From Finlay.Finlay <@t> glasgow.ac.uk Thu Sep 1 03:57:28 2011 From: Finlay.Finlay <@t> glasgow.ac.uk (Finlay Finlay) Date: Thu Sep 1 03:57:35 2011 Subject: [Histonet] Armand solution Message-ID: Hello all, Does anyone know of a supplier of Armand solution in the UK or, failing that, a method for making my own. Thank you Finlay From rachel.walls <@t> advocatehealth.com Thu Sep 1 06:10:45 2011 From: rachel.walls <@t> advocatehealth.com (Walls, Rachel) Date: Thu Sep 1 06:11:24 2011 Subject: [Histonet] S100, NEUROFILAMENT, AND CD10 Message-ID: I would like to know what protocols people are using for S100 (m) from Ventana and Neurofilament from Ventana and CD10 from Ventana on the Bench XT using ultaview DAB. Thanks, Rachel. This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. From HornHV <@t> archildrens.org Thu Sep 1 08:03:02 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Sep 1 08:03:10 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> Message-ID: <25A4DE08332B19499904459F00AAACB719A93F37E3@EVS1.archildrens.org> We have a Leica stainer and coverslipper. We like them both. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 8:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper.? Thank you. ? Ernestine Middleton, Manager,? HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emiddlet@montefiore.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From bakevictoria <@t> gmail.com Thu Sep 1 08:06:53 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Sep 1 08:07:01 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> Message-ID: Ventana Symphony - it has a lot of "issues" which Ventana has not completely addressed. On Aug 31, 2011 9:24 PM, "Ernestine Middleton" wrote: > Hi; > Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. > Thank you. > > Ernestine Middleton, Manager, HT, HTL ,BS ,MPA > Montefiore Medical Center > Bronx, New York > 718-920-4157 > emiddlet@montefiore.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christiegowan <@t> msn.com Thu Sep 1 08:14:21 2011 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Thu Sep 1 08:14:24 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> Message-ID: We currently use the Symphony from Ventana and the Leica with attached glass coverslipper. We are happy with both. We use the Leica to do research and the Symphony to do all routine clinical work. > Date: Wed, 31 Aug 2011 18:23:54 -0700 > From: ernestinemiddleton@yahoo.ca > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) > > Hi; > Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. > Thank you. > > Ernestine Middleton, Manager, HT, HTL ,BS ,MPA > Montefiore Medical Center > Bronx, New York > 718-920-4157 > emiddlet@montefiore.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Thu Sep 1 08:27:06 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Sep 1 08:27:20 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <25A4DE08332B19499904459F00AAACB719A93F37E3@EVS1.archildrens.org> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> <25A4DE08332B19499904459F00AAACB719A93F37E3@EVS1.archildrens.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F54FC7@SMCMAIL01.somerset-healthcare.com> We have the Leica ST5020 and CV5030. We like them both. The stainer has the ability to run multiple protocols, and clips placed on the staining rack are linked to the different programs - so no manual selection. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, September 01, 2011 9:03 AM To: 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) We have a Leica stainer and coverslipper. We like them both. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 8:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. Thank you. ? Ernestine Middleton, Manager,? HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emiddlet@montefiore.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. 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From JWeems <@t> sjha.org Thu Sep 1 08:35:43 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Sep 1 08:35:49 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8B221@CHEXCMS10.one.ads.che.org> We have Leica - it does a great job since they got the bugs worked out and user issues resolved! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 21:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. Thank you. ? Ernestine Middleton, Manager,? HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emiddlet@montefiore.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From PAMarcum <@t> uams.edu Thu Sep 1 08:36:01 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Sep 1 08:36:10 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <25A4DE08332B19499904459F00AAACB719A93F37E3@EVS1.archildrens.org> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> <25A4DE08332B19499904459F00AAACB719A93F37E3@EVS1.archildrens.org> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32035116F9@Mail2Node2.ad.uams.edu> Have two Leica H&E stainers and a coverslipper we love. We automated the PAS for the Leica as we do a large number of bone marrows on the night shift. We are attempting to get another coverslipper and transfer stations for both of coverslippers to the stainers this year. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, September 01, 2011 8:03 AM To: 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) We have a Leica stainer and coverslipper. We like them both. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 8:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper.? Thank you. ? Ernestine Middleton, Manager,? HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emiddlet@montefiore.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From JWeems <@t> sjha.org Thu Sep 1 08:36:32 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Sep 1 08:36:38 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8B222@CHEXCMS10.one.ads.che.org> Oops = one more thing, we have found that clipped corner slides work well. The 90 degree ones hang up.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 21:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. Thank you. ? Ernestine Middleton, Manager,? HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emiddlet@montefiore.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From b-frederick <@t> northwestern.edu Thu Sep 1 09:06:44 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Sep 1 09:06:49 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <25A4DE08332B19499904459F00AAACB719A93F37E3@EVS1.archildrens.org> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> <25A4DE08332B19499904459F00AAACB719A93F37E3@EVS1.archildrens.org> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E13D3B5@evcspmbx3.ads.northwestern.edu> Same here. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, September 01, 2011 8:03 AM To: 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) We have a Leica stainer and coverslipper. We like them both. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 8:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. Thank you. ? Ernestine Middleton, Manager,? HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emiddlet@montefiore.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From deliacs <@t> umdnj.edu Thu Sep 1 09:08:20 2011 From: deliacs <@t> umdnj.edu (Delia, Catherine) Date: Thu Sep 1 09:08:28 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E13D3B5@evcspmbx3.ads.northwestern.edu> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> <25A4DE08332B19499904459F00AAACB719A93F37E3@EVS1.archildrens.org> <62C639732D3F274DACED033EBDF6ADAF1E13D3B5@evcspmbx3.ads.northwestern.edu> Message-ID: <6596937D8D41DF4D9E30D825670BDBB55059241EC6@UMDEXMBX02.core.umdnj.edu> Same here. Catherine Susan Delia, BS., HT. ASCP Chief Technologist, Anatomic Pathology UMDNJ-University Hospital 150 Bergen Street E-151 Newark, New Jersey 07103 Phone: 973-972-5717 Cell: 908-391-1060 Fax: 973-972-5724 deliacs@umdnj.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, September 01, 2011 10:07 AM To: Horn, Hazel V; 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) Same here. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, September 01, 2011 8:03 AM To: 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) We have a Leica stainer and coverslipper. We like them both. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 8:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. Thank you. ? Ernestine Middleton, Manager,? HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emiddlet@montefiore.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Sep 1 09:14:24 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Sep 1 09:14:30 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8B222@CHEXCMS10.one.ads.che.org> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> <92AD9B20A6C38C4587A9FEBE3A30E1640827A8B222@CHEXCMS10.one.ads.che.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8B240@CHEXCMS10.one.ads.che.org> And one more thing... Leica is when your pathologists want glass - Sakura is great for tape.. My mind is working in baby steps today!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, September 01, 2011 09:37 To: Ernestine Middleton; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) Oops = one more thing, we have found that clipped corner slides work well. The 90 degree ones hang up.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 21:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. Thank you. ? Ernestine Middleton, Manager,? HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emiddlet@montefiore.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From deliacs <@t> umdnj.edu Thu Sep 1 09:15:42 2011 From: deliacs <@t> umdnj.edu (Delia, Catherine) Date: Thu Sep 1 09:15:50 2011 Subject: [Histonet] RE: Histotechnologist Position in New Jersey In-Reply-To: <6596937D8D41DF4D9E30D825670BDBB55059241EC6@UMDEXMBX02.core.umdnj.edu> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> <25A4DE08332B19499904459F00AAACB719A93F37E3@EVS1.archildrens.org> <62C639732D3F274DACED033EBDF6ADAF1E13D3B5@evcspmbx3.ads.northwestern.edu> <6596937D8D41DF4D9E30D825670BDBB55059241EC6@UMDEXMBX02.core.umdnj.edu> Message-ID: <6596937D8D41DF4D9E30D825670BDBB55059241EC7@UMDEXMBX02.core.umdnj.edu> Dear Group Members, Please be advised that there is a Histotechnologist Position open at University Hospital-UMDNJ in Newark, New Jersey. If you know anyone who might be interested in joining a great team in the histology lab at University Hospital, please tell them to apply online as soon as possible at www.umdnj.edu/hrweb. Thank you. Catherine Susan Delia, BS., HT. ASCP Chief Technologist, Anatomic Pathology UMDNJ-University Hospital 150 Bergen Street E-151 Newark, New Jersey 07103 Phone: 973-972-5717 Cell: 908-391-1060 Fax: 973-972-5724 deliacs@umdnj.edu From settembr <@t> umdnj.edu Thu Sep 1 09:30:32 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Thu Sep 1 09:30:40 2011 Subject: [Histonet] RE: CAP slides In-Reply-To: References: Message-ID: Her-2 IHC Dana Settembre -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Coppin, Margaret Sent: Wednesday, August 31, 2011 5:53 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP slides Hello, A few months back, there was a discussion on the list about CAP Proficiency Test slides that weren't staining as expected. Some of you had even contacted CAP and they acknowledged that there had been an issue. Any of you whose PT was affected, do you mind jogging my memory as to which PT's were the problem batch? Thanks in advance as always. Margaret ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thiggins <@t> cddmedical.com Thu Sep 1 11:22:30 2011 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Thu Sep 1 11:22:39 2011 Subject: [Histonet] IHC validation Message-ID: <006601cc68c3$5b0d4710$e001a8c0@cdd.loc> Hello Histo Friends, Has anyone had to validate IHC antibodies when you do not have tissue processed in house to use for your validation. I do have control tissue that stains properly but it was acquired from outsides sources with verified proper predicted staining. If you have done this type of validation, how did you make the pathologist feel comfortable with it? I understand pathologist all to well and you can't make them do anything they do not want to do but I am looking for suggestions that might have worked in helping them feel comfortable. Any information would be appreciated. Thanks, Tim From rjbuesa <@t> yahoo.com Thu Sep 1 11:35:06 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 1 11:35:10 2011 Subject: [Histonet] IHC validation In-Reply-To: <006601cc68c3$5b0d4710$e001a8c0@cdd.loc> Message-ID: <1314894906.33422.YahooMailClassic@web65715.mail.ac4.yahoo.com> I had never done that and it would be necessary only if the tissues you are using were fixed in a different way to yours. Tissue fixation is the step that could affect reactivity because if the tissue has been fixed in an alcoholic fixative it would not need HIER and it is uncertain how this unnecessary step (in that specific case) could affect reactivity. No other step (dehydration and clearing) could really affect the reactivity. Even temperatures above 45?C have been demonstrated not to affect the epitopes. Xylene do have an extracting effect on epitopes and it would be nice to know if the clearing agent was xylene. Now, if you fix with NBF, dehydrate with ethanol and clear with xylene and the tissue you have from outside has been processed with a similar protocol, you do not need to validate that tissue. At least is how I see it. Ren? J. --- On Thu, 9/1/11, Tim Higgins wrote: From: Tim Higgins Subject: [Histonet] IHC validation To: histonet@lists.utsouthwestern.edu Date: Thursday, September 1, 2011, 12:22 PM Hello Histo Friends, Has anyone had to validate IHC antibodies when you do not have tissue processed in house to use for your validation.? I do have control tissue that stains properly but it was acquired from outsides sources with verified proper predicted staining. If you have done this type of validation, how did you make the pathologist feel comfortable with it?? I understand pathologist all to well and you can't make them do anything they do not want to do but I am looking for suggestions that might have worked in helping them feel comfortable.? Any information would be appreciated. Thanks, Tim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Sep 1 12:09:22 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Sep 1 12:09:35 2011 Subject: [Histonet] IHC validation In-Reply-To: <006601cc68c3$5b0d4710$e001a8c0@cdd.loc> References: <006601cc68c3$5b0d4710$e001a8c0@cdd.loc> Message-ID: <8D7C2D242DBD45498006B21122072BF84940D495@MCINFRWEM003.ucsfmedicalcenter.org> Tim Use outside controls to validate your own internal controls. Normal control tissue is acceptable and is useful because it has consistent expression of the antigen. Cancer tissue is quite variable in expression and sometimes the expected expression is less than the normal tissue, and sometimes even negative. As a parallel validation test you can send your unstained slides to a second lab that does the test and they send their slides to you. If both results are the same you can feel confident in your test. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Thursday, September 01, 2011 9:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC validation Hello Histo Friends, Has anyone had to validate IHC antibodies when you do not have tissue processed in house to use for your validation. I do have control tissue that stains properly but it was acquired from outsides sources with verified proper predicted staining. If you have done this type of validation, how did you make the pathologist feel comfortable with it? I understand pathologist all to well and you can't make them do anything they do not want to do but I am looking for suggestions that might have worked in helping them feel comfortable. Any information would be appreciated. Thanks, Tim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosie_scrimizzi <@t> hotmail.com Thu Sep 1 13:03:39 2011 From: rosie_scrimizzi <@t> hotmail.com (Rosie Scrimizzi) Date: Thu Sep 1 13:03:45 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8B240@CHEXCMS10.one.ads.che.org> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com>, <92AD9B20A6C38C4587A9FEBE3A30E1640827A8B222@CHEXCMS10.one.ads.che.org>, <92AD9B20A6C38C4587A9FEBE3A30E1640827A8B240@CHEXCMS10.one.ads.che.org> Message-ID: Sakura also do a great glass coverslipper (g2) that links to the prisma stainer....... > From: JWeems@sjha.org > To: JWeems@sjha.org; ernestinemiddleton@yahoo.ca; histonet@lists.utsouthwestern.edu > Date: Thu, 1 Sep 2011 10:14:24 -0400 > Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) > CC: > > And one more thing... Leica is when your pathologists want glass - Sakura is great for tape.. > > My mind is working in baby steps today!! > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce > Sent: Thursday, September 01, 2011 09:37 > To: Ernestine Middleton; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) > > Oops = one more thing, we have found that clipped corner slides work well. The 90 degree ones hang up.. > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton > Sent: Wednesday, August 31, 2011 21:24 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) > > Hi; > Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. Thank you. > > Ernestine Middleton, Manager, HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York > 718-920-4157 > emiddlet@montefiore.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpowers <@t> dpspa.com Thu Sep 1 13:09:03 2011 From: mpowers <@t> dpspa.com (Marian Powers) Date: Thu Sep 1 13:09:11 2011 Subject: [Histonet] Kappa/Lambda ISH Message-ID: Hi: Is anyone running the new Kappa and Lambda ASRs on the Ventana Benchmark and would be willing to share the protocols? Thanks in advance, -- *Marian L. Powers* *Q*uality *L*aboratory *C*onsultants *Doctors Pathology Services * ** c| 302.747.0580 o| 302.677.0000 ext: 110 f | 302.677.0010 From liz <@t> premierlab.com Thu Sep 1 13:08:32 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Sep 1 13:09:31 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA08AF@SBS2K8.premierlab.local> We also have the Sakura Glass coverslipper, it works well, there are some tiny issues, but I suspect all glass coverslippers would have them. If it gets too hot and humid the coverglass will stick and we will have two coverslips on one slide. Also the countertop needs to be level. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosie Scrimizzi Sent: Thursday, September 01, 2011 12:04 PM To: jweems@sjha.org; ernestinemiddleton@yahoo.ca; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) Sakura also do a great glass coverslipper (g2) that links to the prisma stainer....... > From: JWeems@sjha.org > To: JWeems@sjha.org; ernestinemiddleton@yahoo.ca; histonet@lists.utsouthwestern.edu > Date: Thu, 1 Sep 2011 10:14:24 -0400 > Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) > CC: > > And one more thing... Leica is when your pathologists want glass - Sakura is great for tape.. > > My mind is working in baby steps today!! > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce > Sent: Thursday, September 01, 2011 09:37 > To: Ernestine Middleton; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) > > Oops = one more thing, we have found that clipped corner slides work well. The 90 degree ones hang up.. > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton > Sent: Wednesday, August 31, 2011 21:24 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) > > Hi; > Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. Thank you. > > Ernestine Middleton, Manager, HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York > 718-920-4157 > emiddlet@montefiore.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Thu Sep 1 13:36:46 2011 From: mward <@t> wakehealth.edu (Martha Ward) Date: Thu Sep 1 13:36:57 2011 Subject: [Histonet] Test email after email address change Message-ID: Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 From Diane.Craft <@t> amcny.org Thu Sep 1 14:44:37 2011 From: Diane.Craft <@t> amcny.org (Diane.Craft@amcny.org) Date: Thu Sep 1 14:38:01 2011 Subject: [Histonet] Dako's Ki67 MIB1 antibody Message-ID: Has anyone noticed a change in his/her working dilution since Dako changed their Ki67 MIB1 IgG concentrate from 80mg/L to 35mg/L? Diane Craft Pathology Department Animal Medical Center 510 East 62nd St New York NY 10065-8314 212-329-8675 (phone) 212-759-5878 (fax) From nicole <@t> dlcjax.com Thu Sep 1 15:25:58 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Thu Sep 1 15:26:55 2011 Subject: [Histonet] Pathlogix software Message-ID: <3077.208.62.167.196.1314908758.squirrel@webmail.realpages.com> Hello Histos, Im looking for a women I spoke with in reguards to Pathlogix software. She helped me navigate the system..I need you please contact me ASAP. Nicole Tatum 904-400-5902 From BDeBrosse-Serra <@t> isisph.com Thu Sep 1 15:38:04 2011 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Thu Sep 1 15:38:27 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> Message-ID: <493CAA64F203E14E8823737B9EE0E25F0900642E81@EXCHMB01.isis.local> Leica.......all the way! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper.? Thank you. ? Ernestine Middleton, Manager,? HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emiddlet@montefiore.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.Fleming <@t> allina.com Thu Sep 1 15:41:26 2011 From: Jackie.Fleming <@t> allina.com (Fleming, Jackie M) Date: Thu Sep 1 15:41:30 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <493CAA64F203E14E8823737B9EE0E25F0900642E81@EXCHMB01.isis.local> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com>, <493CAA64F203E14E8823737B9EE0E25F0900642E81@EXCHMB01.isis.local> Message-ID: <2342C8D836A0954199F6D58A44BDD1BE0DD9FD@XCHMB01.allina.com> Must use the Leica coverslips - OR it's a lot of downtime! Stainer is slower than we had hoped, but we have a really high volume. Great service! Jackie Fleming HT ASCP Allina Medical Laboratries Histology Technical Consultant phone: 612-863-4773 pager: 612-654-2135 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bea DeBrosse-Serra [BDeBrosse-Serra@isisph.com] Sent: Thursday, September 01, 2011 3:38 PM To: 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) Leica.......all the way! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. Thank you. Ernestine Middleton, Manager, HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emiddlet@montefiore.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. From Wanda.Smith <@t> HCAhealthcare.com Thu Sep 1 16:06:09 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Thu Sep 1 16:06:18 2011 Subject: [Histonet] South Carolina's State Meeting for Histotechnology is November 4-5, 2011 Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA272DD8104B@NADCWPMSGCMS03.hca.corpad.net> Good Afternoon to All!!! The SC Society of Histotechnology (SCSHT) will be having our Fall meeting on November 4-5, 2011 at the beautiful Marina Inn at Grande Dunes in Myrtle Beach, SC!!!!! Everyone is welcome to join us for Fun, Fellowship and Education!!! CEU's will be accredited through NSH. Gayle Callis will be presenting 2 workshops and the Saturday morning session will be "Lateral Violence in Healthcare" . This is a very informative session with group participation!!!! Please email me at wanda.smith@hcahealthcare.com or JLINDA@clemson.edu for an information brochure and hotel accommodations. This is an election year for us so, SC HISTOTECHNOLOGY SOCIETY WE NEED YOU OUT IN FULL FORCE!!!!! Vendors who would like to participate by renting a vendor booth may contact Linda Jenkins by email at JLINDA@clemson.edu for information and an application. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From jqb7 <@t> cdc.gov Fri Sep 2 04:48:37 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Fri Sep 2 04:48:53 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <2342C8D836A0954199F6D58A44BDD1BE0DD9FD@XCHMB01.allina.com> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com>, <493CAA64F203E14E8823737B9EE0E25F0900642E81@EXCHMB01.isis.local> <2342C8D836A0954199F6D58A44BDD1BE0DD9FD@XCHMB01.allina.com> Message-ID: We use high quality slides from Mercedes Medical with no problems at all. We also use traditional slides....no rounded corners and have no problems. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fleming, Jackie M Sent: Thursday, September 01, 2011 4:41 PM To: Bea DeBrosse-Serra; 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) Must use the Leica coverslips - OR it's a lot of downtime! Stainer is slower than we had hoped, but we have a really high volume. Great service! Jackie Fleming HT ASCP Allina Medical Laboratries Histology Technical Consultant phone: 612-863-4773 pager: 612-654-2135 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bea DeBrosse-Serra [BDeBrosse-Serra@isisph.com] Sent: Thursday, September 01, 2011 3:38 PM To: 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) Leica.......all the way! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. Thank you. Ernestine Middleton, Manager, HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emiddlet@montefiore.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nacaela.Johnson <@t> USONCOLOGY.COM Fri Sep 2 07:36:25 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Fri Sep 2 07:37:29 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <2342C8D836A0954199F6D58A44BDD1BE0DD9FD@XCHMB01.allina.com> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com>, <493CAA64F203E14E8823737B9EE0E25F0900642E81@EXCHMB01.isis.local> <2342C8D836A0954199F6D58A44BDD1BE0DD9FD@XCHMB01.allina.com> Message-ID: <71882EED22A283429E8424513A22922D5953CB@txhous1eb015.uson.usoncology.int> We use Mercedes slides and coverslips and have not had any downtime with them. The Leica coverslipper is much more dependable than the sakura G2. I've used both and had to constantly work with the G2. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fleming, Jackie M Sent: Thursday, September 01, 2011 3:41 PM To: Bea DeBrosse-Serra; 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) Must use the Leica coverslips - OR it's a lot of downtime! Stainer is slower than we had hoped, but we have a really high volume. Great service! Jackie Fleming HT ASCP Allina Medical Laboratries Histology Technical Consultant phone: 612-863-4773 pager: 612-654-2135 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bea DeBrosse-Serra [BDeBrosse-Serra@isisph.com] Sent: Thursday, September 01, 2011 3:38 PM To: 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) Leica.......all the way! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. Thank you. Ernestine Middleton, Manager, HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emiddlet@montefiore.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From histotech <@t> imagesbyhopper.com Fri Sep 2 07:44:09 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Sep 2 07:44:19 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com> <493CAA64F203E14E8823737B9EE0E25F0900642E81@EXCHMB01.isis.local> <2342C8D836A0954199F6D58A44BDD1BE0DD9FD@XCHMB01.allina.com> Message-ID: <7FA80E6A-0D4F-4C0B-A606-28653E1A042C@imagesbyhopper.com> We use the Leica autostainer, bridge & coverslipper. We use the premimum coverslips from Fisher and seldom have any issues with them sticking together (and we're in FL!) Does anyone have issues with the coverslipper breaking off the "ears" on the grey/black racks? I have a cupboard "graveyard" for my broken racks. :o( Michelle Sent from my iPhone On Sep 2, 2011, at 5:48 AM, "Bartlett, Jeanine (CDC/OID/NCEZID)" wrote: > We use high quality slides from Mercedes Medical with no problems at all. We also use traditional slides....no rounded corners and have no problems. > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fleming, Jackie M > Sent: Thursday, September 01, 2011 4:41 PM > To: Bea DeBrosse-Serra; 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) > > Must use the Leica coverslips - OR it's a lot of downtime! > Stainer is slower than we had hoped, but we have a really high volume. > Great service! > > Jackie Fleming HT ASCP > Allina Medical Laboratries > Histology Technical Consultant > phone: 612-863-4773 > pager: 612-654-2135 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bea DeBrosse-Serra [BDeBrosse-Serra@isisph.com] > Sent: Thursday, September 01, 2011 3:38 PM > To: 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) > > Leica.......all the way! > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 1896 Rutherford Road > Carlsbad, CA 92008 > 760-603-2371 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton > Sent: Wednesday, August 31, 2011 6:24 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) > > Hi; > Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. > Thank you. > > Ernestine Middleton, Manager, HT, HTL ,BS ,MPA > Montefiore Medical Center > Bronx, New York > 718-920-4157 > emiddlet@montefiore.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Rcartun <@t> harthosp.org Fri Sep 2 09:11:38 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 2 09:11:48 2011 Subject: [Histonet] IHC for TSPY Message-ID: <4E60ABD9.7400.0077.1@harthosp.org> Does anyone offer immunohistochemical testing for the "Y-encoded TSPY protein" in testicular biopsy tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From flnails <@t> texaschildrens.org Fri Sep 2 09:51:15 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Sep 2 09:51:30 2011 Subject: [Histonet] Leica Bond III In-Reply-To: References: Message-ID: I want to poll Leica Bond III users to see if they are able to successfully run EBER Insitu on their unit? We continue to get inconsistent staining and inconsistent background staining. I have been told it's a pH problem, it's a tissue processing problem but we are able to do the stain with great results by hand. I welcome any and all suggestions ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From Holly.Dorsey <@t> carolinashealthcare.org Fri Sep 2 11:15:20 2011 From: Holly.Dorsey <@t> carolinashealthcare.org (Dorsey, Holly L) Date: Fri Sep 2 11:15:27 2011 Subject: [Histonet] New Ventana Kappa and Lambda Probes Message-ID: <254BF6FE0097484DB7EE4A68450964CE09363F501D@CHS-XCHG-VS-06.Carolinas.org> Was wondering if anyone would be willing to share their protocols for the Ventana Kappa and Lambda ASR probes for the Benchmark XT. I have been having a time trying to get these protocols worked out. When I ran the probes the first couple of times with the same protocol as the old probes, they were beautiful. The probes then stopped working all together with no staining at all. I adjusted the stringency wash temp and now I have a ton of background staining. Any suggestions would be greatly appreicated. Thanks, Holly L. Dorsey, HT CMC-NE, Concord NC ________________________________ This electronic message may contain information that is confidential and/or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you. From ross <@t> premierlab.com Fri Sep 2 11:29:25 2011 From: ross <@t> premierlab.com (Ross Benik) Date: Fri Sep 2 11:33:32 2011 Subject: [Histonet] Dako's Ki67 MIB1 antibody In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBB6487@SBS2K8.premierlab.local> Diane, Looks like it did change. Lot# 00004924: 80 mg/L Lot# 00065742: 35 mg/L ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diane.Craft@amcny.org [Diane.Craft@amcny.org] Sent: Thursday, September 01, 2011 1:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako's Ki67 MIB1 antibody Has anyone noticed a change in his/her working dilution since Dako changed their Ki67 MIB1 IgG concentrate from 80mg/L to 35mg/L? Diane Craft Pathology Department Animal Medical Center 510 East 62nd St New York NY 10065-8314 212-329-8675 (phone) 212-759-5878 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Sep 2 12:11:55 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Sep 2 12:12:21 2011 Subject: [Histonet] Dako's Ki67 MIB1 antibody In-Reply-To: <14E2C6176416974295479C64A11CB9AE1DECBB6487@SBS2K8.premierlab.local> References: , <14E2C6176416974295479C64A11CB9AE1DECBB6487@SBS2K8.premierlab.local> Message-ID: Did anyone notice a change in the working dilution of the antibody? Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ross Benik [ross@premierlab.com] Sent: Friday, September 02, 2011 12:29 PM To: Diane.Craft@amcny.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako's Ki67 MIB1 antibody Diane, Looks like it did change. Lot# 00004924: 80 mg/L Lot# 00065742: 35 mg/L ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diane.Craft@amcny.org [Diane.Craft@amcny.org] Sent: Thursday, September 01, 2011 1:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako's Ki67 MIB1 antibody Has anyone noticed a change in his/her working dilution since Dako changed their Ki67 MIB1 IgG concentrate from 80mg/L to 35mg/L? Diane Craft Pathology Department Animal Medical Center 510 East 62nd St New York NY 10065-8314 212-329-8675 (phone) 212-759-5878 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Fri Sep 2 14:35:00 2011 From: mward <@t> wakehealth.edu (Martha Ward) Date: Fri Sep 2 14:35:25 2011 Subject: [Histonet] RE: Leica Bond III In-Reply-To: References: Message-ID: We sometimes have issues with EBER and more often, with the Kappa and Lambda ISH on the Bond 3, more specifically on our tissues that were fixed in B Plus rather than formalin. I would interested in the responses as well. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, September 02, 2011 10:51 AM To: 'Diane.Craft@amcny.org'; histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond III I want to poll Leica Bond III users to see if they are able to successfully run EBER Insitu on their unit? We continue to get inconsistent staining and inconsistent background staining. I have been told it's a pH problem, it's a tissue processing problem but we are able to do the stain with great results by hand. I welcome any and all suggestions ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Fri Sep 2 15:58:51 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Fri Sep 2 15:58:53 2011 Subject: [Histonet] gross room cleaning In-Reply-To: References: Message-ID: <5A33C952BB67F4468AF1F36D739212BC01937C@JERRY.Gia.com> Hey, do y'all wash down your grossing table and soak your utensils at the end of each day? What about the disposable knives....do you throw them away after each patient or at the end of each day? Thanks! From Marilyn.A.Weiss <@t> kp.org Fri Sep 2 18:02:02 2011 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri Sep 2 18:03:10 2011 Subject: [Histonet] out of office Message-ID: I will be out of the office starting 09/02/2011 and will not return until 09/06/2011. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. From shirin_ake <@t> yahoo.com Mon Sep 5 03:13:06 2011 From: shirin_ake <@t> yahoo.com (shirin kouhpayeh) Date: Mon Sep 5 03:13:09 2011 Subject: [Histonet] (no subject) Message-ID: <1315210386.18596.YahooMailMobile@web161005.mail.bf1.yahoo.com> Dear histonetters I'm working on a surface marker, uPAR, on lymph node tissue. For setting up the IHC-p procedure, I've chosen the ductal carcinoma sample as positive control. I use the citrate buffer ph=6 as retrieval solution and TBS ph=7.6 for wash steps. different Ag retrieval systems and different Ab ( santa cruz biotech. ) dilutions have been tested s 2*30 minutes of heating in water bath 70 degree of cellicius, 5 minutes with 800 watt power in microwave oven following 15 minutes heating with power of 300 6 minutes with max power ( 800 watt) following by 15 minutes on power 300 watt 10 minutes with max power following with 15 minutes on power 300 watt 20 min autoclave on 121 degree of cellicius But there is still no signal on ductal carcinoma sample. Is there anyone have a comment for me? I would be so appreciated . Shirin kouhpayeh Ph.D student Immunology dept. Isfahan university of medical science Isfahan, Iran From daiyan1828 <@t> yahoo.com.cn Mon Sep 5 09:11:43 2011 From: daiyan1828 <@t> yahoo.com.cn (=?gb2312?B?zuK66NHj?=) Date: Mon Sep 5 09:11:54 2011 Subject: [Histonet] ihc antibody Message-ID: <1315231903.54988.YahooMailClassic@web15703.mail.cnb.yahoo.com> which antibody did well in xp11.3 renal cancer?From joost.bruijntjes <@t> tno.triskelion.nl Tue Sep 6 04:54:57 2011 From: joost.bruijntjes <@t> tno.triskelion.nl (Bruijntjes, J.P. (Joost)) Date: Tue Sep 6 04:55:08 2011 Subject: [Histonet] (no subject) Message-ID: Hi all I have to prepare frozen slides of zebra fish, but it is not permitted to use OCT because of the presence of different polymers in the OCT compound. Is anyone of you familiar with the preparation of frozen slides without using OCT? Thanks Joost Bruijntjes Zeist Holland TNO.NL Joost Bruijntjes T +31 88 866 17 38 F +31 30 694 49 86 E joost.bruijntjes@tno.triskelion.nl Disclaimer From trathborne <@t> somerset-healthcare.com Tue Sep 6 07:59:49 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Sep 6 08:00:05 2011 Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) In-Reply-To: <2342C8D836A0954199F6D58A44BDD1BE0DD9FD@XCHMB01.allina.com> References: <1314840234.28912.YahooMailClassic@web39404.mail.mud.yahoo.com>, <493CAA64F203E14E8823737B9EE0E25F0900642E81@EXCHMB01.isis.local> <2342C8D836A0954199F6D58A44BDD1BE0DD9FD@XCHMB01.allina.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F553FF@SMCMAIL01.somerset-healthcare.com> We use the coverglass from StatLab - no problems. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fleming, Jackie M Sent: Thursday, September 01, 2011 4:41 PM To: Bea DeBrosse-Serra; 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) Must use the Leica coverslips - OR it's a lot of downtime! Stainer is slower than we had hoped, but we have a really high volume. Great service! Jackie Fleming HT ASCP Allina Medical Laboratries Histology Technical Consultant phone: 612-863-4773 pager: 612-654-2135 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bea DeBrosse-Serra [BDeBrosse-Serra@isisph.com] Sent: Thursday, September 01, 2011 3:38 PM To: 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic H&E stainer with coverslipper (glass) Leica.......all the way! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination H&E stainer with glass coverslipper. Thank you. Ernestine Middleton, Manager, HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emiddlet@montefiore.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From brett_connolly <@t> merck.com Tue Sep 6 10:20:14 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Sep 6 10:20:22 2011 Subject: [Histonet] Hexokinase 1 (HK1) antibody for IHC? Message-ID: Hello all, Can anybody recommend a good polyclonal anti-HK1 antibody for FFPE sections. We want to avoid using mouse monoclonals. Thanks much, Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From rjbuesa <@t> yahoo.com Tue Sep 6 11:22:13 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 6 11:22:17 2011 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <1315326133.90648.YahooMailClassic@web65705.mail.ac4.yahoo.com> That will depend on the size of the fish. If less than 5 mm you could use what I used doing DIF of skin cases: Place the chuck on the freezing bar and add?2-3 drops of distilled water. Introduce the fish inside the drop of water close to the chuck surface, and add another drop. Using the coolant aerosol make sure that the drop is completely frozen. Proceed to cut. Ren? J. --- On Tue, 9/6/11, Bruijntjes, J.P. (Joost) wrote: From: Bruijntjes, J.P. (Joost) Subject: [Histonet] (no subject) To: "Histonet@lists.utsouthwestern.edu" Date: Tuesday, September 6, 2011, 5:54 AM Hi all I have to prepare frozen slides of zebra fish, but it is not permitted to use OCT because of the presence of different polymers in the OCT compound. Is anyone of you familiar with the preparation of frozen slides without using OCT? Thanks Joost Bruijntjes Zeist Holland TNO.NL Joost Bruijntjes T +31 88 866 17 38 F +31 30 694 49 86 E joost.bruijntjes@tno.triskelion.nl Disclaimer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGleiberman <@t> cbiolabs.com Tue Sep 6 11:33:26 2011 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Tue Sep 6 11:33:32 2011 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C0B5869D4@cbiolabs05.CBiolabs.local> You can freeze samples (either fresh or formalin fixed, but in later case with sucrose cryoprotection) in 7% gelatin in PBS instead of OCT. Sometimes it gives much better conditions for cutting as well. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruijntjes, J.P. (Joost) Sent: Tuesday, September 06, 2011 5:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi all I have to prepare frozen slides of zebra fish, but it is not permitted to use OCT because of the presence of different polymers in the OCT compound. Is anyone of you familiar with the preparation of frozen slides without using OCT? Thanks Joost Bruijntjes Zeist Holland TNO.NL Joost Bruijntjes T +31 88 866 17 38 F +31 30 694 49 86 E joost.bruijntjes@tno.triskelion.nl Disclaimer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From gu.lang <@t> gmx.at Tue Sep 6 11:48:47 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Sep 6 11:48:52 2011 Subject: [Histonet] copy of article Message-ID: <069CCD61BA834B3B93BECE3239FB635B@dielangs.at> Dear histonetters, Is it possible to get a copy of following article? Horobin RW, Staining by numbers: a tool for understanding and assisting use of routine and special histopathology stains, J Histotechnol 2004; 27: 23-28. I would highly appreciate your help. Regards Gudrun Lang Histolab, Linz Austria From b427297 <@t> aol.com Tue Sep 6 11:50:45 2011 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Tue Sep 6 11:51:04 2011 Subject: [Histonet] Re:howdy, Message-ID: <8CE3ACC41771545-660-58266@webmail-d078.sysops.aol.com> .. http://mediofactor.it/index59--.php?tocampID=+7j7 From Ken_Marissael <@t> vwr.com Tue Sep 6 13:39:24 2011 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Tue Sep 6 13:39:31 2011 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 09/06/2011 and will not return until 09/12/2011. I will be away on vacation from 9/6 and return 9/12. While I am away, please contact VWR Healthcare Customer Service at 877-881-1192. If you have an urgent requirement, please contact either Jackie Zerillo (609)410-6152 or Scott Nuetzel (410)302-3045. From gu.lang <@t> gmx.at Tue Sep 6 13:43:54 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Sep 6 13:44:00 2011 Subject: AW: [Histonet] copy of article In-Reply-To: <069CCD61BA834B3B93BECE3239FB635B@dielangs.at> References: <069CCD61BA834B3B93BECE3239FB635B@dielangs.at> Message-ID: Thank you histonetters for your big help. I've got the article sent. Let's go reading... Regards Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Dienstag, 06. September 2011 18:49 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] copy of article Dear histonetters, Is it possible to get a copy of following article? Horobin RW, Staining by numbers: a tool for understanding and assisting use of routine and special histopathology stains, J Histotechnol 2004; 27: 23-28. I would highly appreciate your help. Regards Gudrun Lang Histolab, Linz Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NLEMKE1 <@t> hfhs.org Tue Sep 6 14:06:36 2011 From: NLEMKE1 <@t> hfhs.org (Lemke, Nancy) Date: Tue Sep 6 14:06:45 2011 Subject: [Histonet] molecular fixatives Message-ID: <66EF8D68E063C044B9AC46E99E3502BF03D3876B@RHWMBX01.corp.ds.hfhs.org> I am gathering information on molecular fixatives, specifically one from Theranostics Health and one From Sakura, developed for the XPress processors. Does anyone have experience with either or both of these for RNA, DNA and protein work as well as IHC for phospho antibodies? I would really appreciate some feedback. Thanks, Nancy W Lemke Research Coordinator Hermelin Brain Tumor Center rm 3115 E&R Bldg Neurosurgery Research Henry Ford Hospital 2799 W Grand Blvd Detroit, MI 48202 ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From rboen <@t> clearwire.net Tue Sep 6 14:23:59 2011 From: rboen <@t> clearwire.net (Rick and Sue Boen) Date: Tue Sep 6 14:24:10 2011 Subject: [Histonet] Decal Solution for Bone Marrow Cores Message-ID: We're in the process of switching over to the Bond III. For users of Leica's Bond, what is your favorite decal solution to be used for ISH kappa and lambda? Thanks in advance. Rick Boen BS, HTL(ASCP) St.Luke's Hospital Duluth, MN From SohrabB1 <@t> ah.org Tue Sep 6 14:38:36 2011 From: SohrabB1 <@t> ah.org (Behnaz Sohrab) Date: Tue Sep 6 14:38:43 2011 Subject: [Histonet] Recycler Message-ID: <4E66144C.4347.0054.1@ah.org> We are in the market to buy recycling system, any recommendation between CBG or B/R system ?? Any suggestions is deeply appreciated. Thanks Behnaz Sohrab,Manager Anatomic Pathology From kgrobert <@t> rci.rutgers.edu Tue Sep 6 14:44:18 2011 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Tue Sep 6 14:44:21 2011 Subject: [Histonet] Leica CV5030 and Autostainer XL Message-ID: To all, We got these at auction, and I am currently figuring out a) where to put them and b) how they go together, and of course there isn't a whole lot of information in the user manuals about setting these things up. I know, we should go to Leica for help, but my boss wants me to try it first. Would anybody in NJ happen to have these machines, and if so, may I come take a look at them? Thanks so much, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 From KCross <@t> cvm.tamu.edu Tue Sep 6 15:02:59 2011 From: KCross <@t> cvm.tamu.edu (Kelly Cross) Date: Tue Sep 6 15:03:06 2011 Subject: [Histonet] Recycler In-Reply-To: <4E66144C.4347.0054.1@ah.org> References: <4E66144C.4347.0054.1@ah.org> Message-ID: <4E663623020000E3000DA1C1@CVM.TAMU.EDU> CBG all the way! Efficient! Simple! Essentially problem free in my lab! Kelly Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Texas A&M University Veterinary Pathobiology VMA bldg. 207B College Station, TX 77843-4467 Office: 979-862-3658 Lab: 979-845-5149 Fax: 979-862-1147 kcross@cvm.tamu.edu >>> "Behnaz Sohrab" 9/6/2011 2:38 PM >>> We are in the market to buy recycling system, any recommendation between CBG or B/R system ?? Any suggestions is deeply appreciated. Thanks Behnaz Sohrab,Manager Anatomic Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nacaela.Johnson <@t> USONCOLOGY.COM Tue Sep 6 15:11:51 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Tue Sep 6 15:12:54 2011 Subject: [Histonet] Decal Solution for Bone Marrow Cores In-Reply-To: References: Message-ID: <71882EED22A283429E8424513A22922D5953E5@txhous1eb015.uson.usoncology.int> BBC Rapid Cal Immuno. It's the only one that I could use so that ISH could work. There is still quite a bit of background on the ISH that I have not overcome, but at least the staining is there. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rick and Sue Boen Sent: Tuesday, September 06, 2011 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal Solution for Bone Marrow Cores We're in the process of switching over to the Bond III. For users of Leica's Bond, what is your favorite decal solution to be used for ISH kappa and lambda? Thanks in advance. Rick Boen BS, HTL(ASCP) St.Luke's Hospital Duluth, MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From rjbuesa <@t> yahoo.com Tue Sep 6 15:29:58 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 6 15:30:01 2011 Subject: [Histonet] Recycler In-Reply-To: <4E66144C.4347.0054.1@ah.org> Message-ID: <1315340998.57920.YahooMailClassic@web65711.mail.ac4.yahoo.com> I would buy (again) the B/R system. I used to recycle xylene and the investment was repaid in3 years. No problems with the instrument if you service it (yourself, no need to call outside help). Ren? J --- On Tue, 9/6/11, Behnaz Sohrab wrote: From: Behnaz Sohrab Subject: [Histonet] Recycler To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 6, 2011, 3:38 PM We are in the market to buy recycling system, any recommendation between CBG or B/R system ??? Any suggestions is deeply appreciated. Thanks Behnaz Sohrab,Manager Anatomic Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sadey <@t> hotmail.ca Tue Sep 6 18:22:03 2011 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Tue Sep 6 18:22:08 2011 Subject: [Histonet] Any Picis users??? Message-ID: Hello netters, Is anyone using a surgical pathology system called Picis??? Our OR is considering this system and we are wondering if it would benefit the pathology side of things. Any input is appreciated. Thanks :) Sheila From histotalk <@t> yahoo.com Tue Sep 6 20:45:48 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Tue Sep 6 20:45:51 2011 Subject: [Histonet] HistoTALK at the NSH Convention Message-ID: <1315359948.32214.YahooMailNeo@web120618.mail.ne1.yahoo.com> Hello again HistoNetters - ? As you know HistoTALK will be taping interviews for its Sunday shows this year at the NSH Convention in Cincinnati. I am scheduling interview times now through Wednesday, September 14th. Here are the days and times available at the HistoTALK "mobile studio" - ? Friday, September 16th after 6:30 pm (maybe) Saturday, September 17th from 8 am to 1 pm. Sunday, September 18th from 11 am to 4 pm. and Wednesday, September 21st from 1 pm to 4 pm. ? You will find the HistoTALK "studio", OK it's a table,?in the Convention Center lobby. ? Let me know if you are interested, the day and time?and I'll send you the details sheet. If you have been on before and would like to be on again, not a problem. ? Remember, your interview is NOT LIVE! It's taped for future broadcast. ? Yours, Dave www.DavidKemler.info ? P.S. If you emailed me about doing an interview and you haven't heard back from me - email me again. Things have a tendency?of?getting lost in my office. From tmalloy77 <@t> hotmail.com Wed Sep 7 04:44:54 2011 From: tmalloy77 <@t> hotmail.com (TIMOTHY MALLOY) Date: Wed Sep 7 04:44:59 2011 Subject: [Histonet] Re:2 Message-ID: ..Yeah! It?s really cool! This site is everything you need! http://niftytrends.megabyet.net/friends.page.php?afriend_id=37og9 From christiegowan <@t> msn.com Wed Sep 7 06:09:35 2011 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Wed Sep 7 06:09:37 2011 Subject: [Histonet] Re:2 In-Reply-To: References: Message-ID: Sorry to all my histonet friends. Someone hacked my email account and is sending out all these lovely little smam monsters. I have since addressed the issue and I hope it has stopped. Please let me know if you are still getting the little gifts and I will have to take more drastic measures. > From: tmalloy77@hotmail.com > To: ejmalloy@gmail.com; jf73@rocketmail.com; eric@bestpiti.com; xdgentile@sbcglobal.net; histonet@lists.utsouthwestern.edu > Date: Wed, 7 Sep 2011 05:44:54 -0400 > CC: > Subject: [Histonet] Re:2 > > ..Yeah! It?s really cool! This site is everything you need! http://niftytrends.megabyet.net/friends.page.php?afriend_id=37og9 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JThawley <@t> ShoreMemorial.org Wed Sep 7 06:24:48 2011 From: JThawley <@t> ShoreMemorial.org (JThawley@ShoreMemorial.org) Date: Wed Sep 7 06:24:54 2011 Subject: [Histonet] Leica CV5030 and Autostainer XL In-Reply-To: Message-ID: Hey Kathleen, We actually have both the CV5030 and Autostainer XL if you want to come check them out you are welcome to it. We are located by Ocean City, NJ. Call me or email me. Jennifer Thawley HT, ASCP Histology Supervisor Shore Memorial Hospital (609) 653-3940 kgrobert@rci.rutg ers.edu Sent by: To histonet-bounces@ "histonet" lists.utsouthwest ern.edu cc Subject 09/06/2011 03:44 [Histonet] Leica CV5030 and PM Autostainer XL To all, We got these at auction, and I am currently figuring out a) where to put them and b) how they go together, and of course there isn't a whole lot of information in the user manuals about setting these things up. I know, we should go to Leica for help, but my boss wants me to try it first. Would anybody in NJ happen to have these machines, and if so, may I come take a look at them? Thanks so much, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sylvia.Hewell <@t> propath.com Wed Sep 7 07:35:13 2011 From: Sylvia.Hewell <@t> propath.com (Sylvia Hewell) Date: Wed Sep 7 07:34:56 2011 Subject: [Histonet] (no subject) Message-ID: <82C7248978CB50469FD6BA68EBBEFE6706394C0C@exchange.propathlab.com> Sylvia Hewell, HT (ASCP) Histology Supervisor ProPath Lewisviile Medical Center 500 W. Main St. 972-420-1552 or 972-420-1887 To learn more about ProPath, please visit http://www.ProPath.com Sylvia.Hewell@propath.com The information contained in this message may be privileged and confidential. If you are NOT the intended recipient, please notify the sender immediately with a copy to Postmaster@propath.com and destroy this message. From mhale <@t> carisls.com Wed Sep 7 09:14:27 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Wed Sep 7 09:15:56 2011 Subject: [Histonet] New Mexico Grossing Message-ID: <6F33D8418806044682A391273399860F09997FBA@s-irv-ex301.PathologyPartners.intranet> Is anyone aware of the grossing regulations in New Mexico for biopsies ?Thxs Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From tmalloy77 <@t> hotmail.com Wed Sep 7 10:49:12 2011 From: tmalloy77 <@t> hotmail.com (TIMOTHY MALLOY) Date: Wed Sep 7 10:49:19 2011 Subject: [Histonet] Re:We can help you with impotence! Message-ID: ...OMG! It?s a sexual revolution! I?ve never thought it possible! http://lajoiedeparler.net/com.friend.php?noghotmailID=56j8 From SteveM <@t> mcclainlab.com Wed Sep 7 13:17:29 2011 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Wed Sep 7 13:04:52 2011 Subject: [Histonet] Recycler (Behnaz Sohrab) xylene test for recycled alcohols product or alcohols to be recycled Message-ID: 7 years with the same B/R Procycler Would definitely buy again. It has paid for itself, even in a small lab. Good company. They will analyze your product and suggest revisions in the recycling programs as needed. How you use it and what you put into it to distill generally determines your results. Excellent for xylene 70-90% recovery (3.5-4.5 gallons from 5 gallon run). Reagent Alcohols we only recycle 90-100% with No contact with xylene and get back 95% suitable for processor- we do not use recycled alcohols for staining. We have not been brave or foolish enough to try it again after some issues early on. We do not use isopropyl alcohol. You can program to recycle lower concentrations of alcohol, BUT 1) it takes longer to run and 2) our product results early on were not as consistent. Keep it clean. We do not recycle alcohol purges from processors nor the two absolutes after xylene on the stainers. If you add any contaminated alcohols, you contaminate the whole 5 gallons. We learned to do the "xylene test"- when you suspect an alcohol is contaminated with xylene. Add equal volumes of DH2O and suspect reagent in a 100ml graduated cylinder or long neck volumetric flask. Let stand for 5 minutes, the xylene will appear on top. Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 From pathologylab <@t> ymail.com Wed Sep 7 13:54:34 2011 From: pathologylab <@t> ymail.com (Pathology Lab) Date: Wed Sep 7 13:54:38 2011 Subject: [Histonet] Looking for an use Shandon Varistain Slide Stainer Message-ID: <1315421674.6658.YahooMailNeo@web121414.mail.ne1.yahoo.com> We are looking for an used Shandon Varistain Slide Stainer ? Lcda. Mary V. Guerrero,BS, MBA,HtL ? ? ? ? ? ? ? Administradora/Coordinadora General ?Pathology Lab.? ? 55?N. Dr. Basora Edificio M?dico IV?Oficina 206 Mayaguez, Puerto Rico 00680 Tel. 787-834-8202 ?Fax: 787-831-5255 Sra. Dimary Valent?n?????????? Sra. Iris Franqui??????????????? Sra. Myrna Gonz?lez ? Facturaci?n???????? Reportes???????? ? ? Transcripcion This email may contain confidential health information that is legally privileged.? This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled.? If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited.? If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . From mhardin <@t> carisls.com Wed Sep 7 14:10:51 2011 From: mhardin <@t> carisls.com (Hardin, Michelle) Date: Wed Sep 7 14:10:55 2011 Subject: [Histonet] Caris Life Sciences is looking for an experienced Histotech Message-ID: We are seeking an experience Histotech for our lab in Phoenix, AZ. We are on the cutting edge in the biotechnology industry and are looking for dynamic lab professionals to join our team. If you would be interested, please contact me for details! From tkngflght <@t> yahoo.com Wed Sep 7 14:56:48 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Sep 7 14:56:54 2011 Subject: [Histonet] New Mexico and CLIA Message-ID: <1315425408.23316.YahooMailNeo@web39407.mail.mud.yahoo.com> http://dhi.health.state.nm.us/CLIA/index.php ? http://dhi.health.state.nm.us/CLIA/clia_requirements.php Basically reinforces the conformation/confirmation?that providers for NM are adhering to the broader CLIA regulations regarding healthcare delivery.? The only major exceptions are DOD facilities located in the state. ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From jclark <@t> pcnm.com Wed Sep 7 15:33:52 2011 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Sep 7 15:33:54 2011 Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 7 In-Reply-To: <20110907170527.0878C63E88F@mx10.myoutlookonline.com> References: <20110907170527.0878C63E88F@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E3784CA7DA40@S10MAILD001N2.SH10.lan> Hi Meredith, as far as I know New Mexico follows the CAP guidelines (CLIA88 regs) for who can gross what. I am an HT with an associates and I do the grossing of both biopsies and bigger more complicated specimens. You need an exact list of what a 'non pathologist' is approved to gross in your facility. Documentation of training and how the grossing is reviewed by the lab director is also required. According to CLIA88 you need to have an associates degree to do grossing of either biopsies or bigger specimens. All grossing is now considered high complexity testing. We just had our CAP inspection and were fine with how we have it set up and documented for a non pathologist to gross. You're the new GI lab in Las Cruces correct? Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Roswell, NM ------------------------------ Message: 16 Date: Wed, 7 Sep 2011 09:14:27 -0500 From: "Hale, Meredith" Subject: [Histonet] New Mexico Grossing To: Message-ID: <6F33D8418806044682A391273399860F09997FBA@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Is anyone aware of the grossing regulations in New Mexico for biopsies ?Thxs Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From amosbrooks <@t> gmail.com Wed Sep 7 17:47:54 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Sep 7 17:48:01 2011 Subject: [Histonet] Digital Security Message-ID: Hi, When this happens, first change your email password. That may be the best way to prevent further breaches. Next scan your PC (certainly not a Mac or linux right?) with a good antivirus program. AVG and Clamwin are both decent free antivirus programs. Next it would be good to scan for things that are not really viruses, but are still malware. The best program I found for this is Spybot Search & Destroy. All these can be found on CNet's software website www.download.com I know this isn't really lab related, but I did send it to the whole list since there seems to be a lot of folks that this seems to be happening to. Digital security is always relevant. Good Bug Hunting, Amos On Wed, Sep 7, 2011 at 1:00 PM, wrote: > Message: 13 > Date: Wed, 7 Sep 2011 11:09:35 +0000 > From: CHRISTIE GOWAN > Subject: RE: [Histonet] Re:2 > To: , , > , , < > xdgentile@sbcglobal.net>, > > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > > Sorry to all my histonet friends. Someone hacked my email account and is > sending out all these lovely little smam monsters. I have since addressed > the issue and I hope it has stopped. Please let me know if you are still > getting the little gifts and I will have to take more drastic measures. > > > > From: tmalloy77@hotmail.com > > To: ejmalloy@gmail.com; jf73@rocketmail.com; eric@bestpiti.com; > xdgentile@sbcglobal.net; histonet@lists.utsouthwestern.edu > > Date: Wed, 7 Sep 2011 05:44:54 -0400 > > CC: > > Subject: [Histonet] Re:2 > > > > ..Yeah! It?s really cool! This site is everything you need! > http://niftytrends.megabyet.net/friends.page.php?afriend_id=37og9 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shrivastavaanuradha <@t> hotmail.com Wed Sep 7 20:11:12 2011 From: shrivastavaanuradha <@t> hotmail.com (anuradha shrivastava) Date: Wed Sep 7 20:11:33 2011 Subject: [Histonet] CAP general checklist for expiry on the chemicals. Message-ID: Hello Everybody, need yr advice on 95%,70%and 50% alcohol which r made in house from 100%. How to put an expiration date on them. I will appreciate your help. Thanks. Anu. From member <@t> linkedin.com Wed Sep 7 21:17:42 2011 From: member <@t> linkedin.com (Melinda Hamilton via LinkedIn) Date: Wed Sep 7 21:17:44 2011 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <839829949.3931931.1315448262333.JavaMail.app@ela4-app0128.prod> LinkedIn ------------ Melinda Hamilton requested to add you as a connection on LinkedIn: ------------------------------------------ David, I'd like to add you to my professional network on LinkedIn. - Melinda Accept invitation from Melinda Hamilton http://www.linkedin.com/e/yvpgd1-gsb3xj95-2c/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I156744395_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYRejcQd3sSdj59bPsSu6oNj59AbP8RcjANd3oOcjgLrCBxbOYWrSlI/EML_comm_afe/?hs=false&tok=2pHEeZg4sFykU1 View invitation from Melinda Hamilton http://www.linkedin.com/e/yvpgd1-gsb3xj95-2c/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I156744395_13/3cNnPkVcPgQdPoRckALqnpPbOYWrSlI/svi/?hs=false&tok=1j0x2KAlwFykU1 ------------------------------------------ Why might connecting with Melinda Hamilton be a good idea? Melinda Hamilton's connections could be useful to you: After accepting Melinda Hamilton's invitation, check Melinda Hamilton's connections to see who else you may know and who you might want an introduction to. Building these connections can create opportunities in the future. -- (c) 2011, LinkedIn Corporation From sbaldwin <@t> mhhcc.org Thu Sep 8 08:08:52 2011 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Thu Sep 8 08:09:01 2011 Subject: [Histonet] P63 controls Message-ID: Hi Histonetters We are having trouble with our control for P63 what is everyone using? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. From sbreeden <@t> nmda.nmsu.edu Thu Sep 8 08:09:09 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Sep 8 08:09:17 2011 Subject: [Histonet] Nuclear Fast Red Substitute? Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFA82@nmdamailsvr.nmda.ad.nmsu.edu> What can I use as a substitute for NFR for Prussian Blue iron stain, please? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From Ronald.Houston <@t> nationwidechildrens.org Thu Sep 8 08:21:42 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Sep 8 08:21:55 2011 Subject: [Histonet] RE: Nuclear Fast Red Substitute? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DFA82@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B051DFA82@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: 1% aq neutral red (CI 50040) Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, September 08, 2011 9:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear Fast Red Substitute? What can I use as a substitute for NFR for Prussian Blue iron stain, please? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From LSebree <@t> uwhealth.org Thu Sep 8 08:32:02 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Sep 8 08:32:31 2011 Subject: [Histonet] P63 controls In-Reply-To: References: Message-ID: Normal breast -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Thursday, September 08, 2011 8:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P63 controls Hi Histonetters We are having trouble with our control for P63 what is everyone using? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Kay <@t> albertahealthservices.ca Thu Sep 8 09:14:57 2011 From: Karen.Kay <@t> albertahealthservices.ca (Karen Kay) Date: Thu Sep 8 09:15:19 2011 Subject: [Histonet] RECYCLERS - RE: Histonet Digest, Vol 94, Issue 7 In-Reply-To: <20110907170242.ACAB420F07A@scutum.calgaryhealthregion.ca> References: <20110907170242.ACAB420F07A@scutum.calgaryhealthregion.ca> Message-ID: <14E77BDEC6B22749B75CAC82EC2090FF03D9A0EAF2@EXMBXC5.crha.bewell.ca> Good Morning, We use the CBG recycler and are very happy with both the performance of the instrument and support provided by the company. Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital South Zone West - Alberta Health Services Lethbridge, Alberta,CANADA karen.kay@albertahealthservices.ca Message: 5 Date: Tue, 06 Sep 2011 12:38:36 -0700 From: "Behnaz Sohrab" Subject: [Histonet] Recycler To: Message-ID: <4E66144C.4347.0054.1@ah.org> Content-Type: text/plain; charset=US-ASCII We are in the market to buy recycling system, any recommendation between CBG or B/R system ?? Any suggestions is deeply appreciated. Thanks Behnaz Sohrab,Manager Anatomic Pathology This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From alyssa <@t> alliedsearchpartners.com Thu Sep 8 11:03:56 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Sep 8 11:04:01 2011 Subject: [Histonet] Histology Position in WA State Message-ID: Allied Search Partners is currently looking for a Histology Professional to work in Washington State. Please email me your resume and/or questions if interested in this position. Position Title: Histotech Schedule: Full Time/Permanent Summary and Requirements: 1 year+ experience with all aspects of Histology ASCP Preferred Benefits: Medical / Dental / Optical Insurance, Life Insurance, 401K Retirement, EAP Program, Tuition Reimbursement, Paid Holiday and PTO, Continuing Education, Personal Insurance To Apply: Please send resume to alyssa@alliedsearchpartners.com. If all qualifications are met then we will call to schedule a phone screen with one of our recruiters. -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with ?Remove.? * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From Ashley.Troutman <@t> Vanderbilt.Edu Thu Sep 8 14:16:21 2011 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Thu Sep 8 14:16:32 2011 Subject: [Histonet] P63 controls Message-ID: <7B310892042DA74CB3590053F424CFE61441FCEE1C@ITS-HCWNEM06.ds.Vanderbilt.edu> We use normal prostate and we have also used skin in the past-they both work well. I would avoid pre-cutting a lot of controls, though; it has a tendency to loose antigenicity. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 9 Date: Thu, 8 Sep 2011 09:08:52 -0400 From: "Sara Baldwin/mhhcc.org" Subject: [Histonet] P63 controls To: Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters We are having trouble with our control for P63 what is everyone using? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. From Marcia_Gaiser <@t> ssmhc.com Thu Sep 8 15:16:48 2011 From: Marcia_Gaiser <@t> ssmhc.com (Gaiser, Marcia) Date: Thu Sep 8 15:16:56 2011 Subject: [Histonet] Full Time HT position in Oklahoma City Message-ID: <728F817C02110E498D803A7C3B0C62480690A37B6E@S009-APEXM06.ds.ad.ssmhc.com> POSITION REQUIREMENTS: EDUCATION: High school diploma or GED preferred. CERTIFICATION, LICENSURE, BONDING: Certified as an HT or HLT by the American Society of Clinical Pathologists (ASCP) ? or ? other nationally recognized certifying agency acceptable to the Laboratory Director ? or ? experience acceptable to the Laboratory Director. EXPERIENCE: Two years of satisfactory histology experience. SIGN ON BONUS AVAILABLE FOR QUALIFIED CANDIDATES. Outstanding benefits package including generous paid time off. Apply online at www.saintsok.com>, Ad# 10762, or contact Christina Hughes at (405) 272-6105 for more information. Thank you! Christina Hughes HR Recruiter St. Anthony Hospital (405) 272-6105 - phone (405) 272-6781 - fax Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ From pathlocums <@t> gmail.com Thu Sep 8 15:37:12 2011 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Thu Sep 8 15:37:15 2011 Subject: [Histonet] STP420 vs. Peloris Message-ID: Considering both options. Can any STP users out there give me opinions? Please give reasons, not just "go with" this one. Thanks. From charleso.o606 <@t> gmail.com Thu Sep 8 17:07:35 2011 From: charleso.o606 <@t> gmail.com (Charles O) Date: Thu Sep 8 17:07:39 2011 Subject: [Histonet] Seeking new position Message-ID: Hi, I am a certified histotech seeking a new position. I have an extensive experience in the general histological technics and in Immunohistochemistry. I am willing to relocate. Thanks, Charles O. From shrivastavaanuradha <@t> hotmail.com Thu Sep 8 19:55:08 2011 From: shrivastavaanuradha <@t> hotmail.com (anuradha shrivastava) Date: Thu Sep 8 19:55:27 2011 Subject: [Histonet] Comment on HT/HTL Message-ID: Hi All, I feel so fortunate that I studied Histo in Cobleskill, with my bio background It was perfect. I think the science sub. background is also needed specially anatomy, so that one knows how to embed different tissues. I hope u all will agree with me. Slide submittion should be there in the exam. Anu Shrivastava. M.S , HTL (ASCP) Histology Team Leader, South County Hospital RI From nicole <@t> dlcjax.com Fri Sep 9 08:21:06 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Fri Sep 9 08:23:39 2011 Subject: [Histonet] Facebook Message-ID: <4934.208.62.167.196.1315574466.squirrel@webmail.realpages.com> Hey histonetters, Ive created a community page for histologist on Facebook. Please join or like Histo-ville. It a page for people to make friends, troubleshoot, or discuss issues. I hope to create a large community of friends. So stop by and say hi. I look forward to meeting fellow techs. Nicole Tatum, HT ASCP From tahseen <@t> brain.net.pk Fri Sep 9 08:34:25 2011 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Fri Sep 9 08:34:33 2011 Subject: [Histonet] thinprep Message-ID: <62815.203.135.35.66.1315575265.squirrel@brain.net.pk> We want to purchase a latest model machine for thin prep/sure path along with an auto stainer for pap.stain. Can you share your experience regarding both types of machines, 1) Which type of system is best 2)which machine will provide an auto stainer 3)which model of the recommended machine is best 4)will they arrange for training of cytotechnologist and pathologist for processing and reportting of specimen 5)which company is providing best after sale service Thanks From JWeems <@t> sjha.org Fri Sep 9 09:33:42 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Sep 9 09:33:48 2011 Subject: [Histonet] I'm so sad for AFIP to close Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8BBEB@CHEXCMS10.one.ads.che.org> I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From sdysart <@t> mirnarx.com Fri Sep 9 10:02:43 2011 From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com) Date: Fri Sep 9 10:02:50 2011 Subject: [Histonet] I'm so sad for AFIP to close In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8BBEB@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8BBEB@CHEXCMS10.one.ads.che.org> Message-ID: Wow...that is sad news. Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat hology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Sep 9 10:07:32 2011 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Fri Sep 9 10:07:49 2011 Subject: [Histonet] I'm so sad for AFIP to close In-Reply-To: References: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8BBEB@CHEXCMS10.one.ads.che.org> Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4EC22F6DF@EXC-MBX3.cfs.le.ac.uk> Well unfortunately one can't have public/governmental spending cut backs without losing something important along the way. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sdysart@mirnarx.com Sent: 09 September 2011 16:03 To: JWeems@sjha.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] I'm so sad for AFIP to close Wow...that is sad news. Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat hology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HMLaudon <@t> gundluth.org Fri Sep 9 10:23:16 2011 From: HMLaudon <@t> gundluth.org (Laudon, Heather M) Date: Fri Sep 9 10:23:20 2011 Subject: [Histonet] Decal for IHC Message-ID: Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 From POWELL_SA <@t> mercer.edu Fri Sep 9 10:32:47 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Sep 9 10:32:51 2011 Subject: [Histonet] RE: I'm so sad for AFIP to close In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8BBEB@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8BBEB@CHEXCMS10.one.ads.che.org> Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE9967AE@MERCERMAIL.MercerU.local> I know, just breaks your heart. I spent 3 weeks there in 19...... well a long time ago. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Fri Sep 9 10:40:48 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 9 10:40:52 2011 Subject: [Histonet] Floater Problem Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2B0BA@EXCHANGE3.huntingtonhospital.com> I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert From rjbuesa <@t> yahoo.com Fri Sep 9 10:45:54 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 9 10:45:57 2011 Subject: [Histonet] Decal for IHC In-Reply-To: Message-ID: <1315583154.12504.YahooMailClassic@web65711.mail.ac4.yahoo.com> EDTA pH 7 Ren? J. --- On Fri, 9/9/11, Laudon, Heather M wrote: From: Laudon, Heather M Subject: [Histonet] Decal for IHC To: "histonet@lists.utsouthwestern.edu" Date: Friday, September 9, 2011, 11:23 AM Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Sep 9 10:45:08 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Fri Sep 9 10:46:46 2011 Subject: [Histonet] RE: Floater Problem In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2B0BA@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D76830AD2B0BA@EXCHANGE3.huntingtonhospital.com> Message-ID: I have also heard of the occasional floater coming from the processor and making its way into the paraffin block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, September 09, 2011 11:41 AM To: histonet@lists.utsouthwestern.edu Cc: Debra Cobb Subject: [Histonet] Floater Problem I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KCross <@t> cvm.tamu.edu Fri Sep 9 10:49:29 2011 From: KCross <@t> cvm.tamu.edu (Kelly Cross) Date: Fri Sep 9 10:49:43 2011 Subject: [Histonet] RE: I'm so sad for AFIP to close In-Reply-To: <9BF995BC0E47744E9673A41486E24EE238DE9967AE@MERCERMAIL.MercerU.local> References: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8BBEB@CHEXCMS10.one.ads.che.org> <9BF995BC0E47744E9673A41486E24EE238DE9967AE@MERCERMAIL.MercerU.local> Message-ID: <4E69EF39020000E3000DAE6A@CVM.TAMU.EDU> As a young histotech I felt I had "arrived" when my supervisor assigned me to take care of the AFIP study set years ago! It is sad... Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Texas A&M University Veterinary Pathobiology VMA bldg. 207B College Station, TX 77843-4467 Office: 979-862-3658 Lab: 979-845-5149 Fax: 979-862-1147 kcross@cvm.tamu.edu >>> "Shirley A. Powell" 9/9/2011 10:32 AM >>> I know, just breaks your heart. I spent 3 weeks there in 19...... well a long time ago. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KCross <@t> cvm.tamu.edu Fri Sep 9 10:50:32 2011 From: KCross <@t> cvm.tamu.edu (Kelly Cross) Date: Fri Sep 9 10:50:44 2011 Subject: [Histonet] Floater Problem In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2B0BA@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D76830AD2B0BA@EXCHANGE3.huntingtonhospital.com> Message-ID: <4E69EF78020000E3000DAE71@CVM.TAMU.EDU> Water bath???? Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Texas A&M University Veterinary Pathobiology VMA bldg. 207B College Station, TX 77843-4467 Office: 979-862-3658 Lab: 979-845-5149 Fax: 979-862-1147 kcross@cvm.tamu.edu >>> "Laurie Colbert" 9/9/2011 10:40 AM >>> I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Fri Sep 9 10:52:52 2011 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Fri Sep 9 10:54:18 2011 Subject: [Histonet] RE: Floater Problem In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2B0BA@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D76830AD2B0BA@EXCHANGE3.huntingtonhospital.com> Message-ID: <493CAA64F203E14E8823737B9EE0E25F091D4EDE1F@EXCHMB01.isis.local> Are gloves being worn during the whole process, including slide printing? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, September 09, 2011 8:41 AM To: histonet@lists.utsouthwestern.edu Cc: Debra Cobb Subject: [Histonet] Floater Problem I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Fri Sep 9 10:55:17 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Sep 9 10:55:37 2011 Subject: [Histonet] RE: Decal for IHC In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE996815@MERCERMAIL.MercerU.local> 5% formic acid -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laudon, Heather M Sent: Friday, September 09, 2011 11:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal for IHC Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford <@t> CHW.edu Fri Sep 9 10:55:29 2011 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Fri Sep 9 10:55:39 2011 Subject: [Histonet] Traveling HistoTech San Francisco Area Message-ID: <9ECF174E7DA83046BE6EBFDE009E28A388C439@CHW-MSG-301.chw.edu> Hello, I am going to need a ASCP certified Histotech to work for me for about 1.5-2 weeks in the next 2-4 weeks. If you now of a temp. service or someone interested. Call me and leave a message and will get back to you. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From POWELL_SA <@t> mercer.edu Fri Sep 9 10:59:45 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Sep 9 10:59:50 2011 Subject: [Histonet] RE: Floater Problem In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2B0BA@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D76830AD2B0BA@EXCHANGE3.huntingtonhospital.com> Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE99682D@MERCERMAIL.MercerU.local> There is a good article in the JOH, June 2011, Vol 34, Number 2, Gadgets for "Floaters" prevention in the Histopathology Laboratory by Izak Dimenstein that addresses ways to help prevent them. He discusses floater sources in the discussion. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, September 09, 2011 11:41 AM To: histonet@lists.utsouthwestern.edu Cc: Debra Cobb Subject: [Histonet] Floater Problem I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri Sep 9 11:26:56 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Sep 9 11:27:40 2011 Subject: [Histonet] Decal for IHC In-Reply-To: References: Message-ID: Hi Heather, Whatever we use here, if you remember, doesn't compromise IHC results, even for ER/PR. Jamie says its Protocol decalcifier B. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laudon, Heather M Sent: Friday, September 09, 2011 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal for IHC Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Fri Sep 9 12:16:07 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Sep 9 12:16:15 2011 Subject: [Histonet] SC Society of Histotechnology Fall Meeting Program Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA272DF5F112@NADCWPMSGCMS03.hca.corpad.net> HAPPY FRIDAY TO ALL!!!!!!!! For those of you that have requested a brochure for the SCSHT fall meeting, we will be mailing them out next week to the techs on our mailing list. Also continue to email me your request and when I have the brochure in a PDF format, I will email you individually. Thanks for your interest in our meeting and I think it's going to be a GREAT one!!!! Have a good weekend, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From JMacDonald <@t> mtsac.edu Fri Sep 9 12:16:58 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 9 12:30:02 2011 Subject: [Histonet] Floater Problem In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2B0BA@EXCHANGE3.huntingtonhospital.com> Message-ID: Are the floaters present in the paraffin block or just on the slides? "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/09/2011 08:42 AM To cc Debra Cobb Subject [Histonet] Floater Problem I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Fri Sep 9 12:53:12 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Fri Sep 9 12:53:16 2011 Subject: [Histonet] SC Society of Histotechnology Fall Meeting Program In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA272DF5F112@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA272DF5F112@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <1315590792.53230.YahooMailNeo@web120613.mail.ne1.yahoo.com> Wanda - ? I will?mention your meeting again?in the "What's Happening" portion of HistoTALK this Sunday. BTW. Jack Ratiff will be my guest this week. ? Yours Dave From: "Wanda.Smith@HCAhealthcare.com" To: histonet@lists.utsouthwestern.edu Sent: Friday, September 9, 2011 1:16 PM Subject: [Histonet] SC Society of Histotechnology Fall Meeting Program HAPPY FRIDAY TO ALL!!!!!!!! For those of you that have requested a brochure for the SCSHT fall meeting, we will be mailing them out next week to the techs on our mailing list.? Also continue to email me your request and when I have the brochure in a PDF format, I will email you individually. Thanks for your interest in our meeting and I think it's going to be a GREAT one!!!! Have a good weekend, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Fri Sep 9 13:14:49 2011 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Sep 9 13:14:57 2011 Subject: [Histonet] decal In-Reply-To: References: Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43012206913F33@HPEMX3.HealthPartners.int> We use Rapid Cal Immuno (formic acid based) from BBC Biocemical out of Washington. Works great and does not compromise IHC as name suggests! Dorothy Webb -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, September 09, 2011 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 94, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: P63 controls (Troutman, Kenneth A) 2. Full Time HT position in Oklahoma City (Gaiser, Marcia) 3. STP420 vs. Peloris (Davide Costanzo) 4. Seeking new position (Charles O) 5. Comment on HT/HTL (anuradha shrivastava) 6. Facebook (Nicole Tatum) 7. thinprep (tahseen@brain.net.pk) 8. I'm so sad for AFIP to close (Weems, Joyce) 9. RE: I'm so sad for AFIP to close (sdysart@mirnarx.com) 10. RE: I'm so sad for AFIP to close (Edwards, Richard E.) 11. Decal for IHC (Laudon, Heather M) 12. RE: I'm so sad for AFIP to close (Shirley A. Powell) 13. Floater Problem (Laurie Colbert) 14. Re: Decal for IHC (Rene J Buesa) 15. RE: Floater Problem (Bartlett, Jeanine (CDC/OID/NCEZID)) 16. RE: I'm so sad for AFIP to close (Kelly Cross) 17. Re: Floater Problem (Kelly Cross) 18. RE: Floater Problem (Bea DeBrosse-Serra) 19. RE: Decal for IHC (Shirley A. Powell) 20. Traveling HistoTech San Francisco Area (Heckford, Karen - SMMC-SF) 21. RE: Floater Problem (Shirley A. Powell) 22. RE: Decal for IHC (Sebree Linda A) ---------------------------------------------------------------------- Message: 1 Date: Thu, 8 Sep 2011 14:16:21 -0500 From: "Troutman, Kenneth A" Subject: Re: [Histonet] P63 controls To: "Histonet@lists.utsouthwestern.edu" Message-ID: <7B310892042DA74CB3590053F424CFE61441FCEE1C@ITS-HCWNEM06.ds.Vanderbilt.edu> Content-Type: text/plain; charset="us-ascii" We use normal prostate and we have also used skin in the past-they both work well. I would avoid pre-cutting a lot of controls, though; it has a tendency to loose antigenicity. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 9 Date: Thu, 8 Sep 2011 09:08:52 -0400 From: "Sara Baldwin/mhhcc.org" Subject: [Histonet] P63 controls To: Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters We are having trouble with our control for P63 what is everyone using? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ------------------------------ Message: 2 Date: Thu, 8 Sep 2011 15:16:48 -0500 From: "Gaiser, Marcia" Subject: [Histonet] Full Time HT position in Oklahoma City To: "histonet@lists.utsouthwestern.edu" Message-ID: <728F817C02110E498D803A7C3B0C62480690A37B6E@S009-APEXM06.ds.ad.ssmhc.com> Content-Type: text/plain; charset="Windows-1252" POSITION REQUIREMENTS: EDUCATION: High school diploma or GED preferred. CERTIFICATION, LICENSURE, BONDING: Certified as an HT or HLT by the American Society of Clinical Pathologists (ASCP) ? or ? other nationally recognized certifying agency acceptable to the Laboratory Director ? or ? experience acceptable to the Laboratory Director. EXPERIENCE: Two years of satisfactory histology experience. SIGN ON BONUS AVAILABLE FOR QUALIFIED CANDIDATES. Outstanding benefits package including generous paid time off. Apply online at www.saintsok.com>, Ad# 10762, or contact Christina Hughes at (405) 272-6105 for more information. Thank you! Christina Hughes HR Recruiter St. Anthony Hospital (405) 272-6105 - phone (405) 272-6781 - fax Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ ------------------------------ Message: 3 Date: Thu, 8 Sep 2011 13:37:12 -0700 From: Davide Costanzo Subject: [Histonet] STP420 vs. Peloris To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Considering both options. Can any STP users out there give me opinions? Please give reasons, not just "go with" this one. Thanks. ------------------------------ Message: 4 Date: Thu, 8 Sep 2011 17:07:35 -0500 From: Charles O Subject: [Histonet] Seeking new position To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, I am a certified histotech seeking a new position. I have an extensive experience in the general histological technics and in Immunohistochemistry. I am willing to relocate. Thanks, Charles O. ------------------------------ Message: 5 Date: Thu, 8 Sep 2011 20:55:08 -0400 From: anuradha shrivastava Subject: [Histonet] Comment on HT/HTL To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi All, I feel so fortunate that I studied Histo in Cobleskill, with my bio background It was perfect. I think the science sub. background is also needed specially anatomy, so that one knows how to embed different tissues. I hope u all will agree with me. Slide submittion should be there in the exam. Anu Shrivastava. M.S , HTL (ASCP) Histology Team Leader, South County Hospital RI ------------------------------ Message: 6 Date: Fri, 9 Sep 2011 09:21:06 -0400 (EDT) From: "Nicole Tatum" Subject: [Histonet] Facebook To: histonet@lists.utsouthwestern.edu Message-ID: <4934.208.62.167.196.1315574466.squirrel@webmail.realpages.com> Content-Type: text/plain;charset=iso-8859-1 Hey histonetters, Ive created a community page for histologist on Facebook. Please join or like Histo-ville. It a page for people to make friends, troubleshoot, or discuss issues. I hope to create a large community of friends. So stop by and say hi. I look forward to meeting fellow techs. Nicole Tatum, HT ASCP ------------------------------ Message: 7 Date: Fri, 9 Sep 2011 18:34:25 +0500 (PKT) From: tahseen@brain.net.pk Subject: [Histonet] thinprep To: histonet@lists.utsouthwestern.edu Cc: cyto@skm.org.pk Message-ID: <62815.203.135.35.66.1315575265.squirrel@brain.net.pk> Content-Type: text/plain;charset=iso-8859-1 We want to purchase a latest model machine for thin prep/sure path along with an auto stainer for pap.stain. Can you share your experience regarding both types of machines, 1) Which type of system is best 2)which machine will provide an auto stainer 3)which model of the recommended machine is best 4)will they arrange for training of cytotechnologist and pathologist for processing and reportting of specimen 5)which company is providing best after sale service Thanks ------------------------------ Message: 8 Date: Fri, 9 Sep 2011 10:33:42 -0400 From: "Weems, Joyce" Subject: [Histonet] I'm so sad for AFIP to close To: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8BBEB@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 9 Date: Fri, 9 Sep 2011 10:02:43 -0500 From: Subject: RE: [Histonet] I'm so sad for AFIP to close To: , Message-ID: Content-Type: text/plain; charset="us-ascii" Wow...that is sad news. Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat hology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 9 Sep 2011 16:07:32 +0100 From: "Edwards, Richard E." Subject: RE: [Histonet] I'm so sad for AFIP to close To: "'sdysart@mirnarx.com'" , "JWeems@sjha.org" , "histonet@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4EC22F6DF@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Well unfortunately one can't have public/governmental spending cut backs without losing something important along the way. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sdysart@mirnarx.com Sent: 09 September 2011 16:03 To: JWeems@sjha.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] I'm so sad for AFIP to close Wow...that is sad news. Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat hology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 9 Sep 2011 15:23:16 +0000 From: "Laudon, Heather M" Subject: [Histonet] Decal for IHC To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 ------------------------------ Message: 12 Date: Fri, 9 Sep 2011 11:32:47 -0400 From: "Shirley A. Powell" Subject: [Histonet] RE: I'm so sad for AFIP to close To: "Weems, Joyce" , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE9967AE@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" I know, just breaks your heart. I spent 3 weeks there in 19...... well a long time ago. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Fri, 9 Sep 2011 08:40:48 -0700 From: "Laurie Colbert" Subject: [Histonet] Floater Problem To: Cc: Debra Cobb Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2B0BA@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert ------------------------------ Message: 14 Date: Fri, 9 Sep 2011 08:45:54 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Decal for IHC To: "histonet@lists.utsouthwestern.edu" , Heather MLaudon Message-ID: <1315583154.12504.YahooMailClassic@web65711.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 EDTA pH 7 Ren? J. --- On Fri, 9/9/11, Laudon, Heather M wrote: From: Laudon, Heather M Subject: [Histonet] Decal for IHC To: "histonet@lists.utsouthwestern.edu" Date: Friday, September 9, 2011, 11:23 AM Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Fri, 9 Sep 2011 15:45:08 +0000 From: "Bartlett, Jeanine (CDC/OID/NCEZID)" Subject: [Histonet] RE: Floater Problem To: Laurie Colbert , "histonet@lists.utsouthwestern.edu" Cc: Debra Cobb Message-ID: Content-Type: text/plain; charset="us-ascii" I have also heard of the occasional floater coming from the processor and making its way into the paraffin block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, September 09, 2011 11:41 AM To: histonet@lists.utsouthwestern.edu Cc: Debra Cobb Subject: [Histonet] Floater Problem I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Fri, 09 Sep 2011 10:49:29 -0500 From: "Kelly Cross" Subject: [Histonet] RE: I'm so sad for AFIP to close To: "histonet@lists.utsouthwestern.edu" , "Shirley A. Powell" , "Joyce Weems" Message-ID: <4E69EF39020000E3000DAE6A@CVM.TAMU.EDU> Content-Type: text/plain; charset=US-ASCII As a young histotech I felt I had "arrived" when my supervisor assigned me to take care of the AFIP study set years ago! It is sad... Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Texas A&M University Veterinary Pathobiology VMA bldg. 207B College Station, TX 77843-4467 Office: 979-862-3658 Lab: 979-845-5149 Fax: 979-862-1147 kcross@cvm.tamu.edu >>> "Shirley A. Powell" 9/9/2011 10:32 AM >>> I know, just breaks your heart. I spent 3 weeks there in 19...... well a long time ago. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Fri, 09 Sep 2011 10:50:32 -0500 From: "Kelly Cross" Subject: Re: [Histonet] Floater Problem To: "Laurie Colbert" , Cc: Debra Cobb Message-ID: <4E69EF78020000E3000DAE71@CVM.TAMU.EDU> Content-Type: text/plain; charset=US-ASCII Water bath???? Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Texas A&M University Veterinary Pathobiology VMA bldg. 207B College Station, TX 77843-4467 Office: 979-862-3658 Lab: 979-845-5149 Fax: 979-862-1147 kcross@cvm.tamu.edu >>> "Laurie Colbert" 9/9/2011 10:40 AM >>> I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Fri, 9 Sep 2011 08:52:52 -0700 From: Bea DeBrosse-Serra Subject: [Histonet] RE: Floater Problem To: "'Laurie Colbert'" , "histonet@lists.utsouthwestern.edu" Cc: Debra Cobb Message-ID: <493CAA64F203E14E8823737B9EE0E25F091D4EDE1F@EXCHMB01.isis.local> Content-Type: text/plain; charset="us-ascii" Are gloves being worn during the whole process, including slide printing? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, September 09, 2011 8:41 AM To: histonet@lists.utsouthwestern.edu Cc: Debra Cobb Subject: [Histonet] Floater Problem I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Fri, 9 Sep 2011 11:55:17 -0400 From: "Shirley A. Powell" Subject: [Histonet] RE: Decal for IHC To: "Laudon, Heather M" , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE996815@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" 5% formic acid -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laudon, Heather M Sent: Friday, September 09, 2011 11:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal for IHC Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Fri, 9 Sep 2011 08:55:29 -0700 From: "Heckford, Karen - SMMC-SF" Subject: [Histonet] Traveling HistoTech San Francisco Area To: Message-ID: <9ECF174E7DA83046BE6EBFDE009E28A388C439@CHW-MSG-301.chw.edu> Content-Type: text/plain; charset="us-ascii" Hello, I am going to need a ASCP certified Histotech to work for me for about 1.5-2 weeks in the next 2-4 weeks. If you now of a temp. service or someone interested. Call me and leave a message and will get back to you. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ------------------------------ Message: 21 Date: Fri, 9 Sep 2011 11:59:45 -0400 From: "Shirley A. Powell" Subject: [Histonet] RE: Floater Problem To: Laurie Colbert , "histonet@lists.utsouthwestern.edu" Cc: Debra Cobb Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE99682D@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" There is a good article in the JOH, June 2011, Vol 34, Number 2, Gadgets for "Floaters" prevention in the Histopathology Laboratory by Izak Dimenstein that addresses ways to help prevent them. He discusses floater sources in the discussion. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, September 09, 2011 11:41 AM To: histonet@lists.utsouthwestern.edu Cc: Debra Cobb Subject: [Histonet] Floater Problem I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Fri, 9 Sep 2011 11:26:56 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] Decal for IHC To: "Laudon, Heather M" , Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Heather, Whatever we use here, if you remember, doesn't compromise IHC results, even for ER/PR. Jamie says its Protocol decalcifier B. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laudon, Heather M Sent: Friday, September 09, 2011 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal for IHC Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 94, Issue 9 *************************************** This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From foreightl <@t> gmail.com Fri Sep 9 13:32:25 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Fri Sep 9 13:32:30 2011 Subject: [Histonet] Floater Problem In-Reply-To: References: <57BE698966D5C54EAE8612E8941D76830AD2B0BA@EXCHANGE3.huntingtonhospital.com> Message-ID: We had a similar project. I found that in my organization, floaters have 3 main sources and 1 or 2 occasional sources. 1. Embedding forceps/forceps warming wells, 2. Grossing instruments and cutting boards and 3. Dirty waterbaths. It is the first two that really cause problems, these are at the same level as the tissue you are looking at, dirty waterbath floaters can be discounted by recutting the block. We have a system that records when blocks are grossed, cut and embedded so we can usually tell which case was the source of the floater. I also got forceps without teeth for the embedding station, and one of those microbiology inoculation loop sterilizers. Our level of floaters has significantly decreased. Good luck. On Fri, Sep 9, 2011 at 10:16 AM, Jennifer MacDonald wrote: > Are the floaters present in the paraffin block or just on the slides? > > > > > "Laurie Colbert" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 09/09/2011 08:42 AM > > To > > cc > Debra Cobb > Subject > [Histonet] Floater Problem > > > > > > > I am starting a PI project concerning floaters on slides. I am > collecting data and determining whether the floater is in the block or > on the slide. If the floater is in the block, it either came from the > grossing step or the embedding step. Of course, both the PA and the > embedders swear that they clean their forceps and their work surfaces > diligently. My problem is in determining where the floater actually > came from. Any suggestions?? > > > > Thanks, > > Laurie Colbert > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From kim.tournear <@t> yahoo.com Fri Sep 9 13:39:42 2011 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Fri Sep 9 13:39:47 2011 Subject: [Histonet] I'm so sad for AFIP to close Message-ID: <1315593582.77625.yint-ygo-j2me@web120216.mail.ne1.yahoo.com> Actually, I heard the program moved to Ft. Sam Houston in San Antonio, TX. On Fri Sep 9th, 2011 8:07 AM PDT Edwards, Richard E. wrote: >Well unfortunately one can't have public/governmental spending cut backs without losing something important along the way. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sdysart@mirnarx.com >Sent: 09 September 2011 16:03 >To: JWeems@sjha.org; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] I'm so sad for AFIP to close > >Wow...that is sad news. > >Sarah Goebel-Dysart, BA, HT(ASCP) >Histotechnologist >Mirna Therapeutics >2150 Woodward Street >Suite 100 >Austin, Texas 78744 >(512)901-0900 ext. 6912 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, >Joyce >Sent: Friday, September 09, 2011 9:34 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] I'm so sad for AFIP to close > >I never really thought it would happen but it has... > >http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat >hology-Community.html > > > >Joyce Weems >Pathology Manager >Saint Joseph's Hospital >5665 Peachtree Dunwoody Rd NE >Atlanta, GA 30342 >678-843-7376 - Phone >678-843-7831 - Fax > > >Confidentiality Notice: >This e-mail, including any attachments is the >property of Catholic Health East and is intended >for the sole use of the intended recipient(s). >It may contain information that is privileged and >confidential. Any unauthorized review, use, >disclosure, or distribution is prohibited. If you are >not the intended recipient, please delete this message, and >reply to the sender regarding the error in a separate email. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Fri Sep 9 14:09:26 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 9 14:09:31 2011 Subject: [Histonet] I'm so sad for AFIP to close References: <1315593582.77625.yint-ygo-j2me@web120216.mail.ne1.yahoo.com> Message-ID: <065DDA0E28564E4BAC620FE1C6166859@auxs.umn.edu> The (Veterinary Pathology) Wednesday Slide Conferences are now organized under the Joint Pathology Center, so at least that service of providing histologic pathology study slides is still available. http://wsc.jpc.capmed.mil/dodvpr/wsc.php Jan Shivers UMN VDL ----- Original Message ----- From: "Kim Tournear" To: Sent: Friday, September 09, 2011 1:39 PM Subject: RE: [Histonet] I'm so sad for AFIP to close > Actually, I heard the program moved to Ft. Sam Houston in San Antonio, TX. > > On Fri Sep 9th, 2011 8:07 AM PDT Edwards, Richard E. wrote: > >>Well unfortunately one can't have public/governmental spending cut backs >>without losing something important along the way. >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >>sdysart@mirnarx.com >>Sent: 09 September 2011 16:03 >>To: JWeems@sjha.org; histonet@lists.utsouthwestern.edu >>Subject: RE: [Histonet] I'm so sad for AFIP to close >> >>Wow...that is sad news. >> >>Sarah Goebel-Dysart, BA, HT(ASCP) >>Histotechnologist >>Mirna Therapeutics >>2150 Woodward Street >>Suite 100 >>Austin, Texas 78744 >>(512)901-0900 ext. 6912 >> >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, >>Joyce >>Sent: Friday, September 09, 2011 9:34 AM >>To: histonet@lists.utsouthwestern.edu >>Subject: [Histonet] I'm so sad for AFIP to close >> >>I never really thought it would happen but it has... >> >>http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat >>hology-Community.html >> >> >> >>Joyce Weems >>Pathology Manager >>Saint Joseph's Hospital >>5665 Peachtree Dunwoody Rd NE >>Atlanta, GA 30342 >>678-843-7376 - Phone >>678-843-7831 - Fax >> >> >>Confidentiality Notice: >>This e-mail, including any attachments is the >>property of Catholic Health East and is intended >>for the sole use of the intended recipient(s). >>It may contain information that is privileged and >>confidential. Any unauthorized review, use, >>disclosure, or distribution is prohibited. If you are >>not the intended recipient, please delete this message, and >>reply to the sender regarding the error in a separate email. >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From POWELL_SA <@t> mercer.edu Fri Sep 9 14:19:14 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Sep 9 14:19:20 2011 Subject: [Histonet] GSH 2012 meeting Message-ID: <9BF995BC0E47744E9673A41486E24EE238DEA341AA@MERCERMAIL.MercerU.local> Hi Everyone, Just wanted to let you know we have a great lineup of speakers for the Georgia Society for Histotechnology meeting in 2012 when we are hosting the Region III. It will be at Callaway Gardens, the Mountain Creek Inn in Pine Mountain GA April 13-15th. The program will be finalized soon and up on our website at www.histosearch.com/gsh very soon. The flyer with the hotel information is already there except for the GSH group #. I urge you to make your reservations now, the sooner the better, at 1800-225-5292. Spring is a popular time at Callaway and the rooms fill up fast. Be sure to use the GSH group # 78K711 to get the discounted room rate of $109. The price of your room will get you access to the gardens. Your credit card will NOT be billed until you register at the hotel in April 2012. There are only a certain number of rooms blocked so don't delay. Our speaker lineup to date is below. This is a great way to get your CEUs and also to visit a wonderful southern jewel. There is something for everyone and April will be a gorgeous month for our meeting. The gardens are in bloom, the golf courses are outstanding and there is a beach for the kids. The butterfly pavilion is always a big hit with everyone. To date our speakers and topics are: Taiquanda Winbush- HT/HTL Review Adrianna Eaton - Antibody Challenge Claudia Lawson - Advances in Molecular Testing Joyce Weems - CPT Coding - Sweet Dream or Nightmare? Marvin Hanna - The Molecular Pathology Lab of the Future Ada Feldman - Troubleshooting Hematoxylin and Eosin Staining Wanda Jones - IHC Controls---The Good, The Bad and The Ugly! Ada Feldman - The Joy of Histology or What We Can Learn from the Kitchen Steven Westra - Get the most out of your Immunohistochemistry: A Balance between Convenience, Cost, Education and Flexibility Lamar Jones - Real Time Rapid Tissue Processing Ely Klar - Human Tissues: Histological Identification of the Different Types So come to Georgia in April 2012, it is going to be a Histo-terrific Meeting. Shirley A. Powell, HT(ASCP)HTL, QIHC GSH Secretary 478-301-2374 Lab 478-301-5489 Fax From diane.gladney <@t> us.army.mil Fri Sep 9 14:41:18 2011 From: diane.gladney <@t> us.army.mil (Gladney, Diane C Ms CIV USA MEDCOM MACH) Date: Fri Sep 9 14:41:29 2011 Subject: [Histonet] I'm so sad for AFIP to close (UNCLASSIFIED) In-Reply-To: <1315593582.77625.yint-ygo-j2me@web120216.mail.ne1.yahoo.com> References: <1315593582.77625.yint-ygo-j2me@web120216.mail.ne1.yahoo.com> Message-ID: Classification: UNCLASSIFIED Caveats: NONE The Joint Pathology Center in Silver Springs, Maryland has replaced AFIP. They started accepting consultations in April 2011. The Joint Pathology Center is for Department of Defense and other government agencies. Below is the link to their website: http://www.jpc.capmed.mil/index.asp They do have a veterinary department, too. Have A Great Weekend! Diane C. Gladney, HT (ASCP) Histology Supervisor Department of Pathology Moncrief Army Community Hospital 4500 Stuart Street FT. Jackson, SC 29207 Phone: 803-751-2530 Fax: 803-751-7829 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Friday, September 09, 2011 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] I'm so sad for AFIP to close Actually, I heard the program moved to Ft. Sam Houston in San Antonio, TX. On Fri Sep 9th, 2011 8:07 AM PDT Edwards, Richard E. wrote: >Well unfortunately one can't have public/governmental spending cut backs without losing something important along the way. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sdysart@mirnarx.com >Sent: 09 September 2011 16:03 >To: JWeems@sjha.org; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] I'm so sad for AFIP to close > >Wow...that is sad news. > >Sarah Goebel-Dysart, BA, HT(ASCP) >Histotechnologist >Mirna Therapeutics >2150 Woodward Street >Suite 100 >Austin, Texas 78744 >(512)901-0900 ext. 6912 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, >Joyce >Sent: Friday, September 09, 2011 9:34 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] I'm so sad for AFIP to close > >I never really thought it would happen but it has... > >http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat >hology-Community.html > > > >Joyce Weems >Pathology Manager >Saint Joseph's Hospital >5665 Peachtree Dunwoody Rd NE >Atlanta, GA 30342 >678-843-7376 - Phone >678-843-7831 - Fax > > >Confidentiality Notice: >This e-mail, including any attachments is the >property of Catholic Health East and is intended >for the sole use of the intended recipient(s). >It may contain information that is privileged and >confidential. Any unauthorized review, use, >disclosure, or distribution is prohibited. If you are >not the intended recipient, please delete this message, and >reply to the sender regarding the error in a separate email. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE From ksverlow <@t> cahfs.ucdavis.edu Fri Sep 9 14:45:49 2011 From: ksverlow <@t> cahfs.ucdavis.edu (Sverlow, Karen) Date: Fri Sep 9 14:45:55 2011 Subject: [Histonet] Bovine corona virus antibody Message-ID: I am looking for antibody recommendations for our bovine corona virus IHC. The clone we used previously is no longer available. Thanks, Karen Sverlow, HTL (ASCP)CM Histology Supervisor California Animal Health and Food Safety Laboratory System Thurman Building, West Health Sciences Drive Davis, CA 95616 530-752-8753 From shive003 <@t> umn.edu Fri Sep 9 14:57:32 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 9 14:57:35 2011 Subject: [Histonet] Bovine corona virus antibody References: Message-ID: <2852208A1C5A468AA7B76DE6BB13D0FA@auxs.umn.edu> Mouse anti-BCV from Rural Technologies, Inc (RTI); clone BC 6-4. I use my current lot at around 1:5000 with HIER (Decloaking Chamber). If you use another method of antigen retrieval, drop the dilution down quite a bit. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "Sverlow, Karen" To: Sent: Friday, September 09, 2011 2:45 PM Subject: [Histonet] Bovine corona virus antibody I am looking for antibody recommendations for our bovine corona virus IHC. The clone we used previously is no longer available. Thanks, Karen Sverlow, HTL (ASCP)CM Histology Supervisor California Animal Health and Food Safety Laboratory System Thurman Building, West Health Sciences Drive Davis, CA 95616 530-752-8753 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dliaros <@t> BioReference.com Fri Sep 9 15:05:59 2011 From: dliaros <@t> BioReference.com (Donna Liaros) Date: Fri Sep 9 15:06:14 2011 Subject: [Histonet] MYOD1 Message-ID: <6CB019913E5F6940AD6E4871841EC5364B5828@MBX-2.biosvr1.bioreference.com> Any protocol recommendations for MYOD1? I am having a difficult time in trying to work up this antibody. The information transmitted in this email and any of its attachments is intended only for the person or entity to which it is addressed and may contain BioReference Laboratories proprietary information, which is privileged, confidential, or subject to copyright belonging to BioReference Laboratories. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited and may be unlawful. If you received this in error, please contact the sender immediately and delete and destroy the communication and all of the attachments you have received and all copies thereof. From TGoins <@t> mt.gov Fri Sep 9 16:37:18 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Sep 9 16:37:25 2011 Subject: [Histonet] RE: Bovine corona virus antibody In-Reply-To: References: Message-ID: BCV clone BC-8F2-C from RTI - retrieve with trypsin digestion for 15 min - our titer is 1:250 but we have used the same lot for more than five years. Tresa Goins Veterinary Diagnostic Lab South 19th and Lincoln Bozeman, MT 59718 406-994-6353 - phone 406-994-6344 - fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sverlow, Karen Sent: Friday, September 09, 2011 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bovine corona virus antibody I am looking for antibody recommendations for our bovine corona virus IHC. The clone we used previously is no longer available. Thanks, Karen Sverlow, HTL (ASCP)CM Histology Supervisor California Animal Health and Food Safety Laboratory System Thurman Building, West Health Sciences Drive Davis, CA 95616 530-752-8753 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diane.Tokugawa <@t> kp.org Fri Sep 9 18:02:53 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Fri Sep 9 18:03:16 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 09/09/2011 and will not return until 09/14/2011. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology issues, please call Mario at 8-421-4961, general histology lab 8-421- 5408 or Wanda at 8-421-5426. From SteveM <@t> mcclainlab.com Fri Sep 9 19:15:04 2011 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Fri Sep 9 19:02:26 2011 Subject: [Histonet] RE: Almost Eliminating Floaters Message-ID: Floaters on the water bath are generally not the problem, are usually identifiable, unlike the serious issue posed by floaters in the block. I would suggest counting to get a benchmark. In order to change the outcome, you (the institutional you) must change what you are doing. SO change your grossing-procedure. Start by scrupulously cleaning every pair of forceps. Keep an open vial of 50% alcohol at the bench to have the grossers rinse their forceps between specimens. Photograph every specimen-this forces the grossers to maintain a clean background. If you do not have grossing cameras, then mount a video camera above the grossing station and let it run. Point is a few hours study of technique can help identify where the problem is. Better than wasting many hours. Have grossers begin to wrap every small specimen in lens paper- Three darn good reasons. Wrapping specimens with forceps helps to clean the forceps before the next specimen. At embedding , the action of opening/unwrapping specimens with forceps cleans forceps at the embedding center. Floaters can also occur during processing-wrapping specimens prevents floaters during processing and keeps processors clean. (If you don't believe me- filter your first reagent when changing and then process that to a block- I once inherited a processor from a lab that did placentas. We found pieces of placenta in our derm specimens as long as 5 or 6 months later.) Change fixative and clean block bucket at the grossing bench routinely. You may wish to consider rinsing all blocks in fresh reagent BEFORE placing on processor. Evolving to this procedure, the floaters fell to 1 per 15,000, about 3 per year in our small lab Steve A. McClain, MD 631 361 4000 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, September 09, 2011 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 94, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: P63 controls (Troutman, Kenneth A) 2. Full Time HT position in Oklahoma City (Gaiser, Marcia) 3. STP420 vs. Peloris (Davide Costanzo) 4. Seeking new position (Charles O) 5. Comment on HT/HTL (anuradha shrivastava) 6. Facebook (Nicole Tatum) 7. thinprep (tahseen@brain.net.pk) 8. I'm so sad for AFIP to close (Weems, Joyce) 9. RE: I'm so sad for AFIP to close (sdysart@mirnarx.com) 10. RE: I'm so sad for AFIP to close (Edwards, Richard E.) 11. Decal for IHC (Laudon, Heather M) 12. RE: I'm so sad for AFIP to close (Shirley A. Powell) 13. Floater Problem (Laurie Colbert) 14. Re: Decal for IHC (Rene J Buesa) 15. RE: Floater Problem (Bartlett, Jeanine (CDC/OID/NCEZID)) 16. RE: I'm so sad for AFIP to close (Kelly Cross) 17. Re: Floater Problem (Kelly Cross) 18. RE: Floater Problem (Bea DeBrosse-Serra) 19. RE: Decal for IHC (Shirley A. Powell) 20. Traveling HistoTech San Francisco Area (Heckford, Karen - SMMC-SF) 21. RE: Floater Problem (Shirley A. Powell) 22. RE: Decal for IHC (Sebree Linda A) ---------------------------------------------------------------------- Message: 1 Date: Thu, 8 Sep 2011 14:16:21 -0500 From: "Troutman, Kenneth A" Subject: Re: [Histonet] P63 controls To: "Histonet@lists.utsouthwestern.edu" Message-ID: <7B310892042DA74CB3590053F424CFE61441FCEE1C@ITS-HCWNEM06.ds.Vanderbilt.edu> Content-Type: text/plain; charset="us-ascii" We use normal prostate and we have also used skin in the past-they both work well. I would avoid pre-cutting a lot of controls, though; it has a tendency to loose antigenicity. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 9 Date: Thu, 8 Sep 2011 09:08:52 -0400 From: "Sara Baldwin/mhhcc.org" Subject: [Histonet] P63 controls To: Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters We are having trouble with our control for P63 what is everyone using? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ------------------------------ Message: 2 Date: Thu, 8 Sep 2011 15:16:48 -0500 From: "Gaiser, Marcia" Subject: [Histonet] Full Time HT position in Oklahoma City To: "histonet@lists.utsouthwestern.edu" Message-ID: <728F817C02110E498D803A7C3B0C62480690A37B6E@S009-APEXM06.ds.ad.ssmhc.com> Content-Type: text/plain; charset="Windows-1252" POSITION REQUIREMENTS: EDUCATION: High school diploma or GED preferred. CERTIFICATION, LICENSURE, BONDING: Certified as an HT or HLT by the American Society of Clinical Pathologists (ASCP) - or - other nationally recognized certifying agency acceptable to the Laboratory Director - or - experience acceptable to the Laboratory Director. EXPERIENCE: Two years of satisfactory histology experience. SIGN ON BONUS AVAILABLE FOR QUALIFIED CANDIDATES. Outstanding benefits package including generous paid time off. Apply online at www.saintsok.com>, Ad# 10762, or contact Christina Hughes at (405) 272-6105 for more information. Thank you! Christina Hughes HR Recruiter St. Anthony Hospital (405) 272-6105 - phone (405) 272-6781 - fax Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ ------------------------------ Message: 3 Date: Thu, 8 Sep 2011 13:37:12 -0700 From: Davide Costanzo Subject: [Histonet] STP420 vs. Peloris To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Considering both options. Can any STP users out there give me opinions? Please give reasons, not just "go with" this one. Thanks. ------------------------------ Message: 4 Date: Thu, 8 Sep 2011 17:07:35 -0500 From: Charles O Subject: [Histonet] Seeking new position To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, I am a certified histotech seeking a new position. I have an extensive experience in the general histological technics and in Immunohistochemistry. I am willing to relocate. Thanks, Charles O. ------------------------------ Message: 5 Date: Thu, 8 Sep 2011 20:55:08 -0400 From: anuradha shrivastava Subject: [Histonet] Comment on HT/HTL To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi All, I feel so fortunate that I studied Histo in Cobleskill, with my bio background It was perfect. I think the science sub. background is also needed specially anatomy, so that one knows how to embed different tissues. I hope u all will agree with me. Slide submittion should be there in the exam. Anu Shrivastava. M.S , HTL (ASCP) Histology Team Leader, South County Hospital RI ------------------------------ Message: 6 Date: Fri, 9 Sep 2011 09:21:06 -0400 (EDT) From: "Nicole Tatum" Subject: [Histonet] Facebook To: histonet@lists.utsouthwestern.edu Message-ID: <4934.208.62.167.196.1315574466.squirrel@webmail.realpages.com> Content-Type: text/plain;charset=iso-8859-1 Hey histonetters, Ive created a community page for histologist on Facebook. Please join or like Histo-ville. It a page for people to make friends, troubleshoot, or discuss issues. I hope to create a large community of friends. So stop by and say hi. I look forward to meeting fellow techs. Nicole Tatum, HT ASCP ------------------------------ Message: 7 Date: Fri, 9 Sep 2011 18:34:25 +0500 (PKT) From: tahseen@brain.net.pk Subject: [Histonet] thinprep To: histonet@lists.utsouthwestern.edu Cc: cyto@skm.org.pk Message-ID: <62815.203.135.35.66.1315575265.squirrel@brain.net.pk> Content-Type: text/plain;charset=iso-8859-1 We want to purchase a latest model machine for thin prep/sure path along with an auto stainer for pap.stain. Can you share your experience regarding both types of machines, 1) Which type of system is best 2)which machine will provide an auto stainer 3)which model of the recommended machine is best 4)will they arrange for training of cytotechnologist and pathologist for processing and reportting of specimen 5)which company is providing best after sale service Thanks ------------------------------ Message: 8 Date: Fri, 9 Sep 2011 10:33:42 -0400 From: "Weems, Joyce" Subject: [Histonet] I'm so sad for AFIP to close To: "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640827A8BBEB@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 9 Date: Fri, 9 Sep 2011 10:02:43 -0500 From: Subject: RE: [Histonet] I'm so sad for AFIP to close To: , Message-ID: Content-Type: text/plain; charset="us-ascii" Wow...that is sad news. Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat hology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 9 Sep 2011 16:07:32 +0100 From: "Edwards, Richard E." Subject: RE: [Histonet] I'm so sad for AFIP to close To: "'sdysart@mirnarx.com'" , "JWeems@sjha.org" , "histonet@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4EC22F6DF@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Well unfortunately one can't have public/governmental spending cut backs without losing something important along the way. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sdysart@mirnarx.com Sent: 09 September 2011 16:03 To: JWeems@sjha.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] I'm so sad for AFIP to close Wow...that is sad news. Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat hology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 9 Sep 2011 15:23:16 +0000 From: "Laudon, Heather M" Subject: [Histonet] Decal for IHC To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 ------------------------------ Message: 12 Date: Fri, 9 Sep 2011 11:32:47 -0400 From: "Shirley A. Powell" Subject: [Histonet] RE: I'm so sad for AFIP to close To: "Weems, Joyce" , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE9967AE@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" I know, just breaks your heart. I spent 3 weeks there in 19...... well a long time ago. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Fri, 9 Sep 2011 08:40:48 -0700 From: "Laurie Colbert" Subject: [Histonet] Floater Problem To: Cc: Debra Cobb Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2B0BA@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert ------------------------------ Message: 14 Date: Fri, 9 Sep 2011 08:45:54 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Decal for IHC To: "histonet@lists.utsouthwestern.edu" , Heather MLaudon Message-ID: <1315583154.12504.YahooMailClassic@web65711.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 EDTA pH 7 Ren? J. --- On Fri, 9/9/11, Laudon, Heather M wrote: From: Laudon, Heather M Subject: [Histonet] Decal for IHC To: "histonet@lists.utsouthwestern.edu" Date: Friday, September 9, 2011, 11:23 AM Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Fri, 9 Sep 2011 15:45:08 +0000 From: "Bartlett, Jeanine (CDC/OID/NCEZID)" Subject: [Histonet] RE: Floater Problem To: Laurie Colbert , "histonet@lists.utsouthwestern.edu" Cc: Debra Cobb Message-ID: Content-Type: text/plain; charset="us-ascii" I have also heard of the occasional floater coming from the processor and making its way into the paraffin block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, September 09, 2011 11:41 AM To: histonet@lists.utsouthwestern.edu Cc: Debra Cobb Subject: [Histonet] Floater Problem I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Fri, 09 Sep 2011 10:49:29 -0500 From: "Kelly Cross" Subject: [Histonet] RE: I'm so sad for AFIP to close To: "histonet@lists.utsouthwestern.edu" , "Shirley A. Powell" , "Joyce Weems" Message-ID: <4E69EF39020000E3000DAE6A@CVM.TAMU.EDU> Content-Type: text/plain; charset=US-ASCII As a young histotech I felt I had "arrived" when my supervisor assigned me to take care of the AFIP study set years ago! It is sad... Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Texas A&M University Veterinary Pathobiology VMA bldg. 207B College Station, TX 77843-4467 Office: 979-862-3658 Lab: 979-845-5149 Fax: 979-862-1147 kcross@cvm.tamu.edu >>> "Shirley A. Powell" 9/9/2011 10:32 AM >>> I know, just breaks your heart. I spent 3 weeks there in 19...... well a long time ago. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Fri, 09 Sep 2011 10:50:32 -0500 From: "Kelly Cross" Subject: Re: [Histonet] Floater Problem To: "Laurie Colbert" , Cc: Debra Cobb Message-ID: <4E69EF78020000E3000DAE71@CVM.TAMU.EDU> Content-Type: text/plain; charset=US-ASCII Water bath???? Kelly S. Cross B.S., HT (ASCP) Medical Laboratory Supervisor Texas A&M University Veterinary Pathobiology VMA bldg. 207B College Station, TX 77843-4467 Office: 979-862-3658 Lab: 979-845-5149 Fax: 979-862-1147 kcross@cvm.tamu.edu >>> "Laurie Colbert" 9/9/2011 10:40 AM >>> I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Fri, 9 Sep 2011 08:52:52 -0700 From: Bea DeBrosse-Serra Subject: [Histonet] RE: Floater Problem To: "'Laurie Colbert'" , "histonet@lists.utsouthwestern.edu" Cc: Debra Cobb Message-ID: <493CAA64F203E14E8823737B9EE0E25F091D4EDE1F@EXCHMB01.isis.local> Content-Type: text/plain; charset="us-ascii" Are gloves being worn during the whole process, including slide printing? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, September 09, 2011 8:41 AM To: histonet@lists.utsouthwestern.edu Cc: Debra Cobb Subject: [Histonet] Floater Problem I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Fri, 9 Sep 2011 11:55:17 -0400 From: "Shirley A. Powell" Subject: [Histonet] RE: Decal for IHC To: "Laudon, Heather M" , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE996815@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" 5% formic acid -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laudon, Heather M Sent: Friday, September 09, 2011 11:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal for IHC Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Fri, 9 Sep 2011 08:55:29 -0700 From: "Heckford, Karen - SMMC-SF" Subject: [Histonet] Traveling HistoTech San Francisco Area To: Message-ID: <9ECF174E7DA83046BE6EBFDE009E28A388C439@CHW-MSG-301.chw.edu> Content-Type: text/plain; charset="us-ascii" Hello, I am going to need a ASCP certified Histotech to work for me for about 1.5-2 weeks in the next 2-4 weeks. If you now of a temp. service or someone interested. Call me and leave a message and will get back to you. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ------------------------------ Message: 21 Date: Fri, 9 Sep 2011 11:59:45 -0400 From: "Shirley A. Powell" Subject: [Histonet] RE: Floater Problem To: Laurie Colbert , "histonet@lists.utsouthwestern.edu" Cc: Debra Cobb Message-ID: <9BF995BC0E47744E9673A41486E24EE238DE99682D@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" There is a good article in the JOH, June 2011, Vol 34, Number 2, Gadgets for "Floaters" prevention in the Histopathology Laboratory by Izak Dimenstein that addresses ways to help prevent them. He discusses floater sources in the discussion. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, September 09, 2011 11:41 AM To: histonet@lists.utsouthwestern.edu Cc: Debra Cobb Subject: [Histonet] Floater Problem I am starting a PI project concerning floaters on slides. I am collecting data and determining whether the floater is in the block or on the slide. If the floater is in the block, it either came from the grossing step or the embedding step. Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Fri, 9 Sep 2011 11:26:56 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] Decal for IHC To: "Laudon, Heather M" , Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Heather, Whatever we use here, if you remember, doesn't compromise IHC results, even for ER/PR. Jamie says its Protocol decalcifier B. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laudon, Heather M Sent: Friday, September 09, 2011 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal for IHC Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help! Heather Laudon, HTL Histology Technician Surgical Pathology Laboratory Gundersen Lutheran Hospital 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 94, Issue 9 *************************************** From lowman034 <@t> yahoo.com Fri Sep 9 20:00:45 2011 From: lowman034 <@t> yahoo.com (Dave Low) Date: Fri Sep 9 20:00:49 2011 Subject: [Histonet] I'm so sad for AFIP to close In-Reply-To: <1315593582.77625.yint-ygo-j2me@web120216.mail.ne1.yahoo.com> References: <1315593582.77625.yint-ygo-j2me@web120216.mail.ne1.yahoo.com> Message-ID: <1315616445.77613.YahooMailNeo@web65920.mail.ac4.yahoo.com> Kim, ? The Tri-Service Histopathology School moved to Ft. Sam Houston. ? Dave From: Kim Tournear To: histonet@lists.utsouthwestern.edu Sent: Friday, September 9, 2011 2:39 PM Subject: RE: [Histonet] I'm so sad for AFIP to close Actually, I heard the program moved to Ft. Sam Houston in San Antonio, TX. On Fri Sep 9th, 2011 8:07 AM PDT Edwards, Richard E. wrote: >Well unfortunately one can't have public/governmental spending cut backs without losing something important along the way. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sdysart@mirnarx.com >Sent: 09 September 2011 16:03 >To: JWeems@sjha.org; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] I'm so sad for AFIP to close > >Wow...that is sad news. > >Sarah Goebel-Dysart, BA, HT(ASCP) >Histotechnologist >Mirna Therapeutics >2150 Woodward Street >Suite 100 >Austin, Texas? 78744 >(512)901-0900 ext. 6912 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, >Joyce >Sent: Friday, September 09, 2011 9:34 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] I'm so sad for AFIP to close > >I never really thought it would happen but it has... > >http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat >hology-Community.html > > > >Joyce Weems >Pathology Manager >Saint Joseph's Hospital >5665 Peachtree Dunwoody Rd NE >Atlanta, GA 30342 >678-843-7376 - Phone >678-843-7831 - Fax > > >Confidentiality Notice: >This e-mail, including any attachments is the >property of Catholic Health East and is intended >for the sole use of the intended recipient(s).? >It may contain information that is privileged and >confidential.? Any unauthorized review, use, >disclosure, or distribution is prohibited. If you are >not the intended recipient, please delete this message, and >reply to the sender regarding the error in a separate email. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cedarwoman <@t> comcast.net Fri Sep 9 22:04:54 2011 From: cedarwoman <@t> comcast.net (cedarwoman@comcast.net) Date: Fri Sep 9 22:05:05 2011 Subject: [Histonet] AFIP closing Message-ID: <33465093.387303.1315623894300.JavaMail.root@sz0020a.emeryville.ca.mail.comcast.net> Hi Histonetters, especially Joyce as it seems we may be the 'same vintage' - but I have to chime in as an old-timer and say how sad I am too about the AFIP.? My histology career essentially started there. I was fortunate enough to have been trained there in Lee Luna's time, and knew the folks who literally "wrote the book" (you all know which one I mean, I'm sure, the Histo Bible). I was also at Walter Reed hospital - which also closed just in the past few days - which was then housed within the AFIP building, on the same floor actually. It sure makes me feel both blessed for such excellent training and at the same time "outmoded" for these institutions to be gone. There is little to add after that, but I enjoy reading the posts on Histonet just the same. Brigit in CA From SteveM <@t> mcclainlab.com Sat Sep 10 07:08:59 2011 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Sat Sep 10 06:56:19 2011 Subject: [Histonet] Almost Eliminating Floaters Message-ID: MY APOLOGIES FOR NOT DELETING THE REST OF THE HISTONET EMAIL FROM MY PRIOR RESPONSE. I HAVE ADDED ADDITIONAL NOTES AT THE END.- STEVE Floaters on the water bath are generally not the problem, are usually identifiable, unlike the serious issue posed by floaters in the block. I would suggest counting floaters to get a benchmark- ANY COUNT YOU MAKE WILL PROBABLY BE AN UNDERESTIMATE, BUT AT LEAST YOU HAVE SOMETHING TO TRACK.. In order to change the outcome, you (the institutional you) must change what you are doing. SO change your grossing-procedure. Start by scrupulously cleaning every pair of forceps. Keep an open vial of 50% alcohol at the bench to have the grossers rinse their forceps between specimens. Photograph every specimen-this forces the grossers to maintain a clean background. If you do not have grossing cameras, then mount a video camera above the grossing station and let it run. Point is a few hours study of technique can help identify where the problem is. Better than wasting many hours. Have grossers begin to wrap every small specimen in lens paper- Three darn good reasons. Wrapping specimens with forceps helps to clean the forceps before the next specimen. At embedding , the action of opening/unwrapping specimens with forceps cleans forceps at the embedding center. Floaters can also occur during processing-wrapping specimens prevents floaters during processing and keeps processors clean. (If you don't believe me- filter your first reagent when changing and then process that to a block- I once inherited a processor from a lab that did placentas. We found pieces of placenta in our derm specimens as long as 5 or 6 months later.) Change fixative and clean block bucket at the grossing bench routinely. You may wish to consider rinsing all blocks in fresh reagent BEFORE placing on processor. Evolving to this procedure, the floaters fell to 1 per 15,000, about 3 per year in our small lab. ADDITIONAL NOTES: Focusing on grossing is neither putting all your eggs in one basket nor blaming the grossers. Focusing on grossing floaters is focusing on the complex, dirty workplace where historically the vast majority (70-80-90%) of these errors are generated. The people may be part of the problem, yet the system is at the heart of the issue and determines the error-rate. The system is complicated, but includes the environment and structure and policy. There is too much going on at or near the grossing bench, make it quiet and simple, reduce distractions There are generally more grossers than embedders and greater variation in technique and sample size-(embedders generally only have to deal with samples 15x15mm or less.) And greater numbers of reagents and fixatives And perhaps even a cryostat for FS Or FAXITRON. Or a computer Accessioning, scanning documents and cassette printing Maybe a telephone. Or a reagent recycler Or a processor (eliminate as many distractions as you can. Separate quiet enclosed areas where specimens can be controlled and studied without distraction. For example we banned cellphones from the grossing area). Point is simplify and reduce noise. Make the grossing area for small specimens quiet. The singularly important reason we never adopted voice recognition in the grossing area had to do with the distractions imposed by messing with computers, the noise of talking into a machine, the distraction of looking at a screen to proofread while working with a one of a kind valuable specimen in the most error-prone area of the lab. Talking and looking at a computer screen is neither studying tissue nor professional. If you use that type of system, consider modifying your procedure to focus on getting the tissue cleanly in the block and the block safely in reagent, then do the dictation. Some of our grossers were resistant to change with valid reasons for doing so we let them be the baseline control- when we wanted to make a change we compared method A to method B. When one method was statistically better we showed them the data. There is room for the inevitable disagreement- but study and get some data and restudy if needed. Personally, I want to see grosser focused on the tissue, studying tissue as a trained professional; Once the tissue is safely in the cassette and in the next reagent (or fixative or ) then the grossers enter their own data. Scan the next bottle barcode and move on to the next specimen. 'Clean' is a relative term and a perceptual problem. (What is clean?-even when your grossing area is spotless-if one were to eat in the lab would you prefer to do so standing next to the grosser or the embedder? Not suggesting it is permitted- just which area is cleaner and what is meant by clean) SUGGESTIONS Have an EM (electron microscope) tech come in and get it clean to his/her satisfaction. They are used to clean-room mentality and standards Grossers are reluctant to change paper towels or other cutting surfaces or cutting tools between specimens. Make them freely available. Make the grossing benches spotless. Make the reagent buckets, spotless. Fresh reagents every shift- or whenever a speck appears. The instruments, spotless. Cut white or blue flat paper towels into 2.5x2.5 inch squares and gross on them- fewer floaters than brown C-fold towels or use pink dental wax sheets. Make a hard and fast rule about the number of tissue fragments per cassette, e.g., 5- not more. 5 pieces in a cassette can be seen and verified easily. Place similar sized pieces in cassettes (if there are 10 pieces, 5 each 1mm and 5 each 3mm- all the 1mm go into one cassette and all the 3 mm in the other cassette. A 1mm speck-floater is not obvious among 3mm pieces. A sixth 1mm speck in the 1mm cassette is noticeable. Use a separate area to gross large specimens and small biopsies, whether on different benches or at different times of day than the small biopsies. ADVANTAGES OF PHOTOGRAPHY(if one photographs every specimen as we do at McClain Labs and critiques grossers for knifemarks or bloody stains on the towel or leaving the same bloody background in consecutive photos by posting them in the lab or at staff meetings, they soon catch on. Rewards for great photographs also sends a message. Photography is a great method for verifying technique, for documenting your work, for ) METRICS- fewer is better. Track floaters by grosser to see if you have an outlier or risk-taker. Performance improves on any measured parameter when one follows through. Historically grossing performance has been measured by how fast the grosser completes x number of specimens. That metric should be suspended or abandoned if there are issues in the lab or you are working toward improving technique. FYI our other metrics are 1) lost specimens- (the number is zero in seven years) And 2) cumulative blocks since last maintenance for each processor. 3) catastrophic tissue loss- sounds a bit extreme, but means any hole or tissue loss on the slide visible at low power (2x). Each such slide is photographed, investigated and discussed at staff meeting of grossers and histotechs and pathologists. PERSONNEL Training histotechs to gross has a variety of benefits. My good experience biases me -my best grossers have been histotechs They respect the difficulty in embedding and cutting and communicate well with other histotechs. My second best are pathologists (I am the slowest) Physician assistants and residents are difficult (or maybe I demand too much). Both operate largely outside traditional lab feedback loops. (residents may be castigated by the attending who is legally responsible for their mistakes at the microscope and feel discomfort, yet embedding and cutting their mistakes at the microtome falls to the histotechs) I'd rather gross myself than to clean up messes made by residents. I was a jerk resident myself 1000 years ago- you know the trainee who is told to keep the blocks postage stamp-size and brings in a commemorative stamp? Eliminating floaters is analogous to troubleshooting staining problems where errors in technique in the early steps cause great variation in the final product. Begin by eliminating early points of failure (for staining-first care in handling prior to fixation, followed by prompt fixation, adequate fixation, then consistent thickness in grossing, then processing, then cutting). Errors in early steps cannot be fixed later on. Mashing tissue or prolonged drying or inadequate fixation cannot be fixed by re-processing. Once the floater is in the block, it is there to stay and proof by testing the DNA of the floater and patient is expensive, used to be $3000-$5000. It is better and less expensive to eliminate these errors at the source. Focusing on grossing not only gets rid of the main source(s) of variation, it also serves notice to the embedders once "grossing floaters and "processing floaters" are eliminated embedding is next. Steve A. McClain, MD 631 361 4000 NY BAD (MY bad from NY) I apologize for replying with all the stuff still attached. From rsrichmond <@t> gmail.com Sat Sep 10 20:36:01 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Sep 10 20:36:07 2011 Subject: [Histonet] Re: I'm so sad for AFIP to close Message-ID: Dave Low notes: >>The Tri-Service Histopathology School moved to Ft. Sam Houston [in San Antonio, Texas].<< Never heard of it. Can anybody tell us more about it? Their graduates must get some excellent jobs when they got out of the service. Bob Richmond Samurai Pathologist Knoxville TN From lowman034 <@t> yahoo.com Sat Sep 10 20:55:04 2011 From: lowman034 <@t> yahoo.com (Dave Low) Date: Sat Sep 10 20:55:08 2011 Subject: [Histonet] Re: I'm so sad for AFIP to close In-Reply-To: References: Message-ID: <1315706104.66048.YahooMailNeo@web65907.mail.ac4.yahoo.com> Dr. Richmond, ? They don't have a link set up so I'll get on my buddy who is the instructor but the school is at: http://www.metc.mil/academics/ ? The school is primary for military personnel and they go through all the histology principles to include some on-the-job training.? ? Dave From: Bob Richmond To: histonet@lists.utsouthwestern.edu Sent: Saturday, September 10, 2011 9:36 PM Subject: [Histonet] Re: I'm so sad for AFIP to close Dave Low notes: >>The Tri-Service Histopathology School moved to Ft. Sam Houston [in San Antonio, Texas].<< Never heard of it. Can anybody tell us more about it? Their graduates must get some excellent jobs when they got out of the service. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shirin_ake <@t> yahoo.com Sun Sep 11 00:40:48 2011 From: shirin_ake <@t> yahoo.com (shirin kouhpayeh) Date: Sun Sep 11 00:40:54 2011 Subject: [Histonet] uPAR Message-ID: <1315719648.81909.YahooMailMobile@web161003.mail.bf1.yahoo.com> Dear histonetters I'm working on a surface marker, uPAR, on lymph node tissue. For setting up the IHC-p procedure, I've chosen the ductal carcinoma sample as positive control. I use the citrate buffer ph=6 as retrieval solution and TBS ph=7.6 for wash steps. different Ag retrieval systems and different Ab ( santa cruz biotech. ) dilutions have been tested s 2*30 minutes of heating in water bath 70 degree of cellicius, 5 minutes with 800 watt power in microwave oven following 15 minutes heating with power of 300 6 minutes with max power ( 800 watt) following by 15 minutes on power 300 watt 10 minutes with max power following with 15 minutes on power 300 watt 20 min autoclave on 121 degree of cellicius But there is still no signal on ductal carcinoma sample. Is there anyone have a comment for me? I would be so appreciated . Shirin kouhpayeh Ph.D student Immunology dept. Isfahan university of medical science Isfahan, Iran From tshrobertson <@t> yahoo.com Sun Sep 11 01:03:23 2011 From: tshrobertson <@t> yahoo.com (Teisha Robertson) Date: Sun Sep 11 01:03:27 2011 Subject: [Histonet] (no subject) Message-ID: <1315721003.31338.YahooMailMobile@web126002.mail.ne1.yahoo.com> http://ofimix.com/img/banner_superior/hsptlfp.htm From amario3 <@t> uic.edu Sun Sep 11 12:46:07 2011 From: amario3 <@t> uic.edu (Andrea Marion) Date: Sun Sep 11 12:46:11 2011 Subject: [Histonet] uPAR Message-ID: Hi Shirin, It looks like you've tried a number of different heat intensities. Rather than try different combinations of heating, I think you would do better to try a different retrieval buffer entirely. Have you tried sodium citrate at pH 2.5, or 8.0? What about EDTA or enzymatic digestion? Different epitopes require different conditions. I haven't used the antibody you're referring to, but a quick search of google turned up a paper that tested a panel of different antibodies for uPAR against different antigen retrieval methods. It looks like they get the best results using pH 2.5 0.05 M sodium citrate, and heating to boiling in a microwave oven, then heating at 150W for 10 or 20 minutes. Pronase digestion gave better results. You can read the paper (free access) here: http://jhc.sagepub.com/content/46/4/469.full It might also be worth considering whether your positive control tissue was processed properly, and whether the antibodies you're using have been validated on FFPE tissues. Good luck, Andrea Andrea Marion Graduate Student University of Illinois at Chicago Dear histonetters I'm working on a surface marker, uPAR, on lymph node tissue. For setting up the IHC-p procedure, I've chosen the ductal carcinoma sample as positive control. I use the citrate buffer ph=6 as retrieval solution and TBS ph=7.6 for wash steps. different Ag retrieval systems and different Ab ( santa cruz biotech. ) dilutions have been tested s 2*30 minutes of heating in water bath 70 degree of cellicius, 5 minutes with 800 watt power in microwave oven following 15 minutes heating with power of 300 6 minutes with max power ( 800 watt) following by 15 minutes on power 300 watt 10 minutes with max power following with 15 minutes on power 300 watt 20 min autoclave on 121 degree of cellicius But there is still no signal on ductal carcinoma sample. Is there anyone have a comment for me? I would be so appreciated . Shirin kouhpayeh Ph.D student Immunology dept. Isfahan university of medical science Isfahan, Iran From jnocito <@t> satx.rr.com Sun Sep 11 16:27:02 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Sep 11 16:29:16 2011 Subject: [Histonet] AFIP closing In-Reply-To: <33465093.387303.1315623894300.JavaMail.root@sz0020a.emeryville.ca.mail.comcast.net> References: <33465093.387303.1315623894300.JavaMail.root@sz0020a.emeryville.ca.mail.comcast.net> Message-ID: I was stationed at AFIP from 1986-91. I was in the Immuno lab. I thought I was doing "imunos" when I was stationed in San Antonio, TX (remember the Dako kits?) I wasn't even close to the things that AFIP was doing. We were making our own rabbit antibodies. Made up our own link and label from scratch. Our DAB was order in 5gm powder bottles and we had to prep it under the fume hood in full PPE. I really learned about immunos there. Even tried a new method using a lead-based solution in a microwave to open up binding sites in FFPE tissue. Worked with a new automated immuno stainer from Fisher. Think it was called the "Brigoti" machine. I learned a lot there, but was ecstatic to return to San Antonio. Joe ----- Original Message ----- From: To: Sent: Friday, September 09, 2011 10:04 PM Subject: [Histonet] AFIP closing Hi Histonetters, especially Joyce as it seems we may be the 'same vintage' - but I have to chime in as an old-timer and say how sad I am too about the AFIP. My histology career essentially started there. I was fortunate enough to have been trained there in Lee Luna's time, and knew the folks who literally "wrote the book" (you all know which one I mean, I'm sure, the Histo Bible). I was also at Walter Reed hospital - which also closed just in the past few days - which was then housed within the AFIP building, on the same floor actually. It sure makes me feel both blessed for such excellent training and at the same time "outmoded" for these institutions to be gone. There is little to add after that, but I enjoy reading the posts on Histonet just the same. Brigit in CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From zulu1250 <@t> gmail.com Sun Sep 11 16:58:29 2011 From: zulu1250 <@t> gmail.com (Rocky J Squirrel) Date: Sun Sep 11 16:58:34 2011 Subject: [Histonet] AFIP Message-ID: AFIP has been transformed into JPC (Joint Pathology Commission). Histology School is now in Fort Huston (Texas) and the AFIP Histology lab is now part of Bethesda Nanal Medical Center Anatomic Pathology Department. Lloyd Dallas Retired Histology Technician From klakhman <@t> genomeweb.com Sun Sep 11 17:29:56 2011 From: klakhman <@t> genomeweb.com (Kirell Lakhman) Date: Sun Sep 11 17:30:03 2011 Subject: [Histonet] AFIP In-Reply-To: References: Message-ID: FYI: http://www.genomeweb.com/blog/ascp-mourns-closure-armed-forces-institute-pathology On Sun, Sep 11, 2011 at 5:58 PM, Rocky J Squirrel wrote: > AFIP has been transformed into JPC (Joint Pathology Commission). Histology > School is now in Fort Huston (Texas) and the AFIP Histology lab is now > part > of Bethesda Nanal Medical Center Anatomic Pathology Department. > > Lloyd Dallas > Retired Histology Technician > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Kirell Lakhman Editor, *The Sample* News editor, *GenomeWeb News* www.genomeweb.com +1 607-353-7102 Office +1 607-353-9310 Mobile ------------------------------------------------------- *The GenomeWeb Intelligence Network* From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Sep 12 07:35:00 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Sep 12 07:35:24 2011 Subject: [Histonet] RE: MYOD1 In-Reply-To: <6CB019913E5F6940AD6E4871841EC5364B5828@MBX-2.biosvr1.bioreference.com> References: <6CB019913E5F6940AD6E4871841EC5364B5828@MBX-2.biosvr1.bioreference.com> Message-ID: What platform are you using and from what company is your myoD1 Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna Liaros [dliaros@BioReference.com] Sent: Friday, September 09, 2011 4:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MYOD1 Any protocol recommendations for MYOD1? I am having a difficult time in trying to work up this antibody. The information transmitted in this email and any of its attachments is intended only for the person or entity to which it is addressed and may contain BioReference Laboratories proprietary information, which is privileged, confidential, or subject to copyright belonging to BioReference Laboratories. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited and may be unlawful. If you received this in error, please contact the sender immediately and delete and destroy the communication and all of the attachments you have received and all copies thereof. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mturner <@t> CSILaboratories.com Mon Sep 12 07:39:56 2011 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Mon Sep 12 07:40:59 2011 Subject: [Histonet] AFIP closing In-Reply-To: <33465093.387303.1315623894300.JavaMail.root@sz0020a.emeryville.ca.mail.comcast.net> References: <33465093.387303.1315623894300.JavaMail.root@sz0020a.emeryville.ca.mail.comcast.net> Message-ID: <28FD5AB4A24C8249BDBED1C7FA7C999A0314F9F5@csi-srv-007.CSI-LABS.local> Ah, Lee Luna. Now there was an inspirational person if there ever was one. His life story should be made into a movie. I attended an IHC workshop with him in Williamsburg, Virginia in the early 80's and we all went to dinner after the day's training session. As we waited for our food, he spoke and told us we each get one histology/IHC related question, and then we would enjoy the rest of the evening as friends. As we went around the table, the questions ranged from very basic to deep and intense, but he had an answer for everyone. Once that was over, we spent the rest of the evening getting to know each other. He was a great man! Mark Turner, HT(ASCP) QIHC CSI Laboratories Alpharetta, GA -----Original Message----- From: cedarwoman@comcast.net [mailto:cedarwoman@comcast.net] Sent: Friday, September 09, 2011 11:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFIP closing Hi Histonetters, especially Joyce as it seems we may be the 'same vintage' - but I have to chime in as an old-timer and say how sad I am too about the AFIP.? My histology career essentially started there. I was fortunate enough to have been trained there in Lee Luna's time, and knew the folks who literally "wrote the book" (you all know which one I mean, I'm sure, the Histo Bible). I was also at Walter Reed hospital - which also closed just in the past few days - which was then housed within the AFIP building, on the same floor actually. It sure makes me feel both blessed for such excellent training and at the same time "outmoded" for these institutions to be gone. There is little to add after that, but I enjoy reading the posts on Histonet just the same. Brigit in CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicole <@t> dlcjax.com Mon Sep 12 08:08:38 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Mon Sep 12 08:11:37 2011 Subject: [Histonet] Histo-ville Message-ID: <3423.208.62.167.196.1315832918.squirrel@webmail.realpages.com> Thank you for joining the facebook group Histo-ville. I have already meet some great people. I hope more of you will join and use the facebook site as a way to communicate, make friends, and troubleshoot. I hope you meet you soon, Nicole Tatum, HT ASCP From Erin.Martin <@t> ucsf.edu Mon Sep 12 09:46:49 2011 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Mon Sep 12 09:46:54 2011 Subject: [Histonet] Microm 355S Message-ID: <5FBE47EA8AF0244C977FD921277FA2B548D4462396@EX06.net.ucsf.edu> Good morning! We are having a lot of trouble with one of our Microm 355S microtomes. It advances forward at random times so that it ends up chunking into the block. The first time we had a repair person in, he was skeptical that it was moving on its own - until it did it to him! Since then, we have had a repair people in several times (under service contract, thank goodness). One told me that random block advancement is a common problem with this model. He also said that it is very difficult to find the cause and is therefore very hard fix it. We've replaced fuses, circuit boards... and it's still doing it. Has anyone else seen this problem and if so how did they fix it? Thanks in advance! Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 From Ashley.Troutman <@t> Vanderbilt.Edu Mon Sep 12 09:49:23 2011 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Mon Sep 12 09:50:00 2011 Subject: [Histonet] MYOD1 Message-ID: <7B310892042DA74CB3590053F424CFE61441FCEE28@ITS-HCWNEM06.ds.Vanderbilt.edu> Hi Donna, I had a time with this one as well. I use Dako's antibody on the Leica Bondmax. I run it at 1:50 using ER2 for 30 minutes. I would be careful going any stronger on the dilution, I got quite a bit of background with a 1:40, the 1:50 stains clean but can be a little weak if you don't use a fresh cut slide. I ultimately decided to go with the 1:50 because I could get cleaner, consistent staining with it. An equivalent for this protocol would be an EDTA (High pH) retrieval in a pressure cooker for about 20 minutes with a 10 minute cool down. Antibody incubation on the Bond protocol is 15 minutes. I would recommend about 30 minutes on an open system or if you are doing it by hand. If you are running this on a Ventana platform I would start with a CC1 standard and at least 40 minutes on the antibody incubation. You may have to bump the retrieval time up to the extended. Good luck! Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 From settembr <@t> umdnj.edu Mon Sep 12 10:17:44 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Mon Sep 12 10:17:58 2011 Subject: [Histonet] RE: MYO-D1 In-Reply-To: <6CB019913E5F6940AD6E4871841EC5364B5828@MBX-2.biosvr1.bioreference.com> References: <6CB019913E5F6940AD6E4871841EC5364B5828@MBX-2.biosvr1.bioreference.com> Message-ID: Hi Donna, I use Dako's Myo-D1 cat. M3512. Use it at 1:25 I use Dako's labeled polymer kit, Envision + Mouse. Use Trilogy (from Cell Marque) in a steamer. That means: Start the steamer and put a two Coplin jars in filled with Trilogy. Place all your slides in 1st Coplin jar for 30 min. right then, at RT. The temp will go up to 95 degrees as the Trilogy acts as a deparaffinizer. Then put he slides in the 2nd Coplin jar for 30 min. and Trilogy acts as AR. Good luck. Dana Settembre University Hospital - UMDNJ Newark, NJ 07103 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna Liaros Sent: Friday, September 09, 2011 4:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MYOD1 Any protocol recommendations for MYOD1? I am having a difficult time in trying to work up this antibody. The information transmitted in this email and any of its attachments is intended only for the person or entity to which it is addressed and may contain BioReference Laboratories proprietary information, which is privileged, confidential, or subject to copyright belonging to BioReference Laboratories. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited and may be unlawful. If you received this in error, please contact the sender immediately and delete and destroy the communication and all of the attachments you have received and all copies thereof. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From turkekul <@t> gmail.com Mon Sep 12 12:14:07 2011 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Mon Sep 12 12:14:13 2011 Subject: [Histonet] TUNEL on Ventana Message-ID: Hi Guys, Has anyone of you done TUNEL on Ventana? Mesruh Turkekul mskcc.org From HParker <@t> Skaggs.Net Mon Sep 12 12:13:50 2011 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Mon Sep 12 12:17:10 2011 Subject: [Histonet] AFIP Closing In-Reply-To: <7fd1bbf0-7ccd-4e5d-9af6-f4bf9a66f824@email1.skaggs.net> References: <7fd1bbf0-7ccd-4e5d-9af6-f4bf9a66f824@email1.skaggs.net> Message-ID: <930EB2E8DF68C544873EDD2A3D5F50600588FF24AE@email1.skaggs.net> Sad that is where I went thru Histology Training at the Tri-Service School of Histotechnology in 1985. I was in a class of 10 Air Force guys and I was Navy and there was one other Navy guy. I was the only female in my class. It was interesting and have been doing Histology non-stop since then- wow 25+ years has gone by fast. Helayne Parker, H.T. (ASCP) Pathology Section Head Skaggs Regional Medical Center The Best Place to Get Better P.O. Box 650, Branson Missouri 65615 Direct: 417-335-7254 Fax: 417-335-7127 E-Mail: hparker@skaggs.net Web: www.skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. From tpodawiltz <@t> lrgh.org Mon Sep 12 12:31:47 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Sep 12 12:32:00 2011 Subject: [Histonet] RE: AFIP Closing In-Reply-To: <930EB2E8DF68C544873EDD2A3D5F50600588FF24AE@email1.skaggs.net> References: <7fd1bbf0-7ccd-4e5d-9af6-f4bf9a66f824@email1.skaggs.net> <930EB2E8DF68C544873EDD2A3D5F50600588FF24AE@email1.skaggs.net> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DF21A15F@LRGHEXVS1.practice.lrgh.org> While I was in the Navy 77-83, I spent a week at AFIP for special stain course they had. I got to meet Lee Luna, but did not get to spend a lot of time with the man. However, my most memorable trip to AFIP was when I was stationed at the National Naval Medical Center in Bethesda, Maryland (the old center). I was sent to AFIP with a box of specimens to deliver to one of the labs and my duty driver got lost. I had read the manifest of the contents and when the driver asked me if I had any idea of where were, I said "No" and then followed up with this statement. "You mean to tell me that we have five brains in this car and we can't figure out where we are?" Yep, I was delivering three brains from an air crash accident for study. My duty driver could not wait to get rid of the box. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Monday, September 12, 2011 1:14 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] AFIP Closing Sad that is where I went thru Histology Training at the Tri-Service School of Histotechnology in 1985. I was in a class of 10 Air Force guys and I was Navy and there was one other Navy guy. I was the only female in my class. It was interesting and have been doing Histology non-stop since then- wow 25+ years has gone by fast. Helayne Parker, H.T. (ASCP) Pathology Section Head Skaggs Regional Medical Center The Best Place to Get Better P.O. Box 650, Branson Missouri 65615 Direct: 417-335-7254 Fax: 417-335-7127 E-Mail: hparker@skaggs.net Web: www.skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Randi.Hayes <@t> horizonnb.ca Mon Sep 12 12:40:45 2011 From: Randi.Hayes <@t> horizonnb.ca (Hayes, Randi (HorizonNB)) Date: Mon Sep 12 12:40:51 2011 Subject: [Histonet] Spirochete Sensitizer Message-ID: Hi everyone, I'm wondering what people are using as a Spirochete Sensitizer. It's been suggested to us to use a 1% Uranyl Nitrate solution but we're a little wary of making our own solution (nasty stuff!). Does anyone know if someone sells this commercially, not in a kit? If not, is there anything else that has proven to work well? Thanks, Randi Hayes Histology Supervisor Horizon Health Network Moncton, NB Canada ------- Horizon Health Network Disclaimer -------

