[Histonet] Tissue left in processor

[Sherwood, Margaret]

I don't think you want to leave it in the processor for 2 hours.  Even at the
embedding center, if someone can't come to help orient the tissue, we then take
the cassettes out of the paraffin station and leave on a paper towel.  Have done
that for sometimes 2-3 days.

Peggy




Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Charles, Roger
Sent: Thursday, October 06, 2011 10:05 AM
To: Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] Tissue left in processor

Hi All,
Is there any standard on how long tissue cassettes can remain in the processor
after processing before the tissue is subjected to unwanted outcomes?  And if so
what type of artifacts can one expect from tissue that was in the processor in
molten paraffin for 2 hours after the processing was completed?
Thanks so much.
Roger

Roger Charles| Microbiologist II
Pennsylvania Veterinary Laboratory
2305 North Cameron Street | Harrisburg, PA 17110
Phone: 717.787.8808 | Fax: 717.772.3895
www.agriculture.state.pa.us<http://www.agriculture.state.pa.us>

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