AW: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus

[Gudrun Lang]

Ventana has a kit for alcian blue.
I wasn't very satisfied with this kit. The staining was faint in comparison
to manual staining. 
Staining temp is at 60 degrees. I'm not sure how the temp influences exactly
the pH of the staining solution, but it may decrease it under the wanted
2,5. So the result is rather a pH1 than pH2,5 alcian blue.
That are just theoretic considerations, never checked. - what do you think?
Gudrun

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Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Scott,
Allison D
Gesendet: Freitag, 21. Oktober 2011 16:01
An: 'histonet <@t> lists.utsouthwestern.edu'
Betreff: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus

Hello to all in histo land.  For those of you that have the ventana special
stains machine, can the alcian blue 2.5 be done on the machine?  I am not
sure if they have kits for this stain.  Also is anyone using the
cryoembedding apparatus from pathology innovations?  Thanks in advance.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital.

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