimens into MMA and cut either 5 micron sections without metal present or thick/ground sections polished to 30-40 microns if a metallic device is present. These same specimens that have been processed and embedded into plastic and cut at 5 microns can be deplasticized prior to staining with H&E, Goldner's trichrome, VonKossa-MacNeal's tetrachrome, Safranin O-Fast Green, Sirius Red-Fast Green, etc. If these specimens contain a large metal implant, the sections are stained undeplasticized with Sanderson's Rapid Bone Stain & Van Gieson picrofuchsin. Of course of you don't have these capabilities or equipment to process these specimens into resin, decalcification is your only option, but I would personally use 5% or 10% Formic acid instead of the lengthy EDTA process. I would also use methyl salicylate to replace the xylenes steps so that you can avoid making the bone too brittle and difficult to cut. Feel free to message me back if you need further explanation or if you would even like to discuss privately by phone. I would also be happy to forward you stained images of these stains, previously listed and from this same specimen type you are working with, that has been resin embedded. Also, I have trained people all over the world to process undecalcified bone (any size) into resin/plastic and cut at 5 microns if this is an option for you! Best Regards, Jack Jack Ratliff Hard Tissue Histologist Chairman, Hard Tissue Committee - National Society for Histotechnology On Apr 5, 2012, at 9:20 AM, Sara Landschoot wrote: > Hi Gayle, > > Thanks so much for your response on Histonet. I have been getting several > different answers that I have been trying to test. > > I can give you more details on how we decal our bone here. We deal with > entire sheep or primate spines which are grossed while frozen into slabs. > The slabs are about 5-6mm thick. We take an initial xray to get a starting > point then we decal the slabs in EDTA (after formalin fixation). Xrays are > taken throughout the decal process to check for the endpoint. > > As for post fixing in Bouins, I was using the water bath method (60 > degrees) for about an hour. > > If you don't mind sending me the AFIP methods so I can try then out on my > sections I would greatly appreciate it. My email is sllandsc@gmail.com. > > Again thank you for your help and I look forward to hearing from you. > > Sara Landschoot > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From trathborne <@t> somerset-healthcare.com Thu Apr 5 09:50:52 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Apr 5 09:50:26 2012 Subject: [Histonet] RE: Leica Bond IHC Platform In-Reply-To: References: <5F46C87AD649864F9FA8273E8E31507A1607E0@SWM2006.LHSNT.LEGACYHS> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711FBB66D@SMCMAIL01.somerset-healthcare.com> We have the Bond III. No complaints. I'm not sure what to compare to regarding speed, but we can do 4 runs a day if necessary. This does not include an overnight run, which we do as needed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Thursday, April 05, 2012 9:24 AM To: Wellen, Terrence D. :LPH Lab; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Leica Bond IHC Platform We have a Bond Max in my lab and love it! It has been very dependable and easy to use . We also have three Ventana Ultras and two Lab Visions. We originally got the Bond for EBV and Kappa/Lambda in-situ but also do our hormone receptors and a few other immunos on it. Our pathologists felt the signal obtained with the DAB on the in-situ slides was superior to the NBT of the Ventana slides. The one draw back that Ventana will talk about is that you have three racks of ten slides - each rack is independent of the others, but not the continual load of the Ultras. You also cannot mix pretreatments on each rack because of timing/heating issues. But we have not found either of these two issues to be a problem for us. The Bond III is supposed to be even faster than the Bond Max but haven't looked at that. Sue Sue Hunter Supervisor, Advanced Diagnostics Beaumont Health Systems Royal Oak MI 48073 248-898-5146 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wellen, Terrence D. :LPH Lab Sent: Wednesday, April 04, 2012 8:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond IHC Platform Does anyone have any experience with this product? Terrence Wellen HT(ASCP) Legacy Good Samaritan Hospital Portland, OR _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message has been scanned and no issues discovered. To report this email as SPAM, please forward it to spam@websense.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From SHUNTER <@t> beaumont.edu Thu Apr 5 10:12:20 2012 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Thu Apr 5 10:12:25 2012 Subject: [Histonet] RE: Leica Bond IHC Platform In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711FBB66D@SMCMAIL01.somerset-healthcare.com> References: <5F46C87AD649864F9FA8273E8E31507A1607E0@SWM2006.LHSNT.LEGACYHS> <3AD061FE740D464FAC7BF6B5CFB7570711FBB66D@SMCMAIL01.somerset-healthcare.com> Message-ID: I've only been told that the Bond III could do a run in, say 3 hours, where the Bond Max might be done in 4.(don't take these times as true - just as an example). We love the ability to do a delayed start so the slides are done when we come in the next morning - especially the in-situ slides that take longer than the immunos. sue -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Thursday, April 05, 2012 10:51 AM To: Sue Hunter; Wellen, Terrence D. :LPH Lab; histonet@lists.utsouthwestern.edu Subject: RE: Leica Bond IHC Platform We have the Bond III. No complaints. I'm not sure what to compare to regarding speed, but we can do 4 runs a day if necessary. This does not include an overnight run, which we do as needed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Thursday, April 05, 2012 9:24 AM To: Wellen, Terrence D. :LPH Lab; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Leica Bond IHC Platform We have a Bond Max in my lab and love it! It has been very dependable and easy to use . We also have three Ventana Ultras and two Lab Visions. We originally got the Bond for EBV and Kappa/Lambda in-situ but also do our hormone receptors and a few other immunos on it. Our pathologists felt the signal obtained with the DAB on the in-situ slides was superior to the NBT of the Ventana slides. The one draw back that Ventana will talk about is that you have three racks of ten slides - each rack is independent of the others, but not the continual load of the Ultras. You also cannot mix pretreatments on each rack because of timing/heating issues. But we have not found either of these two issues to be a problem for us. The Bond III is supposed to be even faster than the Bond Max but haven't looked at that. Sue Sue Hunter Supervisor, Advanced Diagnostics Beaumont Health Systems Royal Oak MI 48073 248-898-5146 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wellen, Terrence D. :LPH Lab Sent: Wednesday, April 04, 2012 8:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond IHC Platform Does anyone have any experience with this product? Terrence Wellen HT(ASCP) Legacy Good Samaritan Hospital Portland, OR _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message has been scanned and no issues discovered. To report this email as SPAM, please forward it to spam@websense.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. This message has been scanned and no issues discovered. To report this email as SPAM, please forward it to spam@websense.com. From b427297 <@t> aol.com Thu Apr 5 10:18:53 2012 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Thu Apr 5 10:19:03 2012 Subject: [Histonet] Commercial clearing and bluing solutions in Europe In-Reply-To: References: <5F46C87AD649864F9FA8273E8E31507A1607E0@SWM2006.LHSNT.LEGACYHS><3AD061FE740D464FAC7BF6B5CFB7570711FBB66D@SMCMAIL01.somerset-healthcare.com> Message-ID: <8CEE1568C202EBD-1A70-18B07@webmail-m011.sysops.aol.com> I am looking for vendors in Germany who can provide commercially prepared, RTU H+E clarifier and bluing reagents. Any advice? Leica does not distribute these products in Germany, I've been told. Thanks, Jackie O' From Jeffrey.Howery <@t> jcl.com Thu Apr 5 10:36:46 2012 From: Jeffrey.Howery <@t> jcl.com (Jeffrey Howery) Date: Thu Apr 5 10:36:53 2012 Subject: [Histonet] JOB OPENING Message-ID: <40985CBB26DB2F4D8FBC273E835992E79625@JCLNSEXMAIL02.jclnt.jcl.com> I am just putting this out there but we have an opening here at John C Lincoln Deer Valley Phoenix, Arizona. If anyone might be interested please contact me. I can help fill you in on what we are looking for. Thanks Jeff From kalschev <@t> svm.vetmed.wisc.edu Thu Apr 5 12:15:51 2012 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Thu Apr 5 12:16:01 2012 Subject: [Histonet] Re:SAVE MAsson's in BONE info References: Message-ID: ----- Original Message ----- From: To: Sent: Thursday, April 05, 2012 12:05 PM Subject: Histonet Digest, Vol 101, Issue 4 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Slippery Floor due to paraffin (Sebree Linda A) > 2. New Position Alert - IHC Field Tech Atlanta (Matt Ward) > 3. Glassware washing (Oneil, Beth Ann) > 4. Re: Massons Trichrome on decalcified bone (gayle callis) > 5. Automatic knife sharpener (Maxim Peshkov) > 6. Grossing rules (Amber McKenzie) > 7. EM tissue processors Q's (Morken, Timothy) > 8. Specimen Identity Procedure (Scott, Allison D) > 9. Re: EM tissue processors Q's (Jan Shivers) > 10. Bone (Trichrome) (Lawrence Allen) > 11. Region III final reminder (Shirley A. Powell) > 12. Leica Bond IHC Platform (Wellen, Terrence D. :LPH Lab) > 13. (no subject) (Khaire Dai) > 14. please don't sent e-mail (mervatawad@aol.com) > 15. Re: Massons Trichrome on decalcified bone (Sara Landschoot) > 16. RE: Leica Bond IHC Platform (Sue Hunter) > 17. Re: Re: Massons Trichrome on decalcified bone (Jack Ratliff) > 18. RE: Leica Bond IHC Platform (Rathborne, Toni) > 19. RE: Leica Bond IHC Platform (Sue Hunter) > 20. Commercial clearing and bluing solutions in Europe > (Jackie O'Connor) > 21. JOB OPENING (Jeffrey Howery) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 4 Apr 2012 12:21:24 -0500 > From: "Sebree Linda A" > Subject: RE: [Histonet] Slippery Floor due to paraffin > To: "Scott, Allison D" , > > Message-ID: > > > Content-Type: text/plain; charset="US-ASCII" > > Peel off film here...works well. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, > Allison D > Sent: Wednesday, April 04, 2012 11:50 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slippery Floor due to paraffin > > Hello to all in histoland. What are histology labs doing to combat the > slipperiness of the floor due to paraffin. Are you using rugs, peel > away films ? Any help would be greatly appreciated. > > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas 77026 > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance > Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR > Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is > confidential and/or privileged. This e-mail may also be confidential > and/or privileged under Texas law. The e-mail is for the use of only > the individual or entity named above. If you are not the intended > recipient, or any authorized representative of the intended recipient, > you are hereby notified that any review, dissemination or copying of > this e-mail and its attachments is strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Wed, 4 Apr 2012 13:31:10 -0400 > From: Matt Ward > Subject: [Histonet] New Position Alert - IHC Field Tech Atlanta > To: histonet@lists.utsouthwestern.edu > Message-ID: <33b696097ef326f96a51a0f52df63358@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Good Afternoon Histonet, > > > > We have had a global leader in IHC and Histology open a field based IHC > support opportunity covering the Southeast Region. The ideal location > would > be to be based in Atlanta and the position is open due to promotion. > > > > If you or anyone you may know has a strong background in IHC and is > looking > to break out of the lab into a field role please contact me directly to > learn more. > > > > The position offers a Base Salary + Bonus + Full Benefits (Car Allowance, > Corporate Credit Card, Cell Phone, Laptop, Home Office, Full Health, > 401k). > > > > Regards, > > > > > > Matt Ward > > *Account Executive* > > *Personify* > > 5020 Weston Parkway Suite 315 > > Cary NC 27513 > > (Tel) 800.875.6188 direct ext 103 > > (Fax) 919.460.0642 > > www.personifysearch.com > > > ------------------------------ > > Message: 3 > Date: Wed, 4 Apr 2012 18:08:14 +0000 > From: "Oneil, Beth Ann" > Subject: [Histonet] Glassware washing > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <3CEB8EBCF9C7A648B9694B5696462A71EFE0@NT-EXMB2.wvuh.wvuhs.com> > Content-Type: text/plain; charset="us-ascii" > > Our laboratory does about half of it's daily special stains manually which > means we go through a lot of Copeland jars. We currently wash everything > by hand using liquinox with a splash of bleach in the water. We rinse > with tap water. My question is how do other labs address the CAP issue of > testing their glassware for detergent removal. When I worked in the > chemistry lab, we didn't go through a lot of glassware so we were able to > take a monthly bin of dirty glassware to sterile processing for cleaning, > and then just check one representative piece of glassware from the batch > for detergent. I can't figure out how my lab can check our glassware when > there is so much, we wash it all throughout the day. Thank you for any > help. > > Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC > Histology Supervisor/Technical Specialist > West Virginia University Hospitals > 304-293-7629 (office) > 304-293-6014 (lab) > > > > > > ------------------------------ > > Message: 4 > Date: Wed, 4 Apr 2012 12:13:26 -0600 > From: "gayle callis" > Subject: [Histonet] Re: Massons Trichrome on decalcified bone > To: "'Histonet'" > Message-ID: <000a01cd128e$a30c7110$e9255330$@bresnan.net> > Content-Type: text/plain; charset="US-ASCII" > > Dear Sara, > > > > You wrote: > > I work at an Orthopaedic research lab and I have been having some trouble > getting our trichromes to work on our bone. I have tried a masson's, > gomori, > and goldner's and in all cases the bone stained red. > > > > Our bone is arrive to our lab frozen, we fix them in formalin, embed in > paraffin, and decal with EDTA. I also post fix with Bouins before > staining. > > > > Techs that worked in this lab before me were also getting the same red > staining of bone. > > > > Is there something during processing that could cause this reaction? We > fix > in formalin, dehydrate through graded alcohols, and clear in xylene. > > > > **************************************************************************** > **************************** > > I doubt your processing has any effect on Mass Tri staining. However, > incomplete decalcification can but I suspect it may be the staining > protocol > itself. Using a simple weight loss/weight gain decalcification end point > test with EDTA is a good idea, and can be used for acid decalcification > endpoint testing IF you are not presently using an endpoint test. > > > > Years ago, I visited the AFIP bone lab, and acquired a Massons Trichrome > protocol which worked much better than the standard Massons Trichrome > found > in most textbooks. It never failed to work well with our decalcified bone > work although we did use acid decalcification. This is NOT a kit > method. > All reagents are made up in house, and post mordant heating Bouins is NOT > done in a microwave. We preferred to let the sections sit in Boiuns > overnight at RT, or heat in a water bath. We never used a dry heat, > incubator type oven where one gets uneven heating in the chamber. > > > > There is more to doing Massons Trichrome on decalcified bone other than > just > following the recipe from a textbook. One of the best discussions on > understanding the chemistry/theory of trichrome staining is found in > Sheehan > and Hrapchak, Theory and Practice of Histotechnology. > > > > The AFIP method has some different staining times, plus how to remove the > biebrich scarlet/acid fuchsin from collagen BEFORE going into aniline > blue, > a much more controlled staining method. It also has a modified Weigerts > Iron hematoxylin that is superior, a bit more concentrated since Iron > hematoxylin tends to be removed by the acidic staining solutions. I will > be happy to send these methods to you privately. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > ------------------------------ > > Message: 5 > Date: Wed, 4 Apr 2012 23:20:15 +0300 > From: Maxim Peshkov > Subject: [Histonet] Automatic knife sharpener > To: histonet@lists.utsouthwestern.edu > Message-ID: <1182039392.20120404232015@mail.ru> > Content-Type: text/plain; charset=windows-1251 > > Dear Histonetters! > Can anybody to advise, what type automatic knife sharpener > we can buy in Europe (Germany) instead Leica SP9000, > which now is unavailable for sale? What vendors? > We do not like a Shandon "Autosharp 5" type sharpener. > Maxim Peshkov > Russia, > Taganrog. > mailto:Maxim_71@mail.ru > > > > > ------------------------------ > > Message: 6 > Date: Wed, 4 Apr 2012 19:26:06 +0000 > From: Amber McKenzie > Subject: [Histonet] Grossing rules > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: <5A33C952BB67F4468AF1F36D739212BC11631D48@JERRY.Gia.com> > Content-Type: text/plain; charset="us-ascii" > > > Are there any guidelines on how to do GI grossing? At what size to you > bisect? Do you call 5 or more pieces multiple or do you count all the > pieces? How many categories are there: 1, 2, 3, several, multiple, > etc...At what size is it considered a fragment and what size is a polyp? > Thanks! > > > > ------------------------------ > > Message: 7 > Date: Wed, 4 Apr 2012 12:26:19 -0700 > From: "Morken, Timothy" > Subject: [Histonet] EM tissue processors Q's > To: Histonet > Message-ID: > <8D7C2D242DBD45498006B21122072BF8A4DDF412@MCINFRWEM003.ucsfmedicalcenter.org> > > Content-Type: text/plain; charset=us-ascii > > Hi, we're looking around at different automated TEM tissue processors. We > have an RMC 5160 but it has had a lot of problems so we are considering > something else. I've looked online at Leica TP and Lynx II for routine > processing. We may get to MW processing later, but the price is higher > than authorized right now. > > Any suggestions or comments? > > Thanks for any info! > > > Tim Morken > Supervisor, Electron Microscopy > Department of Pathology > UC San Francisco Medical Center > 505 Parnassus Ave, Box 1656 > Room S570 > San Francisco, CA 94115 > > (415) 353-2673 (ph) > (415) 514-3403 (fax) > tim.morken@ucsfmedctr.org > > > > > ------------------------------ > > Message: 8 > Date: Wed, 4 Apr 2012 19:57:46 +0000 > From: "Scott, Allison D" > Subject: [Histonet] Specimen Identity Procedure > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hello again to all in histoland. Does anyone have a procedure for > describing the system for maintaining the identity of every specimen > through processing and block and slide preparation. This is on the CAP > checklist (ANP.21050). Any help will be greatly appreciated. > > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance > Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential > and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity > named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > > > ------------------------------ > > Message: 9 > Date: Wed, 4 Apr 2012 15:05:50 -0500 > From: Jan Shivers > Subject: Re: [Histonet] EM tissue processors Q's > To: "Morken, Timothy" > Cc: Histonet > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > We use the Leica TP for our samples and endorse it. > > Jan Shivers > IHC/Histo/EM Section Head > Veterinary Diagnostic Lab > UMN College of Veterinary Medicine > St. Paul, MN > > On Wed, Apr 4, 2012 at 2:26 PM, Morken, Timothy < > Timothy.Morken@ucsfmedctr.org> wrote: > >> Hi, we're looking around at different automated TEM tissue processors. We >> have an RMC 5160 but it has had a lot of problems so we are considering >> something else. I've looked online at Leica TP and Lynx II for routine >> processing. We may get to MW processing later, but the price is higher >> than >> authorized right now. >> >> Any suggestions or comments? >> >> Thanks for any info! >> >> >> Tim Morken >> Supervisor, Electron Microscopy >> Department of Pathology >> UC San Francisco Medical Center >> 505 Parnassus Ave, Box 1656 >> Room S570 >> San Francisco, CA 94115 >> >> (415) 353-2673 (ph) >> (415) 514-3403 (fax) >> tim.morken@ucsfmedctr.org >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > ------------------------------ > > Message: 10 > Date: Wed, 4 Apr 2012 17:03:44 -0400 > From: Lawrence Allen > Subject: [Histonet] Bone (Trichrome) > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset=us-ascii > > > Why not sent in 10 % formalin. Freezing it may change the morphology of > the bone to fixation after thawing. I would give that a try. After all, > you are running 3 different stains at one time in order. > > Lawrence S Allen > Lead Histotechnologist > Dorm VA Medical Center > Columbia, SC > > > > > ------------------------------ > > Message: 11 > Date: Wed, 4 Apr 2012 17:54:42 -0400 > From: "Shirley A. Powell" > Subject: [Histonet] Region III final reminder > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <9BF995BC0E47744E9673A41486E24EE24B330F1C3D@MERCERMAIL.MercerU.local> > Content-Type: text/plain; charset="us-ascii" > > Hi Histotechs, > > This email is to remind you of the Georgia Society for Histotechnology > hosting Region III meeting at Callaway Gardens, Pine Mountain GA April > 13-15th,. This is next week friends. We will NOT be in the conference > center as last year, and that is a GOOD thing. We will be in the ballroom > area where we will have more space. We have far exceeded our original > block of rooms, extended the deadline and now if the Mountain Creek Inn > has any available, they will allow the GSH room rate. Please use the GSH > Group # # 78K711 to get the discounted room rate of $109. Make > reservations now at 1-800-225-5292. > > If you have not registered for the meeting please do so. PLEASE > REGISTER NOW AS "AWAITING FUNDS" if you are waiting approval for the > meeting go ahead and register for your workshops, luncheon, dinner and > note on the form that you are awaiting funds below the total line. When > approved, then send the funds, or if time does not allow, pay on site. > But please register as soon as possible, don't wait, workshops are filling > up fast. > > We also will have Histotalk's Dave Kemler at the meeting to interview > some of our attendees as well. > > Also make plans to attend the Carriage and Horses Dinner on Saturday > night for great food, great friends and good entertainment. The deadline > for reservations for the dinner is April 9th, that is next Monday. Please > make sure you fill out your registration form completely and email the > form to Anne Taylor, GSH Treasurer to confirm your workshops as awaiting > funds and then pay at the registration desk on site since time is short. > > If you have questions or concerns please contact Mike Ayers at > lmayers@charter.net, Wanda Simons at > Wandrous@att.net or myself at > powell_sa@mercer.edu. > > We are excited to have 29 vendors exhibiting at our meeting and more > signing up as we approach the meeting. > > BioCare ~Sponsor of new IHC Award > > BioGenex > > B/R Instruments > > Cancer Diagnostics > > Cell Marque > > Choice Medical > > Clarient~ sponsoring dinner at Dagher's > > Dako > > EMS > > Epitomics > > General Data > > IMEB > > Lab Storage > > Leica Microsystems ~ sponsoring dinner at Dagher's > > Leica Biosystems > > Mopec > > PolyScientific R&D - sponsor of new award "TBA" > > Sakura - Sponsor of GSH Histotechnologist of the Year Award > > Stat Lab > > Southeast Pathology > > Thermo Fisher - > > TBS > > ScyTek > > StatLab > > Ventana > > > > Those Not attending but supporting GSH are: > > Anatech - Unmanned table > > CL Sturkey - Door prizes > > LABSCO - Sponsoring a break > > Newcomer - Sponsoring a Break and provided totes > > Come Experience ____________ > Histotechnology - Southern Style > > > > > > ------------------------------ > > Message: 12 > Date: Wed, 4 Apr 2012 17:06:04 -0700 > From: "Wellen, Terrence D. :LPH Lab" > Subject: [Histonet] Leica Bond IHC Platform > To: > Message-ID: > <5F46C87AD649864F9FA8273E8E31507A1607E0@SWM2006.LHSNT.LEGACYHS> > Content-Type: text/plain; charset="iso-8859-1" > > > Does anyone have any experience with this product? > > > Terrence Wellen HT(ASCP) > Legacy Good Samaritan Hospital > Portland, OR > > > > ------------------------------ > > Message: 13 > Date: Wed, 4 Apr 2012 20:46:34 -0700 (PDT) > From: Khaire Dai > Subject: [Histonet] (no subject) > To: histonet@lists.utsouthwestern.edu, kgrobert@rci.rutgers.edu > Message-ID: > <1333597594.50037.YahooMailMobile@web113010.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > href="http://gearsnow.com/wp-content/plugins/extended-comment-options/fjgvkd.html"> > http://gearsnow.com/wp-content/plugins/extended-comment-options/fjgvkd.html > > ------------------------------ > > Message: 14 > Date: Thu, 5 Apr 2012 06:09:51 -0400 (EDT) > From: mervatawad@aol.com > Subject: [Histonet] please don't sent e-mail > To: Histonet@lists.utsouthwestern.edu > Message-ID: <8CEE12B60147DDD-13BC-3A0F@webmail-d157.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > > > ------------------------------ > > Message: 15 > Date: Thu, 5 Apr 2012 08:20:20 -0500 > From: Sara Landschoot > Subject: [Histonet] Re: Massons Trichrome on decalcified bone > To: Histonet > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Gayle, > > Thanks so much for your response on Histonet. I have been getting several > different answers that I have been trying to test. > > I can give you more details on how we decal our bone here. We deal with > entire sheep or primate spines which are grossed while frozen into slabs. > The slabs are about 5-6mm thick. We take an initial xray to get a starting > point then we decal the slabs in EDTA (after formalin fixation). Xrays are > taken throughout the decal process to check for the endpoint. > > As for post fixing in Bouins, I was using the water bath method (60 > degrees) for about an hour. > > If you don't mind sending me the AFIP methods so I can try then out on my > sections I would greatly appreciate it. My email is sllandsc@gmail.com. > > Again thank you for your help and I look forward to hearing from you. > > Sara Landschoot > > > ------------------------------ > > Message: 16 > Date: Thu, 5 Apr 2012 13:23:57 +0000 > From: Sue Hunter > Subject: [Histonet] RE: Leica Bond IHC Platform > To: "Wellen, Terrence D. :LPH Lab" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > We have a Bond Max in my lab and love it! It has been very dependable and > easy to use . We also have three Ventana Ultras and two Lab Visions. We > originally got the Bond for EBV and Kappa/Lambda in-situ but also do our > hormone receptors and a few other immunos on it. Our pathologists felt > the signal obtained with the DAB on the in-situ slides was superior to the > NBT of the Ventana slides. The one draw back that Ventana will talk about > is that you have three racks of ten slides - each rack is independent of > the others, but not the continual load of the Ultras. You also cannot mix > pretreatments on each rack because of timing/heating issues. But we have > not found either of these two issues to be a problem for us. The Bond III > is supposed to be even faster than the Bond Max but haven't looked at > that. > Sue > > Sue Hunter > Supervisor, Advanced Diagnostics > Beaumont Health Systems > Royal Oak MI 48073 > 248-898-5146 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wellen, > Terrence D. :LPH Lab > Sent: Wednesday, April 04, 2012 8:06 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica Bond IHC Platform > > > Does anyone have any experience with this product? > > > Terrence Wellen HT(ASCP) > Legacy Good Samaritan Hospital > Portland, OR > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This message has been scanned and no issues discovered. > To report this email as SPAM, please forward it to spam@websense.com. > > > > ------------------------------ > > Message: 17 > Date: Thu, 5 Apr 2012 10:06:32 -0400 > From: Jack Ratliff > Subject: Re: [Histonet] Re: Massons Trichrome on decalcified bone > To: Sara Landschoot > Cc: Histonet > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Hello Sara! > > Might I quickly ask why you decalcify instead of processing and embedding > undecalcified using methyl methacrylate resin/plastic? I routinely process > these type of specimens into MMA and cut either 5 micron sections without > metal present or thick/ground sections polished to 30-40 microns if a > metallic device is present. > > These same specimens that have been processed and embedded into plastic > and cut at 5 microns can be deplasticized prior to staining with H&E, > Goldner's trichrome, VonKossa-MacNeal's tetrachrome, Safranin O-Fast > Green, Sirius Red-Fast Green, etc. If these specimens contain a large > metal implant, the sections are stained undeplasticized with Sanderson's > Rapid Bone Stain & Van Gieson picrofuchsin. > > Of course of you don't have these capabilities or equipment to process > these specimens into resin, decalcification is your only option, but I > would personally use 5% or 10% Formic acid instead of the lengthy EDTA > process. I would also use methyl salicylate to replace the xylenes steps > so that you can avoid making the bone too brittle and difficult to cut. > > Feel free to message me back if you need further explanation or if you > would even like to discuss privately by phone. I would also be happy to > forward you stained images of these stains, previously listed and from > this same specimen type you are working with, that has been resin > embedded. Also, I have trained people all over the world to process > undecalcified bone (any size) into resin/plastic and cut at 5 microns if > this is an option for you! > > Best Regards, > > Jack > > Jack Ratliff > Hard Tissue Histologist > Chairman, Hard Tissue Committee - National Society for Histotechnology > > On Apr 5, 2012, at 9:20 AM, Sara Landschoot wrote: > >> Hi Gayle, >> >> Thanks so much for your response on Histonet. I have been getting several >> different answers that I have been trying to test. >> >> I can give you more details on how we decal our bone here. We deal with >> entire sheep or primate spines which are grossed while frozen into slabs. >> The slabs are about 5-6mm thick. We take an initial xray to get a >> starting >> point then we decal the slabs in EDTA (after formalin fixation). Xrays >> are >> taken throughout the decal process to check for the endpoint. >> >> As for post fixing in Bouins, I was using the water bath method (60 >> degrees) for about an hour. >> >> If you don't mind sending me the AFIP methods so I can try then out on my >> sections I would greatly appreciate it. My email is sllandsc@gmail.com. >> >> Again thank you for your help and I look forward to hearing from you. >> >> Sara Landschoot >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > ------------------------------ > > Message: 18 > Date: Thu, 5 Apr 2012 14:50:52 +0000 > From: "Rathborne, Toni" > Subject: [Histonet] RE: Leica Bond IHC Platform > To: "'Sue Hunter'" , "Wellen, Terrence D. :LPH > Lab" , "histonet@lists.utsouthwestern.edu" > > Message-ID: > <3AD061FE740D464FAC7BF6B5CFB7570711FBB66D@SMCMAIL01.somerset-healthcare.com> > > Content-Type: text/plain; charset="us-ascii" > > We have the Bond III. No complaints. I'm not sure what to compare to > regarding speed, but we can do 4 runs a day if necessary. This does not > include an overnight run, which we do as needed. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter > Sent: Thursday, April 05, 2012 9:24 AM > To: Wellen, Terrence D. :LPH Lab; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Leica Bond IHC Platform > > We have a Bond Max in my lab and love it! It has been very dependable and > easy to use . We also have three Ventana Ultras and two Lab Visions. We > originally got the Bond for EBV and Kappa/Lambda in-situ but also do our > hormone receptors and a few other immunos on it. Our pathologists felt > the signal obtained with the DAB on the in-situ slides was superior to the > NBT of the Ventana slides. The one draw back that Ventana will talk about > is that you have three racks of ten slides - each rack is independent of > the others, but not the continual load of the Ultras. You also cannot mix > pretreatments on each rack because of timing/heating issues. But we have > not found either of these two issues to be a problem for us. The Bond III > is supposed to be even faster than the Bond Max but haven't looked at > that. > Sue > > Sue Hunter > Supervisor, Advanced Diagnostics > Beaumont Health Systems > Royal Oak MI 48073 > 248-898-5146 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wellen, > Terrence D. :LPH Lab > Sent: Wednesday, April 04, 2012 8:06 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica Bond IHC Platform > > > Does anyone have any experience with this product? > > > Terrence Wellen HT(ASCP) > Legacy Good Samaritan Hospital > Portland, OR > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This message has been scanned and no issues discovered. > To report this email as SPAM, please forward it to spam@websense.com. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in > this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > > > ------------------------------ > > Message: 19 > Date: Thu, 5 Apr 2012 15:12:20 +0000 > From: Sue Hunter > Subject: [Histonet] RE: Leica Bond IHC Platform > To: "Rathborne, Toni" , "Wellen, > Terrence D. :LPH Lab" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > I've only been told that the Bond III could do a run in, say 3 hours, > where the Bond Max might be done in 4.(don't take these times as true - > just as an example). We love the ability to do a delayed start so the > slides are done when we come in the next morning - especially the in-situ > slides that take longer than the immunos. > sue > > -----Original Message----- > From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] > Sent: Thursday, April 05, 2012 10:51 AM > To: Sue Hunter; Wellen, Terrence D. :LPH Lab; > histonet@lists.utsouthwestern.edu > Subject: RE: Leica Bond IHC Platform > > We have the Bond III. No complaints. I'm not sure what to compare to > regarding speed, but we can do 4 runs a day if necessary. This does not > include an overnight run, which we do as needed. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter > Sent: Thursday, April 05, 2012 9:24 AM > To: Wellen, Terrence D. :LPH Lab; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Leica Bond IHC Platform > > We have a Bond Max in my lab and love it! It has been very dependable and > easy to use . We also have three Ventana Ultras and two Lab Visions. We > originally got the Bond for EBV and Kappa/Lambda in-situ but also do our > hormone receptors and a few other immunos on it. Our pathologists felt > the signal obtained with the DAB on the in-situ slides was superior to the > NBT of the Ventana slides. The one draw back that Ventana will talk about > is that you have three racks of ten slides - each rack is independent of > the others, but not the continual load of the Ultras. You also cannot mix > pretreatments on each rack because of timing/heating issues. But we have > not found either of these two issues to be a problem for us. The Bond III > is supposed to be even faster than the Bond Max but haven't looked at > that. > Sue > > Sue Hunter > Supervisor, Advanced Diagnostics > Beaumont Health Systems > Royal Oak MI 48073 > 248-898-5146 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wellen, > Terrence D. :LPH Lab > Sent: Wednesday, April 04, 2012 8:06 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica Bond IHC Platform > > > Does anyone have any experience with this product? > > > Terrence Wellen HT(ASCP) > Legacy Good Samaritan Hospital > Portland, OR > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This message has been scanned and no issues discovered. > To report this email as SPAM, please forward it to spam@websense.com. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in > this message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk at > 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, event > listings, health information and more. > This message has been scanned and no issues discovered. > To report this email as SPAM, please forward it to spam@websense.com. > > > > ------------------------------ > > Message: 20 > Date: Thu, 5 Apr 2012 11:18:53 -0400 (EDT) > From: "Jackie O'Connor" > Subject: [Histonet] Commercial clearing and bluing solutions in Europe > To: histonet@lists.utsouthwestern.edu > Message-ID: <8CEE1568C202EBD-1A70-18B07@webmail-m011.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > > I am looking for vendors in Germany who can provide commercially prepared, > RTU H+E clarifier and bluing reagents. Any advice? Leica does not > distribute these products in Germany, I've been told. > Thanks, > Jackie O' > > > ------------------------------ > > Message: 21 > Date: Thu, 5 Apr 2012 15:36:46 +0000 > From: Jeffrey Howery > Subject: [Histonet] JOB OPENING > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <40985CBB26DB2F4D8FBC273E835992E79625@JCLNSEXMAIL02.jclnt.jcl.com> > Content-Type: text/plain; charset="us-ascii" > > I am just putting this out there but we have an opening here at John C > Lincoln Deer Valley Phoenix, Arizona. > If anyone might be interested please contact me. I can help fill you in on > what we are looking for. > Thanks Jeff > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 101, Issue 4 > **************************************** > From rsrichmond <@t> gmail.com Thu Apr 5 12:30:42 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Apr 5 12:30:45 2012 Subject: [Histonet] Re: Grossing rules Message-ID: Amber McKenzie asks: >>Are there any guidelines on how to do GI grossing? At what size to you bisect? Do you call 5 or more pieces multiple or do you count all the pieces? How many categories are there: 1, 2, 3, several, multiple, etc.? At what size is it considered a fragment and what size is a polyp?<< This is very poorly standardized, and basically I can tell you my own practice. I bisect polyps 4 mm in size or greater - that's about as steady as my hands are. I count all the pieces up to about 10. I don't understand the question about categories. It's a polyp if it looks like a polyp. I don't measure specimens of ordinary size, since I cannot do it accurately. I'll sometimes refer to "a single quite small specimen." Examples of dictation: 1234. Received in fixative labeled "John Doe" and "duodenum" is a single biopsy specimen submitted in cassette A. 2345. Received in fixative labeled "Mary Doe" and "esophagus" are four biopsy specimens submitted together in cassette A. 3456. Received in fixative labeled "John Roe" and "transverse colon" is a single 5 mm dull red polyp with a very short stalk, divided longitudinally into two pieces, and entirely submitted in cassette A. As I said, that's just what I do when I gross for myself. Bob Richmond Samurai Pathologist Knoxville TN Received in fixative is a single biopsy specimen submitted in cassette A. From brannon <@t> alliedsearchpartners.com Thu Apr 5 14:05:14 2012 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Thu Apr 5 14:05:29 2012 Subject: [Histonet] Histology Openings for full and part time Message-ID: Allied Search Partners currently has openings for the following (full job descriptions available): Mohs Histotech- Denver, CO area- full time Histotechnician or Histotechnologist ? Portland, OR area- part time Interested candidates should submit their updated resumes to brannon@alliedsearchpartners.com. No more information will be given about location of the lab at this time. Thank you. -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From ctorrence <@t> kmcpa.com Thu Apr 5 14:16:56 2012 From: ctorrence <@t> kmcpa.com (Carol Torrence) Date: Thu Apr 5 14:17:19 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs Message-ID: <004601cd1360$ab3492f0$019db8d0$@com> We have received notification from AETNA that they now require non-hospital labs to be accredited by CLIA and CAP. The letter makes it obvious that by making such a request that they are not aware that CLIA assigned deemed status to CAP and CLIA is actually the gatekeeper. Secondly we are told to be registered by May 1st and accredited by August 1st (which CAP says is impossible) or we will have to send our lab to either Quest or Ameripath which includes Dermpath Diagnostics division. It fails to mention that there are other CAP accredited non hospital labs in our state. The Aetna contact number is either 'mailbox full" or even after leaving a message, no return call. Me thinks me smells a rat. If you are a non-hospital lab, have you heard of this? Does your dematopathologist or pathologist know this is coming? I am interested in your comments. Carol M. Torrence, HT(ASCP)CM From portera <@t> msu.edu Thu Apr 5 14:40:25 2012 From: portera <@t> msu.edu (Amy Porter) Date: Thu Apr 5 14:40:29 2012 Subject: [Histonet] Loss of Matrigel material during staining Message-ID: <000a01cd1363$f2df1a50$d89d4ef0$@edu> To all: I have a client that we are attempting to stain H & E / as well as immuno on FFPE mouse skin with matrigel plugs. These things section like a dream and then when we attempt to stain only the matrigel part of the sample is falling off. Does anyone have any tricks with this stuff they would be willing to share? We are using charged slides and cutting at 5-6 microns per the client. Thanks - Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory William S. Spielman, Ph.D. - Director Patricia K. Senagore, M.D. - Consulting Pathologist Department of Physiology / Human Pathology Biomedical Physical Sciences Building 567 Wilson Road - Room 2133 East Lansing, MI 48824-3320 Phone: 517-884-5026 Fax: 517-432-1368 portera@msu.edu www.humanpathology.msu.edu From TroyerDA <@t> EVMS.EDU Thu Apr 5 15:02:33 2012 From: TroyerDA <@t> EVMS.EDU (Troyer, Dean A.) Date: Thu Apr 5 15:03:48 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs References: <004601cd1360$ab3492f0$019db8d0$@com> Message-ID: <9A3AF60951E04547B598B76A5CCC5C1803F8A987@vulcan.evms.net> Was the notification in the form of a letter (snail mail) or another form such as email? Dean Troyer ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Carol Torrence Sent: Thu 4/5/2012 3:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs We have received notification from AETNA that they now require non-hospital labs to be accredited by CLIA and CAP. The letter makes it obvious that by making such a request that they are not aware that CLIA assigned deemed status to CAP and CLIA is actually the gatekeeper. Secondly we are told to be registered by May 1st and accredited by August 1st (which CAP says is impossible) or we will have to send our lab to either Quest or Ameripath which includes Dermpath Diagnostics division. It fails to mention that there are other CAP accredited non hospital labs in our state. The Aetna contact number is either 'mailbox full" or even after leaving a message, no return call. Me thinks me smells a rat. If you are a non-hospital lab, have you heard of this? Does your dematopathologist or pathologist know this is coming? I am interested in your comments. Carol M. Torrence, HT(ASCP)CM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt <@t> uphs.upenn.edu Thu Apr 5 15:04:57 2012 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Thu Apr 5 15:04:44 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <004601cd1360$ab3492f0$019db8d0$@com> References: <004601cd1360$ab3492f0$019db8d0$@com> Message-ID: Actually, I called the contact person on the letter and received a call back within 24 hours. I asked her if TJC and COLA would be acceptable in lieu of CAP and she is going to get an answer to me by next week. I am hoping the goal of this is simply to ensure and support high quality patient care, but I agree that the letter should not read as an advertisement for "preferred laboratories". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Torrence Sent: Thursday, April 05, 2012 3:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs We have received notification from AETNA that they now require non-hospital labs to be accredited by CLIA and CAP. The letter makes it obvious that by making such a request that they are not aware that CLIA assigned deemed status to CAP and CLIA is actually the gatekeeper. Secondly we are told to be registered by May 1st and accredited by August 1st (which CAP says is impossible) or we will have to send our lab to either Quest or Ameripath which includes Dermpath Diagnostics division. It fails to mention that there are other CAP accredited non hospital labs in our state. The Aetna contact number is either 'mailbox full" or even after leaving a message, no return call. Me thinks me smells a rat. If you are a non-hospital lab, have you heard of this? Does your dematopathologist or pathologist know this is coming? I am interested in your comments. Carol M. Torrence, HT(ASCP)CM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From mikoff <@t> pacbell.net Thu Apr 5 15:22:09 2012 From: mikoff <@t> pacbell.net (Keith) Date: Thu Apr 5 15:22:13 2012 Subject: [Histonet] 5. Automatic knife sharpener, Histonet Digest, Vol 101, Issue 4 In-Reply-To: <201204051705.q35H5psg013095@nlpi112.prodigy.net> References: <201204051705.q35H5psg013095@nlpi112.prodigy.net> Message-ID: <1333657329.8863.YahooMailRC@web184517.mail.ne1.yahoo.com> Maxim, I have used and would recommend the Hacker H/I-76 Microtome Knife Sharpener.? With this sharpener, honing and stropping a microtome knife?takes only a few?minutes,?and?results in?a better knife edge than the automatic glass plate honing/stropping method.? Also, as long as the knife edge has not been nicked or damaged, the microtome knife need only be stropped as needed.? ??I like you choice to stay away from the disposable knife blades.? I preferred using the solid knife to the disposable blades because of the strength and stability at the cutting edge (less of a chance for the chatter artifact).? The very sharp, slightly biconcave edge on the blade (see profile B on their website, below) allows 4 micron sections to be easily sectioned from the paraffin block. ?A new H/I-76?can be ordered directly from Hacker,? http://www.hackerinstruments.com/tissueprep.htm.?? For less of an investment... be wary,?there are a few?available on the secondary market. ? Keith Mikoff, HTL/HT(ASCP) Sacramento, California mikoff@gmail.com Message: 5 Date: Wed, 4 Apr 2012 23:20:15 +0300 From: Maxim Peshkov Subject: [Histonet] Automatic knife sharpener To: histonet@lists.utsouthwestern.edu Message-ID: <1182039392.20120404232015@mail.ru> Content-Type: text/plain; charset=windows-1251 Dear Histonetters! Can anybody to advise, what type automatic knife sharpener we can buy in Europe (Germany) instead Leica SP9000, which now is unavailable for sale? What vendors? We do not like a Shandon "Autosharp 5" type sharpener. Maxim Peshkov Russia, Taganrog. mailto:Maxim_71@mail.ru From one_angel_secret <@t> yahoo.com Thu Apr 5 16:09:14 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Apr 5 16:09:24 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <004601cd1360$ab3492f0$019db8d0$@com> References: <004601cd1360$ab3492f0$019db8d0$@com> Message-ID: <99C64616-4274-4AD0-88D2-FA9955E8B97A@yahoo.com> Hmm I found a letter regarding this. mohscollege.org/president/AETNAletter I must say the time restraint seems short but I am not surprised they are wanting it. With today's reimbursement rates and the economy we are in ins companies want to insure they get the highest quality of service for their dollars. I'm a little surprised they specifically want CAP though but nit that much. CLIA has deemed CAP authority to guide in the area of quality accreditation. No need to panic though. Remember. Times are changing and prosperity favors the ones who act upon knowledge :) Kim D Sent from my iPhone. On Apr 5, 2012, at 3:16 PM, "Carol Torrence" wrote: > We have received notification from AETNA that they now require non-hospital > labs to be accredited by CLIA and CAP. The letter makes it obvious that by > making such a request that they are not aware that CLIA assigned deemed > status to CAP and CLIA is actually the gatekeeper. Secondly we are told to > be registered by May 1st and accredited by August 1st (which CAP says is > impossible) or we will have to send our lab to either Quest or Ameripath > which includes Dermpath Diagnostics division. It fails to mention that > there are other CAP accredited non hospital labs in our state. The Aetna > contact number is either 'mailbox full" or even after leaving a message, no > return call. Me thinks me smells a rat. > > > > If you are a non-hospital lab, have you heard of this? Does your > dematopathologist or pathologist know this is coming? I am interested in > your comments. > > > > Carol M. Torrence, HT(ASCP)CM > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Thu Apr 5 16:15:19 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Apr 5 16:15:26 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <99C64616-4274-4AD0-88D2-FA9955E8B97A@yahoo.com> References: <004601cd1360$ab3492f0$019db8d0$@com> <99C64616-4274-4AD0-88D2-FA9955E8B97A@yahoo.com> Message-ID: <4E5F7C48-C170-46A8-BF2E-630B8E46D743@yahoo.com> http://www.mohscollege.org/president/AETNAletter.pdf Sent from my iPhone On Apr 5, 2012, at 5:09 PM, Kim Donadio wrote: > Hmm I found a letter regarding this. > > mohscollege.org/president/AETNAletter > > I must say the time restraint seems short but I am not surprised they are wanting it. With today's reimbursement rates and the economy we are in ins companies want to insure they get the highest quality of service for their dollars. > I'm a little surprised they specifically want CAP though but nit that much. CLIA has deemed CAP authority to guide in the area of quality accreditation. > No need to panic though. Remember. Times are changing and prosperity favors the ones who act upon knowledge :) > Kim D > Sent from my iPhone. > > On Apr 5, 2012, at 3:16 PM, "Carol Torrence" wrote: > >> We have received notification from AETNA that they now require non-hospital >> labs to be accredited by CLIA and CAP. The letter makes it obvious that by >> making such a request that they are not aware that CLIA assigned deemed >> status to CAP and CLIA is actually the gatekeeper. Secondly we are told to >> be registered by May 1st and accredited by August 1st (which CAP says is >> impossible) or we will have to send our lab to either Quest or Ameripath >> which includes Dermpath Diagnostics division. It fails to mention that >> there are other CAP accredited non hospital labs in our state. The Aetna >> contact number is either 'mailbox full" or even after leaving a message, no >> return call. Me thinks me smells a rat. >> >> >> >> If you are a non-hospital lab, have you heard of this? Does your >> dematopathologist or pathologist know this is coming? I am interested in >> your comments. >> >> >> >> Carol M. Torrence, HT(ASCP)CM >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Thu Apr 5 17:23:12 2012 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu Apr 5 17:23:19 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <4E5F7C48-C170-46A8-BF2E-630B8E46D743@yahoo.com> References: <004601cd1360$ab3492f0$019db8d0$@com> <99C64616-4274-4AD0-88D2-FA9955E8B97A@yahoo.com>, <4E5F7C48-C170-46A8-BF2E-630B8E46D743@yahoo.com> Message-ID: <49C7A360-4105-47C8-B37E-51C655388292@yumaregional.org> I think this is just the beginning,, hold on to your pants Sent from my iPad On Apr 5, 2012, at 2:16 PM, "Kim Donadio" wrote: > > http://www.mohscollege.org/president/AETNAletter.pdf > > Sent from my iPhone > > On Apr 5, 2012, at 5:09 PM, Kim Donadio wrote: > >> Hmm I found a letter regarding this. >> >> mohscollege.org/president/AETNAletter >> >> I must say the time restraint seems short but I am not surprised they are wanting it. With today's reimbursement rates and the economy we are in ins companies want to insure they get the highest quality of service for their dollars. >> I'm a little surprised they specifically want CAP though but nit that much. CLIA has deemed CAP authority to guide in the area of quality accreditation. >> No need to panic though. Remember. Times are changing and prosperity favors the ones who act upon knowledge :) >> Kim D >> Sent from my iPhone. >> >> On Apr 5, 2012, at 3:16 PM, "Carol Torrence" wrote: >> >>> We have received notification from AETNA that they now require non-hospital >>> labs to be accredited by CLIA and CAP. The letter makes it obvious that by >>> making such a request that they are not aware that CLIA assigned deemed >>> status to CAP and CLIA is actually the gatekeeper. Secondly we are told to >>> be registered by May 1st and accredited by August 1st (which CAP says is >>> impossible) or we will have to send our lab to either Quest or Ameripath >>> which includes Dermpath Diagnostics division. It fails to mention that >>> there are other CAP accredited non hospital labs in our state. The Aetna >>> contact number is either 'mailbox full" or even after leaving a message, no >>> return call. Me thinks me smells a rat. >>> >>> >>> >>> If you are a non-hospital lab, have you heard of this? Does your >>> dematopathologist or pathologist know this is coming? I am interested in >>> your comments. >>> >>> >>> >>> Carol M. Torrence, HT(ASCP)CM >>> >>> >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From katelin09htl <@t> gmail.com Thu Apr 5 18:00:44 2012 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Thu Apr 5 18:00:51 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <004601cd1360$ab3492f0$019db8d0$@com> References: <004601cd1360$ab3492f0$019db8d0$@com> Message-ID: We also received this notice. We contacted our local CLIA office who had heard of it this week as well. We are a small lab, so we are not sure yet how this change will impact us. I'd also be curious to know what smaller, private labs are planning on doing. -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence wrote: > We have received notification from AETNA that they now require non-hospital > labs to be accredited by CLIA and CAP. The letter makes it obvious that by > making such a request that they are not aware that CLIA assigned deemed > status to CAP and CLIA is actually the gatekeeper. Secondly we are told to > be registered by May 1st and accredited by August 1st (which CAP says is > impossible) or we will have to send our lab to either Quest or Ameripath > which includes Dermpath Diagnostics division. It fails to mention that > there are other CAP accredited non hospital labs in our state. The Aetna > contact number is either 'mailbox full" or even after leaving a message, no > return call. Me thinks me smells a rat. > > > > If you are a non-hospital lab, have you heard of this? Does your > dematopathologist or pathologist know this is coming? I am interested in > your comments. > > > > Carol M. Torrence, HT(ASCP)CM > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From one_angel_secret <@t> yahoo.com Thu Apr 5 18:30:55 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Apr 5 18:31:03 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: References: <004601cd1360$ab3492f0$019db8d0$@com> Message-ID: <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com> My lab can pass any inspection I have no fear Bring it on utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s Sent from my iPhone On Apr 5, 2012, at 7:00 PM, Katelin Lester wrote: > We also received this notice. We contacted our local CLIA office who had > heard of it this week as well. We are a small lab, so we are not sure yet > how this change will impact us. I'd also be curious to know what smaller, > private labs are planning on doing. > -- > Katelin Lester, HTL > Gastroenterology Specialists of Oregon, P.C. > Pathology Laboratory > (971) 224-2408 > > On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence wrote: > >> We have received notification from AETNA that they now require non-hospital >> labs to be accredited by CLIA and CAP. The letter makes it obvious that by >> making such a request that they are not aware that CLIA assigned deemed >> status to CAP and CLIA is actually the gatekeeper. Secondly we are told to >> be registered by May 1st and accredited by August 1st (which CAP says is >> impossible) or we will have to send our lab to either Quest or Ameripath >> which includes Dermpath Diagnostics division. It fails to mention that >> there are other CAP accredited non hospital labs in our state. The Aetna >> contact number is either 'mailbox full" or even after leaving a message, no >> return call. Me thinks me smells a rat. >> >> >> >> If you are a non-hospital lab, have you heard of this? Does your >> dematopathologist or pathologist know this is coming? I am interested in >> your comments. >> >> >> >> Carol M. Torrence, HT(ASCP)CM >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.Hart <@t> bms.com Thu Apr 5 19:05:46 2012 From: Heather.Hart <@t> bms.com (Hart, Heather) Date: Thu Apr 5 19:07:03 2012 Subject: [Histonet] Microwave use in histo lab Message-ID: <55EA897EF9AC2342BD9322B86AC195B61331DFB16C@ushpwbmsmmp007.one.ads.bms.com> Hello everyone! I am currently taking a class titled Current Trends & Applications in Applied Science & Technology which is essentially a capstone course for my BSAST degree completion. This course requires a four part research paper on any technology of choice in the (students) related field. The topic I have chosen is Microwave Use in the Histology Lab. I am trying to gather information for the third module in which I need to address the topics listed below. I would appreciate any personal input or opinions about the topic per guidelines listed. Thank you for your help! Heather Hart, MLT (ASCP) Various Perspectives and Opinions Provide alternative perspectives from experts on technology Political implications and influences Public opinion on technology such as the media, consumers and community Your assessment of how effective the initial planning and risk assessment was to the implementation and usage of the technology ?You are encouraged to access other users and/or associated technologists in order to gain insight into various perspectives and fully understand the applications of the technology selected.? This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From carrie.schray <@t> gmail.com Thu Apr 5 19:28:11 2012 From: carrie.schray <@t> gmail.com (Carrie Schray) Date: Thu Apr 5 19:28:16 2012 Subject: [Histonet] Loss of Matrigel material during staining In-Reply-To: <000a01cd1363$f2df1a50$d89d4ef0$@edu> References: <000a01cd1363$f2df1a50$d89d4ef0$@edu> Message-ID: My experience with this is that the processing schedule is too long and probably too much heat. I had some skin that was grown in a well plate from human cells processed on a biopsy run, they cut fantastic then the gel rolled onto itself during staining. Once we reduced the processing schedule and took heat away on the processing reagents it was much better. And, try to use super sticky slides, such as those you would use with bone samples. Carrie L. Schray Univ. of Michigan Medical School Unit for Laboratory Animal Medicine Ann Arbor, Michigan On 4/5/12, Amy Porter wrote: > To all: I have a client that we are attempting to stain H & E / as well as > immuno on FFPE mouse skin with matrigel plugs. These things section like a > dream and then when we attempt to stain only the matrigel part of the sample > is falling off. Does anyone have any tricks with this stuff they would be > willing to share? We are using charged slides and cutting at 5-6 microns > per the client. Thanks - > > > > Amy S. Porter, HT(ASCP) QIHC > > Michigan State University > > Investigative HistoPathology Laboratory > > William S. Spielman, Ph.D. - Director > > Patricia K. Senagore, M.D. - Consulting Pathologist > > Department of Physiology / Human Pathology > > Biomedical Physical Sciences Building > > 567 Wilson Road - Room 2133 > > East Lansing, MI 48824-3320 > > Phone: 517-884-5026 > > Fax: 517-432-1368 > > portera@msu.edu > > www.humanpathology.msu.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lpwenk <@t> sbcglobal.net Fri Apr 6 04:32:38 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Apr 6 04:32:49 2012 Subject: [Histonet] Microwave use in histo lab In-Reply-To: <55EA897EF9AC2342BD9322B86AC195B61331DFB16C@ushpwbmsmmp007.one.ads.bms.com> References: <55EA897EF9AC2342BD9322B86AC195B61331DFB16C@ushpwbmsmmp007.one.ads.bms.com> Message-ID: <071284BB0D684F36AFDB91B7CD0F19C5@HP2010> There is the document from CLSI on "Microwave Device Used in Histology Laboratory", GP28-A. If your lab is CAP accredited, your organization might already be a member of CLSI (Clinical and Laboratory Standards Institute) www.clsi.org so you might already have the ability to download this. It's a consensus document from experts in the field, and was put together over a several year period at the NSH Symposium, with lots on input from NSH members. Then it was open to the public, and any other histotech (researcher, electrician, whoever) could comment on it. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 "The opinions expressed are mine, and do not reflect Beaumont Hospital." -----Original Message----- From: Hart, Heather Sent: Thursday, April 05, 2012 8:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave use in histo lab Hello everyone! I am currently taking a class titled Current Trends & Applications in Applied Science & Technology which is essentially a capstone course for my BSAST degree completion. This course requires a four part research paper on any technology of choice in the (students) related field. The topic I have chosen is Microwave Use in the Histology Lab. I am trying to gather information for the third module in which I need to address the topics listed below. I would appreciate any personal input or opinions about the topic per guidelines listed. Thank you for your help! Heather Hart, MLT (ASCP) Various Perspectives and Opinions Provide alternative perspectives from experts on technology Political implications and influences Public opinion on technology such as the media, consumers and community Your assessment of how effective the initial planning and risk assessment was to the implementation and usage of the technology ?You are encouraged to access other users and/or associated technologists in order to gain insight into various perspectives and fully understand the applications of the technology selected.? This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fbozkurt <@t> gmail.com Fri Apr 6 04:36:41 2012 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Fri Apr 6 04:36:46 2012 Subject: [Histonet] Microwave use in histo lab In-Reply-To: <55EA897EF9AC2342BD9322B86AC195B61331DFB16C@ushpwbmsmmp007.one.ads.bms.com> References: <55EA897EF9AC2342BD9322B86AC195B61331DFB16C@ushpwbmsmmp007.one.ads.bms.com> Message-ID: Hello, http://www.ebsciences.com/papers/microwave.htm I hope this helps.. On Fri, Apr 6, 2012 at 3:05 AM, Hart, Heather wrote: > Hello everyone! > > I am currently taking a class titled Current Trends & Applications in > Applied Science & Technology which is essentially a capstone course for my > BSAST degree completion. This course requires a four part research paper > on any technology of choice in the (students) related field. The topic I > have chosen is Microwave Use in the Histology Lab. I am trying to gather > information for the third module in which I need to address the topics > listed below. I would appreciate any personal input or opinions about the > topic per guidelines listed. > > Thank you for your help! > > Heather Hart, MLT (ASCP) > > > Various Perspectives and Opinions > Provide alternative perspectives from experts on technology > Political implications and influences > Public opinion on technology such as the media, consumers and > community > Your assessment of how effective the initial planning and risk > assessment was to the implementation and usage of the technology > > ?You are encouraged to access other users and/or associated > technologists in order to gain insight into various perspectives and fully > understand the applications of the technology selected.? > > This message (including any attachments) may contain confidential, > proprietary, privileged and/or private information. The information is > intended to be for the use of the individual or entity designated above. > If you are not the intended recipient of this message, please notify the > sender immediately, and delete the message and any attachments. Any > disclosure, reproduction, distribution or other use of this message or any > attachments by an individual or entity other than the intended recipient is > prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 From sbreckenridge <@t> caperegional.com Fri Apr 6 08:27:25 2012 From: sbreckenridge <@t> caperegional.com (Breckenridge, Sue) Date: Fri Apr 6 08:27:47 2012 Subject: [Histonet] Molecular Testing requests Message-ID: Hi all! Just wondered how other histology labs are handling this scenario. We've noticed with the increase in physician options for Molecular Testing that we are being inundated with requests from physicians for testing from various reference labs. CAP has specific requirements for reference lab testing and so far our policy is to accept request from physician after checking that reference lab meets accrediting agency criteria. Now that there are so many, it gets cumbersome (not to mention confusing) when trying to write procedures for the testing and educate staff. How are others handling this? Do you request all new reference labs must be submitted for approval to physician executive committee or just pathologist prior to sending any test requests? How are you managing send-out involvement? Do you bill the patient (when Medicare 14 day rule not involved), or does the reference lab? Would greatly appreciate hearing from you concerning any processes you follow or insights you might have. Thanks! Sue Breckenridge, Histology Supervisor Cape Regional Medical Center Cape May Court House, NJ Confidentiality Notice: This e-mail message, including any attachments, from Cape Regional Health System contains information which is CONFIDENTIAL AND/OR LEGALLY PRIVILEGED.The information is intended only for the use of the individual named above and may not be disseminated to any other party without Cape Regional Health System's written permission. If you are not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this e-mailed information is strictly prohibited. If you have received this e-mail in error, please notify us immediately by telephone at 609-463-2163 to arrange for the return of these documents to us without cost to you. From t.magee <@t> gipath.com Fri Apr 6 08:33:13 2012 From: t.magee <@t> gipath.com (Tiffeny Magee) Date: Fri Apr 6 08:33:28 2012 Subject: [Histonet] Lets talk forceps Message-ID: <5D59AF96B902456A8B5948EB08BACE79@PATHOLOGY> I would love to buy a top of the line pair of forceps. One in partial that is smallish for a woman's hand and most importantly doesn't stick to the tissue on the water bath when I'm separating my sections. So does anyone have histology HT forceps they highly recommend? Thanks Tiffeny Magee From jqb7 <@t> cdc.gov Fri Apr 6 08:43:53 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Fri Apr 6 08:44:02 2012 Subject: [Histonet] Lets talk forceps In-Reply-To: <5D59AF96B902456A8B5948EB08BACE79@PATHOLOGY> References: <5D59AF96B902456A8B5948EB08BACE79@PATHOLOGY> Message-ID: I really like these and I have small hands and have had carpal tunnel surgery. Surgipath ergonomic forceps now available through Leica: 38DI15585 38DI15590 These both have a 5 ?" handle. One is curved and the other straight. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tiffeny Magee Sent: Friday, April 06, 2012 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lets talk forceps I would love to buy a top of the line pair of forceps. One in partial that is smallish for a woman's hand and most importantly doesn't stick to the tissue on the water bath when I'm separating my sections. So does anyone have histology HT forceps they highly recommend? Thanks Tiffeny Magee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From khairedai <@t> yahoo.com Fri Apr 6 08:46:08 2012 From: khairedai <@t> yahoo.com (Khaire Dai) Date: Fri Apr 6 08:46:12 2012 Subject: [Histonet] (no subject) Message-ID: <1333719968.38476.YahooMailMobile@web113004.mail.gq1.yahoo.com> http://meganandtravis.com/wp-content/plugins/extended-comment-options/rmngl.html From mcpeleteiro <@t> gmail.com Fri Apr 6 10:34:54 2012 From: mcpeleteiro <@t> gmail.com (Maria Peleteiro) Date: Fri Apr 6 10:35:03 2012 Subject: [Histonet] Shandon Citadel 2000 - Paraffin bath Message-ID: To the histonet list members Does any one has available for sale a 240 V - AC paraffin bath for a Shandon Citadel 2000? I am buying, with urgence. Maria C. Peleteiro Mobile - 351 963068730 From Heather.Hart <@t> bms.com Fri Apr 6 12:59:41 2012 From: Heather.Hart <@t> bms.com (Hart, Heather) Date: Fri Apr 6 12:59:46 2012 Subject: [Histonet] Microwave use in histo lab In-Reply-To: References: <55EA897EF9AC2342BD9322B86AC195B61331DFB16C@ushpwbmsmmp007.one.ads.bms.com> Message-ID: <55EA897EF9AC2342BD9322B86AC195B6133200B533@ushpwbmsmmp007.one.ads.bms.com> Mehmet, Thank you for your response. I have been utilizing that resource for other portions of the paper. Specifically, I am looking for any opinions good or bad on the technology from actual users, past or present. Such as if it is preferred in some labs (hospital and/or research), or by some technologists for certain tasks, but not others. Perhaps it was used before and phased out due to difficulties, etc. Again, thank you! Heather Hart From: Mehmet Fatih BOZKURT Hello, http://www.ebsciences.com/papers/microwave.htm I hope this helps. Various Perspectives and Opinions Provide alternative perspectives from experts on technology Public opinion on technology such as the media, consumers and community "You are encouraged to access other users and/or associated technologists in order to gain insight into various perspectives and fully understand the applications of the technology selected." ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From rsrichmond <@t> gmail.com Fri Apr 6 13:08:37 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Fri Apr 6 13:08:40 2012 Subject: [Histonet] Re: Let's talk forceps Message-ID: Arrrgh! Tweezers. Pickups. Grumpy old doc notes that in his day (1960s) the word "forceps" meant obstetrical forceps and nothing else. I was taught how to apply forceps, though medical students weren't actually allowed to. My eldest daughter was delivered by the "Professor of Forceps" (a much beloved practicing obstetrician with ideas far ahead of his time) to a crowd of admiring medical students, with an axis traction bar forceps (don't ask!) in the greatest feat of obstetrical grandstanding I ever witnessed. I'm quite sure that nobody misses obstetrical forceps! The VBAC came into use not long after I graduated. Thanks for the tip about the ergonomic, uh, tweezers. Filed for future reference. Bob Richmond Samurai Pathologist Knoxville TN From SLB <@t> stowers.org Fri Apr 6 13:18:58 2012 From: SLB <@t> stowers.org (Beckham, Sharon) Date: Fri Apr 6 13:19:02 2012 Subject: [Histonet] Re: Let's talk forceps In-Reply-To: References: Message-ID: <2C40E43D1F7A56408C4463FD245DDDF99BC60C26@EXCHMB-02.stowers-institute.org> Bob, I am so glad that someone else feels the same way as I do. I'm not the only person of a certain age in this histo world. Everytime I hear the word forceps, I think about giving birth. Both my kids were pulled out with forceps back in the '70's. I HATE that word! In histology I use tweezers! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Friday, April 06, 2012 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Let's talk forceps Arrrgh! Tweezers. Pickups. Grumpy old doc notes that in his day (1960s) the word "forceps" meant obstetrical forceps and nothing else. I was taught how to apply forceps, though medical students weren't actually allowed to. My eldest daughter was delivered by the "Professor of Forceps" (a much beloved practicing obstetrician with ideas far ahead of his time) to a crowd of admiring medical students, with an axis traction bar forceps (don't ask!) in the greatest feat of obstetrical grandstanding I ever witnessed. I'm quite sure that nobody misses obstetrical forceps! The VBAC came into use not long after I graduated. Thanks for the tip about the ergonomic, uh, tweezers. Filed for future reference. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Fri Apr 6 13:39:37 2012 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri Apr 6 13:40:01 2012 Subject: [Histonet] Histobath Message-ID: We are desperately looking for a Histobath. I know they do not make them anymore but if someone has a old one they are not using or a company can get their hands on one, we would be eternally grateful. Our Lab Manager would prefer we do not us Liquid Nitrogen. We love the Histobaths we have now. Marilyn Weiss HT (ASCP) cm Kaiser Permanente Hospital San Diego, Ca Marilyn.A.Weiss@kp.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From cls71877 <@t> sbcglobal.net Fri Apr 6 13:45:15 2012 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Fri Apr 6 13:45:19 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: References: <004601cd1360$ab3492f0$019db8d0$@com> Message-ID: <1333737915.99591.YahooMailRC@web81204.mail.mud.yahoo.com> We are a small GI lab.? While we have not yet received this notice, we have heard the rumors and found a letter via Google.? We are State compliant as we cannot solicit other practices for work.? The idea we have to be CAP accredited seems a little much.? As stated by someone else, CLIA has granted the privilege of certifying labs to many other governing bodies.? i believe our State inspector is just as competent and demanding of a quality product for quality patient care as any CAP inspector would be.? Also, the time constraint is completely unreasonable.? We just passed our State inspection with no deficiencies last month and have no intentions of making any changes until otherwise forced!? In speaking with my director, I believe it is our plan to appeal and protest if we receive do receive this notice.? Thanks Cristi ________________________________ From: Katelin Lester To: Carol Torrence Cc: histonet@lists.utsouthwestern.edu Sent: Thu, April 5, 2012 4:00:44 PM Subject: Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs We also received this notice. We contacted our local CLIA office who had heard of it this week as well. We are a small lab, so we are not sure yet how this change will impact us. I'd also be curious to know what smaller, private labs are planning on doing. -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence wrote: > We have received notification from AETNA that they now require non-hospital > labs to be accredited by CLIA and CAP.? The letter makes it obvious that by > making such a request that they are not aware that CLIA assigned deemed > status to CAP and CLIA is actually the gatekeeper.? Secondly we are told to > be registered by May 1st and accredited by August 1st (which CAP says is > impossible) or we will have to send our lab to either Quest or Ameripath > which includes Dermpath Diagnostics division.? It fails to mention that > there are other CAP accredited non hospital labs in our state.? The Aetna > contact number is either 'mailbox full" or even after leaving a message, no > return call.? Me thinks me smells a rat. > > > > If you are a non-hospital lab, have you heard of this?? Does your > dematopathologist or pathologist know this is coming?? I am interested in > your comments. > > > > Carol M. Torrence, HT(ASCP)CM > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Fri Apr 6 13:51:34 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Fri Apr 6 13:52:18 2012 Subject: [Histonet] Histobath In-Reply-To: References: Message-ID: <090FA56107A969459F3941DDD5585C3A1162A19A@PHSX10MB10.partners.org> I googled Histobath and Shandon sells them, plus some other on-line companies. Check it out. Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn.A.Weiss@kp.org Sent: Friday, April 06, 2012 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histobath We are desperately looking for a Histobath. I know they do not make them anymore but if someone has a old one they are not using or a company can get their hands on one, we would be eternally grateful. Our Lab Manager would prefer we do not us Liquid Nitrogen. We love the Histobaths we have now. Marilyn Weiss HT (ASCP) cm Kaiser Permanente Hospital San Diego, Ca Marilyn.A.Weiss@kp.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From talulahgosh <@t> gmail.com Fri Apr 6 14:02:56 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Apr 6 14:02:59 2012 Subject: [Histonet] Lets talk forceps In-Reply-To: References: <5D59AF96B902456A8B5948EB08BACE79@PATHOLOGY> Message-ID: Roboz makes great forceps and they aren't expensive. I think Storz may have bought them out. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Fri, Apr 6, 2012 at 9:43 AM, Bartlett, Jeanine (CDC/OID/NCEZID) < jqb7@cdc.gov> wrote: > I really like these and I have small hands and have had carpal tunnel > surgery. > > Surgipath ergonomic forceps now available through Leica: > > 38DI15585 > 38DI15590 > > These both have a 5 ?" handle. One is curved and the other straight. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tiffeny Magee > Sent: Friday, April 06, 2012 9:33 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Lets talk forceps > > I would love to buy a top of the line pair of forceps. One in partial that > is smallish for a woman's hand and most importantly doesn't stick to the > tissue on the water bath when I'm separating my sections. So does anyone > have histology HT forceps they highly recommend? > > > > Thanks > > Tiffeny Magee > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mannis <@t> usgs.gov Fri Apr 6 15:34:47 2012 From: mannis <@t> usgs.gov (Mandy Annis) Date: Fri Apr 6 15:34:51 2012 Subject: [Histonet] stainer and coverslipper recommendations for periodic use Message-ID: Histonet colleagues, We are interested in purchasing an automatic stainer and glass coverslipper, however as only part of a large research facility our histology laboratory receives sporadic use. Depending on studies, our lab may have several weeks or months of intensive use generating thousands of slides followed by weeks or months of disuse. Given our unique work load, I am concerned that such periods of inactivity maybe hard on a stainer and coverslipper. Keeping that in mind, can anyone recommend a stainer/coverslipper that can withstand inactivity and or that can be easily put in hibernation mode until needed? The stainer would be used primarily for H &E. Vendors please feel free to contribute. Thank you in advance! Mandy ********************************************************************** Mandy L. Annis United States Geological Survey Columbia Environmental Research Center 4200 New Haven RD Columbia, MO 65201 Phone:(573)-441-2940 Fax:(573)-876-1896 e-mail:mannis@usgs.gov From ctorrence <@t> kmcpa.com Fri Apr 6 15:40:33 2012 From: ctorrence <@t> kmcpa.com (Carol Torrence) Date: Fri Apr 6 15:41:00 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com> References: <004601cd1360$ab3492f0$019db8d0$@com> <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com> Message-ID: <001901cd1435$83a5dd40$8af197c0$@com> I too have been through many CAP inspections in the past. Passing is not my concern - how about expense, prep time, time away to inspect a peer. We are a small private lab also so this a bit of a pain. There is no way that CAP will be able to accommodate the workload that will ensue if this becomes a trend. Which I think it will and there will be more insurance companies aligning themselves with the larger labs as "preferred". My fear is that local healthcare will be so undercut that it will become more difficult if not impossible for even hospital labs to compete. I will never be convinced that big is better. I believe Aetna will hear from CAP on this issue due to the increased workload to them...deadlines may have to be extended. We are hearing from a CAP member that they will not be able to be accredited in such a short time, according to CAP. -----Original Message----- From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Thursday, April 05, 2012 6:31 PM To: Katelin Lester Cc: Carol Torrence; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs My lab can pass any inspection I have no fear Bring it on utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s Sent from my iPhone On Apr 5, 2012, at 7:00 PM, Katelin Lester wrote: > We also received this notice. We contacted our local CLIA office who > had heard of it this week as well. We are a small lab, so we are not > sure yet how this change will impact us. I'd also be curious to know > what smaller, private labs are planning on doing. > -- > Katelin Lester, HTL > Gastroenterology Specialists of Oregon, P.C. > Pathology Laboratory > (971) 224-2408 > > On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence wrote: > >> We have received notification from AETNA that they now require >> non-hospital labs to be accredited by CLIA and CAP. The letter makes >> it obvious that by making such a request that they are not aware that >> CLIA assigned deemed status to CAP and CLIA is actually the >> gatekeeper. Secondly we are told to be registered by May 1st and >> accredited by August 1st (which CAP says is >> impossible) or we will have to send our lab to either Quest or >> Ameripath which includes Dermpath Diagnostics division. It fails to >> mention that there are other CAP accredited non hospital labs in our >> state. The Aetna contact number is either 'mailbox full" or even >> after leaving a message, no return call. Me thinks me smells a rat. >> >> >> >> If you are a non-hospital lab, have you heard of this? Does your >> dematopathologist or pathologist know this is coming? I am >> interested in your comments. >> >> >> >> Carol M. Torrence, HT(ASCP)CM >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Fri Apr 6 15:51:12 2012 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Apr 6 15:51:19 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <001901cd1435$83a5dd40$8af197c0$@com> References: <004601cd1360$ab3492f0$019db8d0$@com> <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com>, <001901cd1435$83a5dd40$8af197c0$@com> Message-ID: <015703AD-C13F-4AF3-9C3C-2E733FCB4729@yumaregional.org> There are several frame of minds here, but most closely this aligns with the affordable care act and quality outcomes for patients. I to agree with the statement that other agencies can provide good quality outcomes, but Anatomic pathology is changing so rapidly. From all aspects, but if you look at who bills for most of the CMS testing it falls under hospital based laboratories, yet the government decides reimbursement based on what the large labs make.. In the end we are seeing consolidation,, but I hope someone comes to the forefront to speak for us all. Sent from my iPad On Apr 6, 2012, at 1:41 PM, "Carol Torrence" wrote: > I too have been through many CAP inspections in the past. Passing is not my > concern - how about expense, prep time, time away to inspect a peer. We > are a small private lab also so this a bit of a pain. There is no way that > CAP will be able to accommodate the workload that will ensue if this becomes > a trend. Which I think it will and there will be more insurance companies > aligning themselves with the larger labs as "preferred". My fear is that > local healthcare will be so undercut that it will become more difficult if > not impossible for even hospital labs to compete. I will never be convinced > that big is better. > > I believe Aetna will hear from CAP on this issue due to the increased > workload to them...deadlines may have to be extended. We are hearing from a > CAP member that they will not be able to be accredited in such a short time, > according to CAP. > > -----Original Message----- > From: Kim Donadio [mailto:one_angel_secret@yahoo.com] > Sent: Thursday, April 05, 2012 6:31 PM > To: Katelin Lester > Cc: Carol Torrence; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Aetna requiring CAP accreditation for non-hospital > labs > > My lab can pass any inspection I have no fear Bring it on > utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s > > Sent from my iPhone > > On Apr 5, 2012, at 7:00 PM, Katelin Lester wrote: > >> We also received this notice. We contacted our local CLIA office who >> had heard of it this week as well. We are a small lab, so we are not >> sure yet how this change will impact us. I'd also be curious to know >> what smaller, private labs are planning on doing. >> -- >> Katelin Lester, HTL >> Gastroenterology Specialists of Oregon, P.C. >> Pathology Laboratory >> (971) 224-2408 >> >> On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence > wrote: >> >>> We have received notification from AETNA that they now require >>> non-hospital labs to be accredited by CLIA and CAP. The letter makes >>> it obvious that by making such a request that they are not aware that >>> CLIA assigned deemed status to CAP and CLIA is actually the >>> gatekeeper. Secondly we are told to be registered by May 1st and >>> accredited by August 1st (which CAP says is >>> impossible) or we will have to send our lab to either Quest or >>> Ameripath which includes Dermpath Diagnostics division. It fails to >>> mention that there are other CAP accredited non hospital labs in our >>> state. The Aetna contact number is either 'mailbox full" or even >>> after leaving a message, no return call. Me thinks me smells a rat. >>> >>> >>> >>> If you are a non-hospital lab, have you heard of this? Does your >>> dematopathologist or pathologist know this is coming? I am >>> interested in your comments. >>> >>> >>> >>> Carol M. Torrence, HT(ASCP)CM >>> >>> >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From one_angel_secret <@t> yahoo.com Fri Apr 6 16:56:16 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Apr 6 16:56:21 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <001901cd1435$83a5dd40$8af197c0$@com> References: <004601cd1360$ab3492f0$019db8d0$@com> <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com> <001901cd1435$83a5dd40$8af197c0$@com> Message-ID: I agree it's to short of a time frame. I wonder if it has some to do with the affordable care act ? http://publications.milliman.com/publications/healthreform/pdfs/rating-underwriting-under-new.pdf Sent from my iPhone On Apr 6, 2012, at 4:40 PM, "Carol Torrence" wrote: > I too have been through many CAP inspections in the past. Passing is not my > concern - how about expense, prep time, time away to inspect a peer. We > are a small private lab also so this a bit of a pain. There is no way that > CAP will be able to accommodate the workload that will ensue if this becomes > a trend. Which I think it will and there will be more insurance companies > aligning themselves with the larger labs as "preferred". My fear is that > local healthcare will be so undercut that it will become more difficult if > not impossible for even hospital labs to compete. I will never be convinced > that big is better. > > I believe Aetna will hear from CAP on this issue due to the increased > workload to them...deadlines may have to be extended. We are hearing from a > CAP member that they will not be able to be accredited in such a short time, > according to CAP. > > -----Original Message----- > From: Kim Donadio [mailto:one_angel_secret@yahoo.com] > Sent: Thursday, April 05, 2012 6:31 PM > To: Katelin Lester > Cc: Carol Torrence; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Aetna requiring CAP accreditation for non-hospital > labs > > My lab can pass any inspection I have no fear Bring it on > utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s > > Sent from my iPhone > > On Apr 5, 2012, at 7:00 PM, Katelin Lester wrote: > >> We also received this notice. We contacted our local CLIA office who >> had heard of it this week as well. We are a small lab, so we are not >> sure yet how this change will impact us. I'd also be curious to know >> what smaller, private labs are planning on doing. >> -- >> Katelin Lester, HTL >> Gastroenterology Specialists of Oregon, P.C. >> Pathology Laboratory >> (971) 224-2408 >> >> On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence > wrote: >> >>> We have received notification from AETNA that they now require >>> non-hospital labs to be accredited by CLIA and CAP. The letter makes >>> it obvious that by making such a request that they are not aware that >>> CLIA assigned deemed status to CAP and CLIA is actually the >>> gatekeeper. Secondly we are told to be registered by May 1st and >>> accredited by August 1st (which CAP says is >>> impossible) or we will have to send our lab to either Quest or >>> Ameripath which includes Dermpath Diagnostics division. It fails to >>> mention that there are other CAP accredited non hospital labs in our >>> state. The Aetna contact number is either 'mailbox full" or even >>> after leaving a message, no return call. Me thinks me smells a rat. >>> >>> >>> >>> If you are a non-hospital lab, have you heard of this? Does your >>> dematopathologist or pathologist know this is coming? I am >>> interested in your comments. >>> >>> >>> >>> Carol M. Torrence, HT(ASCP)CM >>> >>> >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From one_angel_secret <@t> yahoo.com Fri Apr 6 16:56:58 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Apr 6 16:57:08 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <015703AD-C13F-4AF3-9C3C-2E733FCB4729@yumaregional.org> References: <004601cd1360$ab3492f0$019db8d0$@com> <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com> <001901cd1435$83a5dd40$8af197c0$@com> <015703AD-C13F-4AF3-9C3C-2E733FCB4729@yumaregional.org> Message-ID: <1B804D2F-EE38-45BF-B4A5-87BD424061CA@yahoo.com> Yikes I just 2 sec ago said that lol Sent from my iPhone On Apr 6, 2012, at 4:51 PM, Jesus Ellin wrote: > There are several frame of minds here, but most closely this aligns with the affordable care act and quality outcomes for patients. I to agree with the statement that other agencies can provide good quality outcomes, but Anatomic pathology is changing so rapidly. From all aspects, but if you look at who bills for most of the CMS testing it falls under hospital based laboratories, yet the government decides reimbursement based on what the large labs make.. In the end we are seeing consolidation,, but I hope someone comes to the forefront to speak for us all. > Sent from my iPad > On Apr 6, 2012, at 1:41 PM, "Carol Torrence" wrote: > >> I too have been through many CAP inspections in the past. Passing is not my >> concern - how about expense, prep time, time away to inspect a peer. We >> are a small private lab also so this a bit of a pain. There is no way that >> CAP will be able to accommodate the workload that will ensue if this becomes >> a trend. Which I think it will and there will be more insurance companies >> aligning themselves with the larger labs as "preferred". My fear is that >> local healthcare will be so undercut that it will become more difficult if >> not impossible for even hospital labs to compete. I will never be convinced >> that big is better. >> >> I believe Aetna will hear from CAP on this issue due to the increased >> workload to them...deadlines may have to be extended. We are hearing from a >> CAP member that they will not be able to be accredited in such a short time, >> according to CAP. >> >> -----Original Message----- >> From: Kim Donadio [mailto:one_angel_secret@yahoo.com] >> Sent: Thursday, April 05, 2012 6:31 PM >> To: Katelin Lester >> Cc: Carol Torrence; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Aetna requiring CAP accreditation for non-hospital >> labs >> >> My lab can pass any inspection I have no fear Bring it on >> utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s >> >> Sent from my iPhone >> >> On Apr 5, 2012, at 7:00 PM, Katelin Lester wrote: >> >>> We also received this notice. We contacted our local CLIA office who >>> had heard of it this week as well. We are a small lab, so we are not >>> sure yet how this change will impact us. I'd also be curious to know >>> what smaller, private labs are planning on doing. >>> -- >>> Katelin Lester, HTL >>> Gastroenterology Specialists of Oregon, P.C. >>> Pathology Laboratory >>> (971) 224-2408 >>> >>> On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence >> wrote: >>> >>>> We have received notification from AETNA that they now require >>>> non-hospital labs to be accredited by CLIA and CAP. The letter makes >>>> it obvious that by making such a request that they are not aware that >>>> CLIA assigned deemed status to CAP and CLIA is actually the >>>> gatekeeper. Secondly we are told to be registered by May 1st and >>>> accredited by August 1st (which CAP says is >>>> impossible) or we will have to send our lab to either Quest or >>>> Ameripath which includes Dermpath Diagnostics division. It fails to >>>> mention that there are other CAP accredited non hospital labs in our >>>> state. The Aetna contact number is either 'mailbox full" or even >>>> after leaving a message, no return call. Me thinks me smells a rat. >>>> >>>> >>>> >>>> If you are a non-hospital lab, have you heard of this? Does your >>>> dematopathologist or pathologist know this is coming? I am >>>> interested in your comments. >>>> >>>> >>>> >>>> Carol M. Torrence, HT(ASCP)CM >>>> >>>> >>>> >>>> >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ From pathlocums <@t> gmail.com Fri Apr 6 17:20:43 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Fri Apr 6 17:20:47 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <1B804D2F-EE38-45BF-B4A5-87BD424061CA@yahoo.com> References: <004601cd1360$ab3492f0$019db8d0$@com> <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com> <001901cd1435$83a5dd40$8af197c0$@com> <015703AD-C13F-4AF3-9C3C-2E733FCB4729@yumaregional.org> <1B804D2F-EE38-45BF-B4A5-87BD424061CA@yahoo.com> Message-ID: This is a fascinating thread!! So what are your thoughts on this - It would appear that, if other insurers follow suit, this could pose a huge burden on dermatologists that do their own tissue processing, and all the GI labs across the country that are popping up doing their own in-house histology. It may be hard, and in some cases not possible for those labs to become CAP accredited. In my opinion, that would be a great thing, to see all those physician offices doing histology close their lab doors, and focus on thier own specialty rather than invade the pathology world from which they were not trained. It would seem, to the average witness, that these facilities are treading on very thin ice as it is. It certainly does appear to be a violation of Stark laws that were created for a very good reason. In addition, these offices have stolen the "bread and butter" from large labs, and hospital pathology departments and left behind the far less profitable work. It would be nice to see that work return to the place it belongs - in Pathology laboratories. It may be a pipe dream at this point, but who knows - maybe this is the start of a very, very good thing. On Fri, Apr 6, 2012 at 2:56 PM, Kim Donadio wrote: > Yikes I just 2 sec ago said that lol > > Sent from my iPhone > > On Apr 6, 2012, at 4:51 PM, Jesus Ellin wrote: > > > There are several frame of minds here, but most closely this aligns with > the affordable care act and quality outcomes for patients. I to agree with > the statement that other agencies can provide good quality outcomes, but > Anatomic pathology is changing so rapidly. From all aspects, but if you > look at who bills for most of the CMS testing it falls under hospital based > laboratories, yet the government decides reimbursement based on what the > large labs make.. In the end we are seeing consolidation,, but I hope > someone comes to the forefront to speak for us all. > > Sent from my iPad > > On Apr 6, 2012, at 1:41 PM, "Carol Torrence" > wrote: > > > >> I too have been through many CAP inspections in the past. Passing is > not my > >> concern - how about expense, prep time, time away to inspect a peer. > We > >> are a small private lab also so this a bit of a pain. There is no way > that > >> CAP will be able to accommodate the workload that will ensue if this > becomes > >> a trend. Which I think it will and there will be more insurance > companies > >> aligning themselves with the larger labs as "preferred". My fear is > that > >> local healthcare will be so undercut that it will become more difficult > if > >> not impossible for even hospital labs to compete. I will never be > convinced > >> that big is better. > >> > >> I believe Aetna will hear from CAP on this issue due to the increased > >> workload to them...deadlines may have to be extended. We are hearing > from a > >> CAP member that they will not be able to be accredited in such a short > time, > >> according to CAP. > >> > >> -----Original Message----- > >> From: Kim Donadio [mailto:one_angel_secret@yahoo.com] > >> Sent: Thursday, April 05, 2012 6:31 PM > >> To: Katelin Lester > >> Cc: Carol Torrence; histonet@lists.utsouthwestern.edu > >> Subject: Re: [Histonet] Aetna requiring CAP accreditation for > non-hospital > >> labs > >> > >> My lab can pass any inspection I have no fear Bring it on > >> utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s > >> > >> Sent from my iPhone > >> > >> On Apr 5, 2012, at 7:00 PM, Katelin Lester > wrote: > >> > >>> We also received this notice. We contacted our local CLIA office who > >>> had heard of it this week as well. We are a small lab, so we are not > >>> sure yet how this change will impact us. I'd also be curious to know > >>> what smaller, private labs are planning on doing. > >>> -- > >>> Katelin Lester, HTL > >>> Gastroenterology Specialists of Oregon, P.C. > >>> Pathology Laboratory > >>> (971) 224-2408 > >>> > >>> On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence > >> wrote: > >>> > >>>> We have received notification from AETNA that they now require > >>>> non-hospital labs to be accredited by CLIA and CAP. The letter makes > >>>> it obvious that by making such a request that they are not aware that > >>>> CLIA assigned deemed status to CAP and CLIA is actually the > >>>> gatekeeper. Secondly we are told to be registered by May 1st and > >>>> accredited by August 1st (which CAP says is > >>>> impossible) or we will have to send our lab to either Quest or > >>>> Ameripath which includes Dermpath Diagnostics division. It fails to > >>>> mention that there are other CAP accredited non hospital labs in our > >>>> state. The Aetna contact number is either 'mailbox full" or even > >>>> after leaving a message, no return call. Me thinks me smells a rat. > >>>> > >>>> > >>>> > >>>> If you are a non-hospital lab, have you heard of this? Does your > >>>> dematopathologist or pathologist know this is coming? I am > >>>> interested in your comments. > >>>> > >>>> > >>>> > >>>> Carol M. Torrence, HT(ASCP)CM > >>>> > >>>> > >>>> > >>>> > >>>> > >>>> _______________________________________________ > >>>> Histonet mailing list > >>>> Histonet@lists.utsouthwestern.edu > >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>>> > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ______________________________________________________________________ > > This message is confidential, intended only for the named > > recipient(s) and may contain information that is privileged > > or exempt from disclosure under applicable law. If you are > > not the intended recipient(s), you are notified that the > > dissemination, distribution, or copying of this message is > > strictly prohibited. If you receive this message in error, > > or are not the named recipient(s), please notify the sender > > at either the e-mail, fax, address, or telephone number > > listed above and delete this e-mail from your computer. > > Thank You. > > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From wdesalvo.cac <@t> hotmail.com Fri Apr 6 22:57:58 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Fri Apr 6 22:58:03 2012 Subject: [Histonet] Histobath In-Reply-To: References: Message-ID: The Histobath has gone back into production, but we switched the the Hacker Bright Clini-RF freezer (http://www.hackerinstruments.com) and love the compact instrument that provides better control of temperature (-80 C.) w/ a work space inside the unit (-30 C.). We also use 3M Novec HFE-7100 fluid to freeze our tissues. William DeSalvo, B.S., HTL(ASCP) > To: histonet@lists.utsouthwestern.edu > From: Marilyn.A.Weiss@kp.org > Date: Fri, 6 Apr 2012 11:39:37 -0700 > Subject: [Histonet] Histobath > > We are desperately looking for a Histobath. I know they do not make them > anymore but if someone has a old one they are not using or a company can > get their hands on one, we would be eternally grateful. Our Lab Manager > would prefer we do not us Liquid Nitrogen. We love the Histobaths we have > now. > Marilyn Weiss HT (ASCP) cm > Kaiser Permanente Hospital > San Diego, Ca > Marilyn.A.Weiss@kp.org > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > e-mail, you are prohibited from sharing, copying, or otherwise using or > disclosing its contents. If you have received this e-mail in error, > please notify the sender immediately by reply e-mail and permanently > delete this e-mail and any attachments without reading, forwarding or > saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Apr 7 17:27:32 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Apr 7 17:27:37 2012 Subject: [Histonet] Re: Histobath Message-ID: Marilyn Weiss HT (ASCP) cm, Kaiser Permanente Hospital, San Diego, CA asks: >>We are desperately looking for a Histobath. I know they do not make them any more, but if someone has a old one they are not using or a company can get their hands on one, we would be eternally grateful. Our Lab Manager would prefer we do not us Liquid Nitrogen. We love the Histobaths we have now.<< After Googling the topic, I'm not at all convinced that the product is actually available. This topic has been discussed extensively on HistoNet in the last few years, and you should consult the HistoSearch archives. There is at least one alternative product. Whatever you get, look into using a non-flammable perfluorocarbon coolant, rather than the extremely flammable acetone or 2-methylbutane usually in use. Bob Richmond Samurai Pathologist Knoxville TN From Gary_Steinke <@t> vwr.com Sun Apr 8 13:02:59 2012 From: Gary_Steinke <@t> vwr.com (Gary_Steinke@vwr.com) Date: Sun Apr 8 13:03:04 2012 Subject: [Histonet] Gary Steinke is out of the office Message-ID: I will be out of the office starting 04/06/2012 and will not return until 04/11/2012. I will be unavailable until at least April 11th due to a family emergency. If you need immediate help, please call our Healthcare Customer Care Group at 877-881-1192 or by email at HEALTHCARESERVICE@VWR.COM. Thank you. From pruegg <@t> ihctech.net Sun Apr 8 14:15:07 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Apr 8 14:15:11 2012 Subject: [Histonet] Lets talk forceps In-Reply-To: References: <5D59AF96B902456A8B5948EB08BACE79@PATHOLOGY> Message-ID: <0E4FA7061FCA498F90623F509F5554C0@prueggihctechlt> I get my forceps, even the fine bent ones at Hobby Lobby in the jewelry making section, they are much cheaper than the medical supply companies and in my experience just as good. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, April 06, 2012 1:03 PM To: Bartlett, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Lets talk forceps Roboz makes great forceps and they aren't expensive. I think Storz may have bought them out. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson On Fri, Apr 6, 2012 at 9:43 AM, Bartlett, Jeanine (CDC/OID/NCEZID) < jqb7@cdc.gov> wrote: > I really like these and I have small hands and have had carpal tunnel > surgery. > > Surgipath ergonomic forceps now available through Leica: > > 38DI15585 > 38DI15590 > > These both have a 5 ?" handle. One is curved and the other straight. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tiffeny Magee > Sent: Friday, April 06, 2012 9:33 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Lets talk forceps > > I would love to buy a top of the line pair of forceps. One in partial that > is smallish for a woman's hand and most importantly doesn't stick to the > tissue on the water bath when I'm separating my sections. So does anyone > have histology HT forceps they highly recommend? > > > > Thanks > > Tiffeny Magee > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Apr 8 14:15:59 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Apr 8 14:16:03 2012 Subject: [Histonet] Histobath In-Reply-To: <090FA56107A969459F3941DDD5585C3A1162A19A@PHSX10MB10.partners.org> References: <090FA56107A969459F3941DDD5585C3A1162A19A@PHSX10MB10.partners.org> Message-ID: <47CACF30CBBE49049C2E1D4DB48EE7B6@prueggihctechlt> Is Shandon still around, I never see them anymore? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Friday, April 06, 2012 12:52 PM To: 'Marilyn.A.Weiss@kp.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histobath I googled Histobath and Shandon sells them, plus some other on-line companies. Check it out. Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn.A.Weiss@kp.org Sent: Friday, April 06, 2012 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histobath We are desperately looking for a Histobath. I know they do not make them anymore but if someone has a old one they are not using or a company can get their hands on one, we would be eternally grateful. Our Lab Manager would prefer we do not us Liquid Nitrogen. We love the Histobaths we have now. Marilyn Weiss HT (ASCP) cm Kaiser Permanente Hospital San Diego, Ca Marilyn.A.Weiss@kp.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Apr 8 14:31:38 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Apr 8 14:31:43 2012 Subject: [Histonet] Re: "placenta encapsulation" In-Reply-To: References: Message-ID: I took my granddaughters placenta at Beaumont Hospital in MI, we just told her OB/GYN in advance and he had a specimen jar of formalin in the delivery room, He cut off a piece of the placenta and put it in the specimen jar for us, handed it to me and I took it home. We use it as our placenta control tissue in the lab now. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Thursday, March 29, 2012 2:39 PM To: histonet@lists.utsouthwestern.edu; cmiller@gladstone.ucsf.edu Subject: [Histonet] Re: "placenta encapsulation" Hi, I would recommend getting someone else to do it for you. Unless you are *really* resilient, I would expect you would have other recovery related things to think about. With the OB/GYN's permission (and curiosity) I took my wife's placenta twice. Once for each kid. The local college was grateful to have it since it is a great tissue to learn on, and I also had an abundance of control tissue. The shock on my mother-in-law's face was palpable. She still thinks I'm nuts. Congratulations & Good Luck, Amos On Wed, Mar 28, 2012 at 10:10 PM, wrote: > Message: 14 > Date: Wed, 28 Mar 2012 18:40:39 -0700 > From: Caroline Miller > Subject: [Histonet] Re: "placenta encapsulation" > To: histonet@lists.utsouthwestern.edu > Message-ID: <8398DFB1-C338-4D6C-A63E-E76EE5DF83DC@gladstone.ucsf.edu> > Content-Type: text/plain; charset=us-ascii > > Well....I am 6 months pregnant, and was planning on taking a pot of > formalin and a few blades into the delivery suite to get some of my > placenta.......I am in real need of a good CD31 and CD34 control! > > We all give our bodies to this - right ;P > > Caroline > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Apr 8 14:36:04 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Apr 8 14:36:08 2012 Subject: [Histonet] Placenta Helper In-Reply-To: References: Message-ID: <77EBB2A754974C6F956ADB7430316B08@prueggihctechlt> Native Americans have eaten placenta and us old hippies started doing it back in the 60's, I missed that one myself though. I just read about a Hollywood actress who ate her placenta, makes sense, need to replenish all the blood and nutrients lost in giving birth, dogs do it. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Fisher Sent: Thursday, March 29, 2012 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Placenta Helper Placenta eaters have been around for a long time. In fact, many years ago on the original Saturday Night Live, Jane Curtin and Laraine Newman did an absolutely hilarious skit on Placenta Helper (Hamburger Helper). You can probably find it online. It's also been known that fresh placentas make great rose bush fertilizer and catfish bait! Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. B _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sye <@t> uic.edu Sun Apr 8 14:40:11 2012 From: sye <@t> uic.edu (Ye, Shuhua) Date: Sun Apr 8 14:40:36 2012 Subject: [Histonet] RE: [Please don't sent email to me any more I left this contry In-Reply-To: <0E4FA7061FCA498F90623F509F5554C0@prueggihctechlt> References: <5D59AF96B902456A8B5948EB08BACE79@PATHOLOGY> <0E4FA7061FCA498F90623F509F5554C0@prueggihctechlt> Message-ID: <761ce8358cf3e8b5e2cac428b6fcfd19.squirrel@webmail.uic.edu> Please don't sent email to me any more I left this contry On Sun, April 8, 2012 2:15 pm, Patsy Ruegg wrote: > I get my forceps, even the fine bent ones at Hobby Lobby in the jewelry > making section, they are much cheaper than the medical supply companies > and > in my experience just as good. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily > Sours > Sent: Friday, April 06, 2012 1:03 PM > To: Bartlett, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Lets talk forceps > > Roboz makes great forceps and they aren't expensive. I think Storz may > have bought them out. > > Emily > > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that?s beautiful. > --Ron Swanson > > > > On Fri, Apr 6, 2012 at 9:43 AM, Bartlett, Jeanine (CDC/OID/NCEZID) < > jqb7@cdc.gov> wrote: > >> I really like these and I have small hands and have had carpal tunnel >> surgery. >> >> Surgipath ergonomic forceps now available through Leica: >> >> 38DI15585 >> 38DI15590 >> >> These both have a 5 ?" handle. One is curved and the other straight. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tiffeny Magee >> Sent: Friday, April 06, 2012 9:33 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Lets talk forceps >> >> I would love to buy a top of the line pair of forceps. One in partial >> that >> is smallish for a woman's hand and most importantly doesn't stick to the >> tissue on the water bath when I'm separating my sections. So does anyone >> have histology HT forceps they highly recommend? >> >> >> >> Thanks >> >> Tiffeny Magee >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Shuhua Ye, M.D. University of Illinois at Chicago 820 S. Wood Street Urology Dept., M/C 955 Chicago, IL 60612 312-413-0397 phone 312-996-9649 fax sye@uic.edu From sye <@t> uic.edu Sun Apr 8 14:40:34 2012 From: sye <@t> uic.edu (Ye, Shuhua) Date: Sun Apr 8 14:40:39 2012 Subject: [Histonet] RE: [Please don't sent email to me any more I left this contry In-Reply-To: <0E4FA7061FCA498F90623F509F5554C0@prueggihctechlt> References: <5D59AF96B902456A8B5948EB08BACE79@PATHOLOGY> <0E4FA7061FCA498F90623F509F5554C0@prueggihctechlt> Message-ID: Please don't sent email to me any more I left this contry On Sun, April 8, 2012 2:15 pm, Patsy Ruegg wrote: > I get my forceps, even the fine bent ones at Hobby Lobby in the jewelry > making section, they are much cheaper than the medical supply companies > and > in my experience just as good. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily > Sours > Sent: Friday, April 06, 2012 1:03 PM > To: Bartlett, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Lets talk forceps > > Roboz makes great forceps and they aren't expensive. I think Storz may > have bought them out. > > Emily > > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that?s beautiful. > --Ron Swanson > > > > On Fri, Apr 6, 2012 at 9:43 AM, Bartlett, Jeanine (CDC/OID/NCEZID) < > jqb7@cdc.gov> wrote: > >> I really like these and I have small hands and have had carpal tunnel >> surgery. >> >> Surgipath ergonomic forceps now available through Leica: >> >> 38DI15585 >> 38DI15590 >> >> These both have a 5 ?" handle. One is curved and the other straight. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tiffeny Magee >> Sent: Friday, April 06, 2012 9:33 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Lets talk forceps >> >> I would love to buy a top of the line pair of forceps. One in partial >> that >> is smallish for a woman's hand and most importantly doesn't stick to the >> tissue on the water bath when I'm separating my sections. So does anyone >> have histology HT forceps they highly recommend? >> >> >> >> Thanks >> >> Tiffeny Magee >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Shuhua Ye, M.D. University of Illinois at Chicago 820 S. Wood Street Urology Dept., M/C 955 Chicago, IL 60612 312-413-0397 phone 312-996-9649 fax sye@uic.edu From sye <@t> uic.edu Sun Apr 8 14:40:35 2012 From: sye <@t> uic.edu (Ye, Shuhua) Date: Sun Apr 8 14:40:40 2012 Subject: [Histonet] RE: [Please don't sent email to me any more I left this contry In-Reply-To: <0E4FA7061FCA498F90623F509F5554C0@prueggihctechlt> References: <5D59AF96B902456A8B5948EB08BACE79@PATHOLOGY> <0E4FA7061FCA498F90623F509F5554C0@prueggihctechlt> Message-ID: <1b5b1fd7d2528049d39d5ddc4efd78e2.squirrel@webmail.uic.edu> Please don't sent email to me any more I left this contry On Sun, April 8, 2012 2:15 pm, Patsy Ruegg wrote: > I get my forceps, even the fine bent ones at Hobby Lobby in the jewelry > making section, they are much cheaper than the medical supply companies > and > in my experience just as good. