From CFarish <@t> csu.edu.au Sat Oct 1 05:57:25 2011 From: CFarish <@t> csu.edu.au (Farish, Craig) Date: Sat Oct 1 05:57:40 2011 Subject: [Histonet] RE: AFB Controls Message-ID: <3232733C82C90D439BF48C9FEF3B7D1C2F78512D98@MAIL01.CSUMain.csu.edu.au> Hi Pamela, try contacting your local large animal vet or a veterinary diagnostic lab. Any case of Ovine or Bovine Johnne's disease would be a great ZN positive control. The bacteria (mycobacterium avium subsp paratuberculosis) is acid fast and congregates in huge numbers in the terminal ileum and ileo-caecal junction (and also the caudal mesenteric lymph node in smaller numbers). About the only problem i've ever come across is that there are so many AFBs it may mask poorly stained section unless you look for individual bacteria in the margins. Hope this helps Craig Craig Farish Senior TO Veterinary Diagnostic Laboratory Charles Sturt University Wagga Wagga NSW, Australia ------------------------------ Message: 12 Date: Thu, 29 Sep 2011 18:23:07 +0000 From: "Marcum, Pamela A" Subject: [Histonet] AFB Controls To: "histonet@lists.utsouthwestern.edu" Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3209697449@Mail2Node2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" Does anyone know where we can get a good AFB control? Ours have exhausted and we can't find a case here with a positive reading. Best Regards, Pamela A Marcum UAMS Slot 502 4301 W Markham Street Little Rock AR 72205 Office: 501-686-7554 Fax: 501-686-7151 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. Charles Sturt University | ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | ONTARIO | ORANGE | SYDNEY | WAGGA WAGGA | Give Generously - Support Young Australians You can help young Australians to go to University and succeed in their studies by giving generously to the Charles Sturt University Foundation. To find out more or to make a donation go to the Foundation web site http://www.csu.edu.au/special/foundation. Australian donations are tax deductible. LEGAL NOTICE This email (and any attachment) is confidential and is intended for the use of the addressee(s) only. If you are not the intended recipient of this email, you must not copy, distribute, take any action in reliance on it or disclose it to anyone. Any confidentiality is not waived or lost by reason of mistaken delivery. Email should be checked for viruses and defects before opening. Charles Sturt University (CSU) does not accept liability for viruses or any consequence which arise as a result of this email transmission. Email communications with CSU may be subject to automated email filtering, which could result in the delay or deletion of a legitimate email before it is read at CSU. The views expressed in this email are not necessarily those of CSU. Charles Sturt University in Australia http://www.csu.edu.au The Chancellery, Panorama Avenue, Bathurst NSW Australia 2795 ABN: 83 878 708 551; CRICOS Provider Numbers: 00005F (NSW), 01947G (VIC), 02960B (ACT) Charles Sturt University in Ontario http://www.charlessturt.ca 860 Harrington Court, Burlington Ontario Canada L7N 3N4 Registration: www.peqab.ca Consider the environment before printing this email. From gmicek <@t> wowway.com Sat Oct 1 19:35:10 2011 From: gmicek <@t> wowway.com (Gmicek) Date: Sat Oct 1 20:14:10 2011 Subject: [Histonet] Re: Histonet Digest, Vol 95, Issue 1 In-Reply-To: <56.2C.01380.AD8478E4@mx01.wow.synacor.com> References: <56.2C.01380.AD8478E4@mx01.wow.synacor.com> Message-ID: <66FD1681-19B3-49F4-B4BD-992893D83558@wowway.com> Make your own with moldy bread. Works great! G Micek Sent from my iPhone On Oct 1, 2011, at 12:07 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Control Tissue (Terry&Star Bullard) > 2. Position Available @ NIH (Bethesda, MD) (Jason Blaine) > 3. Histonet Digest, Vol 94, Issue 40 Nothing down the drain > (Steve McClain) > 4. washing the placenta (An Eerdekens) > 5. Microtome Alignment Tool (Marcia Fisher) > 6. RE: AFB Controls (Farish, Craig) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 30 Sep 2011 13:29:43 -0400 > From: "Terry&Star Bullard" > Subject: [Histonet] Control Tissue > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi all. Does anyone have a resource for control blocks? We are in need > of fungus controls and are trying to save the cost of buying slides. > Thanks all. > > Sheila Haas > Laboratory Supervisor > MicroPath Laboratories, Inc > > > Sheila, > National Society for Histotechnology has a Tissue Control Bank, If you > contact the person below she can help: > > Melinda A Hamilton HT (ASCP) > NSH Tissue Control Bank Curator > Melinda Sokol > > Hope that help! > Star Bullard HT(ASCP) > > > ------------------------------ > > Message: 2 > Date: Fri, 30 Sep 2011 17:33:27 +0000 > From: Jason Blaine > Subject: [Histonet] Position Available @ NIH (Bethesda, MD) > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Seeking highly skilled scientist or histologist who is well versed in IHC. This position is in the National Institute of Mental Health (NIMH) and thus requires prior neuroscience and non-human primate experience. Please note that we are NOT necessarily seeking ASCP histology certificated individuals and that prior related experience will be preferred over certification. > > The position will support research in the NIMH. The position will provide histology support service for the complete laboratory (has 5 sections within it). This person will be the go to person for histology and IHC within the lab and its 5 sections. This individual will be expected to function as a resource to all of the lab's not solely as a technician but rather as a histology/IHC expert to advise the teams with their histology/IHC needs. This will be a collaborative research effort and will not offer personal independent research. Non-human primate model is the principal concern. Experience with IHC, Fluoro-ruby and green, D2, BrDu, tyrosine hyroxylase, perfusion, brain removal and prep, and much more is desired. > > If this opportunity is not right for you I ask that you point me in the direction of someone who may be appropriately qualified - maybe a colleague of yours, or former boss, or collaborator. The opportunity is a fulltime, salaried, indefinite, contractor position with benefits. > > Qualified candidates please submit a current CV/resume (in MS Word format) to jblaine@astrixinc.com. > > Thanks - > Jason Blaine > jblaine@astrixinc.com > > > > ------------------------------ > > Message: 3 > Date: Fri, 30 Sep 2011 14:08:27 -0400 > From: "Steve McClain" > Subject: [Histonet] Histonet Digest, Vol 94, Issue 40 Nothing down the > drain > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Yes nothing down the drain (except maybe the lithium carbonate bluing). > > Hematoxylin goes into medical waste service. > > Used Eosin gets reused a 10-20 ml at a time into the first absolute on > the processors as a marker of carryover (as the later alcohols turn red, > we have a visual indicator of the need to change, also helps with > embedding since collagen stains more than epithelium/epidermis). > > Alcohols after the de-waxing xylenes and from the processor purges go to > the medical waste service. > > Other alcohols get recycled. > > All the Xylenes get recycled. > > Liquid Waste from the recycler goes to medical waste service. > > Paraffins go into the trash. > > > > > > Steve > > Steve A. McClain, MD > > McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 > > > > > > ------------------------------ > > Message: 4 > Date: Fri, 30 Sep 2011 21:18:10 +0200 > From: An Eerdekens > Subject: [Histonet] washing the placenta > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <09F2BA9A1E06C64D9F090FF0FD0D73179763AFDBC7@EX2007-MBX-2.uz.kuleuven.ac.be> > > Content-Type: text/plain; charset="us-ascii" > > Dear all, > > Is there someone with experience in washing of placentae before sampling? > > Regards > > Dr An Eerdekens > > > ------------------------------ > > Message: 5 > Date: Fri, 30 Sep 2011 19:33:59 +0000 > From: Marcia Fisher > Subject: [Histonet] Microtome Alignment Tool > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > We just purchased one from American Master Tech and really like the results. We now have added this task to our monthly maintenance sheets. > > Marcia Fisher > Histology Supervisor/Lab Safety Officer > El Centro Regional Medical Center > > > ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. > > > > > > ------------------------------ > > Message: 6 > Date: Sat, 1 Oct 2011 20:57:25 +1000 > From: "Farish, Craig" > Subject: [Histonet] RE: AFB Controls > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <3232733C82C90D439BF48C9FEF3B7D1C2F78512D98@MAIL01.CSUMain.csu.edu.au> > Content-Type: text/plain; charset="us-ascii" > > Hi Pamela, try contacting your local large animal vet or a veterinary diagnostic lab. Any case of Ovine or Bovine Johnne's disease would be a great ZN positive control. The bacteria (mycobacterium avium subsp paratuberculosis) is acid fast and congregates in huge numbers in the terminal ileum and ileo-caecal junction (and also the caudal mesenteric lymph node in smaller numbers). > About the only problem i've ever come across is that there are so many AFBs it may mask poorly stained section unless you look for individual bacteria in the margins. > Hope this helps > Craig > > Craig Farish > Senior TO > Veterinary Diagnostic Laboratory > Charles Sturt University > Wagga Wagga > NSW, Australia > > > ------------------------------ > > Message: 12 > Date: Thu, 29 Sep 2011 18:23:07 +0000 > From: "Marcum, Pamela A" > Subject: [Histonet] AFB Controls > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <41D3A1AF6FEF0643BDC89E0516A6EA3209697449@Mail2Node2.ad.uams.edu> > Content-Type: text/plain; charset="us-ascii" > > Does anyone know where we can get a good AFB control? Ours have exhausted and we can't find a case here with a positive reading. > > Best Regards, > > Pamela A Marcum > UAMS > Slot 502 > 4301 W