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From jqb7 <@t> cdc.gov  Mon Sep 12 12:52:05 2011
From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID))
Date: Mon Sep 12 12:52:10 2011
Subject: [Histonet] RE: Spirochete Sensitizer
In-Reply-To: 
References: 
Message-ID: 

When you receive the product insert you will find that this solution is quite safe.


www.newcomersupply.com

NITRATE 1% Aqueous Solution
(use: Steiner Silver Impregnation Methods.)  
 
250 ml 14036A $82.90
500 ml 14036B $160.10
1 liter 14036C $315.70

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hayes, Randi (HorizonNB)
Sent: Monday, September 12, 2011 1:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Spirochete Sensitizer


Hi everyone,

I'm wondering what people are using as a Spirochete Sensitizer.  It's been suggested to us to use a 1% Uranyl Nitrate solution but we're a little wary of making our own solution (nasty stuff!).  Does anyone know if someone sells this commercially, not in a kit?  If not, is there anything else that has proven to work well?

Thanks,

Randi Hayes
Histology Supervisor
Horizon Health Network
Moncton, NB Canada
------- Horizon Health Network Disclaimer -------

This e-mail communication (including any or all attachments) is intended
only for the use of the person or entity to which it is addressed and may
contain confidential and/or privileged material. If you are not the intended
recipient of this e-mail, any use, review, retransmission, distribution,
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reliance upon this e-mail, is strictly prohibited. If you have received this
e-mail in error, please contact the sender and delete the original and any
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co-operation is appreciated.

Le pr?sent courriel (y compris toute pi?ce jointe) s'adresse uniquement ?
son destinataire, qu'il soit une personne ou un organisme, et pourrait
comporter des renseignements privil?gi?s ou confidentiels. Si vous n'?tes
pas le destinataire du courriel, il est interdit d'utiliser, de revoir, de
retransmettre, de distribuer, de diss?miner, de copier ou d'imprimer ce
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reconnaissants de votre collaboration.
From Stacy_McLaughlin <@t> cooley-dickinson.org  Mon Sep 12 14:12:30 2011
From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin)
Date: Mon Sep 12 14:13:20 2011
Subject: [Histonet] CAP: antibody and reagent lot validation
Message-ID: 

Hello in Histoland!

Working on our SOP for new antibody validation, new lot validation of
antibodies and detection kits.

Is there anyone willing to share info?

Do you list specific #'s of tissues/cases in your SOP's that you use to
validate?  Do you use a multi-tissue block for all of your antibodies,
or do you have a multi-tissue block for certain markers?

 

Are there any resources you know of out there that can help me?

 

Thanks in advance for sharing your wealth of knowledge!

 

 

 

Stacy McLaughlin, HT(ASCP)

Histology Supervisor

Cooley Dickinson Hospital

30 Locust Street

Northampton, MA 01060

Stacy_McLaughlin@Cooley-Dickinson.org

 

From MHillmer <@t> dermwisconsin.com  Mon Sep 12 14:31:12 2011
From: MHillmer <@t> dermwisconsin.com (Michael Hillmer)
Date: Mon Sep 12 14:31:17 2011
Subject: [Histonet] Job Opening
In-Reply-To: 
References: 
Message-ID: 


We are looking for a certified HT or HTL, or applicants eligible to sit for the certification exam, to join our pathology lab in Manitowoc, WI.  We currently have a fantastic 1st shift position open.  We have excellent benefits, state-of-the-are lab (less than a year old) and a great team to work with.  We will assist with relocation.  Please contact me if you have any questions.

Michael Hillmer PHR
HR Coordinator
Dermatology Associates of Wisconsin
Phone: (920)683-5278
Fax: (920)686-9674
Cell: (920)860-6360

The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule.  If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law.  If you have received this e-mail in error, please notify me by reply e-mail and then delete this message.  Do not pass any of this information to anyone else.  Thank you for your cooperation.

________________________________

The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation.
From jshelley <@t> sanfordburnham.org  Mon Sep 12 14:58:30 2011
From: jshelley <@t> sanfordburnham.org (John Shelley)
Date: Mon Sep 12 14:58:35 2011
Subject: [Histonet] RE: Microm 355S
In-Reply-To: <5FBE47EA8AF0244C977FD921277FA2B548D4462396@EX06.net.ucsf.edu>
References: <5FBE47EA8AF0244C977FD921277FA2B548D4462396@EX06.net.ucsf.edu>
Message-ID: <5A605CE38EECB64B94485C02125A0C440708D092@LN-MAIL07.ln.burnham.org>

Hi Erin,

I have experienced exactly what you described. I have had it chunk a block a few times but also have come to the unit after cutting and the holder has advanced all the way to its end point. Very disturbing, but I worked around that by hitting the stop button when I am done so that does not happen and in regards to the advancing with a block I just take my time and I find when I hit the button on the side that is used for forward or backward advancement it appears to correct the problem. This is why I much prefer Leica brand microtomes but this was here before I started. Good Luck!!!

Kind Regards!
?
John J Shelley
Senior Research Associate, Histology Core Facility
Sanford-Burnham Medical Research Institute at Lake Nona
6400 Sanger Road??????????????????????????????? 
Orlando, FL 32827??????????????????????????????????? 
Tel: (407) 745-2000 Ext.2517
Fax: (407) 745-2001
email:  jshelley@sanfordburnham.org



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Monday, September 12, 2011 10:47 AM
To: histonet
Subject: [Histonet] Microm 355S

Good morning!

We are having a lot of trouble with one of our Microm 355S microtomes.  It advances forward at random times so that it ends up chunking into the block.  The first time we had a repair person in, he was skeptical that it was moving on its own - until it did it to him!  Since then, we have had a repair people in several times (under service contract, thank goodness).  One told me that random block advancement is a common problem with this model.  He also said that it is very difficult to find the cause and is therefore very hard fix it.  We've replaced fuses, circuit boards...  and it's still doing it.  Has anyone else seen this problem and if so how did they fix it?

Thanks in advance!
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From billodonnell <@t> catholichealth.net  Mon Sep 12 15:29:49 2011
From: billodonnell <@t> catholichealth.net (O'Donnell, Bill)
Date: Mon Sep 12 15:30:06 2011
Subject: [Histonet] RE: AFIP Closing
In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DF21A15F@LRGHEXVS1.practice.lrgh.org>
References: <7fd1bbf0-7ccd-4e5d-9af6-f4bf9a66f824@email1.skaggs.net><930EB2E8DF68C544873EDD2A3D5F50600588FF24AE@email1.skaggs.net>
	<38667E7FB77ECD4E91BFAEB8D986386323DF21A15F@LRGHEXVS1.practice.lrgh.org>
Message-ID: <4940DF6D1C5FDF48931B6966AAEF93951F2161@chimsx08.CHI.catholichealth.net>

Tom, That's great. I had never heard that one. - Bill
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Podawiltz, Thomas
Sent: Monday, September 12, 2011 12:32 PM
To: Parker, Helayne; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: AFIP Closing

While I was in the Navy 77-83, I spent a week at AFIP for special stain
course they had. I got to meet Lee Luna, but did not get to spend a lot
of time with the man. However, my most memorable trip to AFIP was when I
was stationed at the National Naval Medical Center in Bethesda, Maryland
(the old center). 
I was sent to AFIP with a box of specimens to deliver to one of the labs
and my duty driver got lost. I had read the manifest of the contents and
when the driver asked me if I had any idea of where were, I said "No"
and then followed up with this statement. "You mean to tell me that we
have five brains in this car and we can't figure out where we are?" Yep,
I was delivering three brains from an air crash accident for study. My
duty driver could not wait to get rid of the box. 


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220

Tom

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker,
Helayne
Sent: Monday, September 12, 2011 1:14 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] AFIP Closing

 Sad that is where I went thru Histology Training at the Tri-Service
School of Histotechnology in 1985.  I was in a class of 10 Air Force
guys and I was Navy and there was one other Navy guy.  I was the only
female in my class.  It was interesting and have been doing Histology
non-stop since then- wow 25+ years has gone by fast.  


Helayne Parker, H.T. (ASCP)
Pathology Section Head

Skaggs Regional Medical Center
The Best Place to Get Better

P.O. Box 650, Branson Missouri 65615
Direct: 417-335-7254
Fax: 417-335-7127
E-Mail: hparker@skaggs.net
Web: www.skaggs.net
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From leah.simmons <@t> industry.nsw.gov.au  Mon Sep 12 19:43:36 2011
From: leah.simmons <@t> industry.nsw.gov.au (leah.simmons@industry.nsw.gov.au)
Date: Mon Sep 12 19:45:31 2011
Subject: [Histonet] Where to start? Neospora and Toxoplasma
	Immunohistochemistry
Message-ID: 

Hi Everyone

I was hoping someone might be able to give me some advice on introducing 
Immunohistochemistry into our Laboratory.

We would like to start initially with Neospora in Cattle and Toxoplasma in 
Sheep.

Is anyone performing these and willing to help me out?

Thank you

Leah Simmons

Industry and Investment NSW
Elizabeth Macarthur Agricultural Institute

State  Veterinary Diagnostic Laboratory
Histology
Technical Officer
02 4640 6422
This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of their organisation.

From wanglianebox <@t> yahoo.com.cn  Tue Sep 13 04:09:23 2011
From: wanglianebox <@t> yahoo.com.cn (=?utf-8?B?6L+eIOeOiw==?=)
Date: Tue Sep 13 04:10:26 2011
Subject: [Histonet] Ask for techenical help - about sections of tiny tissue.
Message-ID: <1315904963.54446.YahooMailClassic@web15707.mail.cnb.yahoo.com>











Hello every Histonet - I could really need your help!
?
Our lab is currently obsevring pathlogical changes of aortic disease, especially 
elastic fiber and collagen in the background of abdominal aortic aneurysm (AAA). 
The aortic is from C57BL/6N mice.

First, I paid attention to the normal morphology of aortic artery.
But now the big problem is that the structure (mainly collagen and 
connective tissue) around the aortic artery couldn't be presevered. 
It seems to be destroyed, either in normal (with no stimulus) or AAA mice.
See my photo link:
? FIG 1 http://b212.photo.store.qq.com/psu?/6ebc69b4-17f2-43a6-8a04-6d96e913236e/ZMIqx1ix346XZIyxS1VvSWzGFiZVqs6Zq1x0C88Zzm4!/b/YQVfan6ScQAAYknHYn4wcQAA 
? FIG?2 http://b213.photo.store.qq.com/psu?/6ebc69b4-17f2-43a6-8a04-6d96e913236e/DAxmNEc.gpYU0RrrHx.IbhhjbH95LeEISm4c0BrlACM!/b/Ya5d.35gGwAAYriHBH.tGwAA 
? FIG?3 http://b212.photo.store.qq.com/psu?/6ebc69b4-17f2-43a6-8a04-6d96e913236e/4o43xMTRh6HzNzWacHUSmWN*4ZhH5.Y6RrqQl5SX7uI!/b/YcgpXn7ZcgAAYqHJYn5JcgAA 
? FIG?4 http://b212.photo.store.qq.com/psu?/6ebc69b4-17f2-43a6-8a04-6d96e913236e/sj62yPO5AmwjtA42o6asO11HxlKF4DQ8SyWMlTypyoc!/b/YYP0a36vcAAAYo1IYX4WcQAA 
?
I reviewed the orginal papers published in recent 10 years, and I am sure in 
our study conditions, these structures around the aortic ring should be intact.
?
See figure link(reprint from Ann Surg 2005;241: 92?101 and Atherosclerosis 2011;217: 350-357)
http://b211.photo.store.qq.com/psu?/6ebc69b4-17f2-43a6-8a04-6d96e913236e/X5QltJZABnkSKByT7E2K9SAcqt97uWhfgZBrmsyZ9w0!/b/YevLzn37cQAAYiAkx312cAAA 
and 
http://b210.photo.store.qq.com/psu?/6ebc69b4-17f2-43a6-8a04-6d96e913236e/mmpt1J8*7L*r0g6TBdCMZ3hk3n6KrOc9mKvXYmm4JdE!/b/YUXEOn0KnwAAYhUHLX0WnAAA 
?
?
In a word, this destruction seems to be due to technical procedure, but not the study-design itself.
?
The main steps are as follows:
1. Specimens were cut from sacrificed mice by minimally invasive scissors. It is impossible to destroy normal structure.
2. Tissue specimens were aortic artery between the renal arteries and the iliac bifurcation, which is about 8mm long and the diameter is 0.55mm.
?? Given the tiny volumn, specimens were fixed by 10% neutral PBS-formalin for 2h. I have tried from 2h to 48h, the H&E stain result is similar.
3. Dehydration and clearing are automated by a mechanical processor. 
?? Then the tissue is embedded with paraffin.
4. Embedded tissues were cut into 2-6 um sections with a microtome by a skilled technician. He has tried 2-6 um, and the H&E stain result is similar.
?? Sections were floated on a room-temperature water bath, then transferred to a warm water bath (I have tried 40 or 50 degree centigrade bath, the H&E stain 
result is similar), then picked up on glass slides.

All procedures are performed in a Leica microsystem.

My questions are:
?? 1. Why the normal structure around aortic ring is destroyed?
?? 2. How to improve the procedure? 

Thank you in advance for all your suggestions!
?
Wang Lian, MD 
Medical School of Nanjing University
Hankou Road 22#
Nanjing, Jiangsu Province, China
e-mail: wanglianebox@yahoo.com.cn
From wim.vandenbroeck <@t> UGent.be  Tue Sep 13 07:08:53 2011
From: wim.vandenbroeck <@t> UGent.be (Prof. dr. Wim Van den Broeck)
Date: Tue Sep 13 07:09:02 2011
Subject: [Histonet] immunohistochemistry: manual staining
Message-ID: <004401cc720d$e801b8b0$b8052a10$@vandenbroeck@UGent.be>

Dear Colleagues,

We do a lot of immunohistochemical stainings (research, no diagnostics), but
unfortunately, we cannot afford an automated stainer. At the moment, we do
everything manually (washing, incubating, ....) but I would like to make
things run more smoothly. So my question is, is there any device or systems
that can be helpful during manual IHC stainings ? 

Thanks in advance for your comments / suggestions.

-- 
Met vriendelijke groeten,


Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM
wim.vandenbroeck@UGent.be - tel.: +32 9 264 77 16 - fax: +32 9 264 77 90


E-maildisclaimer





From nto <@t> stowers.org  Tue Sep 13 07:34:13 2011
From: nto <@t> stowers.org (Thomas, Nancy)
Date: Tue Sep 13 07:34:22 2011
Subject: [Histonet] immunohistochemistry: manual staining
In-Reply-To: <004401cc720d$e801b8b0$b8052a10$@vandenbroeck@UGent.be>
References: <004401cc720d$e801b8b0$b8052a10$@vandenbroeck@UGent.be>
Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9841B2F3D@EXCHMB-02.stowers-institute.org>

We use the Sequenza immunostaining center available through Thermo.  The slides are inserted upright, with an attached coverplate.  This keeps the slides from drying out and also reduces the amount of reagents needed.  

Nancy Thomas 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Prof. dr. Wim Van den Broeck
Sent: Tuesday, September 13, 2011 7:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] immunohistochemistry: manual staining

Dear Colleagues,

We do a lot of immunohistochemical stainings (research, no diagnostics), but unfortunately, we cannot afford an automated stainer. At the moment, we do everything manually (washing, incubating, ....) but I would like to make things run more smoothly. So my question is, is there any device or systems that can be helpful during manual IHC stainings ? 

Thanks in advance for your comments / suggestions.

--
Met vriendelijke groeten,


Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM
wim.vandenbroeck@UGent.be - tel.: +32 9 264 77 16 - fax: +32 9 264 77 90


E-maildisclaimer





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From jshelley <@t> sanfordburnham.org  Tue Sep 13 08:05:45 2011
From: jshelley <@t> sanfordburnham.org (John Shelley)
Date: Tue Sep 13 08:07:38 2011
Subject: [Histonet] immunohistochemistry: manual staining
In-Reply-To: <004401cc720d$e801b8b0$b8052a10$@vandenbroeck@UGent.be>
References: <004401cc720d$e801b8b0$b8052a10$@vandenbroeck@UGent.be>
Message-ID: <5A605CE38EECB64B94485C02125A0C440708D149@LN-MAIL07.ln.burnham.org>

I love to use the Thermo Shandon Sequenza system. You touch the slides one time as you place a cover-plate on them and then you just add your reagents at the top and let gravity and capillary effect do its job. If you would like more information just contact me directly. Thanks!!!

Kind Regards!
?
John J Shelley
Senior Research Associate, Histology Core Facility
Sanford-Burnham Medical Research Institute at Lake Nona

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Prof. dr. Wim Van den Broeck
Sent: Tuesday, September 13, 2011 8:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] immunohistochemistry: manual staining

Dear Colleagues,

We do a lot of immunohistochemical stainings (research, no diagnostics), but
unfortunately, we cannot afford an automated stainer. At the moment, we do
everything manually (washing, incubating, ....) but I would like to make
things run more smoothly. So my question is, is there any device or systems
that can be helpful during manual IHC stainings ? 

Thanks in advance for your comments / suggestions.

-- 
Met vriendelijke groeten,


Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM
wim.vandenbroeck@UGent.be - tel.: +32 9 264 77 16 - fax: +32 9 264 77 90


E-maildisclaimer





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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From contact <@t> excaliburpathology.com  Tue Sep 13 08:25:38 2011
From: contact <@t> excaliburpathology.com (Paula Pierce)
Date: Tue Sep 13 08:25:46 2011
Subject: [Histonet] immunohistochemistry: manual staining
In-Reply-To: <5A605CE38EECB64B94485C02125A0C440708D149@LN-MAIL07.ln.burnham.org>
References: <004401cc720d$e801b8b0$b8052a10$@vandenbroeck@UGent.be>
	<5A605CE38EECB64B94485C02125A0C440708D149@LN-MAIL07.ln.burnham.org>
Message-ID: <1315920338.63238.YahooMailRC@web1102.biz.mail.sk1.yahoo.com>

Ditto the Sequenza!!!!

I work almost exclusively with eyes and they wash off the slides easily. The 
washes of the automated machines are too rough for them. The Sequenza is all I 
use!!!
?
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com




________________________________
From: John Shelley 
To: "wim.vandenbroeck@UGent.be" ; 
"histonet@lists.utsouthwestern.edu" 
Sent: Tue, September 13, 2011 8:05:45 AM
Subject: RE: [Histonet] immunohistochemistry: manual staining

I love to use the Thermo Shandon Sequenza system. You touch the slides one time 
as you place a cover-plate on them and then you just add your reagents at the 
top and let gravity and capillary effect do its job. If you would like more 
information just contact me directly. Thanks!!!

Kind Regards!
?
John J Shelley
Senior Research Associate, Histology Core Facility
Sanford-Burnham Medical Research Institute at Lake Nona

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Prof. dr. Wim 
Van den Broeck
Sent: Tuesday, September 13, 2011 8:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] immunohistochemistry: manual staining

Dear Colleagues,

We do a lot of immunohistochemical stainings (research, no diagnostics), but
unfortunately, we cannot afford an automated stainer. At the moment, we do
everything manually (washing, incubating, ....) but I would like to make
things run more smoothly. So my question is, is there any device or systems
that can be helpful during manual IHC stainings ? 

Thanks in advance for your comments / suggestions.

-- 
Met vriendelijke groeten,


Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM
wim.vandenbroeck@UGent.be - tel.: +32 9 264 77 16 - fax: +32 9 264 77 90


E-maildisclaimer





_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From SWeaver <@t> tvmdl.tamu.edu  Tue Sep 13 08:35:39 2011
From: SWeaver <@t> tvmdl.tamu.edu (Weaver, Stephanie)
Date: Tue Sep 13 08:35:33 2011
Subject: [Histonet] embedding centers
Message-ID: 

Hello histonetters!

I know this has been asked before, but there's not much in the recent archives.  I'd like to see what everyone thinks is now the best paraffin embedding center.  They all seem very similar now, and I don't see any one instrument that looks much different from the others.  My primary concern is reliability and long working life, but of course I would also like an instrument that is user friendly, ergonomic and affordable.  Please let me know if you have a very good experience with any embedding center or especially if anyone has had a particularly bad experience and let me know any features that you find spectacular or useless.  Thanks for the advice!

Stephanie Weaver
Texas Veterinary Medical Diagnostic Laboratory

From jqb7 <@t> cdc.gov  Tue Sep 13 08:36:46 2011
From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID))
Date: Tue Sep 13 08:37:00 2011
Subject: [Histonet] RE: embedding centers
In-Reply-To: 
References: 
Message-ID: 

Sakura's Tissue-Tek

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weaver, Stephanie
Sent: Tuesday, September 13, 2011 9:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding centers

Hello histonetters!

I know this has been asked before, but there's not much in the recent archives.  I'd like to see what everyone thinks is now the best paraffin embedding center.  They all seem very similar now, and I don't see any one instrument that looks much different from the others.  My primary concern is reliability and long working life, but of course I would also like an instrument that is user friendly, ergonomic and affordable.  Please let me know if you have a very good experience with any embedding center or especially if anyone has had a particularly bad experience and let me know any features that you find spectacular or useless.  Thanks for the advice!

Stephanie Weaver
Texas Veterinary Medical Diagnostic Laboratory

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From MSHERWOOD <@t> PARTNERS.ORG  Tue Sep 13 08:40:11 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret)
Date: Tue Sep 13 08:40:19 2011
Subject: [Histonet] embedding centers
In-Reply-To: 
References: 
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB59C1@PHSXMB30.partners.org>

Ditto.  Sakura's Tissue-Tek 


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weaver,
Stephanie
Sent: Tuesday, September 13, 2011 9:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding centers

Hello histonetters!

I know this has been asked before, but there's not much in the recent archives.
I'd like to see what everyone thinks is now the best paraffin embedding center.
They all seem very similar now, and I don't see any one instrument that looks
much different from the others.  My primary concern is reliability and long
working life, but of course I would also like an instrument that is user
friendly, ergonomic and affordable.  Please let me know if you have a very good
experience with any embedding center or especially if anyone has had a
particularly bad experience and let me know any features that you find
spectacular or useless.  Thanks for the advice!

Stephanie Weaver
Texas Veterinary Medical Diagnostic Laboratory

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


From sbaldwin <@t> mhhcc.org  Tue Sep 13 09:00:45 2011
From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org)
Date: Tue Sep 13 09:00:54 2011
Subject: [Histonet] P63 CONTROLS
Message-ID: 

HEY THANKS HISTONETTEERS
For all your help on controls we have the Ventana benchmark LT,  ran the controls and they are not working maybe we need to tweek our protocol can anyone help with this ?

Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-996-0210, 0216,  Fax 812-996-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
From smcbride <@t> andrew.cmu.edu  Tue Sep 13 09:04:37 2011
From: smcbride <@t> andrew.cmu.edu (Sean McBride)
Date: Tue Sep 13 09:04:46 2011
Subject: [Histonet] embedding centers
In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB59C1@PHSXMB30.partners.org>
References: 
	<073AE2BEA1C2BA4A8837AB6C4B943D9708DB59C1@PHSXMB30.partners.org>
Message-ID: 

Double ditto.  Sakura TEC 

~Sean McBride


Scientific Specialist
Bone Tissue Engineering Center
Carnegie Mellon Research Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124

412-268-8275 (o)
412-915-1683 (m)
412-268-8275 (fax)
smcbride@andrew.cmu.edu 






On Sep 13, 2011, at 9:40 AM, Sherwood, Margaret wrote:

> Ditto.  Sakura's Tissue-Tek 
> 
> 
> Peggy Sherwood
> Lab Associate, Photopathology
> Wellman Center for Photomedicine (EDR 214)
> Massachusetts General Hospital
> 50 Blossom Street
> Boston, MA 02114-2696
> 617-724-4839 (voice mail)
> 617-726-6983 (lab)
> 617-726-1206 (fax)
> msherwood@partners.org
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weaver,
> Stephanie
> Sent: Tuesday, September 13, 2011 9:36 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] embedding centers
> 
> Hello histonetters!
> 
> I know this has been asked before, but there's not much in the recent archives.
> I'd like to see what everyone thinks is now the best paraffin embedding center.
> They all seem very similar now, and I don't see any one instrument that looks
> much different from the others.  My primary concern is reliability and long
> working life, but of course I would also like an instrument that is user
> friendly, ergonomic and affordable.  Please let me know if you have a very good
> experience with any embedding center or especially if anyone has had a
> particularly bad experience and let me know any features that you find
> spectacular or useless.  Thanks for the advice!
> 
> Stephanie Weaver
> Texas Veterinary Medical Diagnostic Laboratory
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> The information in this e-mail is intended only for the person to whom it is
> addressed. If you believe this e-mail was sent to you in error and the e-mail
> contains patient information, please contact the Partners Compliance HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in error
> but does not contain patient information, please contact the sender and properly
> dispose of the e-mail.
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From rjbuesa <@t> yahoo.com  Tue Sep 13 09:05:07 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Sep 13 09:05:16 2011
Subject: [Histonet] embedding centers
In-Reply-To: 
Message-ID: <1315922707.20044.YahooMailClassic@web65710.mail.ac4.yahoo.com>

As you correctly point out, all are of similar configuration and foot-print. All use the same technology (a cooling area, a heating bath and a hot?compartment).
As to reliability TissueTek if the best for me. There are some more than 25 years old still?providing a good and reliable service.
Ren? J.

--- On Tue, 9/13/11, Weaver, Stephanie  wrote:


From: Weaver, Stephanie 
Subject: [Histonet] embedding centers
To: "histonet@lists.utsouthwestern.edu" 
Date: Tuesday, September 13, 2011, 9:35 AM


Hello histonetters!

I know this has been asked before, but there's not much in the recent archives.? I'd like to see what everyone thinks is now the best paraffin embedding center.? They all seem very similar now, and I don't see any one instrument that looks much different from the others.? My primary concern is reliability and long working life, but of course I would also like an instrument that is user friendly, ergonomic and affordable.? Please let me know if you have a very good experience with any embedding center or especially if anyone has had a particularly bad experience and let me know any features that you find spectacular or useless.? Thanks for the advice!

Stephanie Weaver
Texas Veterinary Medical Diagnostic Laboratory

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From jqb7 <@t> cdc.gov  Tue Sep 13 09:08:03 2011
From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID))
Date: Tue Sep 13 09:08:13 2011
Subject: [Histonet] embedding centers
In-Reply-To: <1315922707.20044.YahooMailClassic@web65710.mail.ac4.yahoo.com>
References: 
	<1315922707.20044.YahooMailClassic@web65710.mail.ac4.yahoo.com>
Message-ID: 

Honestly, there are differences ergonomically.  We purchased one in the past before realizing that the way the cassette holding bath was situated you had to pull the cassettes across the cold plate to get them to the area you needed..  It was very awkward.  This unit was also over-engineered with so many fuses that something was always blowing.  So pay attention to the layout....

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, September 13, 2011 10:05 AM
To: histonet@lists.utsouthwestern.edu; StephanieWeaver
Subject: Re: [Histonet] embedding centers

As you correctly point out, all are of similar configuration and foot-print. All use the same technology (a cooling area, a heating bath and a hot?compartment).
As to reliability TissueTek if the best for me. There are some more than 25 years old still?providing a good and reliable service.
Ren? J.

--- On Tue, 9/13/11, Weaver, Stephanie  wrote:


From: Weaver, Stephanie 
Subject: [Histonet] embedding centers
To: "histonet@lists.utsouthwestern.edu" 
Date: Tuesday, September 13, 2011, 9:35 AM


Hello histonetters!

I know this has been asked before, but there's not much in the recent archives.? I'd like to see what everyone thinks is now the best paraffin embedding center.? They all seem very similar now, and I don't see any one instrument that looks much different from the others.? My primary concern is reliability and long working life, but of course I would also like an instrument that is user friendly, ergonomic and affordable.? Please let me know if you have a very good experience with any embedding center or especially if anyone has had a particularly bad experience and let me know any features that you find spectacular or useless.? Thanks for the advice!

Stephanie Weaver
Texas Veterinary Medical Diagnostic Laboratory

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From mpence <@t> grhs.net  Tue Sep 13 09:25:45 2011
From: mpence <@t> grhs.net (Mike Pence)
Date: Tue Sep 13 09:25:53 2011
Subject: [Histonet] P63 CONTROLS
In-Reply-To: 
Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974C9F@is-e2k3.grhs.net>

CC1 standard; antibody 16 minutes @ 37c

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara
Baldwin/mhhcc.org
Sent: Tuesday, September 13, 2011 9:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] P63 CONTROLS


HEY THANKS HISTONETTEERS
For all your help on controls we have the Ventana benchmark LT,  ran the
controls and they are not working maybe we need to tweek our protocol
can anyone help with this ?

Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-996-0210, 0216,  Fax 812-996-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From shive003 <@t> umn.edu  Tue Sep 13 09:59:56 2011
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Tue Sep 13 10:00:04 2011
Subject: [Histonet] Where to start? Neospora and
	ToxoplasmaImmunohistochemistry
References: 
Message-ID: <35E80DEDCBB143A89F6BCD9EC01A9848@auxs.umn.edu>

Hi Leah,

I've done both of these for quite a number of years.  You can write me 
privately with more questions or requests, if you like.

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003@umn.edu

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intended only for the use of the individual or entity to which it is 
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think you have received this message in error, please advise the sender and 
then delete this message and any attachments immediately.)

----- Original Message ----- 
From: 
To: 
Sent: Monday, September 12, 2011 7:43 PM
Subject: [Histonet] Where to start? Neospora and 
ToxoplasmaImmunohistochemistry


> Hi Everyone
>
> I was hoping someone might be able to give me some advice on introducing
> Immunohistochemistry into our Laboratory.
>
> We would like to start initially with Neospora in Cattle and Toxoplasma in
> Sheep.
>
> Is anyone performing these and willing to help me out?
>
> Thank you
>
> Leah Simmons
>
> Industry and Investment NSW
> Elizabeth Macarthur Agricultural Institute
>
> State  Veterinary Diagnostic Laboratory
> Histology
> Technical Officer
> 02 4640 6422
> This message is intended for the addressee named and may contain 
> confidential information. If you are not the intended recipient, please 
> delete it and notify the sender. Views expressed in this message are those 
> of the individual sender, and are not necessarily the views of their 
> organisation.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From nicole <@t> dlcjax.com  Tue Sep 13 10:08:47 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Tue Sep 13 10:12:18 2011
Subject: [Histonet] embedding centers
In-Reply-To: 
References: 
Message-ID: <2436.208.62.167.196.1315926527.squirrel@webmail.realpages.com>

Stephanie,

I prefur the Sakura Tissue-Teks. III being my fav, but it is becomming out
dated and hard to find parts for.  V is good to. The Leica brand is always
a winner. Lots of people have these, so there easy to get parts and have
maintenced. Make sure you check and see how many cassettes the warming
tray can hold. If you processor holds 200, and your embedding cneter only
hold 60 where do you put the rest of the blocks. Make sure the
warming/holding area is large enough to hold all ur blocks.  Good luck. If
you buy refurbished you can save lots of money and with some companies
still get a one year warrenty.

Nicole..



 Hello histonetters!
>
> I know this has been asked before, but there's not much in the recent
> archives.  I'd like to see what everyone thinks is now the best paraffin
> embedding center.  They all seem very similar now, and I don't see any one
> instrument that looks much different from the others.  My primary concern
> is reliability and long working life, but of course I would also like an
> instrument that is user friendly, ergonomic and affordable.  Please let me
> know if you have a very good experience with any embedding center or
> especially if anyone has had a particularly bad experience and let me know
> any features that you find spectacular or useless.  Thanks for the advice!
>
> Stephanie Weaver
> Texas Veterinary Medical Diagnostic Laboratory
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



From nicole <@t> dlcjax.com  Tue Sep 13 10:12:56 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Tue Sep 13 10:16:10 2011
Subject: [Histonet] Facebook
Message-ID: <2473.208.62.167.196.1315926776.squirrel@webmail.realpages.com>

If you havent already come by and liked us, COME CHECK OUT HISTO-VILLE on
facebook.

We have over 100 member so far and hoping to expand. Come by and say hi.

Nicole Tatum, HT ASCP


From pkarlisch <@t> hmc.psu.edu  Tue Sep 13 10:20:41 2011
From: pkarlisch <@t> hmc.psu.edu (Karlisch, Patricia)
Date: Tue Sep 13 10:20:52 2011
Subject: [Histonet] Mismatch Repair positive controls for IHC
Message-ID: 

Hi Histoland,
   We are trying to validate the MMR antibodies MLH1, MSH2 and 6 and PMS2.  We have negative control slides.  Would anyone be willing to send us positive controls for MLH1, MSH 2 and 6 and PMS2.     I know this is a huge request but these are very hard to find controls. If you can help please give me a call or email me directly.
Many thanks in advance.

Pat

Patricia Karlisch
Supervisor, Histology
Pathology and Laboratory Medicine
Penn State Hershey Medical Center
500 University Drive, PA 17033
Tel:  717-531-6072
FAX  717-531-7741
pkarlisch@hmc.psu.edu



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From TJJ <@t> stowers.org  Tue Sep 13 10:35:24 2011
From: TJJ <@t> stowers.org (Johnson, Teri)
Date: Tue Sep 13 10:35:33 2011
Subject: [Histonet] Head,
 Histology and Electron Microscopy - Stowers Institute in
 Kansas City, MO
Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9842035E1@EXCHMB-02.stowers-institute.org>

Dear colleagues,

I am bringing your attention to the following job opening at the Stowers Institute in Kansas City, MO. It is a wonderful place to work, great Principle Investigators and research staff, fantastic physical location and beautiful facility. You can read all about it at www.stowers.org. The following is the ad as listed on the website. If interested, please send resume and coverletter to careers@stowers.org.

Best wishes,
Teri Johnson



Head, Histology and Electron Microscopy

The Stowers Institute for Medical Research has an opening for a Head of Histology and Electron Microscopy to oversee expert delivery of the highest quality service for the detection of gene and protein expression in tissue samples; histochemical staining; sample fixation; routine histology; and ultra-structural analysis.

 Primary responsibilities include maintaining the current status of projects and resolving issues in the EM and Histology labs; actively promoting team interaction and participation; monitoring workload and turn-around time through the LIMS system; providing oversight and feedback on complex or non-routine projects; monitoring usage of services; responding appropriately to unexpected peaks in workload and service requests; maintaining effective communication with all members of the Institute; troubleshooting problems and communicating appropriately with Principal Investigators and the scientific staff, administration, and other core facility personnel as needed; making formal and informal presentations on Core Center services; ensuring continuing education of staff through workshops, webinars, lab meetings, and email communications; and reading professional journals and other sources to stay current in Histologic and EM techniques.

In addition to outstanding communication skills and the ability to effectively multi-task in a team-oriented environment, the successful candidate will have QIHC (ASCP) Qualification, and HT or HTL certification.  Previous management or supervisory experience is preferred.

Minimum requirements include an undergraduate degree in the sciences; excellent knowledge of histologic technique to include fixation, processing, histochemical staining, immunohistochemical staining, microtomy (to include cryostat, paraffin, and plastic), and ultramicrotomy; experience in a research environment with animal model histology; and the ability to apply good judgement to troubleshooting, problem solving, and staff management.  Five to ten years relevant Histology and/or Electron Microscopy experience in lieu of education may be considered.



From pathlocums <@t> gmail.com  Tue Sep 13 11:11:53 2011
From: pathlocums <@t> gmail.com (Davide Costanzo)
Date: Tue Sep 13 11:12:01 2011
Subject: [Histonet] STP420
Message-ID: 

Final hour - still hoping to find users of the Thermo STP420 to provide me
with some feedback. Any comments on this unit??
Thank you.

-- 
*David Costanzo, MHS, PA (ASCP)*
Project Manager
*Blufrog Path Lab Solutions*
9401 Wilshire Blvd. Ste 650
Beverly Hills, CA 90212
From dagill <@t> upei.ca  Tue Sep 13 11:17:59 2011
From: dagill <@t> upei.ca (Daphne Gill)
Date: Tue Sep 13 11:18:08 2011
Subject: [Histonet] TEM using "difficult" tissue
Message-ID: <4E6F58070200000B00007A4E@grpwise.novell.upei.ca>

Hi
I'd like to try some TEM work on rat brain hippocampus. However, the
tissue is less than ideal. Does anyone have any information/insight on
using old (harvested ~2 years ago) tissue that has been subjected to 20
minutes in sodium sulfide (0.4%), fixed using 30% sucrose in 10% BNF for
48 hours, and then frozen? 
Thanks very much for any help you can give me. 
 
Daphne 
From laurie.colbert <@t> huntingtonhospital.com  Tue Sep 13 11:38:53 2011
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Tue Sep 13 11:38:57 2011
Subject: [Histonet] Special Stain / IHC billing - per specimen vs per block
Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2B0DA@EXCHANGE3.huntingtonhospital.com>

Last October, there was a discussion about being able to bill specials
and Immunos (CPT codes 8312, 88313, 88342, 88360, 88361) per block and
not per specimen.  There was a paragraph explaining the change and the
source cited was "CMS /NCCI Updated October 1, 2009."  My manager and I
would like to see the exact source so that we can read the full text.
Does anyone know where we can find the source?

 

Joyce Weems - you may have further info.  Can you help me??

 

Thanks, 

Laurie Colbert                                             

From TJJ <@t> stowers.org  Tue Sep 13 12:16:23 2011
From: TJJ <@t> stowers.org (Johnson, Teri)
Date: Tue Sep 13 12:16:27 2011
Subject: [Histonet] Re: TEM using "difficult" tissue
Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9842035EE@EXCHMB-02.stowers-institute.org>

Daphne,

While I can appreciate wanting to try something just to see if it might be helpful, I really fail to see any benefit from trying to look at ultrastructure of tissues that have been fixed and stored in this way. The ultrastructure will be compromised, and it will be difficult to get any useful information from fixation or storage artifact in the tissues. You would need to validate your findings on freshly, properly fixed materials anyway, so why not just start with the best possible chance for success and use the right stuff right off the bat?

Good luck no matter which way you decide to proceed.

Teri Johnson
Stowers Institute for Medical Research
Kansas City, MO

From MSHERWOOD <@t> PARTNERS.ORG  Tue Sep 13 13:05:42 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret)
Date: Tue Sep 13 13:05:50 2011
Subject: [Histonet] Facebook
In-Reply-To: <2473.208.62.167.196.1315926776.squirrel@webmail.realpages.com>
References: <2473.208.62.167.196.1315926776.squirrel@webmail.realpages.com>
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB59C6@PHSXMB30.partners.org>

How is this site listed?  I searched for Histo-Ville, but nothing came up. 


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum
Sent: Tuesday, September 13, 2011 11:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Facebook

If you havent already come by and liked us, COME CHECK OUT HISTO-VILLE on
facebook.

We have over 100 member so far and hoping to expand. Come by and say hi.

