[Histonet] How to save dried antibody?

[Jan Shivers]

I personally would not use it; you don't know how much loss of immunologic 
reactivity has occurred, and that might affect your project data.  Buy a 
fresh supply, if possible.

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu

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----- Original Message ----- 
From: "sarah Tabatabaei" <sarah_taba <@t> yahoo.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, October 28, 2011 10:27 AM
Subject: [Histonet] How to save dried antibody?




Dear All,

We have purchased a sheep anti-CGRP antibody for our fluorescent IHC study 
on human tissue and kept it in -20 for a while. It came in liquid but now 
it's all stiff
and looks like it's being crystallized. I cannot pipet any of it. It
has hardened and looks like a piece of glass at bottom of its container. I 
tried to re-suspend it with the same amount of glycerol, but the
antibody doesn't seem to be mixing with it.

Does anyone know how to save this antibody? How can I bring it back to its 
liquid state?

Thank you for your time


Sarah Sadat, DDS


MSc Student, Dental Sciences
McGill University
Montreal QC.

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