Markham Street > Little Rock AR 72205 > Office: 501-686-7554 > Fax: 501-686-7151 > > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, > use, disclosure or distribution is prohibited. If you are not the > intended recipient, please contact the sender by reply > e-mail and destroy all copies of the original message.. > Charles Sturt University > > | ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | ONTARIO | ORANGE | SYDNEY | WAGGA WAGGA | > > Give Generously - Support Young Australians > > You can help young Australians to go to University and succeed in their studies by giving generously to the Charles Sturt University Foundation. To find out more or to make a donation go to the Foundation web site http://www.csu.edu.au/special/foundation. Australian donations are tax deductible. > > LEGAL NOTICE > This email (and any attachment) is confidential and is intended for the use of the addressee(s) only. If you are not the intended recipient of this email, you must not copy, distribute, take any action in reliance on it or disclose it to anyone. Any confidentiality is not waived or lost by reason of mistaken delivery. Email should be checked for viruses and defects before opening. Charles Sturt University (CSU) does not accept liability for viruses or any consequence which arise as a result of this email transmission. Email communications with CSU may be subject to automated email filtering, which could result in the delay or deletion of a legitimate email before it is read at CSU. The views expressed in this email are not necessarily those of CSU. > > Charles Sturt University in Australia http://www.csu.edu.au The Chancellery, Panorama Avenue, Bathurst NSW Australia 2795 ABN: 83 878 708 551; CRICOS Provider Numbers: 00005F (NSW), 01947G (VIC), 02960B (ACT) > > Charles Sturt University in Ontario http://www.charlessturt.ca 860 Harrington Court, Burlington Ontario Canada L7N 3N4 Registration: www.peqab.ca > > Consider the environment before printing this email. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 95, Issue 1 > *************************************** From LBUSTAMANTE <@t> cvm.tamu.edu Sun Oct 2 20:10:31 2011 From: LBUSTAMANTE <@t> cvm.tamu.edu (Lin Bustamante) Date: Sun Oct 2 20:10:35 2011 Subject: [Histonet] CLIA Inspection Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC903EAF879@CVMMB02.cvm.tamu.edu> Does anyone know were I could find the CLIA regulations for inspecting a small lab? I have reviewed the CLIA web site and I cannot find what I am looking for. Thank you. Lin Bustamante From mjdessoye <@t> wvhcs.org Mon Oct 3 08:50:26 2011 From: mjdessoye <@t> wvhcs.org (Dessoye, Michael J) Date: Mon Oct 3 08:50:36 2011 Subject: [Histonet] Slide/Block Retention Message-ID: Hello Histonet, What policy is everyone following for slide and block retention? We are not CAP, however we currently keep slides and blocks for 10 years. My director wants to decrease that period to 2 years, which is the Joint Commission standard. I would like to keep 10 years because we frequently are asked to send slides for consult and review that are several years old. It is also helpful when looking for hard-to-find control tissue. However these reasons are apparently not sufficient. I'm leaning towards keeping the 10 year policy, but I need additional justification. Or does anyone think the 2 year period is sufficient? Thanks, Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From rjbuesa <@t> yahoo.com Mon Oct 3 09:00:51 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 3 09:00:55 2011 Subject: [Histonet] Slide/Block Retention In-Reply-To: Message-ID: <1317650451.30047.YahooMailClassic@web65715.mail.ac4.yahoo.com> We keep our blocks 9 years. Ren? J. --- On Mon, 10/3/11, Dessoye, Michael J wrote: From: Dessoye, Michael J Subject: [Histonet] Slide/Block Retention To: histonet@lists.utsouthwestern.edu Date: Monday, October 3, 2011, 9:50 AM Hello Histonet, What policy is everyone following for slide and block retention?? We are not CAP, however we currently keep slides and blocks for 10 years.? My director wants to decrease that period to 2 years, which is the Joint Commission standard.? I would like to keep 10 years because we frequently are asked to send slides for consult and review that are several years old.? It is also helpful when looking for hard-to-find control tissue.? However these reasons are apparently not sufficient.? I'm leaning towards keeping the 10 year policy, but I need additional justification.? Or does anyone think the 2 year period is sufficient? Thanks, Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org ? | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Mon Oct 3 09:07:07 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Oct 3 09:07:16 2011 Subject: [Histonet] Slide/Block Retention In-Reply-To: <1317650451.30047.YahooMailClassic@web65715.mail.ac4.yahoo.com> References: <1317650451.30047.YahooMailClassic@web65715.mail.ac4.yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640827B748DA@CHEXCMS10.one.ads.che.org> In today's world with all the molecular test advances, I'd keep malignancies as long as forever. Your state should have guidelines. I would look there. GA is the same as CAP - 10 years. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, October 03, 2011 10:01 To: histonet@lists.utsouthwestern.edu; Michael JDessoye Subject: Re: [Histonet] Slide/Block Retention We keep our blocks 9 years. Ren? J. --- On Mon, 10/3/11, Dessoye, Michael J wrote: From: Dessoye, Michael J Subject: [Histonet] Slide/Block Retention To: histonet@lists.utsouthwestern.edu Date: Monday, October 3, 2011, 9:50 AM Hello Histonet, What policy is everyone following for slide and block retention?? We are not CAP, however we currently keep slides and blocks for 10 years.? My director wants to decrease that period to 2 years, which is the Joint Commission standard.? I would like to keep 10 years because we frequently are asked to send slides for consult and review that are several years old.? It is also helpful when looking for hard-to-find control tissue.? However these reasons are apparently not sufficient.? I'm leaning towards keeping the 10 year policy, but I need additional justification.? Or does anyone think the 2 year period is sufficient? Thanks, Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org ? | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From JThawley <@t> ShoreMemorial.org Mon Oct 3 09:06:35 2011 From: JThawley <@t> ShoreMemorial.org (JThawley@ShoreMemorial.org) Date: Mon Oct 3 09:07:54 2011 Subject: [Histonet] Slide/Block Retention In-Reply-To: Message-ID: Hey Mike, You should look into your state regulations, sometimes they are a little different. We keep all our slides/blocks for at least 10 years. Jennifer Thawley HT, ASCP Histology Supervisor Shore Memorial Hospital (609) 653-3940 "Dessoye, Michael J" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Slide/Block Retention 10/03/2011 09:50 AM Hello Histonet, What policy is everyone following for slide and block retention? We are not CAP, however we currently keep slides and blocks for 10 years. My director wants to decrease that period to 2 years, which is the Joint Commission standard. I would like to keep 10 years because we frequently are asked to send slides for consult and review that are several years old. It is also helpful when looking for hard-to-find control tissue. However these reasons are apparently not sufficient. I'm leaning towards keeping the 10 year policy, but I need additional justification. Or does anyone think the 2 year period is sufficient? Thanks, Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt <@t> uphs.upenn.edu Mon Oct 3 09:15:08 2011 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Mon Oct 3 09:14:58 2011 Subject: [Histonet] Slide/Block Retention In-Reply-To: References: Message-ID: <91FD001DCC6DC84EA6A4B12BB93136789C2FE5@uphmasphi007.UPHS.PENNHEALTH.PRV> Wow, that is very unusual that the director suggests 2 years, normally the medical directors fault to saving everything! :) 10 years is definitely the requirement by TJC and CAP to my knowledge. If I come across some supporting documentation, I will forward it on but I am positive that 2 years will definitely not constitute compliance. Caroline M. Pratt Penn Medicine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Monday, October 03, 2011 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide/Block Retention Hello Histonet, What policy is everyone following for slide and block retention? We are not CAP, however we currently keep slides and blocks for 10 years. My director wants to decrease that period to 2 years, which is the Joint Commission standard. I would like to keep 10 years because we frequently are asked to send slides for consult and review that are several years old. It is also helpful when looking for hard-to-find control tissue. However these reasons are apparently not sufficient. I'm leaning towards keeping the 10 year policy, but I need additional justification. Or does anyone think the 2 year period is sufficient? Thanks, Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From mamawooo <@t> hotmail.com Mon Oct 3 09:23:20 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Mon Oct 3 09:23:24 2011 Subject: [Histonet] Slide/Block Retention In-Reply-To: <1317650451.30047.YahooMailClassic@web65715.mail.ac4.yahoo.com> References: , <1317650451.30047.YahooMailClassic@web65715.mail.ac4.yahoo.com> Message-ID: CAP standard is 10 years for tissue blocks. I would caution not keeping them for that period for several reasons. One is that it may put you in a bad position regarding legal cases. You could be asked why you don't follow the most strict standard and be expected to explain why. The second is for subsequent patient care. When Her-2 first come on the scene we were pulling blocks from cases older than 2 years old. You never know what new prognostic/predictive markers will appear on the scene. If you don't have the original tumor