[Histonet] RE: Slide/Block retention
Gilchrist, Eric
gilchrie <@t> DENTISTRY.UNC.EDU
Tue Oct 4 11:05:10 CDT 2011
CLIA-88 requirements can be found here: http://wwwn.cdc.gov/clia/regs/subpart_j.aspx. Local and state requirements may vary.
Eric Gilchrist, Supervisor
UNC Oral and Maxillofacial Pathology Laboratory
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, October 04, 2011 11:08 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 95, Issue 4
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Today's Topics:
1. Re: Slide/Block Retention (Richard Cartun)
2. Cpt Charge (Anita Buchiane)
3. RE: Atlas of laboratory mouse histology (Hinsinger Julie)
4. RE: Cpt Charge (Weems, Joyce)
5. RE: decal question (Sarah Dysart)
6. RE: Cpt Charge (Setlak, Lisa)
7. RE: decal question (Bernice Frederick)
8. RE: decal question (Houston, Ronald)
9. RE: decal question (Patsy Ruegg)
10. Re: Slide/Block Retention (Bob Richmond)
11. RE: Atlas of laboratory mouse histology (Andrea Marion)
12. RE: Re: Slide/Block Retention (Blazek, Linda)
13. HP controls (Marcia Funk)
14. Atlas of laboratory mouse histology (Amos Brooks)
15. RE: decal question (Jennifer MacDonald)
16. RE: decal question (Edwards, Richard E.)
17. Re: Von Kossa Stain for calcium (Sherwood, Margaret)
18. Green counterstain problems (rgrow <@t> bmnet.com)
19. RELIA Histology Careers Bulletin Special Edition for Managers
and Supervisors 10-4-2011 (Pam Barker)
20. RE: Green counterstain problems (Bell, Lynne)
----------------------------------------------------------------------
Message: 1
Date: Mon, 03 Oct 2011 13:20:42 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: Re: [Histonet] Slide/Block Retention
To: <histonet <@t> lists.utsouthwestern.edu>, "Michael J Dessoye"
<mjdessoye <@t> wvhcs.org>
Message-ID: <4E89B6AA.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII
We currently keep slides and paraffin blocks (surgical pathology) for 17 years; I would keep more, but laboratory administration keeps tell me to discard because we don't have sufficient storage space. It's not unusual for me to receive a request for a block(s) from the 1990's for IHC, molecular, or genetic testing especially now that we have entered the arena for "Personalized Medicine". Also, I find myself re-testing specimens from the 1990's and 2000's with new generation antibodies, probes, and detection systems.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
>>> "Dessoye, Michael J" <mjdessoye <@t> wvhcs.org> 10/3/2011 9:50 AM >>>
Hello Histonet,
What policy is everyone following for slide and block retention? We are
not CAP, however we currently keep slides and blocks for 10 years. My
director wants to decrease that period to 2 years, which is the Joint
Commission standard. I would like to keep 10 years because we
frequently are asked to send slides for consult and review that are
several years old. It is also helpful when looking for hard-to-find
control tissue. However these reasons are apparently not sufficient.
I'm leaning towards keeping the 10 year policy, but I need additional
justification. Or does anyone think the 2 year period is sufficient?
Thanks,
Mike
Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health
Care System | mjdessoye <@t> wvhcs.org <mailto:mjdessoye <@t> wvhcs.org> |
575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax:
570-552-1526
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Message: 2
Date: Mon, 3 Oct 2011 17:24:16 +0000
From: Anita Buchiane <abuchiane <@t> bmhvt.org>
Subject: [Histonet] Cpt Charge
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4034E71604330C4B8E10D1538DFB2455CD38 <@t> BMHEXCH02.bmhvt.org>
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Can we still only bill 88342 per antibody /per part or has that been changed?
For instance: If we do a Calponin and an Actin on 3 blocks from one part do we charge 88342 x 2 or x 6?
I thought I remember reading that it was changed to allow for charging per slide but I can not find the reference.