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily > Sours > Sent: Friday, April 06, 2012 1:03 PM > To: Bartlett, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Lets talk forceps > > Roboz makes great forceps and they aren't expensive. I think Storz may > have bought them out. > > Emily > > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that?s beautiful. > --Ron Swanson > > > > On Fri, Apr 6, 2012 at 9:43 AM, Bartlett, Jeanine (CDC/OID/NCEZID) < > jqb7@cdc.gov> wrote: > >> I really like these and I have small hands and have had carpal tunnel >> surgery. >> >> Surgipath ergonomic forceps now available through Leica: >> >> 38DI15585 >> 38DI15590 >> >> These both have a 5 ?" handle. One is curved and the other straight. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tiffeny Magee >> Sent: Friday, April 06, 2012 9:33 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Lets talk forceps >> >> I would love to buy a top of the line pair of forceps. One in partial >> that >> is smallish for a woman's hand and most importantly doesn't stick to the >> tissue on the water bath when I'm separating my sections. So does anyone >> have histology HT forceps they highly recommend? >> >> >> >> Thanks >> >> Tiffeny Magee >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Shuhua Ye, M.D. University of Illinois at Chicago 820 S. Wood Street Urology Dept., M/C 955 Chicago, IL 60612 312-413-0397 phone 312-996-9649 fax sye@uic.edu From JMacDonald <@t> mtsac.edu Sun Apr 8 18:03:29 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun Apr 8 18:04:31 2012 Subject: [Histonet] Lets talk forceps In-Reply-To: <5D59AF96B902456A8B5948EB08BACE79@PATHOLOGY> Message-ID: http://www.statlab.com/product/stainless-steel-ergonomic-forceps "Tiffeny Magee" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/06/2012 06:33 AM To cc Subject [Histonet] Lets talk forceps I would love to buy a top of the line pair of forceps. One in partial that is smallish for a woman's hand and most importantly doesn't stick to the tissue on the water bath when I'm separating my sections. So does anyone have histology HT forceps they highly recommend? Thanks Tiffeny Magee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Mon Apr 9 05:33:35 2012 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Mon Apr 9 05:33:53 2012 Subject: [Histonet] Histobath In-Reply-To: <47CACF30CBBE49049C2E1D4DB48EE7B6@prueggihctechlt> References: <090FA56107A969459F3941DDD5585C3A1162A19A@PHSX10MB10.partners.org> <47CACF30CBBE49049C2E1D4DB48EE7B6@prueggihctechlt> Message-ID: I looked on line and never saw the Histobaths. They use to carry them a couple of years ago? I believe Shandon is a part of Thermo Fisher now. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. "Patsy Ruegg" 04/08/2012 12:15 PM To "'Sherwood, Margaret'" , Marilyn A Weiss/CA/KAIPERM@KAIPERM, cc Subject RE: [Histonet] Histobath Is Shandon still around, I never see them anymore? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Friday, April 06, 2012 12:52 PM To: 'Marilyn.A.Weiss@kp.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histobath I googled Histobath and Shandon sells them, plus some other on-line companies. Check it out. Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn.A.Weiss@kp.org Sent: Friday, April 06, 2012 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histobath We are desperately looking for a Histobath. I know they do not make them anymore but if someone has a old one they are not using or a company can get their hands on one, we would be eternally grateful. Our Lab Manager would prefer we do not us Liquid Nitrogen. We love the Histobaths we have now. Marilyn Weiss HT (ASCP) cm Kaiser Permanente Hospital San Diego, Ca Marilyn.A.Weiss@kp.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicole <@t> dlcjax.com Mon Apr 9 07:29:10 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Mon Apr 9 07:29:19 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: References: <004601cd1360$ab3492f0$019db8d0$@com> <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com> <001901cd1435$83a5dd40$8af197c0$@com> <015703AD-C13F-4AF3-9C3C-2E733FCB4729@yumaregional.org> <1B804D2F-EE38-45BF-B4A5-87BD424061CA@yahoo.com> Message-ID: <4988.208.62.167.196.1333974550.squirrel@webmail.realpages.com> Wow David, I strongly disagree with you. I happen to work and run at Dermatopathology lab and I am a licensed Histotechnologist. I have worked in the field for twelve years and I produce the same quality of work that I would if I was in a hospital. I also have to comply with the same state and CLIA rules you do. If not we woundlt be "treading on thim ice" we would be closed down. CLIA would not let my facility remain open if I was not producing the quality of work expected from all histology laboratories. Also, I cannt believe you would want our labs closed down. Do you know how many of YOUR FELLOW histologist you would put out of work. David is such a team player!!!! In reguards to stark laws. Maybe you should read what it actually means!!!! We only process specimens within our our facility and our slides ARE read by a licensed dermatopathologist. So, please tell me how my work is any less important than ur hospital job? And let me tell you this about my mediocker job. I work M-F 9-5. no weekends and no on-call. I also have full benefits and 401K. So, Im sorry that you feel our labs are some how underqualified, but I would not go back to hospital work, to save my life. Thanks for having my back, your fellow histologist. Nicole Tatum, HT ASCP This is a fascinating thread!! > > So what are your thoughts on this - It would appear that, if other > insurers > follow suit, this could pose a huge burden on dermatologists that do their > own tissue processing, and all the GI labs across the country that are > popping up doing their own in-house histology. It may be hard, and in some > cases not possible for those labs to become CAP accredited. > > In my opinion, that would be a great thing, to see all those physician > offices doing histology close their lab doors, and focus on thier own > specialty rather than invade the pathology world from which they were not > trained. It would seem, to the average witness, that these facilities are > treading on very thin ice as it is. It certainly does appear to be a > violation of Stark laws that were created for a very good reason. In > addition, these offices have stolen the "bread and butter" from large > labs, > and hospital pathology departments and left behind the far less profitable > work. It would be nice to see that work return to the place it belongs - > in > Pathology laboratories. > > It may be a pipe dream at this point, but who knows - maybe this is the > start of a very, very good thing. > > > > On Fri, Apr 6, 2012 at 2:56 PM, Kim Donadio > wrote: > >> Yikes I just 2 sec ago said that lol >> >> Sent from my iPhone >> >> On Apr 6, 2012, at 4:51 PM, Jesus Ellin wrote: >> >> > There are several frame of minds here, but most closely this aligns >> with >> the affordable care act and quality outcomes for patients. I to agree >> with >> the statement that other agencies can provide good quality outcomes, but >> Anatomic pathology is changing so rapidly. From all aspects, but if you >> look at who bills for most of the CMS testing it falls under hospital >> based >> laboratories, yet the government decides reimbursement based on what the >> large labs make.. In the end we are seeing consolidation,, but I hope >> someone comes to the forefront to speak for us all. >> > Sent from my iPad >> > On Apr 6, 2012, at 1:41 PM, "Carol Torrence" >> wrote: >> > >> >> I too have been through many CAP inspections in the past. Passing is >> not my >> >> concern - how about expense, prep time, time away to inspect a peer. >> We >> >> are a small private lab also so this a bit of a pain. There is no >> way >> that >> >> CAP will be able to accommodate the workload that will ensue if this >> becomes >> >> a trend. Which I think it will and there will be more insurance >> companies >> >> aligning themselves with the larger labs as "preferred". My fear is >> that >> >> local healthcare will be so undercut that it will become more >> difficult >> if >> >> not impossible for even hospital labs to compete. I will never be >> convinced >> >> that big is better. >> >> >> >> I believe Aetna will hear from CAP on this issue due to the increased >> >> workload to them...deadlines may have to be extended. We are hearing >> from a >> >> CAP member that they will not be able to be accredited in such a >> short >> time, >> >> according to CAP. >> >> >> >> -----Original Message----- >> >> From: Kim Donadio [mailto:one_angel_secret@yahoo.com] >> >> Sent: Thursday, April 05, 2012 6:31 PM >> >> To: Katelin Lester >> >> Cc: Carol Torrence; histonet@lists.utsouthwestern.edu >> >> Subject: Re: [Histonet] Aetna requiring CAP accreditation for >> non-hospital >> >> labs >> >> >> >> My lab can pass any inspection I have no fear Bring it on >> >> utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s >> >> >> >> Sent from my iPhone >> >> >> >> On Apr 5, 2012, at 7:00 PM, Katelin Lester >> wrote: >> >> >> >>> We also received this notice. We contacted our local CLIA office who >> >>> had heard of it this week as well. We are a small lab, so we are not >> >>> sure yet how this change will impact us. I'd also be curious to know >> >>> what smaller, private labs are planning on doing. >> >>> -- >> >>> Katelin Lester, HTL >> >>> Gastroenterology Specialists of Oregon, P.C. >> >>> Pathology Laboratory >> >>> (971) 224-2408 >> >>> >> >>> On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence >> >> >> wrote: >> >>> >> >>>> We have received notification from AETNA that they now require >> >>>> non-hospital labs to be accredited by CLIA and CAP. The letter >> makes >> >>>> it obvious that by making such a request that they are not aware >> that >> >>>> CLIA assigned deemed status to CAP and CLIA is actually the >> >>>> gatekeeper. Secondly we are told to be registered by May 1st and >> >>>> accredited by August 1st (which CAP says is >> >>>> impossible) or we will have to send our lab to either Quest or >> >>>> Ameripath which includes Dermpath Diagnostics division. It fails >> to >> >>>> mention that there are other CAP accredited non hospital labs in >> our >> >>>> state. The Aetna contact number is either 'mailbox full" or even >> >>>> after leaving a message, no return call. Me thinks me smells a >> rat. >> >>>> >> >>>> >> >>>> >> >>>> If you are a non-hospital lab, have you heard of this? Does your >> >>>> dematopathologist or pathologist know this is coming? I am >> >>>> interested in your comments. >> >>>> >> >>>> >> >>>> >> >>>> Carol M. Torrence, HT(ASCP)CM >> >>>> >> >>>> >> >>>> >> >>>> >> >>>> >> >>>> _______________________________________________ >> >>>> Histonet mailing list >> >>>> Histonet@lists.utsouthwestern.edu >> >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>>> >> >>> _______________________________________________ >> >>> Histonet mailing list >> >>> Histonet@lists.utsouthwestern.edu >> >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> >> >> >> >> >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > ______________________________________________________________________ >> > This message is confidential, intended only for the named >> > recipient(s) and may contain information that is privileged >> > or exempt from disclosure under applicable law. If you are >> > not the intended recipient(s), you are notified that the >> > dissemination, distribution, or copying of this message is >> > strictly prohibited. If you receive this message in error, >> > or are not the named recipient(s), please notify the sender >> > at either the e-mail, fax, address, or telephone number >> > listed above and delete this e-mail from your computer. >> > Thank You. >> > ______________________________________________________________________ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From b-frederick <@t> northwestern.edu Mon Apr 9 07:45:38 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Apr 9 07:45:44 2012 Subject: [Histonet] Histobath In-Reply-To: <47CACF30CBBE49049C2E1D4DB48EE7B6@prueggihctechlt> References: <090FA56107A969459F3941DDD5585C3A1162A19A@PHSX10MB10.partners.org> <47CACF30CBBE49049C2E1D4DB48EE7B6@prueggihctechlt> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E236E7D@evcspmbx3.ads.northwestern.edu> Fisher owns Shandon. Part of Thermo-fisher. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Sunday, April 08, 2012 2:16 PM To: 'Sherwood, Margaret'; Marilyn.A.Weiss@kp.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histobath Is Shandon still around, I never see them anymore? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Friday, April 06, 2012 12:52 PM To: 'Marilyn.A.Weiss@kp.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histobath I googled Histobath and Shandon sells them, plus some other on-line companies. Check it out. Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn.A.Weiss@kp.org Sent: Friday, April 06, 2012 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histobath We are desperately looking for a Histobath. I know they do not make them anymore but if someone has a old one they are not using or a company can get their hands on one, we would be eternally grateful. Our Lab Manager would prefer we do not us Liquid Nitrogen. We love the Histobaths we have now. Marilyn Weiss HT (ASCP) cm Kaiser Permanente Hospital San Diego, Ca Marilyn.A.Weiss@kp.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon Apr 9 07:54:42 2012 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Apr 9 07:54:46 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <4988.208.62.167.196.1333974550.squirrel@webmail.realpages.com> References: <004601cd1360$ab3492f0$019db8d0$@com> <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com> <001901cd1435$83a5dd40$8af197c0$@com> <015703AD-C13F-4AF3-9C3C-2E733FCB4729@yumaregional.org> <1B804D2F-EE38-45BF-B4A5-87BD424061CA@yahoo.com> <4988.208.62.167.196.1333974550.squirrel@webmail.realpages.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E236EA8@evcspmbx3.ads.northwestern.edu> I know many certified techs in independent labs and I know for a fact that at least two of them are CAP certified. I can see where an insurance company would want a lab that is doing work that will determine a patient treatment to be on the up and up. I work in cancer research and as the lab for ECOG (Eastern Cooperative Oncology Group)of which many of you submit blocks to for patients on clinical trials. We are in our CAP window right now (so the bosses are stressed). We are all registered techs. And our work can determine chemo arm, future treatments (archival blocks are used for new therapies). Don't mess with us techs not in a hospital. I did it for 20 years. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Monday, April 09, 2012 7:29 AM To: Davide Costanzo; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs Wow David, I strongly disagree with you. I happen to work and run at Dermatopathology lab and I am a licensed Histotechnologist. I have worked in the field for twelve years and I produce the same quality of work that I would if I was in a hospital. I also have to comply with the same state and CLIA rules you do. If not we woundlt be "treading on thim ice" we would be closed down. CLIA would not let my facility remain open if I was not producing the quality of work expected from all histology laboratories. Also, I cannt believe you would want our labs closed down. Do you know how many of YOUR FELLOW histologist you would put out of work. David is such a team player!!!! In reguards to stark laws. Maybe you should read what it actually means!!!! We only process specimens within our our facility and our slides ARE read by a licensed dermatopathologist. So, please tell me how my work is any less important than ur hospital job? And let me tell you this about my mediocker job. I work M-F 9-5. no weekends and no on-call. I also have full benefits and 401K. So, Im sorry that you feel our labs are some how underqualified, but I would not go back to hospital work, to save my life. Thanks for having my back, your fellow histologist. Nicole Tatum, HT ASCP This is a fascinating thread!! > > So what are your thoughts on this - It would appear that, if other > insurers follow suit, this could pose a huge burden on dermatologists > that do their own tissue processing, and all the GI labs across the > country that are popping up doing their own in-house histology. It may > be hard, and in some cases not possible for those labs to become CAP > accredited. > > In my opinion, that would be a great thing, to see all those physician > offices doing histology close their lab doors, and focus on thier own > specialty rather than invade the pathology world from which they were > not trained. It would seem, to the average witness, that these > facilities are treading on very thin ice as it is. It certainly does > appear to be a violation of Stark laws that were created for a very > good reason. In addition, these offices have stolen the "bread and > butter" from large labs, and hospital pathology departments and left > behind the far less profitable work. It would be nice to see that work > return to the place it belongs - in Pathology laboratories. > > It may be a pipe dream at this point, but who knows - maybe this is > the start of a very, very good thing. > > > > On Fri, Apr 6, 2012 at 2:56 PM, Kim Donadio > wrote: > >> Yikes I just 2 sec ago said that lol >> >> Sent from my iPhone >> >> On Apr 6, 2012, at 4:51 PM, Jesus Ellin wrote: >> >> > There are several frame of minds here, but most closely this aligns >> with >> the affordable care act and quality outcomes for patients. I to >> agree with the statement that other agencies can provide good quality >> outcomes, but Anatomic pathology is changing so rapidly. From all >> aspects, but if you look at who bills for most of the CMS testing it >> falls under hospital based laboratories, yet the government decides >> reimbursement based on what the large labs make.. In the end we are >> seeing consolidation,, but I hope someone comes to the forefront to >> speak for us all. >> > Sent from my iPad >> > On Apr 6, 2012, at 1:41 PM, "Carol Torrence" >> wrote: >> > >> >> I too have been through many CAP inspections in the past. Passing >> >> is >> not my >> >> concern - how about expense, prep time, time away to inspect a peer. >> We >> >> are a small private lab also so this a bit of a pain. There is no >> way >> that >> >> CAP will be able to accommodate the workload that will ensue if >> >> this >> becomes >> >> a trend. Which I think it will and there will be more insurance >> companies >> >> aligning themselves with the larger labs as "preferred". My fear >> >> is >> that >> >> local healthcare will be so undercut that it will become more >> difficult >> if >> >> not impossible for even hospital labs to compete. I will never be >> convinced >> >> that big is better. >> >> >> >> I believe Aetna will hear from CAP on this issue due to the >> >> increased workload to them...deadlines may have to be extended. >> >> We are hearing >> from a >> >> CAP member that they will not be able to be accredited in such a >> short >> time, >> >> according to CAP. >> >> >> >> -----Original Message----- >> >> From: Kim Donadio [mailto:one_angel_secret@yahoo.com] >> >> Sent: Thursday, April 05, 2012 6:31 PM >> >> To: Katelin Lester >> >> Cc: Carol Torrence; histonet@lists.utsouthwestern.edu >> >> Subject: Re: [Histonet] Aetna requiring CAP accreditation for >> non-hospital >> >> labs >> >> >> >> My lab can pass any inspection I have no fear Bring it on >> >> utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s >> >> >> >> Sent from my iPhone >> >> >> >> On Apr 5, 2012, at 7:00 PM, Katelin Lester >> >> >> wrote: >> >> >> >>> We also received this notice. We contacted our local CLIA office >> >>> who had heard of it this week as well. We are a small lab, so we >> >>> are not sure yet how this change will impact us. I'd also be >> >>> curious to know what smaller, private labs are planning on doing. >> >>> -- >> >>> Katelin Lester, HTL >> >>> Gastroenterology Specialists of Oregon, P.C. >> >>> Pathology Laboratory >> >>> (971) 224-2408 >> >>> >> >>> On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence >> >> >> wrote: >> >>> >> >>>> We have received notification from AETNA that they now require >> >>>> non-hospital labs to be accredited by CLIA and CAP. The letter >> makes >> >>>> it obvious that by making such a request that they are not aware >> that >> >>>> CLIA assigned deemed status to CAP and CLIA is actually the >> >>>> gatekeeper. Secondly we are told to be registered by May 1st >> >>>> and accredited by August 1st (which CAP says is >> >>>> impossible) or we will have to send our lab to either Quest or >> >>>> Ameripath which includes Dermpath Diagnostics division. It >> >>>> fails >> to >> >>>> mention that there are other CAP accredited non hospital labs in >> our >> >>>> state. The Aetna contact number is either 'mailbox full" or >> >>>> even after leaving a message, no return call. Me thinks me >> >>>> smells a >> rat. >> >>>> >> >>>> >> >>>> >> >>>> If you are a non-hospital lab, have you heard of this? Does >> >>>> your dematopathologist or pathologist know this is coming? I am >> >>>> interested in your comments. >> >>>> >> >>>> >> >>>> >> >>>> Carol M. Torrence, HT(ASCP)CM >> >>>> >> >>>> >> >>>> >> >>>> >> >>>> >> >>>> _______________________________________________ >> >>>> Histonet mailing list >> >>>> Histonet@lists.utsouthwestern.edu >> >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>>> >> >>> _______________________________________________ >> >>> Histonet mailing list >> >>> Histonet@lists.utsouthwestern.edu >> >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> >> >> >> >> >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > ___________________________________________________________________ >> > ___ This message is confidential, intended only for the named >> > recipient(s) and may contain information that is privileged or >> > exempt from disclosure under applicable law. If you are not the >> > intended recipient(s), you are notified that the dissemination, >> > distribution, or copying of this message is strictly prohibited. >> > If you receive this message in error, or are not the named >> > recipient(s), please notify the sender at either the e-mail, fax, >> > address, or telephone number listed above and delete this e-mail >> > from your computer. >> > Thank You. >> > ___________________________________________________________________ >> > ___ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Mon Apr 9 08:16:23 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon Apr 9 08:16:26 2012 Subject: [Histonet] HistoBath, HistoChill, Clini-RF Message-ID: Terri Bishop at SPScientific sent me an e-mail about HistoChill, a frozen section freezing bath that replaces the discontinued HistoBath. Terri didn't feel it was appropriate for a vendor to post this directly on HistoNet, so I am. You can contact Terri Bishop at Terri.Bishop@SPScientific.com HistoChill has been available for about a year. You can see the brochure at http://www.spscientific.com/Air-Stream-/-Baths-/-Chillers-/-Traps-/-Probes.aspx I'm pleased that they are specifically recommending using 3M's non-flammable perfluorocarbon HFE-7000 coolant, and not isopentane or acetone. (I feel like I've struck a blow for lab safety!) As has been noted on HistoNet before, Hacker Instruments offers Alan Bright's Clini-RF, a competing product. I have no commercial connection with any of the companies I've mentioned, and I have no personal experience with either instrument. Bob Richmond Samurai Pathologist Knoxville TN From contact <@t> excaliburpathology.com Mon Apr 9 08:19:45 2012 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Apr 9 08:19:52 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <4988.208.62.167.196.1333974550.squirrel@webmail.realpages.com> References: <004601cd1360$ab3492f0$019db8d0$@com> <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com> <001901cd1435$83a5dd40$8af197c0$@com> <015703AD-C13F-4AF3-9C3C-2E733FCB4729@yumaregional.org> <1B804D2F-EE38-45BF-B4A5-87BD424061CA@yahoo.com> <4988.208.62.167.196.1333974550.squirrel@webmail.realpages.com> Message-ID: <1333977585.56151.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> Bravo Nicole! ? Yet another pompous post from D. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Nicole Tatum To: Davide Costanzo ; histonet@lists.utsouthwestern.edu Sent: Monday, April 9, 2012 7:29 AM Subject: Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs Wow David, I strongly disagree with you. I happen to work and run at Dermatopathology lab and I am a licensed Histotechnologist. I have worked in the field for twelve years and I produce the same quality of work that I would if I was in a hospital. I also have to comply with the same state and CLIA rules you do. If not we woundlt be "treading on thim ice" we would be closed down. CLIA would not let my facility remain open if I was not producing the quality of work expected from all histology laboratories. Also, I cannt believe you would want our labs closed down. Do you know how many of YOUR FELLOW histologist you would put out of work. David is such a team player!!!! In reguards to stark laws. Maybe you should read what it actually means!!!! We only process specimens within our our facility and our slides ARE read by a licensed dermatopathologist. So, please tell me how my work is any less important than ur hospital job? And let me tell you this about my mediocker job. I work M-F 9-5. no weekends and no on-call. I also have full benefits and 401K. So, Im sorry that you feel our labs are some how underqualified, but I would not go back to hospital work, to save my life. Thanks for having my back, your fellow histologist. Nicole Tatum, HT ASCP This is a fascinating thread!! > > So what are your thoughts on this - It would appear that, if other > insurers > follow suit, this could pose a huge burden on dermatologists that do their > own tissue processing, and all the GI labs across the country that are > popping up doing their own in-house histology. It may be hard, and in some > cases not possible for those labs to become CAP accredited. > > In my opinion, that would be a great thing, to see all those physician > offices doing histology close their lab doors, and focus on thier own > specialty rather than invade the pathology world from which they were not > trained. It would seem, to the average witness, that these facilities are > treading on very thin ice as it is. It certainly does appear to be a > violation of Stark laws that were created for a very good reason. In > addition, these offices have stolen the "bread and butter" from large > labs, > and hospital pathology departments and left behind the far less profitable > work. It would be nice to see that work return to the place it belongs - > in > Pathology laboratories. > > It may be a pipe dream at this point, but who knows - maybe this is the > start of a very, very good thing. > > > > On Fri, Apr 6, 2012 at 2:56 PM, Kim Donadio > wrote: > >> Yikes I just 2 sec ago said that lol >> >> Sent from my iPhone >> >> On Apr 6, 2012, at 4:51 PM, Jesus Ellin wrote: >> >> > There are several frame of minds here, but most closely this aligns >> with >> the affordable care act and quality outcomes for patients.? I to agree >> with >> the statement that other agencies can provide good quality outcomes, but >> Anatomic pathology is changing so rapidly.? From all aspects, but if you >> look at who bills for most of the CMS testing it falls under hospital >> based >> laboratories, yet the government decides reimbursement based on what the >> large labs make..? In the end we are seeing consolidation,, but I hope >> someone comes to the forefront to speak for us all. >> > Sent from my iPad >> > On Apr 6, 2012, at 1:41 PM, "Carol Torrence" >> wrote: >> > >> >> I too have been through many CAP inspections in the past. Passing is >> not my >> >> concern - how about expense, prep time, time away to inspect a peer. >>? We >> >> are a small private lab also so this a bit of a pain.? There is no >> way >> that >> >> CAP will be able to accommodate the workload that will ensue if this >> becomes >> >> a trend. Which I think it will and there will be more insurance >> companies >> >> aligning themselves with the larger labs as "preferred".? My fear is >> that >> >> local healthcare will be so undercut that it will become more >> difficult >> if >> >> not impossible for even hospital labs to compete. I will never be >> convinced >> >> that big is better. >> >> >> >> I believe Aetna will hear from CAP on this issue due to the increased >> >> workload to them...deadlines may have to be extended.? We are hearing >> from a >> >> CAP member that they will not be able to be accredited in such a >> short >> time, >> >> according to CAP. >> >> >> >> -----Original Message----- >> >> From: Kim Donadio [mailto:one_angel_secret@yahoo.com] >> >> Sent: Thursday, April 05, 2012 6:31 PM >> >> To: Katelin Lester >> >> Cc: Carol Torrence; histonet@lists.utsouthwestern.edu >> >> Subject: Re: [Histonet] Aetna requiring CAP accreditation for >> non-hospital >> >> labs >> >> >> >> My lab can pass any inspection I have no fear Bring it on >> >> utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s >> >> >> >> Sent from my iPhone >> >> >> >> On Apr 5, 2012, at 7:00 PM, Katelin Lester >> wrote: >> >> >> >>> We also received this notice. We contacted our local CLIA office who >> >>> had heard of it this week as well. We are a small lab, so we are not >> >>> sure yet how this change will impact us. I'd also be curious to know >> >>> what smaller, private labs are planning on doing. >> >>> -- >> >>> Katelin Lester, HTL >> >>> Gastroenterology Specialists of Oregon, P.C. >> >>> Pathology Laboratory >> >>> (971) 224-2408 >> >>> >> >>> On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence >> >> >> wrote: >> >>> >> >>>> We have received notification from AETNA that they now require >> >>>> non-hospital labs to be accredited by CLIA and CAP.? The letter >> makes >> >>>> it obvious that by making such a request that they are not aware >> that >> >>>> CLIA assigned deemed status to CAP and CLIA is actually the >> >>>> gatekeeper.? Secondly we are told to be registered by May 1st and >> >>>> accredited by August 1st (which CAP says is >> >>>> impossible) or we will have to send our lab to either Quest or >> >>>> Ameripath which includes Dermpath Diagnostics division.? It fails >> to >> >>>> mention that there are other CAP accredited non hospital labs in >> our >> >>>> state.? The Aetna contact number is either 'mailbox full" or even >> >>>> after leaving a message, no return call.? Me thinks me smells a >> rat. >> >>>> >> >>>> >> >>>> >> >>>> If you are a non-hospital lab, have you heard of this?? Does your >> >>>> dematopathologist or pathologist know this is coming?? I am >> >>>> interested in your comments. >> >>>> >> >>>> >> >>>> >> >>>> Carol M. Torrence, HT(ASCP)CM >> >>>> >> >>>> >> >>>> >> >>>> >> >>>> >> >>>> _______________________________________________ >> >>>> Histonet mailing list >> >>>> Histonet@lists.utsouthwestern.edu >> >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>>> >> >>> _______________________________________________ >> >>> Histonet mailing list >> >>> Histonet@lists.utsouthwestern.edu >> >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> >> >> >> >> >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > ______________________________________________________________________ >> > This message is confidential, intended only for the named >> > recipient(s) and may contain information that is privileged >> > or exempt from disclosure under applicable law.? If you are >> > not the intended recipient(s), you are notified that the >> > dissemination, distribution, or copying of this message is >> > strictly prohibited.? If you receive this message in error, >> > or are not the named recipient(s), please notify the sender >> > at either the e-mail, fax, address, or telephone number >> > listed above and delete this e-mail from your computer. >> > Thank You. >> > ______________________________________________________________________ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Mon Apr 9 08:45:45 2012 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Mon Apr 9 08:45:55 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <1333977585.56151.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> References: <004601cd1360$ab3492f0$019db8d0$@com> <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com> <001901cd1435$83a5dd40$8af197c0$@com> <015703AD-C13F-4AF3-9C3C-2E733FCB4729@yumaregional.org> <1B804D2F-EE38-45BF-B4A5-87BD424061CA@yahoo.com> <4988.208.62.167.196.1333974550.squirrel@webmail.realpages.com> <1333977585.56151.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> Message-ID: <7EAFE982E328304DA6CE2B677BB762460BAD0374@TN001WEXMBX12.US.chs.net> The problem here seems to be that there are those who are upset that independent labs "seem" to take work away from hospitals. This in fact is probably true, but I for one have more than enough work for the 4 of us. All independent labs are inspected and have regulations to follow. We are all professionals. The days of hiring folks off the street to perform semi complex testing are over. Not everyone can work in a hospital setting. There is enough work out there for all of us regardless of where the work is performed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Monday, April 09, 2012 9:20 AM To: Nicole Tatum; Histonet Subject: Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs Bravo Nicole! ? Yet another pompous post from D. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Nicole Tatum To: Davide Costanzo ; histonet@lists.utsouthwestern.edu Sent: Monday, April 9, 2012 7:29 AM Subject: Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs Wow David, I strongly disagree with you. I happen to work and run at Dermatopathology lab and I am a licensed Histotechnologist. I have worked in the field for twelve years and I produce the same quality of work that I would if I was in a hospital. I also have to comply with the same state and CLIA rules you do. If not we woundlt be "treading on thim ice" we would be closed down. CLIA would not let my facility remain open if I was not producing the quality of work expected from all histology laboratories. Also, I cannt believe you would want our labs closed down. Do you know how many of YOUR FELLOW histologist you would put out of work. David is such a team player!!!! In reguards to stark laws. Maybe you should read what it actually means!!!! We only process specimens within our our facility and our slides ARE read by a licensed dermatopathologist. So, please tell me how my work is any less important than ur hospital job? And let me tell you this about my mediocker job. I work M-F 9-5. no weekends and no on-call. I also have full benefits and 401K. So, Im sorry that you feel our labs are some how underqualified, but I would not go back to hospital work, to save my life. Thanks for having my back, your fellow histologist. Nicole Tatum, HT ASCP This is a fascinating thread!! > > So what are your thoughts on this - It would appear that, if other > insurers follow suit, this could pose a huge burden on dermatologists > that do their own tissue processing, and all the GI labs across the > country that are popping up doing their own in-house histology. It may > be hard, and in some cases not possible for those labs to become CAP > accredited. > > In my opinion, that would be a great thing, to see all those physician > offices doing histology close their lab doors, and focus on thier own > specialty rather than invade the pathology world from which they were > not trained. It would seem, to the average witness, that these > facilities are treading on very thin ice as it is. It certainly does > appear to be a violation of Stark laws that were created for a very > good reason. In addition, these offices have stolen the "bread and > butter" from large labs, and hospital pathology departments and left > behind the far less profitable work. It would be nice to see that work > return to the place it belongs - in Pathology laboratories. > > It may be a pipe dream at this point, but who knows - maybe this is > the start of a very, very good thing. > > > > On Fri, Apr 6, 2012 at 2:56 PM, Kim Donadio > wrote: > >> Yikes I just 2 sec ago said that lol >> >> Sent from my iPhone >> >> On Apr 6, 2012, at 4:51 PM, Jesus Ellin wrote: >> >> > There are several frame of minds here, but most closely this aligns >> with >> the affordable care act and quality outcomes for patients.? I to >> agree with the statement that other agencies can provide good quality >> outcomes, but Anatomic pathology is changing so rapidly.? From all >> aspects, but if you look at who bills for most of the CMS testing it >> falls under hospital based laboratories, yet the government decides >> reimbursement based on what the large labs make..? In the end we are >> seeing consolidation,, but I hope someone comes to the forefront to >> speak for us all. >> > Sent from my iPad >> > On Apr 6, 2012, at 1:41 PM, "Carol Torrence" >> wrote: >> > >> >> I too have been through many CAP inspections in the past. Passing >> >> is >> not my >> >> concern - how about expense, prep time, time away to inspect a peer. >>? We >> >> are a small private lab also so this a bit of a pain.? There is no >> way >> that >> >> CAP will be able to accommodate the workload that will ensue if >> >> this >> becomes >> >> a trend. Which I think it will and there will be more insurance >> companies >> >> aligning themselves with the larger labs as "preferred".? My fear >> >> is >> that >> >> local healthcare will be so undercut that it will become more >> difficult >> if >> >> not impossible for even hospital labs to compete. I will never be >> convinced >> >> that big is better. >> >> >> >> I believe Aetna will hear from CAP on this issue due to the >> >> increased workload to them...deadlines may have to be extended.? >> >> We are hearing >> from a >> >> CAP member that they will not be able to be accredited in such a >> short >> time, >> >> according to CAP. >> >> >> >> -----Original Message----- >> >> From: Kim Donadio [mailto:one_angel_secret@yahoo.com] >> >> Sent: Thursday, April 05, 2012 6:31 PM >> >> To: Katelin Lester >> >> Cc: Carol Torrence; histonet@lists.utsouthwestern.edu >> >> Subject: Re: [Histonet] Aetna requiring CAP accreditation for >> non-hospital >> >> labs >> >> >> >> My lab can pass any inspection I have no fear Bring it on >> >> utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s >> >> >> >> Sent from my iPhone >> >> >> >> On Apr 5, 2012, at 7:00 PM, Katelin Lester >> >> >> wrote: >> >> >> >>> We also received this notice. We contacted our local CLIA office >> >>> who had heard of it this week as well. We are a small lab, so we >> >>> are not sure yet how this change will impact us. I'd also be >> >>> curious to know what smaller, private labs are planning on doing. >> >>> -- >> >>> Katelin Lester, HTL >> >>> Gastroenterology Specialists of Oregon, P.C. >> >>> Pathology Laboratory >> >>> (971) 224-2408 >> >>> >> >>> On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence >> >> >> wrote: >> >>> >> >>>> We have received notification from AETNA that they now require >> >>>> non-hospital labs to be accredited by CLIA and CAP.? The letter >> makes >> >>>> it obvious that by making such a request that they are not aware >> that >> >>>> CLIA assigned deemed status to CAP and CLIA is actually the >> >>>> gatekeeper.? Secondly we are told to be registered by May 1st >> >>>> and accredited by August 1st (which CAP says is >> >>>> impossible) or we will have to send our lab to either Quest or >> >>>> Ameripath which includes Dermpath Diagnostics division.? It >> >>>> fails >> to >> >>>> mention that there are other CAP accredited non hospital labs in >> our >> >>>> state.? The Aetna contact number is either 'mailbox full" or >> >>>> even after leaving a message, no return call.? Me thinks me >> >>>> smells a >> rat. >> >>>> >> >>>> >> >>>> >> >>>> If you are a non-hospital lab, have you heard of this?? Does >> >>>> your dematopathologist or pathologist know this is coming?? I am >> >>>> interested in your comments. >> >>>> >> >>>> >> >>>> >> >>>> Carol M. Torrence, HT(ASCP)CM >> >>>> >> >>>> >> >>>> >> >>>> >> >>>> >> >>>> _______________________________________________ >> >>>> Histonet mailing list >> >>>> Histonet@lists.utsouthwestern.edu >> >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>>> >> >>> _______________________________________________ >> >>> Histonet mailing list >> >>> Histonet@lists.utsouthwestern.edu >> >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> >> >> >> >> >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > ___________________________________________________________________ >> > ___ This message is confidential, intended only for the named >> > recipient(s) and may contain information that is privileged or >> > exempt from disclosure under applicable law.? If you are not the >> > intended recipient(s), you are notified that the dissemination, >> > distribution, or copying of this message is strictly prohibited.? >> > If you receive this message in error, or are not the named >> > recipient(s), please notify the sender at either the e-mail, fax, >> > address, or telephone number listed above and delete this e-mail >> > from your computer. >> > Thank You. >> > ___________________________________________________________________ >> > ___ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From lhadley <@t> iupui.edu Mon Apr 9 10:06:50 2012 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Mon Apr 9 10:06:57 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <1333977585.56151.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> References: <004601cd1360$ab3492f0$019db8d0$@com> <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com> <001901cd1435$83a5dd40$8af197c0$@com> <015703AD-C13F-4AF3-9C3C-2E733FCB4729@yumaregional.org> <1B804D2F-EE38-45BF-B4A5-87BD424061CA@yahoo.com> <4988.208.62.167.196.1333974550.squirrel@webmail.realpages.com> <1333977585.56151.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> Message-ID: <8638FBDA16B0584D82AA21CD236FF97F2DD8FB2E@IU-MSSG-MBX110.ads.iu.edu> Ditto Paula! Lee Ann Baldridge IUSM Indpls., IN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Monday, April 09, 2012 9:20 AM To: Nicole Tatum; Histonet Subject: Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs Bravo Nicole! ? Yet another pompous post from D. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Nicole Tatum To: Davide Costanzo ; histonet@lists.utsouthwestern.edu Sent: Monday, April 9, 2012 7:29 AM Subject: Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs Wow David, I strongly disagree with you. I happen to work and run at Dermatopathology lab and I am a licensed Histotechnologist. I have worked in the field for twelve years and I produce the same quality of work that I would if I was in a hospital. I also have to comply with the same state and CLIA rules you do. If not we woundlt be "treading on thim ice" we would be closed down. CLIA would not let my facility remain open if I was not producing the quality of work expected from all histology laboratories. Also, I cannt believe you would want our labs closed down. Do you know how many of YOUR FELLOW histologist you would put out of work. David is such a team player!!!! In reguards to stark laws. Maybe you should read what it actually means!!!! We only process specimens within our our facility and our slides ARE read by a licensed dermatopathologist. So, please tell me how my work is any less important than ur hospital job? And let me tell you this about my mediocker job. I work M-F 9-5. no weekends and no on-call. I also have full benefits and 401K. So, Im sorry that you feel our labs are some how underqualified, but I would not go back to hospital work, to save my life. Thanks for having my back, your fellow histologist. Nicole Tatum, HT ASCP This is a fascinating thread!! > > So what are your thoughts on this - It would appear that, if other > insurers > follow suit, this could pose a huge burden on dermatologists that do their > own tissue processing, and all the GI labs across the country that are > popping up doing their own in-house histology. It may be hard, and in some > cases not possible for those labs to become CAP accredited. > > In my opinion, that would be a great thing, to see all those physician > offices doing histology close their lab doors, and focus on thier own > specialty rather than invade the pathology world from which they were not > trained. It would seem, to the average witness, that these facilities are > treading on very thin ice as it is. It certainly does appear to be a > violation of Stark laws that were created for a very good reason. In > addition, these offices have stolen the "bread and butter" from large > labs, > and hospital pathology departments and left behind the far less profitable > work. It would be nice to see that work return to the place it belongs - > in > Pathology laboratories. > > It may be a pipe dream at this point, but who knows - maybe this is the > start of a very, very good thing. > > > > On Fri, Apr 6, 2012 at 2:56 PM, Kim Donadio > wrote: > >> Yikes I just 2 sec ago said that lol >> >> Sent from my iPhone >> >> On Apr 6, 2012, at 4:51 PM, Jesus Ellin wrote: >> >> > There are several frame of minds here, but most closely this aligns >> with >> the affordable care act and quality outcomes for patients.? I to agree >> with >> the statement that other agencies can provide good quality outcomes, but >> Anatomic pathology is changing so rapidly.? From all aspects, but if you >> look at who bills for most of the CMS testing it falls under hospital >> based >> laboratories, yet the government decides reimbursement based on what the >> large labs make..? In the end we are seeing consolidation,, but I hope >> someone comes to the forefront to speak for us all. >> > Sent from my iPad >> > On Apr 6, 2012, at 1:41 PM, "Carol Torrence" >> wrote: >> > >> >> I too have been through many CAP inspections in the past. Passing is >> not my >> >> concern - how about expense, prep time, time away to inspect a peer. >>? We >> >> are a small private lab also so this a bit of a pain.? There is no >> way >> that >> >> CAP will be able to accommodate the workload that will ensue if this >> becomes >> >> a trend. Which I think it will and there will be more insurance >> companies >> >> aligning themselves with the larger labs as "preferred".? My fear is >> that >> >> local healthcare will be so undercut that it will become more >> difficult >> if >> >> not impossible for even hospital labs to compete. I will never be >> convinced >> >> that big is better. >> >> >> >> I believe Aetna will hear from CAP on this issue due to the increased >> >> workload to them...deadlines may have to be extended.? We are hearing >> from a >> >> CAP member that they will not be able to be accredited in such a >> short >> time, >> >> according to CAP. >> >> >> >> -----Original Message----- >> >> From: Kim Donadio [mailto:one_angel_secret@yahoo.com] >> >> Sent: Thursday, April 05, 2012 6:31 PM >> >> To: Katelin Lester >> >> Cc: Carol Torrence; histonet@lists.utsouthwestern.edu >> >> Subject: Re: [Histonet] Aetna requiring CAP accreditation for >> non-hospital >> >> labs >> >> >> >> My lab can pass any inspection I have no fear Bring it on >> >> utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s >> >> >> >> Sent from my iPhone >> >> >> >> On Apr 5, 2012, at 7:00 PM, Katelin Lester >> wrote: >> >> >> >>> We also received this notice. We contacted our local CLIA office who >> >>> had heard of it this week as well. We are a small lab, so we are not >> >>> sure yet how this change will impact us. I'd also be curious to know >> >>> what smaller, private labs are planning on doing. >> >>> -- >> >>> Katelin Lester, HTL >> >>> Gastroenterology Specialists of Oregon, P.C. >> >>> Pathology Laboratory >> >>> (971) 224-2408 >> >>> >> >>> On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence >> >> >> wrote: >> >>> >> >>>> We have received notification from AETNA that they now require >> >>>> non-hospital labs to be accredited by CLIA and CAP.? The letter >> makes >> >>>> it obvious that by making such a request that they are not aware >> that >> >>>> CLIA assigned deemed status to CAP and CLIA is actually the >> >>>> gatekeeper.? Secondly we are told to be registered by May 1st and >> >>>> accredited by August 1st (which CAP says is >> >>>> impossible) or we will have to send our lab to either Quest or >> >>>> Ameripath which includes Dermpath Diagnostics division.? It fails >> to >> >>>> mention that there are other CAP accredited non hospital labs in >> our >> >>>> state.? The Aetna contact number is either 'mailbox full" or even >> >>>> after leaving a message, no return call.? Me thinks me smells a >> rat. >> >>>> >> >>>> >> >>>> >> >>>> If you are a non-hospital lab, have you heard of this?? Does your >> >>>> dematopathologist or pathologist know this is coming?? I am >> >>>> interested in your comments. >> >>>> >> >>>> >> >>>> >> >>>> Carol M. Torrence, HT(ASCP)CM >> >>>> >> >>>> >> >>>> >> >>>> >> >>>> >> >>>> _______________________________________________ >> >>>> Histonet mailing list >> >>>> Histonet@lists.utsouthwestern.edu >> >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>>> >> >>> _______________________________________________ >> >>> Histonet mailing list >> >>> Histonet@lists.utsouthwestern.edu >> >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> >> >> >> >> >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > ______________________________________________________________________ >> > This message is confidential, intended only for the named >> > recipient(s) and may contain information that is privileged >> > or exempt from disclosure under applicable law.? If you are >> > not the intended recipient(s), you are notified that the >> > dissemination, distribution, or copying of this message is >> > strictly prohibited.? If you receive this message in error, >> > or are not the named recipient(s), please notify the sender >> > at either the e-mail, fax, address, or telephone number >> > listed above and delete this e-mail from your computer. >> > Thank You. >> > ______________________________________________________________________ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Mon Apr 9 10:17:18 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Mon Apr 9 10:17:23 2012 Subject: [Histonet] Cassette labeling problem Message-ID: <4F82FD7E.5040202@mclean.harvard.edu> Hi All: Lately I have been having problems with the ink fading-and/or simply coming off in pieces-from our processing cassettes. I use Securline Marker 2/Superfrost pens. The writing seems to hold fine even if the cassettes sit in formalin for several weeks. So I am assuming that the problem comes either in the processing or embedding stage. I think the ink was still fine when I removed the cassette basket from the processor's retort, but I can't remember for sure. Then, I let the cassettes sit in Surgipath Embedding Media for two hours before embedding the tissue, since my Shandon XP processor has only 2 wax reservoirs. I have noticed lately that pieces of ink are coming off of the cassettes into the embedding media, making some-but not all-of the cassettes impossible to read.. I have turned down the temperature of the cassette holding tank, in case the temperature of the embedding media is pulling the ink off. Has anyone experienced this before, and if so, how did you overcome it? Are the Securline Marker 2/Superfrost pens appropriate for cassettes? Thank you for any advice you can provide, Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA. 617-855-3592 From algranth <@t> email.arizona.edu Mon Apr 9 10:17:57 2012 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Apr 9 10:18:11 2012 Subject: [Histonet] Lets talk forceps In-Reply-To: <0E4FA7061FCA498F90623F509F5554C0@prueggihctechlt> References: <5D59AF96B902456A8B5948EB08BACE79@PATHOLOGY> <0E4FA7061FCA498F90623F509F5554C0@prueggihctechlt> Message-ID: <2832E787-C516-488F-8B1B-D3E37B870C0F@email.arizona.edu> Here in Tucson we have the wonderful luxury of having the best (and largest) gem and minerals show in the country every February. I always head to the jewelry making tools table when I need forceps, scissors, spatulas, etc. Last time I bought some they were 3/$10. So if anybody is in need send me an email the begining of next February and I'll look for what you need. If you can't wait and don't have a Hobby Lobby near you try googling and I bet you will come up with some companies that sell fine forceps. Andi G On Apr 8, 2012, at 12:15 PM, Patsy Ruegg wrote: > I get my forceps, even the fine bent ones at Hobby Lobby in the jewelry > making section, they are much cheaper than the medical supply companies and > in my experience just as good. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Friday, April 06, 2012 1:03 PM > To: Bartlett, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Lets talk forceps > > Roboz makes great forceps and they aren't expensive. I think Storz may > have bought them out. > > Emily > > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that?s beautiful. > --Ron Swanson > > > > On Fri, Apr 6, 2012 at 9:43 AM, Bartlett, Jeanine (CDC/OID/NCEZID) < > jqb7@cdc.gov> wrote: > >> I really like these and I have small hands and have had carpal tunnel >> surgery. >> >> Surgipath ergonomic forceps now available through Leica: >> >> 38DI15585 >> 38DI15590 >> >> These both have a 5 ?" handle. One is curved and the other straight. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tiffeny Magee >> Sent: Friday, April 06, 2012 9:33 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Lets talk forceps >> >> I would love to buy a top of the line pair of forceps. One in partial that >> is smallish for a woman's hand and most importantly doesn't stick to the >> tissue on the water bath when I'm separating my sections. So does anyone >> have histology HT forceps they highly recommend? >> >> >> >> Thanks >> >> Tiffeny Magee >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lblazek <@t> digestivespecialists.com Mon Apr 9 10:19:44 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Apr 9 10:19:51 2012 Subject: [Histonet] Cassette labeling problem In-Reply-To: <4F82FD7E.5040202@mclean.harvard.edu> References: <4F82FD7E.5040202@mclean.harvard.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E3913819D43CA@IBMB7Exchange.digestivespecialists.com> It may be your cassette and not your marker. That was the problem we encountered awhile ago. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Monday, April 09, 2012 11:17 AM To: Histonet Subject: [Histonet] Cassette labeling problem Hi All: Lately I have been having problems with the ink fading-and/or simply coming off in pieces-from our processing cassettes. I use Securline Marker 2/Superfrost pens. The writing seems to hold fine even if the cassettes sit in formalin for several weeks. So I am assuming that the problem comes either in the processing or embedding stage. I think the ink was still fine when I removed the cassette basket from the processor's retort, but I can't remember for sure. Then, I let the cassettes sit in Surgipath Embedding Media for two hours before embedding the tissue, since my Shandon XP processor has only 2 wax reservoirs. I have noticed lately that pieces of ink are coming off of the cassettes into the embedding media, making some-but not all-of the cassettes impossible to read.. I have turned down the temperature of the cassette holding tank, in case the temperature of the embedding media is pulling the ink off. Has anyone experienced this before, and if so, how did you overcome it? Are the Securline Marker 2/Superfrost pens appropriate for cassettes? Thank you for any advice you can provide, Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA. 617-855-3592 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Mon Apr 9 10:40:46 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Apr 9 10:41:51 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <4988.208.62.167.196.1333974550.squirrel@webmail.realpages.com> References: <004601cd1360$ab3492f0$019db8d0$@com> <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com> <001901cd1435$83a5dd40$8af197c0$@com> <015703AD-C13F-4AF3-9C3C-2E733FCB4729@yumaregional.org> <1B804D2F-EE38-45BF-B4A5-87BD424061CA@yahoo.com> <4988.208.62.167.196.1333974550.squirrel@webmail.realpages.com> Message-ID: <1333986046.48732.YahooMailNeo@web112302.mail.gq1.yahoo.com> I agree with Nicole. ? Davide, personally I think you went over the line. Many Histotechs out here are just as qualified and thier work is just as important as anyone elses. As a person who has worked in both situations, I think this date line is a bit unfair and your comment uncalled for. ? I spoke with CAP this morning and they agree the time frame is too short. I am told they have contacted Aetna to try and get some kind of leeway for people who have at least applied as they tell me there is no way we can get accreditation by that deadline.They are bombed with calls/applications.? ? With all this said, and my ego now put back in my pocket. We need to support each other as professionals of our feild. These are hard times for healthcare professionals all around with many new regualtions around the bend. So lets try to stick together as a group of professionals and I suggest we all contact Aetna, and any governmental agency's we can regarding this. ? Because what starts here with one can certainly get out of hand rapidly; and dont always think you'll be on the side thats not getting the hit. Situations change. ? Best Regards ? Kim D ? ________________________________ From: Nicole Tatum To: Davide Costanzo ; histonet@lists.utsouthwestern.edu Sent: Monday, April 9, 2012 8:29 AM Subject: Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs Wow David, I strongly disagree with you. I happen to work and run at Dermatopathology lab and I am a licensed Histotechnologist. I have worked in the field for twelve years and I produce the same quality of work that I would if I was in a hospital. I also have to comply with the same state and CLIA rules you do. If not we woundlt be "treading on thim ice" we would be closed down. CLIA would not let my facility remain open if I was not producing the quality of work expected from all histology laboratories. Also, I cannt believe you would want our labs closed down. Do you know how many of YOUR FELLOW histologist you would put out of work. David is such a team player!!!! In reguards to stark laws. Maybe you should read what it actually means!!!! We only process specimens within our our facility and our slides ARE read by a licensed dermatopathologist. So, please tell me how my work is any less important than ur hospital job? And let me tell you this about my mediocker job. I work M-F 9-5. no weekends and no on-call. I also have full benefits and 401K. So, Im sorry that you feel our labs are some how underqualified, but I would not go back to hospital work, to save my life. Thanks for having my back, your fellow histologist. Nicole Tatum, HT ASCP This is a fascinating thread!! > > So what are your thoughts on this - It would appear that, if other > insurers > follow suit, this could pose a huge burden on dermatologists that do their > own tissue processing, and all the GI labs across the country that are > popping up doing their own in-house histology. It may be hard, and in some > cases not possible for those labs to become CAP accredited. > > In my opinion, that would be a great thing, to see all those physician > offices doing histology close their lab doors, and focus on thier own > specialty rather than invade the pathology world from which they were not > trained. It would seem, to the average witness, that these facilities are > treading on very thin ice as it is. It certainly does appear to be a > violation of Stark laws that were created for a very good reason. In > addition, these offices have stolen the "bread and butter" from large > labs, > and hospital pathology departments and left behind the far less profitable > work. It would be nice to see that work return to the place it belongs - > in > Pathology laboratories. > > It may be a pipe dream at this point, but who knows - maybe this is the > start of a very, very good thing. > > > > On Fri, Apr 6, 2012 at 2:56 PM, Kim Donadio > wrote: > >> Yikes I just 2 sec ago said that lol >> >> Sent from my iPhone >> >> On Apr 6, 2012, at 4:51 PM, Jesus Ellin wrote: >> >> > There are several frame of minds here, but most closely this aligns >> with >> the affordable care act and quality outcomes for patients.? I to agree >> with >> the statement that other agencies can provide good quality outcomes, but >> Anatomic pathology is changing so rapidly.? From all aspects, but if you >> look at who bills for most of the CMS testing it falls under hospital >> based >> laboratories, yet the government decides reimbursement based on what the >> large labs make..? In the end we are seeing consolidation,, but I hope >> someone comes to the forefront to speak for us all. >> > Sent from my iPad >> > On Apr 6, 2012, at 1:41 PM, "Carol Torrence" >> wrote: >> > >> >> I too have been through many CAP inspections in the past. Passing is >> not my >> >> concern - how about expense, prep time, time away to inspect a peer. >>? We >> >> are a small private lab also so this a bit of a pain.? There is no >> way >> that >> >> CAP will be able to accommodate the workload that will ensue if this >> becomes >> >> a trend. Which I think it will and there will be more insurance >> companies >> >> aligning themselves with the larger labs as "preferred".? My fear is >> that >> >> local healthcare will be so undercut that it will become more >> difficult >> if >> >> not impossible for even hospital labs to compete. I will never be >> convinced >> >> that big is better. >> >> >> >> I believe Aetna will hear from CAP on this issue due to the increased >> >> workload to them...deadlines may have to be extended.? We are hearing >> from a >> >> CAP member that they will not be able to be accredited in such a >> short >> time, >> >> according to CAP. >> >> >> >> -----Original Message----- >> >> From: Kim Donadio [mailto:one_angel_secret@yahoo.com] >> >> Sent: Thursday, April 05, 2012 6:31 PM >> >> To: Katelin Lester >> >> Cc: Carol Torrence; histonet@lists.utsouthwestern.edu >> >> Subject: Re: [Histonet] Aetna requiring CAP accreditation for >> non-hospital >> >> labs >> >> >> >> My lab can pass any inspection I have no fear Bring it on >> >> utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s >> >> >> >> Sent from my iPhone >> >> >> >> On Apr 5, 2012, at 7:00 PM, Katelin Lester >> wrote: >> >> >> >>> We also received this notice. We contacted our local CLIA office who >> >>> had heard of it this week as well. We are a small lab, so we are not >> >>> sure yet how this change will impact us. I'd also be curious to know >> >>> what smaller, private labs are planning on doing. >> >>> -- >> >>> Katelin Lester, HTL >> >>> Gastroenterology Specialists of Oregon, P.C. >> >>> Pathology Laboratory >> >>> (971) 224-2408 >> >>> >> >>> On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence >> >> >> wrote: >> >>> >> >>>> We have received notification from AETNA that they now require >> >>>> non-hospital labs to be accredited by CLIA and CAP.? The letter >> makes >> >>>> it obvious that by making such a request that they are not aware >> that >> >>>> CLIA assigned deemed status to CAP and CLIA is actually the >> >>>> gatekeeper.? Secondly we are told to be registered by May 1st and >> >>>> accredited by August 1st (which CAP says is >> >>>> impossible) or we will have to send our lab to either Quest or >> >>>> Ameripath which includes Dermpath Diagnostics division.? It fails >> to >> >>>> mention that there are other CAP accredited non hospital labs in >> our >> >>>> state.? The Aetna contact number is either 'mailbox full" or even >> >>>> after leaving a message, no return call.? Me thinks me smells a >> rat. >> >>>> >> >>>> >> >>>> >> >>>> If you are a non-hospital lab, have you heard of this?? Does your >> >>>> dematopathologist or pathologist know this is coming?? I am >> >>>> interested in your comments. >> >>>> >> >>>> >> >>>> >> >>>> Carol M. Torrence, HT(ASCP)CM >> >>>> >> >>>> >> >>>> >> >>>> >> >>>> >> >>>> _______________________________________________ >> >>>> Histonet mailing list >> >>>> Histonet@lists.utsouthwestern.edu >> >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>>> >> >>> _______________________________________________ >> >>> Histonet mailing list >> >>> Histonet@lists.utsouthwestern.edu >> >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> >> >> >> >> >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > ______________________________________________________________________ >> > This message is confidential, intended only for the named >> > recipient(s) and may contain information that is privileged >> > or exempt from disclosure under applicable law.? If you are >> > not the intended recipient(s), you are notified that the >> > dissemination, distribution, or copying of this message is >> > strictly prohibited.? If you receive this message in error, >> > or are not the named recipient(s), please notify the sender >> > at either the e-mail, fax, address, or telephone number >> > listed above and delete this e-mail from your computer. >> > Thank You. >> > ______________________________________________________________________ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NHeath <@t> Lifespan.org Mon Apr 9 11:08:04 2012 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Mon Apr 9 11:08:46 2012 Subject: [Histonet] Cassette labeling problem In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3913819D43CA@IBMB7Exchange.digestivespecialists.com> References: <4F82FD7E.5040202@mclean.harvard.edu> <5A2BD13465E061429D6455C8D6B40E3913819D43CA@IBMB7Exchange.digestivespecialists.com> Message-ID: <130E8991F210424096EFC6F42EA33B2408D754E2@LSCOEXCH1.lsmaster.lifespan.org> @Linda...How did you figure out it was your cassettes? Sometimes I have a problem with my cassette marker coming off my cassettes too no matter what brand of cassette marker I use. Please let me know your secret :) What are the best brand of cassettes to use? Nancy Heath, HT (ASCP) Neuropathology Technician Pathology Tech Specialist Dept. of Pathology., Div. of Neuropathology Rhode Island Hospital APC Blding, Flr 12, Rm 211 593 Eddy Street Providence, RI 02903 lab: 401-444-3246 fax: 401-444-8514 nheath@lifespan.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, April 09, 2012 11:20 AM To: 'Tim Wheelock'; Histonet Subject: RE: [Histonet] Cassette labeling problem It may be your cassette and not your marker. That was the problem we encountered awhile ago. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Monday, April 09, 2012 11:17 AM To: Histonet Subject: [Histonet] Cassette labeling problem Hi All: Lately I have been having problems with the ink fading-and/or simply coming off in pieces-from our processing cassettes. I use Securline Marker 2/Superfrost pens. The writing seems to hold fine even if the cassettes sit in formalin for several weeks. So I am assuming that the problem comes either in the processing or embedding stage. I think the ink was still fine when I removed the cassette basket from the processor's retort, but I can't remember for sure. Then, I let the cassettes sit in Surgipath Embedding Media for two hours before embedding the tissue, since my Shandon XP processor has only 2 wax reservoirs. I have noticed lately that pieces of ink are coming off of the cassettes into the embedding media, making some-but not all-of the cassettes impossible to read.. I have turned down the temperature of the cassette holding tank, in case the temperature of the embedding media is pulling the ink off. Has anyone experienced this before, and if so, how did you overcome it? Are the Securline Marker 2/Superfrost pens appropriate for cassettes? Thank you for any advice you can provide, Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA. 617-855-3592 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathlocums <@t> gmail.com Mon Apr 9 11:31:55 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Mon Apr 9 11:32:02 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: <1333986046.48732.YahooMailNeo@web112302.mail.gq1.yahoo.com> References: <004601cd1360$ab3492f0$019db8d0$@com> <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com> <001901cd1435$83a5dd40$8af197c0$@com> <015703AD-C13F-4AF3-9C3C-2E733FCB4729@yumaregional.org> <1B804D2F-EE38-45BF-B4A5-87BD424061CA@yahoo.com> <4988.208.62.167.196.1333974550.squirrel@webmail.realpages.com> <1333986046.48732.YahooMailNeo@web112302.mail.gq1.yahoo.com> Message-ID: Kim, I agree that there must be broad based support for all the histotech's working in those offices. Again, several read something into my post that was not in it. Nowhere did I mention the techs, nor express any concern over the quality of those techs. I am quite sure some of the best histotechs in America work in those settings. I would think that the majority of the techs working in those offices would find new jobs popping up all over if those labs were forced to close. The work still needs to get done, so I think assuming there would be hundreds of techs out of work is not realistic. Hopefully someone out there knows the answer to this question - I have heard (cannot confirm) that these types of labs in physician offices are banned in some states already. Pennsylvania was mentioned once at a conference as being one of those states. Does anyone out there know of this, and if it is true? I cannot find info in print, which I prefer to do before commenting. One Medical Director local to me at a major University stated that there is a push now to spread law through some Congressional hearings currently under way to force the closure of physician owned labs of that sort on a Federal level. I cannot confirm this either, however it does seem logical to think that the powers in Pathology would be fighting hard behind closed doors to figure out a way to shut these places down. There are problems in healthcare in this country on so many levels, this is just another example of one of many. The Aetna issue is not entirely related to this scenario, but in the end it still may have the desired effect by those that are pushing for office lab closure if it catches on with other insurance providers. I do think there is a lot going on behind the scenes here that none of us are aware of. Where did this Aetna letter come from? What sparked it? It shouldn't be any surprise to find out that physicians in larger pathology departments, or pathology organizations are behind this in some capacity. I share the opinion of my former medical director that these labs are short-lived, that the government will eventually shut them down. This is kick-back, no matter how you slice it. Loopholes that exist today, are likely to be removed tomorrow. The lucrative business they experience today is enough to keep them in the game, but I think they know the risks, and are aware of the fact that this party will end at some point. It is not pompous, and I resent that allegation. How would dermatologists feel if we decided to do punch biopsies in pathology labs? If a pathologist is not allowed to do colonoscpoy, why is a GI doc allowed to do histology? My comment is simple - they should not be allowed to. Practice of medicine should be limited to what you are trained in, not what makes you the most cash. Greed is a big part of what is destroying healthcare in this country. As for histotechs, I fully support the profession. To suggest otherwise is a tainted opinion, and not factual at all. I have worked alongside techs for 24 years, and clearly value every aspect of what they do. It is not an easy job, it is often thankless and that is unfortunate. This subject has nothing to do with the techs, and for those that love their job in these physician office labs I do feel sorry for, as that is not a job that will be around forever by all indications. On Mon, Apr 9, 2012 at 8:40 AM, Kim Donadio wrote: > I agree with Nicole. > > Davide, personally I think you went over the line. Many Histotechs out > here are just as qualified and thier work is just as important as anyone > elses. As a person who has worked in both situations, I think this date > line is a bit unfair and your comment uncalled for. > > I spoke with CAP this morning and they agree the time frame is too short. > I am told they have contacted Aetna to try and get some kind of leeway for > people who have at least applied as they tell me there is no way we can get > accreditation by that deadline.They are bombed with calls/applications. > > With all this said, and my ego now put back in my pocket. We need to > support each other as professionals of our feild. These are hard times for > healthcare professionals all around with many new regualtions around the > bend. So lets try to stick together as a group of professionals and I > suggest we all contact Aetna, and any governmental agency's we can > regarding this. > > Because what starts here with one can certainly get out of hand rapidly; > and dont always think you'll be on the side thats not getting the hit. > Situations change. > > Best Regards > > Kim D > > *From:* Nicole Tatum > *To:* Davide Costanzo ; > histonet@lists.utsouthwestern.edu > *Sent:* Monday, April 9, 2012 8:29 AM > > *Subject:* Re: [Histonet] Aetna requiring CAP accreditation for > non-hospital labs > > Wow David, > > I strongly disagree with you. I happen to work and run at Dermatopathology > lab and I am a licensed Histotechnologist. I have worked in the field for > twelve years and I produce the same quality of work that I would if I was > in a hospital. I also have to comply with the same state and CLIA rules > you do. If not we woundlt be "treading on thim ice" we would be closed > down. CLIA would not let my facility remain open if I was not producing > the quality of work expected from all histology laboratories. Also, I > cannt believe you would want our labs closed down. Do you know how many of > YOUR FELLOW histologist you would put out of work. David is such a team > player!!!! In reguards to stark laws. Maybe you should read what it > actually means!!!! We only process specimens within our our facility and > our slides ARE read by a licensed dermatopathologist. So, please tell me > how my work is any less important than ur hospital job? And let me tell > you this about my mediocker job. I work M-F 9-5. no weekends and no > on-call. I also have full benefits and 401K. So, Im sorry that you feel > our labs are some how underqualified, but I would not go back to hospital > work, to save my life. Thanks for having my back, your fellow histologist. > > Nicole Tatum, HT ASCP > > > > This is a fascinating thread!! > > > > So what are your thoughts on this - It would appear that, if other > > insurers > > follow suit, this could pose a huge burden on dermatologists that do > their > > own tissue processing, and all the GI labs across the country that are > > popping up doing their own in-house histology. It may be hard, and in > some > > cases not possible for those labs to become CAP accredited. > > > > In my opinion, that would be a great thing, to see all those physician > > offices doing histology close their lab doors, and focus on thier own > > specialty rather than invade the pathology world from which they were not > > trained. It would seem, to the average witness, that these facilities are > > treading on very thin ice as it is. It certainly does appear to be a > > violation of Stark laws that were created for a very good reason. In > > addition, these offices have stolen the "bread and butter" from large > > labs, > > and hospital pathology departments and left behind the far less > profitable > > work. It would be nice to see that work return to the place it belongs - > > in > > Pathology laboratories. > > > > It may be a pipe dream at this point, but who knows - maybe this is the > > start of a very, very good thing. > > > > > > > > On Fri, Apr 6, 2012 at 2:56 PM, Kim Donadio > > wrote: > > > >> Yikes I just 2 sec ago said that lol > >> > >> Sent from my iPhone > >> > >> On Apr 6, 2012, at 4:51 PM, Jesus Ellin > wrote: > >> > >> > There are several frame of minds here, but most closely this aligns > >> with > >> the affordable care act and quality outcomes for patients. I to agree > >> with > >> the statement that other agencies can provide good quality outcomes, but > >> Anatomic pathology is changing so rapidly. From all aspects, but if you > >> look at who bills for most of the CMS testing it falls under hospital > >> based > >> laboratories, yet the government decides reimbursement based on what the > >> large labs make.. In the end we are seeing consolidation,, but I hope > >> someone comes to the forefront to speak for us all. > >> > Sent from my iPad > >> > On Apr 6, 2012, at 1:41 PM, "Carol Torrence" > >> wrote: > >> > > >> >> I too have been through many CAP inspections in the past. Passing is > >> not my > >> >> concern - how about expense, prep time, time away to inspect a peer. > >> We > >> >> are a small private lab also so this a bit of a pain. There is no > >> way > >> that > >> >> CAP will be able to accommodate the workload that will ensue if this > >> becomes > >> >> a trend. Which I think it will and there will be more insurance > >> companies > >> >> aligning themselves with the larger labs as "preferred". My fear is > >> that > >> >> local healthcare will be so undercut that it will become more > >> difficult > >> if > >> >> not impossible for even hospital labs to compete. I will never be > >> convinced > >> >> that big is better. > >> >> > >> >> I believe Aetna will hear from CAP on this issue due to the increased > >> >> workload to them...deadlines may have to be extended. We are hearing > >> from a > >> >> CAP member that they will not be able to be accredited in such a > >> short > >> time, > >> >> according to CAP. > >> >> > >> >> -----Original Message----- > >> >> From: Kim Donadio [mailto:one_angel_secret@yahoo.com] > >> >> Sent: Thursday, April 05, 2012 6:31 PM > >> >> To: Katelin Lester > >> >> Cc: Carol Torrence; histonet@lists.utsouthwestern.edu > >> >> Subject: Re: [Histonet] Aetna requiring CAP accreditation for > >> non-hospital > >> >> labs > >> >> > >> >> My lab can pass any inspection I have no fear Bring it on > >> >> utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s > >> >> > >> >> Sent from my iPhone > >> >> > >> >> On Apr 5, 2012, at 7:00 PM, Katelin Lester > >> wrote: > >> >> > >> >>> We also received this notice. We contacted our local CLIA office who > >> >>> had heard of it this week as well. We are a small lab, so we are not > >> >>> sure yet how this change will impact us. I'd also be curious to know > >> >>> what smaller, private labs are planning on doing. > >> >>> -- > >> >>> Katelin Lester, HTL > >> >>> Gastroenterology Specialists of Oregon, P.C. > >> >>> Pathology Laboratory > >> >>> (971) 224-2408 > >> >>> > >> >>> On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence > >> > >> >> wrote: > >> >>> > >> >>>> We have received notification from AETNA that they now require > >> >>>> non-hospital labs to be accredited by CLIA and CAP. The letter > >> makes > >> >>>> it obvious that by making such a request that they are not aware > >> that > >> >>>> CLIA assigned deemed status to CAP and CLIA is actually the > >> >>>> gatekeeper. Secondly we are told to be registered by May 1st and > >> >>>> accredited by August 1st (which CAP says is > >> >>>> impossible) or we will have to send our lab to either Quest or > >> >>>> Ameripath which includes Dermpath Diagnostics division. It fails > >> to > >> >>>> mention that there are other CAP accredited non hospital labs in > >> our > >> >>>> state. The Aetna contact number is either 'mailbox full" or even > >> >>>> after leaving a message, no return call. Me thinks me smells a > >> rat. > >> >>>> > >> >>>> > >> >>>> > >> >>>> If you are a non-hospital lab, have you heard of this? Does your > >> >>>> dematopathologist or pathologist know this is coming? I am > >> >>>> interested in your comments. > >> >>>> > >> >>>> > >> >>>> > >> >>>> Carol M. Torrence, HT(ASCP)CM > >> >>>> > >> >>>> > >> >>>> > >> >>>> > >> >>>> > >> >>>> _______________________________________________ > >> >>>> Histonet mailing list > >> >>>> Histonet@lists.utsouthwestern.edu > >> >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> >>>> > >> >>> _______________________________________________ > >> >>> Histonet mailing list > >> >>> Histonet@lists.utsouthwestern.edu > >> >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> >> > >> >> > >> >> > >> >> > >> >> > >> >> _______________________________________________ > >> >> Histonet mailing list > >> >> Histonet@lists.utsouthwestern.edu > >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > >> > ______________________________________________________________________ > >> > This message is confidential, intended only for the named > >> > recipient(s) and may contain information that is privileged > >> > or exempt from disclosure under applicable law. If you are > >> > not the intended recipient(s), you are notified that the > >> > dissemination, distribution, or copying of this message is > >> > strictly prohibited. If you receive this message in error, > >> > or are not the named recipient(s), please notify the sender > >> > at either the e-mail, fax, address, or telephone number > >> > listed above and delete this e-mail from your computer. > >> > Thank You. > >> > ______________________________________________________________________ > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > > > > -- > > *David Costanzo, MHS, PA (ASCP)* > > Project Manager > > *Blufrog Path Lab Solutions* > > 9401 Wilshire Blvd. Ste 650 > > Beverly Hills, CA 90212 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From one_angel_secret <@t> yahoo.com Mon Apr 9 12:13:11 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Apr 9 12:13:31 2012 Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs In-Reply-To: References: <004601cd1360$ab3492f0$019db8d0$@com> <5C65B0FE-DC4B-4A7A-A5E3-337CEF7A7207@yahoo.com> <001901cd1435$83a5dd40$8af197c0$@com> <015703AD-C13F-4AF3-9C3C-2E733FCB4729@yumaregional.org> <1B804D2F-EE38-45BF-B4A5-87BD424061CA@yahoo.com> <4988.208.62.167.196.1333974550.squirrel@webmail.realpages.com> <1333986046.48732.YahooMailNeo@web112302.mail.gq1.yahoo.com> Message-ID: I can't really reply to all this. Maybe someone else can. I do not consider private owned labs as kick backs. They provide a service which does not need to be done in a hospital. They deserve to be paid. Seriously would you want all work to have to go to a hospital lab? I hope not. Would u want hospitals to have to do MOHs ? I hope not. Best wishes Kim D Sent from my iPhone On Apr 9, 2012, at 12:31 PM, Davide Costanzo wrote: > Kim, > > I agree that there must be broad based support for all the histotech's working in those offices. Again, several read something into my post that was not in it. Nowhere did I mention the techs, nor express any concern over the quality of those techs. I am quite sure some of the best histotechs in America work in those settings. > > I would think that the majority of the techs working in those offices would find new jobs popping up all over if those labs were forced to close. The work still needs to get done, so I think assuming there would be hundreds of techs out of work is not realistic. > > Hopefully someone out there knows the answer to this question - I have heard (cannot confirm) that these types of labs in physician offices are banned in some states already. Pennsylvania was mentioned once at a conference as being one of those states. Does anyone out there know of this, and if it is true? I cannot find info in print, which I prefer to do before commenting. One Medical Director local to me at a major University stated that there is a push now to spread law through some Congressional hearings currently under way to force the closure of physician owned labs of that sort on a Federal level. I cannot confirm this either, however it does seem logical to think that the powers in Pathology would be fighting hard behind closed doors to figure out a way to shut these places down. > > There are problems in healthcare in this country on so many levels, this is just another example of one of many. The Aetna issue is not entirely related to this scenario, but in the end it still may have the desired effect by those that are pushing for office lab closure if it catches on with other insurance providers. I do think there is a lot going on behind the scenes here that none of us are aware of. Where did this Aetna letter come from? What sparked it? It shouldn't be any surprise to find out that physicians in larger pathology departments, or pathology organizations are behind this in some capacity. > > I share the opinion of my former medical director that these labs are short-lived, that the government will eventually shut them down. This is kick-back, no matter how you slice it. Loopholes that exist today, are likely to be removed tomorrow. The lucrative business they experience today is enough to keep them in the game, but I think they know the risks, and are aware of the fact that this party will end at some point. > > It is not pompous, and I resent that allegation. How would dermatologists feel if we decided to do punch biopsies in pathology labs? If a pathologist is not allowed to do colonoscpoy, why is a GI doc allowed to do histology? My comment is simple - they should not be allowed to. Practice of medicine should be limited to what you are trained in, not what makes you the most cash. Greed is a big part of what is destroying healthcare in this country. > > As for histotechs, I fully support the profession. To suggest otherwise is a tainted opinion, and not factual at all. I have worked alongside techs for 24 years, and clearly value every aspect of what they do. It is not an easy job, it is often thankless and that is unfortunate. This subject has nothing to do with the techs, and for those that love their job in these physician office labs I do feel sorry for, as that is not a job that will be around forever by all indications. > > > On Mon, Apr 9, 2012 at 8:40 AM, Kim Donadio wrote: > I agree with Nicole. > > Davide, personally I think you went over the line. Many Histotechs out here are just as qualified and thier work is just as important as anyone elses. As a person who has worked in both situations, I think this date line is a bit unfair and your comment uncalled for. > > I spoke with CAP this morning and they agree the time frame is too short. I am told they have contacted Aetna to try and get some kind of leeway for people who have at least applied as they tell me there is no way we can get accreditation by that deadline.They are bombed with calls/applications. > > With all this said, and my ego now put back in my pocket. We need to support each other as professionals of our feild. These are hard times for healthcare professionals all around with many new regualtions around the bend. So lets try to stick together as a group of professionals and I suggest we all contact Aetna, and any governmental agency's we can regarding this. > > Because what starts here with one can certainly get out of hand rapidly; and dont always think you'll be on the side thats not getting the hit. Situations change. > > Best Regards > > Kim D > > From: Nicole Tatum > To: Davide Costanzo ; histonet@lists.utsouthwestern.edu > Sent: Monday, April 9, 2012 8:29 AM > > Subject: Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs > > Wow David, > > I strongly disagree with you. I happen to work and run at Dermatopathology > lab and I am a licensed Histotechnologist. I have worked in the field for > twelve years and I produce the same quality of work that I would if I was > in a hospital. I also have to comply with the same state and CLIA rules > you do. If not we woundlt be "treading on thim ice" we would be closed > down. CLIA would not let my facility remain open if I was not producing > the quality of work expected from all histology laboratories. Also, I > cannt believe you would want our labs closed down. Do you know how many of > YOUR FELLOW histologist you would put out of work. David is such a team > player!!!! In reguards to stark laws. Maybe you should read what it > actually means!!!! We only process specimens within our our facility and > our slides ARE read by a licensed dermatopathologist. So, please tell me > how my work is any less important than ur hospital job? And let me tell > you this about my mediocker job. I work M-F 9-5. no weekends and no > on-call. I also have full benefits and 401K. So, Im sorry that you feel > our labs are some how underqualified, but I would not go back to hospital > work, to save my life. Thanks for having my back, your fellow histologist. > > Nicole Tatum, HT ASCP > > > > This is a fascinating thread!! > > > > So what are your thoughts on this - It would appear that, if other > > insurers > > follow suit, this could pose a huge burden on dermatologists that do their > > own tissue processing, and all the GI labs across the country that are > > popping up doing their own in-house histology. It may be hard, and in some > > cases not possible for those labs to become CAP accredited. > > > > In my opinion, that would be a great thing, to see all those physician > > offices doing histology close their lab doors, and focus on thier own > > specialty rather than invade the pathology world from which they were not > > trained. It would seem, to the average witness, that these facilities are > > treading on very thin ice as it is. It certainly does appear to be a > > violation of Stark laws that were created for a very good reason. In > > addition, these offices have stolen the "bread and butter" from large > > labs, > > and hospital pathology departments and left behind the far less profitable > > work. It would be nice to see that work return to the place it belongs - > > in > > Pathology laboratories. > > > > It may be a pipe dream at this point, but who knows - maybe this is the > > start of a very, very good thing. > > > > > > > > On Fri, Apr 6, 2012 at 2:56 PM, Kim Donadio > > wrote: > > > >> Yikes I just 2 sec ago said that lol > >> > >> Sent from my iPhone > >> > >> On Apr 6, 2012, at 4:51 PM, Jesus Ellin wrote: > >> > >> > There are several frame of minds here, but most closely this aligns > >> with > >> the affordable care act and quality outcomes for patients. I to agree > >> with > >> the statement that other agencies can provide good quality outcomes, but > >> Anatomic pathology is changing so rapidly. From all aspects, but if you > >> look at who bills for most of the CMS testing it falls under hospital > >> based > >> laboratories, yet the government decides reimbursement based on what the > >> large labs make.. In the end we are seeing consolidation,, but I hope > >> someone comes to the forefront to speak for us all. > >> > Sent from my iPad > >> > On Apr 6, 2012, at 1:41 PM, "Carol Torrence" > >> wrote: > >> > > >> >> I too have been through many CAP inspections in the past. Passing is > >> not my > >> >> concern - how about expense, prep time, time away to inspect a peer. > >> We > >> >> are a small private lab also so this a bit of a pain. There is no > >> way > >> that > >> >> CAP will be able to accommodate the workload that will ensue if this > >> becomes > >> >> a trend. Which I think it will and there will be more insurance > >> companies > >> >> aligning themselves with the larger labs as "preferred". My fear is > >> that > >> >> local healthcare will be so undercut that it will become more > >> difficult > >> if > >> >> not impossible for even hospital labs to compete. I will never be > >> convinced > >> >> that big is better. > >> >> > >> >> I believe Aetna will hear from CAP on this issue due to the increased > >> >> workload to them...deadlines may have to be extended. We are hearing > >> from a > >> >> CAP member that they will not be able to be accredited in such a > >> short > >> time, > >> >> according to CAP. > >> >> > >> >> -----Original Message----- > >> >> From: Kim Donadio [mailto:one_angel_secret@yahoo.com] > >> >> Sent: Thursday, April 05, 2012 6:31 PM > >> >> To: Katelin Lester > >> >> Cc: Carol Torrence; histonet@lists.utsouthwestern.edu > >> >> Subject: Re: [Histonet] Aetna requiring CAP accreditation for > >> non-hospital > >> >> labs > >> >> > >> >> My lab can pass any inspection I have no fear Bring it on > >> >> utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s > >> >> > >> >> Sent from my iPhone > >> >> > >> >> On Apr 5, 2012, at 7:00 PM, Katelin Lester > >> wrote: > >> >> > >> >>> We also received this notice. We contacted our local CLIA office who > >> >>> had heard of it this week as well. We are a small lab, so we are not > >> >>> sure yet how this change will impact us. I'd also be curious to know > >> >>> what smaller, private labs are planning on doing. > >> >>> -- > >> >>> Katelin Lester, HTL > >> >>> Gastroenterology Specialists of Oregon, P.C. > >> >>> Pathology Laboratory > >> >>> (971) 224-2408 > >> >>> > >> >>> On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence > >> > >> >> wrote: > >> >>> > >> >>>> We have received notification from AETNA that they now require > >> >>>> non-hospital labs to be accredited by CLIA and CAP. The letter > >> makes > >> >>>> it obvious that by making such a request that they are not aware > >> that > >> >>>> CLIA assigned deemed status to CAP and CLIA is actually the > >> >>>> gatekeeper. Secondly we are told to be registered by May 1st and > >> >>>> accredited by August 1st (which CAP says is > >> >>>> impossible) or we will have to send our lab to either Quest or > >> >>>> Ameripath which includes Dermpath Diagnostics division. It fails > >> to > >> >>>> mention that there are other CAP accredited non hospital labs in > >> our > >> >>>> state. The Aetna contact number is either 'mailbox full" or even > >> >>>> after leaving a message, no return call. Me thinks me smells a > >> rat. > >> >>>> > >> >>>> > >> >>>> > >> >>>> If you are a non-hospital lab, have you heard of this? Does your > >> >>>> dematopathologist or pathologist know this is coming? I am > >> >>>> interested in your comments. > >> >>>> > >> >>>> > >> >>>> > >> >>>> Carol M. Torrence, HT(ASCP)CM > >> >>>> > >> >>>> > >> >>>> > >> >>>> > >> >>>> > >> >>>> _______________________________________________ > >> >>>> Histonet mailing list > >> >>>> Histonet@lists.utsouthwestern.edu > >> >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> >>>> > >> >>> _______________________________________________ > >> >>> Histonet mailing list > >> >>> Histonet@lists.utsouthwestern.edu > >> >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> >> > >> >> > >> >> > >> >> > >> >> > >> >> _______________________________________________ > >> >> Histonet mailing list > >> >> Histonet@lists.utsouthwestern.edu > >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > >> > ______________________________________________________________________ > >> > This message is confidential, intended only for the named > >> > recipient(s) and may contain information that is privileged > >> > or exempt from disclosure under applicable law. If you are > >> > not the intended recipient(s), you are notified that the > >> > dissemination, distribution, or copying of this message is > >> > strictly prohibited. If you receive this message in error, > >> > or are not the named recipient(s), please notify the sender > >> > at either the e-mail, fax, address, or telephone number > >> > listed above and delete this e-mail from your computer. > >> > Thank You. > >> > ______________________________________________________________________ > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > > > > -- > > *David Costanzo, MHS, PA (ASCP)* > > Project Manager > > *Blufrog Path Lab Solutions* > > 9401 Wilshire Blvd. Ste 650 > > Beverly Hills, CA 90212 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > David Costanzo, MHS, PA (ASCP) > Project Manager > Blufrog Path Lab Solutions > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > From jdcochran <@t> aol.com Mon Apr 9 12:52:47 2012 From: jdcochran <@t> aol.com (jdcochran@aol.com) Date: Mon Apr 9 12:53:06 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation Message-ID: <8CEE490B54C04D0-10D4-119D@webmail-m164.sysops.aol.com> Histonetters: I was informed today by The Joint Commission that an oversight was made in the original letter from Aetna regarding a new requirement for in-office AP lab accreditation. Aetna's Medical Director states in a letter to The Joint Commission: "It can be CAP or JCAHO certification of their laboratory. We want to encourage providers to obtain either one of these accreditations. We will be updating the physician letter with this change..." To my knowledge, TJC and CAP are the only CMS-deemed authorities for Anatomic Pathology lab accreditation since a third accreditation organization has exited that arena. In-office AP labs provide a valuable service to the practices they serve by facilitating 1) better communication between pathologists and ordering clinicians, 2) quality metrics that are practice-specific, and 3) high volume, sub-specialization for both histotechnologists and pathologists. In other words, the more of one type of histopathology a lab does (e.g., skin, prostate, GI), the better it gets. Most people would not think of having their cardiac bypass surgery done at a community hospital doing 50/year; you want to go where more than 500/year are done. In histopathology, the kinds of volume you want are in the thousands for each tissue type. Many hospital labs do little skin or prostate histology anymore. Many sub-specialty in-office AP labs may do thousands of cases of one tissue type every year. Aside from that, in-office AP labs are an emerging frontier of employment for histologists and pathologists. In an era of high unemployment, another source of employment for our professions is "a good thing." This requirement by an insurer for accreditation will help to validate these in-office AP labs' commitment to quality and put them on the level with their hospital counterparts. John D. Cochran, MD, FCAP From Gary_Steinke <@t> vwr.com Mon Apr 9 13:02:56 2012 From: Gary_Steinke <@t> vwr.com (Gary_Steinke@vwr.com) Date: Mon Apr 9 13:03:02 2012 Subject: [Histonet] Gary Steinke is out of the office Message-ID: I will be out of the office starting 04/06/2012 and will not return until 04/11/2012. I will be unavailable until at least April 11th due to a family emergency. If you need immediate help, please contact our Healthcare Customer Care Group at 877-881-1192 or by email at HEALTHCARESERVICE@VWR.COM. Thank you. From Kristopher.Kalleberg <@t> unilever.com Mon Apr 9 13:25:18 2012 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Mon Apr 9 13:25:25 2012 Subject: [Histonet] elastic stain Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C0DCD3FCA@NTRSEVS30002.s3.ms.unilever.com> All, I have used a new Verhoeff-Van Gieson stain kit (from BBC) for elastic fibers and have run a large number of slides. I am noticing differences in the staining intensities throughout the samples. I see that there are darker sites and lighter sites within the same panelists. This is not due to a treatment effect because these intensity differences are seen in the controls also. I am fairly confident that our tech has sectioned all the samples at 5um and has stained the slides with all the same timings. Does anyone have an explanation as to why this is occurring? The staining intensity differences are mainly in the epidermis but if that is staining darker/lighter my concern is that the elastic fibers in the dermis are doing the same and will skew the results when they are quantified with a spectral camera. Any help will be greatly appreciated. Thank you in advance. Kristopher L. Kalleberg Research Scientist Unilever R&D 40 Merritt Blvd. Trumbull, CT 06611 p 203.381.5765 f 203.381.5476 From Caroline.Pratt <@t> uphs.upenn.edu Mon Apr 9 14:09:21 2012 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Mon Apr 9 14:09:07 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: <8CEE490B54C04D0-10D4-119D@webmail-m164.sysops.aol.com> References: <8CEE490B54C04D0-10D4-119D@webmail-m164.sysops.aol.com> Message-ID: THANK YOU! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jdcochran@aol.com Sent: Monday, April 09, 2012 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Aetna and In-Office Lab Accreditation Histonetters: I was informed today by The Joint Commission that an oversight was made in the original letter from Aetna regarding a new requirement for in-office AP lab accreditation. Aetna's Medical Director states in a letter to The Joint Commission: "It can be CAP or JCAHO certification of their laboratory. We want to encourage providers to obtain either one of these accreditations. We will be updating the physician letter with this change..." To my knowledge, TJC and CAP are the only CMS-deemed authorities for Anatomic Pathology lab accreditation since a third accreditation organization has exited that arena. In-office AP labs provide a valuable service to the practices they serve by facilitating 1) better communication between pathologists and ordering clinicians, 2) quality metrics that are practice-specific, and 3) high volume, sub-specialization for both histotechnologists and pathologists. In other words, the more of one type of histopathology a lab does (e.g., skin, prostate, GI), the better it gets. Most people would not think of having their cardiac bypass surgery done at a community hospital doing 50/year; you want to go where more than 500/year are done. In histopathology, the kinds of volume you want are in the thousands for each tissue type. Many hospital labs do little skin or prostate histology anymore. Many sub-specialty in-office AP labs may do thousands of cases of one tissue type every year. Aside from that, in-office AP labs are an emerging frontier of employment for histologists and pathologists. In an era of high unemployment, another source of employment for our professions is "a good thing." This requirement by an insurer for accreditation will help to validate these in-office AP labs' commitment to quality and put them on the level with their hospital counterparts. John D. Cochran, MD, FCAP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From dlschneider <@t> gmail.com Mon Apr 9 15:47:04 2012 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Mon Apr 9 15:47:08 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: <8CEE490B54C04D0-10D4-119D@webmail-m164.sysops.aol.com> References: <8CEE490B54C04D0-10D4-119D@webmail-m164.sysops.aol.com> Message-ID: This is all about the money. The rest is rationalization. The reason a group of non-pathologist physicians opens an in-house pathology lab and hires an employee pathologist is first and foremost to harvest profit from pathology reimbursement. Be a fly on the wall in the partners' meetings and you would know that's what they are talking about. To suggest otherwise is disingenuous. And the implication that the generalist anatomic pathologist is unqualified to be signing out skins, prostates, GI's and whatever is reprehensible. This is not cardiac bypass surgery, and AP pathologists *are* trained to do all of the above. I eagerly defer to subspecialty expert consultants as needed, but most of the time they're not needed. Hospital labs that see few, if any skins, prostates, GI's, are only in that pickle because of the cherrypicking they've already been subjected to. *"in-office AP labs are an emerging frontier of employment for histologists and pathologists. In an era of high unemployment, another source of employment for our professions is "a good thing.""* Really? The jobs follow the specimens. Given the same number of specimens, there's the same number of jobs, more or less, just under different circumstances and in different locations Unless you're suggesting that in-office labs will generate increased specimens, and thus increased jobs though overutilization, i.e. excessive numbers of unnecessary biopsies and abuse of the patient and the taxpayer. In which case I have to say there's a grain of truth. And the truth hurts. And it's not " a good thing." None of this should be taken as criticism of histotechs and pathologists who find themselves working in an in-office lab. I know there's bills to pay, families to take care of, and god knows it's hard for a pathologist to find a job these days with the numbers our residency programs keep churning out (but that's another rant...). Dan Schneider, MD Amarillo, TX On Mon, Apr 9, 2012 at 12:52 PM, wrote: > > Histonetters: > > In-office AP labs provide a valuable service to the practices they serve > by facilitating 1) better communication between pathologists and ordering > clinicians, 2) quality metrics that are practice-specific, and 3) high > volume, sub-specialization for both histotechnologists and pathologists. > In other words, the more of one type of histopathology a lab does (e.g., > skin, prostate, GI), the better it gets. Most people would not think of > having their cardiac bypass surgery done at a community hospital doing > 50/year; you want to go where more than 500/year are done. In > histopathology, the kinds of volume you want are in the thousands for each > tissue type. Many hospital labs do little skin or prostate histology > anymore. Many sub-specialty in-office AP labs may do thousands of cases of > one tissue type every year. > > Aside from that, in-office AP labs are an emerging frontier of employment > for histologists and pathologists. In an era of high unemployment, another > source of employment for our professions is "a good thing." > > This requirement by an insurer for accreditation will help to validate > these in-office AP labs' commitment to quality and put them on the level > with their hospital counterparts. > > John D. Cochran, MD, FCAP > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pathlocums <@t> gmail.com Mon Apr 9 16:13:10 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Mon Apr 9 16:13:16 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation Message-ID: <3010968516548803193@unknownmsgid> Amen! Thank you Dr. Schneider. Sent from my Windows Phone From: Daniel Schneider Sent: 4/9/2012 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation This is all about the money. The rest is rationalization. The reason a group of non-pathologist physicians opens an in-house pathology lab and hires an employee pathologist is first and foremost to harvest profit from pathology reimbursement. Be a fly on the wall in the partners' meetings and you would know that's what they are talking about. To suggest otherwise is disingenuous. And the implication that the generalist anatomic pathologist is unqualified to be signing out skins, prostates, GI's and whatever is reprehensible. This is not cardiac bypass surgery, and AP pathologists *are* trained to do all of the above. I eagerly defer to subspecialty expert consultants as needed, but most of the time they're not needed. Hospital labs that see few, if any skins, prostates, GI's, are only in that pickle because of the cherrypicking they've already been subjected to. *"in-office AP labs are an emerging frontier of employment for histologists and pathologists. In an era of high unemployment, another source of employment for our professions is "a good thing.""* Really? The jobs follow the specimens. Given the same number of specimens, there's the same number of jobs, more or less, just under different circumstances and in different locations Unless you're suggesting that in-office labs will generate increased specimens, and thus increased jobs though overutilization, i.e. excessive numbers of unnecessary biopsies and abuse of the patient and the taxpayer. In which case I have to say there's a grain of truth. And the truth hurts. And it's not " a good thing." None of this should be taken as criticism of histotechs and pathologists who find themselves working in an in-office lab. I know there's bills to pay, families to take care of, and god knows it's hard for a pathologist to find a job these days with the numbers our residency programs keep churning out (but that's another rant...). Dan Schneider, MD Amarillo, TX On Mon, Apr 9, 2012 at 12:52 PM, wrote: > > Histonetters: > > In-office AP labs provide a valuable service to the practices they serve > by facilitating 1) better communication between pathologists and ordering > clinicians, 2) quality metrics that are practice-specific, and 3) high > volume, sub-specialization for both histotechnologists and pathologists. > In other words, the more of one type of histopathology a lab does (e.g., > skin, prostate, GI), the better it gets. Most people would not think of > having their cardiac bypass surgery done at a community hospital doing > 50/year; you want to go where more than 500/year are done. In > histopathology, the kinds of volume you want are in the thousands for each > tissue type. Many hospital labs do little skin or prostate histology > anymore. Many sub-specialty in-office AP labs may do thousands of cases of > one tissue type every year. > > Aside from that, in-office AP labs are an emerging frontier of employment > for histologists and pathologists. In an era of high unemployment, another > source of employment for our professions is "a good thing." > > This requirement by an insurer for accreditation will help to validate > these in-office AP labs' commitment to quality and put them on the level > with their hospital counterparts. > > John D. Cochran, MD, FCAP > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Mon Apr 9 16:57:42 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Apr 9 16:57:57 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: References: <8CEE490B54C04D0-10D4-119D@webmail-m164.sysops.aol.com> Message-ID: <19B9AB11-C858-4ED0-99CE-EF4939B448A5@yahoo.com> To suggest that any physician who goes into private practice and has their own lab is any more of a money hound than any other physician at a hospital would also be disingenuous . And of course this is about money. It's about one group of people trying to get another group of people out of the lab business because they want that money. It's also about the government squeezing insurance companies into these more stringent regulations. Now I'm not against more stringent regulations but I do find it offensive of how they are going about it. The little guy will take the hits on this one. I guess what they want is a bunch of walmart like labs. Private practices serve a patient care cause just as hospital labs do. They all make a diagnosis. They all deserve to be paid. My 2 cents Sent from my iPhone On Apr 9, 2012, at 4:47 PM, Daniel Schneider wrote: > This is all about the money. The rest is rationalization. > > The reason a group of non-pathologist physicians opens an in-house > pathology lab and hires an employee pathologist is first and foremost > to harvest profit from pathology reimbursement. Be a fly on the wall in the > partners' meetings and you would know that's what they are talking about. > > To suggest otherwise is disingenuous. > > And the implication that the generalist anatomic pathologist is unqualified > to be signing out skins, prostates, GI's and whatever is reprehensible. > This is not cardiac bypass surgery, and AP pathologists *are* trained to do > all of the above. I eagerly defer to subspecialty expert consultants as > needed, but most of the time they're not needed. > > Hospital labs that see few, if any skins, prostates, GI's, are only in that > pickle because of the cherrypicking they've already been subjected to. > > *"in-office AP labs are an emerging frontier of employment for histologists > and pathologists. In an era of high unemployment, another source of > employment for our professions is "a good thing.""* > > Really? The jobs follow the specimens. Given the same number of specimens, > there's the same number of jobs, more or less, just under different > circumstances and in different locations Unless you're suggesting that > in-office labs will generate increased specimens, and thus increased jobs > though overutilization, i.e. excessive numbers of unnecessary biopsies and > abuse of the patient and the taxpayer. In which case I have to say there's > a grain of truth. And the truth hurts. And it's not " a good thing." > > None of this should be taken as criticism of histotechs and pathologists > who find themselves working in an in-office lab. I know there's bills to > pay, families to take care of, and god knows it's hard for a pathologist to > find a job these days with the numbers our residency programs keep churning > out (but that's another rant...). > > Dan Schneider, MD > Amarillo, TX > > > > > > > > > > > > > On Mon, Apr 9, 2012 at 12:52 PM, wrote: > >> >> Histonetters: >> >> In-office AP labs provide a valuable service to the practices they serve >> by facilitating 1) better communication between pathologists and ordering >> clinicians, 2) quality metrics that are practice-specific, and 3) high >> volume, sub-specialization for both histotechnologists and pathologists. >> In other words, the more of one type of histopathology a lab does (e.g., >> skin, prostate, GI), the better it gets. Most people would not think of >> having their cardiac bypass surgery done at a community hospital doing >> 50/year; you want to go where more than 500/year are done. In >> histopathology, the kinds of volume you want are in the thousands for each >> tissue type. Many hospital labs do little skin or prostate histology >> anymore. Many sub-specialty in-office AP labs may do thousands of cases of >> one tissue type every year. >> >> Aside from that, in-office AP labs are an emerging frontier of employment >> for histologists and pathologists. In an era of high unemployment, another >> source of employment for our professions is "a good thing." >> >> This requirement by an insurer for accreditation will help to validate >> these in-office AP labs' commitment to quality and put them on the level >> with their hospital counterparts. >> >> John D. Cochran, MD, FCAP >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From larry <@t> foxlablogistics.com Mon Apr 9 17:28:06 2012 From: larry <@t> foxlablogistics.com (=?utf-8?B?bGFycnlAZm94bGFibG9naXN0aWNzLmNvbQ==?