material you may be doing your patients a disservice. If I had blocks at your institution I would want to keep them myself rather than have them destroyed.Jan M.Omaha > Date: Mon, 3 Oct 2011 07:00:51 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; mjdessoye@wvhcs.org > Subject: Re: [Histonet] Slide/Block Retention > CC: > > We keep our blocks 9 years. > Ren? J. > > --- On Mon, 10/3/11, Dessoye, Michael J wrote: > > > From: Dessoye, Michael J > Subject: [Histonet] Slide/Block Retention > To: histonet@lists.utsouthwestern.edu > Date: Monday, October 3, 2011, 9:50 AM > > > Hello Histonet, > > What policy is everyone following for slide and block retention? We are > not CAP, however we currently keep slides and blocks for 10 years. My > director wants to decrease that period to 2 years, which is the Joint > Commission standard. I would like to keep 10 years because we > frequently are asked to send slides for consult and review that are > several years old. It is also helpful when looking for hard-to-find > control tissue. However these reasons are apparently not sufficient. > > I'm leaning towards keeping the 10 year policy, but I need additional > justification. Or does anyone think the 2 year period is sufficient? > > Thanks, > Mike > > Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health > Care System | mjdessoye@wvhcs.org | > 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: > 570-552-1526 > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Wyoming Valley Health Care System. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Mon Oct 3 09:31:50 2011 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Mon Oct 3 09:32:07 2011 Subject: [Histonet] BAP1 from Santa Cruz Biotech Message-ID: <24B7B291CC88D04AB663958E77A1F59DDD87@ex09.net.ucsf.edu> Hello all, Does anyone have a working non-Ventana protocol for this antibody? We need Dako. We got it working at 1:100, HIER in Dako pH9, antibody incubate 30 min, Envision Dual Link 30 min but the researcher who wants it says that it produces uneven staining on the test tissue. She wants it to work like the BAP1 at another facility who got it to work (intermittently only) on one of 2 Ventana instruments. Thanks in advance for your help! Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 From MSHERWOOD <@t> PARTNERS.ORG Mon Oct 3 10:09:36 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Mon Oct 3 10:09:58 2011 Subject: [Histonet] Slide/Block Retention In-Reply-To: References: , <1317650451.30047.YahooMailClassic@web65715.mail.ac4.yahoo.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A60@PHSXMB30.partners.org> We are a research path lab, but we follow the same guidelines. We keep 2 years in the lab and 8 years in off-site storage. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Monday, October 03, 2011 10:23 AM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; mjdessoye@wvhcs.org Subject: RE: [Histonet] Slide/Block Retention CAP standard is 10 years for tissue blocks. I would caution not keeping them for that period for several reasons. One is that it may put you in a bad position regarding legal cases. You could be asked why you don't follow the most strict standard and be expected to explain why. The second is for subsequent patient care. When Her-2 first come on the scene we were pulling blocks from cases older than 2 years old. You never know what new prognostic/predictive markers will appear on the scene. If you don't have the original tumor material you may be doing your patients a disservice. If I had blocks at your institution I would want to keep them myself rather than have them destroyed.Jan M.Omaha > Date: Mon, 3 Oct 2011 07:00:51 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; mjdessoye@wvhcs.org > Subject: Re: [Histonet] Slide/Block Retention > CC: > > We keep our blocks 9 years. > Ren? J. > > --- On Mon, 10/3/11, Dessoye, Michael J wrote: > > > From: Dessoye, Michael J > Subject: [Histonet] Slide/Block Retention > To: histonet@lists.utsouthwestern.edu > Date: Monday, October 3, 2011, 9:50 AM > > > Hello Histonet, > > What policy is everyone following for slide and block retention? We are > not CAP, however we currently keep slides and blocks for 10 years. My > director wants to decrease that period to 2 years, which is the Joint > Commission standard. I would like to keep 10 years because we > frequently are asked to send slides for consult and review that are > several years old. It is also helpful when looking for hard-to-find > control tissue. However these reasons are apparently not sufficient. > > I'm leaning towards keeping the 10 year policy, but I need additional > justification. Or does anyone think the 2 year period is sufficient? > > Thanks, > Mike > > Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health > Care System | mjdessoye@wvhcs.org | > 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: > 570-552-1526 > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Wyoming Valley Health Care System. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From julie.hinsinger <@t> umontreal.ca Mon Oct 3 10:40:55 2011 From: julie.hinsinger <@t> umontreal.ca (Hinsinger Julie) Date: Mon Oct 3 10:41:08 2011 Subject: [Histonet] Atlas of laboratory mouse histology Message-ID: <070FB8DB1EFA214292C54DCBECEB27CF02566A13@MAPIUDEM2.sim.umontreal.ca> Does anyone know of an atlas of mouse histology? Thanks for your comments Julie Hinsinger Histology Facility Manager IRIC - Pav. M. Coutu - Local 3440 2950, chemin polytechnique - Montr?al (Qc) H3T 1J4 From pruegg <@t> ihctech.net Mon Oct 3 11:32:08 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Oct 3 11:32:14 2011 Subject: [Histonet] decal question Message-ID: <3F1C5E41D5E34F239114D9B03324BCD0@Patsyoffice> Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From tpodawiltz <@t> lrgh.org Mon Oct 3 11:40:14 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Oct 3 11:40:25 2011 Subject: [Histonet] Slide/Block Retention In-Reply-To: References: , <1317650451.30047.YahooMailClassic@web65715.mail.ac4.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DF2EA80E@LRGHEXVS1.practice.lrgh.org> We are state inspected under CLIA, which block retention is two years, however we follow the more stricter CAP of ten years. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Monday, October 03, 2011 10:23 AM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; mjdessoye@wvhcs.org Subject: RE: [Histonet] Slide/Block Retention CAP standard is 10 years for tissue blocks. I would caution not keeping them for that period for several reasons. One is that it may put you in a bad position regarding legal cases. You could be asked why you don't follow the most strict standard and be expected to explain why. The second is for subsequent patient care. When Her-2 first come on the scene we were pulling blocks from cases older than 2 years old. You never know what new prognostic/predictive markers will appear on the scene. If you don't have the original tumor material you may be doing your patients a disservice. If I had blocks at your institution I would want to keep them myself rather than have them destroyed.Jan M.Omaha > Date: Mon, 3 Oct 2011 07:00:51 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; mjdessoye@wvhcs.org > Subject: Re: [Histonet] Slide/Block Retention > CC: > > We keep our blocks 9 years. > Ren? J. > > --- On Mon, 10/3/11, Dessoye, Michael J wrote: > > > From: Dessoye, Michael J > Subject: [Histonet] Slide/Block Retention > To: histonet@lists.utsouthwestern.edu > Date: Monday, October 3, 2011, 9:50 AM > > > Hello Histonet, > > What policy is everyone following for slide and block retention? We are > not CAP, however we currently keep slides and blocks for 10 years. My > director wants to decrease that period to 2 years, which is the Joint > Commission standard. I would like to keep 10 years because we > frequently are asked to send slides for consult and review that are > several years old. It is also helpful when looking for hard-to-find > control tissue. However these reasons are apparently not sufficient. > > I'm leaning towards keeping the 10 year policy, but I need additional > justification. Or does anyone think the 2 year period is sufficient? > > Thanks, > Mike > > Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health > Care System | mjdessoye@wvhcs.org | > 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: > 570-552-1526 > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Wyoming Valley Health Care System. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From TMcNemar <@t> lmhealth.org Mon Oct 3 11:50:34 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Oct 3 11:50:38 2011 Subject: [Histonet] RE: Slide/Block Retention In-Reply-To: References: Message-ID: Are you including Cytologies as well? Cytology has the requirement of a five year look-back. We keep all surgical and cytology blocks and slides for 10 years. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Monday, October 03, 2011 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide/Block Retention Hello Histonet, What policy is everyone following for slide and block retention? We are not CAP, however we currently keep slides and blocks for 10 years. My director wants to decrease that period to 2 years, which is the Joint Commission standard. I would like to keep 10 years because we frequently are asked to send slides for consult and review that are several years old. It is also helpful when looking for hard-to-find control tissue. However these reasons are apparently not sufficient. I'm leaning towards keeping the 10 year policy, but I need additional justification. Or does anyone think the 2 year period is sufficient? Thanks, Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Rcartun <@t> harthosp.org Mon Oct 3 12:20:42 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Oct 3 12:20:57 2011 Subject: [Histonet] Slide/Block Retention In-Reply-To: References: Message-ID: <4E89B6AA.7400.0077.1@harthosp.org> We currently keep slides and paraffin blocks (surgical pathology) for 17 years; I would keep more, but laboratory administration keeps tell me to discard because we don't have sufficient storage space. It's not unusual for me to receive a request for a block(s) from the 1990's for IHC, molecular, or genetic testing especially now that we have entered the arena for "Personalized Medicine". Also, I find myself re-testing specimens from the 1990's and 2000's with new generation antibodies, probes, and detection systems. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Dessoye, Michael J" 10/3/2011 9:50 AM >>> Hello Histonet, What policy is everyone following for slide and block retention? We are not CAP, however we currently keep slides and blocks for 10 years. My director wants to decrease that period to 2 years, which is the Joint Commission standard. I would like to keep 10 years because we frequently are asked to send slides for consult and review that are several years old. It is also helpful when looking for hard-to-find control tissue. However these reasons are apparently not sufficient. I'm leaning towards keeping the 10 year policy, but I need additional justification. Or does anyone think the 2 year period is sufficient? Thanks, Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abuchiane <@t> bmhvt.org Mon Oct 3 12:24:16 2011 From: abuchiane <@t> bmhvt.org (Anita Buchiane) Date: Mon Oct 3 12:24:21 2011 Subject: [Histonet] Cpt Charge Message-ID: <4034E71604330C4B8E10D1538DFB2455CD38@BMHEXCH02.bmhvt.org> Can we still only bill 88342 per antibody /per part or has that been changed? For instance: If we do a Calponin and an Actin on 3 blocks from one part do we charge 88342 x 2 or x 6? I thought I remember reading that it was changed to allow for charging per slide but I can not find the reference. Thanks _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ From julie.hinsinger <@t> umontreal.ca Mon Oct 3 12:27:52 2011 From: julie.hinsinger <@t> umontreal.ca (Hinsinger Julie) Date: Mon Oct 3 12:28:29 2011 Subject: [Histonet] RE: Atlas of laboratory mouse histology Message-ID: <070FB8DB1EFA214292C54DCBECEB27CF02566A8F@MAPIUDEM2.sim.umontreal.ca> Just to let you know about the first answers: - There is an online atlas that was brought to my attention : http://www.mouseatlas.org/data/mouse/libraries/SM202 - And most referred book was Atlas of Mouse Development by Matthew H. Kaufman with a revised edition, printed in 2002. (Good for mouse embryo stages) I might have another question: As for adult organs, is there a general histology atlas you refer to? In our lab, we use "Pathology of the Mouse" Robert R. Maronpot As we work on both human and mouse tissues, I have found a new book that might be interesting "Comparative Anatomy and Histology: A Mouse and Human Atlas" Have you heard about it? It will be released in December 2011. http://www.amazon.ca/Comparative-Anatomy-Histology-Expert-Consult/dp/0123813611/ref=sr_1_1?s=books&ie=UTF8&qid=1317661829&sr=1-1 It aims at the new mouse investigator as well as medical and veterinary pathologists who need to expand their knowledge base into comparative anatomy and histology. It guides the reader through normal mouse anatomy and histology using direct comparison to the human. The side by side comparison of mouse and human tissues highlight the unique biology of the mouse, which has great impact on the validation of mouse models of human disease. Seems great! I don't know... Julie De : Hinsinger Julie Envoy? : 3 octobre 2011 11:41 ? : 'histonet@lists.utsouthwestern.edu' Objet : Atlas of laboratory mouse histology Does anyone know of an atlas of mouse histology? Thanks for your comments Julie Hinsinger Histology Facility Manager IRIC - Pav. M. Coutu - Local 3440 2950, chemin polytechnique - Montr?al (Qc) H3T 1J4 From JWeems <@t> sjha.org Mon Oct 3 12:54:23 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Oct 3 12:54:28 2011 Subject: [Histonet] RE: Cpt Charge In-Reply-To: <4034E71604330C4B8E10D1538DFB2455CD38@BMHEXCH02.bmhvt.org> References: <4034E71604330C4B8E10D1538DFB2455CD38@BMHEXCH02.bmhvt.org> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640827B749CC@CHEXCMS10.one.ads.che.org> You can now charge per block. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anita Buchiane Sent: Monday, October 03, 2011 13:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cpt Charge Can we still only bill 88342 per antibody /per part or has that been changed? For instance: If we do a Calponin and an Actin on 3 blocks from one part do we charge 88342 x 2 or x 6? I thought I remember reading that it was changed to allow for charging per slide but I can not find the reference. Thanks _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From sdysart <@t> mirnarx.com Mon Oct 3 12:55:04 2011 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Mon Oct 3 12:55:12 2011 Subject: [Histonet] decal question In-Reply-To: <3F1C5E41D5E34F239114D9B03324BCD0@Patsyoffice> References: <3F1C5E41D5E34F239114D9B03324BCD0@Patsyoffice> Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5079FA@SN2PRD0702MB110.namprd07.prod.outlook.com> Formalin? Isn't all decal acid decal? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSetlak <@t> childrensmemorial.org Mon Oct 3 13:01:04 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Mon Oct 3 13:01:12 2011 Subject: [Histonet] RE: Cpt Charge In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640827B749CC@CHEXCMS10.one.ads.che.org> References: <4034E71604330C4B8E10D1538DFB2455CD38@BMHEXCH02.bmhvt.org> <92AD9B20A6C38C4587A9FEBE3A30E1640827B749CC@CHEXCMS10.one.ads.che.org> Message-ID: <7111DB39D045004C9CF29E79C71B28BC1093D4D479@CMHEXCC01MBX.childrensmemorial.org> Where we can find a reference to this.... I know I'll get questions when I change it? Thanks, Lisa Children's Memorial Hospital Chicago, Il -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, October 03, 2011 12:54 PM To: Anita Buchiane; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cpt Charge You can now charge per block. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anita Buchiane Sent: Monday, October 03, 2011 13:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cpt Charge Can we still only bill 88342 per antibody /per part or has that been changed? For instance: If we do a Calponin and an Actin on 3 blocks from one part do we charge 88342 x 2 or x 6? I thought I remember reading that it was changed to allow for charging per slide but I can not find the reference. Thanks _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon Oct 3 13:17:59 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Oct 3 13:18:36 2011 Subject: [Histonet] decal question In-Reply-To: <3F1C5E41D5E34F239114D9B03324BCD0@Patsyoffice> References: <3F1C5E41D5E34F239114D9B03324BCD0@Patsyoffice> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E17A1B4@evcspmbx3.ads.northwestern.edu> Ann Preece states acid decal uses aqueous solutions od either formic,nitric or trichloroacetic acid. Other methods mentioned are Ion-exchange resin,electrical ionization and chelation. The histo bible! Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Mon Oct 3 13:25:32 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Oct 3 13:25:42 2011 Subject: [Histonet] decal question Message-ID: Alcoholic formalin or 70% ethanol as, quoting Gayle Callis, "alcohol can slow or prevent decalcification" Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From pruegg <@t> ihctech.net Mon Oct 3 13:25:52 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Oct 3 13:25:55 2011 Subject: [Histonet] decal question In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5079FA@SN2PRD0702MB110.namprd07.prod.outlook.com> References: <3F1C5E41D5E34F239114D9B03324BCD0@Patsyoffice> <8A70A9B2ECDD084DACFE6C59FCF86D5079FA@SN2PRD0702MB110.namprd07.prod.outlook.com> Message-ID: <7104CA2AA7E64D859B3B1084AD323E5B@Patsyoffice> I think I miss phrased this question, we thought they were asking what fixative should never be used if you are planning to do acid decal after fixation? We still think it must be osmium???? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Sarah Dysart [mailto:sdysart@mirnarx.com] Sent: Monday, October 03, 2011 11:55 AM To: Patsy Ruegg; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decal question Formalin? Isn't all decal acid decal? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Mon Oct 3 13:30:26 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon Oct 3 13:30:30 2011 Subject: [Histonet] Re: Slide/Block Retention Message-ID: I don't care what the herrn inshpektors say, the fact is that patient care - with the rapid growth of genetic and other molecular techniques - I think 10 years is the minimum for retaining paraffin blocks, and I'd be a lot more comfortable with 20. In the future I expect we'll be retrieving blocks to do studies on members of patients' families. And if your kid flunks out of college, I reckon you can sue the OB that delivered him - so that's >20 years for placentas. An issue rarely mentioned: when a tissue lab closes, everything is often discarded. We need requirements for tissue retention when labs close. I have no idea how this could be done. But as regulations increase and our economy declines, there'll be a lot of tissue labs closing in the next decade. Bob Richmond Samurai Pathologist Knoxville TN From amario3 <@t> uic.edu Mon Oct 3 14:34:07 2011 From: amario3 <@t> uic.edu (Andrea Marion) Date: Mon Oct 3 14:34:10 2011 Subject: [Histonet] RE: Atlas of laboratory mouse histology Message-ID: <0c00e9909cf1a85757b0f687b639347f.squirrel@webmail.uic.edu> Hi Julie - Other than the ones you already found, I have two (okay 3) more recommendations: 1- Theiler's classic The House Mouse: Atlas of Embryonic Development has a great histology of mouse embryos. I use it side-by-side with the Kaufmann text for embryo histology. It's freely available for download, which is very handy: http://www.emouseatlas.org/Atlas/Theiler_book_download.html 2- There is a quick online guide for adult mouse histology at http://ctrgenpath.net/static/atlas/mousehistology/Windows/introduction.html It's a nice page for quickly checking something, but there is not a lot of in depth coverage. 