[Histonet] Annexin V IHC staining in mouse xenograft

[awatanabe <@t> tgen.org]

Hi histonetters,
I need some help.  I am running an antibody from Biosensis called Annexin V in mouse xenograft brain samples with human glioma tumors treated and non-treated.  I have incredible background that I can not get rid of any of my samples.  I have used a protein block, rodent tissue block, post fixation, short antibody incubation time, short antigen retrieval time, very dilute antibody, polymer detection and every combination in between.  I'm looking to see if anyone knows anything else to try.  I'm trying to rearrange the protocol to put steps in different places to see if that works.  At this point I'm not sure what else to try so any ideas would be great.

Aprill Watanabe, B.S.
Research Associate II
Integrated Cancer Genomics Division
Tissue Microarray Center (TMA)
Macroanalyte Analysis & Processing Center (MAPC)
Translational Genomics Research Institute (TGen)
445 North 5th Street
Phoenix, AZ 85004
Main: 602-343-8822
Fax: 602-343-8717
Cell: 602-481-8654
email: awatanabe <@t> tgen.org
website: www.tgen.org