Nicole Tatum, HT ASCP


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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From mcauliff <@t> umdnj.edu  Tue Sep 13 13:24:52 2011
From: mcauliff <@t> umdnj.edu (Geoff McAuliffe)
Date: Tue Sep 13 13:24:13 2011
Subject: [Histonet] TEM using "difficult" tissue
In-Reply-To: <4E6F58070200000B00007A4E@grpwise.novell.upei.ca>
References: <4E6F58070200000B00007A4E@grpwise.novell.upei.ca>
Message-ID: <4E6F9FF4.5000201@umdnj.edu>

I suspect it will be somewhere between rather ugly to totally worthless, 
more likely the latter.
The only was to know is to find out for yourself. Thaw, rinse multiple 
times in buffer, osmicate in buffer, rinse, dehydrate, clear and embed 
in your favorite epoxy.
Good luck.

Geoff

On 9/13/2011 12:17 PM, Daphne Gill wrote:
> Hi
> I'd like to try some TEM work on rat brain hippocampus. However, the
> tissue is less than ideal. Does anyone have any information/insight on
> using old (harvested ~2 years ago) tissue that has been subjected to 20
> minutes in sodium sulfide (0.4%), fixed using 30% sucrose in 10% BNF for
> 48 hours, and then frozen?
> Thanks very much for any help you can give me.
>
> Daphne
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff@umdnj.edu
**********************************************



From schaundrawalton <@t> yahoo.com  Tue Sep 13 13:35:16 2011
From: schaundrawalton <@t> yahoo.com (Schaundra Walton)
Date: Tue Sep 13 13:35:19 2011
Subject: [Histonet] Dako H&E Stainer
Message-ID: <1315938916.8864.YahooMailNeo@web120604.mail.ne1.yahoo.com>

Hey there Histonetters!
?
Does anybody have any feedback regarding the Dako CoverStainer?? This is an H&E stainer with a glass coverslipper attached.? We are looking at one and I would be interested in getting feedback from someone who is using or has used it.? How does it compare to the TissueTek Prisma or Shandon Gemini?? Ventana?? You can PM responses to me if you like.? 
?
Thanks in advance for the help!

Schaundra Walton BS HTL(ASCP)
From madary <@t> verizon.net  Tue Sep 13 14:12:58 2011
From: madary <@t> verizon.net (madary@verizon.net)
Date: Tue Sep 13 14:13:12 2011
Subject: [Histonet] embedding centers
Message-ID: <6729370.4042490.1315941178242.JavaMail.root@vms170033>



    I  would  stay  away from Medite. THey are poorly designed and do not
   talk to each other. Temps are bad and service even under warranty does
   not  exist.   The embedding mold bin was at the highest temp and still
   could  not  mel  the paraffin.Tried to chat with a rep at a conference
   whohad  sold  me  the  unit to tell her what happened and she left the
   conference  early  rather  than  deal with the issue. Don't waste your
   time  and  money.  Lots  of  great  companies  out there with superior
   embedding centers.
   Nick(Rocky) Madary, HT/HTL(ASCP)QIHC
From jcox90 <@t> yahoo.com  Tue Sep 13 14:28:13 2011
From: jcox90 <@t> yahoo.com (Jill Cox)
Date: Tue Sep 13 14:27:25 2011
Subject: [Histonet] embedding centers
In-Reply-To: <6729370.4042490.1315941178242.JavaMail.root@vms170033>
References: <6729370.4042490.1315941178242.JavaMail.root@vms170033>
Message-ID: <9B836883-FA28-4489-8589-F08FF6753ADA@yahoo.com>

I agree! We just sent our brand new Medite back to vendor because of problems. The cold spot was icing over and dispenser was dispensing paraffin when I wasn't even using it!  We just purchased the Sakura tec which has been my favorite since I've been a tech. 

Sent from my iPhone

On Sep 13, 2011, at 12:12 PM, madary@verizon.net wrote:

> 
> 
>    I  would  stay  away from Medite. THey are poorly designed and do not
>   talk to each other. Temps are bad and service even under warranty does
>   not  exist.   The embedding mold bin was at the highest temp and still
>   could  not  mel  the paraffin.Tried to chat with a rep at a conference
>   whohad  sold  me  the  unit to tell her what happened and she left the
>   conference  early  rather  than  deal with the issue. Don't waste your
>   time  and  money.  Lots  of  great  companies  out there with superior
>   embedding centers.
>   Nick(Rocky) Madary, HT/HTL(ASCP)QIHC
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From shive003 <@t> umn.edu  Tue Sep 13 14:59:05 2011
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Tue Sep 13 14:59:09 2011
Subject: [Histonet] TEM using "difficult" tissue
References: <4E6F58070200000B00007A4E@grpwise.novell.upei.ca>
Message-ID: <9BD1F6C9CF5A4977ABF8417321E80DA6@auxs.umn.edu>

Daphne,

We do EM work on formalin-fixed, paraffin-embedded tissue occasionally.  I 
will send you a brief protocol under separate cover.

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003@umn.edu

(Confidentiality Notice: This message, together with any attachments, is 
intended only for the use of the individual or entity to which it is 
addressed and may contain confidential or privileged information. If you 
think you have received this message in error, please advise the sender and 
then delete this message and any attachments immediately.)

----- Original Message ----- 
From: "Daphne Gill" 
To: 
Sent: Tuesday, September 13, 2011 11:17 AM
Subject: [Histonet] TEM using "difficult" tissue


> Hi
> I'd like to try some TEM work on rat brain hippocampus. However, the
> tissue is less than ideal. Does anyone have any information/insight on
> using old (harvested ~2 years ago) tissue that has been subjected to 20
> minutes in sodium sulfide (0.4%), fixed using 30% sucrose in 10% BNF for
> 48 hours, and then frozen?
> Thanks very much for any help you can give me.
>
> Daphne
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From thiggins <@t> cddmedical.com  Tue Sep 13 15:06:47 2011
From: thiggins <@t> cddmedical.com (Tim Higgins)
Date: Tue Sep 13 15:06:50 2011
Subject: [Histonet] STP420ES
References: <20110913170345.65749127AC54@barracuda.crvinc.net>
Message-ID: <002101cc7250$ab0b0cc0$e001a8c0@cdd.loc>

Good instument, been working with it for about 6 months, using the Fast Flex
system for rapid tissue processing.  What did you want to know specifically?

Tim

Message: 8
Date: Tue, 13 Sep 2011 09:11:53 -0700
From: Davide Costanzo 
Subject: [Histonet] STP420
To: histonet@lists.utsouthwestern.edu
Message-ID:

Content-Type: text/plain; charset=ISO-8859-1

Final hour - still hoping to find users of the Thermo STP420 to provide me
with some feedback. Any comments on this unit??
Thank you.

-- 
*David Costanzo, MHS, PA (ASCP)*
Project Manager
*Blufrog Path Lab Solutions*
9401 Wilshire Blvd. Ste 650
Beverly Hills, CA 90212


From Carol.Fields <@t> Northside.com  Tue Sep 13 15:18:45 2011
From: Carol.Fields <@t> Northside.com (Carol Fields)
Date: Tue Sep 13 15:18:55 2011
Subject: FW: [Histonet] embedding centers
Message-ID: <731941C266951A47BEF11E5EFAAED9C90B666C4E@nsmvexch01.northside.local>



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
madary@verizon.net
Sent: Tuesday, September 13, 2011 3:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding centers



    I  would  stay  away from Medite. THey are poorly designed and do
not
   talk to each other. Temps are bad and service even under warranty
does
   not  exist.   The embedding mold bin was at the highest temp and
still
   could  not  mel  the paraffin.Tried to chat with a rep at a
conference
   whohad  sold  me  the  unit to tell her what happened and she left
the
   conference  early  rather  than  deal with the issue. Don't waste
your
   time  and  money.  Lots  of  great  companies  out there with
superior
   embedding centers.
   Nick(Rocky) Madary, HT/HTL(ASCP)QIHC
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege.


From nicole <@t> dlcjax.com  Tue Sep 13 15:15:52 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Tue Sep 13 15:19:07 2011
Subject: [Histonet] Facebook
In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB59C6@PHSXMB30.partners.org>
References: <2473.208.62.167.196.1315926776.squirrel@webmail.realpages.com>
	<073AE2BEA1C2BA4A8837AB6C4B943D9708DB59C6@PHSXMB30.partners.org>
Message-ID: <3306.208.62.167.196.1315944952.squirrel@webmail.realpages.com>

Under medical community




How is this site listed?  I searched for Histo-Ville, but nothing came up.
>
>
> Peggy Sherwood
> Lab Associate, Photopathology
> Wellman Center for Photomedicine (EDR 214)
> Massachusetts General Hospital
> 50 Blossom Street
> Boston, MA 02114-2696
> 617-724-4839 (voice mail)
> 617-726-6983 (lab)
> 617-726-1206 (fax)
> msherwood@partners.org
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole
> Tatum
> Sent: Tuesday, September 13, 2011 11:13 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Facebook
>
> If you havent already come by and liked us, COME CHECK OUT HISTO-VILLE on
> facebook.
>
> We have over 100 member so far and hoping to expand. Come by and say hi.
>
> Nicole Tatum, HT ASCP
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> The information in this e-mail is intended only for the person to whom it
> is
> addressed. If you believe this e-mail was sent to you in error and the
> e-mail
> contains patient information, please contact the Partners Compliance
> HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in
> error
> but does not contain patient information, please contact the sender and
> properly
> dispose of the e-mail.
>
>



From sdysart <@t> mirnarx.com  Tue Sep 13 15:22:32 2011
From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com)
Date: Tue Sep 13 15:22:36 2011
Subject: [Histonet] Facebook
In-Reply-To: <3306.208.62.167.196.1315944952.squirrel@webmail.realpages.com>
References: <2473.208.62.167.196.1315926776.squirrel@webmail.realpages.com><073AE2BEA1C2BA4A8837AB6C4B943D9708DB59C6@PHSXMB30.partners.org>
	<3306.208.62.167.196.1315944952.squirrel@webmail.realpages.com>
Message-ID: 

You can search it in groups too...it has and H&E heart....well actually
it's some kind of lumen in the shape of a heart.  I don't remember the
specific organ =)

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole
Tatum
Sent: Tuesday, September 13, 2011 3:16 PM
To: Sherwood, Margaret; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Facebook

Under medical community




How is this site listed?  I searched for Histo-Ville, but nothing came
up.
>
>
> Peggy Sherwood
> Lab Associate, Photopathology
> Wellman Center for Photomedicine (EDR 214)
> Massachusetts General Hospital
> 50 Blossom Street
> Boston, MA 02114-2696
> 617-724-4839 (voice mail)
> 617-726-6983 (lab)
> 617-726-1206 (fax)
> msherwood@partners.org
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole
> Tatum
> Sent: Tuesday, September 13, 2011 11:13 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Facebook
>
> If you havent already come by and liked us, COME CHECK OUT HISTO-VILLE
on
> facebook.
>
> We have over 100 member so far and hoping to expand. Come by and say
hi.
>
> Nicole Tatum, HT ASCP
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> The information in this e-mail is intended only for the person to whom
it
> is
> addressed. If you believe this e-mail was sent to you in error and the
> e-mail
> contains patient information, please contact the Partners Compliance
> HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you
in
> error
> but does not contain patient information, please contact the sender
and
> properly
> dispose of the e-mail.
>
>



_______________________________________________
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From Carol.Fields <@t> Northside.com  Tue Sep 13 15:33:07 2011
From: Carol.Fields <@t> Northside.com (Carol Fields)
Date: Tue Sep 13 15:33:15 2011
Subject: FW: [Histonet] embedding centers
Message-ID: <731941C266951A47BEF11E5EFAAED9C90B666C4F@nsmvexch01.northside.local>


-----Original Message-----
From: Carol Fields 
Sent: Tuesday, September 13, 2011 4:18 PM
To: 'madary@verizon.net'
Cc: 'mailto:histonet-bounces@lists.utsouthwestern.edu'
Subject: RE: [Histonet] embedding centers



I bought 3 microtomes from Medite and they would not take them back
(they would swap them out) and would not refund my money..... plus I
received a really nasty note from one of the head people of Medite for
even asking about the company on the Histo Net. I told him the Histo Net
was for techs to say what they wanted not vendors.  Watch out.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
madary@verizon.net
Sent: Tuesday, September 13, 2011 3:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding centers



    I  would  stay  away from Medite. THey are poorly designed and do
not
   talk to each other. Temps are bad and service even under warranty
does
   not  exist.   The embedding mold bin was at the highest temp and
still
   could  not  mel  the paraffin.Tried to chat with a rep at a
conference
   whohad  sold  me  the  unit to tell her what happened and she left
the
   conference  early  rather  than  deal with the issue. Don't waste
your
   time  and  money.  Lots  of  great  companies  out there with
superior
   embedding centers.
   Nick(Rocky) Madary, HT/HTL(ASCP)QIHC
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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From amosbrooks <@t> gmail.com  Tue Sep 13 15:33:48 2011
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Tue Sep 13 15:33:53 2011
Subject: [Histonet] immunohistochemistry: manual staining
Message-ID: 

Hi,
     Shandon Sequenza staining racks and slide clips. They are great. You
don't need the whole system (timers & boxes to hold the reagents). Basically
this is a rack that holds slides and slide clips vertically and the reagents
are dropped in at the top of the slide and it displaces the reagents below.
It is great for manual IHC. Drop me an email if you want some more info.

Amos

On Tue, Sep 13, 2011 at 10:06 AM,  wrote:

> Message: 12
> Date: Tue, 13 Sep 2011 14:08:53 +0200
> From: "Prof. dr. Wim Van den Broeck" 
> Subject: [Histonet] immunohistochemistry: manual staining
> To: 
> Message-ID: <004401cc720d$e801b8b0$b8052a10$@vandenbroeck@UGent.be>
> Content-Type: text/plain;       charset="us-ascii"
>
> Dear Colleagues,
>
> We do a lot of immunohistochemical stainings (research, no diagnostics),
> but
> unfortunately, we cannot afford an automated stainer. At the moment, we do
> everything manually (washing, incubating, ....) but I would like to make
> things run more smoothly. So my question is, is there any device or systems
> that can be helpful during manual IHC stainings ?
>
> Thanks in advance for your comments / suggestions.
>
> --
> Met vriendelijke groeten,
>
From sdysart <@t> mirnarx.com  Tue Sep 13 15:36:25 2011
From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com)
Date: Tue Sep 13 15:36:29 2011
Subject: [Histonet] immunohistochemistry: manual staining
In-Reply-To: 
References: 
Message-ID: 

I tried ordering this system a few months ago, and it was discontinued?

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos
Brooks
Sent: Tuesday, September 13, 2011 3:34 PM
To: histonet@lists.utsouthwestern.edu; wim.vandenbroeck@UGent.be
Subject: [Histonet] immunohistochemistry: manual staining

Hi,
     Shandon Sequenza staining racks and slide clips. They are great.
You
don't need the whole system (timers & boxes to hold the reagents).
Basically
this is a rack that holds slides and slide clips vertically and the
reagents
are dropped in at the top of the slide and it displaces the reagents
below.
It is great for manual IHC. Drop me an email if you want some more info.

Amos

On Tue, Sep 13, 2011 at 10:06 AM,
 wrote:

> Message: 12
> Date: Tue, 13 Sep 2011 14:08:53 +0200
> From: "Prof. dr. Wim Van den Broeck" 
> Subject: [Histonet] immunohistochemistry: manual staining
> To: 
> Message-ID: <004401cc720d$e801b8b0$b8052a10$@vandenbroeck@UGent.be>
> Content-Type: text/plain;       charset="us-ascii"
>
> Dear Colleagues,
>
> We do a lot of immunohistochemical stainings (research, no
diagnostics),
> but
> unfortunately, we cannot afford an automated stainer. At the moment,
we do
> everything manually (washing, incubating, ....) but I would like to
make
> things run more smoothly. So my question is, is there any device or
systems
> that can be helpful during manual IHC stainings ?
>
> Thanks in advance for your comments / suggestions.
>
> --
> Met vriendelijke groeten,
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From BDeBrosse-Serra <@t> isisph.com  Tue Sep 13 15:42:52 2011
From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra)
Date: Tue Sep 13 15:43:06 2011
Subject: [Histonet] immunohistochemistry: manual staining
In-Reply-To: 
References: 
	
Message-ID: <493CAA64F203E14E8823737B9EE0E25F091D4EDE31@EXCHMB01.isis.local>

It is discontinued. I went on line, and that is what it said. 

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
1896 Rutherford Road
Carlsbad, CA 92008
760-603-2371



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sdysart@mirnarx.com
Sent: Tuesday, September 13, 2011 1:36 PM
To: amosbrooks@gmail.com; histonet@lists.utsouthwestern.edu; wim.vandenbroeck@UGent.be
Subject: RE: [Histonet] immunohistochemistry: manual staining

I tried ordering this system a few months ago, and it was discontinued?

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos
Brooks
Sent: Tuesday, September 13, 2011 3:34 PM
To: histonet@lists.utsouthwestern.edu; wim.vandenbroeck@UGent.be
Subject: [Histonet] immunohistochemistry: manual staining

Hi,
     Shandon Sequenza staining racks and slide clips. They are great.
You
don't need the whole system (timers & boxes to hold the reagents).
Basically
this is a rack that holds slides and slide clips vertically and the
reagents
are dropped in at the top of the slide and it displaces the reagents
below.
It is great for manual IHC. Drop me an email if you want some more info.

Amos

On Tue, Sep 13, 2011 at 10:06 AM,
 wrote:

> Message: 12
> Date: Tue, 13 Sep 2011 14:08:53 +0200
> From: "Prof. dr. Wim Van den Broeck" 
> Subject: [Histonet] immunohistochemistry: manual staining
> To: 
> Message-ID: <004401cc720d$e801b8b0$b8052a10$@vandenbroeck@UGent.be>
> Content-Type: text/plain;       charset="us-ascii"
>
> Dear Colleagues,
>
> We do a lot of immunohistochemical stainings (research, no
diagnostics),
> but
> unfortunately, we cannot afford an automated stainer. At the moment,
we do
> everything manually (washing, incubating, ....) but I would like to
make
> things run more smoothly. So my question is, is there any device or
systems
> that can be helpful during manual IHC stainings ?
>
> Thanks in advance for your comments / suggestions.
>
> --
> Met vriendelijke groeten,
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From amosbrooks <@t> gmail.com  Tue Sep 13 16:27:33 2011
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Tue Sep 13 16:27:38 2011
Subject: [Histonet] immunohistochemistry: manual staining
In-Reply-To: <493CAA64F203E14E8823737B9EE0E25F091D4EDE31@EXCHMB01.isis.local>
References: 
	
	<493CAA64F203E14E8823737B9EE0E25F091D4EDE31@EXCHMB01.isis.local>
Message-ID: 

WOW is my bottom lip ever puffy! (Sad face) That thing was awesome. Does
anyone know of anything similar? I had been wanting to get an extra rack
just in case. That reminds me of my favorite Mae West quote "He who
hesitates is a d@mned fool."

Amos

On Tue, Sep 13, 2011 at 4:42 PM, Bea DeBrosse-Serra <
BDeBrosse-Serra@isisph.com> wrote:

> It is discontinued. I went on line, and that is what it said.
>
> Beatrice DeBrosse-Serra HT(ASCP)QIHC
> Isis Pharmaceuticals
> Antisense Drug Discovery
> 1896 Rutherford Road
> Carlsbad, CA 92008
> 760-603-2371
>
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:
> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> sdysart@mirnarx.com
> Sent: Tuesday, September 13, 2011 1:36 PM
> To: amosbrooks@gmail.com; histonet@lists.utsouthwestern.edu;
> wim.vandenbroeck@UGent.be
> Subject: RE: [Histonet] immunohistochemistry: manual staining
>
> I tried ordering this system a few months ago, and it was discontinued?
>
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas  78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos
> Brooks
> Sent: Tuesday, September 13, 2011 3:34 PM
> To: histonet@lists.utsouthwestern.edu; wim.vandenbroeck@UGent.be
> Subject: [Histonet] immunohistochemistry: manual staining
>
> Hi,
>     Shandon Sequenza staining racks and slide clips. They are great.
> You
> don't need the whole system (timers & boxes to hold the reagents).
> Basically
> this is a rack that holds slides and slide clips vertically and the
> reagents
> are dropped in at the top of the slide and it displaces the reagents
> below.
> It is great for manual IHC. Drop me an email if you want some more info.
>
> Amos
>
> On Tue, Sep 13, 2011 at 10:06 AM,
>  > wrote:
>
> > Message: 12
> > Date: Tue, 13 Sep 2011 14:08:53 +0200
> > From: "Prof. dr. Wim Van den Broeck" 
> > Subject: [Histonet] immunohistochemistry: manual staining
> > To: 
> > Message-ID: <004401cc720d$e801b8b0$b8052a10$@vandenbroeck@UGent.be>
> > Content-Type: text/plain;       charset="us-ascii"
> >
> > Dear Colleagues,
> >
> > We do a lot of immunohistochemical stainings (research, no
> diagnostics),
> > but
> > unfortunately, we cannot afford an automated stainer. At the moment,
> we do
> > everything manually (washing, incubating, ....) but I would like to
> make
> > things run more smoothly. So my question is, is there any device or
> systems
> > that can be helpful during manual IHC stainings ?
> >
> > Thanks in advance for your comments / suggestions.
> >
> > --
> > Met vriendelijke groeten,
> >
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From Grant.Madill <@t> deedi.qld.gov.au  Tue Sep 13 21:04:25 2011
From: Grant.Madill <@t> deedi.qld.gov.au (Madill, Grant)
Date: Tue Sep 13 21:04:33 2011
Subject: [Histonet] Spirochete Sensitizer
Message-ID: <4A91E88A30D63B4EAA9E25EF05D984E60395104F@pibexhpr04.dpi.qld.gov.au>

Hi Randi
I use 0.5% Iodine in 70% Alcohol as a pretreatment to the Warthin-Starry
method to enhance Leptospira, Brachyspira and Lawsonia sp.
I find it works very well.

After bringing sections to deionised water treat with 0.5% Iodine in 70%
Alcohol (prepare fresh) for 5 min. 
Treat in 5% Sodium Thiosulphate. The Iodine - brown (I2) immediately
becomes Iodide - colourless (I).
Wash in running tap water for 5 min. Rinse well in deionised water then
proceed with Warthin-Starry. 

I've found over the years that good silver staining depends on proper
fixation.

Hope this helps.
Grant Madill
Laboratory Technician 
Animal Disease Surveillance Laboratory
Biosecurity Queensland 
Department of Employment, Economic Development and Innovation
203 Tor Street, Toowoomba Qld 4350

We're behind the Bid!
GOLD COAST 2018 - XXI COMMONWEALTH GAMES CANDIDATE CITY
www.goldcoast2018bid.com

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this communication in error, please notify the sender immediately 
and delete it from your computer system network.
From jqb7 <@t> cdc.gov  Wed Sep 14 04:58:14 2011
From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID))
Date: Wed Sep 14 04:58:20 2011
Subject: [Histonet] Dako H&E Stainer
In-Reply-To: <1315938916.8864.YahooMailNeo@web120604.mail.ne1.yahoo.com>
References: <1315938916.8864.YahooMailNeo@web120604.mail.ne1.yahoo.com>
Message-ID: 

I have not used it but have heard some good things.  I was told that it is large (floor model?) and is best for labs with really large volumes

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton
Sent: Tuesday, September 13, 2011 2:35 PM
To: Histonet
Subject: [Histonet] Dako H&E Stainer

Hey there Histonetters!
?
Does anybody have any feedback regarding the Dako CoverStainer?? This is an H&E stainer with a glass coverslipper attached.? We are looking at one and I would be interested in getting feedback from someone who is using or has used it.? How does it compare to the TissueTek Prisma or Shandon Gemini?? Ventana?? You can PM responses to me if you like.? 
?
Thanks in advance for the help!

Schaundra Walton BS HTL(ASCP)

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From tgenade <@t> gmail.com  Wed Sep 14 06:52:08 2011
From: tgenade <@t> gmail.com (Tyrone Genade)
Date: Wed Sep 14 06:52:16 2011
Subject: [Histonet] Bouin's without the picric acid?
Message-ID: 

Hello,

I have been using Bouin's fixative on my fish specimens. I had simply
been killing the fish, slitting them down their belly and letting them
lie in Bouin's overnight. This worked well to soften the tissue and in
particular the brain case (I'm interested in the brain but the
dissection is delicate and I'm clumsy). After trying some silver
staining the results were not very good. I have since learned that
picric acid interferes with silver staining (and fluorescent staining
like FluoroJade-B) so no more picric acid for me.

My question is this: can I use Buoin's without picric acid? The
solution is 75 mL saturated picric acid, 25 mL 40% formaldehyde and 5
mL glacial acetic acid to make 100 mL.  If I simply left out the
picric acid, replacing it with distilled water, would this cause
trouble? I am reluctant to switch to PFA as this can destroy certain
antibody epitopes whereas the Bouin's doesn't (so I'm told but I was
also told that Buoin's won't cause trouble at all...).

My other idea was to use PFA and then decalcify in dilute acetic or formic acid.

Any suggestions or advice?

Thanks
-- 
Tyrone Genade
http://tgenade.freeshell.org
email: tgenade@gmail.com
tel: +27-84-632-1925 (c)
********************************************************************************
Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord.
To find out how to receive this FREE gift visit http://www.alpha.org.

From laurie.colbert <@t> huntingtonhospital.com  Wed Sep 14 09:20:42 2011
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Wed Sep 14 09:20:51 2011
Subject: [Histonet] Bouin's without the picric acid?
In-Reply-To: 
References: 
Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2B0E0@EXCHANGE3.huntingtonhospital.com>

Tyrone,

American Master Tech carries a product called Bouins 2000 which contains
no picric acid.  Search it on their website - they carry it in various
sizes.'

Laurie Colbert

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tyrone
Genade
Sent: Wednesday, September 14, 2011 4:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bouin's without the picric acid?

Hello,

I have been using Bouin's fixative on my fish specimens. I had simply
been killing the fish, slitting them down their belly and letting them
lie in Bouin's overnight. This worked well to soften the tissue and in
particular the brain case (I'm interested in the brain but the
dissection is delicate and I'm clumsy). After trying some silver
staining the results were not very good. I have since learned that
picric acid interferes with silver staining (and fluorescent staining
like FluoroJade-B) so no more picric acid for me.

My question is this: can I use Buoin's without picric acid? The
solution is 75 mL saturated picric acid, 25 mL 40% formaldehyde and 5
mL glacial acetic acid to make 100 mL.  If I simply left out the
picric acid, replacing it with distilled water, would this cause
trouble? I am reluctant to switch to PFA as this can destroy certain
antibody epitopes whereas the Bouin's doesn't (so I'm told but I was
also told that Buoin's won't cause trouble at all...).

My other idea was to use PFA and then decalcify in dilute acetic or
formic acid.

Any suggestions or advice?

Thanks
-- 
Tyrone Genade
http://tgenade.freeshell.org
email: tgenade@gmail.com
tel: +27-84-632-1925 (c)
************************************************************************
********
Romans 6:23: The gift of God is eternal life through Christ Jesus our
Lord.
To find out how to receive this FREE gift visit http://www.alpha.org.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From mcauliff <@t> umdnj.edu  Wed Sep 14 09:25:26 2011
From: mcauliff <@t> umdnj.edu (Geoff McAuliffe)
Date: Wed Sep 14 09:24:49 2011
Subject: [Histonet] Bouin's without the picric acid?
In-Reply-To: 
References: 
Message-ID: <4E70B956.8070707@umdnj.edu>

Hi Tyrone:

Picric acid may be bad for some silver stains but not for all. Williams 
fix is recommended for the Bodain method and I have used it with 
excellent results on mouse brain.
Also overnight immersion fixation of a whole fish - you did not say how 
large- is not going to give good results. The 3 components of Bouin all 
penetrate and fix at different rates.
I would fix for a week, room temp. with the head cut off and maybe the 
mandible removed to allow better penetration.
Bouin without picric acid is just an alcoholic formalin but if you want 
to avoid formalin (PFA) then how about an acid alchohol fix, 70 or 80 
percent EtOH with 5% acetic acid?

Geoff

On 9/14/2011 7:52 AM, Tyrone Genade wrote:
> Hello,
>
> I have been using Bouin's fixative on my fish specimens. I had simply
> been killing the fish, slitting them down their belly and letting them
> lie in Bouin's overnight. This worked well to soften the tissue and in
> particular the brain case (I'm interested in the brain but the
> dissection is delicate and I'm clumsy). After trying some silver
> staining the results were not very good. I have since learned that
> picric acid interferes with silver staining (and fluorescent staining
> like FluoroJade-B) so no more picric acid for me.
>
> My question is this: can I use Buoin's without picric acid? The
> solution is 75 mL saturated picric acid, 25 mL 40% formaldehyde and 5
> mL glacial acetic acid to make 100 mL.  If I simply left out the
> picric acid, replacing it with distilled water, would this cause
> trouble? I am reluctant to switch to PFA as this can destroy certain
> antibody epitopes whereas the Bouin's doesn't (so I'm told but I was
> also told that Buoin's won't cause trouble at all...).
>
> My other idea was to use PFA and then decalcify in dilute acetic or formic acid.
>
> Any suggestions or advice?
>
> Thanks


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff@umdnj.edu
**********************************************



From rjbuesa <@t> yahoo.com  Wed Sep 14 09:49:02 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Sep 14 09:49:11 2011
Subject: [Histonet] Bouin's without the picric acid?
In-Reply-To: 
Message-ID: <1316011742.56614.YahooMailClassic@web65715.mail.ac4.yahoo.com>

Bouin's without picric acid is an oxymoron, is like saying "neutral buffered formalin without the buffer".
By definition, and resulting from the?original formula, Bouin requires picric acid, that is the whole rationale behind this fixative.
There are "commercial" products called "Bouin" that do not contain picric acid, and they are just not Bouin, they may be anything else, but not Bouin. Why? Because a commercial manufacturer can call anything as long as they can sell it, but that is no guarantee to be true.
In your case if you use "Bouin without picric acid" you may improve the silver staining, but you will no longer be able to obtain the softening of the tissue, which is a characteristic reaction to the picric acid. So make your pick which result you prefer but, please, do not call Bouin to a fixative without picric acid!
Ren? J.

--- On Wed, 9/14/11, Tyrone Genade  wrote:


From: Tyrone Genade 
Subject: [Histonet] Bouin's without the picric acid?
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, September 14, 2011, 7:52 AM


Hello,

I have been using Bouin's fixative on my fish specimens. I had simply
been killing the fish, slitting them down their belly and letting them
lie in Bouin's overnight. This worked well to soften the tissue and in
particular the brain case (I'm interested in the brain but the
dissection is delicate and I'm clumsy). After trying some silver
staining the results were not very good. I have since learned that
picric acid interferes with silver staining (and fluorescent staining
like FluoroJade-B) so no more picric acid for me.

My question is this: can I use Buoin's without picric acid? The
solution is 75 mL saturated picric acid, 25 mL 40% formaldehyde and 5
mL glacial acetic acid to make 100 mL.? If I simply left out the
picric acid, replacing it with distilled water, would this cause
trouble? I am reluctant to switch to PFA as this can destroy certain
antibody epitopes whereas the Bouin's doesn't (so I'm told but I was
also told that Buoin's won't cause trouble at all...).

My other idea was to use PFA and then decalcify in dilute acetic or formic acid.

Any suggestions or advice?

Thanks
-- 
Tyrone Genade
http://tgenade.freeshell.org
email: tgenade@gmail.com
tel: +27-84-632-1925 (c)
********************************************************************************
Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord.
To find out how to receive this FREE gift visit http://www.alpha.org.

_______________________________________________
Histonet mailing list
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From nicole <@t> dlcjax.com  Wed Sep 14 09:47:43 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Wed Sep 14 09:51:15 2011
Subject: [Histonet] Pathlogix
Message-ID: <1098.208.62.167.196.1316011663.squirrel@webmail.realpages.com>

To those of you utilizing this software. Or if I have already spoken with
you about it. Please contact me throught me personal email. I have figured
it out!!!!!!!

Nicole Tatum
Nicole@dlcjax.com


From rsrichmond <@t> gmail.com  Wed Sep 14 10:05:03 2011
From: rsrichmond <@t> gmail.com (Bob Richmond)
Date: Wed Sep 14 10:05:11 2011
Subject: [Histonet] Re: Bouin's without the picric acid?
Message-ID: 

Bouin's without the picric acid?

"Bouin's fixative" without picric acid isn't Bouin's fixative, any
more than a "chocolate cake" without the chocolate is a chocolate
cake.

"Whatever our marketing department calls Bouin's fixative" is junk
science. We see the same thing with bogus B-5 fixatives, and iodate
hematoxylins labeled "Harris".

You need some expert help with your need to change fixatives. I'd
suggest asking John Kiernan - I'm pretty sure he's still on Histonet.

Bob Richmond
Samurai Pathologist
Knoxville TN

From ccrowder <@t> vetmed.lsu.edu  Wed Sep 14 12:14:27 2011
From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder)
Date: Wed Sep 14 12:15:40 2011
Subject: [Histonet] Bouin's without picric acid
Message-ID: 

Tyrone - 
I have been working with fish (whole and pieces and parts) for years.  The 
fixative of choice  for whole fish as been Davidson's.  It contains 
formalin, alcohol, acetic acid and water.  After the whole fish is opened,  
the acetic acid begins to soften the harder tissues  while the formalin 
fixes the  rest.  Since there is alcohol, the tissues are then placed in 70% 
alcohol and processed.   The length of time for fixation depends on the size 
of the fish of course.  You'll just have to find the times.  Many of the 
small fish (fingerlings) have been  ready in 24 hours.  But many of the fish 
we get have been fixed for a week or more and results are really good.  
Contact me personally if you need more information.
Cheryl


Cheryl Crowder, BA, HTL(ASCP)
Crowder Histology Consulting
4952 Alvin Dark Ave.
Baton Rouge, LA  70820
(225)  772-2865
From CThornton <@t> dahlchase.com  Wed Sep 14 15:07:46 2011
From: CThornton <@t> dahlchase.com (Clare Thornton)
Date: Wed Sep 14 15:07:50 2011
Subject: [Histonet] CAP GEN.41770 Glassware cleaning
Message-ID: 

This checklist question has a sample procedure for how to check for residual detergent in glassware.  The procedure says to use a pH meter.  We've always used pH paper.  However, in reading their recommended procedure, you need to look for a change of greater than 0.2 pH units between source water and glassware pH.  Does anyone else use pH paper vs. a pH meter?  We only use our pH meter very infrequently and will calibrate it just prior to use.  If we use it for glassware cleaning, we'll have to calibrate more regularly, something I'd just as soon avoid if possible.  We've never had a problem with our glassware cleaning procedure with CAP in the past.  Any advice?

Clare J. Thornton, HTL(ASCP), QIHC
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthornton@dahlchase.com

From rjbuesa <@t> yahoo.com  Wed Sep 14 15:59:07 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Sep 14 15:59:11 2011
Subject: [Histonet] CAP GEN.41770 Glassware cleaning
In-Reply-To: 
Message-ID: <1316033947.95063.YahooMailClassic@web65702.mail.ac4.yahoo.com>

Using?a pH meter requires that you add dist. water to the glass flask, swirl it around and "hope" that something will be "trapped" by that water that will give you an idea of the residual pH that may be in the glass.
Both things are quite improbable.
Instead of the pH meter or the paper indicator I always added to the water from the flask 
few drops of bromothymol blue that would turn yellow at pH<7 and blue at pH>7
On the other hand if the glass is properly washed in distilled water you will never get a pH other than neutral.
Ren? J.
?
?
?

--- On Wed, 9/14/11, Clare Thornton  wrote:


From: Clare Thornton 
Subject: [Histonet] CAP GEN.41770 Glassware cleaning
To: "'Histonet@lists.utsouthwestern.edu'" 
Date: Wednesday, September 14, 2011, 4:07 PM


This checklist question has a sample procedure for how to check for residual detergent in glassware.? The procedure says to use a pH meter.? We've always used pH paper.? However, in reading their recommended procedure, you need to look for a change of greater than 0.2 pH units between source water and glassware pH.? Does anyone else use pH paper vs. a pH meter?? We only use our pH meter very infrequently and will calibrate it just prior to use.? If we use it for glassware cleaning, we'll have to calibrate more regularly, something I'd just as soon avoid if possible.? We've never had a problem with our glassware cleaning procedure with CAP in the past.? Any advice?

Clare J. Thornton, HTL(ASCP), QIHC
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthornton@dahlchase.com

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From shrivastavaanuradha <@t> hotmail.com  Wed Sep 14 21:35:12 2011
From: shrivastavaanuradha <@t> hotmail.com (anuradha shrivastava)
Date: Wed Sep 14 21:35:20 2011
Subject: [Histonet] H&E
Message-ID: 

Hello everybody,
Can anyone help me, In Leica autostainer we have after eosin 3 100% series, clear-rite/100% and then 3 clear rites. I observed that during humid days eosin was leaching . I replaced 2 clear rite after 50 :50
Abs/cler. with xylene. The slides were fine after wards. My question is , is it ok to keep the last clear rite or I should change that too to xylene. We had never used xylene before in the stainer. The coverslipper?s bath is filled with the clear rite.( xylene substitute), Please give your expert advice. Thanks .
From pathology01 <@t> rccltd.in  Wed Sep 14 22:47:36 2011
From: pathology01 <@t> rccltd.in (Pathology - RCC)
Date: Wed Sep 14 23:49:18 2011
Subject: [Histonet] Rep:Embedding centres
Message-ID: <000001cc735a$3729d100$a57d7300$@in>

Hi ,

 

I suggest to go for Leica or Thermo Shandon products.

 

Thanks & Regards,

S.Naveen Babu

Technician - Pathology

                

 

From Grant.Madill <@t> deedi.qld.gov.au  Thu Sep 15 01:22:13 2011
From: Grant.Madill <@t> deedi.qld.gov.au (Madill, Grant)
Date: Thu Sep 15 01:22:22 2011
Subject: [Histonet] Embedding Centre
Message-ID: <4A91E88A30D63B4EAA9E25EF05D984E6039C15FC@pibexhpr04.dpi.qld.gov.au>

We are looking at purchasing a Thermo Histostar.
What are your thoughts and experience with this embedder.

Cheers
Grant Madill
Laboratory Technician 
Animal Disease Surveillance Laboratory
Biosecurity Queensland 
Department of Employment, Economic Development and Innovation
203 Tor Street, Toowoomba Qld 4350

We're behind the Bid!
GOLD COAST 2018 - XXI COMMONWEALTH GAMES CANDIDATE CITY
www.goldcoast2018bid.com

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this communication in error, please notify the sender immediately 
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From tgenade <@t> gmail.com  Thu Sep 15 07:55:30 2011
From: tgenade <@t> gmail.com (Tyrone Genade)
Date: Thu Sep 15 07:55:39 2011
Subject: [Histonet] Re: Bouin's without the picric acid?
Message-ID: 

Thanks for all the replies.

Some alternatives or Bouin's substitutes have been suggested. Whether
they are OK with Bielschowsky?s and Gallyas Silver Staining and the
like remains to be determined. Whatever fixative I end up using must
be also OK with Thiolfavin-S & T, FluoroJadeB and
immunohistochemistry/fluorescence. My problem with PFA is that it can
destroy some epitoptes and some of my antibodies are directed against
sensitive epiptopes (glycoproteins).

I need an alternative fixative which isn't going to cause trouble for
a broaf range of staining techniques.

I have, with my whole mounts brains, used PFA for a 10 minute fix and
then 10 minute MeOH postfix without problems but 10 minutes is very
different to overnight..

Thanks for the advice. (John Kiernan, if you still out there, I would
love to hear from you!)
-- 
Tyrone Genade
http://tgenade.freeshell.org
email: tgenade@gmail.com
tel: +27-84-632-1925 (c)
********************************************************************************
Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord.
To find out how to receive this FREE gift visit http://www.alpha.org.

From sdysart <@t> mirnarx.com  Thu Sep 15 08:44:49 2011
From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com)
Date: Thu Sep 15 08:44:54 2011
Subject: [Histonet] H&E
In-Reply-To: 
References: 
Message-ID: 

This happened to me several months ago.  Our lab always has high humidity.  If you are going to continue to use clear rite with humidity in the air, I would change it everyday.  Also, change out your 100% because it's inevitably getting water in it too.  I just switched my whole lab back to xylene...at least you can see the water in it =)
Good Luck

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anuradha shrivastava
Sent: Wednesday, September 14, 2011 9:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H&E

Hello everybody,
Can anyone help me, In Leica autostainer we have after eosin 3 100% series, clear-rite/100% and then 3 clear rites. I observed that during humid days eosin was leaching . I replaced 2 clear rite after 50 :50
Abs/cler. with xylene. The slides were fine after wards. My question is , is it ok to keep the last clear rite or I should change that too to xylene. We had never used xylene before in the stainer. The coverslipper?s bath is filled with the clear rite.( xylene substitute), Please give your expert advice. Thanks .
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From campbellj <@t> muhlbauerlab.com  Thu Sep 15 08:59:34 2011
From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell)
Date: Thu Sep 15 08:59:40 2011
Subject: [Histonet] H&E
In-Reply-To: 
References: 
	
Message-ID: 

We use ProPar on our stainer. We rotate our 4 100% alcohols that are after
eosin after every 3rd rack and we have stopped the leaching of eosin in our
last ProPars.

Not a big fan of exposing my staff to xylene unless I absolutely have to.

Jen Campbell

On Thu, Sep 15, 2011 at 9:44 AM,  wrote:

> This happened to me several months ago.  Our lab always has high humidity.
>  If you are going to continue to use clear rite with humidity in the air, I
> would change it everyday.  Also, change out your 100% because it's
> inevitably getting water in it too.  I just switched my whole lab back to
> xylene...at least you can see the water in it =)
> Good Luck
>
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas  78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:
> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anuradha
> shrivastava
> Sent: Wednesday, September 14, 2011 9:35 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] H&E
>
> Hello everybody,
> Can anyone help me, In Leica autostainer we have after eosin 3 100% series,
> clear-rite/100% and then 3 clear rites. I observed that during humid days
> eosin was leaching . I replaced 2 clear rite after 50 :50
> Abs/cler. with xylene. The slides were fine after wards. My question is ,
> is it ok to keep the last clear rite or I should change that too to xylene.
> We had never used xylene before in the stainer. The coverslipper?s bath is
> filled with the clear rite.( xylene substitute), Please give your expert
> advice. Thanks .
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
Jen Campbell, HT(ASCP)
Supervisor of Technical Services
Muhlbauer Dermatopathology Laboratory
61 Monroe Avenue, Ste B
Pittsford NY 14534
P: 585.586.5166
F: 585.586.3137
From Barbara.Crill <@t> LPNT.net  Thu Sep 15 09:11:17 2011
From: Barbara.Crill <@t> LPNT.net (Barbara.Crill@LPNT.net)
Date: Thu Sep 15 09:11:28 2011
Subject: [Histonet] Lysol IC
Message-ID: <7DA79EBDBD92BF408EF392413737878D394BC42D3E@NADCWPMSGCMS01.hca.corpad.net>

I was told that the Lysol I.C. is being discontinued also.

Is anyone using Virex?











You can also use Lysol.I.C. or Virex to decontaminate the Ventana platforms





-----Original Message-----

Date: Thu, 25 Aug 2011 12:56:20

To: >

Subject: [Histonet] AMPHYL



My materials management department told me that AMPHYL has been discontinued.

Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL.



Has anyone else ran across this?  Has AMPHYL really been discontinued?

Is there a substitute we can use.



Thanks everyone!





ANTOINETTE CRILL

ANATOMIC PATHOLOGY

From MSHERWOOD <@t> PARTNERS.ORG  Thu Sep 15 09:11:38 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret)
Date: Thu Sep 15 09:11:46 2011
Subject: [Histonet] H&E
In-Reply-To: 
References: 
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB59DB@PHSXMB30.partners.org>

I don't know your specific protocol and what stains you are using (vendor), but
we switched a long time ago to CitriSolv, another xylene substitute and have had
not problems in our Leica Autostainer XL. We don't change the CitriSolv every
time and sometimes filter it.  


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anuradha
shrivastava
Sent: Wednesday, September 14, 2011 10:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H&E

Hello everybody,
Can anyone help me, In Leica autostainer we have after eosin 3 100% series,
clear-rite/100% and then 3 clear rites. I observed that during humid days eosin
was leaching . I replaced 2 clear rite after 50 :50
Abs/cler. with xylene. The slides were fine after wards. My question is , is it
ok to keep the last clear rite or I should change that too to xylene. We had
never used xylene before in the stainer. The coverslipper's bath is filled with
the clear rite.( xylene substitute), Please give your expert advice. Thanks .
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


From laurie.colbert <@t> huntingtonhospital.com  Thu Sep 15 09:37:25 2011
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Thu Sep 15 09:37:33 2011
Subject: [Histonet] Lysol IC
In-Reply-To: <7DA79EBDBD92BF408EF392413737878D394BC42D3E@NADCWPMSGCMS01.hca.corpad.net>
References: <7DA79EBDBD92BF408EF392413737878D394BC42D3E@NADCWPMSGCMS01.hca.corpad.net>
Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2B0E6@EXCHANGE3.huntingtonhospital.com>

I just received some Lysol IC from Lab Safety Supply.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Barbara.Crill@LPNT.net
Sent: Thursday, September 15, 2011 7:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Lysol IC

I was told that the Lysol I.C. is being discontinued also.

Is anyone using Virex?











You can also use Lysol.I.C. or Virex to decontaminate the Ventana
platforms





-----Original Message-----

Date: Thu, 25 Aug 2011 12:56:20

To:
>

Subject: [Histonet] AMPHYL



My materials management department told me that AMPHYL has been
discontinued.

Ventana recommends that we clean/decontaminate the Benchmark and the
Ultras with AMPHYL.



Has anyone else ran across this?  Has AMPHYL really been discontinued?

Is there a substitute we can use.



Thanks everyone!





ANTOINETTE CRILL

ANATOMIC PATHOLOGY

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From gu.lang <@t> gmx.at  Thu Sep 15 10:17:10 2011
From: gu.lang <@t> gmx.at (Gudrun Lang)
Date: Thu Sep 15 10:17:16 2011
Subject: [Histonet] warthin starry fading
Message-ID: 

Hi all!

Today I stained Warthin Starry for Hp. This time I was lucky to have the
first time purchased control tissue (Spirochetes control from Sigma) for
comparison.

In the package there was also a ready stained slide with really nice black
spirochetes in yellow-brownish tissue/nuclei. This was stained with a Sigma
Steiner Kit, but I thought my Warthin Starry should look the same.

So, after staining, the patient tissue shows nice Hp in yellow/brownish
background, but nuclei didn't stain. The control tissue had black-sprinkled
nuclei and no spirochetes at all. I was a little bit disappointed, but at
least the patient tissue was ok for diagnosis.

A few hours later I looked at the control slide again and found, that the
whole black staining had faded till a few speckles. (Patient slide was out
of reach to look at)

 

Does anybody know why this fading did happen? I have no information about
the fixation and processing of the control slides, but thought it must fit
for FFPET. The only point is, that we received the slides several months
before. Is aging a factor? 

 

Staining protocol in brief:

Everything diluted in pH 4 water, made with citric acid.

30 min 1% AgNO3

5-7 min developer (2% AgNO3+gelatine+Hydrochinon)

Waterrinse

Dehydration, coverslipping

 

Gudrun

From joycefr <@t> frontiernet.net  Thu Sep 15 11:47:27 2011
From: joycefr <@t> frontiernet.net (Joyce Friedland)
Date: Thu Sep 15 11:46:50 2011
Subject: [Histonet] H&E
Message-ID: <71C72728-0CEC-4CAC-A6A9-3CA145FFD721@frontiernet.net>

As I understand it, the Eosin is actually leached out by the ETOH that has been contaminated by water. The Propar that follows will show droplets of water in the bottom just as xylene does.

Joyce Friedland


"We can't control the wind but we can adjust our sails."


From gu.lang <@t> gmx.at  Thu Sep 15 13:11:07 2011
From: gu.lang <@t> gmx.at (Gudrun Lang)
Date: Thu Sep 15 13:11:12 2011
Subject: AW: [Histonet] warthin starry fading
In-Reply-To: 
References: 
Message-ID: 

I think I've found something. Obviously the staining result is sensitive for
special coverslipping medium. Patient slide was coverslipped with Pertex,
control slide was coverslipped with Eukit.
Interesting
Gudrun

-----Urspr?ngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun
Lang
Gesendet: Donnerstag, 15. September 2011 17:17
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] warthin starry fading

Hi all!

Today I stained Warthin Starry for Hp. This time I was lucky to have the
first time purchased control tissue (Spirochetes control from Sigma) for
comparison.

In the package there was also a ready stained slide with really nice black
spirochetes in yellow-brownish tissue/nuclei. This was stained with a Sigma
Steiner Kit, but I thought my Warthin Starry should look the same.

So, after staining, the patient tissue shows nice Hp in yellow/brownish
background, but nuclei didn't stain. The control tissue had black-sprinkled
nuclei and no spirochetes at all. I was a little bit disappointed, but at
least the patient tissue was ok for diagnosis.

A few hours later I looked at the control slide again and found, that the
whole black staining had faded till a few speckles. (Patient slide was out
of reach to look at)

 

Does anybody know why this fading did happen? I have no information about
the fixation and processing of the control slides, but thought it must fit
for FFPET. The only point is, that we received the slides several months
before. Is aging a factor? 

 

Staining protocol in brief:

Everything diluted in pH 4 water, made with citric acid.

30 min 1% AgNO3

5-7 min developer (2% AgNO3+gelatine+Hydrochinon)

Waterrinse

Dehydration, coverslipping

 

Gudrun

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From b-frederick <@t> northwestern.edu  Thu Sep 15 13:46:51 2011
From: b-frederick <@t> northwestern.edu (Bernice Frederick)
Date: Thu Sep 15 13:47:18 2011
Subject: [Histonet] Phosphogard/guard
Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E13FAD1@evcspmbx3.ads.northwestern.edu>

Has anyone heard of or used this for protection of phosphorylation sites during IHC? My manager wants to try it.
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick@northwestern.edu

From rsrichmond <@t> gmail.com  Thu Sep 15 13:53:14 2011
From: rsrichmond <@t> gmail.com (Bob Richmond)
Date: Thu Sep 15 13:53:18 2011
Subject: [Histonet] Re: Bouin's without picric acid
Message-ID: 

Davidson's fixative is made of three parts of tap water, three parts
alcohol (you can use waste processing alcohol), two parts 37% formalin
(not neutral buffered formalin) and one part glacial acetic acid.

I have references if anyone wants them - they're pretty obscure.

Bob Richmond
Samurai Pathologist
Knoxville TN
****************************************************
Tyrone -
I have been working with fish (whole and pieces and parts) for years.  The
fixative of choice  for whole fish as been Davidson's.  It contains
formalin, alcohol, acetic acid and water.

Cheryl Crowder, BA, HTL(ASCP)
Crowder Histology Consulting
4952 Alvin Dark Ave.
Baton Rouge, LA  70820

From Laura.Miller <@t> leica-microsystems.com  Thu Sep 15 14:34:48 2011
From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com)
Date: Thu Sep 15 14:34:55 2011
Subject: [Histonet] Laura Miller is Out of  the Office.
Message-ID: 


I will be out of the office starting  09/15/2011 and will not return until
09/22/2011.

I am attending the National Society of Histotechnology meeting in
Cincinnati, OH.  I will have limited access to email.  I will respond to
your message as soon as possible.


______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
______________________________________________________________________

From sdysart <@t> mirnarx.com  Thu Sep 15 14:40:00 2011
From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com)
Date: Thu Sep 15 14:40:04 2011
Subject: [Histonet] IHC ?
Message-ID: 

Hello all,

So I just realized I am out of my block after I have done HIER.  Can I
leave the slides in buffer in the fridge or something overnight.  The
block will be here at 10am tomorrow morning...

She the slides be ok or do I need to re-cut all of them and start over
(please tell me this is not the case)

=)

 

Sarah Goebel-Dysart, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

From chapcl <@t> yahoo.com  Thu Sep 15 14:43:19 2011
From: chapcl <@t> yahoo.com (William Chappell)
Date: Thu Sep 15 14:43:22 2011
Subject: [Histonet] IHC ?
In-Reply-To: 
References: 
Message-ID: <5DC7D426-E194-49E9-A948-A9E33DDC9830@yahoo.com>

Excellent questions, I get it all the time.  Overnight, in buffer, in the fridge will be ok.  Do not leave in water as that will obscure morphology.

Will


On Sep 15, 2011, at 12:40 PM,  wrote:

> Hello all,
> 
> So I just realized I am out of my block after I have done HIER.  Can I
> leave the slides in buffer in the fridge or something overnight.  The
> block will be here at 10am tomorrow morning...
> 
> She the slides be ok or do I need to re-cut all of them and start over
> (please tell me this is not the case)
> 
> =)
> 
> 
> 
> Sarah Goebel-Dysart, BA, HT(ASCP)
> 
> Histotechnologist
> 
> Mirna Therapeutics
> 
> 2150 Woodward Street
> 
> Suite 100
> 
> Austin, Texas  78744
> 
> (512)901-0900 ext. 6912
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From rjbuesa <@t> yahoo.com  Thu Sep 15 15:35:54 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Sep 15 15:35:57 2011
Subject: [Histonet] IHC ?
In-Reply-To: 
Message-ID: <1316118954.40850.YahooMailClassic@web65703.mail.ac4.yahoo.com>

I have left them overnight in PBS in the refrigerator without any problem. It is always better to do that and maybe replace a few that the whole bunch of slides.
Ren? J.

--- On Thu, 9/15/11, sdysart@mirnarx.com  wrote:


From: sdysart@mirnarx.com 
Subject: [Histonet] IHC ?
To: histonet@lists.utsouthwestern.edu
Date: Thursday, September 15, 2011, 3:40 PM


Hello all,

So I just realized I am out of my block after I have done HIER.? Can I
leave the slides in buffer in the fridge or something overnight.? The
block will be here at 10am tomorrow morning...

She the slides be ok or do I need to re-cut all of them and start over
(please tell me this is not the case)

=)



Sarah Goebel-Dysart, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas? 78744

(512)901-0900 ext. 6912



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From shrivastavaanuradha <@t> hotmail.com  Thu Sep 15 20:37:13 2011
From: shrivastavaanuradha <@t> hotmail.com (anuradha shrivastava)
Date: Thu Sep 15 20:37:17 2011
Subject: [Histonet] Thanks 
Message-ID: 

Hello Histogurus,
Thank you very much for valuable advice.
Anu.
From Grant.Madill <@t> deedi.qld.gov.au  Fri Sep 16 00:03:21 2011
From: Grant.Madill <@t> deedi.qld.gov.au (Madill, Grant)
Date: Fri Sep 16 00:03:26 2011
Subject: [Histonet] Warthin starry fading
Message-ID: <4A91E88A30D63B4EAA9E25EF05D984E6039C1F0A@pibexhpr04.dpi.qld.gov.au>

Hi Gudrun
I had this same problem some years back. I changed from a previous mounting medium to Merck Entellan New.
The H&E's were fine but when I did warthin-starry staining they were completely faded the next day.
I thought it must be the new mounting medium so I bought some Pertex and ran 2 control slides using each
mounting medium and it was obvious that the Entellen was at fault. Only use Pertex now and find it very good.

Cheers
Grant Madill
Laboratory Technician 
Animal Disease Surveillance Laboratory
Biosecurity Queensland 
Department of Employment, Economic Development and Innovation
203 Tor Street, Toowoomba Qld 4350
*  PO Box 102, Toowoomba Qld 4350
*  +61 7 4688 1363
*   +61 7 4688 1195
*    grant.madill@deedi.qld.gov.au  

Business Information Centre 13 25 23
www.deedi.qld.gov.au   


We're behind the Bid!
GOLD COAST 2018 - XXI COMMONWEALTH GAMES CANDIDATE CITY
www.goldcoast2018bid.com

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From charleso.o606 <@t> gmail.com  Fri Sep 16 04:46:55 2011
From: charleso.o606 <@t> gmail.com (Charles O)
Date: Fri Sep 16 04:47:01 2011
Subject: [Histonet] Seeking new position
In-Reply-To: 
References: 
Message-ID: 

   Hi,
      I am a certified histotech ( with both HT (ASC) and  HTL (ASCP) Plus
QIHC (ASCP) seeking a new position. I have an extensive experience in the
general histological technics and in Immunohistochemistry. I am willing to
relocate.

    Thanks,

  Charles O.
From Carmen.M.Garcia <@t> uv.es  Fri Sep 16 05:47:28 2011
From: Carmen.M.Garcia <@t> uv.es (Carmen Maria Garcia Pascual)
Date: Fri Sep 16 05:47:37 2011
Subject: [Histonet] H&M staining
In-Reply-To: 
References: 
Message-ID: <2917592559carmaga6@uv.es>


Good morning:

I have a question, I want to know what can I do if I get a very strong 
staining... it's reversible?

Thank you very much!
Best regards,

Carmen


From tshrobertson <@t> yahoo.com  Fri Sep 16 07:38:59 2011
From: tshrobertson <@t> yahoo.com (Teisha Robertson)
Date: Fri Sep 16 07:39:03 2011
Subject: [Histonet] (no subject)
Message-ID: <1316176739.45696.YahooMailMobile@web126002.mail.ne1.yahoo.com>

http://designkrby.cz/oldtemp/arccre.htm
From dreynold <@t> mdanderson.org  Fri Sep 16 08:04:37 2011
From: dreynold <@t> mdanderson.org (Reynolds,Donna M)
Date: Fri Sep 16 08:03:22 2011
Subject: [Histonet] Manual IHC staining
Message-ID: <785BBF0C5F49CE41BA74460A43A08F022FD72576C5@DCPWVMBXC0VS3.mdanderson.edu>

We have a system we make that uses Plexiglas  boxes to create the humid environment and a special holder inside that holds  1-10 slides that can be picked up and washed all at once.
We have our new baby chamber that only holds 5 slides for the minnie jobs. Our chambers will hold 3 holders =30 slides, 4 holders =40 slides, and 6 holders =60 slides. Each slide can be treated individually so variations are almost endless just how adventurous you fell. \
Any one interested in these can contact me at my personal e-mail. cdr186@hotmail.com and I can supply more information.

Donna Reynolds  (HT)ASCP
281-954-5022

From BMolinari <@t> heart.thi.tmc.edu  Fri Sep 16 08:56:02 2011
From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy)
Date: Fri Sep 16 08:56:29 2011
Subject: [Histonet] Von Kossa vs Alizarin Red
Message-ID: 

Hi,
 Under what circumstances would you prefer one over the other? Thanks! Happy Friday! I hope all you convention goers ( is that even a word??) are having a great time!

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
MC1-283
Houston,TX 77030-2607
832-355-6524
832-355-6812

From ree3 <@t> leicester.ac.uk  Fri Sep 16 09:30:50 2011
From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.)
Date: Fri Sep 16 09:31:21 2011
Subject: [Histonet] Sequenza
In-Reply-To: 
References: <953560.67364.qm@web112619.mail.gq1.yahoo.com>
	
Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4EC22F759@EXC-MBX3.cfs.le.ac.uk>

According to my source at Fisher Scientific U.K, the  distributor for the  Shandon range in the  U.K., the Sequenza   and perhaps more importantly, the  Coverplates are still listed and available.

                                             Richard Edwards

                                                     Leicester U.K.

From Judith_Pardue <@t> memorial.org  Fri Sep 16 10:22:22 2011
From: Judith_Pardue <@t> memorial.org (Pardue, Judith)
Date: Fri Sep 16 10:31:03 2011
Subject: [Histonet] Leica Bond verses the Ventana Ultra
Message-ID: <14B823F24E628E49BBFAD704E4BAB89A0F19D6@chimsx03.CHI.catholichealth.net>

Need to know histotechs opinion of the Leica Bond verses the Ventana
Ultra. 
 
judith_pardue@memorial.org
 
Judith Pardue




This message and accompanying documents are covered by
the Electronic Communications Privacy Act 18
U.S.C. "Sections 2510-2521," and contain information
intended for the specified individual(s) only. This
information is confidential.  If you are not the intended
recipient or an agent responsible for delivering it to
the intended recipient, you are hereby notified that you
have received this document in error and that any review,
dissemination, copying, or the taking of any action based
on the contents of this information is strictly
prohibited.  If you have received this communication in
error, please notify us immediately by e-mail, and delete
the original message.
 


From JCBRITTON <@t> Cheshire-Med.COM  Fri Sep 16 10:37:14 2011
From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C)
Date: Fri Sep 16 10:37:17 2011
Subject: [Histonet] Leica Bond verses the Ventana Ultra
In-Reply-To: <14B823F24E628E49BBFAD704E4BAB89A0F19D6@chimsx03.CHI.catholichealth.net>
References: <14B823F24E628E49BBFAD704E4BAB89A0F19D6@chimsx03.CHI.catholichealth.net>
Message-ID: 

I love the Bond!

 

Josie Britton HT

Cheshire Medical Center 

Keene, NH

 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue,
Judith
Sent: Friday, September 16, 2011 11:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond verses the Ventana Ultra

 

Need to know histotechs opinion of the Leica Bond verses the Ventana

Ultra. 

 

judith_pardue@memorial.org

 

Judith Pardue

 

 

 

 

This message and accompanying documents are covered by

the Electronic Communications Privacy Act 18

U.S.C. "Sections 2510-2521," and contain information

intended for the specified individual(s) only. This

information is confidential.  If you are not the intended

recipient or an agent responsible for delivering it to

the intended recipient, you are hereby notified that you

have received this document in error and that any review,

dissemination, copying, or the taking of any action based

on the contents of this information is strictly

prohibited.  If you have received this communication in

error, please notify us immediately by e-mail, and delete

the original message.

 

 

 

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From HornHV <@t> archildrens.org  Fri Sep 16 11:28:40 2011
From: HornHV <@t> archildrens.org (Horn, Hazel V)
Date: Fri Sep 16 11:28:43 2011
Subject: [Histonet] RE: Leica Bond verses the Ventana Ultra
In-Reply-To: <14B823F24E628E49BBFAD704E4BAB89A0F19D6@chimsx03.CHI.catholichealth.net>
References: <14B823F24E628E49BBFAD704E4BAB89A0F19D6@chimsx03.CHI.catholichealth.net>
Message-ID: <25A4DE08332B19499904459F00AAACB719A93F383C@EVS1.archildrens.org>

We love our Bond too.  

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith
Sent: Friday, September 16, 2011 10:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond verses the Ventana Ultra

Need to know histotechs opinion of the Leica Bond verses the Ventana
Ultra. 
 
judith_pardue@memorial.org
 
Judith Pardue




This message and accompanying documents are covered by
the Electronic Communications Privacy Act 18
U.S.C. "Sections 2510-2521," and contain information
intended for the specified individual(s) only. This
information is confidential.  If you are not the intended
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error, please notify us immediately by e-mail, and delete
the original message.
 


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From trathborne <@t> somerset-healthcare.com  Fri Sep 16 11:33:03 2011
From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni)
Date: Fri Sep 16 11:33:14 2011
Subject: [Histonet] RE: Leica Bond verses the Ventana Ultra
In-Reply-To: <25A4DE08332B19499904459F00AAACB719A93F383C@EVS1.archildrens.org>
References: <14B823F24E628E49BBFAD704E4BAB89A0F19D6@chimsx03.CHI.catholichealth.net>
	<25A4DE08332B19499904459F00AAACB719A93F383C@EVS1.archildrens.org>
Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F56B4F@SMCMAIL01.somerset-healthcare.com>

We love our Bond, but can not compare to the Ultra for long-term use. We demo'd both instruments, and chose the Bond.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Friday, September 16, 2011 12:29 PM
To: 'Pardue, Judith'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Leica Bond verses the Ventana Ultra

We love our Bond too.  

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith
Sent: Friday, September 16, 2011 10:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond verses the Ventana Ultra

Need to know histotechs opinion of the Leica Bond verses the Ventana Ultra. 
 
judith_pardue@memorial.org
 
Judith Pardue




This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential.  If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited.  If you have received this communication in error, please notify us immediately by e-mail, and delete the original message.
 


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From rjbuesa <@t> yahoo.com  Fri Sep 16 11:50:56 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Fri Sep 16 11:50:59 2011
Subject: [Histonet] Manual IHC staining
In-Reply-To: <785BBF0C5F49CE41BA74460A43A08F022FD72576C5@DCPWVMBXC0VS3.mdanderson.edu>
Message-ID: <1316191856.15356.YahooMailClassic@web65707.mail.ac4.yahoo.com>

Under separate cover I am sending a photo of our large humid chamber
Ren? J.

--- On Fri, 9/16/11, Reynolds,Donna M  wrote:


From: Reynolds,Donna M 
Subject: [Histonet] Manual IHC staining
To: "'histonet@lists.utsouthwestern.edu'" 
Date: Friday, September 16, 2011, 9:04 AM


We have a system we make that uses Plexiglas? boxes to create the humid environment and a special holder inside that holds? 1-10 slides that can be picked up and washed all at once.
We have our new baby chamber that only holds 5 slides for the minnie jobs. Our chambers will hold 3 holders =30 slides, 4 holders =40 slides, and 6 holders =60 slides. Each slide can be treated individually so variations are almost endless just how adventurous you fell. \
Any one interested in these can contact me at my personal e-mail. cdr186@hotmail.com and I can supply more information.

Donna Reynolds? (HT)ASCP
281-954-5022

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From CIngles <@t> uwhealth.org  Fri Sep 16 12:36:53 2011
From: CIngles <@t> uwhealth.org (Ingles Claire )
Date: Fri Sep 16 12:38:58 2011
Subject: [Histonet] RE: Leica Bond verses the Ventana Ultra
References: <14B823F24E628E49BBFAD704E4BAB89A0F19D6@chimsx03.CHI.catholichealth.net><25A4DE08332B19499904459F00AAACB719A93F383C@EVS1.archildrens.org>
	<3AD061FE740D464FAC7BF6B5CFB7570711F56B4F@SMCMAIL01.somerset-healthcare.com>
Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A768@UWHC-MAIL2.uwhis.hosp.wisc.edu>

James Bond? Sean Connery was always my favorite. :) Happy Friday! I'm off for a week and a half to the northwoods. Those fish better look out, and I don't mean zebrafish.
Claire

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rathborne, Toni
Sent: Fri 9/16/2011 11:33 AM
To: 'Horn, Hazel V'; 'Pardue, Judith'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Leica Bond verses the Ventana Ultra



We love our Bond, but can not compare to the Ultra for long-term use. We demo'd both instruments, and chose the Bond.




From brian <@t> prometheushealthcare.com  Fri Sep 16 12:47:51 2011
From: brian <@t> prometheushealthcare.com (Brian- Prometheus)
Date: Fri Sep 16 12:48:01 2011
Subject: [Histonet] Histology Opening in Central NJ
Message-ID: <006801cc7498$c4f93860$4eeba920$@com>

Reference lab located in Eatontown, NJ currently searching for a night
histotech.

 

mostly doing general histology

 

will train

 

hours roughly 9pm to 5:30am

 

Please contact me today for immediate consideration.

 

Brian Feldman

Principal

Prometheus Healthcare 

Office 301-693-9057

Fax 301-368-2478

 
 brian@prometheushealthcare.com

  www.prometheushealthcare.com

*** Stay up to date on the newest positions and healthcare trends nationwide
on Twitter!***

   http://twitter.com/PrometheusBlog

 

 

 

 

 

 

From TJJ <@t> stowers.org  Fri Sep 16 12:50:28 2011
From: TJJ <@t> stowers.org (Johnson, Teri)
Date: Fri Sep 16 12:50:33 2011
Subject: [Histonet] Re: Von Kossa vs Alizarin Red
Message-ID: <2C40E43D1F7A56408C4463FD245DDDF98420362C@EXCHMB-02.stowers-institute.org>

Hi Betsy,

How timely is your question! We are (well, actually Nancy Thomas is) currently evaluating the two stains on some chameleon heads and we have found something really interesting we never considered. The chameleon has quite a bit of melanin pigment in places. The fact the von kossa will end up staining the bone black, it doesn't contrast well with the areas of pigment throughout the connective tissue. Otherwise, in other species I usually prefer the von kossa.

The silver technique might also work better for combined stains or combined histochemical and immunohistochemical staining than using the Alizarin Red. Just my preference.

~Teri

From amosbrooks <@t> gmail.com  Fri Sep 16 17:15:27 2011
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Fri Sep 16 17:15:32 2011
Subject: [Histonet] Von Kossa vs Alizarin Red
Message-ID: 

Hi,
    I do both often. You would want the VonKossa rather than Alizarin Red if
you had any plans to do any subsequent double staining. The silver isn't
going anywhere. The Alizarin Red will disappear as soon as you place the
slides in water (or alcohol) so you can't stain anything else. Since the
silver is insoluble you can do dang near anything to it and it will still
remain.

Amos


On Fri, Sep 16, 2011 at 1:01 PM,
wrote:

> Message: 14
> Date: Fri, 16 Sep 2011 13:56:02 +0000
> From: "Molinari, Betsy" 
> Subject: [Histonet] Von Kossa vs Alizarin Red
> To: "'histonet@lists.utsouthwestern.edu'"
>        
> Message-ID:
>        
> Content-Type: text/plain; charset="us-ascii"
>
> Hi,
>  Under what circumstances would you prefer one over the other? Thanks!
> Happy Friday! I hope all you convention goers ( is that even a word??) are
> having a great time!
>
> Betsy Molinari HT(ASCP)
> Texas Heart Institute
> Cardiovascular Pathology
> 6770 Bertner Ave
> MC1-283
> Houston,TX 77030-2607
> 832-355-6524
> 832-355-6812
>
From minhan.science <@t> gmail.com  Fri Sep 16 22:33:29 2011
From: minhan.science <@t> gmail.com (Min-Han Tan)
Date: Fri Sep 16 22:33:32 2011
Subject: [Histonet] Question of combining immunofluorescence and more
 conventional cytologic stains
Message-ID: 

Dear all,

Apologies for the trouble. I am working on fresh human cancer cells on
slides here in Singapore. I am trying to visualize the slides concurrently
using both immunofluorescence and a more conventional nuclear stain used in
pathology laboratories.

I have tried Diffquik and Giemsa, but both seem to quench the fluorescence
signal. I have tried a GFP-expressing cell line, and the same quenching
seems to occur as well.

I was wondering if anyone has ever needed to do similar work, and if so,
what sort of solutions (bad pun) were adopted?

Thank you!

Min-Han
From tahseen <@t> brain.net.pk  Fri Sep 16 23:35:34 2011
From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk)
Date: Fri Sep 16 23:35:41 2011
Subject: [Histonet] Leica Bond verses the Ventana Ultra
In-Reply-To: <14B823F24E628E49BBFAD704E4BAB89A0F19D6@chimsx03.CHI.catholichealth.ne
	t>
References: <14B823F24E628E49BBFAD704E4BAB89A0F19D6@chimsx03.CHI.catholichealth.net>
Message-ID: <54083.203.135.35.66.1316234134.squirrel@brain.net.pk>

We love our Bond too.
Muhammad Tahseen
Histology supervisor
SKMCH & RC
Lahore Pakistan

> Need to know histotechs opinion of the Leica Bond verses the Ventana
> Ultra.
>
> judith_pardue@memorial.org
>
> Judith Pardue
>
>
>
>
> This message and accompanying documents are covered by
> the Electronic Communications Privacy Act 18
> U.S.C. "Sections 2510-2521," and contain information
> intended for the specified individual(s) only. This
> information is confidential.  If you are not the intended
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> the intended recipient, you are hereby notified that you
> have received this document in error and that any review,
> dissemination, copying, or the taking of any action based
> on the contents of this information is strictly
> prohibited.  If you have received this communication in
> error, please notify us immediately by e-mail, and delete
> the original message.
>
>
>
> _______________________________________________
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



From tshrobertson <@t> yahoo.com  Sat Sep 17 04:33:47 2011
From: tshrobertson <@t> yahoo.com (Teisha Robertson)
Date: Sat Sep 17 04:33:51 2011
Subject: [Histonet] (no subject)
Message-ID: <1316252027.96051.YahooMailMobile@web126003.mail.ne1.yahoo.com>

http://kaniando.com/cpg15/albums/userpics/clreefg.htm
From rgeske_2000 <@t> yahoo.com  Sat Sep 17 05:54:30 2011
From: rgeske_2000 <@t> yahoo.com (Rob Geske)
Date: Sat Sep 17 05:54:34 2011
Subject: [Histonet] Von Kossa vs Alizarin Red
In-Reply-To: 
References: 
Message-ID: <1316256870.95018.YahooMailNeo@web39421.mail.mud.yahoo.com>

Hi Betsy,

depends on the intent of the experiment.? if goal is to determine calcification? (calcium or hydroxyapatite) then Alizarin Red. as Von Kossa is a reaction between silver ions and the associated phosphate you cannot definitively claim calcification but rather mineralization.? hope all is well in THI.

regards,
rob



________________________________
From: "Molinari, Betsy" 
To: "'histonet@lists.utsouthwestern.edu'" 
Sent: Friday, September 16, 2011 9:56 AM
Subject: [Histonet] Von Kossa vs Alizarin Red

Hi,
Under what circumstances would you prefer one over the other? Thanks! Happy Friday! I hope all you convention goers ( is that even a word??) are having a great time!

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
MC1-283
Houston,TX 77030-2607
832-355-6524
832-355-6812

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From SteveM <@t> mcclainlab.com  Sat Sep 17 16:45:55 2011
From: SteveM <@t> mcclainlab.com (Steve McClain)
Date: Sat Sep 17 16:33:09 2011
Subject: [Histonet] RE: Histonet Digest, Vol 94,
	Issue 20 Question of combining immunofluorescence and more
Message-ID: 

Dual Fluorescence may give you a start.
You can try Hoescht stain for nuclei and use another wavelength for the
second signal.

Eosin fluoresces , except eosin does not stain nuclei and being related
to fluoroscein eosin works on the green wavelength.

Steve
Steve A. McClain, MD
McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000

From tshrobertson <@t> yahoo.com  Sat Sep 17 19:44:02 2011
From: tshrobertson <@t> yahoo.com (Teisha Robertson)
Date: Sat Sep 17 19:44:06 2011
Subject: [Histonet] (no subject)
Message-ID: <1316306642.97714.YahooMailMobile@web126003.mail.ne1.yahoo.com>

http://www.dynanavblog.com/wp-content/themes/wootube/arccre.htm
From amosbrooks <@t> gmail.com  Sat Sep 17 20:13:59 2011
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Sat Sep 17 20:14:04 2011
Subject: [Histonet] Question of combining immunofluorescence and more
	conventional cytologic stains
Message-ID: 

Hi,
     Have you tried doing the conventional stain first, then the fluorescent
after. As long as the epitope survives the initial staining, it should be
fairly easy to label the cells with a fluorescent tag.

Amos

On Sat, Sep 17, 2011 at 1:00 PM,
wrote:

> Message: 5
> Date: Sat, 17 Sep 2011 11:33:29 +0800
> From: Min-Han Tan 
> Subject: [Histonet] Question of combining immunofluorescence and more
>        conventional cytologic stains
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
>         >
> Content-Type: text/plain; charset=ISO-8859-1
>
> Dear all,
>
> Apologies for the trouble. I am working on fresh human cancer cells on
> slides here in Singapore. I am trying to visualize the slides concurrently
> using both immunofluorescence and a more conventional nuclear stain used in
> pathology laboratories.
>
> I have tried Diffquik and Giemsa, but both seem to quench the fluorescence
> signal. I have tried a GFP-expressing cell line, and the same quenching
> seems to occur as well.
>
> I was wondering if anyone has ever needed to do similar work, and if so,
> what sort of solutions (bad pun) were adopted?
>
> Thank you!
>
> Min-Han
>
From Melissa.Mazan <@t> tufts.edu  Sun Sep 18 16:23:25 2011
From: Melissa.Mazan <@t> tufts.edu (Mazan, Melissa)
Date: Sun Sep 18 16:24:23 2011
Subject: [Histonet] combining immunofluorescence and conventional
Message-ID: <5E743323406EA847A2B1B38A3A2FF5CC08685596@TFTMEXDAG01MB03.tufts.ad.tufts.edu>

Hi all, 
You have to do the fluorescence last.  Almost everything else will quench the signal.  Melissa

Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor, Director of Equine Sports Medicine
Department of Clinical Sciences
Cummings School of Veterinary Medicine
Tufts University
200 Westborough Road
North Grafton, MA, 01536
tel: 508-839-5395
email: melissa.mazan@tufts.edu

________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu]
Sent: Sunday, September 18, 2011 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 94, Issue 21

Send Histonet mailing list submissions to
        histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
        http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. RE: Histonet Digest, Vol 94,      Issue 20 Question of combining
      immunofluorescence and more (Steve McClain)
   2. (no subject) (Teisha Robertson)
   3. Question of combining immunofluorescence and more
      conventional cytologic stains (Amos Brooks)


----------------------------------------------------------------------

Message: 1
Date: Sat, 17 Sep 2011 17:45:55 -0400
From: "Steve McClain" 
Subject: [Histonet] RE: Histonet Digest, Vol 94,        Issue 20 Question of
        combining immunofluorescence and more
To: 
Message-ID:
        
Content-Type: text/plain;       charset="us-ascii"

Dual Fluorescence may give you a start.
You can try Hoescht stain for nuclei and use another wavelength for the
second signal.

Eosin fluoresces , except eosin does not stain nuclei and being related
to fluoroscein eosin works on the green wavelength.

Steve
Steve A. McClain, MD
McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000



------------------------------

Message: 2
Date: Sat, 17 Sep 2011 17:44:02 -0700 (PDT)
From: Teisha Robertson 
Subject: [Histonet] (no subject)
To: deanl@jackson.org, deanl@jacksonhospital.net,
        huttowa@kellyservices.com,      histonet@lists.utsouthwestern.edu
Message-ID:
        <1316306642.97714.YahooMailMobile@web126003.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=us-ascii

http://www.dynanavblog.com/wp-content/themes/wootube/arccre.htm

------------------------------

Message: 3
Date: Sat, 17 Sep 2011 21:13:59 -0400
From: Amos Brooks 
Subject: [Histonet] Question of combining immunofluorescence and more
        conventional cytologic stains
To: histonet@lists.utsouthwestern.edu, minhan.science@gmail.com
Message-ID:
        
Content-Type: text/plain; charset=ISO-8859-1

Hi,
     Have you tried doing the conventional stain first, then the fluorescent
after. As long as the epitope survives the initial staining, it should be
fairly easy to label the cells with a fluorescent tag.

Amos

On Sat, Sep 17, 2011 at 1:00 PM,
wrote:

> Message: 5
> Date: Sat, 17 Sep 2011 11:33:29 +0800
> From: Min-Han Tan 
> Subject: [Histonet] Question of combining immunofluorescence and more
>        conventional cytologic stains
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
>         >
> Content-Type: text/plain; charset=ISO-8859-1
>
> Dear all,
>
> Apologies for the trouble. I am working on fresh human cancer cells on
> slides here in Singapore. I am trying to visualize the slides concurrently
> using both immunofluorescence and a more conventional nuclear stain used in
> pathology laboratories.
>
> I have tried Diffquik and Giemsa, but both seem to quench the fluorescence
> signal. I have tried a GFP-expressing cell line, and the same quenching
> seems to occur as well.
>
> I was wondering if anyone has ever needed to do similar work, and if so,
> what sort of solutions (bad pun) were adopted?
>
> Thank you!
>
> Min-Han
>


------------------------------

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 94, Issue 21
****************************************
From tgenade <@t> gmail.com  Mon Sep 19 07:49:24 2011
From: tgenade <@t> gmail.com (Tyrone Genade)
Date: Mon Sep 19 07:49:34 2011
Subject: [Histonet] Re: Bouin's without picric acid
Message-ID: 

Thanks to those of you who commented on my picric acid-less Bouin's
problem. The solution has been found: Davidson's.

Thanks for the help.
-- 
Tyrone Genade
http://tgenade.freeshell.org
email: tgenade@gmail.com
tel: +27-84-632-1925 (c)
********************************************************************************
Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord.
To find out how to receive this FREE gift visit http://www.alpha.org.

From Kim.Kolman <@t> va.gov  Mon Sep 19 08:20:17 2011
From: Kim.Kolman <@t> va.gov (Kolman, Kimberly D.)
Date: Mon Sep 19 08:20:32 2011
Subject: [Histonet] jpc and broken slides
Message-ID: <9C32F30B6662D74A8419DDDB7E66656A065F7087@VHAV15MSGA1.v15.med.va.gov>

I'm sure I missed some of the talk on the 'afip closing' thread' but has
anyone mentioned a NEW problem with broken slides on JPC consult cases?


 

For those of you able to use JPC for consults: 

 

All of the sudden, there have been a number of cases that our lab has
sent to JPC that they've called and complained that some slides were
broken (they're claiming upon receipt).

 

We've been packaging them the very same way as when they were sent to
AFIP.  Not sure what else we could do.  

 

 

 

Kimberly D. Kolman, HT, (ASCP)

Diagnostics 115 

VA Eastern Kansas Health Care System

4101 S. 4th St. Trfwy.

Leavenworth, KS 66048

ph: 913-682-2000 x 52537/52539

 

 

From Gary_Steinke <@t> vwr.com  Mon Sep 19 13:02:34 2011
From: Gary_Steinke <@t> vwr.com (Gary_Steinke@vwr.com)
Date: Mon Sep 19 13:02:42 2011
Subject: [Histonet] Gary Steinke is out of the office
Message-ID: 


I will be out of the office starting  09/19/2011 and will not return until
09/26/2011.

I will be out of the office from September 19th and returning on September
26th.  I will out of town attending a business meeting and will have very
limited access to email and voice mail.  If you need assistance
immediately, please contact Customer Service at 877-881-1192.  Take care
and have a great day.


From Ken_Marissael <@t> vwr.com  Mon Sep 19 13:03:30 2011
From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com)
Date: Mon Sep 19 13:03:39 2011
Subject: [Histonet] Ken Marissael is out of the office
Message-ID: 


I will be out of the office starting  09/19/2011 and will not return until
09/23/2011.

I will be away on 9/16 and return 9/27. I will have limited e-mail and cell
phone access, but will try to get back to you as quickly as possible. While
I am away, please contact VWR Healthcare Customer Service at 877-881-1192.


From Joyce.Cline <@t> meritushealth.com  Mon Sep 19 13:19:16 2011
From: Joyce.Cline <@t> meritushealth.com (Joyce Cline)
Date: Mon Sep 19 13:21:23 2011
Subject: [Histonet] Histotech needed
Message-ID: 

Full time position, M-F varying shifts 6:00am to 6:00pm, rotating bench assignments, IHC, special stains and autopsies. No Saturdays or week end call.

We are near Wash D.C., Baltimore and other site seeing areas.

Go to Meritushealth.com HR department for job listings.

Joyce Cline, H. T. (ASCP)
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
joyce.cline@meritushealth.com

________________________________
***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system.
From Diane.Tokugawa <@t> kp.org  Mon Sep 19 18:25:31 2011
From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org)
Date: Mon Sep 19 18:30:07 2011
Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.
Message-ID: 


I will be out of the office starting  09/19/2011 and will not return until
09/21/2011.

Note:   For Cytology issues, please call Molly  at 8-421-5487,  Eric at
8-421-5405, or Wanda 8-421-5426   For Histology issues, please call Mario
at 8-421-4961, general histology lab 8-421- 5408 or Wanda at 8-421-5426.
From itai.moshe <@t> mail.huji.ac.il  Tue Sep 20 01:23:04 2011
From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe)
Date: Tue Sep 20 01:23:09 2011
Subject: [Histonet] How to store paraffin blocks ?
Message-ID: 

Hi Histonet's,

What is the recommended way to store paraffin blocks for a long period ?
Room temperature, or 4C ?

The advantage in storing at 4C is that the blocks are always ready for
sectioning.
Is there a risk when storing at 4C that the humidity in the fridge will
cause damage to the paraffin blocks and the tissue ?
After sectioning, How do you store the slides, and for how long ?


Thank's
Itai Moshe
From Gudrun.Erikstad <@t> stolav.no  Tue Sep 20 01:55:28 2011
From: Gudrun.Erikstad <@t> stolav.no (Erikstad, Gudrun Hovstein)
Date: Tue Sep 20 01:55:37 2011
Subject: SV: [Histonet] How to store paraffin blocks ?
In-Reply-To: 
Message-ID: 


 Hi,

We store paraffin blocks and stained slides at room temperature in rooms without windows, so there is no sunlight.
We store blocks and slides "forever". We have paraffin blocks from before 1930.
"Room temperature" in Norway is usually about 20 degrees Celcius.

Some stained slides will fade with time (silver techniques, immuno), but the routine HE usually last for many, many years.
After we excanged Xylene with a substitute (Tissue-Clear) four years ago, we had some "bleeding" and fading of the HE-stains, but after taking more care changing the Xylene substitute in the clearing step more often this is no more a problem.
The paraffin blocks keep very well. We frequently do section and stain as well as do molecular techniques (PCR, In situ) on material from the 1970'ies and before, and it's remarkable how well the formalin fixed, paraffin embedded tissue keep.

I would not keep the blocks in a fridge, as you say, the humidity in the fridge is quite high.
The time to cool a block from room temperature to suitable for sectioning is only about two minutes.
Now, because Norway has a relatively cold climate, the air humidity is also quite low compared to warmer climates, even if it rains here ALL the time... ;-)
Heat and high humidity might affect your blocks.

Gudrun H. Erikstad, Trondheim, Norway



-----Opprinnelig melding-----
Fra: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] P? vegne av Itai Moshe
Sendt: 20. september 2011 08:23
Til: histonet@lists.utsouthwestern.edu
Emne: [Histonet] How to store paraffin blocks ?

Hi Histonet's,

What is the recommended way to store paraffin blocks for a long period ?
Room temperature, or 4C ?

The advantage in storing at 4C is that the blocks are always ready for sectioning.
Is there a risk when storing at 4C that the humidity in the fridge will cause damage to the paraffin blocks and the tissue ?
After sectioning, How do you store the slides, and for how long ?


Thank's
Itai Moshe
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From thisisann <@t> aol.com  Tue Sep 20 09:09:41 2011
From: thisisann <@t> aol.com (Ann Angelo)
Date: Tue Sep 20 09:09:51 2011
Subject: [Histonet] Verification System
Message-ID: <8CE45B6132A22E2-7D8-725E9@webmail-d167.sysops.aol.com>


I am looking for a verification system which does not require the data entry to be performed prior to cutting (ruling out Vantage and Cerebro).  I have looked at Thermo's slidemate but can't get them to move fast enough.
Does anyone know of another verification system that I may be able to try which does not require the data entry to be performed prior to microtomy?  Thanks, Ann



From rjbuesa <@t> yahoo.com  Tue Sep 20 09:19:47 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Sep 20 09:19:54 2011
Subject: [Histonet] How to store paraffin blocks ?
In-Reply-To: 
Message-ID: <1316528387.5562.YahooMailClassic@web65706.mail.ac4.yahoo.com>

The usual procedure is to store at room temperature. It is assumed that the room is air conditioned, otherwise if the temp is above 80?F some blocks may stick together, although that is not a real problem.
If at 4?C you will need a refrigerated room or a series of refrigerators, which is an additional and unnecessary expenditure.
We use to store the blocks for 9 years, unless it is a special case (used in teaching) and those are stored forever.
Supposedly you use the slides for some procedure and those cut as "extras" are kept along with the originals stained in the same files.
If you are referring to sections for IHC or other procedure, the epitopes will be oxidized by the oxygen in the air in less than 2 weeks, so they can either be stored in a fridge, or covered with melted paraffin, or stored in mineral oil.
Ren? J.

--- On Tue, 9/20/11, Itai Moshe  wrote:


From: Itai Moshe 
Subject: [Histonet] How to store paraffin blocks ?
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 20, 2011, 2:23 AM


Hi Histonet's,

What is the recommended way to store paraffin blocks for a long period ?
Room temperature, or 4C ?

The advantage in storing at 4C is that the blocks are always ready for
sectioning.
Is there a risk when storing at 4C that the humidity in the fridge will
cause damage to the paraffin blocks and the tissue ?
After sectioning, How do you store the slides, and for how long ?


Thank's
Itai Moshe
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From MSHERWOOD <@t> PARTNERS.ORG  Tue Sep 20 09:58:52 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret)
Date: Tue Sep 20 09:59:01 2011
Subject: [Histonet] How to store paraffin blocks ?
In-Reply-To: <1316528387.5562.YahooMailClassic@web65706.mail.ac4.yahoo.com>
References: 
	<1316528387.5562.YahooMailClassic@web65706.mail.ac4.yahoo.com>
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A01@PHSXMB30.partners.org>

We are a research group, but had paraffin blocks stored at room temperature for
20+ years.  We finally made the decision to just keep 2 years in the lab and
store 5 years off-site.  We trashed the rest.

Peggy 


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, September 20, 2011 10:20 AM
To: histonet@lists.utsouthwestern.edu; Itai Moshe
Subject: Re: [Histonet] How to store paraffin blocks ?

The usual procedure is to store at room temperature. It is assumed that the room
is air conditioned, otherwise if the temp is above 80?F some blocks may stick
together, although that is not a real problem.
If at 4?C you will need a refrigerated room or a series of refrigerators, which
is an additional and unnecessary expenditure.
We use to store the blocks for 9 years, unless it is a special case (used in
teaching) and those are stored forever.
Supposedly you use the slides for some procedure and those cut as "extras" are
kept along with the originals stained in the same files.
If you are referring to sections for IHC or other procedure, the epitopes will
be oxidized by the oxygen in the air in less than 2 weeks, so they can either be
stored in a fridge, or covered with melted paraffin, or stored in mineral oil.
Ren? J.

--- On Tue, 9/20/11, Itai Moshe  wrote:


From: Itai Moshe 
Subject: [Histonet] How to store paraffin blocks ?
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 20, 2011, 2:23 AM


Hi Histonet's,

What is the recommended way to store paraffin blocks for a long period ?
Room temperature, or 4C ?

The advantage in storing at 4C is that the blocks are always ready for
sectioning.
Is there a risk when storing at 4C that the humidity in the fridge will
cause damage to the paraffin blocks and the tissue ?
After sectioning, How do you store the slides, and for how long ?


Thank's
Itai Moshe
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


From Timothy.Morken <@t> ucsfmedctr.org  Tue Sep 20 11:44:26 2011
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy)
Date: Tue Sep 20 11:44:34 2011
Subject: [Histonet] Tissue handling at embedding
Message-ID: <8D7C2D242DBD45498006B21122072BF85E2AEE21@MCINFRWEM003.ucsfmedicalcenter.org>

What procedures do you have in place to prevent tissue loss at embedding? What do you do if tissue appears to have been lost?

And do you clean embedding molds before each reuse, or after one-days use (which may be many re-uses)?


Thanks for all info!


Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA

415.514.6042 (office)
415.885.7409 Fax
tim.morken@ucsfmedctr.org


From amario3 <@t> uic.edu  Tue Sep 20 11:59:19 2011
From: amario3 <@t> uic.edu (Andrea Marion)
Date: Tue Sep 20 11:59:22 2011
Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence
Message-ID: <6623612296b9078e4db4eccef8f88c74.squirrel@webmail.uic.edu>

Hello all,

Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported to
be a universal antigen retrieval solution that can be used at lower
temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear
antigens):

http://innvx.com/unitrievepage.html

The company claims that the reagent is a universal retrieval solution for
all antibodies and tissues (which is silly of course - how could they
know?). Does anyone have any experience with the product?

I am interested because I see that increased heat during antigen retrieval
causes greater tissue autofluorescence during immunofluorescence
stainings. My current protocol is to use 20 minutes at 90-95 C on a hot
plate using sodium citrate buffer. Does anyone else either Uni-trieve or a
different reagent/protocol for immunofluorescence stainings?

Thanks,

Andrea

Andrea Marion
Graduate Student
University of Illinois at Chicago


From thisisann <@t> aol.com  Tue Sep 20 12:20:14 2011
From: thisisann <@t> aol.com (Ann Angelo)
Date: Tue Sep 20 12:20:20 2011
Subject: [Histonet] Legal Case SOP
Message-ID: <8CE45D0B1BF937B-2198-21BC4@webmail-d008.sysops.aol.com>

Can anyone share their SOP regarding the handling of legal cases (I.e. if a specimen is being requested from an outside source which is not the patient).  Thank you, Ann


From sdysart <@t> mirnarx.com  Tue Sep 20 12:49:06 2011
From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com)
Date: Tue Sep 20 12:49:10 2011
Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence
In-Reply-To: <6623612296b9078e4db4eccef8f88c74.squirrel@webmail.uic.edu>
References: <6623612296b9078e4db4eccef8f88c74.squirrel@webmail.uic.edu>
Message-ID: 

I actually attended a class they put on a few months ago where they used
Unitrieve.  It works really well.  It doesn't seem to produce any kind
of background and yes, it is used at much lower temps.  However the
retrieval process takes a little longer, but...you can put it in an even
lower temp. water bath overnight, come in to work in the morning, and
your slides are reading to go.
Contact Zara over there and she might be able to hook you up with a
sample.
Good Luck!!

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea
Marion
Sent: Tuesday, September 20, 2011 11:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence

Hello all,

Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported
to
be a universal antigen retrieval solution that can be used at lower
temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear
antigens):

http://innvx.com/unitrievepage.html

The company claims that the reagent is a universal retrieval solution
for
all antibodies and tissues (which is silly of course - how could they
know?). Does anyone have any experience with the product?

I am interested because I see that increased heat during antigen
retrieval
causes greater tissue autofluorescence during immunofluorescence
stainings. My current protocol is to use 20 minutes at 90-95 C on a hot
plate using sodium citrate buffer. Does anyone else either Uni-trieve or
a
different reagent/protocol for immunofluorescence stainings?

Thanks,

Andrea

Andrea Marion
Graduate Student
University of Illinois at Chicago


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From JMacDonald <@t> mtsac.edu  Tue Sep 20 13:23:55 2011
From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald)
Date: Tue Sep 20 13:23:59 2011
Subject: [Histonet] cost of an H&E
Message-ID: 

Has anyone calculated the approximate cost of an H&E, materials only, no 
labor?  Including the cost of the cassette, paraffin, slide, hematoxylin, 
eosin and staining reagents?
Thanks,
Jennifer MacDonald
Mt. San Antonio College
From Lynn.Burton <@t> Illinois.gov  Tue Sep 20 13:30:12 2011
From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn)
Date: Tue Sep 20 13:30:55 2011
Subject: [Histonet] cost of an H&E
In-Reply-To: 
References: 
Message-ID: <4A6E2CACA1E017408EBA1B9911952CC00643DD12DF@IL084EXMBX214.illinois.gov>

We calculated that to be right around $4.05 per slide. That does not include labor.

Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald [JMacDonald@mtsac.edu]
Sent: Tuesday, September 20, 2011 1:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cost of an H&E

Has anyone calculated the approximate cost of an H&E, materials only, no
labor?  Including the cost of the cassette, paraffin, slide, hematoxylin,
eosin and staining reagents?
Thanks,
Jennifer MacDonald
Mt. San Antonio College
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From andrewcoleman131 <@t> gmail.com  Tue Sep 20 14:50:51 2011
From: andrewcoleman131 <@t> gmail.com (Andrew Coleman)
Date: Tue Sep 20 14:51:04 2011
Subject: [Histonet] Effect of Freezing on Unfixed GFP
Message-ID: 

We have performed lentiviral injections with a turboGreenFluorescentProtein
(tGFP) into the brains of rats for an RNAi experiment. We did not fix the
tissue but froze in isopentane on dry ice and stored the tissue at -80 deg
Celsius.

We want to use some sections for a reference of the injection site (GFP
fluorescence) relative to the brain morphology (Neurotracer staining) in
order to selecitvely laser microdissect (LMD) infected cells from other
sections.

Our initial studies showed that fixation of sections on slides in 4%
paraformaledhyde (PFA) at 4 deg C for 5 minutes provided tGFP flourescence
comparable to that found in perfused tissue, but lately we see very weak or
no fluorescence with this procedure, even when increasing the time.

The only difference I can think of that we saw best results soon after the
brains were removed (ie 1 to 2 weeks) and now we are having trouble
visualizing the tGFP ~2 months of the brains being stored at -80 degrees
celsius.

Does anyone have experience with or an explanation of fluorescence
decreasing with a frozen, unfixed GFP over time?

In the future we plan on doing these experiments including a perfusion with
a low conc of PFA (to preserve RNA integrity) but for now we are trying to
figure out how to identify the GFP-positive cells in the animals we have
already performed surgery on. Another option would anti-tGFP IHC, but we
would prefer at this point to use on slide-fixed sections as a references
for our LMD.

Thanks!  -Andrew
From rjbuesa <@t> yahoo.com  Tue Sep 20 14:51:45 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Sep 20 14:51:48 2011
Subject: [Histonet] Tissue handling at embedding
In-Reply-To: <8D7C2D242DBD45498006B21122072BF85E2AEE21@MCINFRWEM003.ucsfmedicalcenter.org>
Message-ID: <1316548305.7251.YahooMailClassic@web65716.mail.ac4.yahoo.com>

This is how we do it:
1- the person grossing will dictate how many pieces should go into each cassette
2- the person cassetting will check that information against the vial with the pieces and write down in the cassettes summary form the number of pieces
3- the person embedding checks the pieces in the cassette after processing against the cassettes log.
If any piece is missing between any two steps, it will be looked for. Sometimes is small and is in the vial before cassetting, or small and in the retort.
Any irregularity is documented in the QA log.
Ren? J.

--- On Tue, 9/20/11, Morken, Timothy  wrote:


From: Morken, Timothy 
Subject: [Histonet] Tissue handling at embedding
To: "histonet@lists.utsouthwestern.edu" 
Date: Tuesday, September 20, 2011, 12:44 PM


What procedures do you have in place to prevent tissue loss at embedding? What do you do if tissue appears to have been lost?

And do you clean embedding molds before each reuse, or after one-days use (which may be many re-uses)?


Thanks for all info!


Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA

415.514.6042 (office)
415.885.7409 Fax
tim.morken@ucsfmedctr.org


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From rjbuesa <@t> yahoo.com  Tue Sep 20 14:53:12 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Sep 20 14:53:15 2011
Subject: [Histonet] cost of an H&E
In-Reply-To: 
Message-ID: <1316548392.93704.YahooMailClassic@web65710.mail.ac4.yahoo.com>

Under separate cover I am sending something I wrote about this subject.
Ren? J.

--- On Tue, 9/20/11, Jennifer MacDonald  wrote:


From: Jennifer MacDonald 
Subject: [Histonet] cost of an H&E
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 20, 2011, 2:23 PM


Has anyone calculated the approximate cost of an H&E, materials only, no 
labor?? Including the cost of the cassette, paraffin, slide, hematoxylin, 
eosin and staining reagents?
Thanks,
Jennifer MacDonald
Mt. San Antonio College
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From thiggins <@t> cddmedical.com  Tue Sep 20 15:03:06 2011
From: thiggins <@t> cddmedical.com (Tim Higgins)
Date: Tue Sep 20 15:03:11 2011
Subject: [Histonet] Tissue handling at embedding
References: <20110920170504.431A5142450B@barracuda.crvinc.net>
Message-ID: <001101cc77d0$50b7afa0$e001a8c0@cdd.loc>

I have worked at labs that reuse molds and ones that clean between uses.
Guess it all depends on what your definition of a "clean" mold is.  If you
have the time and space, not reusing the molds is best practice but not
always practical.

Thanks,

Tim


Message: 10
Date: Tue, 20 Sep 2011 09:44:26 -0700
From: "Morken, Timothy" 
Subject: [Histonet] Tissue handling at embedding
To: "histonet@lists.utsouthwestern.edu"

Message-ID:
<8D7C2D242DBD45498006B21122072BF85E2AEE21@MCINFRWEM003.ucsfmedicalcenter.org
>

Content-Type: text/plain; charset=us-ascii

What procedures do you have in place to prevent tissue loss at embedding?
What do you do if tissue appears to have been lost?

And do you clean embedding molds before each reuse, or after one-days use
(which may be many re-uses)?


Thanks for all info!


Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA

415.514.6042 (office)
415.885.7409 Fax
tim.morken@ucsfmedctr.org




From amario3 <@t> uic.edu  Tue Sep 20 15:19:48 2011
From: amario3 <@t> uic.edu (Andrea Marion)
Date: Tue Sep 20 15:19:51 2011
Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence
In-Reply-To: 
References: <6623612296b9078e4db4eccef8f88c74.squirrel@webmail.uic.edu>
	
Message-ID: 

Hi Sarah,

Thanks for the info! Have you used Unitrieve for immunofluorescence? I am
wondering whether I will see a reduction in the autofluorescence caused by
heating? I will request a sample to try and report back to all.

Andrea

Andrea Marion
Graduate Student
University of Illinois at Chicago

On Tue, September 20, 2011 12:49 pm, sdysart@mirnarx.com wrote:
> I actually attended a class they put on a few months ago where they used
> Unitrieve.  It works really well.  It doesn't seem to produce any kind
> of background and yes, it is used at much lower temps.  However the
> retrieval process takes a little longer, but...you can put it in an even
> lower temp. water bath overnight, come in to work in the morning, and
> your slides are reading to go.
> Contact Zara over there and she might be able to hook you up with a
> sample.
> Good Luck!!
>
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas  78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea
> Marion
> Sent: Tuesday, September 20, 2011 11:59 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence
>
> Hello all,
>
> Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported
> to
> be a universal antigen retrieval solution that can be used at lower
> temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear
> antigens):
>
> http://innvx.com/unitrievepage.html
>
> The company claims that the reagent is a universal retrieval solution
> for
> all antibodies and tissues (which is silly of course - how could they
> know?). Does anyone have any experience with the product?
>
> I am interested because I see that increased heat during antigen
> retrieval
> causes greater tissue autofluorescence during immunofluorescence
> stainings. My current protocol is to use 20 minutes at 90-95 C on a hot
> plate using sodium citrate buffer. Does anyone else either Uni-trieve or
> a
> different reagent/protocol for immunofluorescence stainings?
>
> Thanks,
>
> Andrea
>
> Andrea Marion
> Graduate Student
> University of Illinois at Chicago
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>



From cebass <@t> buffalo.edu  Tue Sep 20 16:30:26 2011
From: cebass <@t> buffalo.edu (Caroline Bass)
Date: Tue Sep 20 16:30:30 2011
Subject: [Histonet] used microscope vendors
Message-ID: <49365679-58DD-48DE-80D5-0355BBA95515@buffalo.edu>

Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out. 

Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units.

Thanks,

Caroline
From Lacie.Algeo <@t> providence.org  Tue Sep 20 18:21:05 2011
From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A)
Date: Tue Sep 20 18:21:10 2011
Subject: [Histonet] C4d 
Message-ID: <6A73DCDB9E46D2489D4001B4001C92AE012C4349@IRMEXCH05.irm.inhs.org>

Hi Everyone,
Does anyone know of a FITC C4d that is ASR or IVD?  All I have been able
to find is RUO.  Also, is anyone using something different to show
rejection in transplant kidneys?
Thank you,
Lacie

Lacie Algeo, HTL, MP(ASCP)
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
Spokane, WA 99220
509-474-4418
FAX 509-474-2052
lacie.algeo@providence.org

From Elizabeth.Dickert <@t> hcahealthcare.com  Wed Sep 21 05:45:14 2011
From: Elizabeth.Dickert <@t> hcahealthcare.com (Elizabeth.Dickert@hcahealthcare.com)
Date: Wed Sep 21 05:45:19 2011
Subject: [Histonet] FW: Procedure for SOS11
Message-ID: <0226177269DFDE48A23F40D00634258B15EF6C83EE@NADCWPMSGCMS10.hca.corpad.net>



Elizabeth Ann Dickert
Southern Pathology
2333 McCallie Ave.
Chattanooga, TN.
423-493-6904

_____________________________________________
From: Dickert Elizabeth
Sent: Thursday, September 15, 2011 10:35 AM
To: 'histonet-request@lists.utsouthwestern.edu'
Subject: SOX11


Anyone doing a sox11 with good results?
Have Abcam antibody / Ventana equipment (ultraview detection)

Elizabeth Ann Dickert
Southern Pathology
2333 McCallie Ave.
Chattanooga, TN.
423-493-6904
elizabeth.dickert@hcahealthcare.com

From Margaret.Perry <@t> sdstate.edu  Wed Sep 21 07:55:47 2011
From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret)
Date: Wed Sep 21 07:55:52 2011
Subject: [Histonet] unstructured proteins
Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE012722@SDSU-EX03.jacks.local>

Have any of you worked with the unstructured proteins?  Do they form the same crosslinks when fixed with formalin?  Would antigen retrieval be of any use?

Margaret Perry HT(ASCP)
Dept of Veterinary and  Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638

From trathborne <@t> somerset-healthcare.com  Wed Sep 21 08:54:00 2011
From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni)
Date: Wed Sep 21 08:54:25 2011
Subject: [Histonet] used microscope vendors
In-Reply-To: <49365679-58DD-48DE-80D5-0355BBA95515@buffalo.edu>
References: <49365679-58DD-48DE-80D5-0355BBA95515@buffalo.edu>
Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F56F73@SMCMAIL01.somerset-healthcare.com>

What part of the country are you in?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Bass
Sent: Tuesday, September 20, 2011 5:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] used microscope vendors

Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out. 

Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units.

Thanks,

Caroline
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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From sbaldwin <@t> mhhcc.org  Wed Sep 21 09:10:59 2011
From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org)
Date: Wed Sep 21 09:11:08 2011
Subject: [Histonet] Napsin A controls
Message-ID: 

Hi Histonetters
Are there any other controls for Napsin A other than Lung adenocarcinoma?

Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-996-0210, 0216,  Fax 812-996-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
From rjbuesa <@t> yahoo.com  Wed Sep 21 09:12:40 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Sep 21 09:12:48 2011
Subject: [Histonet] used microscope vendors
In-Reply-To: <49365679-58DD-48DE-80D5-0355BBA95515@buffalo.edu>
Message-ID: <1316614360.28378.YahooMailClassic@web65703.mail.ac4.yahoo.com>

Try eBay. I have seen good instruments for sale there.
Ren? J.

--- On Tue, 9/20/11, Caroline Bass  wrote:


From: Caroline Bass 
Subject: [Histonet] used microscope vendors
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 20, 2011, 5:30 PM


Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out. 

Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units.

Thanks,

Caroline
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From mpence <@t> grhs.net  Wed Sep 21 09:15:32 2011
From: mpence <@t> grhs.net (Mike Pence)
Date: Wed Sep 21 09:15:38 2011
Subject: [Histonet] Napsin A controls
In-Reply-To: 
Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974CBD@is-e2k3.grhs.net>

Kidney

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara
Baldwin/mhhcc.org
Sent: Wednesday, September 21, 2011 9:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Napsin A controls


Hi Histonetters
Are there any other controls for Napsin A other than Lung
adenocarcinoma?

Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-996-0210, 0216,  Fax 812-996-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From tnguyen <@t> kaiserassociates.com  Wed Sep 21 10:22:15 2011
From: tnguyen <@t> kaiserassociates.com (Tieuvi Nguyen)
Date: Wed Sep 21 10:24:44 2011
Subject: [Histonet] Paid Survey ($50) Opportunity - Digital Pathology
Message-ID: <61C52EFB4F0AF8498D9ECADA6796B46620E26EDE@VA3DIAXVS3D1.RED001.local>

Hi Everyone,

I had sent a note out to the group about a month ago inviting participants to take part in phone interviews to discuss Digital Pathology technology.  We are now following up those interviews with an online survey on the same subject.  We are particularly interested in individuals that work in high volume commercial or reference labs (i.e. process more than 100,000 slides a year, and process more than 5,000 IHC slides a year).  You do not have to be a current user of digital pathology to participate.  The survey will begin with a few questions to determine if you qualify for the survey.  If you qualify and complete the survey you will receive an honoraria in the form of a $50 American Express gift card for your participation.  All answers will remain anonymous.

The survey should take about 15-20 minutes to complete.

You can access the survey here:

http://www.keysurvey.com/survey/387885/297f/

Please feel free to contact me if you have any questions.


Tieuvi Nguyen, PhD  /  Kaiser Associates, Inc.
M +1 202 506 0775  /  tnguyen@kaiserassociates.com
1201 Connecticut Ave NW, 12th Floor  /  Washington, DC  20036  USA
www.kaiserassociates.com
From sbreeden <@t> nmda.nmsu.edu  Wed Sep 21 11:07:31 2011
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Wed Sep 21 11:07:44 2011
Subject: [Histonet] Klotz Solution
Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFACC@nmdamailsvr.nmda.ad.nmsu.edu>

I've just "googled" and "histosearched" for Klotz solution but cannot
find anything that doesn't require chloral hydrate.  Has anyone made a
Big Breakthrough in making Klotz that can be made without that pesky
controlled substance?  Any help will be appreciated...who knew the
recipe joined the Witness Protection Program!

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

From Karen.Heckford <@t> CHW.edu  Wed Sep 21 11:29:07 2011
From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF)
Date: Wed Sep 21 11:30:05 2011
Subject: [Histonet] Urgent!! Need per diem
Message-ID: <9ECF174E7DA83046BE6EBFDE009E28A388C456@CHW-MSG-301.chw.edu>

I am looking for a Certified ASCP HistoTech. that is local to the San
Francisco Bay Area.  I need this person to cover me while I am out
having knee surgery.  I will be out 2-6 weeks probably more like 4 weeks
starting October 5th.  But need somebody by October 3rd to do some
training.  

 

You will need to know IHC and Special stains and be able to work
independently because I am the Chief, Cook and Bottle washer here.  

 

Thanks,

 

Karen Heckford HT ASCP CE

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-668-1000 ext. 6167Caution:  This email message, including all
content and attachments, is CONFIDENTIAL and may be of a nature that is
LEGALLY PRIVILEGED.  The information contained in this email message is
intended only for the use of the recipient(s) named above. If the reader
of this message is not the intended recipient or an agent responsible
for delivering it to the intended recipient, you have received this
document in error.  Any further review, dissemination, distribution, or
copying of this message is strictly prohibited.  If you have received
this communication in error, please notify us  immediately by reply
email.  Thank you."



 

 

From Sandra.Harrison3 <@t> va.gov  Wed Sep 21 12:17:01 2011
From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.)
Date: Wed Sep 21 12:17:09 2011
Subject: [Histonet] Leica cassette and slide printer ink
Message-ID: 

I have been told that I can no longer buy only the replacement ink
cartridges for Leica cassette and slide printers.  The ink cartridge was
already an outrageous $411.00 each.  Now you have to by a kit for
$566.00 each.  The kit includes a replacement plate along with the ink.

  

Why in the world the replacement plate would need to be replaced every
time you replace an expired ink cartridge is beyond me, other than a
shameless scam to up the price on the already completely overpriced ink
cartridge.

 

Has everybody been told this by their Leica distributor?  If so, I think
a lot of us will be looking HARD at other instruments when it comes time
to replace slide/cassette printers!

 

Sandy C. Harrison, HTL (ASCP)

Histology Supervisor

Minneapolis VA

612-467-2449

 

From jshelley <@t> sanfordburnham.org  Wed Sep 21 12:28:29 2011
From: jshelley <@t> sanfordburnham.org (John Shelley)
Date: Wed Sep 21 12:28:38 2011
Subject: [Histonet] RE: Klotz Solution
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DFACC@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DFACC@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <5A605CE38EECB64B94485C02125A0C440716203E@LN-MAIL07.ln.burnham.org>

http://www.histosearch.com/histonet/Sep99/RE.FormulaforlongtermquotA.html

I think this is what you are looking for. Have a great day!!!


Kind Regards!
?
John J Shelley
Senior Research Associate, Histology Core Facility

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Wednesday, September 21, 2011 12:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Klotz Solution

I've just "googled" and "histosearched" for Klotz solution but cannot
find anything that doesn't require chloral hydrate.  Has anyone made a
Big Breakthrough in making Klotz that can be made without that pesky
controlled substance?  Any help will be appreciated...who knew the
recipe joined the Witness Protection Program!

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Sheri.Meilus <@t> va.gov  Wed Sep 21 12:38:09 2011
From: Sheri.Meilus <@t> va.gov (Meilus, Sheri D.)
Date: Wed Sep 21 12:38:22 2011
Subject: [Histonet] RE: Napsin A Controls
In-Reply-To: <9e149238-e73b-4d46-8118-0bd21a3ae550@VAPNSMSGH2.vha.med.va.gov>
References: <9e149238-e73b-4d46-8118-0bd21a3ae550@VAPNSMSGH2.vha.med.va.gov>
Message-ID: <05412F1B056CCB47A6CB76FB4EF67F84013AFFA0@VHAV08MSGE5.v08.med.va.gov>

Normal lung also contains good internal positive controls such as Type II pneumocytes, histiocytes and macrophages.
Best regards,

S
Sheri Meilus
Anatomic Pathology Supervisor
Bay Pines VAHC
Building 100
Room 2B-126
727-398-6661 ext 4596



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Wednesday, September 21, 2011 1:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 94, Issue 24

Send Histonet mailing list submissions to
	histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Legal Case SOP (Ann Angelo)
   2. RE: Uni-trieve, antigen retrieval for immunofluorescence
      (sdysart@mirnarx.com)
   3. cost of an H&E (Jennifer MacDonald)
   4. RE: cost of an H&E (Burton, Lynn)
   5. Effect of Freezing on Unfixed GFP (Andrew Coleman)
   6. Re: Tissue handling at embedding (Rene J Buesa)
   7. Re: cost of an H&E (Rene J Buesa)
   8. Tissue handling at embedding (Tim Higgins)
   9. RE: Uni-trieve, antigen retrieval for immunofluorescence
      (Andrea Marion)
  10. used microscope vendors (Caroline Bass)
  11. C4d  (Algeo, Lacie A)
  12. FW: Procedure for SOS11 (Elizabeth.Dickert@hcahealthcare.com)
  13. unstructured proteins (Perry, Margaret)
  14. RE: used microscope vendors (Rathborne, Toni)
  15. Napsin A controls (Sara Baldwin/mhhcc.org)
  16. Re: used microscope vendors (Rene J Buesa)
  17. RE: Napsin A controls (Mike Pence)
  18. Paid Survey ($50) Opportunity - Digital Pathology (Tieuvi Nguyen)
  19. Klotz Solution (Breeden, Sara)
  20. Urgent!! Need per diem (Heckford, Karen - SMMC-SF)


----------------------------------------------------------------------

Message: 1
Date: Tue, 20 Sep 2011 13:20:14 -0400 (EDT)
From: Ann Angelo 
Subject: [Histonet] Legal Case SOP
To: histonet@lists.utsouthwestern.edu
Message-ID: <8CE45D0B1BF937B-2198-21BC4@webmail-d008.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

Can anyone share their SOP regarding the handling of legal cases (I.e. if a specimen is being requested from an outside source which is not the patient).  Thank you, Ann




------------------------------

Message: 2
Date: Tue, 20 Sep 2011 12:49:06 -0500
From: 
Subject: RE: [Histonet] Uni-trieve, antigen retrieval for
	immunofluorescence
To: ,	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

I actually attended a class they put on a few months ago where they used
Unitrieve.  It works really well.  It doesn't seem to produce any kind
of background and yes, it is used at much lower temps.  However the
retrieval process takes a little longer, but...you can put it in an even
lower temp. water bath overnight, come in to work in the morning, and
your slides are reading to go.
Contact Zara over there and she might be able to hook you up with a
sample.
Good Luck!!

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea
Marion
Sent: Tuesday, September 20, 2011 11:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence

Hello all,

Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported
to
be a universal antigen retrieval solution that can be used at lower
temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear
antigens):

http://innvx.com/unitrievepage.html

The company claims that the reagent is a universal retrieval solution
for
all antibodies and tissues (which is silly of course - how could they
know?). Does anyone have any experience with the product?

I am interested because I see that increased heat during antigen
retrieval
causes greater tissue autofluorescence during immunofluorescence
stainings. My current protocol is to use 20 minutes at 90-95 C on a hot
plate using sodium citrate buffer. Does anyone else either Uni-trieve or
a
different reagent/protocol for immunofluorescence stainings?

Thanks,

Andrea

Andrea Marion
Graduate Student
University of Illinois at Chicago


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 3
Date: Tue, 20 Sep 2011 11:23:55 -0700
From: Jennifer MacDonald 
Subject: [Histonet] cost of an H&E
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset="US-ASCII"

Has anyone calculated the approximate cost of an H&E, materials only, no 
labor?  Including the cost of the cassette, paraffin, slide, hematoxylin, 
eosin and staining reagents?
Thanks,
Jennifer MacDonald
Mt. San Antonio College

------------------------------

Message: 4
Date: Tue, 20 Sep 2011 13:30:12 -0500
From: "Burton, Lynn" 
Subject: RE: [Histonet] cost of an H&E
To: Jennifer MacDonald ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<4A6E2CACA1E017408EBA1B9911952CC00643DD12DF@IL084EXMBX214.illinois.gov>
	
Content-Type: text/plain; charset="us-ascii"

We calculated that to be right around $4.05 per slide. That does not include labor.

Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald [JMacDonald@mtsac.edu]
Sent: Tuesday, September 20, 2011 1:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cost of an H&E

Has anyone calculated the approximate cost of an H&E, materials only, no
labor?  Including the cost of the cassette, paraffin, slide, hematoxylin,
eosin and staining reagents?
Thanks,
Jennifer MacDonald
Mt. San Antonio College
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 5
Date: Tue, 20 Sep 2011 15:50:51 -0400
From: Andrew Coleman 
Subject: [Histonet] Effect of Freezing on Unfixed GFP
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

We have performed lentiviral injections with a turboGreenFluorescentProtein
(tGFP) into the brains of rats for an RNAi experiment. We did not fix the
tissue but froze in isopentane on dry ice and stored the tissue at -80 deg
Celsius.

We want to use some sections for a reference of the injection site (GFP
fluorescence) relative to the brain morphology (Neurotracer staining) in
order to selecitvely laser microdissect (LMD) infected cells from other
sections.

Our initial studies showed that fixation of sections on slides in 4%
paraformaledhyde (PFA) at 4 deg C for 5 minutes provided tGFP flourescence
comparable to that found in perfused tissue, but lately we see very weak or
no fluorescence with this procedure, even when increasing the time.

The only difference I can think of that we saw best results soon after the
brains were removed (ie 1 to 2 weeks) and now we are having trouble
visualizing the tGFP ~2 months of the brains being stored at -80 degrees
celsius.

Does anyone have experience with or an explanation of fluorescence
decreasing with a frozen, unfixed GFP over time?

In the future we plan on doing these experiments including a perfusion with
a low conc of PFA (to preserve RNA integrity) but for now we are trying to
figure out how to identify the GFP-positive cells in the animals we have
already performed surgery on. Another option would anti-tGFP IHC, but we
would prefer at this point to use on slide-fixed sections as a references
for our LMD.

Thanks!  -Andrew


------------------------------

Message: 6
Date: Tue, 20 Sep 2011 12:51:45 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] Tissue handling at embedding
To: "histonet@lists.utsouthwestern.edu"
	,	TimothyMorken
	
Message-ID:
	<1316548305.7251.YahooMailClassic@web65716.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

This is how we do it:
1- the person grossing will dictate how many pieces should go into each cassette
2- the person cassetting will check that information against the vial with the pieces and write down in the cassettes summary form the number of pieces
3- the person embedding checks the pieces in the cassette after processing against the cassettes log.
If any piece is missing between any two steps, it will be looked for. Sometimes is small and is in the vial before cassetting, or small and in the retort.
Any irregularity is documented in the QA log.
Ren? J.

--- On Tue, 9/20/11, Morken, Timothy  wrote:


From: Morken, Timothy 
Subject: [Histonet] Tissue handling at embedding
To: "histonet@lists.utsouthwestern.edu" 
Date: Tuesday, September 20, 2011, 12:44 PM


What procedures do you have in place to prevent tissue loss at embedding? What do you do if tissue appears to have been lost?

And do you clean embedding molds before each reuse, or after one-days use (which may be many re-uses)?


Thanks for all info!


Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA

415.514.6042 (office)
415.885.7409 Fax
tim.morken@ucsfmedctr.org


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 7
Date: Tue, 20 Sep 2011 12:53:12 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] cost of an H&E
To: histonet@lists.utsouthwestern.edu,	Jennifer MacDonald
	
Message-ID:
	<1316548392.93704.YahooMailClassic@web65710.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Under separate cover I am sending something I wrote about this subject.
Ren? J.

--- On Tue, 9/20/11, Jennifer MacDonald  wrote:


From: Jennifer MacDonald 
Subject: [Histonet] cost of an H&E
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 20, 2011, 2:23 PM


Has anyone calculated the approximate cost of an H&E, materials only, no 
labor?? Including the cost of the cassette, paraffin, slide, hematoxylin, 
eosin and staining reagents?
Thanks,
Jennifer MacDonald
Mt. San Antonio College
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 8
Date: Tue, 20 Sep 2011 15:03:06 -0500
From: "Tim Higgins" 
Subject: [Histonet] Tissue handling at embedding
To: 
Message-ID: <001101cc77d0$50b7afa0$e001a8c0@cdd.loc>
Content-Type: text/plain;	charset="iso-8859-1"

I have worked at labs that reuse molds and ones that clean between uses.
Guess it all depends on what your definition of a "clean" mold is.  If you
have the time and space, not reusing the molds is best practice but not
always practical.

Thanks,

Tim


Message: 10
Date: Tue, 20 Sep 2011 09:44:26 -0700
From: "Morken, Timothy" 
Subject: [Histonet] Tissue handling at embedding
To: "histonet@lists.utsouthwestern.edu"

Message-ID:
<8D7C2D242DBD45498006B21122072BF85E2AEE21@MCINFRWEM003.ucsfmedicalcenter.org
>

Content-Type: text/plain; charset=us-ascii

What procedures do you have in place to prevent tissue loss at embedding?
What do you do if tissue appears to have been lost?

And do you clean embedding molds before each reuse, or after one-days use
(which may be many re-uses)?


Thanks for all info!


Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA

415.514.6042 (office)
415.885.7409 Fax
tim.morken@ucsfmedctr.org






------------------------------

Message: 9
Date: Tue, 20 Sep 2011 15:19:48 -0500
From: "Andrea Marion" 
Subject: RE: [Histonet] Uni-trieve, antigen retrieval for
	immunofluorescence
To: sdysart@mirnarx.com
Cc: histonet@lists.utsouthwestern.edu, amario3@uic.edu
Message-ID:
	
Content-Type: text/plain;charset=iso-8859-1

Hi Sarah,

Thanks for the info! Have you used Unitrieve for immunofluorescence? I am
wondering whether I will see a reduction in the autofluorescence caused by
heating? I will request a sample to try and report back to all.

Andrea

Andrea Marion
Graduate Student
University of Illinois at Chicago

On Tue, September 20, 2011 12:49 pm, sdysart@mirnarx.com wrote:
> I actually attended a class they put on a few months ago where they used
> Unitrieve.  It works really well.  It doesn't seem to produce any kind
> of background and yes, it is used at much lower temps.  However the
> retrieval process takes a little longer, but...you can put it in an even
> lower temp. water bath overnight, come in to work in the morning, and
> your slides are reading to go.
> Contact Zara over there and she might be able to hook you up with a
> sample.
> Good Luck!!
>
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas  78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea
> Marion
> Sent: Tuesday, September 20, 2011 11:59 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence
>
> Hello all,
>
> Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported
> to
> be a universal antigen retrieval solution that can be used at lower
> temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear
> antigens):
>
> http://innvx.com/unitrievepage.html
>
> The company claims that the reagent is a universal retrieval solution
> for
> all antibodies and tissues (which is silly of course - how could they
> know?). Does anyone have any experience with the product?
>
> I am interested because I see that increased heat during antigen
> retrieval
> causes greater tissue autofluorescence during immunofluorescence
> stainings. My current protocol is to use 20 minutes at 90-95 C on a hot
> plate using sodium citrate buffer. Does anyone else either Uni-trieve or
> a
> different reagent/protocol for immunofluorescence stainings?
>
> Thanks,
>
> Andrea
>
> Andrea Marion
> Graduate Student
> University of Illinois at Chicago
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>





------------------------------

Message: 10
Date: Tue, 20 Sep 2011 17:30:26 -0400
From: Caroline Bass 
Subject: [Histonet] used microscope vendors
To: histonet@lists.utsouthwestern.edu
Message-ID: <49365679-58DD-48DE-80D5-0355BBA95515@buffalo.edu>
Content-Type: text/plain; charset=us-ascii

Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out. 

Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units.

Thanks,

Caroline


------------------------------

Message: 11
Date: Tue, 20 Sep 2011 16:21:05 -0700
From: "Algeo, Lacie A" 
Subject: [Histonet] C4d 
To: 
Message-ID:
	<6A73DCDB9E46D2489D4001B4001C92AE012C4349@IRMEXCH05.irm.inhs.org>
Content-Type: text/plain;	charset="us-ascii"

Hi Everyone,
Does anyone know of a FITC C4d that is ASR or IVD?  All I have been able
to find is RUO.  Also, is anyone using something different to show
rejection in transplant kidneys?
Thank you,
Lacie

Lacie Algeo, HTL, MP(ASCP)
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
Spokane, WA 99220
509-474-4418
FAX 509-474-2052
lacie.algeo@providence.org



------------------------------

Message: 12
Date: Wed, 21 Sep 2011 05:45:14 -0500
From: 
Subject: [Histonet] FW: Procedure for SOS11
To: 
Message-ID:
	<0226177269DFDE48A23F40D00634258B15EF6C83EE@NADCWPMSGCMS10.hca.corpad.net>
	
Content-Type: text/plain; charset="us-ascii"



Elizabeth Ann Dickert
Southern Pathology
2333 McCallie Ave.
Chattanooga, TN.
423-493-6904

_____________________________________________
From: Dickert Elizabeth
Sent: Thursday, September 15, 2011 10:35 AM
To: 'histonet-request@lists.utsouthwestern.edu'
Subject: SOX11


Anyone doing a sox11 with good results?
Have Abcam antibody / Ventana equipment (ultraview detection)

Elizabeth Ann Dickert
Southern Pathology
2333 McCallie Ave.
Chattanooga, TN.
423-493-6904
elizabeth.dickert@hcahealthcare.com



------------------------------

Message: 13
Date: Wed, 21 Sep 2011 12:55:47 +0000
From: "Perry, Margaret" 
Subject: [Histonet] unstructured proteins
To: "ihcrg@googlegroups.com" ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<25F4FBA34BE9D142964ECC4525B82AEE012722@SDSU-EX03.jacks.local>
Content-Type: text/plain; charset="us-ascii"

Have any of you worked with the unstructured proteins?  Do they form the same crosslinks when fixed with formalin?  Would antigen retrieval be of any use?

Margaret Perry HT(ASCP)
Dept of Veterinary and  Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638



------------------------------

Message: 14
Date: Wed, 21 Sep 2011 13:54:00 +0000
From: "Rathborne, Toni" 
Subject: RE: [Histonet] used microscope vendors
To: "'Caroline Bass'" ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<3AD061FE740D464FAC7BF6B5CFB7570711F56F73@SMCMAIL01.somerset-healthcare.com>
	
Content-Type: text/plain; charset="us-ascii"

What part of the country are you in?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Bass
Sent: Tuesday, September 20, 2011 5:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] used microscope vendors

Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out. 

Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units.

Thanks,

Caroline
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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event listings, health information and more.



------------------------------

Message: 15
Date: Wed, 21 Sep 2011 10:10:59 -0400
From: "Sara Baldwin/mhhcc.org" 
Subject: [Histonet] Napsin A controls
To: 
Message-ID:
	
	
Content-Type: text/plain;	charset=ISO-8859-1

Hi Histonetters
Are there any other controls for Napsin A other than Lung adenocarcinoma?

Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-996-0210, 0216,  Fax 812-996-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.


------------------------------

Message: 16
Date: Wed, 21 Sep 2011 07:12:40 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] used microscope vendors
To: histonet@lists.utsouthwestern.edu, Caroline Bass
	
Message-ID:
	<1316614360.28378.YahooMailClassic@web65703.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Try eBay. I have seen good instruments for sale there.
Ren? J.

--- On Tue, 9/20/11, Caroline Bass  wrote:


From: Caroline Bass 
Subject: [Histonet] used microscope vendors
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 20, 2011, 5:30 PM


Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out. 

Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units.

Thanks,

Caroline
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 17
Date: Wed, 21 Sep 2011 09:15:32 -0500
From: "Mike Pence" 
Subject: RE: [Histonet] Napsin A controls
To: "Sara Baldwin/mhhcc.org" ,
	
Message-ID:
	<661949901A768E4F9CC16D8AF8F2838C03974CBD@is-e2k3.grhs.net>
Content-Type: text/plain;	charset="US-ASCII"

Kidney

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara
Baldwin/mhhcc.org
Sent: Wednesday, September 21, 2011 9:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Napsin A controls


Hi Histonetters
Are there any other controls for Napsin A other than Lung
adenocarcinoma?

Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-996-0210, 0216,  Fax 812-996-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 18
Date: Wed, 21 Sep 2011 08:22:15 -0700
From: Tieuvi Nguyen 
Subject: [Histonet] Paid Survey ($50) Opportunity - Digital Pathology
To: "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<61C52EFB4F0AF8498D9ECADA6796B46620E26EDE@VA3DIAXVS3D1.RED001.local>
Content-Type: text/plain; charset="us-ascii"

Hi Everyone,

I had sent a note out to the group about a month ago inviting participants to take part in phone interviews to discuss Digital Pathology technology.  We are now following up those interviews with an online survey on the same subject.  We are particularly interested in individuals that work in high volume commercial or reference labs (i.e. process more than 100,000 slides a year, and process more than 5,000 IHC slides a year).  You do not have to be a current user of digital pathology to participate.  The survey will begin with a few questions to determine if you qualify for the survey.  If you qualify and complete the survey you will receive an honoraria in the form of a $50 American Express gift card for your participation.  All answers will remain anonymous.

The survey should take about 15-20 minutes to complete.

You can access the survey here:

http://www.keysurvey.com/survey/387885/297f/

Please feel free to contact me if you have any questions.


Tieuvi Nguyen, PhD  /  Kaiser Associates, Inc.
M +1 202 506 0775  /  tnguyen@kaiserassociates.com
1201 Connecticut Ave NW, 12th Floor  /  Washington, DC  20036  USA
www.kaiserassociates.com

------------------------------

Message: 19
Date: Wed, 21 Sep 2011 10:07:31 -0600
From: "Breeden, Sara" 
Subject: [Histonet] Klotz Solution
To: 
Message-ID:
	<4D14F0FC9316DD41972D5F03C070908B051DFACC@nmdamailsvr.nmda.ad.nmsu.edu>
	
Content-Type: text/plain;	charset="us-ascii"

I've just "googled" and "histosearched" for Klotz solution but cannot
find anything that doesn't require chloral hydrate.  Has anyone made a
Big Breakthrough in making Klotz that can be made without that pesky
controlled substance?  Any help will be appreciated...who knew the
recipe joined the Witness Protection Program!

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 



------------------------------

Message: 20
Date: Wed, 21 Sep 2011 09:29:07 -0700
From: "Heckford, Karen - SMMC-SF" 
Subject: [Histonet] Urgent!! Need per diem
To: 
Message-ID:
	<9ECF174E7DA83046BE6EBFDE009E28A388C456@CHW-MSG-301.chw.edu>
Content-Type: text/plain;	charset="us-ascii"

I am looking for a Certified ASCP HistoTech. that is local to the San
Francisco Bay Area.  I need this person to cover me while I am out
having knee surgery.  I will be out 2-6 weeks probably more like 4 weeks
starting October 5th.  But need somebody by October 3rd to do some
training.  

 

You will need to know IHC and Special stains and be able to work
independently because I am the Chief, Cook and Bottle washer here.  

 

Thanks,

 

Karen Heckford HT ASCP CE

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-668-1000 ext. 6167Caution:  This email message, including all
content and attachments, is CONFIDENTIAL and may be of a nature that is
LEGALLY PRIVILEGED.  The information contained in this email message is
intended only for the use of the recipient(s) named above. If the reader
of this message is not the intended recipient or an agent responsible
for delivering it to the intended recipient, you have received this
document in error.  Any further review, dissemination, distribution, or
copying of this message is strictly prohibited.  If you have received
this communication in error, please notify us  immediately by reply
email.  Thank you."



 

 



------------------------------

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 94, Issue 24
****************************************

From Margaret.Perry <@t> sdstate.edu  Wed Sep 21 12:54:05 2011
From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret)
Date: Wed Sep 21 12:54:10 2011
Subject: [Histonet] RE: klotz solution
Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE012BC9@SDSU-EX03.jacks.local>

We have the same problem.  All we do now is make up the solution and leave out the Chloral Hydrate.  I haven't talked to the pathologist about it so I don't know if it worked OK.

Margaret Perry HT(ASCP) 
Dept of Veterinary and  Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638



I've just "googled" and "histosearched" for Klotz solution but cannot
find anything that doesn't require chloral hydrate.  Has anyone made a
Big Breakthrough in making Klotz that can be made without that pesky
controlled substance?  Any help will be appreciated...who knew the
recipe joined the Witness Protection Program!

Sally Breeden, HT(ASCP)

From aferullo <@t> celgene.com  Wed Sep 21 12:55:21 2011
From: aferullo <@t> celgene.com (Andrea Ferullo (non-Celgene))
Date: Wed Sep 21 12:55:27 2011
Subject: [Histonet] activated microglia antibody
Message-ID: <24701B0EF4127F4FA262751AA9674EEA029D6E6294F5@SUMEXPRDMB03.celgene.com>

Hello,

We are trying to order activated rat anti-mouse microglia antibody (1:200) from BD Pharmingen but are unable to find the correct catalog number.  Does anyone know where I can find it to order it?  Thanks.


Andrea

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CONFIDENTIAL AND MAY CONTAIN LEGALLY PRIVILEGED
INFORMATION INTENDED ONLY FOR THE USE OF THE INDIVIDUAL
OR INDIVIDUALS NAMED ABOVE. 
If the reader is not the intended recipient, or the
employee or agent responsible to deliver it to the
intended recipient, you are hereby notified that any
dissemination, distribution or copying of this
communication is strictly prohibited. If you have
received this communication in error, please reply to the
sender to notify us of the error and delete the original
message. Thank You.
********************************************************* 

From Elizabeth.Dickert <@t> hcahealthcare.com  Wed Sep 21 13:22:08 2011
From: Elizabeth.Dickert <@t> hcahealthcare.com (Elizabeth.Dickert@hcahealthcare.com)
Date: Wed Sep 21 13:22:14 2011
Subject: [Histonet] RE: Procedure for SOX11
Message-ID: <0226177269DFDE48A23F40D00634258B15EF6C8EFC@NADCWPMSGCMS10.hca.corpad.net>

Sorry fellow Histotechs, that is AB-SOX11

Elizabeth Ann Dickert
Southern Pathology
2333 McCallie Ave.
Chattanooga, TN.
423-493-6904

_____________________________________________
From: Dickert Elizabeth
Sent: Wednesday, September 21, 2011 6:45 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: FW: Procedure for SOS11




Elizabeth Ann Dickert
Southern Pathology
2333 McCallie Ave.
Chattanooga, TN.
423-493-6904

_____________________________________________
From: Dickert Elizabeth
Sent: Thursday, September 15, 2011 10:35 AM
To: 'histonet-request@lists.utsouthwestern.edu'
Subject: SOX11


Anyone doing a sox11 with good results?
Have Abcam antibody / Ventana equipment (ultraview detection)

Elizabeth Ann Dickert
Southern Pathology
2333 McCallie Ave.
Chattanooga, TN.
423-493-6904
elizabeth.dickert@hcahealthcare.com

From leticia.figliuolo <@t> roche.com  Wed Sep 21 13:25:26 2011
From: leticia.figliuolo <@t> roche.com (Figliuolo, Leticia)
Date: Wed Sep 21 13:25:59 2011
Subject: [Histonet] Posters NSH
Message-ID: <069E6CF048B915488720412DAFD1474D041B19EF47@RNUMSEM702.nala.roche.com>

Hello everybody,

Does anybody knows who won the poster session this year at the NSH in Cincinnati? I had to leave before they make the announcement...

Thank you!

Leticia Figliuolo
Non- Clinical Safety
Hoffmann La Roche
Nutley, NJ 07110
Tel. 973 235-3980
Fax. 973 235-4710
e-mail: leticia.figliuolo@roche.com


From jenniferl <@t> slonepartners.com  Wed Sep 21 13:28:05 2011
From: jenniferl <@t> slonepartners.com (Jennifer Lemasters)
Date: Wed Sep 21 13:28:09 2011
Subject: [Histonet] Histotechnologist opportunity in the Washington, DC area
Message-ID: <5D03F1B0-A5CE-4186-969B-10A23ACEAD98@slonepartners.com>

If anyone is interested in learning more about a Histotechnologist opportunity in the Washington, DC area, please contact me at jenniferl@slonepartners.com or 877-564-1046. You would be working 5:30 - 2:30 in a cutting edge laboratory with a unique and varied workload.

Best regards,

Jennifer
jenniferl@slonepartners.com

SLONEPARTNERS
JENNIFER LEMASTERS - EXECUTIVE RECRUITER

Corporate Headquarters
1521 Alton Road  #638
Miami Beach, Florida 33139

TOLL FREE: 	877.564.1046
DIRECT:  		412.788.2389

www.slonepartners.com


From MHuth <@t> baymedical.org  Wed Sep 21 15:28:35 2011
From: MHuth <@t> baymedical.org (Huth, Myra)
Date: Wed Sep 21 15:28:50 2011
Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 24
In-Reply-To: 
Message-ID: <4565810021302841A3599A8F7CCE820F0A60587F@BMCEXCHVS1.corp.baymedical.org>

Kidney works great for Napsin
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Wednesday, September 21, 2011 12:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 94, Issue 24

Send Histonet mailing list submissions to
	histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
	histonet-request@lists.utsouthwestern.edu

You can reach the person managing the list at
	histonet-owner@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Legal Case SOP (Ann Angelo)
   2. RE: Uni-trieve, antigen retrieval for immunofluorescence
      (sdysart@mirnarx.com)
   3. cost of an H&E (Jennifer MacDonald)
   4. RE: cost of an H&E (Burton, Lynn)
   5. Effect of Freezing on Unfixed GFP (Andrew Coleman)
   6. Re: Tissue handling at embedding (Rene J Buesa)
   7. Re: cost of an H&E (Rene J Buesa)
   8. Tissue handling at embedding (Tim Higgins)
   9. RE: Uni-trieve, antigen retrieval for immunofluorescence
      (Andrea Marion)
  10. used microscope vendors (Caroline Bass)
  11. C4d  (Algeo, Lacie A)
  12. FW: Procedure for SOS11 (Elizabeth.Dickert@hcahealthcare.com)
  13. unstructured proteins (Perry, Margaret)
  14. RE: used microscope vendors (Rathborne, Toni)
  15. Napsin A controls (Sara Baldwin/mhhcc.org)
  16. Re: used microscope vendors (Rene J Buesa)
  17. RE: Napsin A controls (Mike Pence)
  18. Paid Survey ($50) Opportunity - Digital Pathology (Tieuvi Nguyen)
  19. Klotz Solution (Breeden, Sara)
  20. Urgent!! Need per diem (Heckford, Karen - SMMC-SF)


----------------------------------------------------------------------

Message: 1
Date: Tue, 20 Sep 2011 13:20:14 -0400 (EDT)
From: Ann Angelo 
Subject: [Histonet] Legal Case SOP
To: histonet@lists.utsouthwestern.edu
Message-ID: <8CE45D0B1BF937B-2198-21BC4@webmail-d008.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

Can anyone share their SOP regarding the handling of legal cases (I.e. if a specimen is being requested from an outside source which is not the patient).  Thank you, Ann




------------------------------

Message: 2
Date: Tue, 20 Sep 2011 12:49:06 -0500
From: 
Subject: RE: [Histonet] Uni-trieve, antigen retrieval for
	immunofluorescence
To: ,	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

I actually attended a class they put on a few months ago where they used
Unitrieve.  It works really well.  It doesn't seem to produce any kind
of background and yes, it is used at much lower temps.  However the
retrieval process takes a little longer, but...you can put it in an even
lower temp. water bath overnight, come in to work in the morning, and
your slides are reading to go.
Contact Zara over there and she might be able to hook you up with a
sample.
Good Luck!!

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea
Marion
Sent: Tuesday, September 20, 2011 11:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence

Hello all,

Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported
to
be a universal antigen retrieval solution that can be used at lower
temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear
antigens):

http://innvx.com/unitrievepage.html

The company claims that the reagent is a universal retrieval solution
for
all antibodies and tissues (which is silly of course - how could they
know?). Does anyone have any experience with the product?

I am interested because I see that increased heat during antigen
retrieval
causes greater tissue autofluorescence during immunofluorescence
stainings. My current protocol is to use 20 minutes at 90-95 C on a hot
plate using sodium citrate buffer. Does anyone else either Uni-trieve or
a
different reagent/protocol for immunofluorescence stainings?

Thanks,

Andrea

Andrea Marion
Graduate Student
University of Illinois at Chicago


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 3
Date: Tue, 20 Sep 2011 11:23:55 -0700
From: Jennifer MacDonald 
Subject: [Histonet] cost of an H&E
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset="US-ASCII"

Has anyone calculated the approximate cost of an H&E, materials only, no 
labor?  Including the cost of the cassette, paraffin, slide, hematoxylin, 
eosin and staining reagents?
Thanks,
Jennifer MacDonald
Mt. San Antonio College

------------------------------

Message: 4
Date: Tue, 20 Sep 2011 13:30:12 -0500
From: "Burton, Lynn" 
Subject: RE: [Histonet] cost of an H&E
To: Jennifer MacDonald ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<4A6E2CACA1E017408EBA1B9911952CC00643DD12DF@IL084EXMBX214.illinois.gov>
	
Content-Type: text/plain; charset="us-ascii"

We calculated that to be right around $4.05 per slide. That does not include labor.

Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald [JMacDonald@mtsac.edu]
Sent: Tuesday, September 20, 2011 1:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cost of an H&E

Has anyone calculated the approximate cost of an H&E, materials only, no
labor?  Including the cost of the cassette, paraffin, slide, hematoxylin,
eosin and staining reagents?
Thanks,
Jennifer MacDonald
Mt. San Antonio College
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 5
Date: Tue, 20 Sep 2011 15:50:51 -0400
From: Andrew Coleman 
Subject: [Histonet] Effect of Freezing on Unfixed GFP
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1

We have performed lentiviral injections with a turboGreenFluorescentProtein
(tGFP) into the brains of rats for an RNAi experiment. We did not fix the
tissue but froze in isopentane on dry ice and stored the tissue at -80 deg
Celsius.

We want to use some sections for a reference of the injection site (GFP
fluorescence) relative to the brain morphology (Neurotracer staining) in
order to selecitvely laser microdissect (LMD) infected cells from other
sections.

Our initial studies showed that fixation of sections on slides in 4%
paraformaledhyde (PFA) at 4 deg C for 5 minutes provided tGFP flourescence
comparable to that found in perfused tissue, but lately we see very weak or
no fluorescence with this procedure, even when increasing the time.

The only difference I can think of that we saw best results soon after the
brains were removed (ie 1 to 2 weeks) and now we are having trouble
visualizing the tGFP ~2 months of the brains being stored at -80 degrees
celsius.

Does anyone have experience with or an explanation of fluorescence
decreasing with a frozen, unfixed GFP over time?

In the future we plan on doing these experiments including a perfusion with
a low conc of PFA (to preserve RNA integrity) but for now we are trying to
figure out how to identify the GFP-positive cells in the animals we have
already performed surgery on. Another option would anti-tGFP IHC, but we
would prefer at this point to use on slide-fixed sections as a references
for our LMD.

Thanks!  -Andrew


------------------------------

Message: 6
Date: Tue, 20 Sep 2011 12:51:45 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] Tissue handling at embedding
To: "histonet@lists.utsouthwestern.edu"
	,	TimothyMorken
	
Message-ID:
	<1316548305.7251.YahooMailClassic@web65716.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

This is how we do it:
1- the person grossing will dictate how many pieces should go into each cassette
2- the person cassetting will check that information against the vial with the pieces and write down in the cassettes summary form the number of pieces
3- the person embedding checks the pieces in the cassette after processing against the cassettes log.
If any piece is missing between any two steps, it will be looked for. Sometimes is small and is in the vial before cassetting, or small and in the retort.
Any irregularity is documented in the QA log.
Ren? J.

--- On Tue, 9/20/11, Morken, Timothy  wrote:


From: Morken, Timothy 
Subject: [Histonet] Tissue handling at embedding
To: "histonet@lists.utsouthwestern.edu" 
Date: Tuesday, September 20, 2011, 12:44 PM


What procedures do you have in place to prevent tissue loss at embedding? What do you do if tissue appears to have been lost?

And do you clean embedding molds before each reuse, or after one-days use (which may be many re-uses)?


Thanks for all info!


Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA

415.514.6042 (office)
415.885.7409 Fax
tim.morken@ucsfmedctr.org


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 7
Date: Tue, 20 Sep 2011 12:53:12 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] cost of an H&E
To: histonet@lists.utsouthwestern.edu,	Jennifer MacDonald
	
Message-ID:
	<1316548392.93704.YahooMailClassic@web65710.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Under separate cover I am sending something I wrote about this subject.
Ren? J.

--- On Tue, 9/20/11, Jennifer MacDonald  wrote:


From: Jennifer MacDonald 
Subject: [Histonet] cost of an H&E
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 20, 2011, 2:23 PM


Has anyone calculated the approximate cost of an H&E, materials only, no 
labor?  Including the cost of the cassette, paraffin, slide, hematoxylin, 
eosin and staining reagents?
Thanks,
Jennifer MacDonald
Mt. San Antonio College
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 8
Date: Tue, 20 Sep 2011 15:03:06 -0500
From: "Tim Higgins" 
Subject: [Histonet] Tissue handling at embedding
To: 
Message-ID: <001101cc77d0$50b7afa0$e001a8c0@cdd.loc>
Content-Type: text/plain;	charset="iso-8859-1"

I have worked at labs that reuse molds and ones that clean between uses.
Guess it all depends on what your definition of a "clean" mold is.  If you
have the time and space, not reusing the molds is best practice but not
always practical.

Thanks,

Tim


Message: 10
Date: Tue, 20 Sep 2011 09:44:26 -0700
From: "Morken, Timothy" 
Subject: [Histonet] Tissue handling at embedding
To: "histonet@lists.utsouthwestern.edu"

Message-ID:
<8D7C2D242DBD45498006B21122072BF85E2AEE21@MCINFRWEM003.ucsfmedicalcenter.org
>

Content-Type: text/plain; charset=us-ascii

What procedures do you have in place to prevent tissue loss at embedding?
What do you do if tissue appears to have been lost?

And do you clean embedding molds before each reuse, or after one-days use
(which may be many re-uses)?


Thanks for all info!


Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA

415.514.6042 (office)
415.885.7409 Fax
tim.morken@ucsfmedctr.org






------------------------------

Message: 9
Date: Tue, 20 Sep 2011 15:19:48 -0500
From: "Andrea Marion" 
Subject: RE: [Histonet] Uni-trieve, antigen retrieval for
	immunofluorescence
To: sdysart@mirnarx.com
Cc: histonet@lists.utsouthwestern.edu, amario3@uic.edu
Message-ID:
	
Content-Type: text/plain;charset=iso-8859-1

Hi Sarah,

Thanks for the info! Have you used Unitrieve for immunofluorescence? I am
wondering whether I will see a reduction in the autofluorescence caused by
heating? I will request a sample to try and report back to all.

Andrea

Andrea Marion
Graduate Student
University of Illinois at Chicago

On Tue, September 20, 2011 12:49 pm, sdysart@mirnarx.com wrote:
> I actually attended a class they put on a few months ago where they used
> Unitrieve.  It works really well.  It doesn't seem to produce any kind
> of background and yes, it is used at much lower temps.  However the
> retrieval process takes a little longer, but...you can put it in an even
> lower temp. water bath overnight, come in to work in the morning, and
> your slides are reading to go.
> Contact Zara over there and she might be able to hook you up with a
> sample.
> Good Luck!!
>
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas  78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea
> Marion
> Sent: Tuesday, September 20, 2011 11:59 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence
>
> Hello all,
>
> Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported
> to
> be a universal antigen retrieval solution that can be used at lower
> temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear
> antigens):
>
> http://innvx.com/unitrievepage.html
>
> The company claims that the reagent is a universal retrieval solution
> for
> all antibodies and tissues (which is silly of course - how could they
> know?). Does anyone have any experience with the product?
>
> I am interested because I see that increased heat during antigen
> retrieval
> causes greater tissue autofluorescence during immunofluorescence
> stainings. My current protocol is to use 20 minutes at 90-95 C on a hot
> plate using sodium citrate buffer. Does anyone else either Uni-trieve or
> a
> different reagent/protocol for immunofluorescence stainings?
>
> Thanks,
>
> Andrea
>
> Andrea Marion
> Graduate Student
> University of Illinois at Chicago
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>





------------------------------

Message: 10
Date: Tue, 20 Sep 2011 17:30:26 -0400
From: Caroline Bass 
Subject: [Histonet] used microscope vendors
To: histonet@lists.utsouthwestern.edu
Message-ID: <49365679-58DD-48DE-80D5-0355BBA95515@buffalo.edu>
Content-Type: text/plain; charset=us-ascii

Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out. 

Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units.

Thanks,

Caroline


------------------------------

Message: 11
Date: Tue, 20 Sep 2011 16:21:05 -0700
From: "Algeo, Lacie A" 
Subject: [Histonet] C4d 
To: 
Message-ID:
	<6A73DCDB9E46D2489D4001B4001C92AE012C4349@IRMEXCH05.irm.inhs.org>
Content-Type: text/plain;	charset="us-ascii"

Hi Everyone,
Does anyone know of a FITC C4d that is ASR or IVD?  All I have been able
to find is RUO.  Also, is anyone using something different to show
rejection in transplant kidneys?
Thank you,
Lacie

Lacie Algeo, HTL, MP(ASCP)
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
Spokane, WA 99220
509-474-4418
FAX 509-474-2052
lacie.algeo@providence.org



------------------------------

Message: 12
Date: Wed, 21 Sep 2011 05:45:14 -0500
From: 
Subject: [Histonet] FW: Procedure for SOS11
To: 
Message-ID:
	<0226177269DFDE48A23F40D00634258B15EF6C83EE@NADCWPMSGCMS10.hca.corpad.net>
	
Content-Type: text/plain; charset="us-ascii"



Elizabeth Ann Dickert
Southern Pathology
2333 McCallie Ave.
Chattanooga, TN.
423-493-6904

_____________________________________________
From: Dickert Elizabeth
Sent: Thursday, September 15, 2011 10:35 AM
To: 'histonet-request@lists.utsouthwestern.edu'
Subject: SOX11


Anyone doing a sox11 with good results?
Have Abcam antibody / Ventana equipment (ultraview detection)

Elizabeth Ann Dickert
Southern Pathology
2333 McCallie Ave.
Chattanooga, TN.
423-493-6904
elizabeth.dickert@hcahealthcare.com



------------------------------

Message: 13
Date: Wed, 21 Sep 2011 12:55:47 +0000
From: "Perry, Margaret" 
Subject: [Histonet] unstructured proteins
To: "ihcrg@googlegroups.com" ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<25F4FBA34BE9D142964ECC4525B82AEE012722@SDSU-EX03.jacks.local>
Content-Type: text/plain; charset="us-ascii"

Have any of you worked with the unstructured proteins?  Do they form the same crosslinks when fixed with formalin?  Would antigen retrieval be of any use?

Margaret Perry HT(ASCP)
Dept of Veterinary and  Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638



------------------------------

Message: 14
Date: Wed, 21 Sep 2011 13:54:00 +0000
From: "Rathborne, Toni" 
Subject: RE: [Histonet] used microscope vendors
To: "'Caroline Bass'" ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<3AD061FE740D464FAC7BF6B5CFB7570711F56F73@SMCMAIL01.somerset-healthcare.com>
	
Content-Type: text/plain; charset="us-ascii"

What part of the country are you in?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Bass
Sent: Tuesday, September 20, 2011 5:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] used microscope vendors

Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out. 

Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units.

Thanks,

Caroline
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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------------------------------

Message: 15
Date: Wed, 21 Sep 2011 10:10:59 -0400
From: "Sara Baldwin/mhhcc.org" 
Subject: [Histonet] Napsin A controls
To: 
Message-ID:
	
	
Content-Type: text/plain;	charset=ISO-8859-1

Hi Histonetters
Are there any other controls for Napsin A other than Lung adenocarcinoma?

Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-996-0210, 0216,  Fax 812-996-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.


------------------------------

Message: 16
Date: Wed, 21 Sep 2011 07:12:40 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] used microscope vendors
To: histonet@lists.utsouthwestern.edu, Caroline Bass
	
Message-ID:
	<1316614360.28378.YahooMailClassic@web65703.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Try eBay. I have seen good instruments for sale there.
Ren? J.

--- On Tue, 9/20/11, Caroline Bass  wrote:


From: Caroline Bass 
Subject: [Histonet] used microscope vendors
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 20, 2011, 5:30 PM


Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out. 

Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units.

Thanks,

Caroline
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 17
Date: Wed, 21 Sep 2011 09:15:32 -0500
From: "Mike Pence" 
Subject: RE: [Histonet] Napsin A controls
To: "Sara Baldwin/mhhcc.org" ,
	
Message-ID:
	<661949901A768E4F9CC16D8AF8F2838C03974CBD@is-e2k3.grhs.net>
Content-Type: text/plain;	charset="US-ASCII"

Kidney

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara
Baldwin/mhhcc.org
Sent: Wednesday, September 21, 2011 9:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Napsin A controls


Hi Histonetters
Are there any other controls for Napsin A other than Lung
adenocarcinoma?

Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-996-0210, 0216,  Fax 812-996-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 18
Date: Wed, 21 Sep 2011 08:22:15 -0700
From: Tieuvi Nguyen 
Subject: [Histonet] Paid Survey ($50) Opportunity - Digital Pathology
To: "histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<61C52EFB4F0AF8498D9ECADA6796B46620E26EDE@VA3DIAXVS3D1.RED001.local>
Content-Type: text/plain; charset="us-ascii"

Hi Everyone,

I had sent a note out to the group about a month ago inviting participants to take part in phone interviews to discuss Digital Pathology technology.  We are now following up those interviews with an online survey on the same subject.  We are particularly interested in individuals that work in high volume commercial or reference labs (i.e. process more than 100,000 slides a year, and process more than 5,000 IHC slides a year).  You do not have to be a current user of digital pathology to participate.  The survey will begin with a few questions to determine if you qualify for the survey.  If you qualify and complete the survey you will receive an honoraria in the form of a $50 American Express gift card for your participation.  All answers will remain anonymous.

The survey should take about 15-20 minutes to complete.

You can access the survey here:

http://www.keysurvey.com/survey/387885/297f/

Please feel free to contact me if you have any questions.


Tieuvi Nguyen, PhD  /  Kaiser Associates, Inc.
M +1 202 506 0775  /  tnguyen@kaiserassociates.com
1201 Connecticut Ave NW, 12th Floor  /  Washington, DC  20036  USA
www.kaiserassociates.com

------------------------------

Message: 19
Date: Wed, 21 Sep 2011 10:07:31 -0600
From: "Breeden, Sara" 
Subject: [Histonet] Klotz Solution
To: 
Message-ID:
	<4D14F0FC9316DD41972D5F03C070908B051DFACC@nmdamailsvr.nmda.ad.nmsu.edu>
	
Content-Type: text/plain;	charset="us-ascii"

I've just "googled" and "histosearched" for Klotz solution but cannot
find anything that doesn't require chloral hydrate.  Has anyone made a
Big Breakthrough in making Klotz that can be made without that pesky
controlled substance?  Any help will be appreciated...who knew the
recipe joined the Witness Protection Program!

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 



------------------------------

Message: 20
Date: Wed, 21 Sep 2011 09:29:07 -0700
From: "Heckford, Karen - SMMC-SF" 
Subject: [Histonet] Urgent!! Need per diem
To: 
Message-ID:
	<9ECF174E7DA83046BE6EBFDE009E28A388C456@CHW-MSG-301.chw.edu>
Content-Type: text/plain;	charset="us-ascii"

I am looking for a Certified ASCP HistoTech. that is local to the San
Francisco Bay Area.  I need this person to cover me while I am out
having knee surgery.  I will be out 2-6 weeks probably more like 4 weeks
starting October 5th.  But need somebody by October 3rd to do some
training.  

 

You will need to know IHC and Special stains and be able to work
independently because I am the Chief, Cook and Bottle washer here.  

 

Thanks,

 

Karen Heckford HT ASCP CE

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-668-1000 ext. 6167Caution:  This email message, including all
content and attachments, is CONFIDENTIAL and may be of a nature that is
LEGALLY PRIVILEGED.  The information contained in this email message is
intended only for the use of the recipient(s) named above. If the reader
of this message is not the intended recipient or an agent responsible
for delivering it to the intended recipient, you have received this
document in error.  Any further review, dissemination, distribution, or
copying of this message is strictly prohibited.  If you have received
this communication in error, please notify us  immediately by reply
email.  Thank you."



 

 



------------------------------

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End of Histonet Digest, Vol 94, Issue 24
****************************************
 
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From mtitford <@t> aol.com  Wed Sep 21 15:20:28 2011
From: mtitford <@t> aol.com (mtitford@aol.com)
Date: Wed Sep 21 15:51:23 2011
Subject: [Histonet] Lagal cases SOP
Message-ID: <8CE46B309D064DB-7F8-32867@angweb-usd004.sysops.aol.com>


Ann Angelo asks about releasing tissues in legal cases.

I guess how you do it depends on your state and its laws, and what your own facility lawyers require.

At our facility in the State of Alabama, to release tissues and/or medical records we require either a release signed by the patient, a subpoena, or a court order.  If the patient has passed away, and the next of kin is making the request, we need documentsation they are legally the first next of kin.

THEN, we let the lawyer in our risk management department check it over. After she has blessed it, we can release the material.

A lot of requests are made by firms who go round vacuuming up every item they can for other law firms.

We do not release blocks, or original slides, but can release recuts.

Hope this helps.

Michael Titford
USA Pathology
Mobile AL USA



From sadey <@t> hotmail.ca  Thu Sep 22 05:16:25 2011
From: sadey <@t> hotmail.ca (Sheila Adey)
Date: Thu Sep 22 05:16:30 2011
Subject: [Histonet] OR electronically submitting specimen orders
Message-ID: 


Hello:
Is anyone using a computer system to have the OR submit their specimen requests electronically? Also, I am interested in specimen tracking by having the specimen scanned at the pick up point instead of using a paper log.
Any suggestions would be great 
:)
Sheila 		 	   		  
From mcauliff <@t> umdnj.edu  Thu Sep 22 08:24:48 2011
From: mcauliff <@t> umdnj.edu (Geoff McAuliffe)
Date: Thu Sep 22 08:24:11 2011
Subject: [Histonet] activated microglia antibody
In-Reply-To: <24701B0EF4127F4FA262751AA9674EEA029D6E6294F5@SUMEXPRDMB03.celgene.com>
References: <24701B0EF4127F4FA262751AA9674EEA029D6E6294F5@SUMEXPRDMB03.celgene.com>
Message-ID: <4E7B3720.8020608@umdnj.edu>

You could call the company?

Geoff


On 9/21/2011 1:55 PM, Andrea Ferullo (non-Celgene) wrote:
> Hello,
>
> We are trying to order activated rat anti-mouse microglia antibody (1:200) from BD Pharmingen but are unable to find the correct catalog number.  Does anyone know where I can find it to order it?  Thanks.
>
>
> Andrea
>
> *********************************************************
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>
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>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff@umdnj.edu
**********************************************



From flnails <@t> texaschildrens.org  Thu Sep 22 09:18:48 2011
From: flnails <@t> texaschildrens.org (Nails, Felton)
Date: Thu Sep 22 09:19:01 2011
Subject: [Histonet] Thermo Shandon Histobathe
In-Reply-To: <1316614360.28378.YahooMailClassic@web65703.mail.ac4.yahoo.com>
References: <49365679-58DD-48DE-80D5-0355BBA95515@buffalo.edu>
	<1316614360.28378.YahooMailClassic@web65703.mail.ac4.yahoo.com>
Message-ID: 

 
What are facilities using to replace their histobath in their frozen labs, since thermo no longer manufactures the histobath?

_______________________________________________
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

______________________________________________________________________
CONFIDENTIALITY NOTICE:
 The information in this e-mail may be confidential and/or
 privileged.  If you are not the intended recipient or an
 authorized representative of the intended recipient, you
 are hereby notified that any review, dissemination, or
 copying of this e-mail and its attachments, if any, or
 the information contained herein is prohibited.  If you
 have received this e-mail in error, please immediately
 notify the sender by return e-mail and delete this e-mail
 from your computer system.  Thank you.
______________________________________________________________________

From sdysart <@t> mirnarx.com  Thu Sep 22 09:32:28 2011
From: sdysart <@t> mirnarx.com (sdysart@mirnarx.com)
Date: Thu Sep 22 09:32:31 2011
Subject: [Histonet] Thermo Shandon Histobathe
In-Reply-To: 
References: <49365679-58DD-48DE-80D5-0355BBA95515@buffalo.edu><1316614360.28378.YahooMailClassic@web65703.mail.ac4.yahoo.com>
	
Message-ID: 

Liquid nitrogen in a carboy?  Why do you need a fancy machine just to
snap freeze?  Sounds like Thermo trying to rip people off??

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails,
Felton
Sent: Thursday, September 22, 2011 9:19 AM
To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Thermo Shandon Histobathe

 
What are facilities using to replace their histobath in their frozen
labs, since thermo no longer manufactures the histobath?

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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 The information in this e-mail may be confidential and/or
 privileged.  If you are not the intended recipient or an
 authorized representative of the intended recipient, you
 are hereby notified that any review, dissemination, or
 copying of this e-mail and its attachments, if any, or
 the information contained herein is prohibited.  If you
 have received this e-mail in error, please immediately
 notify the sender by return e-mail and delete this e-mail
 from your computer system.  Thank you.
______________________________________________________________________

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From relia1 <@t> earthlink.net  Thu Sep 22 09:43:00 2011
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Thu Sep 22 09:43:09 2011
Subject: [Histonet] RELIA Hot Histology Job Alert - Exciting opportunities
	in NC, CA, MA and MI
Message-ID: <37A7F1BB1A60474C923B1E53CFE7AFB9@ownerf1abaad51>

Hi Histonetters!!!
I hope everybody is having a great day!  I have a couple of positions
that I want to tell you about that I am really excited about.
Here are the details-
Histotech/PA - Charlotte, NC
working with one of my best clients that is in need of a full time
permanent ASCP certified histotech. ASCP HT or HTL and at least 3 years
of routine histology experience is required. IHC experience and
experience grossing large and small specimens is preferred. QIHC is a
plus. This is one of our best clients because I have placed several
people there and they LOVE it. This client has very low turnover, every
position I have filled with them has been because of growth.  Excellent
pay and great benefits, relocation assistance and one of the best groups
of people to work with. 

Mohs Histotech - Opportunity to learn Mohs - Marshall, MI
I am also working with a private dermpath lab located in Marshall, MI.
My client is in need of a Dermpath histotech (Mohs experience a plus)
for a full time M-F dayshift position.  ASCP or eligible with at least 2
yrs experience.  
My client offers excellent compensation and benefits and the opportunity
to learn Mohs. For more information please contact Pam Barker at
866-607-3542 or relia1@earthlink.net 
Keywords histology histologist histotechnician histotechnologist Mohs 
I also have several exciting positions in NY, CA and MA.  If you or
anyone you know would like more information on any of these
opportunities please contact me.  I can be reached at
relia1@earthlink.net or toll free at 866-607-3542.  Thanks-Pam
 
For more

Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.comPamBarkerRELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

From Allison_Scott <@t> hchd.tmc.edu  Thu Sep 22 09:44:26 2011
From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D)
Date: Thu Sep 22 09:44:42 2011
Subject: [Histonet] Audible alarm
Message-ID: <1872B4A455B7974391609AD8034C79FC026DFE7E@LBEXCH01.hchd.local>

Hello to all in histoland.  We recently had a issue withour tissue
processor in regards to a power outage.  Once the popwer came back on
the processor was in a power out stage and did not process our blocks.
Does any one have an external alarm attached to their tissue processor
that would alarm in another area when a problem occurs or a automatic
dialer that would start calling people.  We are using a UPS back up
power box on both of our processors right now.  I'm trying to see which
would be the best way to resolve this issue.  Any help in this matter
will be greatly appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under 
Texas law.  The e-mail is for the use of only the individual or entity named 
above.  If you are not the intended recipient, or any authorized 
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From azdudley <@t> hotmail.com  Thu Sep 22 09:47:19 2011
From: azdudley <@t> hotmail.com (anita dudley)
Date: Thu Sep 22 09:47:24 2011
Subject: [Histonet] new antibody lot
Message-ID: 


how are others handling new lot numbers of antibodies when you receive them in the lab?
thanks for any input.  have a great day!!!
anita dudley
providence hospital
mobile alabama
  		 	   		  
From shive003 <@t> umn.edu  Thu Sep 22 09:52:36 2011
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Thu Sep 22 09:52:43 2011
Subject: [Histonet] new antibody lot
References: 
Message-ID: <67A74ACD0BB44E67BB8D0EC2A56B1A6A@auxs.umn.edu>

Ours are validated and optimized anew, just like if we were working up an 
entirely new antibody.

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003@umn.edu

(Confidentiality Notice: This message, together with any attachments, is 
intended only for the use of the individual or entity to which it is 
addressed and may contain confidential or privileged information. If you 
think you have received this message in error, please advise the sender and 
then delete this message and any attachments immediately.)

----- Original Message ----- 
From: "anita dudley" 
To: 
Sent: Thursday, September 22, 2011 9:47 AM
Subject: [Histonet] new antibody lot



how are others handling new lot numbers of antibodies when you receive them 
in the lab?
thanks for any input.  have a great day!!!
anita dudley
providence hospital
mobile alabama
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Histonet mailing list
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From rjbuesa <@t> yahoo.com  Thu Sep 22 09:55:31 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Sep 22 09:55:34 2011
Subject: [Histonet] Audible alarm
In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFE7E@LBEXCH01.hchd.local>
Message-ID: <1316703331.85964.YahooMailClassic@web65710.mail.ac4.yahoo.com>

The best option is to have a dedicated power supply connected to your hospital emergency generator.
It will have to fail the electricity from the grid and from the hospital to produce what you describe, which is very unlikely.
Ren? J.

--- On Thu, 9/22/11, Scott, Allison D  wrote:


From: Scott, Allison D 
Subject: [Histonet] Audible alarm
To: histonet@lists.utsouthwestern.edu
Date: Thursday, September 22, 2011, 10:44 AM


Hello to all in histoland.? We recently had a issue withour tissue
processor in regards to a power outage.? Once the popwer came back on
the processor was in a power out stage and did not process our blocks.
Does any one have an external alarm attached to their tissue processor
that would alarm in another area when a problem occurs or a automatic
dialer that would start calling people.? We are using a UPS back up
power box on both of our processors right now.? I'm trying to see which
would be the best way to resolve this issue.? Any help in this matter
will be greatly appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.? This e-mail may also be confidential and/or privileged under 
Texas law.? The e-mail is for the use of only the individual or entity named 
above.? If you are not the intended recipient, or any authorized 
representative of the intended recipient, you are hereby notified that any 
review, dissemination or copying of this e-mail and its attachments is 
strictly prohibited.

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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From trathborne <@t> somerset-healthcare.com  Thu Sep 22 09:58:22 2011
From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni)
Date: Thu Sep 22 09:58:33 2011
Subject: [Histonet] RE: Audible alarm
In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFE7E@LBEXCH01.hchd.local>
References: <1872B4A455B7974391609AD8034C79FC026DFE7E@LBEXCH01.hchd.local>
Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F57F4E@SMCMAIL01.somerset-healthcare.com>

We do have an alarm set up to go off in the main lab. Next to the switch to turn off the sound (it's really loud!), we have a list of phone numbers so that a supervisor or one of the histotechs can come in if necessary. We really use this only for a processor malfunction, since all of our critical instruments are connected to the back up generator supply designated by red outlets.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Thursday, September 22, 2011 10:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Audible alarm

Hello to all in histoland.  We recently had a issue withour tissue processor in regards to a power outage.  Once the popwer came back on the processor was in a power out stage and did not process our blocks.
Does any one have an external alarm attached to their tissue processor that would alarm in another area when a problem occurs or a automatic dialer that would start calling people.  We are using a UPS back up power box on both of our processors right now.  I'm trying to see which would be the best way to resolve this issue.  Any help in this matter will be greatly appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system.

To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.  This e-mail may also be confidential and/or privileged under Texas law.  The e-mail is for the use of only the individual or entity named above.  If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited.

_______________________________________________
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From jmcgough <@t> clinlab.com  Thu Sep 22 10:05:36 2011
From: jmcgough <@t> clinlab.com (Jason McGough)
Date: Thu Sep 22 10:05:40 2011
Subject: [Histonet] Audible alarm
In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFE7E@LBEXCH01.hchd.local>
Message-ID: 

We use an external alarm on all of our processors. The alarm is hooked up to
a dialer that calls our pager. We have one of our techs on call then during
after hours. This has been successful. We have had the alarm call out and
delays have been minutes instead of hours. There are other errors that stop
processing, other than power outage, so that is why we went with the dialer.
We do have UPS backup too the dialer and processors are plugged into those
outlets. Our pathologists like it and so do the techs.

Jason McGough HT(ASCP)
Account Representative - Anatomic Pathology
Clinical Laboratory of the Black Hills
2805 5th Street Suite 210
Rapid City, SD 57701
605-343-2267 Ext 127
605-718-3779 (Fax)
jmcgough@clinlab.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Scott,
Allison D
Sent: Thursday, September 22, 2011 8:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Audible alarm


Hello to all in histoland.  We recently had a issue withour tissue
processor in regards to a power outage.  Once the popwer came back on
the processor was in a power out stage and did not process our blocks.
Does any one have an external alarm attached to their tissue processor
that would alarm in another area when a problem occurs or a automatic
dialer that would start calling people.  We are using a UPS back up
power box on both of our processors right now.  I'm trying to see which
would be the best way to resolve this issue.  Any help in this matter
will be greatly appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from
your computer system.

To the extent the information in this e-mail and any attachments contain
protected health information as defined by the Health Insurance Portability
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or
privileged.  This e-mail may also be confidential and/or privileged under
Texas law.  The e-mail is for the use of only the individual or entity named
above.  If you are not the intended recipient, or any authorized
representative of the intended recipient, you are hereby notified that any
review, dissemination or copying of this e-mail and its attachments is
strictly prohibited.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From pathologylab <@t> ymail.com  Thu Sep 22 10:19:58 2011
From: pathologylab <@t> ymail.com (Pathology Lab)
Date: Thu Sep 22 10:20:02 2011
Subject: [Histonet] Sentinel nodule potocols for pathology labs
Message-ID: <1316704798.85548.YahooMailNeo@web121411.mail.ne1.yahoo.com>

Hi? Does anyone can tell me about any protocols for the management of a sentinel nodule, precautions etc. thanks

?
Lcda. Mary V. Guerrero,BS, MBA,HtL ? ? ? ? ? ? ?
Administradora/Coordinadora General
?Pathology Lab.? ? 
55?N. Dr. Basora Edificio M?dico IV?Oficina 206
Mayaguez, Puerto Rico 00680
Tel. 787-834-8202 ?Fax: 787-831-5255



Sra. Dimary Valent?n?????????? Sra. Iris Franqui??????????????? Sra. Myrna Gonz?lez
? Facturaci?n???????? Reportes???????? ? ? Transcripcion


This email may contain confidential health information that is legally privileged.? This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled.? If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited.? If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. .
From laurie.colbert <@t> huntingtonhospital.com  Thu Sep 22 10:31:40 2011
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Thu Sep 22 10:31:44 2011
Subject: [Histonet] Audible alarm
In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFE7E@LBEXCH01.hchd.local>
References: <1872B4A455B7974391609AD8034C79FC026DFE7E@LBEXCH01.hchd.local>
Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2B11F@EXCHANGE3.huntingtonhospital.com>

Our processors are plugged into emergency outlets, so power outages are
rarely a problem.  However, when the processor fails for another reason,
it will alarm in Blood Bank since there is someone in there 24/7.  They
will call me at home and we will actually troubleshoot the problem.
We've had several occasions where a bottle was not pushed in all the
way, and I can walk someone through that over the phone.
Laurie colbert

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Thursday, September 22, 2011 7:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Audible alarm

Hello to all in histoland.  We recently had a issue withour tissue
processor in regards to a power outage.  Once the popwer came back on
the processor was in a power out stage and did not process our blocks.
Does any one have an external alarm attached to their tissue processor
that would alarm in another area when a problem occurs or a automatic
dialer that would start calling people.  We are using a UPS back up
power box on both of our processors right now.  I'm trying to see which
would be the best way to resolve this issue.  Any help in this matter
will be greatly appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain

protected health information as defined by the Health Insurance
Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160
and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential
and/or 
privileged.  This e-mail may also be confidential and/or privileged
under 
Texas law.  The e-mail is for the use of only the individual or entity
named 
above.  If you are not the intended recipient, or any authorized 
representative of the intended recipient, you are hereby notified that
any 
review, dissemination or copying of this e-mail and its attachments is 
strictly prohibited.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From rsrichmond <@t> gmail.com  Thu Sep 22 10:47:10 2011
From: rsrichmond <@t> gmail.com (Bob Richmond)
Date: Thu Sep 22 10:47:15 2011
Subject: [Histonet] Re: Klotz Solution
Message-ID: 

According to the Histosearch link, Klotz solution - whatever that is -
is used to preserve color in specimens.

I'd recommend looking up the Kayserling series of fixatives if that's
what needed.

The chloral hydrate is probably there as a preservative. It could
probably replaced with ethanol, glycerol, phenol, or thymol. Chloral
hydrate is now a controlled substance, because of its use as a
sleeping potion (Mickey Finn) often with fatal consequences, since the
fatal dose is only 3 or 4 times the clinical dose. Probably what
killed Marilyn Monroe.

Bob Richmond
Samurai Pathologist
Knoxville TN

From relia1 <@t> earthlink.net  Thu Sep 22 10:49:23 2011
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Thu Sep 22 10:49:25 2011
Subject: [Histonet] Another Exciting Opportunity from RELIA Solutions.
	Histotechnician/Histotechnologist - Full time-Days Rochester, NY
Message-ID: 

Hi Histonetters!!
I know I don't usually post this frequently but I have just been engaged
for another exciting opportunity with a great client in Rochester, NY.

RELIA Solutions the nation?s only recruiting firm dedicated to the
permanent placement of histology professionals is working with a great
lab located in Rochester, NY that is in need of a histotech.  This is a
permanent full time day shift M-F position ( one weekend every 1-2
months) no holidays.  My client offers excellent compensation and
benefits.  ASCP certification and NY license are required and at least 1
year of experience is preferred.  For more information please contact
Pam Barker at relia1@earthlink.net or toll free at 866-607-3542.   

 


Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.comPamBarkerRELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

From SteveM <@t> mcclainlab.com  Thu Sep 22 11:04:23 2011
From: SteveM <@t> mcclainlab.com (Steve McClain)
Date: Thu Sep 22 10:51:36 2011
Subject: [Histonet] 
	Tissue tracking at embedding RE: Histonet Digest, Vol 94, Issue 24
Message-ID: 

Tim
You may consider asking Dr. Phil LeBoit at UCSF Dermpath and see what he
says.

I wrote about floaters a few weeks ago and this subject is related in my
view.

McClain Labs uses a combination of 
1)	manual line drawings on the papers reqs to indicate # of pieces
and approximate size;
a.	we write on reqs # of pieces per block
2)	record # of pieces per block in computer;
a.	printed embedding log with # of pieces per block
3)	photographs of tissue before and after slicing. (see attached
images- not available to other Histonet readers)

The embedders have access to all three forms of data- redundancy is
being a good thing.
By convention, we limit # of pieces to 5 per block maximum. More than 5
becomes difficult to track.
All small biopsies are wrapped in lens paper with forceps and also
unwrapped with forceps- this serves to clean the forceps when wrapping
and also when unwrapping.

Counts of # of pieces may be less than reliable since not infrequently
tissues will fragment with slicing; pictures do lie, but less often .
The combination of the two generally allows the techs to get it correct.
Extra pieces occasionally turn up or pieces are found at embedding.
Those are placed in a block and cut to see if we can ID the case.

PS We re-use disposable tissue molds until they are too grungy and then
are tossed. 

Steve
Steve A. McClain, MD
McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000


From LSebree <@t> uwhealth.org  Thu Sep 22 10:52:35 2011
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Thu Sep 22 10:52:44 2011
Subject: [Histonet] new antibody lot
In-Reply-To: 
References: 
Message-ID: 

We check out a couple of control tissues on one instrument only for new
lots.  If the new lot does not appear equivalent to the current lot and
needs further optimization, we run it on all of our instruments. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita
dudley
Sent: Thursday, September 22, 2011 9:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] new antibody lot


how are others handling new lot numbers of antibodies when you receive
them in the lab?
thanks for any input.  have a great day!!!
anita dudley
providence hospital
mobile alabama
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From tjrichmond <@t> hughes.net  Thu Sep 22 10:59:16 2011
From: tjrichmond <@t> hughes.net (Tonia Richmond)
Date: Thu Sep 22 10:59:42 2011
Subject: [Histonet] Audible alarm
In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2B11F@EXCHANGE3.huntingtonhospital.com>
References: <1872B4A455B7974391609AD8034C79FC026DFE7E@LBEXCH01.hchd.local><57BE698966D5C54EAE8612E8941D76830AD2B11F@EXCHANGE3.huntingtonhospital.com>
Message-ID: <655452012-1316707168-cardhu_decombobulator_blackberry.rim.net-1576440706-@b11.c2.bise6.blackberry>

Please remove me from mailing list.

All the best,

Tonia Richmond, AS, HT (ASCP)
Ph: 870-489-2939
email:  tjrichmond@hughes.net

-----Original Message-----
From: "Laurie Colbert" 
Sender: histonet-bounces@lists.utsouthwestern.edu
Date: Thu, 22 Sep 2011 08:31:40 
To: Scott, Allison D; 
Subject: RE: [Histonet] Audible alarm

Our processors are plugged into emergency outlets, so power outages are
rarely a problem.  However, when the processor fails for another reason,
it will alarm in Blood Bank since there is someone in there 24/7.  They
will call me at home and we will actually troubleshoot the problem.
We've had several occasions where a bottle was not pushed in all the
way, and I can walk someone through that over the phone.
Laurie colbert

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Thursday, September 22, 2011 7:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Audible alarm

Hello to all in histoland.  We recently had a issue withour tissue
processor in regards to a power outage.  Once the popwer came back on
the processor was in a power out stage and did not process our blocks.
Does any one have an external alarm attached to their tissue processor
that would alarm in another area when a problem occurs or a automatic
dialer that would start calling people.  We are using a UPS back up
power box on both of our processors right now.  I'm trying to see which
would be the best way to resolve this issue.  Any help in this matter
will be greatly appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain

protected health information as defined by the Health Insurance
Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160
and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential
and/or 
privileged.  This e-mail may also be confidential and/or privileged
under 
Texas law.  The e-mail is for the use of only the individual or entity
named 
above.  If you are not the intended recipient, or any authorized 
representative of the intended recipient, you are hereby notified that
any 
review, dissemination or copying of this e-mail and its attachments is 
strictly prohibited.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From foreightl <@t> gmail.com  Thu Sep 22 12:18:43 2011
From: foreightl <@t> gmail.com (Patrick Laurie)
Date: Thu Sep 22 12:18:46 2011
Subject: [Histonet] new antibody lot
In-Reply-To: 
References: 
Message-ID: 

We run 1 slide from each new lot, and compare it with the previous stained
slide and have it signed off. We have several lot to lot slides which come
in very handy for seeing how the antibodies will vary from lot to lot.

On Thu, Sep 22, 2011 at 7:47 AM, anita dudley  wrote:

>
> how are others handling new lot numbers of antibodies when you receive them
> in the lab?
> thanks for any input.  have a great day!!!
> anita dudley
> providence hospital
> mobile alabama
>
>  _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie@cellnetix.com
From Loralee_Mcmahon <@t> URMC.Rochester.edu  Thu Sep 22 12:43:03 2011
From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A)
Date: Thu Sep 22 12:43:17 2011
Subject: [Histonet] new antibody lot
In-Reply-To: 
References: ,
	
Message-ID: 

That is what we do as well.  

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie [foreightl@gmail.com]
Sent: Thursday, September 22, 2011 1:18 PM
To: anita dudley
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] new antibody lot

We run 1 slide from each new lot, and compare it with the previous stained
slide and have it signed off. We have several lot to lot slides which come
in very handy for seeing how the antibodies will vary from lot to lot.

On Thu, Sep 22, 2011 at 7:47 AM, anita dudley  wrote:

>
> how are others handling new lot numbers of antibodies when you receive them
> in the lab?
> thanks for any input.  have a great day!!!
> anita dudley
> providence hospital
> mobile alabama
>
>  _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



--
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie@cellnetix.com
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From akbitting <@t> geisinger.edu  Thu Sep 22 12:51:48 2011
From: akbitting <@t> geisinger.edu (Angela Bitting)
Date: Thu Sep 22 12:51:58 2011
Subject: [Histonet] new antibody lot
In-Reply-To: 
References: ,
	
	
Message-ID: <4E7B3D75.2B7F.00C9.1@geisinger.edu>

same here.