3 - I also suggest searching on the mouse research listserv, I've seen it discussed there before and a quick search turned up a number of recommendations: http://www.informatics.jax.org/lyris-cgi/lyris.pl?visit=mgi-list Good luck, Andrea Andrea Marion Graduate Student University of Illinois at Chicago [Histonet] RE: Atlas of laboratory mouse histology Hinsinger Julie julie.hinsinger <@t> umontreal.ca Mon Oct 3 12:27:52 CDT 2011 Previous message: [Histonet] RE: Cpt Charge Next message: [Histonet] decal question Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] Just to let you know about the first answers: - There is an online atlas that was brought to my attention : http://www.mouseatlas.org/data/mouse/libraries/SM202 - And most referred book was Atlas of Mouse Development by Matthew H. Kaufman with a revised edition, printed in 2002. (Good for mouse embryo stages) I might have another question: As for adult organs, is there a general histology atlas you refer to? In our lab, we use "Pathology of the Mouse" Robert R. Maronpot As we work on both human and mouse tissues, I have found a new book that might be interesting "Comparative Anatomy and Histology: A Mouse and Human Atlas" Have you heard about it? It will be released in December 2011. http://www.amazon.ca/Comparative-Anatomy-Histology-Expert-Consult/dp/0123813611/ref=sr_1_1?s=books&ie=UTF8&qid=1317661829&sr=1-1 It aims at the new mouse investigator as well as medical and veterinary pathologists who need to expand their knowledge base into comparative anatomy and histology. It guides the reader through normal mouse anatomy and histology using direct comparison to the human. The side by side comparison of mouse and human tissues highlight the unique biology of the mouse, which has great impact on the validation of mouse models of human disease. Seems great! I don't know... Julie De : Hinsinger Julie Envoy? : 3 octobre 2011 11:41 ? : 'histonet <@t> lists.utsouthwestern.edu' Objet : Atlas of laboratory mouse histology Does anyone know of an atlas of mouse histology? Thanks for your comments Julie Hinsinger Histology Facility Manager IRIC - Pav. M. Coutu - Local 3440 2950, chemin polytechnique - Montr?al (Qc) H3T 1J4 From lblazek <@t> digestivespecialists.com Mon Oct 3 14:50:15 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Oct 3 14:50:27 2011 Subject: [Histonet] Re: Slide/Block Retention In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39127401B36E@IBMB7Exchange.digestivespecialists.com> There is an inspection question that addresses what happens to material if a lab closes. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Monday, October 03, 2011 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Slide/Block Retention I don't care what the herrn inshpektors say, the fact is that patient care - with the rapid growth of genetic and other molecular techniques - I think 10 years is the minimum for retaining paraffin blocks, and I'd be a lot more comfortable with 20. In the future I expect we'll be retrieving blocks to do studies on members of patients' families. And if your kid flunks out of college, I reckon you can sue the OB that delivered him - so that's >20 years for placentas. An issue rarely mentioned: when a tissue lab closes, everything is often discarded. We need requirements for tissue retention when labs close. I have no idea how this could be done. But as regulations increase and our economy declines, there'll be a lot of tissue labs closing in the next decade. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FUNKM <@t> mercyhealth.com Mon Oct 3 15:20:34 2011 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Mon Oct 3 15:20:45 2011 Subject: [Histonet] HP controls Message-ID: <4E89D2C2.E948.00AC.1@mercyhealth.com> We are having difficult with the HP control for IHC staining - If you are purchasing controls or if you have controls that you might share I would really appreciate your help. Thanks so much -Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 From amosbrooks <@t> gmail.com Mon Oct 3 16:27:28 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Oct 3 16:27:33 2011 Subject: [Histonet] Atlas of laboratory mouse histology Message-ID: Hi, I would be interested in this reference as well. Please post to the group if you have any suggestions. Amos On Mon, Oct 3, 2011 at 1:00 PM, wrote: > Message: 10 > Date: Mon, 3 Oct 2011 11:40:55 -0400 > From: "Hinsinger Julie" > Subject: [Histonet] Atlas of laboratory mouse histology > To: > Message-ID: > < > 070FB8DB1EFA214292C54DCBECEB27CF02566A13@MAPIUDEM2.sim.umontreal.ca> > Content-Type: text/plain; charset="iso-8859-1" > > Does anyone know of an atlas of mouse histology? > > Thanks for your comments > > > > Julie Hinsinger > > Histology Facility Manager > From JMacDonald <@t> mtsac.edu Tue Oct 4 06:46:50 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Oct 4 06:46:57 2011 Subject: [Histonet] decal question In-Reply-To: <7104CA2AA7E64D859B3B1084AD323E5B@Patsyoffice> Message-ID: Was there more to the question? You would not want to use any fixatives containing metals, such as mercury, if you will be doing end-point decalcification determination using x-rays. "Patsy Ruegg" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/03/2011 11:27 AM To "'Sarah Dysart'" , cc Subject RE: [Histonet] decal question I think I miss phrased this question, we thought they were asking what fixative should never be used if you are planning to do acid decal after fixation? We still think it must be osmium???? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Sarah Dysart [mailto:sdysart@mirnarx.com] Sent: Monday, October 03, 2011 11:55 AM To: Patsy Ruegg; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decal question Formalin? Isn't all decal acid decal? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue Oct 4 07:17:15 2011 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Tue Oct 4 07:17:34 2011 Subject: [Histonet] decal question In-Reply-To: References: <7104CA2AA7E64D859B3B1084AD323E5B@Patsyoffice> Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4EDF88502@EXC-MBX3.cfs.le.ac.uk> Was it decalcifying a tissue fixed in a formalin based fixative using a hydrochloric acid containing decalcifying fluid? as even storage of HCl and formalin in the same area is a bad plan. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: 04 October 2011 12:47 To: Patsy Ruegg Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] decal question Was there more to the question? You would not want to use any fixatives containing metals, such as mercury, if you will be doing end-point decalcification determination using x-rays. "Patsy Ruegg" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/03/2011 11:27 AM To "'Sarah Dysart'" , cc Subject RE: [Histonet] decal question I think I miss phrased this question, we thought they were asking what fixative should never be used if you are planning to do acid decal after fixation? We still think it must be osmium???? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Sarah Dysart [mailto:sdysart@mirnarx.com] Sent: Monday, October 03, 2011 11:55 AM To: Patsy Ruegg; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decal question Formalin? Isn't all decal acid decal? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Tue Oct 4 09:36:30 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Tue Oct 4 09:36:40 2011 Subject: [Histonet] Von Kossa Stain for calcium Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A6B@PHSXMB30.partners.org> We are a research lab and don't do a big volume of special stains. We just received a project for 35 slides that need Von Kossa Stain. I acid-washed some small glass staining dishes-no rack required (in the past, we only had a few requests). Can I run slides in plastic slide holders (24-place) in an acid-washed beaker for the silver nitrate step? Any tips would be appreciated. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rgrow <@t> bmnet.com Tue Oct 4 09:48:25 2011 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Tue Oct 4 09:48:31 2011 Subject: [Histonet] Green counterstain problems Message-ID: Good Morning Histonetters! I've been having a counterstain problem with my GMS and PAS-F. Both stains work as they should until the green counterstain is applied. I cannot get my tissue (control or patient) to uptake the counterstain. I have varied time, temperature, even companies. I have good controls that have been in use for years without problems, until now. Only after an extended -10+ minutes,- can I get a blush of color in them. I've even called the slide company to ask if there has been a change to the charge coating I'm open to suggestions. Thanks! Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. From relia1 <@t> earthlink.net Tue Oct 4 09:51:50 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Oct 4 09:51:53 2011 Subject: [Histonet] RELIA Histology Careers Bulletin Special Edition for Managers and Supervisors  10-4-2011 Message-ID: Hi Histonetters!! What would make the perfect management position? Is it the location? Perhaps the type of lab? How about the size of the staff/lab? Maybe it?s the Hours? The Money and/or benefits? Could it be that a particular situation is the next logical step in your career? More than likely the answer is all of the above in different degrees. That is why I am contacting you with this special bulletin. I am starting to get a lot of management opportunities and I wanted to touch base and let you know. Your next opportunity might be just around the corner and I might have it for you. If you are looking for a position right now please contact me right away. We can talk about my current positions OR about a customized search on your behalf. If you aren?t looking right away but want to let me know what would make a perfect job for you so that I could keep an eye out, that would be great too. To do that just shoot me an email at relia1@earthlink.net or call me toll free at 866-607-3542. Here is a list of my current managerial opportunities: Pathology Lab Manager ? Modesto, CA Assistant Histology Supervisor ? Austin, TX Histology Supervisor ? Portland, OR Histology Manager ? Long Island, NY All of these clients offer autonomy in your position and histology staff eager to welcome their new manager. I really appreciate you taking time out of your busy day to read my e-mail Thanks Again! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From Lynne.Bell <@t> cvmc.org Tue Oct 4 09:56:43 2011 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Tue Oct 4 09:56:51 2011 Subject: [Histonet] Green counterstain problems In-Reply-To: References: Message-ID: We have had this problem before. Make sure that the glacial acetic acid has been added to the light green stock solution. Cured our problem!!! Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rgrow@bmnet.com Sent: Tuesday, October 04, 2011 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Green counterstain problems Good Morning Histonetters! I've been having a counterstain problem with my GMS and PAS-F. Both stains work as they should until the green counterstain is applied. I cannot get my tissue (control or patient) to uptake the counterstain. I have varied time, temperature, even companies. I have good controls that have been in use for years without problems, until now. Only after an extended -10+ minutes,- can I get a blush of color in them. I've even called the slide company to ask if there has been a change to the charge coating I'm open to suggestions. Thanks! Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Oct 4 10:02:33 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Oct 4 10:02:39 2011 Subject: [Histonet] RELIA Histology Careers Bulletin - Latest exciting opportunities with some of the best companies in the U.S. Message-ID: <58E718407D93481EA59083D739700A76@ownerf1abaad51> Hi Histonetters!! I hope everyone is having a great day. Here is a list of some of my most exciting opportunities for histology techs. My clients offer excellent pay, benefits and relocation assistance. These are full time permanent positions. Take a look and if you or someone you know might be interested in any of these positions or assistance with a search customized to an area not mentioned in this list let me know. I have been working exclusively in the permanent placement of histology professionals for almost 10 years and if there is a position in an area and I don't have it, I KNOW I can find it. Here are the position that I want to tell you about. *Histotechnologist/PA - Charlotte, NC - A RELIA Exclusive *Histology Tech - Portland OR - A RELIA Exclusive!! New GRADS Welcome to apply!!! *Histology Tech - Austin, TX **I also have positions in NY, CA, MA, MI and TN.** Take the opportunity yourself or help a friend and earn a 500 dollar referral fee. For more information please contact Pam Barker toll free at 866-607-3542 or via email at relia1@earthlink.net Enjoy Your Day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From MSHERWOOD <@t> PARTNERS.ORG Tue Oct 4 10:02:45 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Tue Oct 4 10:02:53 2011 Subject: [Histonet] Von Kossa stain kit Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A6C@PHSXMB30.partners.org> Thought of another question! What other vendors do people order special stain kits? We have been using Poly Scientific, which is very good, but costly. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From ratliffjack <@t> hotmail.com Tue Oct 4 10:19:33 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Oct 4 10:19:39 2011 Subject: [Histonet] Von Kossa stain kit In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A6C@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A6C@PHSXMB30.partners.org> Message-ID: It has been my experience that you do not need to acid wash the glassware for the silver nitrate step. The only critical parts are the time (5 minutes) in silver nitrate (protected from light), the 3 dH2O rinses (also protected from light), developing time (2 minutes) in sodium carbonate-formaldehyde solution (protected from light), 2 dH2O rinses (no need to protect from light), the 30 seconds in Farmer's Diminisher (sodium thiosulfate-potassium ferricyanide solution) and tap water rinse. The sodium-carbonate formaldehyde solution is use to chemically develop the silver attachment to any presence of mineralization. Most people use natural light, but the chemical step is more consistent in my opinion. Additionally, since the silver nitrate step is used to attach silver ions to the presence of minerals, the use of any metal is prohibited as it will also bind silver. However, if you use plastic, glass, or even stainless steel, no worries at all. As for a reliable kit, I use the kit from Dorn and Hart Microedge (www.dornandhart.com). You can also choose you preferred counterstain with this kit. I routinely work with mineralized bone and therefore use the Von Kossa - MacNeal's Tetrachrome stain kit. Hope this helps! Best, Jack > Date: Tue, 4 Oct 2011 11:02:45 -0400 > From: MSHERWOOD@PARTNERS.ORG > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Von Kossa stain kit > > Thought of another question! What other vendors do people order special stain > kits? We have been using Poly Scientific, which is very good, but costly. > > Thanks! > Peggy > > Peggy Sherwood > Lab Associate, Photopathology > Wellman Center for Photomedicine (EDR 214) > Massachusetts General Hospital > 50 Blossom Street > Boston, MA 02114-2696 > 617-724-4839 (voice mail) > 617-726-6983 (lab) > 617-726-1206 (fax) > msherwood@partners.org > > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From af46 <@t> buffalo.edu Tue Oct 4 10:36:54 2011 From: af46 <@t> buffalo.edu (Featherstone, Annette) Date: Tue Oct 4 10:36:58 2011 Subject: [Histonet] rabbit CD68 and Rabbit CD31 Message-ID: Is anyone using a rabbit CD68 or Rabbit CD31 on pig tissue and would you be willing to share your vendor and protocol? Annette Featherstone Supervisor SUNY @UB From rsrichmond <@t> gmail.com Tue Oct 4 10:38:41 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Oct 4 10:38:47 2011 Subject: [Histonet] Ann Preece (was "decal [sic] question") Message-ID: Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology Core Facility of the Robert. H. Lurie Cancer Center at Northwestern University in Chicago notes" >>Ann Preece states acid decal uses aqueous solutions of either formic, nitric, or trichloroacetic acid. Other methods mentioned are Ion-exchange resin, electrical ionization and chelation. The histo bible!<< You've got to be almost as geezer as me to remember when Ann Preece's "A Manual for Histologic Technicians" was the histo bible. I was fortunate to be able to purloin a pristine (no stain spills) copy of the third edition (1972) from the wreckage of an old histology lab about 20 years ago. Indeed, Patsy Ruegg! "Decal" is a trademark of the Decal Chemical Corporation and should not be used generically for decalcifying solutions. See decal-bone.com Bob Richmond Samurai Pathologist Knoxville TN From boneslides <@t> aol.com Tue Oct 4 10:38:48 2011 From: boneslides <@t> aol.com (boneslides@aol.com) Date: Tue Oct 4 10:39:15 2011 Subject: [Histonet] (no subject) Message-ID: <8CE50C2D7FE2A27-1704-49B8@Webmail-d124.sysops.aol.com> http://www.jewellerydropship.co.uk/mysite.php?html45 From Ronald.Houston <@t> nationwidechildrens.org Tue Oct 4 10:39:55 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Oct 4 10:40:06 2011 Subject: [Histonet] Von Kossa stain kit In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A6C@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A6C@PHSXMB30.partners.org> Message-ID: I've used Rowley Biochemical for years: http://rowleybio.com/ Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Tuesday, October 04, 2011 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Von Kossa stain kit Thought of another question! What other vendors do people order special stain kits? We have been using Poly Scientific, which is very good, but costly. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From mtighe <@t> trudeauinstitute.org Tue Oct 4 10:56:03 2011 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Tue Oct 4 10:54:40 2011 Subject: [Histonet] Tissue processor Message-ID: <4E8AF3E6.26E4.00EE.0@trudeauinstitute.org> I am looking to purchase a Processor and embedding center. I have received a quote for VIP6 and a TEC5 but they seem to be a bit out of our price range. Does anyone have another Processor/embedding center that they found to be reliable? Thanks Mike From rjbuesa <@t> yahoo.com Tue Oct 4 11:20:32 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 4 11:20:36 2011 Subject: [Histonet] Tissue processor In-Reply-To: <4E8AF3E6.26E4.00EE.0@trudeauinstitute.org> Message-ID: <1317745232.70494.YahooMailClassic@web65707.mail.ac4.yahoo.com> When you are buying VIP6 or TEC5 you are both buying pot notch technology + quality. For sure you will find others cheaper but of lesser quality. You should think of this as if buying a new car: Do you want to buy one cheaper but less reliable, or one that will give you years of reliable transportation? It is the same thing with histology instruments! Ren? J. --- On Tue, 10/4/11, Mike Tighe wrote: From: Mike Tighe Subject: [Histonet] Tissue processor To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 4, 2011, 11:56 AM I am looking to purchase a Processor and embedding center. I have received a quote for VIP6 and a TEC5 but they seem? to be a bit out of our price range. Does anyone have another Processor/embedding center that they found to be reliable? Thanks Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> cvmc.org Tue Oct 4 11:23:47 2011 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Tue Oct 4 11:23:51 2011 Subject: [Histonet] Ann Preece (was "decal [sic] question") In-Reply-To: References: Message-ID: I also have a copy of Preece's "bible" from 1972! A couple of pages are loose and the spine is a bit frayed. What a great book!! Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, October 04, 2011 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ann Preece (was "decal [sic] question") Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology Core Facility of the Robert. H. Lurie Cancer Center at Northwestern University in Chicago notes" >>Ann Preece states acid decal uses aqueous solutions of either formic, nitric, or trichloroacetic acid. Other methods mentioned are Ion-exchange resin, electrical ionization and chelation. The histo bible!<< You've got to be almost as geezer as me to remember when Ann Preece's "A Manual for Histologic Technicians" was the histo bible. I was fortunate to be able to purloin a pristine (no stain spills) copy of the third edition (1972) from the wreckage of an old histology lab about 20 years ago. Indeed, Patsy Ruegg! "Decal" is a trademark of the Decal Chemical Corporation and should not be used generically for decalcifying solutions. See decal-bone.com Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Tue Oct 4 11:28:48 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Oct 4 11:29:04 2011 Subject: [Histonet] Ann Preece (was "decal [sic] question") In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE238DEF82AAE@MERCERMAIL.MercerU.local> Oh I had a 1965 edition, but someone "borrowed" it and never brought it back. I have since acquired the 1972 edition in good shape, but there was a 1959 edition. That would be a good one to have in the library. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Tuesday, October 04, 2011 12:24 PM To: 'Bob Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ann Preece (was "decal [sic] question") I also have a copy of Preece's "bible" from 1972! A couple of pages are loose and the spine is a bit frayed. What a great book!! Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, October 04, 2011 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ann Preece (was "decal [sic] question") Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology Core Facility of the Robert. H. Lurie Cancer Center at Northwestern University in Chicago notes" >>Ann Preece states acid decal uses aqueous solutions of either formic, nitric, or trichloroacetic acid. Other methods mentioned are Ion-exchange resin, electrical ionization and chelation. The histo bible!<< You've got to be almost as geezer as me to remember when Ann Preece's "A Manual for Histologic Technicians" was the histo bible. I was fortunate to be able to purloin a pristine (no stain spills) copy of the third edition (1972) from the wreckage of an old histology lab about 20 years ago. Indeed, Patsy Ruegg! "Decal" is a trademark of the Decal Chemical Corporation and should not be used generically for decalcifying solutions. See decal-bone.com Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Tue Oct 4 11:34:32 2011 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue Oct 4 11:34:35 2011 Subject: [Histonet] RE: Von Kossa Stain for calcium In-Reply-To: <53d51152-8610-43f5-8349-9561a9c2dfaf@DCPWPRTR01.mdanderson.edu> References: <53d51152-8610-43f5-8349-9561a9c2dfaf@DCPWPRTR01.mdanderson.edu> Message-ID: When running more than 5-10 slides at a time in a silver stain, a precipitate will most likely form. It would be better to run them in small batches in coplin jars to give you the necessary control needed. Acid washing is fine, but just laboratory grade cleaner works as well. I usually place the slides in a coplin jar, under a lamp wrapped in foil, on a warm surface (hot plate, embedder, or even in a waterbath).For 35 slides I would stagger the start times to allow myself time to check them. The only extra step would be having multiple control slides. Running 24 slides at once can be daunting, so a few in multiple coplin jars would work better for me to handle. Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Message: 17 Date: Tue, 4 Oct 2011 10:36:30 -0400 From: "Sherwood, Margaret" Subject: Re: [Histonet] Von Kossa Stain for calcium To: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A6B@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" We are a research lab and don't do a big volume of special stains. We just received a project for 35 slides that need Von Kossa Stain. I acid-washed some small glass staining dishes-no rack required (in the past, we only had a few requests). Can I run slides in plastic slide holders (24-place) in an acid-washed beaker for the silver nitrate step? Any tips would be appreciated. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ From gilchrie <@t> DENTISTRY.UNC.EDU Tue Oct 4 11:05:10 2011 From: gilchrie <@t> DENTISTRY.UNC.EDU (Gilchrist, Eric) Date: Tue Oct 4 11:38:48 2011 Subject: [Histonet] RE: Slide/Block retention Message-ID: <6B0498A1D99925478E639D9FAD568C2B01861F8119@dentistrymail1.dentistry.unc.edu> CLIA-88 requirements can be found here: http://wwwn.cdc.gov/clia/regs/subpart_j.aspx. Local and state requirements may vary. Eric Gilchrist, Supervisor UNC Oral and Maxillofacial Pathology Laboratory -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, October 04, 2011 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 95, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Slide/Block Retention (Richard Cartun) 2. Cpt Charge (Anita Buchiane) 3. RE: Atlas of laboratory mouse histology (Hinsinger Julie) 4. RE: Cpt Charge (Weems, Joyce) 5. RE: decal question (Sarah Dysart) 6. RE: Cpt Charge (Setlak, Lisa) 7. RE: decal question (Bernice Frederick) 8. RE: decal question (Houston, Ronald) 9. RE: decal question (Patsy Ruegg) 10. Re: Slide/Block Retention (Bob Richmond) 11. RE: Atlas of laboratory mouse histology (Andrea Marion) 12. RE: Re: Slide/Block Retention (Blazek, Linda) 13. HP controls (Marcia Funk) 14. Atlas of laboratory mouse histology (Amos Brooks) 15. RE: decal question (Jennifer MacDonald) 16. RE: decal question (Edwards, Richard E.) 17. Re: Von Kossa Stain for calcium (Sherwood, Margaret) 18. Green counterstain problems (rgrow@bmnet.com) 19. RELIA Histology Careers Bulletin Special Edition for Managers and Supervisors  10-4-2011 (Pam Barker) 20. RE: Green counterstain problems (Bell, Lynne) ---------------------------------------------------------------------- Message: 1 Date: Mon, 03 Oct 2011 13:20:42 -0400 From: "Richard Cartun" Subject: Re: [Histonet] Slide/Block Retention To: , "Michael J Dessoye" Message-ID: <4E89B6AA.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII We currently keep slides and paraffin blocks (surgical pathology) for 17 years; I would keep more, but laboratory administration keeps tell me to discard because we don't have sufficient storage space. It's not unusual for me to receive a request for a block(s) from the 1990's for IHC, molecular, or genetic testing especially now that we have entered the arena for "Personalized Medicine". Also, I find myself re-testing specimens from the 1990's and 2000's with new generation antibodies, probes, and detection systems. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Dessoye, Michael J" 10/3/2011 9:50 AM >>> Hello Histonet, What policy is everyone following for slide and block retention? We are not CAP, however we currently keep slides and blocks for 10 years. My director wants to decrease that period to 2 years, which is the Joint Commission standard. I would like to keep 10 years because we frequently are asked to send slides for consult and review that are several years old. It is also helpful when looking for hard-to-find control tissue. However these reasons are apparently not sufficient. I'm leaning towards keeping the 10 year policy, but I need additional justification. Or does anyone think the 2 year period is sufficient? Thanks, Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 3 Oct 2011 17:24:16 +0000 From: Anita Buchiane Subject: [Histonet] Cpt Charge To: "histonet@lists.utsouthwestern.edu" Message-ID: <4034E71604330C4B8E10D1538DFB2455CD38@BMHEXCH02.bmhvt.org> Content-Type: text/plain; charset="us-ascii" Can we still only bill 88342 per antibody /per part or has that been changed? For instance: If we do a Calponin and an Actin on 3 blocks from one part do we charge 88342 x 2 or x 6? I thought I remember reading that it was changed to allow for charging per slide but I can not find the reference. Thanks _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ ------------------------------ Message: 3 Date: Mon, 3 Oct 2011 13:27:52 -0400 From: "Hinsinger Julie" Subject: [Histonet] RE: Atlas of laboratory mouse histology To: Message-ID: <070FB8DB1EFA214292C54DCBECEB27CF02566A8F@MAPIUDEM2.sim.umontreal.ca> Content-Type: text/plain; charset="iso-8859-1" Just to let you know about the first answers: - There is an online atlas that was brought to my attention : http://www.mouseatlas.org/data/mouse/libraries/SM202 - And most referred book was Atlas of Mouse Development by Matthew H. Kaufman with a revised edition, printed in 2002. (Good for mouse embryo stages) I might have another question: As for adult organs, is there a general histology atlas you refer to? In our lab, we use "Pathology of the Mouse" Robert R. Maronpot As we work on both human and mouse tissues, I have found a new book that might be interesting "Comparative Anatomy and Histology: A Mouse and Human Atlas" Have you heard about it? It will be released in December 2011. http://www.amazon.ca/Comparative-Anatomy-Histology-Expert-Consult/dp/0123813611/ref=sr_1_1?s=books&ie=UTF8&qid=1317661829&sr=1-1 It aims at the new mouse investigator as well as medical and veterinary pathologists who need to expand their knowledge base into comparative anatomy and histology. It guides the reader through normal mouse anatomy and histology using direct comparison to the human. The side by side comparison of mouse and human tissues highlight the unique biology of the mouse, which has great impact on the validation of mouse models of human disease. Seems great! I don't know... Julie De : Hinsinger Julie Envoy? : 3 octobre 2011 11:41 ? : 'histonet@lists.utsouthwestern.edu' Objet : Atlas of laboratory mouse histology Does anyone know of an atlas of mouse histology? Thanks for your comments Julie Hinsinger Histology Facility Manager IRIC - Pav. M. Coutu - Local 3440 2950, chemin polytechnique - Montr?al (Qc) H3T 1J4 ------------------------------ Message: 4 Date: Mon, 3 Oct 2011 13:54:23 -0400 From: "Weems, Joyce" Subject: [Histonet] RE: Cpt Charge To: Anita Buchiane , "histonet@lists.utsouthwestern.edu" Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640827B749CC@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" You can now charge per block. