[Histonet] Re: Seeking Beta-Amyloid not needing FA pretreatment

[Hobbs, Carl]

Be careful: HIER may expose not only extracellular amyloid plaques but also intracellular APP ( or...is it intracellular A beta ???? ;-))
Eg:  with 6E10.... it is directed against an epitope found in APP and also A beta: if you do Formic acid, only extracellular plaques are positive...after HIER, there is also intracellular positivity.
In my experience.
Some examples of this can be found here, in the image gallery : http://www.immunoportal.com/


Whichever way you go....good luck.

Carl


Carl Hobbs
Histology Manager
Wolfson CARD
School of Biomedical Sciences
Kings College London
Guys Campus
SE1 1UL
Tel: 020 78486813
Fax: 020 78486816
020 78486813