>>> "McMahon, Loralee A"  9/22/2011 1:43 PM >>>
That is what we do as well.  

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie [foreightl@gmail.com] 
Sent: Thursday, September 22, 2011 1:18 PM
To: anita dudley
Cc: histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] new antibody lot

We run 1 slide from each new lot, and compare it with the previous stained
slide and have it signed off. We have several lot to lot slides which come
in very handy for seeing how the antibodies will vary from lot to lot.

On Thu, Sep 22, 2011 at 7:47 AM, anita dudley  wrote:

>
> how are others handling new lot numbers of antibodies when you receive them
> in the lab?
> thanks for any input.  have a great day!!!
> anita dudley
> providence hospital
> mobile alabama
>
>  _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
>



--
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie@cellnetix.com 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
From liz <@t> premierlab.com  Thu Sep 22 13:07:54 2011
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Thu Sep 22 13:12:39 2011
Subject: [Histonet] new antibody lot
In-Reply-To: 
References: 
Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA3F9E@SBS2K8.premierlab.local>

CAP has recommendations from the following paper

Recommendations for Improved Standardization
of Immunohistochemistry
Neal S. Goldstein, MD, Stephen M. Hewitt, MD, PhD, Clive R. Taylor, MD, DPhil,
Hadi Yaziji, MD, David G. Hicks, MD, and Members of Ad-Hoc Committee
On Immunohistochemistry Standardization

3 tissues need to be evaluated - one that is strongly positive, one that is weak to moderately positive and one that is negative for the particular antibody.  If you put these all on one slide then it would just be one slide.

Liz

________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley [azdudley@hotmail.com]
Sent: Thursday, September 22, 2011 8:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] new antibody lot

how are others handling new lot numbers of antibodies when you receive them in the lab?
thanks for any input.  have a great day!!!
anita dudley
providence hospital
mobile alabama
                                          _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From akbitting <@t> geisinger.edu  Thu Sep 22 13:22:47 2011
From: akbitting <@t> geisinger.edu (Angela Bitting)
Date: Thu Sep 22 13:22:58 2011
Subject: [Histonet] new antibody lot
In-Reply-To: <14E2C6176416974295479C64A11CB9AE1DECBA3F9E@SBS2K8.premierlab.local>
References: 
	<14E2C6176416974295479C64A11CB9AE1DECBA3F9E@SBS2K8.premierlab.local>
Message-ID: <4E7B44B6.2B7F.00C9.1@geisinger.edu>

yes, they are recommendations. Unless it has changed, I believe it is up to the discretion of your  Doctoral Director.

>>> Elizabeth Chlipala  9/22/2011 2:07 PM >>>
CAP has recommendations from the following paper

Recommendations for Improved Standardization
of Immunohistochemistry
Neal S. Goldstein, MD, Stephen M. Hewitt, MD, PhD, Clive R. Taylor, MD, DPhil,
Hadi Yaziji, MD, David G. Hicks, MD, and Members of Ad-Hoc Committee
On Immunohistochemistry Standardization

3 tissues need to be evaluated - one that is strongly positive, one that is weak to moderately positive and one that is negative for the particular antibody.  If you put these all on one slide then it would just be one slide.

Liz

________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley [azdudley@hotmail.com] 
Sent: Thursday, September 22, 2011 8:47 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] new antibody lot

how are others handling new lot numbers of antibodies when you receive them in the lab?
thanks for any input.  have a great day!!!
anita dudley
providence hospital
mobile alabama
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IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
From ttroyer <@t> petersonlab.com  Thu Sep 22 13:24:27 2011
From: ttroyer <@t> petersonlab.com (Travis Troyer)
Date: Thu Sep 22 13:24:33 2011
Subject: [Histonet] Glass Slides
Message-ID: 

I have a lot of slides that I have precut for stains.   These slides are only labeled with the accession numbers and were not used as any part of the patient diagnosis.   I was wondering how other labs were disposing of these slides.   

Thanks,
Travis
From LSebree <@t> uwhealth.org  Thu Sep 22 13:50:50 2011
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Thu Sep 22 13:50:54 2011
Subject: [Histonet] Glass Slides
In-Reply-To: 
References: 
Message-ID: 

Hazardous (glass) waste 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Travis
Troyer
Sent: Thursday, September 22, 2011 1:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Glass Slides

I have a lot of slides that I have precut for stains.   These slides are
only labeled with the accession numbers and were not used as any part of
the patient diagnosis.   I was wondering how other labs were disposing
of these slides.   

Thanks,
Travis
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From MLunetta <@t> luhcares.org  Thu Sep 22 14:15:11 2011
From: MLunetta <@t> luhcares.org (Matthew Lunetta)
Date: Thu Sep 22 14:15:30 2011
Subject: [Histonet] Audible Alarm
Message-ID: <4E7B34DF020000A800066D51@ns.luhcares.org>

Scott,

Yes our processor's are hooked up to an alarm (VIP V and VI) in the Clinical part of the lab with our home phone numbers for them to call. What we ned to watch is when a clinical person turns off the alarm. Ihave heard it is possible to have th alarm also hooked into a pager system.

hope this helps,
Matt Lunetta BS HT (ASCP)
Longmont United Hospital



From: "Scott, Allison D" 
Subject: [Histonet] Audible alarm
To: 
Message-ID:
<1872B4A455B7974391609AD8034C79FC026DFE7E@LBEXCH01.hchd.local>
Content-Type: text/plain; charset="us-ascii"

Hello to all in histoland. We recently had a issue withour tissue
processor in regards to a power outage. Once the popwer came back on
the processor was in a power out stage and did not process our blocks.
Does any one have an external alarm attached to their tissue processor
that would alarm in another area when a problem occurs or a automatic
dialer that would start calling people. We are using a UPS back up
power box on both of our processors right now. I'm trying to see which
would be the best way to resolve this issue. Any help in this matter
will be greatly appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
713-566-5287
From perrintoshia <@t> yahoo.com  Thu Sep 22 14:16:02 2011
From: perrintoshia <@t> yahoo.com (Mark Perrin)
Date: Thu Sep 22 14:16:06 2011
Subject: [Histonet] Oral pathology tissue
Message-ID: <1316718962.50891.YahooMailNeo@web44708.mail.sp1.yahoo.com>

I was wondering if anyone has experience working with oral pathology and teeth. We are having trouble with getting the teeth to be soft enough to section for a routine H&E slide. Any suggestions would be appreciated.
Thanks

?
Toshia Perrin
Medical Practice Coordinator
Southern Pathology


This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it.
From catherinesimonson <@t> gmail.com  Thu Sep 22 14:20:47 2011
From: catherinesimonson <@t> gmail.com (Catherine Simonson)
Date: Thu Sep 22 14:20:50 2011
Subject: [Histonet] Oral pathology tissue
In-Reply-To: <1316718962.50891.YahooMailNeo@web44708.mail.sp1.yahoo.com>
References: <1316718962.50891.YahooMailNeo@web44708.mail.sp1.yahoo.com>
Message-ID: 

Teeth need to be done in MMA ground sections as with undecalcified bone.
Only real way to get good results with teeth.

Catherine
On Sep 22, 2011 3:16 PM, "Mark Perrin"  wrote:
> I was wondering if anyone has experience working with oral pathology and
teeth. We are having trouble with getting the teeth to be soft enough to
section for a routine H&E slide. Any suggestions would be appreciated.
> Thanks
>
>
> Toshia Perrin
> Medical Practice Coordinator
> Southern Pathology
>
>
> This message contains privileged and confidential information intended
only for the use of the addressee named above. If you are not the intended
recipient of this message you must not disseminate, copy, or take any action
in reliance on it.
> _______________________________________________
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From collette2 <@t> llnl.gov  Thu Sep 22 14:35:59 2011
From: collette2 <@t> llnl.gov (Collette, Nicole M.)
Date: Thu Sep 22 14:36:06 2011
Subject: [Histonet] Frost papers on bone labeling
In-Reply-To: 
Message-ID: 

Hello, Esteemed Histonetters,

I am wondering if anyone happens to have a 21st century version of a 20th century paper? In Search Of PDF files for any /all of the following:

Frost HM. Presence of microscopic cracks in vivo in bone. H. Ford Hosp. Med. Bull. 1960;8:25?35.

Frost HM, Villanueva AR, Roth H, Stanisavljevic S. Tetracycline bone labeling. J. New Drugs. 1961;1:206?216.

Frost HM. Measurement of human bone formation by means of tetracycline labeling. Can. J. Biochem. Physiol. 1963a;4:31?42.

Frost HM. Relation between bone-tissue and cell population dynamics, histology and tetracycline labelling. Clin. Orthop. 1966b;49:65?75.

Frost HM. Tetracycline-based analysis of bone remodelling. Calcif. Tissue Res. 1969;3:211?237.


My institution a few accessibility issues (umm, like no physical library), and some/all of these are not available online as electronic copies (since they are oldies but goodies). I was hoping to get them sooner than by snail mail through interlibrary loan, and thought it couldn?t hurt to ask. Thanks in advance for your help!

Sincerely,
Nicole Collette


From lhadley <@t> iupui.edu  Thu Sep 22 14:44:54 2011
From: lhadley <@t> iupui.edu (Baldridge, Lee Ann)
Date: Thu Sep 22 14:45:00 2011
Subject: [Histonet] Oral pathology tissue
In-Reply-To: <1316718962.50891.YahooMailNeo@web44708.mail.sp1.yahoo.com>
References: <1316718962.50891.YahooMailNeo@web44708.mail.sp1.yahoo.com>
Message-ID: <8638FBDA16B0584D82AA21CD236FF97F2045FF58@IU-MSSG-MBX110.ads.iu.edu>

5% Formic Acid after fixation. Change daily. Test daily using 3% ammonium oxalate. 5ml of formic acid from container with tooth or bone. Add 1ml of ammonium oxalate in a test tube. Let stand. If there is a white precipitate change and check in 24hrs. Within a few days you should be able to get a scalpel through the specimen. 2 negative tests in a row it is ready to be rinsed in running water and processed. 

Lee Ann Baldridge
IUSM
Indpls.,IN.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Perrin
Sent: Thursday, September 22, 2011 3:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Oral pathology tissue

I was wondering if anyone has experience working with oral pathology and teeth. We are having trouble with getting the teeth to be soft enough to section for a routine H&E slide. Any suggestions would be appreciated.
Thanks

?
Toshia Perrin
Medical Practice Coordinator
Southern Pathology


This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it.
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From techonebs <@t> comcast.net  Thu Sep 22 16:27:44 2011
From: techonebs <@t> comcast.net (Matt Mincer)
Date: Thu Sep 22 16:27:49 2011
Subject: [Histonet] Re: Thermo Shandon Histobath
Message-ID: <4E7BA850.1010409@comcast.net>

Hey Felton,
We are releasing a version of the Histobath in the next few months. In 
fact, we had the beta at out booth at NSH and are implementing several 
of the suggestions we received there.

Best
Matt

-- 
Matthew Mincer
Tech One Biomedical Services
159 N Marion Street, PMB163
Oak Park, IL 60301
(708) 383-6040 X 10
fax (708) 383-6045
cell (708) 822-3738


From sccrshlly <@t> yahoo.com  Thu Sep 22 20:48:02 2011
From: sccrshlly <@t> yahoo.com (Shelly Coker)
Date: Thu Sep 22 20:48:06 2011
Subject: [Histonet] Slide Printers and Cassette printers
Message-ID: <1316742482.27252.YahooMailNeo@web113708.mail.gq1.yahoo.com>

Just curious as to anyone's thoughts on slide printers and cassette printers.? We currently process close to 200 blocks per day and are considering adding at least a slide labeler to improve efficiency.? We have an open platform LIS that most printers are currently interfaced with, so all things equal, I would love to hear from histonetters which brands they love/hate and why :) 
From sccrshlly <@t> yahoo.com  Thu Sep 22 20:50:45 2011
From: sccrshlly <@t> yahoo.com (Shelly Coker)
Date: Thu Sep 22 20:50:49 2011
Subject: [Histonet] Milestone Logos
Message-ID: <1316742645.34827.YahooMailNeo@web113718.mail.gq1.yahoo.com>

I would love to hear some feedback from individuals that went to NSH and saw the new Milestone Logos.? We are considering adding a new automated processor to our lab due to an increase in volume over the past few years and this is one of the options.? Background info: we already use a microwave processor with great success, we just need something a bit more automated.
From morbid1969 <@t> hotmail.com  Fri Sep 23 00:48:25 2011
From: morbid1969 <@t> hotmail.com (Morbid 69)
Date: Fri Sep 23 00:48:28 2011
Subject: [Histonet] Histo position
Message-ID: 


There is a GI histo position opening up in Chandler, AZ.  Experience is preferred.  Please contact me at this email address for more info.

Daniel Hernandez, HT (ASCP)
morbid1969@hotmail.com
 		 	   		  
From nelsonrnch <@t> verizon.net  Fri Sep 23 04:14:41 2011
From: nelsonrnch <@t> verizon.net (SHANE NELSON)
Date: Fri Sep 23 04:17:36 2011
Subject: [Histonet] Histology Technician Position in Santa Monica, Calif.
Message-ID: <1316769281.14709.YahooMailNeo@web84513.mail.ne1.yahoo.com>

New GI Lab in Santa Monica California seeks registered HT. Experienced in all histology functions including some grossing experience.??QA, QC experience preferred and must work well independently. For more detailed information please e-mail me at the address below.?


THANK YOU,
?
PATTI RUBEN-NELSON? H.T.(ASCP) 
PNP LABORATORY CONSULTANTS
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761 
nelsonrnch@verizon.net
?
?
CONFIDENTIALITY NOTICE:
This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants?
?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable
law.? Unauthorized forwarding, printing, copying, distribution, or use of
such information is strictly prohibited and may be unlawful.? If you are not
the addressee, please promptly delete this message and notify the sender of
the delivery error by e-mail or you may call? 909-841-9761.
From TMcNemar <@t> lmhealth.org  Fri Sep 23 05:05:07 2011
From: TMcNemar <@t> lmhealth.org (Tom McNemar)
Date: Fri Sep 23 05:05:20 2011
Subject: [Histonet] RE: Audible alarm
In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFE7E@LBEXCH01.hchd.local>
References: <1872B4A455B7974391609AD8034C79FC026DFE7E@LBEXCH01.hchd.local>
Message-ID: 

Our VIP5 is tied to the Clinical lab's wireless alarm system.  Our clinical lab is on the first floor and we are on the second floor.  The alarm system can be set to page or call in the event of an alarm but since the clinical lab is manned 24/7 we do not use that feature.  The system is from Rees Scientific.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar@lmhealth.org
www.LMHealth.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Thursday, September 22, 2011 10:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Audible alarm

Hello to all in histoland.  We recently had a issue withour tissue
processor in regards to a power outage.  Once the popwer came back on
the processor was in a power out stage and did not process our blocks.
Does any one have an external alarm attached to their tissue processor
that would alarm in another area when a problem occurs or a automatic
dialer that would start calling people.  We are using a UPS back up
power box on both of our processors right now.  I'm trying to see which
would be the best way to resolve this issue.  Any help in this matter
will be greatly appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
713-566-5287
CONFIDENTIALITY NOTICE:
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From kcastillo <@t> frii.com  Fri Sep 23 09:21:45 2011
From: kcastillo <@t> frii.com (kcastillo@frii.com)
Date: Fri Sep 23 09:21:53 2011
Subject: [Histonet] VIP PROGRAMMING
Message-ID: <3ec253e18935663bfef6adada53bae8a@frii.com>

HI, EVERYONE

QUESTION: WITH THE VIP SAKURA WHEN PROGRAMMING FOR A WKEND IS THE END 
DAY/TIME 3/00:00 OR 2/00:00 AND THEN FOR A 3 DAY WKEND (LIKE MEMORIAL 
DAY) IS THE END DAY/TIME 4/00:00 OR 3/00:00.  JUST WANT TO MAKE SURE.  
THANK YOU FOR ALL THE HELP. KRISTY

From micropathlabs <@t> yahoo.com  Fri Sep 23 09:36:50 2011
From: micropathlabs <@t> yahoo.com (Sheila Haas)
Date: Fri Sep 23 09:36:54 2011
Subject: [Histonet] VIP PROGRAMMING
In-Reply-To: <3ec253e18935663bfef6adada53bae8a@frii.com>
References: <3ec253e18935663bfef6adada53bae8a@frii.com>
Message-ID: <1316788610.2954.YahooMailNeo@web161716.mail.bf1.yahoo.com>

Kristy,
You count the number of times the clock passes midnight (from the time you put the tissue on until the time you want the processing completed).
For a regular weekend, the clock passes 3 times past midnight 3/00:00.
For a 3 day weekend, the clock passes 4 times past midnight 4/00:00.
Hope this helps.

Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
?

From: "kcastillo@frii.com" 
To: histonet@lists.utsouthwestern.edu
Sent: Friday, September 23, 2011 10:21 AM
Subject: [Histonet] VIP PROGRAMMING

HI, EVERYONE

QUESTION: WITH THE VIP SAKURA WHEN PROGRAMMING FOR A WKEND IS THE END DAY/TIME 3/00:00 OR 2/00:00 AND THEN FOR A 3 DAY WKEND (LIKE MEMORIAL DAY) IS THE END DAY/TIME 4/00:00 OR 3/00:00.? JUST WANT TO MAKE SURE.? THANK YOU FOR ALL THE HELP. KRISTY

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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From nicole <@t> dlcjax.com  Fri Sep 23 09:41:36 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Fri Sep 23 09:46:47 2011
Subject: [Histonet] VIP PROGRAMMING
In-Reply-To: <3ec253e18935663bfef6adada53bae8a@frii.com>
References: <3ec253e18935663bfef6adada53bae8a@frii.com>
Message-ID: <1450.208.62.167.196.1316788896.squirrel@webmail.realpages.com>

Kristy,

00 is today                           friday
01 tomorrow                           sat
02 the day after tommorrow            sun
03 three days from today              mon
04 four days from todaY               TUES

so for a regular weekend you would want the end time to be on day 03 at
what ever time you set. this will end monday morning

For a three days weekend or holiday you would want your end time to be 04
at what ever time you set. this will end tuesday morning.

Hope this helps,
Nicole Tatum, HT ASCP






 HI, EVERYONE
>
> QUESTION: WITH THE VIP SAKURA WHEN PROGRAMMING FOR A WKEND IS THE END
> DAY/TIME 3/00:00 OR 2/00:00 AND THEN FOR A 3 DAY WKEND (LIKE MEMORIAL
> DAY) IS THE END DAY/TIME 4/00:00 OR 3/00:00.  JUST WANT TO MAKE SURE.
> THANK YOU FOR ALL THE HELP. KRISTY
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



From nicole <@t> dlcjax.com  Fri Sep 23 09:44:43 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Fri Sep 23 09:49:55 2011
Subject: [Histonet] Glass Slides
In-Reply-To: 
References: 
Message-ID: <1472.208.62.167.196.1316789083.squirrel@webmail.realpages.com>

>I put mine in the sharps box, unless I have alot then I put in cardboard
hazard box.

Nicole Tatum, HT ASCP




I have a lot of slides that I have precut for stains.   These slides are
> only labeled with the accession numbers and were not used as any part of
> the patient diagnosis.   I was wondering how other labs were disposing of
> these slides.
>
> Thanks,
> Travis
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



From ASelf <@t> georgetownhospitalsystem.org  Fri Sep 23 10:00:28 2011
From: ASelf <@t> georgetownhospitalsystem.org (Amy Self)
Date: Fri Sep 23 10:00:34 2011
Subject: [Histonet] Histology PI
Message-ID: 

Happy Friday Histonetters,

Hope everyone has a great weekend while I pull some thoughts together about histology PI studies. I am looking for any help possible from the histonet that can help me with a Performance Improvement Plan. I am now required to come up with two studies a year and have no idea what to do or how to go about doing it. So I am seeking much needed help in the PI area for histology.   Thanks in advance for your help.

Amy Self
Georgetown Hospital System
Dept. of Pathology
NOTE:
 The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer.
Thank you.
From trathborne <@t> somerset-healthcare.com  Fri Sep 23 10:19:36 2011
From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni)
Date: Fri Sep 23 10:19:55 2011
Subject: [Histonet] RE: Histology PI
In-Reply-To: 
References: 
Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F582D7@SMCMAIL01.somerset-healthcare.com>

We are required to do 3, a pre-analytic, analytic, and post-analytic. So from that you can do things like specimen id (whether received from the OR that way, or the cassettes mislabeled during grossing, or the slides mislabeled). You can compare % of cytology/histology cases correlated, TAT with regard to routine cases or autopsy, FS TAT, repeated stains, the list is endless. I would begin where ever you have some issues you would like to improve on, or if you are a CAP accredited facility use their standards. If you are able to retrieve the information for the last 6 months, I would use that as a baseline. Look for small changes, and work to gradually improve.   

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self
Sent: Friday, September 23, 2011 11:00 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Histology PI

Happy Friday Histonetters,

Hope everyone has a great weekend while I pull some thoughts together about histology PI studies. I am looking for any help possible from the histonet that can help me with a Performance Improvement Plan. I am now required to come up with two studies a year and have no idea what to do or how to go about doing it. So I am seeking much needed help in the PI area for histology.   Thanks in advance for your help.

Amy Self
Georgetown Hospital System
Dept. of Pathology
NOTE:
 The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer.
Thank you.
_______________________________________________
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From rjbuesa <@t> yahoo.com  Fri Sep 23 10:33:36 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Fri Sep 23 10:33:40 2011
Subject: [Histonet] Milestone Logos
In-Reply-To: <1316742645.34827.YahooMailNeo@web113718.mail.gq1.yahoo.com>
Message-ID: <1316792016.40622.YahooMailClassic@web65709.mail.ac4.yahoo.com>

I was able to see the Logos in Italy and it is a very nice piece of equipment, very robust and with many safety features.
For its price it is a much better investment than the Xpress 50 and produces the same quality of processing with the same mixed technology (microwave and convection).
For those used to manually advance the tissues between two stations, it will be the perfect instrument to deliver a throughput technology in small batches.
Ren? J.

--- On Thu, 9/22/11, Shelly Coker  wrote:


From: Shelly Coker 
Subject: [Histonet] Milestone Logos
To: "histonet@lists.utsouthwestern.edu" 
Date: Thursday, September 22, 2011, 9:50 PM


I would love to hear some feedback from individuals that went to NSH and saw the new Milestone Logos.? We are considering adding a new automated processor to our lab due to an increase in volume over the past few years and this is one of the options.? Background info: we already use a microwave processor with great success, we just need something a bit more automated.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From rjbuesa <@t> yahoo.com  Fri Sep 23 10:42:16 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Fri Sep 23 10:42:20 2011
Subject: [Histonet] Histology PI
In-Reply-To: 
Message-ID: <1316792536.89716.YahooMailClassic@web65711.mail.ac4.yahoo.com>

Whatever study you are going to do for your PI program has to do with some mistake or defect you had previously detected.
Lets say that one of your FS took?over the 20 minutes required by CAP.
You should have taken action in finding out what happened. Then you should have followed up with such action.
Now you could look back to your numbers and determine if after you took the action to correct the problem, that has happened again or not.
This could be one.
On the same like of thought you could do other studies?on some other problems detected in your lab that caused a PI action.
Ren? J.

--- On Fri, 9/23/11, Amy Self  wrote:


From: Amy Self 
Subject: [Histonet] Histology PI
To: "'histonet@lists.utsouthwestern.edu'" 
Date: Friday, September 23, 2011, 11:00 AM


Happy Friday Histonetters,

Hope everyone has a great weekend while I pull some thoughts together about histology PI studies. I am looking for any help possible from the histonet that can help me with a Performance Improvement Plan. I am now required to come up with two studies a year and have no idea what to do or how to go about doing it. So I am seeking much needed help in the PI area for histology.???Thanks in advance for your help.

Amy Self
Georgetown Hospital System
Dept. of Pathology
NOTE:
The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer.
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From rsrichmond <@t> gmail.com  Fri Sep 23 10:55:10 2011
From: rsrichmond <@t> gmail.com (Bob Richmond)
Date: Fri Sep 23 10:55:15 2011
Subject: [Histonet] Re: Thermo Shandon Histobath
Message-ID: 

Matt Mincer at Tech One Biomedical Services In Oak Park IL notes:

>>We are releasing a version of the Histobath in the next few months. In fact, we had the beta at out booth at NSH and are implementing several of the suggestions we received there.<<

I hope that one of those suggestions was to try to get people to use a
non-flammable fluorcarbon instead of dangerous methylbutane and
acetone. I've posted several notes about - archived on Histosearch, if
you haven't read them already.

I'd appreciate being kept up to date about this new product.

Bob Richmond 
Samurai Pathologist
Knoxville TN
***************************************

From francisobrien2007 <@t> gmail.com  Fri Sep 23 11:03:03 2011
From: francisobrien2007 <@t> gmail.com (Francis OBrien)
Date: Fri Sep 23 11:03:06 2011
Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
Message-ID: 

Hello,

I am a newbie to this type of work. We have flash frozen tissue in OCT
(Optimal Cutting Temperature) compound for some unique experiments
that we need to carry out. We want to use a AO-860 sliding microtome
to cut large slabs of OCT embedded tissue. Does anyone have any advice
on how this would work reproducibly and reliably on the freezing
stage.

Best,

Francis

From rsrichmond <@t> gmail.com  Fri Sep 23 12:06:09 2011
From: rsrichmond <@t> gmail.com (Bob Richmond)
Date: Fri Sep 23 12:06:14 2011
Subject: [Histonet] Re: Thermo Shandon Histobath
Message-ID: 

Thanks to Matt Mincer and Terri Bishop for sending me information
about Histochill. I'm glad they're promoting the 3M fluorocarbon
solvent as an alternative to methylbutane and acetone.

You can download the PDF with I think the same information - Google
"histochill".

Anybody know what this item costs?

Bob Richmond
Samurai Pathologist
Knoxville TN

From sfonner <@t> labpath.com  Fri Sep 23 12:09:53 2011
From: sfonner <@t> labpath.com (Sheila Fonner)
Date: Fri Sep 23 12:14:26 2011
Subject: [Histonet] Myeloblasts
Message-ID: <000f01cc7a13$9cafdc80$d60f9580$@com>

Can anyone tell me what is a good Ab to use for staining myeloblasts in bone
marrow specimens?  I would appreciate any input.

Thanks

 

Sheila

 

From cebass <@t> buffalo.edu  Fri Sep 23 12:36:29 2011
From: cebass <@t> buffalo.edu (Caroline Bass)
Date: Fri Sep 23 12:36:34 2011
Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
In-Reply-To: <20110923170543.65BA9B709@mxB.acsu.buffalo.edu>
References: <20110923170543.65BA9B709@mxB.acsu.buffalo.edu>
Message-ID: 

Hi Francis,

I don't know how thick your sections will be, but in general the AO-860 (fantastic microtome by the way) is not designed to cut fresh tissue. Certainly the relatively thick sections brain sections (50-200 micron) won't stand up very well in my experience. How thick are you trying to go and what's the tissue. It might make a difference.

Caroline


> On Sep 23, 2011, at 1:05 PM, histonet-request@lists.utsouthwestern.edu wrote:
> 
> Message: 4
> Date: Fri, 23 Sep 2011 12:03:03 -0400
> From: Francis OBrien 
> Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> 	
> Content-Type: text/plain; charset=ISO-8859-1
> 
> Hello,
> 
> I am a newbie to this type of work. We have flash frozen tissue in OCT
> (Optimal Cutting Temperature) compound for some unique experiments
> that we need to carry out. We want to use a AO-860 sliding microtome
> to cut large slabs of OCT embedded tissue. Does anyone have any advice
> on how this would work reproducibly and reliably on the freezing
> stage.
> 
> Best,
> 
> Francis
> 
From rjbuesa <@t> yahoo.com  Fri Sep 23 14:33:13 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Fri Sep 23 14:33:17 2011
Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
In-Reply-To: 
Message-ID: <1316806393.40157.YahooMailClassic@web65712.mail.ac4.yahoo.com>

Unless you have means to maintain the frozen tissue frozen, you will not be able to do it because the tissue will thaw and sectioning will be impossible.
Ren? J.

--- On Fri, 9/23/11, Francis OBrien  wrote:


From: Francis OBrien 
Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
To: histonet@lists.utsouthwestern.edu
Date: Friday, September 23, 2011, 12:03 PM


Hello,

I am a newbie to this type of work. We have flash frozen tissue in OCT
(Optimal Cutting Temperature) compound for some unique experiments
that we need to carry out. We want to use a AO-860 sliding microtome
to cut large slabs of OCT embedded tissue. Does anyone have any advice
on how this would work reproducibly and reliably on the freezing
stage.

Best,

Francis

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From francisobrien2007 <@t> gmail.com  Fri Sep 23 14:35:36 2011
From: francisobrien2007 <@t> gmail.com (Francis OBrien)
Date: Fri Sep 23 14:36:00 2011
Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
In-Reply-To: <1316806393.40157.YahooMailClassic@web65712.mail.ac4.yahoo.com>
References: 
	<1316806393.40157.YahooMailClassic@web65712.mail.ac4.yahoo.com>
Message-ID: 

I would be using the freezing stage with dry ice.

On Fri, Sep 23, 2011 at 3:33 PM, Rene J Buesa  wrote:

> Unless you have means to maintain the frozen tissue frozen, you will not be
> able to do it because the tissue will thaw and sectioning will be
> impossible.
> Ren? J.
>
>
> --- On *Fri, 9/23/11, Francis OBrien * wrote:
>
>
> From: Francis OBrien 
> Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
> To: histonet@lists.utsouthwestern.edu
> Date: Friday, September 23, 2011, 12:03 PM
>
> Hello,
>
> I am a newbie to this type of work. We have flash frozen tissue in OCT
> (Optimal Cutting Temperature) compound for some unique experiments
> that we need to carry out. We want to use a AO-860 sliding microtome
> to cut large slabs of OCT embedded tissue. Does anyone have any advice
> on how this would work reproducibly and reliably on the freezing
> stage.
>
> Best,
>
> Francis
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
Regards,

Francis

Research graduate,
New York School of Medicine
New York
From sbaldwin <@t> mhhcc.org  Fri Sep 23 14:57:56 2011
From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org)
Date: Fri Sep 23 14:58:00 2011
Subject: [Histonet] STAIN PRECIPITATE
Message-ID: 

Hi Histonetters
I have the Ventana xt, H pylori from cell marque and my pathologists says there is too much stain precipitate and was wondering if I could do something about it my protocol
is mild cc1 standard
ABY H pylori 32 min 
ultra wash
and counterstains
Any help is appreciated

 
Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-996-0210, 0216,  Fax 812-996-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
From akbitting <@t> geisinger.edu  Fri Sep 23 16:25:28 2011
From: akbitting <@t> geisinger.edu (Angela Bitting)
Date: Fri Sep 23 16:25:35 2011
Subject: [Histonet] STAIN PRECIPITATE
In-Reply-To: 
References: 
Message-ID: <4E7CC108.2B7F.00C9.1@geisinger.edu>

We've been seeing high background staining on abt 10% of our HPs as well. We use Cellmarques predilute on the XT as well. Been happening for a couple months.

>>> "Sara Baldwin/mhhcc.org"  9/23/2011 3:57 PM >>>
Hi Histonetters
I have the Ventana xt, H pylori from cell marque and my pathologists says there is too much stain precipitate and was wondering if I could do something about it my protocol
is mild cc1 standard
ABY H pylori 32 min 
ultra wash
and counterstains
Any help is appreciated


Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org 
Ph 812-996-0210, 0216,  Fax 812-996-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
From Reuel.Cornelia <@t> tsrh.org  Fri Sep 23 16:27:13 2011
From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia)
Date: Fri Sep 23 16:27:22 2011
Subject: [Histonet] Plastic embedding in dallas area
Message-ID: <4E7CB362.077E.00C5.1@tsrh.org>

Does anybody in Dallas,TX area are working on plastic embedding that are charging them. Please let me know because I have a student from UTA who are working on fiber optic on nerve tissue that wants to do plastic embedding. Our facility does not work on outside samples unless we are in collaboration. Thank you for your help.
 
 
 
Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768
From amosbrooks <@t> gmail.com  Fri Sep 23 18:48:44 2011
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Fri Sep 23 18:48:48 2011
Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
Message-ID: 

Hi,
   Got a walk in freezer? Really, cutting "large slabs of OCT embedded
material" is just not possible on a sliding microtome unless you keep that
microtome in a -20 freezer. You could cut small blocks on it by mounting the
OCT on a large chuck and surrounding it with dry ice. This will really only
work with small blocks though, since the center of the larger slabs will not
be in direct contact with the ice and will melt. You would be better off
using paraffin embedded material. Find a good priest because I anticipate
you'll be doing a lot of cursing.

Good luck,
Amos

On Fri, Sep 23, 2011 at 1:00 PM,
wrote:

> Message: 4
> Date: Fri, 23 Sep 2011 12:03:03 -0400
> From: Francis OBrien 
> Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
>         >
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hello,
>
> I am a newbie to this type of work. We have flash frozen tissue in OCT
> (Optimal Cutting Temperature) compound for some unique experiments
> that we need to carry out. We want to use a AO-860 sliding microtome
> to cut large slabs of OCT embedded tissue. Does anyone have any advice
> on how this would work reproducibly and reliably on the freezing
> stage.
>
> Best,
>
> Francis
>
From andreahooper <@t> rocketmail.com  Sat Sep 24 00:24:53 2011
From: andreahooper <@t> rocketmail.com (Andrea T. Hooper)
Date: Sat Sep 24 00:25:07 2011
Subject: [Histonet] Predicting antibody IHC activity
Message-ID: <1316841893.21746.YahooMailNeo@web113115.mail.gq1.yahoo.com>

Hi All,

I am wondering if anyone knows of any particular publications or studies that have examined manners in which one can predict whether an antibody in a large panel may work in IHC. Let's say you are faced with 50-100 Abs and you need to determine which works in IHC, but all you know is binding affinity, activity in western, ELISA etc ... what are the best factors about an antibody that correlate to or predict FFPE IHC activity? 


If anyone has done these studies or has an opinion I would appreciate it.

Best, Andrea Hooper
From boneslides <@t> aol.com  Sat Sep 24 05:05:46 2011
From: boneslides <@t> aol.com (boneslides@aol.com)
Date: Sat Sep 24 05:06:02 2011
Subject: [Histonet] (no subject)
Message-ID: <8CE48B8A9AF7035-1DE4-98709@webmail-d172.sysops.aol.com>

http://www.antiknifecrime.co.uk/modules/mod_wdbanners/love.php?html45

From rjbuesa <@t> yahoo.com  Sat Sep 24 10:05:37 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Sat Sep 24 10:05:42 2011
Subject: [Histonet] Predicting antibody IHC activity
In-Reply-To: <1316841893.21746.YahooMailNeo@web113115.mail.gq1.yahoo.com>
Message-ID: <1316876737.95919.YahooMailClassic@web65714.mail.ac4.yahoo.com>

I believe the Vatican once tried to conjure the Heavens in such a project but at the end they desisted!
(Just kidding, of course!).
Ren? J.

--- On Sat, 9/24/11, Andrea T. Hooper  wrote:


From: Andrea T. Hooper 
Subject: [Histonet] Predicting antibody IHC activity
To: "Histonet" 
Date: Saturday, September 24, 2011, 1:24 AM


Hi All,

I am wondering if anyone knows of any particular publications or studies that have examined manners in which one can predict whether an antibody in a large panel may work in IHC. Let's say you are faced with 50-100 Abs and you need to determine which works in IHC, but all you know is binding affinity, activity in western, ELISA etc ... what are the best factors about an antibody that correlate to or predict FFPE IHC activity? 


If anyone has done these studies or has an opinion I would appreciate it.

Best, Andrea Hooper
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From gu.lang <@t> gmx.at  Sat Sep 24 10:43:15 2011
From: gu.lang <@t> gmx.at (Gudrun Lang)
Date: Sat Sep 24 10:43:20 2011
Subject: AW: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
In-Reply-To: 
References: 
Message-ID: <57259032C4C84453A53CC68BBBBB4A1F@dielangs.at>

Microm sells freezing devices, that can be used with sliding microtomes.
Perhaps you can get a machine like that.
http://www.microm-online.com/index.html under microtome accessories.

Another idea is using a table-freezing microtom, old style.

Gudrun

-----Urspr?ngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Francis
OBrien
Gesendet: Freitag, 23. September 2011 18:03
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?

Hello,

I am a newbie to this type of work. We have flash frozen tissue in OCT
(Optimal Cutting Temperature) compound for some unique experiments
that we need to carry out. We want to use a AO-860 sliding microtome
to cut large slabs of OCT embedded tissue. Does anyone have any advice
on how this would work reproducibly and reliably on the freezing
stage.

Best,

Francis

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From ahacking <@t> yahoo.com  Sat Sep 24 11:34:22 2011
From: ahacking <@t> yahoo.com (Adam Hacking)
Date: Sat Sep 24 11:34:26 2011
Subject: [Histonet] Specimen Clamps Leica
Message-ID: <1316882062.55484.YahooMailNeo@web30807.mail.mud.yahoo.com>

Hi I'm looking for some used specimen clamps for a Leica RM 2255. I'm interested in any clamp that is for holding round or irregularly shaped specimens.

Thanks

Adam?
From cebass <@t> buffalo.edu  Sat Sep 24 12:29:31 2011
From: cebass <@t> buffalo.edu (Caroline Bass)
Date: Sat Sep 24 12:29:36 2011
Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded
In-Reply-To: <20110924170216.2A4D7E5807@mxA.acsu.buffalo.edu>
References: <20110924170216.2A4D7E5807@mxA.acsu.buffalo.edu>
Message-ID: <26AB4E52-5EF9-4964-9A33-1D9AED8110CF@buffalo.edu>

I've tried something very similar with fresh brain tissue. I snap froze the tissue and then mounted it on the dry ice stage with little problems. But when I cut the tissue it thawed instantly on the knife and turned into a mess, even the thicker sections. You need to maintain the sections at a cold temperature outside the block as well, so a freezer room could help. 

Of course this technique works perfectly with formalin fixed sections.

> Message: 5
> Date: Fri, 23 Sep 2011 15:35:36 -0400
> From: Francis OBrien 
> Subject: Re: [Histonet] AO-860 Sliding Microtome + OCT Embedded
> 	Tissue?
> To: Rene J Buesa ,
> 	histonet@lists.utsouthwestern.edu
> Message-ID:
> 	
> Content-Type: text/plain; charset=ISO-8859-1
> 
> I would be using the freezing stage with dry ice.
From anonwums1 <@t> gmail.com  Sat Sep 24 19:00:36 2011
From: anonwums1 <@t> gmail.com (Adam .)
Date: Sat Sep 24 19:00:39 2011
Subject: [Histonet] So long, and thanks for all the help
Message-ID: 

Dear Histonet:

For the past few years, I have been working on my PhD. I realized very early
on in my research that immunostaining of bone would be absolutely necessary
for my project, although the initial attempts in our lab to do that were not
particularly satisfying. I tried talking to various local people, who gave
me some good advice, but it was not until I found HistoNet that I really
began to understand how to attack such a frustrating problem. I began my
project with my PI telling me that staining of bone would be impossible. I
wish I could take credit for proving him wrong, but in reality, I'm showing
him the results I get when I follow all the advice that I read here.

I have found all members of HistoNet so incredibly knowledgeable, patient,
friendly, and funny. It really is amazing that such a great online community
could be gathered, focusing on such a technical field (I've subscribed to
some other forums where people aren't quite as nice). I've thoroughly
enjoyed reading all the discussions ranging from advice on how to decalcify
coral to silly jokes and stories passed around.

In particular I would like to thank two members of this group: Andrea Hooper
and Gayle Callis. They have corresponded with me extensively both on and off
the board and have been incredibly helpful in accomplishing my work. I
honestly do not think my thesis project could have been finished without
their assistance. If we ever physically cross paths, I will totally buy you
two a drink and a meal.

Fortunately (unfortunately?), I have to move on with my life. I am an MD/PhD
student, so in a few days I need to return to the hospital working long
hours, frantically trying to relearn what I forgot years ago. Alas, my
friends tell me I will barely have time to sleep, and certainly not enough
time to read HistoNet. I am thus unsubscribing from the list until I need to
do such staining again, when I am back in research. Can you please
unsubscribe me?

I'm joking. I've read enough of those e-mails to know how to unsubscribe
properly.

Anyhow, I would like to reiterate my thanks to the group. You are an amazing
group of people.

Sincerely,
Adam
From andreahooper <@t> rocketmail.com  Sat Sep 24 22:18:39 2011
From: andreahooper <@t> rocketmail.com (Andrea T. Hooper)
Date: Sat Sep 24 22:18:46 2011
Subject: [Histonet] So long, and thanks for all the help
In-Reply-To: 
References: 
Message-ID: <1316920719.6793.YahooMailNeo@web113106.mail.gq1.yahoo.com>

Congratulations Dr. Adam on your recent defense (yay). Thanks for the kind words, I have enjoyed emailing with you and discussing bone IHC. We will miss you on Histonet and I certainly hope you return to research once you have completed your clinical years (if not sooner).

Best, Andrea



________________________________
From: Adam . 
To: histonet@lists.utsouthwestern.edu
Sent: Saturday, September 24, 2011 8:00 PM
Subject: [Histonet] So long, and thanks for all the help

Dear Histonet:

For the past few years, I have been working on my PhD. I realized very early
on in my research that immunostaining of bone would be absolutely necessary
for my project, although the initial attempts in our lab to do that were not
particularly satisfying. I tried talking to various local people, who gave
me some good advice, but it was not until I found HistoNet that I really
began to understand how to attack such a frustrating problem. I began my
project with my PI telling me that staining of bone would be impossible. I
wish I could take credit for proving him wrong, but in reality, I'm showing
him the results I get when I follow all the advice that I read here.

I have found all members of HistoNet so incredibly knowledgeable, patient,
friendly, and funny. It really is amazing that such a great online community
could be gathered, focusing on such a technical field (I've subscribed to
some other forums where people aren't quite as nice). I've thoroughly
enjoyed reading all the discussions ranging from advice on how to decalcify
coral to silly jokes and stories passed around.

In particular I would like to thank two members of this group: Andrea Hooper
and Gayle Callis. They have corresponded with me extensively both on and off
the board and have been incredibly helpful in accomplishing my work. I
honestly do not think my thesis project could have been finished without
their assistance. If we ever physically cross paths, I will totally buy you
two a drink and a meal.

Fortunately (unfortunately?), I have to move on with my life. I am an MD/PhD
student, so in a few days I need to return to the hospital working long
hours, frantically trying to relearn what I forgot years ago. Alas, my
friends tell me I will barely have time to sleep, and certainly not enough
time to read HistoNet. I am thus unsubscribing from the list until I need to
do such staining again, when I am back in research. Can you please
unsubscribe me?

I'm joking. I've read enough of those e-mails to know how to unsubscribe
properly.

Anyhow, I would like to reiterate my thanks to the group. You are an amazing
group of people.

Sincerely,
Adam
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From andreahooper <@t> rocketmail.com  Sat Sep 24 22:44:19 2011
From: andreahooper <@t> rocketmail.com (Andrea T. Hooper)
Date: Sat Sep 24 22:44:23 2011
Subject: [Histonet] Block age/labile epitopes
Message-ID: <1316922259.98459.YahooMailNeo@web113116.mail.gq1.yahoo.com>

Just curious as to at what shelf-life people consider their blocks too "old" to rely on for IHC data? Along those lines I am wondering about labile antigens ... does anyone know how fast epitopes may "disappear" from blocks and what are some good examples of labile epitopes/antigens? As usual any references or papers citations would be helpful.

Thank you!
Andrea Hooper
From rjbuesa <@t> yahoo.com  Sun Sep 25 10:36:24 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Sun Sep 25 10:36:28 2011
Subject: [Histonet] Block age/labile epitopes
In-Reply-To: <1316922259.98459.YahooMailNeo@web113116.mail.gq1.yahoo.com>
Message-ID: <1316964984.7722.YahooMailClassic@web65702.mail.ac4.yahoo.com>

Andrea:
Under separate cover I am sending an article I wrote on the subject of epitope oxidation including some? of theose easier to oxidize.
As to when a block is too old to be useless for IHC, that depends on the epitope and the way the blocks have been stored.
I have been able to use 25 years old blocks and? the only thing you have to do is to go deep?into the block to reach an area where the epitopes have not been oxidized yet.
Ren? J.

--- On Sat, 9/24/11, Andrea T. Hooper  wrote:


From: Andrea T. Hooper 
Subject: [Histonet] Block age/labile epitopes
To: "Histonet" 
Date: Saturday, September 24, 2011, 11:44 PM


Just curious as to at what shelf-life people consider their blocks too "old" to rely on for IHC data? Along those lines I am wondering about labile antigens ... does anyone know how fast epitopes may "disappear" from blocks and what are some good examples of labile epitopes/antigens? As usual any references or papers citations would be helpful.

Thank you!
Andrea Hooper
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From ldybas <@t> knox.edu  Sun Sep 25 12:33:35 2011
From: ldybas <@t> knox.edu (Linda Dybas)
Date: Sun Sep 25 12:33:49 2011
Subject: [Histonet] microwave processing
Message-ID: <4E7F65EF.5000306@knox.edu>

Does anyone have any experience using an EMS laboratory microwave for 
processing and/or staining tissue for light or electron microscopy?  I 
need help getting started.

Linda Dybas
Knox Collee
Galeesburg, IL



From mitchell <@t> wanpost.com  Sun Sep 25 15:44:46 2011
From: mitchell <@t> wanpost.com (Mitchell Wan)
Date: Sun Sep 25 15:44:56 2011
Subject: [Histonet] Medite TDO 66 operating manual
Message-ID: <002a01cc7bc3$f68da960$e3a8fc20$@com>

Hi All,

 

Does anyone have a pdf of the Medite TDO 66 operating manual?

 

 

Regards

 

Mitchell Wan

 

 

From megan.french <@t> mcri.edu.au  Sun Sep 25 18:40:36 2011
From: megan.french <@t> mcri.edu.au (Megan French)
Date: Sun Sep 25 18:40:46 2011
Subject: [Histonet] speed up fixation etc for cryomoulding
Message-ID: 

My current protocol for fixation and cryomoulding is as follows:

 

Day 1 zamboni's fixative 4deg overnight

Day 2 DMSO wash 3x10min, PBS wash 3x10min, store in PBS/30%sucrose 4 deg
overnight

Day 3 AM put tissue in cryomould with 50% PBS/30%sucrose & 50% OCT; PM
freeze in liquid nitrogen cooled isopentane.

 

I would like to shorten my protocol to a 2 day protocol without
jeopardising the quality of the tissue. I would appreciate any advice/
others protocols. I was thinking of freezing in liq nitrogen at the end
of day 2. Any advice is much appreciated.

 

Kind regards,

 

Megan French

 

Murdoch Children's Research Institute

Melbourne, Australia


______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
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From abright <@t> brightinstruments.com  Mon Sep 26 04:16:44 2011
From: abright <@t> brightinstruments.com (abright@brightinstruments.com)
Date: Mon Sep 26 04:19:35 2011
Subject: [Histonet] Thermo Shandon Histobathe
In-Reply-To: 
References: <49365679-58DD-48DE-80D5-0355BBA95515@buffalo.edu><1316614360.28378.YahooMailClassic@web65703.mail.ac4.yahoo.com>
Message-ID: <1192230583-1317028608-cardhu_decombobulator_blackberry.rim.net-314350455-@b26.c2.bise7.blackberry>




Dear Sir,

The Clin-RF Rapid Freezer for specimen preparation is part of a range we manufacture and sell through distributors in most regions of the world. The Clini-RF freezes down below -80 deg C using the latest non inflammable fluids or rapid conduction system.
Best regards

Alan Bright
Bright Instrument Co. Ltd.
St Margarets Way
Huntingdon
Cambridgeshire 
PE29 6EU
www.brightinsruments.com
Sent from my BlackBerry? wireless device

-----Original Message-----
From: "Nails, Felton" 
Sender: histonet-bounces@lists.utsouthwestern.edu
Date: Thu, 22 Sep 2011 09:18:48 
To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Thermo Shandon Histobathe

 
What are facilities using to replace their histobath in their frozen labs, since thermo no longer manufactures the histobath?

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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From amario3 <@t> uic.edu  Mon Sep 26 09:30:52 2011
From: amario3 <@t> uic.edu (Andrea Marion)
Date: Mon Sep 26 09:31:00 2011
Subject: [Histonet] Block age/labile epitopes
Message-ID: <27dd83b70466c5e884222985aee3a443.squirrel@webmail.uic.edu>

Hi Rene,

I am also interested in your article - could you send me a copy or provide
the reference?

Thanks,

Andrea

Andrea Marion
Graduate Student
University of Illinois at Chicago

Rene J Buesa rjbuesa <@t> yahoo.com
Sun Sep 25 10:36:24 CDT 2011
Previous message: [Histonet] Block age/labile epitopes
Next message: [Histonet] microwave processing
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
Andrea:
Under separate cover I am sending an article I wrote on the subject of
epitope oxidation including some  of theose easier to oxidize.
As to when a block is too old to be useless for IHC, that depends on the
epitope and the way the blocks have been stored.
I have been able to use 25 years old blocks and  the only thing you have
to do is to go deep into the block to reach an area where the epitopes
have not been oxidized yet.
Ren? J.

--- On Sat, 9/24/11, Andrea T. Hooper  rocketmail.com>
wrote:


From: Andrea T. Hooper  rocketmail.com>
Subject: [Histonet] Block age/labile epitopes
To: "Histonet"  lists.utsouthwestern.edu>
Date: Saturday, September 24, 2011, 11:44 PM


Just curious as to at what shelf-life people consider their blocks too
"old" to rely on for IHC data? Along those lines I am wondering about
labile antigens ... does anyone know how fast epitopes may "disappear"
from blocks and what are some good examples of labile epitopes/antigens?
As usual any references or papers citations would be helpful.

Thank you!
Andrea Hooper
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Previous message: [Histonet] Block age/labile epitopes
Next message: [Histonet] microwave processing
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
More information about the Histonet mailing list


From Rcartun <@t> harthosp.org  Mon Sep 26 10:00:08 2011
From: Rcartun <@t> harthosp.org (Richard Cartun)
Date: Mon Sep 26 10:00:18 2011
Subject: [Histonet] Block age/labile epitopes - UPDATE
In-Reply-To: <1316964984.7722.YahooMailClassic@web65702.mail.ac4.yahoo.com>
References: <1316922259.98459.YahooMailNeo@web113116.mail.gq1.yahoo.com>
	<1316964984.7722.YahooMailClassic@web65702.mail.ac4.yahoo.com>
Message-ID: <4E805B38.7400.0077.1@harthosp.org>

An interesting article on this subject came out earlier this year from
Stephen Hewitt's work group at the NCI.  Their work shows that the loss
of antigenicity in archival formalin-fixed, paraffin-embedded tissue
sections is due to the presence of water, both endogenously (poor
processing) and exogenously (humidity).  I've listed the reference
below:

Xie R, Chung J-Y, Ylaya K, et al.:  Factors influencing the degradation
of archival formalin-fixed, paraffin-embedded tissue sections.  J
Histochem Cytochem 59:356-365, 2011.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> Rene J Buesa  9/25/2011 11:36 AM >>>
Andrea:
Under separate cover I am sending an article I wrote on the subject of
epitope oxidation including some  of theose easier to oxidize.
As to when a block is too old to be useless for IHC, that depends on
the epitope and the way the blocks have been stored.
I have been able to use 25 years old blocks and  the only thing you
have to do is to go deep into the block to reach an area where the
epitopes have not been oxidized yet.
Ren? J.

--- On Sat, 9/24/11, Andrea T. Hooper 
wrote:


From: Andrea T. Hooper 
Subject: [Histonet] Block age/labile epitopes
To: "Histonet" 
Date: Saturday, September 24, 2011, 11:44 PM


Just curious as to at what shelf-life people consider their blocks too
"old" to rely on for IHC data? Along those lines I am wondering about
labile antigens ... does anyone know how fast epitopes may "disappear"
from blocks and what are some good examples of labile epitopes/antigens?
As usual any references or papers citations would be helpful.

Thank you!
Andrea Hooper
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From thisisann <@t> aol.com  Mon Sep 26 10:07:08 2011
From: thisisann <@t> aol.com (Ann Angelo)
Date: Mon Sep 26 10:07:19 2011
Subject: [Histonet] Glass Coverslipper
Message-ID: <8CE4A7517F24C5F-2264-103CA9@webmail-d178.sysops.aol.com>



I am looking to purchase a glass coverslipper in the near future (to coverslip approx. 1600 slides/day) and would like references from any of you that have an opinion (positive or negative).  Our slides have Ventana labels on them.....
Thank you,  Ann


From liz <@t> premierlab.com  Mon Sep 26 10:14:43 2011
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Mon Sep 26 10:16:07 2011
Subject: [Histonet] Glass Coverslipper
In-Reply-To: <8CE4A7517F24C5F-2264-103CA9@webmail-d178.sysops.aol.com>
Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA0A41@SBS2K8.premierlab.local>

We have the Sakura Glas coverslipper and we really like it. We still have some problems when it gets really humid in the lab and the coverglass will stick together, but overall it seems to work well for us.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo
Sent: Monday, September 26, 2011 9:07 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Glass Coverslipper



I am looking to purchase a glass coverslipper in the near future (to coverslip approx. 1600 slides/day) and would like references from any of you that have an opinion (positive or negative).  Our slides have Ventana labels on them.....
Thank you,  Ann


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From liz <@t> premierlab.com  Mon Sep 26 10:16:39 2011
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Mon Sep 26 10:18:01 2011
Subject: [Histonet] Block age/labile epitopes - UPDATE
In-Reply-To: <4E805B38.7400.0077.1@harthosp.org>
Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA0A42@SBS2K8.premierlab.local>

Does anyone have a pdf of this that they are willing to share.

Thanks

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Monday, September 26, 2011 9:00 AM
To: Histonet; Andrea T. Hooper; Rene J Buesa
Subject: Re: [Histonet] Block age/labile epitopes - UPDATE

An interesting article on this subject came out earlier this year from
Stephen Hewitt's work group at the NCI.  Their work shows that the loss
of antigenicity in archival formalin-fixed, paraffin-embedded tissue
sections is due to the presence of water, both endogenously (poor
processing) and exogenously (humidity).  I've listed the reference
below:

Xie R, Chung J-Y, Ylaya K, et al.:  Factors influencing the degradation
of archival formalin-fixed, paraffin-embedded tissue sections.  J
Histochem Cytochem 59:356-365, 2011.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> Rene J Buesa  9/25/2011 11:36 AM >>>
Andrea:
Under separate cover I am sending an article I wrote on the subject of
epitope oxidation including some  of theose easier to oxidize.
As to when a block is too old to be useless for IHC, that depends on
the epitope and the way the blocks have been stored.
I have been able to use 25 years old blocks and  the only thing you
have to do is to go deep into the block to reach an area where the
epitopes have not been oxidized yet.
Ren? J.

--- On Sat, 9/24/11, Andrea T. Hooper 
wrote:


From: Andrea T. Hooper 
Subject: [Histonet] Block age/labile epitopes
To: "Histonet" 
Date: Saturday, September 24, 2011, 11:44 PM


Just curious as to at what shelf-life people consider their blocks too
"old" to rely on for IHC data? Along those lines I am wondering about
labile antigens ... does anyone know how fast epitopes may "disappear"
from blocks and what are some good examples of labile epitopes/antigens?
As usual any references or papers citations would be helpful.

Thank you!
Andrea Hooper
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From gayle.callis <@t> bresnan.net  Mon Sep 26 10:33:16 2011
From: gayle.callis <@t> bresnan.net (gayle callis)
Date: Mon Sep 26 10:33:31 2011
Subject: [Histonet] So long, and thanks for all the help 
Message-ID: <000101cc7c61$9e3e0b80$daba2280$@bresnan.net>

Dear Adam, 

 

Thank you for the kind words.   It has always been a pleasure to pass histo
information onto others to help them with their projects.   As for dinner
and a drink, I would take you up on that - hopefully with Andrea there too.
In lieu of that, I raise my morning cup of coffee to you in congratulations
on your PhD, a huge achievement.   I would love to know the title of your
thesis and you can email that via private email.   I wish you great success
and hopefully a return to the Histonet network in the near future.  

 

Take care, invest in a good coffee maker to ward off sleep and enjoy your
medical studies.  

 

Gayle Callis       

From TJJ <@t> stowers.org  Mon Sep 26 10:44:08 2011
From: TJJ <@t> stowers.org (Johnson, Teri)
Date: Mon Sep 26 10:44:13 2011
Subject: [Histonet] Re: So long, and thanks for all the help
Message-ID: <2C40E43D1F7A56408C4463FD245DDDF985814021@EXCHMB-02.stowers-institute.org>

Adam,

I will miss your contribution on the Histonet. You have always been an asset to the listserv. I agree with your sentiments exactly, I know what I know because of the information I learned on here and from those kind souls willing to share. I continue to try to pay it forward and not get caught up in the insanity that breaks out from time to time.

Best of luck on your career path. I think you will do well.

Sincerely,

Teri Johnson, HT(ASCP)QIHC
Head, Histology and Electron Microscopy
Stowers Institute for Medical Research
Kansas City, MO


From gayle.callis <@t> bresnan.net  Mon Sep 26 10:52:15 2011
From: gayle.callis <@t> bresnan.net (gayle callis)
Date: Mon Sep 26 10:52:30 2011
Subject: [Histonet] Block age/labile epitopes - UPDATE
In-Reply-To: <14E2C6176416974295479C64A11CB9AE1DECBA0A42@SBS2K8.premierlab.local>
References: <4E805B38.7400.0077.1@harthosp.org>
	<14E2C6176416974295479C64A11CB9AE1DECBA0A42@SBS2K8.premierlab.local>
Message-ID: <000701cc7c64$45582480$d0086d80$@bresnan.net>

Liz, 

Pdf has been sent via private email.   

Gayle Callis 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chlipala
Sent: Monday, September 26, 2011 9:17 AM
To: 'Richard Cartun'; Histonet; Andrea T. Hooper; Rene J Buesa
Subject: RE: [Histonet] Block age/labile epitopes - UPDATE

Does anyone have a pdf of this that they are willing to share.

Thanks

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO
Box 18592 Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Monday, September 26, 2011 9:00 AM
To: Histonet; Andrea T. Hooper; Rene J Buesa
Subject: Re: [Histonet] Block age/labile epitopes - UPDATE

An interesting article on this subject came out earlier this year from
Stephen Hewitt's work group at the NCI.  Their work shows that the loss of
antigenicity in archival formalin-fixed, paraffin-embedded tissue sections
is due to the presence of water, both endogenously (poor
processing) and exogenously (humidity).  I've listed the reference
below:

Xie R, Chung J-Y, Ylaya K, et al.:  Factors influencing the degradation of
archival formalin-fixed, paraffin-embedded tissue sections.  J Histochem
Cytochem 59:356-365, 2011.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> Rene J Buesa  9/25/2011 11:36 AM >>>
Andrea:
Under separate cover I am sending an article I wrote on the subject of
epitope oxidation including some  of theose easier to oxidize.
As to when a block is too old to be useless for IHC, that depends on the
epitope and the way the blocks have been stored.
I have been able to use 25 years old blocks and  the only thing you have to
do is to go deep into the block to reach an area where the epitopes have not
been oxidized yet.
Ren? J.

--- On Sat, 9/24/11, Andrea T. Hooper 
wrote:


From: Andrea T. Hooper 
Subject: [Histonet] Block age/labile epitopes
To: "Histonet" 
Date: Saturday, September 24, 2011, 11:44 PM


Just curious as to at what shelf-life people consider their blocks too "old"
to rely on for IHC data? Along those lines I am wondering about labile
antigens ... does anyone know how fast epitopes may "disappear"
from blocks and what are some good examples of labile epitopes/antigens?
As usual any references or papers citations would be helpful.

Thank you!
Andrea Hooper
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From relia1 <@t> earthlink.net  Mon Sep 26 10:58:05 2011
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Mon Sep 26 10:58:05 2011
Subject: [Histonet] RELIA Hot Histology Job - 3 month assignment with a
	great lab in Beautiful Charlotte, NC
Message-ID: 

Hi Histonetters!
I hope you all are having a great day.  While I usually work exclusively
in permanent placement one of my very best clients has asked me to put
feelers out on their behalf because they are in need of a histotech for
a 3 month temporary assignment.  This is full time morning shift M-F.
If you or anyone you know might be interested in hearing more about it
please contact me.  I can be reached toll free at 866-607-3542 or
relia1@earthlink.net  
Thanks-Pam
Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.comPamBarkerRELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

From HornHV <@t> archildrens.org  Mon Sep 26 10:58:25 2011
From: HornHV <@t> archildrens.org (Horn, Hazel V)
Date: Mon Sep 26 10:58:32 2011
Subject: [Histonet] Glass Coverslipper
In-Reply-To: <8CE4A7517F24C5F-2264-103CA9@webmail-d178.sysops.aol.com>
References: <8CE4A7517F24C5F-2264-103CA9@webmail-d178.sysops.aol.com>
Message-ID: <25A4DE08332B19499904459F00AAACB719A93F385E@EVS1.archildrens.org>

We have a Leica CV5030 glass coverslipper and we like it. 

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo
Sent: Monday, September 26, 2011 10:07 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Glass Coverslipper



I am looking to purchase a glass coverslipper in the near future (to coverslip approx. 1600 slides/day) and would like references from any of you that have an opinion (positive or negative).  Our slides have Ventana labels on them.....
Thank you,  Ann


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

******************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************
The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the 
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message to the intended recipient, you are hereby notified that any 
dissemination, distribution or copying of this communication is strictly 
prohibited. If you have received this communication in error, please notify 
us immediately by replying to the message and deleting it from your computer.
Thank you.

From MSHERWOOD <@t> PARTNERS.ORG  Mon Sep 26 11:02:22 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret)
Date: Mon Sep 26 11:02:28 2011
Subject: [Histonet] Glass Coverslipper
In-Reply-To: <25A4DE08332B19499904459F00AAACB719A93F385E@EVS1.archildrens.org>
References: <8CE4A7517F24C5F-2264-103CA9@webmail-d178.sysops.aol.com>
	<25A4DE08332B19499904459F00AAACB719A93F385E@EVS1.archildrens.org>
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A1C@PHSXMB30.partners.org>

Ditto on the Leica CV5030. 


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Monday, September 26, 2011 11:58 AM
To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Glass Coverslipper

We have a Leica CV5030 glass coverslipper and we like it. 

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo
Sent: Monday, September 26, 2011 10:07 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Glass Coverslipper



I am looking to purchase a glass coverslipper in the near future (to coverslip
approx. 1600 slides/day) and would like references from any of you that have an
opinion (positive or negative).  Our slides have Ventana labels on them.....
Thank you,  Ann


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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The information contained in this message may be privileged and confidential
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From BDeBrosse-Serra <@t> isisph.com  Mon Sep 26 11:27:31 2011
From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra)
Date: Mon Sep 26 11:28:01 2011
Subject: [Histonet] Glass Coverslipper
In-Reply-To: <25A4DE08332B19499904459F00AAACB719A93F385E@EVS1.archildrens.org>
References: <8CE4A7517F24C5F-2264-103CA9@webmail-d178.sysops.aol.com>
	<25A4DE08332B19499904459F00AAACB719A93F385E@EVS1.archildrens.org>
Message-ID: <493CAA64F203E14E8823737B9EE0E25F091DED3E6C@EXCHMB01.isis.local>

I second that.

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
1896 Rutherford Road
Carlsbad, CA 92008
760-603-2371



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Monday, September 26, 2011 8:58 AM
To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Glass Coverslipper

We have a Leica CV5030 glass coverslipper and we like it. 

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Angelo
Sent: Monday, September 26, 2011 10:07 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Glass Coverslipper



I am looking to purchase a glass coverslipper in the near future (to coverslip approx. 1600 slides/day) and would like references from any of you that have an opinion (positive or negative).  Our slides have Ventana labels on them.....
Thank you,  Ann


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the 
intended recipient, or an employee or agent responsible for delivering this 
message to the intended recipient, you are hereby notified that any 
dissemination, distribution or copying of this communication is strictly 
prohibited. If you have received this communication in error, please notify 
us immediately by replying to the message and deleting it from your computer.
Thank you.

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From dvp.ipa <@t> hotmail.com  Mon Sep 26 12:10:25 2011
From: dvp.ipa <@t> hotmail.com (Deborah Ver Ploeg)
Date: Mon Sep 26 12:10:28 2011
Subject: [Histonet] BenchMark XT HPV protocols
Message-ID: 


I need to move my HPV 6+ and HPV 16+ from my baby BenchMark to my BenchMark XT. Is there anyone willing to share their protocols?
Thanks. 		 	   		  
From TJJ <@t> stowers.org  Mon Sep 26 12:21:34 2011
From: TJJ <@t> stowers.org (Johnson, Teri)
Date: Mon Sep 26 12:21:38 2011
Subject: [Histonet] Incredible job opportunity - Kansas City, MO
Message-ID: <2C40E43D1F7A56408C4463FD245DDDF985814027@EXCHMB-02.stowers-institute.org>

I am reposting this in case it was missed earlier. The Stowers Institute is looking for someone with a solid background in Histology, including research histology and supervisory experience to head the Histology and Electron Microscopy Core Facility. I will post the actual ad below, but before I do just let me say that working at this facility is a once-in-a-lifetime experience. I urge you to visit the website, www.stowers.org, and read about the type of reseach they are doing here, and learn more about the Institute and their mission. If you have been considering a career move, take and look and see if this is something that interests you.

This is a challenging but incredibly rewarding position. You will learn something new every day and contribute to really great science. Yes, this is my position they are replacing. It has been a great 10 years here, and whomever takes over will be managing a staff of really talented histology and electron microscopy specialists. I am leaving due to personal reasons, it has nothing whatsoever to do with the management, staff, or any issue I have with the Institute. Trust me, it was very difficult to make the decision to leave. This is by far the best job I have ever had.

I will be moving onward and forward and will still be a regular contributor to the histonet after starting my new position next month at GNF (Genomics Institute for Novartis Foundation). I am looking forward to the challenge.

Teri Johnson, HT(ASCP)QIHC
Head, Histology and Electron Microscopy
Stowers Institute for Medical Research
Kansas City, MO


The Stowers Institute for Medical Research has an opening for a Head of Histology and Electron Microscopy to oversee expert delivery of the highest quality service for the detection of gene and protein expression in tissue samples; histochemical staining; sample fixation; routine histology; and ultra-structural analysis.

 Primary responsibilities include maintaining the current status of projects and resolving issues in the EM and Histology labs; actively promoting team interaction and participation; monitoring workload and turn-around time through the LIMS system; providing oversight and feedback on complex or non-routine projects; monitoring usage of services; responding appropriately to unexpected peaks in workload and service requests; maintaining effective communication with all members of the Institute; troubleshooting problems and communicating appropriately with Principal Investigators and the scientific staff, administration, and other core facility personnel as needed; making formal and informal presentations on Core Center services; ensuring continuing education of staff through workshops, webinars, lab meetings, and email communications; and reading professional journals and other sources to stay current in Histologic and EM techniques.

In addition to outstanding communication skills and the ability to effectively multi-task in a team-oriented environment, the successful candidate will have QIHC (ASCP) Qualification, and HT or HTL certification.  Previous management or supervisory experience is preferred.

Minimum requirements include an undergraduate degree in the sciences; excellent knowledge of histologic technique to include fixation, processing, histochemical staining, immunohistochemical staining, microtomy (to include cryostat, paraffin, and plastic), and ultramicrotomy; experience in a research environment with animal model histology; and the ability to apply good judgement to troubleshooting, problem solving, and staff management.  Five to ten years relevant Histology and/or Electron Microscopy experience in lieu of education may be considered.

To apply, send resume and coverletter to careers@stowers.org




From Margaret.Perry <@t> sdstate.edu  Mon Sep 26 12:22:26 2011
From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret)
Date: Mon Sep 26 12:22:31 2011
Subject: [Histonet] nonstructured proteins
Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE012E34@SDSU-EX03.jacks.local>

I am posting this again to see if anyone has worked with nonstructured proteins.  Do they form the same crosslinks when fixed with formalin?  Would antigen retrieval be of any use?  Do they react with IHC the same as structured proteins?


Margaret Perry HT(ASCP)
Dept of Veterinary and  Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638

From gu.lang <@t> gmx.at  Mon Sep 26 13:24:59 2011
From: gu.lang <@t> gmx.at (Gudrun Lang)
Date: Mon Sep 26 13:31:50 2011
Subject: AW: [Histonet] nonstructured proteins
In-Reply-To: <25F4FBA34BE9D142964ECC4525B82AEE012E34@SDSU-EX03.jacks.local>
References: <25F4FBA34BE9D142964ECC4525B82AEE012E34@SDSU-EX03.jacks.local>
Message-ID: <98E7BB93183D4B729EE437D4CCB0AECB@dielangs.at>

After a short look into wikipedia I assume, that these proteins can also
bind formaldehyde as long as there are aminogroups of lysin and arginine
reachable. Crosslinking would be possible, if bound methylgroups come near
enough to peptidbinding. If this occurs intra- or intermolecular, who knows?

Do you build your own antibodies?

Gudrun
 

-----Urspr?ngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Perry,
Margaret
Gesendet: Montag, 26. September 2011 19:22
An: ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu
Betreff: [Histonet] nonstructured proteins

I am posting this again to see if anyone has worked with nonstructured
proteins.  Do they form the same crosslinks when fixed with formalin?  Would
antigen retrieval be of any use?  Do they react with IHC the same as
structured proteins?


Margaret Perry HT(ASCP)
Dept of Veterinary and  Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From andrea.conard <@t> gmail.com  Mon Sep 26 13:55:25 2011
From: andrea.conard <@t> gmail.com (andrea conard)
Date: Mon Sep 26 13:55:29 2011
Subject: [Histonet] benchmarking histo processes
Message-ID: 

Hi all,
we are a histo lab doing about K14/year. There are 5.5 histotechs and six
pathologists. The grossing is done by one PA assisted by one tech aid.
Biopsies are processed on the microwave processor everything else gets done
overnight. Pathologists start getting slides at 9AM and have the last tray
by 11AM. We do about 500 IHC/month. Our health system has two campuses but
histo is only done at one campus. Specimens from the second campus get
logged into the system and packed up by an assistant transported by courier
to the campus with histo operations.
I'm looking for other institutions with a similar set up to see what time
their slides come out, what the start and endtimes are for the techs, etc.
From andrea.conard <@t> gmail.com  Mon Sep 26 14:00:22 2011
From: andrea.conard <@t> gmail.com (andrea conard)
Date: Mon Sep 26 14:00:27 2011
Subject: [Histonet] bench marking histo process
Message-ID: 

Hi all,
we are a histo lab doing about K14/year. There are 5.5 histotechs and six
pathologists. The grossing is done by one PA assisted by one tech aid.
Biopsies are processed on the microwave processor everything else gets done
overnight. Pathologists start getting slides at 9AM and have the last tray
by 11AM. We do about 500 IHC/month. Our health system has two campuses but
histo is only done at one campus. Specimens from the second campus get
logged into the system and packed up by an assistant transported by courier
to the campus with histo operations.
I'm looking for other institutions with a similar set up to see what time
their slides come out, what the start and endtimes are for the techs, etc.
you can send responses to andrea.conard@atlanticare.org  or use the server.

Thanks for your help,

*Andrea Conard, HT(ASCP)*

*Pathology Supervisor*

*AtlantiCare** Regional Medical Center***

*Phone: 609-441-8168*

*Fax:609-441-2195*
From aaperghis <@t> uspath.com  Mon Sep 26 14:09:45 2011
From: aaperghis <@t> uspath.com (Adrienne Aperghis Kavanagh)
Date: Mon Sep 26 14:10:14 2011
Subject: [Histonet] RE:  Stain Precipitate (H.Pylori)
Message-ID: <12823886.222925.1317064185836.JavaMail.root@mail3d.brinkster.com>

We use H.Pylori from Cell Marque and the Benchmark XT as well.  When we began doing the H.Pylori by immuno (versus Giemsa) we had had the same problem.  Tech support from Cell Marque suggested using a Protease 2 (from Ventana) to decrese background, and it worked.  Here's our protocol:

CC1 Mild (30 min)
Protease 2 (4 min)
H.Pylori (32 min)
Hematox (4 min)
Bluing (4 min)

Hope this helps!!
-- 



Adrienne Kavanagh? HTL (ASCP) 
US PATH 
30 W. Century Road 
Suite 255 
Paramus NJ 07652 

From Margaret.Perry <@t> sdstate.edu  Mon Sep 26 14:16:41 2011
From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret)
Date: Mon Sep 26 14:16:46 2011
Subject: [Histonet] looking for
Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE012E91@SDSU-EX03.jacks.local>

Does anyone have an e-mail address for Dick Dapson, Clive Taylor, and William Grizzle?

Margaret Perry HT(ASCP)
Dept of Veterinary and  Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638

From roosmith1 <@t> hotmail.com  Mon Sep 26 19:13:04 2011
From: roosmith1 <@t> hotmail.com (Jackie Smith)
Date: Mon Sep 26 19:13:09 2011
Subject: [Histonet] Kappa/ Lambda ISH Probes on BOND III
Message-ID: 


Histonetters... 
Would anyone performing ISH probes for Kappa & Lambda on the BOND III be willing to share their protocol for B+ fixed, decaled, paraffin-embedded bone marrow core sections.  I have tried a variety of different combinations and times with ER1, ER2, Enzyme & incubation, yielding only minimal, suboptimal, quality staining.  
Any input is greatly appreciated! 
Kindest Regards,  Eric SmithCelligent DiagnosticsCharlotte, NC 		 	   		  
From djohnson <@t> mercedesmedical.com  Tue Sep 27 07:53:48 2011
From: djohnson <@t> mercedesmedical.com (Dave Johnson)
Date: Tue Sep 27 07:54:44 2011
Subject: [Histonet] Dating for Alcohol
Message-ID: <0877540BC955334DA70CD09476895D6B556E358B60@mmiex07.mercedesmedical.com>

I have heard confliciting opinions.

Do Alcohol require an expiration date?  Does anyone have a link to the regs on this

Thanks
Dave Johnson
Mercedes Medical


From jqb7 <@t> cdc.gov  Tue Sep 27 07:57:06 2011
From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID))
Date: Tue Sep 27 07:57:41 2011
Subject: [Histonet] RE: Dating for Alcohol
In-Reply-To: <0877540BC955334DA70CD09476895D6B556E358B60@mmiex07.mercedesmedical.com>
References: <0877540BC955334DA70CD09476895D6B556E358B60@mmiex07.mercedesmedical.com>
Message-ID: 

I thought this email was about dating so you can get alcohol.....as in "dating"..........LOL!

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dave Johnson
Sent: Tuesday, September 27, 2011 8:54 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Dating for Alcohol

I have heard confliciting opinions.

Do Alcohol require an expiration date?  Does anyone have a link to the regs on this

Thanks
Dave Johnson
Mercedes Medical


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From rjbuesa <@t> yahoo.com  Tue Sep 27 08:13:08 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Sep 27 08:13:11 2011
Subject: [Histonet] Dating for Alcohol
In-Reply-To: <0877540BC955334DA70CD09476895D6B556E358B60@mmiex07.mercedesmedical.com>
Message-ID: <1317129188.66148.YahooMailClassic@web65714.mail.ac4.yahoo.com>

Alcohol will absorb water vapor from the atmosphere and if the container?is not properly closed will degrade from 100% (200 proof). If you start with 95% (190 proof) that is not a very big problem.
Reagent alcohol (we are referring to ethanol, of course) that needs to be used pure,?is usually stored with added anhydrous copper sulfate that will settle on the bottom of the flask and will turn from white to blue while absorbind the water that slowly contaminates tha?ethanol. Usually you will not need it so pure.
The answer to your question is NO, ethanol does not need an experimation date. In histology you will never need it to such purity level.
Complete dehydration of tissues can be achieved with "less than pure" ethanol.
Ren? J.

--- On Tue, 9/27/11, Dave Johnson  wrote:


From: Dave Johnson 
Subject: [Histonet] Dating for Alcohol
To: "'histonet@lists.utsouthwestern.edu'" 
Date: Tuesday, September 27, 2011, 8:53 AM


I have heard confliciting opinions.

Do Alcohol require an expiration date?? Does anyone have a link to the regs on this

Thanks
Dave Johnson
Mercedes Medical


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From SDattili <@t> stormontvail.org  Tue Sep 27 08:27:19 2011
From: SDattili <@t> stormontvail.org (D'Attilio, Shelley)
Date: Tue Sep 27 08:27:31 2011
Subject: [Histonet] Xylene sensitivity
Message-ID: 

Hi all,
I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper).  This is her first job in a lab of any sort.  We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc.  Right now she is wearing a PAPR to work, which is obviously not a long-term solution.  Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?  I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour.  It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment.  Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings.  We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors.  I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas
 



NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.  Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.  If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.  Thank you for your cooperation.

******************************************************************************************************************
From rjbuesa <@t> yahoo.com  Tue Sep 27 08:33:36 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Sep 27 08:33:44 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
Message-ID: <1317130416.25049.YahooMailClassic@web65710.mail.ac4.yahoo.com>

Shelley:
As you correctly think this employee is most likely sensitive to xylene. Having a filtration system?is the wrong solution.
Is like having a pain when you use your arm and immobilizing it to prevent the pain.
Your long term and complete solution is to eliminate xylene from your lab and I mean from ALL tasks using it now.
Under separate cover I am sending 2 publications explaining how to do that.
Ren? J.

--- On Tue, 9/27/11, D'Attilio, Shelley  wrote:


From: D'Attilio, Shelley 
Subject: [Histonet] Xylene sensitivity
To: "Histonet Listserv (E-mail)" 
Date: Tuesday, September 27, 2011, 9:27 AM


Hi all,
I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper).? This is her first job in a lab of any sort.? We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc.? Right now she is wearing a PAPR to work, which is obviously not a long-term solution.? Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour.? It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment.? Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings.? We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors.? I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?? Stormont-Vail's Health Connections can help you find a doctor accepting new patients.? Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.? Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.? If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.? Thank you for your cooperation.

******************************************************************************************************************
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From Loralee_Mcmahon <@t> URMC.Rochester.edu  Tue Sep 27 08:40:41 2011
From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A)
Date: Tue Sep 27 08:41:45 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: <1317130416.25049.YahooMailClassic@web65710.mail.ac4.yahoo.com>
References: ,
	<1317130416.25049.YahooMailClassic@web65710.mail.ac4.yahoo.com>
Message-ID: 

Rene, 

I understand the desire to remove Xylene from the lab completely.  But what if you are running FDA approved kits that are only FDA approved if the tissue is processed in Xylene? 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:33 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: Re: [Histonet] Xylene sensitivity

Shelley:
As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution.
Is like having a pain when you use your arm and immobilizing it to prevent the pain.
Your long term and complete solution is to eliminate xylene from your lab and I mean from ALL tasks using it now.
Under separate cover I am sending 2 publications explaining how to do that.
Ren? J.

--- On Tue, 9/27/11, D'Attilio, Shelley  wrote:


From: D'Attilio, Shelley 
Subject: [Histonet] Xylene sensitivity
To: "Histonet Listserv (E-mail)" 
Date: Tuesday, September 27, 2011, 9:27 AM


Hi all,
I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper).  This is her first job in a lab of any sort.  We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc.  Right now she is wearing a PAPR to work, which is obviously not a long-term solution.  Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?  I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour.  It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment.  Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings.  We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors.  I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.  Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.  If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.  Thank you for your cooperation.

******************************************************************************************************************
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
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From rjbuesa <@t> yahoo.com  Tue Sep 27 08:54:48 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Sep 27 08:54:51 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
Message-ID: <1317131688.12777.YahooMailClassic@web65703.mail.ac4.yahoo.com>

Loralee:
Please enlighten me! As far as I know FDA requirements extend to fixation time?and NBF is required by some kit FDA approved, but not to how the tissues are processed.
Even there are studies that show that xylene has extracting effects over some epitopes.
I know that you cannot argue with FDA, but you can always validate any changes in your procedure.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:


From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 9:40 AM


Rene, 

I understand the desire to remove Xylene from the lab completely.? But what if you are running FDA approved kits that are only FDA approved if the tissue is processed in Xylene? 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:33 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: Re: [Histonet] Xylene sensitivity

Shelley:
As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution.
Is like having a pain when you use your arm and immobilizing it to prevent the pain.
Your long term and complete solution is to eliminate xylene from your lab and I mean from ALL tasks using it now.
Under separate cover I am sending 2 publications explaining how to do that.
Ren? J.

--- On Tue, 9/27/11, D'Attilio, Shelley  wrote:


From: D'Attilio, Shelley 
Subject: [Histonet] Xylene sensitivity
To: "Histonet Listserv (E-mail)" 
Date: Tuesday, September 27, 2011, 9:27 AM


Hi all,
I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper).? This is her first job in a lab of any sort.? We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc.? Right now she is wearing a PAPR to work, which is obviously not a long-term solution.? Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour.? It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment.? Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings.? We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors.? I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?? Stormont-Vail's Health Connections can help you find a doctor accepting new patients.? Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.? Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.? If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.? Thank you for your cooperation.

******************************************************************************************************************
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From thiggins <@t> cddmedical.com  Tue Sep 27 09:50:31 2011
From: thiggins <@t> cddmedical.com (Tim Higgins)
Date: Tue Sep 27 09:50:39 2011
Subject: [Histonet] p57 kip2 (Ab3) concentrated antibody
Message-ID: <006c01cc7d24$ce39f060$e001a8c0@cdd.loc>

Does anyone use the p57 (Ab3) concentrated antibody from Lab Vision (Fisher) for Molar pregnancies on the Ventana platform using the UltraView detection system?  What is the protocol and dilution you run?


Any help would be greatly appreciated!!

Thanks,


Timothy Higgins, HT(ASCP), QIHC
Histology Supervisor
CDD
From Loralee_Mcmahon <@t> URMC.Rochester.edu  Tue Sep 27 09:57:09 2011
From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A)
Date: Tue Sep 27 09:57:18 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: <1317131688.12777.YahooMailClassic@web65703.mail.ac4.yahoo.com>
References: ,
	<1317131688.12777.YahooMailClassic@web65703.mail.ac4.yahoo.com>
Message-ID: 

The Hercept Test from Dako specifies  xylene in the breast tissue processing section.  The Er/Pr Pharm DX kit may also, but I haven't check that out.   I know this because we just fought with a sister hospital to get them to change their processors to xylene.


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

________________________________
From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:54 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity

Loralee:
Please enlighten me! As far as I know FDA requirements extend to fixation time and NBF is required by some kit FDA approved, but not to how the tissues are processed.
Even there are studies that show that xylene has extracting effects over some epitopes.
I know that you cannot argue with FDA, but you can always validate any changes in your procedure.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:

From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 9:40 AM

Rene,

I understand the desire to remove Xylene from the lab completely.  But what if you are running FDA approved kits that are only FDA approved if the tissue is processed in Xylene?

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:33 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: Re: [Histonet] Xylene sensitivity

Shelley:
As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution.
Is like having a pain when you use your arm and immobilizing it to prevent the pain.
Your long term and complete solution is to eliminate xylene from your lab and I mean from ALL tasks using it now.
Under separate cover I am sending 2 publications explaining how to do that.
Ren? J.

--- On Tue, 9/27/11, D'Attilio, Shelley > wrote:


From: D'Attilio, Shelley >
Subject: [Histonet] Xylene sensitivity
To: "Histonet Listserv (E-mail)" >
Date: Tuesday, September 27, 2011, 9:27 AM


Hi all,
I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper).  This is her first job in a lab of any sort.  We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc.  Right now she is wearing a PAPR to work, which is obviously not a long-term solution.  Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?  I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour.  It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment.  Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings.  We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors.  I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.  Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.  If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.  Thank you for your cooperation.

******************************************************************************************************************
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From ihcman2010 <@t> hotmail.com  Tue Sep 27 10:11:29 2011
From: ihcman2010 <@t> hotmail.com (Glen Dawson)
Date: Tue Sep 27 10:11:33 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
References: 
Message-ID: 


Shelley,
 
Perhaps this employee should look into other job opportunities.  The histo lab is filled with chemicals that can cause sensitivity in those people who may be prone to that.  Unfortunately, some of the main players in the histo lab (xylene & formalin) are often the culprit, but they are the best products in terms of how well they work, how universally they are used, how well their characteristics are known, etc...  I hate to sound like I'm not sympathetic, because I truly am, but I think it is a mistake to modify a histology lab's procedures to accomodate a new employee.
 
Just my opinion,
 
Glen Dawson  BS, HT(ASCP) & QIHC
Milwaukee, WI
 

> Date: Tue, 27 Sep 2011 08:27:19 -0500
> From: SDattili@stormontvail.org
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Xylene sensitivity
> 
> Hi all,
> I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper). This is her first job in a lab of any sort. We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc. Right now she is wearing a PAPR to work, which is obviously not a long-term solution. Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.
> 
> Does anyone have any experience with activated charcoal air cleaners? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour. It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment. Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings. We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors. I am open to any suggestions.
> 
> Thanks,
> 
> Shelley D'Attilio MT(ASCP)
> Manager, Chemistry, Cytology and Histology
> Dept. of Pathology and Laboratory Medicine
> Stormont-Vail HealthCare
> Topeka, Kansas
> 
> 
> 
> 
> NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225.
> 
> ******************************************************************************************************************
> 
> The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation.
> 
> ******************************************************************************************************************
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From Carmen.M.Garcia <@t> uv.es  Tue Sep 27 10:14:51 2011
From: Carmen.M.Garcia <@t> uv.es (Carmen Maria Garcia Pascual)
Date: Tue Sep 27 10:15:04 2011
Subject: [Histonet] Paraffine-embedded tissue
Message-ID: <4213787553carmaga6@uv.es>



Hello everyone:
 
I am Hortensia from the University of Valencia, from Spain. I am new in 
this world. And I have some issues. I will apreciatte if someone with 
experience can help me. 
I am working with endometrial tissue samples, from biopses and 
endometrial aspirates. I want to detect the nervous fibres with 9.5PGP 
antibody (from DAKO). I am using as positive control large intestine, 
and I am getting a very clear staining. But when I perform the 
immunohistochemistry in the endometrial samples I don't obtain any 
staining. so, I think that the problem may  be in the previous steps, 
like the pick of the sample, the fixation  and/or the paraffine 
incluison protocol. I fix the samples 12-24hs in formaline 10%, the 
paraffine inclusion protocol is the next: etoh 70%, 96% and 100% (2 
changes 1 hr each); xilene 2 changes x 30 min and 1,5hr twice in 
paraffine, then I cut the sample.
I want to know if this protocol is correct and if the samples are very 
small, should I decreased the incubations times at the half?
In summarize, which is your paraffine inclusion protocol? and this 
protocol is independent of the sample size?
 
Thank you very much!!!!!
Best regards,
Horten
 		 	   		  


From nicole <@t> dlcjax.com  Tue Sep 27 10:21:49 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Tue Sep 27 10:27:44 2011
Subject: [Histonet] Facebook
Message-ID: <2100.208.62.167.196.1317136909.squirrel@webmail.realpages.com>

Hello Histonetters,


Just drooping in to say thanks to all those Who have joined Histo-ville on
facebook. We are forming an awesome community and hope more will join.
Stop by and like us.

Nicole Tatum, HT ASCP


From rjbuesa <@t> yahoo.com  Tue Sep 27 10:35:07 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Sep 27 10:35:16 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
Message-ID: <1317137707.83978.YahooMailClassic@web65703.mail.ac4.yahoo.com>

Loralee:
Poor people?in your sister hospital! You are affecting their health. I would have approached a different way and never done that.
DAKO cannot dictate how I process my samples. I would have gotten involved my legal department to handle that. 
I would have requested the experimental data from DAKO. They obtained approval from FDA using their process. They submitted that process that unfortunately included using xylene but that in no way invalidates the Herpset Tests results.
Are you going to tell me that the results of your sister hospital were wrong while using something other than xylene??I would have interchanged pieces of tissue and evaluate the results.
I would have demonstrated that there were no differences and I say this because that is what I did when processing with mineral oil at my hospital. The whole process was validated and we also did Hercept test from DAKO.
Do they also require using ethanol? Does it matter if you use 2-propanol?
Why limiting the process to just about xylene and not about the dehydrating agent?
Do they also try to determine the lenght of the process?
I think that accepting DAKO protocol without challenging it and forcing your sister hospital to expose their employees to xylene was absolutely wrong!
Forgive me to say this, but you took the "easy way" instead of challenging the whole issue and demonstrate that their requirement from the technical point of view absolutely baseless.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:


From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 10:57 AM








The?Hercept Test from?Dako specifies??xylene in the breast tissue processing section.? The Er/Pr?Pharm DX kit may also, but I haven't check that out.?? I know this because we just fought with a sister hospital to get them to change their processors to xylene.? 
?
?

Loralee?McMahon,?HTL (ASCP)
Immunohistochemistry?Supervisor
Strong Memorial Hospital 
Department of Surgical Pathology
(585) 275-7210
?


From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:54 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity








Loralee:
Please enlighten me! As far as I know FDA requirements extend to fixation time?and NBF is required by some kit FDA approved, but not to how the tissues are processed.
Even there are studies that show that xylene has extracting effects over some epitopes.
I know that you cannot argue with FDA, but you can always validate any changes in your procedure.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:


From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 9:40 AM


Rene, 

I understand the desire to remove Xylene from the lab completely.? But what if you are running FDA approved kits that are only FDA approved if the tissue is processed in Xylene? 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:33 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: Re: [Histonet] Xylene sensitivity

Shelley:
As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution.
Is like having a pain when you use your arm and immobilizing it to prevent the pain.
Your long term and complete solution is to eliminate xylene from your lab and I mean from ALL tasks using it now.
Under separate cover I am sending 2 publications explaining how to do that.
Ren? J.

--- On Tue, 9/27/11, D'Attilio, Shelley  wrote:


From: D'Attilio, Shelley 
Subject: [Histonet] Xylene sensitivity
To: "Histonet Listserv (E-mail)" 
Date: Tuesday, September 27, 2011, 9:27 AM


Hi all,
I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper).? This is her first job in a lab of any sort.? We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc.? Right now she is wearing a PAPR to work, which is obviously not a long-term solution.? Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour.? It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment.? Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings.? We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors.? I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?? Stormont-Vail's Health Connections can help you find a doctor accepting new patients.? Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.? Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.? If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.? Thank you for your cooperation.

******************************************************************************************************************
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From rjbuesa <@t> yahoo.com  Tue Sep 27 10:44:35 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Sep 27 10:44:43 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
Message-ID: <1317138275.75985.YahooMailClassic@web65709.mail.ac4.yahoo.com>

Glen:
I respectfully disagree. Do you have any idea what would mean to that employee to try to get another job NOW?
Changing the procedure is what we (as histotechnique refers to) have been doing for ever.
Perhaps you do not remember other methods, but I do because I started working in this?field in 1952.
Back then I processed tissues manually and used aniline oil to "clear", benzene? as ante medium and was very glad when I switched to white gasoline and later to xylene.
Do you know why xylene became so prevalent? Because in 1943 the AutioTechnicon was developed and they recommended xylene as the way to go.
That selection also was the choice of the VIP manufacturers, and we all started to change from the toxic benzene and aniline oil to the toxic xylene.
There are other ways of doing things and changing work or exposing to toxic chemicals is not the correct path, no matter how much we are "used to".
Ren? J.

