[Histonet] RE:Leaking Jung XL stainer
Menon Dhanya
Dhanya.Menon <@t> petermac.org
Mon Oct 10 17:12:36 CDT 2011
Hello,
We had a similar problem with our jung autostainer Xl after we had it serviced, but was fixed after we adjusted the water pressure from the tap....hope this helps
2010 Metropolitan Health Service of the Year
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Subject: Histonet Digest, Vol 95, Issue 14
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Today's Topics:
1. Green counterstain problems (Shea's)
2. Leaking Jung XL stainer (Shelly Coker)
3. RE: Cold Plates - Source? (Tom McNemar)
4. RE: Cold Plates - Source? (Hannen, Valerie)
5. EDTA (Jose Luis Palazon Fernandez)
6. (no subject) (Nat Nunut)
7. ovarian cryosections (Carmen Maria Garcia Pascual)
8. Re: Leaking Jung XL stainer (Rene J Buesa)
9. Re: Leaking Jung XL stainer (Paula Pierce)
----------------------------------------------------------------------
Message: 1
Date: Sun, 9 Oct 2011 20:14:28 -0400
From: "Shea's" <jshea121 <@t> roadrunner.com>
Subject: [Histonet] Green counterstain problems
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4ECF7C3CEF464FD79473F0B6436C7B00 <@t> JoannePC>
Content-Type: text/plain; charset="iso-8859-1"
This happens to us after extended use. Add about 5 drops/100ml of glacial acetic acid to the light green working solution.
------------------------------
Message: 2
Date: Sun, 9 Oct 2011 20:38:53 -0700 (PDT)
From: Shelly Coker <sccrshlly <@t> yahoo.com>
Subject: [Histonet] Leaking Jung XL stainer
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1318217933.71551.YahooMailNeo <@t> web113720.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I have a refurbished Jung XL autostainer that has been great for the past 5 years. Last week the fan seized up and is being replaced. While moving around the machine, I believe that inadvertently we somehow did something to cause the water hose to start leaking. We tried getting a new hose, but it is still leaking. Any suggestions?
Thanks in advance!
------------------------------
Message: 3
Date: Mon, 10 Oct 2011 06:13:50 -0400
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: [Histonet] RE: Cold Plates - Source?
To: "'Monfils, Paul'" <PMonfils <@t> Lifespan.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E9A90E28259D2F4E84308C5E8EA8F7B43AF7EA0DAB <@t> lmhs-exchange.lmhealth.org>
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Check out the Histo-Cool trays http://www.marketlabinc.com/product.asp?P_ID=4854
You can get them from several places. They stay cold all day.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils, Paul
Sent: Friday, October 07, 2011 4:16 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cold Plates - Source?
Hello All,
Does anyone know where Tissue-Tek cold plates can be purchased? They
are plastic, black on the bottom, white on top, gel filled, for chilling
blocks. I got them from Cardinal Healthcare a couple of years ago, but
I don't see it in their catalog now. Or, if no longer available, a
comparable other product? I know I can use a pan of ice, but I prefer
something like this if available. Thanks.
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This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
------------------------------
Message: 4
Date: Mon, 10 Oct 2011 05:59:50 -0400
From: "Hannen, Valerie" <Valerie.Hannen <@t> parrishmed.com>
Subject: [Histonet] RE: Cold Plates - Source?
To: "'Monfils, Paul'" <PMonfils <@t> Lifespan.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <450B7A81EDA0C54E97C53D60F00776C322A4AC0F29 <@t> isexstore03>
Content-Type: text/plain; charset="us-ascii"
You can still get them from Cardinal Health.
The catalog number should be M7410-10. There are 6 to a pack.
Hope this helps.
Valerie Hannen, MLT(ASCP), HTL,SU(FL)
Parrish Medical Center
Titusville, Florida
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils, Paul
Sent: Friday, October 07, 2011 4:16 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cold Plates - Source?
Hello All,
Does anyone know where Tissue-Tek cold plates can be purchased? They are plastic, black on the bottom, white on top, gel filled, for chilling blocks. I got them from Cardinal Healthcare a couple of years ago, but I don't see it in their catalog now. Or, if no longer available, a comparable other product? I know I can use a pan of ice, but I prefer something like this if available. Thanks.
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Message: 5
Date: Mon, 10 Oct 2011 13:34:09 +0200 (CEST)
From: Jose Luis Palazon Fernandez <jluis.palazon <@t> icman.csic.es>
Subject: [Histonet] EDTA
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <146077420.19002.1318246449245.JavaMail.tomcat <@t> cedric>
Content-Type: text/plain; charset=iso-8859-1
Dear List members
Greetings
I would like to ask you some questions about the use of EDTA as demineratizing agent.
