[Histonet] Drying oven temps&In-Reply-To=

[Ruppert, Amysue]

For H&E slides, we use the Leica ST5020 autostainer. We have two ovens. The first oven is set for 40 C, 20 min. Then the slide rack is transferred to the second oven at 60 C, 15 min. WE have recently incorporated placing all of our slide racks for H&E in front of a small table top fan for 10-15 minutes prior to going onto the autostainer. This combination works well for us and does a great job of cutting down/ eliminating on the amount of nuclear bubbling artifact. We are not so stringent with our special stain slides. And IHC slides also have their own protocol.
amysue

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