--- On Tue, 9/27/11, Glen Dawson  wrote:


From: Glen Dawson 
Subject: RE: [Histonet] Xylene sensitivity
To: sdattili@stormontvail.org, histonet@lists.utsouthwestern.edu
Date: Tuesday, September 27, 2011, 11:11 AM



Shelley,

Perhaps this employee should look into other job opportunities.? The histo lab is filled with chemicals that can cause sensitivity in those people who may be prone to that.? Unfortunately, some of the main players in the histo lab (xylene & formalin) are often the culprit, but they are the best products in terms of how well they work, how universally they are used, how well their characteristics are known, etc...? I hate to sound like I'm not sympathetic, because I truly am, but I think it is a mistake to modify a histology lab's procedures to accomodate a new employee.

Just my opinion,

Glen Dawson? BS, HT(ASCP) & QIHC
Milwaukee, WI


> Date: Tue, 27 Sep 2011 08:27:19 -0500
> From: SDattili@stormontvail.org
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Xylene sensitivity
> 
> Hi all,
> I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper). This is her first job in a lab of any sort. We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc. Right now she is wearing a PAPR to work, which is obviously not a long-term solution. Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.
> 
> Does anyone have any experience with activated charcoal air cleaners? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour. It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment. Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings. We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors. I am open to any suggestions.
> 
> Thanks,
> 
> Shelley D'Attilio MT(ASCP)
> Manager, Chemistry, Cytology and Histology
> Dept. of Pathology and Laboratory Medicine
> Stormont-Vail HealthCare
> Topeka, Kansas
> 
> 
> 
> 
> NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225.
> 
> ******************************************************************************************************************
> 
> The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation.
> 
> ******************************************************************************************************************
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
??? ???????? ?????? ??? ? _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From KCross <@t> cvm.tamu.edu  Tue Sep 27 10:49:20 2011
From: KCross <@t> cvm.tamu.edu (Kelly Cross)
Date: Tue Sep 27 10:49:28 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: <1317137707.83978.YahooMailClassic@web65703.mail.ac4.yahoo.com>
References: 
	<1317137707.83978.YahooMailClassic@web65703.mail.ac4.yahoo.com>
Message-ID: <869848DDBB7C5D4896A569A38B814E690551947E@CVMMB01.cvm.tamu.edu>

I have gone around and around with DAKO because they want to blame the fact that I use Pro-Par rather than xylene in my lab. I use it for  processing and de-paraffinizing and some of their people want to blame that on their Artisan Link Auto Stainer staining inconsistently in my lab. . . I like xylene, but it sure is rough on some people! 

Kelly Cross

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, September 27, 2011 10:35 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; Loralee AMcMahon
Subject: RE: [Histonet] Xylene sensitivity

Loralee:
Poor people?in your sister hospital! You are affecting their health. I would have approached a different way and never done that.
DAKO cannot dictate how I process my samples. I would have gotten involved my legal department to handle that. 
I would have requested the experimental data from DAKO. They obtained approval from FDA using their process. They submitted that process that unfortunately included using xylene but that in no way invalidates the Herpset Tests results.
Are you going to tell me that the results of your sister hospital were wrong while using something other than xylene??I would have interchanged pieces of tissue and evaluate the results.
I would have demonstrated that there were no differences and I say this because that is what I did when processing with mineral oil at my hospital. The whole process was validated and we also did Hercept test from DAKO.
Do they also require using ethanol? Does it matter if you use 2-propanol?
Why limiting the process to just about xylene and not about the dehydrating agent?
Do they also try to determine the lenght of the process?
I think that accepting DAKO protocol without challenging it and forcing your sister hospital to expose their employees to xylene was absolutely wrong!
Forgive me to say this, but you took the "easy way" instead of challenging the whole issue and demonstrate that their requirement from the technical point of view absolutely baseless.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:


From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 10:57 AM








The?Hercept Test from?Dako specifies??xylene in the breast tissue processing section.? The Er/Pr?Pharm DX kit may also, but I haven't check that out.?? I know this because we just fought with a sister hospital to get them to change their processors to xylene.? 
?
?

Loralee?McMahon,?HTL (ASCP)
Immunohistochemistry?Supervisor
Strong Memorial Hospital 
Department of Surgical Pathology
(585) 275-7210
?


From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:54 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity








Loralee:
Please enlighten me! As far as I know FDA requirements extend to fixation time?and NBF is required by some kit FDA approved, but not to how the tissues are processed.
Even there are studies that show that xylene has extracting effects over some epitopes.
I know that you cannot argue with FDA, but you can always validate any changes in your procedure.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:


From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 9:40 AM


Rene, 

I understand the desire to remove Xylene from the lab completely.? But what if you are running FDA approved kits that are only FDA approved if the tissue is processed in Xylene? 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:33 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: Re: [Histonet] Xylene sensitivity

Shelley:
As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution.
Is like having a pain when you use your arm and immobilizing it to prevent the pain.
Your long term and complete solution is to eliminate xylene from your lab and I mean from ALL tasks using it now.
Under separate cover I am sending 2 publications explaining how to do that.
Ren? J.

--- On Tue, 9/27/11, D'Attilio, Shelley  wrote:


From: D'Attilio, Shelley 
Subject: [Histonet] Xylene sensitivity
To: "Histonet Listserv (E-mail)" 
Date: Tuesday, September 27, 2011, 9:27 AM


Hi all,
I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper).? This is her first job in a lab of any sort.? We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc.? Right now she is wearing a PAPR to work, which is obviously not a long-term solution.? Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour.? It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment.? Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings.? We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors.? I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?? Stormont-Vail's Health Connections can help you find a doctor accepting new patients.? Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.? Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.? If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.? Thank you for your cooperation.

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From KCross <@t> cvm.tamu.edu  Tue Sep 27 10:51:43 2011
From: KCross <@t> cvm.tamu.edu (Kelly Cross)
Date: Tue Sep 27 10:51:47 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: <1317138275.75985.YahooMailClassic@web65709.mail.ac4.yahoo.com>
References: 
	<1317138275.75985.YahooMailClassic@web65709.mail.ac4.yahoo.com>
Message-ID: <869848DDBB7C5D4896A569A38B814E69055194A2@CVMMB01.cvm.tamu.edu>

My best friend just lost a job not too long ago because she was accused of NOT telling the lady she interviewed with that she had a sensitivity to xylene! I know she had to have told her because she hasn't worked with xylene in 20 years! 
Kelly

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, September 27, 2011 10:45 AM
To: sdattili@stormontvail.org; histonet@lists.utsouthwestern.edu; Glen Dawson
Subject: RE: [Histonet] Xylene sensitivity

Glen:
I respectfully disagree. Do you have any idea what would mean to that employee to try to get another job NOW?
Changing the procedure is what we (as histotechnique refers to) have been doing for ever.
Perhaps you do not remember other methods, but I do because I started working in this?field in 1952.
Back then I processed tissues manually and used aniline oil to "clear", benzene? as ante medium and was very glad when I switched to white gasoline and later to xylene.
Do you know why xylene became so prevalent? Because in 1943 the AutioTechnicon was developed and they recommended xylene as the way to go.
That selection also was the choice of the VIP manufacturers, and we all started to change from the toxic benzene and aniline oil to the toxic xylene.
There are other ways of doing things and changing work or exposing to toxic chemicals is not the correct path, no matter how much we are "used to".
Ren? J.

--- On Tue, 9/27/11, Glen Dawson  wrote:


From: Glen Dawson 
Subject: RE: [Histonet] Xylene sensitivity
To: sdattili@stormontvail.org, histonet@lists.utsouthwestern.edu
Date: Tuesday, September 27, 2011, 11:11 AM



Shelley,

Perhaps this employee should look into other job opportunities.? The histo lab is filled with chemicals that can cause sensitivity in those people who may be prone to that.? Unfortunately, some of the main players in the histo lab (xylene & formalin) are often the culprit, but they are the best products in terms of how well they work, how universally they are used, how well their characteristics are known, etc...? I hate to sound like I'm not sympathetic, because I truly am, but I think it is a mistake to modify a histology lab's procedures to accomodate a new employee.

Just my opinion,

Glen Dawson? BS, HT(ASCP) & QIHC
Milwaukee, WI


> Date: Tue, 27 Sep 2011 08:27:19 -0500
> From: SDattili@stormontvail.org
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Xylene sensitivity
> 
> Hi all,
> I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper). This is her first job in a lab of any sort. We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc. Right now she is wearing a PAPR to work, which is obviously not a long-term solution. Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.
> 
> Does anyone have any experience with activated charcoal air cleaners? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour. It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment. Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings. We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors. I am open to any suggestions.
> 
> Thanks,
> 
> Shelley D'Attilio MT(ASCP)
> Manager, Chemistry, Cytology and Histology Dept. of Pathology and 
> Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas
> 
> 
> 
> 
> NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225.
> 
> ******************************************************************************************************************
> 
> The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation.
> 
> ******************************************************************************************************************
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
??? ???????? ?????? ??? ? _______________________________________________
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From Montina.VanMeter <@t> pbrc.edu  Tue Sep 27 10:56:37 2011
From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter)
Date: Tue Sep 27 10:56:45 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
References: 
	
Message-ID: 

Shelly,

Is your new employee a certified histotech?  Did she rotate through
clinical labs while in histo school?  I would think she would have
experienced this sensitivity during that time period as well.

Tina


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Glen
Dawson
Sent: Tuesday, September 27, 2011 10:11 AM
To: sdattili@stormontvail.org; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene sensitivity


Shelley,
 
Perhaps this employee should look into other job opportunities.  The
histo lab is filled with chemicals that can cause sensitivity in those
people who may be prone to that.  Unfortunately, some of the main
players in the histo lab (xylene & formalin) are often the culprit, but
they are the best products in terms of how well they work, how
universally they are used, how well their characteristics are known,
etc...  I hate to sound like I'm not sympathetic, because I truly am,
but I think it is a mistake to modify a histology lab's procedures to
accomodate a new employee.
 
Just my opinion,
 
Glen Dawson  BS, HT(ASCP) & QIHC
Milwaukee, WI
 

> Date: Tue, 27 Sep 2011 08:27:19 -0500
> From: SDattili@stormontvail.org
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Xylene sensitivity
> 
> Hi all,
> I have a new employee who is developing a scratchy, painful throat and
some difficulty breathing when exposed to xylene (for instance, when the
cover is raised on the coverslipper). This is her first job in a lab of
any sort. We are investigating all the usual culprits--air handling
system, hoods, allergies or virus unrelated to histology, etc. Right now
she is wearing a PAPR to work, which is obviously not a long-term
solution. Ultimately, I think we will conclude that this employee has a
sensitivity to xylene and possibly other chemicals in the histology lab,
as other employees are not complaining about symptoms related to
chemicals.
> 
> Does anyone have any experience with activated charcoal air cleaners?
I am looking at a portable unit that sits on casters and provides 4 air
exchanges per hour. It's not cheap at $1000, but well worth it if it
will provide relief for this employee and allow her to continue her
employment. Our lab is approximately 800-1000 sq. ft in size with 8 foot
ceilings. We have 1 standard bio-hood for processing cytology fluids and
2 wall-mounted "air suckers" above our processors. I am open to any
suggestions.
> 
> Thanks,
> 
> Shelley D'Attilio MT(ASCP)
> Manager, Chemistry, Cytology and Histology Dept. of Pathology and 
> Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas
> 
> 
> 
> 
> NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a
doctor accepting new patients. Call (785) 354-5225.
> 
>
************************************************************************
******************************************
> 
> The information transmitted in this e-mail and in any replies and
forwards are for the sole use of the above individual(s) or entities and
may contain proprietary, privileged and/or highly confidential
information. Any unauthorized dissemination, review, distribution or
copying of these communications is strictly prohibited. If this e-mail
has been transmitted to you in error, please notify and return the
original message to the sender immediately at the above listed address.
Thank you for your cooperation.
> 
>
************************************************************************
******************************************
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From JWeems <@t> sjha.org  Tue Sep 27 11:03:39 2011
From: JWeems <@t> sjha.org (Weems, Joyce)
Date: Tue Sep 27 11:03:45 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: <869848DDBB7C5D4896A569A38B814E690551947E@CVMMB01.cvm.tamu.edu>
References: 
	<1317137707.83978.YahooMailClassic@web65703.mail.ac4.yahoo.com>
	<869848DDBB7C5D4896A569A38B814E690551947E@CVMMB01.cvm.tamu.edu>
Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640827B74167@CHEXCMS10.one.ads.che.org>

If you don't use it, you can prevent someone from entering a clinical trial. Plain and simple..  


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Cross
Sent: Tuesday, September 27, 2011 11:49
To: Rene J Buesa; Histonet Listserv (E-mail); ShelleyD'Attilio; Loralee AMcMahon
Subject: RE: [Histonet] Xylene sensitivity

I have gone around and around with DAKO because they want to blame the fact that I use Pro-Par rather than xylene in my lab. I use it for  processing and de-paraffinizing and some of their people want to blame that on their Artisan Link Auto Stainer staining inconsistently in my lab. . . I like xylene, but it sure is rough on some people! 

Kelly Cross

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, September 27, 2011 10:35 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; Loralee AMcMahon
Subject: RE: [Histonet] Xylene sensitivity

Loralee:
Poor people?in your sister hospital! You are affecting their health. I would have approached a different way and never done that.
DAKO cannot dictate how I process my samples. I would have gotten involved my legal department to handle that. 
I would have requested the experimental data from DAKO. They obtained approval from FDA using their process. They submitted that process that unfortunately included using xylene but that in no way invalidates the Herpset Tests results.
Are you going to tell me that the results of your sister hospital were wrong while using something other than xylene??I would have interchanged pieces of tissue and evaluate the results.
I would have demonstrated that there were no differences and I say this because that is what I did when processing with mineral oil at my hospital. The whole process was validated and we also did Hercept test from DAKO.
Do they also require using ethanol? Does it matter if you use 2-propanol?
Why limiting the process to just about xylene and not about the dehydrating agent?
Do they also try to determine the lenght of the process?
I think that accepting DAKO protocol without challenging it and forcing your sister hospital to expose their employees to xylene was absolutely wrong!
Forgive me to say this, but you took the "easy way" instead of challenging the whole issue and demonstrate that their requirement from the technical point of view absolutely baseless.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:


From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 10:57 AM








The?Hercept Test from?Dako specifies??xylene in the breast tissue processing section.? The Er/Pr?Pharm DX kit may also, but I haven't check that out.?? I know this because we just fought with a sister hospital to get them to change their processors to xylene.? 
?
?

Loralee?McMahon,?HTL (ASCP)
Immunohistochemistry?Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
?


From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:54 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity








Loralee:
Please enlighten me! As far as I know FDA requirements extend to fixation time?and NBF is required by some kit FDA approved, but not to how the tissues are processed.
Even there are studies that show that xylene has extracting effects over some epitopes.
I know that you cannot argue with FDA, but you can always validate any changes in your procedure.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:


From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 9:40 AM


Rene, 

I understand the desire to remove Xylene from the lab completely.? But what if you are running FDA approved kits that are only FDA approved if the tissue is processed in Xylene? 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:33 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: Re: [Histonet] Xylene sensitivity

Shelley:
As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution.
Is like having a pain when you use your arm and immobilizing it to prevent the pain.
Your long term and complete solution is to eliminate xylene from your lab and I mean from ALL tasks using it now.
Under separate cover I am sending 2 publications explaining how to do that.
Ren? J.

--- On Tue, 9/27/11, D'Attilio, Shelley  wrote:


From: D'Attilio, Shelley 
Subject: [Histonet] Xylene sensitivity
To: "Histonet Listserv (E-mail)" 
Date: Tuesday, September 27, 2011, 9:27 AM


Hi all,
I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper).? This is her first job in a lab of any sort.? We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc.? Right now she is wearing a PAPR to work, which is obviously not a long-term solution.? Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour.? It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment.? Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings.? We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors.? I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?? Stormont-Vail's Health Connections can help you find a doctor accepting new patients.? Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.? Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.? If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.? Thank you for your cooperation.

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From ihcman2010 <@t> hotmail.com  Tue Sep 27 11:26:33 2011
From: ihcman2010 <@t> hotmail.com (Glen Dawson)
Date: Tue Sep 27 11:26:38 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: <1317138275.75985.YahooMailClassic@web65709.mail.ac4.yahoo.com>
References: ,
	<1317138275.75985.YahooMailClassic@web65709.mail.ac4.yahoo.com>
Message-ID: 


Rene',
 
I must respectfully ask: what happens after you modify your procedures for this new employee & 2 days after everything has been standardized/validated another employee begins employment, but exhibits sensitivity to the new xylene substitute?  I realize that getting another job NOW is not easy, but redoing standardized procedures over and over could drive a lab into the ground and ALL of the techs in that lab could be searching for another job.
 
It is a mistake to automatically assume that substitutes cannot be toxic or cause sensitivity themselves.
 
Glen D.
 
 
 



Date: Tue, 27 Sep 2011 08:44:35 -0700
From: rjbuesa@yahoo.com
Subject: RE: [Histonet] Xylene sensitivity
To: sdattili@stormontvail.org; histonet@lists.utsouthwestern.edu; ihcman2010@hotmail.com






Glen:
I respectfully disagree. Do you have any idea what would mean to that employee to try to get another job NOW?
Changing the procedure is what we (as histotechnique refers to) have been doing for ever.
Perhaps you do not remember other methods, but I do because I started working in this field in 1952.
Back then I processed tissues manually and used aniline oil to "clear", benzene  as ante medium and was very glad when I switched to white gasoline and later to xylene.
Do you know why xylene became so prevalent? Because in 1943 the AutioTechnicon was developed and they recommended xylene as the way to go.
That selection also was the choice of the VIP manufacturers, and we all started to change from the toxic benzene and aniline oil to the toxic xylene.
There are other ways of doing things and changing work or exposing to toxic chemicals is not the correct path, no matter how much we are "used to".
Ren? J.

--- On Tue, 9/27/11, Glen Dawson  wrote:


From: Glen Dawson 
Subject: RE: [Histonet] Xylene sensitivity
To: sdattili@stormontvail.org, histonet@lists.utsouthwestern.edu
Date: Tuesday, September 27, 2011, 11:11 AM



Shelley,

Perhaps this employee should look into other job opportunities.  The histo lab is filled with chemicals that can cause sensitivity in those people who may be prone to that.  Unfortunately, some of the main players in the histo lab (xylene & formalin) are often the culprit, but they are the best products in terms of how well they work, how universally they are used, how well their characteristics are known, etc...  I hate to sound like I'm not sympathetic, because I truly am, but I think it is a mistake to modify a histology lab's procedures to accomodate a new employee.

Just my opinion,

Glen Dawson  BS, HT(ASCP) & QIHC
Milwaukee, WI


> Date: Tue, 27 Sep 2011 08:27:19 -0500
> From: SDattili@stormontvail.org
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Xylene sensitivity
> 
> Hi all,
> I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper). This is her first job in a lab of any sort. We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc. Right now she is wearing a PAPR to work, which is obviously not a long-term solution. Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.
> 
> Does anyone have any experience with activated charcoal air cleaners? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour. It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment. Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings. We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors. I am open to any suggestions.
> 
> Thanks,
> 
> Shelley D'Attilio MT(ASCP)
> Manager, Chemistry, Cytology and Histology
> Dept. of Pathology and Laboratory Medicine
> Stormont-Vail HealthCare
> Topeka, Kansas
> 
> 
> 
> 
> NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225.
> 
> ******************************************************************************************************************
> 
> The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation.
> 
> ******************************************************************************************************************
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
                          _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From liz <@t> premierlab.com  Tue Sep 27 11:32:07 2011
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Tue Sep 27 11:33:30 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: <1317131688.12777.YahooMailClassic@web65703.mail.ac4.yahoo.com>
Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA0A63@SBS2K8.premierlab.local>

Rene

Rene

Loralee is correct in some aspects and you have addressed this already with mentioning validation.  This basically has to do with the internal validation that is required when you bring these kits into the laboratory.  When you modify a 510K cleared kit or FDA approved kit it becomes a LDT or Laboratory Developed Test there is significant additional validation required because by changing the process you have essentially increased the risk.  Additional validation is required as defined by CLIA.  The manufacturer of the kit has gone through a process that essentially has decreased the laboratory risk when then get a kit FDA cleared or approved, if the lab chooses to modify this kit they essentially have increased their risk significantly and are required to do additional validation, more than would be required if you used the kit as recommended.

I'm thinking that all that is required if you use a cleared or approved kit is verification and not validation, but I may be incorrect. Validation is a more intense process.  Patty from Phenopath or Beth Shepard from Ventana would be able to comment on this process also.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, September 27, 2011 7:55 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; Loralee AMcMahon
Subject: RE: [Histonet] Xylene sensitivity

Loralee:
Please enlighten me! As far as I know FDA requirements extend to fixation time and NBF is required by some kit FDA approved, but not to how the tissues are processed.
Even there are studies that show that xylene has extracting effects over some epitopes.
I know that you cannot argue with FDA, but you can always validate any changes in your procedure.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:


From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 9:40 AM


Rene,

I understand the desire to remove Xylene from the lab completely.  But what if you are running FDA approved kits that are only FDA approved if the tissue is processed in Xylene?

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:33 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: Re: [Histonet] Xylene sensitivity

Shelley:
As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution.
Is like having a pain when you use your arm and immobilizing it to prevent the pain.
Your long term and complete solution is to eliminate xylene from your lab and I mean from ALL tasks using it now.
Under separate cover I am sending 2 publications explaining how to do that.
Ren? J.

--- On Tue, 9/27/11, D'Attilio, Shelley  wrote:


From: D'Attilio, Shelley 
Subject: [Histonet] Xylene sensitivity
To: "Histonet Listserv (E-mail)" 
Date: Tuesday, September 27, 2011, 9:27 AM


Hi all,
I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper).  This is her first job in a lab of any sort.  We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc.  Right now she is wearing a PAPR to work, which is obviously not a long-term solution.  Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?  I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour.  It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment.  Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings.  We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors.  I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.  Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.  If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.  Thank you for your cooperation.

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From rjbuesa <@t> yahoo.com  Tue Sep 27 11:35:57 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Sep 27 11:36:05 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
Message-ID: <1317141357.32194.YahooMailClassic@web65712.mail.ac4.yahoo.com>

Glen:
Unfortunately for your reasoning, I substituted xylene with MINERAL OIL, the same mineral oil you can profusely use on your skin while applying a "sun blocker", or the same mineral oil that constitutes the baby oil sold in any drug store to be applied to the any baby's hypersensitive skin.
I am attaching under separate cover my original procedure and another more recent modification all validated as to their effectiveness.
Ah, by the way, mineral oil is also part of some softening stool medicaments as well!
Ren? J.


--- On Tue, 9/27/11, Glen Dawson  wrote:


From: Glen Dawson 
Subject: RE: [Histonet] Xylene sensitivity
To: rjbuesa@yahoo.com, histonet@lists.utsouthwestern.edu
Date: Tuesday, September 27, 2011, 12:26 PM





Rene',
?
I must respectfully ask: what happens after you modify your procedures?for this new employee & 2 days after everything has been standardized/validated another employee begins employment, but?exhibits sensitivity to the new?xylene substitute?? I realize that getting another job NOW is not easy, but redoing standardized procedures over and over could?drive a lab into the ground and ALL?of the techs in that lab could be searching for another job.
?
It is a mistake to automatically assume that substitutes cannot be toxic?or cause?sensitivity themselves.
?
Glen D.
?
?
?



Date: Tue, 27 Sep 2011 08:44:35 -0700
From: rjbuesa@yahoo.com
Subject: RE: [Histonet] Xylene sensitivity
To: sdattili@stormontvail.org; histonet@lists.utsouthwestern.edu; ihcman2010@hotmail.com






Glen:
I respectfully disagree. Do you have any idea what would mean to that employee to try to get another job NOW?
Changing the procedure is what we (as histotechnique refers to) have been doing for ever.
Perhaps you do not remember other methods, but I do because I started working in this?field in 1952.
Back then I processed tissues manually and used aniline oil to "clear", benzene? as ante medium and was very glad when I switched to white gasoline and later to xylene.
Do you know why xylene became so prevalent? Because in 1943 the AutioTechnicon was developed and they recommended xylene as the way to go.
That selection also was the choice of the VIP manufacturers, and we all started to change from the toxic benzene and aniline oil to the toxic xylene.
There are other ways of doing things and changing work or exposing to toxic chemicals is not the correct path, no matter how much we are "used to".
Ren? J.

--- On Tue, 9/27/11, Glen Dawson  wrote:


From: Glen Dawson 
Subject: RE: [Histonet] Xylene sensitivity
To: sdattili@stormontvail.org, histonet@lists.utsouthwestern.edu
Date: Tuesday, September 27, 2011, 11:11 AM



Shelley,

Perhaps this employee should look into other job opportunities.? The histo lab is filled with chemicals that can cause sensitivity in those people who may be prone to that.? Unfortunately, some of the main players in the histo lab (xylene & formalin) are often the culprit, but they are the best products in terms of how well they work, how universally they are used, how well their characteristics are known, etc...? I hate to sound like I'm not sympathetic, because I truly am, but I think it is a mistake to modify a histology lab's procedures to accomodate a new employee.

Just my opinion,

Glen Dawson? BS, HT(ASCP) & QIHC
Milwaukee, WI


> Date: Tue, 27 Sep 2011 08:27:19 -0500
> From: SDattili@stormontvail.org
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Xylene sensitivity
> 
> Hi all,
> I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper). This is her first job in a lab of any sort. We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc. Right now she is wearing a PAPR to work, which is obviously not a long-term solution. Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.
> 
> Does anyone have any experience with activated charcoal air cleaners? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour. It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment. Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings. We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors. I am open to any suggestions.
> 
> Thanks,
> 
> Shelley D'Attilio MT(ASCP)
> Manager, Chemistry, Cytology and Histology
> Dept. of Pathology and Laboratory Medicine
> Stormont-Vail HealthCare
> Topeka, Kansas
> 
> 
> 
> 
> NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225.
> 
> ******************************************************************************************************************
> 
> The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation.
> 
> ******************************************************************************************************************
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
??? ???????? ?????? ??? ? _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From KCross <@t> cvm.tamu.edu  Tue Sep 27 11:42:14 2011
From: KCross <@t> cvm.tamu.edu (Kelly Cross)
Date: Tue Sep 27 11:42:20 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
References: ,
	<1317138275.75985.YahooMailClassic@web65709.mail.ac4.yahoo.com>
	
Message-ID: <869848DDBB7C5D4896A569A38B814E690551951D@CVMMB01.cvm.tamu.edu>

Glen, 
I suppose it would all depend upon which xylene substitute one uses - if one switches to another that has a pungent odor such as the citrus based solvents - there could be problems with that as well. I have no scientific data to back this up nor have I researched it, but I would think if there wasn't a pungent odor involved the issues might not be as bad concerning sensitivity. 

I personally have used xylene, and some of the citrus based solvents as well as the Pro-par (which doesn't have much of an odor at all) and a lot of the issues I have had have disappeared since I've  been with Pro-par. Not advocating one product over another, but is seems the less noxious the better the environment seems to be with xylene substitutes - all personal experience. I apologize for not having documentation to send to you to back up my claims. 

Just an opinion - one of many! 
Kelly

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Glen Dawson
Sent: Tuesday, September 27, 2011 11:27 AM
To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene sensitivity


Rene',
 
I must respectfully ask: what happens after you modify your procedures for this new employee & 2 days after everything has been standardized/validated another employee begins employment, but exhibits sensitivity to the new xylene substitute?  I realize that getting another job NOW is not easy, but redoing standardized procedures over and over could drive a lab into the ground and ALL of the techs in that lab could be searching for another job.
 
It is a mistake to automatically assume that substitutes cannot be toxic or cause sensitivity themselves.
 
Glen D.
 
 
 



Date: Tue, 27 Sep 2011 08:44:35 -0700
From: rjbuesa@yahoo.com
Subject: RE: [Histonet] Xylene sensitivity
To: sdattili@stormontvail.org; histonet@lists.utsouthwestern.edu; ihcman2010@hotmail.com






Glen:
I respectfully disagree. Do you have any idea what would mean to that employee to try to get another job NOW?
Changing the procedure is what we (as histotechnique refers to) have been doing for ever.
Perhaps you do not remember other methods, but I do because I started working in this field in 1952.
Back then I processed tissues manually and used aniline oil to "clear", benzene  as ante medium and was very glad when I switched to white gasoline and later to xylene.
Do you know why xylene became so prevalent? Because in 1943 the AutioTechnicon was developed and they recommended xylene as the way to go.
That selection also was the choice of the VIP manufacturers, and we all started to change from the toxic benzene and aniline oil to the toxic xylene.
There are other ways of doing things and changing work or exposing to toxic chemicals is not the correct path, no matter how much we are "used to".
Ren? J.

--- On Tue, 9/27/11, Glen Dawson  wrote:


From: Glen Dawson 
Subject: RE: [Histonet] Xylene sensitivity
To: sdattili@stormontvail.org, histonet@lists.utsouthwestern.edu
Date: Tuesday, September 27, 2011, 11:11 AM



Shelley,

Perhaps this employee should look into other job opportunities.  The histo lab is filled with chemicals that can cause sensitivity in those people who may be prone to that.  Unfortunately, some of the main players in the histo lab (xylene & formalin) are often the culprit, but they are the best products in terms of how well they work, how universally they are used, how well their characteristics are known, etc...  I hate to sound like I'm not sympathetic, because I truly am, but I think it is a mistake to modify a histology lab's procedures to accomodate a new employee.

Just my opinion,

Glen Dawson  BS, HT(ASCP) & QIHC
Milwaukee, WI


> Date: Tue, 27 Sep 2011 08:27:19 -0500
> From: SDattili@stormontvail.org
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Xylene sensitivity
> 
> Hi all,
> I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper). This is her first job in a lab of any sort. We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc. Right now she is wearing a PAPR to work, which is obviously not a long-term solution. Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.
> 
> Does anyone have any experience with activated charcoal air cleaners? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour. It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment. Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings. We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors. I am open to any suggestions.
> 
> Thanks,
> 
> Shelley D'Attilio MT(ASCP)
> Manager, Chemistry, Cytology and Histology Dept. of Pathology and 
> Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas
> 
> 
> 
> 
> NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225.
> 
> ******************************************************************************************************************
> 
> The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation.
> 
> ******************************************************************************************************************
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
                          _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From tpodawiltz <@t> lrgh.org  Tue Sep 27 11:44:33 2011
From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas)
Date: Tue Sep 27 11:44:56 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
References: ,
	<1317138275.75985.YahooMailClassic@web65709.mail.ac4.yahoo.com>
	
Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DF21AAD2@LRGHEXVS1.practice.lrgh.org>

Sometimes a new employee is like the old canary miners took with them. They alert you to bad air. Sometimes, after you have used a product for a while your body has adjusted to it and you cease to notice it. 

We use xylene, the last time a substitute was looked at one of the techs had a reaction to it so we stayed with xylene. 
The lab is negative pressure, the air exchange rate is 52 times per hour and all the equipment that generates fumes is either in a large hood or directly vented into the fume system. We also test for xylene and formalin once a year which is a OSHA requirement and the main hoods are inspected and tested twice per year. 

We have done everything we can to keep our air fresh no matter what chemical or reagent we use. 

Bottom line: Not all people can work in a Histology lab plain and simple. If someone starts reactions to our reagents as per our guidelines they will be sent to Employee Health for review. 

Tom

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Glen Dawson
Sent: Tuesday, September 27, 2011 12:27 PM
To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene sensitivity


Rene',
 
I must respectfully ask: what happens after you modify your procedures for this new employee & 2 days after everything has been standardized/validated another employee begins employment, but exhibits sensitivity to the new xylene substitute?  I realize that getting another job NOW is not easy, but redoing standardized procedures over and over could drive a lab into the ground and ALL of the techs in that lab could be searching for another job.
 
It is a mistake to automatically assume that substitutes cannot be toxic or cause sensitivity themselves.
 
Glen D.
 
 
 



Date: Tue, 27 Sep 2011 08:44:35 -0700
From: rjbuesa@yahoo.com
Subject: RE: [Histonet] Xylene sensitivity
To: sdattili@stormontvail.org; histonet@lists.utsouthwestern.edu; ihcman2010@hotmail.com






Glen:
I respectfully disagree. Do you have any idea what would mean to that employee to try to get another job NOW?
Changing the procedure is what we (as histotechnique refers to) have been doing for ever.
Perhaps you do not remember other methods, but I do because I started working in this field in 1952.
Back then I processed tissues manually and used aniline oil to "clear", benzene  as ante medium and was very glad when I switched to white gasoline and later to xylene.
Do you know why xylene became so prevalent? Because in 1943 the AutioTechnicon was developed and they recommended xylene as the way to go.
That selection also was the choice of the VIP manufacturers, and we all started to change from the toxic benzene and aniline oil to the toxic xylene.
There are other ways of doing things and changing work or exposing to toxic chemicals is not the correct path, no matter how much we are "used to".
Ren? J.

--- On Tue, 9/27/11, Glen Dawson  wrote:


From: Glen Dawson 
Subject: RE: [Histonet] Xylene sensitivity
To: sdattili@stormontvail.org, histonet@lists.utsouthwestern.edu
Date: Tuesday, September 27, 2011, 11:11 AM



Shelley,

Perhaps this employee should look into other job opportunities.  The histo lab is filled with chemicals that can cause sensitivity in those people who may be prone to that.  Unfortunately, some of the main players in the histo lab (xylene & formalin) are often the culprit, but they are the best products in terms of how well they work, how universally they are used, how well their characteristics are known, etc...  I hate to sound like I'm not sympathetic, because I truly am, but I think it is a mistake to modify a histology lab's procedures to accomodate a new employee.

Just my opinion,

Glen Dawson  BS, HT(ASCP) & QIHC
Milwaukee, WI


> Date: Tue, 27 Sep 2011 08:27:19 -0500
> From: SDattili@stormontvail.org
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Xylene sensitivity
> 
> Hi all,
> I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper). This is her first job in a lab of any sort. We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc. Right now she is wearing a PAPR to work, which is obviously not a long-term solution. Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.
> 
> Does anyone have any experience with activated charcoal air cleaners? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour. It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment. Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings. We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors. I am open to any suggestions.
> 
> Thanks,
> 
> Shelley D'Attilio MT(ASCP)
> Manager, Chemistry, Cytology and Histology
> Dept. of Pathology and Laboratory Medicine
> Stormont-Vail HealthCare
> Topeka, Kansas
> 
> 
> 
> 
> NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225.
> 
> ******************************************************************************************************************
> 
> The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation.
> 
> ******************************************************************************************************************
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
                          _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.  If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare.


From jqb7 <@t> cdc.gov  Tue Sep 27 11:45:41 2011
From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID))
Date: Tue Sep 27 11:46:53 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: <869848DDBB7C5D4896A569A38B814E690551951D@CVMMB01.cvm.tamu.edu>
References: ,
	<1317138275.75985.YahooMailClassic@web65709.mail.ac4.yahoo.com>
	
	<869848DDBB7C5D4896A569A38B814E690551951D@CVMMB01.cvm.tamu.edu>
Message-ID: 

The subject of the odor itself is interesting.  I had a colleague years ago that tried Pro-par and couldn't use it because she said it smelled like bug spray and it just made her nauseous.  I noticed the odor but it didn't offend me nearly as much as it did her.

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly Cross
Sent: Tuesday, September 27, 2011 12:42 PM
To: Glen Dawson; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene sensitivity

Glen, 
I suppose it would all depend upon which xylene substitute one uses - if one switches to another that has a pungent odor such as the citrus based solvents - there could be problems with that as well. I have no scientific data to back this up nor have I researched it, but I would think if there wasn't a pungent odor involved the issues might not be as bad concerning sensitivity. 

I personally have used xylene, and some of the citrus based solvents as well as the Pro-par (which doesn't have much of an odor at all) and a lot of the issues I have had have disappeared since I've  been with Pro-par. Not advocating one product over another, but is seems the less noxious the better the environment seems to be with xylene substitutes - all personal experience. I apologize for not having documentation to send to you to back up my claims. 

Just an opinion - one of many! 
Kelly

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Glen Dawson
Sent: Tuesday, September 27, 2011 11:27 AM
To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene sensitivity


Rene',
 
I must respectfully ask: what happens after you modify your procedures for this new employee & 2 days after everything has been standardized/validated another employee begins employment, but exhibits sensitivity to the new xylene substitute?  I realize that getting another job NOW is not easy, but redoing standardized procedures over and over could drive a lab into the ground and ALL of the techs in that lab could be searching for another job.
 
It is a mistake to automatically assume that substitutes cannot be toxic or cause sensitivity themselves.
 
Glen D.
 
 
 



Date: Tue, 27 Sep 2011 08:44:35 -0700
From: rjbuesa@yahoo.com
Subject: RE: [Histonet] Xylene sensitivity
To: sdattili@stormontvail.org; histonet@lists.utsouthwestern.edu; ihcman2010@hotmail.com






Glen:
I respectfully disagree. Do you have any idea what would mean to that employee to try to get another job NOW?
Changing the procedure is what we (as histotechnique refers to) have been doing for ever.
Perhaps you do not remember other methods, but I do because I started working in this field in 1952.
Back then I processed tissues manually and used aniline oil to "clear", benzene  as ante medium and was very glad when I switched to white gasoline and later to xylene.
Do you know why xylene became so prevalent? Because in 1943 the AutioTechnicon was developed and they recommended xylene as the way to go.
That selection also was the choice of the VIP manufacturers, and we all started to change from the toxic benzene and aniline oil to the toxic xylene.
There are other ways of doing things and changing work or exposing to toxic chemicals is not the correct path, no matter how much we are "used to".
Ren? J.

--- On Tue, 9/27/11, Glen Dawson  wrote:


From: Glen Dawson 
Subject: RE: [Histonet] Xylene sensitivity
To: sdattili@stormontvail.org, histonet@lists.utsouthwestern.edu
Date: Tuesday, September 27, 2011, 11:11 AM



Shelley,

Perhaps this employee should look into other job opportunities.  The histo lab is filled with chemicals that can cause sensitivity in those people who may be prone to that.  Unfortunately, some of the main players in the histo lab (xylene & formalin) are often the culprit, but they are the best products in terms of how well they work, how universally they are used, how well their characteristics are known, etc...  I hate to sound like I'm not sympathetic, because I truly am, but I think it is a mistake to modify a histology lab's procedures to accomodate a new employee.

Just my opinion,

Glen Dawson  BS, HT(ASCP) & QIHC
Milwaukee, WI


> Date: Tue, 27 Sep 2011 08:27:19 -0500
> From: SDattili@stormontvail.org
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Xylene sensitivity
> 
> Hi all,
> I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper). This is her first job in a lab of any sort. We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc. Right now she is wearing a PAPR to work, which is obviously not a long-term solution. Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.
> 
> Does anyone have any experience with activated charcoal air cleaners? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour. It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment. Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings. We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors. I am open to any suggestions.
> 
> Thanks,
> 
> Shelley D'Attilio MT(ASCP)
> Manager, Chemistry, Cytology and Histology Dept. of Pathology and 
> Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas
> 
> 
> 
> 
> NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225.
> 
> ******************************************************************************************************************
> 
> The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation.
> 
> ******************************************************************************************************************
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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From Loralee_Mcmahon <@t> URMC.Rochester.edu  Tue Sep 27 12:09:50 2011
From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A)
Date: Tue Sep 27 12:09:57 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: <1317137707.83978.YahooMailClassic@web65703.mail.ac4.yahoo.com>
References: ,
	<1317137707.83978.YahooMailClassic@web65703.mail.ac4.yahoo.com>
Message-ID: 

Well actually yes.  We do a trend analysis and have proven that according to the accepted rates their Her2 positivity was declining (went from 14%- 6%).  Meaning that some patients did not get the correct therapy.  I would have to say poor patient!!

And maybe it wasn't the xylene but if I was a lawyer and I got ahold of the data sheet and found out that my client didn't get herceptin because the hospital decided it would be better to not expose their employees to a chemical that if used handled properly is perfectly fine to use......and then found out that the hospital running the test on those tissue knew that they were not using xylene....as you can see it can get out of hand.

I am sure that there are arguments for both sides.  The Data from Dako was using thousands of cases.  I cannot process that many cases differently to validate it my way.   I am simply using the guidelines that they have established.    Maybe five or so years down the road there will be another company out there that can validate there antibody without using xyelene and get it FDA approved.  But they haven't yet.

And my boss is on the ASCOCAP taskforce, so I really can't argue with him.

Using Xylene on one processor that is ventilated and enclosed is really not exposing every employee to xylene.  If they use it properly they exposure rate is very small.  I would worry more about formalin or DAB.


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

________________________________
From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 11:35 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity

Loralee:
Poor people in your sister hospital! You are affecting their health. I would have approached a different way and never done that.
DAKO cannot dictate how I process my samples. I would have gotten involved my legal department to handle that.
I would have requested the experimental data from DAKO. They obtained approval from FDA using their process. They submitted that process that unfortunately included using xylene but that in no way invalidates the Herpset Tests results.
Are you going to tell me that the results of your sister hospital were wrong while using something other than xylene? I would have interchanged pieces of tissue and evaluate the results.
I would have demonstrated that there were no differences and I say this because that is what I did when processing with mineral oil at my hospital. The whole process was validated and we also did Hercept test from DAKO.
Do they also require using ethanol? Does it matter if you use 2-propanol?
Why limiting the process to just about xylene and not about the dehydrating agent?
Do they also try to determine the lenght of the process?
I think that accepting DAKO protocol without challenging it and forcing your sister hospital to expose their employees to xylene was absolutely wrong!
Forgive me to say this, but you took the "easy way" instead of challenging the whole issue and demonstrate that their requirement from the technical point of view absolutely baseless.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:

From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 10:57 AM

The Hercept Test from Dako specifies  xylene in the breast tissue processing section.  The Er/Pr Pharm DX kit may also, but I haven't check that out.   I know this because we just fought with a sister hospital to get them to change their processors to xylene.


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

________________________________
From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:54 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity

Loralee:
Please enlighten me! As far as I know FDA requirements extend to fixation time and NBF is required by some kit FDA approved, but not to how the tissues are processed.
Even there are studies that show that xylene has extracting effects over some epitopes.
I know that you cannot argue with FDA, but you can always validate any changes in your procedure.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:

From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 9:40 AM

Rene,

I understand the desire to remove Xylene from the lab completely.  But what if you are running FDA approved kits that are only FDA approved if the tissue is processed in Xylene?

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:33 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: Re: [Histonet] Xylene sensitivity

Shelley:
As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution.
Is like having a pain when you use your arm and immobilizing it to prevent the pain.
Your long term and complete solution is to eliminate xylene from your lab and I mean from ALL tasks using it now.
Under separate cover I am sending 2 publications explaining how to do that.
Ren? J.

--- On Tue, 9/27/11, D'Attilio, Shelley > wrote:


From: D'Attilio, Shelley >
Subject: [Histonet] Xylene sensitivity
To: "Histonet Listserv (E-mail)" >
Date: Tuesday, September 27, 2011, 9:27 AM


Hi all,
I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper).  This is her first job in a lab of any sort.  We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc.  Right now she is wearing a PAPR to work, which is obviously not a long-term solution.  Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?  I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour.  It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment.  Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings.  We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors.  I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.  Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.  If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.  Thank you for your cooperation.

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From rsrichmond <@t> gmail.com  Tue Sep 27 12:15:09 2011
From: rsrichmond <@t> gmail.com (Bob Richmond)
Date: Tue Sep 27 12:15:13 2011
Subject: [Histonet] Re: Xylene sensitivity
Message-ID: 

Some points that haven't been mentioned in this discussion:

I think most labs have switched from xylenes to aliphatic hydrocarbons
for processing, hydration, and dehydration though obviously
considerable controversy remains. (I can't imagine anybody switching
from xylene to limonene now - particularly because limonene smells bad
and is something of an allergen or other sensitizer. The aliphatics
(Pro-par and many others) are all quite odorless to my not very
sensitive nose.

The one place where you can't eliminate xylenes (or the closely
related aromatic hydrocarbons benzene and toluene) is in coverslipping
- they still haven't come up with an aromatics-free mounting resin.

For somebody with sensitivity problems, formaldehyde is just as big a
problem. Many clinical labs still don't have adequate ventilation of
their grossing areas - I'm working in two these days that don't - and
I wind up choking on the stuff fairly often. There is no substitute
for a gross desk that draws the air over your work into louvers set at
hand level - the Nut-One kitchen hood set high overhead doesn't do it.

It seems to me that a technologist with the problems you describe
ought to be able to work in a properly ventilated laboratory, and
since inadequate ventilation is an OSHA issue, histology labs really
need to be adequately ventilated.

Bob Richmond
Samurai Pathologist
Knoxville TN

From aaperghis <@t> uspath.com  Tue Sep 27 12:57:47 2011
From: aaperghis <@t> uspath.com (Adrienne Aperghis Kavanagh)
Date: Tue Sep 27 12:58:23 2011
Subject: [Histonet] IHC H.P. Negative Control
In-Reply-To: <12823886.222925.1317064185836.JavaMail.root@mail3d.brinkster.com>
Message-ID: <20139929.229728.1317146267935.JavaMail.root@mail3d.brinkster.com>

Hi Histonetters,

We would like to start to run up to 30 H.Pylori on our Benchmark daily.  Do GI labs that run H.P. on ALL stomach biopsies run a negative control for each patient tissue for H.P.?  I am just curious, as we have a 24 hour TAT, but the Benchmark only holds 30 slides.  What's everyone doing?

Thanks!

From nicole <@t> dlcjax.com  Tue Sep 27 13:24:59 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Tue Sep 27 13:30:55 2011
Subject: [Histonet] Facebook
In-Reply-To: <2100.208.62.167.196.1317136909.squirrel@webmail.realpages.com>
References: <2100.208.62.167.196.1317136909.squirrel@webmail.realpages.com>
Message-ID: <2550.208.62.167.196.1317147899.squirrel@webmail.realpages.com>

https://www.facebook.com/#!/pages/Histo-Ville/114622425272649

Here is the link for those of you who need it.



 Hello Histonetters,
>
>
> Just drooping in to say thanks to all those Who have joined Histo-ville on
> facebook. We are forming an awesome community and hope more will join.
> Stop by and like us.
>
> Nicole Tatum, HT ASCP
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



From srodriguez <@t> phenopath.com  Tue Sep 27 13:53:05 2011
From: srodriguez <@t> phenopath.com (Stephanie Rodriguez)
Date: Tue Sep 27 13:53:17 2011
Subject: [Histonet] Xylene/substitutes & Validation/verification
Message-ID: 

Liz,

You are correct. If you bring in an FDA-approved test with NO
modifications, all that is required is verification; you simply have to
verify that the test works in your lab when you follow the recommended
procedure.  Validation, which is required for laboratory-developed tests
as well as laboratory-modified FDA-approved tests, is, indeed, a much
lengthier process.

Stephanie Rodriguez, HTL(ASCP), QIHC
Lead Molecular Technologist-FISH
IHC Technologist III
Phenopath Laboratories
Seattle, WA
(206) 374-9000 



On 9/27/11 9:49 AM, "histonet-request@lists.utsouthwestern.edu"
 wrote:

>Message: 8
>Date: Tue, 27 Sep 2011 10:32:07 -0600
>From: Elizabeth Chlipala 
>Subject: RE: [Histonet] Xylene sensitivity
>To: 'Rene J Buesa' , "Histonet Listserv (E-mail)"
>	, ShelleyD'Attilio
>	, Loralee AMcMahon
>	
>Message-ID:
>	<14E2C6176416974295479C64A11CB9AE1DECBA0A63@SBS2K8.premierlab.local>
>Content-Type: text/plain; charset="iso-8859-1"
>
>Rene
>
>Rene
>
>Loralee is correct in some aspects and you have addressed this already
>with mentioning validation.  This basically has to do with the internal
>validation that is required when you bring these kits into the
>laboratory.  When you modify a 510K cleared kit or FDA approved kit it
>becomes a LDT or Laboratory Developed Test there is significant
>additional validation required because by changing the process you have
>essentially increased the risk.  Additional validation is required as
>defined by CLIA.  The manufacturer of the kit has gone through a process
>that essentially has decreased the laboratory risk when then get a kit
>FDA cleared or approved, if the lab chooses to modify this kit they
>essentially have increased their risk significantly and are required to
>do additional validation, more than would be required if you used the kit
>as recommended.
>
>I'm thinking that all that is required if you use a cleared or approved
>kit is verification and not validation, but I may be incorrect.
>Validation is a more intense process.  Patty from Phenopath or Beth
>Shepard from Ventana would be able to comment on this process also.
>
>Liz
>
>Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
>Manager
>Premier Laboratory, LLC
>PO Box 18592
>Boulder, CO 80308-1592
>(303) 682-3949 office
>(303) 682-9060 fax
>(303) 881-0763 cell
>www.premierlab.com
>
>Ship to address:
>
>1567 Skyway Drive, Unit E
>Longmont, CO 80504




This e-mail message, including any attachments, is for the sole use of the 
intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.


From andrea.conard <@t> gmail.com  Tue Sep 27 13:58:08 2011
From: andrea.conard <@t> gmail.com (andrea conard)
Date: Tue Sep 27 13:58:15 2011
Subject: [Histonet] histology workload benchmark
Message-ID: 

Hi histonetters,
I'm conducting a small benchmark study regarding workload distribution and
times slides get to pathologists, etc. We are NJ healthsystem with 2
hospitals but only do histo in one. We do 14k cases/16k blocks/year, 500
IHC/month and some special stains.

If you are a hospital or institution comparable to ours please send me info
about the questions below.


How many cases/how many blocks, case mix?



How many histo techs, Pas, lab assistants, pathologists.



When do HTs start, what time do the first slides get out/last slides get
out.



If more than one campus are pathologists stationed at all campuses or only
go there for frozens.



Is there a histo lab at all campuses or are specimens couriered to the main
facility.



How long is the histo lab/ gross room open?



What other functions do the histotechs do? Accessioning, send out cases,
filing, morgue, charging, clerical?



I really appreciate any help and info I can get with this project.

*Andrea Conard, HT(ASCP)*

*Pathology Supervisor*

*AtlantiCare** Regional Medical Center***

*Phone: 609-441-8168*

*Fax:609-441-2195*

You can emaill to andrea.conard@atlanticare.org
From rjbuesa <@t> yahoo.com  Tue Sep 27 14:42:53 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Sep 27 14:42:58 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
Message-ID: <1317152573.64640.YahooMailClassic@web65715.mail.ac4.yahoo.com>

You are assuming that the decline in your "sister" lab was due to not using xylene and you should never assume.
I would have taken several cases of mine, divided in two parts and given your "sister" lab half, and viceversa with some of their cases.
Compare both results and try to determine what was happening before taking such a drastic "solution" as making them to return to xylene.
I really have little patient with those that do not take good care of the staff.
It is the duty of us?supervisors and managers to take care of our staff. To provide them with? a safe and well regulated?working environment, protect them from some abusive pathologists and managers,?assure they are compensated properly and just making sure that in return the comply with all the performance standards.
I would never ever hastily expose my staff to an unsafe environment.
Ren? J.??

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:


From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 1:09 PM








Well actually yes.? We do a trend analysis and have proven that according to the accepted rates their?Her2 positivity was declining (went from 14%- 6%).? Meaning that some patients did not get the correct therapy.? I would have to say poor patient!!?? 
?
And maybe it wasn't the?xylene but if I was a lawyer and I got?ahold of the data sheet and found out that my client didn't get?herceptin because the hospital decided it would be better to not expose their employees to a chemical that if used handled properly is perfectly fine to use......and then found out that the hospital running the test on those tissue knew that they were not using xylene....as you can see it can get out of hand. 
?
I am sure that there are arguments for both sides.? The Data from?Dako was using thousands of cases.? I cannot process that many cases differently to validate it my way.?? I am simply using the guidelines that they have established.??? Maybe five or so years down the road there will be another company out there that can validate there antibody without using?xyelene and get it FDA approved.? But they haven't yet. 
?
And my boss is on the?ASCOCAP taskforce, so I really can't argue with him. 
?
Using?Xylene on one processor that is ventilated and enclosed is really not exposing every employee to xylene.? If they use it properly they exposure rate is very small.? I would worry more about formalin or DAB.? 
?
?

Loralee?McMahon,?HTL (ASCP)
Immunohistochemistry?Supervisor
Strong Memorial Hospital 
Department of Surgical Pathology
(585) 275-7210
?


From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 11:35 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity








Loralee:
Poor people?in your sister hospital! You are affecting their health. I would have approached a different way and never done that.
DAKO cannot dictate how I process my samples. I would have gotten involved my legal department to handle that. 
I would have requested the experimental data from DAKO. They obtained approval from FDA using their process. They submitted that process that unfortunately included using xylene but that in no way invalidates the Herpset Tests results.
Are you going to tell me that the results of your sister hospital were wrong while using something other than xylene??I would have interchanged pieces of tissue and evaluate the results.
I would have demonstrated that there were no differences and I say this because that is what I did when processing with mineral oil at my hospital. The whole process was validated and we also did Hercept test from DAKO.
Do they also require using ethanol? Does it matter if you use 2-propanol?
Why limiting the process to just about xylene and not about the dehydrating agent?
Do they also try to determine the lenght of the process?
I think that accepting DAKO protocol without challenging it and forcing your sister hospital to expose their employees to xylene was absolutely wrong!
Forgive me to say this, but you took the "easy way" instead of challenging the whole issue and demonstrate that their requirement from the technical point of view absolutely baseless.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:


From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 10:57 AM





#yiv465166059  P {
MARGIN-TOP:0px;MARGIN-BOTTOM:0px;}



The?Hercept Test from?Dako specifies??xylene in the breast tissue processing section.? The Er/Pr?Pharm DX kit may also, but I haven't check that out.?? I know this because we just fought with a sister hospital to get them to change their processors to xylene.? 
?
?

Loralee?McMahon,?HTL (ASCP)
Immunohistochemistry?Supervisor
Strong Memorial Hospital 
Department of Surgical Pathology
(585) 275-7210
?


From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:54 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity








Loralee:
Please enlighten me! As far as I know FDA requirements extend to fixation time?and NBF is required by some kit FDA approved, but not to how the tissues are processed.
Even there are studies that show that xylene has extracting effects over some epitopes.
I know that you cannot argue with FDA, but you can always validate any changes in your procedure.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:


From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 9:40 AM


Rene, 

I understand the desire to remove Xylene from the lab completely.? But what if you are running FDA approved kits that are only FDA approved if the tissue is processed in Xylene? 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:33 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: Re: [Histonet] Xylene sensitivity

Shelley:
As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution.
Is like having a pain when you use your arm and immobilizing it to prevent the pain.
Your long term and complete solution is to eliminate xylene from your lab and I mean from ALL tasks using it now.
Under separate cover I am sending 2 publications explaining how to do that.
Ren? J.

--- On Tue, 9/27/11, D'Attilio, Shelley  wrote:


From: D'Attilio, Shelley 
Subject: [Histonet] Xylene sensitivity
To: "Histonet Listserv (E-mail)" 
Date: Tuesday, September 27, 2011, 9:27 AM


Hi all,
I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper).? This is her first job in a lab of any sort.? We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc.? Right now she is wearing a PAPR to work, which is obviously not a long-term solution.? Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour.? It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment.? Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings.? We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors.? I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?? Stormont-Vail's Health Connections can help you find a doctor accepting new patients.? Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.? Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.? If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.? Thank you for your cooperation.

******************************************************************************************************************
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From SDattili <@t> stormontvail.org  Tue Sep 27 14:55:54 2011
From: SDattili <@t> stormontvail.org (D'Attilio, Shelley)
Date: Tue Sep 27 14:56:00 2011
Subject: [Histonet] RE: xylene sensitivity
In-Reply-To: <110E7925E2B91945A9B79EDFD0DC2B34E88D62E926@MCWMBX2.mcwcorp.net>
Message-ID: 

Kathryn,
Thanks for the advice.  I will evaluate the installation of a hood.  
 
Regards,
Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas 
  

-----Original Message-----
From: Stoll, Kathryn [mailto:kstoll@mcw.edu]
Sent: Tuesday, September 27, 2011 12:33 PM
To: D'Attilio, Shelley
Subject: xylene sensitivity



Shelley,

 

I also have the same xylene sensitivity.  In a previous job we coverslipped by hand.  I wore a N95 respirator mask when I was doing coverslipping.   

 

We eventually moved to a new building and the coverslipping was done under a hood.  If you could get a hood over the coverslipper or keep it closed that really helps.  (The same can be said for the stainer)

-          It takes cooperation from the other people you work with to comply.

 

I would also suggest to have this person cutting as far away from the xylene sources as possible.

 

Definitely the less exposure the better.  That seems to work the best for me.  But keep a fair balance between all duties and have them wear a mask as needed.  It is better than coughing all day and night.  

 

Good Luck.

Kathryn Stoll, HT(ASCP)

Depatment of Pathology

Medical College of Wisconsin

9200 W Wisconsin Ave

Milwaukee WI 53226

414.805.1525

kstoll@mcw.edu

 



NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.  Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.  If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.  Thank you for your cooperation.

******************************************************************************************************************
From rjbuesa <@t> yahoo.com  Tue Sep 27 14:56:45 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Sep 27 14:56:49 2011
Subject: [Histonet] histology workload benchmark
In-Reply-To: 
Message-ID: <1317153405.40524.YahooMailClassic@web65702.mail.ac4.yahoo.com>

Under separate cover I am sending you an article I wrote on the subject.
Just find in the benchmarks the column corresponding to the "lab class" you belong to and you will find the information you need.
Ren? J.

--- On Tue, 9/27/11, andrea conard  wrote:


From: andrea conard 
Subject: [Histonet] histology workload benchmark
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 27, 2011, 2:58 PM


Hi histonetters,
I'm conducting a small benchmark study regarding workload distribution and
times slides get to pathologists, etc. We are NJ healthsystem with 2
hospitals but only do histo in one. We do 14k cases/16k blocks/year, 500
IHC/month and some special stains.

If you are a hospital or institution comparable to ours please send me info
about the questions below.


How many cases/how many blocks, case mix?



How many histo techs, Pas, lab assistants, pathologists.



When do HTs start, what time do the first slides get out/last slides get
out.



If more than one campus are pathologists stationed at all campuses or only
go there for frozens.



Is there a histo lab at all campuses or are specimens couriered to the main
facility.



How long is the histo lab/ gross room open?



What other functions do the histotechs do? Accessioning, send out cases,
filing, morgue, charging, clerical?



I really appreciate any help and info I can get with this project.

*Andrea Conard, HT(ASCP)*

*Pathology Supervisor*

*AtlantiCare** Regional Medical Center***

*Phone: 609-441-8168*

*Fax:609-441-2195*

You can emaill to andrea.conard@atlanticare.org
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From Loralee_Mcmahon <@t> URMC.Rochester.edu  Tue Sep 27 14:59:09 2011
From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A)
Date: Tue Sep 27 14:59:14 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: <1317152573.64640.YahooMailClassic@web65715.mail.ac4.yahoo.com>
References: ,
	<1317152573.64640.YahooMailClassic@web65715.mail.ac4.yahoo.com>
Message-ID: 

We did not assume anything.
My sister hospital does not perform any immunos, we do all the immunos for them.  So that means the only variable is the processing of the tissue.  So where would you have started......?

Renee I really take offense to the fact that you just told me that I don't take my employee's health into consideration.  That is ridiculous of you to say that, not knowing the situation or the duration of time that we have spent on trying to fix this problem.  We did not do anything hastily.

Those tech are highly  trained to use xylene in the safest way possible among other chemicals in the lab.  There are milliions of unsafe things in the lab and hospital - radiation, chemo drugs, carcinogens, biohazards.   If techncians are giving the proper PPE and training they are very capable of using all types of chemicals and dealing with all types of biohazards.

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

________________________________
From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 3:42 PM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity

You are assuming that the decline in your "sister" lab was due to not using xylene and you should never assume.
I would have taken several cases of mine, divided in two parts and given your "sister" lab half, and viceversa with some of their cases.
Compare both results and try to determine what was happening before taking such a drastic "solution" as making them to return to xylene.
I really have little patient with those that do not take good care of the staff.
It is the duty of us supervisors and managers to take care of our staff. To provide them with  a safe and well regulated working environment, protect them from some abusive pathologists and managers, assure they are compensated properly and just making sure that in return the comply with all the performance standards.
I would never ever hastily expose my staff to an unsafe environment.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:

From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 1:09 PM

Well actually yes.  We do a trend analysis and have proven that according to the accepted rates their Her2 positivity was declining (went from 14%- 6%).  Meaning that some patients did not get the correct therapy.  I would have to say poor patient!!

And maybe it wasn't the xylene but if I was a lawyer and I got ahold of the data sheet and found out that my client didn't get herceptin because the hospital decided it would be better to not expose their employees to a chemical that if used handled properly is perfectly fine to use......and then found out that the hospital running the test on those tissue knew that they were not using xylene....as you can see it can get out of hand.

I am sure that there are arguments for both sides.  The Data from Dako was using thousands of cases.  I cannot process that many cases differently to validate it my way.   I am simply using the guidelines that they have established.    Maybe five or so years down the road there will be another company out there that can validate there antibody without using xyelene and get it FDA approved.  But they haven't yet.

And my boss is on the ASCOCAP taskforce, so I really can't argue with him.

Using Xylene on one processor that is ventilated and enclosed is really not exposing every employee to xylene.  If they use it properly they exposure rate is very small.  I would worry more about formalin or DAB.


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

________________________________
From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 11:35 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity

Loralee:
Poor people in your sister hospital! You are affecting their health. I would have approached a different way and never done that.
DAKO cannot dictate how I process my samples. I would have gotten involved my legal department to handle that.
I would have requested the experimental data from DAKO. They obtained approval from FDA using their process. They submitted that process that unfortunately included using xylene but that in no way invalidates the Herpset Tests results.
Are you going to tell me that the results of your sister hospital were wrong while using something other than xylene? I would have interchanged pieces of tissue and evaluate the results.
I would have demonstrated that there were no differences and I say this because that is what I did when processing with mineral oil at my hospital. The whole process was validated and we also did Hercept test from DAKO.
Do they also require using ethanol? Does it matter if you use 2-propanol?
Why limiting the process to just about xylene and not about the dehydrating agent?
Do they also try to determine the lenght of the process?
I think that accepting DAKO protocol without challenging it and forcing your sister hospital to expose their employees to xylene was absolutely wrong!
Forgive me to say this, but you took the "easy way" instead of challenging the whole issue and demonstrate that their requirement from the technical point of view absolutely baseless.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:

From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 10:57 AM

The Hercept Test from Dako specifies  xylene in the breast tissue processing section.  The Er/Pr Pharm DX kit may also, but I haven't check that out.   I know this because we just fought with a sister hospital to get them to change their processors to xylene.


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

________________________________
From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:54 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity

Loralee:
Please enlighten me! As far as I know FDA requirements extend to fixation time and NBF is required by some kit FDA approved, but not to how the tissues are processed.
Even there are studies that show that xylene has extracting effects over some epitopes.
I know that you cannot argue with FDA, but you can always validate any changes in your procedure.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:

From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 9:40 AM

Rene,

I understand the desire to remove Xylene from the lab completely.  But what if you are running FDA approved kits that are only FDA approved if the tissue is processed in Xylene?

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:33 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: Re: [Histonet] Xylene sensitivity

Shelley:
As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution.
Is like having a pain when you use your arm and immobilizing it to prevent the pain.
Your long term and complete solution is to eliminate xylene from your lab and I mean from ALL tasks using it now.
Under separate cover I am sending 2 publications explaining how to do that.
Ren? J.

--- On Tue, 9/27/11, D'Attilio, Shelley > wrote:


From: D'Attilio, Shelley >
Subject: [Histonet] Xylene sensitivity
To: "Histonet Listserv (E-mail)" >
Date: Tuesday, September 27, 2011, 9:27 AM


Hi all,
I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper).  This is her first job in a lab of any sort.  We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc.  Right now she is wearing a PAPR to work, which is obviously not a long-term solution.  Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?  I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour.  It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment.  Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings.  We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors.  I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.  Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.  If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.  Thank you for your cooperation.

******************************************************************************************************************
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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Histonet@lists.utsouthwestern.edu
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From sdysart <@t> mirnarx.com  Tue Sep 27 15:10:41 2011
From: sdysart <@t> mirnarx.com (Sarah Dysart)
Date: Tue Sep 27 15:12:32 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
References: ,
	<1317152573.64640.YahooMailClassic@web65715.mail.ac4.yahoo.com>
	
Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D507363@SN2PRD0702MB110.namprd07.prod.outlook.com>

Can ya'll take this argument off-line?  Obviously xylene is staying in Loralee's lab and Rene thinks Loralee is killing her employees.  We all get it now.  No real beneficial information is coming out of your argument that everyone needs to continue to read.
It's almost happy hour time...margaritas for all!!!
=)

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A
Sent: Tuesday, September 27, 2011 2:59 PM
To: Rene J Buesa; Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: RE: [Histonet] Xylene sensitivity

We did not assume anything.
My sister hospital does not perform any immunos, we do all the immunos for them.  So that means the only variable is the processing of the tissue.  So where would you have started......?

Renee I really take offense to the fact that you just told me that I don't take my employee's health into consideration.  That is ridiculous of you to say that, not knowing the situation or the duration of time that we have spent on trying to fix this problem.  We did not do anything hastily.

Those tech are highly  trained to use xylene in the safest way possible among other chemicals in the lab.  There are milliions of unsafe things in the lab and hospital - radiation, chemo drugs, carcinogens, biohazards.   If techncians are giving the proper PPE and training they are very capable of using all types of chemicals and dealing with all types of biohazards.

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

________________________________
From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 3:42 PM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity

You are assuming that the decline in your "sister" lab was due to not using xylene and you should never assume.
I would have taken several cases of mine, divided in two parts and given your "sister" lab half, and viceversa with some of their cases.
Compare both results and try to determine what was happening before taking such a drastic "solution" as making them to return to xylene.
I really have little patient with those that do not take good care of the staff.
It is the duty of us supervisors and managers to take care of our staff. To provide them with  a safe and well regulated working environment, protect them from some abusive pathologists and managers, assure they are compensated properly and just making sure that in return the comply with all the performance standards.
I would never ever hastily expose my staff to an unsafe environment.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:

From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 1:09 PM

Well actually yes.  We do a trend analysis and have proven that according to the accepted rates their Her2 positivity was declining (went from 14%- 6%).  Meaning that some patients did not get the correct therapy.  I would have to say poor patient!!

And maybe it wasn't the xylene but if I was a lawyer and I got ahold of the data sheet and found out that my client didn't get herceptin because the hospital decided it would be better to not expose their employees to a chemical that if used handled properly is perfectly fine to use......and then found out that the hospital running the test on those tissue knew that they were not using xylene....as you can see it can get out of hand.

I am sure that there are arguments for both sides.  The Data from Dako was using thousands of cases.  I cannot process that many cases differently to validate it my way.   I am simply using the guidelines that they have established.    Maybe five or so years down the road there will be another company out there that can validate there antibody without using xyelene and get it FDA approved.  But they haven't yet.

And my boss is on the ASCOCAP taskforce, so I really can't argue with him.

Using Xylene on one processor that is ventilated and enclosed is really not exposing every employee to xylene.  If they use it properly they exposure rate is very small.  I would worry more about formalin or DAB.


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

________________________________
From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 11:35 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity

Loralee:
Poor people in your sister hospital! You are affecting their health. I would have approached a different way and never done that.
DAKO cannot dictate how I process my samples. I would have gotten involved my legal department to handle that.
I would have requested the experimental data from DAKO. They obtained approval from FDA using their process. They submitted that process that unfortunately included using xylene but that in no way invalidates the Herpset Tests results.
Are you going to tell me that the results of your sister hospital were wrong while using something other than xylene? I would have interchanged pieces of tissue and evaluate the results.
I would have demonstrated that there were no differences and I say this because that is what I did when processing with mineral oil at my hospital. The whole process was validated and we also did Hercept test from DAKO.
Do they also require using ethanol? Does it matter if you use 2-propanol?
Why limiting the process to just about xylene and not about the dehydrating agent?
Do they also try to determine the lenght of the process?
I think that accepting DAKO protocol without challenging it and forcing your sister hospital to expose their employees to xylene was absolutely wrong!
Forgive me to say this, but you took the "easy way" instead of challenging the whole issue and demonstrate that their requirement from the technical point of view absolutely baseless.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:

From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 10:57 AM

The Hercept Test from Dako specifies  xylene in the breast tissue processing section.  The Er/Pr Pharm DX kit may also, but I haven't check that out.   I know this because we just fought with a sister hospital to get them to change their processors to xylene.


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

________________________________
From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:54 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity

Loralee:
Please enlighten me! As far as I know FDA requirements extend to fixation time and NBF is required by some kit FDA approved, but not to how the tissues are processed.
Even there are studies that show that xylene has extracting effects over some epitopes.
I know that you cannot argue with FDA, but you can always validate any changes in your procedure.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:

From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 9:40 AM

Rene,

I understand the desire to remove Xylene from the lab completely.  But what if you are running FDA approved kits that are only FDA approved if the tissue is processed in Xylene?

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:33 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: Re: [Histonet] Xylene sensitivity

Shelley:
As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution.
Is like having a pain when you use your arm and immobilizing it to prevent the pain.
Your long term and complete solution is to eliminate xylene from your lab and I mean from ALL tasks using it now.
Under separate cover I am sending 2 publications explaining how to do that.
Ren? J.

--- On Tue, 9/27/11, D'Attilio, Shelley > wrote:


From: D'Attilio, Shelley >
Subject: [Histonet] Xylene sensitivity
To: "Histonet Listserv (E-mail)" >
Date: Tuesday, September 27, 2011, 9:27 AM


Hi all,
I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper).  This is her first job in a lab of any sort.  We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc.  Right now she is wearing a PAPR to work, which is obviously not a long-term solution.  Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?  I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour.  It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment.  Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings.  We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors.  I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.  Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.  If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.  Thank you for your cooperation.

******************************************************************************************************************
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
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From MHillmer <@t> dermwisconsin.com  Tue Sep 27 15:22:14 2011
From: MHillmer <@t> dermwisconsin.com (Michael Hillmer)
Date: Tue Sep 27 15:22:18 2011
Subject: [Histonet] Immediate Job Opening-Manitowoc
Message-ID: 


We are looking for a certified HT or HTL, or applicants eligible to sit for the certification exam, to join our pathology lab in beautiful, downtown, Manitowoc, WI.  We currently have a fantastic 2nd  shift position open.  We have excellent benefits ( 13% employer money contributed to retirement plans), state-of-the-art lab (less than a year old), with views of Lake Michigan from its many windows and a great team to work with.  We will assist with relocation.  Please contact me if you have any questio s or to set up an interview.

Michael Hillmer PHR
HR Coordinator
Dermatology Associates of Wisconsin
Phone: (920)683-5278
Fax: (920)686-9674
Cell: (920)860-6360

The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule.  If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law.  If you have received this e-mail in error, please notify me by reply e-mail and then delete this message.  Do not pass any of this information to anyone else.  Thank you for your cooperation.

________________________________

The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation.
From SDattili <@t> stormontvail.org  Tue Sep 27 15:24:03 2011
From: SDattili <@t> stormontvail.org (D'Attilio, Shelley)
Date: Tue Sep 27 15:24:08 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
Message-ID: 

Hi Sarah,
Thanks so much for the information.  We do have a small fume extractor next to our formalin containers in the Gross Room.  This might be worth a try for the coverslipper anyway.  I believe that the piece of equipment I am thinking of buying while we evaluate xylene replacements is a larger version of that type of device, so it might work quite well.

Thanks for taking the time to respond,
Shelley

-----Original Message-----
From: Sarah Holmes [mailto:sarah@kidneybiopsy.com]
Sent: Tuesday, September 27, 2011 12:22 PM
To: D'Attilio, Shelley
Subject: Re: [Histonet] Xylene sensitivity


Not fully understanding your setup, we have had great success with 
inexpensive local fume extractors, like welders use, at our grossing and 
coverslipping sites.  We still hand coverslip in front of a container of 
xylene (holding roughly 400ml) and we set the fume extractor right behind it 
to divert all fumes thru the charcoal filter.  The fan competes so strongly 
with airflow that xylene (formalin) fumes are never in the breathing zone of 
the worker, and they are run thru an activated charcoal filter which we 
change every 3 months.

Available at technitool.com, item number 272SO350 is the extractor, 272SO352 
are filters.

Hope this helps!

Sarah Holmes
Laboratory Manager
Laboratory for Kidney Pathology, Inc.
1916 Patterson St, Suite 501
Nashville, TN 37203
615-321-5729






----- Original Message ----- 
From: "D'Attilio, Shelley" 
To: "Histonet Listserv (E-mail)" 
Sent: Tuesday, September 27, 2011 8:27 AM
Subject: [Histonet] Xylene sensitivity


Hi all,
I have a new employee who is developing a scratchy, painful throat and some 
difficulty breathing when exposed to xylene (for instance, when the cover is 
raised on the coverslipper).  This is her first job in a lab of any sort. 
We are investigating all the usual culprits--air handling system, hoods, 
allergies or virus unrelated to histology, etc.  Right now she is wearing a 
PAPR to work, which is obviously not a long-term solution.  Ultimately, I 
think we will conclude that this employee has a sensitivity to xylene and 
possibly other chemicals in the histology lab, as other employees are not 
complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?  I am 
looking at a portable unit that sits on casters and provides 4 air exchanges 
per hour.  It's not cheap at $1000, but well worth it if it will provide 
relief for this employee and allow her to continue her employment.  Our lab 
is approximately 800-1000 sq. ft in size with 8 foot ceilings.  We have 1 
standard bio-hood for processing cytology fluids and 2 wall-mounted "air 
suckers" above our processors.  I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a 
doctor accepting new patients.  Call (785) 354-5225.

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proprietary, privileged and/or highly confidential information.  Any 
unauthorized dissemination, review, distribution or copying of these 
communications is strictly prohibited.  If this e-mail has been transmitted 
to you in error, please notify and return the original message to the sender 
immediately at the above listed address.  Thank you for your cooperation.

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From MHillmer <@t> dermwisconsin.com  Tue Sep 27 15:41:05 2011
From: MHillmer <@t> dermwisconsin.com (Michael Hillmer)
Date: Tue Sep 27 15:41:10 2011
Subject: [Histonet] Wages
Message-ID: 

Does anyone know of resources for find accurate wage information for histology personnel in a clinic setting (particularly in Wisconsin)?

Thanks, any help would be greatly appreciated.

Michael Hillmer PHR
HR Coordinator
Dermatology Associates of Wisconsin
Phone: (920)683-5278
Fax: (920)663-9004
Cell: (920)860-6360
mhillmer@dermwisconsin.com
.
________________________________

The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation.
From Loralee_Mcmahon <@t> URMC.Rochester.edu  Tue Sep 27 15:46:45 2011
From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A)
Date: Tue Sep 27 15:47:04 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D507363@SN2PRD0702MB110.namprd07.prod.outlook.com>
References: ,
	<1317152573.64640.YahooMailClassic@web65715.mail.ac4.yahoo.com>
	,
	<8A70A9B2ECDD084DACFE6C59FCF86D507363@SN2PRD0702MB110.namprd07.prod.outlook.com>
Message-ID: 

I agree.  Sorry.......

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: Sarah Dysart [sdysart@mirnarx.com]
Sent: Tuesday, September 27, 2011 4:10 PM
To: McMahon, Loralee A; Rene J Buesa; Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: RE: [Histonet] Xylene sensitivity

Can ya'll take this argument off-line?  Obviously xylene is staying in Loralee's lab and Rene thinks Loralee is killing her employees.  We all get it now.  No real beneficial information is coming out of your argument that everyone needs to continue to read.
It's almost happy hour time...margaritas for all!!!
=)

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A
Sent: Tuesday, September 27, 2011 2:59 PM
To: Rene J Buesa; Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: RE: [Histonet] Xylene sensitivity

We did not assume anything.
My sister hospital does not perform any immunos, we do all the immunos for them.  So that means the only variable is the processing of the tissue.  So where would you have started......?

Renee I really take offense to the fact that you just told me that I don't take my employee's health into consideration.  That is ridiculous of you to say that, not knowing the situation or the duration of time that we have spent on trying to fix this problem.  We did not do anything hastily.

Those tech are highly  trained to use xylene in the safest way possible among other chemicals in the lab.  There are milliions of unsafe things in the lab and hospital - radiation, chemo drugs, carcinogens, biohazards.   If techncians are giving the proper PPE and training they are very capable of using all types of chemicals and dealing with all types of biohazards.

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

________________________________
From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 3:42 PM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity

You are assuming that the decline in your "sister" lab was due to not using xylene and you should never assume.
I would have taken several cases of mine, divided in two parts and given your "sister" lab half, and viceversa with some of their cases.
Compare both results and try to determine what was happening before taking such a drastic "solution" as making them to return to xylene.
I really have little patient with those that do not take good care of the staff.
It is the duty of us supervisors and managers to take care of our staff. To provide them with  a safe and well regulated working environment, protect them from some abusive pathologists and managers, assure they are compensated properly and just making sure that in return the comply with all the performance standards.
I would never ever hastily expose my staff to an unsafe environment.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:

From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 1:09 PM

Well actually yes.  We do a trend analysis and have proven that according to the accepted rates their Her2 positivity was declining (went from 14%- 6%).  Meaning that some patients did not get the correct therapy.  I would have to say poor patient!!

And maybe it wasn't the xylene but if I was a lawyer and I got ahold of the data sheet and found out that my client didn't get herceptin because the hospital decided it would be better to not expose their employees to a chemical that if used handled properly is perfectly fine to use......and then found out that the hospital running the test on those tissue knew that they were not using xylene....as you can see it can get out of hand.

I am sure that there are arguments for both sides.  The Data from Dako was using thousands of cases.  I cannot process that many cases differently to validate it my way.   I am simply using the guidelines that they have established.    Maybe five or so years down the road there will be another company out there that can validate there antibody without using xyelene and get it FDA approved.  But they haven't yet.

And my boss is on the ASCOCAP taskforce, so I really can't argue with him.

Using Xylene on one processor that is ventilated and enclosed is really not exposing every employee to xylene.  If they use it properly they exposure rate is very small.  I would worry more about formalin or DAB.


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

________________________________
From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 11:35 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity

Loralee:
Poor people in your sister hospital! You are affecting their health. I would have approached a different way and never done that.
DAKO cannot dictate how I process my samples. I would have gotten involved my legal department to handle that.
I would have requested the experimental data from DAKO. They obtained approval from FDA using their process. They submitted that process that unfortunately included using xylene but that in no way invalidates the Herpset Tests results.
Are you going to tell me that the results of your sister hospital were wrong while using something other than xylene? I would have interchanged pieces of tissue and evaluate the results.
I would have demonstrated that there were no differences and I say this because that is what I did when processing with mineral oil at my hospital. The whole process was validated and we also did Hercept test from DAKO.
Do they also require using ethanol? Does it matter if you use 2-propanol?
Why limiting the process to just about xylene and not about the dehydrating agent?
Do they also try to determine the lenght of the process?
I think that accepting DAKO protocol without challenging it and forcing your sister hospital to expose their employees to xylene was absolutely wrong!
Forgive me to say this, but you took the "easy way" instead of challenging the whole issue and demonstrate that their requirement from the technical point of view absolutely baseless.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:

From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 10:57 AM

The Hercept Test from Dako specifies  xylene in the breast tissue processing section.  The Er/Pr Pharm DX kit may also, but I haven't check that out.   I know this because we just fought with a sister hospital to get them to change their processors to xylene.


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

________________________________
From: Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:54 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A
Subject: RE: [Histonet] Xylene sensitivity

Loralee:
Please enlighten me! As far as I know FDA requirements extend to fixation time and NBF is required by some kit FDA approved, but not to how the tissues are processed.
Even there are studies that show that xylene has extracting effects over some epitopes.
I know that you cannot argue with FDA, but you can always validate any changes in your procedure.
Ren? J.

--- On Tue, 9/27/11, McMahon, Loralee A  wrote:

From: McMahon, Loralee A 
Subject: RE: [Histonet] Xylene sensitivity
To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" 
Date: Tuesday, September 27, 2011, 9:40 AM

Rene,

I understand the desire to remove Xylene from the lab completely.  But what if you are running FDA approved kits that are only FDA approved if the tissue is processed in Xylene?

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com]
Sent: Tuesday, September 27, 2011 9:33 AM
To: Histonet Listserv (E-mail); ShelleyD'Attilio
Subject: Re: [Histonet] Xylene sensitivity

Shelley:
As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution.
Is like having a pain when you use your arm and immobilizing it to prevent the pain.
Your long term and complete solution is to eliminate xylene from your lab and I mean from ALL tasks using it now.
Under separate cover I am sending 2 publications explaining how to do that.
Ren? J.

--- On Tue, 9/27/11, D'Attilio, Shelley > wrote:


From: D'Attilio, Shelley >
Subject: [Histonet] Xylene sensitivity
To: "Histonet Listserv (E-mail)" >
Date: Tuesday, September 27, 2011, 9:27 AM


Hi all,
I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper).  This is her first job in a lab of any sort.  We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc.  Right now she is wearing a PAPR to work, which is obviously not a long-term solution.  Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?  I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour.  It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment.  Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings.  We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors.  I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

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From liz <@t> premierlab.com  Tue Sep 27 15:55:54 2011
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Tue Sep 27 15:57:17 2011
Subject: [Histonet] Feulgan and Rossenbeck
Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA0A88@SBS2K8.premierlab.local>

Hello everyone

Does anyone out there know if you can purchase this reagent, from my searches it looks like a modified Schiff reagent.  When someone is requesting this stain as the Feulgan and Rossenbeck is it just the Feulgan stain?  Sorry this question seems really basic but to be honest I'm a bit stumped over it.  Thanks in advance.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

From SDattili <@t> stormontvail.org  Tue Sep 27 16:02:39 2011
From: SDattili <@t> stormontvail.org (D'Attilio, Shelley)
Date: Tue Sep 27 16:02:43 2011
Subject: [Histonet] RE: Xylene sensitivity
In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DF21AAB0@LRGHEXVS1.practice.lrgh.org>
Message-ID: 

Hi Tom,
Yes, our Employee Health & Safety department is involved with this problem.  Our lab is negative pressure, but do not know the air exchange rate.  I will definitely ask that to be checked.  

We monitor employees annually and had a full environmental assessment 2-3 years ago prior to installation of a new air handling system for the histology lab.

The coverslipper and stainer are not in a hood.  The Leica H&E Auto Stainer is under two large "air suckers" that are installed in the walls (along with our Peloris and two old Shandon tissue processors), and the coverslipper is on a bench.  Manual coverslipping is rarely performed, but we do have a standard chemical/bio hood for that if required.

A broken belt was discovered on the air handling system yesterday and was repaired.  Hopefully this will help a little bit.

Regards,
Shelley


NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.  Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.  If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.  Thank you for your cooperation.

******************************************************************************************************************

From SDattili <@t> stormontvail.org  Tue Sep 27 16:08:01 2011
From: SDattili <@t> stormontvail.org (D'Attilio, Shelley)
Date: Tue Sep 27 16:08:05 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
Message-ID: 

Hi Jan,
Thanks for the advice on xylene substitutes.  Your symptoms mirror those of my affected employee and I'm glad to hear that you found relief using another product.  I'm anxious to fix this situation as quickly as possible.  This employee is ready to make a career of Histology and I would like to see that happen if I can.

Regards,
Shelley


NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.  Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.  If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.  Thank you for your cooperation.

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From SDattili <@t> stormontvail.org  Tue Sep 27 16:13:48 2011
From: SDattili <@t> stormontvail.org (D'Attilio, Shelley)
Date: Tue Sep 27 16:13:52 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: <1317130416.25049.YahooMailClassic@web65710.mail.ac4.yahoo.com>
Message-ID: 

Rene',
Thanks for the quick response to my question.  The two articles that you sent were very interesting and I have forwarded them to my department histotech supervisor for her evaluation.  
 
It is apparent from your comments on the listserv that you have a strong commitment to safety.  You have given me much to think about and some new things to try.  I may still try the filter/hood option during evaluation of xylene-free options.
 
Regards,
Shelley

 



NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.  Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.  If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.  Thank you for your cooperation.

******************************************************************************************************************
From amosbrooks <@t> gmail.com  Tue Sep 27 16:14:52 2011
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Tue Sep 27 16:14:56 2011
Subject: [Histonet] Xylene sensitivity
Message-ID: 

Hi,
    I can attest to the effacacy of the isopropyl/mineral oil processing
that Rene describes. It works great for murine heart samples. I would not
switch entirely though, since xylene does a fine job with most other
purposes. My main concern is the rather hysterical impulse to get rid of
every chemical we use.
    We all work with chemicals in this line of work every day. It is just
part of the job. We need to avoid the hazards as best we can, but they are
there nonetheless. We can't remove all the chemicals and work with nothing
but distilled water. It just doesn't work like that. Hazardous chemicals can
be safely used without resorting to changing long standing procedures.
Picric Acid, osmium tetroxide, formalin and a multitude of other chemicals
are certainly able to be used as long as the user is aware of the risks and
uses proper protection.
    If someone develops a sensitivity to a chemical as common as xylene, it
is not unlikely that they will develop sensitivities to others as well. We
should attempt to see if they can reduce their exposure, but if everything
that can be reasonably done has been done, perhaps the best course would be
retraining into another lab would be best. This is not a cruel punishment.
It is a courteous way of assisting the person in evolving their career into
something more suited to their personal needs.

Chemistry happens,
Amos

On Tue, Sep 27, 2011 at 12:45 PM,  wrote:

> Message: 11
> Date: Tue, 27 Sep 2011 12:44:33 -0400
> From: "Podawiltz, Thomas" 
> Subject: RE: [Histonet] Xylene sensitivity
> To: Glen Dawson , "rjbuesa@yahoo.com"
>        , "histonet@lists.utsouthwestern.edu"
>        
> Message-ID:
>        <
> 38667E7FB77ECD4E91BFAEB8D986386323DF21AAD2@LRGHEXVS1.practice.lrgh.org>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Sometimes a new employee is like the old canary miners took with them. They
> alert you to bad air. Sometimes, after you have used a product for a while
> your body has adjusted to it and you cease to notice it.
>
> We use xylene, the last time a substitute was looked at one of the techs
> had a reaction to it so we stayed with xylene.
> The lab is negative pressure, the air exchange rate is 52 times per hour
> and all the equipment that generates fumes is either in a large hood or
> directly vented into the fume system. We also test for xylene and formalin
> once a year which is a OSHA requirement and the main hoods are inspected and
> tested twice per year.
>
> We have done everything we can to keep our air fresh no matter what
> chemical or reagent we use.
>
> Bottom line: Not all people can work in a Histology lab plain and simple.
> If someone starts reactions to our reagents as per our guidelines they will
> be sent to Employee Health for review.
>
> Tom
>
From KMB01 <@t> grh.org  Tue Sep 27 16:17:46 2011
From: KMB01 <@t> grh.org (Kathy M. Gorham)
Date: Tue Sep 27 16:17:50 2011
Subject: [Histonet] KP Markers
Message-ID: 

I received a call from Mercedes Medical saying they can not get the KP
Markers so many of us like.  Does any one have another source for these
wonderful pens?  Thanks and have a wonderful evening, Kathy Gorham H.T.


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From Timothy.Morken <@t> ucsfmedctr.org  Tue Sep 27 16:26:14 2011
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy)
Date: Tue Sep 27 16:26:21 2011
Subject: [Histonet] RE: Incredible job opportunity - Kansas City, MO
In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF985814027@EXCHMB-02.stowers-institute.org>
References: <2C40E43D1F7A56408C4463FD245DDDF985814027@EXCHMB-02.stowers-institute.org>
Message-ID: <8D7C2D242DBD45498006B21122072BF89448A23D@MCINFRWEM003.ucsfmedicalcenter.org>

>From my experience visiting Teri's lab I can attest that the Stower's institute is an astounding institution and a fantastic opportunity. Anyone interested in working in a truly top notch research environment that values its people should take her up on it immediately.

Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri
Sent: Monday, September 26, 2011 10:22 AM
To: Histonet
Subject: [Histonet] Incredible job opportunity - Kansas City, MO

I am reposting this in case it was missed earlier. The Stowers Institute is looking for someone with a solid background in Histology, including research histology and supervisory experience to head the Histology and Electron Microscopy Core Facility. I will post the actual ad below, but before I do just let me say that working at this facility is a once-in-a-lifetime experience. I urge you to visit the website, www.stowers.org, and read about the type of reseach they are doing here, and learn more about the Institute and their mission. If you have been considering a career move, take and look and see if this is something that interests you.

This is a challenging but incredibly rewarding position. You will learn something new every day and contribute to really great science. Yes, this is my position they are replacing. It has been a great 10 years here, and whomever takes over will be managing a staff of really talented histology and electron microscopy specialists. I am leaving due to personal reasons, it has nothing whatsoever to do with the management, staff, or any issue I have with the Institute. Trust me, it was very difficult to make the decision to leave. This is by far the best job I have ever had.

I will be moving onward and forward and will still be a regular contributor to the histonet after starting my new position next month at GNF (Genomics Institute for Novartis Foundation). I am looking forward to the challenge.

Teri Johnson, HT(ASCP)QIHC
Head, Histology and Electron Microscopy
Stowers Institute for Medical Research
Kansas City, MO


The Stowers Institute for Medical Research has an opening for a Head of Histology and Electron Microscopy to oversee expert delivery of the highest quality service for the detection of gene and protein expression in tissue samples; histochemical staining; sample fixation; routine histology; and ultra-structural analysis.

 Primary responsibilities include maintaining the current status of projects and resolving issues in the EM and Histology labs; actively promoting team interaction and participation; monitoring workload and turn-around time through the LIMS system; providing oversight and feedback on complex or non-routine projects; monitoring usage of services; responding appropriately to unexpected peaks in workload and service requests; maintaining effective communication with all members of the Institute; troubleshooting problems and communicating appropriately with Principal Investigators and the scientific staff, administration, and other core facility personnel as needed; making formal and informal presentations on Core Center services; ensuring continuing education of staff through workshops, webinars, lab meetings, and email communications; and reading professional journals and other sources to stay current in Histologic and EM techniques.

In addition to outstanding communication skills and the ability to effectively multi-task in a team-oriented environment, the successful candidate will have QIHC (ASCP) Qualification, and HT or HTL certification.  Previous management or supervisory experience is preferred.

