[Histonet] Ventana's OptiView Detection

[Troutman, Kenneth A]

Hi Matt,

I have worked a bit with this detection kit and my impression is that is extremely sensitive and should be able to boost signal considerably.  I have also been able to shave off an average of 30 minutes off several stains.  (Cyclin D1, which is one of our longer stains at 4 hours is down to 2.5)

You can further enhance the stain (or shorten the time) with the amp kit, but I did not find it necessary.  I thought it was particularly good on nuclear stains.  There is also a good deal of flexibility with the kit and you should be able to get a great stain in less time.

Hope that was helpful.

Ashley Troutman BS, HT(ASCP) QIHC
Immunohistochemistry Supervisor
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4531
Nashville, TN  37232

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Hello All,

Is anyone using Ventana's OptiView detection kit?  If so please let me know what you impression is.

Thank you,

Matt Brooks, BS, HT (ASCP)

Histology Supervisor

InCyte Pathology