[Histonet] 10% sucorse in Sodium Cacodylate

[Geoff McAuliffe]

On 10/10/2011 1:37 PM, sarah Tabatabaei wrote:
> Hi histoneters,
>
> I have a question regarding Sodium Cacodylate.
> In our lab, after fixing our human tissue, we usually leave it in 10% sucrose in double distilled water for a couple of days to remove all the fixatives out of it. Recently, I  used a solution of 10% sucrose in 0.1 ml Sodium Cacodylate, instead of the plain distilled water thing. I have a feeling I have ruined them for IHC. right? Can someone tell me what I have done to my tissue? I am supposed to do immunohistochemistry and some histology on these tissues.
>
>
> Looking forward to hear from you
>
>
> Sarah
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
Why do you think the specimens are ruined?
Certainly no problem with the histology, cacodylate buffer has been used 
for many years for EM. Its use has declined as less-toxic alternatives 
have been found.
As for immuno, try it, what have you got to lose?
And why did you use cacodylate in the first place?

Geoff

-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
**********************************************