[Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4%

[Jenny Vega]

Thanks. Thank God that the company that supplied the product contacted her
and set her straight by telling her the same information you guys have told
me. I even pointed the chapter from the Frieda Carson's book again, when it
clearly says they are the same thing. Now she understood! Thank God I was
getting frustrated because she did not agreed with me :(.

On Thu, Oct 6, 2011 at 9:12 AM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> Jenny:
> Formaldehyde is the GAS methanal that dissolves in water at a maximum
> concentration between 37-40% vol./vol. To prevent its polymerization, small
> amounts of mathanol (an alcohol)are added.
> The 10% solution formaldehyde contains between 3.7 and 4.0% of formaldehyde
> and is called "formalin" in many countries and "formol" in Europe.
> So formalin is a 3.7 to 4.0% solution of formaldehyde.
> So a 10% formalin = 3.7-4.0% formaldehyde.
> NBF is prepared by added phosphate salts to prevent the formalin to acidify
> and is used to fix tissues.
> It is a pitty that your supervisor has so little knowledge about the area
> she is supposed to supervise, but I am not do not know why, but I am
> not. surprised.
> To your question: SHE is wrong, really wrong!
> René J.
>
>
> --- On *Wed, 10/5/11, Jenny Vega <histotech411 <@t> gmail.com>* wrote:
>
>
> From: Jenny Vega <histotech411 <@t> gmail.com>
> Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB
> formaldehyde 3.7 to 4%
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Wednesday, October 5, 2011, 7:03 PM
>
> Ok I want to know the diferrence between Neutral Buffered Formalin 10% and
> NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same
> thing and in the book from Frieda Carson it says that but my supervisor
> swears they are different chemicals. In the laboratory she has used 10%
> neutral buffered formalin all the time, but when we ran out of it  we were
> sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB
> formaldehyde 3.7 to 4% once again. She says that 10% NBF is more
> concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are
> going to  putrefied if they are put in that solution.
>
> Am I wrong or is she wrong? thanks
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