[Histonet] Problems with Frozen tissues

[Igor Deyneko]

I'm looking for some advice on frozen tissues. This is the first time I'm
doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut
well onto special Gold Plus slides from Fisher. Then, when I was ready to
stain the slides, i air dried them fro an hour and wanted to do H&E and
Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on
preventing this mischief?
Thank you in advance.
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA