[Histonet] Re: (no subject) Miha from SLO

Mon Oct 17 18:26:04 CDT 2011

Hi Miha.

Sounds like pre-freezing artifact. Make sure that the isopentane and
pre-cooled adequately and the specimens are frozen quickly.

On Mon, Oct 17, 2011 at 10:01 AM, <histonet-request <@t> lists.utsouthwestern.edu
> wrote:

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>   1. Re: Looking for recommendations for a slide and cassette
>      labeller system (Linda)
>   2. RE: (no subject) (Tony Henwood)
>   3. RE: Hi histone (crochieresteve <@t> aol.com)
>   4. processing schedule for GI'S (anuradha shrivastava)
>   5. Re: pouring of liquid nitrogen into a cryostat (Tony Reilly)
>   6. Technovit 9100 New (Megan Silas)
>   7. Re: Technovit 9100 New (Jack Ratliff)
>   8. RE: Recommendations for a slide and cassette labeler      system
>      (D'Attilio, Shelley)
>   9. HHV-6 Antibody (McMahon, Loralee A)
>  10. RE: (no subject) (Bea DeBrosse-Serra)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sun, 16 Oct 2011 11:12:55 -0700 (PDT)
> From: Linda <lmdee1 <@t> yahoo.com>
> Subject: Re: [Histonet] Looking for recommendations for a slide and
>        cassette        labeller system
> To: Sheila Adey <sadey <@t> hotmail.ca>,
>        "histonet <@t> lists.utsouthwestern.edu"
>        <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <1318788775.94848.YahooMailNeo <@t> web36506.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi,
>
> I use the Brady system, which has two dimension bar coding.  They also have
> a scan to print, which has a modem for wireless use.  The customer service
> is outstanding.  The price is pretty reasonable too.
>
>
> Linda Dee, BGS, HT(ASCP)
>
>
>
> ________________________________
> From: Sheila Adey <sadey <@t> hotmail.ca>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Saturday, October 15, 2011 7:06 PM
> Subject: [Histonet] Looking for recommendations for a slide and cassette
> labeller system
>
>
> Hello:We are looking into purchasing a slide and cassette labeller system.
> Any advice would be great. Also, if you know which system to stay away from,
> that would be appreciated aswell.ThanksSheila
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 2
> Date: Sun, 16 Oct 2011 22:34:09 +0000
> From: Tony Henwood <AnthonyH <@t> chw.edu.au>
> Subject: RE: [Histonet] (no subject)
> To: "'Miha Tesar'" <sauconym <@t> yahoo.com>,
>        "histonet <@t> lists.utsouthwestern.edu"
>        <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <6D6BD1DE8A5571489398B392A38A7157609F4795 <@t> xmdb02.nch.kids>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Miha,
>
> Are your muscles arising in saline?
> Receive them dry or in cell culture media (eg Hanks).
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Miha Tesar
> Sent: Saturday, 15 October 2011 7:47 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
> Hi!
> I am working in Institute of Phatology Slovenia EU. Recently I have
> problems with muscle biopsy. There are a lots of artefact that I can not
> explain, like little holes in the tissue after the tissue is being frozen.
> We are using the isopentan and the liquid nitrogen for the freezing. Can
> enyone give me some ideas haw to avoid this artefact. How important is
> humidity in laboratory for the good results?
> The next problem is that after I put the tissue on the microscope
> slides after coloring the GTK all the tissue gets wrinkeld. Iam using the
> poly L lisin microscope slides or the Immuno microscope slides.
> Thx in advance!
> Best regards Miha from SLO
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
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> ------------------------------
>
> Message: 3
> Date: Sun, 16 Oct 2011 22:55:41
> From: crochieresteve <@t> aol.com
> Subject: [Histonet] RE: Hi histone
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: 67750d312163f4fa9e5501866fb8429e@[192.168.1.1]
> Content-Type: text/plain; charset="iso-8859-1"
>
> hey how have you been? Do you want to make some easy cash?
> http://t.co/SEgZGeUm You've got to read this story! I will never have to
> worry about the bills being paid on time again!
>
>
>
> ------------------------------
>
> Message: 4
> Date: Sun, 16 Oct 2011 19:33:41 -0400
> From: anuradha shrivastava <shrivastavaanuradha <@t> hotmail.com>
> Subject: [Histonet] processing schedule for GI'S
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <SNT115-DS172B33A303245925541E07ADE70 <@t> phx.gbl>
> Content-Type: text/plain; charset="utf-8"
>
> Hello Histo gurus,
> Could u please advice me on giâ€™s  processing schedule for Leica asp 300.
> thanks in advance.
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 17 Oct 2011 10:01:29 +1000
> From: "Tony Reilly" <Tony_Reilly <@t> health.qld.gov.au>
> Subject: Re: [Histonet] pouring of liquid nitrogen into a cryostat
> To: "Louise Renton" <louise.renton <@t> gmail.com>, "Histonet"
>        <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4E9BFCF9.411C.0039.0 <@t> health.qld.gov.au>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Louise
>
> Not a very safe practice.  I have heard of people using a bag of dry ice
> pellets rested against the knife holder to lower the temperature of the
> knife, both for when the chamber is too warm or the tissue needs to be cut
> at a lower temperature, such as fat.
>
> regards
> Tony
>
>
>
>
> Tony Reilly  B.App.Sc. , M.Sc.
> Chief Scientist, Anatomical Pathology
> Pathology Queensland-PA Laboratory
> _________________________________________________
> Clinical and Statewide Services Division| QueenslandHealth
>
> Level 1, Building 15,Princess Alexandra Hospital
> Ipswich Road,WOOLLOONGABBA  Qld4102
> Ph: 07 3176 2412
> Mob: 0402 139411
> Fax: 07 3176 2930
> Email: tony_reilly <@t> health.qld.gov.au
> Web:  www.health.qld.gov.au/qhcss/
>
>
>
>
> >>> Louise Renton <louise.renton <@t> gmail.com> 10/15/2011 2:59 am >>>
> bad idea - what about splash ups from the chamber? Very scary to
> contemplate
>
> On Fri, Oct 14, 2011 at 6:36 PM, Kaye Ryan <kryan <@t> nfderm.com> wrote:
>
> > Hi Everyone,
> >
> > Has anyone ever heard of pouring liquid nitrogen into the chamber of a
> > cryostat that has warmed up to help cool it down more quickly?  Does
> > this do harm to the chamber or mechanisms of the cryostat?  Any
> > information would be appreciated.
> >
> >
> >
> > Thanks
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> --
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> +27 11 717 2298 (tel & fax)
> 073 5574456 (emergencies only)
> Question: Are rhinos  overweight unicorns?
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> ------------------------------
>
> Message: 6
> Date: Sun, 16 Oct 2011 22:47:48 -0500
> From: Megan Silas <silas1 <@t> illinois.edu>
> Subject: [Histonet] Technovit 9100 New
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>        <CADo6mFWahf4R9nMpQ+fjbKtV1HK8j1cz=L+boLSZZ0FzQQz9rg <@t> mail.gmail.com
> >
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hello,
>
> Has anyone used Technovit 9100 New? If so, what company did you order
> through - is there a distributor in the US? Also, have you found success
> with this in embedding undecalcified bone?
>
> Thanks for any and all advice!
>
> Megan Silas
>
> Megan Silas | megan.r.silas <@t> gmail.com | 847-609-6336
> University of Illinois at Urbana-Champaign | Bioengineering
>
>
> ------------------------------
>
> Message: 7
> Date: Sun, 16 Oct 2011 22:56:31 -0500
> From: Jack Ratliff <ratliffjack <@t> hotmail.com>
> Subject: Re: [Histonet] Technovit 9100 New
> To: Megan Silas <silas1 <@t> illinois.edu>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
>        <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BLU0-SMTP43828AF8CD45E5972E8A27DAEE40 <@t> phx.gbl>
> Content-Type: text/plain; charset="us-ascii"
>
> What is it you are trying to accomplish with staining? Can you tell us more
> about your project? I use Acrylosin SOFT Infiltration and Embedding
> solutions from Dorn and Hart Microedge (just up the road from you in Villa
> Park) for my thin 5 micron section and Acrylosin HARD for ground sections.
> Feel free to ask more questions as needed.
>
>
> Jack
>
>
> Senior Histologist, BioMimetic Therapeutics
> Chairman, Hard Tissue Committee - National Society for Histotechnology
>
>
>
> On Oct 16, 2011, at 10:47 PM, Megan Silas <silas1 <@t> illinois.edu> wrote:
>
> > Hello,
> >
> > Has anyone used Technovit 9100 New? If so, what company did you order
> > through - is there a distributor in the US? Also, have you found success
> > with this in embedding undecalcified bone?
> >
> > Thanks for any and all advice!
