[Histonet] DRYING TIMES for immuno stains

[William]

Dr. David Tacha at Biocare Medical has done extensive research on this very topic. His findings confirm that the best results (both tissue staying on the slides and IHC reactivity) occur by drying at 37 degrees for 30 minutes to remove any water behind the section slowly, followed by 60 degrees for 30 minutes. Longer times at 60 degrees is just as detrimental as shorter times. 

Hope that helps. Biocare technical support may have copies of his research. 

Will Chappell 

Sent from my iPhone

On Oct 20, 2011, at 8:22 AM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> I always placed the slides in an oven at 60ºC for 30 minutes immediately after they were cut. Just make sure there is no water left behind the section when they go into the oven.
> René J.  
> 
> --- On Thu, 10/20/11, Diana McCaig <dmccaig <@t> ckha.on.ca> wrote:
> 
> 
> From: Diana McCaig <dmccaig <@t> ckha.on.ca>
> Subject: [Histonet] DRYING TIMES for immuno stains
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Thursday, October 20, 2011, 8:40 AM
> 
> 
> Can you tell me how long you let your IHC slides air dry at room
> temperature  and how long you put them in the oven prior to staining?
> 
> 
> 
> Thanks
> 
> Diana
> 
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