I have to do histology and histochemistry (including metallothioneins) in fish larvae preserved in Bouins solution.
1) First of all I would like to know what is the best formula (or the one you recomend) to prepare the EDTA solution, as well as the importance of maintaining a given pH and if it is necessary to put the samples in the refrigerator during the demineralization process.
2) Is EDTA recomended only for certain fixatives, or It can be used with any fixative, including Bouins
3) When using Bouins solution as fixative, many books recommend not to wash in water but instaed to remove the excess of picric acid using several washes in etanol 70%, on the other hand, I´ve read that EDTA precipitates in the presence of alcohols. What do you recomend in this case?
4) Do you think that the demineralization procedure could alter or affect the detection of metallothioneins in the tissues?
Many thanks in advance
Best regards
José Luis
Dr. José Luis Palazón Fernández
Instituto de Investigaciones Científicas
Universidad de Oriente
Boca del Rio-Isla Margarita-Venezuela
Direccion actual: Instituto de Ciencias Marinas de Andalucia-CSIC
Campus universitario Rio San Pedro, 11510, Puerto Real, Cádiz, España
email: jluis.palazon <@t> icman.csic.es; jose.palazon <@t> ne.udo.edu.ve
tlf: +34-956832612; fax: +34-956834701; cell: +34-600487100
------------------------------
Message: 6
Date: Mon, 10 Oct 2011 13:43:48 +0000
From: Nat Nunut <nunut86 <@t> hotmail.com>
Subject: [Histonet] (no subject)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <COL124-W48BEE396906881997AA7C1B5FD0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Hi everybody!
I'm interested in making a Prussian Blue (Potassium ferrocyanide) staining to highlight micro vascular infarctus in human brains.
In the protocol I have, it's written that a nitric acid pre-treatment is necessary.
I would like to know:
1) What nitric acid is used for in histological staining?
2) What is its function in histological staining?
I hope you can help me.
Thanks
Natalia
------------------------------
Message: 7
Date: Mon, 10 Oct 2011 16:10:25 +0200 (CEST)
From: "Carmen Maria Garcia Pascual"<Carmen.M.Garcia <@t> uv.es>
Subject: [Histonet] ovarian cryosections
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <5355778534carmaga6 <@t> uv.es>
Content-Type: text/plain; charset="ISO-8859-1"
Hello everybody:
I am here requesting your help another time ;)
I want to make ovarian cryosections and I want to know if there is any
problem if I leave the ovaries in 30% sucrose at 4ºC overnigth.
Thank you very much.
Kind regards,
Carmen
------------------------------
Message: 8
Date: Mon, 10 Oct 2011 07:11:08 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Leaking Jung XL stainer
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, Shelly Coker
<sccrshlly <@t> yahoo.com>
Message-ID:
<1318255868.96684.YahooMailClassic <@t> web65707.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Check the connection from the hose into de stainer. It may be broken/stressed.
René J.
--- On Sun, 10/9/11, Shelly Coker <sccrshlly <@t> yahoo.com> wrote:
From: Shelly Coker <sccrshlly <@t> yahoo.com>
Subject: [Histonet] Leaking Jung XL stainer
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Sunday, October 9, 2011, 11:38 PM
I have a refurbished Jung XL autostainer that has been great for the past 5 years. Last week the fan seized up and is being replaced. While moving around the machine, I believe that inadvertently we somehow did something to cause the water hose to start leaking. We tried getting a new hose, but it is still leaking. Any suggestions?
Thanks in advance!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Mon, 10 Oct 2011 07:20:21 -0700 (PDT)
From: Paula Pierce <contact <@t> excaliburpathology.com>
Subject: Re: [Histonet] Leaking Jung XL stainer
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1318256421.4244.YahooMailNeo <@t> web1116.biz.mail.sk1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi,
as stainers age, the hoses can harden and crack. I have replaced all hoses on many.
If one is bad, they all are. By replacing one, you may have put stress on the next.
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
________________________________
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>; Shelly Coker <sccrshlly <@t> yahoo.com>
Sent: Monday, October 10, 2011 9:11 AM
Subject: Re: [Histonet] Leaking Jung XL stainer
Check the connection from the hose into de stainer. It may be broken/stressed.
René J.
--- On Sun, 10/9/11, Shelly Coker <sccrshlly <@t> yahoo.com> wrote:
From: Shelly Coker <sccrshlly <@t> yahoo.com>
Subject: [Histonet] Leaking Jung XL stainer
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Sunday, October 9, 2011, 11:38 PM
I have a refurbished Jung XL autostainer that has been great for the past 5 years. Last week the fan seized up and is being replaced. While moving around the machine, I believe that inadvertently we somehow did something to cause the water hose to start leaking. We tried getting a new hose, but it is still leaking. Any suggestions?
Thanks in advance!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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