Thanks
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Message: 3
Date: Mon, 3 Oct 2011 13:27:52 -0400
From: "Hinsinger Julie" <julie.hinsinger <@t> umontreal.ca>
Subject: [Histonet] RE: Atlas of laboratory mouse histology
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<070FB8DB1EFA214292C54DCBECEB27CF02566A8F <@t> MAPIUDEM2.sim.umontreal.ca>
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Just to let you know about the first answers:
- There is an online atlas that was brought to my attention :
http://www.mouseatlas.org/data/mouse/libraries/SM202 <http://www.mouseatlas.org/data/mouse/libraries/SM202>
- And most referred book was Atlas of Mouse Development by Matthew H. Kaufman with a revised edition, printed in 2002. (Good for mouse embryo stages)
I might have another question: As for adult organs, is there a general histology atlas you refer to? In our lab, we use "Pathology of the Mouse" Robert R. Maronpot <http://www.amazon.ca/s?_encoding=UTF8&search-alias=books-ca&field-author=Robert%20R.%20Maronpot>
As we work on both human and mouse tissues, I have found a new book that might be interesting "Comparative Anatomy and Histology: A Mouse and Human Atlas" Have you heard about it? It will be released in December 2011.
http://www.amazon.ca/Comparative-Anatomy-Histology-Expert-Consult/dp/0123813611/ref=sr_1_1?s=books&ie=UTF8&qid=1317661829&sr=1-1
It aims at the new mouse investigator as well as medical and veterinary pathologists who need to expand their knowledge base into comparative anatomy and histology. It guides the reader through normal mouse anatomy and histology using direct comparison to the human. The side by side comparison of mouse and human tissues highlight the unique biology of the mouse, which has great impact on the validation of mouse models of human disease.
Seems great! I don't know...
Julie
De : Hinsinger Julie
Envoy? : 3 octobre 2011 11:41
? : 'histonet <@t> lists.utsouthwestern.edu'
Objet : Atlas of laboratory mouse histology
Does anyone know of an atlas of mouse histology?
Thanks for your comments
Julie Hinsinger
Histology Facility Manager
IRIC - Pav. M. Coutu - Local 3440
2950, chemin polytechnique - Montr?al (Qc) H3T 1J4
------------------------------
Message: 4
Date: Mon, 3 Oct 2011 13:54:23 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: [Histonet] RE: Cpt Charge
To: Anita Buchiane <abuchiane <@t> bmhvt.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<92AD9B20A6C38C4587A9FEBE3A30E1640827B749CC <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
You can now charge per block.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anita Buchiane
Sent: Monday, October 03, 2011 13:24
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cpt Charge
Can we still only bill 88342 per antibody /per part or has that been changed?
For instance: If we do a Calponin and an Actin on 3 blocks from one part do we charge 88342 x 2 or x 6?
I thought I remember reading that it was changed to allow for charging per slide but I can not find the reference.
Thanks
_______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________
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Message: 5
Date: Mon, 3 Oct 2011 17:55:04 +0000
From: Sarah Dysart <sdysart <@t> mirnarx.com>
Subject: RE: [Histonet] decal question
To: Patsy Ruegg <pruegg <@t> ihctech.net>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8A70A9B2ECDD084DACFE6C59FCF86D5079FA <@t> SN2PRD0702MB110.namprd07.prod.outlook.com>
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Formalin? Isn't all decal acid decal?
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, October 03, 2011 11:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decal question
Hi Everyone,
I have a new student taking course work at UND and using my lab for her
practical clinical site. She took a test on decal today and there was a
question we didn't know the answer to.
What fixative should not be used for acid decalcification? Would it be
osmium tetroxide?
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
web site <http://www.ihctech.net> www.ihctech.net
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------------------------------
Message: 6
Date: Mon, 3 Oct 2011 13:01:04 -0500
From: "Setlak, Lisa" <LSetlak <@t> childrensmemorial.org>
Subject: [Histonet] RE: Cpt Charge
To: "'Weems, Joyce'" <JWeems <@t> sjha.org>, Anita Buchiane
<abuchiane <@t> bmhvt.org>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<7111DB39D045004C9CF29E79C71B28BC1093D4D479 <@t> CMHEXCC01MBX.childrensmemorial.org>
Content-Type: text/plain; charset="us-ascii"
Where we can find a reference to this.... I know I'll get questions when I change it?
