[Histonet] TEM using "difficult" tissue

[Daphne Gill]

Hi
I'd like to try some TEM work on rat brain hippocampus. However, the
tissue is less than ideal. Does anyone have any information/insight on
using old (harvested ~2 years ago) tissue that has been subjected to 20
minutes in sodium sulfide (0.4%), fixed using 30% sucrose in 10% BNF for
48 hours, and then frozen? 
Thanks very much for any help you can give me. 
 
Daphne