=) Date: Mon Apr 9 17:27:55 2012 Subject: [Histonet] (no subject) Message-ID: <20120409222753.22D4B2F24E980@gateway12.websitewelcome.com> Philosompephe,r From Rcartun <@t> harthosp.org Mon Apr 9 18:43:06 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Apr 9 18:43:18 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: References: <8CEE490B54C04D0-10D4-119D@webmail-m164.sysops.aol.com> Message-ID: <4F833BCA.7400.0077.1@harthosp.org> This was released today. Richard Statline Special Alert: New Evidence Links Self-Referral Labs to Increased Utilization, Lower Cancer Detection Rates Study co-funded by CAP Appears in April 2012 Issue of Health Affairs April 9?Self-referring urologists billed Medicare for nearly 75% more anatomic pathology (AP) specimens compared to non self-referring physicians, according to a study published today in a leading health care policy journal. Furthermore, the study found no increase in cancer detection for the patients of self-referring physicians-in fact, the detection rate was 14% lower than that of non self-referring physicians. These findings, from an independent study co-funded by the CAP, provide the first clear evidence that self-referral of anatomic pathology services leads to increased utilization, higher Medicare spending, and lower rates of cancer detection. The study, led by renowned Georgetown University health care economist Jean Mitchell, PhD, will appear in the April 2012 issue of Health Affairs and is now available on the journal?s website. -------------------------------------------------------------------------------- >>> Daniel Schneider 4/9/2012 4:47 PM >>> This is all about the money. The rest is rationalization. The reason a group of non-pathologist physicians opens an in-house pathology lab and hires an employee pathologist is first and foremost to harvest profit from pathology reimbursement. Be a fly on the wall in the partners' meetings and you would know that's what they are talking about. To suggest otherwise is disingenuous. And the implication that the generalist anatomic pathologist is unqualified to be signing out skins, prostates, GI's and whatever is reprehensible. This is not cardiac bypass surgery, and AP pathologists *are* trained to do all of the above. I eagerly defer to subspecialty expert consultants as needed, but most of the time they're not needed. Hospital labs that see few, if any skins, prostates, GI's, are only in that pickle because of the cherrypicking they've already been subjected to. *"in-office AP labs are an emerging frontier of employment for histologists and pathologists. In an era of high unemployment, another source of employment for our professions is "a good thing.""* Really? The jobs follow the specimens. Given the same number of specimens, there's the same number of jobs, more or less, just under different circumstances and in different locations Unless you're suggesting that in-office labs will generate increased specimens, and thus increased jobs though overutilization, i.e. excessive numbers of unnecessary biopsies and abuse of the patient and the taxpayer. In which case I have to say there's a grain of truth. And the truth hurts. And it's not " a good thing." None of this should be taken as criticism of histotechs and pathologists who find themselves working in an in-office lab. I know there's bills to pay, families to take care of, and god knows it's hard for a pathologist to find a job these days with the numbers our residency programs keep churning out (but that's another rant...). Dan Schneider, MD Amarillo, TX On Mon, Apr 9, 2012 at 12:52 PM, wrote: > > Histonetters: > > In-office AP labs provide a valuable service to the practices they serve > by facilitating 1) better communication between pathologists and ordering > clinicians, 2) quality metrics that are practice-specific, and 3) high > volume, sub-specialization for both histotechnologists and pathologists. > In other words, the more of one type of histopathology a lab does (e.g., > skin, prostate, GI), the better it gets. Most people would not think of > having their cardiac bypass surgery done at a community hospital doing > 50/year; you want to go where more than 500/year are done. In > histopathology, the kinds of volume you want are in the thousands for each > tissue type. Many hospital labs do little skin or prostate histology > anymore. Many sub-specialty in-office AP labs may do thousands of cases of > one tissue type every year. > > Aside from that, in-office AP labs are an emerging frontier of employment > for histologists and pathologists. In an era of high unemployment, another > source of employment for our professions is "a good thing." > > This requirement by an insurer for accreditation will help to validate > these in-office AP labs' commitment to quality and put them on the level > with their hospital counterparts. > > John D. Cochran, MD, FCAP > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathlocums <@t> gmail.com Mon Apr 9 19:00:44 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Mon Apr 9 19:00:48 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: <4F833BCA.7400.0077.1@harthosp.org> References: <8CEE490B54C04D0-10D4-119D@webmail-m164.sysops.aol.com> <4F833BCA.7400.0077.1@harthosp.org> Message-ID: Thank you for that. How are things at Hartford Hospital? One of my favorite places, rotated there many years ago. Very impressive facility! Is Dr. Ricci still there? On Mon, Apr 9, 2012 at 4:43 PM, Richard Cartun wrote: > This was released today. > > Richard > > Statline Special Alert: > New Evidence Links Self-Referral Labs to Increased Utilization, Lower > Cancer Detection Rates > Study co-funded by CAP Appears in April 2012 Issue of Health Affairs > April 9?Self-referring urologists billed Medicare for nearly 75% more > anatomic pathology (AP) specimens compared to non self-referring > physicians, according to a study published today in a leading health > care policy journal. Furthermore, the study found no increase in cancer > detection for the patients of self-referring physicians-in fact, the > detection rate was 14% lower than that of non self-referring > physicians. > > These findings, from an independent study co-funded by the CAP, provide > the first clear evidence that self-referral of anatomic pathology > services leads to increased utilization, higher Medicare spending, and > lower rates of cancer detection. The study, led by renowned Georgetown > University health care economist Jean Mitchell, PhD, will appear in the > April 2012 issue of Health Affairs and is now available on the > journal?s website. > > > -------------------------------------------------------------------------------- > > > >>> Daniel Schneider 4/9/2012 4:47 PM >>> > This is all about the money. The rest is rationalization. > > The reason a group of non-pathologist physicians opens an in-house > pathology lab and hires an employee pathologist is first and foremost > to harvest profit from pathology reimbursement. Be a fly on the wall in > the > partners' meetings and you would know that's what they are talking > about. > > To suggest otherwise is disingenuous. > > And the implication that the generalist anatomic pathologist is > unqualified > to be signing out skins, prostates, GI's and whatever is > reprehensible. > This is not cardiac bypass surgery, and AP pathologists *are* trained > to do > all of the above. I eagerly defer to subspecialty expert consultants > as > needed, but most of the time they're not needed. > > Hospital labs that see few, if any skins, prostates, GI's, are only in > that > pickle because of the cherrypicking they've already been subjected to. > > *"in-office AP labs are an emerging frontier of employment for > histologists > and pathologists. In an era of high unemployment, another source of > employment for our professions is "a good thing.""* > > Really? The jobs follow the specimens. Given the same number of > specimens, > there's the same number of jobs, more or less, just under different > circumstances and in different locations Unless you're suggesting > that > in-office labs will generate increased specimens, and thus increased > jobs > though overutilization, i.e. excessive numbers of unnecessary biopsies > and > abuse of the patient and the taxpayer. In which case I have to say > there's > a grain of truth. And the truth hurts. And it's not " a good thing." > > None of this should be taken as criticism of histotechs and > pathologists > who find themselves working in an in-office lab. I know there's bills > to > pay, families to take care of, and god knows it's hard for a > pathologist to > find a job these days with the numbers our residency programs keep > churning > out (but that's another rant...). > > Dan Schneider, MD > Amarillo, TX > > > > > > > > > > > > > On Mon, Apr 9, 2012 at 12:52 PM, wrote: > > > > > Histonetters: > > > > In-office AP labs provide a valuable service to the practices they > serve > > by facilitating 1) better communication between pathologists and > ordering > > clinicians, 2) quality metrics that are practice-specific, and 3) > high > > volume, sub-specialization for both histotechnologists and > pathologists. > > In other words, the more of one type of histopathology a lab does > (e.g., > > skin, prostate, GI), the better it gets. Most people would not think > of > > having their cardiac bypass surgery done at a community hospital > doing > > 50/year; you want to go where more than 500/year are done. In > > histopathology, the kinds of volume you want are in the thousands for > each > > tissue type. Many hospital labs do little skin or prostate > histology > > anymore. Many sub-specialty in-office AP labs may do thousands of > cases of > > one tissue type every year. > > > > Aside from that, in-office AP labs are an emerging frontier of > employment > > for histologists and pathologists. In an era of high unemployment, > another > > source of employment for our professions is "a good thing." > > > > This requirement by an insurer for accreditation will help to > validate > > these in-office AP labs' commitment to quality and put them on the > level > > with their hospital counterparts. > > > > John D. Cochran, MD, FCAP > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From modz9636 <@t> gmail.com Mon Apr 9 19:22:15 2012 From: modz9636 <@t> gmail.com (M.O.) Date: Mon Apr 9 19:22:18 2012 Subject: [Histonet] New to paraffin cutting - seeking advice Message-ID: Hello everyone! I have had such wonderful feedback regarding plastic embedded specimens and now I am moving onto paraffin. The majority of the specimens I am going to be cutting are paraffin embedded decal rabbit femurs. I am using a Leica RM2255 and there is an option for retraction (after each section) and blade angle. I need to practice a lot because right now my sections are crinkly even though I have my samples on ice, but hopefully practice makes perfect! So I want to ask you all a few questions regarding settings and technique and one about H&E staining. What angle should the blade be at - I have it set at 0 for the moment. What about the retraction option - I have it set at 15um. The sections are being cut at 4um. When the blade has pieces of paraffin on it, do I need to clean that off? How do I remove this paraffin because I don't want to dull the blade? If not, does that impact the section quality and should I be moving to a different area of the blade? I am looking into flotation baths that are relatively inexpensive. In particular, I am looking for a simple bath with a glass pyrex dish. Do you have any suggestions on where to purchase one from? Lastly, I am going back to H&E staining of plastic embedded undecalcified bone, specifically hematoxylin, for a brief moment. When I use a clearing solution I know that I am trying to destain a bit from the undecalcified bone to make a lighter stain, but does this also significantly destain the nuclei? Right now, I need to leave the sample staining in Harris's hematoxylin for more than 4 mins to get the nuclei stained nicely, but I need to destain because the section is too dark. I just don't want to destain the nuclei too much, but want a lighter stain on the undecal bone. Again, thank you all for your support and advice, it is much appreciated! Sincerely, Merissa From abright <@t> brightinstruments.com Tue Apr 10 05:45:31 2012 From: abright <@t> brightinstruments.com (abright@brightinstruments.com) Date: Tue Apr 10 05:45:43 2012 Subject: [Histonet] HistoBath, HistoChill, Clini-RF In-Reply-To: References: Message-ID: <348837033-1334054733-cardhu_decombobulator_blackberry.rim.net-342062613-@b2.c2.bise7.blackberry> Dear Bob, I would just like to point out that the recommended freezing fluid for the Bright Clini-RF Rapid -80c tissue freezer is 3M's Novec HFE-7100 not 7000 as you state. Best regards Alan Bright www.brightinstruments.com Sent from my BlackBerry? wireless device -----Original Message----- From: Bob Richmond Sender: histonet-bounces@lists.utsouthwestern.edu Date: Mon, 9 Apr 2012 09:16:23 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoBath, HistoChill, Clini-RF Terri Bishop at SPScientific sent me an e-mail about HistoChill, a frozen section freezing bath that replaces the discontinued HistoBath. Terri didn't feel it was appropriate for a vendor to post this directly on HistoNet, so I am. You can contact Terri Bishop at Terri.Bishop@SPScientific.com HistoChill has been available for about a year. You can see the brochure at http://www.spscientific.com/Air-Stream-/-Baths-/-Chillers-/-Traps-/-Probes.aspx I'm pleased that they are specifically recommending using 3M's non-flammable perfluorocarbon HFE-7000 coolant, and not isopentane or acetone. (I feel like I've struck a blow for lab safety!) As has been noted on HistoNet before, Hacker Instruments offers Alan Bright's Clini-RF, a competing product. I have no commercial connection with any of the companies I've mentioned, and I have no personal experience with either instrument. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING-LINKS ------------------------------------------------------ Teach SpamSniper if this mail (ID 01GTNjypu) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01GTNjypu&m=00c557cef5d5&t=20120409&c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01GTNjypu&m=00c557cef5d5&t=20120409&c=n Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01GTNjypu&m=00c557cef5d5&t=20120409&c=f ------------------------------------------------------ END-ANTISPAM-VOTING-LINKS From SHUNTER <@t> beaumont.edu Tue Apr 10 06:38:12 2012 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Tue Apr 10 06:38:20 2012 Subject: [Histonet] HistoBath, HistoChill, Clini-RF In-Reply-To: <348837033-1334054733-cardhu_decombobulator_blackberry.rim.net-342062613-@b2.c2.bise7.blackberry> References: <348837033-1334054733-cardhu_decombobulator_blackberry.rim.net-342062613-@b2.c2.bise7.blackberry> Message-ID: Can you use the 3M freezing fluid in a histobath instead of isopentane? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of abright@brightinstruments.com Sent: Tuesday, April 10, 2012 6:46 AM To: Bob Richmond; histonet-bounces@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] HistoBath, HistoChill, Clini-RF Dear Bob, I would just like to point out that the recommended freezing fluid for the Bright Clini-RF Rapid -80c tissue freezer is 3M's Novec HFE-7100 not 7000 as you state. Best regards Alan Bright www.brightinstruments.com Sent from my BlackBerry(r) wireless device -----Original Message----- From: Bob Richmond Sender: histonet-bounces@lists.utsouthwestern.edu Date: Mon, 9 Apr 2012 09:16:23 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoBath, HistoChill, Clini-RF Terri Bishop at SPScientific sent me an e-mail about HistoChill, a frozen section freezing bath that replaces the discontinued HistoBath. Terri didn't feel it was appropriate for a vendor to post this directly on HistoNet, so I am. You can contact Terri Bishop at Terri.Bishop@SPScientific.com HistoChill has been available for about a year. You can see the brochure at http://www.spscientific.com/Air-Stream-/-Baths-/-Chillers-/-Traps-/-Probes.aspx I'm pleased that they are specifically recommending using 3M's non-flammable perfluorocarbon HFE-7000 coolant, and not isopentane or acetone. (I feel like I've struck a blow for lab safety!) As has been noted on HistoNet before, Hacker Instruments offers Alan Bright's Clini-RF, a competing product. I have no commercial connection with any of the companies I've mentioned, and I have no personal experience with either instrument. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING-LINKS ------------------------------------------------------ Teach SpamSniper if this mail (ID 01GTNjypu) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01GTNjypu&m=00c557cef5d5&t=20120409&c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01GTNjypu&m=00c557cef5d5&t=20120409&c=n Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01GTNjypu&m=00c557cef5d5&t=20120409&c=f ------------------------------------------------------ END-ANTISPAM-VOTING-LINKS This message has been scanned and no issues discovered. To report this email as SPAM, please forward it to spam@websense.com. From abright <@t> brightinstruments.com Tue Apr 10 08:29:16 2012 From: abright <@t> brightinstruments.com (abright@brightinstruments.com) Date: Tue Apr 10 08:29:27 2012 Subject: [Histonet] HistoBath, HistoChill, Clini-RF In-Reply-To: References: <348837033-1334054733-cardhu_decombobulator_blackberry.rim.net-342062613-@b2.c2.bise7.blackberry> Message-ID: <1490950558-1334064557-cardhu_decombobulator_blackberry.rim.net-1527003385-@b2.c2.bise7.blackberry> Dear Sue, Defiantly yes, it is much safer and more eco friendly. We supply a suitable dunking container with our Clin-RF as the specimens need to be contained to stop them floating in the 3M's fluid. Best regards Alan Bright www.brightinstruments.com Sent from my BlackBerry? wireless device -----Original Message----- From: Sue Hunter Date: Tue, 10 Apr 2012 11:38:12 To: abright@brightinstruments.com; Bob Richmond; histonet-bounces@lists.utsouthwestern.edu; Histonet Subject: RE: [Histonet] HistoBath, HistoChill, Clini-RF Can you use the 3M freezing fluid in a histobath instead of isopentane? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of abright@brightinstruments.com Sent: Tuesday, April 10, 2012 6:46 AM To: Bob Richmond; histonet-bounces@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] HistoBath, HistoChill, Clini-RF Dear Bob, I would just like to point out that the recommended freezing fluid for the Bright Clini-RF Rapid -80c tissue freezer is 3M's Novec HFE-7100 not 7000 as you state. Best regards Alan Bright www.brightinstruments.com Sent from my BlackBerry(r) wireless device -----Original Message----- From: Bob Richmond Sender: histonet-bounces@lists.utsouthwestern.edu Date: Mon, 9 Apr 2012 09:16:23 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoBath, HistoChill, Clini-RF Terri Bishop at SPScientific sent me an e-mail about HistoChill, a frozen section freezing bath that replaces the discontinued HistoBath. Terri didn't feel it was appropriate for a vendor to post this directly on HistoNet, so I am. You can contact Terri Bishop at Terri.Bishop@SPScientific.com HistoChill has been available for about a year. You can see the brochure at http://www.spscientific.com/Air-Stream-/-Baths-/-Chillers-/-Traps-/-Probes.aspx I'm pleased that they are specifically recommending using 3M's non-flammable perfluorocarbon HFE-7000 coolant, and not isopentane or acetone. (I feel like I've struck a blow for lab safety!) As has been noted on HistoNet before, Hacker Instruments offers Alan Bright's Clini-RF, a competing product. I have no commercial connection with any of the companies I've mentioned, and I have no personal experience with either instrument. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- This message has been scanned and no issues discovered. To report this email as SPAM, please forward it to spam@websense.com. -- BEGIN-ANTISPAM-VOTING-LINKS ------------------------------------------------------ Teach SpamSniper if this mail (ID 01GUbCehP) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01GUbCehP&m=134cc5b01928&t=20120410&c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01GUbCehP&m=134cc5b01928&t=20120410&c=n Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01GUbCehP&m=134cc5b01928&t=20120410&c=f ------------------------------------------------------ END-ANTISPAM-VOTING-LINKS From nicole <@t> dlcjax.com Tue Apr 10 08:45:06 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Tue Apr 10 08:45:13 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: References: <8CEE490B54C04D0-10D4-119D@webmail-m164.sysops.aol.com> <4F833BCA.7400.0077.1@harthosp.org> Message-ID: <1206.208.62.167.196.1334065506.squirrel@webmail.realpages.com> Money is at the root of all finicial decisions, in-house labs and hospitals. There are many over utilization of resources within the health care field. Many gallbladder surgerious are performed unneccesarly by general surgeous who's practice are within hospitals walls. Tonsilectomy. etc. How are those specimens not self reffered to the hospitals AP lab. David you made the comment about specialities staying with there specialty and not branching out. A dermatopathologist specializes in derm specimens so why is it so far fetched that he would read derm specimens from all sources, hospitals or in-house labs. My in-house lab has a higher turn around rate, lower overhead, and cuts courier fees out. We also do a service to our patients by allowing them one stop shopping. We can service all there needs and they do not have to have multiple appointments at different facilities. This cuts down on their copay and billing from multiple doctors. Also, it would cost more for a person to have Mohs surgery in a hospital setting. As we all know cost are higher at a hospital because they have higher overhead. The hospital is self reffering when they let a surgery center or group be affiliated with them. The surgery center was allowed to join the hospital so the hospital could reep the revenue generated and process their specimens. Either way, we are all joined by a common form of employment, and one facility is not better than another. My field provides jobs and creates revenue just like yours. Insurance company are going to make changes to try and make revenue during this change into "OBAMA CARE". Remeber we are not the enemy they are. Who are they to dictate how my company runs. Insurance companies have to much power and the decisions they force us to make do not always provide the best patient care. And that is the ultimate goal for any provider, to give best patient care right? This is just another hurdle we all must jump through in these comming changes. I vote we stick together and try our best to protect all our jobs. Wasnt that long ago that each of us we trying to get pay increases and bring the importance of our jobs to the fore front of pathology. The financial squeeze of the helath care system is going to be felt by all. Histology, pathology, radiology, cytology, we all must do our best to role with the punches and ensure quality care and our incomes, as well as our field, reguardless of location. Nicole Tatum, HT ASCP Thank you for that. How are things at Hartford Hospital? One of my > favorite > places, rotated there many years ago. Very impressive facility! Is Dr. > Ricci still there? > On Mon, Apr 9, 2012 at 4:43 PM, Richard Cartun > wrote: > >> This was released today. >> >> Richard >> >> Statline Special Alert: >> New Evidence Links Self-Referral Labs to Increased Utilization, Lower >> Cancer Detection Rates >> Study co-funded by CAP Appears in April 2012 Issue of Health Affairs >> April 9?Self-referring urologists billed Medicare for nearly 75% more >> anatomic pathology (AP) specimens compared to non self-referring >> physicians, according to a study published today in a leading health >> care policy journal. Furthermore, the study found no increase in cancer >> detection for the patients of self-referring physicians-in fact, the >> detection rate was 14% lower than that of non self-referring >> physicians. >> >> These findings, from an independent study co-funded by the CAP, provide >> the first clear evidence that self-referral of anatomic pathology >> services leads to increased utilization, higher Medicare spending, and >> lower rates of cancer detection. The study, led by renowned Georgetown >> University health care economist Jean Mitchell, PhD, will appear in the >> April 2012 issue of Health Affairs and is now available on the >> journal?s website. >> >> >> -------------------------------------------------------------------------------- >> >> >> >>> Daniel Schneider 4/9/2012 4:47 PM >>> >> This is all about the money. The rest is rationalization. >> >> The reason a group of non-pathologist physicians opens an in-house >> pathology lab and hires an employee pathologist is first and foremost >> to harvest profit from pathology reimbursement. Be a fly on the wall in >> the >> partners' meetings and you would know that's what they are talking >> about. >> >> To suggest otherwise is disingenuous. >> >> And the implication that the generalist anatomic pathologist is >> unqualified >> to be signing out skins, prostates, GI's and whatever is >> reprehensible. >> This is not cardiac bypass surgery, and AP pathologists *are* trained >> to do >> all of the above. I eagerly defer to subspecialty expert consultants >> as >> needed, but most of the time they're not needed. >> >> Hospital labs that see few, if any skins, prostates, GI's, are only in >> that >> pickle because of the cherrypicking they've already been subjected to. >> >> *"in-office AP labs are an emerging frontier of employment for >> histologists >> and pathologists. In an era of high unemployment, another source of >> employment for our professions is "a good thing.""* >> >> Really? The jobs follow the specimens. Given the same number of >> specimens, >> there's the same number of jobs, more or less, just under different >> circumstances and in different locations Unless you're suggesting >> that >> in-office labs will generate increased specimens, and thus increased >> jobs >> though overutilization, i.e. excessive numbers of unnecessary biopsies >> and >> abuse of the patient and the taxpayer. In which case I have to say >> there's >> a grain of truth. And the truth hurts. And it's not " a good thing." >> >> None of this should be taken as criticism of histotechs and >> pathologists >> who find themselves working in an in-office lab. I know there's bills >> to >> pay, families to take care of, and god knows it's hard for a >> pathologist to >> find a job these days with the numbers our residency programs keep >> churning >> out (but that's another rant...). >> >> Dan Schneider, MD >> Amarillo, TX >> >> >> >> >> >> >> >> >> >> >> >> >> On Mon, Apr 9, 2012 at 12:52 PM, wrote: >> >> > >> > Histonetters: >> > >> > In-office AP labs provide a valuable service to the practices they >> serve >> > by facilitating 1) better communication between pathologists and >> ordering >> > clinicians, 2) quality metrics that are practice-specific, and 3) >> high >> > volume, sub-specialization for both histotechnologists and >> pathologists. >> > In other words, the more of one type of histopathology a lab does >> (e.g., >> > skin, prostate, GI), the better it gets. Most people would not think >> of >> > having their cardiac bypass surgery done at a community hospital >> doing >> > 50/year; you want to go where more than 500/year are done. In >> > histopathology, the kinds of volume you want are in the thousands for >> each >> > tissue type. Many hospital labs do little skin or prostate >> histology >> > anymore. Many sub-specialty in-office AP labs may do thousands of >> cases of >> > one tissue type every year. >> > >> > Aside from that, in-office AP labs are an emerging frontier of >> employment >> > for histologists and pathologists. In an era of high unemployment, >> another >> > source of employment for our professions is "a good thing." >> > >> > This requirement by an insurer for accreditation will help to >> validate >> > these in-office AP labs' commitment to quality and put them on the >> level >> > with their hospital counterparts. >> > >> > John D. Cochran, MD, FCAP >> > >> > >> > >> > >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Caroline.Pratt <@t> uphs.upenn.edu Tue Apr 10 08:47:33 2012 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Tue Apr 10 08:47:22 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: <19B9AB11-C858-4ED0-99CE-EF4939B448A5@yahoo.com> References: <8CEE490B54C04D0-10D4-119D@webmail-m164.sysops.aol.com> <19B9AB11-C858-4ED0-99CE-EF4939B448A5@yahoo.com> Message-ID: There are pros and cons to both business structures. I love the information I get on histonet, but why does everything have to turn into an argument? Can't we just respect each other's opinions? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Monday, April 09, 2012 5:58 PM To: Daniel Schneider Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation To suggest that any physician who goes into private practice and has their own lab is any more of a money hound than any other physician at a hospital would also be disingenuous . And of course this is about money. It's about one group of people trying to get another group of people out of the lab business because they want that money. It's also about the government squeezing insurance companies into these more stringent regulations. Now I'm not against more stringent regulations but I do find it offensive of how they are going about it. The little guy will take the hits on this one. I guess what they want is a bunch of walmart like labs. Private practices serve a patient care cause just as hospital labs do. They all make a diagnosis. They all deserve to be paid. My 2 cents Sent from my iPhone On Apr 9, 2012, at 4:47 PM, Daniel Schneider wrote: > This is all about the money. The rest is rationalization. > > The reason a group of non-pathologist physicians opens an in-house > pathology lab and hires an employee pathologist is first and foremost > to harvest profit from pathology reimbursement. Be a fly on the wall in the > partners' meetings and you would know that's what they are talking about. > > To suggest otherwise is disingenuous. > > And the implication that the generalist anatomic pathologist is unqualified > to be signing out skins, prostates, GI's and whatever is reprehensible. > This is not cardiac bypass surgery, and AP pathologists *are* trained to do > all of the above. I eagerly defer to subspecialty expert consultants as > needed, but most of the time they're not needed. > > Hospital labs that see few, if any skins, prostates, GI's, are only in that > pickle because of the cherrypicking they've already been subjected to. > > *"in-office AP labs are an emerging frontier of employment for histologists > and pathologists. In an era of high unemployment, another source of > employment for our professions is "a good thing.""* > > Really? The jobs follow the specimens. Given the same number of specimens, > there's the same number of jobs, more or less, just under different > circumstances and in different locations Unless you're suggesting that > in-office labs will generate increased specimens, and thus increased jobs > though overutilization, i.e. excessive numbers of unnecessary biopsies and > abuse of the patient and the taxpayer. In which case I have to say there's > a grain of truth. And the truth hurts. And it's not " a good thing." > > None of this should be taken as criticism of histotechs and pathologists > who find themselves working in an in-office lab. I know there's bills to > pay, families to take care of, and god knows it's hard for a pathologist to > find a job these days with the numbers our residency programs keep churning > out (but that's another rant...). > > Dan Schneider, MD > Amarillo, TX > > > > > > > > > > > > > On Mon, Apr 9, 2012 at 12:52 PM, wrote: > >> >> Histonetters: >> >> In-office AP labs provide a valuable service to the practices they serve >> by facilitating 1) better communication between pathologists and ordering >> clinicians, 2) quality metrics that are practice-specific, and 3) high >> volume, sub-specialization for both histotechnologists and pathologists. >> In other words, the more of one type of histopathology a lab does (e.g., >> skin, prostate, GI), the better it gets. Most people would not think of >> having their cardiac bypass surgery done at a community hospital doing >> 50/year; you want to go where more than 500/year are done. In >> histopathology, the kinds of volume you want are in the thousands for each >> tissue type. Many hospital labs do little skin or prostate histology >> anymore. Many sub-specialty in-office AP labs may do thousands of cases of >> one tissue type every year. >> >> Aside from that, in-office AP labs are an emerging frontier of employment >> for histologists and pathologists. In an era of high unemployment, another >> source of employment for our professions is "a good thing." >> >> This requirement by an insurer for accreditation will help to validate >> these in-office AP labs' commitment to quality and put them on the level >> with their hospital counterparts. >> >> John D. Cochran, MD, FCAP >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From nicole <@t> dlcjax.com Tue Apr 10 08:51:19 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Tue Apr 10 08:51:24 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: <19B9AB11-C858-4ED0-99CE-EF4939B448A5@yahoo.com> References: <8CEE490B54C04D0-10D4-119D@webmail-m164.sysops.aol.com> <19B9AB11-C858-4ED0-99CE-EF4939B448A5@yahoo.com> Message-ID: <1235.208.62.167.196.1334065879.squirrel@webmail.realpages.com> Well Said To suggest that any physician who goes into private practice and has their > own lab is any more of a money hound than any other physician at a > hospital would also be disingenuous . And of course this is about money. > It's about one group of people trying to get another group of people out > of the lab business because they want that money. It's also about the > government squeezing insurance companies into these more stringent > regulations. Now I'm not against more stringent regulations but I do find > it offensive of how they are going about it. The little guy will take the > hits on this one. I guess what they want is a bunch of walmart like labs. > Private practices serve a patient care cause just as hospital labs do. > They all make a diagnosis. They all deserve to be paid. > > My 2 cents > > Sent from my iPhone > > On Apr 9, 2012, at 4:47 PM, Daniel Schneider > wrote: > >> This is all about the money. The rest is rationalization. >> >> The reason a group of non-pathologist physicians opens an in-house >> pathology lab and hires an employee pathologist is first and foremost >> to harvest profit from pathology reimbursement. Be a fly on the wall in >> the >> partners' meetings and you would know that's what they are talking >> about. >> >> To suggest otherwise is disingenuous. >> >> And the implication that the generalist anatomic pathologist is >> unqualified >> to be signing out skins, prostates, GI's and whatever is reprehensible. >> This is not cardiac bypass surgery, and AP pathologists *are* trained to >> do >> all of the above. I eagerly defer to subspecialty expert consultants as >> needed, but most of the time they're not needed. >> >> Hospital labs that see few, if any skins, prostates, GI's, are only in >> that >> pickle because of the cherrypicking they've already been subjected to. >> >> *"in-office AP labs are an emerging frontier of employment for >> histologists >> and pathologists. In an era of high unemployment, another source of >> employment for our professions is "a good thing.""* >> >> Really? The jobs follow the specimens. Given the same number of >> specimens, >> there's the same number of jobs, more or less, just under different >> circumstances and in different locations Unless you're suggesting that >> in-office labs will generate increased specimens, and thus increased >> jobs >> though overutilization, i.e. excessive numbers of unnecessary biopsies >> and >> abuse of the patient and the taxpayer. In which case I have to say >> there's >> a grain of truth. And the truth hurts. And it's not " a good thing." >> >> None of this should be taken as criticism of histotechs and pathologists >> who find themselves working in an in-office lab. I know there's bills to >> pay, families to take care of, and god knows it's hard for a pathologist >> to >> find a job these days with the numbers our residency programs keep >> churning >> out (but that's another rant...). >> >> Dan Schneider, MD >> Amarillo, TX >> >> >> >> >> >> >> >> >> >> >> >> >> On Mon, Apr 9, 2012 at 12:52 PM, wrote: >> >>> >>> Histonetters: >>> >>> In-office AP labs provide a valuable service to the practices they >>> serve >>> by facilitating 1) better communication between pathologists and >>> ordering >>> clinicians, 2) quality metrics that are practice-specific, and 3) high >>> volume, sub-specialization for both histotechnologists and >>> pathologists. >>> In other words, the more of one type of histopathology a lab does >>> (e.g., >>> skin, prostate, GI), the better it gets. Most people would not think >>> of >>> having their cardiac bypass surgery done at a community hospital doing >>> 50/year; you want to go where more than 500/year are done. In >>> histopathology, the kinds of volume you want are in the thousands for >>> each >>> tissue type. Many hospital labs do little skin or prostate histology >>> anymore. Many sub-specialty in-office AP labs may do thousands of >>> cases of >>> one tissue type every year. >>> >>> Aside from that, in-office AP labs are an emerging frontier of >>> employment >>> for histologists and pathologists. In an era of high unemployment, >>> another >>> source of employment for our professions is "a good thing." >>> >>> This requirement by an insurer for accreditation will help to validate >>> these in-office AP labs' commitment to quality and put them on the >>> level >>> with their hospital counterparts. >>> >>> John D. Cochran, MD, FCAP >>> >>> >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond <@t> gmail.com Tue Apr 10 08:59:06 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Apr 10 08:59:12 2012 Subject: [Histonet] Re: HistoBath, HistoChill, Clini-RF Message-ID: Sue Hunter asks: >>Can you use the 3M freezing fluid in a HistoBath instead of isopentane?<< I haven't seen it done, but I understand that the 3M freezing fluid can be used in the old HistoBath, if you still have one of them. Alan Bright pointed out something I didn't know - that because of the greater density of the 3M freezing liquid, specimens float in it. Bob Richmond Samurai Pathologist Knoxville TN From dlschneider <@t> gmail.com Tue Apr 10 09:16:24 2012 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Tue Apr 10 09:16:32 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: References: <8CEE490B54C04D0-10D4-119D@webmail-m164.sysops.aol.com> <19B9AB11-C858-4ED0-99CE-EF4939B448A5@yahoo.com> Message-ID: Because some things are worth arguing about or fighting for? That there are two sides to a conflict doesn't imply that the sides are equally right. It's well documented that clinicians who own their own pathology labs, and profit from the processing and reading of their biopsies, generate significantly more biopsies. Is that good for the patient? Incentives matter. Separating the biopsy grabbing from the biopsy processing/reading is one small way to remove an incentive to abuse the patient and the taxpayer. On Tue, Apr 10, 2012 at 8:47 AM, Pratt, Caroline < Caroline.Pratt@uphs.upenn.edu> wrote: > There are pros and cons to both business structures. I love the > information I get on histonet, but why does everything have to turn into > an argument? Can't we just respect each other's opinions? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Donadio > Sent: Monday, April 09, 2012 5:58 PM > To: Daniel Schneider > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation > > To suggest that any physician who goes into private practice and has > their own lab is any more of a money hound than any other physician at a > hospital would also be disingenuous . And of course this is about money. > It's about one group of people trying to get another group of people out > of the lab business because they want that money. It's also about the > government squeezing insurance companies into these more stringent > regulations. Now I'm not against more stringent regulations but I do > find it offensive of how they are going about it. The little guy will > take the hits on this one. I guess what they want is a bunch of walmart > like labs. Private practices serve a patient care cause just as hospital > labs do. They all make a diagnosis. They all deserve to be paid. > > My 2 cents > > Sent from my iPhone > > On Apr 9, 2012, at 4:47 PM, Daniel Schneider > wrote: > > > This is all about the money. The rest is rationalization. > > > > The reason a group of non-pathologist physicians opens an in-house > > pathology lab and hires an employee pathologist is first and foremost > > to harvest profit from pathology reimbursement. Be a fly on the wall > in the > > partners' meetings and you would know that's what they are talking > about. > > > > To suggest otherwise is disingenuous. > > > > And the implication that the generalist anatomic pathologist is > unqualified > > to be signing out skins, prostates, GI's and whatever is > reprehensible. > > This is not cardiac bypass surgery, and AP pathologists *are* trained > to do > > all of the above. I eagerly defer to subspecialty expert consultants > as > > needed, but most of the time they're not needed. > > > > Hospital labs that see few, if any skins, prostates, GI's, are only in > that > > pickle because of the cherrypicking they've already been subjected to. > > > > *"in-office AP labs are an emerging frontier of employment for > histologists > > and pathologists. In an era of high unemployment, another source of > > employment for our professions is "a good thing.""* > > > > Really? The jobs follow the specimens. Given the same number of > specimens, > > there's the same number of jobs, more or less, just under different > > circumstances and in different locations Unless you're suggesting > that > > in-office labs will generate increased specimens, and thus increased > jobs > > though overutilization, i.e. excessive numbers of unnecessary biopsies > and > > abuse of the patient and the taxpayer. In which case I have to say > there's > > a grain of truth. And the truth hurts. And it's not " a good thing." > > > > None of this should be taken as criticism of histotechs and > pathologists > > who find themselves working in an in-office lab. I know there's bills > to > > pay, families to take care of, and god knows it's hard for a > pathologist to > > find a job these days with the numbers our residency programs keep > churning > > out (but that's another rant...). > > > > Dan Schneider, MD > > Amarillo, TX > > > > > > > > > > > > > > > > > > > > > > > > > > On Mon, Apr 9, 2012 at 12:52 PM, wrote: > > > >> > >> Histonetters: > >> > >> In-office AP labs provide a valuable service to the practices they > serve > >> by facilitating 1) better communication between pathologists and > ordering > >> clinicians, 2) quality metrics that are practice-specific, and 3) > high > >> volume, sub-specialization for both histotechnologists and > pathologists. > >> In other words, the more of one type of histopathology a lab does > (e.g., > >> skin, prostate, GI), the better it gets. Most people would not think > of > >> having their cardiac bypass surgery done at a community hospital > doing > >> 50/year; you want to go where more than 500/year are done. In > >> histopathology, the kinds of volume you want are in the thousands for > each > >> tissue type. Many hospital labs do little skin or prostate histology > >> anymore. Many sub-specialty in-office AP labs may do thousands of > cases of > >> one tissue type every year. > >> > >> Aside from that, in-office AP labs are an emerging frontier of > employment > >> for histologists and pathologists. In an era of high unemployment, > another > >> source of employment for our professions is "a good thing." > >> > >> This requirement by an insurer for accreditation will help to > validate > >> these in-office AP labs' commitment to quality and put them on the > level > >> with their hospital counterparts. > >> > >> John D. Cochran, MD, FCAP > >> > >> > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this e-mail message is intended only for the > personal and confidential use of the recipient(s) named above. If the > reader of this message is not the intended recipient or an agent > responsible for delivering it to the intended recipient, you are hereby > notified that you have received this document in error and that any review, > dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please notify > us immediately by e-mail, and delete the original message. > From pathlocums <@t> gmail.com Tue Apr 10 09:16:36 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Tue Apr 10 09:16:40 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation Message-ID: <-949456142732028855@unknownmsgid> Start with reading Dr. Schneider's post. Then read Richard Cartun's post. Those should deal will what you are talking about very well. These in-office labs should not exist, for the very same reason the undertaker is no longer the ambulance driver. There is a very real, and significant conflict of interest. Sent from my Windows Phone From: Nicole Tatum Sent: 4/10/2012 6:45 AM To: Davide Costanzo; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation Money is at the root of all finicial decisions, in-house labs and hospitals. There are many over utilization of resources within the health care field. Many gallbladder surgerious are performed unneccesarly by general surgeous who's practice are within hospitals walls. Tonsilectomy. etc. How are those specimens not self reffered to the hospitals AP lab. David you made the comment about specialities staying with there specialty and not branching out. A dermatopathologist specializes in derm specimens so why is it so far fetched that he would read derm specimens from all sources, hospitals or in-house labs. My in-house lab has a higher turn around rate, lower overhead, and cuts courier fees out. We also do a service to our patients by allowing them one stop shopping. We can service all there needs and they do not have to have multiple appointments at different facilities. This cuts down on their copay and billing from multiple doctors. Also, it would cost more for a person to have Mohs surgery in a hospital setting. As we all know cost are higher at a hospital because they have higher overhead. The hospital is self reffering when they let a surgery center or group be affiliated with them. The surgery center was allowed to join the hospital so the hospital could reep the revenue generated and process their specimens. Either way, we are all joined by a common form of employment, and one facility is not better than another. My field provides jobs and creates revenue just like yours. Insurance company are going to make changes to try and make revenue during this change into "OBAMA CARE". Remeber we are not the enemy they are. Who are they to dictate how my company runs. Insurance companies have to much power and the decisions they force us to make do not always provide the best patient care. And that is the ultimate goal for any provider, to give best patient care right? This is just another hurdle we all must jump through in these comming changes. I vote we stick together and try our best to protect all our jobs. Wasnt that long ago that each of us we trying to get pay increases and bring the importance of our jobs to the fore front of pathology. The financial squeeze of the helath care system is going to be felt by all. Histology, pathology, radiology, cytology, we all must do our best to role with the punches and ensure quality care and our incomes, as well as our field, reguardless of location. Nicole Tatum, HT ASCP Thank you for that. How are things at Hartford Hospital? One of my > favorite > places, rotated there many years ago. Very impressive facility! Is Dr. > Ricci still there? > On Mon, Apr 9, 2012 at 4:43 PM, Richard Cartun > wrote: > >> This was released today. >> >> Richard >> >> Statline Special Alert: >> New Evidence Links Self-Referral Labs to Increased Utilization, Lower >> Cancer Detection Rates >> Study co-funded by CAP Appears in April 2012 Issue of Health Affairs >> April 9?Self-referring urologists billed Medicare for nearly 75% more >> anatomic pathology (AP) specimens compared to non self-referring >> physicians, according to a study published today in a leading health >> care policy journal. Furthermore, the study found no increase in cancer >> detection for the patients of self-referring physicians-in fact, the >> detection rate was 14% lower than that of non self-referring >> physicians. >> >> These findings, from an independent study co-funded by the CAP, provide >> the first clear evidence that self-referral of anatomic pathology >> services leads to increased utilization, higher Medicare spending, and >> lower rates of cancer detection. The study, led by renowned Georgetown >> University health care economist Jean Mitchell, PhD, will appear in the >> April 2012 issue of Health Affairs and is now available on the >> journal?s website. >> >> >> -------------------------------------------------------------------------------- >> >> >> >>> Daniel Schneider 4/9/2012 4:47 PM >>> >> This is all about the money. The rest is rationalization. >> >> The reason a group of non-pathologist physicians opens an in-house >> pathology lab and hires an employee pathologist is first and foremost >> to harvest profit from pathology reimbursement. Be a fly on the wall in >> the >> partners' meetings and you would know that's what they are talking >> about. >> >> To suggest otherwise is disingenuous. >> >> And the implication that the generalist anatomic pathologist is >> unqualified >> to be signing out skins, prostates, GI's and whatever is >> reprehensible. >> This is not cardiac bypass surgery, and AP pathologists *are* trained >> to do >> all of the above. I eagerly defer to subspecialty expert consultants >> as >> needed, but most of the time they're not needed. >> >> Hospital labs that see few, if any skins, prostates, GI's, are only in >> that >> pickle because of the cherrypicking they've already been subjected to. >> >> *"in-office AP labs are an emerging frontier of employment for >> histologists >> and pathologists. In an era of high unemployment, another source of >> employment for our professions is "a good thing.""* >> >> Really? The jobs follow the specimens. Given the same number of >> specimens, >> there's the same number of jobs, more or less, just under different >> circumstances and in different locations Unless you're suggesting >> that >> in-office labs will generate increased specimens, and thus increased >> jobs >> though overutilization, i.e. excessive numbers of unnecessary biopsies >> and >> abuse of the patient and the taxpayer. In which case I have to say >> there's >> a grain of truth. And the truth hurts. And it's not " a good thing." >> >> None of this should be taken as criticism of histotechs and >> pathologists >> who find themselves working in an in-office lab. I know there's bills >> to >> pay, families to take care of, and god knows it's hard for a >> pathologist to >> find a job these days with the numbers our residency programs keep >> churning >> out (but that's another rant...). >> >> Dan Schneider, MD >> Amarillo, TX >> >> >> >> >> >> >> >> >> >> >> >> >> On Mon, Apr 9, 2012 at 12:52 PM, wrote: >> >> > >> > Histonetters: >> > >> > In-office AP labs provide a valuable service to the practices they >> serve >> > by facilitating 1) better communication between pathologists and >> ordering >> > clinicians, 2) quality metrics that are practice-specific, and 3) >> high >> > volume, sub-specialization for both histotechnologists and >> pathologists. >> > In other words, the more of one type of histopathology a lab does >> (e.g., >> > skin, prostate, GI), the better it gets. Most people would not think >> of >> > having their cardiac bypass surgery done at a community hospital >> doing >> > 50/year; you want to go where more than 500/year are done. In >> > histopathology, the kinds of volume you want are in the thousands for >> each >> > tissue type. Many hospital labs do little skin or prostate >> histology >> > anymore. Many sub-specialty in-office AP labs may do thousands of >> cases of >> > one tissue type every year. >> > >> > Aside from that, in-office AP labs are an emerging frontier of >> employment >> > for histologists and pathologists. In an era of high unemployment, >> another >> > source of employment for our professions is "a good thing." >> > >> > This requirement by an insurer for accreditation will help to >> validate >> > these in-office AP labs' commitment to quality and put them on the >> level >> > with their hospital counterparts. >> > >> > John D. Cochran, MD, FCAP >> > >> > >> > >> > >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Erin.Martin <@t> ucsf.edu Tue Apr 10 09:38:07 2012 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Tue Apr 10 09:38:18 2012 Subject: [Histonet] Microwave processors Message-ID: <24B7B291CC88D04AB663958E77A1F59D02E3D6@ex09.net.ucsf.edu> Hi histonetters! Our pathologists want to turn around skin biopsies same day and are again looking at microwave processors. Due to a bad past experience, I'm not enthused but perhaps there is someone out there who loves their microwave processor? Even on derm? Or has anyone worked out a good rapid derm processing protocol on a conventional processor? Thank you so much! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any actin in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From brannon <@t> alliedsearchpartners.com Tue Apr 10 10:09:13 2012 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Tue Apr 10 10:09:43 2012 Subject: [Histonet] Current Histology Openings Message-ID: Allied Search Partners is looking for qualified histology professionals to fill the below positions. Interested candidates should forward an updated resume to brannon@alliedsearchpartners.com for a full job description and consideration for hire. All the below positions are for permanent placement and direct hire. 1) Histology Manager- Ft. Myers, FL 2) Immunospecialist- Tyler, TX 3) Histotechnician or Histotechnologist- Naples, FL 4) Histotechnologist (Lead)- Fort Myers, FL 5) Histotechnician or Histotechnologist- Knoxville, TN 6) Histotechnician or Histotechnologist- Port Chester, NY 7) Histotechnician or Histotechnologist- Denver, CO 8) Mohs Technician- Denver, CO 9) Histotechnician or Histotechnologist (part time)- Portland, OR -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From nicole <@t> dlcjax.com Tue Apr 10 10:18:19 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Tue Apr 10 10:18:25 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: <-949456142732028855@unknownmsgid> References: <-949456142732028855@unknownmsgid> Message-ID: <1370.208.62.167.196.1334071099.squirrel@webmail.realpages.com> Really, An undertaker. Yea, theres definately a conflict here, you. No since in wasting my time. Nicole Start with reading Dr. Schneider's post. Then read Richard Cartun's > post. Those should deal will what you are talking about very well. > > These in-office labs should not exist, for the very same reason the > undertaker is no longer the ambulance driver. There is a very real, and > significant conflict of interest. > > Sent from my Windows Phone > From: Nicole Tatum > Sent: 4/10/2012 6:45 AM > To: Davide Costanzo; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation > Money is at the root of all finicial decisions, in-house labs and > hospitals. There are many over utilization of resources within the health > care field. Many gallbladder surgerious are performed unneccesarly by > general surgeous who's practice are within hospitals walls. Tonsilectomy. > etc. How are those specimens not self reffered to the hospitals AP lab. > David you made the comment about specialities staying with there specialty > and not branching out. A dermatopathologist specializes in derm specimens > so why is it so far fetched that he would read derm specimens from all > sources, hospitals or in-house labs. My in-house lab has a higher turn > around rate, lower overhead, and cuts courier fees out. We also do a > service to our patients by allowing them one stop shopping. We can service > all there needs and they do not have to have multiple appointments at > different facilities. This cuts down on their copay and billing from > multiple doctors. Also, it would cost more for a person to have Mohs > surgery in a hospital setting. As we all know cost are higher at a > hospital because they have higher overhead. The hospital is self reffering > when they let a surgery center or group be affiliated with them. The > surgery center was allowed to join the hospital so the hospital could reep > the revenue generated and process their specimens. Either way, we are all > joined by a common form of employment, and one facility is not better than > another. My field provides jobs and creates revenue just like yours. > Insurance company are going to make changes to try and make revenue during > this change into "OBAMA CARE". Remeber we are not the enemy they are. Who > are they to dictate how my company runs. Insurance companies have to much > power and the decisions they force us to make do not always provide the > best patient care. And that is the ultimate goal for any provider, to give > best patient care right? This is just another hurdle we all must jump > through in these comming changes. I vote we stick together and try our > best to protect all our jobs. Wasnt that long ago that each of us we > trying to get pay increases and bring the importance of our jobs to the > fore front of pathology. The financial squeeze of the helath care system > is going to be felt by all. Histology, pathology, radiology, cytology, we > all must do our best to role with the punches and ensure quality care and > our incomes, as well as our field, reguardless of location. > > Nicole Tatum, HT ASCP > > > > > > Thank you for that. How are things at Hartford Hospital? One of my >> favorite >> places, rotated there many years ago. Very impressive facility! Is Dr. >> Ricci still there? >> On Mon, Apr 9, 2012 at 4:43 PM, Richard Cartun >> wrote: >> >>> This was released today. >>> >>> Richard >>> >>> Statline Special Alert: >>> New Evidence Links Self-Referral Labs to Increased Utilization, Lower >>> Cancer Detection Rates >>> Study co-funded by CAP Appears in April 2012 Issue of Health Affairs >>> April 9?Self-referring urologists billed Medicare for nearly 75% more >>> anatomic pathology (AP) specimens compared to non self-referring >>> physicians, according to a study published today in a leading health >>> care policy journal. Furthermore, the study found no increase in cancer >>> detection for the patients of self-referring physicians-in fact, the >>> detection rate was 14% lower than that of non self-referring >>> physicians. >>> >>> These findings, from an independent study co-funded by the CAP, provide >>> the first clear evidence that self-referral of anatomic pathology >>> services leads to increased utilization, higher Medicare spending, and >>> lower rates of cancer detection. The study, led by renowned Georgetown >>> University health care economist Jean Mitchell, PhD, will appear in the >>> April 2012 issue of Health Affairs and is now available on the >>> journal?s website. >>> >>> >>> -------------------------------------------------------------------------------- >>> >>> >>> >>> Daniel Schneider 4/9/2012 4:47 PM >>> >>> This is all about the money. The rest is rationalization. >>> >>> The reason a group of non-pathologist physicians opens an in-house >>> pathology lab and hires an employee pathologist is first and foremost >>> to harvest profit from pathology reimbursement. Be a fly on the wall in >>> the >>> partners' meetings and you would know that's what they are talking >>> about. >>> >>> To suggest otherwise is disingenuous. >>> >>> And the implication that the generalist anatomic pathologist is >>> unqualified >>> to be signing out skins, prostates, GI's and whatever is >>> reprehensible. >>> This is not cardiac bypass surgery, and AP pathologists *are* trained >>> to do >>> all of the above. I eagerly defer to subspecialty expert consultants >>> as >>> needed, but most of the time they're not needed. >>> >>> Hospital labs that see few, if any skins, prostates, GI's, are only in >>> that >>> pickle because of the cherrypicking they've already been subjected to. >>> >>> *"in-office AP labs are an emerging frontier of employment for >>> histologists >>> and pathologists. In an era of high unemployment, another source of >>> employment for our professions is "a good thing.""* >>> >>> Really? The jobs follow the specimens. Given the same number of >>> specimens, >>> there's the same number of jobs, more or less, just under different >>> circumstances and in different locations Unless you're suggesting >>> that >>> in-office labs will generate increased specimens, and thus increased >>> jobs >>> though overutilization, i.e. excessive numbers of unnecessary biopsies >>> and >>> abuse of the patient and the taxpayer. In which case I have to say >>> there's >>> a grain of truth. And the truth hurts. And it's not " a good thing." >>> >>> None of this should be taken as criticism of histotechs and >>> pathologists >>> who find themselves working in an in-office lab. I know there's bills >>> to >>> pay, families to take care of, and god knows it's hard for a >>> pathologist to >>> find a job these days with the numbers our residency programs keep >>> churning >>> out (but that's another rant...). >>> >>> Dan Schneider, MD >>> Amarillo, TX >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> On Mon, Apr 9, 2012 at 12:52 PM, wrote: >>> >>> > >>> > Histonetters: >>> > >>> > In-office AP labs provide a valuable service to the practices they >>> serve >>> > by facilitating 1) better communication between pathologists and >>> ordering >>> > clinicians, 2) quality metrics that are practice-specific, and 3) >>> high >>> > volume, sub-specialization for both histotechnologists and >>> pathologists. >>> > In other words, the more of one type of histopathology a lab does >>> (e.g., >>> > skin, prostate, GI), the better it gets. Most people would not think >>> of >>> > having their cardiac bypass surgery done at a community hospital >>> doing >>> > 50/year; you want to go where more than 500/year are done. In >>> > histopathology, the kinds of volume you want are in the thousands for >>> each >>> > tissue type. Many hospital labs do little skin or prostate >>> histology >>> > anymore. Many sub-specialty in-office AP labs may do thousands of >>> cases of >>> > one tissue type every year. >>> > >>> > Aside from that, in-office AP labs are an emerging frontier of >>> employment >>> > for histologists and pathologists. In an era of high unemployment, >>> another >>> > source of employment for our professions is "a good thing." >>> > >>> > This requirement by an insurer for accreditation will help to >>> validate >>> > these in-office AP labs' commitment to quality and put them on the >>> level >>> > with their hospital counterparts. >>> > >>> > John D. Cochran, MD, FCAP >>> > >>> > >>> > >>> > >>> > >>> > _______________________________________________ >>> > Histonet mailing list >>> > Histonet@lists.utsouthwestern.edu >>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> -- >> *David Costanzo, MHS, PA (ASCP)* >> Project Manager >> *Blufrog Path Lab Solutions* >> 9401 Wilshire Blvd. Ste 650 >> Beverly Hills, CA 90212 >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From contact <@t> excaliburpathology.com Tue Apr 10 10:26:07 2012 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Apr 10 10:26:49 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: <1370.208.62.167.196.1334071099.squirrel@webmail.realpages.com> References: <-949456142732028855@unknownmsgid> <1370.208.62.167.196.1334071099.squirrel@webmail.realpages.com> Message-ID: <1334071567.10711.YahooMailNeo@web5708.biz.mail.ne1.yahoo.com> Ditto Nicole! My daughter just passed her FUNERAL DIRECTOR boards! First time, I might add. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Nicole Tatum To: Davide Costanzo ; histonet@lists.utsouthwestern.edu Sent: Tuesday, April 10, 2012 10:18 AM Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation Really, An undertaker. Yea, theres definately a conflict here, you. No since in wasting my time. Nicole Start with reading Dr. Schneider's post. Then read Richard Cartun's > post. Those should deal will what you are talking about very well. > > These in-office labs should not exist, for the very same reason the > undertaker is no longer the ambulance driver. There is a very real, and > significant conflict of interest. > > Sent from my Windows Phone > From: Nicole Tatum > Sent: 4/10/2012 6:45 AM > To: Davide Costanzo; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation > Money is at the root of all finicial decisions, in-house labs and > hospitals. There are many over utilization of resources within the health > care field. Many gallbladder surgerious are performed unneccesarly by > general surgeous who's practice are within hospitals walls. Tonsilectomy. > etc. How are those specimens not self reffered to the hospitals AP lab. > David you made the comment about specialities staying with there specialty > and not branching out. A dermatopathologist specializes in derm specimens > so why is it so far fetched that he would read derm specimens from all > sources, hospitals or in-house labs. My in-house lab has a higher turn > around rate, lower overhead, and cuts courier fees out. We also do a > service to our patients by allowing them one stop shopping. We can service > all there needs and they do not have to have multiple appointments at > different facilities. This cuts down on their copay and billing from > multiple doctors. Also, it would cost more for a person to have Mohs > surgery in a hospital setting. As we all know cost are higher at a > hospital because they have higher overhead. The hospital is self reffering > when they let a surgery center or group be affiliated with them. The > surgery center was allowed to join the hospital so the hospital could reep > the revenue generated and process their specimens. Either way, we are all > joined by a common form of employment, and one facility is not better than > another. My field provides jobs and creates revenue just like yours. > Insurance company are going to make changes to try and make revenue during > this change into "OBAMA CARE". Remeber we are not the enemy they are. Who > are they to dictate how my company runs. Insurance companies have to much > power and the decisions they force us to make do not always provide the > best patient care. And that is the ultimate goal for any provider, to give > best patient care right? This is just another hurdle we all must jump > through in these comming changes. I vote we stick together and try our > best to protect all our jobs. Wasnt that long ago that each of us we > trying to get pay increases and bring the importance of our jobs to the > fore front of pathology. The financial squeeze of the helath care system > is going to be felt by all. Histology, pathology, radiology, cytology, we > all must do our best to role with the punches and ensure quality care and > our incomes, as well as our field, reguardless of location. > > Nicole Tatum, HT ASCP > > > > > >? Thank you for that. How are things at Hartford Hospital? One of my >> favorite >> places, rotated there many years ago. Very impressive facility! Is Dr. >> Ricci still there? >> On Mon, Apr 9, 2012 at 4:43 PM, Richard Cartun >> wrote: >> >>> This was released today. >>> >>> Richard >>> >>> Statline Special Alert: >>> New Evidence Links Self-Referral Labs to Increased Utilization, Lower >>> Cancer Detection Rates >>> Study co-funded by CAP Appears in April 2012 Issue of Health Affairs >>> April 9?Self-referring urologists billed Medicare for nearly 75% more >>> anatomic pathology (AP) specimens compared to non self-referring >>> physicians, according to a study published today in a leading health >>> care policy journal. Furthermore, the study found no increase in cancer >>> detection for the patients of self-referring physicians-in fact, the >>> detection rate was 14% lower than that of non self-referring >>> physicians. >>> >>> These findings, from an independent study co-funded by the CAP, provide >>> the first clear evidence that self-referral of anatomic pathology >>> services leads to increased utilization, higher Medicare spending, and >>> lower rates of cancer detection. The study, led by renowned Georgetown >>> University health care economist Jean Mitchell, PhD, will appear in the >>> April 2012 issue of Health Affairs and is now available on the >>> journal?s website. >>> >>> >>> -------------------------------------------------------------------------------- >>> >>> >>> >>> Daniel Schneider 4/9/2012 4:47 PM >>> >>> This is all about the money. The rest is rationalization. >>> >>> The reason a group of non-pathologist physicians opens an in-house >>> pathology lab and hires an employee pathologist is first and foremost >>> to harvest profit from pathology reimbursement. Be a fly on the wall in >>> the >>> partners' meetings and you would know that's what they are talking >>> about. >>> >>> To suggest otherwise is disingenuous. >>> >>> And the implication that the generalist anatomic pathologist is >>> unqualified >>> to be signing out skins, prostates, GI's and whatever is >>> reprehensible. >>> This is not cardiac bypass surgery, and AP pathologists *are* trained >>> to do >>> all of the above. I eagerly defer to subspecialty expert consultants >>> as >>> needed, but most of the time they're not needed. >>> >>> Hospital labs that see few, if any skins, prostates, GI's, are only in >>> that >>> pickle because of the cherrypicking they've already been subjected to. >>> >>> *"in-office AP labs are an emerging frontier of employment for >>> histologists >>> and pathologists.? In an era of high unemployment, another source of >>> employment for our professions is "a good thing.""* >>> >>> Really? The jobs follow the specimens. Given the same number of >>> specimens, >>> there's the same number of jobs, more or less, just under different >>> circumstances and in different locations? Unless you're suggesting >>> that >>> in-office labs will generate increased specimens, and thus increased >>> jobs >>> though overutilization, i.e. excessive numbers of unnecessary biopsies >>> and >>> abuse of the patient and the taxpayer.? In which case I have to say >>> there's >>> a grain of truth. And the truth hurts.? And it's not " a good thing." >>> >>> None of this should be taken as criticism of histotechs and >>> pathologists >>> who find themselves working in an in-office lab. I know there's bills >>> to >>> pay, families to take care of, and god knows it's hard for a >>> pathologist to >>> find a job these days with the numbers our residency programs keep >>> churning >>> out (but that's another rant...). >>> >>> Dan Schneider, MD >>> Amarillo, TX >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> On Mon, Apr 9, 2012 at 12:52 PM, wrote: >>> >>> > >>> > Histonetters: >>> > >>> > In-office AP labs provide a valuable service to the practices they >>> serve >>> > by facilitating 1) better communication between pathologists and >>> ordering >>> > clinicians, 2) quality metrics that are practice-specific, and 3) >>> high >>> > volume, sub-specialization for both histotechnologists and >>> pathologists. >>> >? In other words, the more of one type of histopathology a lab does >>> (e.g., >>> > skin, prostate, GI), the better it gets.? Most people would not think >>> of >>> > having their cardiac bypass surgery done at a community hospital >>> doing >>> > 50/year; you want to go where more than 500/year are done.? In >>> > histopathology, the kinds of volume you want are in the thousands for >>> each >>> > tissue type.? Many hospital labs do little skin or prostate >>> histology >>> > anymore.? Many sub-specialty in-office AP labs may do thousands of >>> cases of >>> > one tissue type every year. >>> > >>> > Aside from that, in-office AP labs are an emerging frontier of >>> employment >>> > for histologists and pathologists.? In an era of high unemployment, >>> another >>> > source of employment for our professions is "a good thing." >>> > >>> > This requirement by an insurer for accreditation will help to >>> validate >>> > these in-office AP labs' commitment to quality and put them on the >>> level >>> > with their hospital counterparts. >>> > >>> > John D. Cochran, MD, FCAP >>> > >>> > >>> > >>> > >>> > >>> > _______________________________________________ >>> > Histonet mailing list >>> > Histonet@lists.utsouthwestern.edu >>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> -- >> *David Costanzo, MHS, PA (ASCP)* >> Project Manager >> *Blufrog Path Lab Solutions* >> 9401 Wilshire Blvd. Ste 650 >> Beverly Hills, CA 90212 >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Tue Apr 10 10:33:05 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Apr 10 10:33:13 2012 Subject: [Histonet] Sweetheart of a day job - Arizona Message-ID: <1334071985.42359.YahooMailNeo@web39402.mail.mud.yahoo.com> Hi Guys- ? I'm SO excited!? One of the places I temped and trained a bench tech oh-so-many years ago is looking to hire another Histotech.? They're growing leaps and bounds.? The Pathologist is the same I worked with--nice guy, good at his job--and the community is awesome.? ? They're looking for a registered or eligible tech with at least one year for a M-F day shift.?It's a hospital--one of those places you can?stay forever and be happy in your job.?The pay is good, relocation assistance is available.? I'd be delighted to help them find their 'right' fit. ? Call my cell or email--attach your resume if you have one or we can write it together... ? Thank you!? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From blueseptember <@t> mail.com Tue Apr 10 10:39:25 2012 From: blueseptember <@t> mail.com (blueseptember@mail.com) Date: Tue Apr 10 10:40:42 2012 Subject: [Histonet] Lab Assistants Message-ID: <20120410153925.4310@gmx.com> Does anyone know of a regulation (CLIA, CAP, JACHO) for a lab assistants / non certified working in histology (embedding, cutting, staining, coverslipping and changing solutions) ? I know many out there have strong "opinions" about this subject but I am interested in the actual regulations. I am needing this to present to my docs. Thanks! From keeping.janet <@t> gmail.com Tue Apr 10 10:55:32 2012 From: keeping.janet <@t> gmail.com (Janet Keeping) Date: Tue Apr 10 10:55:39 2012 Subject: [Histonet] Elastic Stain Message-ID: Are the slides differentiated individually using mcroscopic checks? From nicole <@t> dlcjax.com Tue Apr 10 10:55:59 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Tue Apr 10 10:56:12 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: <344256852160910332@unknownmsgid> References: <344256852160910332@unknownmsgid> Message-ID: <1438.208.62.167.196.1334073359.squirrel@webmail.realpages.com> Rude is when you attack someone who is your equal. Yes, your right im a schmuck because I work in private practice. I didnt know that having my education, and completing my internship, and having 12yrs in the field made me a lesser histologist because I work in private practice. Seriouly get a grip. The conflict lies in you, if you cant see that we all are working to support our families. I really dont care where my fellow Histologist work, because I am happy they have a job and our professional is able to grow and that there are other opportunities for Histologist outside of hospitals. These in-house lab have created all new opportunities for Histologist and I back them 100%. Great thing about being an American, is I dont have to agree with you. This field has supported my family and allowed me to live comfortably, I will defend it for myself and others who will be entering the work force. I can only hope they have me for a mentor. I choose to promote my field and work with my collegues to ensure the survival of all of our jobs. Nicole Tatum HT ASCP You're just plain rude. Whenever someone is wrong, it is easy to > criticize others. Takes the focus off you. > > Unlike you, I will not post my personal rude comments on the entire > list serv. > > You are right, I shouldn't argue with a lesser educated schmuck either. > > Sent from my Windows Phone > From: Nicole Tatum > Sent: 4/10/2012 8:18 AM > To: Davide Costanzo; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation > Really, An undertaker. Yea, theres definately a conflict here, you. No > since in wasting my time. > > > Nicole > > > > > > > Start with reading Dr. Schneider's post. Then read Richard Cartun's >> post. Those should deal will what you are talking about very well. >> >> These in-office labs should not exist, for the very same reason the >> undertaker is no longer the ambulance driver. There is a very real, and >> significant conflict of interest. >> >> Sent from my Windows Phone >> From: Nicole Tatum >> Sent: 4/10/2012 6:45 AM >> To: Davide Costanzo; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation >> Money is at the root of all finicial decisions, in-house labs and >> hospitals. There are many over utilization of resources within the >> health >> care field. Many gallbladder surgerious are performed unneccesarly by >> general surgeous who's practice are within hospitals walls. >> Tonsilectomy. >> etc. How are those specimens not self reffered to the hospitals AP lab. >> David you made the comment about specialities staying with there >> specialty >> and not branching out. A dermatopathologist specializes in derm >> specimens >> so why is it so far fetched that he would read derm specimens from all >> sources, hospitals or in-house labs. My in-house lab has a higher turn >> around rate, lower overhead, and cuts courier fees out. We also do a >> service to our patients by allowing them one stop shopping. We can >> service >> all there needs and they do not have to have multiple appointments at >> different facilities. This cuts down on their copay and billing from >> multiple doctors. Also, it would cost more for a person to have Mohs >> surgery in a hospital setting. As we all know cost are higher at a >> hospital because they have higher overhead. The hospital is self >> reffering >> when they let a surgery center or group be affiliated with them. The >> surgery center was allowed to join the hospital so the hospital could >> reep >> the revenue generated and process their specimens. Either way, we are >> all >> joined by a common form of employment, and one facility is not better >> than >> another. My field provides jobs and creates revenue just like yours. >> Insurance company are going to make changes to try and make revenue >> during >> this change into "OBAMA CARE". Remeber we are not the enemy they are. >> Who >> are they to dictate how my company runs. Insurance companies have to >> much >> power and the decisions they force us to make do not always provide the >> best patient care. And that is the ultimate goal for any provider, to >> give >> best patient care right? This is just another hurdle we all must jump >> through in these comming changes. I vote we stick together and try our >> best to protect all our jobs. Wasnt that long ago that each of us we >> trying to get pay increases and bring the importance of our jobs to the >> fore front of pathology. The financial squeeze of the helath care system >> is going to be felt by all. Histology, pathology, radiology, cytology, >> we >> all must do our best to role with the punches and ensure quality care >> and >> our incomes, as well as our field, reguardless of location. >> >> Nicole Tatum, HT ASCP >> >> >> >> >> >> Thank you for that. How are things at Hartford Hospital? One of my >>> favorite >>> places, rotated there many years ago. Very impressive facility! Is Dr. >>> Ricci still there? >>> On Mon, Apr 9, 2012 at 4:43 PM, Richard Cartun >>> wrote: >>> >>>> This was released today. >>>> >>>> Richard >>>> >>>> Statline Special Alert: >>>> New Evidence Links Self-Referral Labs to Increased Utilization, Lower >>>> Cancer Detection Rates >>>> Study co-funded by CAP Appears in April 2012 Issue of Health Affairs >>>> April 9?Self-referring urologists billed Medicare for nearly 75% more >>>> anatomic pathology (AP) specimens compared to non self-referring >>>> physicians, according to a study published today in a leading health >>>> care policy journal. Furthermore, the study found no increase in >>>> cancer >>>> detection for the patients of self-referring physicians-in fact, the >>>> detection rate was 14% lower than that of non self-referring >>>> physicians. >>>> >>>> These findings, from an independent study co-funded by the CAP, >>>> provide >>>> the first clear evidence that self-referral of anatomic pathology >>>> services leads to increased utilization, higher Medicare spending, and >>>> lower rates of cancer detection. The study, led by renowned Georgetown >>>> University health care economist Jean Mitchell, PhD, will appear in >>>> the >>>> April 2012 issue of Health Affairs and is now available on the >>>> journal?s website. >>>> >>>> >>>> -------------------------------------------------------------------------------- >>>> >>>> >>>> >>> Daniel Schneider 4/9/2012 4:47 PM >>> >>>> This is all about the money. The rest is rationalization. >>>> >>>> The reason a group of non-pathologist physicians opens an in-house >>>> pathology lab and hires an employee pathologist is first and foremost >>>> to harvest profit from pathology reimbursement. Be a fly on the wall >>>> in >>>> the >>>> partners' meetings and you would know that's what they are talking >>>> about. >>>> >>>> To suggest otherwise is disingenuous. >>>> >>>> And the implication that the generalist anatomic pathologist is >>>> unqualified >>>> to be signing out skins, prostates, GI's and whatever is >>>> reprehensible. >>>> This is not cardiac bypass surgery, and AP pathologists *are* trained >>>> to do >>>> all of the above. I eagerly defer to subspecialty expert consultants >>>> as >>>> needed, but most of the time they're not needed. >>>> >>>> Hospital labs that see few, if any skins, prostates, GI's, are only in >>>> that >>>> pickle because of the cherrypicking they've already been subjected to. >>>> >>>> *"in-office AP labs are an emerging frontier of employment for >>>> histologists >>>> and pathologists. In an era of high unemployment, another source of >>>> employment for our professions is "a good thing.""* >>>> >>>> Really? The jobs follow the specimens. Given the same number of >>>> specimens, >>>> there's the same number of jobs, more or less, just under different >>>> circumstances and in different locations Unless you're suggesting >>>> that >>>> in-office labs will generate increased specimens, and thus increased >>>> jobs >>>> though overutilization, i.e. excessive numbers of unnecessary biopsies >>>> and >>>> abuse of the patient and the taxpayer. In which case I have to say >>>> there's >>>> a grain of truth. And the truth hurts. And it's not " a good thing." >>>> >>>> None of this should be taken as criticism of histotechs and >>>> pathologists >>>> who find themselves working in an in-office lab. I know there's bills >>>> to >>>> pay, families to take care of, and god knows it's hard for a >>>> pathologist to >>>> find a job these days with the numbers our residency programs keep >>>> churning >>>> out (but that's another rant...). >>>> >>>> Dan Schneider, MD >>>> Amarillo, TX >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> On Mon, Apr 9, 2012 at 12:52 PM, wrote: >>>> >>>> > >>>> > Histonetters: >>>> > >>>> > In-office AP labs provide a valuable service to the practices they >>>> serve >>>> > by facilitating 1) better communication between pathologists and >>>> ordering >>>> > clinicians, 2) quality metrics that are practice-specific, and 3) >>>> high >>>> > volume, sub-specialization for both histotechnologists and >>>> pathologists. >>>> > In other words, the more of one type of histopathology a lab does >>>> (e.g., >>>> > skin, prostate, GI), the better it gets. Most people would not >>>> think >>>> of >>>> > having their cardiac bypass surgery done at a community hospital >>>> doing >>>> > 50/year; you want to go where more than 500/year are done. In >>>> > histopathology, the kinds of volume you want are in the thousands >>>> for >>>> each >>>> > tissue type. Many hospital labs do little skin or prostate >>>> histology >>>> > anymore. Many sub-specialty in-office AP labs may do thousands of >>>> cases of >>>> > one tissue type every year. >>>> > >>>> > Aside from that, in-office AP labs are an emerging frontier of >>>> employment >>>> > for histologists and pathologists. In an era of high unemployment, >>>> another >>>> > source of employment for our professions is "a good thing." >>>> > >>>> > This requirement by an insurer for accreditation will help to >>>> validate >>>> > these in-office AP labs' commitment to quality and put them on the >>>> level >>>> > with their hospital counterparts. >>>> > >>>> > John D. Cochran, MD, FCAP >>>> > >>>> > >>>> > >>>> > >>>> > >>>> > _______________________________________________ >>>> > Histonet mailing list >>>> > Histonet@lists.utsouthwestern.edu >>>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> > >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>> >>> >>> >>> -- >>> *David Costanzo, MHS, PA (ASCP)* >>> Project Manager >>> *Blufrog Path Lab Solutions* >>> 9401 Wilshire Blvd. Ste 650 >>> Beverly Hills, CA 90212 >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> > From relia1 <@t> earthlink.net Tue Apr 10 10:57:46 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Apr 10 10:57:52 2012 Subject: [Histonet] Hot Histology Job Alert from RELIA Solutions. Histology Tech needed - Days Full time in Kingman, AZ Message-ID: Hi Histonetters. I hope everyone is having a great day. I am excited to tell you about an opportunity that I have with a client in Kingman, AZ. This is a fulltime permanent day shift position and my client offers a competitive salary, nice benefits and a great group of people to work with. They need someone who is ASCP certified or eligible and has at least 1 year of histology experience preferably in a hospital environment. I have heard great things about this facility and would love to introduce you to them. If you would like more information please contact me toll free at 866-607-3542 or by email at relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From HParker <@t> Skaggs.Net Tue Apr 10 11:00:47 2012 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Tue Apr 10 11:00:55 2012 Subject: [Histonet] Slippery Floors due to paraffin In-Reply-To: References: Message-ID: <930EB2E8DF68C544873EDD2A3D5F506005BD3A4530@email1.skaggs.net> I was also taught years ago to make sure housekeeping does not put wax on your path lab floors. Helayne Parker, H.T. (ASCP) Pathology Section Head Skaggs Regional Medical Center The Best Place to Get Better P.O. Box 650, Branson Missouri 65615 Direct: 417-335-7254 Fax: 417-335-7127 E-Mail: hparker@skaggs.net Web: www.skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. From pathlocums <@t> gmail.com Tue Apr 10 11:13:16 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Tue Apr 10 11:13:19 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation Message-ID: <-4576423096236240132@unknownmsgid> Very classy argument. Thank you for your eloquence. Sent from my Windows Phone From: Nicole Tatum Sent: 4/10/2012 8:18 AM To: Davide Costanzo; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation Really, An undertaker. Yea, theres definately a conflict here, you. No since in wasting my time. Nicole Start with reading Dr. Schneider's post. Then read Richard Cartun's > post. Those should deal will what you are talking about very well. > > These in-office labs should not exist, for the very same reason the > undertaker is no longer the ambulance driver. There is a very real, and > significant conflict of interest. > > Sent from my Windows Phone > From: Nicole Tatum > Sent: 4/10/2012 6:45 AM > To: Davide Costanzo; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation > Money is at the root of all finicial decisions, in-house labs and > hospitals. There are many over utilization of resources within the health > care field. Many gallbladder surgerious are performed unneccesarly by > general surgeous who's practice are within hospitals walls. Tonsilectomy. > etc. How are those specimens not self reffered to the hospitals AP lab. > David you made the comment about specialities staying with there specialty > and not branching out. A dermatopathologist specializes in derm specimens > so why is it so far fetched that he would read derm specimens from all > sources, hospitals or in-house labs. My in-house lab has a higher turn > around rate, lower overhead, and cuts courier fees out. We also do a > service to our patients by allowing them one stop shopping. We can service > all there needs and they do not have to have multiple appointments at > different facilities. This cuts down on their copay and billing from > multiple doctors. Also, it would cost more for a person to have Mohs > surgery in a hospital setting. As we all know cost are higher at a > hospital because they have higher overhead. The hospital is self reffering > when they let a surgery center or group be affiliated with them. The > surgery center was allowed to join the hospital so the hospital could reep > the revenue generated and process their specimens. Either way, we are all > joined by a common form of employment, and one facility is not better than > another. My field provides jobs and creates revenue just like yours. > Insurance company are going to make changes to try and make revenue during > this change into "OBAMA CARE". Remeber we are not the enemy they are. Who > are they to dictate how my company runs. Insurance companies have to much > power and the decisions they force us to make do not always provide the > best patient care. And that is the ultimate goal for any provider, to give > best patient care right? This is just another hurdle we all must jump > through in these comming changes. I vote we stick together and try our > best to protect all our jobs. Wasnt that long ago that each of us we > trying to get pay increases and bring the importance of our jobs to the > fore front of pathology. The financial squeeze of the helath care system > is going to be felt by all. Histology, pathology, radiology, cytology, we > all must do our best to role with the punches and ensure quality care and > our incomes, as well as our field, reguardless of location. > > Nicole Tatum, HT ASCP > > > > > > Thank you for that. How are things at Hartford Hospital? One of my >> favorite >> places, rotated there many years ago. Very impressive facility! Is Dr. >> Ricci still there? >> On Mon, Apr 9, 2012 at 4:43 PM, Richard Cartun >> wrote: >> >>> This was released today. >>> >>> Richard >>> >>> Statline Special Alert: >>> New Evidence Links Self-Referral Labs to Increased Utilization, Lower >>> Cancer Detection Rates >>> Study co-funded by CAP Appears in April 2012 Issue of Health Affairs >>> April 9?Self-referring urologists billed Medicare for nearly 75% more >>> anatomic pathology (AP) specimens compared to non self-referring >>> physicians, according to a study published today in a leading health >>> care policy journal. Furthermore, the study found no increase in cancer >>> detection for the patients of self-referring physicians-in fact, the >>> detection rate was 14% lower than that of non self-referring >>> physicians. >>> >>> These findings, from an independent study co-funded by the CAP, provide >>> the first clear evidence that self-referral of anatomic pathology >>> services leads to increased utilization, higher Medicare spending, and >>> lower rates of cancer detection. The study, led by renowned Georgetown >>> University health care economist Jean Mitchell, PhD, will appear in the >>> April 2012 issue of Health Affairs and is now available on the >>> journal?s website. >>> >>> >>> -------------------------------------------------------------------------------- >>> >>> >>> >>> Daniel Schneider 4/9/2012 4:47 PM >>> >>> This is all about the money. The rest is rationalization. >>> >>> The reason a group of non-pathologist physicians opens an in-house >>> pathology lab and hires an employee pathologist is first and foremost >>> to harvest profit from pathology reimbursement. Be a fly on the wall in >>> the >>> partners' meetings and you would know that's what they are talking >>> about. >>> >>> To suggest otherwise is disingenuous. >>> >>> And the implication that the generalist anatomic pathologist is >>> unqualified >>> to be signing out skins, prostates, GI's and whatever is >>> reprehensible. >>> This is not cardiac bypass surgery, and AP pathologists *are* trained >>> to do >>> all of the above. I eagerly defer to subspecialty expert consultants >>> as >>> needed, but most of the time they're not needed. >>> >>> Hospital labs that see few, if any skins, prostates, GI's, are only in >>> that >>> pickle because of the cherrypicking they've already been subjected to. >>> >>> *"in-office AP labs are an emerging frontier of employment for >>> histologists >>> and pathologists. In an era of high unemployment, another source of >>> employment for our professions is "a good thing.""* >>> >>> Really? The jobs follow the specimens. Given the same number of >>> specimens, >>> there's the same number of jobs, more or less, just under different >>> circumstances and in different locations Unless you're suggesting >>> that >>> in-office labs will generate increased specimens, and thus increased >>> jobs >>> though overutilization, i.e. excessive numbers of unnecessary biopsies >>> and >>> abuse of the patient and the taxpayer. In which case I have to say >>> there's >>> a grain of truth. And the truth hurts. And it's not " a good thing." >>> >>> None of this should be taken as criticism of histotechs and >>> pathologists >>> who find themselves working in an in-office lab. I know there's bills >>> to >>> pay, families to take care of, and god knows it's hard for a >>> pathologist to >>> find a job these days with the numbers our residency programs keep >>> churning >>> out (but that's another rant...). >>> >>> Dan Schneider, MD >>> Amarillo, TX >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> On Mon, Apr 9, 2012 at 12:52 PM, wrote: >>> >>> > >>> > Histonetters: >>> > >>> > In-office AP labs provide a valuable service to the practices they >>> serve >>> > by facilitating 1) better communication between pathologists and >>> ordering >>> > clinicians, 2) quality metrics that are practice-specific, and 3) >>> high >>> > volume, sub-specialization for both histotechnologists and >>> pathologists. >>> > In other words, the more of one type of histopathology a lab does >>> (e.g., >>> > skin, prostate, GI), the better it gets. Most people would not think >>> of >>> > having their cardiac bypass surgery done at a community hospital >>> doing >>> > 50/year; you want to go where more than 500/year are done. In >>> > histopathology, the kinds of volume you want are in the thousands for >>> each >>> > tissue type. Many hospital labs do little skin or prostate >>> histology >>> > anymore. Many sub-specialty in-office AP labs may do thousands of >>> cases of >>> > one tissue type every year. >>> > >>> > Aside from that, in-office AP labs are an emerging frontier of >>> employment >>> > for histologists and pathologists. In an era of high unemployment, >>> another >>> > source of employment for our professions is "a good thing." >>> > >>> > This requirement by an insurer for accreditation will help to >>> validate >>> > these in-office AP labs' commitment to quality and put them on the >>> level >>> > with their hospital counterparts. >>> > >>> > John D. Cochran, MD, FCAP >>> > >>> > >>> > >>> > >>> > >>> > _______________________________________________ >>> > Histonet mailing list >>> > Histonet@lists.utsouthwestern.edu >>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> -- >> *David Costanzo, MHS, PA (ASCP)* >> Project Manager >> *Blufrog Path Lab Solutions* >> 9401 Wilshire Blvd. Ste 650 >> Beverly Hills, CA 90212 >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From jqb7 <@t> cdc.gov Tue Apr 10 11:14:01 2012 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Apr 10 11:14:06 2012 Subject: [Histonet] RE: Microwave processors In-Reply-To: <24B7B291CC88D04AB663958E77A1F59D02E3D6@ex09.net.ucsf.edu> References: <24B7B291CC88D04AB663958E77A1F59D02E3D6@ex09.net.ucsf.edu> Message-ID: Erin, We have Sakura's Xpress and skins have always turned out just fine for us. It is very easy to use and maintain. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 Jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Tuesday, April 10, 2012 10:38 AM To: histonet Subject: [Histonet] Microwave processors Hi histonetters! Our pathologists want to turn around skin biopsies same day and are again looking at microwave processors. Due to a bad past experience, I'm not enthused but perhaps there is someone out there who loves their microwave processor? Even on derm? Or has anyone worked out a good rapid derm processing protocol on a conventional processor? Thank you so much! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any actin in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt <@t> uphs.upenn.edu Tue Apr 10 11:36:00 2012 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Tue Apr 10 11:35:47 2012 Subject: [Histonet] RE: Microwave processors In-Reply-To: References: <24B7B291CC88D04AB663958E77A1F59D02E3D6@ex09.net.ucsf.edu> Message-ID: We cannot say the same, we had issues with shaves appearing "cooked". We had specialists out several times and after many suggestions, nothing resolved the issue. It doesn't happen consistently but it definitely happens and we even attempted to track by tech or shifts or when the solutions were changed and no patterns could be found after several years. Sakura has a new vendor for reagents now and they are going to come run some test slides but the VIP quality for derm keeps the pathologists much happier based on our experience. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, April 10, 2012 12:14 PM To: Martin, Erin; histonet Subject: [Histonet] RE: Microwave processors Erin, We have Sakura's Xpress and skins have always turned out just fine for us. It is very easy to use and maintain. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 Jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Tuesday, April 10, 2012 10:38 AM To: histonet Subject: [Histonet] Microwave processors Hi histonetters! Our pathologists want to turn around skin biopsies same day and are again looking at microwave processors. Due to a bad past experience, I'm not enthused but perhaps there is someone out there who loves their microwave processor? Even on derm? Or has anyone worked out a good rapid derm processing protocol on a conventional processor? Thank you so much! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any actin in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Caroline.Pratt <@t> uphs.upenn.edu Tue Apr 10 11:37:35 2012 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Tue Apr 10 11:37:24 2012 Subject: [Histonet] Microwave processors In-Reply-To: <24B7B291CC88D04AB663958E77A1F59D02E3D6@ex09.net.ucsf.edu> References: <24B7B291CC88D04AB663958E77A1F59D02E3D6@ex09.net.ucsf.edu> Message-ID: I believe there was a conference this month in your area and they were introducing a new to the market rapid traditional constant feed processor. Let me see if I can track down the info. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Tuesday, April 10, 2012 10:38 AM To: histonet Subject: [Histonet] Microwave processors Hi histonetters! Our pathologists want to turn around skin biopsies same day and are again looking at microwave processors. Due to a bad past experience, I'm not enthused but perhaps there is someone out there who loves their microwave processor? Even on derm? Or has anyone worked out a good rapid derm processing protocol on a conventional processor? Thank you so much! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any actin in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From lhadley <@t> iupui.edu Tue Apr 10 11:33:22 2012 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Tue Apr 10 11:39:43 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: <-4576423096236240132@unknownmsgid> References: <-4576423096236240132@unknownmsgid> Message-ID: <8638FBDA16B0584D82AA21CD236FF97F2DD90177@IU-MSSG-MBX110.ads.iu.edu> Hey David I think your list of people never wanting to work with you just got longer. Lee Ann -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo Sent: Tuesday, April 10, 2012 12:13 PM To: Nicole Tatum; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation Very classy argument. Thank you for your eloquence. Sent from my Windows Phone From: Nicole Tatum Sent: 4/10/2012 8:18 AM To: Davide Costanzo; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation Really, An undertaker. Yea, theres definately a conflict here, you. No since in wasting my time. Nicole Start with reading Dr. Schneider's post. Then read Richard Cartun's > post. Those should deal will what you are talking about very well. > > These in-office labs should not exist, for the very same reason the > undertaker is no longer the ambulance driver. There is a very real, and > significant conflict of interest. > > Sent from my Windows Phone > From: Nicole Tatum > Sent: 4/10/2012 6:45 AM > To: Davide Costanzo; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation > Money is at the root of all finicial decisions, in-house labs and > hospitals. There are many over utilization of resources within the health > care field. Many gallbladder surgerious are performed unneccesarly by > general surgeous who's practice are within hospitals walls. Tonsilectomy. > etc. How are those specimens not self reffered to the hospitals AP lab. > David you made the comment about specialities staying with there specialty > and not branching out. A dermatopathologist specializes in derm specimens > so why is it so far fetched that he would read derm specimens from all > sources, hospitals or in-house labs. My in-house lab has a higher turn > around rate, lower overhead, and cuts courier fees out. We also do a > service to our patients by allowing them one stop shopping. We can service > all there needs and they do not have to have multiple appointments at > different facilities. This cuts down on their copay and billing from > multiple doctors. Also, it would cost more for a person to have Mohs > surgery in a hospital setting. As we all know cost are higher at a > hospital because they have higher overhead. The hospital is self reffering > when they let a surgery center or group be affiliated with them. The > surgery center was allowed to join the hospital so the hospital could reep > the revenue generated and process their specimens. Either way, we are all > joined by a common form of employment, and one facility is not better than > another. My field provides jobs and creates revenue just like yours. > Insurance company are going to make changes to try and make revenue during > this change into "OBAMA CARE". Remeber we are not the enemy they are. Who > are they to dictate how my company runs. Insurance companies have to much > power and the decisions they force us to make do not always provide the > best patient care. And that is the ultimate goal for any provider, to give > best patient care right? This is just another hurdle we all must jump > through in these comming changes. I vote we stick together and try our > best to protect all our jobs. Wasnt that long ago that each of us we > trying to get pay increases and bring the importance of our jobs to the > fore front of pathology. The financial squeeze of the helath care system > is going to be felt by all. Histology, pathology, radiology, cytology, we > all must do our best to role with the punches and ensure quality care and > our incomes, as well as our field, reguardless of location. > > Nicole Tatum, HT ASCP > > > > > > Thank you for that. How are things at Hartford Hospital? One of my >> favorite >> places, rotated there many years ago. Very impressive facility! Is Dr. >> Ricci still there? >> On Mon, Apr 9, 2012 at 4:43 PM, Richard Cartun >> wrote: >> >>> This was released today. >>> >>> Richard >>> >>> Statline Special Alert: >>> New Evidence Links Self-Referral Labs to Increased Utilization, Lower >>> Cancer Detection Rates >>> Study co-funded by CAP Appears in April 2012 Issue of Health Affairs >>> April 9-Self-referring urologists billed Medicare for nearly 75% more >>> anatomic pathology (AP) specimens compared to non self-referring >>> physicians, according to a study published today in a leading health >>> care policy journal. Furthermore, the study found no increase in cancer >>> detection for the patients of self-referring physicians-in fact, the >>> detection rate was 14% lower than that of non self-referring >>> physicians. >>> >>> These findings, from an independent study co-funded by the CAP, provide >>> the first clear evidence that self-referral of anatomic pathology >>> services leads to increased utilization, higher Medicare spending, and >>> lower rates of cancer detection. The study, led by renowned Georgetown >>> University health care economist Jean Mitchell, PhD, will appear in the >>> April 2012 issue of Health Affairs and is now available on the >>> journal's website. >>> >>> >>> -------------------------------------------------------------------------------- >>> >>> >>> >>> Daniel Schneider 4/9/2012 4:47 PM >>> >>> This is all about the money. The rest is rationalization. >>> >>> The reason a group of non-pathologist physicians opens an in-house >>> pathology lab and hires an employee pathologist is first and foremost >>> to harvest profit from pathology reimbursement. Be a fly on the wall in >>> the >>> partners' meetings and you would know that's what they are talking >>> about. >>> >>> To suggest otherwise is disingenuous. >>> >>> And the implication that the generalist anatomic pathologist is >>> unqualified >>> to be signing out skins, prostates, GI's and whatever is >>> reprehensible. >>> This is not cardiac bypass surgery, and AP pathologists *are* trained >>> to do >>> all of the above. I eagerly defer to subspecialty expert consultants >>> as >>> needed, but most of the time they're not needed. >>> >>> Hospital labs that see few, if any skins, prostates, GI's, are only in >>> that >>> pickle because of the cherrypicking they've already been subjected to. >>> >>> *"in-office AP labs are an emerging frontier of employment for >>> histologists >>> and pathologists. In an era of high unemployment, another source of >>> employment for our professions is "a good thing.""* >>> >>> Really? The jobs follow the specimens. Given the same number of >>> specimens, >>> there's the same number of jobs, more or less, just under different >>> circumstances and in different locations Unless you're suggesting >>> that >>> in-office labs will generate increased specimens, and thus increased >>> jobs >>> though overutilization, i.e. excessive numbers of unnecessary biopsies >>> and >>> abuse of the patient and the taxpayer. In which case I have to say >>> there's >>> a grain of truth. And the truth hurts. And it's not " a good thing." >>> >>> None of this should be taken as criticism of histotechs and >>> pathologists >>> who find themselves working in an in-office lab. I know there's bills >>> to >>> pay, families to take care of, and god knows it's hard for a >>> pathologist to >>> find a job these days with the numbers our residency programs keep >>> churning >>> out (but that's another rant...). >>> >>> Dan Schneider, MD >>> Amarillo, TX >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> On Mon, Apr 9, 2012 at 12:52 PM, wrote: >>> >>> > >>> > Histonetters: >>> > >>> > In-office AP labs provide a valuable service to the practices they >>> serve >>> > by facilitating 1) better communication between pathologists and >>> ordering >>> > clinicians, 2) quality metrics that are practice-specific, and 3) >>> high >>> > volume, sub-specialization for both histotechnologists and >>> pathologists. >>> > In other words, the more of one type of histopathology a lab does >>> (e.g., >>> > skin, prostate, GI), the better it gets. Most people would not think >>> of >>> > having their cardiac bypass surgery done at a community hospital >>> doing >>> > 50/year; you want to go where more than 500/year are done. In >>> > histopathology, the kinds of volume you want are in the thousands for >>> each >>> > tissue type. Many hospital labs do little skin or prostate >>> histology >>> > anymore. Many sub-specialty in-office AP labs may do thousands of >>> cases of >>> > one tissue type every year. >>> > >>> > Aside from that, in-office AP labs are an emerging frontier of >>> employment >>> > for histologists and pathologists. In an era of high unemployment, >>> another >>> > source of employment for our professions is "a good thing." >>> > >>> > This requirement by an insurer for accreditation will help to >>> validate >>> > these in-office AP labs' commitment to quality and put them on the >>> level >>> > with their hospital counterparts. >>> > >>> > John D. Cochran, MD, FCAP >>> > >>> > >>> > >>> > >>> > >>> > _______________________________________________ >>> > Histonet mailing list >>> > Histonet@lists.utsouthwestern.edu >>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> -- >> *David Costanzo, MHS, PA (ASCP)* >> Project Manager >> *Blufrog Path Lab Solutions* >> 9401 Wilshire Blvd. Ste 650 >> Beverly Hills, CA 90212 >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Tue Apr 10 11:43:26 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Apr 10 11:43:34 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: <-4576423096236240132@unknownmsgid> References: <-4576423096236240132@unknownmsgid> Message-ID: <1334076206.51518.YahooMailNeo@web112302.mail.gq1.yahoo.com> Davide, ???????????? You are seriously offending a mass of people who work very hard in private labs. And you do it again with this comeback. You have NOT been eloquent yourself so get off the high horse. ? We need to end this topic. Apparently we are devided amongst ourselves into private labs and hospital labs. Thats too bad as I have worked in both and they both provide a needed service. ? And Davide, Trust me, I know Nicole personally and she is the "definition" of Class. ? Have a good week all! ? Kim D ________________________________ From: Davide Costanzo To: Nicole Tatum ; "histonet@lists.utsouthwestern.edu" Sent: Tuesday, April 10, 2012 12:13 PM Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation Very classy argument. Thank you for your eloquence. Sent from my Windows Phone From: Nicole Tatum Sent: 4/10/2012 8:18 AM To: Davide Costanzo; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation Really, An undertaker. Yea, theres definately a conflict here, you. No since in wasting my time. Nicole Start with reading Dr. Schneider's post. Then read Richard Cartun's > post. Those should deal will what you are talking about very well. > > These in-office labs should not exist, for the very same reason the > undertaker is no longer the ambulance driver. There is a very real, and > significant conflict of interest. > > Sent from my Windows Phone > From: Nicole Tatum > Sent: 4/10/2012 6:45 AM > To: Davide Costanzo; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation > Money is at the root of all finicial decisions, in-house labs and > hospitals. There are many over utilization of resources within the health > care field. Many gallbladder surgerious are performed unneccesarly by > general surgeous who's practice are within hospitals walls. Tonsilectomy. > etc. How are those specimens not self reffered to the hospitals AP lab. > David you made the comment about specialities staying with there specialty > and not branching out. A dermatopathologist specializes in derm specimens > so why is it so far fetched that he would read derm specimens from all > sources, hospitals or in-house labs. My in-house lab has a higher turn > around rate, lower overhead, and cuts courier fees out. We also do a > service to our patients by allowing them one stop shopping. We can service > all there needs and they do not have to have multiple appointments at > different facilities. This cuts down on their copay and billing from > multiple doctors. Also, it would cost more for a person to have Mohs > surgery in a hospital setting. As we all know cost are higher at a > hospital because they have higher overhead. The hospital is self reffering > when they let a surgery center or group be affiliated with them. The > surgery center was allowed to join the hospital so the hospital could reep > the revenue generated and process their specimens. Either way, we are all > joined by a common form of employment, and one facility is not better than > another. My field provides jobs and creates revenue just like yours. > Insurance company are going to make changes to try and make revenue during > this change into "OBAMA CARE". Remeber we are not the enemy they are. Who > are they to dictate how my company runs. Insurance companies have to much > power and the decisions they force us to make do not always provide the > best patient care. And that is the ultimate goal for any provider, to give > best patient care right? This is just another hurdle we all must jump > through in these comming changes. I vote we stick together and try our > best to protect all our jobs. Wasnt that long ago that each of us we > trying to get pay increases and bring the importance of our jobs to the > fore front of pathology. The financial squeeze of the helath care system > is going to be felt by all. Histology, pathology, radiology, cytology, we > all must do our best to role with the punches and ensure quality care and > our incomes, as well as our field, reguardless of location. > > Nicole Tatum, HT ASCP > > > > > >? Thank you for that. How are things at Hartford Hospital? One of my >> favorite >> places, rotated there many years ago. Very impressive facility! Is Dr. >> Ricci still there? >> On Mon, Apr 9, 2012 at 4:43 PM, Richard Cartun >> wrote: >> >>> This was released today. >>> >>> Richard >>> >>> Statline Special Alert: >>> New Evidence Links Self-Referral Labs to Increased Utilization, Lower >>> Cancer Detection Rates >>> Study co-funded by CAP Appears in April 2012 Issue of Health Affairs >>> April 9?Self-referring urologists billed Medicare for nearly 75% more >>> anatomic pathology (AP) specimens compared to non self-referring >>> physicians, according to a study published today in a leading health >>> care policy journal. Furthermore, the study found no increase in cancer >>> detection for the patients of self-referring physicians-in fact, the >>> detection rate was 14% lower than that of non self-referring >>> physicians. >>> >>> These findings, from an independent study co-funded by the CAP, provide >>> the first clear evidence that self-referral of anatomic pathology >>> services leads to increased utilization, higher Medicare spending, and >>> lower rates of cancer detection. The study, led by renowned Georgetown >>> University health care economist Jean Mitchell, PhD, will appear in the >>> April 2012 issue of Health Affairs and is now available on the >>> journal?s website. >>> >>> >>> -------------------------------------------------------------------------------- >>> >>> >>> >>> Daniel Schneider 4/9/2012 4:47 PM >>> >>> This is all about the money. The rest is rationalization. >>> >>> The reason a group of non-pathologist physicians opens an in-house >>> pathology lab and hires an employee pathologist is first and foremost >>> to harvest profit from pathology reimbursement. Be a fly on the wall in >>> the >>> partners' meetings and you would know that's what they are talking >>> about. >>> >>> To suggest otherwise is disingenuous. >>> >>> And the implication that the generalist anatomic pathologist is >>> unqualified >>> to be signing out skins, prostates, GI's and whatever is >>> reprehensible. >>> This is not cardiac bypass surgery, and AP pathologists *are* trained >>> to do >>> all of the above. I eagerly defer to subspecialty expert consultants >>> as >>> needed, but most of the time they're not needed. >>> >>> Hospital labs that see few, if any skins, prostates, GI's, are only in >>> that >>> pickle because of the cherrypicking they've already been subjected to. >>> >>> *"in-office AP labs are an emerging frontier of employment for >>> histologists >>> and pathologists.? In an era of high unemployment, another source of >>> employment for our professions is "a good thing.""