Thanks,
Lisa
Children's Memorial Hospital
Chicago, Il
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Monday, October 03, 2011 12:54 PM
To: Anita Buchiane; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Cpt Charge
You can now charge per block.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anita Buchiane
Sent: Monday, October 03, 2011 13:24
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cpt Charge
Can we still only bill 88342 per antibody /per part or has that been changed?
For instance: If we do a Calponin and an Actin on 3 blocks from one part do we charge 88342 x 2 or x 6?
I thought I remember reading that it was changed to allow for charging per slide but I can not find the reference.
Thanks
_______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________
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------------------------------
Message: 7
Date: Mon, 3 Oct 2011 18:17:59 +0000
From: Bernice Frederick <b-frederick <@t> northwestern.edu>
Subject: RE: [Histonet] decal question
To: Patsy Ruegg <pruegg <@t> ihctech.net>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<62C639732D3F274DACED033EBDF6ADAF1E17A1B4 <@t> evcspmbx3.ads.northwestern.edu>
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Ann Preece states acid decal uses aqueous solutions od either formic,nitric or trichloroacetic acid. Other methods mentioned are Ion-exchange resin,electrical ionization and chelation. The histo bible!
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, October 03, 2011 11:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decal question
Hi Everyone,
I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to.
What fixative should not be used for acid decalcification? Would it be osmium tetroxide?
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net web site <http://www.ihctech.net> www.ihctech.net
This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.
_______________________________________________
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------------------------------
Message: 8
Date: Mon, 3 Oct 2011 18:25:32 +0000
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: RE: [Histonet] decal question
To: 'Patsy Ruegg' <pruegg <@t> ihctech.net>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E5D0CD2352E46545A0C0EBE308CCCE5317B6A3 <@t> L1PERDWXMB01.childrensroot.net>
Content-Type: text/plain; charset="us-ascii"
Alcoholic formalin or 70% ethanol as, quoting Gayle Callis, "alcohol can slow or prevent decalcification"
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, October 03, 2011 12:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decal question
Hi Everyone,
I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to.
What fixative should not be used for acid decalcification? Would it be osmium tetroxide?
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net web site <http://www.ihctech.net> www.ihctech.net
This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.
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Message: 9
Date: Mon, 3 Oct 2011 12:25:52 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] decal question
To: "'Sarah Dysart'" <sdysart <@t> mirnarx.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <7104CA2AA7E64D859B3B1084AD323E5B <@t> Patsyoffice>
Content-Type: text/plain; charset="us-ascii"
I think I miss phrased this question, we thought they were asking what
fixative should never be used if you are planning to do acid decal after
fixation? We still think it must be osmium????
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.
-----Original Message-----
From: Sarah Dysart [mailto:sdysart <@t> mirnarx.com]
Sent: Monday, October 03, 2011 11:55 AM
To: Patsy Ruegg; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] decal question
Formalin? Isn't all decal acid decal?
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, October 03, 2011 11:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decal question
Hi Everyone,
I have a new student taking course work at UND and using my lab for her
practical clinical site. She took a test on decal today and there was a
question we didn't know the answer to.
What fixative should not be used for acid decalcification? Would it be
osmium tetroxide?
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
web site <http://www.ihctech.net> www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.
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------------------------------
Message: 10
Date: Mon, 3 Oct 2011 14:30:26 -0400
From: Bob Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Slide/Block Retention
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAOKsRH43itNP+YL_fgqq_0VGaoLbCU1=f18Y4-qMS2vTKUPVyg <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
I don't care what the herrn inshpektors say, the fact is that patient
care - with the rapid growth of genetic and other molecular techniques
- I think 10 years is the minimum for retaining paraffin blocks, and
I'd be a lot more comfortable with 20. In the future I expect we'll be
retrieving blocks to do studies on members of patients' families.
And if your kid flunks out of college, I reckon you can sue the OB
that delivered him - so that's >20 years for placentas.
