[Histonet] DAPI question

[Carmen Maria Garcia Pascual]

Good morning:

I am Carmen Garcia from the Faculty of Medicine of the University of 
Valencia, Spain. I usually use your vectashield mounting medium for 
fluorescence with DAPI (H-1200) and I never have any problem.
But now I am working with 50um frozen sections and the problem that I 
have is that the DAPI staining does not arrive to the bottom of the 
section. Do you have any sugesstion of what can I do? (I already have 
the sections permeabilized with triton x-100) I have thougth to 
incubate with the mounting medium some hours at 4ºC or put some 
mounting medium in the antibody diluent and incubate...

any suggestion would be really welcome.
Thank you so much,
Kind regards,

Carmen