Minimum requirements include an undergraduate degree in the sciences; excellent knowledge of histologic technique to include fixation, processing, histochemical staining, immunohistochemical staining, microtomy (to include cryostat, paraffin, and plastic), and ultramicrotomy; experience in a research environment with animal model histology; and the ability to apply good judgement to troubleshooting, problem solving, and staff management.  Five to ten years relevant Histology and/or Electron Microscopy experience in lieu of education may be considered.

To apply, send resume and coverletter to careers@stowers.org




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From laurie.colbert <@t> huntingtonhospital.com  Tue Sep 27 17:14:48 2011
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Tue Sep 27 17:14:52 2011
Subject: [Histonet] KP Markers
In-Reply-To: 
References: 
Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2B15A@EXCHANGE3.huntingtonhospital.com>

I received the same info.  If someone has another source, please let us
all know.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M.
Gorham
Sent: Tuesday, September 27, 2011 2:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] KP Markers

I received a call from Mercedes Medical saying they can not get the KP
Markers so many of us like.  Does any one have another source for these
wonderful pens?  Thanks and have a wonderful evening, Kathy Gorham H.T.


GRH National Recognition
Outstanding Rural Health Organization of 2009 awarded by NRHA
Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
Leader in Innovative Excellence 2009 awarded by the OAHHS
Financial Excellence Award 2010 awarded by the national Rural Health
Research & Policy Analysis Center
Healthcare Achievement Award for Quality in Patient Care Delivery and
Satisfaction 2010 awarded by Amerinet
Health Devices Achievement Award 2011 awarded by ECRI Institute

GRH Mission
We will ensure access to high-quality, cost-effective health services in
a safe, customer-friendly environment for all those in need of our
services.


GRH Confidentiality Notice
This e-mail and any attached documents are for the intended recipient/s
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From llewllew <@t> shaw.ca  Tue Sep 27 18:56:40 2011
From: llewllew <@t> shaw.ca (Bryan Llewellyn)
Date: Tue Sep 27 18:56:47 2011
Subject: [Histonet] Feulgan and Rossenbeck
In-Reply-To: <14E2C6176416974295479C64A11CB9AE1DECBA0A88@SBS2K8.premierlab.local>
References: <14E2C6176416974295479C64A11CB9AE1DECBA0A88@SBS2K8.premierlab.local>
Message-ID: <4E8262B8.3000006@shaw.ca>

Got to StainsFile at for details of Feulgen and Rossenbeck;s Schiff 
reagent.

http://stainsfile.info/StainsFile/stain/schiff/feulgenrossenbeck.htm

Bryan Llewellyn



Elizabeth Chlipala wrote:
> Hello everyone
>
> Does anyone out there know if you can purchase this reagent, from my searches it looks like a modified Schiff reagent.  When someone is requesting this stain as the Feulgan and Rossenbeck is it just the Feulgan stain?  Sorry this question seems really basic but to be honest I'm a bit stumped over it.  Thanks in advance.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308-1592
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> www.premierlab.com
>
> Ship to address:
>
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

From SteveM <@t> mcclainlab.com  Tue Sep 27 19:10:59 2011
From: SteveM <@t> mcclainlab.com (Steve McClain)
Date: Tue Sep 27 18:58:07 2011
Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 35 XYLENE
Message-ID: 

It seems there is a debate on three or more issues, 
one being the validation of a test where xylene was the processing standard 
another being the safety of xylene where an employee is allergic or sensitized to xylene,
a third on poor lab design and poor ventilation,
 another on the safety of xylene as a potential toxin.

>From a dermatology perspective, formaldehyde is a great risk as a sensitizer.
Latex gloves are a greater risk for allergy than xylene- latex allergies are common among persons with nut allergies.
Allergies to the Limonene oils were/are also common.
Xylene  may induce bronchospasm and I assume the employee under discussion has issues in this regard.

If an employee were to have an issue, I suppose I would re-train them and assign them to other duties or help them find another job. 

Apparently there are others whose emotions run high on this subject.
Xylene is toxic, yet may be handled with relative safety assuming adequate ventilation.
In the absence of adequate ventilation one should refuse to handle any volatile liquids at all and find a new job.
I fail to see what there is to be emotional about xylene.
Xylene can be detected by most ordinary human noses at about 0.01 to 1ppm, or 1/100th to 1/10,000th of the acceptable 8 hour exposure limits.
OSHA and the CDC have fairly well established standards for workplace exposure.
For the CDC information- see http://www.atsdr.cdc.gov/mmg/mmg.asp?id=291&tid=53

In my own lab we designed for sufficient air exchange so I do not smell any fumes.
However, we have 3000 cubic feet per minute fans pulling fumes out of the grossing and solvent areas of the lab including stainers, coverslippers, processors and grossing benches.
And we keep containers closed whenever possible.

Steve A. McClain, MD
631 361 4000

Here are part of the CDC  data published on that website.
Medical Management Guidelines for Xylene 
(C6H4)(CH3)2

 PDF Version, 47 KB


CAS#: 1330-20-7 
UN#: 1307

Synonyms include dimethylbenzene, methyl toluene, xylol, and mixed xylenes.

Persons exposed only to xylene vapor do not pose substantial risks of secondary contamination. Persons whose clothing or skin is contaminated with liquid xylene can cause secondary contamination by direct contact or through off-gassing vapor. 
Xylene is a clear, colorless liquid. It is volatile, readily producing flammable and toxic concentrations at room temperature. Its vapor is heavier than air and may accumulate in low-lying areas. Xylene's odor generally provides adequate warning of hazardous concentrations. 
Xylene is rapidly absorbed after inhalation and ingestion. Exposure via ingestion and inhalation produces systemic toxicity. Xylene is slowly absorbed through intact skin, but percutaneous absorption may contribute to total body burden. 
General Information 

Description

Xylene exists as three isomers (ortho-, meta-, and para-xylene), which can be found singly or, more commonly, mixed in varying proportions. Commercial grade xylene, in which m-xylene is usually the major constituent, is a clear, colorless liquid with a sweet, aromatic odor. It is generally referred to as Mixed, Total or Technical-Grade Xylene. There is a fourth structural isomer, ethylbenzene (C6H5)(C2H5) that may be present as a congener, but is not toxicologically distinct to a significant degree. Xylene is flammable at room temperature; therefore, it constitutes a fire hazard. It is insoluble in water, but mixes readily with many organic solvents. Xylene is less dense than water and will float on the surface of water.

Routes of Exposure

Inhalation

Most exposures to xylene occur by inhalation and xylene is readily absorbed from the lungs. Xylene's odor threshold is about 1 ppm, which is 100 times less than the OSHA PEL and generally provides adequate warning of acutely hazardous concentrations. Irritation of eye and throat occur at about 200 ppm. Xylene vapor is heavier than air and may cause asphyxiation in enclosed, poorly ventilated, or low-lying areas.

Children exposed to the same levels of xylene vapor as adults may receive a larger dose because they have greater lung surface area:body weight ratios and increased minute volumes:weight ratios. In addition, they may be exposed to higher levels than adults in the same location because of their short stature and the higher levels of xylene vapor found nearer to the ground. 

Skin/Eye Contact

Xylene vapor is only mildly irritating to mucous membranes; however, xylene splashed in the eyes can result in corneal injury. Repeated or prolonged skin contact with liquid xylene can defat the skin, causing it to crack and peel. Percutaneous absorption is slow through intact skin; however, xylene absorbed through the skin may contribute to body burden.

Children are more vulnerable to toxicants absorbed through the skin because of their relatively larger surface area:body weight ratio.

Ingestion

Acute systemic toxicity can result from ingestion of xylene.

Sources/Uses

Xylene is among the 30 most abundantly produced chemicals in the United States. It is obtained primarily from crude petroleum. It is widely used as a degreasing agent and as a thinner and solvent in paints, inks, adhesives, and many other products. It is commonly found as a solvent in pesticide products.

Standards and Guidelines

OSHA PEL (permissible exposure limit) = 100 ppm (averaged over an 8-hour workshift) NIOSH IDLH (immediately dangerous to life or health) = 900 ppm

Physical Properties 
Description: Clear, colorless liquid

Warning properties: Adequate; sweet, aromatic odor at 1 ppm 

Molecular weight: 106.2 daltons

Boiling point (760 mm Hg)*: 292?F (144?C), 269?F (139?C), and 281?F (138?C)

Freezing point*: -13?F (-25?C), -54?F (-48?C), and 56?F (13?C)

Specific gravity*: 0.88, 0.86, and 0.86 (water = 1)

Vapor pressure*: 5, 6, and 6.5 mm Hg at 68?F (20?C)

Gas density: 3.8 (air = 1)

Water solubility: insoluble

Flammability*: 63?F (17?C), 81?F (27?C), 81?F (27?C)

Flammable range: 1.0% to 7.0% (concentration in air)

*ortho-, meta-, and para-xylene, respectively.

Incompatibilities
Xylene reacts with strong oxidizers and strong acids.

top

--------------------------------------------------------------------------------

Health Effects 


Xylene is irritating to the skin, eyes, and respiratory tract. It can cause systemic toxicity by ingestion or inhalation. The most common route of exposure is via inhalation. 
Symptoms of xylene poisoning include CNS effects (headache, dizziness, ataxia, drowsiness, excitement, tremor, and coma), ventricular arrythmias, acute pulmonary edema, respiratory depression, nausea, vomiting, and reversible hepatic impairment. 
The mechanism by which xylene produces toxicity is not known... see website http://www.atsdr.cdc.gov/mmg/mmg.asp?id=291&tid=53

From joelleweaver <@t> hotmail.com  Tue Sep 27 20:46:47 2011
From: joelleweaver <@t> hotmail.com (joelle weaver )
Date: Tue Sep 27 20:46:51 2011
Subject: [Histonet] Wages
Message-ID: 

Have you already consulted the annual survey conducted by the ASCP? It is available on their website, and divides salaries by title, geographic region and facility size.
Joelle
Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: Michael Hillmer 
Date: Tue, 27 Sep 2011 20:41:05 
To: 
Subject: [Histonet] Wages

Does anyone know of resources for find accurate wage information for histology personnel in a clinic setting (particularly in Wisconsin)?

Thanks, any help would be greatly appreciated.

Michael Hillmer PHR
HR Coordinator
Dermatology Associates of Wisconsin
Phone: (920)683-5278
Fax: (920)663-9004
Cell: (920)860-6360
mhillmer@dermwisconsin.com
.
________________________________

The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation.
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From Loralee_Mcmahon <@t> URMC.Rochester.edu  Tue Sep 27 21:00:55 2011
From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A)
Date: Tue Sep 27 21:01:33 2011
Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 35 XYLENE
In-Reply-To: 
References: 
Message-ID: 

Thanks Steve,  
Now I don't feel as if I am trying to slowly kill my technician!!

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steve McClain [SteveM@mcclainlab.com]
Sent: Tuesday, September 27, 2011 8:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 35 XYLENE

It seems there is a debate on three or more issues,
one being the validation of a test where xylene was the processing standard
another being the safety of xylene where an employee is allergic or sensitized to xylene,
a third on poor lab design and poor ventilation,
 another on the safety of xylene as a potential toxin.

>From a dermatology perspective, formaldehyde is a great risk as a sensitizer.
Latex gloves are a greater risk for allergy than xylene- latex allergies are common among persons with nut allergies.
Allergies to the Limonene oils were/are also common.
Xylene  may induce bronchospasm and I assume the employee under discussion has issues in this regard.

If an employee were to have an issue, I suppose I would re-train them and assign them to other duties or help them find another job.

Apparently there are others whose emotions run high on this subject.
Xylene is toxic, yet may be handled with relative safety assuming adequate ventilation.
In the absence of adequate ventilation one should refuse to handle any volatile liquids at all and find a new job.
I fail to see what there is to be emotional about xylene.
Xylene can be detected by most ordinary human noses at about 0.01 to 1ppm, or 1/100th to 1/10,000th of the acceptable 8 hour exposure limits.
OSHA and the CDC have fairly well established standards for workplace exposure.
For the CDC information- see http://www.atsdr.cdc.gov/mmg/mmg.asp?id=291&tid=53

In my own lab we designed for sufficient air exchange so I do not smell any fumes.
However, we have 3000 cubic feet per minute fans pulling fumes out of the grossing and solvent areas of the lab including stainers, coverslippers, processors and grossing benches.
And we keep containers closed whenever possible.

Steve A. McClain, MD
631 361 4000

Here are part of the CDC  data published on that website.
Medical Management Guidelines for Xylene
(C6H4)(CH3)2

 PDF Version, 47 KB


CAS#: 1330-20-7
UN#: 1307

Synonyms include dimethylbenzene, methyl toluene, xylol, and mixed xylenes.

Persons exposed only to xylene vapor do not pose substantial risks of secondary contamination. Persons whose clothing or skin is contaminated with liquid xylene can cause secondary contamination by direct contact or through off-gassing vapor.
Xylene is a clear, colorless liquid. It is volatile, readily producing flammable and toxic concentrations at room temperature. Its vapor is heavier than air and may accumulate in low-lying areas. Xylene's odor generally provides adequate warning of hazardous concentrations.
Xylene is rapidly absorbed after inhalation and ingestion. Exposure via ingestion and inhalation produces systemic toxicity. Xylene is slowly absorbed through intact skin, but percutaneous absorption may contribute to total body burden.
General Information

Description

Xylene exists as three isomers (ortho-, meta-, and para-xylene), which can be found singly or, more commonly, mixed in varying proportions. Commercial grade xylene, in which m-xylene is usually the major constituent, is a clear, colorless liquid with a sweet, aromatic odor. It is generally referred to as Mixed, Total or Technical-Grade Xylene. There is a fourth structural isomer, ethylbenzene (C6H5)(C2H5) that may be present as a congener, but is not toxicologically distinct to a significant degree. Xylene is flammable at room temperature; therefore, it constitutes a fire hazard. It is insoluble in water, but mixes readily with many organic solvents. Xylene is less dense than water and will float on the surface of water.

Routes of Exposure

Inhalation

Most exposures to xylene occur by inhalation and xylene is readily absorbed from the lungs. Xylene's odor threshold is about 1 ppm, which is 100 times less than the OSHA PEL and generally provides adequate warning of acutely hazardous concentrations. Irritation of eye and throat occur at about 200 ppm. Xylene vapor is heavier than air and may cause asphyxiation in enclosed, poorly ventilated, or low-lying areas.

Children exposed to the same levels of xylene vapor as adults may receive a larger dose because they have greater lung surface area:body weight ratios and increased minute volumes:weight ratios. In addition, they may be exposed to higher levels than adults in the same location because of their short stature and the higher levels of xylene vapor found nearer to the ground.

Skin/Eye Contact

Xylene vapor is only mildly irritating to mucous membranes; however, xylene splashed in the eyes can result in corneal injury. Repeated or prolonged skin contact with liquid xylene can defat the skin, causing it to crack and peel. Percutaneous absorption is slow through intact skin; however, xylene absorbed through the skin may contribute to body burden.

Children are more vulnerable to toxicants absorbed through the skin because of their relatively larger surface area:body weight ratio.

Ingestion

Acute systemic toxicity can result from ingestion of xylene.

Sources/Uses

Xylene is among the 30 most abundantly produced chemicals in the United States. It is obtained primarily from crude petroleum. It is widely used as a degreasing agent and as a thinner and solvent in paints, inks, adhesives, and many other products. It is commonly found as a solvent in pesticide products.

Standards and Guidelines

OSHA PEL (permissible exposure limit) = 100 ppm (averaged over an 8-hour workshift) NIOSH IDLH (immediately dangerous to life or health) = 900 ppm

Physical Properties
Description: Clear, colorless liquid

Warning properties: Adequate; sweet, aromatic odor at 1 ppm

Molecular weight: 106.2 daltons

Boiling point (760 mm Hg)*: 292?F (144?C), 269?F (139?C), and 281?F (138?C)

Freezing point*: -13?F (-25?C), -54?F (-48?C), and 56?F (13?C)

Specific gravity*: 0.88, 0.86, and 0.86 (water = 1)

Vapor pressure*: 5, 6, and 6.5 mm Hg at 68?F (20?C)

Gas density: 3.8 (air = 1)

Water solubility: insoluble

Flammability*: 63?F (17?C), 81?F (27?C), 81?F (27?C)

Flammable range: 1.0% to 7.0% (concentration in air)

*ortho-, meta-, and para-xylene, respectively.

Incompatibilities
Xylene reacts with strong oxidizers and strong acids.

top

--------------------------------------------------------------------------------

Health Effects


Xylene is irritating to the skin, eyes, and respiratory tract. It can cause systemic toxicity by ingestion or inhalation. The most common route of exposure is via inhalation.
Symptoms of xylene poisoning include CNS effects (headache, dizziness, ataxia, drowsiness, excitement, tremor, and coma), ventricular arrythmias, acute pulmonary edema, respiratory depression, nausea, vomiting, and reversible hepatic impairment.
The mechanism by which xylene produces toxicity is not known... see website http://www.atsdr.cdc.gov/mmg/mmg.asp?id=291&tid=53

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From JMacDonald <@t> mtsac.edu  Tue Sep 27 22:35:01 2011
From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald)
Date: Tue Sep 27 22:35:09 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
Message-ID: 

I understand the reluctance to discontinue the use of the xylene in the 
tissue processor for FDA compliance, but why not change the solvent for 
staining and coverslipping?  Just curious.
Jennifer MacDonald





"D'Attilio, Shelley"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
09/27/2011 01:25 PM

To
"Sarah Holmes" 
cc
"Histonet Listserv \(E-mail\)" 
Subject
RE: [Histonet] Xylene sensitivity






Hi Sarah,
Thanks so much for the information.  We do have a small fume extractor 
next to our formalin containers in the Gross Room.  This might be worth a 
try for the coverslipper anyway.  I believe that the piece of equipment I 
am thinking of buying while we evaluate xylene replacements is a larger 
version of that type of device, so it might work quite well.

Thanks for taking the time to respond,
Shelley

-----Original Message-----
From: Sarah Holmes [mailto:sarah@kidneybiopsy.com]
Sent: Tuesday, September 27, 2011 12:22 PM
To: D'Attilio, Shelley
Subject: Re: [Histonet] Xylene sensitivity


Not fully understanding your setup, we have had great success with 
inexpensive local fume extractors, like welders use, at our grossing and 
coverslipping sites.  We still hand coverslip in front of a container of 
xylene (holding roughly 400ml) and we set the fume extractor right behind 
it 
to divert all fumes thru the charcoal filter.  The fan competes so 
strongly 
with airflow that xylene (formalin) fumes are never in the breathing zone 
of 
the worker, and they are run thru an activated charcoal filter which we 
change every 3 months.

Available at technitool.com, item number 272SO350 is the extractor, 
272SO352 
are filters.

Hope this helps!

Sarah Holmes
Laboratory Manager
Laboratory for Kidney Pathology, Inc.
1916 Patterson St, Suite 501
Nashville, TN 37203
615-321-5729






----- Original Message ----- 
From: "D'Attilio, Shelley" 
To: "Histonet Listserv (E-mail)" 
Sent: Tuesday, September 27, 2011 8:27 AM
Subject: [Histonet] Xylene sensitivity


Hi all,
I have a new employee who is developing a scratchy, painful throat and 
some 
difficulty breathing when exposed to xylene (for instance, when the cover 
is 
raised on the coverslipper).  This is her first job in a lab of any sort. 
We are investigating all the usual culprits--air handling system, hoods, 
allergies or virus unrelated to histology, etc.  Right now she is wearing 
a 
PAPR to work, which is obviously not a long-term solution.  Ultimately, I 
think we will conclude that this employee has a sensitivity to xylene and 
possibly other chemicals in the histology lab, as other employees are not 
complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?  I 
am 
looking at a portable unit that sits on casters and provides 4 air 
exchanges 
per hour.  It's not cheap at $1000, but well worth it if it will provide 
relief for this employee and allow her to continue her employment.  Our 
lab 
is approximately 800-1000 sq. ft in size with 8 foot ceilings.  We have 1 
standard bio-hood for processing cytology fluids and 2 wall-mounted "air 
suckers" above our processors.  I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a 
doctor accepting new patients.  Call (785) 354-5225.

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are for the sole use of the above individual(s) or entities and may 
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proprietary, privileged and/or highly confidential information.  Any 
unauthorized dissemination, review, distribution or copying of these 
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to you in error, please notify and return the original message to the 
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immediately at the above listed address.  Thank you for your cooperation.

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NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a 
doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards 
are for the sole use of the above individual(s) or entities and may 
contain proprietary, privileged and/or highly confidential information. 
Any unauthorized dissemination, review, distribution or copying of these 
communications is strictly prohibited.  If this e-mail has been 
transmitted to you in error, please notify and return the original message 
to the sender immediately at the above listed address.  Thank you for your 
cooperation.

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From H.J.G.vandeKant <@t> uu.nl  Wed Sep 28 00:32:26 2011
From: H.J.G.vandeKant <@t> uu.nl (Kant, H.J.G. van de (Henk))
Date: Wed Sep 28 00:32:48 2011
Subject: [Histonet] Toluidine blue
Message-ID: <8A014D954ADDDD44AC39749D8EB677C9A981BC@ICTSC-W-S205.soliscom.uu.nl>

Hi All,

Have someone a good workong protocol for staining mastcells in cytospin slides with Toluidine Blue.
Mention also the pH and fixation.

Thanks,

Henk van de Kant
Utrecht University, Faculty of Science, Division of Pharmacology,
David de Wied Building, Universiteitsweg 99, 3584 CG, Utrecht
The Netherlands
From Susan.Walzer <@t> HCAHealthcare.com  Wed Sep 28 02:05:28 2011
From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com)
Date: Wed Sep 28 02:05:36 2011
Subject: [Histonet] Xylene sensitivity
In-Reply-To: 
References: 
	
Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DD8B6F342@FWDCWPMSGCMS09.hca.corpad.net>

If you use Clearium mounting media from Surgipath you can coverslip out of isopropyl alcohol.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald
Sent: Tuesday, September 27, 2011 11:35 PM
To: D'Attilio, Shelley
Cc: Histonet Listserv (E-mail); histonet-bounces@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene sensitivity

I understand the reluctance to discontinue the use of the xylene in the 
tissue processor for FDA compliance, but why not change the solvent for 
staining and coverslipping?  Just curious.
Jennifer MacDonald





"D'Attilio, Shelley"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
09/27/2011 01:25 PM

To
"Sarah Holmes" 
cc
"Histonet Listserv \(E-mail\)" 
Subject
RE: [Histonet] Xylene sensitivity






Hi Sarah,
Thanks so much for the information.  We do have a small fume extractor 
next to our formalin containers in the Gross Room.  This might be worth a 
try for the coverslipper anyway.  I believe that the piece of equipment I 
am thinking of buying while we evaluate xylene replacements is a larger 
version of that type of device, so it might work quite well.

Thanks for taking the time to respond,
Shelley

-----Original Message-----
From: Sarah Holmes [mailto:sarah@kidneybiopsy.com]
Sent: Tuesday, September 27, 2011 12:22 PM
To: D'Attilio, Shelley
Subject: Re: [Histonet] Xylene sensitivity


Not fully understanding your setup, we have had great success with 
inexpensive local fume extractors, like welders use, at our grossing and 
coverslipping sites.  We still hand coverslip in front of a container of 
xylene (holding roughly 400ml) and we set the fume extractor right behind 
it 
to divert all fumes thru the charcoal filter.  The fan competes so 
strongly 
with airflow that xylene (formalin) fumes are never in the breathing zone 
of 
the worker, and they are run thru an activated charcoal filter which we 
change every 3 months.

Available at technitool.com, item number 272SO350 is the extractor, 
272SO352 
are filters.

Hope this helps!

Sarah Holmes
Laboratory Manager
Laboratory for Kidney Pathology, Inc.
1916 Patterson St, Suite 501
Nashville, TN 37203
615-321-5729






----- Original Message ----- 
From: "D'Attilio, Shelley" 
To: "Histonet Listserv (E-mail)" 
Sent: Tuesday, September 27, 2011 8:27 AM
Subject: [Histonet] Xylene sensitivity


Hi all,
I have a new employee who is developing a scratchy, painful throat and 
some 
difficulty breathing when exposed to xylene (for instance, when the cover 
is 
raised on the coverslipper).  This is her first job in a lab of any sort. 
We are investigating all the usual culprits--air handling system, hoods, 
allergies or virus unrelated to histology, etc.  Right now she is wearing 
a 
PAPR to work, which is obviously not a long-term solution.  Ultimately, I 
think we will conclude that this employee has a sensitivity to xylene and 
possibly other chemicals in the histology lab, as other employees are not 
complaining about symptoms related to chemicals.

Does anyone have any experience with activated charcoal air cleaners?  I 
am 
looking at a portable unit that sits on casters and provides 4 air 
exchanges 
per hour.  It's not cheap at $1000, but well worth it if it will provide 
relief for this employee and allow her to continue her employment.  Our 
lab 
is approximately 800-1000 sq. ft in size with 8 foot ceilings.  We have 1 
standard bio-hood for processing cytology fluids and 2 wall-mounted "air 
suckers" above our processors.  I am open to any suggestions.

Thanks,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas




NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a 
doctor accepting new patients.  Call (785) 354-5225.

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The information transmitted in this e-mail and in any replies and forwards 
are for the sole use of the above individual(s) or entities and may 
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From darylm <@t> propathwy.com  Wed Sep 28 06:30:46 2011
From: darylm <@t> propathwy.com (darylm@propathwy.com)
Date: Wed Sep 28 06:30:50 2011
Subject: [Histonet] Amount of work per tech
Message-ID: <20110928043046.45b0738187c28ac9fb0c6e68fbea1d97.7679898941.wbe@email16.secureserver.net>


   Hello,

   I  was wondering if there are any    of  work  a  histology tech can do per year   around  9000  cases per  year      manual  special  staining  and automated (   just one bench histology tech/supervisor.  
   Thanks,

   Daryl A. Mikita, HT(ASCP)cm

   <
From rsrichmond <@t> gmail.com  Wed Sep 28 06:32:13 2011
From: rsrichmond <@t> gmail.com (Bob Richmond)
Date: Wed Sep 28 06:32:18 2011
Subject: [Histonet] Re: Feulgen stain
Message-ID: 

Elizabeth Chlipala wrote:

>>Does anyone out there know if you can purchase this reagent, from my searches it looks like a modified Schiff reagent.  When someone is requesting this stain as the Feulgen and Rossenbeck is it just the Feulgen stain?<<

The Feulgen stain for DNA uses hydrochloric acid to cleave vicinal
diol bonds and oxidize them to aldehyde groups that are then
demonstrated with Schiff reagent. The specificity of the stain rests
with the hydrochloric acid step - concentration, time, and
temperature.

I doubt a special Schiff reagent is needed. Brewing your own Schiff
reagent tends to be a prescription for misery. I worked in a research
histochemistry lab about 40 years ago where we brewed our own (Menzies
method) but I couldn't see it really made a lot of difference from the
commercial (then Harleco) reagent.

Bob Richmond
Samurai Pathologist
Knoxville TN

From Jane.Moose <@t> NewberryHospital.net  Wed Sep 28 06:44:22 2011
From: Jane.Moose <@t> NewberryHospital.net (Jane C. Moose)
Date: Wed Sep 28 06:44:27 2011
Subject: [Histonet] KP Markers
In-Reply-To: 
Message-ID: <8BB5FC36DDA373488E60177757BBD698C49844@ncmhexchbe01.NewberryHospital.net>

We have purchased from Master Tech  in the past if you want to check.
Thanks Jane

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M.
Gorham
Sent: Tuesday, September 27, 2011 5:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] KP Markers

I received a call from Mercedes Medical saying they can not get the KP
Markers so many of us like.  Does any one have another source for these
wonderful pens?  Thanks and have a wonderful evening, Kathy Gorham H.T.


GRH National Recognition
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Leader in Innovative Excellence 2009 awarded by the OAHHS
Financial Excellence Award 2010 awarded by the national Rural Health
Research & Policy Analysis Center
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Satisfaction 2010 awarded by Amerinet
Health Devices Achievement Award 2011 awarded by ECRI Institute

GRH Mission
We will ensure access to high-quality, cost-effective health services in
a safe, customer-friendly environment for all those in need of our
services.


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From ccrowder <@t> vetmed.lsu.edu  Wed Sep 28 07:52:08 2011
From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder)
Date: Wed Sep 28 07:53:49 2011
Subject: [Histonet] Toluidine Blue
Message-ID: 

Henk -  Mast cells, on tissue and/or cytospins, are pH dependent - 2.0-2.5. 
Tissues  are usually already fixed in formalin; cytospins can be fixed in 
95% alcohol or formalin.  I am sending our directions separately.  The stain 
should less than 5 minutes totally.

Cheryl        
From rjbuesa <@t> yahoo.com  Wed Sep 28 09:24:46 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Sep 28 09:24:49 2011
Subject: [Histonet] Amount of work per tech
In-Reply-To: <20110928043046.45b0738187c28ac9fb0c6e68fbea1d97.7679898941.wbe@email16.secureserver.net>
Message-ID: <1317219886.18240.YahooMailClassic@web65714.mail.ac4.yahoo.com>

The "standard" benchmark for one HT that "does everything" as you describe is around 9,000 blocks.
Under separate cover I am sending you data on the subject.
By the way, the standard for 9,000 cases would be around 27,000 blocks only. You are preparing more blocks/case than the standard.
Ren? J.

--- On Wed, 9/28/11, darylm@propathwy.com  wrote:


From: darylm@propathwy.com 
Subject: [Histonet] Amount of work per tech
To: Histonet@lists.utsouthwestern.edu
Date: Wednesday, September 28, 2011, 7:30 AM



???Hello,

???I? was wondering if there are any = standards out there for the amount
???of? work? a? histology tech can do per year= ?? We are currently doing
???around? 9000? cases per? year???=? ;(approximately 45,000 blocks) with
???manual? special? staining? and automated (= old Dako Autostainer) with
???just one bench histology tech/supervisor.? 
???Thanks,

???Daryl A. Mikita, HT(ASCP)cm

???<= br>
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From billodonnell <@t> catholichealth.net  Wed Sep 28 09:48:48 2011
From: billodonnell <@t> catholichealth.net (O'Donnell, Bill)
Date: Wed Sep 28 09:49:18 2011
Subject: [Histonet] OK, I give up
In-Reply-To: <1317219886.18240.YahooMailClassic@web65714.mail.ac4.yahoo.com>
References: <20110928043046.45b0738187c28ac9fb0c6e68fbea1d97.7679898941.wbe@email16.secureserver.net>
	<1317219886.18240.YahooMailClassic@web65714.mail.ac4.yahoo.com>
Message-ID: <4940DF6D1C5FDF48931B6966AAEF93951F2CB3@chimsx08.CHI.catholichealth.net>


 Why does methanol come in glass containers? Is anyone getting it from a
source that does not use glass? MSDS does not say it is reactive with
plastic. I'm sure someone out there can help me

William (Bill) O'Donnell, HT (ASCP) QIHC 
Senior Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.
Cultivate it in PRAYER!


From duffettc <@t> sjhmc.org  Wed Sep 28 10:00:29 2011
From: duffettc <@t> sjhmc.org (Duffett, Christopher)
Date: Wed Sep 28 10:01:11 2011
Subject: [Histonet] Microwave ventilation
Message-ID: <3AC59012F7CF5F499F6C5718F0C16DB404B0B2D9@itr-exch01.sjhmc.sjhealthsys.org>

Hi All,

I am looking for anyone that is using a vented microwave for special
stains. I need a company to come and inspect the microwave to ensure the
ventilation  system is working correctly. Any help would be great. 

Chris Duffett 
Pathology Manager
St. Joseph's Regional Medical Center
703 Main Street
Paterson, NJ 07503 
Office 973-754-3541
Fax 973-754-3649
duffettc@sjhmc.org



CONFIDENTIAL COMMUNICATION:
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From laurie.colbert <@t> huntingtonhospital.com  Wed Sep 28 10:02:32 2011
From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert)
Date: Wed Sep 28 10:02:35 2011
Subject: [Histonet] OK, I give up
In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93951F2CB3@chimsx08.CHI.catholichealth.net>
References: <20110928043046.45b0738187c28ac9fb0c6e68fbea1d97.7679898941.wbe@email16.secureserver.net><1317219886.18240.YahooMailClassic@web65714.mail.ac4.yahoo.com>
	<4940DF6D1C5FDF48931B6966AAEF93951F2CB3@chimsx08.CHI.catholichealth.net>
Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2B165@EXCHANGE3.huntingtonhospital.com>

I've ordered it from Cardinal and Fisher and it comes in plastic
bottles.
Laurie

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
O'Donnell, Bill
Sent: Wednesday, September 28, 2011 7:49 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] OK, I give up


 Why does methanol come in glass containers? Is anyone getting it from a
source that does not use glass? MSDS does not say it is reactive with
plastic. I'm sure someone out there can help me

William (Bill) O'Donnell, HT (ASCP) QIHC 
Senior Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.
Cultivate it in PRAYER!


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From rjbuesa <@t> yahoo.com  Wed Sep 28 10:05:32 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Sep 28 10:05:38 2011
Subject: [Histonet] Clearium mounting medium: please note!
In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93951F2CB3@chimsx08.CHI.catholichealth.net>
Message-ID: <1317222332.603.YahooMailClassic@web65702.mail.ac4.yahoo.com>

Today there was a posting regarding Clearium (from Leica Micro-systems) where it was said that it allows going directly from iso-propyl alcohol to coverslippin, avoiding the use of xylene.
?
I did not know that so I found the MSDS for Clearium that reads:
?
"Main hazards: highly flammable. Irritating to respiratory system and skin. May cause sensitization by skin contact. 
Harmful: danger of serious damage to health by prolonged exposure through inhalation. May cause harm to the unborn child. Possible risk of impaired fertility. Vapours may cause drowsiness and dizziness".
?
It seems that in this specific case it can be applied the saying of "Better deal with the devil you know (xylene) than with the one you don't (Clearium)"
?
Clearium is composed of TOLUENE (10-30%) + METHYL METRACRYLATE (50-70%) + DIBUTYL PHTHALATE (1-10%).
?
My recommendation to anybody trying to use a new product only known through a "sales pitch" to check the MSDS.
?
It seeems that Clearium is not that better than xylene after all even when you can mount directly from 2-propanol eliminating a "dangerous" spet with xylene!
Ren? J.

From gu.lang <@t> gmx.at  Wed Sep 28 10:05:58 2011
From: gu.lang <@t> gmx.at (Gudrun Lang)
Date: Wed Sep 28 10:06:08 2011
Subject: AW: [Histonet] Feulgan and Rossenbeck
In-Reply-To: <14E2C6176416974295479C64A11CB9AE1DECBA0A88@SBS2K8.premierlab.local>
References: <14E2C6176416974295479C64A11CB9AE1DECBA0A88@SBS2K8.premierlab.local>
Message-ID: <84244B1B51CD46D1A8C94BC67C602C65@dielangs.at>

Feulgen and Rossenbeck developed this stain. Feulgen was the doctor,
Rosenbeck the labworker. Somehow the name Rosenbeck lapsed into oblivision.
Gudrun

-----Urspr?ngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Elizabeth
Chlipala
Gesendet: Dienstag, 27. September 2011 22:56
An: Histonet Listserv (E-mail)
Betreff: [Histonet] Feulgan and Rossenbeck

Hello everyone

Does anyone out there know if you can purchase this reagent, from my
searches it looks like a modified Schiff reagent.  When someone is
requesting this stain as the Feulgan and Rossenbeck is it just the Feulgan
stain?  Sorry this question seems really basic but to be honest I'm a bit
stumped over it.  Thanks in advance.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

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From rjbuesa <@t> yahoo.com  Wed Sep 28 10:07:12 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Sep 28 10:07:20 2011
Subject: [Histonet] Microwave ventilation
In-Reply-To: <3AC59012F7CF5F499F6C5718F0C16DB404B0B2D9@itr-exch01.sjhmc.sjhealthsys.org>
Message-ID: <1317222432.34164.YahooMailClassic@web65707.mail.ac4.yahoo.com>

The best configuration is connecting the oven directly to the fumes hood. I can send you a photo of my installation if you want.
Ren? J.

--- On Wed, 9/28/11, Duffett, Christopher  wrote:


From: Duffett, Christopher 
Subject: [Histonet] Microwave ventilation
To: Histonet@lists.utsouthwestern.edu
Date: Wednesday, September 28, 2011, 11:00 AM


Hi All,

I am looking for anyone that is using a vented microwave for special
stains. I need a company to come and inspect the microwave to ensure the
ventilation? system is working correctly. Any help would be great. 

Chris Duffett 
Pathology Manager
St. Joseph's Regional Medical Center
703 Main Street
Paterson, NJ 07503 
Office 973-754-3541
Fax 973-754-3649
duffettc@sjhmc.org



CONFIDENTIAL COMMUNICATION:
THIS TRANSMISSION IS INTENDED ONLY FOR THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND CONTAINS INFORMATION THAT IS CONFIDENTIAL. IF YOU HAVE RECEIVED THIS COMMUNICATION IN ERROR, PLEASE DELETE THE EMAIL AND CONTACT THE SENDER IMMEDIATELY. THIS INFORMATION MAY HAVE BEEN DISCLOSED TO YOU FROM CONFIDENTIAL RECORDS AND MAY BE PROTECTED BY FEDERAL AND STATE LAW. THIS INFORMATION MAY INCLUDE CONFIDENTIAL MENTAL HEALTH, SUBSTANCE ABUSE, ALCOHOL ABUSE AND/OR HIV-RELATED INFORMATION. FEDERAL AND STATE LAW PROHIBITS YOU FROM MAKING ANY FURTHER DISCLOSURE OF THIS INFORMATION WITHOUT THE SPECIFIC WRITTEN CONSENT OF THE PERSON TO WHOM IT PERTAINS, OR AS OTHERWISE PERMITTED BY LAW. ANY UNAUTHORIZED FURTHER DISCLOSURE IN VIOLATION OF THE LAW MAY RESULT IN A FINE OR JAIL SENTENCE OR BOTH. A GENERAL AUTHORIZATION FOR THE RELEASE OF THIS INFORMATION MAY NOT BE SUFFICIENT AUTHORIZATION FOR FURTHER DISCLOSURE.
_______________________________________________
Histonet mailing list
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From AHutton <@t> dh.org  Wed Sep 28 10:05:49 2011
From: AHutton <@t> dh.org (Hutton, Allison)
Date: Wed Sep 28 10:08:08 2011
Subject: [Histonet] peloris validation
Message-ID: <38A56C4F4630D348A50B3720409270870E0FE42D@dhmail.dhorg.org>

We are considering purchasing a Peloris II. We are unsure of the validation process of a processor, having not had to replace one in 15+ years. We currently have 2 VIPs. For those of you using the Peloris, How did you validate? how many runs, and what  type of tissue did you use? 
Thank you for your in put.
Allison

From tpodawiltz <@t> lrgh.org  Wed Sep 28 10:11:59 2011
From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas)
Date: Wed Sep 28 10:12:10 2011
Subject: [Histonet] RE: Microwave ventilation
In-Reply-To: <3AC59012F7CF5F499F6C5718F0C16DB404B0B2D9@itr-exch01.sjhmc.sjhealthsys.org>
References: <3AC59012F7CF5F499F6C5718F0C16DB404B0B2D9@itr-exch01.sjhmc.sjhealthsys.org>
Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DF21AB96@LRGHEXVS1.practice.lrgh.org>

Mine is ducted directly into our ventilation system 


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Duffett, Christopher
Sent: Wednesday, September 28, 2011 11:00 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microwave ventilation

Hi All,

I am looking for anyone that is using a vented microwave for special
stains. I need a company to come and inspect the microwave to ensure the
ventilation  system is working correctly. Any help would be great. 

Chris Duffett 
Pathology Manager
St. Joseph's Regional Medical Center
703 Main Street
Paterson, NJ 07503 
Office 973-754-3541
Fax 973-754-3649
duffettc@sjhmc.org



CONFIDENTIAL COMMUNICATION:
THIS TRANSMISSION IS INTENDED ONLY FOR THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND CONTAINS INFORMATION THAT IS CONFIDENTIAL. IF YOU HAVE RECEIVED THIS COMMUNICATION IN ERROR, PLEASE DELETE THE EMAIL AND CONTACT THE SENDER IMMEDIATELY. THIS INFORMATION MAY HAVE BEEN DISCLOSED TO YOU FROM CONFIDENTIAL RECORDS AND MAY BE PROTECTED BY FEDERAL AND STATE LAW. THIS INFORMATION MAY INCLUDE CONFIDENTIAL MENTAL HEALTH, SUBSTANCE ABUSE, ALCOHOL ABUSE AND/OR HIV-RELATED INFORMATION. FEDERAL AND STATE LAW PROHIBITS YOU FROM MAKING ANY FURTHER DISCLOSURE OF THIS INFORMATION WITHOUT THE SPECIFIC WRITTEN CONSENT OF THE PERSON TO WHOM IT PERTAINS, OR AS OTHERWISE PERMITTED BY LAW. ANY UNAUTHORIZED FURTHER DISCLOSURE IN VIOLATION OF THE LAW MAY RESULT IN A FINE OR JAIL SENTENCE OR BOTH. A GENERAL AUTHORIZATION FOR THE RELEASE OF THIS INFORMATION MAY NOT BE SUFFICIENT AUTHORIZATION FOR FURTHER DISCLOSURE.
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From algranth <@t> email.arizona.edu  Wed Sep 28 10:10:46 2011
From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth))
Date: Wed Sep 28 10:13:57 2011
Subject: AW: [Histonet] Feulgan and Rossenbeck
In-Reply-To: <84244B1B51CD46D1A8C94BC67C602C65@dielangs.at>
References: <14E2C6176416974295479C64A11CB9AE1DECBA0A88@SBS2K8.premierlab.local>
	<84244B1B51CD46D1A8C94BC67C602C65@dielangs.at>
Message-ID: <49A293B2-692F-4304-972B-F2E5FB369A88@email.arizona.edu>







On Sep 28, 2011, at 8:05 AM, Gudrun Lang wrote:

> Feulgen and Rossenbeck developed this stain. Feulgen was the doctor,
> Rosenbeck the labworker. Somehow the name Rosenbeck lapsed into oblivision.


This tells us something doesn't it?
From cbobrowi <@t> mcw.edu  Wed Sep 28 10:19:13 2011
From: cbobrowi <@t> mcw.edu (Bobrowitz, Carol)
Date: Wed Sep 28 10:20:16 2011
Subject: [Histonet] Xylene exposure???
Message-ID: <8F78639AC56F4143B267FE5F5A1B92C803656FF1@guyton.phys.mcw.edu>

Hello,

 

I've been monitoring the discussion about Xylene.

 

No one has mentioned Latex.

 

I have the exact symptoms but from Latex.

 

Our lab is Latex free (???) but there are many items impossible to
remove.

Yes, we use powder free nitrile gloves.

Examples:  floor tiles, paint on the walls, shoes, vacuum seals on the
processor, the vortex.

 

Bananas should not be eaten with a Latex allergy. (Go figure)

 

About 15 years ago a supervisor developed a Latex sensitivity and needed
to resign

her position in a major Milwaukee histology lab.

This was sudden. 

Her home needed to be stripped of latex paint, carpeting and padding
removed.  

Your home is loaded with latex products.

 

Back to Xylene.

 

You will NEVER be removed from Xylene fumes in a histology lab.

Your intensity over time will only get worse.

 

Yes, I understand there are substitutes and/or mineral oil.

Yes, you can wear masks, work further away from the usage, work as a
team and

depend on fellow techs to perform those duties etc, etc, etc.

 

Even if you work with the architects to design a lab with excellent
ventilation and air flow, 

you will still be sucking up those fumes.  You just can't smell them any
more.

  

Did you know not all hoods remove Xylene???

 

Over the years I have seen many changes in histology.

This is safer than the old one but to find out the replacement is worse
then the first.

 

The pathology lab I transferred from was next to the morgue.  A water
main broke and flooded

the morgue and from under the door came many years of mold and whatever
into our histo lab.

The blood too came in.  The Docs and PA's were doing autopsies with hip
waders on.

After cleaning up the mess I was sick for months.

 

The ceiling tiles around the vents were caked with black cigarette tar
from years ago when techs smoked, yes

also during coverslipping from Xylene.

 

The cock roaches were huge and you had to be careful using the bathroom
for fear they'd go up

your leg. We had to be careful not to step on a female roach carrying
eggs.  

Those eggs went home on your shoes.

 

We had absolutely no ventilation for Xylene or Formaldehyde.

 

Disel fumes from the loading dock were sucked into the lab. (needed to
be put on oxygen once)

 

Keeping extra shoes at work wasn't a good idea.  The mice. 

 

Yes, I did try to change things but sometimes the powers above are
difficult to deal with.

Finally someone listened.  They tore the building down.

 

I credit the younger generations of histology personnel for their
continual due diligence 

making histology labs a safer environment.  Thank you and keep up the
excellent work.

 

Supervisors or managers can only do so much until their hands are tied.

  

Choosing to work in a Histology Lab is your choice.

 

Day 1 you know about the hazards and challenges.

 

Every Histology book written mentions the down sides.

 

If you are paranoid and not happy with the conditions it's your
individual choice to work in Histology.

 

It is your personal responsibility to eliminate hazards to your health.
(some you can, some you can't)

 

In the past forty years Histology has changed with leaps and bounds.

 

So much good has come to Histology.

 

In clinical pathology labs and research labs Histology has an open
future.

 

Just think maybe, no Xylene, Formalin or DAB.

 

I really want to see the future from my rocking chair.

 

Yes, Glen, we miss the old lab.

 

Respectfully,

 

Carol Ann Bobrowitz HT/HTL/ASCP

Medical College of Wisconsin

cbobrowi@mcw.edu

 

 

 

 

 

From rjbuesa <@t> yahoo.com  Wed Sep 28 10:23:21 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Sep 28 10:23:28 2011
Subject: [Histonet] peloris validation
In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE42D@dhmail.dhorg.org>
Message-ID: <1317223401.15927.YahooMailClassic@web65706.mail.ac4.yahoo.com>

Select a series of different tissues (as diverse as possible) ?and run half with your VIP and the other half with the Peloris. Do not identify which processor was used for each block/section and give the sections to as many pathologists in unidentified pairs as? a "blind test" They will not know how each block was processed.
?
The referees will have only two choices in their selection:
1- one section was better than the other within the pair (V or P), or
2 both were the same = of equal quality?(S).
Analyze the results with the chi-square test where "S" includes V and P for a theoretical frequency of
(S+P) = (S+V) = 1.00 and for each is the theoreticla frequency is 0.5?
Compare the theoretical distribution with the results obtained from the referees.
?
By the way, the Peloris the existing "official" validations were done in Australia by the manufacturers of the original instrument (VisionBioSystems) and were never externally corroborated.
Ren? J.
?
?

--- On Wed, 9/28/11, Hutton, Allison  wrote:


From: Hutton, Allison 
Subject: [Histonet] peloris validation
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, September 28, 2011, 11:05 AM


We are considering purchasing a Peloris II. We are unsure of the validation process of a processor, having not had to replace one in 15+ years. We currently have 2 VIPs. For those of you using the Peloris, How did you validate? how many runs, and what? type of tissue did you use? 
Thank you for your in put.
Allison

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From baderbo <@t> gmail.com  Wed Sep 28 10:54:49 2011
From: baderbo <@t> gmail.com (Bader Siddiki)
Date: Wed Sep 28 10:54:53 2011
Subject: [Histonet] Xylene
Message-ID: 

Hello Histonetters
I have been reading about xylene, how dangerous it is.
Have you thought about using mounting medium made from Limonene.
Here is some info:

*AR-6504 Organo (Limonene) mount?:** *This mounting medium is made with
limonene a natural product from orange peels. It is good for preserving
tissues and cell smears that can be dehydrated with organic solvents in
Immunohistochemistry (IHC) e.g. DAB and DAB with nickel or cobalt. This
Organo mounting medium is also suitable with alkaline phosphatase
chromogens, an organic solvent resistant Super Fast Red (IBSC, cat AR-8211).
It is an excellent choice for mounting H and E stained slides. Coverslip is
required. *DIRECT SUBSTITUTE OF Biomeda?s Clarion*
This mounting medium is made by ImmunoBioScience corp.
www.ImmunoBioScience.Com




Have a nice day/weekend
Mit freundlichen Gr??en / With Kind Regards /
avec l'aimable ce qui concerne
Met vriendelijke groeten
??????
Bader
Executive director,
Research and development
ImmunoBioScience Corp. (IBSC)
Phone: + 1 425 367 4601
Fax: + 1 425 367 4817
cell (mobile) phone: + 1 425 314 0199
e-mail address: BaderBo@Gmail.com
Web site: www.ImmunoBioScience.Com
Marketing: phone: + 1 650 343 IBSC (4272)
                E-mail: AnitaIBSC@Aol.com
From SDattili <@t> stormontvail.org  Wed Sep 28 11:16:09 2011
From: SDattili <@t> stormontvail.org (D'Attilio, Shelley)
Date: Wed Sep 28 11:16:27 2011
Subject: [Histonet] Thanks!
In-Reply-To: <456da2a9000f380e@stormontvail.org>
Message-ID: 

Hi all,
I wanted to thank all of you who responded to my question about my xylene-sensitive employee and purchase of an air filter system.  I got tons of terrific information and enjoyed the discussion about xylene substitutes.  Those who responded gave me a lot to think about and raised some questions for me to have answered.

It's wonderful to have access to all of you experts on the listserv!  Thank you for your generosity.

Regards,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas 



NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.  Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.  If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.  Thank you for your cooperation.

******************************************************************************************************************

From Kelly.Buechel <@t> thedacare.org  Wed Sep 28 11:50:41 2011
From: Kelly.Buechel <@t> thedacare.org (Kelly Buechel)
Date: Wed Sep 28 11:50:47 2011
Subject: [Histonet] FORMALIN RECYCLER BY CREATIVE WASTES SOLUTIONS, ISSUES?
Message-ID: <58549F7DF7F9444DB732F84F55B1051FECFF387B@EXMB2.ad.local>

HI, my lab purchased  the Creative Waste Solutions Bench Top Formalin Recycler a year ago in October and we're considering discontinuing use because of some issues. I am posting this question hoping someone else has had similar issues and maybe has resolved them or does something we're not.

When we purchased the recycler we were told we could use the recycled formalin  for fixing any tissue (even breast and colon), and in our VIPS (processors).  We were also told by the company that we could recycle formalin from just about anywhere except from any fatty tissue or anything too bloody.

After a few months of use one of our pathologists complained that  the breasts weren't being properly fixed by the recycled formalin.  So we stopped using recycled formalin on breast tissue and on the processor that ran the extended run.

A few months after we cut back, the recycler was dispensing colored recycled formalin.  The company told me that this would happen, and that we must purchase two recyclers to filter one gallon of formalin from now on.   One recycler to put the dirty/discolored formalin thru, then a second recycler to clean the one gallon all ready recycled formalin so that it comes out clear.  We weren't recycling anything bloody, but the dyes from tissues would turn the formalin many colors.  Since we weren't sure if we were keeping the recycler and don't have a lot of space, why would we buy a second one?  So..we had to really limit what we could recycle.

Over the summer there have been many complaints about the way the tissue looks on the slides and questions if everything is being properly fixed.  We test the pH of every bottle of recycled formalin before use.  We do maintenance on the BTFRS monthly when it gets backed up and then assay the formalin until the correct percentage is found.  But still people were concerned that if we shouldn't use the recycled formalin on breast tissue, then why should we use it on any other tissue?  And that's where we are at.  We want to find a way to not dump formalin down the drain, but also fix our specimens the best way possible.  A formalin neutralizer seems like the way to go, but recycling sure did have its perks.

Can anyone relate or have any thoughts?
From Reuel.Cornelia <@t> tsrh.org  Wed Sep 28 12:06:59 2011
From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia)
Date: Wed Sep 28 12:07:26 2011
Subject: [Histonet] Thanks!
In-Reply-To: 
References: <456da2a9000f380e@stormontvail.org>
	
Message-ID: <4E830DE3.077E.00C5.1@tsrh.org>

This was a good discussion and a some little rough comment  but we learn from it. I have read all the feedback on xylene and its substitute and it  gives us a lesson to become more better histotechs. Just this morning one of our administrator passed by near our processor while my co worker has just opened the processor retort for cleaning and smelled xylene in the air. He stopped and asked us why he was not using a mask while doing that. I replied that was only a minimal amount of xylene in the retort that was left after the cleaning cycle and he said well I just want to make sure of your safety. When we are so used of the smell sometimes we do not think of our Safety and this discussion taught us again to think first of our own Safety whether you are sensitive or not to xylene or any chemical.
To shelly, I wish your employee the best and all success in the field of histology.
 
 
Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768

>>> "D'Attilio, Shelley"  9/28/2011 11:16 AM >>>
Hi all,
I wanted to thank all of you who responded to my question about my xylene-sensitive employee and purchase of an air filter system.  I got tons of terrific information and enjoyed the discussion about xylene substitutes.  Those who responded gave me a lot to think about and raised some questions for me to have answered.

It's wonderful to have access to all of you experts on the listserv!  Thank you for your generosity.

Regards,

Shelley D'Attilio MT(ASCP)
Manager, Chemistry, Cytology and Histology
Dept. of Pathology and Laboratory Medicine
Stormont-Vail HealthCare
Topeka, Kansas 



NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a doctor accepting new patients.  Call (785) 354-5225.

******************************************************************************************************************

The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information.  Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited.  If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address.  Thank you for your cooperation.

******************************************************************************************************************

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

From nicole <@t> dlcjax.com  Wed Sep 28 13:10:37 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Wed Sep 28 13:16:47 2011
Subject: [Histonet] Xylene
In-Reply-To: 
References: 
Message-ID: <3025.208.62.167.196.1317233437.squirrel@webmail.realpages.com>

Might not be toxic but the fumes of citrus give me headaches and the smell
is terrible.. Im a slide bright girl, no odor, and non flammable

 Hello Histonetters
> I have been reading about xylene, how dangerous it is.
> Have you thought about using mounting medium made from Limonene.
> Here is some info:
>
> *AR-6504 Organo (Limonene) mount???:** *This mounting medium is made with
> limonene a natural product from orange peels. It is good for preserving
> tissues and cell smears that can be dehydrated with organic solvents in
> Immunohistochemistry (IHC) e.g. DAB and DAB with nickel or cobalt. This
> Organo mounting medium is also suitable with alkaline phosphatase
> chromogens, an organic solvent resistant Super Fast Red (IBSC, cat
> AR-8211).
> It is an excellent choice for mounting H and E stained slides. Coverslip
> is
> required. *DIRECT SUBSTITUTE OF Biomeda???s Clarion*
> This mounting medium is made by ImmunoBioScience corp.
> www.ImmunoBioScience.Com
>
>
>
>
> Have a nice day/weekend
> Mit freundlichen Gr????en / With Kind Regards /
> avec l'aimable ce qui concerne
> Met vriendelijke groeten
> ??????????????????
> Bader
> Executive director,
> Research and development
> ImmunoBioScience Corp. (IBSC)
> Phone: + 1 425 367 4601
> Fax: + 1 425 367 4817
> cell (mobile) phone: + 1 425 314 0199
> e-mail address: BaderBo@Gmail.com
> Web site: www.ImmunoBioScience.Com
> Marketing: phone: + 1 650 343 IBSC (4272)
>                 E-mail: AnitaIBSC@Aol.com
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



From nicole <@t> dlcjax.com  Wed Sep 28 13:23:42 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Wed Sep 28 13:29:49 2011
Subject: [Histonet] Xylene alternative
Message-ID: <3181.208.62.167.196.1317234222.squirrel@webmail.realpages.com>

Summary & Explanation:
SLIDE BRITE is a revolutionary dewaxing and clearing reagent for histologic
techniques. It is a safe and effective alternative to xylene. It contains
no carcinogens,
no toxins, is odorless, and is classified as non-flammable and
non-hazardous. Its flash
point is above 140?F (almost double of that of xylene).
A qualified laboratory that is certified by the State of California Health
Services has
performed toxicity screening. SLIDE BRITE was designated non-hazardous on the
basis of aquatic toxicity; thus eliminating hazardous waste and exposure
to women of
child-bearing age to xylene and cancer-causing reagents.

It has non hazad shipping.
No orange smell.
does not have to be disposed of with hazardious chemicals.
non-toxic.
does not need to be stored in fire caninet.

The only draw back I can find is it becomes saturated alot easier than
xylene.

Nicole Tatum, HT ASCP


From trathborne <@t> somerset-healthcare.com  Wed Sep 28 13:56:10 2011
From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni)
Date: Wed Sep 28 13:56:16 2011
Subject: [Histonet] Xylene alternative
In-Reply-To: <3181.208.62.167.196.1317234222.squirrel@webmail.realpages.com>
References: <3181.208.62.167.196.1317234222.squirrel@webmail.realpages.com>
Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F58BF8@SMCMAIL01.somerset-healthcare.com>

Do you use this product for processing and staining? Do you need to use a particular type of mounting medium? How is it disposed of?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum
Sent: Wednesday, September 28, 2011 2:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene alternative

Summary & Explanation:
SLIDE BRITE is a revolutionary dewaxing and clearing reagent for histologic techniques. It is a safe and effective alternative to xylene. It contains no carcinogens, no toxins, is odorless, and is classified as non-flammable and non-hazardous. Its flash point is above 140?F (almost double of that of xylene).
A qualified laboratory that is certified by the State of California Health Services has performed toxicity screening. SLIDE BRITE was designated non-hazardous on the basis of aquatic toxicity; thus eliminating hazardous waste and exposure to women of child-bearing age to xylene and cancer-causing reagents.

It has non hazad shipping.
No orange smell.
does not have to be disposed of with hazardious chemicals.
non-toxic.
does not need to be stored in fire caninet.

The only draw back I can find is it becomes saturated alot easier than xylene.

Nicole Tatum, HT ASCP


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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From christiegowan <@t> msn.com  Wed Sep 28 14:11:44 2011
From: christiegowan <@t> msn.com (CHRISTIE GOWAN)
Date: Wed Sep 28 14:11:48 2011
Subject: [Histonet] job opening at Vanderbilt University Medical Center
In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F58BF8@SMCMAIL01.somerset-healthcare.com>
References: <3181.208.62.167.196.1317234222.squirrel@webmail.realpages.com>,
	<3AD061FE740D464FAC7BF6B5CFB7570711F58BF8@SMCMAIL01.somerset-healthcare.com>
Message-ID: 


Dear Netters,
I am posting this for a friend who is not on Histonet. The following is a link to a job opening at Vanderbilt University Medical Center. Please respond to the link if interested.
 
 
https://www.recruitingcenter.net/Clients/vanderbiltjobs/PublicJobs/controller.cfm?jbaction=JobProfile&job_id=30391

 


(ANOTHER LINK: http://www.jobtarget.com/link.cfm?c=SFrHQBOw83vA )
 
Thanks,
Christie Gowan 		 	   		  
From nicole <@t> dlcjax.com  Wed Sep 28 14:08:23 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Wed Sep 28 14:14:30 2011
Subject: [Histonet] Xylene alternative
In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC01D09C@JERRY.Gia.com>
References: <3181.208.62.167.196.1317234222.squirrel@webmail.realpages.com>
	<5A33C952BB67F4468AF1F36D739212BC01D09C@JERRY.Gia.com>
Message-ID: <3283.208.62.167.196.1317236903.squirrel@webmail.realpages.com>

Amber,

I have a dermpath/ mohs lab and I use slide bright for everything. I have
used the product for 9yrs now, at least.  Slide bright is in my processor,
H%E stain line and my deparrafin stain line. Definitly call and get
sample. I love it. I get mine from Mercedes medical, belair(advantik),
PSS, statlab, ect.  I think 4gallons cost more than xylene, but save on
shipping, disposal, and my overall health, so for me its a no brainer. The
only strong odor I have is when I coverslip.

PS does not counteract with acid or bluing. Works with all chemicals
Good luck,
Nicole Tatum HT, aSCP




Who sells Slide Brite?  Is that a cardinal product?  Can you use it with
> regular alcohol, bluing and Acid alcohol on the H&E stainer? That sounds
> like a great product to try in subbing for the xylene.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole
> Tatum
> Sent: Wednesday, September 28, 2011 1:24 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Xylene alternative
>
> Summary & Explanation:
> SLIDE BRITE is a revolutionary dewaxing and clearing reagent for
> histologic
> techniques. It is a safe and effective alternative to xylene. It contains
> no carcinogens,
> no toxins, is odorless, and is classified as non-flammable and
> non-hazardous. Its flash
> point is above 140?F (almost double of that of xylene).
> A qualified laboratory that is certified by the State of California Health
> Services has
> performed toxicity screening. SLIDE BRITE was designated non-hazardous on
> the
> basis of aquatic toxicity; thus eliminating hazardous waste and exposure
> to women of
> child-bearing age to xylene and cancer-causing reagents.
>
> It has non hazad shipping.
> No orange smell.
> does not have to be disposed of with hazardious chemicals.
> non-toxic.
> does not need to be stored in fire caninet.
>
> The only draw back I can find is it becomes saturated alot easier than
> xylene.
>
> Nicole Tatum, HT ASCP
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



From dchihc <@t> yahoo.com  Wed Sep 28 14:14:51 2011
From: dchihc <@t> yahoo.com (Phyllis Thaxton)
Date: Wed Sep 28 14:15:54 2011
Subject: [Histonet] P 16
Message-ID: <1317237291.78880.YahooMailNeo@web43513.mail.sp1.yahoo.com>

Has anyone ever used P16 with AP Red detection on the Ventana Benchmark?


Phyllis Jordan HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
From nicole <@t> dlcjax.com  Wed Sep 28 14:16:22 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Wed Sep 28 14:22:36 2011
Subject: [Histonet] Xylene alternative
In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F58BF8@SMCMAIL01.somerset-healthcar
	e.com>
References: <3181.208.62.167.196.1317234222.squirrel@webmail.realpages.com>
	<3AD061FE740D464FAC7BF6B5CFB7570711F58BF8@SMCMAIL01.somerset-healthcare.com>
Message-ID: <3301.208.62.167.196.1317237382.squirrel@webmail.realpages.com>

Toni,

Slide bright is everywhere xylene would have been. Proceesor, stainline, etc.

I use either TBS shur/mount( xylene based) or S mounting media (toulene
based). I think the xylene based media works better.

As per the directions it can be flushed down the drain with copious
amounts of water. But always check with your local enviromental agency.

Nicole Tatum, HT ASCP




 Do you use this product for processing and staining? Do you need to use a
> particular type of mounting medium? How is it disposed of?
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole
> Tatum
> Sent: Wednesday, September 28, 2011 2:24 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Xylene alternative
>
> Summary & Explanation:
> SLIDE BRITE is a revolutionary dewaxing and clearing reagent for
> histologic techniques. It is a safe and effective alternative to xylene.
> It contains no carcinogens, no toxins, is odorless, and is classified as
> non-flammable and non-hazardous. Its flash point is above 140??F (almost
> double of that of xylene).
> A qualified laboratory that is certified by the State of California Health
> Services has performed toxicity screening. SLIDE BRITE was designated
> non-hazardous on the basis of aquatic toxicity; thus eliminating hazardous
> waste and exposure to women of child-bearing age to xylene and
> cancer-causing reagents.
>
> It has non hazad shipping.
> No orange smell.
> does not have to be disposed of with hazardious chemicals.
> non-toxic.
> does not need to be stored in fire caninet.
>
> The only draw back I can find is it becomes saturated alot easier than
> xylene.
>
> Nicole Tatum, HT ASCP
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> CONFIDENTIALITY NOTICE
> This message and any included attachments are from Somerset Medical Center
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From mvgeducationaresources <@t> gmail.com  Wed Sep 28 14:39:05 2011
From: mvgeducationaresources <@t> gmail.com (MVG Educational)
Date: Wed Sep 28 14:39:09 2011
Subject: [Histonet] Puerto Rico Histotechnology Congress 2012
Message-ID: 

Histotechnology Congress 2012
will be Saturday January 14, 2012
Hotel El Pedregal, Aguadilla, Puerto Rico.

Spaces available for sponsors interested in participating please write to:
mvgeducationaresources@gmail.com for more information.

-- 
*Lcda. Mary V. Guerrero, B.S., HtL, MBA*
Presidente
MVG Educational Resources, Corp.
From sbaldwin <@t> mhhcc.org  Wed Sep 28 14:49:02 2011
From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org)
Date: Wed Sep 28 14:49:07 2011
Subject: [Histonet] napsin a ccontrol slides
Message-ID: 

Hey Histonetters
Where can I buy Napsin A control slides we don't have any adenocarcinoma's for internal ??

Thanks
Histology/Cytology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin@mhhcc.org
Ph 812-996-0210, 0216,  Fax 812-996-0232, 
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
From rjbuesa <@t> yahoo.com  Wed Sep 28 14:50:04 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Sep 28 14:50:09 2011
Subject: [Histonet] FORMALIN RECYCLER BY CREATIVE WASTES SOLUTIONS, ISSUES?
In-Reply-To: <58549F7DF7F9444DB732F84F55B1051FECFF387B@EXMB2.ad.local>
Message-ID: <1317239404.71009.YahooMailClassic@web65714.mail.ac4.yahoo.com>

You are in a dilemma and that is why I never recycled formalin.
Recycling formalin by filtration seems better, but you will end with the limitations you point out.
Recycling formalin by distillation involves neutralizing the product and in both cases you will be exposed to formalin more than if you just use it to fix tissues and later accumulate it for disposal by a specialized company.
The issue with formalin is to use less, to expose oneself less, ?and just use it once.
The cost of formalin plus that of disposing is worth when compared with the health risks involved in its extra handling while?recycling it, and refilling the containers to fix.
At the end, as you pointed out, the fixation is improper and this is the fundamental step in tissue processing.
Again, that is why I never recycled formalin and I think you should return to buying prefilled containers and control your exposure to formalin.
Ren? J.

--- On Wed, 9/28/11, Kelly Buechel  wrote:


From: Kelly Buechel 
Subject: [Histonet] FORMALIN RECYCLER BY CREATIVE WASTES SOLUTIONS, ISSUES?
To: "'histonet@lists.utsouthwestern.edu'" 
Date: Wednesday, September 28, 2011, 12:50 PM


HI, my lab purchased? the Creative Waste Solutions Bench Top Formalin Recycler a year ago in October and we're considering discontinuing use because of some issues. I am posting this question hoping someone else has had similar issues and maybe has resolved them or does something we're not.

When we purchased the recycler we were told we could use the recycled formalin? for fixing any tissue (even breast and colon), and in our VIPS (processors).? We were also told by the company that we could recycle formalin from just about anywhere except from any fatty tissue or anything too bloody.

After a few months of use one of our pathologists complained that? the breasts weren't being properly fixed by the recycled formalin.? So we stopped using recycled formalin on breast tissue and on the processor that ran the extended run.

A few months after we cut back, the recycler was dispensing colored recycled formalin.? The company told me that this would happen, and that we must purchase two recyclers to filter one gallon of formalin from now on.???One recycler to put the dirty/discolored formalin thru, then a second recycler to clean the one gallon all ready recycled formalin so that it comes out clear.? We weren't recycling anything bloody, but the dyes from tissues would turn the formalin many colors.? Since we weren't sure if we were keeping the recycler and don't have a lot of space, why would we buy a second one?? So..we had to really limit what we could recycle.

Over the summer there have been many complaints about the way the tissue looks on the slides and questions if everything is being properly fixed.? We test the pH of every bottle of recycled formalin before use.? We do maintenance on the BTFRS monthly when it gets backed up and then assay the formalin until the correct percentage is found.? But still people were concerned that if we shouldn't use the recycled formalin on breast tissue, then why should we use it on any other tissue?? And that's where we are at.? We want to find a way to not dump formalin down the drain, but also fix our specimens the best way possible.? A formalin neutralizer seems like the way to go, but recycling sure did have its perks.

Can anyone relate or have any thoughts?
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From cebass <@t> buffalo.edu  Wed Sep 28 21:48:07 2011
From: cebass <@t> buffalo.edu (Caroline Bass)
Date: Wed Sep 28 21:48:26 2011
Subject: [Histonet] new or used cryostat question?
Message-ID: <4E18944F-43B4-4812-8206-857FEF3D3107@buffalo.edu>

Hello All,

I'm starting my own lab and am trying to make the best use of my startup package. I section rodent brain on a sliding microtome with a dry ice stage (AO 860) but would like to move to fresh frozen sections mostly for RNA work and perhaps laser capture. I'm trying to decide whether it's better to try to use one of the many cryostats in the department (not shared, the PIs are willing assuming that it doesn't interfere with their needs) or to purchase my own. I could get a used unit or perhaps the cheapest new I could find. I'd be more interested in the cryojane system since flat brain sections are so problematic. 

I'm wondering if anyone has suggestions for my particular application in brains? Are there any used cryostats I should look out for or can you suggest a good inexpensive new model. Any brands to avoid? Any way to save money on a decent system? Is the cryojane really worth it?

Any and all comments are appreciated!

Thanks,

Caroline



From sfonner <@t> labpath.com  Thu Sep 29 05:15:09 2011
From: sfonner <@t> labpath.com (Sheila Fonner)
Date: Thu Sep 29 05:19:49 2011
Subject: [Histonet] Rocky Mt Spotted Fever
Message-ID: <000e01cc7e90$ab1963a0$014c2ae0$@com>

I know that I have requested this information once before but am in need of
it again.  Does anyone know of a company that tests for Rocky Mt Spotted
Fever?  We are having a  hard time finding someone that still carries an
antibody that tests for this.

 

Any and all info greatly appreciated.

 

Sheila

KDL Pathology

Knoxville, TN

 

 

From TGoins <@t> mt.gov  Thu Sep 29 09:19:16 2011
From: TGoins <@t> mt.gov (Goins, Tresa)
Date: Thu Sep 29 09:19:42 2011
Subject: [Histonet] Rocky Mt Spotted Fever
In-Reply-To: <000e01cc7e90$ab1963a0$014c2ae0$@com>
References: <000e01cc7e90$ab1963a0$014c2ae0$@com>
Message-ID: 

You might try contacting Rocky Mountain Lab in Hamilton Montana http://www.niaid.nih.gov/about/organization/dir/rml/Pages/default.aspx 


Tresa Goins
Veterinary Diagnostic Lab
South 19th and Lincoln
Bozeman, MT 59718
406-994-6353 - phone
406-994-6344 - fax



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner
Sent: Thursday, September 29, 2011 4:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Rocky Mt Spotted Fever

I know that I have requested this information once before but am in need of
it again.  Does anyone know of a company that tests for Rocky Mt Spotted
Fever?  We are having a  hard time finding someone that still carries an
antibody that tests for this.

 

Any and all info greatly appreciated.

 

Sheila

KDL Pathology

Knoxville, TN

 

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From Carmen.M.Garcia <@t> uv.es  Thu Sep 29 09:37:49 2011
From: Carmen.M.Garcia <@t> uv.es (Carmen Maria Garcia Pascual)
Date: Thu Sep 29 09:37:59 2011
Subject: [Histonet] IgGs
In-Reply-To: <4213787553carmaga6@uv.es>
References: <4213787553carmaga6@uv.es>
Message-ID: <5176784527carmaga6@uv.es>


Hello everybody:

I have to carry on an experiment and I want to inject to the control 
mice an human IgG, someone can recommend me any? 
Thank you very much,

Kind regards,

Carmen


From turkekul <@t> gmail.com  Thu Sep 29 10:23:35 2011
From: turkekul <@t> gmail.com (mesruh turkekul)
Date: Thu Sep 29 10:23:40 2011
Subject: [Histonet] Azure B for melanin
Message-ID: 

Dear Histology Gurus,

I read some where that one can do Azure B counter staining to stain the
melanin green to distinguish it from DAB in a IHC section that contains
brown melanin. Have anyone tried this? It seems less harmful to the
antigenicity of the tissue than bleaching with hydrogen peroxide or K
permanganate/oxalic acid treatment.


Any comments will be greatly appreciated!


Regards,
Mesruh Turkekul
mskcc.org
From sfonner <@t> labpath.com  Thu Sep 29 10:31:46 2011
From: sfonner <@t> labpath.com (Sheila Fonner)
Date: Thu Sep 29 10:36:27 2011
Subject: [Histonet] IHC H.P. Negative Control
In-Reply-To: <20139929.229728.1317146267935.JavaMail.root@mail3d.brinkster.com>
References: <12823886.222925.1317064185836.JavaMail.root@mail3d.brinkster.com>
	<20139929.229728.1317146267935.JavaMail.root@mail3d.brinkster.com>
Message-ID: <001b01cc7ebc$e667de60$b3379b20$@com>

CAP guidelines clearly state that you must run a negative control for each patient tissue.  However, if you are running a large amount of a particular antibody, you may validate your procedure to run only one positive control.  However, it is just as easy to run a positive control for each patient if you put the control on the same slide as the patient tissue.

I don't see any way around the negatives though. We run a negative control on each and every block.

Sheila


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Aperghis Kavanagh
Sent: Tuesday, September 27, 2011 1:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC H.P. Negative Control

Hi Histonetters,

We would like to start to run up to 30 H.Pylori on our Benchmark daily.  Do GI labs that run H.P. on ALL stomach biopsies run a negative control for each patient tissue for H.P.?  I am just curious, as we have a 24 hour TAT, but the Benchmark only holds 30 slides.  What's everyone doing?

Thanks!

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From sfonner <@t> labpath.com  Thu Sep 29 11:18:59 2011
From: sfonner <@t> labpath.com (Sheila Fonner)
Date: Thu Sep 29 11:23:40 2011
Subject: [Histonet] IHC H.P. Negative Control
In-Reply-To: 
References: <12823886.222925.1317064185836.JavaMail.root@mail3d.brinkster.com><20139929.229728.1317146267935.JavaMail.root@mail3d.brinkster.com>
	<001b01cc7ebc$e667de60$b3379b20$@com>
	
Message-ID: <004a01cc7ec3$7ea11470$7be33d50$@com>

Rich,

 

That is definitely true also.  I guess I just assumed that the question was
in regards to 30 separate and individual patients.  My bad if that was not
the case.  Thanks for the clarification.

 

Sheila

 

 

From: Richard Yeo [mailto:ryeo@wchosp.org] 
Sent: Thursday, September 29, 2011 12:16 PM
To: Sheila Fonner
Subject: RE: [Histonet] IHC H.P. Negative Control

 

Sheila,

 

Cap requires a negative with each block of patient tissue  being stained for
immunos, However they also state that if more than one block from the same
patient is being tested that only one of the blocks is required for the run.

 

 

Thanks Rich Y

 

  _____  

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sheila Fonner
Sent: Thu 9/29/2011 11:31 AM
To: 'Adrienne Aperghis Kavanagh'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC H.P. Negative Control

CAP guidelines clearly state that you must run a negative control for each
patient tissue.  However, if you are running a large amount of a particular
antibody, you may validate your procedure to run only one positive control.
However, it is just as easy to run a positive control for each patient if
you put the control on the same slide as the patient tissue.

I don't see any way around the negatives though. We run a negative control
on each and every block.

Sheila


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne
Aperghis Kavanagh
Sent: Tuesday, September 27, 2011 1:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC H.P. Negative Control

Hi Histonetters,

We would like to start to run up to 30 H.Pylori on our Benchmark daily.  Do
GI labs that run H.P. on ALL stomach biopsies run a negative control for
each patient tissue for H.P.?  I am just curious, as we have a 24 hour TAT,
but the Benchmark only holds 30 slides.  What's everyone doing?

Thanks!

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From tkngflght <@t> yahoo.com  Thu Sep 29 11:36:24 2011
From: tkngflght <@t> yahoo.com (Cheryl)
Date: Thu Sep 29 11:36:31 2011
Subject: [Histonet] Jobs around the country...
Message-ID: <1317314184.84819.YahooMailNeo@web39413.mail.mud.yahoo.com>

Greetings 'Netters,
?
Again (or --as always) we're looking for a few great techs!
?
If these positions don't interest you, we'll switch to 'search' mode to help you find your perfect fit...
?
Current openings in:
?
New Jersey
Colorado
Texas (a bunch including a new lab and PA or grossing?qualified)
Louisiana
New York
Maryland
Nevada
California (including a supervisory post)
Connecticut
Michigan
?
Call for more details--you'll talk with a tech so it's an easy conversation.
?
?
Cheryl Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab, one GREAT tech at a time...
800.756.3309 Office and Fax
281.852.9457 Office
From zodiac29 <@t> comcast.net  Thu Sep 29 11:46:49 2011
From: zodiac29 <@t> comcast.net (zodiac29@comcast.net)
Date: Thu Sep 29 11:46:52 2011
Subject: [Histonet] Equipment maintenance
Message-ID: <525057987.205844.1317314809935.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net>




To All, 



I?just wanted to see what?companies?other labs are?using for the maintenance of their equipment(processor, embedder, microtomes...). We are located in south jersey. 





Thanks for your help 

Jenny 



From Timothy.Morken <@t> ucsfmedctr.org  Thu Sep 29 11:48:45 2011
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy)
Date: Thu Sep 29 11:48:58 2011
Subject: [Histonet] Open - - Histology Supervisor, UC San Francisco
Message-ID: <8D7C2D242DBD45498006B21122072BF89448A249@MCINFRWEM003.ucsfmedicalcenter.org>

We have just opened the Histology Supervisor position at UC San Francisco. This is my current job. I am moving to a different supervisory position in electron microscopy.

If you want more info contact me privately

Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA

415.514.6042 (office)
415.885.7409 Fax
tim.morken@ucsfmedctr.org



Appy online at:

http://careers.ucsfmedicalcenter.org




2651



Job Title:



IPOX Lab Supervisor (HISTOTECHNOLOGIST, SUPVR)



Job Code:



9068



Department:



Pathology-Surgical / Histology



Location:

Parnassus



Full/Part Time:















Full-Time



Regular/Temporary:



Regular



Shift:



Not Applicable



Weekly Hours:



40



Union Information:





This classification is not represented by a union







Appointment Type: Career



At the University of California San Francisco Medical Center, teamwork and continual learning have maintained our top 10 ranking as one of "America's Best Hospitals" by U.S.News & World Report. For you, UCSF Medical Center is where you will share and discover something new every day with benefits and rewards that will last a lifetime.




Job Summary:
Under general direction from the Medical Director, the incumbent serves as supervisor to 3 Lead Histotechnologists and 9 Histotechnologists.   In addition to supervisory duties, the incumbent serves as a technical expert providing direction to staff as well as performing all technical aspects of surgical histology and immunohistochemistry procedures.  As supervisor:  recruits, hires, trains, completes performance evaluations, and resolves employee issues.  Provides orientation, completes competency assessments, maintains staff schedules, training and compliance documentation.  Implements new tests and procedures as requested by Medical Directors.  Updates and maintains the lab manuals and annual reviews required for accreditation.  Integrates new technologies into the laboratory to include analyzing and selecting of new equipment that meets space constraints and operational needs.  Ensures that new equipment and associated validation and staff training are performed.  Ensures that all equipment is well-maintained.  Ensures that staff training and documentation are completed as required.  Ensures that QC documentation is complete and quality standards are maintained in the laboratory.  Advises and assists researchers planning research projects and determines the ability of the laboratory to accommodate research projects, updating the Medical Director as required.  Other duties as assigned.




Required Qualifications:
College degree in a biological science, chemistry or a related field or equivalent education and work experience experience as a histotechnologist in a comparable high-volume hospital histology laboratory within the last seven years, including senior-level experience.  Demonstrated high-volume, high-quality sectioning and staining skills.  Ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines.  Excellent interpersonal and communication skills.   ASCP certification:  HT licensed or eligible required (candidate will be expected to obtain certification within 1 year), HTL desirable.  Excellent interpersonal communication skills required.  Demonstrated ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines required.  Previous, recent supervisory experience in a Histology and /or Immunoperoxidase Laboratory required.  Must be experienced with information technology systems and the use of hardware and software in business and laboratory settings.  Must be an effective user of spreadsheets and database software.
Preferred Qualifications:
ASCP certification:  HT or HTL-licensed strongly preferred.  Previous leadership experience in a hospital laboratory.
Required License/Certification:
N/A





From wbenton <@t> cua.md  Thu Sep 29 12:01:49 2011
From: wbenton <@t> cua.md (Walter Benton)
Date: Thu Sep 29 12:02:55 2011
Subject: [Histonet] Equipment maintenance
In-Reply-To: <525057987.205844.1317314809935.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net>
References: <525057987.205844.1317314809935.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net>
Message-ID: <0B8979A204680A42B93A52B486088CD9206C2042BE@CUAEXH1.GCU-MD.local>

 DolbeyJamison
Tech One
 BelAir Instrument Company

are all good companies, just make sure they are trained for your specific pieces of equipment.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wbenton@cua.md
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of zodiac29@comcast.net [zodiac29@comcast.net]
Sent: Thursday, September 29, 2011 12:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Equipment maintenance

To All,



I just wanted to see what companies other labs are using for the maintenance of their equipment(processor, embedder, microtomes...). We are located in south jersey.





Thanks for your help

Jenny



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law.  If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy.

From LSebree <@t> uwhealth.org  Thu Sep 29 12:05:30 2011
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Thu Sep 29 12:07:39 2011
Subject: [Histonet] IHC H.P. Negative Control
References: <20139929.229728.1317146267935.JavaMail.root@mail3d.brinkster.com>
Message-ID: 

One negative control per patient tissue and one positive tissue control per batch/run. 

Linda A. Sebree



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu on behalf of Adrienne Aperghis Kavanagh
Sent: Tue 9/27/2011 12:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC H.P. Negative Control
 
Hi Histonetters,

We would like to start to run up to 30 H.Pylori on our Benchmark daily.  Do GI labs that run H.P. on ALL stomach biopsies run a negative control for each patient tissue for H.P.?  I am just curious, as we have a 24 hour TAT, but the Benchmark only holds 30 slides.  What's everyone doing?

Thanks!

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From turkekul <@t> gmail.com  Thu Sep 29 12:10:58 2011
From: turkekul <@t> gmail.com (mesruh turkekul)
Date: Thu Sep 29 12:12:00 2011
Subject: [Histonet] Background in FFPE glandular tissue sections during IHC
Message-ID: 

Dear Histonetters,


When I do IHC on FFPE tissue sections. Most of time there is background due
to trapping of IgG/detection reagents probably in the connective tissue
fibers, mucins or other secretions of the glandular tissue. Especially
prostate and mammary gland. Any tips to get rid of that kind of background?
Do you know any treatment  to block mucins or connective tissue fibers from
 nonspecifically and electrostatically attracting IgGs and detection
reagents?

Regards,
Mesruh Turkekul
mskcc.org
From micropathlabs <@t> yahoo.com  Thu Sep 29 12:25:51 2011
From: micropathlabs <@t> yahoo.com (Sheila Haas)
Date: Thu Sep 29 12:25:57 2011
Subject: [Histonet] Fungus Control Tissue
Message-ID: <1317317151.99833.YahooMailNeo@web161719.mail.bf1.yahoo.com>

Hi all. Does anyone have a resource for control blocks? We are in need
of fungus controls and are trying to save the cost of buying?slides. 
Thanks all.
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
From joseph-galbraith <@t> uiowa.edu  Thu Sep 29 12:38:21 2011
From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe)
Date: Thu Sep 29 12:38:24 2011
Subject: [Histonet] Fungus Control Tissue
In-Reply-To: <1317317151.99833.YahooMailNeo@web161719.mail.bf1.yahoo.com>
References: <1317317151.99833.YahooMailNeo@web161719.mail.bf1.yahoo.com>
Message-ID: <6DC87DEA9229894DB3A09F8B61717A46106F4F05@hc-mailboxc1-n3.healthcare.uiowa.edu>

Sheila:

Have you tried your local or state animal disease lab or the National Animal Disease Lab in Ames, IA.  If you can work with animal instead of human tissue these labs may be able to help.  Do you have a large academic or tertiary care medical center nearby which has a block reclamation program (collects anonymized tissue blocks for research purposes that are being discarded by labs after the required holding period).  Sometimes these programs can find blocks with unusual or infectious processes as they collect from a broad range of sources.  (PS: We have such a program here.)

Joe

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Thursday, September 29, 2011 12:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fungus Control Tissue

Hi all. Does anyone have a resource for control blocks? We are in need
of fungus controls and are trying to save the cost of buying slides.
Thanks all.

Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


________________________________
Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.  If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.  Please reply to the sender that you have received the message in error, then delete it.  Thank you.
________________________________

From lblazek <@t> digestivespecialists.com  Thu Sep 29 12:45:56 2011
From: lblazek <@t> digestivespecialists.com (Blazek, Linda)
Date: Thu Sep 29 12:46:01 2011
Subject: [Histonet] Equipment maintenance
In-Reply-To: <525057987.205844.1317314809935.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net>
References: <525057987.205844.1317314809935.JavaMail.root@sz0062a.westchester.pa.mail.comcast.net>
Message-ID: <5A2BD13465E061429D6455C8D6B40E39127401B34F@IBMB7Exchange.digestivespecialists.com>

Marston Technical Services.  


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of zodiac29@comcast.net
Sent: Thursday, September 29, 2011 12:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Equipment maintenance




To All, 



I?just wanted to see what?companies?other labs are?using for the maintenance of their equipment(processor, embedder, microtomes...). We are located in south jersey. 





Thanks for your help 

Jenny 



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From jsantiago <@t> bellsouth.net  Thu Sep 29 13:07:37 2011
From: jsantiago <@t> bellsouth.net (Jerry Santiago)
Date: Thu Sep 29 13:07:48 2011
Subject: [Histonet] Passing of Jerry Meade
Message-ID: <660716.15068.qm@smtp118-mob.biz.mail.gq1.yahoo.com>

In case it has not been posted. Jerry Meade charter member for NSH has passed away. He attended this past symposium and expressed not feeling well. His death is attributed to heart failure.

He will be missed.

Rest in Peace.

-- Sent from my Palm Pre

From liz <@t> premierlab.com  Thu Sep 29 13:09:54 2011
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Thu Sep 29 13:11:23 2011
Subject: [Histonet] Passing of Jerry Meade
In-Reply-To: <660716.15068.qm@smtp118-mob.biz.mail.gq1.yahoo.com>
Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA0AD5@SBS2K8.premierlab.local>

That's so sad.  I have known Jerry since I got in involved in the MSH state society back in the early 80's.  He will be missed.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Santiago
Sent: Thursday, September 29, 2011 12:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Passing of Jerry Meade

In case it has not been posted. Jerry Meade charter member for NSH has passed away. He attended this past symposium and expressed not feeling well. His death is attributed to heart failure.

He will be missed.

Rest in Peace.

-- Sent from my Palm Pre

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From brian <@t> prometheushealthcare.com  Thu Sep 29 13:14:51 2011
From: brian <@t> prometheushealthcare.com (Brian- Prometheus)
Date: Thu Sep 29 13:15:01 2011
Subject: [Histonet] Top notch histotech needed in TN
Message-ID: <003201cc7ed3$b1c15b20$15441160$@com>

 

Position with a regional diagnostic lab in Maryville, TN

 

Day shift opening

 

M-F occasional Saturday

 

Will be doing IHC, cutting, embedding, general histology work

 

Salary competitive for the right person

 

Someone extremely efficient, good personality

 

ASCP preferred

 

Please contact me today for immediate consideration!

 

 

Brian Feldman

Principal

Prometheus Healthcare 

Office 301-693-9057

Fax 301-368-2478

 
 brian@prometheushealthcare.com

  www.prometheushealthcare.com

*** Stay up to date on the newest positions and healthcare trends nationwide
on Twitter!***

   http://twitter.com/PrometheusBlog

 

 

 

From micropathlabs <@t> yahoo.com  Thu Sep 29 13:17:30 2011
From: micropathlabs <@t> yahoo.com (Sheila Haas)
Date: Thu Sep 29 13:17:35 2011
Subject: [Histonet] Fungus Control Tissue
In-Reply-To: <1317317151.99833.YahooMailNeo@web161719.mail.bf1.yahoo.com>
References: <1317317151.99833.YahooMailNeo@web161719.mail.bf1.yahoo.com>
Message-ID: <1317320250.72347.YahooMailNeo@web161713.mail.bf1.yahoo.com>

Guess I should have included that we're an AP lab without
access to a micro department. Sorry for the lack of info.
Thanks all.

Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
?

From: Sheila Haas 
To: "histonet@lists.utsouthwestern.edu" 
Sent: Thursday, September 29, 2011 1:25 PM
Subject: [Histonet] Fungus Control Tissue

Hi all. Does anyone have a resource for control blocks? We are in need
of fungus controls and are trying to save the cost of buying?slides. 
Thanks all.
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From foreightl <@t> gmail.com  Thu Sep 29 13:19:43 2011
From: foreightl <@t> gmail.com (Patrick Laurie)
Date: Thu Sep 29 13:19:47 2011
Subject: [Histonet] Fungus Control Tissue
In-Reply-To: <6DC87DEA9229894DB3A09F8B61717A46106F4F05@hc-mailboxc1-n3.healthcare.uiowa.edu>
References: <1317317151.99833.YahooMailNeo@web161719.mail.bf1.yahoo.com>
	<6DC87DEA9229894DB3A09F8B61717A46106F4F05@hc-mailboxc1-n3.healthcare.uiowa.edu>
Message-ID: 

We took some fresh lung, added some cream cheese which got really moldy,
waited a couple of days, and then processed it.  We got a great control with
some form of aspergillus on the lung surface.

On Thu, Sep 29, 2011 at 10:38 AM, Galbraith, Joe  wrote:

> Sheila:
>
> Have you tried your local or state animal disease lab or the National
> Animal Disease Lab in Ames, IA.  If you can work with animal instead of
> human tissue these labs may be able to help.  Do you have a large academic
> or tertiary care medical center nearby which has a block reclamation program
> (collects anonymized tissue blocks for research purposes that are being
> discarded by labs after the required holding period).  Sometimes these
> programs can find blocks with unusual or infectious processes as they
> collect from a broad range of sources.  (PS: We have such a program here.)
>
> Joe
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:
> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
> Sent: Thursday, September 29, 2011 12:26 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Fungus Control Tissue
>
> Hi all. Does anyone have a resource for control blocks? We are in need
> of fungus controls and are trying to save the cost of buying slides.
> Thanks all.
>
> Sheila Haas
> Laboratory Supervisor
> MicroPath Laboratories, Inc.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ________________________________
> Notice: This UI Health Care e-mail (including attachments) is covered by
> the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is
> confidential and may be legally privileged.  If you are not the intended
> recipient, you are hereby notified that any retention, dissemination,
> distribution, or copying of this communication is strictly prohibited.
>  Please reply to the sender that you have received the message in error,
> then delete it.  Thank you.
> ________________________________
>
> _______________________________________________
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie@cellnetix.com
From PAMarcum <@t> uams.edu  Thu Sep 29 13:23:07 2011
From: PAMarcum <@t> uams.edu (Marcum, Pamela A)
Date: Thu Sep 29 13:23:56 2011
Subject: [Histonet] AFB Controls
Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3209697449@Mail2Node2.ad.uams.edu>

Does anyone know where we can get a good AFB control?  Ours have exhausted and we can't find a case here with a positive reading.

Best Regards,

Pamela A Marcum
UAMS
Slot 502
4301 W Markham Street
Little Rock AR 72205
Office: 501-686-7554
Fax: 501-686-7151

Confidentiality Notice: This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain
confidential and privileged information.  Any unauthorized review,
use, disclosure or distribution is prohibited.  If you are not the 
intended recipient, please contact the sender by reply
e-mail and destroy all copies of the original message..
From relia1 <@t> earthlink.net  Thu Sep 29 13:24:34 2011
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Thu Sep 29 13:24:37 2011
Subject: [Histonet] Jerry Meade
Message-ID: 

I am so sorry to hear of Jerry's passing.  I too have known him for many
years.  I regret that I didn't get a chance to see him at NSH.
RIP Jerry.

Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.comPamBarkerRELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

From micropathlabs <@t> yahoo.com  Thu Sep 29 13:35:41 2011
From: micropathlabs <@t> yahoo.com (Sheila Haas)
Date: Thu Sep 29 13:35:45 2011
Subject: [Histonet] Thanks
Message-ID: <1317321341.2563.YahooMailNeo@web161701.mail.bf1.yahoo.com>

Thanks everyone. We'll start growing our own?shortly. 
Thanks for the great ideas!
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
From tnsbull <@t> gmail.com  Thu Sep 29 14:44:01 2011
From: tnsbull <@t> gmail.com (Terry&Star Bullard)
Date: Thu Sep 29 14:44:05 2011
Subject: [Histonet] KP Markers!
Message-ID: 

From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M.
Gorham
Sent: Tuesday, September 27, 2011 5:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] KP Markers

I received a call from Mercedes Medical saying they can not get the KP
Markers so many of us like.  Does any one have another source for these
wonderful pens?  Thanks and have a wonderful evening, Kathy Gorham H.T.


Kathy,
I also have been looking for KP markers. I contacted Mercedes Medical and
they told me that these markers are no longer being sold because of the
amount of returns and issues they have had in the past year from their
customers. Apparently, the formula was changed and the company overseas that
makes them have not changed the formula back, so Mercedes Medical is no
longer purchasing them. I believe these markers were the best ones on the
market before the formula change. I'm hoping the foreign company decides to
make them as they used to and scrap this new formula!

Star Bullard HT(ASCP)
From b-shmaltsuyev <@t> northwestern.edu  Thu Sep 29 15:30:05 2011
From: b-shmaltsuyev <@t> northwestern.edu (Bella Shmaltsuyeva)
Date: Thu Sep 29 15:30:37 2011
Subject: [Histonet] Immunohistochemistry TLR4 on mouse tissue
Message-ID: 

Hello,



I'm new to the histo listserv. I'm having problems with stain TLR4 on paraffin embedded mouse tissue. Does anyone have experience with this particular immuno stain? Please provide the company name and catalog number.



Thanks,



Bella Shmaltsuyev




From asandoval <@t> pathlogic.com  Thu Sep 29 15:55:02 2011
From: asandoval <@t> pathlogic.com (Anthony Sandoval)
Date: Thu Sep 29 15:57:24 2011
Subject: [Histonet] Searching for Histotechs
Message-ID: <37C1EF69DB7C7147B5DDC946F4A1A5003DE12F@PATHEXCH001.Path.local>

Path Logic in Carmichael California (near Sacramento) is currently looking for histotechs to work the 2nd and 3rd shifts. We offer medical, dental, vision, and a 401(K) plan. If interested please email your resume to asandoval@pathlogic.com.


From Erik.Dokken <@t> onassignment.com  Thu Sep 29 19:13:21 2011
From: Erik.Dokken <@t> onassignment.com (Erik Dokken)
Date: Thu Sep 29 19:13:28 2011
Subject: [Histonet] Contract to Hire Opportunity for Histotech in CA
In-Reply-To: <201109201701.p8KGveLF015202@smtp11.onasgn.net>
References: <201109201701.p8KGveLF015202@smtp11.onasgn.net>
Message-ID: 

On Assignment Healthcare has a contract to hire opportunity for a Histotech on the SF Peninsula.

This position will start ASAP and is full time.

Employees will be eligible for benefits upon completion of 30 days of work.

Competitive pay and referral bonus available.

If you would like to hear more contact On Assignment Healthcare today at 650-866-3386.


Erik Dokken
Area Manager - No. CA, WA, and TX
On Assignment, Inc. Local Allied Healthcare


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Tuesday, September 20, 2011 10:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 94, Issue 23

Send Histonet mailing list submissions to
        histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
        http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
        histonet-request@lists.utsouthwestern.edu

You can reach the person managing the list at
        histonet-owner@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Gary Steinke is out of the office (Gary_Steinke@vwr.com)
   2. Ken Marissael is out of the office (Ken_Marissael@vwr.com)
   3. Histotech needed (Joyce Cline)
   4. Diane Tokugawa/CA/KAIPERM is out of the office.
      (Diane.Tokugawa@kp.org)
   5. How to store paraffin blocks ? (Itai Moshe)
   6. SV: [Histonet] How to store paraffin blocks ?
      (Erikstad, Gudrun Hovstein)
   7. Verification System (Ann Angelo)
   8. Re: How to store paraffin blocks ? (Rene J Buesa)
   9. RE: How to store paraffin blocks ? (Sherwood, Margaret)
  10. Tissue handling at embedding (Morken, Timothy)
  11. Uni-trieve, antigen retrieval for immunofluorescence
      (Andrea Marion)


----------------------------------------------------------------------

Message: 1
Date: Mon, 19 Sep 2011 14:02:34 -0400
From: Gary_Steinke@vwr.com
Subject: [Histonet] Gary Steinke is out of the office
To: histonet@lists.utsouthwestern.edu
Message-ID:
        
Content-Type: text/plain; charset=US-ASCII


I will be out of the office starting  09/19/2011 and will not return until 09/26/2011.

I will be out of the office from September 19th and returning on September 26th.  I will out of town attending a business meeting and will have very limited access to email and voice mail.  If you need assistance immediately, please contact Customer Service at 877-881-1192.  Take care and have a great day.




------------------------------

Message: 2
Date: Mon, 19 Sep 2011 14:03:30 -0400
From: Ken_Marissael@vwr.com
Subject: [Histonet] Ken Marissael is out of the office
To: histonet@lists.utsouthwestern.edu
Message-ID:
        
Content-Type: text/plain; charset=US-ASCII


I will be out of the office starting  09/19/2011 and will not return until 09/23/2011.

I will be away on 9/16 and return 9/27. I will have limited e-mail and cell phone access, but will try to get back to you as quickly as possible. While I am away, please contact VWR Healthcare Customer Service at 877-881-1192.




------------------------------

Message: 3
Date: Mon, 19 Sep 2011 14:19:16 -0400
From: Joyce Cline 
Subject: [Histonet] Histotech needed
To: "histonet@lists.utsouthwestern.edu"
        
Message-ID:
        
Content-Type: text/plain; charset="iso-8859-1"

Full time position, M-F varying shifts 6:00am to 6:00pm, rotating bench assignments, IHC, special stains and autopsies. No Saturdays or week end call.

We are near Wash D.C., Baltimore and other site seeing areas.

Go to Meritushealth.com HR department for job listings.

Joyce Cline, H. T. (ASCP)
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
joyce.cline@meritushealth.com

________________________________
***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system.


------------------------------

Message: 4
Date: Mon, 19 Sep 2011 16:25:31 -0700
From: Diane.Tokugawa@kp.org
Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.
To: histonet@lists.utsouthwestern.edu
Message-ID:
        
Content-Type: text/plain; charset=US-ASCII


I will be out of the office starting  09/19/2011 and will not return until 09/21/2011.

Note:   For Cytology issues, please call Molly  at 8-421-5487,  Eric at
8-421-5405, or Wanda 8-421-5426   For Histology issues, please call Mario
at 8-421-4961, general histology lab 8-421- 5408 or Wanda at 8-421-5426.

------------------------------

Message: 5
Date: Tue, 20 Sep 2011 09:23:04 +0300
From: Itai Moshe 
Subject: [Histonet] How to store paraffin blocks ?
To: histonet@lists.utsouthwestern.edu
Message-ID:
        
Content-Type: text/plain; charset=ISO-8859-1

Hi Histonet's,

What is the recommended way to store paraffin blocks for a long period ?
Room temperature, or 4C ?

The advantage in storing at 4C is that the blocks are always ready for sectioning.
Is there a risk when storing at 4C that the humidity in the fridge will cause damage to the paraffin blocks and the tissue ?
After sectioning, How do you store the slides, and for how long ?


Thank's
Itai Moshe


------------------------------

Message: 6
Date: Tue, 20 Sep 2011 08:55:28 +0200
From: "Erikstad, Gudrun Hovstein" 
Subject: SV: [Histonet] How to store paraffin blocks ?
To: 
Message-ID:
        

Content-Type: text/plain;       charset="iso-8859-1"


 Hi,

We store paraffin blocks and stained slides at room temperature in rooms without windows, so there is no sunlight.
We store blocks and slides "forever". We have paraffin blocks from before 1930.
"Room temperature" in Norway is usually about 20 degrees Celcius.

Some stained slides will fade with time (silver techniques, immuno), but the routine HE usually last for many, many years.
After we excanged Xylene with a substitute (Tissue-Clear) four years ago, we had some "bleeding" and fading of the HE-stains, but after taking more care changing the Xylene substitute in the clearing step more often this is no more a problem.
The paraffin blocks keep very well. We frequently do section and stain as well as do molecular techniques (PCR, In situ) on material from the 1970'ies and before, and it's remarkable how well the formalin fixed, paraffin embedded tissue keep.

I would not keep the blocks in a fridge, as you say, the humidity in the fridge is quite high.
The time to cool a block from room temperature to suitable for sectioning is only about two minutes.
Now, because Norway has a relatively cold climate, the air humidity is also quite low compared to warmer climates, even if it rains here ALL the time... ;-) Heat and high humidity might affect your blocks.

Gudrun H. Erikstad, Trondheim, Norway



-----Opprinnelig melding-----
Fra: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] P? vegne av Itai Moshe
Sendt: 20. september 2011 08:23
Til: histonet@lists.utsouthwestern.edu
Emne: [Histonet] How to store paraffin blocks ?

Hi Histonet's,

What is the recommended way to store paraffin blocks for a long period ?
Room temperature, or 4C ?

The advantage in storing at 4C is that the blocks are always ready for sectioning.
Is there a risk when storing at 4C that the humidity in the fridge will cause damage to the paraffin blocks and the tissue ?
After sectioning, How do you store the slides, and for how long ?


Thank's
Itai Moshe
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 7
Date: Tue, 20 Sep 2011 10:09:41 -0400 (EDT)
From: Ann Angelo 
Subject: [Histonet] Verification System
To: histonet@lists.utsouthwestern.edu
Message-ID: <8CE45B6132A22E2-7D8-725E9@webmail-d167.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"


I am looking for a verification system which does not require the data entry to be performed prior to cutting (ruling out Vantage and Cerebro).  I have looked at Thermo's slidemate but can't get them to move fast enough.
Does anyone know of another verification system that I may be able to try which does not require the data entry to be performed prior to microtomy?  Thanks, Ann





------------------------------

Message: 8
Date: Tue, 20 Sep 2011 07:19:47 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] How to store paraffin blocks ?
To: histonet@lists.utsouthwestern.edu, Itai Moshe
        
Message-ID:
        <1316528387.5562.YahooMailClassic@web65706.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

The usual procedure is to store at room temperature. It is assumed that the room is air conditioned, otherwise if the temp is above 80?F some blocks may stick together, although that is not a real problem.
If at 4?C you will need a refrigerated room or a series of refrigerators, which is an additional and unnecessary expenditure.
We use to store the blocks for 9 years, unless it is a special case (used in teaching) and those are stored forever.
Supposedly you use the slides for some procedure and those cut as "extras" are kept along with the originals stained in the same files.
If you are referring to sections for IHC or other procedure, the epitopes will be oxidized by the oxygen in the air in less than 2 weeks, so they can either be stored in a fridge, or covered with melted paraffin, or stored in mineral oil.
Ren? J.

--- On Tue, 9/20/11, Itai Moshe  wrote:


From: Itai Moshe 
Subject: [Histonet] How to store paraffin blocks ?
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 20, 2011, 2:23 AM


Hi Histonet's,

What is the recommended way to store paraffin blocks for a long period ?
Room temperature, or 4C ?

The advantage in storing at 4C is that the blocks are always ready for sectioning.
Is there a risk when storing at 4C that the humidity in the fridge will cause damage to the paraffin blocks and the tissue ?
After sectioning, How do you store the slides, and for how long ?


Thank's
Itai Moshe
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 9
Date: Tue, 20 Sep 2011 10:58:52 -0400
From: "Sherwood, Margaret" 
Subject: RE: [Histonet] How to store paraffin blocks ?
To: "Rene J Buesa" ,
        ,    "Itai Moshe"
        
Message-ID:
        <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A01@PHSXMB30.partners.org>
Content-Type: text/plain; charset="iso-8859-1"

We are a research group, but had paraffin blocks stored at room temperature for
20+ years.  We finally made the decision to just keep 2 years in the lab
20+ and
store 5 years off-site.  We trashed the rest.

Peggy


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, September 20, 2011 10:20 AM
To: histonet@lists.utsouthwestern.edu; Itai Moshe
Subject: Re: [Histonet] How to store paraffin blocks ?

The usual procedure is to store at room temperature. It is assumed that the room is air conditioned, otherwise if the temp is above 80?F some blocks may stick together, although that is not a real problem.
If at 4?C you will need a refrigerated room or a series of refrigerators, which is an additional and unnecessary expenditure.
We use to store the blocks for 9 years, unless it is a special case (used in
teaching) and those are stored forever.
Supposedly you use the slides for some procedure and those cut as "extras" are kept along with the originals stained in the same files.
If you are referring to sections for IHC or other procedure, the epitopes will be oxidized by the oxygen in the air in less than 2 weeks, so they can either be stored in a fridge, or covered with melted paraffin, or stored in mineral oil.
Ren? J.

--- On Tue, 9/20/11, Itai Moshe  wrote:


From: Itai Moshe 
Subject: [Histonet] How to store paraffin blocks ?
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 20, 2011, 2:23 AM


Hi Histonet's,

What is the recommended way to store paraffin blocks for a long period ?
Room temperature, or 4C ?

The advantage in storing at 4C is that the blocks are always ready for sectioning.
Is there a risk when storing at 4C that the humidity in the fridge will cause damage to the paraffin blocks and the tissue ?
After sectioning, How do you store the slides, and for how long ?


Thank's
Itai Moshe
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.




------------------------------

Message: 10
Date: Tue, 20 Sep 2011 09:44:26 -0700
From: "Morken, Timothy" 
Subject: [Histonet] Tissue handling at embedding
To: "histonet@lists.utsouthwestern.edu"
        
Message-ID:
        <8D7C2D242DBD45498006B21122072BF85E2AEE21@MCINFRWEM003.ucsfmedicalcenter.org>

Content-Type: text/plain; charset=us-ascii

What procedures do you have in place to prevent tissue loss at embedding? What do you do if tissue appears to have been lost?

And do you clean embedding molds before each reuse, or after one-days use (which may be many re-uses)?


Thanks for all info!


Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA

415.514.6042 (office)
415.885.7409 Fax
tim.morken@ucsfmedctr.org




------------------------------

Message: 11
Date: Tue, 20 Sep 2011 11:59:19 -0500
From: "Andrea Marion" 
Subject: [Histonet] Uni-trieve, antigen retrieval for
        immunofluorescence
To: histonet@lists.utsouthwestern.edu
Message-ID:
        <6623612296b9078e4db4eccef8f88c74.squirrel@webmail.uic.edu>
Content-Type: text/plain;charset=iso-8859-1

Hello all,

Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported to be a universal antigen retrieval solution that can be used at lower temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear
antigens):

http://innvx.com/unitrievepage.html

The company claims that the reagent is a universal retrieval solution for all antibodies and tissues (which is silly of course - how could they know?). Does anyone have any experience with the product?

I am interested because I see that increased heat during antigen retrieval causes greater tissue autofluorescence during immunofluorescence stainings. My current protocol is to use 20 minutes at 90-95 C on a hot plate using sodium citrate buffer. Does anyone else either Uni-trieve or a different reagent/protocol for immunofluorescence stainings?

Thanks,

Andrea

Andrea Marion
Graduate Student
University of Illinois at Chicago




------------------------------

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 94, Issue 23
****************************************

From W.E.J.Hoekert <@t> olvg.nl  Fri Sep 30 03:37:55 2011
From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.)
Date: Fri Sep 30 03:38:05 2011
Subject: [Histonet] P 16
References: <1317237291.78880.YahooMailNeo@web43513.mail.sp1.yahoo.com>
Message-ID: <1190CB05C44B13409483514729C2FC3601F841D4@PAIT42.olvg.nl>

Hi Phyllis,
 
We are doing a double stain (Mib-1 - P16) on cervix biopsies in which the P16 antibody is coloured with AP. It works fine, our pathologists are quite fond of it. Almost every day we have a few slides.
 
Willem Hoekert
OLVG, Amsterdam
The Netherlands
 
 
 

________________________________

Van: histonet-bounces@lists.utsouthwestern.edu namens Phyllis Thaxton
Verzonden: wo 28-9-2011 21:14
Aan: Histonet@lists.utsouthwestern.edu
Onderwerp: [Histonet] P 16



Has anyone ever used P16 with AP Red detection on the Ventana Benchmark?


Phyllis Jordan HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Disclaimer: 

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In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend.


From SteveM <@t> mcclainlab.com  Fri Sep 30 08:35:08 2011
From: SteveM <@t> mcclainlab.com (Steve McClain)
Date: Fri Sep 30 08:22:17 2011
Subject: [Histonet] RE: Histonet Digest, Vol 94,
	Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons
	e.g., Slide Bright, down the drain?
Message-ID: 

The argument against using toxic, yet recyclable xylene in favor of a
more expensive, less efficacious xylene-substitute like Slide Bright in
larger labs is not compelling.

Aside from low volatility of Slide-Bright, what is gained in using a
more expensive substitute whose toxicities are not well-known?

The chemical composition differs, yet most MSDS warning are the same.

The advantage for low volatility can be an advantage for small hole in
the wall lab settings, like an office Mohs lab or frozen section lab in
the OR suite where ventilation may be an issue.

 

Unlike xylene where 75% recycling yield is norm , Slide-Bright can be
recycled- how well, I don't know.

I've requested specifics from B/R for a protocol and will forward that
info later.

However, if Slide-Bright is disposed like xylene it carries the same
disposal cost.

The company indicates Slide-Bright is a flammable aliphatic hydrocarbon
which laden with paraffin may be disposed of down the drain with copious
amounts of water, yet it is the lab directors job to ensure all local
state and federal guidelines are followed.

Aren't you defeating part of your purpose in working toward a safer lab
and greener environment by dumping aliphatic hydrocarbons into your
ground water?

Steve

Steve A. McClain, MD

McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000

 

Slide-Bright MSDS follows:

Revision Date: 6/1/2006

MATERIAL SAFETY DATA SHEET

Conforms to 93/112/EC and ISO 11014-1

 

1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION

PRODUCT NAME:    OptiClear E
PRODUCT NUMBER:   OE-104

CHEMICAL NAMES/ DESCRIPTION:

Aliphatic Hydrocarbon

 

MANUFACTURER: National Diagnostics, Inc.

305 Patton Drive

Atlanta, GA 30336

TELEPHONE NUMBER: (800) 526-3867 (404) 699-2121

EMERGENCY NUMBER:

CHEMTREC (800) 424-9300

2. COMPOSITION / INFORMATION ON INGREDIENTS

 

Component                                                        % Comp
CAS #    EINECS #              TLV (units)

 

Aliphatic Hydrocarbons

 

EEC LABEL SYMBOL AND CLASSIFICATION

 

R:

 

 

 

 

11-38

 

100

 

400 ppm

Highly flammable.  Irritating to skin.

S:  (2-) 16-23-24-62

Keep out of the reach of children.  Keep away from sources of ignition -
No Smoking.  Do not breathe fumes.  Avoid contact with the skin.  If
swallowed, do not induce vomiting:  Seek medical advice immediately and
show this container or label.

 

 

 

3. HAZARDS IDENTIFICATION

APPEARANCE AND ODOR:      Clear, colorless liquid

 

EMERGENCY OVERVIEW - IMMEDIATE HAZARD

HIGHLY FLAMMABLE. PRODUCT IS SLIGHTLY IRRITATING TO EYES (NO INJURY).
HIGH VAPOR MAY CAUSE RESPIRATORY TRACT IRRITATION, HEADACHE, DIZZINESS,
ANESTHESIA, DROWSINESS, UNCONSCIOUSNESS, OR DEATH.  INGESTION: MINIMAL
TOXICITY.  ASPIRATION MAY LEAD TO PULMONARY INJURY AND DEATH.

EMERGENCY OVERVIEW - CHRONIC HAZARD W ARNING

 

NO CHRONIC HAZARDS SUSPECTED.

 

POTENTIAL HEALTH EFFECTS

INHALATION

 

High vapor/aerosol concentrations (greater than approximately 1000 ppm)
are irritating to the eyes and the respiratory tract, may cause
headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other
central nervous system effects, including death.

INGESTION

 

Minimal toxicity by ingestion, though small amounts of this product
aspirated into the respiratory system durin ingestion or vomiting may
cause mild to severe pulmonary injury, possibly progressing to death.

SKIN

 

Low order or toxicity.  Frequent or prolonged contact may irritate and
cause dermatitis.  Skin contact may aggravate an existing dermatitis
condition.

EYES

 

Slightly irritating but does not injure eye tissue.

 

SIGNS AND SYMPTOMS 0F OVEREXPOSURE

INHALATION

 

Page 1
OptiClear E

Headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other
central nervous system effects, including death.

INGESTION

 

Minimal toxicity by ingestion. SKIN

Dermatitis may occur with frequent or prolonged contact. EYES

Product is only slightly irritating to eye tissue, non injurious.
CARCINOGENICITY

Not listed by NTP, IARC, or OSHA. MUTAGENICITY

No information available. REPRODUCTIVE TOXICITY

No information available. TERATOGENIC EFFECTS

No information available. ROUTES OF ENTRY

Inhalation or by skin contact. TARGET ORGAN STATEMENT

No information available.

 

4. FIRST AID MEASURES

INHALATION:

Remove to fresh air.  If not breathing, give artificial respiration.  If
breathing is difficult, give oxygen.  Call a physicia

 

 

INGESTION:

Do not induce vomiting.  If swallowed and the person is conscious,
immediately give large amounts of water.  Get medical attention.

 

SKIN:

Immediately flush skin with plenty of soap and water for at least 15
minutes while removing contaminated clothing and shoes.  Get medical
attention.  Wash clothing before reuse.  Thoroughly clean shoes before
reuse.

 

EYES:

Immediately flush eyes with plenty of water for at least fifteen
minutes, lifting lower and upper eyelids occasionally. Get medical
attention immediately.

 

5. FIRE FIGHTING MEASURES

FLASH POINT:

 

FLASH POINT METHOD:

45F (7C)

 

TCC

FLAMMABLE LIMITS:

 

AUTOIGNITION TEMPERATURE:

LEL: 1.3%; UEL: 10.2% @ 77 F

 

720 F

 

EXTINGUISHING MEDIA

Dry powder, foam, carbon dioxide. (W ater may be ineffective.)

 

 

PROTECTIVE EQUIPMENT

In the event of a fire, wear full protective clothing and NIOSH-approved
self-contained breathing apparatus with full facepiece operated in the
pressure demand or other positive pressure mode.

 

 

 

 

Page 2
OptiClear E

HAZARDOUS COMBUSTION PRODUCTS:

Thermal decomposition products may include carbon monoxide, carbon
dioxide, and hydrocarbons.

 

 

UNUSUAL FIRE AND EXPLOSION HAZARDS

Flammable liquid.  Vapor forms explosive mixtures with air.  Vapor may
travel considerable distances to ignition source and flash back.

 

NFPA CODES: Health:  1

 

Flammability: 3

 

Reactivity:   0

 

6. ACCIDENTAL RELEASE MEASURES

STEPS TO BE TAKEN IN CASE MATERIAL IS RELEASED OR SPILLED

Eliminate source of ignition.  Ventilate area.  Cover with absorbent
material (soda ash) to confine spill and sweep or shovel into container.
Close container tightly.  Avoid breathing vapors.

 

W ASTE DISPOSAL METHOD

Disposal must be made in accordance with applicable federal, state, and
local regulations.

 

 

PERSONAL PRECAUTIONS

Wear appropriate protective equipment as specified in section 8.

 

 

 

7. HANDLING AND STORAGE

HANDLING

Avoid contact and inhalation.  Do not get in eyes, on skin, on clothing.
W ash thoroughly after handling.  Transfer methods should avoid static
sparks.  Use explosion proof ventilation.

 

STORAGE

Keep in a tightly closed container, stored in a cooled, dry, ventilated
area away from sources of heat or ignition. Protect from physical
damage.  Isolate from incompatible materials (section 10).

 

STORAGE TEMPERATURE:

Room Temperature

 

DISPOSAL

Observe all national, state, and local regulations regarding product
disposal.  Containers of this material may be hazardous when empty since
they retain product residues (vapors, liquids).

 

8. EXPOSURE CONTROLS / PERSONAL PROTECTION

AIRBORNE EXPOSURE LIMITS:

Component:

Aliphatic Hydrocarbons

ACGIH Threshold Lim it Value (TLV): OSHA Permissible Exposure Lim it
(PEL):

ENGINEERING CONTROLS

400 ppm

A system of local and/or general exhaust is recommended to keep employee
exposures below the Airborn Exposure Limits.  Local exhaust ventilation
is generally preferred because it can control the emissions of the
contaminant at it source.

RESPIRATORY PROTECTION

If the exposure limit is exceeded, wear a supplied air, full-facepiece
respirator, airlined hood, or full-facepiece self- contained breathing
apparatus.

 

 

EYE PROTECTION Safety glasses.

 

 

SKIN PROTECTION

Wear protective gloves and clean body covering clothing.

 

 

 

 

 

 

 

Page 3
OptiClear E

OTHER CONTROL MEASURES

Use explosion-proof ventilation equipment.  Special care is required
given the highly flammable nature of this material.

 

9. PHYSICAL PROPERTIES

Boiling Point

 

Melting Point

 

Vapor Pressure

mm Hg

Vapor Density

Air = 1

% Volatile by Volume

244 - 284 F Less than -76

36 mmHg @ 68 F

 

3.9

 

100

Evaporation Rate

 

Solubility in W ater pH

Specific Gravity

(H20 = 1)

1.6 (n-Bu Acetate=1) Less than 0.01% @77F

N.A.

 

0.72 @ 60 F

 

10. STABILITY AND REACTIVITY

STABILITY

Stable under normal conditions of use.

 

CONDITIONS TO AVOID Heat, sources of ignition.

HAZARDOUS DECOMPOSITION PRODUCTS None

HAZARDOUS POLYMERIZATION Will not occur

 

 

INCOMPATIBLES

 

Strong oxidizing agents.

 

 

 

11. TOXICOLOGICAL INFORMATION

PRODUCT LD50 VALUES

OptiClear E

Oral Rat LD50 (m g/kg):                 Minimal toxicity by ingestion.

OptiClear E

Derm al Rabbit LD50 (mg/kg):

Minimal toxicity by skin contact.

 

COMPONENT CANCER LIST STATUS

 

Component

 

 

NTP Carcinogen

Known               Anticipated

 

 

 

IARC Category

Aliphatic Hydrocarbons

 

12. ECOLOGICAL INFORMATION

No                         No                       None

 

No specific ecological data is available for this material.  Please
refer to Section 6 for information regarding accidental releases.

13. DISPOSAL CONSIDERATIONS

Observe all national, state, and local regulations regarding product
disposal.  Containers of this material may be hazardou when empty since
they retain product residues (vapors, liquids).

 

14. TRANSPORT INFORMATION

D.O.T.

Proper Shipping Name Petroleum Products N.O.S.

 

 

Hazard Class: 3

UN Num ber: 1268

Packing Group  3

 

 

I.A.T.A.

 

 

Page 4
OptiClear E

Proper Shipping Name Petroleum Products N.O.S.

 

 

Hazard Class: 3

UN Num ber: 1268

Packing Group  3

 

 

I.M.O.

Proper Shipping Name Petroleum Products N.O.S.

 

Hazard Class: 3

UN Num ber: 1268

Packing Group  3

 

15. REGULATORY INFORMATION

UNITED STATES

TSCA Regulatory:

All intentional ingredients are listed on the TSCA Inventory.

 

SARA 311/312 Hazard Categories

Component

 

 

Fire         Pressure    Reactivity      Acute         Chronic

 

Aliphatic Hydrocarbons

EUROPE

EEC Regulatory:

 

Yes               No               No               No               No

All intentional ingredients are listed on the European EINECS Inventory.

 

EEC LABEL SYMBOL AND CLASSIFICATION

 

R:

 

 

11-38

Highly flammable.  Irritating to skin.

S:  (2-) 16-23-24-62

Keep out of the reach of children.  Keep away from sources of ignition -
No Smoking.  Do not breathe fumes.  Avoid contact with the skin.  If
swallowed, do not induce vomiting:  Seek medical advice immediately and
show this container or label.

 

 

 

16. OTHER INFORMATION

NFPA CODES: Health:  1

Flammability: 3

Reactivity:   0

 

MANUFACTURER DISCLAIMER: The information given herein is offered in good
faith as accurate, but without guarantee.  Conditions of use and
suitability of the product for particular uses are beyond our control.
All risks of use of the product are therefore assumed by the user.
Nothing is intended as a recommendation for uses which infringe valid
patents or as extending license under valid patents.  Appropriate
warnings and safe handling procedures should be provided to handlers and
users.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Page 5
OptiClear E

 

From ryaskovich <@t> dir.nidcr.nih.gov  Fri Sep 30 09:06:11 2011
From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E])
Date: Fri Sep 30 09:06:53 2011
Subject: [Histonet] RE: Histonet Digest, Vol 94,	Issue 38 Xylene safe
	disposal vs. aliphatic hydrocarbons	e.g., Slide Bright, down the drain?
In-Reply-To: 
References: 
Message-ID: 

Steve,
 I totally agree. NOTHING but water should go down the drain.
Ruth
N.I.H.

-----Original Message-----
From: Steve McClain [mailto:SteveM@mcclainlab.com] 
Sent: Friday, September 30, 2011 9:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

The argument against using toxic, yet recyclable xylene in favor of a
more expensive, less efficacious xylene-substitute like Slide Bright in
larger labs is not compelling.

Aside from low volatility of Slide-Bright, what is gained in using a
more expensive substitute whose toxicities are not well-known?

The chemical composition differs, yet most MSDS warning are the same.

The advantage for low volatility can be an advantage for small hole in
the wall lab settings, like an office Mohs lab or frozen section lab in
the OR suite where ventilation may be an issue.

 

Unlike xylene where 75% recycling yield is norm , Slide-Bright can be
recycled- how well, I don't know.

I've requested specifics from B/R for a protocol and will forward that
info later.

However, if Slide-Bright is disposed like xylene it carries the same
disposal cost.

The company indicates Slide-Bright is a flammable aliphatic hydrocarbon
which laden with paraffin may be disposed of down the drain with copious
amounts of water, yet it is the lab directors job to ensure all local
state and federal guidelines are followed.

Aren't you defeating part of your purpose in working toward a safer lab
and greener environment by dumping aliphatic hydrocarbons into your
ground water?

Steve

Steve A. McClain, MD

McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000

 

Slide-Bright MSDS follows:

Revision Date: 6/1/2006

MATERIAL SAFETY DATA SHEET

Conforms to 93/112/EC and ISO 11014-1

 

1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION

PRODUCT NAME:    OptiClear E
PRODUCT NUMBER:   OE-104

CHEMICAL NAMES/ DESCRIPTION:

Aliphatic Hydrocarbon

 

MANUFACTURER: National Diagnostics, Inc.

305 Patton Drive

Atlanta, GA 30336

TELEPHONE NUMBER: (800) 526-3867 (404) 699-2121

EMERGENCY NUMBER:

CHEMTREC (800) 424-9300

2. COMPOSITION / INFORMATION ON INGREDIENTS

 

Component                                                        % Comp
CAS #    EINECS #              TLV (units)

 

Aliphatic Hydrocarbons

 

EEC LABEL SYMBOL AND CLASSIFICATION

 

R:

 

 

 

 

11-38

 

100

 

400 ppm

Highly flammable.  Irritating to skin.

S:  (2-) 16-23-24-62

Keep out of the reach of children.  Keep away from sources of ignition -
No Smoking.  Do not breathe fumes.  Avoid contact with the skin.  If
swallowed, do not induce vomiting:  Seek medical advice immediately and
show this container or label.

 

 

 

3. HAZARDS IDENTIFICATION

APPEARANCE AND ODOR:      Clear, colorless liquid

 

EMERGENCY OVERVIEW - IMMEDIATE HAZARD

HIGHLY FLAMMABLE. PRODUCT IS SLIGHTLY IRRITATING TO EYES (NO INJURY).
HIGH VAPOR MAY CAUSE RESPIRATORY TRACT IRRITATION, HEADACHE, DIZZINESS,
ANESTHESIA, DROWSINESS, UNCONSCIOUSNESS, OR DEATH.  INGESTION: MINIMAL
TOXICITY.  ASPIRATION MAY LEAD TO PULMONARY INJURY AND DEATH.

EMERGENCY OVERVIEW - CHRONIC HAZARD W ARNING

 

NO CHRONIC HAZARDS SUSPECTED.

 

POTENTIAL HEALTH EFFECTS

INHALATION

 

High vapor/aerosol concentrations (greater than approximately 1000 ppm)
are irritating to the eyes and the respiratory tract, may cause
headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other
central nervous system effects, including death.

INGESTION

 

Minimal toxicity by ingestion, though small amounts of this product
aspirated into the respiratory system durin ingestion or vomiting may
cause mild to severe pulmonary injury, possibly progressing to death.

SKIN

 

Low order or toxicity.  Frequent or prolonged contact may irritate and
cause dermatitis.  Skin contact may aggravate an existing dermatitis
condition.

EYES

 

Slightly irritating but does not injure eye tissue.

 

SIGNS AND SYMPTOMS 0F OVEREXPOSURE

INHALATION

 

Page 1
OptiClear E

Headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other
central nervous system effects, including death.

INGESTION

 

Minimal toxicity by ingestion. SKIN

Dermatitis may occur with frequent or prolonged contact. EYES

Product is only slightly irritating to eye tissue, non injurious.
CARCINOGENICITY

Not listed by NTP, IARC, or OSHA. MUTAGENICITY

No information available. REPRODUCTIVE TOXICITY

No information available. TERATOGENIC EFFECTS

No information available. ROUTES OF ENTRY

Inhalation or by skin contact. TARGET ORGAN STATEMENT

No information available.

 

4. FIRST AID MEASURES

INHALATION:

Remove to fresh air.  If not breathing, give artificial respiration.  If
breathing is difficult, give oxygen.  Call a physicia

 

 

INGESTION:

Do not induce vomiting.  If swallowed and the person is conscious,
immediately give large amounts of water.  Get medical attention.

 

SKIN:

Immediately flush skin with plenty of soap and water for at least 15
minutes while removing contaminated clothing and shoes.  Get medical
attention.  Wash clothing before reuse.  Thoroughly clean shoes before
reuse.

 

EYES:

Immediately flush eyes with plenty of water for at least fifteen
minutes, lifting lower and upper eyelids occasionally. Get medical
attention immediately.

 

5. FIRE FIGHTING MEASURES

FLASH POINT:

 

FLASH POINT METHOD:

45F (7C)

 

TCC

FLAMMABLE LIMITS:

 

AUTOIGNITION TEMPERATURE:

LEL: 1.3%; UEL: 10.2% @ 77 F

 

720 F

 

EXTINGUISHING MEDIA

Dry powder, foam, carbon dioxide. (W ater may be ineffective.)

 

 

PROTECTIVE EQUIPMENT

In the event of a fire, wear full protective clothing and NIOSH-approved
self-contained breathing apparatus with full facepiece operated in the
pressure demand or other positive pressure mode.

 

 

 

 

Page 2
OptiClear E

HAZARDOUS COMBUSTION PRODUCTS:

Thermal decomposition products may include carbon monoxide, carbon
dioxide, and hydrocarbons.

 

 

UNUSUAL FIRE AND EXPLOSION HAZARDS

Flammable liquid.  Vapor forms explosive mixtures with air.  Vapor may
travel considerable distances to ignition source and flash back.

 

NFPA CODES: Health:  1

 

Flammability: 3

 

Reactivity:   0

 

6. ACCIDENTAL RELEASE MEASURES

STEPS TO BE TAKEN IN CASE MATERIAL IS RELEASED OR SPILLED

Eliminate source of ignition.  Ventilate area.  Cover with absorbent
material (soda ash) to confine spill and sweep or shovel into container.
Close container tightly.  Avoid breathing vapors.

 

W ASTE DISPOSAL METHOD

Disposal must be made in accordance with applicable federal, state, and
local regulations.

 

 

PERSONAL PRECAUTIONS

Wear appropriate protective equipment as specified in section 8.

 

 

 

7. HANDLING AND STORAGE

HANDLING

Avoid contact and inhalation.  Do not get in eyes, on skin, on clothing.
W ash thoroughly after handling.  Transfer methods should avoid static
sparks.  Use explosion proof ventilation.

 

STORAGE

Keep in a tightly closed container, stored in a cooled, dry, ventilated
area away from sources of heat or ignition. Protect from physical
damage.  Isolate from incompatible materials (section 10).

 

STORAGE TEMPERATURE:

Room Temperature

 

DISPOSAL

Observe all national, state, and local regulations regarding product
disposal.  Containers of this material may be hazardous when empty since
they retain product residues (vapors, liquids).

 

8. EXPOSURE CONTROLS / PERSONAL PROTECTION

AIRBORNE EXPOSURE LIMITS:

Component:

Aliphatic Hydrocarbons

ACGIH Threshold Lim it Value (TLV): OSHA Permissible Exposure Lim it
(PEL):

ENGINEERING CONTROLS

400 ppm

A system of local and/or general exhaust is recommended to keep employee
exposures below the Airborn Exposure Limits.  Local exhaust ventilation
is generally preferred because it can control the emissions of the
contaminant at it source.

RESPIRATORY PROTECTION

If the exposure limit is exceeded, wear a supplied air, full-facepiece
respirator, airlined hood, or full-facepiece self- contained breathing
apparatus.

 

 

EYE PROTECTION Safety glasses.

 

 

SKIN PROTECTION

Wear protective gloves and clean body covering clothing.

 

 

 

 

 

 

 

Page 3
OptiClear E

OTHER CONTROL MEASURES

Use explosion-proof ventilation equipment.  Special care is required
given the highly flammable nature of this material.

 

9. PHYSICAL PROPERTIES

Boiling Point

 

Melting Point

 

Vapor Pressure

mm Hg

Vapor Density

Air = 1

% Volatile by Volume

244 - 284 F Less than -76

36 mmHg @ 68 F

 

3.9

 

100

Evaporation Rate

 

Solubility in W ater pH

Specific Gravity

(H20 = 1)

1.6 (n-Bu Acetate=1) Less than 0.01% @77F

N.A.

 

0.72 @ 60 F

 

10. STABILITY AND REACTIVITY

STABILITY

Stable under normal conditions of use.

 

CONDITIONS TO AVOID Heat, sources of ignition.

HAZARDOUS DECOMPOSITION PRODUCTS None

HAZARDOUS POLYMERIZATION Will not occur

 

 

INCOMPATIBLES

 

Strong oxidizing agents.

 

 

 

11. TOXICOLOGICAL INFORMATION

PRODUCT LD50 VALUES

OptiClear E

Oral Rat LD50 (m g/kg):                 Minimal toxicity by ingestion.

OptiClear E

Derm al Rabbit LD50 (mg/kg):

Minimal toxicity by skin contact.

 

COMPONENT CANCER LIST STATUS

 

Component

 

 

NTP Carcinogen

Known               Anticipated

 

 

 

IARC Category

Aliphatic Hydrocarbons

 

12. ECOLOGICAL INFORMATION

No                         No                       None

 

No specific ecological data is available for this material.  Please
refer to Section 6 for information regarding accidental releases.

13. DISPOSAL CONSIDERATIONS

Observe all national, state, and local regulations regarding product
disposal.  Containers of this material may be hazardou when empty since
they retain product residues (vapors, liquids).

 

14. TRANSPORT INFORMATION

D.O.T.

Proper Shipping Name Petroleum Products N.O.S.

 

 

Hazard Class: 3

UN Num ber: 1268

Packing Group  3

 

 

I.A.T.A.

 

 

Page 4
OptiClear E

Proper Shipping Name Petroleum Products N.O.S.

 

 

Hazard Class: 3

UN Num ber: 1268

Packing Group  3

 

 

I.M.O.

Proper Shipping Name Petroleum Products N.O.S.

 

Hazard Class: 3

UN Num ber: 1268

Packing Group  3

 

15. REGULATORY INFORMATION

UNITED STATES

TSCA Regulatory:

All intentional ingredients are listed on the TSCA Inventory.

 

SARA 311/312 Hazard Categories

Component

 

 

Fire         Pressure    Reactivity      Acute         Chronic

 

Aliphatic Hydrocarbons

EUROPE

EEC Regulatory:

 

Yes               No               No               No               No

All intentional ingredients are listed on the European EINECS Inventory.

 

EEC LABEL SYMBOL AND CLASSIFICATION

 

R:

 

 

11-38

Highly flammable.  Irritating to skin.

S:  (2-) 16-23-24-62

Keep out of the reach of children.  Keep away from sources of ignition -
No Smoking.  Do not breathe fumes.  Avoid contact with the skin.  If
swallowed, do not induce vomiting:  Seek medical advice immediately and
show this container or label.

 

 

 

16. OTHER INFORMATION

NFPA CODES: Health:  1

Flammability: 3

Reactivity:   0

 

MANUFACTURER DISCLAIMER: The information given herein is offered in good
faith as accurate, but without guarantee.  Conditions of use and
suitability of the product for particular uses are beyond our control.
All risks of use of the product are therefore assumed by the user.
Nothing is intended as a recommendation for uses which infringe valid
patents or as extending license under valid patents.  Appropriate
warnings and safe handling procedures should be provided to handlers and
users.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Page 5
OptiClear E

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From amber.mckenzie <@t> gastrodocs.net  Fri Sep 30 09:24:55 2011
From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie)
Date: Fri Sep 30 09:25:11 2011
Subject: [Histonet] RE: Histonet Digest, Vol 94,	Issue 38 Xylene safe
	disposal vs. aliphatic hydrocarbons	e.g., Slide Bright, down the drain?
In-Reply-To: 
References: 
	
Message-ID: <5A33C952BB67F4468AF1F36D739212BC01D54A@JERRY.Gia.com>

What about Alcohol & formalin from the processor?  The diluted alcohol (100, 95, and 75%) and the alcohol from the cleaning cycle?  Do you not pour that down the drain with water?  And, what about the H&E stainer...do you not pour the bluing, hematoxylin, eosin and acid alcohol down the drain?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) [E]
Sent: Friday, September 30, 2011 9:06 AM
To: Steve McClain; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

Steve,
 I totally agree. NOTHING but water should go down the drain.
Ruth
N.I.H.

-----Original Message-----
From: Steve McClain [mailto:SteveM@mcclainlab.com] 
Sent: Friday, September 30, 2011 9:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

The argument against using toxic, yet recyclable xylene in favor of a
more expensive, less efficacious xylene-substitute like Slide Bright in
larger labs is not compelling.

Aside from low volatility of Slide-Bright, what is gained in using a
more expensive substitute whose toxicities are not well-known?

The chemical composition differs, yet most MSDS warning are the same.

The advantage for low volatility can be an advantage for small hole in
the wall lab settings, like an office Mohs lab or frozen section lab in
the OR suite where ventilation may be an issue.

 

Unlike xylene where 75% recycling yield is norm , Slide-Bright can be
recycled- how well, I don't know.

I've requested specifics from B/R for a protocol and will forward that
info later.

However, if Slide-Bright is disposed like xylene it carries the same
disposal cost.

The company indicates Slide-Bright is a flammable aliphatic hydrocarbon
which laden with paraffin may be disposed of down the drain with copious
amounts of water, yet it is the lab directors job to ensure all local
state and federal guidelines are followed.

Aren't you defeating part of your purpose in working toward a safer lab
and greener environment by dumping aliphatic hydrocarbons into your
ground water?

Steve

Steve A. McClain, MD

McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000

 

Slide-Bright MSDS follows:

Revision Date: 6/1/2006

MATERIAL SAFETY DATA SHEET

Conforms to 93/112/EC and ISO 11014-1

 

1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION

PRODUCT NAME:    OptiClear E
PRODUCT NUMBER:   OE-104

CHEMICAL NAMES/ DESCRIPTION:

Aliphatic Hydrocarbon

 

MANUFACTURER: National Diagnostics, Inc.

305 Patton Drive

Atlanta, GA 30336

TELEPHONE NUMBER: (800) 526-3867 (404) 699-2121

EMERGENCY NUMBER:

CHEMTREC (800) 424-9300

2. COMPOSITION / INFORMATION ON INGREDIENTS

 

Component                                                        % Comp
CAS #    EINECS #              TLV (units)

 

Aliphatic Hydrocarbons

 

EEC LABEL SYMBOL AND CLASSIFICATION

 

R:

 

 

 

 

11-38

 

100

 

400 ppm

Highly flammable.  Irritating to skin.

S:  (2-) 16-23-24-62

Keep out of the reach of children.  Keep away from sources of ignition -
No Smoking.  Do not breathe fumes.  Avoid contact with the skin.  If
swallowed, do not induce vomiting:  Seek medical advice immediately and
show this container or label.

 

 

 

3. HAZARDS IDENTIFICATION

APPEARANCE AND ODOR:      Clear, colorless liquid

 

EMERGENCY OVERVIEW - IMMEDIATE HAZARD

HIGHLY FLAMMABLE. PRODUCT IS SLIGHTLY IRRITATING TO EYES (NO INJURY).
HIGH VAPOR MAY CAUSE RESPIRATORY TRACT IRRITATION, HEADACHE, DIZZINESS,
ANESTHESIA, DROWSINESS, UNCONSCIOUSNESS, OR DEATH.  INGESTION: MINIMAL
TOXICITY.  ASPIRATION MAY LEAD TO PULMONARY INJURY AND DEATH.

EMERGENCY OVERVIEW - CHRONIC HAZARD W ARNING

 

NO CHRONIC HAZARDS SUSPECTED.

 

POTENTIAL HEALTH EFFECTS

INHALATION

 

High vapor/aerosol concentrations (greater than approximately 1000 ppm)
are irritating to the eyes and the respiratory tract, may cause
headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other
central nervous system effects, including death.

INGESTION

 

Minimal toxicity by ingestion, though small amounts of this product
aspirated into the respiratory system durin ingestion or vomiting may
cause mild to severe pulmonary injury, possibly progressing to death.

SKIN

 

Low order or toxicity.  Frequent or prolonged contact may irritate and
cause dermatitis.  Skin contact may aggravate an existing dermatitis
condition.

EYES

 

Slightly irritating but does not injure eye tissue.

 

SIGNS AND SYMPTOMS 0F OVEREXPOSURE

INHALATION

 

Page 1
OptiClear E

Headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other
central nervous system effects, including death.

INGESTION

 

Minimal toxicity by ingestion. SKIN

Dermatitis may occur with frequent or prolonged contact. EYES

Product is only slightly irritating to eye tissue, non injurious.
CARCINOGENICITY

Not listed by NTP, IARC, or OSHA. MUTAGENICITY

No information available. REPRODUCTIVE TOXICITY

No information available. TERATOGENIC EFFECTS

No information available. ROUTES OF ENTRY

Inhalation or by skin contact. TARGET ORGAN STATEMENT

No information available.

 

4. FIRST AID MEASURES

INHALATION:

Remove to fresh air.  If not breathing, give artificial respiration.  If
breathing is difficult, give oxygen.  Call a physicia

 

 

INGESTION:

Do not induce vomiting.  If swallowed and the person is conscious,
immediately give large amounts of water.  Get medical attention.

 

SKIN:

Immediately flush skin with plenty of soap and water for at least 15
minutes while removing contaminated clothing and shoes.  Get medical
attention.  Wash clothing before reuse.  Thoroughly clean shoes before
reuse.

 

EYES:

Immediately flush eyes with plenty of water for at least fifteen
minutes, lifting lower and upper eyelids occasionally. Get medical
attention immediately.

 

5. FIRE FIGHTING MEASURES

FLASH POINT:

 

FLASH POINT METHOD:

45F (7C)

 

TCC

FLAMMABLE LIMITS:

 

AUTOIGNITION TEMPERATURE:

LEL: 1.3%; UEL: 10.2% @ 77 F

 

720 F

 

EXTINGUISHING MEDIA

Dry powder, foam, carbon dioxide. (W ater may be ineffective.)

 

 

PROTECTIVE EQUIPMENT

In the event of a fire, wear full protective clothing and NIOSH-approved
self-contained breathing apparatus with full facepiece operated in the
pressure demand or other positive pressure mode.

 

 

 

 

Page 2
OptiClear E

HAZARDOUS COMBUSTION PRODUCTS:

Thermal decomposition products may include carbon monoxide, carbon
dioxide, and hydrocarbons.

 

 

UNUSUAL FIRE AND EXPLOSION HAZARDS

Flammable liquid.  Vapor forms explosive mixtures with air.  Vapor may
travel considerable distances to ignition source and flash back.

 

NFPA CODES: Health:  1

 

Flammability: 3

 

Reactivity:   0

 

6. ACCIDENTAL RELEASE MEASURES

STEPS TO BE TAKEN IN CASE MATERIAL IS RELEASED OR SPILLED

Eliminate source of ignition.  Ventilate area.  Cover with absorbent
material (soda ash) to confine spill and sweep or shovel into container.
Close container tightly.  Avoid breathing vapors.

 

W ASTE DISPOSAL METHOD

Disposal must be made in accordance with applicable federal, state, and
local regulations.

 

 

PERSONAL PRECAUTIONS

Wear appropriate protective equipment as specified in section 8.

 

 

 

7. HANDLING AND STORAGE

HANDLING

Avoid contact and inhalation.  Do not get in eyes, on skin, on clothing.
W ash thoroughly after handling.  Transfer methods should avoid static
sparks.  Use explosion proof ventilation.

 

STORAGE

Keep in a tightly closed container, stored in a cooled, dry, ventilated
area away from sources of heat or ignition. Protect from physical
damage.  Isolate from incompatible materials (section 10).

 

STORAGE TEMPERATURE:

Room Temperature

 

DISPOSAL

Observe all national, state, and local regulations regarding product
disposal.  Containers of this material may be hazardous when empty since
they retain product residues (vapors, liquids).

 

8. EXPOSURE CONTROLS / PERSONAL PROTECTION

AIRBORNE EXPOSURE LIMITS:

Component:

Aliphatic Hydrocarbons

ACGIH Threshold Lim it Value (TLV): OSHA Permissible Exposure Lim it
(PEL):

ENGINEERING CONTROLS

400 ppm

A system of local and/or general exhaust is recommended to keep employee
exposures below the Airborn Exposure Limits.  Local exhaust ventilation
is generally preferred because it can control the emissions of the
contaminant at it source.

RESPIRATORY PROTECTION

If the exposure limit is exceeded, wear a supplied air, full-facepiece
respirator, airlined hood, or full-facepiece self- contained breathing
apparatus.

 

 

EYE PROTECTION Safety glasses.

 

 

SKIN PROTECTION

Wear protective gloves and clean body covering clothing.

 

 

 

 

 

 

 

Page 3
OptiClear E

OTHER CONTROL MEASURES

Use explosion-proof ventilation equipment.  Special care is required
given the highly flammable nature of this material.

 

9. PHYSICAL PROPERTIES

Boiling Point

 

Melting Point

 

Vapor Pressure

mm Hg

Vapor Density

Air = 1

% Volatile by Volume

244 - 284 F Less than -76

36 mmHg @ 68 F

 

3.9

 

100

Evaporation Rate

 

Solubility in W ater pH

Specific Gravity

(H20 = 1)

1.6 (n-Bu Acetate=1) Less than 0.01% @77F

N.A.

 

0.72 @ 60 F

 

10. STABILITY AND REACTIVITY

STABILITY

Stable under normal conditions of use.

 

CONDITIONS TO AVOID Heat, sources of ignition.

HAZARDOUS DECOMPOSITION PRODUCTS None

HAZARDOUS POLYMERIZATION Will not occur

 

 

INCOMPATIBLES

 

Strong oxidizing agents.

 

 

 

11. TOXICOLOGICAL INFORMATION

PRODUCT LD50 VALUES

OptiClear E

Oral Rat LD50 (m g/kg):                 Minimal toxicity by ingestion.

OptiClear E

Derm al Rabbit LD50 (mg/kg):

Minimal toxicity by skin contact.

 

COMPONENT CANCER LIST STATUS

 

Component

 

 

NTP Carcinogen

Known               Anticipated

 

 

 

IARC Category

Aliphatic Hydrocarbons

 

12. ECOLOGICAL INFORMATION

No                         No                       None

 

No specific ecological data is available for this material.  Please
refer to Section 6 for information regarding accidental releases.

13. DISPOSAL CONSIDERATIONS

Observe all national, state, and local regulations regarding product
disposal.  Containers of this material may be hazardou when empty since
they retain product residues (vapors, liquids).

 

14. TRANSPORT INFORMATION

D.O.T.

Proper Shipping Name Petroleum Products N.O.S.

 

 

Hazard Class: 3

UN Num ber: 1268

Packing Group  3

 

 

I.A.T.A.

 

 

Page 4
OptiClear E

Proper Shipping Name Petroleum Products N.O.S.

 

 

Hazard Class: 3

UN Num ber: 1268

Packing Group  3

 

 

I.M.O.

Proper Shipping Name Petroleum Products N.O.S.

 

Hazard Class: 3

UN Num ber: 1268

Packing Group  3

 

15. REGULATORY INFORMATION

UNITED STATES

TSCA Regulatory:

All intentional ingredients are listed on the TSCA Inventory.

 

SARA 311/312 Hazard Categories

Component

 

 

Fire         Pressure    Reactivity      Acute         Chronic

 

Aliphatic Hydrocarbons

EUROPE

EEC Regulatory:

 

Yes               No               No               No               No

All intentional ingredients are listed on the European EINECS Inventory.

 

EEC LABEL SYMBOL AND CLASSIFICATION

 

R:

 

 

11-38

Highly flammable.  Irritating to skin.

S:  (2-) 16-23-24-62

Keep out of the reach of children.  Keep away from sources of ignition -
No Smoking.  Do not breathe fumes.  Avoid contact with the skin.  If
swallowed, do not induce vomiting:  Seek medical advice immediately and
show this container or label.

 

 

 

16. OTHER INFORMATION

NFPA CODES: Health:  1

Flammability: 3

Reactivity:   0

 

MANUFACTURER DISCLAIMER: The information given herein is offered in good
faith as accurate, but without guarantee.  Conditions of use and
suitability of the product for particular uses are beyond our control.
All risks of use of the product are therefore assumed by the user.
Nothing is intended as a recommendation for uses which infringe valid
patents or as extending license under valid patents.  Appropriate
warnings and safe handling procedures should be provided to handlers and
users.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Page 5
OptiClear E

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From joseph-galbraith <@t> uiowa.edu  Fri Sep 30 09:34:50 2011
From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe)
Date: Fri Sep 30 09:34:57 2011
Subject: [Histonet] RE: Histonet Digest, Vol 94,	Issue 38 Xylene safe
	disposal vs. aliphatic hydrocarbons	e.g., Slide Bright, down the drain?
In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC01D54A@JERRY.Gia.com>
References: 
	
	<5A33C952BB67F4468AF1F36D739212BC01D54A@JERRY.Gia.com>
Message-ID: <6DC87DEA9229894DB3A09F8B61717A46106F5280@hc-mailboxc1-n3.healthcare.uiowa.edu>

Amber:  Actually, no we do not dump the items you listed down the drain.  They get recycled appropriately.  Joe

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Friday, September 30, 2011 9:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

What about Alcohol & formalin from the processor?  The diluted alcohol (100, 95, and 75%) and the alcohol from the cleaning cycle?  Do you not pour that down the drain with water?  And, what about the H&E stainer...do you not pour the bluing, hematoxylin, eosin and acid alcohol down the drain?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) [E]
Sent: Friday, September 30, 2011 9:06 AM
To: Steve McClain; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

Steve,
 I totally agree. NOTHING but water should go down the drain.
Ruth
N.I.H.

-----Original Message-----
From: Steve McClain [mailto:SteveM@mcclainlab.com]
Sent: Friday, September 30, 2011 9:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

The argument against using toxic, yet recyclable xylene in favor of a
more expensive, less efficacious xylene-substitute like Slide Bright in
larger labs is not compelling.

Aside from low volatility of Slide-Bright, what is gained in using a
more expensive substitute whose toxicities are not well-known?

The chemical composition differs, yet most MSDS warning are the same.

The advantage for low volatility can be an advantage for small hole in
the wall lab settings, like an office Mohs lab or frozen section lab in
the OR suite where ventilation may be an issue.



Unlike xylene where 75% recycling yield is norm , Slide-Bright can be
recycled- how well, I don't know.

I've requested specifics from B/R for a protocol and will forward that
info later.

However, if Slide-Bright is disposed like xylene it carries the same
disposal cost.

The company indicates Slide-Bright is a flammable aliphatic hydrocarbon
which laden with paraffin may be disposed of down the drain with copious
amounts of water, yet it is the lab directors job to ensure all local
state and federal guidelines are followed.

Aren't you defeating part of your purpose in working toward a safer lab
and greener environment by dumping aliphatic hydrocarbons into your
ground water?

Steve

Steve A. McClain, MD

McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000



Slide-Bright MSDS follows:

Revision Date: 6/1/2006

MATERIAL SAFETY DATA SHEET

Conforms to 93/112/EC and ISO 11014-1



1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION

PRODUCT NAME:    OptiClear E
PRODUCT NUMBER:   OE-104

CHEMICAL NAMES/ DESCRIPTION:

Aliphatic Hydrocarbon



MANUFACTURER: National Diagnostics, Inc.

305 Patton Drive

Atlanta, GA 30336

TELEPHONE NUMBER: (800) 526-3867 (404) 699-2121

EMERGENCY NUMBER:

CHEMTREC (800) 424-9300

2. COMPOSITION / INFORMATION ON INGREDIENTS



Component                                                        % Comp
CAS #    EINECS #              TLV (units)



Aliphatic Hydrocarbons



EEC LABEL SYMBOL AND CLASSIFICATION



R:









11-38



100



400 ppm

Highly flammable.  Irritating to skin.

S:  (2-) 16-23-24-62

Keep out of the reach of children.  Keep away from sources of ignition -
No Smoking.  Do not breathe fumes.  Avoid contact with the skin.  If
swallowed, do not induce vomiting:  Seek medical advice immediately and
show this container or label.







3. HAZARDS IDENTIFICATION

APPEARANCE AND ODOR:      Clear, colorless liquid



EMERGENCY OVERVIEW - IMMEDIATE HAZARD

HIGHLY FLAMMABLE. PRODUCT IS SLIGHTLY IRRITATING TO EYES (NO INJURY).
HIGH VAPOR MAY CAUSE RESPIRATORY TRACT IRRITATION, HEADACHE, DIZZINESS,
ANESTHESIA, DROWSINESS, UNCONSCIOUSNESS, OR DEATH.  INGESTION: MINIMAL
TOXICITY.  ASPIRATION MAY LEAD TO PULMONARY INJURY AND DEATH.

EMERGENCY OVERVIEW - CHRONIC HAZARD W ARNING



NO CHRONIC HAZARDS SUSPECTED.



POTENTIAL HEALTH EFFECTS

INHALATION



High vapor/aerosol concentrations (greater than approximately 1000 ppm)
are irritating to the eyes and the respiratory tract, may cause
headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other
central nervous system effects, including death.

INGESTION



Minimal toxicity by ingestion, though small amounts of this product
aspirated into the respiratory system durin ingestion or vomiting may
cause mild to severe pulmonary injury, possibly progressing to death.

SKIN



Low order or toxicity.  Frequent or prolonged contact may irritate and
cause dermatitis.  Skin contact may aggravate an existing dermatitis
condition.

EYES



Slightly irritating but does not injure eye tissue.



SIGNS AND SYMPTOMS 0F OVEREXPOSURE

INHALATION



Page 1
OptiClear E

Headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other
central nervous system effects, including death.

INGESTION



Minimal toxicity by ingestion. SKIN

Dermatitis may occur with frequent or prolonged contact. EYES

Product is only slightly irritating to eye tissue, non injurious.
CARCINOGENICITY

Not listed by NTP, IARC, or OSHA. MUTAGENICITY

No information available. REPRODUCTIVE TOXICITY

No information available. TERATOGENIC EFFECTS

No information available. ROUTES OF ENTRY

Inhalation or by skin contact. TARGET ORGAN STATEMENT

No information available.



4. FIRST AID MEASURES

INHALATION:

Remove to fresh air.  If not breathing, give artificial respiration.  If
breathing is difficult, give oxygen.  Call a physicia





INGESTION:

Do not induce vomiting.  If swallowed and the person is conscious,
immediately give large amounts of water.  Get medical attention.



SKIN:

Immediately flush skin with plenty of soap and water for at least 15
minutes while removing contaminated clothing and shoes.  Get medical
attention.  Wash clothing before reuse.  Thoroughly clean shoes before
reuse.



EYES:

Immediately flush eyes with plenty of water for at least fifteen
minutes, lifting lower and upper eyelids occasionally. Get medical
attention immediately.



5. FIRE FIGHTING MEASURES

FLASH POINT:



FLASH POINT METHOD:

45F (7C)



TCC

FLAMMABLE LIMITS:



AUTOIGNITION TEMPERATURE:

LEL: 1.3%; UEL: 10.2% @ 77 F



720 F



EXTINGUISHING MEDIA

Dry powder, foam, carbon dioxide. (W ater may be ineffective.)





PROTECTIVE EQUIPMENT

In the event of a fire, wear full protective clothing and NIOSH-approved
self-contained breathing apparatus with full facepiece operated in the
pressure demand or other positive pressure mode.









Page 2
OptiClear E

HAZARDOUS COMBUSTION PRODUCTS:

Thermal decomposition products may include carbon monoxide, carbon
dioxide, and hydrocarbons.





UNUSUAL FIRE AND EXPLOSION HAZARDS

Flammable liquid.  Vapor forms explosive mixtures with air.  Vapor may
travel considerable distances to ignition source and flash back.



NFPA CODES: Health:  1



Flammability: 3



Reactivity:   0



6. ACCIDENTAL RELEASE MEASURES

STEPS TO BE TAKEN IN CASE MATERIAL IS RELEASED OR SPILLED

Eliminate source of ignition.  Ventilate area.  Cover with absorbent
material (soda ash) to confine spill and sweep or shovel into container.
Close container tightly.  Avoid breathing vapors.



W ASTE DISPOSAL METHOD

Disposal must be made in accordance with applicable federal, state, and
local regulations.





PERSONAL PRECAUTIONS

Wear appropriate protective equipment as specified in section 8.







7. HANDLING AND STORAGE

HANDLING

Avoid contact and inhalation.  Do not get in eyes, on skin, on clothing.
W ash thoroughly after handling.  Transfer methods should avoid static
sparks.  Use explosion proof ventilation.



STORAGE

Keep in a tightly closed container, stored in a cooled, dry, ventilated
area away from sources of heat or ignition. Protect from physical
damage.  Isolate from incompatible materials (section 10).



STORAGE TEMPERATURE:

Room Temperature



DISPOSAL

Observe all national, state, and local regulations regarding product
disposal.  Containers of this material may be hazardous when empty since
they retain product residues (vapors, liquids).



8. EXPOSURE CONTROLS / PERSONAL PROTECTION

AIRBORNE EXPOSURE LIMITS:

Component:

Aliphatic Hydrocarbons

ACGIH Threshold Lim it Value (TLV): OSHA Permissible Exposure Lim it
(PEL):

ENGINEERING CONTROLS

400 ppm

A system of local and/or general exhaust is recommended to keep employee
exposures below the Airborn Exposure Limits.  Local exhaust ventilation
is generally preferred because it can control the emissions of the
contaminant at it source.

RESPIRATORY PROTECTION

If the exposure limit is exceeded, wear a supplied air, full-facepiece
respirator, airlined hood, or full-facepiece self- contained breathing
apparatus.





EYE PROTECTION Safety glasses.





SKIN PROTECTION

Wear protective gloves and clean body covering clothing.















Page 3
OptiClear E

OTHER CONTROL MEASURES

Use explosion-proof ventilation equipment.  Special care is required
given the highly flammable nature of this material.



9. PHYSICAL PROPERTIES

Boiling Point



Melting Point



Vapor Pressure

mm Hg

Vapor Density

Air = 1

% Volatile by Volume

244 - 284 F Less than -76

36 mmHg @ 68 F



3.9



100

Evaporation Rate



Solubility in W ater pH

Specific Gravity

(H20 = 1)

1.6 (n-Bu Acetate=1) Less than 0.01% @77F

N.A.



0.72 @ 60 F



10. STABILITY AND REACTIVITY

STABILITY

Stable under normal conditions of use.



CONDITIONS TO AVOID Heat, sources of ignition.

HAZARDOUS DECOMPOSITION PRODUCTS None

HAZARDOUS POLYMERIZATION Will not occur





INCOMPATIBLES



Strong oxidizing agents.







11. TOXICOLOGICAL INFORMATION

PRODUCT LD50 VALUES

OptiClear E

Oral Rat LD50 (m g/kg):                 Minimal toxicity by ingestion.

OptiClear E

Derm al Rabbit LD50 (mg/kg):

Minimal toxicity by skin contact.



COMPONENT CANCER LIST STATUS



Component





NTP Carcinogen

Known               Anticipated







IARC Category

Aliphatic Hydrocarbons



12. ECOLOGICAL INFORMATION

No                         No                       None



No specific ecological data is available for this material.  Please
refer to Section 6 for information regarding accidental releases.

13. DISPOSAL CONSIDERATIONS

Observe all national, state, and local regulations regarding product
disposal.  Containers of this material may be hazardou when empty since
they retain product residues (vapors, liquids).



14. TRANSPORT INFORMATION

D.O.T.

Proper Shipping Name Petroleum Products N.O.S.





Hazard Class: 3

UN Num ber: 1268

Packing Group  3





I.A.T.A.





Page 4
OptiClear E

Proper Shipping Name Petroleum Products N.O.S.





Hazard Class: 3

UN Num ber: 1268

Packing Group  3





I.M.O.

Proper Shipping Name Petroleum Products N.O.S.



Hazard Class: 3

UN Num ber: 1268

Packing Group  3



15. REGULATORY INFORMATION

UNITED STATES

TSCA Regulatory:

All intentional ingredients are listed on the TSCA Inventory.



SARA 311/312 Hazard Categories

Component





Fire         Pressure    Reactivity      Acute         Chronic



Aliphatic Hydrocarbons

EUROPE

EEC Regulatory:



Yes               No               No               No               No

All intentional ingredients are listed on the European EINECS Inventory.



EEC LABEL SYMBOL AND CLASSIFICATION



R:





11-38

Highly flammable.  Irritating to skin.

S:  (2-) 16-23-24-62

Keep out of the reach of children.  Keep away from sources of ignition -
No Smoking.  Do not breathe fumes.  Avoid contact with the skin.  If
swallowed, do not induce vomiting:  Seek medical advice immediately and
show this container or label.







16. OTHER INFORMATION

NFPA CODES: Health:  1

Flammability: 3

Reactivity:   0



MANUFACTURER DISCLAIMER: The information given herein is offered in good
faith as accurate, but without guarantee.  Conditions of use and
suitability of the product for particular uses are beyond our control.
All risks of use of the product are therefore assumed by the user.
Nothing is intended as a recommendation for uses which infringe valid
patents or as extending license under valid patents.  Appropriate
warnings and safe handling procedures should be provided to handlers and
users.





































Page 5
OptiClear E



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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


________________________________
Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.  If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.  Please reply to the sender that you have received the message in error, then delete it.  Thank you.
________________________________

From jsjurczak <@t> comcast.net  Fri Sep 30 09:43:04 2011
From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net)
Date: Fri Sep 30 09:43:16 2011
Subject: [Histonet] Alignment tool
Message-ID: <628451078.1371101.1317393784705.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net>

Who sells the microtome alignment tool that clamps onto the base of the microtome and also into the cassette holder to assure that all microtomes in the lab are in the same cutting plane? Do the bubble ones work as well? 
From jqb7 <@t> cdc.gov  Fri Sep 30 09:44:30 2011
From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID))
Date: Fri Sep 30 09:44:34 2011
Subject: [Histonet] Alignment tool
In-Reply-To: <628451078.1371101.1317393784705.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net>
References: <628451078.1371101.1317393784705.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net>
Message-ID: 

Newcomer sells one

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jsjurczak@comcast.net
Sent: Friday, September 30, 2011 10:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Alignment tool

Who sells the microtome alignment tool that clamps onto the base of the microtome and also into the cassette holder to assure that all microtomes in the lab are in the same cutting plane? Do the bubble ones work as well? 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From brett_connolly <@t> merck.com  Fri Sep 30 09:47:13 2011
From: brett_connolly <@t> merck.com (Connolly, Brett M)
Date: Fri Sep 30 09:47:20 2011
Subject: [Histonet] RE: Histonet Digest, Vol 94,	Issue 38 Xylene safe
	disposal vs. aliphatic hydrocarbons	e.g., Slide Bright, down the drain?
In-Reply-To: <6DC87DEA9229894DB3A09F8B61717A46106F5280@hc-mailboxc1-n3.healthcare.uiowa.edu>
References: 
	
	<5A33C952BB67F4468AF1F36D739212BC01D54A@JERRY.Gia.com>
	<6DC87DEA9229894DB3A09F8B61717A46106F5280@hc-mailboxc1-n3.healthcare.uiowa.edu>
Message-ID: 

We collect EVERYTHING for recycling or disposal by our site-wide contractor http://www.cleanharbors.com/

Only environmentally approved hand soap and glassware cleansers are allowed down the drain, along with water.

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly@merck.com
T- 215-652-2501
F- 215-993-6803



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe
Sent: Friday, September 30, 2011 10:35 AM
To: Amber McKenzie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

Amber:  Actually, no we do not dump the items you listed down the drain.  They get recycled appropriately.  Joe

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Friday, September 30, 2011 9:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

What about Alcohol & formalin from the processor?  The diluted alcohol (100, 95, and 75%) and the alcohol from the cleaning cycle?  Do you not pour that down the drain with water?  And, what about the H&E stainer...do you not pour the bluing, hematoxylin, eosin and acid alcohol down the drain?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) [E]
Sent: Friday, September 30, 2011 9:06 AM
To: Steve McClain; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

Steve,
 I totally agree. NOTHING but water should go down the drain.
Ruth
N.I.H.

-----Original Message-----
From: Steve McClain [mailto:SteveM@mcclainlab.com]
Sent: Friday, September 30, 2011 9:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

The argument against using toxic, yet recyclable xylene in favor of a
more expensive, less efficacious xylene-substitute like Slide Bright in
larger labs is not compelling.

Aside from low volatility of Slide-Bright, what is gained in using a
more expensive substitute whose toxicities are not well-known?

The chemical composition differs, yet most MSDS warning are the same.

The advantage for low volatility can be an advantage for small hole in
the wall lab settings, like an office Mohs lab or frozen section lab in
the OR suite where ventilation may be an issue.



Unlike xylene where 75% recycling yield is norm , Slide-Bright can be
recycled- how well, I don't know.

I've requested specifics from B/R for a protocol and will forward that
info later.

However, if Slide-Bright is disposed like xylene it carries the same
disposal cost.

The company indicates Slide-Bright is a flammable aliphatic hydrocarbon
which laden with paraffin may be disposed of down the drain with copious
amounts of water, yet it is the lab directors job to ensure all local
state and federal guidelines are followed.

Aren't you defeating part of your purpose in working toward a safer lab
and greener environment by dumping aliphatic hydrocarbons into your
ground water?

Steve

Steve A. McClain, MD

McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000



Slide-Bright MSDS follows:

Revision Date: 6/1/2006

MATERIAL SAFETY DATA SHEET

Conforms to 93/112/EC and ISO 11014-1



1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION

PRODUCT NAME:    OptiClear E
PRODUCT NUMBER:   OE-104

CHEMICAL NAMES/ DESCRIPTION:

Aliphatic Hydrocarbon



MANUFACTURER: National Diagnostics, Inc.

305 Patton Drive

Atlanta, GA 30336

TELEPHONE NUMBER: (800) 526-3867 (404) 699-2121

EMERGENCY NUMBER:

CHEMTREC (800) 424-9300

2. COMPOSITION / INFORMATION ON INGREDIENTS



Component                                                        % Comp
CAS #    EINECS #              TLV (units)



Aliphatic Hydrocarbons



EEC LABEL SYMBOL AND CLASSIFICATION



R:









11-38



100



400 ppm

Highly flammable.  Irritating to skin.

S:  (2-) 16-23-24-62

Keep out of the reach of children.  Keep away from sources of ignition -
No Smoking.  Do not breathe fumes.  Avoid contact with the skin.  If
swallowed, do not induce vomiting:  Seek medical advice immediately and
show this container or label.







3. HAZARDS IDENTIFICATION

APPEARANCE AND ODOR:      Clear, colorless liquid



EMERGENCY OVERVIEW - IMMEDIATE HAZARD

HIGHLY FLAMMABLE. PRODUCT IS SLIGHTLY IRRITATING TO EYES (NO INJURY).
HIGH VAPOR MAY CAUSE RESPIRATORY TRACT IRRITATION, HEADACHE, DIZZINESS,
ANESTHESIA, DROWSINESS, UNCONSCIOUSNESS, OR DEATH.  INGESTION: MINIMAL
TOXICITY.  ASPIRATION MAY LEAD TO PULMONARY INJURY AND DEATH.

EMERGENCY OVERVIEW - CHRONIC HAZARD W ARNING



NO CHRONIC HAZARDS SUSPECTED.



POTENTIAL HEALTH EFFECTS

INHALATION



High vapor/aerosol concentrations (greater than approximately 1000 ppm)
are irritating to the eyes and the respiratory tract, may cause
headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other
central nervous system effects, including death.

INGESTION



Minimal toxicity by ingestion, though small amounts of this product
aspirated into the respiratory system durin ingestion or vomiting may
cause mild to severe pulmonary injury, possibly progressing to death.

SKIN



Low order or toxicity.  Frequent or prolonged contact may irritate and
cause dermatitis.  Skin contact may aggravate an existing dermatitis
condition.

EYES



Slightly irritating but does not injure eye tissue.



SIGNS AND SYMPTOMS 0F OVEREXPOSURE

INHALATION



Page 1
OptiClear E

Headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other
central nervous system effects, including death.

INGESTION



Minimal toxicity by ingestion. SKIN

Dermatitis may occur with frequent or prolonged contact. EYES

Product is only slightly irritating to eye tissue, non injurious.
CARCINOGENICITY

Not listed by NTP, IARC, or OSHA. MUTAGENICITY

No information available. REPRODUCTIVE TOXICITY

No information available. TERATOGENIC EFFECTS

No information available. ROUTES OF ENTRY

Inhalation or by skin contact. TARGET ORGAN STATEMENT

No information available.



4. FIRST AID MEASURES

INHALATION:

Remove to fresh air.  If not breathing, give artificial respiration.  If
breathing is difficult, give oxygen.  Call a physicia





INGESTION:

Do not induce vomiting.  If swallowed and the person is conscious,
immediately give large amounts of water.  Get medical attention.



SKIN:

Immediately flush skin with plenty of soap and water for at least 15
minutes while removing contaminated clothing and shoes.  Get medical
attention.  Wash clothing before reuse.  Thoroughly clean shoes before
reuse.



EYES:

Immediately flush eyes with plenty of water for at least fifteen
minutes, lifting lower and upper eyelids occasionally. Get medical
attention immediately.



5. FIRE FIGHTING MEASURES

FLASH POINT:



FLASH POINT METHOD:

45F (7C)



TCC

FLAMMABLE LIMITS:



AUTOIGNITION TEMPERATURE:

LEL: 1.3%; UEL: 10.2% @ 77 F



720 F



EXTINGUISHING MEDIA

Dry powder, foam, carbon dioxide. (W ater may be ineffective.)





PROTECTIVE EQUIPMENT

In the event of a fire, wear full protective clothing and NIOSH-approved
self-contained breathing apparatus with full facepiece operated in the
pressure demand or other positive pressure mode.









Page 2
OptiClear E

HAZARDOUS COMBUSTION PRODUCTS:

Thermal decomposition products may include carbon monoxide, carbon
dioxide, and hydrocarbons.





UNUSUAL FIRE AND EXPLOSION HAZARDS

Flammable liquid.  Vapor forms explosive mixtures with air.  Vapor may
travel considerable distances to ignition source and flash back.



NFPA CODES: Health:  1



Flammability: 3



Reactivity:   0



6. ACCIDENTAL RELEASE MEASURES

STEPS TO BE TAKEN IN CASE MATERIAL IS RELEASED OR SPILLED

Eliminate source of ignition.  Ventilate area.  Cover with absorbent
material (soda ash) to confine spill and sweep or shovel into container.
Close container tightly.  Avoid breathing vapors.



W ASTE DISPOSAL METHOD

Disposal must be made in accordance with applicable federal, state, and
local regulations.





PERSONAL PRECAUTIONS

Wear appropriate protective equipment as specified in section 8.







7. HANDLING AND STORAGE

HANDLING

Avoid contact and inhalation.  Do not get in eyes, on skin, on clothing.
W ash thoroughly after handling.  Transfer methods should avoid static
sparks.  Use explosion proof ventilation.



STORAGE

Keep in a tightly closed container, stored in a cooled, dry, ventilated
area away from sources of heat or ignition. Protect from physical
damage.  Isolate from incompatible materials (section 10).



STORAGE TEMPERATURE:

Room Temperature



DISPOSAL

Observe all national, state, and local regulations regarding product
disposal.  Containers of this material may be hazardous when empty since
they retain product residues (vapors, liquids).



8. EXPOSURE CONTROLS / PERSONAL PROTECTION

AIRBORNE EXPOSURE LIMITS:

Component:

Aliphatic Hydrocarbons

ACGIH Threshold Lim it Value (TLV): OSHA Permissible Exposure Lim it
(PEL):

ENGINEERING CONTROLS

400 ppm

A system of local and/or general exhaust is recommended to keep employee
exposures below the Airborn Exposure Limits.  Local exhaust ventilation
is generally preferred because it can control the emissions of the
contaminant at it source.

RESPIRATORY PROTECTION

If the exposure limit is exceeded, wear a supplied air, full-facepiece
respirator, airlined hood, or full-facepiece self- contained breathing
apparatus.





EYE PROTECTION Safety glasses.





SKIN PROTECTION

Wear protective gloves and clean body covering clothing.















Page 3
OptiClear E

OTHER CONTROL MEASURES

Use explosion-proof ventilation equipment.  Special care is required
given the highly flammable nature of this material.



9. PHYSICAL PROPERTIES

Boiling Point



Melting Point



Vapor Pressure

mm Hg

Vapor Density

Air = 1

% Volatile by Volume

244 - 284 F Less than -76

36 mmHg @ 68 F



3.9



100

Evaporation Rate



Solubility in W ater pH

Specific Gravity

(H20 = 1)

1.6 (n-Bu Acetate=1) Less than 0.01% @77F

N.A.



0.72 @ 60 F



10. STABILITY AND REACTIVITY

STABILITY

Stable under normal conditions of use.



CONDITIONS TO AVOID Heat, sources of ignition.

HAZARDOUS DECOMPOSITION PRODUCTS None

HAZARDOUS POLYMERIZATION Will not occur





INCOMPATIBLES



Strong oxidizing agents.







11. TOXICOLOGICAL INFORMATION

PRODUCT LD50 VALUES

OptiClear E

Oral Rat LD50 (m g/kg):                 Minimal toxicity by ingestion.

OptiClear E

Derm al Rabbit LD50 (mg/kg):

Minimal toxicity by skin contact.



COMPONENT CANCER LIST STATUS



Component





NTP Carcinogen

Known               Anticipated







IARC Category

Aliphatic Hydrocarbons



12. ECOLOGICAL INFORMATION

No                         No                       None



No specific ecological data is available for this material.  Please
refer to Section 6 for information regarding accidental releases.

13. DISPOSAL CONSIDERATIONS

Observe all national, state, and local regulations regarding product
disposal.  Containers of this material may be hazardou when empty since
they retain product residues (vapors, liquids).



14. TRANSPORT INFORMATION

D.O.T.

Proper Shipping Name Petroleum Products N.O.S.





Hazard Class: 3

UN Num ber: 1268

Packing Group  3





I.A.T.A.





Page 4
OptiClear E

Proper Shipping Name Petroleum Products N.O.S.





Hazard Class: 3

UN Num ber: 1268

Packing Group  3





I.M.O.

Proper Shipping Name Petroleum Products N.O.S.



Hazard Class: 3

UN Num ber: 1268

Packing Group  3



15. REGULATORY INFORMATION

UNITED STATES

TSCA Regulatory:

All intentional ingredients are listed on the TSCA Inventory.



SARA 311/312 Hazard Categories

Component





Fire         Pressure    Reactivity      Acute         Chronic



Aliphatic Hydrocarbons

EUROPE

EEC Regulatory:



Yes               No               No               No               No

All intentional ingredients are listed on the European EINECS Inventory.



EEC LABEL SYMBOL AND CLASSIFICATION



R:





11-38

Highly flammable.  Irritating to skin.

S:  (2-) 16-23-24-62

Keep out of the reach of children.  Keep away from sources of ignition -
No Smoking.  Do not breathe fumes.  Avoid contact with the skin.  If
swallowed, do not induce vomiting:  Seek medical advice immediately and
show this container or label.







16. OTHER INFORMATION

NFPA CODES: Health:  1

Flammability: 3

Reactivity:   0



MANUFACTURER DISCLAIMER: The information given herein is offered in good
faith as accurate, but without guarantee.  Conditions of use and
suitability of the product for particular uses are beyond our control.
All risks of use of the product are therefore assumed by the user.
Nothing is intended as a recommendation for uses which infringe valid
patents or as extending license under valid patents.  Appropriate
warnings and safe handling procedures should be provided to handlers and
users.





































Page 5
OptiClear E



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


________________________________
Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.  If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.  Please reply to the sender that you have received the message in error, then delete it.  Thank you.
________________________________

_______________________________________________
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Histonet@lists.utsouthwestern.edu
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Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


From adam <@t> sensorhealth.com  Fri Sep 30 09:55:06 2011
From: adam <@t> sensorhealth.com (Adam Harris)
Date: Fri Sep 30 09:55:14 2011
Subject: [Histonet] RE: KP Markers!
Message-ID: 

If you would like to obtain KP Markers, they are now being carried by Sensor
Health, inc. in Cambridge, Ontario, Canada. If you have any questions you
can contact Adam Harris at 888-777-7080 or 519-241-2194. You can check out
their website at www.sensorhealth.com 



From JMyers1 <@t> aol.com  Fri Sep 30 10:02:49 2011
From: JMyers1 <@t> aol.com (JMyers1@aol.com)
Date: Fri Sep 30 10:03:02 2011
Subject: [Histonet] Background in FFPE glandular tissue sections 
Message-ID: <2eab3.2e948b9e.3bb73419@aol.com>

Mesruh:
The easiest method to address such is a problem is to apply a serum-free  
(e.g. casein-based) protein blocking agent, which can be obtained from a  
variety of vendors. This technique is particularly effective when the  reagent 
is allowed to incubate with the specimen for 5 to 10 minutes at RT, and  
then, rather than rinsing it off the slide with buffer, it is drained  off or 
blown off; that way, when the primary antibody solution is applied  
(immediately after this removal step), the primary has to 'work through'  the 
residual blocking agent, resulting in the binding of only  high-avidity/affinity 
antibodies to the target antigen. 
Good Luck,
Joe Myers, M.S., CT(ASCP)QIHC
 
------------------------------

Message: 3
Date: Thu, 29 Sep 2011  13:10:58 -0400
From: mesruh turkekul 
Subject:  [Histonet] Background in FFPE glandular tissue sections
during  IHC
To: _histonet@lists.utsouthwestern.edu_ 
(mailto:histonet@lists.utsouthwestern.edu) 

Dear Histonetters,

When I do IHC on FFPE tissue sections. Most of  time there is background due
to trapping of IgG/detection reagents probably  in the connective tissue
fibers, mucins or other secretions of the glandular  tissue. Especially
prostate and mammary gland. Any tips to get rid of that  kind of background?
Do you know any treatment  to block mucins or  connective tissue fibers from
nonspecifically and electrostatically  attracting IgGs and detection
reagents?

Regards,
Mesruh  Turkekul
mskcc.org
From sdysart <@t> mirnarx.com  Fri Sep 30 10:07:05 2011
From: sdysart <@t> mirnarx.com (Sarah Dysart)
Date: Fri Sep 30 10:07:15 2011
Subject: [Histonet] Alignment tool
In-Reply-To: <628451078.1371101.1317393784705.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net>
References: <628451078.1371101.1317393784705.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net>
Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D507755@SN2PRD0702MB110.namprd07.prod.outlook.com>

I actually just got a flyer for one of those from American Mastertech that looked pretty neat?

http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=EQS05


Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jsjurczak@comcast.net
Sent: Friday, September 30, 2011 9:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Alignment tool

Who sells the microtome alignment tool that clamps onto the base of the microtome and also into the cassette holder to assure that all microtomes in the lab are in the same cutting plane? Do the bubble ones work as well? 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From b-frederick <@t> northwestern.edu  Fri Sep 30 10:15:41 2011
From: b-frederick <@t> northwestern.edu (Bernice Frederick)
Date: Fri Sep 30 10:16:19 2011
Subject: [Histonet] Alignment tool
In-Reply-To: 
References: <628451078.1371101.1317393784705.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net>
	
Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E179FB1@evcspmbx3.ads.northwestern.edu>

So does TechOne biomedical

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick@northwestern.edu


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID)
Sent: Friday, September 30, 2011 9:45 AM
To: jsjurczak@comcast.net; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Alignment tool

Newcomer sells one

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jsjurczak@comcast.net
Sent: Friday, September 30, 2011 10:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Alignment tool

Who sells the microtome alignment tool that clamps onto the base of the microtome and also into the cassette holder to assure that all microtomes in the lab are in the same cutting plane? Do the bubble ones work as well? 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From tnsbull <@t> gmail.com  Fri Sep 30 12:29:43 2011
From: tnsbull <@t> gmail.com (Terry&Star Bullard)
Date: Fri Sep 30 12:29:47 2011
Subject: [Histonet] Control Tissue
Message-ID: 

Hi all. Does anyone have a resource for control blocks? We are in need
of fungus controls and are trying to save the cost of buying slides.
Thanks all.

Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc


Sheila,
National Society for Histotechnology has a Tissue Control Bank, If you
contact the person below she can help:

Melinda A Hamilton HT (ASCP)
NSH Tissue Control Bank Curator
Melinda Sokol 

Hope that help!
Star Bullard HT(ASCP)
From jblaine <@t> astrixinc.com  Fri Sep 30 12:33:27 2011
From: jblaine <@t> astrixinc.com (Jason Blaine)
Date: Fri Sep 30 12:33:31 2011
Subject: [Histonet] Position Available @ NIH (Bethesda, MD) 
Message-ID: 

Seeking highly skilled scientist or histologist who is well versed in IHC.  This position is in the National Institute of Mental Health (NIMH) and thus requires prior neuroscience and non-human primate experience.  Please note that we are NOT necessarily seeking ASCP histology certificated individuals and that prior related experience will be preferred over certification.

The position will support research in the NIMH.  The position will provide histology support service for the complete laboratory (has 5 sections within it).  This person will be the go to person for histology and IHC within the lab and its 5 sections.  This individual will be expected to function as a resource to all of the lab's not solely as a technician but rather as a histology/IHC expert to advise the teams with their histology/IHC needs.  This will be a collaborative research effort and will not offer personal independent research.  Non-human primate model is the principal concern.  Experience with IHC, Fluoro-ruby and green, D2, BrDu, tyrosine hyroxylase, perfusion, brain removal and prep, and much more is desired.

If this opportunity is not right for you I ask that you point me in the direction of someone who may be appropriately qualified - maybe a colleague of yours, or former boss, or collaborator.  The opportunity is a fulltime, salaried, indefinite, contractor position with benefits.

Qualified candidates please submit a current CV/resume (in MS Word format) to jblaine@astrixinc.com.

Thanks -
Jason Blaine
jblaine@astrixinc.com

From SteveM <@t> mcclainlab.com  Fri Sep 30 13:08:27 2011
From: SteveM <@t> mcclainlab.com (Steve McClain)
Date: Fri Sep 30 12:55:36 2011
Subject: [Histonet] Histonet Digest, Vol 94, Issue 40 Nothing down the drain
Message-ID: 

Yes nothing down the drain (except maybe the lithium carbonate bluing).

Hematoxylin goes into medical waste service.

Used Eosin gets reused a 10-20 ml at a time into the first absolute on
the processors as a marker of carryover (as the later alcohols turn red,
we have a visual indicator of the need to change, also helps with
embedding since collagen stains more than epithelium/epidermis).

Alcohols after the de-waxing xylenes and from the processor purges go to
the medical waste service.

Other alcohols get recycled.

All the Xylenes get recycled.

Liquid Waste from the recycler goes to medical waste service.

Paraffins go into the trash.

 

 

Steve

Steve A. McClain, MD

McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000

 

From an.eerdekens <@t> uzleuven.be  Fri Sep 30 14:18:10 2011
From: an.eerdekens <@t> uzleuven.be (An Eerdekens)
Date: Fri Sep 30 14:18:19 2011
Subject: [Histonet] washing the placenta
Message-ID: <09F2BA9A1E06C64D9F090FF0FD0D73179763AFDBC7@EX2007-MBX-2.uz.kuleuven.ac.be>

Dear all,

Is there someone with experience in washing of placentae before sampling?

Regards

Dr An Eerdekens
From mfisher <@t> ecrmc.org  Fri Sep 30 14:33:59 2011
From: mfisher <@t> ecrmc.org (Marcia Fisher)
Date: Fri Sep 30 14:34:07 2011
Subject: [Histonet] Microtome Alignment Tool
Message-ID: 

We just purchased one from American Master Tech and really like the results.  We now have added this task to our monthly maintenance sheets.

Marcia Fisher
Histology Supervisor/Lab Safety Officer
El Centro Regional Medical Center


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