An issue rarely mentioned: when a tissue lab closes, everything is
often discarded. We need requirements for tissue retention when labs
close. I have no idea how this could be done. But as regulations
increase and our economy declines, there'll be a lot of tissue labs
closing in the next decade.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 11
Date: Mon, 3 Oct 2011 14:34:07 -0500
From: "Andrea Marion" <amario3 <@t> uic.edu>
Subject: [Histonet] RE: Atlas of laboratory mouse histology
To: histonet <@t> lists.utsouthwestern.edu
Cc: julie.hinsinger <@t> umontreal.ca
Message-ID:
<0c00e9909cf1a85757b0f687b639347f.squirrel <@t> webmail.uic.edu>
Content-Type: text/plain;charset=iso-8859-1
Hi Julie -
Other than the ones you already found, I have two (okay 3) more
recommendations:
1- Theiler's classic The House Mouse: Atlas of Embryonic Development has a
great histology of mouse embryos. I use it side-by-side with the Kaufmann
text for embryo histology. It's freely available for download, which is
very handy:
http://www.emouseatlas.org/Atlas/Theiler_book_download.html
2- There is a quick online guide for adult mouse histology at
http://ctrgenpath.net/static/atlas/mousehistology/Windows/introduction.html
It's a nice page for quickly checking something, but there is not a lot of
in depth coverage.
3 - I also suggest searching on the mouse research listserv, I've seen it
discussed there before and a quick search turned up a number of
recommendations:
http://www.informatics.jax.org/lyris-cgi/lyris.pl?visit=mgi-list
Good luck,
Andrea
Andrea Marion
Graduate Student
University of Illinois at Chicago
[Histonet] RE: Atlas of laboratory mouse histology
Hinsinger Julie julie.hinsinger <@t> umontreal.ca
Mon Oct 3 12:27:52 CDT 2011
Previous message: [Histonet] RE: Cpt Charge
Next message: [Histonet] decal question
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
Just to let you know about the first answers:
- There is an online atlas that was brought to my attention :
http://www.mouseatlas.org/data/mouse/libraries/SM202
<http://www.mouseatlas.org/data/mouse/libraries/SM202>
- And most referred book was Atlas of Mouse Development by
Matthew H. Kaufman with a revised edition, printed in 2002. (Good for
mouse embryo stages)
I might have another question: As for adult organs, is there a general
histology atlas you refer to? In our lab, we use "Pathology of the Mouse"
Robert R. Maronpot
<http://www.amazon.ca/s?_encoding=UTF8&search-alias=books-ca&field-author=Robert%20R.%20Maronpot>
As we work on both human and mouse tissues, I have found a new book that
might be interesting "Comparative Anatomy and Histology: A Mouse and Human
Atlas" Have you heard about it? It will be released in December 2011.
http://www.amazon.ca/Comparative-Anatomy-Histology-Expert-Consult/dp/0123813611/ref=sr_1_1?s=books&ie=UTF8&qid=1317661829&sr=1-1
It aims at the new mouse investigator as well as medical and veterinary
pathologists who need to expand their knowledge base into comparative
anatomy and histology. It guides the reader through normal mouse anatomy
and histology using direct comparison to the human. The side by side
comparison of mouse and human tissues highlight the unique biology of the
mouse, which has great impact on the validation of mouse models of human
disease.
Seems great! I don't know...
Julie
De : Hinsinger Julie
Envoy? : 3 octobre 2011 11:41
? : 'histonet <@t> lists.utsouthwestern.edu'
Objet : Atlas of laboratory mouse histology
Does anyone know of an atlas of mouse histology?
Thanks for your comments
Julie Hinsinger
Histology Facility Manager
IRIC - Pav. M. Coutu - Local 3440
2950, chemin polytechnique - Montr?al (Qc) H3T 1J4
------------------------------
Message: 12
Date: Mon, 3 Oct 2011 15:50:15 -0400
From: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
Subject: RE: [Histonet] Re: Slide/Block Retention
To: 'Bob Richmond' <rsrichmond <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5A2BD13465E061429D6455C8D6B40E39127401B36E <@t> IBMB7Exchange.digestivespecialists.com>
Content-Type: text/plain; charset="us-ascii"
There is an inspection question that addresses what happens to material if a lab closes.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Monday, October 03, 2011 2:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Slide/Block Retention
I don't care what the herrn inshpektors say, the fact is that patient
care - with the rapid growth of genetic and other molecular techniques
- I think 10 years is the minimum for retaining paraffin blocks, and
I'd be a lot more comfortable with 20. In the future I expect we'll be
retrieving blocks to do studies on members of patients' families.
