[Histonet] Paraffin sections for molecular assays

[Amos Brooks]

Hi,
    You will want to do more than just wiping it down. You should use
something specifically designed to clean such contamination like RNAase away
(Invitrogen if memory serves). You'll want to wipe down any surfaces that
come in contact with the block, sections or your hands when sectioning.
Don't forget your forceps & probe or whatever you use and wear gloves. Just
because I'm obsessive compulsive, I dip the block it's self into the stuff.
It is best to float the sections on molecular biology grade water or
de-ionized water that has been treated with DEPC. Molecular water is cheaper
and don't stink like DEPC!

Good luck,
Amos

On Tue, Oct 4, 2011 at 1:00 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:

> Message: 2
> Date: Tue, 4 Oct 2011 16:44:17 +0000
> From: "Crowell, Thomas" <thomas.crowell <@t> novartis.com>
> Subject: [Histonet] Paraffin sections for molecular assays
> To: "histonet <@t> lists.utsouthwestern.edu"
>        <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
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> BC3645844188C945830E826F2FF0977D014B59FA <@t> 023-CH1MPN1-022.023d.mgd.msft.net
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> Dear Histonetters,
>
> Can you tell me what procedures you use to prevent DNA/RNA contamination of
> tissue sections when handling multiple samples.  Do you just wipe down blade
> holder and blade (or use a new blade) between samples, or do you use more
> stringent cleaning?
>
>
> Thomas Crowell
>