[Histonet] Annexin V IHC staining in mouse xenograft

[awatanabe <@t> tgen.org]

Jan,
Thank you for the input but I did get a lot of suggestions yesterday.
Thank you all.

Aprill Watanabe, B.S.
Research Associate II
Integrated Cancer Genomics Division
Tissue Microarray Center (TMA)
Macroanalyte Analysis & Processing Center (MAPC)
Translational Genomics Research Institute (TGen)
445 North 5th Street
Phoenix, AZ 85004
Main: 602-343-8822
Fax: 602-343-8717
Cell: 602-481-8654
email: awatanabe <@t> tgen.org
website: www.tgen.org



On 10/12/11 8:09 AM, "Jan Shivers" <shive003 <@t> umn.edu> wrote:

>Aprill,
>
>If you send us a list of the exact reagents and incubation
>times/dilutions 
>that you use, someone on Histonet may be able to answer your question.
>
>Jan Shivers
>Univ. of Minnesota
>
>----- Original Message -----
>From: <awatanabe <@t> tgen.org>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Sent: Tuesday, October 11, 2011 12:11 PM
>Subject: [Histonet] Annexin V IHC staining in mouse xenograft
>
>
>Hi histonetters,
>I need some help.  I am running an antibody from Biosensis called Annexin
>V 
>in mouse xenograft brain samples with human glioma tumors treated and
>non-treated.  I have incredible background that I can not get rid of any
>of 
>my samples.  I have used a protein block, rodent tissue block, post
>fixation, short antibody incubation time, short antigen retrieval time,
>very 
>dilute antibody, polymer detection and every combination in between.  I'm
>looking to see if anyone knows anything else to try.  I'm trying to
>rearrange the protocol to put steps in different places to see if that
>works.  At this point I'm not sure what else to try so any ideas would be
>great.
>
>Aprill Watanabe, B.S.
>Research Associate II
>Integrated Cancer Genomics Division
>Tissue Microarray Center (TMA)
>Macroanalyte Analysis & Processing Center (MAPC)
>Translational Genomics Research Institute (TGen)
>445 North 5th Street
>Phoenix, AZ 85004
>Main: 602-343-8822
>Fax: 602-343-8717
>Cell: 602-481-8654
>email: awatanabe <@t> tgen.org
>website: www.tgen.org
>
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