* >>> >>> Really? The jobs follow the specimens. Given the same number of >>> specimens, >>> there's the same number of jobs, more or less, just under different >>> circumstances and in different locations? Unless you're suggesting >>> that >>> in-office labs will generate increased specimens, and thus increased >>> jobs >>> though overutilization, i.e. excessive numbers of unnecessary biopsies >>> and >>> abuse of the patient and the taxpayer.? In which case I have to say >>> there's >>> a grain of truth. And the truth hurts.? And it's not " a good thing." >>> >>> None of this should be taken as criticism of histotechs and >>> pathologists >>> who find themselves working in an in-office lab. I know there's bills >>> to >>> pay, families to take care of, and god knows it's hard for a >>> pathologist to >>> find a job these days with the numbers our residency programs keep >>> churning >>> out (but that's another rant...). >>> >>> Dan Schneider, MD >>> Amarillo, TX >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> On Mon, Apr 9, 2012 at 12:52 PM, wrote: >>> >>> > >>> > Histonetters: >>> > >>> > In-office AP labs provide a valuable service to the practices they >>> serve >>> > by facilitating 1) better communication between pathologists and >>> ordering >>> > clinicians, 2) quality metrics that are practice-specific, and 3) >>> high >>> > volume, sub-specialization for both histotechnologists and >>> pathologists. >>> >? In other words, the more of one type of histopathology a lab does >>> (e.g., >>> > skin, prostate, GI), the better it gets.? Most people would not think >>> of >>> > having their cardiac bypass surgery done at a community hospital >>> doing >>> > 50/year; you want to go where more than 500/year are done.? In >>> > histopathology, the kinds of volume you want are in the thousands for >>> each >>> > tissue type.? Many hospital labs do little skin or prostate >>> histology >>> > anymore.? Many sub-specialty in-office AP labs may do thousands of >>> cases of >>> > one tissue type every year. >>> > >>> > Aside from that, in-office AP labs are an emerging frontier of >>> employment >>> > for histologists and pathologists.? In an era of high unemployment, >>> another >>> > source of employment for our professions is "a good thing." >>> > >>> > This requirement by an insurer for accreditation will help to >>> validate >>> > these in-office AP labs' commitment to quality and put them on the >>> level >>> > with their hospital counterparts. >>> > >>> > John D. Cochran, MD, FCAP >>> > >>> > >>> > >>> > >>> > >>> > _______________________________________________ >>> > Histonet mailing list >>> > Histonet@lists.utsouthwestern.edu >>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> -- >> *David Costanzo, MHS, PA (ASCP)* >> Project Manager >> *Blufrog Path Lab Solutions* >> 9401 Wilshire Blvd. Ste 650 >> Beverly Hills, CA 90212 >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt <@t> uphs.upenn.edu Tue Apr 10 12:18:54 2012 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Tue Apr 10 12:18:41 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: <1438.208.62.167.196.1334073359.squirrel@webmail.realpages.com> References: <344256852160910332@unknownmsgid> <1438.208.62.167.196.1334073359.squirrel@webmail.realpages.com> Message-ID: I don't think it was meant as a personal attack, it's a larger conceptual issue on ethics of the business principle behind the model for in-office laboratories and the debate isn't about jobs, it's about the best interest of the patient. I am sure your skill set is exceptional. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Tuesday, April 10, 2012 11:56 AM To: Davide Costanzo; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation Rude is when you attack someone who is your equal. Yes, your right im a schmuck because I work in private practice. I didnt know that having my education, and completing my internship, and having 12yrs in the field made me a lesser histologist because I work in private practice. Seriouly get a grip. The conflict lies in you, if you cant see that we all are working to support our families. I really dont care where my fellow Histologist work, because I am happy they have a job and our professional is able to grow and that there are other opportunities for Histologist outside of hospitals. These in-house lab have created all new opportunities for Histologist and I back them 100%. Great thing about being an American, is I dont have to agree with you. This field has supported my family and allowed me to live comfortably, I will defend it for myself and others who will be entering the work force. I can only hope they have me for a mentor. I choose to promote my field and work with my collegues to ensure the survival of all of our jobs. Nicole Tatum HT ASCP You're just plain rude. Whenever someone is wrong, it is easy to > criticize others. Takes the focus off you. > > Unlike you, I will not post my personal rude comments on the entire > list serv. > > You are right, I shouldn't argue with a lesser educated schmuck either. > > Sent from my Windows Phone > From: Nicole Tatum > Sent: 4/10/2012 8:18 AM > To: Davide Costanzo; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation > Really, An undertaker. Yea, theres definately a conflict here, you. No > since in wasting my time. > > > Nicole > > > > > > > Start with reading Dr. Schneider's post. Then read Richard Cartun's >> post. Those should deal will what you are talking about very well. >> >> These in-office labs should not exist, for the very same reason the >> undertaker is no longer the ambulance driver. There is a very real, and >> significant conflict of interest. >> >> Sent from my Windows Phone >> From: Nicole Tatum >> Sent: 4/10/2012 6:45 AM >> To: Davide Costanzo; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation >> Money is at the root of all finicial decisions, in-house labs and >> hospitals. There are many over utilization of resources within the >> health >> care field. Many gallbladder surgerious are performed unneccesarly by >> general surgeous who's practice are within hospitals walls. >> Tonsilectomy. >> etc. How are those specimens not self reffered to the hospitals AP lab. >> David you made the comment about specialities staying with there >> specialty >> and not branching out. A dermatopathologist specializes in derm >> specimens >> so why is it so far fetched that he would read derm specimens from all >> sources, hospitals or in-house labs. My in-house lab has a higher turn >> around rate, lower overhead, and cuts courier fees out. We also do a >> service to our patients by allowing them one stop shopping. We can >> service >> all there needs and they do not have to have multiple appointments at >> different facilities. This cuts down on their copay and billing from >> multiple doctors. Also, it would cost more for a person to have Mohs >> surgery in a hospital setting. As we all know cost are higher at a >> hospital because they have higher overhead. The hospital is self >> reffering >> when they let a surgery center or group be affiliated with them. The >> surgery center was allowed to join the hospital so the hospital could >> reep >> the revenue generated and process their specimens. Either way, we are >> all >> joined by a common form of employment, and one facility is not better >> than >> another. My field provides jobs and creates revenue just like yours. >> Insurance company are going to make changes to try and make revenue >> during >> this change into "OBAMA CARE". Remeber we are not the enemy they are. >> Who >> are they to dictate how my company runs. Insurance companies have to >> much >> power and the decisions they force us to make do not always provide the >> best patient care. And that is the ultimate goal for any provider, to >> give >> best patient care right? This is just another hurdle we all must jump >> through in these comming changes. I vote we stick together and try our >> best to protect all our jobs. Wasnt that long ago that each of us we >> trying to get pay increases and bring the importance of our jobs to the >> fore front of pathology. The financial squeeze of the helath care system >> is going to be felt by all. Histology, pathology, radiology, cytology, >> we >> all must do our best to role with the punches and ensure quality care >> and >> our incomes, as well as our field, reguardless of location. >> >> Nicole Tatum, HT ASCP >> >> >> >> >> >> Thank you for that. How are things at Hartford Hospital? One of my >>> favorite >>> places, rotated there many years ago. Very impressive facility! Is Dr. >>> Ricci still there? >>> On Mon, Apr 9, 2012 at 4:43 PM, Richard Cartun >>> wrote: >>> >>>> This was released today. >>>> >>>> Richard >>>> >>>> Statline Special Alert: >>>> New Evidence Links Self-Referral Labs to Increased Utilization, Lower >>>> Cancer Detection Rates >>>> Study co-funded by CAP Appears in April 2012 Issue of Health Affairs >>>> April 9-Self-referring urologists billed Medicare for nearly 75% more >>>> anatomic pathology (AP) specimens compared to non self-referring >>>> physicians, according to a study published today in a leading health >>>> care policy journal. Furthermore, the study found no increase in >>>> cancer >>>> detection for the patients of self-referring physicians-in fact, the >>>> detection rate was 14% lower than that of non self-referring >>>> physicians. >>>> >>>> These findings, from an independent study co-funded by the CAP, >>>> provide >>>> the first clear evidence that self-referral of anatomic pathology >>>> services leads to increased utilization, higher Medicare spending, and >>>> lower rates of cancer detection. The study, led by renowned Georgetown >>>> University health care economist Jean Mitchell, PhD, will appear in >>>> the >>>> April 2012 issue of Health Affairs and is now available on the >>>> journal's website. >>>> >>>> >>>> ------------------------------------------------------------------------ -------- >>>> >>>> >>>> >>> Daniel Schneider 4/9/2012 4:47 PM >>> >>>> This is all about the money. The rest is rationalization. >>>> >>>> The reason a group of non-pathologist physicians opens an in-house >>>> pathology lab and hires an employee pathologist is first and foremost >>>> to harvest profit from pathology reimbursement. Be a fly on the wall >>>> in >>>> the >>>> partners' meetings and you would know that's what they are talking >>>> about. >>>> >>>> To suggest otherwise is disingenuous. >>>> >>>> And the implication that the generalist anatomic pathologist is >>>> unqualified >>>> to be signing out skins, prostates, GI's and whatever is >>>> reprehensible. >>>> This is not cardiac bypass surgery, and AP pathologists *are* trained >>>> to do >>>> all of the above. I eagerly defer to subspecialty expert consultants >>>> as >>>> needed, but most of the time they're not needed. >>>> >>>> Hospital labs that see few, if any skins, prostates, GI's, are only in >>>> that >>>> pickle because of the cherrypicking they've already been subjected to. >>>> >>>> *"in-office AP labs are an emerging frontier of employment for >>>> histologists >>>> and pathologists. In an era of high unemployment, another source of >>>> employment for our professions is "a good thing.""* >>>> >>>> Really? The jobs follow the specimens. Given the same number of >>>> specimens, >>>> there's the same number of jobs, more or less, just under different >>>> circumstances and in different locations Unless you're suggesting >>>> that >>>> in-office labs will generate increased specimens, and thus increased >>>> jobs >>>> though overutilization, i.e. excessive numbers of unnecessary biopsies >>>> and >>>> abuse of the patient and the taxpayer. In which case I have to say >>>> there's >>>> a grain of truth. And the truth hurts. And it's not " a good thing." >>>> >>>> None of this should be taken as criticism of histotechs and >>>> pathologists >>>> who find themselves working in an in-office lab. I know there's bills >>>> to >>>> pay, families to take care of, and god knows it's hard for a >>>> pathologist to >>>> find a job these days with the numbers our residency programs keep >>>> churning >>>> out (but that's another rant...). >>>> >>>> Dan Schneider, MD >>>> Amarillo, TX >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> On Mon, Apr 9, 2012 at 12:52 PM, wrote: >>>> >>>> > >>>> > Histonetters: >>>> > >>>> > In-office AP labs provide a valuable service to the practices they >>>> serve >>>> > by facilitating 1) better communication between pathologists and >>>> ordering >>>> > clinicians, 2) quality metrics that are practice-specific, and 3) >>>> high >>>> > volume, sub-specialization for both histotechnologists and >>>> pathologists. >>>> > In other words, the more of one type of histopathology a lab does >>>> (e.g., >>>> > skin, prostate, GI), the better it gets. Most people would not >>>> think >>>> of >>>> > having their cardiac bypass surgery done at a community hospital >>>> doing >>>> > 50/year; you want to go where more than 500/year are done. In >>>> > histopathology, the kinds of volume you want are in the thousands >>>> for >>>> each >>>> > tissue type. Many hospital labs do little skin or prostate >>>> histology >>>> > anymore. Many sub-specialty in-office AP labs may do thousands of >>>> cases of >>>> > one tissue type every year. >>>> > >>>> > Aside from that, in-office AP labs are an emerging frontier of >>>> employment >>>> > for histologists and pathologists. In an era of high unemployment, >>>> another >>>> > source of employment for our professions is "a good thing." >>>> > >>>> > This requirement by an insurer for accreditation will help to >>>> validate >>>> > these in-office AP labs' commitment to quality and put them on the >>>> level >>>> > with their hospital counterparts. >>>> > >>>> > John D. Cochran, MD, FCAP >>>> > >>>> > >>>> > >>>> > >>>> > >>>> > _______________________________________________ >>>> > Histonet mailing list >>>> > Histonet@lists.utsouthwestern.edu >>>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> > >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>> >>> >>> >>> -- >>> *David Costanzo, MHS, PA (ASCP)* >>> Project Manager >>> *Blufrog Path Lab Solutions* >>> 9401 Wilshire Blvd. Ste 650 >>> Beverly Hills, CA 90212 >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From amber.mckenzie <@t> gastrodocs.net Tue Apr 10 12:45:04 2012 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Apr 10 12:43:06 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation In-Reply-To: References: <344256852160910332@unknownmsgid> <1438.208.62.167.196.1334073359.squirrel@webmail.realpages.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BC11632A2F@JERRY.Gia.com> Okay, let's move on people. It's getting too personal instead of professional. Enough already -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Tuesday, April 10, 2012 12:19 PM To: Nicole Tatum; Davide Costanzo; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation I don't think it was meant as a personal attack, it's a larger conceptual issue on ethics of the business principle behind the model for in-office laboratories and the debate isn't about jobs, it's about the best interest of the patient. I am sure your skill set is exceptional. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Tuesday, April 10, 2012 11:56 AM To: Davide Costanzo; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation Rude is when you attack someone who is your equal. Yes, your right im a schmuck because I work in private practice. I didnt know that having my education, and completing my internship, and having 12yrs in the field made me a lesser histologist because I work in private practice. Seriouly get a grip. The conflict lies in you, if you cant see that we all are working to support our families. I really dont care where my fellow Histologist work, because I am happy they have a job and our professional is able to grow and that there are other opportunities for Histologist outside of hospitals. These in-house lab have created all new opportunities for Histologist and I back them 100%. Great thing about being an American, is I dont have to agree with you. This field has supported my family and allowed me to live comfortably, I will defend it for myself and others who will be entering the work force. I can only hope they have me for a mentor. I choose to promote my field and work with my collegues to ensure the survival of all of our jobs. Nicole Tatum HT ASCP You're just plain rude. Whenever someone is wrong, it is easy to > criticize others. Takes the focus off you. > > Unlike you, I will not post my personal rude comments on the entire > list serv. > > You are right, I shouldn't argue with a lesser educated schmuck either. > > Sent from my Windows Phone > From: Nicole Tatum > Sent: 4/10/2012 8:18 AM > To: Davide Costanzo; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation > Really, An undertaker. Yea, theres definately a conflict here, you. No > since in wasting my time. > > > Nicole > > > > > > > Start with reading Dr. Schneider's post. Then read Richard Cartun's >> post. Those should deal will what you are talking about very well. >> >> These in-office labs should not exist, for the very same reason the >> undertaker is no longer the ambulance driver. There is a very real, and >> significant conflict of interest. >> >> Sent from my Windows Phone >> From: Nicole Tatum >> Sent: 4/10/2012 6:45 AM >> To: Davide Costanzo; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation >> Money is at the root of all finicial decisions, in-house labs and >> hospitals. There are many over utilization of resources within the >> health >> care field. Many gallbladder surgerious are performed unneccesarly by >> general surgeous who's practice are within hospitals walls. >> Tonsilectomy. >> etc. How are those specimens not self reffered to the hospitals AP lab. >> David you made the comment about specialities staying with there >> specialty >> and not branching out. A dermatopathologist specializes in derm >> specimens >> so why is it so far fetched that he would read derm specimens from all >> sources, hospitals or in-house labs. My in-house lab has a higher turn >> around rate, lower overhead, and cuts courier fees out. We also do a >> service to our patients by allowing them one stop shopping. We can >> service >> all there needs and they do not have to have multiple appointments at >> different facilities. This cuts down on their copay and billing from >> multiple doctors. Also, it would cost more for a person to have Mohs >> surgery in a hospital setting. As we all know cost are higher at a >> hospital because they have higher overhead. The hospital is self >> reffering >> when they let a surgery center or group be affiliated with them. The >> surgery center was allowed to join the hospital so the hospital could >> reep >> the revenue generated and process their specimens. Either way, we are >> all >> joined by a common form of employment, and one facility is not better >> than >> another. My field provides jobs and creates revenue just like yours. >> Insurance company are going to make changes to try and make revenue >> during >> this change into "OBAMA CARE". Remeber we are not the enemy they are. >> Who >> are they to dictate how my company runs. Insurance companies have to >> much >> power and the decisions they force us to make do not always provide the >> best patient care. And that is the ultimate goal for any provider, to >> give >> best patient care right? This is just another hurdle we all must jump >> through in these comming changes. I vote we stick together and try our >> best to protect all our jobs. Wasnt that long ago that each of us we >> trying to get pay increases and bring the importance of our jobs to the >> fore front of pathology. The financial squeeze of the helath care system >> is going to be felt by all. Histology, pathology, radiology, cytology, >> we >> all must do our best to role with the punches and ensure quality care >> and >> our incomes, as well as our field, reguardless of location. >> >> Nicole Tatum, HT ASCP >> >> >> >> >> >> Thank you for that. How are things at Hartford Hospital? One of my >>> favorite >>> places, rotated there many years ago. Very impressive facility! Is Dr. >>> Ricci still there? >>> On Mon, Apr 9, 2012 at 4:43 PM, Richard Cartun >>> wrote: >>> >>>> This was released today. >>>> >>>> Richard >>>> >>>> Statline Special Alert: >>>> New Evidence Links Self-Referral Labs to Increased Utilization, Lower >>>> Cancer Detection Rates >>>> Study co-funded by CAP Appears in April 2012 Issue of Health Affairs >>>> April 9-Self-referring urologists billed Medicare for nearly 75% more >>>> anatomic pathology (AP) specimens compared to non self-referring >>>> physicians, according to a study published today in a leading health >>>> care policy journal. Furthermore, the study found no increase in >>>> cancer >>>> detection for the patients of self-referring physicians-in fact, the >>>> detection rate was 14% lower than that of non self-referring >>>> physicians. >>>> >>>> These findings, from an independent study co-funded by the CAP, >>>> provide >>>> the first clear evidence that self-referral of anatomic pathology >>>> services leads to increased utilization, higher Medicare spending, and >>>> lower rates of cancer detection. The study, led by renowned Georgetown >>>> University health care economist Jean Mitchell, PhD, will appear in >>>> the >>>> April 2012 issue of Health Affairs and is now available on the >>>> journal's website. >>>> >>>> >>>> ------------------------------------------------------------------------ -------- >>>> >>>> >>>> >>> Daniel Schneider 4/9/2012 4:47 PM >>> >>>> This is all about the money. The rest is rationalization. >>>> >>>> The reason a group of non-pathologist physicians opens an in-house >>>> pathology lab and hires an employee pathologist is first and foremost >>>> to harvest profit from pathology reimbursement. Be a fly on the wall >>>> in >>>> the >>>> partners' meetings and you would know that's what they are talking >>>> about. >>>> >>>> To suggest otherwise is disingenuous. >>>> >>>> And the implication that the generalist anatomic pathologist is >>>> unqualified >>>> to be signing out skins, prostates, GI's and whatever is >>>> reprehensible. >>>> This is not cardiac bypass surgery, and AP pathologists *are* trained >>>> to do >>>> all of the above. I eagerly defer to subspecialty expert consultants >>>> as >>>> needed, but most of the time they're not needed. >>>> >>>> Hospital labs that see few, if any skins, prostates, GI's, are only in >>>> that >>>> pickle because of the cherrypicking they've already been subjected to. >>>> >>>> *"in-office AP labs are an emerging frontier of employment for >>>> histologists >>>> and pathologists. In an era of high unemployment, another source of >>>> employment for our professions is "a good thing.""* >>>> >>>> Really? The jobs follow the specimens. Given the same number of >>>> specimens, >>>> there's the same number of jobs, more or less, just under different >>>> circumstances and in different locations Unless you're suggesting >>>> that >>>> in-office labs will generate increased specimens, and thus increased >>>> jobs >>>> though overutilization, i.e. excessive numbers of unnecessary biopsies >>>> and >>>> abuse of the patient and the taxpayer. In which case I have to say >>>> there's >>>> a grain of truth. And the truth hurts. And it's not " a good thing." >>>> >>>> None of this should be taken as criticism of histotechs and >>>> pathologists >>>> who find themselves working in an in-office lab. I know there's bills >>>> to >>>> pay, families to take care of, and god knows it's hard for a >>>> pathologist to >>>> find a job these days with the numbers our residency programs keep >>>> churning >>>> out (but that's another rant...). >>>> >>>> Dan Schneider, MD >>>> Amarillo, TX >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> On Mon, Apr 9, 2012 at 12:52 PM, wrote: >>>> >>>> > >>>> > Histonetters: >>>> > >>>> > In-office AP labs provide a valuable service to the practices they >>>> serve >>>> > by facilitating 1) better communication between pathologists and >>>> ordering >>>> > clinicians, 2) quality metrics that are practice-specific, and 3) >>>> high >>>> > volume, sub-specialization for both histotechnologists and >>>> pathologists. >>>> > In other words, the more of one type of histopathology a lab does >>>> (e.g., >>>> > skin, prostate, GI), the better it gets. Most people would not >>>> think >>>> of >>>> > having their cardiac bypass surgery done at a community hospital >>>> doing >>>> > 50/year; you want to go where more than 500/year are done. In >>>> > histopathology, the kinds of volume you want are in the thousands >>>> for >>>> each >>>> > tissue type. Many hospital labs do little skin or prostate >>>> histology >>>> > anymore. Many sub-specialty in-office AP labs may do thousands of >>>> cases of >>>> > one tissue type every year. >>>> > >>>> > Aside from that, in-office AP labs are an emerging frontier of >>>> employment >>>> > for histologists and pathologists. In an era of high unemployment, >>>> another >>>> > source of employment for our professions is "a good thing." >>>> > >>>> > This requirement by an insurer for accreditation will help to >>>> validate >>>> > these in-office AP labs' commitment to quality and put them on the >>>> level >>>> > with their hospital counterparts. >>>> > >>>> > John D. Cochran, MD, FCAP >>>> > >>>> > >>>> > >>>> > >>>> > >>>> > _______________________________________________ >>>> > Histonet mailing list >>>> > Histonet@lists.utsouthwestern.edu >>>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> > >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>> >>> >>> >>> -- >>> *David Costanzo, MHS, PA (ASCP)* >>> Project Manager >>> *Blufrog Path Lab Solutions* >>> 9401 Wilshire Blvd. Ste 650 >>> Beverly Hills, CA 90212 >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kaitlin <@t> prometheushealthcare.com Tue Apr 10 13:03:29 2012 From: kaitlin <@t> prometheushealthcare.com (Kaitlin Webster) Date: Tue Apr 10 13:03:38 2012 Subject: [Histonet] Histology Openings Message-ID: <000301cd1744$3c5c0150$b51403f0$@prometheushealthcare.com> Currently working on a variety of permanent, fulltime histology positions available nationwide (NY, NC, MD, AZ, CT, CO, TX FL and TN). Please feel free to e-mail me for more information- Kaitlin@prometheushealthcare.com From dlschneider <@t> gmail.com Tue Apr 10 13:21:48 2012 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Tue Apr 10 13:21:51 2012 Subject: [Histonet] In House Labs in WSJ Message-ID: The Wall Street Journal served up a timely article for us. You'll see both sides of the argument below. One side is right. DLS HEALTH INDUSTRY April 9, 2012, 7:22 p.m. ET Prostate-Test Fees Challenged By CHRISTOPHER WEAVER Doctors in urology groups that profit from tests for prostate cancer order more of them than doctors who send samples to independent laboratories, according to a study Monday in the journal Health Affairs. The study found that doctors' practices that do their own lab work bill the federal Medicare program for analyzing 72% more prostate tissue samples per biopsy while detecting fewer cases of cancer than counterparts who send specimens to outside labs. Hiring pathologists boosts revenue for a practice and creates a potential incentive to increase the number of tests ordered, said Jean Mitchell, a Georgetown University economist and author of the study. That fewer cancers were detected?21% versus 35% for those sent to external labs, according to the study?suggests "financial incentives" may play a role in decisions to order the tests, Ms. Mitchell said. Some urologists said the research doesn't necessarily indicate financial motives. Urologists in larger group practices that have in-house pathologists may be more aggressive in testing because they seek to catch cancer earlier, said Steven Schlossberg, a Yale urologist who heads a health-policy panel for the American Urological Association and wasn't involved in the research. Also, Dr. Schlossberg noted, the figures, which cover 36,261 biopsies from 2005 through 2007, are five years old. The study was financed by the College of American Pathologists and the American Clinical Laboratory Association. It is the last salvo in a turf war between laboratory companies and physician groups that have opened their own labs to conduct tests. Regulators and economists scrutinizing the growing costs of health care have targeted a range of related activities by doctors, known as self-referrals. Although a set of 1990s-era laws, named for their proponent, Rep. Pete Stark (D., Calif.), ban doctors from referring patients to most companies in which they have a financial interest, urology groups can enter the pathology business because of an exemption for certain services performed within physicians' offices. The pathologists and other groups are lobbying Congress to end the exemption. At issue in the study is a quirk of billing for lab procedures. Labs get paid based on the number of jars used to hold specimens from a prostate biopsy. Doctors can choose to put several specimens in one jar or put each in its own jar, potentially boosting lab fees, which averaged about $104 a jar in 2010, according to the study. Urologists in practices with in-house pathologists sent 11.4 jars per biopsy for testing versus 5.9 jars per biopsy for other doctors in 2005. Some doctors say that separating the samples can help them better map any cancer. In addition, urologists in recent years have been taking more samples during a biopsy to better identify the location of any cancer, said John Hollingsworth, an assistant professor of urology at the University of Michigan. The standard number of samples taken doubled to 12 over the last decade, he said. The Health Affairs study's conclusions are "largely around billing practices, not around clinical practices," said George Kwass, a pathologist based in Massachusetts and board member of the College of American Pathologists. Urologists who team up with pathologists appear to bill more, he said, leading to potential waste. Urology groups are consolidating, and increasingly moving into the pathology business. One large practice based on New York's Long Island, Integrated Medical Professionals, opened its lab in 2010 to control costs and because doctors encountered errors in outside test results, said the group's chairman, Deepak Kapoor. "We don't make a fortune on pathology," Dr. Kapoor said. But lab businesses are seeing revenue vanish. Texas pathology group ProPath stopped getting prostate tissue from large urology groups more than four years ago, said executive director Krista Crews, when these clients began doing lab work in-house. The group still gets referrals from small, one and two-doctor practices, she said. Large laboratory companies are worried about the trend, too. Quest Diagnostics Inc. DGX -2.52%said in its latest annual filings that if physicians, including urologists as well as gastroenterologists and skin and cancer doctors, continued to "internalize" testing services, it could reduce the company's sales. Write to Christopher Weaver at christopher.weaver@wsj.com Copyright 2012 Dow Jones & Company, Inc. All Rights Reserved This copy is for your personal, non-commercial use only. Distribution and use of this material are governed by our Subscriber Agreement and by copyright law. For non-personal use or to order multiple copies, please contact Dow Jones Reprints at 1-800-843-0008 or visit From pathlocums <@t> gmail.com Tue Apr 10 13:33:01 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Tue Apr 10 13:33:04 2012 Subject: [Histonet] Aetna and In-Office Lab Accreditation Message-ID: <8523242259855034415@unknownmsgid> The words used are rude, and highly uncalled for in a public forum. Not one of my posts talked about techs at all, and very wrong assumptions were made, and quite insulting posts ensued. I have great respect for techs, always have. To suggest otherwise is more wrong than I can say. Sent from my Windows Phone From: Pratt, Caroline Sent: 4/10/2012 10:18 AM To: Nicole Tatum; Davide Costanzo; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation I don't think it was meant as a personal attack, it's a larger conceptual issue on ethics of the business principle behind the model for in-office laboratories and the debate isn't about jobs, it's about the best interest of the patient. I am sure your skill set is exceptional. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Tuesday, April 10, 2012 11:56 AM To: Davide Costanzo; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation Rude is when you attack someone who is your equal. Yes, your right im a schmuck because I work in private practice. I didnt know that having my education, and completing my internship, and having 12yrs in the field made me a lesser histologist because I work in private practice. Seriouly get a grip. The conflict lies in you, if you cant see that we all are working to support our families. I really dont care where my fellow Histologist work, because I am happy they have a job and our professional is able to grow and that there are other opportunities for Histologist outside of hospitals. These in-house lab have created all new opportunities for Histologist and I back them 100%. Great thing about being an American, is I dont have to agree with you. This field has supported my family and allowed me to live comfortably, I will defend it for myself and others who will be entering the work force. I can only hope they have me for a mentor. I choose to promote my field and work with my collegues to ensure the survival of all of our jobs. Nicole Tatum HT ASCP You're just plain rude. Whenever someone is wrong, it is easy to > criticize others. Takes the focus off you. > > Unlike you, I will not post my personal rude comments on the entire > list serv. > > You are right, I shouldn't argue with a lesser educated schmuck either. > > Sent from my Windows Phone > From: Nicole Tatum > Sent: 4/10/2012 8:18 AM > To: Davide Costanzo; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Aetna and In-Office Lab Accreditation > Really, An undertaker. Yea, theres definately a conflict here, you. No > since in wasting my time. > > > Nicole > > > > > > > Start with reading Dr. Schneider's post. Then read Richard Cartun's >> post. Those should deal will what you are talking about very well. >> >> These in-office labs should not exist, for the very same reason the >> undertaker is no longer the ambulance driver. There is a very real, and >> significant conflict of interest. >> >> Sent from my Windows Phone >> From: Nicole Tatum >> Sent: 4/10/2012 6:45 AM >> To: Davide Costanzo; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation >> Money is at the root of all finicial decisions, in-house labs and >> hospitals. There are many over utilization of resources within the >> health >> care field. Many gallbladder surgerious are performed unneccesarly by >> general surgeous who's practice are within hospitals walls. >> Tonsilectomy. >> etc. How are those specimens not self reffered to the hospitals AP lab. >> David you made the comment about specialities staying with there >> specialty >> and not branching out. A dermatopathologist specializes in derm >> specimens >> so why is it so far fetched that he would read derm specimens from all >> sources, hospitals or in-house labs. My in-house lab has a higher turn >> around rate, lower overhead, and cuts courier fees out. We also do a >> service to our patients by allowing them one stop shopping. We can >> service >> all there needs and they do not have to have multiple appointments at >> different facilities. This cuts down on their copay and billing from >> multiple doctors. Also, it would cost more for a person to have Mohs >> surgery in a hospital setting. As we all know cost are higher at a >> hospital because they have higher overhead. The hospital is self >> reffering >> when they let a surgery center or group be affiliated with them. The >> surgery center was allowed to join the hospital so the hospital could >> reep >> the revenue generated and process their specimens. Either way, we are >> all >> joined by a common form of employment, and one facility is not better >> than >> another. My field provides jobs and creates revenue just like yours. >> Insurance company are going to make changes to try and make revenue >> during >> this change into "OBAMA CARE". Remeber we are not the enemy they are. >> Who >> are they to dictate how my company runs. Insurance companies have to >> much >> power and the decisions they force us to make do not always provide the >> best patient care. And that is the ultimate goal for any provider, to >> give >> best patient care right? This is just another hurdle we all must jump >> through in these comming changes. I vote we stick together and try our >> best to protect all our jobs. Wasnt that long ago that each of us we >> trying to get pay increases and bring the importance of our jobs to the >> fore front of pathology. The financial squeeze of the helath care system >> is going to be felt by all. Histology, pathology, radiology, cytology, >> we >> all must do our best to role with the punches and ensure quality care >> and >> our incomes, as well as our field, reguardless of location. >> >> Nicole Tatum, HT ASCP >> >> >> >> >> >> Thank you for that. How are things at Hartford Hospital? One of my >>> favorite >>> places, rotated there many years ago. Very impressive facility! Is Dr. >>> Ricci still there? >>> On Mon, Apr 9, 2012 at 4:43 PM, Richard Cartun >>> wrote: >>> >>>> This was released today. >>>> >>>> Richard >>>> >>>> Statline Special Alert: >>>> New Evidence Links Self-Referral Labs to Increased Utilization, Lower >>>> Cancer Detection Rates >>>> Study co-funded by CAP Appears in April 2012 Issue of Health Affairs >>>> April 9-Self-referring urologists billed Medicare for nearly 75% more >>>> anatomic pathology (AP) specimens compared to non self-referring >>>> physicians, according to a study published today in a leading health >>>> care policy journal. Furthermore, the study found no increase in >>>> cancer >>>> detection for the patients of self-referring physicians-in fact, the >>>> detection rate was 14% lower than that of non self-referring >>>> physicians. >>>> >>>> These findings, from an independent study co-funded by the CAP, >>>> provide >>>> the first clear evidence that self-referral of anatomic pathology >>>> services leads to increased utilization, higher Medicare spending, and >>>> lower rates of cancer detection. The study, led by renowned Georgetown >>>> University health care economist Jean Mitchell, PhD, will appear in >>>> the >>>> April 2012 issue of Health Affairs and is now available on the >>>> journal's website. >>>> >>>> >>>> ------------------------------------------------------------------------ -------- >>>> >>>> >>>> >>> Daniel Schneider 4/9/2012 4:47 PM >>> >>>> This is all about the money. The rest is rationalization. >>>> >>>> The reason a group of non-pathologist physicians opens an in-house >>>> pathology lab and hires an employee pathologist is first and foremost >>>> to harvest profit from pathology reimbursement. Be a fly on the wall >>>> in >>>> the >>>> partners' meetings and you would know that's what they are talking >>>> about. >>>> >>>> To suggest otherwise is disingenuous. >>>> >>>> And the implication that the generalist anatomic pathologist is >>>> unqualified >>>> to be signing out skins, prostates, GI's and whatever is >>>> reprehensible. >>>> This is not cardiac bypass surgery, and AP pathologists *are* trained >>>> to do >>>> all of the above. I eagerly defer to subspecialty expert consultants >>>> as >>>> needed, but most of the time they're not needed. >>>> >>>> Hospital labs that see few, if any skins, prostates, GI's, are only in >>>> that >>>> pickle because of the cherrypicking they've already been subjected to. >>>> >>>> *"in-office AP labs are an emerging frontier of employment for >>>> histologists >>>> and pathologists. In an era of high unemployment, another source of >>>> employment for our professions is "a good thing.""* >>>> >>>> Really? The jobs follow the specimens. Given the same number of >>>> specimens, >>>> there's the same number of jobs, more or less, just under different >>>> circumstances and in different locations Unless you're suggesting >>>> that >>>> in-office labs will generate increased specimens, and thus increased >>>> jobs >>>> though overutilization, i.e. excessive numbers of unnecessary biopsies >>>> and >>>> abuse of the patient and the taxpayer. In which case I have to say >>>> there's >>>> a grain of truth. And the truth hurts. And it's not " a good thing." >>>> >>>> None of this should be taken as criticism of histotechs and >>>> pathologists >>>> who find themselves working in an in-office lab. I know there's bills >>>> to >>>> pay, families to take care of, and god knows it's hard for a >>>> pathologist to >>>> find a job these days with the numbers our residency programs keep >>>> churning >>>> out (but that's another rant...). >>>> >>>> Dan Schneider, MD >>>> Amarillo, TX >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> >>>> On Mon, Apr 9, 2012 at 12:52 PM, wrote: >>>> >>>> > >>>> > Histonetters: >>>> > >>>> > In-office AP labs provide a valuable service to the practices they >>>> serve >>>> > by facilitating 1) better communication between pathologists and >>>> ordering >>>> > clinicians, 2) quality metrics that are practice-specific, and 3) >>>> high >>>> > volume, sub-specialization for both histotechnologists and >>>> pathologists. >>>> > In other words, the more of one type of histopathology a lab does >>>> (e.g., >>>> > skin, prostate, GI), the better it gets. Most people would not >>>> think >>>> of >>>> > having their cardiac bypass surgery done at a community hospital >>>> doing >>>> > 50/year; you want to go where more than 500/year are done. In >>>> > histopathology, the kinds of volume you want are in the thousands >>>> for >>>> each >>>> > tissue type. Many hospital labs do little skin or prostate >>>> histology >>>> > anymore. Many sub-specialty in-office AP labs may do thousands of >>>> cases of >>>> > one tissue type every year. >>>> > >>>> > Aside from that, in-office AP labs are an emerging frontier of >>>> employment >>>> > for histologists and pathologists. In an era of high unemployment, >>>> another >>>> > source of employment for our professions is "a good thing." >>>> > >>>> > This requirement by an insurer for accreditation will help to >>>> validate >>>> > these in-office AP labs' commitment to quality and put them on the >>>> level >>>> > with their hospital counterparts. >>>> > >>>> > John D. Cochran, MD, FCAP >>>> > >>>> > >>>> > >>>> > >>>> > >>>> > _______________________________________________ >>>> > Histonet mailing list >>>> > Histonet@lists.utsouthwestern.edu >>>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> > >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>> >>> >>> >>> -- >>> *David Costanzo, MHS, PA (ASCP)* >>> Project Manager >>> *Blufrog Path Lab Solutions* >>> 9401 Wilshire Blvd. Ste 650 >>> Beverly Hills, CA 90212 >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Timothy.Morken <@t> ucsfmedctr.org Tue Apr 10 13:39:06 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Apr 10 13:39:22 2012 Subject: [Histonet] In House Labs in WSJ In-Reply-To: References: Message-ID: <8D7C2D242DBD45498006B21122072BF8A4F3E89C@MCINFRWEM003.ucsfmedicalcenter.org> Not surprising since our health care system is biased to pay for tests and treatments, not results. On top of this there are serious questions as to whether the PSA screening that leads to biopsies is useful in the long term. There is a recommendation out there to stop PSA screening for most men since it is largely non-specific. That test is what leads to the biopsies. Less screening = fewer biopsies = less revenue. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider Sent: Tuesday, April 10, 2012 11:22 AM To: Histonet Subject: [Histonet] In House Labs in WSJ The Wall Street Journal served up a timely article for us. You'll see both sides of the argument below. One side is right. DLS HEALTH INDUSTRY April 9, 2012, 7:22 p.m. ET Prostate-Test Fees Challenged By CHRISTOPHER WEAVER Doctors in urology groups that profit from tests for prostate cancer order more of them than doctors who send samples to independent laboratories, according to a study Monday in the journal Health Affairs. The study found that doctors' practices that do their own lab work bill the federal Medicare program for analyzing 72% more prostate tissue samples per biopsy while detecting fewer cases of cancer than counterparts who send specimens to outside labs. Hiring pathologists boosts revenue for a practice and creates a potential incentive to increase the number of tests ordered, said Jean Mitchell, a Georgetown University economist and author of the study. That fewer cancers were detected-21% versus 35% for those sent to external labs, according to the study-suggests "financial incentives" may play a role in decisions to order the tests, Ms. Mitchell said. Some urologists said the research doesn't necessarily indicate financial motives. Urologists in larger group practices that have in-house pathologists may be more aggressive in testing because they seek to catch cancer earlier, said Steven Schlossberg, a Yale urologist who heads a health-policy panel for the American Urological Association and wasn't involved in the research. Also, Dr. Schlossberg noted, the figures, which cover 36,261 biopsies from 2005 through 2007, are five years old. The study was financed by the College of American Pathologists and the American Clinical Laboratory Association. It is the last salvo in a turf war between laboratory companies and physician groups that have opened their own labs to conduct tests. Regulators and economists scrutinizing the growing costs of health care have targeted a range of related activities by doctors, known as self-referrals. Although a set of 1990s-era laws, named for their proponent, Rep. Pete Stark (D., Calif.), ban doctors from referring patients to most companies in which they have a financial interest, urology groups can enter the pathology business because of an exemption for certain services performed within physicians' offices. The pathologists and other groups are lobbying Congress to end the exemption. At issue in the study is a quirk of billing for lab procedures. Labs get paid based on the number of jars used to hold specimens from a prostate biopsy. Doctors can choose to put several specimens in one jar or put each in its own jar, potentially boosting lab fees, which averaged about $104 a jar in 2010, according to the study. Urologists in practices with in-house pathologists sent 11.4 jars per biopsy for testing versus 5.9 jars per biopsy for other doctors in 2005. Some doctors say that separating the samples can help them better map any cancer. In addition, urologists in recent years have been taking more samples during a biopsy to better identify the location of any cancer, said John Hollingsworth, an assistant professor of urology at the University of Michigan. The standard number of samples taken doubled to 12 over the last decade, he said. The Health Affairs study's conclusions are "largely around billing practices, not around clinical practices," said George Kwass, a pathologist based in Massachusetts and board member of the College of American Pathologists. Urologists who team up with pathologists appear to bill more, he said, leading to potential waste. Urology groups are consolidating, and increasingly moving into the pathology business. One large practice based on New York's Long Island, Integrated Medical Professionals, opened its lab in 2010 to control costs and because doctors encountered errors in outside test results, said the group's chairman, Deepak Kapoor. "We don't make a fortune on pathology," Dr. Kapoor said. But lab businesses are seeing revenue vanish. Texas pathology group ProPath stopped getting prostate tissue from large urology groups more than four years ago, said executive director Krista Crews, when these clients began doing lab work in-house. The group still gets referrals from small, one and two-doctor practices, she said. Large laboratory companies are worried about the trend, too. Quest Diagnostics Inc. DGX -2.52%said in its latest annual filings that if physicians, including urologists as well as gastroenterologists and skin and cancer doctors, continued to "internalize" testing services, it could reduce the company's sales. Write to Christopher Weaver at christopher.weaver@wsj.com Copyright 2012 Dow Jones & Company, Inc. All Rights Reserved This copy is for your personal, non-commercial use only. Distribution and use of this material are governed by our Subscriber Agreement and by copyright law. For non-personal use or to order multiple copies, please contact Dow Jones Reprints at 1-800-843-0008 or visit _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Tue Apr 10 13:55:01 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Apr 10 13:55:08 2012 Subject: [Histonet] New to paraffin cutting - seeking advice In-Reply-To: References: Message-ID: I hope you're not training yourself to use a microtome. Please tell me you have an experienced cutter supervising you. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Apr 10 14:07:41 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Apr 10 14:07:48 2012 Subject: [Histonet] RE: Microwave processors In-Reply-To: References: <24B7B291CC88D04AB663958E77A1F59D02E3D6@ex09.net.ucsf.edu>, , Message-ID: I have used the Sakura and Milestone( original offerings from some time ago and also later models). The variables are changed from conventional processing, and so you have to think about different things. When I have worked places that tried to transition to MW from conventional, the trouble starts when they try to design the programs like a conventional processor. My *theory*, which is based on some research over the last 5 years and as referenced by published literature on MW use in pathology, has to do with the polarity ( molecular composition) and the water content. The effect is more molecular than physical. You do have to customize for this with more detailed programs to get the best results in my experience. Dimensions and thickness are even more important than in conventional. Once you get over the change hurdle, it works ok and saves loads of time, decreases turn around and lets you move your staff in desirable ways. There is a revision of the CLSI MW guidelines that hopefully will get out there soon. I think when this is out it will help explain and help those wanting to use MW processors to improve TAT without tissue effects. In the meantime, I just did some basic literature searches and this really cleared up my understanding of the process and has helped me with programming these instruments.Joelle Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 10 Apr 2012 12:36:00 -0400 > From: Caroline.Pratt@uphs.upenn.edu > To: jqb7@cdc.gov; Erin.Martin@ucsf.edu; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Microwave processors > CC: > > We cannot say the same, we had issues with shaves appearing "cooked". > We had specialists out several times and after many suggestions, nothing > resolved the issue. It doesn't happen consistently but it definitely > happens and we even attempted to track by tech or shifts or when the > solutions were changed and no patterns could be found after several > years. Sakura has a new vendor for reagents now and they are going to > come run some test slides but the VIP quality for derm keeps the > pathologists much happier based on our experience. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bartlett, Jeanine (CDC/OID/NCEZID) > Sent: Tuesday, April 10, 2012 12:14 PM > To: Martin, Erin; histonet > Subject: [Histonet] RE: Microwave processors > > Erin, > > We have Sakura's Xpress and skins have always turned out just fine for > us. It is very easy to use and maintain. > > Jeanine H. Bartlett > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 404-639-3590 > Jeanine.bartlett@cdc.hhs.gov > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, > Erin > Sent: Tuesday, April 10, 2012 10:38 AM > To: histonet > Subject: [Histonet] Microwave processors > > Hi histonetters! > > Our pathologists want to turn around skin biopsies same day and are > again looking at microwave processors. Due to a bad past experience, > I'm not enthused but perhaps there is someone out there who loves their > microwave processor? Even on derm? Or has anyone worked out a good > rapid derm processing protocol on a conventional processor? > > > > Thank you so much! > > Erin > > > > Erin Martin, Histology Supervisor > UCSF Dermatopathology Service > 415-353-7248 > > Confidentiality Notice > The information transmitted is intended only for the person or entity to > which it is addressed and may contain confidential and/or priviledged > material. Any review, retransmission, dissemination or other use of, or > taking of any actin in reliance upon, this information by persons or > entities other than the intended recipient is prohibited. If you > receive this in error, please contact the sender and delete the material > from any computer. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Apr 10 14:54:09 2012 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Tue Apr 10 14:54:12 2012 Subject: [Histonet] Leica Bond IHC Platform Message-ID: Use it everyday. Are you looking for "opinions"? Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Wellen Terrence D. :LPH Lab" Date: Thu, 5 Apr 2012 00:06:04 To: Subject: [Histonet] Leica Bond IHC Platform Does anyone have any experience with this product? Terrence Wellen? HT(ASCP) Legacy Good Samaritan Hospital Portland, OR _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Tue Apr 10 15:56:43 2012 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Apr 10 15:56:47 2012 Subject: [Histonet] Flammable cabinets Message-ID: <55C1FD599E1FFB479BAA2E4B5EF348C3013AC8F413BE@mmc-mail.ad.mhsil.com> Can anybody explain how much alcohol or other flammables we can store in a flammable cabinet in a room? I have read the CAP guidelines and am still confused. Do the CAP guidelines only have to do with stored reagents outside of a flammable cabinet? What am I missing? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From MSHERWOOD <@t> PARTNERS.ORG Tue Apr 10 16:29:38 2012 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Tue Apr 10 16:31:33 2012 Subject: [Histonet] RE: Flammable cabinets In-Reply-To: <55C1FD599E1FFB479BAA2E4B5EF348C3013AC8F413BE@mmc-mail.ad.mhsil.com> References: <55C1FD599E1FFB479BAA2E4B5EF348C3013AC8F413BE@mmc-mail.ad.mhsil.com> Message-ID: <090FA56107A969459F3941DDD5585C3A1162C375@PHSX10MB10.partners.org> We are a research lab and usually the restrictions apply to flammables outside the cabinet. I believe they don't want more than 100-150ml of any one flammable. I don't think there is a restriction to what's stored inside one--probably depends upon the size of the cabinet. Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, April 10, 2012 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Flammable cabinets Can anybody explain how much alcohol or other flammables we can store in a flammable cabinet in a room? I have read the CAP guidelines and am still confused. Do the CAP guidelines only have to do with stored reagents outside of a flammable cabinet? What am I missing? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From one_angel_secret <@t> yahoo.com Tue Apr 10 16:33:17 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Apr 10 16:33:24 2012 Subject: [Histonet] In House Labs in WSJ In-Reply-To: <8D7C2D242DBD45498006B21122072BF8A4F3E89C@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF8A4F3E89C@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <4ED53330-0BF5-4208-A1F5-81AB2D942FC4@yahoo.com> > Less screening = fewer biopsies = less revenue = less prostate cancers caught early = more deaths to prostate cancers. Would you not agree? And for all those advocating closure of private labs, do you also feel the same way about private pathologist owned labs who reep the benefits of getting all the out PT work from affiliated physicians while they also get a fee to serve as medical directors of hospital labs and get the pc portion of hospital work of which they can order as many test they want so they get the pc portion while the hospital gets the tc and all the big bills associated with doing the test making it hard on tax payer as well because so much in a hospital is already subsidize by the gov. Is what you really want is to have all pathologist as employees of the hospitals? And have the hospital bill global. And a few walmart like reference labs I'm just curious as to the exact position of some on here. Thanks Kim Sent from my iPhone On Apr 10, 2012, at 2:39 PM, "Morken, Timothy" wrote: > Not surprising since our health care system is biased to pay for tests and treatments, not results. On top of this there are serious questions as to whether the PSA screening that leads to biopsies is useful in the long term. There is a recommendation out there to stop PSA screening for most men since it is largely non-specific. That test is what leads to the biopsies. Less screening = fewer biopsies = less revenue. > > Tim Morken > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider > Sent: Tuesday, April 10, 2012 11:22 AM > To: Histonet > Subject: [Histonet] In House Labs in WSJ > > The Wall Street Journal served up a timely article for us. > You'll see both sides of the argument below. One side is right. > > DLS > > HEALTH INDUSTRY > April 9, 2012, 7:22 p.m. ET > Prostate-Test Fees Challenged > > By CHRISTOPHER WEAVER > Doctors in urology groups that profit from tests for prostate cancer order more of them than doctors who send samples to independent laboratories, according to a study Monday in the journal Health Affairs. > > The study found that doctors' practices that do their own lab work bill the federal Medicare program for analyzing 72% more prostate tissue samples per biopsy while detecting fewer cases of cancer than counterparts who send specimens to outside labs. > > Hiring pathologists boosts revenue for a practice and creates a potential incentive to increase the number of tests ordered, said Jean Mitchell, a Georgetown University economist and author of the study. > > That fewer cancers were detected-21% versus 35% for those sent to external labs, according to the study-suggests "financial incentives" > may play a role in decisions to order the tests, Ms. Mitchell said. > > Some urologists said the research doesn't necessarily indicate financial motives. Urologists in larger group practices that have in-house pathologists may be more aggressive in testing because they seek to catch cancer earlier, said Steven Schlossberg, a Yale urologist who heads a health-policy panel for the American Urological Association and wasn't involved in the research. Also, Dr. Schlossberg noted, the figures, which cover 36,261 biopsies from 2005 through 2007, are five years old. > > The study was financed by the College of American Pathologists and the American Clinical Laboratory Association. It is the last salvo in a turf war between laboratory companies and physician groups that have opened their own labs to conduct tests. > > Regulators and economists scrutinizing the growing costs of health care have targeted a range of related activities by doctors, known as self-referrals. > > Although a set of 1990s-era laws, named for their proponent, Rep. Pete Stark (D., Calif.), ban doctors from referring patients to most companies in which they have a financial interest, urology groups can enter the pathology business because of an exemption for certain services performed within physicians' offices. The pathologists and other groups are lobbying Congress to end the exemption. > > At issue in the study is a quirk of billing for lab procedures. Labs get paid based on the number of jars used to hold specimens from a prostate biopsy. Doctors can choose to put several specimens in one jar or put each in its own jar, potentially boosting lab fees, which averaged about $104 a jar in 2010, according to the study. > > Urologists in practices with in-house pathologists sent 11.4 jars per biopsy for testing versus 5.9 jars per biopsy for other doctors in 2005. > > > Some doctors say that separating the samples can help them better map any cancer. > > In addition, urologists in recent years have been taking more samples during a biopsy to better identify the location of any cancer, said John Hollingsworth, an assistant professor of urology at the University of Michigan. The standard number of samples taken doubled to 12 over the last decade, he said. > > The Health Affairs study's conclusions are "largely around billing practices, not around clinical practices," said George Kwass, a pathologist based in Massachusetts and board member of the College of American Pathologists. Urologists who team up with pathologists appear to bill more, he said, leading to potential waste. > > Urology groups are consolidating, and increasingly moving into the pathology business. One large practice based on New York's Long Island, Integrated Medical Professionals, opened its lab in 2010 to control costs and because doctors encountered errors in outside test results, said the group's chairman, Deepak Kapoor. > > "We don't make a fortune on pathology," Dr. Kapoor said. > > But lab businesses are seeing revenue vanish. Texas pathology group ProPath stopped getting prostate tissue from large urology groups more than four years ago, said executive director Krista Crews, when these clients began doing lab work in-house. The group still gets referrals from small, one and two-doctor practices, she said. > > Large laboratory companies are worried about the trend, too. Quest Diagnostics Inc. DGX -2.52%said in its latest annual filings that if physicians, including urologists as well as gastroenterologists and skin and cancer doctors, continued to "internalize" testing services, it could reduce the company's sales. > > Write to Christopher Weaver at christopher.weaver@wsj.com > > Copyright 2012 Dow Jones & Company, Inc. All Rights Reserved > > This copy is for your personal, non-commercial use only. Distribution and use of this material are governed by our Subscriber Agreement and by copyright law. For non-personal use or to order multiple copies, please contact Dow Jones Reprints at 1-800-843-0008 or visit _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aprescot <@t> jhmi.edu Tue Apr 10 16:52:07 2012 From: aprescot <@t> jhmi.edu (Arlene Prescott) Date: Tue Apr 10 16:52:12 2012 Subject: [Histonet] Labeling specimens in the OR Message-ID: <84994C67-38AE-4122-B180-C3F7E91E8663@jhmi.edu> Does anyone have experience with the labeling and bar coding of surgical pathology specimens in the OR? Please send your experience to aprescot@jhmi.edu From Timothy.Morken <@t> ucsfmedctr.org Tue Apr 10 16:48:32 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Apr 10 16:58:52 2012 Subject: [Histonet] In House Labs in WSJ In-Reply-To: <4ED53330-0BF5-4208-A1F5-81AB2D942FC4@yahoo.com> References: <8D7C2D242DBD45498006B21122072BF8A4F3E89C@MCINFRWEM003.ucsfmedicalcenter.org> <4ED53330-0BF5-4208-A1F5-81AB2D942FC4@yahoo.com> Message-ID: <8D7C2D242DBD45498006B21122072BF8A4F3EBCB@MCINFRWEM003.ucsfmedicalcenter.org> >From the New York Times: 1) U.S. Panel Says No to Prostate Screening for Healthy Men By GARDINER HARRIS Published: October 6, 2011 Healthy men should no longer receive a P.S.A. blood test to screen for prostate cancer because the test does not save lives over all and often leads to more tests and treatments that needlessly cause pain, impotence and incontinence in many, a key government health panel has decided. The draft recommendation, by the United States Preventive Services Task Force and due for official release next week, is based on the results of five well-controlled clinical trials and could substantially change the care given to men 50 and older. There are 44 million such men in the United States, and 33 million of them have already had a P.S.A. test - sometimes without their knowledge - during routine physicals. The task force's recommendations are followed by most medical groups. Two years ago the task force recommended that women in their 40s should no longer get routine mammograms, setting off a firestorm of controversy. The recommendation to avoid the P.S.A. test is even more forceful and applies to healthy men of all ages. "Unfortunately, the evidence now shows that this test does not save men's lives," said Dr. Virginia Moyer, a professor of pediatrics at Baylor College of Medicine and chairwoman of the task force. "This test cannot tell the difference between cancers that will and will not affect a man during his natural lifetime. We need to find one that does." Article continues.... 2) Prostate Test Found to Save Few Lives By GINA KOLATA Published: March 18, 2009 The PSA blood test, used to screen for prostate cancer, saves few lives and leads to risky and unnecessary treatments for large numbers of men, two large studies have found. Mortality Results from a Randomized Prostate-Cancer Screening Trial (The New England Journal of Medicine) Screening and Prostate-Cancer Mortality in a Randomized European Study (The New England Journal of Medicine) The findings, the first based on rigorous, randomized studies, confirm some longstanding concerns about the wisdom of widespread prostate cancer screening. Although the studies are continuing, results so far are considered significant and the most definitive to date. The PSA test, which measures a protein released by prostate cells, does what it is supposed to do - indicates a cancer might be present, leading to biopsies to determine if there is a tumor. But it has been difficult to know whether finding prostate cancer early saves lives. Most of the cancers tend to grow very slowly and are never a threat and, with the faster-growing ones, even early diagnosis might be too late. The studies - one in Europe and the other in the United States - are "some of the most important studies in the history of men's health," said Dr. Otis Brawley, the chief medical officer of the American Cancer Society. In the European study, 48 men were told they had prostate cancer and needlessly treated for it for every man whose death was prevented within a decade after having had a PSA test. Dr. Peter B. Bach, a physician and epidemiologist at Memorial Sloan-Kettering Cancer Center, says one way to think of the data is to suppose he has a PSA test today. It leads to a biopsy that reveals he has prostate cancer, and he is treated for it. There is a one in 50 chance that, in 2019 or later, he will be spared death from a cancer that would otherwise have killed him. And there is a 49 in 50 chance that he will have been treated unnecessarily for a cancer that was never a threat to his life. Article continues.... -----Original Message----- From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Tuesday, April 10, 2012 2:33 PM To: Morken, Timothy Cc: Daniel Schneider; Histonet Subject: Re: [Histonet] In House Labs in WSJ > Less screening = fewer biopsies = less revenue = less prostate cancers caught early = more deaths to prostate cancers. Would you not agree? And for all those advocating closure of private labs, do you also feel the same way about private pathologist owned labs who reep the benefits of getting all the out PT work from affiliated physicians while they also get a fee to serve as medical directors of hospital labs and get the pc portion of hospital work of which they can order as many test they want so they get the pc portion while the hospital gets the tc and all the big bills associated with doing the test making it hard on tax payer as well because so much in a hospital is already subsidize by the gov. Is what you really want is to have all pathologist as employees of the hospitals? And have the hospital bill global. And a few walmart like reference labs I'm just curious as to the exact position of some on here. Thanks Kim Sent from my iPhone On Apr 10, 2012, at 2:39 PM, "Morken, Timothy" wrote: > Not surprising since our health care system is biased to pay for tests and treatments, not results. On top of this there are serious questions as to whether the PSA screening that leads to biopsies is useful in the long term. There is a recommendation out there to stop PSA screening for most men since it is largely non-specific. That test is what leads to the biopsies. Less screening = fewer biopsies = less revenue. > > Tim Morken > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider > Sent: Tuesday, April 10, 2012 11:22 AM > To: Histonet > Subject: [Histonet] In House Labs in WSJ > > The Wall Street Journal served up a timely article for us. > You'll see both sides of the argument below. One side is right. > > DLS > > HEALTH INDUSTRY > April 9, 2012, 7:22 p.m. ET > Prostate-Test Fees Challenged > > By CHRISTOPHER WEAVER > Doctors in urology groups that profit from tests for prostate cancer order more of them than doctors who send samples to independent laboratories, according to a study Monday in the journal Health Affairs. > > The study found that doctors' practices that do their own lab work bill the federal Medicare program for analyzing 72% more prostate tissue samples per biopsy while detecting fewer cases of cancer than counterparts who send specimens to outside labs. > > Hiring pathologists boosts revenue for a practice and creates a potential incentive to increase the number of tests ordered, said Jean Mitchell, a Georgetown University economist and author of the study. > > That fewer cancers were detected-21% versus 35% for those sent to external labs, according to the study-suggests "financial incentives" > may play a role in decisions to order the tests, Ms. Mitchell said. > > Some urologists said the research doesn't necessarily indicate financial motives. Urologists in larger group practices that have in-house pathologists may be more aggressive in testing because they seek to catch cancer earlier, said Steven Schlossberg, a Yale urologist who heads a health-policy panel for the American Urological Association and wasn't involved in the research. Also, Dr. Schlossberg noted, the figures, which cover 36,261 biopsies from 2005 through 2007, are five years old. > > The study was financed by the College of American Pathologists and the American Clinical Laboratory Association. It is the last salvo in a turf war between laboratory companies and physician groups that have opened their own labs to conduct tests. > > Regulators and economists scrutinizing the growing costs of health care have targeted a range of related activities by doctors, known as self-referrals. > > Although a set of 1990s-era laws, named for their proponent, Rep. Pete Stark (D., Calif.), ban doctors from referring patients to most companies in which they have a financial interest, urology groups can enter the pathology business because of an exemption for certain services performed within physicians' offices. The pathologists and other groups are lobbying Congress to end the exemption. > > At issue in the study is a quirk of billing for lab procedures. Labs get paid based on the number of jars used to hold specimens from a prostate biopsy. Doctors can choose to put several specimens in one jar or put each in its own jar, potentially boosting lab fees, which averaged about $104 a jar in 2010, according to the study. > > Urologists in practices with in-house pathologists sent 11.4 jars per biopsy for testing versus 5.9 jars per biopsy for other doctors in 2005. > > > Some doctors say that separating the samples can help them better map any cancer. > > In addition, urologists in recent years have been taking more samples during a biopsy to better identify the location of any cancer, said John Hollingsworth, an assistant professor of urology at the University of Michigan. The standard number of samples taken doubled to 12 over the last decade, he said. > > The Health Affairs study's conclusions are "largely around billing practices, not around clinical practices," said George Kwass, a pathologist based in Massachusetts and board member of the College of American Pathologists. Urologists who team up with pathologists appear to bill more, he said, leading to potential waste. > > Urology groups are consolidating, and increasingly moving into the pathology business. One large practice based on New York's Long Island, Integrated Medical Professionals, opened its lab in 2010 to control costs and because doctors encountered errors in outside test results, said the group's chairman, Deepak Kapoor. > > "We don't make a fortune on pathology," Dr. Kapoor said. > > But lab businesses are seeing revenue vanish. Texas pathology group ProPath stopped getting prostate tissue from large urology groups more than four years ago, said executive director Krista Crews, when these clients began doing lab work in-house. The group still gets referrals from small, one and two-doctor practices, she said. > > Large laboratory companies are worried about the trend, too. Quest Diagnostics Inc. DGX -2.52%said in its latest annual filings that if physicians, including urologists as well as gastroenterologists and skin and cancer doctors, continued to "internalize" testing services, it could reduce the company's sales. > > Write to Christopher Weaver at christopher.weaver@wsj.com > > Copyright 2012 Dow Jones & Company, Inc. All Rights Reserved > > This copy is for your personal, non-commercial use only. Distribution and use of this material are governed by our Subscriber Agreement and by copyright law. For non-personal use or to order multiple copies, please contact Dow Jones Reprints at 1-800-843-0008 or visit _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Tue Apr 10 17:48:37 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Apr 10 17:48:54 2012 Subject: [Histonet] In House Labs in WSJ In-Reply-To: <8D7C2D242DBD45498006B21122072BF8A4F3EBCB@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF8A4F3E89C@MCINFRWEM003.ucsfmedicalcenter.org> <4ED53330-0BF5-4208-A1F5-81AB2D942FC4@yahoo.com> <8D7C2D242DBD45498006B21122072BF8A4F3EBCB@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A4081E@xmdb02.nch.kids> I find it interesting (and slightly amusing) that a professor of pediatrics is chairwoman of the task force on PSA testing. After my early publications on PSA IPXs, I thought that I was over that now I am in a Children's Hospital. Now I am not so sure! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, 11 April 2012 7:49 AM To: Kim Donadio Cc: Histonet Subject: RE: [Histonet] In House Labs in WSJ >From the New York Times: 1) U.S. Panel Says No to Prostate Screening for Healthy Men By GARDINER HARRIS Published: October 6, 2011 Healthy men should no longer receive a P.S.A. blood test to screen for prostate cancer because the test does not save lives over all and often leads to more tests and treatments that needlessly cause pain, impotence and incontinence in many, a key government health panel has decided. The draft recommendation, by the United States Preventive Services Task Force and due for official release next week, is based on the results of five well-controlled clinical trials and could substantially change the care given to men 50 and older. There are 44 million such men in the United States, and 33 million of them have already had a P.S.A. test - sometimes without their knowledge - during routine physicals. The task force's recommendations are followed by most medical groups. Two years ago the task force recommended that women in their 40s should no longer get routine mammograms, setting off a firestorm of controversy. The recommendation to avoid the P.S.A. test is even more forceful and applies to healthy men of all ages. "Unfortunately, the evidence now shows that this test does not save men's lives," said Dr. Virginia Moyer, a professor of pediatrics at Baylor College of Medicine and chairwoman of the task force. "This test cannot tell the difference between cancers that will and will not affect a man during his natural lifetime. We need to find one that does." Article continues.... 2) Prostate Test Found to Save Few Lives By GINA KOLATA Published: March 18, 2009 The PSA blood test, used to screen for prostate cancer, saves few lives and leads to risky and unnecessary treatments for large numbers of men, two large studies have found. Mortality Results from a Randomized Prostate-Cancer Screening Trial (The New England Journal of Medicine) Screening and Prostate-Cancer Mortality in a Randomized European Study (The New England Journal of Medicine) The findings, the first based on rigorous, randomized studies, confirm some longstanding concerns about the wisdom of widespread prostate cancer screening. Although the studies are continuing, results so far are considered significant and the most definitive to date. The PSA test, which measures a protein released by prostate cells, does what it is supposed to do - indicates a cancer might be present, leading to biopsies to determine if there is a tumor. But it has been difficult to know whether finding prostate cancer early saves lives. Most of the cancers tend to grow very slowly and are never a threat and, with the faster-growing ones, even early diagnosis might be too late. The studies - one in Europe and the other in the United States - are "some of the most important studies in the history of men's health," said Dr. Otis Brawley, the chief medical officer of the American Cancer Society. In the European study, 48 men were told they had prostate cancer and needlessly treated for it for every man whose death was prevented within a decade after having had a PSA test. Dr. Peter B. Bach, a physician and epidemiologist at Memorial Sloan-Kettering Cancer Center, says one way to think of the data is to suppose he has a PSA test today. It leads to a biopsy that reveals he has prostate cancer, and he is treated for it. There is a one in 50 chance that, in 2019 or later, he will be spared death from a cancer that would otherwise have killed him. And there is a 49 in 50 chance that he will have been treated unnecessarily for a cancer that was never a threat to his life. Article continues.... -----Original Message----- From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Tuesday, April 10, 2012 2:33 PM To: Morken, Timothy Cc: Daniel Schneider; Histonet Subject: Re: [Histonet] In House Labs in WSJ > Less screening = fewer biopsies = less revenue = less prostate cancers caught early = more deaths to prostate cancers. Would you not agree? And for all those advocating closure of private labs, do you also feel the same way about private pathologist owned labs who reep the benefits of getting all the out PT work from affiliated physicians while they also get a fee to serve as medical directors of hospital labs and get the pc portion of hospital work of which they can order as many test they want so they get the pc portion while the hospital gets the tc and all the big bills associated with doing the test making it hard on tax payer as well because so much in a hospital is already subsidize by the gov. Is what you really want is to have all pathologist as employees of the hospitals? And have the hospital bill global. And a few walmart like reference labs I'm just curious as to the exact position of some on here. Thanks Kim Sent from my iPhone On Apr 10, 2012, at 2:39 PM, "Morken, Timothy" wrote: > Not surprising since our health care system is biased to pay for tests and treatments, not results. On top of this there are serious questions as to whether the PSA screening that leads to biopsies is useful in the long term. There is a recommendation out there to stop PSA screening for most men since it is largely non-specific. That test is what leads to the biopsies. Less screening = fewer biopsies = less revenue. > > Tim Morken > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider > Sent: Tuesday, April 10, 2012 11:22 AM > To: Histonet > Subject: [Histonet] In House Labs in WSJ > > The Wall Street Journal served up a timely article for us. > You'll see both sides of the argument below. One side is right. > > DLS > > HEALTH INDUSTRY > April 9, 2012, 7:22 p.m. ET > Prostate-Test Fees Challenged > > By CHRISTOPHER WEAVER > Doctors in urology groups that profit from tests for prostate cancer order more of them than doctors who send samples to independent laboratories, according to a study Monday in the journal Health Affairs. > > The study found that doctors' practices that do their own lab work bill the federal Medicare program for analyzing 72% more prostate tissue samples per biopsy while detecting fewer cases of cancer than counterparts who send specimens to outside labs. > > Hiring pathologists boosts revenue for a practice and creates a potential incentive to increase the number of tests ordered, said Jean Mitchell, a Georgetown University economist and author of the study. > > That fewer cancers were detected-21% versus 35% for those sent to external labs, according to the study-suggests "financial incentives" > may play a role in decisions to order the tests, Ms. Mitchell said. > > Some urologists said the research doesn't necessarily indicate financial motives. Urologists in larger group practices that have in-house pathologists may be more aggressive in testing because they seek to catch cancer earlier, said Steven Schlossberg, a Yale urologist who heads a health-policy panel for the American Urological Association and wasn't involved in the research. Also, Dr. Schlossberg noted, the figures, which cover 36,261 biopsies from 2005 through 2007, are five years old. > > The study was financed by the College of American Pathologists and the American Clinical Laboratory Association. It is the last salvo in a turf war between laboratory companies and physician groups that have opened their own labs to conduct tests. > > Regulators and economists scrutinizing the growing costs of health care have targeted a range of related activities by doctors, known as self-referrals. > > Although a set of 1990s-era laws, named for their proponent, Rep. Pete Stark (D., Calif.), ban doctors from referring patients to most companies in which they have a financial interest, urology groups can enter the pathology business because of an exemption for certain services performed within physicians' offices. The pathologists and other groups are lobbying Congress to end the exemption. > > At issue in the study is a quirk of billing for lab procedures. Labs get paid based on the number of jars used to hold specimens from a prostate biopsy. Doctors can choose to put several specimens in one jar or put each in its own jar, potentially boosting lab fees, which averaged about $104 a jar in 2010, according to the study. > > Urologists in practices with in-house pathologists sent 11.4 jars per biopsy for testing versus 5.9 jars per biopsy for other doctors in 2005. > > > Some doctors say that separating the samples can help them better map any cancer. > > In addition, urologists in recent years have been taking more samples during a biopsy to better identify the location of any cancer, said John Hollingsworth, an assistant professor of urology at the University of Michigan. The standard number of samples taken doubled to 12 over the last decade, he said. > > The Health Affairs study's conclusions are "largely around billing practices, not around clinical practices," said George Kwass, a pathologist based in Massachusetts and board member of the College of American Pathologists. Urologists who team up with pathologists appear to bill more, he said, leading to potential waste. > > Urology groups are consolidating, and increasingly moving into the pathology business. One large practice based on New York's Long Island, Integrated Medical Professionals, opened its lab in 2010 to control costs and because doctors encountered errors in outside test results, said the group's chairman, Deepak Kapoor. > > "We don't make a fortune on pathology," Dr. Kapoor said. > > But lab businesses are seeing revenue vanish. Texas pathology group ProPath stopped getting prostate tissue from large urology groups more than four years ago, said executive director Krista Crews, when these clients began doing lab work in-house. The group still gets referrals from small, one and two-doctor practices, she said. > > Large laboratory companies are worried about the trend, too. Quest Diagnostics Inc. DGX -2.52%said in its latest annual filings that if physicians, including urologists as well as gastroenterologists and skin and cancer doctors, continued to "internalize" testing services, it could reduce the company's sales. > > Write to Christopher Weaver at christopher.weaver@wsj.com > > Copyright 2012 Dow Jones & Company, Inc. All Rights Reserved > > This copy is for your personal, non-commercial use only. Distribution and use of this material are governed by our Subscriber Agreement and by copyright law. For non-personal use or to order multiple copies, please contact Dow Jones Reprints at 1-800-843-0008 or visit _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From bhartologist <@t> gmail.com Tue Apr 10 17:49:47 2012 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Tue Apr 10 17:49:51 2012 Subject: [Histonet] ASCP Exam Message-ID: Hello fellow histonetters!!! I have begun studying for the ASCP HT exam. Any guidance/studying suggestions/study booklets/tactics you can throw out at me would be greatly appreciated. Any recent exam takers out there? Oh yeah, and also, since I am planning on relocating to California, does anyone know if that state has it's own state licensure? Thanks again!! -Bharti Parihar From one_angel_secret <@t> yahoo.com Tue Apr 10 17:56:10 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Apr 10 17:56:17 2012 Subject: [Histonet] In House Labs in WSJ In-Reply-To: <8D7C2D242DBD45498006B21122072BF8A4F3EBCB@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF8A4F3E89C@MCINFRWEM003.ucsfmedicalcenter.org> <4ED53330-0BF5-4208-A1F5-81AB2D942FC4@yahoo.com> <8D7C2D242DBD45498006B21122072BF8A4F3EBCB@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: A key government health panel has decided it says. Ok. I give. Sent from my iPhone On Apr 10, 2012, at 5:48 PM, "Morken, Timothy" wrote: > From the New York Times: > > 1) > U.S. Panel Says No to Prostate Screening for Healthy Men > By GARDINER HARRIS > Published: October 6, 2011 > > > Healthy men should no longer receive a P.S.A. blood test to screen for prostate cancer because the test does not save lives over all and often leads to more tests and treatments that needlessly cause pain, impotence and incontinence in many, a key government health panel has decided. > > The draft recommendation, by the United States Preventive Services Task Force and due for official release next week, is based on the results of five well-controlled clinical trials and could substantially change the care given to men 50 and older. There are 44 million such men in the United States, and 33 million of them have already had a P.S.A. test - sometimes without their knowledge - during routine physicals. > > The task force's recommendations are followed by most medical groups. Two years ago the task force recommended that women in their 40s should no longer get routine mammograms, setting off a firestorm of controversy. The recommendation to avoid the P.S.A. test is even more forceful and applies to healthy men of all ages. > > "Unfortunately, the evidence now shows that this test does not save men's lives," said Dr. Virginia Moyer, a professor of pediatrics at Baylor College of Medicine and chairwoman of the task force. "This test cannot tell the difference between cancers that will and will not affect a man during his natural lifetime. We need to find one that does." > > Article continues.... > > > 2) > > > Prostate Test Found to Save Few Lives > By GINA KOLATA > Published: March 18, 2009 > The PSA blood test, used to screen for prostate cancer, saves few lives and leads to risky and unnecessary treatments for large numbers of men, two large studies have found. > > > Mortality Results from a Randomized Prostate-Cancer Screening Trial (The New England Journal of Medicine) > Screening and Prostate-Cancer Mortality in a Randomized European Study (The New England Journal of Medicine) > > > The findings, the first based on rigorous, randomized studies, confirm some longstanding concerns about the wisdom of widespread prostate cancer screening. Although the studies are continuing, results so far are considered significant and the most definitive to date. > > The PSA test, which measures a protein released by prostate cells, does what it is supposed to do - indicates a cancer might be present, leading to biopsies to determine if there is a tumor. But it has been difficult to know whether finding prostate cancer early saves lives. Most of the cancers tend to grow very slowly and are never a threat and, with the faster-growing ones, even early diagnosis might be too late. > > The studies - one in Europe and the other in the United States - are "some of the most important studies in the history of men's health," said Dr. Otis Brawley, the chief medical officer of the American Cancer Society. > > In the European study, 48 men were told they had prostate cancer and needlessly treated for it for every man whose death was prevented within a decade after having had a PSA test. > > Dr. Peter B. Bach, a physician and epidemiologist at Memorial Sloan-Kettering Cancer Center, says one way to think of the data is to suppose he has a PSA test today. It leads to a biopsy that reveals he has prostate cancer, and he is treated for it. There is a one in 50 chance that, in 2019 or later, he will be spared death from a cancer that would otherwise have killed him. And there is a 49 in 50 chance that he will have been treated unnecessarily for a cancer that was never a threat to his life. > > Article continues.... > > -----Original Message----- > From: Kim Donadio [mailto:one_angel_secret@yahoo.com] > Sent: Tuesday, April 10, 2012 2:33 PM > To: Morken, Timothy > Cc: Daniel Schneider; Histonet > Subject: Re: [Histonet] In House Labs in WSJ > >> Less screening = fewer biopsies = less revenue = less prostate cancers caught early = more deaths to prostate cancers. > > Would you not agree? > > And for all those advocating closure of private labs, do you also feel the same way about private pathologist owned labs who reep the benefits of getting all the out PT work from affiliated physicians while they also get a fee to serve as medical directors of hospital labs and get the pc portion of hospital work of which they can order as many test they want so they get the pc portion while the hospital gets the tc and all the big bills associated with doing the test making it hard on tax payer as well because so much in a hospital is already subsidize by the gov. > > Is what you really want is to have all pathologist as employees of the hospitals? And have the hospital bill global. > > And a few walmart like reference labs > > I'm just curious as to the exact position of some on here. > > Thanks > > Kim > Sent from my iPhone > > On Apr 10, 2012, at 2:39 PM, "Morken, Timothy" wrote: > >> Not surprising since our health care system is biased to pay for tests and treatments, not results. On top of this there are serious questions as to whether the PSA screening that leads to biopsies is useful in the long term. There is a recommendation out there to stop PSA screening for most men since it is largely non-specific. That test is what leads to the biopsies. Less screening = fewer biopsies = less revenue. >> >> Tim Morken >> >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider >> Sent: Tuesday, April 10, 2012 11:22 AM >> To: Histonet >> Subject: [Histonet] In House Labs in WSJ >> >> The Wall Street Journal served up a timely article for us. >> You'll see both sides of the argument below. One side is right. >> >> DLS >> >> HEALTH INDUSTRY >> April 9, 2012, 7:22 p.m. ET >> Prostate-Test Fees Challenged >> >> By CHRISTOPHER WEAVER >> Doctors in urology groups that profit from tests for prostate cancer order more of them than doctors who send samples to independent laboratories, according to a study Monday in the journal Health Affairs. >> >> The study found that doctors' practices that do their own lab work bill the federal Medicare program for analyzing 72% more prostate tissue samples per biopsy while detecting fewer cases of cancer than counterparts who send specimens to outside labs. >> >> Hiring pathologists boosts revenue for a practice and creates a potential incentive to increase the number of tests ordered, said Jean Mitchell, a Georgetown University economist and author of the study. >> >> That fewer cancers were detected-21% versus 35% for those sent to external labs, according to the study-suggests "financial incentives" >> may play a role in decisions to order the tests, Ms. Mitchell said. >> >> Some urologists said the research doesn't necessarily indicate financial motives. Urologists in larger group practices that have in-house pathologists may be more aggressive in testing because they seek to catch cancer earlier, said Steven Schlossberg, a Yale urologist who heads a health-policy panel for the American Urological Association and wasn't involved in the research. Also, Dr. Schlossberg noted, the figures, which cover 36,261 biopsies from 2005 through 2007, are five years old. >> >> The study was financed by the College of American Pathologists and the American Clinical Laboratory Association. It is the last salvo in a turf war between laboratory companies and physician groups that have opened their own labs to conduct tests. >> >> Regulators and economists scrutinizing the growing costs of health care have targeted a range of related activities by doctors, known as self-referrals. >> >> Although a set of 1990s-era laws, named for their proponent, Rep. Pete Stark (D., Calif.), ban doctors from referring patients to most companies in which they have a financial interest, urology groups can enter the pathology business because of an exemption for certain services performed within physicians' offices. The pathologists and other groups are lobbying Congress to end the exemption. >> >> At issue in the study is a quirk of billing for lab procedures. Labs get paid based on the number of jars used to hold specimens from a prostate biopsy. Doctors can choose to put several specimens in one jar or put each in its own jar, potentially boosting lab fees, which averaged about $104 a jar in 2010, according to the study. >> >> Urologists in practices with in-house pathologists sent 11.4 jars per biopsy for testing versus 5.9 jars per biopsy for other doctors in 2005. >> >> >> Some doctors say that separating the samples can help them better map any cancer. >> >> In addition, urologists in recent years have been taking more samples during a biopsy to better identify the location of any cancer, said John Hollingsworth, an assistant professor of urology at the University of Michigan. The standard number of samples taken doubled to 12 over the last decade, he said. >> >> The Health Affairs study's conclusions are "largely around billing practices, not around clinical practices," said George Kwass, a pathologist based in Massachusetts and board member of the College of American Pathologists. Urologists who team up with pathologists appear to bill more, he said, leading to potential waste. >> >> Urology groups are consolidating, and increasingly moving into the pathology business. One large practice based on New York's Long Island, Integrated Medical Professionals, opened its lab in 2010 to control costs and because doctors encountered errors in outside test results, said the group's chairman, Deepak Kapoor. >> >> "We don't make a fortune on pathology," Dr. Kapoor said. >> >> But lab businesses are seeing revenue vanish. Texas pathology group ProPath stopped getting prostate tissue from large urology groups more than four years ago, said executive director Krista Crews, when these clients began doing lab work in-house. The group still gets referrals from small, one and two-doctor practices, she said. >> >> Large laboratory companies are worried about the trend, too. Quest Diagnostics Inc. DGX -2.52%said in its latest annual filings that if physicians, including urologists as well as gastroenterologists and skin and cancer doctors, continued to "internalize" testing services, it could reduce the company's sales. >> >> Write to Christopher Weaver at christopher.weaver@wsj.com >> >> Copyright 2012 Dow Jones & Company, Inc. All Rights Reserved >> >> This copy is for your personal, non-commercial use only. Distribution and use of this material are governed by our Subscriber Agreement and by copyright law. For non-personal use or to order multiple copies, please contact Dow Jones Reprints at 1-800-843-0008 or visit _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From one_angel_secret <@t> yahoo.com Tue Apr 10 18:03:15 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Apr 10 18:03:22 2012 Subject: [Histonet] Labeling specimens in the OR In-Reply-To: <84994C67-38AE-4122-B180-C3F7E91E8663@jhmi.edu> References: <84994C67-38AE-4122-B180-C3F7E91E8663@jhmi.edu> Message-ID: If you want barcodes on tour specimens directly from the or. Have your HIS system interfaced with your pathology information system. That way when path gets your specimen they just scan the bar code and the patients data drops into thier path system. Depending on the system you get path should be able to track specimens coming to them. My favorite system I've used so far has been Cerner copath. It was pretty easy to use. I'm sure there are other good ones though. Hope this helps Kim D Sent from my iPhone On Apr 10, 2012, at 5:52 PM, Arlene Prescott wrote: > Does anyone have experience with the labeling and bar coding of surgical pathology specimens in the OR? > > Please send your experience to aprescot@jhmi.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From meryl50 <@t> hotmail.com Tue Apr 10 18:14:32 2012 From: meryl50 <@t> hotmail.com (Meryl Roberts) Date: Tue Apr 10 18:14:35 2012 Subject: [Histonet] Processing Autopsies Message-ID: Our lab processes a high number of autopsies; however we always seem to have tissue that needs to be reprocessed; particularly brains. Does anyone out there have any suggestions as to what an optimal processing cycle would be? We are finding it hard to find a happy medium as there always seems to be something that is underprocessed, or sometimes even overprocessed. Thanks. From one_angel_secret <@t> yahoo.com Tue Apr 10 18:25:07 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Apr 10 18:25:12 2012 Subject: [Histonet] Processing Autopsies In-Reply-To: References: Message-ID: <27AD5BCA-C0BF-428B-ACA6-B584C312BEA0@yahoo.com> Brains in particular need to be fixed real well If it's a whole brain what I've done is hang the brain by a mesh or strings into a large brain bucket so it's not touching the sides or bottom. Fix for few days then get you sections. I'd go textbook on the 3 mm thick sections for processing and don't over process that will cause them to be friable. Hate that. Try a few blocks a couple different ways and what kind of alcohol are you using? Reagent grade is fine. For processing well fixed brain I've had good success with a straight 30 min for every thing. Hope this helps Kim D I'm out :) Sent from my iPhone On Apr 10, 2012, at 7:14 PM, Meryl Roberts wrote: > > > > Our lab processes a high number of autopsies; however we always seem to have tissue that needs to be reprocessed; particularly brains. Does anyone out there have any suggestions as to what an optimal processing cycle would be? We are finding it hard to find a happy medium as there always seems to be something that is underprocessed, or sometimes even overprocessed. Thanks. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JSCHUMA1 <@t> Fairview.org Tue Apr 10 18:46:26 2012 From: JSCHUMA1 <@t> Fairview.org (Schumacher, Jennifer J) Date: Tue Apr 10 18:46:32 2012 Subject: [Histonet] RE: Flammable cabinets In-Reply-To: <090FA56107A969459F3941DDD5585C3A1162C375@PHSX10MB10.partners.org> References: <55C1FD599E1FFB479BAA2E4B5EF348C3013AC8F413BE@mmc-mail.ad.mhsil.com> <090FA56107A969459F3941DDD5585C3A1162C375@PHSX10MB10.partners.org> Message-ID: <45EBFA1E7C931E45BBA7172D42F23C9209BE72FB42@EXCH-MBX5.Fairview.org> In my experience, restrictions are determined by fire codes and "zones". I would talk to your safety officer or facilities, or a local fire marshal. Jennifer Jennifer Schumacher, MA, HTL (ASCP) Hematopathology Supervisor I University of Minnesota Medical Center, Fairview I Phone 612-273-3229 I Fax 612-624-6662 I Pager 612-899-9295 I Address L227-2 MMC 198, 420 Delaware St SE, Minneapolis, MN 55455 I Email jschuma1@fairview.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Tuesday, April 10, 2012 4:30 PM To: 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Flammable cabinets We are a research lab and usually the restrictions apply to flammables outside the cabinet. I believe they don't want more than 100-150ml of any one flammable. I don't think there is a restriction to what's stored inside one--probably depends upon the size of the cabinet. Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, April 10, 2012 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Flammable cabinets Can anybody explain how much alcohol or other flammables we can store in a flammable cabinet in a room? I have read the CAP guidelines and am still confused. Do the CAP guidelines only have to do with stored reagents outside of a flammable cabinet? What am I missing? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathlocums <@t> gmail.com Tue Apr 10 19:30:59 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Tue Apr 10 19:31:03 2012 Subject: [Histonet] In House Labs in WSJ In-Reply-To: <4ED53330-0BF5-4208-A1F5-81AB2D942FC4@yahoo.com> References: <8D7C2D242DBD45498006B21122072BF8A4F3E89C@MCINFRWEM003.ucsfmedicalcenter.org> <4ED53330-0BF5-4208-A1F5-81AB2D942FC4@yahoo.com> Message-ID: > > > Less screening = fewer biopsies = less revenue = less prostate cancers > caught early = more deaths to prostate cancers. > > Would you not agree? > According to the study referenced earlier, just the opposite is true. Increased utilization arising from in-house laboratories has proven to be less effective, and much more costly than their traditional counterparts. No benefit to the patient at all, actually a detriment. The best results still come from outfits owned and operated by pathologists and/or hospitals, and at a significantly lower cost. > > And for all those advocating closure of private labs, do you also feel the > same way about private pathologist owned labs who reep the benefits of > getting all the out PT work from affiliated physicians while they also get > a fee to serve as medical directors of hospital labs and get the pc portion > of hospital work of which they can order as many test they want so they get > the pc portion while the hospital gets the tc and all the big bills > associated with doing the test making it hard on tax payer as well because > so much in a hospital is already subsidize by the gov. > Private labs outside of the hospital, owned by pathologists, do not represent the group of non-pathologist owned in-office labs we have discussed. There are no complaints arising over pure pathology labs, operated by pathologists. The complaints are in reference to private labs within a GI clinic, or in a urologists' office, etc. > > Is what you really want is to have all pathologist as employees of the > hospitals? And have the hospital bill global. > Doctors in hospital settings are very rarely employed by the hospital, with the exception being academia. In most cases, the pathology group handles their own billing for professional fees. Just like radiologists, surgeons, anaesthesiologists and most others working in a hospital are not employed by that hospital. > > And a few walmart like reference labs > > I'm just curious as to the exact position of some on here. > > Thanks > > Kim > Sent from my iPhone > > On Apr 10, 2012, at 2:39 PM, "Morken, Timothy" < > Timothy.Morken@ucsfmedctr.org> wrote: > > > Not surprising since our health care system is biased to pay for tests > and treatments, not results. On top of this there are serious questions as > to whether the PSA screening that leads to biopsies is useful in the long > term. There is a recommendation out there to stop PSA screening for most > men since it is largely non-specific. That test is what leads to the > biopsies. Less screening = fewer biopsies = less revenue. > > > > Tim Morken > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider > > Sent: Tuesday, April 10, 2012 11:22 AM > > To: Histonet > > Subject: [Histonet] In House Labs in WSJ > > > > The Wall Street Journal served up a timely article for us. > > You'll see both sides of the argument below. One side is right. > > > > DLS > > > > HEALTH INDUSTRY > > April 9, 2012, 7:22 p.m. ET > > Prostate-Test Fees Challenged > > > > By CHRISTOPHER WEAVER > > Doctors in urology groups that profit from tests for prostate cancer > order more of them than doctors who send samples to independent > laboratories, according to a study Monday in the journal Health Affairs. > > > > The study found that doctors' practices that do their own lab work bill > the federal Medicare program for analyzing 72% more prostate tissue samples > per biopsy while detecting fewer cases of cancer than counterparts who send > specimens to outside labs. > > > > Hiring pathologists boosts revenue for a practice and creates a > potential incentive to increase the number of tests ordered, said Jean > Mitchell, a Georgetown University economist and author of the study. > > > > That fewer cancers were detected-21% versus 35% for those sent to > external labs, according to the study-suggests "financial incentives" > > may play a role in decisions to order the tests, Ms. Mitchell said. > > > > Some urologists said the research doesn't necessarily indicate financial > motives. Urologists in larger group practices that have in-house > pathologists may be more aggressive in testing because they seek to catch > cancer earlier, said Steven Schlossberg, a Yale urologist who heads a > health-policy panel for the American Urological Association and wasn't > involved in the research. Also, Dr. Schlossberg noted, the figures, which > cover 36,261 biopsies from 2005 through 2007, are five years old. > > > > The study was financed by the College of American Pathologists and the > American Clinical Laboratory Association. It is the last salvo in a turf > war between laboratory companies and physician groups that have opened > their own labs to conduct tests. > > > > Regulators and economists scrutinizing the growing costs of health care > have targeted a range of related activities by doctors, known as > self-referrals. > > > > Although a set of 1990s-era laws, named for their proponent, Rep. Pete > Stark (D., Calif.), ban doctors from referring patients to most companies > in which they have a financial interest, urology groups can enter the > pathology business because of an exemption for certain services performed > within physicians' offices. The pathologists and other groups are lobbying > Congress to end the exemption. > > > > At issue in the study is a quirk of billing for lab procedures. Labs get > paid based on the number of jars used to hold specimens from a prostate > biopsy. Doctors can choose to put several specimens in one jar or put each > in its own jar, potentially boosting lab fees, which averaged about $104 a > jar in 2010, according to the study. > > > > Urologists in practices with in-house pathologists sent 11.4 jars per > biopsy for testing versus 5.9 jars per biopsy for other doctors in 2005. > > > > > > Some doctors say that separating the samples can help them better map > any cancer. > > > > In addition, urologists in recent years have been taking more samples > during a biopsy to better identify the location of any cancer, said John > Hollingsworth, an assistant professor of urology at the University of > Michigan. The standard number of samples taken doubled to 12 over the last > decade, he said. > > > > The Health Affairs study's conclusions are "largely around billing > practices, not around clinical practices," said George Kwass, a pathologist > based in Massachusetts and board member of the College of American > Pathologists. Urologists who team up with pathologists appear to bill more, > he said, leading to potential waste. > > > > Urology groups are consolidating, and increasingly moving into the > pathology business. One large practice based on New York's Long Island, > Integrated Medical Professionals, opened its lab in 2010 to control costs > and because doctors encountered errors in outside test results, said the > group's chairman, Deepak Kapoor. > > > > "We don't make a fortune on pathology," Dr. Kapoor said. > > > > But lab businesses are seeing revenue vanish. Texas pathology group > ProPath stopped getting prostate tissue from large urology groups more than > four years ago, said executive director Krista Crews, when these clients > began doing lab work in-house. The group still gets referrals from small, > one and two-doctor practices, she said. > > > > Large laboratory companies are worried about the trend, too. Quest > Diagnostics Inc. DGX -2.52%said in its latest annual filings that if > physicians, including urologists as well as gastroenterologists and skin > and cancer doctors, continued to "internalize" testing services, it could > reduce the company's sales. > > > > Write to Christopher Weaver at christopher.weaver@wsj.com > > > > Copyright 2012 Dow Jones & Company, Inc. All Rights Reserved > > > > This copy is for your personal, non-commercial use only. Distribution > and use of this material are governed by our Subscriber Agreement and by > copyright law. For non-personal use or to order multiple copies, please > contact Dow Jones Reprints at 1-800-843-0008 or visit > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From elciba <@t> hotmail.com Tue Apr 10 19:41:46 2012 From: elciba <@t> hotmail.com (ricky hachy) Date: Tue Apr 10 19:41:49 2012 Subject: [Histonet] TBS ATP1 Message-ID: Hello everybody, I am looking for the SERVICE MANUAL for the Tissue Processor TBS ATP1 . Could anyone help me . RegardsRicky From jsjurczak <@t> comcast.net Tue Apr 10 20:13:06 2012 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Tue Apr 10 20:13:14 2012 Subject: [Histonet] Histobath In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E236E7D@evcspmbx3.ads.northwestern.edu> Message-ID: <1708146692.1061665.1334106786714.