And if your kid flunks out of college, I reckon you can sue the OB
that delivered him - so that's >20 years for placentas.
An issue rarely mentioned: when a tissue lab closes, everything is
often discarded. We need requirements for tissue retention when labs
close. I have no idea how this could be done. But as regulations
increase and our economy declines, there'll be a lot of tissue labs
closing in the next decade.
Bob Richmond
Samurai Pathologist
Knoxville TN
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Mon, 03 Oct 2011 16:20:34 -0400
From: "Marcia Funk" <FUNKM <@t> mercyhealth.com>
Subject: [Histonet] HP controls
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4E89D2C2.E948.00AC.1 <@t> mercyhealth.com>
Content-Type: text/plain; charset=US-ASCII
We are having difficult with the HP control for IHC staining - If you
are purchasing controls or if you have controls that you might share
I would really appreciate your help. Thanks so much -Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-428-7907
------------------------------
Message: 14
Date: Mon, 3 Oct 2011 17:27:28 -0400
From: Amos Brooks <amosbrooks <@t> gmail.com>
Subject: [Histonet] Atlas of laboratory mouse histology
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAC95ki8fiTp-N9h_wai7p7HCT+2OzEq950ePwPdAJb-KHuTO=A <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi,
I would be interested in this reference as well. Please post to the group if
you have any suggestions.
Amos
On Mon, Oct 3, 2011 at 1:00 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:
> Message: 10
> Date: Mon, 3 Oct 2011 11:40:55 -0400
> From: "Hinsinger Julie" <julie.hinsinger <@t> umontreal.ca>
> Subject: [Histonet] Atlas of laboratory mouse histology
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> 070FB8DB1EFA214292C54DCBECEB27CF02566A13 <@t> MAPIUDEM2.sim.umontreal.ca>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone know of an atlas of mouse histology?
>
> Thanks for your comments
>
>
>
> Julie Hinsinger
>
> Histology Facility Manager
>
------------------------------
Message: 15
Date: Tue, 4 Oct 2011 04:46:50 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: RE: [Histonet] decal question
To: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF0F04E57D.1026C4C4-ON8825791F.00409D53-8825791F.0040B68C <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"
Was there more to the question? You would not want to use any fixatives
containing metals, such as mercury, if you will be doing end-point
decalcification determination using x-rays.
"Patsy Ruegg" <pruegg <@t> ihctech.net>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
10/03/2011 11:27 AM
To
"'Sarah Dysart'" <sdysart <@t> mirnarx.com>,
<histonet <@t> lists.utsouthwestern.edu>
cc
Subject
RE: [Histonet] decal question
I think I miss phrased this question, we thought they were asking what
fixative should never be used if you are planning to do acid decal after
fixation? We still think it must be osmium????
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
This email is confidential and intended solely for the use of the
Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that
is
privileged & confidential within the meaning of applicable law.
Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited.
If
you are NOT the intended recipient please contact the sender and dispose
of
this e-mail as soon as possible.
-----Original Message-----
From: Sarah Dysart [mailto:sdysart <@t> mirnarx.com]
Sent: Monday, October 03, 2011 11:55 AM
To: Patsy Ruegg; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] decal question
Formalin? Isn't all decal acid decal?
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
Ruegg
Sent: Monday, October 03, 2011 11:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decal question
Hi Everyone,
I have a new student taking course work at UND and using my lab for her
practical clinical site. She took a test on decal today and there was a
question we didn't know the answer to.
What fixative should not be used for acid decalcification? Would it be
osmium tetroxide?
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
web site <http://www.ihctech.net> www.ihctech.net
This email is confidential and intended solely for the use of the
Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that
is
privileged & confidential within the meaning of applicable law.
Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited.
