[Histonet] ? on frozen tissue artifacts

[Kim Donadio]

Something Ive seen is there are labs out in histoland who just toss that tissue in a bag, put it on ice and think all is well. If this is what your clients are doing then they need to be educated on how to prepare them.Because if this is what they are doing common sense should tell them tissue has water in it, a slow freeze will create more artifacts. Even if they are snap freezing the specimen with alcohol and a dry ice slush, which is better than just putting it in dry ice, some artifacts could happen( I think isopentane and lq nitrogen is still the best method for any tissues imho). The only other things I can think is if somewhere in the process the specimen thaws. Also, without trying to be too much of a wind bag lol...... can you give us the exact procedure your clients have been given by you to snap freeze thier specimens? That might help us help you :)
 
Kim :D


________________________________
From: "histopath101 <@t> gmail.com" <histopath101 <@t> gmail.com>
To: "Esparza, Sandra" <SEsparza <@t> seton.org>; histopath101 <@t> gmail.com
Cc: Histonet <@t> lists.utsouthwestern.edu
Sent: Saturday, October 22, 2011 5:05 PM
Subject: Re: RE: [Histonet] ? on frozen tissue artifacts

I never said these were muscles. These are tumors removed and frozen the day before and sent to us on dry ice. We know not to use frost-free freezers!

On , "Esparza, Sandra" <SEsparza <@t> seton.org> wrote:
> I have never put my muscles in a -20 we use -70 to -80 freezer and have


> not had a problem with artifact. Is your freezer frost free?





> Sandra


> Sandra Esparza HT(ASCP)QIHC


> Lead Technologist


> Dell Children's Medical Center of Central Texas


> 512-324-0000 x87061


> sesparza <@t> seton.org





> -----Original Message-----


> From: histonet-bounces <@t> lists.utsouthwestern.edu


> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of


> histopath101 <@t> gmail.com


> Sent: Friday, October 21, 2011 8:44 AM


> To: Histonet <@t> lists.utsouthwestern.edu


> Subject: [Histonet] ? on frozen tissue artifacts





> I work in a reference lab and some of our frozen sections exhibit severe





> freeze artifact (ice crystals). The client claims it's something we're


> doing and we say they are not freezing them properly. We receive the


> specimens on dry ice and store them in a -20C freezer until we section


> them


> (no more than 1-2 days later). We never allow the tissues to thaw. My


> question: Can freeze artifact occur AFTER properly snap-freezing tissue


> or


> does this artifact ONLY occur during the initial preezing procedure?


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