[Histonet] processor stopped - help!

[William Chappell]

Yes, nothing to worry about here (I am assuming an autotechnicon?).  The first paraffin would have dried and sealed the blocks keeping the tissue just fine, however you are probably right in thinking they did not get enough paraffin infiltration.

Try another hour in the second paraffin.  If you have the time, the full 1 1/2 hours is best.

Will

On Oct 24, 2011, at 3:57 PM, Daniela Bodemer wrote:

> Hi all,
> 
> 
> 
> When I arrived the lab this morning I found the processor basket with my
> cassettes stuck between the two paraffin tanks. I suppose it went
> through the first cycle but not the second one, so its missing 1.5 hrs
> of paraffin infusion. Should I put them back in hot paraffin for 1.5
> hours or what can I do to save the samples?
> 
> 
> 
> Thanks for your advice,
> 
> 
> 
> Daniela Bodemer 
> 
> Research Assistant
> 
> Surgical Research, Infection and Immunity
> 
> 
> 
> Murdoch Childrens Research Institute
> 
> The Royal Children's Hospital
> 
> Flemington Road Parkville Victoria 3052 Australia 
> 
> T 03 9345 5930     T (03 9345 4116)     
> 
> E daniela.bodemer <@t> mcri.edu.au <mailto:firstname.surname <@t> mcri.edu.au> 
> 
> www.mcri.edu.au <http://www.mcri.edu.au/> 
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