If
you are NOT the intended recipient please contact the sender and dispose
of
this e-mail as soon as possible.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 16
Date: Tue, 4 Oct 2011 13:17:15 +0100
From: "Edwards, Richard E." <ree3 <@t> leicester.ac.uk>
Subject: RE: [Histonet] decal question
To: 'Jennifer MacDonald' <JMacDonald <@t> mtsac.edu>, Patsy Ruegg
<pruegg <@t> ihctech.net>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>,
"histonet-bounces <@t> lists.utsouthwestern.edu"
<histonet-bounces <@t> lists.utsouthwestern.edu>
Message-ID:
<7722595275A4DD4FA225B92CDBF174A101A4EDF88502 <@t> EXC-MBX3.cfs.le.ac.uk>
Content-Type: text/plain; charset="us-ascii"
Was it decalcifying a tissue fixed in a formalin based fixative using a hydrochloric acid containing decalcifying fluid? as even storage of HCl and formalin in the same area is a bad plan.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald
Sent: 04 October 2011 12:47
To: Patsy Ruegg
Cc: histonet <@t> lists.utsouthwestern.edu; histonet-bounces <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] decal question
Was there more to the question? You would not want to use any fixatives
containing metals, such as mercury, if you will be doing end-point
decalcification determination using x-rays.
"Patsy Ruegg" <pruegg <@t> ihctech.net>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
10/03/2011 11:27 AM
To
"'Sarah Dysart'" <sdysart <@t> mirnarx.com>,
<histonet <@t> lists.utsouthwestern.edu>
cc
Subject
RE: [Histonet] decal question
I think I miss phrased this question, we thought they were asking what
fixative should never be used if you are planning to do acid decal after
fixation? We still think it must be osmium????
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
This email is confidential and intended solely for the use of the
Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that
is
privileged & confidential within the meaning of applicable law.
Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited.
If
you are NOT the intended recipient please contact the sender and dispose
of
this e-mail as soon as possible.
-----Original Message-----
From: Sarah Dysart [mailto:sdysart <@t> mirnarx.com]
Sent: Monday, October 03, 2011 11:55 AM
To: Patsy Ruegg; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] decal question
Formalin? Isn't all decal acid decal?
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
Ruegg
Sent: Monday, October 03, 2011 11:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decal question
Hi Everyone,
I have a new student taking course work at UND and using my lab for her
practical clinical site. She took a test on decal today and there was a
question we didn't know the answer to.
What fixative should not be used for acid decalcification? Would it be
osmium tetroxide?
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
web site <http://www.ihctech.net> www.ihctech.net
This email is confidential and intended solely for the use of the
Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that
is
privileged & confidential within the meaning of applicable law.
Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited.
If
you are NOT the intended recipient please contact the sender and dispose
of
this e-mail as soon as possible.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Tue, 4 Oct 2011 10:36:30 -0400
From: "Sherwood, Margaret" <MSHERWOOD <@t> PARTNERS.ORG>
Subject: Re: [Histonet] Von Kossa Stain for calcium
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A6B <@t> PHSXMB30.partners.org>
Content-Type: text/plain; charset="us-ascii"
We are a research lab and don't do a big volume of special stains. We just
received a project for 35 slides that need Von Kossa Stain.
I acid-washed some small glass staining dishes-no rack required (in the past, we
only had a few requests).
Can I run slides in plastic slide holders (24-place) in an acid-washed beaker
for the silver nitrate step?
Any tips would be appreciated.
Thanks!
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org
The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.
------------------------------
Message: 18
Date: Tue, 4 Oct 2011 10:48:25 -0400
From: rgrow <@t> bmnet.com
Subject: [Histonet] Green counterstain problems
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF30A89D15.B402DD42-ON8525791F.0050C208-8525791F.00515669 <@t> bmnet.com>
Content-Type: text/plain; charset=US-ASCII
Good Morning Histonetters!
I've been having a counterstain problem with my GMS and PAS-F. Both stains
work as they should until the green counterstain is applied. I cannot get
my tissue (control or patient) to uptake the counterstain. I have varied
time, temperature, even companies. I have good controls that have been in
use for years without problems, until now. Only after an extended -10+
minutes,- can I get a blush of color in them. I've even called the slide
company to ask if there has been a change to the charge coating
I'm open to suggestions.
Thanks!
