[Histonet] decal question

[Bernice Frederick]

Ann Preece states acid decal uses aqueous solutions od  either formic,nitric or trichloroacetic acid. Other methods mentioned are Ion-exchange resin,electrical ionization and chelation. The histo bible!
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, October 03, 2011 11:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decal question

Hi Everyone,

 

I have a new student taking course work at UND and using my lab for her practical clinical site.  She took a test on decal today and there was a question we didn't know the answer to.

 

What fixative should not be used for acid decalcification?  Would it be osmium tetroxide?

 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email  <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net web site  <http://www.ihctech.net> www.ihctech.net

 


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