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Clini -RF from Hacker is nice cuz it sits on the floor next to the cryostat at working height. Gets a lot colder too. ----- Original Message ----- From: "Bernice Frederick" To: "Patsy Ruegg" , "Margaret' 'Sherwood" , "Marilyn A Weiss" , histonet@lists.utsouthwestern.edu Sent: Monday, April 9, 2012 7:45:38 AM Subject: RE: [Histonet] Histobath Fisher owns Shandon. Part of Thermo-fisher. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Sunday, April 08, 2012 2:16 PM To: 'Sherwood, Margaret'; Marilyn.A.Weiss@kp.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histobath Is Shandon still around, I never see them anymore? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Friday, April 06, 2012 12:52 PM To: 'Marilyn.A.Weiss@kp.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histobath I googled Histobath and Shandon sells them, plus some other on-line companies. ?Check it out. Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn.A.Weiss@kp.org Sent: Friday, April 06, 2012 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histobath We are desperately looking for a Histobath. I know they do not make them anymore but if someone has a old one they are not using or a company can get their hands on one, we would be eternally ?grateful. Our Lab Manager would prefer we do not us Liquid Nitrogen. We love the Histobaths we have now. Marilyn Weiss HT (ASCP) cm Kaiser Permanente Hospital San Diego, Ca Marilyn.A.Weiss@kp.org NOTICE TO RECIPIENT: ?If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. ?If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. ?Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmhernandez78 <@t> me.com Tue Apr 10 21:48:54 2012 From: cmhernandez78 <@t> me.com (Carlos Hernandez) Date: Tue Apr 10 21:49:24 2012 Subject: [Histonet] RE: Microwave processors In-Reply-To: References: <24B7B291CC88D04AB663958E77A1F59D02E3D6@ex09.net.ucsf.edu> Message-ID: I use the Milestone Pathos Delta in my Dermpath lab and have had amazing results. As long as you follow their recommendations for processing times it works out perfectly. I am able to do small biopsies in as little as an hour and twenty minutes or big excisions in 3-4 hours with great results. Obviously I prefer Milestone over Pathos, but a couple of big reasons is because you are not limited to 3mm sections, you can process big and small tissue together using times for big tissue and it will not destroy your small biopsies, it's completely automated(no fixing and pre-process solution before loading on processor and you are not REQUIRED to use their proprietary reagents(although they are really good as well). This is just my personal opinion from using it in a Derm only practice. One last thing is that the customer service and attention they give to the customer is second to none. Hope this helps! Good luck!! Carlos On Apr 10, 2012, at 1:07 PM, joelle weaver wrote: > > I have used the Sakura and Milestone( original offerings from some time ago and also later models). The variables are changed from conventional processing, and so you have to think about different things. When I have worked places that tried to transition to MW from conventional, the trouble starts when they try to design the programs like a conventional processor. My *theory*, which is based on some research over the last 5 years and as referenced by published literature on MW use in pathology, has to do with the polarity ( molecular composition) and the water content. The effect is more molecular than physical. You do have to customize for this with more detailed programs to get the best results in my experience. Dimensions and thickness are even more important than in conventional. Once you get over the change hurdle, it works ok and saves loads of time, decreases turn around and lets you move your staff in desirable ways. There is a revision of the CLSI MW guidelines that hopefully will get out there soon. I think when this is out it will help explain and help those wanting to use MW processors to improve TAT without tissue effects. In the meantime, I just did some basic literature searches and this really cleared up my understanding of the process and has helped me with programming these instruments.Joelle > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC >> Date: Tue, 10 Apr 2012 12:36:00 -0400 >> From: Caroline.Pratt@uphs.upenn.edu >> To: jqb7@cdc.gov; Erin.Martin@ucsf.edu; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] RE: Microwave processors >> CC: >> >> We cannot say the same, we had issues with shaves appearing "cooked". >> We had specialists out several times and after many suggestions, nothing >> resolved the issue. It doesn't happen consistently but it definitely >> happens and we even attempted to track by tech or shifts or when the >> solutions were changed and no patterns could be found after several >> years. Sakura has a new vendor for reagents now and they are going to >> come run some test slides but the VIP quality for derm keeps the >> pathologists much happier based on our experience. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Bartlett, Jeanine (CDC/OID/NCEZID) >> Sent: Tuesday, April 10, 2012 12:14 PM >> To: Martin, Erin; histonet >> Subject: [Histonet] RE: Microwave processors >> >> Erin, >> >> We have Sakura's Xpress and skins have always turned out just fine for >> us. It is very easy to use and maintain. >> >> Jeanine H. Bartlett >> Centers for Disease Control and Prevention >> Infectious Diseases Pathology Branch >> 404-639-3590 >> Jeanine.bartlett@cdc.hhs.gov >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, >> Erin >> Sent: Tuesday, April 10, 2012 10:38 AM >> To: histonet >> Subject: [Histonet] Microwave processors >> >> Hi histonetters! >> >> Our pathologists want to turn around skin biopsies same day and are >> again looking at microwave processors. Due to a bad past experience, >> I'm not enthused but perhaps there is someone out there who loves their >> microwave processor? Even on derm? Or has anyone worked out a good >> rapid derm processing protocol on a conventional processor? >> >> >> >> Thank you so much! >> >> Erin >> >> >> >> Erin Martin, Histology Supervisor >> UCSF Dermatopathology Service >> 415-353-7248 >> >> Confidentiality Notice >> The information transmitted is intended only for the person or entity to >> which it is addressed and may contain confidential and/or priviledged >> material. Any review, retransmission, dissemination or other use of, or >> taking of any actin in reliance upon, this information by persons or >> entities other than the intended recipient is prohibited. If you >> receive this in error, please contact the sender and delete the material >> from any computer. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Apr 10 22:38:00 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Apr 10 22:38:56 2012 Subject: [Histonet] ASCP Exam In-Reply-To: Message-ID: California does not have state licensure for histotechs. There is no regulation that histotechs even have to be certified, but many of the facilities will only hire certified technicians. The NSH has a list of study materials at http://www.nsh.org/content/certification-exam-study-aids Bharti Parihar Sent by: histonet-bounces@lists.utsouthwestern.edu 04/10/2012 03:50 PM To Histonet Archive cc Subject [Histonet] ASCP Exam Hello fellow histonetters!!! I have begun studying for the ASCP HT exam. Any guidance/studying suggestions/study booklets/tactics you can throw out at me would be greatly appreciated. Any recent exam takers out there? Oh yeah, and also, since I am planning on relocating to California, does anyone know if that state has it's own state licensure? Thanks again!! -Bharti Parihar _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Wed Apr 11 05:02:32 2012 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Apr 11 05:02:40 2012 Subject: [Histonet] RE: Flammable cabinets In-Reply-To: <55C1FD599E1FFB479BAA2E4B5EF348C3013AC8F413BE@mmc-mail.ad.mhsil.com> References: <55C1FD599E1FFB479BAA2E4B5EF348C3013AC8F413BE@mmc-mail.ad.mhsil.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FB8D59EF@LRGHEXVS1.practice.lrgh.org> Depends of the amount the cabinet is rated for. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, April 10, 2012 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Flammable cabinets Can anybody explain how much alcohol or other flammables we can store in a flammable cabinet in a room? I have read the CAP guidelines and am still confused. Do the CAP guidelines only have to do with stored reagents outside of a flammable cabinet? What am I missing? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From relia1 <@t> earthlink.net Wed Apr 11 08:59:08 2012 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Apr 11 08:59:14 2012 Subject: [Histonet] RELIA Hot Histology Job Alert - Histotech needed in Denver, CO. Can you help? Message-ID: <397B347D090942EEB430152FD359BDC4@ownerf1abaad51> Hi Histonetters. I hope everyone is having a great day. I am excited to tell you about an opportunity that I have with a client in Denver, CO This is a 32 hour per week permanent day shift position and my client offers a competitive salary, nice benefits and a great group of people to work with. They need someone who is ASCP certified and has at least 2 years of histology experience including some IHC . I have heard great things about this facility and would love to introduce you to them. If you would like more information please contact me toll free at 866-607-3542 or by email at relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From mcauliff <@t> umdnj.edu Wed Apr 11 09:22:04 2012 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Apr 11 09:20:54 2012 Subject: [Histonet] In House Labs in WSJ In-Reply-To: <4ED53330-0BF5-4208-A1F5-81AB2D942FC4@yahoo.com> References: <8D7C2D242DBD45498006B21122072BF8A4F3E89C@MCINFRWEM003.ucsfmedicalcenter.org> <4ED53330-0BF5-4208-A1F5-81AB2D942FC4@yahoo.com> Message-ID: <4F85938C.5090701@umdnj.edu> On 4/10/2012 5:33 PM, Kim Donadio wrote: >> Less screening = fewer biopsies = less revenue = less prostate cancers caught early = more deaths to prostate cancers. > Would you not agree? No. There is very good scientific evidence that screening does not increase survival rates but it does drive up costs and unnecessary surgery and related complications. I can send the papers from NEJM if you like. Geoff > > And for all those advocating closure of private labs, do you also feel the same way about private pathologist owned labs who reep the benefits of getting all the out PT work from affiliated physicians while they also get a fee to serve as medical directors of hospital labs and get the pc portion of hospital work of which they can order as many test they want so they get the pc portion while the hospital gets the tc and all the big bills associated with doing the test making it hard on tax payer as well because so much in a hospital is already subsidize by the gov. > > Is what you really want is to have all pathologist as employees of the hospitals? And have the hospital bill global. > > And a few walmart like reference labs > > I'm just curious as to the exact position of some on here. > > Thanks > > Kim > Sent from my iPhone > > On Apr 10, 2012, at 2:39 PM, "Morken, Timothy" wrote: > >> Not surprising since our health care system is biased to pay for tests and treatments, not results. On top of this there are serious questions as to whether the PSA screening that leads to biopsies is useful in the long term. There is a recommendation out there to stop PSA screening for most men since it is largely non-specific. That test is what leads to the biopsies. Less screening = fewer biopsies = less revenue. >> >> Tim Morken >> >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider >> Sent: Tuesday, April 10, 2012 11:22 AM >> To: Histonet >> Subject: [Histonet] In House Labs in WSJ >> >> The Wall Street Journal served up a timely article for us. >> You'll see both sides of the argument below. One side is right. >> >> DLS >> >> HEALTH INDUSTRY >> April 9, 2012, 7:22 p.m. ET >> Prostate-Test Fees Challenged >> >> By CHRISTOPHER WEAVER >> Doctors in urology groups that profit from tests for prostate cancer order more of them than doctors who send samples to independent laboratories, according to a study Monday in the journal Health Affairs. >> >> The study found that doctors' practices that do their own lab work bill the federal Medicare program for analyzing 72% more prostate tissue samples per biopsy while detecting fewer cases of cancer than counterparts who send specimens to outside labs. >> >> Hiring pathologists boosts revenue for a practice and creates a potential incentive to increase the number of tests ordered, said Jean Mitchell, a Georgetown University economist and author of the study. >> >> That fewer cancers were detected-21% versus 35% for those sent to external labs, according to the study-suggests "financial incentives" >> may play a role in decisions to order the tests, Ms. Mitchell said. >> >> Some urologists said the research doesn't necessarily indicate financial motives. Urologists in larger group practices that have in-house pathologists may be more aggressive in testing because they seek to catch cancer earlier, said Steven Schlossberg, a Yale urologist who heads a health-policy panel for the American Urological Association and wasn't involved in the research. Also, Dr. Schlossberg noted, the figures, which cover 36,261 biopsies from 2005 through 2007, are five years old. >> >> The study was financed by the College of American Pathologists and the American Clinical Laboratory Association. It is the last salvo in a turf war between laboratory companies and physician groups that have opened their own labs to conduct tests. >> >> Regulators and economists scrutinizing the growing costs of health care have targeted a range of related activities by doctors, known as self-referrals. >> >> Although a set of 1990s-era laws, named for their proponent, Rep. Pete Stark (D., Calif.), ban doctors from referring patients to most companies in which they have a financial interest, urology groups can enter the pathology business because of an exemption for certain services performed within physicians' offices. The pathologists and other groups are lobbying Congress to end the exemption. >> >> At issue in the study is a quirk of billing for lab procedures. Labs get paid based on the number of jars used to hold specimens from a prostate biopsy. Doctors can choose to put several specimens in one jar or put each in its own jar, potentially boosting lab fees, which averaged about $104 a jar in 2010, according to the study. >> >> Urologists in practices with in-house pathologists sent 11.4 jars per biopsy for testing versus 5.9 jars per biopsy for other doctors in 2005. >> >> >> Some doctors say that separating the samples can help them better map any cancer. >> >> In addition, urologists in recent years have been taking more samples during a biopsy to better identify the location of any cancer, said John Hollingsworth, an assistant professor of urology at the University of Michigan. The standard number of samples taken doubled to 12 over the last decade, he said. >> >> The Health Affairs study's conclusions are "largely around billing practices, not around clinical practices," said George Kwass, a pathologist based in Massachusetts and board member of the College of American Pathologists. Urologists who team up with pathologists appear to bill more, he said, leading to potential waste. >> >> Urology groups are consolidating, and increasingly moving into the pathology business. One large practice based on New York's Long Island, Integrated Medical Professionals, opened its lab in 2010 to control costs and because doctors encountered errors in outside test results, said the group's chairman, Deepak Kapoor. >> >> "We don't make a fortune on pathology," Dr. Kapoor said. >> >> But lab businesses are seeing revenue vanish. Texas pathology group ProPath stopped getting prostate tissue from large urology groups more than four years ago, said executive director Krista Crews, when these clients began doing lab work in-house. The group still gets referrals from small, one and two-doctor practices, she said. >> >> Large laboratory companies are worried about the trend, too. Quest Diagnostics Inc. DGX -2.52%said in its latest annual filings that if physicians, including urologists as well as gastroenterologists and skin and cancer doctors, continued to "internalize" testing services, it could reduce the company's sales. >> >> Write to Christopher Weaver at christopher.weaver@wsj.com >> >> Copyright 2012 Dow Jones& Company, Inc. All Rights Reserved >> >> This copy is for your personal, non-commercial use only. Distribution and use of this material are governed by our Subscriber Agreement and by copyright law. For non-personal use or to order multiple copies, please contact Dow Jones Reprints at 1-800-843-0008 or visit _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From boneimage8 <@t> gmail.com Wed Apr 11 09:33:55 2012 From: boneimage8 <@t> gmail.com (Marc DeCarlo) Date: Wed Apr 11 09:34:04 2012 Subject: [Histonet] New to paraffin cutting - seeking advice In-Reply-To: References: Message-ID: Merissa, I can't help with your paraffin needs, but have a suggestion for your plastic. Try Weigerts Hematoxylin. It's available commercially through several vendors and in my experience stains just like what your looking for. If you need help just contact me. Marc On Monday, April 9, 2012, M.O. wrote: > Hello everyone! I have had such wonderful feedback regarding plastic > embedded specimens and now I am moving onto paraffin. The majority of the > specimens I am going to be cutting are paraffin embedded decal rabbit > femurs. I am using a Leica RM2255 and there is an option for retraction > (after each section) and blade angle. I need to practice a lot because > right now my sections are crinkly even though I have my samples on ice, but > hopefully practice makes perfect! > > So I want to ask you all a few questions regarding settings and technique > and one about H&E staining. What angle should the blade be at - I have it > set at 0 for the moment. What about the retraction option - I have it set > at 15um. The sections are being cut at 4um. > > When the blade has pieces of paraffin on it, do I need to clean that off? > How do I remove this paraffin because I don't want to dull the blade? If > not, does that impact the section quality and should I be moving to a > different area of the blade? > > I am looking into flotation baths that are relatively inexpensive. In > particular, I am looking for a simple bath with a glass pyrex dish. Do you > have any suggestions on where to purchase one from? > > Lastly, I am going back to H&E staining of plastic embedded undecalcified > bone, specifically hematoxylin, for a brief moment. When I use a clearing > solution I know that I am trying to destain a bit from the undecalcified > bone to make a lighter stain, but does this also significantly destain the > nuclei? Right now, I need to leave the sample staining in Harris's > hematoxylin for more than 4 mins to get the nuclei stained nicely, but I > need to destain because the section is too dark. I just don't want to > destain the nuclei too much, but want a lighter stain on the undecal bone. > > Again, thank you all for your support and advice, it is much appreciated! > > Sincerely, > Merissa > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From indytreegers <@t> sbcglobal.net Wed Apr 11 09:50:32 2012 From: indytreegers <@t> sbcglobal.net (indytreegers@sbcglobal.net) Date: Wed Apr 11 09:50:37 2012 Subject: [Histonet] Slide disposal Message-ID: <1334155832.12840.YahooMailClassic@web81907.mail.mud.yahoo.com> Can anyone tell me the proper way to dispose of slides?? Are they considered biohazardous waste? ? Thanks so much, Lyn ? From nicole <@t> dlcjax.com Wed Apr 11 09:52:36 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed Apr 11 09:52:47 2012 Subject: [Histonet] In House Labs in WSJ In-Reply-To: <4F85938C.5090701@umdnj.edu> References: <8D7C2D242DBD45498006B21122072BF8A4F3E89C@MCINFRWEM003.ucsfmedicalcenter.org> <4ED53330-0BF5-4208-A1F5-81AB2D942FC4@yahoo.com> <4F85938C.5090701@umdnj.edu> Message-ID: <3759.208.62.167.196.1334155956.squirrel@webmail.realpages.com> Agreed , but the idea in recent health care has been early detection. So as technology increased, more diagnostic test were ordered. But, that is not only pathology, its micro, radiology, ultrasound, chemistry, etc. These early test did drive cost up, but also saved cost. Its kinda no win. If a radical tumor was detected early it could be removed by surgery and the patient could possibly still live a healthy cancer free life. If not detected, the patient could suffer through chemo and expensive radiation, and expensive hospice. Leaving the family devistated with medical bills and the loss of a loved one that a simple diagnostic test could have detected. Also, there is a huge problem with the malpractice suits in our country. If the pateint did suffer or die because a simple test was not ordered that could save their life the physician is held responsible and sued and could possibly lose his license and career. If less test are the answer to cutting cost to our rising health deficit, then doctors should be more protected when they make choices not to order tests that could save your life. Despite cost, in court they will argue it was a "simple" inexpensive test that could have saved his or her life. The physician is charged with protecting a patients health and he needs tool to do that. Tools that are being taken because they are unaffordable. We must learn how to manage our resources at every level. I for one would be devistated if I had cervical cancer because my OB did not submit a specimen when my pap came back as abnormal. I would be willing to pay the path fee out of pocket to have an answer. But, that's also part of the problem. People do not want to pay for services they recieve. But, they have a really nice flat screen and iphone. This economic crisis is a result of the public and health professional and gas prices, etc. We must stick together and come up with ways to still use diagnostic test effectively. They do save lives and save money, maybe on a small scale compared to those who are not diagnosed with any condition. Our current health care model has been based on detection and prevention. It will have to change for our industry to survive. Resouces will have to be rationed but I fear it is being given the title of over-utilization instead. As current tests decrease and physician are pushed to order less; I fear there will be an increase of misdiagnosises and an increase in malpractice suits. Its becomming scarry out there.. This change will effect each one of us. Nicole Tatum HT ASCP On 4/10/2012 5:33 PM, Kim Donadio wrote: >>> Less screening = fewer biopsies = less revenue = less prostate cancers >>> caught early = more deaths to prostate cancers. >> Would you not agree? > > No. There is very good scientific evidence that screening does not > increase survival rates but it does drive up costs and unnecessary > surgery and related complications. > I can send the papers from NEJM if you like. > > Geoff > >> >> And for all those advocating closure of private labs, do you also feel >> the same way about private pathologist owned labs who reep the benefits >> of getting all the out PT work from affiliated physicians while they >> also get a fee to serve as medical directors of hospital labs and get >> the pc portion of hospital work of which they can order as many test >> they want so they get the pc portion while the hospital gets the tc and >> all the big bills associated with doing the test making it hard on tax >> payer as well because so much in a hospital is already subsidize by the >> gov. >> >> Is what you really want is to have all pathologist as employees of the >> hospitals? And have the hospital bill global. >> >> And a few walmart like reference labs >> >> I'm just curious as to the exact position of some on here. >> >> Thanks >> >> Kim >> Sent from my iPhone >> >> On Apr 10, 2012, at 2:39 PM, "Morken, >> Timothy" wrote: >> >>> Not surprising since our health care system is biased to pay for tests >>> and treatments, not results. On top of this there are serious questions >>> as to whether the PSA screening that leads to biopsies is useful in the >>> long term. There is a recommendation out there to stop PSA screening >>> for most men since it is largely non-specific. That test is what leads >>> to the biopsies. Less screening = fewer biopsies = less revenue. >>> >>> Tim Morken >>> >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel >>> Schneider >>> Sent: Tuesday, April 10, 2012 11:22 AM >>> To: Histonet >>> Subject: [Histonet] In House Labs in WSJ >>> >>> The Wall Street Journal served up a timely article for us. >>> You'll see both sides of the argument below. One side is right. >>> >>> DLS >>> >>> HEALTH INDUSTRY >>> April 9, 2012, 7:22 p.m. ET >>> Prostate-Test Fees Challenged >>> >>> By CHRISTOPHER WEAVER >>> Doctors in urology groups that profit from tests for prostate cancer >>> order more of them than doctors who send samples to independent >>> laboratories, according to a study Monday in the journal Health >>> Affairs. >>> >>> The study found that doctors' practices that do their own lab work bill >>> the federal Medicare program for analyzing 72% more prostate tissue >>> samples per biopsy while detecting fewer cases of cancer than >>> counterparts who send specimens to outside labs. >>> >>> Hiring pathologists boosts revenue for a practice and creates a >>> potential incentive to increase the number of tests ordered, said Jean >>> Mitchell, a Georgetown University economist and author of the study. >>> >>> That fewer cancers were detected-21% versus 35% for those sent to >>> external labs, according to the study-suggests "financial incentives" >>> may play a role in decisions to order the tests, Ms. Mitchell said. >>> >>> Some urologists said the research doesn't necessarily indicate >>> financial motives. Urologists in larger group practices that have >>> in-house pathologists may be more aggressive in testing because they >>> seek to catch cancer earlier, said Steven Schlossberg, a Yale urologist >>> who heads a health-policy panel for the American Urological Association >>> and wasn't involved in the research. Also, Dr. Schlossberg noted, the >>> figures, which cover 36,261 biopsies from 2005 through 2007, are five >>> years old. >>> >>> The study was financed by the College of American Pathologists and the >>> American Clinical Laboratory Association. It is the last salvo in a >>> turf war between laboratory companies and physician groups that have >>> opened their own labs to conduct tests. >>> >>> Regulators and economists scrutinizing the growing costs of health care >>> have targeted a range of related activities by doctors, known as >>> self-referrals. >>> >>> Although a set of 1990s-era laws, named for their proponent, Rep. Pete >>> Stark (D., Calif.), ban doctors from referring patients to most >>> companies in which they have a financial interest, urology groups can >>> enter the pathology business because of an exemption for certain >>> services performed within physicians' offices. The pathologists and >>> other groups are lobbying Congress to end the exemption. >>> >>> At issue in the study is a quirk of billing for lab procedures. Labs >>> get paid based on the number of jars used to hold specimens from a >>> prostate biopsy. Doctors can choose to put several specimens in one jar >>> or put each in its own jar, potentially boosting lab fees, which >>> averaged about $104 a jar in 2010, according to the study. >>> >>> Urologists in practices with in-house pathologists sent 11.4 jars per >>> biopsy for testing versus 5.9 jars per biopsy for other doctors in >>> 2005. >>> >>> >>> Some doctors say that separating the samples can help them better map >>> any cancer. >>> >>> In addition, urologists in recent years have been taking more samples >>> during a biopsy to better identify the location of any cancer, said >>> John Hollingsworth, an assistant professor of urology at the University >>> of Michigan. The standard number of samples taken doubled to 12 over >>> the last decade, he said. >>> >>> The Health Affairs study's conclusions are "largely around billing >>> practices, not around clinical practices," said George Kwass, a >>> pathologist based in Massachusetts and board member of the College of >>> American Pathologists. Urologists who team up with pathologists appear >>> to bill more, he said, leading to potential waste. >>> >>> Urology groups are consolidating, and increasingly moving into the >>> pathology business. One large practice based on New York's Long Island, >>> Integrated Medical Professionals, opened its lab in 2010 to control >>> costs and because doctors encountered errors in outside test results, >>> said the group's chairman, Deepak Kapoor. >>> >>> "We don't make a fortune on pathology," Dr. Kapoor said. >>> >>> But lab businesses are seeing revenue vanish. Texas pathology group >>> ProPath stopped getting prostate tissue from large urology groups more >>> than four years ago, said executive director Krista Crews, when these >>> clients began doing lab work in-house. The group still gets referrals >>> from small, one and two-doctor practices, she said. >>> >>> Large laboratory companies are worried about the trend, too. Quest >>> Diagnostics Inc. DGX -2.52%said in its latest annual filings that if >>> physicians, including urologists as well as gastroenterologists and >>> skin and cancer doctors, continued to "internalize" testing services, >>> it could reduce the company's sales. >>> >>> Write to Christopher Weaver at christopher.weaver@wsj.com >>> >>> Copyright 2012 Dow Jones& Company, Inc. All Rights Reserved >>> >>> This copy is for your personal, non-commercial use only. Distribution >>> and use of this material are governed by our Subscriber Agreement and >>> by copyright law. For non-personal use or to order multiple copies, >>> please contact Dow Jones Reprints at 1-800-843-0008 or visit >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From nicole <@t> dlcjax.com Wed Apr 11 10:04:47 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed Apr 11 10:04:55 2012 Subject: [Histonet] Aetna in-house Message-ID: <3841.208.62.167.196.1334156687.squirrel@webmail.realpages.com> I have reviewed my letter from Aetna and it reads," This change is consistent with the Center for Medicare & Medicaid Services recongnition of CAP as an approved accreditation organization for non-hospital anatomic pathology testing." I have called Medicare and they state that they have made no changes and CLIA is the only enitity they require to recieve reimbursment for path services reguardless of location. If that was the case I would definitly have to get my CAP. Because if Medicare does, it you know they will all follow suit. So, to any smaller lab. Medicare has not changed to CAP and CLIA remains the only certification you need. Nicole Tatum HT ASCP From dunatrsd <@t> sbcglobal.net Wed Apr 11 10:11:39 2012 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Wed Apr 11 10:11:47 2012 Subject: [Histonet] CSH 2012 Symposium-San Diego CA In-Reply-To: <3841.208.62.167.196.1334156687.squirrel@webmail.realpages.com> References: <3841.208.62.167.196.1334156687.squirrel@webmail.realpages.com> Message-ID: <1334157099.57410.YahooMailRC@web83902.mail.sp1.yahoo.com> ?Come Ride the Waves of Innovation? ? This year?s meeting will be at the Bahia resort hotel on Mission Bay.? 2 Blocks from the Ocean.? We are Presently lining up an outstanding list of lecturers.? Classes will be held on 2 paddle wheel boats.? Take a look at the hotel website and start planning now.? Details and registration will be posted soon on the Society website. ? http://www.bahiahotel.com/ http://www.californiahistology.org/events.html ? The 2012 Symposium/Convention will be held in May in San Diego, CA. When: May 3 - 6, 2012 Hotel Information: Bahia Resort Hotel 998 West Mission Bay Drive, San Diego CA 858.488.0551 Reserve your room now at: https://shop.evanshotels.com/bahia_groups/casocf1205093.html ? Note: To receive the group rate of $109.00 per night please mention CSH when registering with the hotel. The cutoff date for the group rate is April 3, 2012.? We have already met our room number minimum, but more rooms are available at the discount rate.? Register and book you room soon. ? We still have a few vendor tables available, but they are filling fast. ? ? James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org From Wanda.Smith <@t> HCAhealthcare.com Wed Apr 11 11:45:43 2012 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Wed Apr 11 11:45:52 2012 Subject: [Histonet] Slide disposal In-Reply-To: <1334155832.12840.YahooMailClassic@web81907.mail.mud.yahoo.com> References: <1334155832.12840.YahooMailClassic@web81907.mail.mud.yahoo.com> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA273D100F6A@NADCWPMSGCMS03.hca.corpad.net> We package ours up to be incinerated. Not so much because of biohazards, but our patient's names are on all the slides. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of indytreegers@sbcglobal.net Sent: Wednesday, April 11, 2012 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide disposal Can anyone tell me the proper way to dispose of slides?? Are they considered biohazardous waste? ? Thanks so much, Lyn ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> uropartners.com Wed Apr 11 15:07:20 2012 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Wed Apr 11 15:07:25 2012 Subject: [Histonet] HIPAA audit Message-ID: Hello All: Do any of you have experience with internal HIPAA audits or monitoring, as required by CAP checklist question Gen.41303? We have a HIPAA policy but have never done an audit. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 From tilycyn <@t> auburn.edu Wed Apr 11 15:10:01 2012 From: tilycyn <@t> auburn.edu (Cynthia Hutchinson) Date: Wed Apr 11 15:10:13 2012 Subject: [Histonet] Looking for protzoal antibodies Message-ID: <2872A2FD0D55EF4EA941EF72CC1ED6250809895F@exmb3.auburn.edu> Hello All, Does anyone know of a source for clean Neospora caninum and/or Sarcocystis antibodies? Also, does anyone have any positive control tissue for toxo, neo, and/or sarco? Thank you, Cindy Cynthia Tily Hutchinson Rsch Asst IV Pathobiology Coll of Vet Med Auburn University Ph: 334-844-7020 Fax: 334-844-2652 From shive003 <@t> umn.edu Wed Apr 11 15:15:37 2012 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Apr 11 15:15:43 2012 Subject: [Histonet] Looking for protzoal antibodies In-Reply-To: <2872A2FD0D55EF4EA941EF72CC1ED6250809895F@exmb3.auburn.edu> References: <2872A2FD0D55EF4EA941EF72CC1ED6250809895F@exmb3.auburn.edu> Message-ID: I get my Neospora caninum from VMRD; it's a goat polyclonal, but you can dilute it way out so there's no background (currently using 1:24,000). I may have a Toxo positive block for you. Jan Shivers IHC/Histo/EM Section Head Veterinary Diagnostic Lab UMN College of Veterinary Medicine St. Paul, MN On Wed, Apr 11, 2012 at 3:10 PM, Cynthia Hutchinson wrote: > Hello All, > > Does anyone know of a source for clean Neospora caninum and/or Sarcocystis > antibodies? > Also, does anyone have any positive control tissue for toxo, neo, and/or > sarco? > > Thank you, > Cindy > > Cynthia Tily Hutchinson > Rsch Asst IV > Pathobiology > Coll of Vet Med > Auburn University > Ph: 334-844-7020 > Fax: 334-844-2652 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From fbozkurt <@t> gmail.com Wed Apr 11 15:16:24 2012 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Wed Apr 11 15:16:27 2012 Subject: [Histonet] Looking for protzoal antibodies In-Reply-To: <2872A2FD0D55EF4EA941EF72CC1ED6250809895F@exmb3.auburn.edu> References: <2872A2FD0D55EF4EA941EF72CC1ED6250809895F@exmb3.auburn.edu> Message-ID: Hello, there is antibody of neospora caninum in VMRD http://www.vmrd.com/ .. I didnt use but i have heard that is good working. for sarcocyst I can send blocks by regular mail. But Imho no need IHC for sarcocyst. On Wed, Apr 11, 2012 at 11:10 PM, Cynthia Hutchinson wrote: > Hello All, > > Does anyone know of a source for clean Neospora caninum and/or Sarcocystis > antibodies? > Also, does anyone have any positive control tissue for toxo, neo, and/or > sarco? > > Thank you, > Cindy > > Cynthia Tily Hutchinson > Rsch Asst IV > Pathobiology > Coll of Vet Med > Auburn University > Ph: 334-844-7020 > Fax: 334-844-2652 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 From twheelock <@t> mclean.harvard.edu Wed Apr 11 15:42:39 2012 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Wed Apr 11 15:42:43 2012 Subject: [Histonet] Thank you Message-ID: <4F85ECBF.3010403@mclean.harvard.edu> Hi Everyone: I want to thank everyone who gave me advice concerning my cassette labeling problem. I am trying out three different types of markers pens: StatLab, Leica, and NewComer Supply Histo-tec brands. We will also make sure to let the ink dry before putting the cassettes into formalin. If we still have a problem, we will experiment with a different cassette. Thanks again. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 From rat1 <@t> rice.edu Wed Apr 11 16:41:07 2012 From: rat1 <@t> rice.edu (rat1@rice.edu) Date: Wed Apr 11 16:41:10 2012 Subject: [Histonet] Sections of poly(caprolactone) based scaffolds washing off the slide Message-ID: <20120411164107.188525tg5f7uq5wz@webmail.rice.edu> Dear Histonet, Sorry in advance for the long email. I would like to ask for advice regarding some of my sections rinsing off my slides. I generate composite tissue engineered electrospun poly(caprolactone) (PCL) and cell-generated extracellular matrix (ECM) scaffolds. With larger amount of ECM coated around the PCL, I have no issues of retaining the entire section. However, with minimal ECM coating, the PCL portion of the section is easily washed off. The PCL portion of the scaffold is a porous electrospun random fiber mesh with fiber diameters of 10 microns. The method I?ve tried is detailed below. 1. Fix the composite scaffold in 2.5% glutaraldehyde for 45 minutes. 2. Soak in Histoprep overnight at 4 C to ensure distribution of the Histoprep throughout the pores of the scaffold. 3. Embed the scaffold and freeze into blocks. 4. Let the embedded scaffold sit in the -80 C freezer overnight then transfer to the -20 C freezer. (this helps with the sectioning of our scaffolds, better integration of the scaffold with the rest of the Histoprep) 5. Cryosection into 5 micron sections and place onto superfrost plus slides. After this I run into problems. I?ve kept the slides at -20 C and let them warm to room temp before staining with picrosirus red or Safranin O etc. as well as baking them on the slide warmer at 45 C for 1 day and up to a week. I?ve also tried using superfrost excell slides, poly(L-lysine) coated slides, unaltered glass slides, and silanized slides (I didn?t expect it to work since the embedding medium is hydrophilic and rightly so, the entire section just beaded up destroying the section). Each of these processes resulted in the PCL portion of the scaffold rinsing off within 2 or 3 steps of the stain except for the 7 day baking step, which retained parts but not all of the PCL portion. For the staining procedure, I outline the section with a Dako pen, then drip the water, stains, etc. into the outline but not on the scaffold itself using a transfer pipet until everything is covered. To remove the liquid, I use a pipettor set to 200 microliters to aspirate, again away from the section/scaffold, but since the PCL is adherent to the slide, it gets aspirated off along with the water. Using an autostainer is way to harsh for these scaffolds, so I reverted to careful manual staining. Additionally, due to the properties of the PCL, I can?t embed using paraffin since the melting point of PCL is 60 C and PCL dissolves in xylene, chloroform, acetone, DMF, and THF. I also can?t use gelatin coated slides since I need to stain for the collagen in the ECM coating. Does anyone have any insight to help retain the PCL portion of the scaffolds? Steps I could take, different slides that may work better? I do know that PCL becomes hydrophilic if treated with a high normal NaOH (i.e. the ester bonds in the polymer breaks down to carboxylic groups), but I?m not sure if that could work, since if I perform the NaOH treatment before sectioning only the outside of the fibers will become hydrophilic and if I do it after sectioning, it wouldn?t affect the bottom portion of the section. This could also damage the ECM as well. I can upload pictures of before and after staining if it would help. I would appreciate any help you could suggest. Thanks, Rich From rat1 <@t> rice.edu Wed Apr 11 16:57:04 2012 From: rat1 <@t> rice.edu (Richard Thibault) Date: Wed Apr 11 16:57:39 2012 Subject: [Histonet] Sections of poly(caprolactone) based scaffolds washing off the slide In-Reply-To: <20120411164107.188525tg5f7uq5wz@webmail.rice.edu> References: <20120411164107.188525tg5f7uq5wz@webmail.rice.edu> Message-ID: Sorry, for clarification I meant the PCL isn't* adherent to the slide and way too* harsh On Wed, Apr 11, 2012 at 16:41, wrote: > Dear Histonet, > > Sorry in advance for the long email. I would like to ask for advice > regarding some of my sections rinsing off my slides. I generate composite > tissue engineered electrospun poly(caprolactone) (PCL) and cell-generated > extracellular matrix (ECM) scaffolds. With larger amount of ECM coated > around the PCL, I have no issues of retaining the entire section. However, > with minimal ECM coating, the PCL portion of the section is easily washed > off. The PCL portion of the scaffold is a porous electrospun random fiber > mesh with fiber diameters of 10 microns. The method I?ve tried is detailed > below. > > 1. Fix the composite scaffold in 2.5% glutaraldehyde for 45 minutes. > 2. Soak in Histoprep overnight at 4 C to ensure distribution of the > Histoprep throughout the pores of the scaffold. > 3. Embed the scaffold and freeze into blocks. > 4. Let the embedded scaffold sit in the -80 C freezer overnight then > transfer to the -20 C freezer. (this helps with the sectioning of our > scaffolds, better integration of the scaffold with the rest of the > Histoprep) > 5. Cryosection into 5 micron sections and place onto superfrost plus > slides. > > After this I run into problems. I?ve kept the slides at -20 C and let > them warm to room temp before staining with picrosirus red or Safranin O > etc. as well as baking them on the slide warmer at 45 C for 1 day and up to > a week. I?ve also tried using superfrost excell slides, poly(L-lysine) > coated slides, unaltered glass slides, and silanized slides (I didn?t > expect it to work since the embedding medium is hydrophilic and rightly so, > the entire section just beaded up destroying the section). Each of these > processes resulted in the PCL portion of the scaffold rinsing off within 2 > or 3 steps of the stain except for the 7 day baking step, which retained > parts but not all of the PCL portion. > > For the staining procedure, I outline the section with a Dako pen, then > drip the water, stains, etc. into the outline but not on the scaffold > itself using a transfer pipet until everything is covered. To remove the > liquid, I use a pipettor set to 200 microliters to aspirate, again away > from the section/scaffold, but since the PCL is adherent to the slide, it > gets aspirated off along with the water. Using an autostainer is way to > harsh for these scaffolds, so I reverted to careful manual staining. > > Additionally, due to the properties of the PCL, I can?t embed using > paraffin since the melting point of PCL is 60 C and PCL dissolves in > xylene, chloroform, acetone, DMF, and THF. I also can?t use gelatin coated > slides since I need to stain for the collagen in the ECM coating. > > Does anyone have any insight to help retain the PCL portion of the > scaffolds? Steps I could take, different slides that may work better? I > do know that PCL becomes hydrophilic if treated with a high normal NaOH > (i.e. the ester bonds in the polymer breaks down to carboxylic groups), but > I?m not sure if that could work, since if I perform the NaOH treatment > before sectioning only the outside of the fibers will become hydrophilic > and if I do it after sectioning, it wouldn?t affect the bottom portion of > the section. This could also damage the ECM as well. I can upload > pictures of before and after staining if it would help. I would appreciate > any help you could suggest. > > Thanks, > > Rich > > > > ______________________________**_________________ > Histonet mailing list > Histonet@lists.utsouthwestern.**edu > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet > > From pruegg <@t> ihctech.net Wed Apr 11 17:05:04 2012 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Apr 11 17:05:09 2012 Subject: [Histonet] Thank you In-Reply-To: <4F85ECBF.3010403@mclean.harvard.edu> References: <4F85ECBF.3010403@mclean.harvard.edu> Message-ID: <2069507642EE439D8656568AD076DA07@prueggihctechlt> The best markers I have ever used are called KP Markers, they were off the market for a while, but they are back and we get them from Mercedes Medical, just got a new batch and they are wonderful just like the old KP markers, we won't have anything else in the lab. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Wednesday, April 11, 2012 2:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thank you Hi Everyone: I want to thank everyone who gave me advice concerning my cassette labeling problem. I am trying out three different types of markers pens: StatLab, Leica, and NewComer Supply Histo-tec brands. We will also make sure to let the ink dry before putting the cassettes into formalin. If we still have a problem, we will experiment with a different cassette. Thanks again. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ladams <@t> cvpath.org Wed Apr 11 18:05:52 2012 From: ladams <@t> cvpath.org (Lila Adams) Date: Wed Apr 11 18:05:57 2012 Subject: [Histonet] 2012 Region II Symposium 26-28 Apr.2012, Williamsburg, VA Message-ID: <000601cd1837$a4b3b100$ee1b1300$@org> Hello to Histonetters , Just wanted to let you know there's a Region II Symposium on 26-28 April 2012 in Williamsburg, Virginia. There will be a choice of 18 to 19 workshops/lectures being offered during the three days. Fees are $50.00 for Thursday, $100.00 for Friday, $80.00 for Saturday or $200.00 for all three days. CEU's will be offered for most workshops and lectures. Go to the nsh.org/calendar and click on Region II Symposium for the entire brochure. If you have questions concerning registration contact Michelle Hart 302-733-4709 or Elaine Perkins 302-733-3659. The symposium fees include continental breakfast, breaks, lunch and a reception on Friday 27th April 2012. Come join in the Fun! Lila R. Adams IHC Research Lab CVPath Institute, Inc 19 Firstfield Road Gaithersburg, MD 20878 301-208-3570 ext 138 From ladams <@t> cvpath.org Wed Apr 11 18:15:55 2012 From: ladams <@t> cvpath.org (Lila Adams) Date: Wed Apr 11 18:15:59 2012 Subject: [Histonet] Job opening Gaithersburg,MD Message-ID: <000b01cd1839$0c19eac0$244dc040$@org> Anyone interested in Histology job? CV Path Institute, Inc is a laboratory based in Gaithersburg, MD focused primarily on cardiovascular medical research and pre-clinical studies seeks a full-time Histotechnician. Candidate must have expertise in embedding, microtomy, routine and special staining In paraffin sections. HT (ASCP) certification or HT eligible is preferred. Medical insurance coverage and retirement package include as benefit. Please send resume/CV with salary requirements to dhowd@cvpath.org . Lila R. Adams IHC Research Lab CVPath Institute, Inc 19 Firstfield Road Gaithersburg, MD 20878 301-208-3570 ext 138 From louise.renton <@t> gmail.com Thu Apr 12 02:10:34 2012 From: louise.renton <@t> gmail.com (Louise Renton) Date: Thu Apr 12 02:10:43 2012 Subject: [Histonet] Thank you In-Reply-To: <2069507642EE439D8656568AD076DA07@prueggihctechlt> References: <4F85ECBF.3010403@mclean.harvard.edu> <2069507642EE439D8656568AD076DA07@prueggihctechlt> Message-ID: there's always good old pencil (HB) as a backup On Thu, Apr 12, 2012 at 12:05 AM, Patsy Ruegg wrote: > The best markers I have ever used are called KP Markers, they were off the > market for a while, but they are back and we get them from Mercedes > Medical, > just got a new batch and they are wonderful just like the old KP markers, > we > won't have anything else in the lab. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim > Wheelock > Sent: Wednesday, April 11, 2012 2:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Thank you > > Hi Everyone: > > I want to thank everyone who gave me advice concerning my cassette > labeling problem. > I am trying out three different types of markers pens: StatLab, Leica, > and NewComer Supply Histo-tec brands. > We will also make sure to let the ink dry before putting the cassettes > into formalin. > If we still have a problem, we will experiment with a different cassette. > Thanks again. > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > 617-855-3592 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From jp1000r <@t> hotmail.com Thu Apr 12 06:21:12 2012 From: jp1000r <@t> hotmail.com (Jean-Philippe REY) Date: Thu Apr 12 06:21:24 2012 Subject: [Histonet] (no subject) Message-ID: You can work today comfortabIe from your home http://centroorientamentodonbosco.it/pebare.php?acarank=938 Thu, 12 Apr 2012 12:21:12 ______________ " I wished I was out of that tree, but I dasntcome down." (c) romeo aldon From Jonathan.Cremer <@t> med.kuleuven.be Thu Apr 12 07:14:09 2012 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Thu Apr 12 07:14:22 2012 Subject: [Histonet] Powdered egg white for endogenous biotin blocking Message-ID: Does anyone know if the powdered egg white from Sigma (E0500) still contains active streptavidin? I would like to use it to block endogenous biotin instead of going through the hassle of obtaining fresh eggs, but I've read that avidin is inactivated by heat (pasteurization, in this case). Also, if anyone already uses powdered egg white for this purpose, what concentration? Will 5% do? Many thanks, Regards, Jonathan --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven From trathborne <@t> somerset-healthcare.com Thu Apr 12 08:04:32 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Apr 12 08:04:00 2012 Subject: [Histonet] Thank you In-Reply-To: References: <4F85ECBF.3010403@mclean.harvard.edu> <2069507642EE439D8656568AD076DA07@prueggihctechlt> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711FBC772@SMCMAIL01.somerset-healthcare.com> Not necessarily true. We used pencils for years and are now trying different markers because the pencil is smudging. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louise Renton Sent: Thursday, April 12, 2012 3:11 AM To: Histonet Subject: Re: [Histonet] Thank you there's always good old pencil (HB) as a backup On Thu, Apr 12, 2012 at 12:05 AM, Patsy Ruegg wrote: > The best markers I have ever used are called KP Markers, they were off > the market for a while, but they are back and we get them from > Mercedes Medical, just got a new batch and they are wonderful just > like the old KP markers, we won't have anything else in the lab. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim > Wheelock > Sent: Wednesday, April 11, 2012 2:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Thank you > > Hi Everyone: > > I want to thank everyone who gave me advice concerning my cassette > labeling problem. > I am trying out three different types of markers pens: StatLab, Leica, > and NewComer Supply Histo-tec brands. > We will also make sure to let the ink dry before putting the cassettes > into formalin. > If we still have a problem, we will experiment with a different cassette. > Thanks again. > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > 617-855-3592 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From DKBoyd <@t> chs.net Thu Apr 12 08:44:04 2012 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Thu Apr 12 08:44:15 2012 Subject: [Histonet] Thank you In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711FBC772@SMCMAIL01.somerset-healthcare.com> References: <4F85ECBF.3010403@mclean.harvard.edu> <2069507642EE439D8656568AD076DA07@prueggihctechlt> <3AD061FE740D464FAC7BF6B5CFB7570711FBC772@SMCMAIL01.somerset-healthcare.com> Message-ID: <7EAFE982E328304DA6CE2B677BB762460BAD08D5@TN001WEXMBX12.US.chs.net> We have had the same problems as everyone else, i.e. pencil or markers on cassettes. What I am seeing is that the cassettes have changed. They are not textured on the writing edge as before. The edge is smooth and the pencil smudges. Some markers wash off. The problem here is alcohol, not xylene (substitutes) or formalin. Also KP Markers did have a bad batch that washed off as well. But as someone else commented, that too has been fixed. We use the KP Markers and have had no problems. There also was a batch of Fisher cassettes that where so smooth the ink/pencil faded to almost non-existent. We test every new marker before it is put into use in alcohol. If it bleeds we rub it to see if the numbers are still legible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, April 12, 2012 9:05 AM To: 'Louise Renton'; Histonet Subject: RE: [Histonet] Thank you Not necessarily true. We used pencils for years and are now trying different markers because the pencil is smudging. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louise Renton Sent: Thursday, April 12, 2012 3:11 AM To: Histonet Subject: Re: [Histonet] Thank you there's always good old pencil (HB) as a backup On Thu, Apr 12, 2012 at 12:05 AM, Patsy Ruegg wrote: > The best markers I have ever used are called KP Markers, they were off > the market for a while, but they are back and we get them from > Mercedes Medical, just got a new batch and they are wonderful just > like the old KP markers, we won't have anything else in the lab. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim > Wheelock > Sent: Wednesday, April 11, 2012 2:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Thank you > > Hi Everyone: > > I want to thank everyone who gave me advice concerning my cassette > labeling problem. > I am trying out three different types of markers pens: StatLab, Leica, > and NewComer Supply Histo-tec brands. > We will also make sure to let the ink dry before putting the cassettes > into formalin. > If we still have a problem, we will experiment with a different cassette. > Thanks again. > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > 617-855-3592 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From trathborne <@t> somerset-healthcare.com Thu Apr 12 09:50:16 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Apr 12 09:49:49 2012 Subject: [Histonet] New Reagent Lot Verification Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711FBC83F@SMCMAIL01.somerset-healthcare.com> ANP.22760 refers to new lot verification for antibodies and detection. The Evidence of Compliance says that there should be "Records of verification of new reagent lots". What is everyone's interpretation of "records"? Are you saving slides in addition to paper documentation? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Apr 12 09:54:01 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Apr 12 09:56:53 2012 Subject: [Histonet] RE: New Reagent Lot Verification In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711FBC83F@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570711FBC83F@SMCMAIL01.somerset-healthcare.com> Message-ID: My interpretation is that you have to compare the new lot to the previous. So I save the slides, so I have the previous to compare it to. But after that lot comparison, I don't know if you should save the slides or not. I'd like to know for sure, since I will surely run out of storage space soon. I save the paperwork for the required time as well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni [trathborne@somerset-healthcare.com] Sent: Thursday, April 12, 2012 10:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Reagent Lot Verification ANP.22760 refers to new lot verification for antibodies and detection. The Evidence of Compliance says that there should be "Records of verification of new reagent lots". What is everyone's interpretation of "records"? Are you saving slides in addition to paper documentation? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Thu Apr 12 10:00:43 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Apr 12 10:00:56 2012 Subject: [Histonet] Thank you In-Reply-To: <7EAFE982E328304DA6CE2B677BB762460BAD08D5@TN001WEXMBX12.US.chs.net> References: <4F85ECBF.3010403@mclean.harvard.edu> <2069507642EE439D8656568AD076DA07@prueggihctechlt> <3AD061FE740D464FAC7BF6B5CFB7570711FBC772@SMCMAIL01.somerset-healthcare.com> <7EAFE982E328304DA6CE2B677BB762460BAD08D5@TN001WEXMBX12.US.chs.net> Message-ID: We used Fisher Scientific cassettes for years without a problem. Then we had a big problem; smudged ink and failure to close. The writing surface was slick, not textured, there was not enough plastic injected into the mold to form the tabs on the locking lid and a plastic bleed between the locking tab and the cassette body prevented full closure of the cassette. I sent some samples to Fisher because they were interested in "seeing the problem". I didn't hear boo back. I switched suppliers. I'm happy again. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Thursday, April 12, 2012 7:44 AM To: Rathborne, Toni; 'Louise Renton'; Histonet Subject: RE: [Histonet] Thank you We have had the same problems as everyone else, i.e. pencil or markers on cassettes. What I am seeing is that the cassettes have changed. They are not textured on the writing edge as before. The edge is smooth and the pencil smudges. Some markers wash off. The problem here is alcohol, not xylene (substitutes) or formalin. Also KP Markers did have a bad batch that washed off as well. But as someone else commented, that too has been fixed. We use the KP Markers and have had no problems. There also was a batch of Fisher cassettes that where so smooth the ink/pencil faded to almost non-existent. We test every new marker before it is put into use in alcohol. If it bleeds we rub it to see if the numbers are still legible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, April 12, 2012 9:05 AM To: 'Louise Renton'; Histonet Subject: RE: [Histonet] Thank you Not necessarily true. We used pencils for years and are now trying different markers because the pencil is smudging. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louise Renton Sent: Thursday, April 12, 2012 3:11 AM To: Histonet Subject: Re: [Histonet] Thank you there's always good old pencil (HB) as a backup On Thu, Apr 12, 2012 at 12:05 AM, Patsy Ruegg wrote: > The best markers I have ever used are called KP Markers, they were off > the market for a while, but they are back and we get them from > Mercedes Medical, just got a new batch and they are wonderful just > like the old KP markers, we won't have anything else in the lab. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim > Wheelock > Sent: Wednesday, April 11, 2012 2:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Thank you > > Hi Everyone: > > I want to thank everyone who gave me advice concerning my cassette > labeling problem. > I am trying out three different types of markers pens: StatLab, Leica, > and NewComer Supply Histo-tec brands. > We will also make sure to let the ink dry before putting the cassettes > into formalin. > If we still have a problem, we will experiment with a different cassette. > Thanks again. > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > 617-855-3592 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu Apr 12 10:25:52 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Apr 12 10:26:01 2012 Subject: [Histonet] New Reagent Lot Verification In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711FBC83F@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570711FBC83F@SMCMAIL01.somerset-healthcare.com> Message-ID: We're saving both for at least 2 years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, April 12, 2012 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Reagent Lot Verification ANP.22760 refers to new lot verification for antibodies and detection. The Evidence of Compliance says that there should be "Records of verification of new reagent lots". What is everyone's interpretation of "records"? Are you saving slides in addition to paper documentation? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSiena <@t> statlab.com Thu Apr 12 10:33:03 2012 From: DSiena <@t> statlab.com (Debra Siena) Date: Thu Apr 12 10:33:12 2012 Subject: [Histonet] RE: New Reagent Lot Verification In-Reply-To: Message-ID: I have heard that you should keep the slides for 2 years or longer if your state requires it. Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsiena@statlab.com | www.statlab.com ----- Original Message ----- From: McMahon, Loralee A [mailto:Loralee_Mcmahon@URMC.Rochester.edu] Sent: Thursday, April 12, 2012 09:54 AM To: Rathborne, Toni ; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Reagent Lot Verification My interpretation is that you have to compare the new lot to the previous. So I save the slides, so I have the previous to compare it to. But after that lot comparison, I don't know if you should save the slides or not. I'd like to know for sure, since I will surely run out of storage space soon. I save the paperwork for the required time as well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni [trathborne@somerset-healthcare.com] Sent: Thursday, April 12, 2012 10:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Reagent Lot Verification ANP.22760 refers to new lot verification for antibodies and detection. The Evidence of Compliance says that there should be "Records of verification of new reagent lots". What is everyone's interpretation of "records"? Are you saving slides in addition to paper documentation? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Thu Apr 12 11:17:40 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Apr 12 11:17:58 2012 Subject: [Histonet] New Reagent Lot Verification In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711FBC83F@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB7570711FBC83F@SMCMAIL01.somerset-healthcare.com> Message-ID: Yes Sent from my iPhone On Apr 12, 2012, at 10:50 AM, "Rathborne, Toni" wrote: > ANP.22760 refers to new lot verification for antibodies and detection. The Evidence of Compliance says that there should be "Records of verification of new reagent lots". What is everyone's interpretation of "records"? Are you saving slides in addition to paper documentation? > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicole <@t> dlcjax.com Thu Apr 12 12:32:30 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Thu Apr 12 12:32:38 2012 Subject: [Histonet] ACMS perspective on Aetna's new requirments. Message-ID: <2628.208.62.167.196.1334251950.squirrel@webmail.realpages.com> This is a letter From the American College of Mohs Surgery in reguards to Aetna letter for CAP requiremnet. April 5, 2012 I read your letter of March 23, 2012, outlining Aetna?s new requirement for CLIA and CAP certification for certain pathology services with dismay. I am very concerned these requirements could lead to lower cure rates, increased tissue loss and scarring, and even unnecessary deaths in your patients with problem skin cancer with Aetna insurance. CAP certification will be almost impossible to achieve for small Mohs Micrographic surgery frozen section laboratories. These labs can only be CAP certified if directed by an anatomic pathologist or dermatopathologist. Mohs surgeons, who have extensive additional training in pathology, and training to run their laboratories, do not fit into either niche. Mohs surgeons deal with the most difficult and recurrent skin cancers. Please find attached a copy of the CPT coding assistant from 2008 describing Mohs surgery, and detailing what services are included in it. With your new restrictions, immunohistochemistry (CPT code 88342) cannot be billed with Mohs surgery for malignant melanoma and spindle cell tumors. This will restrict the use of Mart 1 immuno stains and other special cytokeratin stains. This will result in lower cure rates and an increased number of deaths. These stains on additional frozen sections are not part of Mohs surgery, and are appropriately billed for separately. In addition, Mohs surgeons will not be able to bill for the occasional special stains on frozen sections (CPT code 8814) such as oil red O on a sebaceous carcinoma, or toluidine blue (in addition to the hematoxylin and Eosin stained frozen sections) to clarify an area of inflammation. Performing these special stains on ambiguous frozen sections often saves the patient additional surgery and tissue loss, and saves Aetna money, because another stage of Mohs surgery is avoided. These additional stains are not part of the Mohs surgery and are appropriately billed for separately. Denial of these CPT codes may result in more tissue being removed unnecessarily, lower cure rates, higher recurrence rates, and potentially deaths. Quality control of the frozen section laboratory is crucial, and mandatory for CAP lab approval. This quality control involves processing of ?confirming? formalin sections off the frozen blocks of tissue is commonly performed in Mohs surgery laboratories for quality control and confirmation. This will no longer be a billable service (CPT code 88305) per your letter. This directly contradicts CAP own recommendations for the follow up processing of frozen sections (see attached). These confirming formalin sections are not part of Mohs surgery and are separately billable. Your decision not to cover code CPT 88305 makes it impossible, or at a minimum, fiscally prohibitive, to maintain quality control and to even consider complying with the new CAP accreditation you are demanding in the same letter. >From the perspective of the American College of Mohs surgery, our patients could continue to receive the best quality of care, and the overall cost to Aetna may actually be lower (by avoiding additional stages of Mohs surgery), if CPT Codes 88314, 88305, and 88342 were exempted for providers who also bill the Mohs surgery CPT codes 17311 or 17313. Sincerely Brett Coldiron, M.D., F.A.C.P. President American College of Mohs Surgery From jkrupp <@t> deltacollege.edu Thu Apr 12 14:48:34 2012 From: jkrupp <@t> deltacollege.edu (Jon Krupp) Date: Thu Apr 12 14:48:41 2012 Subject: [Histonet] Prepared STD slides? Message-ID: Hi I not a histo tech, nor do I play one on TV, so I might not be up to speed on this. A microbiology instructor asked me to see if there is a source of prepared slides of STD's that he could use in class. Maybe you know some place we could check? I have done some WWW searching, but have had a hard time finding prepared slides students could use with a microscope in class. Most everything seems to have gone digital and PowerPoint-ish. Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA 95207 209-954-5284 jkrupp@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College From histotalk <@t> yahoo.com Thu Apr 12 15:32:45 2012 From: histotalk <@t> yahoo.com (David Kemler) Date: Thu Apr 12 15:32:53 2012 Subject: [Histonet] HistoTALK at the Region III Meeting Message-ID: <1334262765.35947.YahooMailNeo@web120602.mail.ne1.yahoo.com> Hi HistoNetters - Tomorrow the HistoTALK "mobile" studio will be arriving late in the day?for the Region III Meeting at?Callaway Gardens, in Pine Mountain, GA,?We'll be there?to interview (it is not LIVE)?everyone and anyone daring to go before the HistoTALK microphone to be a guest on a later broadcast show. Even if you don't care to be a guest on the show, plan on stopping by on Saturday to say hi. ? There are plenty of open interview?times available on Saturday, so don't be shy! ? See you at the Region III Meeting which is hosted by my Georgia Society for Histotechnology friends. ? Yours, David? From sadey <@t> hotmail.ca Thu Apr 12 15:50:46 2012 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Thu Apr 12 15:50:49 2012 Subject: [Histonet] Competency assesment forms for Pathologists Message-ID: Hello netters:Does anyone have an example of a competency assessment form designed for pathologists?Thanks in advance:)Sheila From one_angel_secret <@t> yahoo.com Thu Apr 12 20:03:30 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Apr 12 20:03:37 2012 Subject: [Histonet] Prepared STD slides? In-Reply-To: References: Message-ID: <6E088F6E-6D78-43DD-A3A3-D9F2772C80E3@yahoo.com> Contact your local college Might help Sent from my iPhone On Apr 12, 2012, at 3:48 PM, Jon Krupp wrote: > Hi > > I not a histo tech, nor do I play one on TV, so I might not be up to speed on this. > > A microbiology instructor asked me to see if there is a source of prepared slides of STD's that he could use in class. > > Maybe you know some place we could check? I have done some WWW searching, but have had a hard time finding prepared slides students could use with a microscope in class. Most everything seems to have gone digital and PowerPoint-ish. > > Thanks > > Jon > > Jonathan Krupp > Delta College > 5151 Pacific Ave. > Box 212 > Stockton, CA 95207 > 209-954-5284 > jkrupp@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Fri Apr 13 04:03:33 2012 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Apr 13 04:09:39 2012 Subject: [Histonet] Prepared STD slides? In-Reply-To: <6E088F6E-6D78-43DD-A3A3-D9F2772C80E3@yahoo.com> References: <6E088F6E-6D78-43DD-A3A3-D9F2772C80E3@yahoo.com> Message-ID: <2DBC101318AF4479B45C1457B9AF4432@HP2010> A Google search of "prepared histology slides" found a company called Carolina http://www.carolina.com/ Click Life Sciences Click Microscopic slides Don't know if they have exactly what you want, but it's a start. Don't know anything about the company. Maybe someone on Histonet does. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 (Opinions expressed are mine, and do not reflect my place of employment.) -----Original Message----- From: Kim Donadio Sent: Thursday, April 12, 2012 9:03 PM To: Jon Krupp Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Prepared STD slides? Contact your local college Might help Sent from my iPhone On Apr 12, 2012, at 3:48 PM, Jon Krupp wrote: > Hi > > I not a histo tech, nor do I play one on TV, so I might not be up to speed > on this. > > A microbiology instructor asked me to see if there is a source of prepared > slides of STD's that he could use in class. > > Maybe you know some place we could check? I have done some WWW searching, > but have had a hard time finding prepared slides students could use with a > microscope in class. Most everything seems to have gone digital and > PowerPoint-ish. > > Thanks > > Jon > > Jonathan Krupp > Delta College > 5151 Pacific Ave. > Box 212 > Stockton, CA 95207 > 209-954-5284 > jkrupp@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Fri Apr 13 08:20:32 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Apr 13 08:20:59 2012 Subject: [Histonet] "know error system" - prostate biopsies Message-ID: <4F87EFE0.7400.0077.1@harthosp.org> I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From LRaff <@t> uropartners.com Fri Apr 13 08:24:43 2012 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Fri Apr 13 08:24:49 2012 Subject: [Histonet] "know error system" - prostate biopsies In-Reply-To: <4F87EFE0.7400.0077.1@harthosp.org> References: <4F87EFE0.7400.0077.1@harthosp.org> Message-ID: We do not! Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 8:21 AM To: Histonet Subject: [Histonet] "know error system" - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Fri Apr 13 08:26:46 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Apr 13 08:27:28 2012 Subject: [Histonet] Formalin asfety question In-Reply-To: <4F87EFE0.7400.0077.1@harthosp.org> References: <4F87EFE0.7400.0077.1@harthosp.org> Message-ID: <4940DF6D1C5FDF48931B6966AAEF939553201E@chimsx08.CHI.catholichealth.net> Is formalin an RCRA regulated waste? I am having some difficulty getting this answered and figured someone who is much brighter than myself out there may well know. If anyone has a reference, that would be a huge help William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 8:21 AM To: Histonet Subject: [Histonet] "know error system" - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From claycal44 <@t> yahoo.com Fri Apr 13 08:30:49 2012 From: claycal44 <@t> yahoo.com (nancy lowen) Date: Fri Apr 13 08:30:58 2012 Subject: [Histonet] Wrinkles in whole bone mouse tibias Message-ID: <1334323849.98400.YahooMailNeo@web164503.mail.gq1.yahoo.com> Hello to all, I am having on ongoing problem with wrinkles in the midshaft marrow area of sections of whole mouse tibias.? Leaving them in the waterbath longer does not help--water bath temp. is 48 to 49 degrees.? I have tried several waxes to help, and am currently using McCormick Paraplast Plus. Processing times are : 70%-1hour, 80% etoh,1hour, 95%-1hour, 3 100% alcohols at 1 hour each. 2 Citrisolvs at 1:30 hours each, and four waxes for 1hour, 1hour,1hour, and 3hours for the last. Specimens seem to cut fine, and the head of the bone is good, but the wrinkles are in the middle to lower part of the diaphysis. Is is possible that the polymers in the wax could be the problem? I see it mostly in the thinner, densely maarrow packed bones. If anyone has any ideas, could you please pass it along, as I am getting gray hairs from trying to figure it out. Also, I do not fix the bones myself, but most of the bones I get have been fixed for 24 hours in 10% Formalin. Thanks in advance. Nancy.lowen@Va.gov From NHeath <@t> Lifespan.org Fri Apr 13 08:41:01 2012 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Fri Apr 13 08:41:11 2012 Subject: [Histonet] "know error system" - prostate biopsies In-Reply-To: <4F87EFE0.7400.0077.1@harthosp.org> References: <4F87EFE0.7400.0077.1@harthosp.org> Message-ID: <130E8991F210424096EFC6F42EA33B2408D75B99@LSCOEXCH1.lsmaster.lifespan.org> When I worked per-diem for Plus Diagnostics here in Warwick RI we used it. It was a pain in the butt!! Nancy Heath, HT (ASCP) Neuropathology Technician Pathology Tech Specialist Dept. of Pathology., Div. of Neuropathology Rhode Island Hospital APC Blding, Flr 12, Rm 211 593 Eddy Street Providence, RI 02903 lab: 401-444-3246 fax: 401-444-8514 nheath@lifespan.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 9:21 AM To: Histonet Subject: [Histonet] "know error system" - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Fri Apr 13 09:12:11 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Apr 13 09:12:22 2012 Subject: [Histonet] Formalin asfety question In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF939553201E@chimsx08.CHI.catholichealth.net> References: <4F87EFE0.7400.0077.1@harthosp.org> <4940DF6D1C5FDF48931B6966AAEF939553201E@chimsx08.CHI.catholichealth.net> Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395532039@chimsx08.CHI.catholichealth.net> I withdraw the inquiry. Thanks -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, April 13, 2012 8:27 AM To: Histonet Subject: [Histonet] Formalin asfety question Is formalin an RCRA regulated waste? I am having some difficulty getting this answered and figured someone who is much brighter than myself out there may well know. If anyone has a reference, that would be a huge help William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 8:21 AM To: Histonet Subject: [Histonet] "know error system" - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From settembr <@t> umdnj.edu Fri Apr 13 10:02:31 2012 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Fri Apr 13 10:03:50 2012 Subject: [Histonet] New Reagent Lot Verification In-Reply-To: References: <3AD061FE740D464FAC7BF6B5CFB7570711FBC83F@SMCMAIL01.somerset-healthcare.com> Message-ID: We are saving slides from inspection to inspection, about 2 years. Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, April 12, 2012 11:26 AM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Reagent Lot Verification We're saving both for at least 2 years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, April 12, 2012 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Reagent Lot Verification ANP.22760 refers to new lot verification for antibodies and detection. The Evidence of Compliance says that there should be "Records of verification of new reagent lots". What is everyone's interpretation of "records"? Are you saving slides in addition to paper documentation? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Fri Apr 13 11:34:42 2012 From: louise.renton <@t> gmail.com (Louise Renton) Date: Fri Apr 13 11:34:49 2012 Subject: [Histonet] Wrinkles in whole bone mouse tibias In-Reply-To: <1334323849.98400.YahooMailNeo@web164503.mail.gq1.yahoo.com> References: <1334323849.98400.YahooMailNeo@web164503.mail.gq1.yahoo.com> Message-ID: Dear nancy what is the melting temp of the wax? perhaps your waterbath is too cool. Ideally you have have the temp so that the section expands but does not disintegrate. You could also try to pick up the wrinkly section on a slide, and then sort of "dab" it on a hotplate to "steam" out the wrinkles before you set the slide to drain. regards On Fri, Apr 13, 2012 at 3:30 PM, nancy lowen wrote: > Hello to all, > I am having on ongoing problem with wrinkles in the midshaft marrow area > of sections of whole mouse tibias. Leaving them in the waterbath longer > does not help--water bath temp. is 48 to 49 degrees. I have tried several > waxes to help, and am currently using McCormick Paraplast Plus. > Processing times are : 70%-1hour, 80% etoh,1hour, 95%-1hour, 3 100% > alcohols at 1 hour each. 2 Citrisolvs at 1:30 hours each, and four waxes > for 1hour, 1hour,1hour, and 3hours for the last. > Specimens seem to cut fine, and the head of the bone is good, but the > wrinkles are in the middle to lower part of the diaphysis. > Is is possible that the polymers in the wax could be the problem? > I see it mostly in the thinner, densely maarrow packed bones. > If anyone has any ideas, could you please pass it along, as I am getting > gray hairs from trying to figure it out. > Also, I do not fix the bones myself, but most of the bones I get have been > fixed for 24 hours in 10% Formalin. > Thanks in advance. > Nancy.lowen@Va.gov > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From Leisha_Machin <@t> URMC.Rochester.edu Fri Apr 13 13:24:46 2012 From: Leisha_Machin <@t> URMC.Rochester.edu (Machin, Leisha E) Date: Fri Apr 13 13:24:51 2012 Subject: [Histonet] Re: Wrinkles in whole bone mouse tibias Message-ID: <7C06700A9C2DA240A61046B23F5E84D2303E72FB@URMCMS3.urmc-sh.rochester.edu> Dear Nancy, I had your mouse tibia problem last year; the marrow consistently folded up accordion-style. I believe the problem was solved by placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes. Hope this help - best of luck! Leisha Machin "The capacity to blunder slightly is the real marvel of DNA. Without this special attribute, we would still be anaerobic bacteria and there would be no music." ~Lewis Thomas ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Friday, April 13, 2012 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 101, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. ACMS perspective on Aetna's new requirments. (Nicole Tatum) 2. Prepared STD slides? (Jon Krupp) 3. HistoTALK at the Region III Meeting (David Kemler) 4. Competency assesment forms for Pathologists (Sheila Adey) 5. Re: Prepared STD slides? (Kim Donadio) 6. Re: Prepared STD slides? (Lee & Peggy Wenk) 7. "know error system" - prostate biopsies (Richard Cartun) 8. RE: "know error system" - prostate biopsies (Lester Raff MD) 9. Formalin asfety question (O'Donnell, Bill) 10. Wrinkles in whole bone mouse tibias (nancy lowen) 11. RE: "know error system" - prostate biopsies (Heath, Nancy L.) 12. RE: Formalin asfety question (O'Donnell, Bill) 13. RE: New Reagent Lot Verification (Settembre, Dana) 14. Re: Wrinkles in whole bone mouse tibias (Louise Renton) ---------------------------------------------------------------------- Message: 1 Date: Thu, 12 Apr 2012 13:32:30 -0400 (EDT) From: "Nicole Tatum" Subject: [Histonet] ACMS perspective on Aetna's new requirments. To: histonet@lists.utsouthwestern.edu Message-ID: <2628.208.62.167.196.1334251950.squirrel@webmail.realpages.com> Content-Type: text/plain;charset=iso-8859-1 This is a letter From the American College of Mohs Surgery in reguards to Aetna letter for CAP requiremnet. April 5, 2012 I read your letter of March 23, 2012, outlining Aetna?s new requirement for CLIA and CAP certification for certain pathology services with dismay. I am very concerned these requirements could lead to lower cure rates, increased tissue loss and scarring, and even unnecessary deaths in your patients with problem skin cancer with Aetna insurance. CAP certification will be almost impossible to achieve for small Mohs Micrographic surgery frozen section laboratories. These labs can only be CAP certified if directed by an anatomic pathologist or dermatopathologist. Mohs surgeons, who have extensive additional training in pathology, and training to run their laboratories, do not fit into either niche. Mohs surgeons deal with the most difficult and recurrent skin cancers. Please find attached a copy of the CPT coding assistant from 2008 describing Mohs surgery, and detailing what services are included in it. With your new restrictions, immunohistochemistry (CPT code 88342) cannot be billed with Mohs surgery for malignant melanoma and spindle cell tumors. This will restrict the use of Mart 1 immuno stains and other special cytokeratin stains. This will result in lower cure rates and an increased number of deaths. These stains on additional frozen sections are not part of Mohs surgery, and are appropriately billed for separately. In addition, Mohs surgeons will not be able to bill for the occasional special stains on frozen sections (CPT code 8814) such as oil red O on a sebaceous carcinoma, or toluidine blue (in addition to the hematoxylin and Eosin stained frozen sections) to clarify an area of inflammation. Performing these special stains on ambiguous frozen sections often saves the patient additional surgery and tissue loss, and saves Aetna money, because another stage of Mohs surgery is avoided. These additional stains are not part of the Mohs surgery and are appropriately billed for separately. Denial of these CPT codes may result in more tissue being removed unnecessarily, lower cure rates, higher recurrence rates, and potentially deaths. Quality control of the frozen section laboratory is crucial, and mandatory for CAP lab approval. This quality control involves processing of ?confirming? formalin sections off the frozen blocks of tissue is commonly performed in Mohs surgery laboratories for quality control and confirmation. This will no longer be a billable service (CPT code 88305) per your letter. This directly contradicts CAP own recommendations for the follow up processing of frozen sections (see attached). These confirming formalin sections are not part of Mohs surgery and are separately billable. Your decision not to cover code CPT 88305 makes it impossible, or at a minimum, fiscally prohibitive, to maintain quality control and to even consider complying with the new CAP accreditation you are demanding in the same letter. >From the perspective of the American College of Mohs surgery, our patients could continue to receive the best quality of care, and the overall cost to Aetna may actually be lower (by avoiding additional stages of Mohs surgery), if CPT Codes 88314, 88305, and 88342 were exempted for providers who also bill the Mohs surgery CPT codes 17311 or 17313. Sincerely Brett Coldiron, M.D., F.A.C.P. President American College of Mohs Surgery ------------------------------ Message: 2 Date: Thu, 12 Apr 2012 12:48:34 -0700 From: Jon Krupp Subject: [Histonet] Prepared STD slides? To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hi I not a histo tech, nor do I play one on TV, so I might not be up to speed on this. A microbiology instructor asked me to see if there is a source of prepared slides of STD's that he could use in class. Maybe you know some place we could check? I have done some WWW searching, but have had a hard time finding prepared slides students could use with a microscope in class. Most everything seems to have gone digital and PowerPoint-ish. Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA 95207 209-954-5284 jkrupp@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College ------------------------------ Message: 3 Date: Thu, 12 Apr 2012 13:32:45 -0700 (PDT) From: David Kemler Subject: [Histonet] HistoTALK at the Region III Meeting To: Fellow HistoNetters Message-ID: <1334262765.35947.YahooMailNeo@web120602.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi HistoNetters - Tomorrow the HistoTALK "mobile" studio will be arriving late in the day?for the Region III Meeting at?Callaway Gardens, in Pine Mountain, GA,?We'll be there?to interview (it is not LIVE)?everyone and anyone daring to go before the HistoTALK microphone to be a guest on a later broadcast show. Even if you don't care to be a guest on the show, plan on stopping by on Saturday to say hi. ? There are plenty of open interview?times available on Saturday, so don't be shy! ? See you at the Region III Meeting which is hosted by my Georgia Society for Histotechnology friends. ? Yours, David? ------------------------------ Message: 4 Date: Thu, 12 Apr 2012 16:50:46 -0400 From: Sheila Adey Subject: [Histonet] Competency assesment forms for Pathologists To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello netters:Does anyone have an example of a competency assessment form designed for pathologists?Thanks in advance:)Sheila ------------------------------ Message: 5 Date: Thu, 12 Apr 2012 21:03:30 -0400 From: Kim Donadio Subject: Re: [Histonet] Prepared STD slides? To: Jon Krupp Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: <6E088F6E-6D78-43DD-A3A3-D9F2772C80E3@yahoo.com> Content-Type: text/plain; charset=us-ascii Contact your local college Might help Sent from my iPhone On Apr 12, 2012, at 3:48 PM, Jon Krupp wrote: > Hi > > I not a histo tech, nor do I play one on TV, so I might not be up to speed on this. > > A microbiology instructor asked me to see if there is a source of prepared slides of STD's that he could use in class. > > Maybe you know some place we could check? I have done some WWW searching, but have had a hard time finding prepared slides students could use with a microscope in class. Most everything seems to have gone digital and PowerPoint-ish. > > Thanks > > Jon > > Jonathan Krupp > Delta College > 5151 Pacific Ave. > Box 212 > Stockton, CA 95207 > 209-954-5284 > jkrupp@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 13 Apr 2012 05:03:33 -0400 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] Prepared STD slides? To: "Kim Donadio" , "Jon Krupp" Cc: Histonet@lists.utsouthwestern.edu Message-ID: <2DBC101318AF4479B45C1457B9AF4432@HP2010> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original A Google search of "prepared histology slides" found a company called Carolina http://www.carolina.com/ Click Life Sciences Click Microscopic slides Don't know if they have exactly what you want, but it's a start. Don't know anything about the company. Maybe someone on Histonet does. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 (Opinions expressed are mine, and do not reflect my place of employment.) -----Original Message----- From: Kim Donadio Sent: Thursday, April 12, 2012 9:03 PM To: Jon Krupp Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Prepared STD slides? Contact your local college Might help Sent from my iPhone On Apr 12, 2012, at 3:48 PM, Jon Krupp wrote: > Hi > > I not a histo tech, nor do I play one on TV, so I might not be up to speed > on this. > > A microbiology instructor asked me to see if there is a source of prepared > slides of STD's that he could use in class. > > Maybe you know some place we could check? I have done some WWW searching, > but have had a hard time finding prepared slides students could use with a > microscope in class. Most everything seems to have gone digital and > PowerPoint-ish. > > Thanks > > Jon > > Jonathan Krupp > Delta College > 5151 Pacific Ave. > Box 212 > Stockton, CA 95207 > 209-954-5284 > jkrupp@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 13 Apr 2012 09:20:32 -0400 From: "Richard Cartun" Subject: [Histonet] "know error system" - prostate biopsies To: "Histonet" Message-ID: <4F87EFE0.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ------------------------------ Message: 8 Date: Fri, 13 Apr 2012 08:24:43 -0500 From: "Lester Raff MD" Subject: RE: [Histonet] "know error system" - prostate biopsies To: "Richard Cartun" , "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" We do not! Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 8:21 AM To: Histonet Subject: [Histonet] "know error system" - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 13 Apr 2012 07:26:46 -0600 From: "O'Donnell, Bill" Subject: [Histonet] Formalin asfety question To: "Histonet" Message-ID: <4940DF6D1C5FDF48931B6966AAEF939553201E@chimsx08.CHI.catholichealth.net> Content-Type: text/plain; charset="ISO-8859-1" Is formalin an RCRA regulated waste? I am having some difficulty getting this answered and figured someone who is much brighter than myself out there may well know. If anyone has a reference, that would be a huge help William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 8:21 AM To: Histonet Subject: [Histonet] "know error system" - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 10 Date: Fri, 13 Apr 2012 06:30:49 -0700 (PDT) From: nancy lowen Subject: [Histonet] Wrinkles in whole bone mouse tibias To: "histonet@lists.utsouthwestern.edu" Message-ID: <1334323849.98400.YahooMailNeo@web164503.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello to all, I am having on ongoing problem with wrinkles in the midshaft marrow area of sections of whole mouse tibias.? Leaving them in the waterbath longer does not help--water bath temp. is 48 to 49 degrees.? I have tried several waxes to help, and am currently using McCormick Paraplast Plus. Processing times are : 70%-1hour, 80% etoh,1hour, 95%-1hour, 3 100% alcohols at 1 hour each. 2 Citrisolvs at 1:30 hours each, and four waxes for 1hour, 1hour,1hour, and 3hours for the last. Specimens seem to cut fine, and the head of the bone is good, but the wrinkles are in the middle to lower part of the diaphysis. Is is possible that the polymers in the wax could be the problem? I see it mostly in the thinner, densely maarrow packed bones. If anyone has any ideas, could you please pass it along, as I am getting gray hairs from trying to figure it out. Also, I do not fix the bones myself, but most of the bones I get have been fixed for 24 hours in 10% Formalin. Thanks in advance. Nancy.lowen@Va.gov ------------------------------ Message: 11 Date: Fri, 13 Apr 2012 09:41:01 -0400 From: "Heath, Nancy L." Subject: RE: [Histonet] "know error system" - prostate biopsies To: "Richard Cartun" , "Histonet" Message-ID: <130E8991F210424096EFC6F42EA33B2408D75B99@LSCOEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="us-ascii" When I worked per-diem for Plus Diagnostics here in Warwick RI we used it. It was a pain in the butt!! Nancy Heath, HT (ASCP) Neuropathology Technician Pathology Tech Specialist Dept. of Pathology., Div. of Neuropathology Rhode Island Hospital APC Blding, Flr 12, Rm 211 593 Eddy Street Providence, RI 02903 lab: 401-444-3246 fax: 401-444-8514 nheath@lifespan.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 9:21 AM To: Histonet Subject: [Histonet] "know error system" - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Fri, 13 Apr 2012 08:12:11 -0600 From: "O'Donnell, Bill" Subject: RE: [Histonet] Formalin asfety question To: "Histonet" Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395532039@chimsx08.CHI.catholichealth.net> Content-Type: text/plain; charset="ISO-8859-1" I withdraw the inquiry. Thanks -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, April 13, 2012 8:27 AM To: Histonet Subject: [Histonet] Formalin asfety question Is formalin an RCRA regulated waste? I am having some difficulty getting this answered and figured someone who is much brighter than myself out there may well know. If anyone has a reference, that would be a huge help William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 8:21 AM To: Histonet Subject: [Histonet] "know error system" - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 13 Date: Fri, 13 Apr 2012 11:02:31 -0400 From: "Settembre, Dana" Subject: RE: [Histonet] New Reagent Lot Verification To: "'Sebree Linda A'" , "Rathborne, Toni" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We are saving slides from inspection to inspection, about 2 years. Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, April 12, 2012 11:26 AM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Reagent Lot Verification We're saving both for at least 2 years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, April 12, 2012 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Reagent Lot Verification ANP.22760 refers to new lot verification for antibodies and detection. The Evidence of Compliance says that there should be "Records of verification of new reagent lots". What is everyone's interpretation of "records"? Are you saving slides in addition to paper documentation? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Fri, 13 Apr 2012 18:34:42 +0200 From: Louise Renton Subject: Re: [Histonet] Wrinkles in whole bone mouse tibias To: Histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Dear nancy what is the melting temp of the wax? perhaps your waterbath is too cool. Ideally you have have the temp so that the section expands but does not disintegrate. You could also try to pick up the wrinkly section on a slide, and then sort of "dab" it on a hotplate to "steam" out the wrinkles before you set the slide to drain. regards On Fri, Apr 13, 2012 at 3:30 PM, nancy lowen wrote: > Hello to all, > I am having on ongoing problem with wrinkles in the midshaft marrow area > of sections of whole mouse tibias. Leaving them in the waterbath longer > does not help--water bath temp. is 48 to 49 degrees. I have tried several > waxes to help, and am currently using McCormick Paraplast Plus. > Processing times are : 70%-1hour, 80% etoh,1hour, 95%-1hour, 3 100% > alcohols at 1 hour each. 2 Citrisolvs at 1:30 hours each, and four waxes > for 1hour, 1hour,1hour, and 3hours for the last. > Specimens seem to cut fine, and the head of the bone is good, but the > wrinkles are in the middle to lower part of the diaphysis. > Is is possible that the polymers in the wax could be the problem? > I see it mostly in the thinner, densely maarrow packed bones. > If anyone has any ideas, could you please pass it along, as I am getting > gray hairs from trying to figure it out. > Also, I do not fix the bones myself, but most of the bones I get have been > fixed for 24 hours in 10% Formalin. > Thanks in advance. > Nancy.lowen@Va.gov > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 101, Issue 18 ***************************************** From trathborne <@t> somerset-healthcare.com Fri Apr 13 13:26:39 2012 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Apr 13 13:26:11 2012 Subject: [Histonet] New Reagent Lot Verification In-Reply-To: References: <3AD061FE740D464FAC7BF6B5CFB7570711FBC83F@SMCMAIL01.somerset-healthcare.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711FBCB54@SMCMAIL01.somerset-healthcare.com> Thanks everyone! -----Original Message----- From: Settembre, Dana [mailto:settembr@umdnj.edu] Sent: Friday, April 13, 2012 11:03 AM To: 'Sebree Linda A'; Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Reagent Lot Verification We are saving slides from inspection to inspection, about 2 years. Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, April 12, 2012 11:26 AM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Reagent Lot Verification We're saving both for at least 2 years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, April 12, 2012 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Reagent Lot Verification ANP.22760 refers to new lot verification for antibodies and detection. The Evidence of Compliance says that there should be "Records of verification of new reagent lots". What is everyone's interpretation of "records"? Are you saving slides in addition to paper documentation? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From MBapardekar <@t> cbiolabs.com Fri Apr 13 14:36:51 2012 From: MBapardekar <@t> cbiolabs.com (Meghana Bapardekar) Date: Fri Apr 13 14:36:57 2012 Subject: [Histonet] RE: Wrinkles in whole bone mouse tibias In-Reply-To: <7C06700A9C2DA240A61046B23F5E84D2303E72FB@URMCMS3.urmc-sh.rochester.edu> References: <7C06700A9C2DA240A61046B23F5E84D2303E72FB@URMCMS3.urmc-sh.rochester.edu> Message-ID: <7D45E0BEEA3EF94FBCE61608C0CB63179A47EDFB@cbiolabs05.CBiolabs.local> Yes it should work, placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes as we experienced with similar samples. Hope this helps! Meghana V. Bapardekar, Ph. D., Dept. of Histopathology, Cleveland Biolabs, 73 High Street, Buffalo, NY - 14203. Phone: 716-849-6810, ext.: 357 Fax: 716-849-6817 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Machin, Leisha E Sent: Friday, April 13, 2012 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Wrinkles in whole bone mouse tibias Dear Nancy, I had your mouse tibia problem last year; the marrow consistently folded up accordion-style. I believe the problem was solved by placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes. Hope this help - best of luck! Leisha Machin This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From rjann3 <@t> verizon.net Fri Apr 13 15:29:17 2012 From: rjann3 <@t> verizon.net (Robert Jann) Date: Fri Apr 13 15:29:32 2012 Subject: [Histonet] "know error system" prostate biopsies Message-ID: <3BCF93974CFC488D827EB5AD6AA31642@HOMEPC> I work for a large urology group practice path lab in PA. It was pitched to us as a way of eliminating specimen errors from the numerous sites that sent us bx's. What we weren't told was the enormous amount of work involved or that it would fall on us to bill the insurances. Luckily, our medical director was able to persuade the urologists that it was neither needed or cost effective and we politely told know error, no thank you. Bob Jann HT(ASCP) From cpyse <@t> x-celllab.com Fri Apr 13 15:36:50 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri Apr 13 15:36:35 2012 Subject: [Histonet] "know error system" - prostate biopsies In-Reply-To: References: <4F87EFE0.7400.0077.1@harthosp.org> Message-ID: <002101cd19b5$2809ac70$781d0550$@com> We had one of our clients looking into this testing. The company does a hard sell. What they neglect to tell you is the insurance companies may not cover this testing leaving the patient with a huge bill sometimes $700 to $800. Known Error will tell you it is a Medicare approved test what they don't tell you is the patient will almost always end up with some bill, also the other HMO do not have approval leaving the patient with the full bill. Our client declined to use this system after further investigation form our lab. The amount of work evolved in processing and hands on specimen time is huge with no reimbursement to the lab itself. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Friday, April 13, 2012 9:25 AM To: Richard Cartun; Histonet Subject: RE: [Histonet] "know error system" - prostate biopsies We do not! Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 8:21 AM To: Histonet Subject: [Histonet] "know error system" - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kazquad1 <@t> yahoo.com Fri Apr 13 15:50:02 2012 From: kazquad1 <@t> yahoo.com (Kaz) Date: Fri Apr 13 15:50:15 2012 Subject: [Histonet] Unsubscribtion. Message-ID: <9luisy065paodhg19uwa8lr4.1334350042929@email.android.com> Like to unsubscribe. Thanks. Sent from my Samsung smartphone on AT&T From Diane.Tokugawa <@t> kp.org Fri Apr 13 17:15:10 2012 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Fri Apr 13 17:15:32 2012 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 04/13/2012 and will not return until 04/18/2012. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Mario at 8-421-4961, Kiran at 8-421-5404, or general histology client service at 8-421-5408. From tahseen <@t> brain.net.pk Sat Apr 14 00:17:51 2012 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Sat Apr 14 00:17:59 2012 Subject: [Histonet] New Reagent Lot Verification In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711FBCB54@SMCMAIL01.somerset-healthcar e.com> References: <3AD061FE740D464FAC7BF6B5CFB7570711FBC83F@SMCMAIL01.somerset-healthcare.com> <3AD061FE740D464FAC7BF6B5CFB7570711FBCB54@SMCMAIL01.somerset-healthcare.com> Message-ID: <21362.203.135.35.66.1334380671.squirrel@brain.net.pk> Dear All, Would you like to share format of verification form (new reagent lot/new antibody and new equipment). Thanks, Tahseen Histology supervisor SKMCH&RC Lahore > Thanks everyone! > > -----Original Message----- > From: Settembre, Dana [mailto:settembr@umdnj.edu] > Sent: Friday, April 13, 2012 11:03 AM > To: 'Sebree Linda A'; Rathborne, Toni; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New Reagent Lot Verification > > We are saving slides from inspection to inspection, about 2 years. > Dana Settembre > University Hospital - UMDNJ > Newark, NJ > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree > Linda A > Sent: Thursday, April 12, 2012 11:26 AM > To: Rathborne, Toni; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New Reagent Lot Verification > > We're saving both for at least 2 years. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, > Toni > Sent: Thursday, April 12, 2012 9:50 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Reagent Lot Verification > > ANP.22760 refers to new lot verification for antibodies and detection. > The Evidence of Compliance says that there should be "Records of > verification of new reagent lots". What is everyone's interpretation of > "records"? Are you saving slides in addition to paper documentation? > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in > this message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. > Unauthorized forwarding, printing, copying, distribution, or use of such > information is strictly prohibited and may be unlawful. If you are not > the addressee, please promptly delete this message and notify the sender > of the delivery error by e-mail or you may call Somerset Medical Center's > computer Help Desk at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, event > listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------------------------------------------------- > This message was secured by ZixCorp(R). > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in > this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Leisha_Machin <@t> URMC.Rochester.edu Sat Apr 14 14:03:22 2012 From: Leisha_Machin <@t> URMC.Rochester.edu (Machin, Leisha E) Date: Sat Apr 14 14:06:51 2012 Subject: [Histonet] RE: Wrinkles in whole bone mouse tibias Message-ID: <7C06700A9C2DA240A61046B23F5E84D2303E72FC@URMCMS3.urmc-sh.rochester.edu> Dear Nancy, Just wanted to add that you should face into the block before the soak in the water bath and transfer to ice-water. This method gave me about 15 good sections before needing to repeat the process. Again, best of luck! Leisha Machin Aab Cardiovascular Research Institute University of Rochester "The capacity to blunder slightly is the real marvel of DNA. Without this special attribute, we would still be anaerobic bacteria and there would be no music." ~Lewis Thomas ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Saturday, April 14, 2012 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 101, Issue 19 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Wrinkles in whole bone mouse tibias (Machin, Leisha E) 2. RE: New Reagent Lot Verification (Rathborne, Toni) 3. RE: Wrinkles in whole bone mouse tibias (Meghana Bapardekar) 4. "know error system" prostate biopsies (Robert Jann) 5. RE: "know error system" - prostate biopsies (Cynthia Pyse) 6. Unsubscribtion. (Kaz) 7. Diane Tokugawa/CA/KAIPERM is out of the office. (Diane.Tokugawa@kp.org) 8. RE: New Reagent Lot Verification (tahseen@brain.net.pk) ---------------------------------------------------------------------- Message: 1 Date: Fri, 13 Apr 2012 14:24:46 -0400 From: "Machin, Leisha E" Subject: [Histonet] Re: Wrinkles in whole bone mouse tibias To: "histonet@lists.utsouthwestern.edu" Message-ID: <7C06700A9C2DA240A61046B23F5E84D2303E72FB@URMCMS3.urmc-sh.rochester.edu> Content-Type: text/plain; charset="us-ascii" Dear Nancy, I had your mouse tibia problem last year; the marrow consistently folded up accordion-style. I believe the problem was solved by placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes. Hope this help - best of luck! Leisha Machin "The capacity to blunder slightly is the real marvel of DNA. Without this special attribute, we would still be anaerobic bacteria and there would be no music." ~Lewis Thomas ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Friday, April 13, 2012 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 101, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. ACMS perspective on Aetna's new requirments. (Nicole Tatum) 2. Prepared STD slides? (Jon Krupp) 3. HistoTALK at the Region III Meeting (David Kemler) 4. Competency assesment forms for Pathologists (Sheila Adey) 5. Re: Prepared STD slides? (Kim Donadio) 6. Re: Prepared STD slides? (Lee & Peggy Wenk) 7. "know error system" - prostate biopsies (Richard Cartun) 8. RE: "know error system" - prostate biopsies (Lester Raff MD) 9. Formalin asfety question (O'Donnell, Bill) 10. Wrinkles in whole bone mouse tibias (nancy lowen) 11. RE: "know error system" - prostate biopsies (Heath, Nancy L.) 12. RE: Formalin asfety question (O'Donnell, Bill) 13. RE: New Reagent Lot Verification (Settembre, Dana) 14. Re: Wrinkles in whole bone mouse tibias (Louise Renton) ---------------------------------------------------------------------- Message: 1 Date: Thu, 12 Apr 2012 13:32:30 -0400 (EDT) From: "Nicole Tatum" Subject: [Histonet] ACMS perspective on Aetna's new requirments. To: histonet@lists.utsouthwestern.edu Message-ID: <2628.208.62.167.196.1334251950.squirrel@webmail.realpages.com> Content-Type: text/plain;charset=iso-8859-1 This is a letter From the American College of Mohs Surgery in reguards to Aetna letter for CAP requiremnet. April 5, 2012 I read your letter of March 23, 2012, outlining Aetna?s new requirement for CLIA and CAP certification for certain pathology services with dismay. I am very concerned these requirements could lead to lower cure rates, increased tissue loss and scarring, and even unnecessary deaths in your patients with problem skin cancer with Aetna insurance. CAP certification will be almost impossible to achieve for small Mohs Micrographic surgery frozen section laboratories. These labs can only be CAP certified if directed by an anatomic pathologist or dermatopathologist. Mohs surgeons, who have extensive additional training in pathology, and training to run their laboratories, do not fit into either niche. Mohs surgeons deal with the most difficult and recurrent skin cancers. Please find attached a copy of the CPT coding assistant from 2008 describing Mohs surgery, and detailing what services are included in it. With your new restrictions, immunohistochemistry (CPT code 88342) cannot be billed with Mohs surgery for malignant melanoma and spindle cell tumors. This will restrict the use of Mart 1 immuno stains and other special cytokeratin stains. This will result in lower cure rates and an increased number of deaths. These stains on additional frozen sections are not part of Mohs surgery, and are appropriately billed for separately. In addition, Mohs surgeons will not be able to bill for the occasional special stains on frozen sections (CPT code 8814) such as oil red O on a sebaceous carcinoma, or toluidine blue (in addition to the hematoxylin and Eosin stained frozen sections) to clarify an area of inflammation. Performing these special stains on ambiguous frozen sections often saves the patient additional surgery and tissue loss, and saves Aetna money, because another stage of Mohs surgery is avoided. These additional stains are not part of the Mohs surgery and are appropriately billed for separately. Denial of these CPT codes may result in more tissue being removed unnecessarily, lower cure rates, higher recurrence rates, and potentially deaths. Quality control of the frozen section laboratory is crucial, and mandatory for CAP lab approval. This quality control involves processing of ?confirming? formalin sections off the frozen blocks of tissue is commonly performed in Mohs surgery laboratories for quality control and confirmation. This will no longer be a billable service (CPT code 88305) per your letter. This directly contradicts CAP own recommendations for the follow up processing of frozen sections (see attached). These confirming formalin sections are not part of Mohs surgery and are separately billable. Your decision not to cover code CPT 88305 makes it impossible, or at a minimum, fiscally prohibitive, to maintain quality control and to even consider complying with the new CAP accreditation you are demanding in the same letter. >From the perspective of the American College of Mohs surgery, our patients could continue to receive the best quality of care, and the overall cost to Aetna may actually be lower (by avoiding additional stages of Mohs surgery), if CPT Codes 88314, 88305, and 88342 were exempted for providers who also bill the Mohs surgery CPT codes 17311 or 17313. Sincerely Brett Coldiron, M.D., F.A.C.P. President American College of Mohs Surgery ------------------------------ Message: 2 Date: Thu, 12 Apr 2012 12:48:34 -0700 From: Jon Krupp Subject: [Histonet] Prepared STD slides? To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hi I not a histo tech, nor do I play one on TV, so I might not be up to speed on this. A microbiology instructor asked me to see if there is a source of prepared slides of STD's that he could use in class. Maybe you know some place we could check? I have done some WWW searching, but have had a hard time finding prepared slides students could use with a microscope in class. Most everything seems to have gone digital and PowerPoint-ish. Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA 95207 209-954-5284 jkrupp@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College ------------------------------ Message: 3 Date: Thu, 12 Apr 2012 13:32:45 -0700 (PDT) From: David Kemler Subject: [Histonet] HistoTALK at the Region III Meeting To: Fellow HistoNetters Message-ID: <1334262765.35947.YahooMailNeo@web120602.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi HistoNetters - Tomorrow the HistoTALK "mobile" studio will be arriving late in the day?for the Region III Meeting at?Callaway Gardens, in Pine Mountain, GA,?We'll be there?to interview (it is not LIVE)?everyone and anyone daring to go before the HistoTALK microphone to be a guest on a later broadcast show. Even if you don't care to be a guest on the show, plan on stopping by on Saturday to say hi. ? There are plenty of open interview?times available on Saturday, so don't be shy! ? See you at the Region III Meeting which is hosted by my Georgia Society for Histotechnology friends. ? Yours, David? ------------------------------ Message: 4 Date: Thu, 12 Apr 2012 16:50:46 -0400 From: Sheila Adey Subject: [Histonet] Competency assesment forms for Pathologists To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello netters:Does anyone have an example of a competency assessment form designed for pathologists?Thanks in advance:)Sheila ------------------------------ Message: 5 Date: Thu, 12 Apr 2012 21:03:30 -0400 From: Kim Donadio Subject: Re: [Histonet] Prepared STD slides? To: Jon Krupp Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: <6E088F6E-6D78-43DD-A3A3-D9F2772C80E3@yahoo.com> Content-Type: text/plain; charset=us-ascii Contact your local college Might help Sent from my iPhone On Apr 12, 2012, at 3:48 PM, Jon Krupp wrote: > Hi > > I not a histo tech, nor do I play one on TV, so I might not be up to speed on this. > > A microbiology instructor asked me to see if there is a source of prepared slides of STD's that he could use in class. > > Maybe you know some place we could check? I have done some WWW searching, but have had a hard time finding prepared slides students could use with a microscope in class. Most everything seems to have gone digital and PowerPoint-ish. > > Thanks > > Jon > > Jonathan Krupp > Delta College > 5151 Pacific Ave. > Box 212 > Stockton, CA 95207 > 209-954-5284 > jkrupp@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 13 Apr 2012 05:03:33 -0400 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] Prepared STD slides? To: "Kim Donadio" , "Jon Krupp" Cc: Histonet@lists.utsouthwestern.edu Message-ID: <2DBC101318AF4479B45C1457B9AF4432@HP2010> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original A Google search of "prepared histology slides" found a company called Carolina http://www.carolina.com/ Click Life Sciences Click Microscopic slides Don't know if they have exactly what you want, but it's a start. Don't know anything about the company. Maybe someone on Histonet does. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 (Opinions expressed are mine, and do not reflect my place of employment.) -----Original Message----- From: Kim Donadio Sent: Thursday, April 12, 2012 9:03 PM To: Jon Krupp Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Prepared STD slides? Contact your local college Might help Sent from my iPhone On Apr 12, 2012, at 3:48 PM, Jon Krupp wrote: > Hi > > I not a histo tech, nor do I play one on TV, so I might not be up to speed > on this. > > A microbiology instructor asked me to see if there is a source of prepared > slides of STD's that he could use in class. > > Maybe you know some place we could check? I have done some WWW searching, > but have had a hard time finding prepared slides students could use with a > microscope in class. Most everything seems to have gone digital and > PowerPoint-ish. > > Thanks > > Jon > > Jonathan Krupp > Delta College > 5151 Pacific Ave. > Box 212 > Stockton, CA 95207 > 209-954-5284 > jkrupp@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 13 Apr 2012 09:20:32 -0400 From: "Richard Cartun" Subject: [Histonet] "know error system" - prostate biopsies To: "Histonet" Message-ID: <4F87EFE0.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ------------------------------ Message: 8 Date: Fri, 13 Apr 2012 08:24:43 -0500 From: "Lester Raff MD" Subject: RE: [Histonet] "know error system" - prostate biopsies To: "Richard Cartun" , "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" We do not! Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 8:21 AM To: Histonet Subject: [Histonet] "know error system" - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 13 Apr 2012 07:26:46 -0600 From: "O'Donnell, Bill" Subject: [Histonet] Formalin asfety question To: "Histonet" Message-ID: <4940DF6D1C5FDF48931B6966AAEF939553201E@chimsx08.CHI.catholichealth.net> Content-Type: text/plain; charset="ISO-8859-1" Is formalin an RCRA regulated waste? I am having some difficulty getting this answered and figured someone who is much brighter than myself out there may well know. If anyone has a reference, that would be a huge help William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 8:21 AM To: Histonet Subject: [Histonet] "know error system" - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 10 Date: Fri, 13 Apr 2012 06:30:49 -0700 (PDT) From: nancy lowen Subject: [Histonet] Wrinkles in whole bone mouse tibias To: "histonet@lists.utsouthwestern.edu" Message-ID: <1334323849.98400.YahooMailNeo@web164503.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello to all, I am having on ongoing problem with wrinkles in the midshaft marrow area of sections of whole mouse tibias.? Leaving them in the waterbath longer does not help--water bath temp. is 48 to 49 degrees.? I have tried several waxes to help, and am currently using McCormick Paraplast Plus. Processing times are : 70%-1hour, 80% etoh,1hour, 95%-1hour, 3 100% alcohols at 1 hour each. 2 Citrisolvs at 1:30 hours each, and four waxes for 1hour, 1hour,1hour, and 3hours for the last. Specimens seem to cut fine, and the head of the bone is good, but the wrinkles are in the middle to lower part of the diaphysis. Is is possible that the polymers in the wax could be the problem? I see it mostly in the thinner, densely maarrow packed bones. If anyone has any ideas, could you please pass it along, as I am getting gray hairs from trying to figure it out. Also, I do not fix the bones myself, but most of the bones I get have been fixed for 24 hours in 10% Formalin. Thanks in advance. Nancy.lowen@Va.gov ------------------------------ Message: 11 Date: Fri, 13 Apr 2012 09:41:01 -0400 From: "Heath, Nancy L." Subject: RE: [Histonet] "know error system" - prostate biopsies To: "Richard Cartun" , "Histonet" Message-ID: <130E8991F210424096EFC6F42EA33B2408D75B99@LSCOEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="us-ascii" When I worked per-diem for Plus Diagnostics here in Warwick RI we used it. It was a pain in the butt!! Nancy Heath, HT (ASCP) Neuropathology Technician Pathology Tech Specialist Dept. of Pathology., Div. of Neuropathology Rhode Island Hospital APC Blding, Flr 12, Rm 211 593 Eddy Street Providence, RI 02903 lab: 401-444-3246 fax: 401-444-8514 nheath@lifespan.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 9:21 AM To: Histonet Subject: [Histonet] "know error system" - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Fri, 13 Apr 2012 08:12:11 -0600 From: "O'Donnell, Bill" Subject: RE: [Histonet] Formalin asfety question To: "Histonet" Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395532039@chimsx08.CHI.catholichealth.net> Content-Type: text/plain; charset="ISO-8859-1" I withdraw the inquiry. Thanks -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, April 13, 2012 8:27 AM To: Histonet Subject: [Histonet] Formalin asfety question Is formalin an RCRA regulated waste? I am having some difficulty getting this answered and figured someone who is much brighter than myself out there may well know. If anyone has a reference, that would be a huge help William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 8:21 AM To: Histonet Subject: [Histonet] "know error system" - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 13 Date: Fri, 13 Apr 2012 11:02:31 -0400 From: "Settembre, Dana" Subject: RE: [Histonet] New Reagent Lot Verification To: "'Sebree Linda A'" , "Rathborne, Toni" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We are saving slides from inspection to inspection, about 2 years. Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, April 12, 2012 11:26 AM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Reagent Lot Verification We're saving both for at least 2 years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, April 12, 2012 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Reagent Lot Verification ANP.22760 refers to new lot verification for antibodies and detection. The Evidence of Compliance says that there should be "Records of verification of new reagent lots". What is everyone's interpretation of "records"? Are you saving slides in addition to paper documentation? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Fri, 13 Apr 2012 18:34:42 +0200 From: Louise Renton Subject: Re: [Histonet] Wrinkles in whole bone mouse tibias To: Histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Dear nancy what is the melting temp of the wax? perhaps your waterbath is too cool. Ideally you have have the temp so that the section expands but does not disintegrate. You could also try to pick up the wrinkly section on a slide, and then sort of "dab" it on a hotplate to "steam" out the wrinkles before you set the slide to drain. regards On Fri, Apr 13, 2012 at 3:30 PM, nancy lowen wrote: > Hello to all, > I am having on ongoing problem with wrinkles in the midshaft marrow area > of sections of whole mouse tibias. Leaving them in the waterbath longer > does not help--water bath temp. is 48 to 49 degrees. I have tried several > waxes to help, and am currently using McCormick Paraplast Plus. > Processing times are : 70%-1hour, 80% etoh,1hour, 95%-1hour, 3 100% > alcohols at 1 hour each. 2 Citrisolvs at 1:30 hours each, and four waxes > for 1hour, 1hour,1hour, and 3hours for the last. > Specimens seem to cut fine, and the head of the bone is good, but the > wrinkles are in the middle to lower part of the diaphysis. > Is is possible that the polymers in the wax could be the problem? > I see it mostly in the thinner, densely maarrow packed bones. > If anyone has any ideas, could you please pass it along, as I am getting > gray hairs from trying to figure it out. > Also, I do not fix the bones myself, but most of the bones I get have been > fixed for 24 hours in 10% Formalin. > Thanks in advance. > Nancy.lowen@Va.gov > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 101, Issue 18 ***************************************** ------------------------------ Message: 2 Date: Fri, 13 Apr 2012 18:26:39 +0000 From: "Rathborne, Toni" Subject: RE: [Histonet] New Reagent Lot Verification To: "'Settembre, Dana'" , "'Sebree Linda A'" , "histonet@lists.utsouthwestern.edu" Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711FBCB54@SMCMAIL01.somerset-healthcare.com> Content-Type: text/plain; charset="us-ascii" Thanks everyone! -----Original Message----- From: Settembre, Dana [mailto:settembr@umdnj.edu] Sent: Friday, April 13, 2012 11:03 AM To: 'Sebree Linda A'; Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Reagent Lot Verification We are saving slides from inspection to inspection, about 2 years. Dana Settembre University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, April 12, 2012 11:26 AM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Reagent Lot Verification We're saving both for at least 2 years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, April 12, 2012 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Reagent Lot Verification ANP.22760 refers to new lot verification for antibodies and detection. The Evidence of Compliance says that there should be "Records of verification of new reagent lots". What is everyone's interpretation of "records"? Are you saving slides in addition to paper documentation? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 3 Date: Fri, 13 Apr 2012 19:36:51 +0000 From: "Meghana Bapardekar" Subject: [Histonet] RE: Wrinkles in whole bone mouse tibias To: "Machin, Leisha E" , Message-ID: <7D45E0BEEA3EF94FBCE61608C0CB63179A47EDFB@cbiolabs05.CBiolabs.local> Content-Type: text/plain; charset="us-ascii" Yes it should work, placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes as we experienced with similar samples. Hope this helps! Meghana V. Bapardekar, Ph. D., Dept. of Histopathology, Cleveland Biolabs, 73 High Street, Buffalo, NY - 14203. Phone: 716-849-6810, ext.: 357 Fax: 716-849-6817 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Machin, Leisha E Sent: Friday, April 13, 2012 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Wrinkles in whole bone mouse tibias Dear Nancy, I had your mouse tibia problem last year; the marrow consistently folded up accordion-style. I believe the problem was solved by placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes. Hope this help - best of luck! Leisha Machin This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ------------------------------ Message: 4 Date: Fri, 13 Apr 2012 16:29:17 -0400 From: "Robert Jann" Subject: [Histonet] "know error system" prostate biopsies To: "Histonet" Message-ID: <3BCF93974CFC488D827EB5AD6AA31642@HOMEPC> Content-Type: text/plain; charset="iso-8859-1" I work for a large urology group practice path lab in PA. It was pitched to us as a way of eliminating specimen errors from the numerous sites that sent us bx's. What we weren't told was the enormous amount of work involved or that it would fall on us to bill the insurances. Luckily, our medical director was able to persuade the urologists that it was neither needed or cost effective and we politely told know error, no thank you. Bob Jann HT(ASCP) ------------------------------ Message: 5 Date: Fri, 13 Apr 2012 16:36:50 -0400 From: "Cynthia Pyse" Subject: RE: [Histonet] "know error system" - prostate biopsies To: "'Lester Raff MD'" , "'Richard Cartun'" , "'Histonet'" Message-ID: <002101cd19b5$2809ac70$781d0550$@com> Content-Type: text/plain; charset="us-ascii" We had one of our clients looking into this testing. The company does a hard sell. What they neglect to tell you is the insurance companies may not cover this testing leaving the patient with a huge bill sometimes $700 to $800. Known Error will tell you it is a Medicare approved test what they don't tell you is the patient will almost always end up with some bill, also the other HMO do not have approval leaving the patient with the full bill. Our client declined to use this system after further investigation form our lab. The amount of work evolved in processing and hands on specimen time is huge with no reimbursement to the lab itself. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Friday, April 13, 2012 9:25 AM To: Richard Cartun; Histonet Subject: RE: [Histonet] "know error system" - prostate biopsies We do not! Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 8:21 AM To: Histonet Subject: [Histonet] "know error system" - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the "know error system" for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 13 Apr 2012 13:50:02 -0700 From: Kaz Subject: [Histonet] Unsubscribtion. To: 'Histonet' Message-ID: <9luisy065paodhg19uwa8lr4.1334350042929@email.android.com> Content-Type: text/plain; charset=utf-8 Like to unsubscribe. Thanks. Sent from my Samsung smartphone on AT&T ------------------------------ Message: 7 Date: Fri, 13 Apr 2012 15:15:10 -0700 From: Diane.Tokugawa@kp.org Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 04/13/2012 and will not return until 04/18/2012. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology / IHC issues, please call Mario at 8-421-4961, Kiran at 8-421-5404, or general histology client service at 8-421-5408. ------------------------------ Message: 8 Date: Sat, 14 Apr 2012 10:17:51 +0500 (PKT) From: tahseen@brain.net.pk Subject: RE: [Histonet] New Reagent Lot Verification To: "Rathborne, Toni" Cc: "histonet@lists.utsouthwestern.edu" , 'Sebree Linda A' Message-ID: <21362.203.135.35.66.1334380671.squirrel@brain.net.pk> Content-Type: text/plain;charset=iso-8859-1 Dear All, Would you like to share format of verification form (new reagent lot/new antibody and new equipment). Thanks, Tahseen Histology supervisor SKMCH&RC Lahore > Thanks everyone! > > -----Original Message----- > From: Settembre, Dana [mailto:settembr@umdnj.edu] > Sent: Friday, April 13, 2012 11:03 AM > To: 'Sebree Linda A'; Rathborne, Toni; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New Reagent Lot Verification > > We are saving slides from inspection to inspection, about 2 years. > Dana Settembre > University Hospital - UMDNJ > Newark, NJ > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree > Linda A > Sent: Thursday, April 12, 2012 11:26 AM > To: Rathborne, Toni; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New Reagent Lot Verification > > We're saving both for at least 2 years. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, > Toni > Sent: Thursday, April 12, 2012 9:50 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Reagent Lot Verification > > ANP.22760 refers to new lot verification for antibodies and detection. > The Evidence of Compliance says that there should be "Records of > verification of new reagent lots". What is everyone's interpretation of > "records"? Are you saving slides in addition to paper documentation? > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in > this message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. > Unauthorized forwarding, printing, copying, distribution, or use of such > information is strictly prohibited and may be unlawful. If you are not > the addressee, please promptly delete this message and notify the sender > of the delivery error by e-mail or you may call Somerset Medical Center's > computer Help Desk at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, event > listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------------------------------------------------- > This message was secured by ZixCorp(R). > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in > this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 101, Issue 19 ***************************************** From alonso.martinezcanabal <@t> utoronto.ca Mon Apr 16 10:26:47 2012 From: alonso.martinezcanabal <@t> utoronto.ca (Alonso Martinez-Canabal) Date: Mon Apr 16 10:27:09 2012 Subject: [Histonet] Methyl green going away... Message-ID: <000901cd1be5$5c48d470$14da7d50$@utoronto.ca> Hi my boss requires me to counterstain some DAB immunohistochemistry with methyl green. I use for that purpose the methyl green in aquos solution 0.5% in 4.2 acetate buffer. The problem is that after the washes I dehydrate and the methyl green becomes extremely faint with the alcohols. My protocol requires long periods of alcoholic dehydration very graded. Is there any idea on how to save the methyl green. Can I use an alcoholic solution? Thank you very much. Alonso From schaundrawalton <@t> yahoo.com Mon Apr 16 10:33:56 2012 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Mon Apr 16 10:33:59 2012 Subject: [Histonet] CAP Question Message-ID: <1334590436.27960.YahooMailNeo@web120601.mail.ne1.yahoo.com> I have a question regarding a new CAP checklist question dealing with ER/PR validation.? The new question is ANP.22976 ER/PgR Validation and states: ? ?If the laboratory performs immunohistochemistry for estrogen receptor (ER) and/or progesterone receptor (PgR) as a prognostic/predictive marker on breast carcinoma, the laboratory has documented appropriate validation for the assay(s). ?Note: Initial test validation should include a minimum of 40 cases (20 positive and 20 negative cases) for FDA-approved/cleared tests; laboratories should consider using higher numbers of test cases if a Laboratory Developed or Laboratory Modified Test is to be validated.? Validation should be performed by comparing the laboratory?s results with another assay that has been appropriately validated.? Acceptable concordance levels are 90% for positive results and 95% for negative results.? If significant changes are made to the testing methods (e.g. antibody clones, antigen retrieval protocol or detection system) revalidation is required. ? ? Our original validation was done in 2005 and was performed with 20 cases.? Do we need to revalidate to be compliant with this question?? Should we start from scratch or just do an additional 20 cases?? ? Any feedback is appreciated. ? Thanks!? Schaundra Walton BS HTL(ASCP) Histology Supervisor From BHAVICE <@t> lewistownhospital.org Mon Apr 16 10:41:26 2012 From: BHAVICE <@t> lewistownhospital.org (Havice, Rebecca) Date: Mon Apr 16 10:41:36 2012 Subject: [Histonet] volunteer opportunity Message-ID: My name is Becky Havice and I am a registered Cytotechnologist as well as a registered Histotechnician. I just returned from a week of volunteering at the CerviCusco clinic in Cusco Peru. I signed on to work as a Cytotechnologist, but found they had a great need for a histotech, so I spent the week in the "histology department". CerviCusco clinic is run by the International Cervical Cancer (INCCA) foundation's humanitarian program in Peru. INCCA's goal is to reduce the high incidence of cervical cancer in the Andes Mountains region of Peru. They also provide other women's healthcare services. In just 5 years, INCCA has provided medical services for more than 15,000 indigent women. More than 200 volunteers representing 7 countries have participated. Currently, volunteer pathologists, cytotechnologists and family members volunteer at the clinic 4 times a year. After this past week, it is apparent they are also in need of volunteer histotechs. This past week was a very rewarding experience for me. I was able to use my abilities as a histotech in a histology environment that was very basic, but also very much needed. If you would be interested in volunteering your services or would just like more information on the CerviCusco clinic, please contact: Barbara Winkler, MD Medical Director of Pathology, Mount Kisco Medical Group Phone 914-302-8322 bwinkler@mkmg.com Your help would be greatly appreciated. From claycal44 <@t> yahoo.com Mon Apr 16 10:45:28 2012 From: claycal44 <@t> yahoo.com (nancy lowen) Date: Mon Apr 16 10:45:53 2012 Subject: [Histonet] A big thanks Message-ID: <1334591128.44693.YahooMailNeo@web164502.mail.gq1.yahoo.com> I would like to say a big thank you to everyone who replied and sent me ideas to get the wrinkles out of the diaphysis of the mouse tibias I was cutting. Will be trying out some of the tips I received. Thanks again Nancy Lowen From settembr <@t> umdnj.edu Mon Apr 16 11:07:21 2012 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Mon Apr 16 11:07:29 2012 Subject: [Histonet] CAP Question -ER/PR Validation In-Reply-To: <1334590436.27960.YahooMailNeo@web120601.mail.ne1.yahoo.com> References: <1334590436.27960.YahooMailNeo@web120601.mail.ne1.yahoo.com> Message-ID: You should pose that question to CAP via accred@cap.org they answer quickly and when they do you can print out their answer and keep for when your inspectors come. When I asked them that question, they said that it was usually at the discretion of the pathologist in charge. But instead of taking my word for It, email them and when you receive their response, print it. Then follow their instructions. Dana Settembre -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Monday, April 16, 2012 11:34 AM To: Histonet Subject: [Histonet] CAP Question I have a question regarding a new CAP checklist question dealing with ER/PR validation.? The new question is ANP.22976 ER/PgR Validation and states: ? "If the laboratory performs immunohistochemistry for estrogen receptor (ER) and/or progesterone receptor (PgR) as a prognostic/predictive marker on breast carcinoma, the laboratory has documented appropriate validation for the assay(s). ?Note: Initial test validation should include a minimum of 40 cases (20 positive and 20 negative cases) for FDA-approved/cleared tests; laboratories should consider using higher numbers of test cases if a Laboratory Developed or Laboratory Modified Test is to be validated.? Validation should be performed by comparing the laboratory's results with another assay that has been appropriately validated.? Acceptable concordance levels are 90% for positive results and 95% for negative results.? If significant changes are made to the testing methods (e.g. antibody clones, antigen retrieval protocol or detection system) revalidation is required. " ? Our original validation was done in 2005 and was performed with 20 cases.? Do we need to revalidate to be compliant with this question?? Should we start from scratch or just do an additional 20 cases?? ? Any feedback is appreciated. ? Thanks!? Schaundra Walton BS HTL(ASCP) Histology Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Mon Apr 16 11:32:40 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Apr 16 11:32:54 2012 Subject: [Histonet] CAP Question In-Reply-To: <1334590436.27960.YahooMailNeo@web120601.mail.ne1.yahoo.com> References: <1334590436.27960.YahooMailNeo@web120601.mail.ne1.yahoo.com> Message-ID: <8D7C2D242DBD45498006B21122072BF8A5064F6D@MCINFRWEM003.ucsfmedicalcenter.org> Shaundra, The CAP requirements are for those who are starting ER/PR for the first time, or changing antibodies to a new clone. If you did the validation years ago before those recommendations came out and have a history of running it successfully then you do not have to re-validate the procedure. If you change antibodies to new clones, then you would have to do the more extensive validation. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Monday, April 16, 2012 8:34 AM To: Histonet Subject: [Histonet] CAP Question I have a question regarding a new CAP checklist question dealing with ER/PR validation.? The new question is ANP.22976 ER/PgR Validation and states: ? ?If the laboratory performs immunohistochemistry for estrogen receptor (ER) and/or progesterone receptor (PgR) as a prognostic/predictive marker on breast carcinoma, the laboratory has documented appropriate validation for the assay(s). ?Note: Initial test validation should include a minimum of 40 cases (20 positive and 20 negative cases) for FDA-approved/cleared tests; laboratories should consider using higher numbers of test cases if a Laboratory Developed or Laboratory Modified Test is to be validated.? Validation should be performed by comparing the laboratory?s results with another assay that has been appropriately validated. Acceptable concordance levels are 90% for positive results and 95% for negative results.? If significant changes are made to the testing methods (e.g. antibody clones, antigen retrieval protocol or detection system) revalidation is required. ? ? Our original validation was done in 2005 and was performed with 20 cases.? Do we need to revalidate to be compliant with this question?? Should we start from scratch or just do an additional 20 cases?? ? Any feedback is appreciated. ? Thanks! Schaundra Walton BS HTL(ASCP) Histology Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Mon Apr 16 11:38:21 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Mon Apr 16 11:38:30 2012 Subject: [Histonet] Methyl green going away... In-Reply-To: <000901cd1be5$5c48d470$14da7d50$@utoronto.ca> References: <000901cd1be5$5c48d470$14da7d50$@utoronto.ca> Message-ID: I don't know if this will apply to your application, but after the chromogen and counterstaining steps, we rinse with distilled water and air dry our IHC slides (oven at 60C for 30 min) rather than dehydration through alcohol and xylene. Works great with permanent red and hematoxylin. Good luck, Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alonso Martinez-Canabal Sent: Monday, April 16, 2012 9:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Methyl green going away... Hi my boss requires me to counterstain some DAB immunohistochemistry with methyl green. I use for that purpose the methyl green in aquos solution 0.5% in 4.2 acetate buffer. The problem is that after the washes I dehydrate and the methyl green becomes extremely faint with the alcohols. My protocol requires long periods of alcoholic dehydration very graded. Is there any idea on how to save the methyl green. Can I use an alcoholic solution? Thank you very much. Alonso _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeleinehuey <@t> gmail.com Mon Apr 16 12:36:16 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Mon Apr 16 12:36:21 2012 Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 21 In-Reply-To: <4f8c5033.e3063c0a.48cf.2347SMTPIN_ADDED@mx.google.com> References: <4f8c5033.e3063c0a.48cf.2347SMTPIN_ADDED@mx.google.com> Message-ID: > Message: 1 > Date: Mon, 16 Apr 2012 11:26:47 -0400 > From: "Alonso Martinez-Canabal" > Subject: [Histonet] Methyl green going away... > To: > Message-ID: <000901cd1be5$5c48d470$14da7d50$@utoronto.ca> > Content-Type: text/plain; ? ? ? charset="US-ASCII" > > ?Hi > > my boss requires me to counterstain some DAB immunohistochemistry with > methyl green. I use for that purpose the methyl ?green in aquos solution > 0.5% in 4.2 acetate buffer. The problem is that after the washes I dehydrate > and the methyl green becomes extremely faint with the alcohols. My protocol > requires long periods of alcoholic dehydration very graded. Is there any > idea on how to save the methyl green. Can I use an alcoholic solution? > Thank you very much. > > Alonso Alonso, Another alternative is; Drying the slide in the oven @~ 60c for 10 min after the methly green counterstain. Madeleine Huey BS, HTL/QIHC (ASCP) Supervisor - Pathology (IPOX & Histology) madeleinehuey@elcaminohospital.org From GauchV <@t> mail.amc.edu Mon Apr 16 13:07:09 2012 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Mon Apr 16 13:07:19 2012 Subject: [Histonet] Tissue Processor Message-ID: Hi everyone, I was wondering if anyone had any input on the Logos tissue processor by Milestone. Is anyone currently using it and what are the pros and cons, etc. . Any information would be greatly appreciated. Thanks, Vicki Gauch AMCH Albany, NY From galinadeyneko <@t> yahoo.com Mon Apr 16 14:00:29 2012 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Mon Apr 16 14:00:33 2012 Subject: [Histonet] Re: IHC in porcine tissues Message-ID: <1334602829.4354.YahooMailClassic@web161004.mail.bf1.yahoo.com> Dear Colleagues ?What? secondary antibody or polymer would be better to use for mouse primary antibody?on FFPE ?porcine tissues?Also, please share your experience regarding macrophages detection in porcine arteries. I found couple in Abcam and Serotec (only for frozen), but your opinions is very valuable. Thank you Galina Deyneko Novartis, Cambridge, MA 617-782-1675 home 617-871-7613 w From liz <@t> premierlab.com Mon Apr 16 14:10:43 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Apr 16 14:10:52 2012 Subject: [Histonet] Re: IHC in porcine tissues In-Reply-To: <1334602829.4354.YahooMailClassic@web161004.mail.bf1.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC5BDF@SBS2K8.premierlab.local> Galina Try MAC387 that is a macrophage/monocyte marker that cross reacts in porcine. We use the one from abcam. We have found that some of the anti-mouse polymer reagents cross react to porcine so we use a rabbit anti-mouse from Dako E0464 as a secondary and then an envision rabbit or strept avidin and that works much better. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galina Deyneko Sent: Monday, April 16, 2012 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: IHC in porcine tissues Dear Colleagues What secondary antibody or polymer would be better to use for mouse primary antibody on FFPE porcine tissues?Also, please share your experience regarding macrophages detection in porcine arteries. I found couple in Abcam and Serotec (only for frozen), but your opinions is very valuable. Thank you Galina Deyneko Novartis, Cambridge, MA 617-782-1675 home 617-871-7613 w _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Mon Apr 16 19:49:44 2012 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Apr 16 19:49:53 2012 Subject: [Histonet] Methyl green going away... In-Reply-To: <000901cd1be5$5c48d470$14da7d50$@utoronto.ca> References: <000901cd1be5$5c48d470$14da7d50$@utoronto.ca> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A41B0E@xmdb02.nch.kids> You could try dehydrating in acetone (very quick). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alonso Martinez-Canabal Sent: Tuesday, 17 April 2012 1:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Methyl green going away... Hi my boss requires me to counterstain some DAB immunohistochemistry with methyl green. I use for that purpose the methyl green in aquos solution 0.5% in 4.2 acetate buffer. The problem is that after the washes I dehydrate and the methyl green becomes extremely faint with the alcohols. My protocol requires long periods of alcoholic dehydration very graded. Is there any idea on how to save the methyl green. Can I use an alcoholic solution? Thank you very much. Alonso _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Neil.Macintyre <@t> ed.ac.uk Tue Apr 17 11:25:36 2012 From: Neil.Macintyre <@t> ed.ac.uk (Neil Macintyre) Date: Tue Apr 17 11:25:50 2012 Subject: [Histonet] Citrate Buffer pH2.0 Message-ID: <201204171625.q3HGPdis011819@lmtp1.ucs.ed.ac.uk> Hi Can anyone help me with a formula for 0.01M citrate buffer pH2.0 for antigen retrieval as this pH has been recommended by the company supplying AM3K macrophage antibody and I can't find this in any of the usual IHC buffer protocols. Thanks Neil MacIntyre CSci FIBMS Laboratory Manager Veterinary Pathology Unit The Royal(Dick)School of Veterinary Studies The University Of Edinburgh Easter Bush Campus Midlothian EH25 9RG 0131 650 6403/8802 The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336. From LSebree <@t> uwhealth.org Tue Apr 17 11:34:41 2012 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Apr 17 11:34:48 2012 Subject: [Histonet] Reference lab needed for Alzheimer antibodies Message-ID: Good morning Histonetters, For purposes of validation, I'm looking for a reference lab that performs IHC on human FFPE samples for several Alzheimer antibodies. Specifically these antibodies are: PHF-1, CP13, anti-TDP-43 and LB 509 10. I'd also be interested in any private/hospital/research labs performing these. Thanks, Linda From Eric.Hoy <@t> UTSouthwestern.edu Tue Apr 17 11:48:30 2012 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Tue Apr 17 11:48:41 2012 Subject: [Histonet] Citrate Buffer pH2.0 In-Reply-To: <9734b6290aef40e4a058c9f7b234a409@SWMSHUB3.swmed.org> Message-ID: Why do you want the pH so low? Citrate buffers are most effective in the range of pH 3.0-6.0. I would check with the company and make sure that the pH is supposed to be 2.0, and ask them for a formula to achieve such a solution. It probably won't have much buffering capacity at pH 2.0. There is a Java application that will calculate formulae for citrate buffers, but it will point out that the buffer should be used close to one of the three pK's of citric acid: http://www.egr.msu.edu/scb-group/tools/citric/cit.htm Good luck! Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== On 4/17/12 11:25 AM, "Neil Macintyre" wrote: > Hi > Can anyone help me with a formula for 0.01M citrate buffer pH2.0 for antigen > retrieval as this pH has been recommended by the company supplying AM3K > macrophage antibody and I can't find this in any of the usual IHC buffer > protocols. > Thanks > From TGoins <@t> mt.gov Tue Apr 17 12:00:43 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Tue Apr 17 12:01:02 2012 Subject: [Histonet] Citrate Buffer pH2.0 In-Reply-To: <201204171625.q3HGPdis011819@lmtp1.ucs.ed.ac.uk> References: <201204171625.q3HGPdis011819@lmtp1.ucs.ed.ac.uk> Message-ID: Neil: I haven't made stock solution recently, but I believe the starting pH of solution prior to adding NaOH is less than 2, so this should work. Citrate pH 3.0 use at 10 mM For a 10 x stock solution [100 mM] 10.5 g citric acid monohydrate [MW 210.14] in 370 ml water adjust the pH to 3.0 with concentrated NaOH add 2.5 ml Tween 20 adjust final volume to 500 ml Tresa Tresa Goins Montana Veterinary Diagnostic Lab Bozeman, Montana 59718 406-994-6353 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Neil Macintyre Sent: Tuesday, April 17, 2012 10:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Citrate Buffer pH2.0 Hi Can anyone help me with a formula for 0.01M citrate buffer pH2.0 for antigen retrieval as this pH has been recommended by the company supplying AM3K macrophage antibody and I can't find this in any of the usual IHC buffer protocols. Thanks Neil MacIntyre CSci FIBMS Laboratory Manager Veterinary Pathology Unit The Royal(Dick)School of Veterinary Studies The University Of Edinburgh Easter Bush Campus Midlothian EH25 9RG 0131 650 6403/8802 The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From galinadeyneko <@t> yahoo.com Tue Apr 17 12:48:55 2012 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Tue Apr 17 12:49:02 2012 Subject: [Histonet] Re: IHC in porcine tissues In-Reply-To: <14E2C6176416974295479C64A11CB9AE011390CC5BDF@SBS2K8.premierlab.local> Message-ID: <1334684935.3041.YahooMailClassic@web161003.mail.bf1.yahoo.com> Dear Elizabeth and Colleagues I found in Abcam two ABs clone MAC387 : ab 22506 (with your reference) and ab80708. Also if somebody interested I found new polymer from Biocare: mouse-on-pharma HRP polymer that is intended to work in bovine, porcine,ovine tissues. Not expensive- 231$. I am going to try. Thank you all for your responses. Galina Deyneko Novartis, Cambridge, MA 617-782-1675 home 617-871-7613 w --- On Mon, 4/16/12, Elizabeth Chlipala wrote: From: Elizabeth Chlipala Subject: RE: [Histonet] Re: IHC in porcine tissues To: "'Galina Deyneko'" , "histonet@lists.utsouthwestern.edu" Date: Monday, April 16, 2012, 3:10 PM Galina Try MAC387 that is a macrophage/monocyte marker that cross reacts in porcine.? We use the one from abcam.? We have found that some of the anti-mouse polymer reagents cross react to porcine so we use a rabbit anti-mouse from Dako E0464 as a secondary and then an envision rabbit or strept avidin and that works much better. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galina Deyneko Sent: Monday, April 16, 2012 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: IHC in porcine tissues Dear Colleagues What? secondary antibody or polymer would be better to use for mouse primary antibody on FFPE? porcine tissues?Also, please share your experience regarding macrophages detection in porcine arteries. I found couple in Abcam and Serotec (only for frozen), but your opinions is very valuable. Thank you Galina Deyneko Novartis, Cambridge, MA 617-782-1675 home 617-871-7613 w _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anna.c.hughes <@t> gsk.com Tue Apr 17 12:51:58 2012 From: anna.c.hughes <@t> gsk.com (Anna Hughes) Date: Tue Apr 17 12:52:10 2012 Subject: [Histonet] Lectins that work with NHP vasculature Message-ID: <56B8D7633148A9419B559EBE8E9CF5903E87EF@019-SN1MPN1-033.019D.MGD.MSFT.NET> Hi Everyone! I was wondering if anyone knows of a lectin that will work to stain the retinal vasculature of NHP or Human tissue. Also, is there a digestion or permeability protocol that needs to be followed prior to staining the floating retina before flat mounting(EDTA, triton X...protease K)? Thanks in advance for your help! Anna C. Hughes GlaxoSmithKline 709 Swedeland Rd King of Prussia, PA 19406 anna.c.hughes@gsk.com From porathamanda <@t> gmail.com Tue Apr 17 13:51:33 2012 From: porathamanda <@t> gmail.com (amanda porath) Date: Tue Apr 17 13:51:39 2012 Subject: [Histonet] Smoothelin Antibody Message-ID: Is any one using "smoothelin" on the Leica Bond Max? Unfortunately I am having difficulty optimizing this antibody. If any one has any experience with it I would welcome their feedback. Thanks, Amanda Porath Bassett Medical Center Cooperstown, NY From melissa <@t> alliedsearchpartners.com Tue Apr 17 14:01:36 2012 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Tue Apr 17 14:01:55 2012 Subject: [Histonet] Immunohistochemistry Specialist Job in Texas Message-ID: Allied Search Partners has been retained in the search for a qualified IHC Technician/Technologist for a position in East Texas. The candidate should be a highly professional, client/patient-focused individual for a challenging and rewarding full-time career. Work in an environment where leading-edge instrumentation, methodology and information systems help deliver exceptional care to our patients. Position: Immunohistochemistry Technician/Technologist (histology) Schedule: Full time/day shift (Permanent/Direct Hire) Location: East Texas To Apply: Please email or fax resume to Melissa@alliedsearchpartners.com or fax to 888 388 7572. No other information is given about location or the organization at this time. Please send resume for review by our recruiters and all qualified candidates will be given a phone call to discuss further. Thank you! Summary: Performs standard Histology procedures including tissue processing, embedding, sectioning, and staining of paraffin embedded tissue. Histotechnician HT/HTL (ASCP)with strong experience in immunohistochemistry (IHC), qualifications in Immunohistochemistry (QIHC) desired. Must understand theories and principles involved in the performance of all histology procedures and immunoperoxidase stains. Ablitlity to prioritize duties and attentiveness to detail required. Computer proficiency required. Requirements: HT (ASCP) or HTL (ASCP) High School graduate 5 years technical with 1-2 years in special and immunostaining techniques 1 year School of Histotechnology or 1 year on-the-job training in large volume Anatomic Pathology Lab Lab Computer Skills: PC, Microsoft Word and Excel Lab Equipment: HE strainers Immunostainers Special strainers Additional Skills: Verbal, Personnel, Clerical, and Time Management Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com From melissa <@t> alliedsearchpartners.com Tue Apr 17 14:11:19 2012 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Tue Apr 17 14:11:32 2012 Subject: [Histonet] Part Time Histotech Job in Oregon (South of Vancouver, WA) Message-ID: Allied Search Partners, the leader in Histology recruitment is currently looking for a Part Time Histotech to work in a laboratory just South of Portland, OR. To Apply: Please submit resume by faxing or emailing to 888 388 7572 or melissa@alliedsearchpartners.com. The hours are flexible but this person will work 4-5 days per week for 20 hours per week, so please send also your availability for which days and hours you can possibly work. Location: South of Portland, OR and Vancouver, WA Schedule: Part Time. 20 Hours Per Week. 4-5 Days per week. Please submit your days and hours of availability during the weekdays with your resume. Also, please note that this candidate will work full time when the current full time histotech is on vacation. Please keep that in mind when applying for this position. Summary: Gross Dissection Microwave Processing Embedding Microtomy Automated Staining/Coverslipping Preparing Slides/Blocks for Shipping Requirements: HT ASCP Required HTL ASCP Preferred Some college coursework completed Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com From araniqkslvr <@t> yahoo.com Tue Apr 17 14:33:04 2012 From: araniqkslvr <@t> yahoo.com (Paula) Date: Tue Apr 17 14:33:11 2012 Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 22 Message-ID: <1334691184.8323.YahooMailClassic@web160205.mail.bf1.yahoo.com> I've recently been turned down for one histology position and TWO histology assistant positions. I stressed for the assistant positions that I felt it would be a great way to get back into the field (I last did this work in late 80s, except for a couple of weeks in 2007). I am also an older worker. I see ads that want the following qualifications: This is an award winning modern facility. Competitive salary and excellent benefit package. Must be able to demonstrate competency in a Histological Laboratory. Ability to perform routine histopathological tests. BS/BA MT Preferred however AS/AA MLT accepted. (Must be eligible for ASCP.) Medical Technologist ??? Job Code: 45618 ? TRS is seeking a skilled MT for this 13 week Histology assignment to start 03/21/2012. I have an A.A. in histotechnology, a B.S. in liberal arts, and passed the registry exam in late 80s, so it is still valid. I don't ask for a lot of pay. I've worked for a lot less than for what I assume a tech with experience would make, and that is ok with me as long as I can learn. I am ready to buy the books to study and learn again. I can't even get hired on as a $10 an hour specimen accsessioner. I have years of data entry experience but it seems I am not right for this either. I know there are a lot of people looking. I don't know what to do. Maybe if an assistant position comes open again near me I should not say I have my HT. Any suggestions? Besides getting younger--that I cannot do. Everytime I hear that techs are in demand I just want to barf. I know I don't have recent experience, but I'm willing to start at the bottom and re-learn. Paula From NKonop <@t> chw.org Tue Apr 17 14:37:09 2012 From: NKonop <@t> chw.org (Konop, Nicole) Date: Tue Apr 17 14:37:15 2012 Subject: [Histonet] Unregistered HT testing Message-ID: Hello everyone! I'm just curious to know if anyone is allowing unregistered HT's to do special stains in their CAP accredited lab? I have been involved in discussions regarding high complexity testing. From the feedback I have received, special stains and IHC stains are considered high complexity testing. I beg to disagree. I can understand IHC/ISH as high complexity but I don't think routine special stains fall under that category. I'd appreciate any feedback or literature you can reference for me to review. Thank you! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department From jaylundgren <@t> gmail.com Tue Apr 17 17:07:00 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Apr 17 17:07:09 2012 Subject: [Histonet] Unregistered HT testing In-Reply-To: References: Message-ID: It's a lot easier/cheaper for reproducibility, HR, and compliance to just buy an autostainer to do your special stains. Trying to do them by hand with unregistered techs is a bad idea. They are called "special" ;) for a reason. Buy a "special" ;) stainer, have the "special"ist ;) from the stainer company set it up to the Pathologist's specifications. Have your unregistered techs change the reagents, take the slides off the machines, and check the boxes on the logs. Your Pathologist, HR, the Federal Government, and the Insurance Companies will thank you. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Tue Apr 17 17:44:45 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Apr 17 17:44:59 2012 Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 22 In-Reply-To: <1334691184.8323.YahooMailClassic@web160205.mail.bf1.yahoo.com> References: <1334691184.8323.YahooMailClassic@web160205.mail.bf1.yahoo.com> Message-ID: <0F0B9B6B-1EB4-418F-AC70-A6F2E6C05A3D@yahoo.com> First off. Let me say I feel for you. You seem desperate to get a job and I hope you find one. Now, you can't not be hired because of age. Theres laws against that. And yes you should tell them your qualifications and be honest like you have been just now with this post. My suggestion is to seek shifts other people don't want in order to get your foot in the door to prove yourself. When you go for a job interview don't seem desperate too much. Have some confidence. Confidence creates confidence that you can handle the job. And what ever you do. Don't go to an interview and start telling them a bunch of negative things about your self. If you must say something that is negative think ahead of time for positive things to say about yourself. Write them down if that helps. Also think of mini stories to give them when they ask open ended questions like " give us a example when you had to deal with a irate coworker and how did you handle it" or another question might be " tell us of a mistake you made and how did you fix it and what did you do to prevent future errors". These are just examples of how many lab managers interview. Always put in tour answers a positive outcome and how you made it positive. I hope this helps you. I'd be glad to talk to you in private if you want someone to bounce ideas off of Kim D Sent from my iPhone On Apr 17, 2012, at 3:33 PM, Paula wrote: > > I've recently been turned down for one histology position and TWO > histology assistant positions. I stressed for the assistant positions > that I felt it would be a great way to get back into the field (I last > did this work in late 80s, except for a couple of weeks in 2007). I am > also an older worker. > > I see ads that want the following qualifications: > > This > is an award winning modern facility. Competitive salary and excellent > benefit package. Must be able to demonstrate competency in a > Histological Laboratory. Ability to perform routine histopathological > tests. BS/BA MT Preferred however AS/AA MLT accepted. (Must be eligible > for ASCP.) > > Medical Technologist > > Job Code: 45618 > > TRS is seeking a skilled MT for this 13 week Histology assignment to start 03/21/2012. > > I have an A.A. in histotechnology, a B.S. > in liberal arts, and passed the registry exam in late 80s, so it is still valid. > > I > don't ask for a lot of pay. I've worked for a lot less than for what I > assume a tech with experience would make, and that is ok with me as long > as I can learn. I am ready to buy the books to study and learn again. I > can't even get hired on as a $10 an hour specimen accsessioner. I have > years of data entry experience but it seems I am not right for this > either. > > I know there are a lot of people looking. I don't know > what to do. Maybe if an assistant position comes open again near me I > should not say I have my HT. > > Any suggestions? Besides getting younger--that I cannot do. > > Everytime > I hear that techs are in demand I just want to barf. I know I don't > have recent experience, but I'm willing to start at the bottom and > re-learn. > > Paula > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From susan <@t> labpath360.com Tue Apr 17 18:00:18 2012 From: susan <@t> labpath360.com (Susan Dowling) Date: Tue Apr 17 18:00:43 2012 Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 22 In-Reply-To: <0F0B9B6B-1EB4-418F-AC70-A6F2E6C05A3D@yahoo.com> References: <1334691184.8323.YahooMailClassic@web160205.mail.bf1.yahoo.com> <0F0B9B6B-1EB4-418F-AC70-A6F2E6C05A3D@yahoo.com> Message-ID: <835B6784-B3A8-45BC-AFFD-83BAA88E73B8@labpath360.com> Hi! If you need help Paula I will be happy to assist. I recruit for a lot of nice groups and know a lot of people! Susan Dowling, Owner Labpath360,LLC Cell; 864-915-2698 Office: 964-236-5095 On Apr 17, 2012, at 6:44 PM, Kim Donadio wrote: > First off. Let me say I feel for you. You seem desperate to get a job and I hope you find one. Now, you can't not be hired because of age. Theres laws against that. And yes you should tell them your qualifications and be honest like you have been just now with this post. My suggestion is to seek shifts other people don't want in order to get your foot in the door to prove yourself. When you go for a job interview don't seem desperate too much. Have some confidence. Confidence creates confidence that you can handle the job. And what ever you do. Don't go to an interview and start telling them a bunch of negative things about your self. If you must say something that is negative think ahead of time for positive things to say about yourself. Write them down if that helps. Also think of mini stories to give them when they ask open ended questions like " give us a example when you had to deal with a irate coworker and how did you handle it" or another question might be " tell us of a mistake you made and how did you fix it and what did you do to prevent future errors". These are just examples of how many lab managers interview. > > Always put in tour answers a positive outcome and how you made it positive. > > I hope this helps you. I'd be glad to talk to you in private if you want someone to bounce ideas off of > > Kim D > > Sent from my iPhone > > On Apr 17, 2012, at 3:33 PM, Paula wrote: > >> >> I've recently been turned down for one histology position and TWO >> histology assistant positions. I stressed for the assistant positions >> that I felt it would be a great way to get back into the field (I last >> did this work in late 80s, except for a couple of weeks in 2007). I am >> also an older worker. >> >> I see ads that want the following qualifications: >> >> This >> is an award winning modern facility. Competitive salary and excellent >> benefit package. Must be able to demonstrate competency in a >> Histological Laboratory. Ability to perform routine histopathological >> tests. BS/BA MT Preferred however AS/AA MLT accepted. (Must be eligible >> for ASCP.) >> >> Medical Technologist >> >> Job Code: 45618 >> >> TRS is seeking a skilled MT for this 13 week Histology assignment to start 03/21/2012. >> >> I have an A.A. in histotechnology, a B.S. >> in liberal arts, and passed the registry exam in late 80s, so it is still valid. >> >> I >> don't ask for a lot of pay. I've worked for a lot less than for what I >> assume a tech with experience would make, and that is ok with me as long >> as I can learn. I am ready to buy the books to study and learn again. I >> can't even get hired on as a $10 an hour specimen accsessioner. I have >> years of data entry experience but it seems I am not right for this >> either. >> >> I know there are a lot of people looking. I don't know >> what to do. Maybe if an assistant position comes open again near me I >> should not say I have my HT. >> >> Any suggestions? Besides getting younger--that I cannot do. >> >> Everytime >> I hear that techs are in demand I just want to barf. I know I don't >> have recent experience, but I'm willing to start at the bottom and >> re-learn. >> >> Paula >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Tue Apr 17 18:02:02 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Apr 17 18:02:13 2012 Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 22 In-Reply-To: <835B6784-B3A8-45BC-AFFD-83BAA88E73B8@labpath360.com> References: <1334691184.8323.YahooMailClassic@web160205.mail.bf1.yahoo.com> <0F0B9B6B-1EB4-418F-AC70-A6F2E6C05A3D@yahoo.com> <835B6784-B3A8-45BC-AFFD-83BAA88E73B8@labpath360.com> Message-ID: <187503C0-F882-4198-B068-9555349054CF@yahoo.com> There ya go Susan. Help this nice lady out. :) Sent from my iPhone On Apr 17, 2012, at 7:00 PM, Susan Dowling wrote: > Hi! If you need help Paula I will be happy to assist. I recruit for a lot of nice groups and know a lot of people! > > > > Susan Dowling, Owner > Labpath360,LLC > Cell; 864-915-2698 > Office: 964-236-5095 > > On Apr 17, 2012, at 6:44 PM, Kim Donadio wrote: > >> First off. Let me say I feel for you. You seem desperate to get a job and I hope you find one. Now, you can't not be hired because of age. Theres laws against that. And yes you should tell them your qualifications and be honest like you have been just now with this post. My suggestion is to seek shifts other people don't want in order to get your foot in the door to prove yourself. When you go for a job interview don't seem desperate too much. Have some confidence. Confidence creates confidence that you can handle the job. And what ever you do. Don't go to an interview and start telling them a bunch of negative things about your self. If you must say something that is negative think ahead of time for positive things to say about yourself. Write them down if that helps. Also think of mini stories to give them when they ask open ended questions like " give us a example when you had to deal with a irate coworker and how did you handle it" or another question might be " tell us of a mistake you made and how did you fix it and what did you do to prevent future errors". These are just examples of how many lab managers interview. >> >> Always put in tour answers a positive outcome and how you made it positive. >> >> I hope this helps you. I'd be glad to talk to you in private if you want someone to bounce ideas off of >> >> Kim D >> >> Sent from my iPhone >> >> On Apr 17, 2012, at 3:33 PM, Paula wrote: >> >>> >>> I've recently been turned down for one histology position and TWO >>> histology assistant positions. I stressed for the assistant positions >>> that I felt it would be a great way to get back into the field (I last >>> did this work in late 80s, except for a couple of weeks in 2007). I am >>> also an older worker. >>> >>> I see ads that want the following qualifications: >>> >>> This >>> is an award winning modern facility. Competitive salary and excellent >>> benefit package. Must be able to demonstrate competency in a >>> Histological Laboratory. Ability to perform routine histopathological >>> tests. BS/BA MT Preferred however AS/AA MLT accepted. (Must be eligible >>> for ASCP.) >>> >>> Medical Technologist >>> >>> Job Code: 45618 >>> >>> TRS is seeking a skilled MT for this 13 week Histology assignment to start 03/21/2012. >>> >>> I have an A.A. in histotechnology, a B.S. >>> in liberal arts, and passed the registry exam in late 80s, so it is still valid. >>> >>> I >>> don't ask for a lot of pay. I've worked for a lot less than for what I >>> assume a tech with experience would make, and that is ok with me as long >>> as I can learn. I am ready to buy the books to study and learn again. I >>> can't even get hired on as a $10 an hour specimen accsessioner. I have >>> years of data entry experience but it seems I am not right for this >>> either. >>> >>> I know there are a lot of people looking. I don't know >>> what to do. Maybe if an assistant position comes open again near me I >>> should not say I have my HT. >>> >>> Any suggestions? Besides getting younger--that I cannot do. >>> >>> Everytime >>> I hear that techs are in demand I just want to barf. I know I don't >>> have recent experience, but I'm willing to start at the bottom and >>> re-learn. >>> >>> Paula >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Tue Apr 17 18:43:21 2012 From: histotalk <@t> yahoo.com (David Kemler) Date: Tue Apr 17 18:43:28 2012 Subject: [Histonet] Thanks everyone! Message-ID: <1334706201.72856.YahooMailNeo@web120605.mail.ne1.yahoo.com> Thank you all who joined me?for the Region III Meeting?at Callaway Gardens, Pine Mountain, GA?hosted by the Georgia Society for Histotechnology?this past weekend. The HistoTALK "mobile studio" always had someone stopping by to say hello. Lots of fun! ? The interviews that were done at the Region III will begin airing this Sunday, April 22nd. So be sure to log on at www.HistoTALK.com. Our guest will be Jack Ratiff and it was a way cool interview you will agree. Somehow when Jack is on the show, it nearly?becomes "Wild Kingdom". ? A special thanks goes out to Wanda Simons, Mike Ayers and?the whole GSH ?BOD for inviting me. ? Yours, David ? BTW, HistoTALK was invited to be part of the Region VII Meeting in Phoenix, AZ on June 8 - 10. If you would like to be a guest on the show, just let me know and I'll plug you in. From foreightl <@t> gmail.com Tue Apr 17 18:43:43 2012 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Apr 17 18:43:50 2012 Subject: [Histonet] Hirschsprung's protocol Message-ID: Hello, I was wondering if anyone could share with me their lab's Hirschsprung's protocol. We want to do an good job with the number of required sections/levels, however we want to ensure that it isn't overly onerous on the histotechnicians. Thanks, -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From conniegrubaugh <@t> hotmail.com Tue Apr 17 19:23:55 2012 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Tue Apr 17 19:23:59 2012 Subject: [Histonet] KNOWNPROTOCAL Message-ID: I came in late on this discussion but we use this system and out Medical Director loves it and so does our clients. We have used this for other than just prostate. We have used it for breasts, skin bx, and gastric bx's so far. KnownProtocal takes care of all the paper work the billing and reimberses out lab for the time it takes us to collect the specimen and send it to them. So do not be so negative and you may just need their protocal some time. Connie G. From JMacDonald <@t> mtsac.edu Tue Apr 17 22:26:51 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Apr 17 22:27:54 2012 Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 22 In-Reply-To: <1334691184.8323.YahooMailClassic@web160205.mail.bf1.yahoo.com> Message-ID: Are you MT or MLT certified? These are certifications from the ASCP and the ad is looking for someone that is certified or eligible. Paula Sent by: histonet-bounces@lists.utsouthwestern.edu 04/17/2012 12:34 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Re: Histonet Digest, Vol 101, Issue 22 I've recently been turned down for one histology position and TWO histology assistant positions. I stressed for the assistant positions that I felt it would be a great way to get back into the field (I last did this work in late 80s, except for a couple of weeks in 2007). I am also an older worker. I see ads that want the following qualifications: This is an award winning modern facility. Competitive salary and excellent benefit package. Must be able to demonstrate competency in a Histological Laboratory. Ability to perform routine histopathological tests. BS/BA MT Preferred however AS/AA MLT accepted. (Must be eligible for ASCP.) Medical Technologist Job Code: 45618 TRS is seeking a skilled MT for this 13 week Histology assignment to start 03/21/2012. I have an A.A. in histotechnology, a B.S. in liberal arts, and passed the registry exam in late 80s, so it is still valid. I don't ask for a lot of pay. I've worked for a lot less than for what I assume a tech with experience would make, and that is ok with me as long as I can learn. I am ready to buy the books to study and learn again. I can't even get hired on as a $10 an hour specimen accsessioner. I have years of data entry experience but it seems I am not right for this either. I know there are a lot of people looking. I don't know what to do. Maybe if an assistant position comes open again near me I should not say I have my HT. Any suggestions? Besides getting younger--that I cannot do. Everytime I hear that techs are in demand I just want to barf. I know I don't have recent experience, but I'm willing to start at the bottom and re-learn. Paula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Apr 17 22:51:17 2012 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Apr 17 22:52:16 2012 Subject: [Histonet] California Society Annual Symposium Message-ID: ?Come Ride the Waves of Innovation? This year?s meeting will be at the Bahia resort hotel on Mission Bay. 2 Blocks from the Ocean. We have an outstanding list of lecturers. Classes will be held on 2 paddle wheel boats. Take a look at the hotel website. Details and registration are posted on the Society website. http://www.bahiahotel.com/ http://www.californiahistology.org/events.html The 2012 Symposium/Convention will be held in May in San Diego, CA. When: May 4 - 6, 2012 Hotel Information: Bahia Resort Hotel 998 West Mission Bay Drive, San Diego CA 858.488.0551 Reserve your room now at: https://shop.evanshotels.com/bahia_groups/casocf1205093.html Note: To receive the group rate of $109.00 per night please mention CSH when registering with the hotel. The cutoff date for the group rate is April 3, 2012. We have already met our room number minimum, but more rooms are available at the discount rate. Register and book you room soon. Kathy Hardy, HT (ASCP), LATG, President CSH James Watson HT (ASCP), vice President CSH From member <@t> linkedin.com Wed Apr 18 08:20:11 2012 From: member <@t> linkedin.com (erskine husbands via LinkedIn) Date: Wed Apr 18 08:20:19 2012 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <21086237.11506611.1334755211982.JavaMail.app@ela4-app0129.prod> LinkedIn ------------ erskine husbands requested to add you as a connection on LinkedIn: ------------------------------------------ David, I'd like to add you to my professional network on LinkedIn. - erskine Accept invitation from erskine husbands http://www.linkedin.com/e/yvpgd1-h16esgy2-1j/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I254141263_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYPdz8Nd34Qdj99bSVQckF3iD9UbP0Rd3sVdPsPcPoLrCBxbOYWrSlI/EML_comm_afe/?hs=false&tok=1ic6cAFAYwsBc1 View invitation from erskine husbands http://www.linkedin.com/e/yvpgd1-h16esgy2-1j/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I254141263_13/3cNnPcScz4QcjgRcAALqnpPbOYWrSlI/svi/?hs=false&tok=2fk2GPPfswsBc1 ------------------------------------------ Why might connecting with erskine husbands be a good idea? erskine husbands's connections could be useful to you: After accepting erskine husbands's invitation, check erskine husbands's connections to see who else you may know and who you might want an introduction to. Building these connections can create opportunities in the future. -- (c) 2012, LinkedIn Corporation From ASelf <@t> georgetownhospitalsystem.org Wed Apr 18 08:55:55 2012 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Wed Apr 18 08:56:25 2012 Subject: [Histonet] Batch Controls Message-ID: Good Morning and Happy Wednesday to everyone, For those of you that are running batch controls with your IHC stains I have a couple of questions for you. What is your process for review and documentation of the batch control slide? How do you label/identify this slide for easy retrieval? (Do you identify this slide by date run or stain run) Thanks in advance for everyone's help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From CThornton <@t> dahlchase.com Wed Apr 18 09:04:35 2012 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Wed Apr 18 09:04:45 2012 Subject: [Histonet] PAX-8 antibody Message-ID: Looking for help with a protocol for this antibody using Ventana platform, either Ultra or Benchmark, Ultraview detection and Biocare's predilute antibody. Would like protocol for any other vendor's antibody as well. We are having issues with light staining and too much background. thank you! Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From lblazek <@t> digestivespecialists.com Wed Apr 18 09:25:41 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Apr 18 09:25:51 2012 Subject: [Histonet] RE: Batch Controls In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E3913819D4419@IBMB7Exchange.digestivespecialists.com> Amy, We have a log that we document the control results on and the run number of the control. The batch control slides are filed by date. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Wednesday, April 18, 2012 9:56 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Batch Controls Good Morning and Happy Wednesday to everyone, For those of you that are running batch controls with your IHC stains I have a couple of questions for you. What is your process for review and documentation of the batch control slide? How do you label/identify this slide for easy retrieval? (Do you identify this slide by date run or stain run) Thanks in advance for everyone's help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjdessoye <@t> commonwealthhealth.net Wed Apr 18 09:43:22 2012 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Wed Apr 18 09:43:31 2012 Subject: [Histonet] Unregistered HT testing In-Reply-To: Message-ID: Our interpretation of special stains/IHC/ISH is that it is the 'interpretation' of these stains that is 'high complexity'. I believe that CLIA is the place where 'high complexity' is defined (although CAP may expand on this) and CLIA does not recognize ASCP registration. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Konop, Nicole [mailto:NKonop@chw.org] Sent: Tuesday, April 17, 2012 3:37 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unregistered HT testing Hello everyone! I'm just curious to know if anyone is allowing unregistered HT's to do special stains in their CAP accredited lab? I have been involved in discussions regarding high complexity testing. From the feedback I have received, special stains and IHC stains are considered high complexity testing. I beg to disagree. I can understand IHC/ISH as high complexity but I don't think routine special stains fall under that category. I'd appreciate any feedback or literature you can reference for me to review. Thank you! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From jessica <@t> prometheushealthcare.com Wed Apr 18 09:43:34 2012 From: jessica <@t> prometheushealthcare.com (Jessica-Prometheus) Date: Wed Apr 18 09:43:52 2012 Subject: [Histonet] Histotechnician Position in Ohio! Message-ID: <01e001cd1d71$a212e670$e638b350$@prometheushealthcare.com> Hey everyone, I specialize in the recruitment of lab professionals in Ohio, and I'm looking to fill a full time, day time Histotechnician position at a leading healthcare provider in Zanesville, OH. The hours are Monday - Friday with flexible start times, and there are no weekends or on call hours. The ideal candidate would be HT-ASCP and be proficient with Tissue Processors, Micrometers, Immunohistochemical and Special Staining Instrumentation. Recent grads are encouraged to apply, but preference will be given to experienced candidates. The company offers a very competitive salary, full benefits, and relocation is negotiable. If you are interested or know anyone who might be, please contact me. Please feel free to pass along this information as well. For immediate consideration, just email me your most up to date resume. Thank you! Jessica Sanchez Account Manager Prometheus Healthcare Office 301-693-9057 Direct 301-693-8908 Fax 301-368-2478 jessica@prometheushealthcare.com www.prometheushealthcare.com http://twitter.com/PrometheusBlog Click Here to Meet Me! From fpearsa <@t> clemson.edu Wed Apr 18 10:31:15 2012 From: fpearsa <@t> clemson.edu (Fran Pearsall) Date: Wed Apr 18 10:31:22 2012 Subject: [Histonet] Steiner stain Message-ID: <5.0.0.25.2.20120418112138.00bc6600@mail.clemson.edu> Hi Everyone, The counter stain faded on my slides. I've never had that happen before, but they were left overnight in zylene before being cover-slipped. Can I go back and remove the cover slip, which step would I back up to regain the staining? Or, do I need to completely start the stain again? We use the micro-wave method using a box kit from Sigma, the Accumate H2 100 . I wonder if I need to re-make the reducing solution, I was trying to see if I can save using another hydroquinone tablet? Thanks From rjbuesa <@t> yahoo.com Wed Apr 18 11:57:07 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 18 11:57:14 2012 Subject: [Histonet] Steiner stain In-Reply-To: <5.0.0.25.2.20120418112138.00bc6600@mail.clemson.edu> Message-ID: <1334768227.72470.YahooMailClassic@web162101.mail.bf1.yahoo.com> I imagine that you also ran a (+) control. If in the (+) control the organisms are well stained, just remove the coverslip, counterstain again and cover again. Ren? J. --- On Wed, 4/18/12, Fran Pearsall wrote: From: Fran Pearsall Subject: [Histonet] Steiner stain To: histonet@lists.utsouthwestern.edu Date: Wednesday, April 18, 2012, 11:31 AM Hi? Everyone, The counter stain faded on my slides.? I've never had that happen before, but they were left overnight in zylene before being cover-slipped.? Can I go back and remove the cover slip,? which step would I back up to regain the staining?? Or, do I need to completely start the stain again?? We use the micro-wave method using a box kit from Sigma, the Accumate H2 100 .? I wonder if I need to re-make? the reducing solution, I was trying to see if I can save using another hydroquinone tablet? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Apr 18 11:59:16 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 18 11:59:23 2012 Subject: [Histonet] Batch Controls In-Reply-To: Message-ID: <1334768356.79827.YahooMailClassic@web162105.mail.bf1.yahoo.com> I used to date the batch control and file it along with other batch controls. The pathologists reviewed the batch control for quality. They were filed by dates. Ren? j. --- On Wed, 4/18/12, Amy Self wrote: From: Amy Self Subject: [Histonet] Batch Controls To: "'histonet@lists.utsouthwestern.edu'" Date: Wednesday, April 18, 2012, 9:55 AM Good Morning and Happy Wednesday to everyone, For those of you that are running batch controls with your IHC stains I have a couple of questions for you. What is your process for review and documentation of the batch control slide? How do you label/identify this slide for easy retrieval? (Do you identify this slide by date run or stain run) Thanks in advance for everyone's help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Apr 18 12:17:08 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Apr 18 12:17:18 2012 Subject: [Histonet] Unregistered HT testing In-Reply-To: References: , Message-ID: CLIA does stipulate education requirements though for high complexity( I don't have it handy, but I do recall it is quite specific).Yes "interpretation" differs from performance. But we are not interpreting for diagnosis anyhow, but we do have to make technical assessments, corrections, and I think you need some insight to do that effectively. As I recall from CLIA, IHC/ISH is clearly under high complexity. But I can think of some histochemical staining ( such as enzyme muscle histochemistry) that might also be considered high complexity. I know for grossing, it is also an issue. Even if ASCP certification is not specifically stipulated in CLIA , I think that is is implied , in that the education stipulations are roughly equivalent to the criteria for HT certification- (associates with qtr. hours needed in science, chemistry etc). So yes I guess anyone can learn to put on and take off slides on a stainer and execute any process or procedure when trained to do so. The "rub" for me though is that I personally have observed that when people don't have training or education in histology ( formal or informal), they don't know how the stain works, or what it should show, so they are not adept at review of stained slides, and are not able to troubleshoot stain problems etc. I think that this should be part of the expectation, but that is an opinion, not I guess actually regulated as a requirement. I do think that CAP pretty much stipulates this expectation via competency requirements, but that is how I interpret it. Certification is just a means to communicate that you have satisfied the criteria, which should say that you have this underlying knowledge and understanding- knowing science just forms the basis for this understanding. I think good in house laboratory training and competency assessment could be an alternate route to that goal in places where more academic training opportunities are scarce. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 18 Apr 2012 10:43:22 -0400 > From: mjdessoye@commonwealthhealth.net > To: NKonop@chw.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT testing > CC: > > Our interpretation of special stains/IHC/ISH is that it is the > 'interpretation' of these stains that is 'high complexity'. I believe > that CLIA is the place where 'high complexity' is defined (although CAP > may expand on this) and CLIA does not recognize ASCP registration. > > Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General > Hospital | An Affiliate of Commonwealth Health | > mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, > PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 > > > -----Original Message----- > From: Konop, Nicole [mailto:NKonop@chw.org] > Sent: Tuesday, April 17, 2012 3:37 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Unregistered HT testing > > Hello everyone! > > I'm just curious to know if anyone is allowing unregistered HT's to do > special stains in their CAP accredited lab? I have been involved in > discussions regarding high complexity testing. From the feedback I have > received, special stains and IHC stains are considered high complexity > testing. I beg to disagree. I can understand IHC/ISH as high > complexity but I don't think routine special stains fall under that > category. I'd appreciate any feedback or literature you can reference > for me to review. Thank you! > > Nicole Anne Konop BS, HTL(ASCP) > Histology Team Lead > Children's Hospital of Wisconsin > (414)266-6580 Direct Line > (414)907-0366 Pager > (414)266-2524 Histology Department > > > > > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Commonwealth Health. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Wed Apr 18 12:22:10 2012 From: b427297 <@t> aol.com (b427297@aol.com) Date: Wed Apr 18 12:22:44 2012 Subject: [Histonet] Unregistered HT testing In-Reply-To: References: Message-ID: The pathologist is interpreting the staining result, right? The HT is only evaluating the stain quality, not the results. Sent from my iPhone On Apr 18, 2012, at 9:43 AM, "Dessoye, Michael J" wrote: > Our interpretation of special stains/IHC/ISH is that it is the > 'interpretation' of these stains that is 'high complexity'. I believe > that CLIA is the place where 'high complexity' is defined (although CAP > may expand on this) and CLIA does not recognize ASCP registration. > > Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General > Hospital | An Affiliate of Commonwealth Health | > mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, > PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 > > > -----Original Message----- > From: Konop, Nicole [mailto:NKonop@chw.org] > Sent: Tuesday, April 17, 2012 3:37 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Unregistered HT testing > > Hello everyone! > > I'm just curious to know if anyone is allowing unregistered HT's to do > special stains in their CAP accredited lab? I have been involved in > discussions regarding high complexity testing. From the feedback I have > received, special stains and IHC stains are considered high complexity > testing. I beg to disagree. I can understand IHC/ISH as high > complexity but I don't think routine special stains fall under that > category. I'd appreciate any feedback or literature you can reference > for me to review. Thank you! > > Nicole Anne Konop BS, HTL(ASCP) > Histology Team Lead > Children's Hospital of Wisconsin > (414)266-6580 Direct Line > (414)907-0366 Pager > (414)266-2524 Histology Department > > > > > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Commonwealth Health. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 1dpeterson <@t> meriter.com Wed Apr 18 12:33:14 2012 From: 1dpeterson <@t> meriter.com (Peterson, Dan) Date: Wed Apr 18 12:33:22 2012 Subject: [Histonet] Tissue Problem Message-ID: <466B666475DE6547BBB0641E540A4BB5115B4B75EC@EXVS1.meriter.com> OK netters, I've got a problem that's just cropped up over the last week or so. It's happening to our larger specimens, and only the ones containing tumor. When we cut a slide, the tissue looks processed well, but as the slide dries, the tissue area turns really white. Then when we try and stain it (especially Immunos) just that portion of the section falls off. Any clues to defeat this gremlin would be greatly appreciated. Dan Daniel R Peterson HT(ASCP) Histopathology Technical Specialist Meriter Laboratories (608) 417-6557 fax (608) 417-6343 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. From joelleweaver <@t> hotmail.com Wed Apr 18 12:34:07 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Apr 18 12:34:14 2012 Subject: [Histonet] Batch Controls In-Reply-To: <1334768356.79827.YahooMailClassic@web162105.mail.bf1.yahoo.com> References: , <1334768356.79827.YahooMailClassic@web162105.mail.bf1.yahoo.com> Message-ID: I have used the date method, so long as the batch control is run on the same run, same platform, retrieval and reaction conditions. When there was different retrieval or conditions/variables within one case, I used another representative control for the differences . I like the controls on the same slide as at least one section from the block/case that is batched. But I am told that I am too strict with my method preferences and "fussy". My supervisor thinks I am a real pain ! You have to date/mark the patient slides in the same way then so that if you have to pull the case for legal, consult or inspection you can associate them from the ever-expanding files. I like to keep the test controls and batch controls basically forever. I know that is a storage issue, but I have had to pull cases from way back with controls both +/-, so I don't like that to be a big problem. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 18 Apr 2012 09:59:16 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; ASelf@georgetownhospitalsystem.org > Subject: Re: [Histonet] Batch Controls > CC: > > I used to date the batch control and file it along with other batch controls. The pathologists reviewed the batch control for quality. > They were filed by dates. > Ren? j. > > --- On Wed, 4/18/12, Amy Self wrote: > > > From: Amy Self > Subject: [Histonet] Batch Controls > To: "'histonet@lists.utsouthwestern.edu'" > Date: Wednesday, April 18, 2012, 9:55 AM > > > Good Morning and Happy Wednesday to everyone, > > For those of you that are running batch controls with your IHC stains I have a couple of questions for you. > > What is your process for review and documentation of the batch control slide? > > How do you label/identify this slide for easy retrieval? (Do you identify this slide by date run or stain run) > > > Thanks in advance for everyone's help, > > Amy > > Amy Self > Georgetown Hospital System > 843-527-7179 > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Apr 18 12:38:33 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Apr 18 12:38:41 2012 Subject: [Histonet] Tissue Problem In-Reply-To: <466B666475DE6547BBB0641E540A4BB5115B4B75EC@EXVS1.meriter.com> References: <466B666475DE6547BBB0641E540A4BB5115B4B75EC@EXVS1.meriter.com> Message-ID: Can you supply the processing instrument, method/protocol, is it limited to certain tissue types, and estimate of specimen dimensions? That would make it easier to take a stab. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: 1dpeterson@meriter.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 18 Apr 2012 12:33:14 -0500 > Subject: [Histonet] Tissue Problem > > OK netters, > I've got a problem that's just cropped up over the last week or so. > It's happening to our larger specimens, and only the ones containing tumor. > When we cut a slide, the tissue looks processed well, but as the slide dries, > the tissue area turns really white. Then when we try and stain it (especially Immunos) > just that portion of the section falls off. Any clues to defeat this gremlin would be greatly > appreciated. > > Dan > > Daniel R Peterson HT(ASCP) > Histopathology Technical Specialist > Meriter Laboratories > (608) 417-6557 > fax (608) 417-6343 > 1dpeterson@meriter.com > > CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjdessoye <@t> commonwealthhealth.net Wed Apr 18 13:06:55 2012 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Wed Apr 18 13:07:32 2012 Subject: [Histonet] Unregistered HT testing In-Reply-To: Message-ID: Just to be clear, I wholly agree with your comments...I am not downplaying registration by any means, and I think that IHC and ISH should fall under high complexity. I think this issue gets downplayed because in many cases one can 'get by' by putting slides on a stainer and running it, and that doesn't 'appear' to be high complexity...but without the background knowledge and understanding that you talked about, these folks can't troubleshoot issues when they come up or understand the principles behind the procedures. To get back to the original question, I do know labs that have this testing performed by unregistered techs...however, they all have degrees and on-the-job training, so I imagine most would fall into the 'registration eligible' category, and be qualified for high-complexity testing. In my opinion, that would meet the CAP requirement. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 ________________________________ From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Wednesday, April 18, 2012 1:17 PM To: Dessoye, Michael J; nkonop@chw.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT testing CLIA does stipulate education requirements though for high complexity( I don't have it handy, but I do recall it is quite specific). Yes "interpretation" differs from performance. But we are not interpreting for diagnosis anyhow, but we do have to make technical assessments, corrections, and I think you need some insight to do that effectively. As I recall from CLIA, IHC/ISH is clearly under high complexity. But I can think of some histochemical staining ( such as enzyme muscle histochemistry) that might also be considered high complexity. I know for grossing, it is also an issue. Even if ASCP certification is not specifically stipulated in CLIA , I think that is is implied , in that the education stipulations are roughly equivalent to the criteria for HT certification- (associates with qtr. hours needed in science, chemistry etc). So yes I guess anyone can learn to put on and take off slides on a stainer and execute any process or procedure when trained to do so. The "rub" for me though is that I personally have observed that when people don't have training or education in histology ( formal or informal), they don't know how the stain works, or what it should show, so they are not adept at review of stained slides, and are not able to troubleshoot stain problems etc. I think that this should be part of the expectation, but that is an opinion, not I guess actually regulated as a requirement. I do think that CAP pretty much stipulates this expectation via competency requirements, but that is how I interpret it. Certification is just a means to communicate that you have satisfied the criteria, which should say that you have this underlying knowledge and understanding- knowing science just forms the basis for this understanding. I think good in house laboratory training and competency assessment could be an alternate route to that goal in places where more academic training opportunities are scarce. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 18 Apr 2012 10:43:22 -0400 > From: mjdessoye@commonwealthhealth.net > To: NKonop@chw.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT testing > CC: > > Our interpretation of special stains/IHC/ISH is that it is the > 'interpretation' of these stains that is 'high complexity'. I believe > that CLIA is the place where 'high complexity' is defined (although CAP > may expand on this) and CLIA does not recognize ASCP registration. > > Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General > Hospital | An Affiliate of Commonwealth Health | > mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, > PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 > > > -----Original Message----- > From: Konop, Nicole [mailto:NKonop@chw.org] > Sent: Tuesday, April 17, 2012 3:37 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Unregistered HT testing > > Hello everyone! > > I'm just curious to know if anyone is allowing unregistered HT's to do > special stains in their CAP accredited lab? I have been involved in > discussions regarding high complexity testing. From the feedback I have > received, special stains and IHC stains are considered high complexity > testing. I beg to disagree. I can understand IHC/ISH as high > complexity but I don't think routine special stains fall under that > category. I'd appreciate any feedback or literature you can reference > for me to review. Thank you! > > Nicole Anne Konop BS, HTL(ASCP) > Histology Team Lead > Children's Hospital of Wisconsin > (414)266-6580 Direct Line > (414)907-0366 Pager > (414)266-2524 Histology Department > > > > > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Commonwealth Health. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From mjdessoye <@t> commonwealthhealth.net Wed Apr 18 13:13:03 2012 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Wed Apr 18 13:13:18 2012 Subject: [Histonet] Unregistered HT testing In-Reply-To: Message-ID: Yes, the pathologist is interpreting. Although the HT is evaluating the stain quality, I personally think that it should fall under 'high complexity testing'. To clear up what I was getting at, an unregistered tech can still meet the CLIA requirements for high complexity testing. If you have an unregistered tech performing the test, I think you should make sure that they are qualified under the CLIA requirements. -----Original Message----- From: b427297@aol.com [mailto:b427297@aol.com] Sent: Wednesday, April 18, 2012 1:22 PM To: Dessoye, Michael J Cc: Konop, Nicole; Subject: Re: [Histonet] Unregistered HT testing The pathologist is interpreting the staining result, right? The HT is only evaluating the stain quality, not the results. Sent from my iPhone On Apr 18, 2012, at 9:43 AM, "Dessoye, Michael J" wrote: > Our interpretation of special stains/IHC/ISH is that it is the > 'interpretation' of these stains that is 'high complexity'. I believe > that CLIA is the place where 'high complexity' is defined (although > CAP may expand on this) and CLIA does not recognize ASCP registration. > > Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General > Hospital | An Affiliate of Commonwealth Health | > mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, > PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 > > > -----Original Message----- > From: Konop, Nicole [mailto:NKonop@chw.org] > Sent: Tuesday, April 17, 2012 3:37 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Unregistered HT testing > > Hello everyone! > > I'm just curious to know if anyone is allowing unregistered HT's to do > special stains in their CAP accredited lab? I have been involved in > discussions regarding high complexity testing. From the feedback I > have received, special stains and IHC stains are considered high > complexity testing. I beg to disagree. I can understand IHC/ISH as > high complexity but I don't think routine special stains fall under > that category. I'd appreciate any feedback or literature you can > reference for me to review. Thank you! > > Nicole Anne Konop BS, HTL(ASCP) > Histology Team Lead > Children's Hospital of Wisconsin > (414)266-6580 Direct Line > (414)907-0366 Pager > (414)266-2524 Histology Department > > > > > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom they > are addressed. > If you have received this email in error please notify the originator > of the message. This footer also confirms that this email message has > been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, states > them to be the views of Commonwealth Health. > > Scanning of this message and addition of this footer is performed by > Websense Email Security software in conjunction with virus detection > software. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From joelleweaver <@t> hotmail.com Wed Apr 18 13:23:30 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Apr 18 13:23:38 2012 Subject: [Histonet] Unregistered HT testing In-Reply-To: References: , Message-ID: True, and I agree with your statement. Yes I also think that you could meet CLIA without certification if you meet the education requirements. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 18 Apr 2012 14:13:03 -0400 > From: mjdessoye@commonwealthhealth.net > To: b427297@aol.com > Subject: RE: [Histonet] Unregistered HT testing > CC: histonet@lists.utsouthwestern.edu; NKonop@chw.org > > Yes, the pathologist is interpreting. Although the HT is evaluating the > stain quality, I personally think that it should fall under 'high > complexity testing'. > > To clear up what I was getting at, an unregistered tech can still meet > the CLIA requirements for high complexity testing. If you have an > unregistered tech performing the test, I think you should make sure that > they are qualified under the CLIA requirements. > > -----Original Message----- > From: b427297@aol.com [mailto:b427297@aol.com] > Sent: Wednesday, April 18, 2012 1:22 PM > To: Dessoye, Michael J > Cc: Konop, Nicole; > Subject: Re: [Histonet] Unregistered HT testing > > The pathologist is interpreting the staining result, right? The HT is > only evaluating the stain quality, not the results. > > Sent from my iPhone > > On Apr 18, 2012, at 9:43 AM, "Dessoye, Michael J" > wrote: > > > Our interpretation of special stains/IHC/ISH is that it is the > > 'interpretation' of these stains that is 'high complexity'. I believe > > > that CLIA is the place where 'high complexity' is defined (although > > CAP may expand on this) and CLIA does not recognize ASCP registration. > > > > Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General > > > Hospital | An Affiliate of Commonwealth Health | > > mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, > > > PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 > > > > > > -----Original Message----- > > From: Konop, Nicole [mailto:NKonop@chw.org] > > Sent: Tuesday, April 17, 2012 3:37 PM > > To: 'histonet@lists.utsouthwestern.edu' > > Subject: [Histonet] Unregistered HT testing > > > > Hello everyone! > > > > I'm just curious to know if anyone is allowing unregistered HT's to do > > > special stains in their CAP accredited lab? I have been involved in > > discussions regarding high complexity testing. From the feedback I > > have received, special stains and IHC stains are considered high > > complexity testing. I beg to disagree. I can understand IHC/ISH as > > high complexity but I don't think routine special stains fall under > > that category. I'd appreciate any feedback or literature you can > > reference for me to review. Thank you! > > > > Nicole Anne Konop BS, HTL(ASCP) > > Histology Team Lead > > Children's Hospital of Wisconsin > > (414)266-6580 Direct Line > > (414)907-0366 Pager > > (414)266-2524 Histology Department > > > > > > > > > > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > _ _ _ > > > > This email and any files transmitted with it are confidential and > > intended solely for the use of the individual or entity to whom they > > are addressed. > > If you have received this email in error please notify the originator > > of the message. This footer also confirms that this email message has > > been scanned for the presence of computer viruses. > > > > Any views expressed in this message are those of the individual > > sender, except where the sender specifies and with authority, states > > them to be the views of Commonwealth Health. > > > > Scanning of this message and addition of this footer is performed by > > Websense Email Security software in conjunction with virus detection > > software. > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Commonwealth Health. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdboydhisto <@t> yahoo.com Wed Apr 18 13:31:25 2012 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Wed Apr 18 13:31:27 2012 Subject: [Histonet] LIS questions In-Reply-To: <1334687386.74294.YahooMailNeo@web125801.mail.ne1.yahoo.com> References: <1334687386.74294.YahooMailNeo@web125801.mail.ne1.yahoo.com> Message-ID: <1334773885.90033.YahooMailNeo@web125805.mail.ne1.yahoo.com> ? Is anyone out there familiar with any of the following LIS systems, if so, what are your thoughts??? AIM Path Software systems, Wavefront Software, WinSURGE, CSS LIS ? What are the opinions out there?for a server based system versus a cloud based?? Thanks! Kelly From talulahgosh <@t> gmail.com Wed Apr 18 13:31:52 2012 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Apr 18 13:31:54 2012 Subject: [Histonet] looking for "chuck" for old rmc cryostat Message-ID: Hello Histonetters! I am looking for a "chuck" (the metal disc you glue the block to) for an old RMC cryostat. Unlike newer cryostats, the piece that locks into the specimen head is a screw, not a thick base. Any idea where to get this? I haven't fully searched the internet but any help is appreciated. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson From 1dpeterson <@t> meriter.com Wed Apr 18 13:46:11 2012 From: 1dpeterson <@t> meriter.com (Peterson, Dan) Date: Wed Apr 18 13:46:19 2012 Subject: [Histonet] Tissue Problem part 2 Message-ID: <466B666475DE6547BBB0641E540A4BB5115B4B75F1@EXVS1.meriter.com> Hello again netters. As usually happens, trying to keep a message brief, leaves out all sorts of important info. Like, I've already completely changed the processors, and checked ph of reagents. We run the standard 8-10 hr program that almost all of us use. (Same program for 30 + yrs) It's only been on a few blocks. Unfortunately, it's the tumor in the blocks that's affected. Tissue type has been kidney, colon, and breast. 2 of the 3 tissue type sat all weekend, in cassettes, in formalin. Dan Daniel R Peterson HT(ASCP) Histopathology Technical Specialist Meriter Laboratories (608) 417-6557 fax (608) 417-6343 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. From carol.wilson <@t> ricerca.com Wed Apr 18 13:49:18 2012 From: carol.wilson <@t> ricerca.com (Wilson, Carol) Date: Wed Apr 18 13:50:01 2012 Subject: [Histonet] Cresyl Violet as a counterstain Message-ID: <19848F1C0886A5409C729CC2136EDAFF0591C53EA6@IAD2MBX09.mex02.mlsrvr.com> Hi All, Looking for suggestions or protocol for staining 20 micron rat brain sections with a Prussian Blue w/cresyl violet counterstain and a modified Wright Giemsa w/ Cresyl Violet counterstain. Any suggestions or tips to do this would be greatly appreciated. Both for dealing with these very thick sections and then also for the staining. Thanks in advance. Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC From karengary <@t> mplpath.com Wed Apr 18 14:26:08 2012 From: karengary <@t> mplpath.com (Karen Gary) Date: Wed Apr 18 14:25:35 2012 Subject: [Histonet] PIN4 Message-ID: <004701cd1d99$1b2984c0$517c8e40$@mplpath.com> I am trying to work up the PIN4 cocktail. Can anyone out there help me with a procedure? We use the Benckmark Ultra. I have the Ventana basal Cell Cocktail and the P504s from Cell Marque that I was told by my Ventana rep to order. All help is appreciated!! Karen S. Gary HT (ASCP), BBA Laboratory Manager Confidentiality Notice: The information contained in this transmission may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately notify me by telephone and permanently delete the original and any copy of this e-mail and destroy any printout thereof. From LStadler <@t> cbiolabs.com Wed Apr 18 14:35:07 2012 From: LStadler <@t> cbiolabs.com (Lyn Stadler) Date: Wed Apr 18 14:35:11 2012 Subject: [Histonet] Sakura VIP 3000 Tissue Processor Message-ID: <98CC14B915EBA84B9A326D45CC3C1DEC9A479905@cbiolabs05.CBiolabs.local> Anyone out there using a VIP 3000? I need some replacement gaskets that Sakuara no longer manufactures. Anyone know of an alternate vendor or supplier that carries them? Or, better yet, anyone have some that they are interested in giving away ;). Thanks in advance! This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From Rcartun <@t> harthosp.org Wed Apr 18 14:41:16 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Apr 18 14:41:28 2012 Subject: [Histonet] Batch Controls In-Reply-To: References: Message-ID: <4F8EE09C.7400.0077.1@harthosp.org> As the laboratory Director, I can sign-off on the positive control slides or my staff can do it since I have delegated this responsibility to them. We all use a different color pen to mark the slide(s) "O.K." so we always know who signed-off on the control slide. All positive controls are filed by date (the date is printed on the label). I believe that we can "back-track" (using the staining logs) to identify which machine the control was run on (if needed). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Amy Self 4/18/2012 9:55 AM >>> Good Morning and Happy Wednesday to everyone, For those of you that are running batch controls with your IHC stains I have a couple of questions for you. What is your process for review and documentation of the batch control slide? How do you label/identify this slide for easy retrieval? (Do you identify this slide by date run or stain run) Thanks in advance for everyone's help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Wed Apr 18 15:10:51 2012 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Apr 18 15:10:53 2012 Subject: [Histonet] Batch Controls In-Reply-To: <4F8EE09C.7400.0077.1@harthosp.org> References: <4F8EE09C.7400.0077.1@harthosp.org> Message-ID: <002601cd1d9f$5ac3f650$104be2f0$@com> What do you do if the IHC slides need to be sent to another facility. Currently we place a control on every slide we run. I know this is using a lot of tissue but I sometimes have 3 different cases going to 3 different facilities for patient treatment. Also we stain all the IHC stains for 5 different hospitals. I can't see any way of getting around putting the positive controls on the slides. With having 14 different Pathologists this seems to work out best. How is everyone handling this? Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, April 18, 2012 3:41 PM To: Amy Self; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Batch Controls As the laboratory Director, I can sign-off on the positive control slides or my staff can do it since I have delegated this responsibility to them. We all use a different color pen to mark the slide(s) "O.K." so we always know who signed-off on the control slide. All positive controls are filed by date (the date is printed on the label). I believe that we can "back-track" (using the staining logs) to identify which machine the control was run on (if needed). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Amy Self 4/18/2012 9:55 AM >>> Good Morning and Happy Wednesday to everyone, For those of you that are running batch controls with your IHC stains I have a couple of questions for you. What is your process for review and documentation of the batch control slide? How do you label/identify this slide for easy retrieval? (Do you identify this slide by date run or stain run) Thanks in advance for everyone's help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed Apr 18 18:04:42 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Apr 18 18:04:52 2012 Subject: [Histonet] Unregistered HT testing Message-ID: Hi Nicole, I am going to have to disagree with you on this one. Doing both regularly, I find IHC to be almost routine in comparison to some of the more complicated special stains. Specials depend on a good understanding of the chemistry involved and being able to make minor adjustments to get the perfect reaction. Consider when the last time you had to differentiate an IHC reaction... never. While it is commonplace in VVG, LFB and many other stains. I'm certainly not saying IHC is not complicated but one should never become complacent with those lowly specials. Regarding automation, sure anyone can load a slide onto a stainer and press go. That is not the point though. Automation should only mimic what you would do with your hands. Once we loose sight of that and let the computers outsmart us, we might as well be replaced by them. The instrument puts the periodic acid on first then the Schiff's reagent not because the computer told it to, but because I told the computer to tell the instrument to. Just like when driving a car you still need to pay attention to the road. When you loose sight of who is in control you will hit a deer and have a really bad day. Have a great day, (and don't kill any deer) Amos On Wed, Apr 18, 2012 at 10:26 AM, wrote: > Message: 8 > Date: Tue, 17 Apr 2012 14:37:09 -0500 > From: "Konop, Nicole" > Subject: [Histonet] Unregistered HT testing > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > < > A733875C1AA92D4EBD9A35A9506DFE3A5DC3415D3F@C1EXCPWV02.chwi.chswi.org> > Content-Type: text/plain; charset="us-ascii" > > Hello everyone! > > I'm just curious to know if anyone is allowing unregistered HT's to do > special stains in their CAP accredited lab? I have been involved in > discussions regarding high complexity testing. From the feedback I have > received, special stains and IHC stains are considered high complexity > testing. I beg to disagree. I can understand IHC/ISH as high complexity > but I don't think routine special stains fall under that category. I'd > appreciate any feedback or literature you can reference for me to review. > Thank you! > > Nicole Anne Konop BS, HTL(ASCP) > From madeleinehuey <@t> gmail.com Thu Apr 19 00:18:01 2012 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Thu Apr 19 00:18:11 2012 Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 23 In-Reply-To: <4f8ecf09.04013c0a.4e55.07f2SMTPIN_ADDED@mx.google.com> References: <4f8ecf09.04013c0a.4e55.07f2SMTPIN_ADDED@mx.google.com> Message-ID: Clare, I have NO background when with polyclonal Rabbit anti-PAX 8 from Cell Marque (RTU) & monoclonal Mouse anti-PAX 8 from Biocare (RTU) on Leica Bond nor Dako Plus (never Ventana - need prep kit). Rabbit-PAX 8 is more sensitive, but the mouse-PAX 8 is more specific, but both work well. Maybe we can help you if you give us a more detail protocol. Best, Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madeleinehuey@elcaminohospital.org > Date: Wed, 18 Apr 2012 10:04:35 -0400 > From: Clare Thornton > Subject: [Histonet] PAX-8 antibody > To: "'histonet@lists.utsouthwestern.edu'" > ? ? ? ? > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset="us-ascii" > > Looking for help with a protocol for this antibody using Ventana platform, either Ultra or Benchmark, Ultraview detection and Biocare's predilute antibody. ?Would like protocol for any other vendor's antibody as well. ?We are having issues with light staining and too much background. > > thank you! > > Clare J. Thornton, HTL(ASCP), QIHC > Assistant Histology Supervisor > Dahl-Chase Diagnostic Services > 417 State Street, Suite 540 > Bangor, ME 04401 > cthornton@dahlchase.com > > > > ------------------------------ From louise.renton <@t> gmail.com Thu Apr 19 02:32:27 2012 From: louise.renton <@t> gmail.com (Louise Renton) Date: Thu Apr 19 02:32:44 2012 Subject: [Histonet] 101 steps to better histology Message-ID: Hi all, Just thought I'd share this with y'all. I have just received a booklet from Leica Microssytems "101 steps to better histology", which i think is an excellent overview and ready reference for practical histology. It has great photos of common problems such as sections contaminated with squames, incomplete blueing, bubbles in sections etc. Disclaimer: This is my personal opinion. I have no interests in Leica other than purchasing their equipment now and then -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From dencrowl <@t> MIT.EDU Thu Apr 19 06:45:46 2012 From: dencrowl <@t> MIT.EDU (Denise G Crowley) Date: Thu Apr 19 06:46:10 2012 Subject: [Histonet] human samples in research lab Message-ID: <2241DEA1-97AF-40DE-9337-4C607CF3FB85@mit.edu> Hi all, I would like to get some advice from the experts. We are a research lab currently working primarily with mouse and zebrafish tissues. One of our researchers has access to tissues from local hospitals which have been processed and embedded. Having been in research and away from the clinical field for over 20 years, I am unaware of the PPE and required safety training required for working with primary human tissues. We currently observe reasonable safety measures such as lab coats and glasses when handling chemicals, and of course no eating, drinking, or open-toed shoes in the lab. But should we be wearing lab coats, glasses and gloves while sectioning human samples? And what about the legal aspects such as material transfer agreements and HIPAA rules? I have been avoiding this issue for years by encouraging researchers to have the slides cut at the hospitals supplying the tissues, but I may not be able to do this much longer. Any advice? Thanks, Denise Crowley Histology Facility Manager Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu From Karen.Heckford <@t> DignityHealth.org Thu Apr 19 07:03:00 2012 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Thu Apr 19 07:03:10 2012 Subject: [Histonet] Pinning Specimen Message-ID: <3328693C43A557458850CC37CE16CD1877954A10@chw-msg-829.chw.edu> Does anyone know where to get specimen boards that you can pin specimens to and then submerse in formalin? I ordered them a long time ago and cannot remember where I got them. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From Neil.Macintyre <@t> ed.ac.uk Thu Apr 19 07:10:57 2012 From: Neil.Macintyre <@t> ed.ac.uk (Neil Macintyre) Date: Thu Apr 19 07:11:11 2012 Subject: [Histonet] Citrate Buffer pH2.0 Message-ID: <201204191210.q3JCAvNF015606@lmtp1.ucs.ed.ac.uk> Hi Eric Thanks for your input regarding the Citrate buffer pH2.0. Yep this pH came as a surprise to me too. The data sheet from the company specifies 0.01M citrate at pH2.0 and a publication which used the same antibody also indicates a "modified citrate buffer" but doesn't give the exact p. I will be checking the information with the company as suggested but just wanted to see if anyone else out in Histoland had come across this previously. So far the unexpected indication is No. I'll let you know how I get on if I do have to use pH 2.0 and if it is successful. Best regards Neil MacIntyre CSci FIBMS Laboratory Manager Veterinary Pathology Unit The Royal(Dick)School of Veterinary Studies The University Of Edinburgh Easter Bush Campus Midlothian EH25 9RG 0131 650 6403/8802 The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336. From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Apr 19 07:21:05 2012 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Apr 19 07:22:52 2012 Subject: [Histonet] RE: Pinning Specimen In-Reply-To: <3328693C43A557458850CC37CE16CD1877954A10@chw-msg-829.chw.edu> References: <3328693C43A557458850CC37CE16CD1877954A10@chw-msg-829.chw.edu> Message-ID: We used to make our own. We would get a large shallow cardboard box, fill it with paraffin. Let it cool. Then peel the cardboard away.... You can submerse that in formalin. And you can custom cut them to whatever size you need. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF [Karen.Heckford@DignityHealth.org] Sent: Thursday, April 19, 2012 8:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pinning Specimen Does anyone know where to get specimen boards that you can pin specimens to and then submerse in formalin? I ordered them a long time ago and cannot remember where I got them. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Thu Apr 19 08:16:29 2012 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Apr 19 08:16:38 2012 Subject: [Histonet] Pinning Specimen In-Reply-To: <3328693C43A557458850CC37CE16CD1877954A10@chw-msg-829.chw.edu> References: <3328693C43A557458850CC37CE16CD1877954A10@chw-msg-829.chw.edu> Message-ID: Hi Karen - Are yoru eferring to the boards that have the detachable mats? I know they still make them and the last one I heard of was through MOPEC. I'm probably aging myself here, but we also used to make paraffin blocks for large specimens and pin them flat for overnight fixation - it wasn't pretty but it worked. Hope this helps some. Vikki On Thu, Apr 19, 2012 at 8:03 AM, Heckford, Karen - SMMC-SF < Karen.Heckford@dignityhealth.org> wrote: > Does anyone know where to get specimen boards that you can pin specimens > to and then submerse in formalin? I ordered them a long time ago and > cannot remember where I got them. > > Thanks, > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford@dignityhealth.org > > Caution: This email message, including all content and attachments, > is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you." > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From wbenton <@t> cua.md Thu Apr 19 08:59:03 2012 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Apr 19 09:02:53 2012 Subject: [Histonet] Pinning Specimen In-Reply-To: References: <3328693C43A557458850CC37CE16CD1877954A10@chw-msg-829.chw.edu>, Message-ID: <0B8979A204680A42B93A52B486088CD92C2416C3C0@CUAEXH1.GCU-MD.local> At previous institutions I purchased rolls or sheets of cork and that allowed us to pin and float samples like neck dissections, segments of colon etc.... I know Fisher sells the sheets and the roll of cork we purchased from an internet retailer. Just do a Google or Amazon search. Making the paraffin blocks is easy as well. You can take any plastic container and pour off fresh paraffin or use paraffin from a tissue processor when you are disposing of it to make molds. Hope this helps. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker [bakevictoria@gmail.com] Sent: Thursday, April 19, 2012 9:16 AM To: Heckford, Karen - SMMC-SF Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Pinning Specimen Hi Karen - Are yoru eferring to the boards that have the detachable mats? I know they still make them and the last one I heard of was through MOPEC. I'm probably aging myself here, but we also used to make paraffin blocks for large specimens and pin them flat for overnight fixation - it wasn't pretty but it worked. Hope this helps some. Vikki On Thu, Apr 19, 2012 at 8:03 AM, Heckford, Karen - SMMC-SF < Karen.Heckford@dignityhealth.org> wrote: > Does anyone know where to get specimen boards that you can pin specimens > to and then submerse in formalin? I ordered them a long time ago and > cannot remember where I got them. > > Thanks, > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford@dignityhealth.org > > Caution: This email message, including all content and attachments, > is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you." > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From ihcman2010 <@t> hotmail.com Thu Apr 19 09:19:41 2012 From: ihcman2010 <@t> hotmail.com (Glen Dawson) Date: Thu Apr 19 09:19:50 2012 Subject: [Histonet] Batch Controls In-Reply-To: <1334768356.79827.YahooMailClassic@web162105.mail.bf1.yahoo.com> References: , <1334768356.79827.YahooMailClassic@web162105.mail.bf1.yahoo.com> Message-ID: All, I place a positive control on each slide, next to the patient tissue for all of the reasons already mentioned, but we are missing the obvious one. Many of us use some kind of automated immunostainer where there is no "gaurantee" that, because the CD3 in position #4 (batch control) worked, the CD3's loaded in positions 6, 9, 13, and 21 also worked. Perhaps a reagent ran out or there was air in a line for part of the process for any one of these other CD3's and, because there is no control on the same slide, there may be a false negative result reported due to the use of a batch control. For this reason alone, one should think hard about using batch controls. Just My Opinion, Glen Dawson BS, HT(ASCP) & QIHC Histology Technical Specialist Janesville, WI From sdysart <@t> mirnarx.com Thu Apr 19 09:36:13 2012 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Thu Apr 19 09:36:38 2012 Subject: [Histonet] Batch Controls In-Reply-To: References: , <1334768356.79827.YahooMailClassic@web162105.mail.bf1.yahoo.com> Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5001D62F8F@BL2PRD0710MB363.namprd07.prod.outlook.com> I still use batch controls, but I am one of the few left that is not automated. I do everything by hand. I think you are right though, placing a control tissue on each slide is the only way to be sure that everything was dispensed correctly...especially if you are using a Ventana... Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Glen Dawson Sent: Thursday, April 19, 2012 9:20 AM To: histonet Subject: RE: [Histonet] Batch Controls All, I place a positive control on each slide, next to the patient tissue for all of the reasons already mentioned, but we are missing the obvious one. Many of us use some kind of automated immunostainer where there is no "gaurantee" that, because the CD3 in position #4 (batch control) worked, the CD3's loaded in positions 6, 9, 13, and 21 also worked. Perhaps a reagent ran out or there was air in a line for part of the process for any one of these other CD3's and, because there is no control on the same slide, there may be a false negative result reported due to the use of a batch control. For this reason alone, one should think hard about using batch controls. Just My Opinion, Glen Dawson BS, HT(ASCP) & QIHC Histology Technical Specialist Janesville, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSiena <@t> statlab.com Thu Apr 19 09:46:45 2012 From: DSiena <@t> statlab.com (Debra Siena) Date: Thu Apr 19 09:46:32 2012 Subject: [Histonet] Batch Controls In-Reply-To: References: , <1334768356.79827.YahooMailClassic@web162105.mail.bf1.yahoo.com> Message-ID: After spending 7 1/2 years as a technical specialist with Ventana, I would also like to say that putting the control on the bottom of the slide and the patient at the top is just added insurance that the patient will receive the bulk of the reagents vs. the control which will help ensure against false negatives should there be a staining issue. Just my 2 cents worth. thanks ? Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t:?800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Glen Dawson Sent: Thursday, April 19, 2012 9:20 AM To: histonet Subject: RE: [Histonet] Batch Controls All, I place a positive control on each slide, next to the patient tissue for all of the reasons already mentioned, but we are missing the obvious one. Many of us use some kind of automated immunostainer where there is no "gaurantee" that, because the CD3 in position #4 (batch control) worked, the CD3's loaded in positions 6, 9, 13, and 21 also worked. Perhaps a reagent ran out or there was air in a line for part of the process for any one of these other CD3's and, because there is no control on the same slide, there may be a false negative result reported due to the use of a batch control. For this reason alone, one should think hard about using batch controls. Just My Opinion, Glen Dawson BS, HT(ASCP) & QIHC Histology Technical Specialist Janesville, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sameena.k.masood <@t> Vanderbilt.Edu Thu Apr 19 10:05:23 2012 From: sameena.k.masood <@t> Vanderbilt.Edu (Masood, Sameena Kathryn) Date: Thu Apr 19 10:05:04 2012 Subject: [Histonet] Shandon Hypercenter XP processor In-Reply-To: References: <5D59AF96B902456A8B5948EB08BACE79@PATHOLOGY> Message-ID: <1D0DCB1F02F24E4AB356F0F2EAEE1DC7061377F85E@its-hcwnem05.ds.Vanderbilt.edu> Do any of you know of companies or individuals that can repair an old Shandon Hypercenter XP processor? They don't even make parts for it anymore. From JCBRITTON <@t> Cheshire-Med.COM Thu Apr 19 10:22:21 2012 From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C) Date: Thu Apr 19 10:21:58 2012 Subject: [Histonet] Tissue Problem In-Reply-To: <466B666475DE6547BBB0641E540A4BB5115B4B75EC@EXVS1.meriter.com> References: <466B666475DE6547BBB0641E540A4BB5115B4B75EC@EXVS1.meriter.com> Message-ID: Sounds like the specimens are not fixed well enough! Josie Britton HT(ASCP) Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peterson, Dan Sent: Wednesday, April 18, 2012 1:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue Problem OK netters, I've got a problem that's just cropped up over the last week or so. It's happening to our larger specimens, and only the ones containing tumor. When we cut a slide, the tissue looks processed well, but as the slide dries, the tissue area turns really white. Then when we try and stain it (especially Immunos) just that portion of the section falls off. Any clues to defeat this gremlin would be greatly appreciated. Dan Daniel R Peterson HT(ASCP) Histopathology Technical Specialist Meriter Laboratories (608) 417-6557 fax (608) 417-6343 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmcgough <@t> clinlab.com Thu Apr 19 10:24:06 2012 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Thu Apr 19 10:24:29 2012 Subject: [Histonet] "Leased" tech to GI practice In-Reply-To: Message-ID: I am wondering if any anatomic pathology labs out there have "leased" a FTE, histology tech, to a GI practice to run a histology lab in the Endoscopy clinic? How did you logistically accomplish this? You can contact me offline for more details. Thank you in advance for your responses. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com From cmiller <@t> physlab.com Thu Apr 19 10:32:51 2012 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Apr 19 10:33:02 2012 Subject: [Histonet] grossing tech Message-ID: I have a grossing position open, I have a non certified. Non degreed awesome tech with 27 years. Anyway I can have her Gran-fathered in by CAP regulations? I'm pretty sure I know the answer I am hoping to get around this, Cheri Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From TGoins <@t> mt.gov Thu Apr 19 11:33:46 2012 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Apr 19 11:33:58 2012 Subject: [Histonet] RE: Unregistered HT testing In-Reply-To: References: Message-ID: I disagree with your assessment of complex staining. IHC staining is like "cookie-cutter" staining - one does the same steps every single time with a different (but very similar) set of reagents. The quality of special stains on the other hand are determined by a unique chemistry - one can get it wrong in so many ways. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, April 17, 2012 1:37 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unregistered HT testing Hello everyone! I'm just curious to know if anyone is allowing unregistered HT's to do special stains in their CAP accredited lab? I have been involved in discussions regarding high complexity testing. From the feedback I have received, special stains and IHC stains are considered high complexity testing. I beg to disagree. I can understand IHC/ISH as high complexity but I don't think routine special stains fall under that category. I'd appreciate any feedback or literature you can reference for me to review. Thank you! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Apr 19 12:20:34 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Apr 19 12:20:43 2012 Subject: [Histonet] RE: Unregistered HT testing In-Reply-To: References: , Message-ID: Tresa I do see your point. I guess to me setting up IHC and validation takes some insight and knowledge, but once it is running on a platform, it is pretty much the same as far as your hands on and gets pretty routine. Those that get to still do manual IHC get a little more challenge. Since I just do automated and I am not allowed to participate or alter protocols even when I think I should, it does get pretty repetitive, and of course you are right- its basically the same groups of reagents and reaction you carry out, albeit with different more specific targets. I do personally think that doing specials does require a lot more chemistry/ reaction knowledge, and tissue knowledge, since each stain has a unique chemistry, reaction, results etc. I especially believe that for manual special stains. Remember when you had to differeniate stains under the microscope? Nowadays I see many techs that never even look under the 'scope! Anyhow, that is why I liked doing them that way best, and I really kind of miss it. I wish that people still had to know why/what/how of doing those stains even if they use an instrument to carry them out, but that is "opinion" not necessarily shared by everyone. I think using automation for either specials or IHC can make you lazy in a way, though it helps standardization and TAT. But unfortunately, the powers that be don't seem to see it that way. As many have pointed out, CLIA does not recognize anything in histology as high complexity except for IHC/ISH. Of course it was put out in 1988 and probably no histology/legislators were involved in the categorization of testing. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: TGoins@mt.gov > To: NKonop@chw.org; histonet@lists.utsouthwestern.edu > Date: Thu, 19 Apr 2012 16:33:46 +0000 > CC: > Subject: [Histonet] RE: Unregistered HT testing > > I disagree with your assessment of complex staining. IHC staining is like "cookie-cutter" staining - one does the same steps every single time with a different (but very similar) set of reagents. The quality of special stains on the other hand are determined by a unique chemistry - one can get it wrong in so many ways. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole > Sent: Tuesday, April 17, 2012 1:37 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Unregistered HT testing > > Hello everyone! > > I'm just curious to know if anyone is allowing unregistered HT's to do special stains in their CAP accredited lab? I have been involved in discussions regarding high complexity testing. From the feedback I have received, special stains and IHC stains are considered high complexity testing. I beg to disagree. I can understand IHC/ISH as high complexity but I don't think routine special stains fall under that category. I'd appreciate any feedback or literature you can reference for me to review. Thank you! > > Nicole Anne Konop BS, HTL(ASCP) > Histology Team Lead > Children's Hospital of Wisconsin > (414)266-6580 Direct Line > (414)907-0366 Pager > (414)266-2524 Histology Department > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Thu Apr 19 12:31:07 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Apr 19 12:31:24 2012 Subject: [Histonet] RE: Unregistered HT testing In-Reply-To: References: Message-ID: <4B5F2E7B-6DE7-47C2-9D6F-3194B047EDEE@yahoo.com> As usual we all have our own opinion. High complexity acceding to CLIA is a defined measurement. In other words things like is the task heat, ph , time dependent, accurate measuring ? Those examples make a task high complexity. The above us exactly why grossing is considered high complexity by CLIA. Don't forget that CAP standards are equivalent or higher than CLIA And the important difference to remember is not that someone knows how to push a button. But they know what to do when the button won't work I hope all of us as professionals always seek to raise the standards and education of our task and not to always seek less of what is expected of us. Have a good one! Kim D Sent from my iPhone On Apr 19, 2012, at 1:20 PM, joelle weaver wrote: > > Tresa I do see your point. I guess to me setting up IHC and validation takes some insight and knowledge, but once it is running on a platform, it is pretty much the same as far as your hands on and gets pretty routine. Those that get to still do manual IHC get a little more challenge. Since I just do automated and I am not allowed to participate or alter protocols even when I think I should, it does get pretty repetitive, and of course you are right- its basically the same groups of reagents and reaction you carry out, albeit with different more specific targets. I do personally think that doing specials does require a lot more chemistry/ reaction knowledge, and tissue knowledge, since each stain has a unique chemistry, reaction, results etc. I especially believe that for manual special stains. Remember when you had to differeniate stains under the microscope? Nowadays I see many techs that never even look under the 'scope! Anyhow, that is why I liked doing them that way best, and I really kind of miss it. I wish that people still had to know why/what/how of doing those stains even if they use an instrument to carry them out, but that is "opinion" not necessarily shared by everyone. I think using automation for either specials or IHC can make you lazy in a way, though it helps standardization and TAT. But unfortunately, the powers that be don't seem to see it that way. As many have pointed out, CLIA does not recognize anything in histology as high complexity except for IHC/ISH. Of course it was put out in 1988 and probably no histology/legislators were involved in the categorization of testing. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC >> From: TGoins@mt.gov >> To: NKonop@chw.org; histonet@lists.utsouthwestern.edu >> Date: Thu, 19 Apr 2012 16:33:46 +0000 >> CC: >> Subject: [Histonet] RE: Unregistered HT testing >> >> I disagree with your assessment of complex staining. IHC staining is like "cookie-cutter" staining - one does the same steps every single time with a different (but very similar) set of reagents. The quality of special stains on the other hand are determined by a unique chemistry - one can get it wrong in so many ways. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole >> Sent: Tuesday, April 17, 2012 1:37 PM >> To: 'histonet@lists.utsouthwestern.edu' >> Subject: [Histonet] Unregistered HT testing >> >> Hello everyone! >> >> I'm just curious to know if anyone is allowing unregistered HT's to do special stains in their CAP accredited lab? I have been involved in discussions regarding high complexity testing. From the feedback I have received, special stains and IHC stains are considered high complexity testing. I beg to disagree. I can understand IHC/ISH as high complexity but I don't think routine special stains fall under that category. I'd appreciate any feedback or literature you can reference for me to review. Thank you! >> >> Nicole Anne Konop BS, HTL(ASCP) >> Histology Team Lead >> Children's Hospital of Wisconsin >> (414)266-6580 Direct Line >> (414)907-0366 Pager >> (414)266-2524 Histology Department >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Apr 19 12:32:33 2012 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Apr 19 12:32:42 2012 Subject: [Histonet] RE: Unregistered HT testing In-Reply-To: <4B5F2E7B-6DE7-47C2-9D6F-3194B047EDEE@yahoo.com> References: , <4B5F2E7B-6DE7-47C2-9D6F-3194B047EDEE@yahoo.com> Message-ID: Yes, I wish for that too. Joelle Weaver MAOM, HTL (ASCP) QIHC > CC: tgoins@mt.gov; nkonop@chw.org; histonet@lists.utsouthwestern.edu > From: one_angel_secret@yahoo.com > Subject: Re: [Histonet] RE: Unregistered HT testing > Date: Thu, 19 Apr 2012 13:31:07 -0400 > To: joelleweaver@hotmail.com > > As usual we all have our own opinion. > > High complexity acceding to CLIA is a defined measurement. In other words things like is the task heat, ph , time dependent, accurate measuring ? Those examples make a task high complexity. > > The above us exactly why grossing is considered high complexity by CLIA. > > Don't forget that CAP standards are equivalent or higher than CLIA > > And the important difference to remember is not that someone knows how to push a button. But they know what to do when the button won't work > > I hope all of us as professionals always seek to raise the standards and education of our task and not to always seek less of what is expected of us. > > Have a good one! > > Kim D > > Sent from my iPhone > > On Apr 19, 2012, at 1:20 PM, joelle weaver wrote: > > > > > Tresa I do see your point. I guess to me setting up IHC and validation takes some insight and knowledge, but once it is running on a platform, it is pretty much the same as far as your hands on and gets pretty routine. Those that get to still do manual IHC get a little more challenge. Since I just do automated and I am not allowed to participate or alter protocols even when I think I should, it does get pretty repetitive, and of course you are right- its basically the same groups of reagents and reaction you carry out, albeit with different more specific targets. I do personally think that doing specials does require a lot more chemistry/ reaction knowledge, and tissue knowledge, since each stain has a unique chemistry, reaction, results etc. I especially believe that for manual special stains. Remember when you had to differeniate stains under the microscope? Nowadays I see many techs that never even look under the 'scope! Anyhow, that is why I liked doing them that way best, and I really kind of miss it. I wish that people still had to know why/what/how of doing those stains even if they use an instrument to carry them out, but that is "opinion" not necessarily shared by everyone. I think using automation for either specials or IHC can make you lazy in a way, though it helps standardization and TAT. But unfortunately, the powers that be don't seem to see it that way. As many have pointed out, CLIA does not recognize anything in histology as high complexity except for IHC/ISH. Of course it was put out in 1988 and probably no histology/legislators were involved in the categorization of testing. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > >> From: TGoins@mt.gov > >> To: NKonop@chw.org; histonet@lists.utsouthwestern.edu > >> Date: Thu, 19 Apr 2012 16:33:46 +0000 > >> CC: > >> Subject: [Histonet] RE: Unregistered HT testing > >> > >> I disagree with your assessment of complex staining. IHC staining is like "cookie-cutter" staining - one does the same steps every single time with a different (but very similar) set of reagents. The quality of special stains on the other hand are determined by a unique chemistry - one can get it wrong in so many ways. > >> > >> -----Original Message----- > >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole > >> Sent: Tuesday, April 17, 2012 1:37 PM > >> To: 'histonet@lists.utsouthwestern.edu' > >> Subject: [Histonet] Unregistered HT testing > >> > >> Hello everyone! > >> > >> I'm just curious to know if anyone is allowing unregistered HT's to do special stains in their CAP accredited lab? I have been involved in discussions regarding high complexity testing. From the feedback I have received, special stains and IHC stains are considered high complexity testing. I beg to disagree. I can understand IHC/ISH as high complexity but I don't think routine special stains fall under that category. I'd appreciate any feedback or literature you can reference for me to review. Thank you! > >> > >> Nicole Anne Konop BS, HTL(ASCP) > >> Histology Team Lead > >> Children's Hospital of Wisconsin > >> (414)266-6580 Direct Line > >> (414)907-0366 Pager > >> (414)266-2524 Histology Department > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From careerstudio <@t> bellsouth.net Thu Apr 19 12:38:55 2012 From: careerstudio <@t> bellsouth.net (Career Studio) Date: Thu Apr 19 12:38:57 2012 Subject: [Histonet] Histology Manager position with our clinical reference lab client in southern California Message-ID: <00ea01cd1e53$4bbf75d0$e33e6170$@net> Our client is a leading clinical laboratory currently seeking a Histology/IHC Manager, Monday thru Friday, to oversee operation & administration of several departments. Based in southern California, this individual will work closely with pathologists & other medical professionals to effectively meet operation goals, work with tight timelines and ensure that histology staff adheres to these needs. while conducting high quality work. Specimens: slides, blocks, previously cut....some Grossing.....Bone Marrow and Aspirate. Responsibilities will include: . Management of all day-to-day operations technical activities of IHC/Histology/Imaging Department, including staffing, planning, coordination and evaluation . Troubleshooting quality and production issues including stains, processes, systems, and equipment. . Developing and implementing improvements in systems and processes for enhanced efficiency and quality, against corporate and departmental and goals. Candidate must have BA/BS in Biology, Chemistry or related science, with 4+ years of experience in a clinical lab including at least 3 years of supervisory experience. HT (ASCP); and QIHC qualification helpful. Must meet CLIA requirements. In addition, superior organizational and operational skills and the ability to manage multiple priorities and timelines effectively are needed. Qualities in this individual will be: self-motivated, detail oriented, excellent communication skills, and thriving with high degree of responsibility. Offered for this position is compensation commensurate with experience to attract top talent, with bonus package, & relocation assistance for California licensed individual. . Please contact David King at biolabcareers@aol.com for more information about this opportunity. David King Career Studio national search biolabcareers@aol.com 561-738-6363 Visit us on linkedin: http://www.linkedin.com/in/biotechnologyhires From rford <@t> hcmhcares.org Thu Apr 19 13:10:07 2012 From: rford <@t> hcmhcares.org (Rhonda Ford) Date: Thu Apr 19 13:10:12 2012 Subject: [Histonet] Sakura VIP 3000 Tissue Processor In-Reply-To: <98CC14B915EBA84B9A326D45CC3C1DEC9A479905@cbiolabs05.CBiolabs.local> References: <98CC14B915EBA84B9A326D45CC3C1DEC9A479905@cbiolabs05.CBiolabs.local> Message-ID: Marston Technical from Cincinnati, Ohio has supplies. Their phone is 513-563-8100. On Wed, Apr 18, 2012 at 3:35 PM, Lyn Stadler wrote: > Anyone out there using a VIP 3000? I need some replacement gaskets that > Sakuara no longer manufactures. Anyone know of an alternate vendor or > supplier that carries them? Or, better yet, anyone have some that they are > interested in giving away ;). > > Thanks in advance! > > > > > This communication may contain privileged information. It is intended > solely for the use of the addressee. If you are not the intended > recipient, you are strictly prohibited from disclosing, copying, > distributing or using any of this information. If you received this > communication in error, please contact the sender immediately and destroy > the material in its entirety, whether electronic or hard copy. This > communication may contain nonpublic information about individuals and > businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You > may not directly or indirectly reuse or redisclose such information for any > purpose other than to provide the services for which you are receiving the > information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Rhonda Ford, Histology Lab Henry County Hospital (765) 521-1148 From Allison_Scott <@t> hchd.tmc.edu Thu Apr 19 14:22:56 2012 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Thu Apr 19 14:23:00 2012 Subject: [Histonet] GMS on Toenail Message-ID: Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From kkienitz <@t> orclinic.com Thu Apr 19 14:33:19 2012 From: kkienitz <@t> orclinic.com (Kienitz, Kari) Date: Thu Apr 19 14:36:07 2012 Subject: [Histonet] RE: GMS on Toenail In-Reply-To: References: Message-ID: <41400FFE517878449D89114DD25260900848688BF2@tocmail1.tocad.orclinic.com> After cutting, try putting the slide into a coplin jar of formalin. Introduce to heat for about 30 minutes or so, remove and let air dry before staining. Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz@orclinic.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D [Allison_Scott@hchd.tmc.edu] Sent: Thursday, April 19, 2012 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Thu Apr 19 14:44:34 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Apr 19 14:44:37 2012 Subject: [Histonet] Re: Pinning Specimen Message-ID: Karen Heckford HT ASCP CE at St. Mary's Medical Center in San Francisco asks: >>Does anyone know where to get specimen boards that you can pin specimens to and then submerse in formalin? I ordered them a long time ago and cannot remember where I got them.<< I've solved this problem several ways. Probably the best, which has already been mentioned, is to have blocks cast from waste paraffin, in several sizes, and pin the specimen to them with those T-shaped steel "map pins" and put it face down in the fixative. You can also use cardboard or styrofoam. You may need to put a weight on top of the pinning board. Most specimens need fixing overnight. You can ink before or after fixation. Most of the pathology services I've worked in do not have such pinning arrangements and do not welcome them. Does anyone know what the Hospital Administrator and Lab Manager's Handy-Dandy Manual for Tying the Pathologist in Knots has to say about them? (The top-secret book they all have in their desk drawers.) Bob Richmond Samurai Pathologist Knoxville TN From NMargaryan <@t> childrensmemorial.org Thu Apr 19 15:07:43 2012 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Apr 19 15:09:02 2012 Subject: [Histonet] proliferation and apoptosis Message-ID: Hi histonetters, I am looking for the good markers to detect (separately) proliferation and apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic bodies as well as proliferated cells; and the tunnel assay shows both apoptotic body and proliferation. Any suggestions for the FFPE tissue and the HRP protocol are appreciated. Thanks in advance, Naira From tora.bardal <@t> bio.ntnu.no Thu Apr 19 15:43:04 2012 From: tora.bardal <@t> bio.ntnu.no (Tora Bardal) Date: Thu Apr 19 15:43:18 2012 Subject: [Histonet] hepes instead of cacodylate Message-ID: <4F9078D8.1050904@bio.ntnu.no> Hi I'm trying to switch from cacodylate buffer to Hepes in my EM-fixative. PBS buffer is not an option. My fixative contains sucrose, CaCl2, 2,5 % formaldehyde and 2,5% GA in addition to cacodylate (later Hepes). Formaldehyde is "homemade" stock 25 % (no methanol) After storage for some time in Hepes fixative I see a turbidity/precipitate. Stored fixative with precipitate and newly mixed fixative gave the same pH. Another group who made this Hepes fixative after my recipe told me they got precipitate once they mixed in the GA. What have I missed here when it comes to chemicals and reactions? Any good protocols out there? Tora Bardal NTNU Sealab From b427297 <@t> aol.com Thu Apr 19 16:23:09 2012 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Thu Apr 19 16:23:27 2012 Subject: [Histonet] proliferation and apoptosis In-Reply-To: References: Message-ID: <8CEEC89C0D22730-1EAC-511CA@webmail-d017.sysops.aol.com> Ki67 does not stain apoptotic cells. Why do you have that impression? Caspase-3 is a great marker for apoptotic cells. TUNEL will show apoptosis as well as necrosis, but not proliferation. I've used Ki67 for proliferation and Caspase 3 for apoptosis routinely in cancer research for years. Jackie -----Original Message----- From: Margaryan, Naira To: histonet-request Cc: histonet Sent: Thu, Apr 19, 2012 3:09 pm Subject: [Histonet] proliferation and apoptosis Hi histonetters, I am looking for the good markers to detect (separately) proliferation and apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic bodies as well as proliferated cells; and the tunnel assay shows both apoptotic body and proliferation. Any suggestions for the FFPE tissue and the HRP protocol are appreciated. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cls71877 <@t> sbcglobal.net Thu Apr 19 16:24:49 2012 From: cls71877 <@t> sbcglobal.net (=?utf-8?B?Y2xzNzE4NzdAc2JjZ2xvYmFsLm5ldA==?=) Date: Thu Apr 19 16:24:57 2012 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gUkU6IEdNUyBvbiBUb2VuYWls?= Message-ID: <750487.52406.bm@smtp204.mail.bf1.yahoo.com> Are using any adhesive on the slide or a charged slide? We used to dip slides in a water/elmer glue solution and allow to dry. Then place a section on it and it seemed to work... Sent from my HTC on the Now Network from Sprint! ----- Reply message ----- From: "Kienitz, Kari" Date: Thu, Apr 19, 2012 12:33 pm Subject: [Histonet] RE: GMS on Toenail To: "Scott, Allison D" , "histonet@lists.utsouthwestern.edu" After cutting, try putting the slide into a coplin jar of formalin. Introduce to heat for about 30 minutes or so, remove and let air dry before staining. Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz@orclinic.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D [Allison_Scott@hchd.tmc.edu] Sent: Thursday, April 19, 2012 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Apr 19 16:37:26 2012 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Apr 19 16:37:32 2012 Subject: [Histonet] proliferation and apoptosis In-Reply-To: <8CEEC89C0D22730-1EAC-511CA@webmail-d017.sysops.aol.com> Message-ID: <14E2C6176416974295479C64A11CB9AE011390CC5C2E@SBS2K8.premierlab.local> I agree with Jackie, we use Ki-67 all of the time and I have never seen it stain apoptotic cells, could you possibly be dealing with some background staining due to the detection system used? I do not know what type of samples you are staining? We have 4 different Ki-67 antibodies we use depending upon the type of tissue we are staining. For human xenograft samples in a mouse background we use the rabbit polyclonal from Santa Cruz. We also use cleaved caspase 3 as a marker of apoptosis routinely. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Thursday, April 19, 2012 3:23 PM To: NMargaryan@childrensmemorial.org; histonet-request@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] proliferation and apoptosis Ki67 does not stain apoptotic cells. Why do you have that impression? Caspase-3 is a great marker for apoptotic cells. TUNEL will show apoptosis as well as necrosis, but not proliferation. I've used Ki67 for proliferation and Caspase 3 for apoptosis routinely in cancer research for years. Jackie -----Original Message----- From: Margaryan, Naira To: histonet-request Cc: histonet Sent: Thu, Apr 19, 2012 3:09 pm Subject: [Histonet] proliferation and apoptosis Hi histonetters, I am looking for the good markers to detect (separately) proliferation and apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic bodies as well as proliferated cells; and the tunnel assay shows both apoptotic body and proliferation. Any suggestions for the FFPE tissue and the HRP protocol are appreciated. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jfray80 <@t> hotmail.com Thu Apr 19 16:58:08 2012 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Thu Apr 19 16:58:12 2012 Subject: [Histonet] LIS Message-ID: Anyone know if there is or has used a good Anatomical Path LIS that is compatible or user friendly with Greenway. I really don't know a whole lot about this stuff , but could use some ideas. Thanks Histojoe From mehlikafaire <@t> hotmail.com Thu Apr 19 18:07:05 2012 From: mehlikafaire <@t> hotmail.com (Mehlika Faire) Date: Thu Apr 19 18:07:12 2012 Subject: [Histonet] proliferation and apoptosis In-Reply-To: <14E2C6176416974295479C64A11CB9AE011390CC5C2E@SBS2K8.premierlab.local> References: <8CEEC89C0D22730-1EAC-511CA@webmail-d017.sysops.aol.com>, <14E2C6176416974295479C64A11CB9AE011390CC5C2E@SBS2K8.premierlab.local> Message-ID: I've used pHH3 to mark proliferative cells and it works pretty well. Active-caspase-3 and cleaved-parp have worked well for staining apoptotic cells as well. -Mehlika > From: liz@premierlab.com > To: b427297@aol.com; NMargaryan@childrensmemorial.org; histonet-request@lists.utsouthwestern.edu > Date: Thu, 19 Apr 2012 15:37:26 -0600 > Subject: RE: [Histonet] proliferation and apoptosis > CC: histonet@lists.utsouthwestern.edu > > I agree with Jackie, we use Ki-67 all of the time and I have never seen it stain apoptotic cells, could you possibly be dealing with some background staining due to the detection system used? I do not know what type of samples you are staining? We have 4 different Ki-67 antibodies we use depending upon the type of tissue we are staining. For human xenograft samples in a mouse background we use the rabbit polyclonal from Santa Cruz. We also use cleaved caspase 3 as a marker of apoptosis routinely. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308-1592 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor > Sent: Thursday, April 19, 2012 3:23 PM > To: NMargaryan@childrensmemorial.org; histonet-request@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] proliferation and apoptosis > > > Ki67 does not stain apoptotic cells. Why do you have that impression? Caspase-3 is a great marker for apoptotic cells. TUNEL will show apoptosis as well as necrosis, but not proliferation. I've used Ki67 for proliferation and Caspase 3 for apoptosis routinely in cancer research for years. > > Jackie > > > -----Original Message----- > From: Margaryan, Naira > To: histonet-request > Cc: histonet > Sent: Thu, Apr 19, 2012 3:09 pm > Subject: [Histonet] proliferation and apoptosis > > > Hi histonetters, > > I am looking for the good markers to detect (separately) proliferation and > apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic > bodies as well as proliferated cells; and the tunnel assay shows both apoptotic > body and proliferation. > > Any suggestions for the FFPE tissue and the HRP protocol are appreciated. > > Thanks in advance, > Naira > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjann3 <@t> verizon.net Fri Apr 20 07:26:40 2012 From: rjann3 <@t> verizon.net (Robert Jann) Date: Fri Apr 20 07:26:58 2012 Subject: [Histonet] re: Pin 4 Message-ID: <9DEFD828F0C54AE389726D62944F408B@HOMEPC> Karen, Here's our protocol for FFPE sections on the Benchmark XT. Be advised that the times will be approximate for you and that we use Dako P504s @ 1:50. Also, you can call Ventana and request a rep. to come to your site and help you optimize the stain. 1. Create/manage protocols 2. select IHC DS uDAB - uRed v3 3. select deparaffinization 4. select cell conditioning - mild CC1 5. select antibody - 34BE12 + p63 (4536) for 8 min. 6. select ultrawash 7. select antibody denaturization - 95 deg. C for 4 min. 8. select DS antibody - Prep kit 1 (2851) (P504s) for 48 min. 9. select DS ultrawash 10. select counter stain - hematoxylin for 4 min. and blue for 4 min. You will also need to use the ultraview universal DAB detection kit and ultra view universal alkaline phosphatase red detection kit. Good luck in setting it up. Bob Jann HT(ASCP) From Mhorne <@t> upei.ca Fri Apr 20 07:43:03 2012 From: Mhorne <@t> upei.ca (Margaret Horne) Date: Fri Apr 20 07:43:23 2012 Subject: [Histonet] Re: Pinning Specimen In-Reply-To: References: Message-ID: <4F912FA7020000D1000127AD@oes-grpwise.novell.upei.ca> Just another idea : I have used dental wax. Comes in a package of about 20 sheets pf pink wax, each 7.5 cm x 14.5 cm x 0.2 cm. It's a little bit more pliable than the waste paraffin, but not as cheap :-) I cut it in a long rectangle so that it sticks out of the formalin with the tissue at the bottom end , rather like celery in a Bloody Caesar. Margaret >>> Bob Richmond 19/04/2012 4:44 PM >>> Karen Heckford HT ASCP CE at St. Mary's Medical Center in San Francisco asks: >>Does anyone know where to get specimen boards that you can pin specimens to and then submerse in formalin? I ordered them a long time ago and cannot remember where I got them.<< I've solved this problem several ways. Probably the best, which has already been mentioned, is to have blocks cast from waste paraffin, in several sizes, and pin the specimen to them with those T-shaped steel "map pins" and put it face down in the fixative. You can also use cardboard or styrofoam. You may need to put a weight on top of the pinning board. Most specimens need fixing overnight. You can ink before or after fixation. Most of the pathology services I've worked in do not have such pinning arrangements and do not welcome them. Does anyone know what the Hospital Administrator and Lab Manager's Handy-Dandy Manual for Tying the Pathologist in Knots has to say about them? (The top-secret book they all have in their desk drawers.) Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aajl7979 <@t> hotmail.com Fri Apr 20 08:10:08 2012 From: aajl7979 <@t> hotmail.com (Amanda L) Date: Fri Apr 20 08:10:17 2012 Subject: [Histonet] Part Time Job Opening, Springfield, MA Message-ID: Please Send Resume for: Part Time opportunity for a Histotechnican. Duties and responsibilities include: ? Under general supervision performs routine and non-routine activities involved in the preparation of slides, for microscopic evaluation by pathologist(s) according to policies and procedures ? Process paperwork associated with accessioning and reporting. ? Eligible to gross per CLIA requirements. ? Ensure proper tissue processing. ? Embed processed tissue in paraffin ? Prepare slides for routine staining. ? Perform microtomy of embedded tissue ? Perform coverslipping of stained slides either manually or automated. ? Perform filing of finished blocks and slides. ? Perform routine maintenance and cleaning of equipment and troubleshoot minor equipment failures. Document remedial actions such as repairs or repeated tests. ? Adhere to laboratory's quality control policies, and document all quality control activities. ? Ensure all corporate safety, quality control and quality assurance standards are met. ? Ensure compliance with all local, federal, CLIA and CAP regulations. ? Maintain a clean and well-organized work area. ? Clinical Chemistry & other duties, as assigned by supervisor. Minimum Requirements Include: ? AA or AS degree or equivalent training and experience , CLIA approved for grossing specimens ? HT (ASCP) or HTL (ASCP) eligible OR five years of full time experience in the last 10 years. ? Work Experience: 1-3 years experience as a Histotechnician in an anatomic pathology laboratory. ? License for Clinical Laboratory Technician or Technologist license. ***Note this is a part time position also with coverage for vacation Thanks! From etambutte <@t> centrescientifique.mc Fri Apr 20 12:03:08 2012 From: etambutte <@t> centrescientifique.mc (Eric Tambutte) Date: Fri Apr 20 12:03:16 2012 Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 27 Message-ID: <1083480164@s15272523.onlinehome-server.info> Bonjour, Je suis absent du laboratoire jusqu'au jeudi 03 mai 2012. Je vous répondrai le plus rapidement possible. Eric Tambutté Thank you for your mail. I will be out of office till May 03rd 2012. I will respond to your e-mail as soon as possible. Thank you for your understanding. Best regards Eric Tambutté From rsrichmond <@t> gmail.com Fri Apr 20 12:20:15 2012 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Fri Apr 20 12:20:21 2012 Subject: [Histonet] Re: Pinning Specimens Message-ID: About pinning specimens so they fix flat: Margaret Horne notes the use of dental wax. I've used it to make small "boats" for the rather exacting procedure of pinning muscle biopsy specimens for electron microscopy. Here a 1 to 2 mm bundle of longitudinal fibers has to be gently stretched to its resting length, pinned in the "boat", and promptly fixed in glutaraldehyde. One technologist noted that she had a young pathologist who was "wet behind the ears" and wanted to pin specimens, and was looking to the older pathologists to bring him in line to The Way We've Always Done It. I try to remain "wet behind the ears", though after nearly 50 years of occasionally pinning specimens I find myself a little dry. We continue to lose skills in the gross room. Today I'm working with a service that has almost no ability to handle calcified tissue - I need to go to the hardware store and buy a hacksaw to replace the worn-out Satterlee saw, which I think was gathered up off a Civil War battlefield. Bob Richmond Samurai Pathologist Knoxville TN From a.thotakura <@t> imperial.ac.uk Fri Apr 20 13:01:17 2012 From: a.thotakura <@t> imperial.ac.uk (Thotakura, Anil Kumar) Date: Fri Apr 20 13:01:46 2012 Subject: [Histonet] Immunofluorescence Message-ID: Dear All, Can some one please send the protocol for immunofluorescence, I want to stain for FITC CD45.1 on mice spleens. Thank you very much for your help. Many Thanks, Anil kumar. From JCBRITTON <@t> Cheshire-Med.COM Fri Apr 20 13:54:29 2012 From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C) Date: Fri Apr 20 13:53:39 2012 Subject: [Histonet] GMS on Toenail In-Reply-To: References: Message-ID: The way we got around that problem was to do a manual PAS stain instead! Josie Britton HT Cheshire Medical Center Keene NH -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, April 19, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joewalker <@t> rrmc.org Fri Apr 20 14:47:13 2012 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Fri Apr 20 14:47:28 2012 Subject: [Histonet] Leica Peloris Processor Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC582C87@RRMBX03.rrmc.local> Hello all, I am new to your listserve. I am wondering if anyone has experience with the Leica Peloris processor. Specifically, has anyone noticed if the higher processing temperatures has affected any IHC staining results or FISH results. Looking forward to responses, Joe W. Walker, Jr. SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 Phone: 802.747.1790 Fax:802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From Rcartun <@t> harthosp.org Fri Apr 20 15:07:16 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Apr 20 15:07:26 2012 Subject: [Histonet] Leica Peloris Processor In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC582C87@RRMBX03.rrmc.local> References: <3C2378778400AD448ADA6FD6BDB7CCCC582C87@RRMBX03.rrmc.local> Message-ID: <4F9189B4.7400.0077.1@harthosp.org> In my experience, no. Our current IHC/ISH is as good, if not better than what we were getting with our former processors. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Joe W. Walker, Jr." 4/20/2012 3:47 PM >>> Hello all, I am new to your listserve. I am wondering if anyone has experience with the Leica Peloris processor. Specifically, has anyone noticed if the higher processing temperatures has affected any IHC staining results or FISH results. Looking forward to responses, Joe W. Walker, Jr. SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 Phone: 802.747.1790 Fax:802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Fri Apr 20 15:29:30 2012 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Fri Apr 20 15:29:34 2012 Subject: [Histonet] Leica Peloris Processor In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC582C87@RRMBX03.rrmc.local> References: <3C2378778400AD448ADA6FD6BDB7CCCC582C87@RRMBX03.rrmc.local> Message-ID: We have 4 peloris processors, run many IHC and FISH stains, none of them have had any negative effects due to the increased temperatures. The increased temps aren't that high, 45 degrees for all the liquids (we use xylene) and 65 degrees for the paraffins. Good luck Patrick On Fri, Apr 20, 2012 at 12:47 PM, Joe W. Walker, Jr. wrote: > Hello all, > > I am new to your listserve. I am wondering if anyone has experience with > the Leica Peloris processor. Specifically, has anyone noticed if the > higher processing temperatures has affected any IHC staining results or > FISH results. > > Looking forward to responses, > > Joe W. Walker, Jr. SCT(ASCP)CM > Anatomical Pathology Manager > Rutland Regional Medical Center > 160 Allen Street, Rutland, VT 05701 > Phone: 802.747.1790 Fax:802.747.6525 > NEW EMAIL: joewalker@rrmc.org > www.rrmc.org > > Our Vision: To be the Best Healthcare System in New England > Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet > Recognition(r) and the Governor's Award for Performance Excellence > > This message (and any included attachments) is from Rutland Regional > Health Services and is intended only for the addressee(s). The information > contained herein may include privileged or otherwise confidential > information. Unauthorized review, forwarding, printing, copying, > distributing, or using such information is strictly prohibited and may be > unlawful. If you received this message in error, or have reason to believe > you are not authorized to receive it, please promptly delete this message > and notify the sender by e-mail. > > Thank You > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From amosbrooks <@t> gmail.com Fri Apr 20 16:46:16 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Apr 20 16:46:21 2012 Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 27 In-Reply-To: <4f919644.2959b60a.5473.5b8cSMTPIN_ADDED@mx.google.com> References: <4f919644.2959b60a.5473.5b8cSMTPIN_ADDED@mx.google.com> Message-ID: Hi, Strange issue with your Ki-67. I don't know what would do that unless it was non-specific background. Is it nuclear or cutoplasmic? If you are working in mice or other animals you could try labeling them with BRDu then detecting it with an anti BRDu antibody. Humans don't like being injected with BRDu then being sacrifices but lawyers do. Also Cleaved Caspase 3 works nicely and is probably more specific to apoptosis than Tunel which also picks up necrosis. Have a great weekend, Amos On Fri, Apr 20, 2012 at 1:00 PM, wrote: > Message: 9 > Date: Thu, 19 Apr 2012 15:07:43 -0500 > From: "Margaryan, Naira" > Subject: [Histonet] proliferation and apoptosis > To: "histonet-request@lists.utsouthwestern.edu" > > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > C1BA93040C6B9A4A8D84878F93FEC36A0AB7D0E9F1@CMHEXCC01MBX.childrensmemorial.org > > > > Content-Type: text/plain; charset="us-ascii" > > Hi histonetters, > > I am looking for the good markers to detect (separately) proliferation and > apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic > bodies as well as proliferated cells; and the tunnel assay shows both > apoptotic body and proliferation. > > Any suggestions for the FFPE tissue and the HRP protocol are appreciated. > > Thanks in advance, > Naira > From brian <@t> prometheushealthcare.com Sun Apr 22 09:32:54 2012 From: brian <@t> prometheushealthcare.com (Brian-Prometheus) Date: Sun Apr 22 09:32:59 2012 Subject: [Histonet] National Medical Laboratory Professionals Week Message-ID: <00e101cd2094$ce84c9f0$6b8e5dd0$@com> National Medical Laboratory Professionals week is an annual celebration of the medical laboratory professionals and pathologists who play a vital role in every aspect of health care. Lab Week is a time to honor the more than 300,000 medical laboratory professionals around the country who perform and interpret more than 10 billion laboratory tests in the United States each year. Thank you to all lab professionals! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From chak_bou <@t> yahoo.com Sun Apr 22 21:20:01 2012 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Sun Apr 22 21:20:05 2012 Subject: [Histonet] SMAD4 Message-ID: <1335147601.53221.YahooMailClassic@web161805.mail.bf1.yahoo.com> Hello histonet, Does anyone uses SMAD4 antibody? If so, can you tell where can I find a good one? Can you also share the staining protocol? dilution? epitope retrieval? Your help will be appreciated so much. Thank you. Chakib Boussahmain Histotech From beth.villarreal <@t> novartis.com Mon Apr 23 05:31:30 2012 From: beth.villarreal <@t> novartis.com (Villarreal, Beth) Date: Mon Apr 23 05:31:45 2012 Subject: [Histonet] anti-Cy3 for FFPE Message-ID: Does anyone know of an antibody for Cy3 that works on FFPE tissue? Many thanks, Beth Beth Villarreal Scientist I Novartis Institutes for BioMedical Research, Inc. 300 Technology Square Cambridge, MA 02139 USA Phone +1 617 8714725 Fax +1 N.A. beth.villarreal@novartis.com www.novartis.com From Albert.Santiago <@t> uphs.upenn.edu Mon Apr 23 07:23:33 2012 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Mon Apr 23 07:23:22 2012 Subject: [Histonet] GMS on toenails In-Reply-To: References: Message-ID: <63C408E65F57294790C8CFA2150A647524DECD@uphmasphi013.UPHS.PENNHEALTH.PRV> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, April 19, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas Hello Allison, we do PAS and GMS on toenails just about every day since we have a nail clinic in our department. First we dip our slides in Stay On solution (SurgiPath) and allow them to dry then we soak the faced nail block in 4% Ammonium Hydroxide for 45-60 minutes before we cut a ribbon and place on slides. We let the slides sit in a 70 degree oven for about 45 minutes, we Deparaffinize, hydrate to DH2O and we stain on the Artisan Special Stain stainer. Works every time. If it keeps falling off you might need to do it manually. Good Luck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, April 21, 2012 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 101, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Histonet Digest, Vol 101, Issue 27 (Eric Tambutte) 2. Re: Pinning Specimens (Bob Richmond) 3. Immunofluorescence (Thotakura, Anil Kumar) 4. RE: GMS on Toenail (Britton, Josette C) 5. Leica Peloris Processor (Joe W. Walker, Jr.) 6. Re: Leica Peloris Processor (Richard Cartun) 7. Re: Leica Peloris Processor (Patrick Laurie) 8. Re: Histonet Digest, Vol 101, Issue 27 (Amos Brooks) ---------------------------------------------------------------------- Message: 1 Date: Fri, 20 Apr 2012 19:03:08 +0200 From: Eric Tambutte Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 27 To: Message-ID: <1083480164@s15272523.onlinehome-server.info> Bonjour, Je suis absent du laboratoire jusqu'au jeudi 03 mai 2012. Je vous r?pondrai le plus rapidement possible. Eric Tambutt? Thank you for your mail. I will be out of office till May 03rd 2012. I will respond to your e-mail as soon as possible. Thank you for your understanding. Best regards Eric Tambutt? ------------------------------ Message: 2 Date: Fri, 20 Apr 2012 13:20:15 -0400 From: Bob Richmond Subject: [Histonet] Re: Pinning Specimens To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 About pinning specimens so they fix flat: Margaret Horne notes the use of dental wax. I've used it to make small "boats" for the rather exacting procedure of pinning muscle biopsy specimens for electron microscopy. Here a 1 to 2 mm bundle of longitudinal fibers has to be gently stretched to its resting length, pinned in the "boat", and promptly fixed in glutaraldehyde. One technologist noted that she had a young pathologist who was "wet behind the ears" and wanted to pin specimens, and was looking to the older pathologists to bring him in line to The Way We've Always Done It. I try to remain "wet behind the ears", though after nearly 50 years of occasionally pinning specimens I find myself a little dry. We continue to lose skills in the gross room. Today I'm working with a service that has almost no ability to handle calcified tissue - I need to go to the hardware store and buy a hacksaw to replace the worn-out Satterlee saw, which I think was gathered up off a Civil War battlefield. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Fri, 20 Apr 2012 18:01:17 +0000 From: "Thotakura, Anil Kumar" Subject: [Histonet] Immunofluorescence To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Dear All, Can some one please send the protocol for immunofluorescence, I want to stain for FITC CD45.1 on mice spleens. Thank you very much for your help. Many Thanks, Anil kumar. ------------------------------ Message: 4 Date: Fri, 20 Apr 2012 14:54:29 -0400 From: "Britton, Josette C" Subject: RE: [Histonet] GMS on Toenail To: "Scott, Allison D" , Message-ID: Content-Type: text/plain; charset="us-ascii" The way we got around that problem was to do a manual PAS stain instead! Josie Britton HT Cheshire Medical Center Keene NH -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, April 19, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 20 Apr 2012 19:47:13 +0000 From: "Joe W. Walker, Jr." Subject: [Histonet] Leica Peloris Processor To: "histonet@lists.utsouthwestern.edu" Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC582C87@RRMBX03.rrmc.local> Content-Type: text/plain; charset="us-ascii" Hello all, I am new to your listserve. I am wondering if anyone has experience with the Leica Peloris processor. Specifically, has anyone noticed if the higher processing temperatures has affected any IHC staining results or FISH results. Looking forward to responses, Joe W. Walker, Jr. SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 Phone: 802.747.1790 Fax:802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You ------------------------------ Message: 6 Date: Fri, 20 Apr 2012 16:07:16 -0400 From: "Richard Cartun" Subject: Re: [Histonet] Leica Peloris Processor To: "histonet@lists.utsouthwestern.edu" , "Jr. Joe W. Walker" Message-ID: <4F9189B4.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII In my experience, no. Our current IHC/ISH is as good, if not better than what we were getting with our former processors. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Joe W. Walker, Jr." 4/20/2012 3:47 PM >>> Hello all, I am new to your listserve. I am wondering if anyone has experience with the Leica Peloris processor. Specifically, has anyone noticed if the higher processing temperatures has affected any IHC staining results or FISH results. Looking forward to responses, Joe W. Walker, Jr. SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 Phone: 802.747.1790 Fax:802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 20 Apr 2012 13:29:30 -0700 From: Patrick Laurie Subject: Re: [Histonet] Leica Peloris Processor To: "Joe W. Walker, Jr." Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We have 4 peloris processors, run many IHC and FISH stains, none of them have had any negative effects due to the increased temperatures. The increased temps aren't that high, 45 degrees for all the liquids (we use xylene) and 65 degrees for the paraffins. Good luck Patrick On Fri, Apr 20, 2012 at 12:47 PM, Joe W. Walker, Jr. wrote: > Hello all, > > I am new to your listserve. I am wondering if anyone has experience with > the Leica Peloris processor. Specifically, has anyone noticed if the > higher processing temperatures has affected any IHC staining results or > FISH results. > > Looking forward to responses, > > Joe W. Walker, Jr. SCT(ASCP)CM > Anatomical Pathology Manager > Rutland Regional Medical Center > 160 Allen Street, Rutland, VT 05701 > Phone: 802.747.1790 Fax:802.747.6525 > NEW EMAIL: joewalker@rrmc.org > www.rrmc.org > > Our Vision: To be the Best Healthcare System in New England > Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet > Recognition(r) and the Governor's Award for Performance Excellence > > This message (and any included attachments) is from Rutland Regional > Health Services and is intended only for the addressee(s). The information > contained herein may include privileged or otherwise confidential > information. Unauthorized review, forwarding, printing, copying, > distributing, or using such information is strictly prohibited and may be > unlawful. If you received this message in error, or have reason to believe > you are not authorized to receive it, please promptly delete this message > and notify the sender by e-mail. > > Thank You > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com ------------------------------ Message: 8 Date: Fri, 20 Apr 2012 17:46:16 -0400 From: Amos Brooks Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 27 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, Strange issue with your Ki-67. I don't know what would do that unless it was non-specific background. Is it nuclear or cutoplasmic? If you are working in mice or other animals you could try labeling them with BRDu then detecting it with an anti BRDu antibody. Humans don't like being injected with BRDu then being sacrifices but lawyers do. Also Cleaved Caspase 3 works nicely and is probably more specific to apoptosis than Tunel which also picks up necrosis. Have a great weekend, Amos On Fri, Apr 20, 2012 at 1:00 PM, wrote: > Message: 9 > Date: Thu, 19 Apr 2012 15:07:43 -0500 > From: "Margaryan, Naira" > Subject: [Histonet] proliferation and apoptosis > To: "histonet-request@lists.utsouthwestern.edu" > > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > C1BA93040C6B9A4A8D84878F93FEC36A0AB7D0E9F1@CMHEXCC01MBX.childrensmemorial.org > > > > Content-Type: text/plain; charset="us-ascii" > > Hi histonetters, > > I am looking for the good markers to detect (separately) proliferation and > apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic > bodies as well as proliferated cells; and the tunnel assay shows both > apoptotic body and proliferation. > > Any suggestions for the FFPE tissue and the HRP protocol are appreciated. > > Thanks in advance, > Naira > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 101, Issue 28 ***************************************** The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From mw <@t> personifysearch.com Mon Apr 23 08:07:47 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Mon Apr 23 08:07:56 2012 Subject: [Histonet] Atlanta Based Field IHC Tech - Great Package!! Message-ID: <97245794210540ded5200aaaecb58ce0@mail.gmail.com> Good Morning, We are searching for a Field Based IHC Technician needed in Atlanta to join a global leading manufacturer of Histology products. Perfect for someone looking to break out of the lab and into the field!! Contact mw@personifysearch.com Our client is growing at a rapid pace and has a Field Support Specialist opportunity based in Atlanta to serve as the technical expert on IHC. This position offers an extremely competitive package and opportunity for career growth. If you or anyone you may know would be interested please contact me at mw@personifysearch.com or 800.875.6188 ext. 103 to learn more! Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From jstaruk <@t> masshistology.com Mon Apr 23 08:44:35 2012 From: jstaruk <@t> masshistology.com (jstaruk) Date: Mon Apr 23 08:44:44 2012 Subject: [Histonet] GMS on toenails In-Reply-To: <63C408E65F57294790C8CFA2150A647524DECD@uphmasphi013.UPHS.PENNHEALTH.PRV> References: <63C408E65F57294790C8CFA2150A647524DECD@uphmasphi013.UPHS.PENNHEALTH.PRV> Message-ID: <023501cd2157$3963b700$ac2b2500$@masshistology.com> We soak toenail specimens in 4% ammonia hydroxide the day before (before processing). After embedding, the nails are immediately sectioned and mounted on slides which were previously dipped in 5% gelatin. An H&E, PAS and GMS is done on each nail specimen. This procedure speeds up the overall turn-around time and prevents sections from falling off. Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santiago, Albert Sent: Monday, April 23, 2012 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on toenails From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, April 19, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas Hello Allison, we do PAS and GMS on toenails just about every day since we have a nail clinic in our department. First we dip our slides in Stay On solution (SurgiPath) and allow them to dry then we soak the faced nail block in 4% Ammonium Hydroxide for 45-60 minutes before we cut a ribbon and place on slides. We let the slides sit in a 70 degree oven for about 45 minutes, we Deparaffinize, hydrate to DH2O and we stain on the Artisan Special Stain stainer. Works every time. If it keeps falling off you might need to do it manually. Good Luck From aajl7979 <@t> hotmail.com Mon Apr 23 08:44:40 2012 From: aajl7979 <@t> hotmail.com (Amanda L) Date: Mon Apr 23 08:44:47 2012 Subject: FW: [Histonet] Part Time Job Opening, Springfield, MA In-Reply-To: References: Message-ID: > From: aajl7979@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Fri, 20 Apr 2012 09:10:08 -0400 > Subject: [Histonet] Part Time Job Opening, Springfield, MA > > > Please Send Resume for: > > Part Time opportunity for a Histotechnican. > > Duties and responsibilities include: > ? Under general supervision performs routine and non-routine activities involved in the preparation of slides, for microscopic evaluation by pathologist(s) according to policies and procedures > ? Process paperwork associated with accessioning and reporting. > ? Eligible to gross per CLIA requirements. > ? Ensure proper tissue processing. > ? Embed processed tissue in paraffin > ? Prepare slides for routine staining. > ? Perform microtomy of embedded tissue > ? Perform coverslipping of stained slides either manually or automated. > ? Perform filing of finished blocks and slides. > ? Perform routine maintenance and cleaning of equipment and troubleshoot minor equipment failures. Document remedial actions such as repairs or repeated tests. > ? Adhere to laboratory's quality control policies, and document all quality control activities. > ? Ensure all corporate safety, quality control and quality assurance standards are met. > ? Ensure compliance with all local, federal, CLIA and CAP regulations. > ? Maintain a clean and well-organized work area. > ? Clinical Chemistry & other duties, as assigned by supervisor. > > Minimum Requirements Include: > ? AA or AS degree or equivalent training and experience , CLIA approved for grossing specimens > ? HT (ASCP) or HTL (ASCP) eligible OR five years of full time experience in the last 10 years. > ? Work Experience: 1-3 years experience as a Histotechnician in an anatomic pathology laboratory. > ? License for Clinical Laboratory Technician or Technologist license. > ***Note this is a part time position also with coverage for vacation > > Thanks! _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Mon Apr 23 09:23:27 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Apr 23 09:23:40 2012 Subject: [Histonet] Who pays ...... Message-ID: <4F952D9E.7400.0077.1@harthosp.org> for the express shipping when another medical institution requests a patient's pathology slides and/or unstains or paraffin block for additional testing? In my opinion, the requesting institution should provide a FedEx, UPS or other account number for this. After all, they will be billing the patient's insurance for the consult and/or special testing that is to be performed. This is a major cost to our Department that, as you know, cannot be billed to insurance. I have instructed our staff to ask for an account number; many facilities supply one, others do not. What do you do at your facility? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From Joyce.Weems <@t> emoryhealthcare.org Mon Apr 23 09:59:45 2012 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Apr 23 10:00:05 2012 Subject: [Histonet] Who pays ...... In-Reply-To: <4F952D9E.7400.0077.1@harthosp.org> References: <4F952D9E.7400.0077.1@harthosp.org> Message-ID: We ask the requesting facility to pay shipping as well. Usually they comply. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Monday, April 23, 2012 10:23 AM To: Histonet Subject: [Histonet] Who pays ...... for the express shipping when another medical institution requests a patient's pathology slides and/or unstains or paraffin block for additional testing? In my opinion, the requesting institution should provide a FedEx, UPS or other account number for this. After all, they will be billing the patient's insurance for the consult and/or special testing that is to be performed. This is a major cost to our Department that, as you know, cannot be billed to insurance. I have instructed our staff to ask for an account number; many facilities supply one, others do not. What do you do at your facility? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From tgenade <@t> gmail.com Mon Apr 23 10:06:22 2012 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Mon Apr 23 10:06:30 2012 Subject: [Histonet] neutral buffered formalin fixed tissue and the Golgi-Cox stain Message-ID: Hello, I'm working with very small fish brains and would like to perform the modified Golgi-Cox stain of http://www.ncbi.nlm.nih.gov/pubmed/21228908 . As my area of interest is near the edge of the tissue I need to coat in egg yolk or gelatine to prevent the dark crust which forms on the tissue (is there another way?). Manipulating my tiny brains in this way in a raw unfixed state is likely to cause significant tissue damage. I have found one article where Golgi-Cox was used on neutral buffered formalin (NBF) fixed tissue (the entire pig brain, http://www.ncbi.nlm.nih.gov/pubmed/2459816) but I can't get hold of the article to actually see how this turned out. Has anyone had any experience using the Golgi-Cox stain on NBF tissue? Where there any problems? (I don't want to kill fish for nothing.) On a related matter, anyone find any faults with the protocol/solutions at http://www.funjournal.org/images/stories/downloads/2011_Volume_10_Issue_1/wright_10_1_a85_a87.pdf ? Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From Timothy.Morken <@t> ucsfmedctr.org Mon Apr 23 12:31:27 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Apr 23 12:31:32 2012 Subject: [Histonet] Barcoding specimen tracking, lessons you learned Message-ID: <8D7C2D242DBD45498006B21122072BF8AFA967D8@MCINFRWEM003.ucsfmedicalcenter.org> To anyone who has implemented a barcoding/specimen tracking system in your lab. What lessons did you learn that would make it easier if you did it over? We're starting the process and I would like to get some input on things to look out for! Thanks for any info and comments! Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org From silvinamolinuevo <@t> yahoo.com.ar Mon Apr 23 14:20:40 2012 From: silvinamolinuevo <@t> yahoo.com.ar (Silvina Molinuevo) Date: Mon Apr 23 14:20:50 2012 Subject: [Histonet] Glycosamineglycans quantitation Message-ID: <1335208840.85211.YahooMailNeo@web113614.mail.gq1.yahoo.com> Hi histonetters'!? Does anyone know the difference in glycosamineglycans quantification with 1,9-dimethyl-methylene blue or alcian blue (pH 2.5)? Thank you all Silvina From pathlocums <@t> gmail.com Mon Apr 23 14:23:37 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Mon Apr 23 14:23:41 2012 Subject: [Histonet] Barcoding specimen tracking, lessons you learned Message-ID: <-2850437400662915902@unknownmsgid> See Rich Pucci at UCSF Pathology. He would be a great resource. Sent from my Windows Phone From: Morken, Timothy Sent: 4/23/2012 10:31 AM To: Histonet Subject: [Histonet] Barcoding specimen tracking, lessons you learned To anyone who has implemented a barcoding/specimen tracking system in your lab. What lessons did you learn that would make it easier if you did it over? We're starting the process and I would like to get some input on things to look out for! Thanks for any info and comments! Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ihcman2010 <@t> hotmail.com Mon Apr 23 15:19:08 2012 From: ihcman2010 <@t> hotmail.com (Glen Dawson) Date: Mon Apr 23 15:19:12 2012 Subject: [Histonet] Qualifications for grossing In-Reply-To: <1335208840.85211.YahooMailNeo@web113614.mail.gq1.yahoo.com> References: <1335208840.85211.YahooMailNeo@web113614.mail.gq1.yahoo.com> Message-ID: All, Can a histotech perform GROSSING if he/she has an associate's degree in Histotechnology from an accredited institution (Argosy in MN)? Any help would be appreciated. Thank-you, Glen Dawson BS, HT(ASCP) & QIHC Histology Technical Specialist Mercy Health System Janesville, WI From Rcartun <@t> harthosp.org Mon Apr 23 17:30:32 2012 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Apr 23 17:30:40 2012 Subject: [Histonet] Biospecimen collection References: <4F959F680200007700037C88@gwmail3.harthosp.org> <4F959FC80200007700037C8B@gwmail3.harthosp.org> Message-ID: <4F959FC8.7400.0077.0@harthosp.org> This inquiry applies to those of you collecting human tissue for research. I am being asked to develop pricing for the following activities regarding collection of biospecimens from surgery, image-guided biopsies, endoscopic biopsies, bone marrow biopsies, and body fluids: 1. Collection of fresh tissue and freezing in liquid nitrogen. 2. Collection of fresh tissue and placement in tissue culture media. 3. Preparation of one paraffin block containing formalin-fixed tissue. 4. The preparation of one unstained slide from a paraffin block. I would appreciate hearing from those of you who might already have charges established for these activities. Thank you very much for your time. Happy "National Medical Laboratory Professionals Week" to all! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From modz9636 <@t> gmail.com Mon Apr 23 17:40:10 2012 From: modz9636 <@t> gmail.com (M.O.) Date: Mon Apr 23 17:40:13 2012 Subject: [Histonet] Histology Cassettes - Large or Extra Large Message-ID: Hello all, I am looking for extra large histology cassettes that will hold specimens that are a max. 20mm thick (high). Is there such a thing? Where would I find these larger cassettes? Thank you, Merissa From wbenton <@t> cua.md Mon Apr 23 18:26:41 2012 From: wbenton <@t> cua.md (Walter Benton) Date: Mon Apr 23 18:30:17 2012 Subject: [Histonet] Histology Cassettes - Large or Extra Large In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD92C2416C3EA@CUAEXH1.GCU-MD.local> Cassettes used to process eyes should do the trick. Fisher Scientific* Tissue Path* MACROSETTE* Processing/Embedding Cassettes Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of M.O. [modz9636@gmail.com] Sent: Monday, April 23, 2012 6:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Cassettes - Large or Extra Large Hello all, I am looking for extra large histology cassettes that will hold specimens that are a max. 20mm thick (high). Is there such a thing? Where would I find these larger cassettes? Thank you, Merissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From pathlocums <@t> gmail.com Mon Apr 23 18:32:34 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Mon Apr 23 18:32:38 2012 Subject: [Histonet] Qualifications for grossing In-Reply-To: References: <1335208840.85211.YahooMailNeo@web113614.mail.gq1.yahoo.com> Message-ID: Glen, Below are the requirements for high complexity testing, as outline by CLIA. You can reference the CLIA '88 ruling, specifically look at Subpart M, Section 493.1489 The requirements are weak, to say the least. I am not alone in the opinion that just because CLIA allows it, it is not necessarily appropriate for the minimum qualified person to be grossing certain specimens. Having someone other than an M.D., or ASCP certified PA do anything larger than a skin shave is not good medicine. But, in answer to your question - yes, the government allows inadequately trained personnel to perform high complexity testing. Sec. 493.1489 Standard; Testing personnel qualifications. Each individual performing high complexity testing must-- (a) Possess a current license issued by the State in which the laboratory is located, if such licensing is required; and (b) Meet one of the following requirements: (1) Be a doctor of medicine, doctor of osteopathy, or doctor of podiatric medicine licensed to practice medicine, osteopathy, or podiatry in the State in which the laboratory is located or have earned a doctoral, master's or bachelor's degree in a chemical, physical, biological or clinical laboratory science, or medical technology from an accredited institution; (2)(i) Have earned an associate degree in a laboratory science, or medical laboratory technology from an accredited institution or-- (ii) Have education and training equivalent to that specified in paragraph (b)(2)(i) of this section that includes-- (A) At least 60 semester hours, or equivalent, from an accredited institution that, at a minimum, include either-- (1) 24 semester hours of medical laboratory technology courses; or (2) 24 semester hours of science courses that include-- (i) Six semester hours of chemistry; (ii) Six semester hours of biology; and (iii) Twelve semester hours of chemistry, biology, or medical laboratory technology in any combination; and (B) Have laboratory training that includes either of the following: (1) Completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS. (This training may be included in the 60 semester hours listed in paragraph (b)(2)(ii)(A) of this section.) (2) At least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. (3) Have previously qualified or could have qualified as a technologist under Sec. 493.1491 on or before February 28, 1992 On Mon, Apr 23, 2012 at 1:19 PM, Glen Dawson wrote: > > All, > > Can a histotech perform GROSSING if he/she has an associate's degree in > Histotechnology from an accredited institution (Argosy in MN)? > > Any help would be appreciated. > > Thank-you, > > Glen Dawson BS, HT(ASCP) & QIHC > Histology Technical Specialist > Mercy Health System > Janesville, WI > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From mw <@t> personifysearch.com Tue Apr 24 08:04:48 2012 From: mw <@t> personifysearch.com (Matt Ward) Date: Tue Apr 24 08:04:57 2012 Subject: [Histonet] New Position Alert - Applications Support Specialists 4 Locations!! Message-ID: Good Morning Histonet, Our client is a global leader in manufacturing histology products has recently opened 4 new Application Support Specialist positions. Our client is looking for Histotechs who would be interested in working in the field and enjoy training and interacting with customers. Locations include: NJ/NY/PA (Northeast Region) NC/Charlotte (Southeast Region) Atlanta (IHC Specialist) Southern CA (Western Region) The positions include a base salary + bonus/commissions and great benefits. These are expansion opportunities and they offer a great potential for career growth. If you or anyone you may know would be interested in learning more, please contact me at mw@personifysearch.com or 800.875.6188 ext. 103 Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From Laurie <@t> blufrogpath.com Tue Apr 24 09:23:38 2012 From: Laurie <@t> blufrogpath.com (Laurie@blufrogpath.com) Date: Tue Apr 24 09:23:47 2012 Subject: [Histonet] Hazardous Waste Labeling Message-ID: <20120424072338.295dc6182df7e5cbb4f32bc101c30dcc.78b3c225b7.wbe@email15.secureserver.net> Can someone tell me the requirements for labeling hazardous waste containers and the reference source? Than Laurie Colbert From mike <@t> pathview.com Tue Apr 24 10:52:13 2012 From: mike <@t> pathview.com (Michael Mihalik) Date: Tue Apr 24 10:52:24 2012 Subject: [Histonet] LIS questions In-Reply-To: <1334773885.90033.YahooMailNeo@web125805.mail.ne1.yahoo.com> References: <1334687386.74294.YahooMailNeo@web125801.mail.ne1.yahoo.com> <1334773885.90033.YahooMailNeo@web125805.mail.ne1.yahoo.com> Message-ID: <02fa01cd2232$38a7e990$a9f7bcb0$@pathview.com> Good morning, I?d like to take up the issue of server vs cloud in Kelly?s original email. I have some thoughts that I?d like to share, but more importantly, I?d like to hear other people?s opinions. This is going to be a continuing ?hot topic? in the LIS world for years to come and I think it would be nice to have some sort of list for people to start thinking about. I am aware that traditionally there has not been a lot of LIS involvement in the histology laboratory, but with the advent of 'barcode tracking' and new quality initiatives, I am sure that this is about to change. Server Pros: 1. More control ? you know where your data resides and you have full control over it. 2. Ostensibly faster, because you?re just sharing your data traffic internally, not across the world web. 3. We know this model works in both low and large volume operations. Cons: 1. You?re responsible for maintaining the server ? operating system patches which occur every few months it seems and daily backups. 2. Cost associated with the server(s) ? Often times there will be 2 servers ? production and development, plus there is a license fee. Bottom line, think around 10, 000 to 20,000. You also need a ?place? to put the servers. Cloud Pros: 1. A user can get to the system from everywhere -- great if you don?t have an infrastructure already in place. 2. No hardware costs for the server, but I would imagine that there is some fee hidden somewhere ? nothing in life is free, right? Cons: 1. No control over your data. It can reside anywhere in the world and who knows how the local laws protect your data. If the data resides in the US, that?s less of a concern. With less control, you never really know if your backups are occurring or not. 2. Because you?re on the web, the potential exists for a slower system. This is probably not as important when a pathologist is signing out a case, but depending on the LIS, it could be a big problem, if there is a lot of user interaction. For instance, anything to do with blocks or slides which can be numerous and require rapid processing, could be an issue. In my own experience, I?ve waited on a ?web page refresh? for several seconds from time to time. If my specials are due out at 10 a.m. and it?s 9:45, I don?t have time for a slow connection. 3. With current technology, instrument interfaces can be difficult because they require more of a realtime or ?very fast? response. 4. Does anyone know of a large volume lab that uses a web based LIS where the LIS requires quick response time? 5. What happens if I change from a web based LIS. I ?assume? I don?t get access to my data unless I continue to pay some sort of fee. With a server based system, I can stop paying maintenance, but I can still access my data. What do you all think? Do you disagree with the list? Do you have more to add? Bottom line, I think for me, and remember, I?m an LIS vendor myself, system functionality would still be a priority as far as system selection is concerned. The ?feel of the company? would be my next critical concern, and then I would think about cost and technology. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd Sent: Wednesday, April 18, 2012 11:31 AM To: histonet Subject: [Histonet] LIS questions ? Is anyone out there familiar with any of the following LIS systems, if so, what are your thoughts??? AIM Path Software systems, Wavefront Software, WinSURGE, CSS LIS ? What are the opinions out there?for a server based system versus a cloud based?? Thanks! Kelly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Tue Apr 24 11:27:47 2012 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Apr 24 11:27:56 2012 Subject: [Histonet] LIS questions In-Reply-To: <02fa01cd2232$38a7e990$a9f7bcb0$@pathview.com> References: <1334687386.74294.YahooMailNeo@web125801.mail.ne1.yahoo.com>, <1334773885.90033.YahooMailNeo@web125805.mail.ne1.yahoo.com>, <02fa01cd2232$38a7e990$a9f7bcb0$@pathview.com> Message-ID: When we start talking about the issues associated w/ server vs. cloud in healthcare, I think discussion needs to start w/ and be centered on capability of compliance and enforcement, as it relates to HITECH Act, HIPPA and delivery of ePHI. There is a complexity added as more health information exchanges are developed and access to data is expanded and the need to develop complex and sound Business Associate Agreements. In my mind, the idea of more access and/or ease of access must be directly related to the ability to control and provide security of access. I believe this very complex issue and process will rely less on the needs of the end user, Histotechnicians/Histotechnologists and more on the need to meet HITECH requirements and very secure delivery. This is a very complex IT issue and maybe not best discussed on the Histonet w/ technical personnel with limited IT knowledge. William DeSalvo, BS, HTL(ASCP) > From: mike@pathview.com > To: kdboydhisto@yahoo.com; Histonet@lists.utsouthwestern.edu > Date: Tue, 24 Apr 2012 08:52:13 -0700 > Subject: RE: [Histonet] LIS questions > CC: > > Good morning, > > I?d like to take up the issue of server vs cloud in Kelly?s original email. > I have some thoughts that I?d like to share, but more importantly, I?d like > to hear other people?s opinions. This is going to be a continuing ?hot > topic? in the LIS world for years to come and I think it would be nice to > have some sort of list for people to start thinking about. I am aware that > traditionally there has not been a lot of LIS involvement in the histology > laboratory, but with the advent of 'barcode tracking' and new quality > initiatives, I am sure that this is about to change. > > Server > Pros: > 1. More control ? you know where your data resides and you have full > control over it. > 2. Ostensibly faster, because you?re just sharing your data traffic > internally, not across the world web. > 3. We know this model works in both low and large volume operations. > Cons: > 1. You?re responsible for maintaining the server ? operating system > patches which occur every few months it seems and daily backups. > 2. Cost associated with the server(s) ? Often times there will be 2 > servers ? production and development, plus there is a license fee. Bottom > line, think around 10, 000 to 20,000. You also need a ?place? to put the > servers. > > > Cloud > Pros: > 1. A user can get to the system from everywhere -- great if you don?t > have an infrastructure already in place. > 2. No hardware costs for the server, but I would imagine that there is > some fee hidden somewhere ? nothing in life is free, right? > Cons: > 1. No control over your data. It can reside anywhere in the world and > who knows how the local laws protect your data. If the data resides in the > US, that?s less of a concern. With less control, you never really know if > your backups are occurring or not. > 2. Because you?re on the web, the potential exists for a slower system. > This is probably not as important when a pathologist is signing out a case, > but depending on the LIS, it could be a big problem, if there is a lot of > user interaction. For instance, anything to do with blocks or slides which > can be numerous and require rapid processing, could be an issue. In my own > experience, I?ve waited on a ?web page refresh? for several seconds from > time to time. If my specials are due out at 10 a.m. and it?s 9:45, I don?t > have time for a slow connection. > 3. With current technology, instrument interfaces can be difficult > because they require more of a realtime or ?very fast? response. > 4. Does anyone know of a large volume lab that uses a web based LIS > where the LIS requires quick response time? > 5. What happens if I change from a web based LIS. I ?assume? I don?t > get access to my data unless I continue to pay some sort of fee. With a > server based system, I can stop paying maintenance, but I can still access > my data. > > What do you all think? Do you disagree with the list? Do you have more to > add? > > Bottom line, I think for me, and remember, I?m an LIS vendor myself, system > functionality would still be a priority as far as system selection is > concerned. The ?feel of the company? would be my next critical concern, and > then I would think about cost and technology. > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd > Sent: Wednesday, April 18, 2012 11:31 AM > To: histonet > Subject: [Histonet] LIS questions > > > > > > Is anyone out there familiar with any of the following LIS systems, if so, > what are your thoughts?? AIM Path Software systems, Wavefront Software, > WinSURGE, CSS LIS > > What are the opinions out there for a server based system versus a cloud > based?? > Thanks! > > > Kelly > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Apr 24 11:49:56 2012 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Apr 24 11:50:11 2012 Subject: [Histonet] LIS questions In-Reply-To: References: <1334687386.74294.YahooMailNeo@web125801.mail.ne1.yahoo.com>, <1334773885.90033.YahooMailNeo@web125805.mail.ne1.yahoo.com>, <02fa01cd2232$38a7e990$a9f7bcb0$@pathview.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E3913819D4439@IBMB7Exchange.digestivespecialists.com> Not all Histotechnicians/Histotechnologists have limited IT knowledge. There are a great number of those that have an IT team with limited knowledge of what exactly occurs in the histology lab. The more information that Histotechnicians/Histotechnologists that are actively searching for a new system are armed with the more capable they are to intelligently participate in making decisions. If we are going to limit the discussions here then we would have to start weeding out the discussions that pertain to information that only applies to a select minority. Personally I enjoy the information that has no bearing on my particular lab. It gives me information to think about even though I may never use. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Tuesday, April 24, 2012 12:28 PM To: mike@pathview.com; Kelly Boyd; histonet Subject: RE: [Histonet] LIS questions When we start talking about the issues associated w/ server vs. cloud in healthcare, I think discussion needs to start w/ and be centered on capability of compliance and enforcement, as it relates to HITECH Act, HIPPA and delivery of ePHI. There is a complexity added as more health information exchanges are developed and access to data is expanded and the need to develop complex and sound Business Associate Agreements. In my mind, the idea of more access and/or ease of access must be directly related to the ability to control and provide security of access. I believe this very complex issue and process will rely less on the needs of the end user, Histotechnicians/Histotechnologists and more on the need to meet HITECH requirements and very secure delivery. This is a very complex IT issue and maybe not best discussed on the Histonet w/ technical personnel with limited IT knowledge. William DeSalvo, BS, HTL(ASCP) > From: mike@pathview.com > To: kdboydhisto@yahoo.com; Histonet@lists.utsouthwestern.edu > Date: Tue, 24 Apr 2012 08:52:13 -0700 > Subject: RE: [Histonet] LIS questions > CC: > > Good morning, > > I'd like to take up the issue of server vs cloud in Kelly's original email. > I have some thoughts that I'd like to share, but more importantly, I'd > like to hear other people's opinions. This is going to be a continuing > 'hot topic' in the LIS world for years to come and I think it would be > nice to have some sort of list for people to start thinking about. I > am aware that traditionally there has not been a lot of LIS > involvement in the histology laboratory, but with the advent of > 'barcode tracking' and new quality initiatives, I am sure that this is about to change. > > Server > Pros: > 1. More control - you know where your data resides and you have full > control over it. > 2. Ostensibly faster, because you're just sharing your data traffic > internally, not across the world web. > 3. We know this model works in both low and large volume operations. > Cons: > 1. You're responsible for maintaining the server - operating system > patches which occur every few months it seems and daily backups. > 2. Cost associated with the server(s) - Often times there will be 2 > servers - production and development, plus there is a license fee. > Bottom line, think around 10, 000 to 20,000. You also need a 'place' > to put the servers. > > > Cloud > Pros: > 1. A user can get to the system from everywhere -- great if you don't > have an infrastructure already in place. > 2. No hardware costs for the server, but I would imagine that there is > some fee hidden somewhere - nothing in life is free, right? > Cons: > 1. No control over your data. It can reside anywhere in the world and > who knows how the local laws protect your data. If the data resides in > the US, that's less of a concern. With less control, you never really > know if your backups are occurring or not. > 2. Because you're on the web, the potential exists for a slower system. > This is probably not as important when a pathologist is signing out a > case, but depending on the LIS, it could be a big problem, if there is > a lot of user interaction. For instance, anything to do with blocks or > slides which can be numerous and require rapid processing, could be an > issue. In my own experience, I've waited on a 'web page refresh' for > several seconds from time to time. If my specials are due out at 10 > a.m. and it's 9:45, I don't have time for a slow connection. > 3. With current technology, instrument interfaces can be difficult > because they require more of a realtime or 'very fast' response. > 4. Does anyone know of a large volume lab that uses a web based LIS > where the LIS requires quick response time? > 5. What happens if I change from a web based LIS. I 'assume' I don't > get access to my data unless I continue to pay some sort of fee. With > a server based system, I can stop paying maintenance, but I can still > access my data. > > What do you all think? Do you disagree with the list? Do you have more > to add? > > Bottom line, I think for me, and remember, I'm an LIS vendor myself, > system functionality would still be a priority as far as system > selection is concerned. The 'feel of the company' would be my next > critical concern, and then I would think about cost and technology. > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: > 952.241.7369 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly > Boyd > Sent: Wednesday, April 18, 2012 11:31 AM > To: histonet > Subject: [Histonet] LIS questions > > > > > > Is anyone out there familiar with any of the following LIS systems, if > so, what are your thoughts?? AIM Path Software systems, Wavefront > Software, WinSURGE, CSS LIS > > What are the opinions out there for a server based system versus a > cloud based?? > Thanks! > > > Kelly > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Tue Apr 24 12:59:24 2012 From: mike <@t> pathview.com (Michael Mihalik) Date: Tue Apr 24 12:59:39 2012 Subject: [Histonet] LIS questions In-Reply-To: References: <1334687386.74294.YahooMailNeo@web125801.mail.ne1.yahoo.com>, <1334773885.90033.YahooMailNeo@web125805.mail.ne1.yahoo.com>, <02fa01cd2232$38a7e990$a9f7bcb0$@pathview.com> Message-ID: <036101cd2243$fd54aab0$f7fe0010$@pathview.com> William, you bring up a very good point, but I?d like to respond to your concerns this way: 1. I don?t know how my online banking security works, but It does. I have to believe that the industry and ?cloud based? LIS vendors have already solved this issue or that they will in the short term. Perhaps I am na?ve in where I place my trust, but the ?cloud? is such a big marketing thrust these days, I have to believe that momentum alone will carry this forward. 2. I guess I?m more of a functionality person than a technical person though I do have a technical degree from a pretty decent school. There are so many perspectives to this issue that I am content to confine the current thoughts to mostly functionality versus technical or legal. Still, I always try to be open to other thoughts and your commentary did just that ? thank you. 3. Finally, I think this topic is relevant to histonet because I?m hearing more and more LIS vendors differentiate themselves as cloud vs server based solutions. Since I think the LIS will play more of a role in the daily lives of histotechnologists, I think it?s a good idea to talk about this and leave some trail of discovery for future people interested in this topic. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 From: WILLIAM DESALVO [mailto:wdesalvo.cac@hotmail.com] Sent: Tuesday, April 24, 2012 9:28 AM To: mike@pathview.com; Kelly Boyd; histonet Subject: RE: [Histonet] LIS questions When we start talking about the issues associated w/ server vs. cloud in healthcare, I think discussion needs to start w/ and be centered on capability of compliance and enforcement, as it relates to HITECH Act, HIPPA and delivery of ePHI. There is a complexity added as more health information exchanges are developed and access to data is expanded and the need to develop complex and sound Business Associate Agreements. In my mind, the idea of more access and/or ease of access must be directly related to the ability to control and provide security of access. I believe this very complex issue and process will rely less on the needs of the end user, Histotechnicians/Histotechnologists and more on the need to meet HITECH requirements and very secure delivery. This is a very complex IT issue and maybe not best discussed on the Histonet w/ technical personnel with limited IT knowledge. William DeSalvo, BS, HTL(ASCP) > From: mike@pathview.com > To: kdboydhisto@yahoo.com; Histonet@lists.utsouthwestern.edu > Date: Tue, 24 Apr 2012 08:52:13 -0700 > Subject: RE: [Histonet] LIS questions > CC: > > Good morning, > > I?d like to take up the issue of server vs cloud in Kelly?s original email. > I have some thoughts that I?d like to share, but more importantly, I?d like > to hear other people?s opinions. This is going to be a continuing ?hot > topic? in the LIS world for years to come and I think it would be nice to > have some sort of list for people to start thinking about. I am aware that > traditionally there has not been a lot of LIS involvement in the histology > laboratory, but with the advent of 'barcode tracking' and new quality > initiatives, I am sure that this is about to change. > > Server > Pros: > 1. More control ? you know where your data resides and you have full > control over it. > 2. Ostensibly faster, because you?re just sharing your data traffic > internally, not across the world web. > 3. We know this model works in both low and large volume operations. > Cons: > 1. You?re responsible for maintaining the server ? operating system > patches which occur every few months it seems and daily backups. > 2. Cost associated with the server(s) ? Often times there will be 2 > servers ? production and development, plus there is a license fee. Bottom > line, think around 10, 000 to 20,000. You also need a ?place? to put the > servers. > > > Cloud > Pros: > 1. A user can get to the system from everywhere -- great if you don?t > have an infrastructure already in place. > 2. No hardware costs for the server, but I would imagine that there is > some fee hidden somewhere ? nothing in life is free, right? > Cons: > 1. No control over your data. It can reside anywhere in the world and > who knows how the local laws protect your data. If the data resides in the > US, that?s less of a concern. With less control, you never really know if > your backups are occurring or not. > 2. Because you?re on the web, the potential exists for a slower system. > This is probably not as important when a pathologist is signing out a case, > but depending on the LIS, it could be a big problem, if there is a lot of > user interaction. For instance, anything to do with blocks or slides which > can be numerous and require rapid processing, could be an issue. In my own > experience, I?ve waited on a ?web page refresh? for several seconds from > time to time. If my specials are due out at 10 a.m. and it?s 9:45, I don?t > have time for a slow connection. > 3. With current technology, instrument interfaces can be difficult > because they require more of a realtime or ?very fast? response. > 4. Does anyone know of a large volume lab that uses a web based LIS > where the LIS requires quick response time? > 5. What happens if I change from a web based LIS. I ?assume? I don?t > get access to my data unless I continue to pay some sort of fee. With a > server based system, I can stop paying maintenance, but I can still access > my data. > > What do you all think? Do you disagree with the list? Do you have more to > add? > > Bottom line, I think for me, and remember, I?m an LIS vendor myself, system > functionality would still be a priority as far as system selection is > concerned. The ?feel of the company? would be my next critical concern, and > then I would think about cost and technology. > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd > Sent: Wednesday, April 18, 2012 11:31 AM > To: histonet > Subject: [Histonet] LIS questions > > > > > > Is anyone out there familiar with any of the following LIS systems, if so, > what are your thoughts?? AIM Path Software systems, Wavefront Software, > WinSURGE, CSS LIS > > What are the opinions out there for a server based system versus a cloud > based?? > Thanks! > > > Kelly > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Tue Apr 24 13:25:08 2012 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Apr 24 13:25:21 2012 Subject: [Histonet] RE: Barcoding specimen tracking, lessons you learned In-Reply-To: <8D7C2D242DBD45498006B21122072BF8AFA967D8@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF8AFA967D8@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: Hi Tim, One thing I did not anticipate that was an unfortunate eye opener. When humans are given a scanner to read barcodes they seem to trust the technology so much that they stop reading with their eyes. We had an unfortunate incident where the wrong barcode label was placed onto a specimen container at accessioning. Keep in mind the label containing the barcode also contained a patient name and MRN. Had the next individual in the chain of events, the individual at the grossing table, read the label, she might have noticed the discrepancy. Instead, the barcode on the cassettes was for the wrong patient and of course, the slides likewise. So while I adopted barcodes to reduce the likelihood of error, this event made me feel a bit more vulnerable because once the scanners are in use, you may find that staff become so reliant on the technology that they are no longer vigilant in keeping an eye out for problems. I'm sharing this in the hope that by alerting your staff to this pitfall you can avoid experiencing this in your lab. Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, April 23, 2012 1:31 PM To: Histonet Subject: [Histonet] Barcoding specimen tracking, lessons you learned To anyone who has implemented a barcoding/specimen tracking system in your lab. What lessons did you learn that would make it easier if you did it over? We're starting the process and I would like to get some input on things to look out for! Thanks for any info and comments! Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Tue Apr 24 14:14:06 2012 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Tue Apr 24 14:14:27 2012 Subject: [Histonet] Napsin A Message-ID: Hello. I was wondering if anyone had used the NapsinA from BioCare on the Bond III and if so, would you mind sharing your protocol? Thanks in advance for any advice you can give. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2109 From mike <@t> pathview.com Tue Apr 24 14:16:13 2012 From: mike <@t> pathview.com (Michael Mihalik) Date: Tue Apr 24 14:16:35 2012 Subject: [Histonet] RE: Barcoding specimen tracking, lessons you learned In-Reply-To: References: <8D7C2D242DBD45498006B21122072BF8AFA967D8@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <03b601cd224e$b85e1760$291a4620$@pathview.com> Unfortunately (or is that fortunately?), pretty much all technology can be foiled by human beings. We've seen this scenario before as well. We tell our accession personnel that they must accession and label one case at a time, but when it comes down to it, there is no real way to FORCE people to follow a policy. This is why we have inquiry come up automatically at Gross, Transcription, and Pathology workcenters. Ostensibly, one or more people need to confirm the correct labeling of the case. ...at least that's the theory ... and of course there's that people part of the equation again. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, April 24, 2012 11:25 AM To: Morken, Timothy; Histonet Subject: [Histonet] RE: Barcoding specimen tracking, lessons you learned Hi Tim, One thing I did not anticipate that was an unfortunate eye opener. When humans are given a scanner to read barcodes they seem to trust the technology so much that they stop reading with their eyes. We had an unfortunate incident where the wrong barcode label was placed onto a specimen container at accessioning. Keep in mind the label containing the barcode also contained a patient name and MRN. Had the next individual in the chain of events, the individual at the grossing table, read the label, she might have noticed the discrepancy. Instead, the barcode on the cassettes was for the wrong patient and of course, the slides likewise. So while I adopted barcodes to reduce the likelihood of error, this event made me feel a bit more vulnerable because once the scanners are in use, you may find that staff become so reliant on the technology that they are no longer vigilant in keeping an eye out for problems. I'm sharing this in the hope that by alerting your staff to this pitfall you can avoid experiencing this in your lab. Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, April 23, 2012 1:31 PM To: Histonet Subject: [Histonet] Barcoding specimen tracking, lessons you learned To anyone who has implemented a barcoding/specimen tracking system in your lab. What lessons did you learn that would make it easier if you did it over? We're starting the process and I would like to get some input on things to look out for! Thanks for any info and comments! Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Tue Apr 24 14:56:58 2012 From: histotalk <@t> yahoo.com (David Kemler) Date: Tue Apr 24 14:57:02 2012 Subject: [Histonet] HistoTALK at the FSH Meeting in May Message-ID: <1335297418.51256.YahooMailNeo@web120601.mail.ne1.yahoo.com> Hi everyone, HistoTALK www.HistoTALK.com was invited to "tape" a few interviews?at this years 2012 Florida Society for Histotechnology Meeting at the Grand Hyatt Tampa Bay, May 17th, 18th and 19th. If you would like to be a guest on a future HistoTALK program, let me know and we can?decide on a good time for you to tape. ? If you want to attend one of the largest and best society meetings on the east coast - this is it! Check them out at www.FSHgroup.org. ? Even if you are not interested in being a guest on the show, stop by the HistoTALK "mobile" studio and say hello or catch up on all the latest! ? See you at the FSH Meeting in May, the Region VII in Phoenix, AZ in June AND the 2012 NSH Symposium / Convention in Vancouver. ? Yours, Dave From one_angel_secret <@t> yahoo.com Tue Apr 24 15:25:24 2012 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Apr 24 15:25:30 2012 Subject: [Histonet] RE: Barcoding specimen tracking, lessons you learned In-Reply-To: References: <8D7C2D242DBD45498006B21122072BF8AFA967D8@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: Vinnie. I as well found some quirks with this technology and I'm glad you brought up that it isn't perfect. I had a bog issue with the fact that you couldn't scan all your cassettes when putting on the machine as I felt that was the time errors needed to be noticed and fixed. Because finding out even the next day that a specimen is missing is not a good time. Ie. Specimen got tossed into garbage upon grossing and garbage has been incinerated. Not that I've seen this. Just that it could happen. I also noticed that errors at microtomy can still happen. Think about this, you pick up a block. Scan it and then can't get a section. You put block back in decal or on ice. You pick up next block. Whamo. You better pay attention So in essence with what ever we use technology wise it's up to us if we are paying attention and should never be complacent with our tools. Because our tools still require us to think. Kim D Sent from my iPhone On Apr 24, 2012, at 2:25 PM, "Della Speranza, Vinnie" wrote: > Hi Tim, > > One thing I did not anticipate that was an unfortunate eye opener. > > When humans are given a scanner to read barcodes they seem to trust the technology so much that they stop reading with their eyes. > > We had an unfortunate incident where the wrong barcode label was placed onto a specimen container at accessioning. Keep in mind the label containing the barcode also contained a patient name and MRN. Had the next individual in the chain of events, the individual at the grossing table, read the label, she might have noticed the discrepancy. Instead, the barcode on the cassettes was for the wrong patient and of course, the slides likewise. > > So while I adopted barcodes to reduce the likelihood of error, this event made me feel a bit more vulnerable because once the scanners are in use, you may find that staff become so reliant on the technology that they are no longer vigilant in keeping an eye out for problems. I'm sharing this in the hope that by alerting your staff to this pitfall you can avoid experiencing this in your lab. > > > Vinnie Della Speranza, MS, HTL(ASCP) > Manager for Anatomic Pathology Services > Medical University of South Carolina > 165 Ashley Avenue MSC 908 > Charleston, SC 29425 > tel. 843-792-6353 > fax. 843-792-8974 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Monday, April 23, 2012 1:31 PM > To: Histonet > Subject: [Histonet] Barcoding specimen tracking, lessons you learned > > To anyone who has implemented a barcoding/specimen tracking system in your lab. What lessons did you learn that would make it easier if you did it over? We're starting the process and I would like to get some input on things to look out for! > > Thanks for any info and comments! > > Tim Morken > Department of Pathology > UC San Francisco Medical Center > 505 Parnassus Ave, Box 1656 > Room S570 > San Francisco, CA 94132 > > (415) 353-1266 (ph) > (415) 514-3403 (fax) > tim.morken@ucsfmedctr.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Tue Apr 24 15:34:03 2012 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Apr 24 15:34:28 2012 Subject: [Histonet] RE: Barcoding specimen tracking, lessons you learned In-Reply-To: References: <8D7C2D242DBD45498006B21122072BF8AFA967D8@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: Thanks for contacting me Kim. I think that staff feel compelled to be more vigilant when manual systems are in place and may get a bit too complacent, expecting the electronic tools to think for us. we are working on developing our own tools for scanning cassettes before they are loaded into the tissue processors, but the tool is in development stage and I can't offer specifics yet, however it is supposed to go live in July. We are creating a version of CoPath that will be married to Cerner Millenium. At the grossing table, the cassettes will be scanned when the tissues are collected and held in formalin, then scanned again at the tissue processor and again at the embedding station. At embedding, a screen pops up on a monitor that will alert the tech is there are special embedding instructions. This is entirely home grown and not an off the shelf product. I share this because the scenarios you mentioned are in fact possible, I lived through the nightmare of having to search through two tractor trailer loads of red bag waste to find a missing kidney biopsy. The specimen was found, but it was the most unpleasant experience imaginable. And I survived to tell about it ! Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Tuesday, April 24, 2012 4:25 PM To: Della Speranza, Vinnie Cc: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Barcoding specimen tracking, lessons you learned Vinnie. I as well found some quirks with this technology and I'm glad you brought up that it isn't perfect. I had a bog issue with the fact that you couldn't scan all your cassettes when putting on the machine as I felt that was the time errors needed to be noticed and fixed. Because finding out even the next day that a specimen is missing is not a good time. Ie. Specimen got tossed into garbage upon grossing and garbage has been incinerated. Not that I've seen this. Just that it could happen. I also noticed that errors at microtomy can still happen. Think about this, you pick up a block. Scan it and then can't get a section. You put block back in decal or on ice. You pick up next block. Whamo. You better pay attention So in essence with what ever we use technology wise it's up to us if we are paying attention and should never be complacent with our tools. Because our tools still require us to think. Kim D Sent from my iPhone On Apr 24, 2012, at 2:25 PM, "Della Speranza, Vinnie" wrote: > Hi Tim, > > One thing I did not anticipate that was an unfortunate eye opener. > > When humans are given a scanner to read barcodes they seem to trust the technology so much that they stop reading with their eyes. > > We had an unfortunate incident where the wrong barcode label was placed onto a specimen container at accessioning. Keep in mind the label containing the barcode also contained a patient name and MRN. Had the next individual in the chain of events, the individual at the grossing table, read the label, she might have noticed the discrepancy. Instead, the barcode on the cassettes was for the wrong patient and of course, the slides likewise. > > So while I adopted barcodes to reduce the likelihood of error, this event made me feel a bit more vulnerable because once the scanners are in use, you may find that staff become so reliant on the technology that they are no longer vigilant in keeping an eye out for problems. I'm sharing this in the hope that by alerting your staff to this pitfall you can avoid experiencing this in your lab. > > > Vinnie Della Speranza, MS, HTL(ASCP) > Manager for Anatomic Pathology Services > Medical University of South Carolina > 165 Ashley Avenue MSC 908 > Charleston, SC 29425 > tel. 843-792-6353 > fax. 843-792-8974 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Monday, April 23, 2012 1:31 PM > To: Histonet > Subject: [Histonet] Barcoding specimen tracking, lessons you learned > > To anyone who has implemented a barcoding/specimen tracking system in your lab. What lessons did you learn that would make it easier if you did it over? We're starting the process and I would like to get some input on things to look out for! > > Thanks for any info and comments! > > Tim Morken > Department of Pathology > UC San Francisco Medical Center > 505 Parnassus Ave, Box 1656 > Room S570 > San Francisco, CA 94132 > > (415) 353-1266 (ph) > (415) 514-3403 (fax) > tim.morken@ucsfmedctr.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From claycal44 <@t> yahoo.com Tue Apr 24 15:46:02 2012 From: claycal44 <@t> yahoo.com (nancy lowen) Date: Tue Apr 24 15:46:12 2012 Subject: [Histonet] WNT-16 Antibody Message-ID: <1335300362.89496.YahooMailNeo@web164503.mail.gq1.yahoo.com> Hoping someone can help----- Does anyone know of a WNT-16 antibody that will work on mouse tissue for IHC? Thanks, Nancy.lowen@va.gov From dellav <@t> musc.edu Tue Apr 24 15:54:46 2012 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Apr 24 15:55:00 2012 Subject: [Histonet] RE: Barcoding specimen tracking, lessons you learned In-Reply-To: References: <8D7C2D242DBD45498006B21122072BF8AFA967D8@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: in order to protect the innocent I want to mention that the "lost" kidney biopsy that was successfully found in a mountain of red bag waste occurred at another facility and not where I am currently employed. This is one of those experiences you never forget but fortunately had a happy ending and has been the subject of workshops I've given for NSH. Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 - From billodonnell <@t> catholichealth.net Tue Apr 24 16:04:09 2012 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Apr 24 16:04:13 2012 Subject: [Histonet] RE: Barcoding specimen tracking, lessons you learned In-Reply-To: References: <8D7C2D242DBD45498006B21122072BF8AFA967D8@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <4940DF6D1C5FDF48931B6966AAEF93955706BA@chimsx08.CHI.catholichealth.net> Dumpster diving should be rare among histotechs, but I suspect we have all done it in one form or another at some point in our careers. Vinnie, yours is the most extreme senerio I recall. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, April 24, 2012 3:55 PM To: Della Speranza, Vinnie; Kim Donadio Cc: Histonet; Morken,Timothy Subject: RE: [Histonet] RE: Barcoding specimen tracking, lessons you learned in order to protect the innocent I want to mention that the "lost" kidney biopsy that was successfully found in a mountain of red bag waste occurred at another facility and not where I am currently employed. This is one of those experiences you never forget but fortunately had a happy ending and has been the subject of workshops I've given for NSH. Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue MSC 908 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 - _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From chak_bou <@t> yahoo.com Tue Apr 24 20:31:12 2012 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Tue Apr 24 20:31:16 2012 Subject: [Histonet] CD4 and CD45 antibodies Message-ID: <1335317472.68637.YahooMailClassic@web161805.mail.bf1.yahoo.com> Hello Histonetters, Does anyone uses CD4 and/or CD45 antibodies? If so, can you share the protocol? dilutions? and epitope retrieval? Thank you for your help! Appreciated Chakib Boussahmain Histotechnologist HTL From W.E.J.Hoekert <@t> olvg.nl Wed Apr 25 04:49:26 2012 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Wed Apr 25 04:50:48 2012 Subject: [Histonet] SMAD4 References: <1335147601.53221.YahooMailClassic@web161805.mail.bf1.yahoo.com> Message-ID: <1190CB05C44B13409483514729C2FC3601F84243@PAIT42.olvg.nl> Which stainer are you using? Because some clones will not work on a Venatana stainer. Willem ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Chakib Boussahmain Verzonden: ma 23-4-2012 4:20 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] SMAD4 Hello histonet, Does anyone uses SMAD4 antibody? If so, can you tell where can I find a good one? Can you also share the staining protocol? dilution? epitope retrieval? Your help will be appreciated so much. Thank you. Chakib Boussahmain Histotech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From jclark <@t> pcnm.com Wed Apr 25 09:41:13 2012 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Apr 25 09:41:52 2012 Subject: [Histonet] RE: Qualifications for grossing In-Reply-To: <20120424170509.F2E758B70FC@mx10.myoutlookonline.com> References: <20120424170509.F2E758B70FC@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E3784CB7F4B5@S10MAILD001N1.SH10.lan> David, after reading your post I was not at all surprised to see that you are a PA. I am assuming that explains your vitriol towards techs that gross. Yes, CLIA does provide the educational requirements for high complexity testing, but what on earth makes you think that a tech with the proper CLIA qualifications can gross without proper training by a pathologist? CAP requires that as well as extensive documentation of training AND a list of the specimens approved by the Lab Director that a 'non-pathologist' is allowed to gross. I'm sure you can tell that I am a Histotech with an Associates Degree and I do the grossing in my lab. I can assure you that I do a good job and if there is EVER any question regarding how to gross in a specimen I will get a pathologist. To make it clear, just because we tech's that gross do not have a masters as a pathologist assistant, we care just as much about the patients we serve as a PA does. Another point I would like to make is that very often we gross not by choice but because it is what our pathologists demand of us and they wouldn't put us there if we couldn't do the job. Believe me, when I say that I do want to get my masters as a PA, but I haven't been able to find a program that accommodates someone who is working full time and can not afford to quit to go back to school. I am currently finishing up my Bachelors, because I still want to pursue it. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ------------------------------ Message: 8 Date: Mon, 23 Apr 2012 16:32:34 -0700 From: Davide Costanzo Subject: Re: [Histonet] Qualifications for grossing To: Glen Dawson Cc: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Glen, Below are the requirements for high complexity testing, as outline by CLIA. You can reference the CLIA '88 ruling, specifically look at Subpart M, Section 493.1489 The requirements are weak, to say the least. I am not alone in the opinion that just because CLIA allows it, it is not necessarily appropriate for the minimum qualified person to be grossing certain specimens. Having someone other than an M.D., or ASCP certified PA do anything larger than a skin shave is not good medicine. But, in answer to your question - yes, the government allows inadequately trained personnel to perform high complexity testing. Sec. 493.1489 Standard; Testing personnel qualifications. Each individual performing high complexity testing must-- (a) Possess a current license issued by the State in which the laboratory is located, if such licensing is required; and (b) Meet one of the following requirements: (1) Be a doctor of medicine, doctor of osteopathy, or doctor of podiatric medicine licensed to practice medicine, osteopathy, or podiatry in the State in which the laboratory is located or have earned a doctoral, master's or bachelor's degree in a chemical, physical, biological or clinical laboratory science, or medical technology from an accredited institution; (2)(i) Have earned an associate degree in a laboratory science, or medical laboratory technology from an accredited institution or-- (ii) Have education and training equivalent to that specified in paragraph (b)(2)(i) of this section that includes-- (A) At least 60 semester hours, or equivalent, from an accredited institution that, at a minimum, include either-- (1) 24 semester hours of medical laboratory technology courses; or (2) 24 semester hours of science courses that include-- (i) Six semester hours of chemistry; (ii) Six semester hours of biology; and (iii) Twelve semester hours of chemistry, biology, or medical laboratory technology in any combination; and (B) Have laboratory training that includes either of the following: (1) Completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS. (This training may be included in the 60 semester hours listed in paragraph (b)(2)(ii)(A) of this section.) (2) At least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. (3) Have previously qualified or could have qualified as a technologist under Sec. 493.1491 on or before February 28, 1992 On Mon, Apr 23, 2012 at 1:19 PM, Glen Dawson wrote: > > All, > > Can a histotech perform GROSSING if he/she has an associate's degree > in Histotechnology from an accredited institution (Argosy in MN)? > > Any help would be appreciated. > > Thank-you, > > Glen Dawson BS, HT(ASCP) & QIHC > Histology Technical Specialist > Mercy Health System > Janesville, WI > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From pathlocums <@t> gmail.com Wed Apr 25 11:34:29 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Wed Apr 25 11:34:39 2012 Subject: [Histonet] RE: Qualifications for grossing In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3784CB7F4B5@S10MAILD001N1.SH10.lan> References: <20120424170509.F2E758B70FC@mx10.myoutlookonline.com> <0494A7D4E8CC254EA2FB81464982E3784CB7F4B5@S10MAILD001N1.SH10.lan> Message-ID: Joanna, I wanted to take an opportunity to explain my, and most of my colleagues, feelings about CLIA '88 with respect to grossing standards. But I want to start by stating that this goes both ways, I also do not feel it is appropriate for an ASCP certified PA to be performing Immunohistochemistry, or other stains in the lab. Both histotechnicians (ologists) and PA's have a very clear role in the pathology laboratory. Both have very different training programs. Both HT's and PA's should be protected by law, and rules/regulations for each should be clear. One is not better than the other, and I certainly hope you do not think I have an opinion different from that. Both are highly qualified individuals in their area of expertise. In many states, and I will use Florida as an example because that is what I am familiar with, there are clear definitions in the law as to whom can perform what tasks. In the State of Florida, a PA (regardless of training level) is not to perform frozen sections. That State only allows Pathologists and HT's to cut a frozen. This is the result of much effort put in to changing those rules by the HT's in Florida. Clearly they saw PA's as a threat to their job, and took action. Not a problem, I am happy to let them do the frozen sections. What was it about cutting a frozen section that the HT's thought a PA could not handle? I do not know, but nonetheless they reacted. Certainly PA's are heavily trained in how to cut a frozen section, and it is generally considered our responsibility in most places in the US that I have seen, and I have seen many. Rarely, outside the State of Florida, do I see PA's that do not cut frozens. Now, on to the issue of grossing techs. There are myriad reasons why I, and most of my peers, think it is not appropriate to utilize "grossing techs". For starters, and to be clear, the use of such techs serves one principal purpose to the pathologist's and institutions that employ them - to save money and increase their profits. They are not employed because they represent the clear choice for the utmost in patient care, and to suggest that is not just misleading, but completely false. Grossing small specimens is never just about transferring tissue from a container to a block. Many tend to try and downplay the importance of that task, and overlook things that could be problematic without certain training/skills. And, there are many grossing techs that do larger cases, from gallbladders all the way up to mastectomies and beyond - all with no didactic education, no proficiency testing and no rotations through various types of insitutions. I have never seen a study, but perhaps someone on here has, that points out the sharp increase in error rates found when a tech is used to gross, versus a trained pathologists' assistant. There is a drastic difference. It is distinct, and a study is really not needed to see that difference. Now, to be clear again, that is not to say that every tech that grosses does a bad job. No "vitriol" here. It is just a fact, and a troubling one at that. Imagine the difference in quality you would see if you had me doing all your stains! I am not trained as an HT. You could argue that I could be trained, but do you really want to open that can of worms? Do you want medicine to allow for that, and risk the HT profession? Probably not, and we do not either. Do you think I would be as good as you are, given all the real education you received when getting your HT training? I don't think I would be as good as you are at doing your job. As an example to illustrate, anyone that grosses should know how to answer these very basic questions. These might help shed some light on the issue: 1. What is the most common neoplasm of the gallbladder, what does it look like, and where is it found? Would you know it if you saw it? Is it benign, or malignant? 2. What is the reason that all appendices should have the margin submitted in the initial submission? 3. Would you know the difference between an esophageal bx and a bx from any other part of the GI tract simply by gross appearance? What would you do if you had 2 specimens, one esophagus and one duodenal and they were reversed in the specimen containers by the biopsy tech? Would you be able to pick up on that mistake? 4. How should you handle a skin punch for alopecia? 5. If sent a skin for frozen, and it was a pilomatrixoma, would you know it, and would you still freeze it, or ask the pathologist first to avoid doing an unnecessary frozen? 6. When are tangential margins more appropriate than perpendicular, and vice versa? You can ask me the same types of questions as they pertain to histology, and there is no chance I can answer them as correctly, and with as much clarity as a well trained HT. The bottom line is patient care and quality. I cannot provide the level of quality in histology as you can, and a grossing tech cannot provide that quality at the dissection bench. It just is not possible. The deal is simple - I will never downplay the value of your education and training by suggesting anyone can do it. All I ask is the same in return. Do not suggest that anyone can be trained on the job to do what it took me many years of college to learn, and perform that work at the same level. And I could not do your job nearly as well as you do. I am always impressed with histotechs - they have a great body of knowledge and do a very detailed, intricate and challenging job. For me to suggest, that given a few minutes here and there of training, that I can do your job as well as you would be very condescending. I don't think for one minute you could make me as good as you are by spending just a little bit of time with me. I know I would make mistakes, and mistakes may be considered part of the learning process, but do we really want to accept that in healthcare? Mistakes should happen in school, not with a real, live patients tissue. We should all be aiming to provide the highest level of care possible. I want my surgical specimen (biopsy or other) grossed by a PA, or MD and I want that specimen cut and stained by an ASCP certified HT. Lowering standards is a slippery slope, and one that should not be embarked upon in the world of medicine. I get crucified on this email server constantly. I have read and re-read the above. I see nothing in this to suggest condescension. For those of you that will see that no matter what, it is clearly personal for you, and for that I am sorry. Nothing here is meant to be offensive, just illustrative. On Wed, Apr 25, 2012 at 7:41 AM, Joanne Clark wrote: > > David, after reading your post I was not at all surprised to see that you > are a PA. I am assuming that explains your vitriol towards techs that > gross. Yes, CLIA does provide the educational requirements for high > complexity testing, but what on earth makes you think that a tech with the > proper CLIA qualifications can gross without proper training by a > pathologist? CAP requires that as well as extensive documentation of > training AND a list of the specimens approved by the Lab Director that a > 'non-pathologist' is allowed to gross. I'm sure you can tell that I am a > Histotech with an Associates Degree and I do the grossing in my lab. I can > assure you that I do a good job and if there is EVER any question regarding > how to gross in a specimen I will get a pathologist. To make it clear, > just because we tech's that gross do not have a masters as a pathologist > assistant, we care just as much about the patients we serve as a PA does. > Another point I would like to make is that very often we gross not by > choice but because it is what our pathologists demand of us and they > wouldn't put us there if we couldn't do the job. Believe me, when I say > that I do want to get my masters as a PA, but I haven't been able to find a > program that accommodates someone who is working full time and can not > afford to quit to go back to school. I am currently finishing up my > Bachelors, because I still want to pursue it. > > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > > ------------------------------ > > Message: 8 > Date: Mon, 23 Apr 2012 16:32:34 -0700 > From: Davide Costanzo > Subject: Re: [Histonet] Qualifications for grossing > To: Glen Dawson > Cc: histonet > Message-ID: > > > Content-Type: text/plain; charset=ISO-8859-1 > > Glen, > > Below are the requirements for high complexity testing, as outline by CLIA. > You can reference the CLIA '88 ruling, specifically look at Subpart M, > Section 493.1489 > > The requirements are weak, to say the least. I am not alone in the opinion > that just because CLIA allows it, it is not necessarily appropriate for the > minimum qualified person to be grossing certain specimens. Having someone > other than an M.D., or ASCP certified PA do anything larger than a skin > shave is not good medicine. But, in answer to your question - yes, the > government allows inadequately trained personnel to perform high complexity > testing. > > > Sec. 493.1489 Standard; Testing personnel qualifications. > > > > Each individual performing high complexity testing must-- > > (a) Possess a current license issued by the State in which the > > laboratory is located, if such licensing is required; and > > (b) Meet one of the following requirements: > > (1) Be a doctor of medicine, doctor of osteopathy, or doctor of > > podiatric medicine licensed to practice medicine, osteopathy, or > > podiatry in the State in which the laboratory is located or have > earned > > a doctoral, master's or bachelor's degree in a chemical, physical, > > biological or clinical laboratory science, or medical technology from > an > > accredited institution; > > (2)(i) Have earned an associate degree in a laboratory science, or > > medical laboratory technology from an accredited institution or-- > > (ii) Have education and training equivalent to that specified in > > paragraph (b)(2)(i) of this section that includes-- > > (A) At least 60 semester hours, or equivalent, from an accredited > > institution that, at a minimum, include either-- > > (1) 24 semester hours of medical laboratory technology courses; or > > (2) 24 semester hours of science courses that include-- > > (i) Six semester hours of chemistry; > > (ii) Six semester hours of biology; and > > (iii) Twelve semester hours of chemistry, biology, or medical > > laboratory technology in any combination; and > > (B) Have laboratory training that includes either of the following: > > (1) Completion of a clinical laboratory training program approved or > > accredited by the ABHES, the CAHEA, or other organization approved by > > HHS. (This training may be included in the 60 semester hours listed in > > paragraph (b)(2)(ii)(A) of this section.) > > (2) At least 3 months documented laboratory training in each > > specialty in which the individual performs high complexity testing. > > (3) Have previously qualified or could have qualified as a > technologist under Sec. 493.1491 on or before February 28, 1992 > > On Mon, Apr 23, 2012 at 1:19 PM, Glen Dawson > wrote: > > > > > All, > > > > Can a histotech perform GROSSING if he/she has an associate's degree > > in Histotechnology from an accredited institution (Argosy in MN)? > > > > Any help would be appreciated. > > > > Thank-you, > > > > Glen Dawson BS, HT(ASCP) & QIHC > > Histology Technical Specialist > > Mercy Health System > > Janesville, WI > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From rjbuesa <@t> yahoo.com Wed Apr 25 11:55:55 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 25 11:56:04 2012 Subject: [Histonet] RE: Qualifications for grossing In-Reply-To: Message-ID: <1335372955.97357.YahooMailClassic@web162102.mail.bf1.yahoo.com> For what little it may mean, I wholeheartly agree with Davice Costanzo e-mail. I completely agree with him. In the same way the economic situation we are now was caused by greed, that is the motor guiding those who, to just save money, let a histotech (ologist) to do grossing. Grossing,, especially large complex specimens, is the fundamental initial task in any complex diagnosis. The PA is the one who SELECTS what is going to be processed and used for diagnosis. IF some part of the specimen is not submitted as the result of ignorance caused by poor training, the worst thing could happen, namely, a FALSE NEGATIVE --- On Wed, 4/25/12, Davide Costanzo wrote: From: Davide Costanzo Subject: Re: [Histonet] RE: Qualifications for grossing To: "Joanne Clark" Cc: "histonet@lists.utsouthwestern.edu" Date: Wednesday, April 25, 2012, 12:34 PM Joanna, I wanted to take an opportunity to explain my, and most of my colleagues, feelings about CLIA '88 with respect to grossing standards. But I want to start by stating that this goes both ways, I also do not feel it is appropriate for an ASCP certified PA to be performing Immunohistochemistry, or other stains in the lab. Both histotechnicians (ologists) and PA's have a very clear role in the pathology laboratory. Both have very different training programs. Both HT's and PA's should be protected by law, and rules/regulations for each should be clear. One is not better than the other, and I certainly hope you do not think I have an opinion different from that. Both are highly qualified individuals in their area of expertise. In many states, and I will use Florida as an example because that is what I am familiar with, there are clear definitions in the law as to whom can perform what tasks. In the State of Florida, a PA (regardless of training level) is not to perform frozen sections. That State only allows Pathologists and HT's to cut a frozen. This is the result of much effort put in to changing those rules by the HT's in Florida. Clearly they saw PA's as a threat to their job, and took action. Not a problem, I am happy to let them do the frozen sections. What was it about cutting a frozen section that the HT's thought a PA could not handle? I do not know, but nonetheless they reacted. Certainly PA's are heavily trained in how to cut a frozen section, and it is generally considered our responsibility in most places in the US that I have seen, and I have seen many. Rarely, outside the State of Florida, do I see PA's that do not cut frozens. Now, on to the issue of grossing techs. There are myriad reasons why I, and most of my peers, think it is not appropriate to utilize "grossing techs". For starters, and to be clear, the use of such techs serves one principal purpose to the pathologist's and institutions that employ them - to save money and increase their profits. They are not employed because they represent the clear choice for the utmost in patient care, and to suggest that is not just misleading, but completely false. Grossing small specimens is never just about transferring tissue from a container to a block. Many tend to try and downplay the importance of that task, and overlook things that could be problematic without certain training/skills. And, there are many grossing techs that do larger cases, from gallbladders all the way up to mastectomies and beyond - all with no didactic education, no proficiency testing and no rotations through various types of insitutions. I have never seen a study, but perhaps someone on here has, that points out the sharp increase in error rates found when a tech is used to gross, versus a trained pathologists' assistant. There is a drastic difference. It is distinct, and a study is really not needed to see that difference. Now, to be clear again, that is not to say that every tech that grosses does a bad job. No "vitriol" here. It is just a fact, and a troubling one at that. Imagine the difference in quality you would see if you had me doing all your stains! I am not trained as an HT. You could argue that I could be trained, but do you really want to open that can of worms? Do you want medicine to allow for that, and risk the HT profession? Probably not, and we do not either.? Do you think I would be as good as you are, given all the real education you received when getting your HT training? I don't think I would be as good as you are at doing your job. As an example to illustrate, anyone that grosses should know how to answer these very basic questions. These might help shed some light on the issue: ???1. What is the most common neoplasm of the gallbladder, what does it ???look like, and where is it found? Would you know it if you saw it? Is it ???benign, or malignant? ???2. What is the reason that all appendices should have the margin ???submitted in the initial submission? ???3. Would you know the difference between an esophageal bx and a bx from ???any other part of the GI tract simply by gross appearance? What would you ???do if you had 2 specimens, one esophagus and one duodenal and they were ???reversed in the specimen containers by the biopsy tech? Would you be able ???to pick up on that mistake? ???4. How should you handle a skin punch for alopecia? ???5. If sent a skin for frozen, and it was a pilomatrixoma, would you know ???it, and would you still freeze it, or ask the pathologist first to avoid ???doing an unnecessary frozen? ???6. When are tangential margins more appropriate than perpendicular, and ???vice versa? You can ask me the same types of questions as they pertain to histology, and there is no chance I can answer them as correctly, and with as much clarity as a well trained HT. The bottom line is patient care and quality. I cannot provide the level of quality in histology as you can, and a grossing tech cannot provide that quality at the dissection bench. It just is not possible. The deal is simple - I will never downplay the value of your education and training by suggesting anyone can do it. All I ask is the same in return. Do not suggest that anyone can be trained on the job to do what it took me many years of college to learn, and perform that work at the same level. And I could not do your job nearly as well as you do. I am always impressed with histotechs - they have a great body of knowledge and do a very detailed, intricate and challenging job. For me to suggest, that given a few minutes here and there of training, that I can do your job as well as you would be very condescending. I don't think for one minute you could make me as good as you are by spending just a little bit of time with me. I know I would make mistakes, and mistakes may be considered part of the learning process, but do we really want to accept that in healthcare? Mistakes should happen in school, not with a real, live patients tissue. We should all be aiming to provide the highest level of care possible. I want my surgical specimen (biopsy or other) grossed by a PA, or MD and I want that specimen cut and stained by an ASCP certified HT. Lowering standards is a slippery slope, and one that should not be embarked upon in the world of medicine. I get crucified on this email server constantly. I have read and re-read the above. I see nothing in this to suggest condescension. For those of you that will see that no matter what, it is clearly personal for you, and for that I am sorry. Nothing here is meant to be offensive, just illustrative. On Wed, Apr 25, 2012 at 7:41 AM, Joanne Clark wrote: > > David, after reading your post I was not at all surprised to see that you > are a PA.? I am assuming that explains your vitriol towards techs that > gross.? Yes, CLIA does provide the educational requirements for high > complexity testing, but what on earth makes you think that a tech with the > proper CLIA qualifications can gross without proper training by a > pathologist?? CAP requires that as well as extensive documentation of > training AND a list of the specimens approved by the Lab Director that a > 'non-pathologist' is allowed to gross.? I'm sure you can tell that I am a > Histotech with an Associates Degree and I do the grossing in my lab.? I can > assure you that I do a good job and if there is EVER any question regarding > how to gross in a specimen I will get a pathologist.? To make it clear, > just because we tech's that gross do not have a masters as a pathologist > assistant, we care just as much about the patients we serve as a PA does. >? Another point I would like to make is that very often we gross not by > choice but because it is what our pathologists demand of us and they > wouldn't put us there if we couldn't do the job. Believe me, when I say > that I do want to get my masters as a PA, but I haven't been able to find a > program that accommodates someone who is working full time and can not > afford to quit to go back to school.? I am currently finishing up my > Bachelors, because I still want to pursue it. > > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > > ------------------------------ > > Message: 8 > Date: Mon, 23 Apr 2012 16:32:34 -0700 > From: Davide Costanzo > Subject: Re: [Histonet] Qualifications for grossing > To: Glen Dawson > Cc: histonet > Message-ID: >? ? ? ? > > Content-Type: text/plain; charset=ISO-8859-1 > > Glen, > > Below are the requirements for high complexity testing, as outline by CLIA. > You can reference the CLIA '88 ruling, specifically look at Subpart M, > Section 493.1489 > > The requirements are weak, to say the least. I am not alone in the opinion > that just because CLIA allows it, it is not necessarily appropriate for the > minimum qualified person to be grossing certain specimens. Having someone > other than an M.D., or ASCP certified PA do anything larger than a skin > shave is not good medicine. But, in answer to your question - yes, the > government allows inadequately trained personnel to perform high complexity > testing. > > > Sec. 493.1489? Standard; Testing personnel qualifications. > > > >? ? Each individual performing high complexity testing must-- > >? ? (a) Possess a current license issued by the State in which the > >? ? ? laboratory is located, if such licensing is required; and > >? ? (b) Meet one of the following requirements: > >? ? (1) Be a doctor of medicine, doctor of osteopathy, or doctor of > >? ? ? podiatric medicine licensed to practice medicine, osteopathy, or > >? ? ? podiatry in the State in which the laboratory is located or have > earned > >? ? ? a doctoral, master's or bachelor's degree in a chemical, physical, > >? ? ? biological or clinical laboratory science, or medical technology from > an > >? ? ? accredited institution; > >? ? (2)(i) Have earned an associate degree in a laboratory science, or > >? ? ? medical laboratory technology from an accredited institution or-- > >? ? (ii) Have education and training equivalent to that specified in > >? ? ? paragraph (b)(2)(i) of this section that includes-- > >? ? (A) At least 60 semester hours, or equivalent, from an accredited > >? ? ? institution that, at a minimum, include either-- > >? ? (1) 24 semester hours of medical laboratory technology courses; or > >? ? (2) 24 semester hours of science courses that include-- > >? ? (i) Six semester hours of chemistry; > >? ? (ii) Six semester hours of biology; and > >? ? (iii) Twelve semester hours of chemistry, biology, or medical > >? ? ? laboratory technology in any combination; and > >? ???(B) Have laboratory training that includes either of the following: > >? ? (1) Completion of a clinical laboratory training program approved or > >? ? ? accredited by the ABHES, the CAHEA, or other organization approved by > >? ? ? HHS. (This training may be included in the 60 semester hours listed in > >? ? ? paragraph (b)(2)(ii)(A) of this section.) > >? ? (2) At least 3 months documented laboratory training in each > >? ? ? specialty in which the individual performs high complexity testing. > >? ? (3) Have previously qualified or could have qualified as a >? ? ? technologist under Sec. 493.1491 on or before February 28, 1992 > > On Mon, Apr 23, 2012 at 1:19 PM, Glen Dawson > wrote: > > > > > All, > > > > Can a histotech perform GROSSING if he/she has an associate's degree > > in Histotechnology from an accredited institution (Argosy in MN)? > > > > Any help would be appreciated. > > > > Thank-you, > > > > Glen Dawson BS, HT(ASCP) & QIHC > > Histology Technical Specialist > > Mercy Health System > > Janesville, WI > >? _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdboydhisto <@t> yahoo.com Wed Apr 25 12:00:40 2012 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Wed Apr 25 12:00:46 2012 Subject: [Histonet] cloud based LIS Message-ID: <1335373240.85758.YahooMailNeo@web125802.mail.ne1.yahoo.com> ?Is there anyone out there in Histoland who is using a cloud based LIS?? Kelly? From Courtney.Pierce <@t> quintiles.com Wed Apr 25 12:02:16 2012 From: Courtney.Pierce <@t> quintiles.com (Courtney Pierce) Date: Wed Apr 25 12:02:26 2012 Subject: [Histonet] Problems with pAKT. Message-ID: Has anyone had cross reacting problems with pAKT. I have used both Cell Signaling #3787 and #4606. Can anyone help me with this. Thanks Courtney Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 610 Oakmont Lane Westmont, IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ From CIngles <@t> uwhealth.org Wed Apr 25 12:41:29 2012 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Apr 25 12:43:50 2012 Subject: [Histonet] RE: Qualifications for grossing References: <1335372955.97357.YahooMailClassic@web162102.mail.bf1.yahoo.com> Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A834@UWHC-MAIL2.uwhis.hosp.wisc.edu> You can reprocess, recut, and restain, but never re-gross. I for one also gross, but only skin. And yes, I DO know how to gross an alopecia specimen. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Wed 4/25/2012 11:55 AM To: Joanne Clark; Davide Costanzo Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Qualifications for grossing For what little it may mean, I wholeheartly agree with Davice Costanzo e-mail. I completely agree with him. In the same way the economic situation we are now was caused by greed, that is the motor guiding those who, to just save money, let a histotech (ologist) to do grossing. Grossing,, especially large complex specimens, is the fundamental initial task in any complex diagnosis. The PA is the one who SELECTS what is going to be processed and used for diagnosis. IF some part of the specimen is not submitted as the result of ignorance caused by poor training, the worst thing could happen, namely, a FALSE NEGATIVE --- On Wed, 4/25/12, Davide Costanzo wrote: From pathlocums <@t> gmail.com Wed Apr 25 13:34:40 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Wed Apr 25 13:34:43 2012 Subject: [Histonet] RE: Qualifications for grossing Message-ID: <3709333745208014141@unknownmsgid> Nothing is black and white. Perhaps exceptions are in order where the tech only does one specimen type. Perhaps. But not where techs do a lot more. There are gross techs out there doing colons, mastectomies etc. This is where big trouble brews. This is where people are not adequately trained, by NAACLS standards. CLIA '88 is the problem, not the tech. CLIA is over 20 years old, and at that time PA's were fairly new on the scene and in short supply. That is not the case today. It is time to raise the bar and improve patient care. There are no valid excuses. Today, this is no more than financial greed that accounts for misuse of personnel. Sent from my Windows Phone From: Ingles Claire Sent: 4/25/2012 10:43 AM To: Rene J Buesa; Joanne Clark; Davide Costanzo Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Qualifications for grossing You can reprocess, recut, and restain, but never re-gross. I for one also gross, but only skin. And yes, I DO know how to gross an alopecia specimen. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Wed 4/25/2012 11:55 AM To: Joanne Clark; Davide Costanzo Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Qualifications for grossing For what little it may mean, I wholeheartly agree with Davice Costanzo e-mail. I completely agree with him. In the same way the economic situation we are now was caused by greed, that is the motor guiding those who, to just save money, let a histotech (ologist) to do grossing. Grossing,, especially large complex specimens, is the fundamental initial task in any complex diagnosis. The PA is the one who SELECTS what is going to be processed and used for diagnosis. IF some part of the specimen is not submitted as the result of ignorance caused by poor training, the worst thing could happen, namely, a FALSE NEGATIVE --- On Wed, 4/25/12, Davide Costanzo wrote: From nicole <@t> dlcjax.com Wed Apr 25 14:08:19 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed Apr 25 14:08:30 2012 Subject: [Histonet] RE: Qualifications for grossing In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3784CB7F4B5@S10MAILD001N1.SH10.lan> References: <20120424170509.F2E758B70FC@mx10.myoutlookonline.com> <0494A7D4E8CC254EA2FB81464982E3784CB7F4B5@S10MAILD001N1.SH10.lan> Message-ID: <2510.208.62.167.196.1335380899.squirrel@webmail.realpages.com> Yupp he strikes again. Joanne, I strongly agree with your perspective. Many Techs do not have formal expensive educations and have sat on the bench for many years and eventually became grandfathered in. Those techs are the life blood of pathology. It has only been in recent years that licensure has become a larger part of health care requiring personal to obtain certification to "hopefully" increase patient care. But, this argument is becoming a thing of the past, because CLIA, CAP, JOCA have set standards that personal must meet regardless of the extensive OJT. I am qualified to gross based on these accrediators standards. It is others opinions that think these "standards" are weak. If the argument is greed, than people should understand that employee payroll is the highest cost within a laboratory so to help cut cost to our bankrupt health care system, why not pay a Histologist who is clearly qualified to do a job they have been doing since the beginning of pathology. The pathologists? assistant profession began in 1969 with a pilot training program at the Veterans Administration Medical Center (VAMC) in Durham, North Carolina. In 1856 William Perkin discovered the dye mauve that was used in the early 1860s by F W B Benke of Marlbery. Joseph Janavier Woodward, a surgeon in the US Army, used fuchsine and aniline blue to stain human intestines. Paul Ehrlich realized that the chemical dyes obtained from coal tar did not simply color cells but combined with the chemical elements within them to form new substances. The Swiss chemist Friedrich Miescher, in 1869 used aniline dyes to examine the cell nucleus. In 1875 Carl Weigart, Ehrlich's cousin, demonstrated the fuchsine derivative methyl violet stained bacteria as opposed to tissue. The first histologist, Marcello Malpighi (1628-1694), an Italian anatomist, is in fact considered the true ?Father of Histology?. 1819, A. Mayer created the term Histology. In the sequence of the previous word tissue, made use of two classical Greek root words (histos = tissue and logos = study So, my point is I do believe Histo's have been involved since the very beginning. We as a profession have a combined experience well beyond that of any formal education. Last thought, and I quote, "I know I would make mistakes, and mistakes may be considered part of the learning process, but do we really want to accept that in health care? Mistakes should happen in school, not with a real, live patients tissue." This statement clearly conflicts with all aspect of becoming a medical professional. Our state/government funded hospitals employ thousands of residents each year who treat thousands of indigent and paying patients. This is their school. Histologist do interships within hospitals to get training. This is their school to. Nurses, MLT's, MA's, everyone in health care learns the actual (beyond books)trade from watching and working with skilled persons who have many years of experience. They would not place a student with a person who has a degree but no experience. My education qualifies me to be trained by anyone in my field of pathology, and I should be used where my skills will best serve my department and increase patient care. Each one of us serves a purpose and is valuable, no matter what job we perform with what amount of education. The law is weeding out those who are no longer qualified to work in out field, they set the standard. Let's let them make the decisions on who is qualified to do what and stick together to ensure its fair to each one of us. Can't we all just get along...hehehehehe Nicole Tatum HT, ASCP From Matthew.Smith <@t> moffitt.org Wed Apr 25 14:18:09 2012 From: Matthew.Smith <@t> moffitt.org (Smith, Matthew A.) Date: Wed Apr 25 14:18:17 2012 Subject: [Histonet] size of cut/transfer method affect staining? Message-ID: <73768D158B02FF4B8DB5DAE0A7312EF6D99C@EXMB03.hlm.ad.moffitt.usf.edu> Hi, I am attempting to do dual staining with 2 antibodies. One works fine but I am having trouble getting consistent staining with the second. I recently noticed previous user was requesting 3uM microtomy sections on tape, then going TPC, xylenes/etoh, AR,etc. I have been using 4um floated sections and can just not see the same staining using same everything else. Does anyone have thoughts about this? I was thinking perhaps the TPC/tape leaves the cells more permeable or affects AR efficiency. I am currently going to test side-by-side...just trying to get a general opinion on the matter as immunohistochemistry is not my area of proficiency. Matthew A. Smith, PhD MSPH Postdoctoral Fellow, Clinical Investigations Laboratory of Eric Haura, MD tel: 813-745-6193 | email: Matthew.Smith@moffitt.org P Please consider the environment before printing this email. This transmission may be confidential or protected from disclosure and is only for review and use by the intended recipient. Access by anyone else is unauthorized. Any unauthorized reader is hereby notified that any review, use, dissemination, disclosure or copying of this information, or any act or omission taken in reliance on it, is prohibited and may be unlawful. If you received this transmission in error, please notify the sender immediately. Thank you. From HornHV <@t> archildrens.org Wed Apr 25 14:18:53 2012 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Apr 25 14:18:58 2012 Subject: [Histonet] RE: Qualifications for grossing In-Reply-To: References: <20120424170509.F2E758B70FC@mx10.myoutlookonline.com> <0494A7D4E8CC254EA2FB81464982E3784CB7F4B5@S10MAILD001N1.SH10.lan> Message-ID: <25A4DE08332B19499904459F00AAACB719B9F3E67D@EVS1.archildrens.org> Well said. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo Sent: Wednesday, April 25, 2012 11:34 AM To: Joanne Clark Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Qualifications for grossing Joanna, I wanted to take an opportunity to explain my, and most of my colleagues, feelings about CLIA '88 with respect to grossing standards. But I want to start by stating that this goes both ways, I also do not feel it is appropriate for an ASCP certified PA to be performing Immunohistochemistry, or other stains in the lab. Both histotechnicians (ologists) and PA's have a very clear role in the pathology laboratory. Both have very different training programs. Both HT's and PA's should be protected by law, and rules/regulations for each should be clear. One is not better than the other, and I certainly hope you do not think I have an opinion different from that. Both are highly qualified individuals in their area of expertise. In many states, and I will use Florida as an example because that is what I am familiar with, there are clear definitions in the law as to whom can perform what tasks. In the State of Florida, a PA (regardless of training level) is not to perform frozen sections. That State only allows Pathologists and HT's to cut a frozen. This is the result of much effort put in to changing those rules by the HT's in Florida. Clearly they saw PA's as a threat to their job, and took action. Not a problem, I am happy to let them do the frozen sections. What was it about cutting a frozen section that the HT's thought a PA could not handle? I do not know, but nonetheless they reacted. Certainly PA's are heavily trained in how to cut a frozen section, and it is generally considered our responsibility in most places in the US that I have seen, and I have seen many. Rarely, outside the State of Florida, do I see PA's that do not cut frozens. Now, on to the issue of grossing techs. There are myriad reasons why I, and most of my peers, think it is not appropriate to utilize "grossing techs". For starters, and to be clear, the use of such techs serves one principal purpose to the pathologist's and institutions that employ them - to save money and increase their profits. They are not employed because they represent the clear choice for the utmost in patient care, and to suggest that is not just misleading, but completely false. Grossing small specimens is never just about transferring tissue from a container to a block. Many tend to try and downplay the importance of that task, and overlook things that could be problematic without certain training/skills. And, there are many grossing techs that do larger cases, from gallbladders all the way up to mastectomies and beyond - all with no didactic education, no proficiency testing and no rotations through various types of insitutions. I have never seen a study, but perhaps someone on here has, that points out the sharp increase in error rates found when a tech is used to gross, versus a trained pathologists' assistant. There is a drastic difference. It is distinct, and a study is really not needed to see that difference. Now, to be clear again, that is not to say that every tech that grosses does a bad job. No "vitriol" here. It is just a fact, and a troubling one at that. Imagine the difference in quality you would see if you had me doing all your stains! I am not trained as an HT. You could argue that I could be trained, but do you really want to open that can of worms? Do you want medicine to allow for that, and risk the HT profession? Probably not, and we do not either. Do you think I would be as good as you are, given all the real education you received when getting your HT training? I don't think I would be as good as you are at doing your job. As an example to illustrate, anyone that grosses should know how to answer these very basic questions. These might help shed some light on the issue: 1. What is the most common neoplasm of the gallbladder, what does it look like, and where is it found? Would you know it if you saw it? Is it benign, or malignant? 2. What is the reason that all appendices should have the margin submitted in the initial submission? 3. Would you know the difference between an esophageal bx and a bx from any other part of the GI tract simply by gross appearance? What would you do if you had 2 specimens, one esophagus and one duodenal and they were reversed in the specimen containers by the biopsy tech? Would you be able to pick up on that mistake? 4. How should you handle a skin punch for alopecia? 5. If sent a skin for frozen, and it was a pilomatrixoma, would you know it, and would you still freeze it, or ask the pathologist first to avoid doing an unnecessary frozen? 6. When are tangential margins more appropriate than perpendicular, and vice versa? You can ask me the same types of questions as they pertain to histology, and there is no chance I can answer them as correctly, and with as much clarity as a well trained HT. The bottom line is patient care and quality. I cannot provide the level of quality in histology as you can, and a grossing tech cannot provide that quality at the dissection bench. It just is not possible. The deal is simple - I will never downplay the value of your education and training by suggesting anyone can do it. All I ask is the same in return. Do not suggest that anyone can be trained on the job to do what it took me many years of college to learn, and perform that work at the same level. And I could not do your job nearly as well as you do. I am always impressed with histotechs - they have a great body of knowledge and do a very detailed, intricate and challenging job. For me to suggest, that given a few minutes here and there of training, that I can do your job as well as you would be very condescending. I don't think for one minute you could make me as good as you are by spending just a little bit of time with me. I know I would make mistakes, and mistakes may be considered part of the learning process, but do we really want to accept that in healthcare? Mistakes should happen in school, not with a real, live patients tissue. We should all be aiming to provide the highest level of care possible. I want my surgical specimen (biopsy or other) grossed by a PA, or MD and I want that specimen cut and stained by an ASCP certified HT. Lowering standards is a slippery slope, and one that should not be embarked upon in the world of medicine. I get crucified on this email server constantly. I have read and re-read the above. I see nothing in this to suggest condescension. For those of you that will see that no matter what, it is clearly personal for you, and for that I am sorry. Nothing here is meant to be offensive, just illustrative. On Wed, Apr 25, 2012 at 7:41 AM, Joanne Clark wrote: > > David, after reading your post I was not at all surprised to see that > you are a PA. I am assuming that explains your vitriol towards techs > that gross. Yes, CLIA does provide the educational requirements for > high complexity testing, but what on earth makes you think that a tech > with the proper CLIA qualifications can gross without proper training > by a pathologist? CAP requires that as well as extensive > documentation of training AND a list of the specimens approved by the > Lab Director that a 'non-pathologist' is allowed to gross. I'm sure > you can tell that I am a Histotech with an Associates Degree and I do > the grossing in my lab. I can assure you that I do a good job and if > there is EVER any question regarding how to gross in a specimen I will > get a pathologist. To make it clear, just because we tech's that > gross do not have a masters as a pathologist assistant, we care just as much about the patients we serve as a PA does. > Another point I would like to make is that very often we gross not by > choice but because it is what our pathologists demand of us and they > wouldn't put us there if we couldn't do the job. Believe me, when I > say that I do want to get my masters as a PA, but I haven't been able > to find a program that accommodates someone who is working full time > and can not afford to quit to go back to school. I am currently > finishing up my Bachelors, because I still want to pursue it. > > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > > ------------------------------ > > Message: 8 > Date: Mon, 23 Apr 2012 16:32:34 -0700 > From: Davide Costanzo > Subject: Re: [Histonet] Qualifications for grossing > To: Glen Dawson > Cc: histonet > Message-ID: > > > > Content-Type: text/plain; charset=ISO-8859-1 > > Glen, > > Below are the requirements for high complexity testing, as outline by CLIA. > You can reference the CLIA '88 ruling, specifically look at Subpart M, > Section 493.1489 > > The requirements are weak, to say the least. I am not alone in the > opinion that just because CLIA allows it, it is not necessarily > appropriate for the minimum qualified person to be grossing certain > specimens. Having someone other than an M.D., or ASCP certified PA do > anything larger than a skin shave is not good medicine. But, in answer > to your question - yes, the government allows inadequately trained > personnel to perform high complexity testing. > > > Sec. 493.1489 Standard; Testing personnel qualifications. > > > > Each individual performing high complexity testing must-- > > (a) Possess a current license issued by the State in which the > > laboratory is located, if such licensing is required; and > > (b) Meet one of the following requirements: > > (1) Be a doctor of medicine, doctor of osteopathy, or doctor of > > podiatric medicine licensed to practice medicine, osteopathy, or > > podiatry in the State in which the laboratory is located or have > earned > > a doctoral, master's or bachelor's degree in a chemical, > physical, > > biological or clinical laboratory science, or medical technology > from an > > accredited institution; > > (2)(i) Have earned an associate degree in a laboratory science, or > > medical laboratory technology from an accredited institution or-- > > (ii) Have education and training equivalent to that specified in > > paragraph (b)(2)(i) of this section that includes-- > > (A) At least 60 semester hours, or equivalent, from an accredited > > institution that, at a minimum, include either-- > > (1) 24 semester hours of medical laboratory technology courses; or > > (2) 24 semester hours of science courses that include-- > > (i) Six semester hours of chemistry; > > (ii) Six semester hours of biology; and > > (iii) Twelve semester hours of chemistry, biology, or medical > > laboratory technology in any combination; and > > (B) Have laboratory training that includes either of the following: > > (1) Completion of a clinical laboratory training program approved > or > > accredited by the ABHES, the CAHEA, or other organization > approved by > > HHS. (This training may be included in the 60 semester hours > listed in > > paragraph (b)(2)(ii)(A) of this section.) > > (2) At least 3 months documented laboratory training in each > > specialty in which the individual performs high complexity testing. > > (3) Have previously qualified or could have qualified as a > technologist under Sec. 493.1491 on or before February 28, 1992 > > On Mon, Apr 23, 2012 at 1:19 PM, Glen Dawson > wrote: > > > > > All, > > > > Can a histotech perform GROSSING if he/she has an associate's degree > > in Histotechnology from an accredited institution (Argosy in MN)? > > > > Any help would be appreciated. > > > > Thank-you, > > > > Glen Dawson BS, HT(ASCP) & QIHC > > Histology Technical Specialist > > Mercy Health System > > Janesville, WI > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From pathlocums <@t> gmail.com Wed Apr 25 14:24:22 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Wed Apr 25 14:24:26 2012 Subject: [Histonet] RE: Qualifications for grossing Message-ID: <1934443837708879721@unknownmsgid> Thank you Hazel. Sent from my Windows Phone From: Horn, Hazel V Sent: 4/25/2012 12:18 PM To: Davide Costanzo; Joanne Clark Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Qualifications for grossing Well said. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo Sent: Wednesday, April 25, 2012 11:34 AM To: Joanne Clark Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Qualifications for grossing Joanna, I wanted to take an opportunity to explain my, and most of my colleagues, feelings about CLIA '88 with respect to grossing standards. But I want to start by stating that this goes both ways, I also do not feel it is appropriate for an ASCP certified PA to be performing Immunohistochemistry, or other stains in the lab. Both histotechnicians (ologists) and PA's have a very clear role in the pathology laboratory. Both have very different training programs. Both HT's and PA's should be protected by law, and rules/regulations for each should be clear. One is not better than the other, and I certainly hope you do not think I have an opinion different from that. Both are highly qualified individuals in their area of expertise. In many states, and I will use Florida as an example because that is what I am familiar with, there are clear definitions in the law as to whom can perform what tasks. In the State of Florida, a PA (regardless of training level) is not to perform frozen sections. That State only allows Pathologists and HT's to cut a frozen. This is the result of much effort put in to changing those rules by the HT's in Florida. Clearly they saw PA's as a threat to their job, and took action. Not a problem, I am happy to let them do the frozen sections. What was it about cutting a frozen section that the HT's thought a PA could not handle? I do not know, but nonetheless they reacted. Certainly PA's are heavily trained in how to cut a frozen section, and it is generally considered our responsibility in most places in the US that I have seen, and I have seen many. Rarely, outside the State of Florida, do I see PA's that do not cut frozens. Now, on to the issue of grossing techs. There are myriad reasons why I, and most of my peers, think it is not appropriate to utilize "grossing techs". For starters, and to be clear, the use of such techs serves one principal purpose to the pathologist's and institutions that employ them - to save money and increase their profits. They are not employed because they represent the clear choice for the utmost in patient care, and to suggest that is not just misleading, but completely false. Grossing small specimens is never just about transferring tissue from a container to a block. Many tend to try and downplay the importance of that task, and overlook things that could be problematic without certain training/skills. And, there are many grossing techs that do larger cases, from gallbladders all the way up to mastectomies and beyond - all with no didactic education, no proficiency testing and no rotations through various types of insitutions. I have never seen a study, but perhaps someone on here has, that points out the sharp increase in error rates found when a tech is used to gross, versus a trained pathologists' assistant. There is a drastic difference. It is distinct, and a study is really not needed to see that difference. Now, to be clear again, that is not to say that every tech that grosses does a bad job. No "vitriol" here. It is just a fact, and a troubling one at that. Imagine the difference in quality you would see if you had me doing all your stains! I am not trained as an HT. You could argue that I could be trained, but do you really want to open that can of worms? Do you want medicine to allow for that, and risk the HT profession? Probably not, and we do not either. Do you think I would be as good as you are, given all the real education you received when getting your HT training? I don't think I would be as good as you are at doing your job. As an example to illustrate, anyone that grosses should know how to answer these very basic questions. These might help shed some light on the issue: 1. What is the most common neoplasm of the gallbladder, what does it look like, and where is it found? Would you know it if you saw it? Is it benign, or malignant? 2. What is the reason that all appendices should have the margin submitted in the initial submission? 3. Would you know the difference between an esophageal bx and a bx from any other part of the GI tract simply by gross appearance? What would you do if you had 2 specimens, one esophagus and one duodenal and they were reversed in the specimen containers by the biopsy tech? Would you be able to pick up on that mistake? 4. How should you handle a skin punch for alopecia? 5. If sent a skin for frozen, and it was a pilomatrixoma, would you know it, and would you still freeze it, or ask the pathologist first to avoid doing an unnecessary frozen? 6. When are tangential margins more appropriate than perpendicular, and vice versa? You can ask me the same types of questions as they pertain to histology, and there is no chance I can answer them as correctly, and with as much clarity as a well trained HT. The bottom line is patient care and quality. I cannot provide the level of quality in histology as you can, and a grossing tech cannot provide that quality at the dissection bench. It just is not possible. The deal is simple - I will never downplay the value of your education and training by suggesting anyone can do it. All I ask is the same in return. Do not suggest that anyone can be trained on the job to do what it took me many years of college to learn, and perform that work at the same level. And I could not do your job nearly as well as you do. I am always impressed with histotechs - they have a great body of knowledge and do a very detailed, intricate and challenging job. For me to suggest, that given a few minutes here and there of training, that I can do your job as well as you would be very condescending. I don't think for one minute you could make me as good as you are by spending just a little bit of time with me. I know I would make mistakes, and mistakes may be considered part of the learning process, but do we really want to accept that in healthcare? Mistakes should happen in school, not with a real, live patients tissue. We should all be aiming to provide the highest level of care possible. I want my surgical specimen (biopsy or other) grossed by a PA, or MD and I want that specimen cut and stained by an ASCP certified HT. Lowering standards is a slippery slope, and one that should not be embarked upon in the world of medicine. I get crucified on this email server constantly. I have read and re-read the above. I see nothing in this to suggest condescension. For those of you that will see that no matter what, it is clearly personal for you, and for that I am sorry. Nothing here is meant to be offensive, just illustrative. On Wed, Apr 25, 2012 at 7:41 AM, Joanne Clark wrote: > > David, after reading your post I was not at all surprised to see that > you are a PA. I am assuming that explains your vitriol towards techs > that gross. Yes, CLIA does provide the educational requirements for > high complexity testing, but what on earth makes you think that a tech > with the proper CLIA qualifications can gross without proper training > by a pathologist? CAP requires that as well as extensive > documentation of training AND a list of the specimens approved by the > Lab Director that a 'non-pathologist' is allowed to gross. I'm sure > you can tell that I am a Histotech with an Associates Degree and I do > the grossing in my lab. I can assure you that I do a good job and if > there is EVER any question regarding how to gross in a specimen I will > get a pathologist. To make it clear, just because we tech's that > gross do not have a masters as a pathologist assistant, we care just as much about the patients we serve as a PA does. > Another point I would like to make is that very often we gross not by > choice but because it is what our pathologists demand of us and they > wouldn't put us there if we couldn't do the job. Believe me, when I > say that I do want to get my masters as a PA, but I haven't been able > to find a program that accommodates someone who is working full time > and can not afford to quit to go back to school. I am currently > finishing up my Bachelors, because I still want to pursue it. > > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > > ------------------------------ > > Message: 8 > Date: Mon, 23 Apr 2012 16:32:34 -0700 > From: Davide Costanzo > Subject: Re: [Histonet] Qualifications for grossing > To: Glen Dawson > Cc: histonet > Message-ID: > > > > Content-Type: text/plain; charset=ISO-8859-1 > > Glen, > > Below are the requirements for high complexity testing, as outline by CLIA. > You can reference the CLIA '88 ruling, specifically look at Subpart M, > Section 493.1489 > > The requirements are weak, to say the least. I am not alone in the > opinion that just because CLIA allows it, it is not necessarily > appropriate for the minimum qualified person to be grossing certain > specimens. Having someone other than an M.D., or ASCP certified PA do > anything larger than a skin shave is not good medicine. But, in answer > to your question - yes, the government allows inadequately trained > personnel to perform high complexity testing. > > > Sec. 493.1489 Standard; Testing personnel qualifications. > > > > Each individual performing high complexity testing must-- > > (a) Possess a current license issued by the State in which the > > laboratory is located, if such licensing is required; and > > (b) Meet one of the following requirements: > > (1) Be a doctor of medicine, doctor of osteopathy, or doctor of > > podiatric medicine licensed to practice medicine, osteopathy, or > > podiatry in the State in which the laboratory is located or have > earned > > a doctoral, master's or bachelor's degree in a chemical, > physical, > > biological or clinical laboratory science, or medical technology > from an > > accredited institution; > > (2)(i) Have earned an associate degree in a laboratory science, or > > medical laboratory technology from an accredited institution or-- > > (ii) Have education and training equivalent to that specified in > > paragraph (b)(2)(i) of this section that includes-- > > (A) At least 60 semester hours, or equivalent, from an accredited > > institution that, at a minimum, include either-- > > (1) 24 semester hours of medical laboratory technology courses; or > > (2) 24 semester hours of science courses that include-- > > (i) Six semester hours of chemistry; > > (ii) Six semester hours of biology; and > > (iii) Twelve semester hours of chemistry, biology, or medical > > laboratory technology in any combination; and > > (B) Have laboratory training that includes either of the following: > > (1) Completion of a clinical laboratory training program approved > or > > accredited by the ABHES, the CAHEA, or other organization > approved by > > HHS. (This training may be included in the 60 semester hours > listed in > > paragraph (b)(2)(ii)(A) of this section.) > > (2) At least 3 months documented laboratory training in each > > specialty in which the individual performs high complexity testing. > > (3) Have previously qualified or could have qualified as a > technologist under Sec. 493.1491 on or before February 28, 1992 > > On Mon, Apr 23, 2012 at 1:19 PM, Glen Dawson > wrote: > > > > > All, > > > > Can a histotech perform GROSSING if he/she has an associate's degree > > in Histotechnology from an accredited institution (Argosy in MN)? > > > > Any help would be appreciated. > > > > Thank-you, > > > > Glen Dawson BS, HT(ASCP) & QIHC > > Histology Technical Specialist > > Mercy Health System > > Janesville, WI > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From John.McGinley <@t> ColoState.EDU Wed Apr 25 14:24:38 2012 From: John.McGinley <@t> ColoState.EDU (McGinley,John) Date: Wed Apr 25 14:24:42 2012 Subject: [Histonet] ER & PR antibodies for rat tissue Message-ID: <9633BECFEA62114E8318B7754A08866C01F035BC@ex11.colostate.edu> Hi, I've been using Dako 1D5 (ER) and Biogenex PR88 (PR) on rat tissue for a number of years. I was curious if anyone out there working with rat tissue and has found any ER or PR clones that might work better. Has anyone tried rabbit monoclonals on rat tissue, e.g. SP1 (ER) or Y85 (PR)? Thanks, John ----------------------------- John McGinley Cancer Prevention Laboratory Colorado State University From nicole <@t> dlcjax.com Wed Apr 25 14:26:35 2012 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed Apr 25 14:26:40 2012 Subject: [Histonet] RE: Qualifications for grossing In-Reply-To: <2917489537579372361@unknownmsgid> References: <2917489537579372361@unknownmsgid> Message-ID: <2744.208.62.167.196.1335381995.squirrel@webmail.realpages.com> >Im sorry you feel that way about me. There is nothing snide here. I respect your opinion and have no foul words for you. Im happy that you have earned your education. I currently am in school trying to further mine, and I belive an education is so important. To a person and a profession. Have a wonderful day. Nicole Tatum, HT ASCP Try to keep your snide remarks quiet, and respond with some degree of > respect. We will not always agree, but there is no "strike" there. You > dislike me,, that's fine. But keep your personal comments to yourself. > If you can be that mature. > > Sent from my Windows Phone > From: Nicole Tatum > Sent: 4/25/2012 12:09 PM > To: Joanne Clark; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: Qualifications for grossing > Yupp he strikes again. > > > Joanne, > > I strongly agree with your perspective. Many Techs do not have formal > expensive educations and have sat on the bench for many years and > eventually became grandfathered in. Those techs are the life blood of > pathology. It has only been in recent years that licensure has become a > larger part of health care requiring personal to obtain certification to > "hopefully" increase patient care. But, this argument is becoming a thing > of the past, because CLIA, CAP, JOCA have set standards that personal must > meet regardless of the extensive OJT. I am qualified to gross based on > these accrediators standards. It is others opinions that think these > "standards" are weak. If the argument is greed, than people should > understand that employee payroll is the highest cost within a laboratory > so to help cut cost to our bankrupt health care system, why not pay a > Histologist who is clearly qualified to do a job they have been doing > since the beginning of pathology. > > > The pathologists? assistant profession began in 1969 with a pilot training > program at the Veterans Administration Medical Center (VAMC) in Durham, > North Carolina. > > In 1856 William Perkin discovered the dye mauve that was used in the early > 1860s by F W B Benke of Marlbery. Joseph Janavier Woodward, a surgeon in > the US Army, used fuchsine and aniline blue to stain human intestines. > Paul Ehrlich realized that the chemical dyes obtained from coal tar did > not simply color cells but combined with the chemical elements within them > to form new substances. The Swiss chemist Friedrich Miescher, in 1869 used > aniline dyes to examine the cell nucleus. In 1875 Carl Weigart, Ehrlich's > cousin, demonstrated the fuchsine derivative methyl violet stained > bacteria as opposed to tissue. > > The first histologist, Marcello Malpighi (1628-1694), an Italian > anatomist, is in fact considered the true ?Father of Histology?. > > 1819, A. Mayer created the term Histology. In the sequence of the previous > word tissue, made use of two classical Greek root words (histos = tissue > and logos = study > > So, my point is I do believe Histo's have been involved since the very > beginning. We as a profession have a combined experience well beyond that > of any formal education. > > Last thought, and I quote, "I know I would make mistakes, and mistakes may > be considered part of the learning process, but do we really want to > accept that in health care? Mistakes should happen in school, not with a > real, live patients tissue." > > This statement clearly conflicts with all aspect of becoming a medical > professional. Our state/government funded hospitals employ thousands of > residents each year who treat thousands of indigent and paying patients. > This is their school. Histologist do interships within hospitals to get > training. This is their school to. Nurses, MLT's, MA's, everyone in health > care learns the actual (beyond books)trade from watching and working with > skilled persons who have many years of experience. They would not place a > student with a person who has a degree but no experience. My education > qualifies me to be trained by anyone in my field of pathology, and I > should be used where my skills will best serve my department and increase > patient care. Each one of us serves a purpose and is valuable, no matter > what job we perform with what amount of education. The law is weeding out > those who are no longer qualified to work in out field, they set the > standard. Let's let them make the decisions on who is qualified to do what > and stick together to ensure its fair to each one of us. > > Can't we all just get along...hehehehehe > > Nicole Tatum HT, ASCP > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ASelf <@t> georgetownhospitalsystem.org Wed Apr 25 14:38:57 2012 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Wed Apr 25 14:39:07 2012 Subject: [Histonet] Cytology Standards Message-ID: Good Afternoon Histonetters, I need your help. I have been asked to come up with a list of standards for non-gyn cytologies. These standards will be used as a means of evaluating an employee for their annual evaluation. The employee will have to meet - exceed - or not meet the standard depending on their job performance. These standards will help determine their raise percentage. So here I am writing to see if anyone out in histoland has anything that they would be willing to share with me. Any help will be greatly appreciated. Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From mdpraet <@t> gmail.com Wed Apr 25 14:58:13 2012 From: mdpraet <@t> gmail.com (mequita praet) Date: Wed Apr 25 14:58:16 2012 Subject: [Histonet] barcoding Message-ID: Wow, good point, Vinnie! Thanks for the heads up. Mequita Praet From jclark <@t> pcnm.com Wed Apr 25 16:28:23 2012 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Apr 25 16:28:49 2012 Subject: [Histonet] RE: Histonet Digest, Vol 101, Issue 32 In-Reply-To: <20120425170033.B3B0363E8D0@mx10.myoutlookonline.com> References: <20120425170033.B3B0363E8D0@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E3784CB7F759@S10MAILD001N1.SH10.lan> Davide and Rene, you have very valid points and I do not necessarily disagree with you. But the reality is that it is an accepted CAP/CLIA allowed practise and will continue. You both have the right to voice your opinions on the issue, but perhaps histonet which is made up mostly of techs, many of whom gross (not by choice) is not the best place to do it without causing a lot of controversy. You need to take your concerns where they might make a difference, to CAP or CLIA. If you believe in it strongly enough you will try and do something about it. Just know that those of us who do gross, do everything within our power to do the job safely for those patients we serve. Respectfully Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ------------------------------ Message: 14 Date: Wed, 25 Apr 2012 09:34:29 -0700 From: Davide Costanzo Subject: Re: [Histonet] RE: Qualifications for grossing To: Joanne Clark Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Joanna, I wanted to take an opportunity to explain my, and most of my colleagues, feelings about CLIA '88 with respect to grossing standards. But I want to start by stating that this goes both ways, I also do not feel it is appropriate for an ASCP certified PA to be performing Immunohistochemistry, or other stains in the lab. Both histotechnicians (ologists) and PA's have a very clear role in the pathology laboratory. Both have very different training programs. Both HT's and PA's should be protected by law, and rules/regulations for each should be clear. One is not better than the other, and I certainly hope you do not think I have an opinion different from that. Both are highly qualified individuals in their area of expertise. In many states, and I will use Florida as an example because that is what I am familiar with, there are clear definitions in the law as to whom can perform what tasks. In the State of Florida, a PA (regardless of training level) is not to perform frozen sections. That State only allows Pathologists and HT's to cut a frozen. This is the result of much effort put in to changing those rules by the HT's in Florida. Clearly they saw PA's as a threat to their job, and took action. Not a problem, I am happy to let them do the frozen sections. What was it about cutting a frozen section that the HT's thought a PA could not handle? I do not know, but nonetheless they reacted. Certainly PA's are heavily trained in how to cut a frozen section, and it is generally considered our responsibility in most places in the US that I have seen, and I have seen many. Rarely, outside the State of Florida, do I see PA's that do not cut frozens. Now, on to the issue of grossing techs. There are myriad reasons why I, and most of my peers, think it is not appropriate to utilize "grossing techs". For starters, and to be clear, the use of such techs serves one principal purpose to the pathologist's and institutions that employ them - to save money and increase their profits. They are not employed because they represent the clear choice for the utmost in patient care, and to suggest that is not just misleading, but completely false. Grossing small specimens is never just about transferring tissue from a container to a block. Many tend to try and downplay the importance of that task, and overlook things that could be problematic without certain training/skills. And, there are many grossing techs that do larger cases, from gallbladders all the way up to mastectomies and beyond - all with no didactic education, no proficiency testing and no rotations through various types of insitutions. I have never seen a study, but perhaps someone on here has, that points out the sharp increase in error rates found when a tech is used to gross, versus a trained pathologists' assistant. There is a drastic difference. It is distinct, and a study is really not needed to see that difference. Now, to be clear again, that is not to say that every tech that grosses does a bad job. No "vitriol" here. It is just a fact, and a troubling one at that. Imagine the difference in quality you would see if you had me doing all your stains! I am not trained as an HT. You could argue that I could be trained, but do you really want to open that can of worms? Do you want medicine to allow for that, and risk the HT profession? Probably not, and we do not either. Do you think I would be as good as you are, given all the real education you received when getting your HT training? I don't think I would be as good as you are at doing your job. As an example to illustrate, anyone that grosses should know how to answer these very basic questions. These might help shed some light on the issue: 1. What is the most common neoplasm of the gallbladder, what does it look like, and where is it found? Would you know it if you saw it? Is it benign, or malignant? 2. What is the reason that all appendices should have the margin submitted in the initial submission? 3. Would you know the difference between an esophageal bx and a bx from any other part of the GI tract simply by gross appearance? What would you do if you had 2 specimens, one esophagus and one duodenal and they were reversed in the specimen containers by the biopsy tech? Would you be able to pick up on that mistake? 4. How should you handle a skin punch for alopecia? 5. If sent a skin for frozen, and it was a pilomatrixoma, would you know it, and would you still freeze it, or ask the pathologist first to avoid doing an unnecessary frozen? 6. When are tangential margins more appropriate than perpendicular, and vice versa? You can ask me the same types of questions as they pertain to histology, and there is no chance I can answer them as correctly, and with as much clarity as a well trained HT. The bottom line is patient care and quality. I cannot provide the level of quality in histology as you can, and a grossing tech cannot provide that quality at the dissection bench. It just is not possible. The deal is simple - I will never downplay the value of your education and training by suggesting anyone can do it. All I ask is the same in return. Do not suggest that anyone can be trained on the job to do what it took me many years of college to learn, and perform that work at the same level. And I could not do your job nearly as well as you do. I am always impressed with histotechs - they have a great body of knowledge and do a very detailed, intricate and challenging job. For me to suggest, that given a few minutes here and there of training, that I can do your job as well as you would be very condescending. I don't think for one minute you could make me as good as you are by spending just a little bit of time with me. I know I would make mistakes, and mistakes may be considered part of the learning process, but do we really want to accept that in healthcare? Mistakes should happen in school, not with a real, live patients tissue. We should all be aiming to provide the highest level of care possible. I want my surgical specimen (biopsy or other) grossed by a PA, or MD and I want that specimen cut and stained by an ASCP certified HT. Lowering standards is a slippery slope, and one that should not be embarked upon in the world of medicine. I get crucified on this email server constantly. I have read and re-read the above. I see nothing in this to suggest condescension. For those of you that will see that no matter what, it is clearly personal for you, and for that I am sorry. Nothing here is meant to be offensive, just illustrative. On Wed, Apr 25, 2012 at 7:41 AM, Joanne Clark wrote: > > David, after reading your post I was not at all surprised to see that you > are a PA. I am assuming that explains your vitriol towards techs that > gross. Yes, CLIA does provide the educational requirements for high > complexity testing, but what on earth makes you think that a tech with the > proper CLIA qualifications can gross without proper training by a > pathologist? CAP requires that as well as extensive documentation of > training AND a list of the specimens approved by the Lab Director that a > 'non-pathologist' is allowed to gross. I'm sure you can tell that I am a > Histotech with an Associates Degree and I do the grossing in my lab. I can > assure you that I do a good job and if there is EVER any question regarding > how to gross in a specimen I will get a pathologist. To make it clear, > just because we tech's that gross do not have a masters as a pathologist > assistant, we care just as much about the patients we serve as a PA does. > Another point I would like to make is that very often we gross not by > choice but because it is what our pathologists demand of us and they > wouldn't put us there if we couldn't do the job. Believe me, when I say > that I do want to get my masters as a PA, but I haven't been able to find a > program that accommodates someone who is working full time and can not > afford to quit to go back to school. I am currently finishing up my > Bachelors, because I still want to pursue it. > > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > From pathlocums <@t> gmail.com Wed Apr 25 18:17:01 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Wed Apr 25 18:17:06 2012 Subject: [Histonet] RE: Histonet Digest, Vol 101, Issue 32 In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3784CB7F759@S10MAILD001N1.SH10.lan> References: <20120425170033.B3B0363E8D0@mx10.myoutlookonline.com> <0494A7D4E8CC254EA2FB81464982E3784CB7F759@S10MAILD001N1.SH10.lan> Message-ID: Very well spoken. You will note that neither of us raised this subject, but did respond to a post previously entered. And, as I clarified to another person here privately, the problem with CLIA does not just mean we are going after HT's that gross - CLIA allows ANYONE with those very minimal requirements to gross. Most gross techs are not HT's, most are off the street people with the bare bones required by law, that are taught on the job to toss biopsies into cassettes. And, many of these people do much larger cases as well - all legally under CLIA "88. It's a problem, and it is not meant to upset anyone on here. Most of those techs, as I stated, are not HT's and are not on this site anyway. The proper forum of course is to reach out to government officials and try and get that law changed. Perhaps we will. A lot of progress has been made in the area over the years, now we just need to modify the laws to reflect the times within which we live. I did want to point out one thing though - there was a comment about saving money in healthcare, and that was a valid reason to employ a grossing tech. Let's be clear on this subject - there is a BOAT LOAD of money in the technical component of AP services. This is why you see GI docs and Urologists all over the nation opening their own histo labs. They want to cash in. The reimbursement for technical charges is public knowledge - look it up and you will find the Medicare rates for your area very publicly posted by Medicare. It's not a secret. Take those rates, multiply by 80% because even Medicare does not really pay what they say and then multiply it out for your volume. Be sure to include all your 88305's and other standard charges, and add all the fees for your stains, frozen sections, decalcifying, etc. When you see just what Medicare pays your site, then look at your paycheck and ask yourself "Is my lab really suffering financially?" Probably not. And that is based upon Medicare rates, the truth is the lab makes more than that because most insurers pay higher than Medicare. Where does the money go? I don't think I have to tell you. On Wed, Apr 25, 2012 at 2:28 PM, Joanne Clark wrote: > Davide and Rene, you have very valid points and I do not necessarily > disagree with you. But the reality is that it is an accepted CAP/CLIA > allowed practise and will continue. You both have the right to voice your > opinions on the issue, but perhaps histonet which is made up mostly of > techs, many of whom gross (not by choice) is not the best place to do it > without causing a lot of controversy. You need to take your concerns where > they might make a difference, to CAP or CLIA. If you believe in it strongly > enough you will try and do something about it. Just know that those of us > who do gross, do everything within our power to do the job safely for those > patients we serve. > > Respectfully > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > ------------------------------ > > Message: 14 > Date: Wed, 25 Apr 2012 09:34:29 -0700 > From: Davide Costanzo > Subject: Re: [Histonet] RE: Qualifications for grossing > To: Joanne Clark > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset=ISO-8859-1 > > Joanna, > > I wanted to take an opportunity to explain my, and most of my colleagues, > feelings about CLIA '88 with respect to grossing standards. But I want to > start by stating that this goes both ways, I also do not feel it is > appropriate for an ASCP certified PA to be performing Immunohistochemistry, > or other stains in the lab. Both histotechnicians (ologists) and PA's have > a very clear role in the pathology laboratory. Both have very different > training programs. Both HT's and PA's should be protected by law, and > rules/regulations for each should be clear. One is not better than the > other, and I certainly hope you do not think I have an opinion different > from that. Both are highly qualified individuals in their area of > expertise. > > In many states, and I will use Florida as an example because that is what I > am familiar with, there are clear definitions in the law as to whom can > perform what tasks. In the State of Florida, a PA (regardless of training > level) is not to perform frozen sections. That State only allows > Pathologists and HT's to cut a frozen. This is the result of much effort > put in to changing those rules by the HT's in Florida. Clearly they saw > PA's as a threat to their job, and took action. Not a problem, I am happy > to let them do the frozen sections. > > What was it about cutting a frozen section that the HT's thought a PA could > not handle? I do not know, but nonetheless they reacted. Certainly PA's are > heavily trained in how to cut a frozen section, and it is generally > considered our responsibility in most places in the US that I have seen, > and I have seen many. Rarely, outside the State of Florida, do I see PA's > that do not cut frozens. > > Now, on to the issue of grossing techs. There are myriad reasons why I, and > most of my peers, think it is not appropriate to utilize "grossing techs". > For starters, and to be clear, the use of such techs serves one principal > purpose to the pathologist's and institutions that employ them - to save > money and increase their profits. They are not employed because they > represent the clear choice for the utmost in patient care, and to suggest > that is not just misleading, but completely false. > > Grossing small specimens is never just about transferring tissue from a > container to a block. Many tend to try and downplay the importance of that > task, and overlook things that could be problematic without certain > training/skills. And, there are many grossing techs that do larger cases, > from gallbladders all the way up to mastectomies and beyond - all with no > didactic education, no proficiency testing and no rotations through various > types of insitutions. > > I have never seen a study, but perhaps someone on here has, that points out > the sharp increase in error rates found when a tech is used to gross, > versus a trained pathologists' assistant. There is a drastic difference. It > is distinct, and a study is really not needed to see that difference. Now, > to be clear again, that is not to say that every tech that grosses does a > bad job. No "vitriol" here. It is just a fact, and a troubling one at that. > > Imagine the difference in quality you would see if you had me doing all > your stains! I am not trained as an HT. You could argue that I could be > trained, but do you really want to open that can of worms? Do you want > medicine to allow for that, and risk the HT profession? Probably not, and > we do not either. Do you think I would be as good as you are, given all > the real education you received when getting your HT training? I don't > think I would be as good as you are at doing your job. > > As an example to illustrate, anyone that grosses should know how to answer > these very basic questions. These might help shed some light on the issue: > > 1. What is the most common neoplasm of the gallbladder, what does it > look like, and where is it found? Would you know it if you saw it? Is it > benign, or malignant? > 2. What is the reason that all appendices should have the margin > submitted in the initial submission? > 3. Would you know the difference between an esophageal bx and a bx from > any other part of the GI tract simply by gross appearance? What would you > do if you had 2 specimens, one esophagus and one duodenal and they were > reversed in the specimen containers by the biopsy tech? Would you be able > to pick up on that mistake? > 4. How should you handle a skin punch for alopecia? > 5. If sent a skin for frozen, and it was a pilomatrixoma, would you know > it, and would you still freeze it, or ask the pathologist first to avoid > doing an unnecessary frozen? > 6. When are tangential margins more appropriate than perpendicular, and > vice versa? > > You can ask me the same types of questions as they pertain to histology, > and there is no chance I can answer them as correctly, and with as much > clarity as a well trained HT. > > The bottom line is patient care and quality. I cannot provide the level of > quality in histology as you can, and a grossing tech cannot provide that > quality at the dissection bench. It just is not possible. > > The deal is simple - I will never downplay the value of your education and > training by suggesting anyone can do it. All I ask is the same in return. > Do not suggest that anyone can be trained on the job to do what it took > me many years of college to learn, and perform that work at the same level. > And I could not do your job nearly as well as you do. I am always impressed > with histotechs - they have a great body of knowledge and do a very > detailed, intricate and challenging job. For me to suggest, that given a > few minutes here and there of training, that I can do your job as well as > you would be very condescending. I don't think for one minute you could > make me as good as you are by spending just a little bit of time with me. I > know I would make mistakes, and mistakes may be considered part of the > learning process, but do we really want to accept that in healthcare? > Mistakes should happen in school, not with a real, live patients tissue. > > We should all be aiming to provide the highest level of care possible. I > want my surgical specimen (biopsy or other) grossed by a PA, or MD and I > want that specimen cut and stained by an ASCP certified HT. Lowering > standards is a slippery slope, and one that should not be embarked upon in > the world of medicine. > > I get crucified on this email server constantly. I have read and re-read > the above. I see nothing in this to suggest condescension. For those of you > that will see that no matter what, it is clearly personal for you, and for > that I am sorry. Nothing here is meant to be offensive, just illustrative. > > > > > > > > > > > > > > > > On Wed, Apr 25, 2012 at 7:41 AM, Joanne Clark wrote: > > > > > David, after reading your post I was not at all surprised to see that you > > are a PA. I am assuming that explains your vitriol towards techs that > > gross. Yes, CLIA does provide the educational requirements for high > > complexity testing, but what on earth makes you think that a tech with > the > > proper CLIA qualifications can gross without proper training by a > > pathologist? CAP requires that as well as extensive documentation of > > training AND a list of the specimens approved by the Lab Director that a > > 'non-pathologist' is allowed to gross. I'm sure you can tell that I am a > > Histotech with an Associates Degree and I do the grossing in my lab. I > can > > assure you that I do a good job and if there is EVER any question > regarding > > how to gross in a specimen I will get a pathologist. To make it clear, > > just because we tech's that gross do not have a masters as a pathologist > > assistant, we care just as much about the patients we serve as a PA does. > > Another point I would like to make is that very often we gross not by > > choice but because it is what our pathologists demand of us and they > > wouldn't put us there if we couldn't do the job. Believe me, when I say > > that I do want to get my masters as a PA, but I haven't been able to > find a > > program that accommodates someone who is working full time and can not > > afford to quit to go back to school. I am currently finishing up my > > Bachelors, because I still want to pursue it. > > > > Joanne Clark, HT > > Histology Supervisor > > Pathology Consultants of New Mexico > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From jaylundgren <@t> gmail.com Wed Apr 25 20:42:41 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed Apr 25 20:42:46 2012 Subject: [Histonet] RE: Qualifications for grossing In-Reply-To: <2744.208.62.167.196.1335381995.squirrel@webmail.realpages.com> References: <2917489537579372361@unknownmsgid> <2744.208.62.167.196.1335381995.squirrel@webmail.realpages.com> Message-ID: I agree with Renee's post completely. When the motivation is greed, and not patient care, we, as laboratory professionals, should be concerned. I think we should all realize that the regulations are there to protect ALL of our livelihoods, HTs, PAs, MDs, HTLs, and everyone who works in the lab. Patients (insurance companies, the Federal government) would not pay for testing anymore if the results were invalid. If I were a Histology Superintendent thinking of having an under trained laboratory aide do immunohistochemistry, or gross: Could you get away with it?: Sure. Save $40,000. / year per HTL you replace, more if PA. Would you want that to be your specimen? : No. What would one nasty malpractice lawsuit cost your facility?: Potentially multi-millions of dollars. Hiring unqualified techs is false economy. Reimburse your lab aides for tuition if you want them to do high complexity testing. Apologies to any lab aides with PhDs, or any 6th grade dropouts with 30 years of X-ray Crystallography and Proteomics bench experience. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) From luger551 <@t> yahoo.com Wed Apr 25 21:40:57 2012 From: luger551 <@t> yahoo.com (luger551@yahoo.com) Date: Wed Apr 25 21:40:48 2012 Subject: [Histonet] RE: Qualifications for grossing In-Reply-To: References: <2917489537579372361@unknownmsgid> <2744.208.62.167.196.1335381995.squirrel@webmail.realpages.com> Message-ID: <599b114d-b14e-4742-b096-3598a4469715@blur> Wow .....I am appalled with these threads. There are many of us in our field who have grown up in hospital settings learning real time Histotechnolgy and not by books. Sitting bedside with patients speaking with their loved ones. Let's respect this and move on to other topics that challenge us present day in our fields... Regards to ALL of us in our field.... Candace Connected by DROID on Verizon Wireless -----Original message----- From: Jay Lundgren To: Nicole Tatum Cc: histonet@lists.utsouthwestern.edu Sent: Thu, Apr 26, 2012 01:43:38 GMT+00:00 Subject: Re: [Histonet] RE: Qualifications for grossing I agree with Renee's post completely. When the motivation is greed, and not patient care, we, as laboratory professionals, should be concerned. I think we should all realize that the regulations are there to protect ALL of our livelihoods, HTs, PAs, MDs, HTLs, and everyone who works in the lab. Patients (insurance companies, the Federal government) would not pay for testing anymore if the results were invalid. If I were a Histology Superintendent thinking of having an under trained laboratory aide do immunohistochemistry, or gross: Could you get away with it?: Sure. Save $40,000. / year per HTL you replace, more if PA. Would you want that to be your specimen? : No. What would one nasty malpractice lawsuit cost your facility?: Potentially multi-millions of dollars. Hiring unqualified techs is false economy. Reimburse your lab aides for tuition if you want them to do high complexity testing. Apologies to any lab aides with PhDs, or any 6th grade dropouts with 30 years of X-ray Crystallography and Proteomics bench experience. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Thu Apr 26 10:30:22 2012 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Thu Apr 26 10:32:01 2012 Subject: [Histonet] Thanks again Message-ID: I just wanted to say thanks again to all of those on histonet. The questions that are asked go a long way in teaching troubleshooting. Also, a few weeks ago the question was asked about purchasing forceps. I don't remember when or who. One of the suggestions was jewelry forceps from craft stores such as Hobby Lobby. That was a great suggestion!! I took a field trip to Hobby Lobby and found some nice curved forceps with ergonomic finger pads for $3.99. Instead of ordering from Hobby Lobby, I called the distributor and was able to get 15 pairs for $2.99. The tension and the weight of the forceps are good, and have a non-stick coating. We will be placing them in our microtomy kits for next year. For those interested, I also found Collins Scalpels (paraffin knives) at D R Instruments for $6.45 each if anyone needs some. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org From mtitford <@t> aol.com Thu Apr 26 10:40:05 2012 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Thu Apr 26 10:40:21 2012 Subject: [Histonet] Frozen section staining question Message-ID: <8CEF1D9FC011BE3-2114-20D0@Webmail-m115.sysops.aol.com> In our frozen section room, we use plastic dropper bottles (holding about 130ml each) to pour the solutions on the slide on a rack to stain the F/S. We figure you don't risk getting floaters and the solutions are fresh. However, these bottles are "home made" and do not look professional. We have to use tape to label the bottles, and record when they were last filled, and they don't clean easily. Does any company supply a set of professionally labeled dropper bottles for frozen section stainig? I have looked in the regular catalogues (Fisher, etc) but cannot find any there. Thanks in advance Michael Titford USA Pathology Mobile AL USA From rjbuesa <@t> yahoo.com Thu Apr 26 10:48:35 2012 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 26 10:48:44 2012 Subject: [Histonet] Frozen section staining question In-Reply-To: <8CEF1D9FC011BE3-2114-20D0@Webmail-m115.sysops.aol.com> Message-ID: <1335455315.53531.YahooMailClassic@web162102.mail.bf1.yahoo.com> Try Google. You will be surprised how many "unlikely" things you will find! Ren? J. --- On Thu, 4/26/12, mtitford@aol.com wrote: From: mtitford@aol.com Subject: [Histonet] Frozen section staining question To: histonet@lists.utsouthwestern.edu Date: Thursday, April 26, 2012, 11:40 AM In our frozen section room, we use plastic dropper bottles (holding about 130ml each)? to pour the solutions on the slide on a rack to stain the F/S.? We figure you don't risk getting floaters and the solutions are fresh. However, these bottles are? "home made" and do not look professional. We have to use tape to label the bottles, and record when they were last filled, and they don't clean easily. Does any company supply a set of professionally labeled dropper bottles for frozen section stainig? I have looked in the regular catalogues (Fisher, etc) but cannot find any there. Thanks in advance Michael Titford USA Pathology Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Thu Apr 26 11:27:37 2012 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Apr 26 11:27:45 2012 Subject: [Histonet] Thanks again In-Reply-To: References: Message-ID: Texas go! This is the kind of "can do" thinking that made our country so great! Imagine what the taxpayers could save if we multiplied that kind of savings by every department in every large hospital in the US. By the way, my father was a clinical Professor at MD Anderson, Lawrence E. Lundgren, M.D. "The eyes of Texas are upon you, all the livelong day." Sincerely, Jay A. Lundgren, M.S., HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Apr 26 12:05:06 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Apr 26 12:05:20 2012 Subject: [Histonet] Electron Microscopy Postion at UC San Francisco Message-ID: <8D7C2D242DBD45498006B21122072BF8B3F22863@MCINFRWEM003.ucsfmedicalcenter.org> We have a new opening for an Electron Microscopy Technologist at University of California, San Francisco Medical Center. The job description is below. Website: https://careers.ucsfmedicalcenter.org:8443/psp/hcmprd_cg/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_CE.GBL Enter the job code "histology" in the key words box. The job code is 3496. It is labeled "Histotechnologist II - Pathology (HISTOTECHNOLOGIST II)" The EM lab processed about 400 EM cases per year of kidney, muscle, tumor, blood and cilia samples. We also do muscle enzyme histochemistry (200/yr) and immunofluorescence for kidney and skin. Four technologists staff the lab. The lab is located at the main campus near Golden Gate park. If you have any questions at all please contact me. Apply online but send your resume and cover letter to me as well so it doesn't get lost in the system. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org ********************************** Job ID: 3496 Job Title: Histotechnologist II - Pathology (HISTOTECHNOLOGIST II) Job Code: 9066 Department: Pathology-Surgical / Histology Location: Parnassus Full/Part Time: Full-Time Regular/Temporary: Regular Shift: 8-hour Variable Weekly Hours: 40 Union Information: This classification is represented by a union Appointment Type: Career At the University of California San Francisco Medical Center, teamwork and continual learning have maintained our top 10 ranking as one of "America's Best Hospitals" by U.S.News & World Report. For you, UCSF Medical Center is where you will share and discover something new every day with benefits and rewards that will last a lifetime. Job Summary: Under supervision (HT-I) or general supervision (HT-II) by Senior-level technologists, Lead technologist and the Electron Microscopy Supervisor, the incumbent serves as an Electron Microscopy Technologist in the Laboratory for Electron Microscopy and Neuromuscular Special Studies (EM/NM Laboratory). Duties include EM specimen receipt, gross examination, case accessioning in the Department LIS system, tissue processing, thick sectioning, Ultrathin sectioning/staining, electron microscope operation, examination/digital image photomicrography, immunofluorescence microscopy, muscle histochemistry and nerve teasing. Performs quality control and associated documentation, technical validation, and associated record keeping. Other technical duties as assigned may include backup in the STAT Histology lab or grossing lab. The incumbent will rotate between workstations within the lab. Incumbent must be able to work flex work hours as needed to meet Department operational needs and cover work rotations. Required Qualifications: * College degree in a biological science, chemistry or a related field, or equivalent education and experience to include coursework in biological sciences/chemistry and work experience in Electron Microscopy (EM) or related field such as Histotechnology, including experience performing immunofluorescence or muscle histochemistry, or MSA certified in Electron Microscopy. HT I: * Educational requirements or equivalent combination plus one year of work experience in EM or a related field such as Histotechnology performing immunofluorescence or muscle histochemistry as outlined above. * Excellent interpersonal communication skills required. HT II: * Educational requirements plus one year performing diagnostic(clinical) electron microscopy, immunofluorescence and muscle histochemistry procedures at the HT-1 Level, or two years of general EM experience performing all technical components of electron microscopy (TEM) in a comparable laboratory environment within the last five years. * Knowledge of ultrastructural pathology required. * Excellent interpersonal communication skills required. Note: Viable candidates who partially meet the qualifications required may be placed in a HLT-II level position until skill set/work experience criteria is met for the HT-I level. Preferred Qualifications: * Formal training in electron microscopy preferred. * Experience in Immunofluorescence technique preferred. * Experience in muscle histochemistry highly desirable. * Training or experience in Histology / Histotechnology desirable. Required Licenses/Certifications: HT I, II: MSA Certification in Electron Microscopy desirable. From Heidi.Hawthorne <@t> onassignment.com Thu Apr 26 12:18:08 2012 From: Heidi.Hawthorne <@t> onassignment.com (Heidi Hawthorne) Date: Thu Apr 26 12:18:19 2012 Subject: [Histonet] Job Opportunity - Sacramento, CA Message-ID: <519812AD3D64B24C9311DB9774CF73E2F88BE3@oasslcexm03.oaifield.onasgn.com> Hello, We have a short-term contract position for an experienced Histology Lab Tech in Sacramento, CA. Minimum of 5 years of histopathology or related lab experience required. Immunohistochemistry/immunocytochemistry (IHC/ICC) experience required. Advanced working knowledge and hands-on experience in the production of quality histologic slides for microscopic exam by Veterinary Pathologists required as well. Contact me today if you are interested! Heidi Hawthorne Sr. Account Executive On Assignment, Inc. t: (510) 663-8622 c: (510) 435-7326 f: (866) 741-0805 Heidi.Hawthorne@onassignment.com www.onassignment.com NASDAQ: ASGN Partner with On Assignment Healthcare Staffing to keep you at full productivity throughout your EMR conversion. We fill in the gaps when members of your team, or your entire team, are training. People First. Find me on LinkedIn at: http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 From mamawooo <@t> hotmail.com Thu Apr 26 14:08:45 2012 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Thu Apr 26 14:08:49 2012 Subject: [Histonet] Barcoding specimen tracking, lessons you learned In-Reply-To: <-2850437400662915902@unknownmsgid> References: <-2850437400662915902@unknownmsgid> Message-ID: Great Question. No system can eliminate errors, but using a good system with standard process will and has proven eliminated errors. I used the Vantage System in my lab and the only errors we had in several years (about 1 to 2 per year) occurred when staff were not following standard work and single piece flow. These are very important LEAN concepts that work. I can't imagine implementing a bar coding system without using LEAN workflow in a lab. Doing so would be a great waste of money.JanOmaha > From: pathlocums@gmail.com > Date: Mon, 23 Apr 2012 12:23:37 -0700 > To: Timothy.Morken@ucsfmedctr.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Barcoding specimen tracking, lessons you learned > CC: > > See Rich Pucci at UCSF Pathology. He would be a great resource. > > Sent from my Windows Phone > From: Morken, Timothy > Sent: 4/23/2012 10:31 AM > To: Histonet > Subject: [Histonet] Barcoding specimen tracking, lessons you learned > To anyone who has implemented a barcoding/specimen tracking system in > your lab. What lessons did you learn that would make it easier if you > did it over? We're starting the process and I would like to get some > input on things to look out for! > > Thanks for any info and comments! > > Tim Morken > Department of Pathology > UC San Francisco Medical Center > 505 Parnassus Ave, Box 1656 > Room S570 > San Francisco, CA 94132 > > (415) 353-1266 (ph) > (415) 514-3403 (fax) > tim.morken@ucsfmedctr.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Thu Apr 26 15:40:14 2012 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Thu Apr 26 15:40:18 2012 Subject: [Histonet] Email address for Clifford M. Chapman Message-ID: Hello to all in histoland. Does anyone have the email address for Clifford M. Chapman, technical director for Strata Pathology Services. He gave a webinar today on Quality Management for the Histology Lab. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From amosbrooks <@t> gmail.com Thu Apr 26 15:54:08 2012 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Apr 26 15:54:13 2012 Subject: [Histonet] Problems with pAKT. Message-ID: Hi Courtney, We tried it and didn't have much luck. I think this antibody is in need of a lot more development. We abandoned pAKT for panAKT from Epitomics. I am still not 100% content with any of the AKT antibodies, but this was the best we tried. Good Luck, Amos On Wed, Apr 25, 2012 at 7:18 PM, wrote: > Message: 2 > Date: Wed, 25 Apr 2012 13:02:16 -0400 > From: Courtney Pierce > Subject: [Histonet] Problems with pAKT. > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > E596AA0B8A45794A8EF828F5158C44E24B32E47DA8@USADC-AMBXD00.quintiles.net> > > Content-Type: text/plain; charset="us-ascii" > > Has anyone had cross reacting problems with pAKT. I have used both Cell > Signaling #3787 and #4606. Can anyone help me with this. > > Thanks > Courtney > From tkngflght <@t> yahoo.com Thu Apr 26 19:25:01 2012 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Apr 26 19:25:04 2012 Subject: [Histonet] Michael Titford's bottle question Message-ID: <1335486301.2297.YahooMailNeo@web39403.mail.mud.yahoo.com> Hey Michael- ? You can get various sizes with different style dropper?tips VERY affordably on the internet on eBay.? They're inexpensive enough you can pop a paper label on it when you fill it and throw it away when it's empty.? 0.5, 1 and 2 oz bottles as well as larger ml sizes.? You could even get 250 ml wash bottles or just the tubes and?tips if you're buying small plastic premade stain solutoins that fit that size cap. ? Like Toysha finding the forceps at Hobby Lobby - the price is higher when it's marketed for medical: try non-medical suppliers. ? Best wishes! Cheryl Kerry, HT(ASCP) Full Staff Inc. From drmtech09 <@t> gmail.com Thu Apr 26 22:06:34 2012 From: drmtech09 <@t> gmail.com (Eric Baltazar) Date: Thu Apr 26 22:06:39 2012 Subject: [Histonet] Refrigerant for Cryostat Message-ID: Hello Fellas! There's a Cryostat in our inventory requiring a cooling system check up, temp. is dropping, compressor is worked up to reach its optimum cutting level for Mohs cs. Any recommended service company in the Southern California area? I'd appreciate any infos on this issue..Thanks! -- Eric T. Baltazar Dermtech Mohs Services Group Inc. Ofc: (510) 545 -MOHS Cell: (213) 290 -3679 From mervatawad <@t> aol.com Fri Apr 27 05:14:39 2012 From: mervatawad <@t> aol.com (mervatawad@aol.com) Date: Fri Apr 27 05:17:12 2012 Subject: [Histonet] Please do not send any more e-mail. Message-ID: <8CEF275B072CC8A-2224-5452@webmail-m081.sysops.aol.com> Please do not send any more e-mail. From Laurie <@t> blufrogpath.com Fri Apr 27 08:55:01 2012 From: Laurie <@t> blufrogpath.com (Laurie@blufrogpath.com) Date: Fri Apr 27 08:55:07 2012 Subject: [Histonet] Refrigerant for Cryostat Message-ID: <20120427065501.295dc6182df7e5cbb4f32bc101c30dcc.c02ed9346e.wbe@email15.secureserver.net> Hi Eric, I've used Mikron for ye including cryostats. They a are in Vista CA, and their n Laurie Colbert -------- Original Message -------- Subject: [Histonet] Refrigerant f From: Eric Baltazar <[1]drmtech09@gmail.com> Date: Thu, April 26, 2012 8:06 pm To: Hello Fellas! There's a Cryostat in our inventory re cooling system check up, temp. is dropping, compressor is work its optimum cutting level for Mohs cs. Any recommended se in the Southern California area? I'd appreciate any infos issue..Thanks! -- Eric T. Baltazar Dermtech Mohs Ofc: (510) 545 -MOHS Cell: (213) 290 -3679 ___ Histonet mailing list [3]Histonet@lists.utsouthwe [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:drmtech09@gmail.co 2. 3D"mailto:histonet@lists.utsouthwestern.edu" 3. 3D"mailto:Histonet@lists.utsouthwestern.edu" 4. 3D"http://lists.utsouthwestern.edu/mailman/listinf From BDeBrosse-Serra <@t> isisph.com Fri Apr 27 11:45:51 2012 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Fri Apr 27 11:46:06 2012 Subject: [Histonet] Refrigerant for Cryostat In-Reply-To: References: Message-ID: <493CAA64F203E14E8823737B9EE0E25F0921436511@EXCHMB01.isis.local> Mikron. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Baltazar Sent: Thursday, April 26, 2012 8:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refrigerant for Cryostat Hello Fellas! There's a Cryostat in our inventory requiring a cooling system check up, temp. is dropping, compressor is worked up to reach its optimum cutting level for Mohs cs. Any recommended service company in the Southern California area? I'd appreciate any infos on this issue..Thanks! -- Eric T. Baltazar Dermtech Mohs Services Group Inc. Ofc: (510) 545 -MOHS Cell: (213) 290 -3679 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Fri Apr 27 18:02:48 2012 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri Apr 27 18:06:14 2012 Subject: [Histonet] out of office Message-ID: I will be out of the office starting 04/27/2012 and will not return until 04/30/2012. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. From Nancy_Schmitt <@t> pa-ucl.com Mon Apr 30 12:04:22 2012 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Mon Apr 30 12:04:55 2012 Subject: [Histonet] blocks processed in plastic Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36DEE18B@PEITHA.wad.pa-ucl.com> Hi Histonetters- Is there a special blade or angle or directions required for cutting plastic blocks? As always - Thanks for your help Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From ratliffjack <@t> hotmail.com Mon Apr 30 13:43:18 2012 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Apr 30 13:43:22 2012 Subject: [Histonet] blocks processed in plastic In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36DEE18B@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36DEE18B@PEITHA.wad.pa-ucl.com> Message-ID: Hello Nancy! What type of plastic blocks are you wanting to cut? May I also ask what type of specimens are contained within the blocks. Without much information on what specifically you are working with you are looking at needing diamond knives, tungsten-carbide knives, or diamond impregnated wire, band saw blades, or disc wheels. Best, Jack Jack Ratliff Senior Histologist, BioMimetic Therapeutics, Inc. Chairman, Hard Tissue Committee - National Society for Histotechnology > From: Nancy_Schmitt@pa-ucl.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 30 Apr 2012 17:04:22 +0000 > Subject: [Histonet] blocks processed in plastic > > Hi Histonetters- > Is there a special blade or angle or directions required for cutting plastic blocks? > As always - Thanks for your help > Nancy > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Mon Apr 30 14:18:46 2012 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Apr 30 14:19:06 2012 Subject: [Histonet] Cassette printer comparison Message-ID: <8D7C2D242DBD45498006B21122072BF8B477E509@MCINFRWEM003.ucsfmedicalcenter.org> Hi all, We are looking at cassette printers and have narrowed to three. The printers will be used for 2D code printing so I am wondering what experience anyone has had with reliability of 2D code readability and durability with the output of these three printers Thermo Printmate General Data Leica IP-C Thanks for any info you can give! Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org From pathlocums <@t> gmail.com Mon Apr 30 15:09:17 2012 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Mon Apr 30 15:09:20 2012 Subject: [Histonet] Cassette printer comparison Message-ID: <-3998100205982320642@unknownmsgid> The General Data is nice, but takes up space with its vacuum canister, which they rarely show in pictures. Be sure you see it and make certain it will fit in the space you have. Cassettes are more costly as they are all pre-printed with black facing, but look great. Thermo is less bulky (no vacuum and waste container to clean) but produces a lower quality print. Cassettes are much cheaper, but they have had some troubles with the print head not lasting long. The Leica is not much better (if at all) than the Thermo with respect to print quality. It also constantly jams (the ones I have used). Personally, I would choose the General Data. Costs more in the long term with pricey cassettes, but looks great! Less troubles with barcode scanning too. There is no comparison between this and the other two. Like a Kia vs a Bentley. I just bought the Thermo, and only because my lab is a Thermo Showcase lab and that is all we use, across the board. I wish I could have the General Data unit. Sent from my Windows Phone From: Morken, Timothy Sent: 4/30/2012 12:19 PM To: Histonet Subject: [Histonet] Cassette printer comparison Hi all, We are looking at cassette printers and have narrowed to three. The printers will be used for 2D code printing so I am wondering what experience anyone has had with reliability of 2D code readability and durability with the output of these three printers Thermo Printmate General Data Leica IP-C Thanks for any info you can give! Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Mon Apr 30 16:24:41 2012 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Mon Apr 30 16:24:50 2012 Subject: [Histonet] Productivity Reports Message-ID: Hello to all in histoland. Does any one have to do productivity reports for their boss. If so, what are you using for your unit of measure to say if your techs are being productive. My boss is really into the numbers, graphs and charts. I have a my techs fill out a workload recording form. I really don't know where to begin trying to pull data from it for a report. If anyone has any insight to this and has a report that they would be willing to share, I would be eternally grateful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. 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