> >
> > Megan Silas
> >
> > Megan Silas | megan.r.silas <@t> gmail.com | 847-609-6336
> > University of Illinois at Urbana-Champaign | Bioengineering
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> ------------------------------
>
> Message: 8
> Date: Mon, 17 Oct 2011 07:49:07 -0500
> From: "D'Attilio, Shelley" <SDattili <@t> stormontvail.org>
> Subject: [Histonet] RE: Recommendations for a slide and cassette
>        labeler system
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <
> B7244AC045486C4190DE73F226F432D4221FBD02 <@t> EXCHANGE-VS1.stormontvail.org>
>
> Content-Type: text/plain;       charset="iso-8859-1"
>
> Hi all,
> Sheila asked for recommendations for a slide and cassette labeler system.
>  We use the Leica IPS and IPC printers and we are very happy with their
> reliability and performance.  I believe they are larger than other printers
> but well worth the real estate, in my opinion.  Our printers are interfaced
> to our A/P LIS (WinSurge), but we also have the ability to print slides and
> cassettes as needed through the printing software.  Be advised that our
> installation required that each printer be connected to its own PC and I
> needed to purchase two interfaces from WinSurge, so it wasn't an inexpensive
> project by any means.
>
> I think the Leica labelers are suitable for a high-volume laboratory, but
> may be overkill for a lab with smaller volumes.
>
> Regards,
>
> Shelley D'Attilio MT(ASCP)
> Manager, Chemistry, Cytology and Histology
> Dept. of Pathology and Laboratory Medicine
> Stormont-Vail HealthCare
> Topeka, Kansas
>
>
> NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a
> doctor accepting new patients.  Call (785) 354-5225.
>
>
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> ------------------------------
>
> Message: 9
> Date: Mon, 17 Oct 2011 09:17:33 -0400
> From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
> Subject: [Histonet] HHV-6 Antibody
> To: "Histonet <@t> lists.utsouthwestern.edu"
>        <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <
> EC3287D73321A14799BB96082DE57B470DBC9F <@t> URMCMS2.urmc-sh.rochester.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Hi Histonet.
>
> Can anyone recommend a HHV-6 antibody to use in formalin fixed paraffin
> embedded tissues.
> Preferably ASR or IVD.  But I'll take whatever suggestions.
> Thanks in advance.
>
> Loralee McMahon, HTL (ASCP)
> Immunohistochemistry Supervisor
> Strong Memorial Hospital
> Department of Surgical Pathology
> (585) 275-7210
>
>
> ------------------------------
>
> Message: 10
> Date: Mon, 17 Oct 2011 07:12:45 -0700
> From: Bea DeBrosse-Serra <BDeBrosse-Serra <@t> isisph.com>
> Subject: RE: [Histonet] (no subject)
> To: "'Miha Tesar'" <sauconym <@t> yahoo.com>,
>        "histonet <@t> lists.utsouthwestern.edu"
>        <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <493CAA64F203E14E8823737B9EE0E25F091E912710 <@t> EXCHMB01.isis.local>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Do you coat the muscle biopsies with talcum powder prior to freezing?
>
> Beatrice DeBrosse-Serra HT(ASCP)QIHC
> Isis Pharmaceuticals
> Antisense Drug Discovery
> 1896 Rutherford Road
> Carlsbad, CA 92008
> 760-603-2371
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Miha Tesar
> Sent: Saturday, October 15, 2011 1:47 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
> Hi!
> I am working in Institute of Phatology Slovenia EU. Recently I have
> problems with muscle biopsy. There are a lots of artefact that I can not
> explain, like little holes in the tissue after the tissue is being frozen.
> We are using the isopentan and the liquid nitrogen for the freezing. Can
> enyone give me some ideas haw to avoid this artefact. How important is
> humidity in laboratory for the good results?
> The next problem is that after I put the tissue on the microscope
> slides after coloring the GTK all the tissue gets wrinkeld. Iam using the
> poly L lisin microscope slides or the Immuno microscope slides.
> Thx in advance!
> Best regards Miha from SLO
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> _______________________________________________
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> End of Histonet Digest, Vol 95, Issue 22
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>