Renee Grow, BA., HT (ASCP)
rgrow <@t> bmnet.com
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN 37804-5016
(865) 977-4744
(865) 977-5766 Fax
________________________________
This communication may contain protected health information (PHI) that is
legally protected from inappropriate disclosure by the Privacy Standards of
the Health Insurance Portability and Accountability Act (HIPAA) and
relevant Tennessee Laws. If you are not the intended recipient, please note
that any dissemination, distribution or copying of this communication is
strictly prohibited. If you have received this message in error, you should
notify the sender immediately by telephone or by return e-mail and delete
this message from your computer. Direct questions to the Blount Memorial
Hospital Privacy Officer at 865-977-4675.
------------------------------
Message: 19
Date: Tue, 4 Oct 2011 10:51:50 -0400
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] RELIA Histology Careers Bulletin Special Edition
for Managers and Supervisors 10-4-2011
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <FF6FFEF1FF3E4934AA326C18A4722E1E <@t> ownerf1abaad51>
Content-Type: text/plain; charset="iso-8859-1"
Hi Histonetters!!
What would make the perfect management position?
Is it the location?
Perhaps the type of lab?
How about the size of the staff/lab?
Maybe it?s the Hours? The Money and/or benefits?
Could it be that a particular situation is the next logical step in your
career?
More than likely the answer is all of the above in different degrees.
That is why I am contacting you with this special bulletin. I am
starting to get a lot of management opportunities and I wanted to touch
base and let you know. Your next opportunity might be just around the
corner and I might have it for you. If you are looking for a position
right now please contact me right away. We can talk about my current
positions OR about a customized search on your behalf. If you aren?t
looking right away but want to let me know what would make a perfect job
for you so that I could keep an eye out, that would be great too. To
do that just shoot me an email at relia1 <@t> earthlink.net or call me toll
free at 866-607-3542.
Here is a list of my current managerial opportunities:
Pathology Lab Manager ? Modesto, CA
Assistant Histology Supervisor ? Austin, TX
Histology Supervisor ? Portland, OR
Histology Manager ? Long Island, NY
All of these clients offer autonomy in your position and histology staff
eager to welcome their new manager.
I really appreciate you taking time out of your busy day to read my
e-mail Thanks Again!
Thank You!
Pam Barker
President
RELIA
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: relia1 <@t> earthlink.net
www.facebook.comPamBarkerRELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia
------------------------------
Message: 20
Date: Tue, 4 Oct 2011 10:56:43 -0400
From: "Bell, Lynne" <Lynne.Bell <@t> cvmc.org>
Subject: RE: [Histonet] Green counterstain problems
To: "'rgrow <@t> bmnet.com'" <rgrow <@t> bmnet.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F9E5562685AF9B498D9D8C1EC996D2440F73465FEA <@t> cvmc-email.CVMC.ORG>
Content-Type: text/plain; charset="us-ascii"
We have had this problem before. Make sure that the glacial acetic acid has been added to the light green stock solution. Cured our problem!!!
Lynne Bell, HT (ASCP)
Histology Team Leader
Central Vermont Medical Center
130 Fisher Road
Berlin, VT 05641
802-371-4923
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of rgrow <@t> bmnet.com
Sent: Tuesday, October 04, 2011 10:48 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Green counterstain problems
Good Morning Histonetters!
I've been having a counterstain problem with my GMS and PAS-F. Both stains
work as they should until the green counterstain is applied. I cannot get
my tissue (control or patient) to uptake the counterstain. I have varied
time, temperature, even companies. I have good controls that have been in
use for years without problems, until now. Only after an extended -10+
minutes,- can I get a blush of color in them. I've even called the slide
company to ask if there has been a change to the charge coating
I'm open to suggestions.
Thanks!
Renee Grow, BA., HT (ASCP)
rgrow <@t> bmnet.com
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN 37804-5016
(865) 977-4744
(865) 977-5766 Fax
________________________________
This communication may contain protected health information (PHI) that is
legally protected from inappropriate disclosure by the Privacy Standards of
the Health Insurance Portability and Accountability Act (HIPAA) and
relevant Tennessee Laws. If you are not the intended recipient, please note
that any dissemination, distribution or copying of this communication is
strictly prohibited. If you have received this message in error, you should
notify the sender immediately by telephone or by return e-mail and delete
this message from your computer. Direct questions to the Blount Memorial
Hospital Privacy Officer at 865-977-4675.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 95, Issue 4
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