[Histonet] decal question

[Patsy Ruegg]

I think I miss phrased this question, we thought they were asking what
fixative should never be used if you are planning to do acid decal after
fixation?  We still think it must be osmium???? 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: Sarah Dysart [mailto:sdysart <@t> mirnarx.com] 
Sent: Monday, October 03, 2011 11:55 AM
To: Patsy Ruegg; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] decal question

Formalin?  Isn't all decal acid decal?

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, October 03, 2011 11:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decal question

Hi Everyone,

 

I have a new student taking course work at UND and using my lab for her
practical clinical site.  She took a test on decal today and there was a
question we didn't know the answer to.

 

What fixative should not be used for acid decalcification?  Would it be
osmium tetroxide?

 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email  <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
web site  <http://www.ihctech.net> www.ihctech.net

 


This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.

 

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