[Histonet] ovarian cryosections

[Tony Henwood]

Carmen,

If the tissues have been fixed in formalin, then this would not be a problem, but if not, why not freeze the tissues in OCT (or similar) and store it at -70oC before cryotomy, rather than placing it in a sucrose solution?

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carmen Maria Garcia Pascual
Sent: Tuesday, 11 October 2011 1:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] ovarian cryosections


Hello everybody:
I am here requesting your help another time ;) I want to make ovarian cryosections and I want to know if there is any problem if I leave the ovaries in 30% sucrose at 4ºC overnigth.
Thank you very much.

Kind regards,
Carmen


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