[Histonet] staining for endothelial glycocalyx

[Geoff McAuliffe]

On 10/11/2011 2:21 PM, Featherstone, Annette wrote:
> Does anyone have a staining protocol that will stain sugar structures on the surface of the endothelial cells?? Commonly structures are visable using SEM but I would like to know if it is possible to see it in a paraffin section.
>
> Annette
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>
Many years ago there were some papers on Lanthanum staining the surface 
coat of cells. While these were intended for TEM, I suspect one could 
use them for LM.
I will see if I have some references.

Geoff

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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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