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anita Buchiane Sent: Monday, October 03, 2011 13:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cpt Charge Can we still only bill 88342 per antibody /per part or has that been changed? For instance: If we do a Calponin and an Actin on 3 blocks from one part do we charge 88342 x 2 or x 6? I thought I remember reading that it was changed to allow for charging per slide but I can not find the reference. Thanks _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 5 Date: Mon, 3 Oct 2011 17:55:04 +0000 From: Sarah Dysart Subject: RE: [Histonet] decal question To: Patsy Ruegg , "histonet@lists.utsouthwestern.edu" Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5079FA@SN2PRD0702MB110.namprd07.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Formalin? Isn't all decal acid decal? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 3 Oct 2011 13:01:04 -0500 From: "Setlak, Lisa" Subject: [Histonet] RE: Cpt Charge To: "'Weems, Joyce'" , Anita Buchiane , "histonet@lists.utsouthwestern.edu" Message-ID: <7111DB39D045004C9CF29E79C71B28BC1093D4D479@CMHEXCC01MBX.childrensmemorial.org> Content-Type: text/plain; charset="us-ascii" Where we can find a reference to this.... I know I'll get questions when I change it? Thanks, Lisa Children's Memorial Hospital Chicago, Il -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, October 03, 2011 12:54 PM To: Anita Buchiane; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cpt Charge You can now charge per block. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anita Buchiane Sent: Monday, October 03, 2011 13:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cpt Charge Can we still only bill 88342 per antibody /per part or has that been changed? For instance: If we do a Calponin and an Actin on 3 blocks from one part do we charge 88342 x 2 or x 6? I thought I remember reading that it was changed to allow for charging per slide but I can not find the reference. Thanks _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 3 Oct 2011 18:17:59 +0000 From: Bernice Frederick Subject: RE: [Histonet] decal question To: Patsy Ruegg , "histonet@lists.utsouthwestern.edu" Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E17A1B4@evcspmbx3.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" Ann Preece states acid decal uses aqueous solutions od either formic,nitric or trichloroacetic acid. Other methods mentioned are Ion-exchange resin,electrical ionization and chelation. The histo bible! Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 3 Oct 2011 18:25:32 +0000 From: "Houston, Ronald" Subject: RE: [Histonet] decal question To: 'Patsy Ruegg' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Alcoholic formalin or 70% ethanol as, quoting Gayle Callis, "alcohol can slow or prevent decalcification" Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 9 Date: Mon, 3 Oct 2011 12:25:52 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] decal question To: "'Sarah Dysart'" , Message-ID: <7104CA2AA7E64D859B3B1084AD323E5B@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I think I miss phrased this question, we thought they were asking what fixative should never be used if you are planning to do acid decal after fixation? We still think it must be osmium???? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Sarah Dysart [mailto:sdysart@mirnarx.com] Sent: Monday, October 03, 2011 11:55 AM To: Patsy Ruegg; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decal question Formalin? Isn't all decal acid decal? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 3 Oct 2011 14:30:26 -0400 From: Bob Richmond Subject: [Histonet] Re: Slide/Block Retention To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I don't care what the herrn inshpektors say, the fact is that patient care - with the rapid growth of genetic and other molecular techniques - I think 10 years is the minimum for retaining paraffin blocks, and I'd be a lot more comfortable with 20. In the future I expect we'll be retrieving blocks to do studies on members of patients' families. And if your kid flunks out of college, I reckon you can sue the OB that delivered him - so that's >20 years for placentas. An issue rarely mentioned: when a tissue lab closes, everything is often discarded. We need requirements for tissue retention when labs close. I have no idea how this could be done. But as regulations increase and our economy declines, there'll be a lot of tissue labs closing in the next decade. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 11 Date: Mon, 3 Oct 2011 14:34:07 -0500 From: "Andrea Marion" Subject: [Histonet] RE: Atlas of laboratory mouse histology To: histonet@lists.utsouthwestern.edu Cc: julie.hinsinger@umontreal.ca Message-ID: <0c00e9909cf1a85757b0f687b639347f.squirrel@webmail.uic.edu> Content-Type: text/plain;charset=iso-8859-1 Hi Julie - Other than the ones you already found, I have two (okay 3) more recommendations: 1- Theiler's classic The House Mouse: Atlas of Embryonic Development has a great histology of mouse embryos. I use it side-by-side with the Kaufmann text for embryo histology. It's freely available for download, which is very handy: http://www.emouseatlas.org/Atlas/Theiler_book_download.html 2- There is a quick online guide for adult mouse histology at http://ctrgenpath.net/static/atlas/mousehistology/Windows/introduction.html It's a nice page for quickly checking something, but there is not a lot of in depth coverage. 3 - I also suggest searching on the mouse research listserv, I've seen it discussed there before and a quick search turned up a number of recommendations: http://www.informatics.jax.org/lyris-cgi/lyris.pl?visit=mgi-list Good luck, Andrea Andrea Marion Graduate Student University of Illinois at Chicago [Histonet] RE: Atlas of laboratory mouse histology Hinsinger Julie julie.hinsinger <@t> umontreal.ca Mon Oct 3 12:27:52 CDT 2011 Previous message: [Histonet] RE: Cpt Charge Next message: [Histonet] decal question Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] Just to let you know about the first answers: - There is an online atlas that was brought to my attention : http://www.mouseatlas.org/data/mouse/libraries/SM202 - And most referred book was Atlas of Mouse Development by Matthew H. Kaufman with a revised edition, printed in 2002. (Good for mouse embryo stages) I might have another question: As for adult organs, is there a general histology atlas you refer to? In our lab, we use "Pathology of the Mouse" Robert R. Maronpot As we work on both human and mouse tissues, I have found a new book that might be interesting "Comparative Anatomy and Histology: A Mouse and Human Atlas" Have you heard about it? It will be released in December 2011. http://www.amazon.ca/Comparative-Anatomy-Histology-Expert-Consult/dp/0123813611/ref=sr_1_1?s=books&ie=UTF8&qid=1317661829&sr=1-1 It aims at the new mouse investigator as well as medical and veterinary pathologists who need to expand their knowledge base into comparative anatomy and histology. It guides the reader through normal mouse anatomy and histology using direct comparison to the human. The side by side comparison of mouse and human tissues highlight the unique biology of the mouse, which has great impact on the validation of mouse models of human disease. Seems great! I don't know... Julie De : Hinsinger Julie Envoy? : 3 octobre 2011 11:41 ? : 'histonet <@t> lists.utsouthwestern.edu' Objet : Atlas of laboratory mouse histology Does anyone know of an atlas of mouse histology? Thanks for your comments Julie Hinsinger Histology Facility Manager IRIC - Pav. M. Coutu - Local 3440 2950, chemin polytechnique - Montr?al (Qc) H3T 1J4 ------------------------------ Message: 12 Date: Mon, 3 Oct 2011 15:50:15 -0400 From: "Blazek, Linda" Subject: RE: [Histonet] Re: Slide/Block Retention To: 'Bob Richmond' , "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E39127401B36E@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" There is an inspection question that addresses what happens to material if a lab closes. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Monday, October 03, 2011 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Slide/Block Retention I don't care what the herrn inshpektors say, the fact is that patient care - with the rapid growth of genetic and other molecular techniques - I think 10 years is the minimum for retaining paraffin blocks, and I'd be a lot more comfortable with 20. In the future I expect we'll be retrieving blocks to do studies on members of patients' families. And if your kid flunks out of college, I reckon you can sue the OB that delivered him - so that's >20 years for placentas. An issue rarely mentioned: when a tissue lab closes, everything is often discarded. We need requirements for tissue retention when labs close. I have no idea how this could be done. But as regulations increase and our economy declines, there'll be a lot of tissue labs closing in the next decade. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 03 Oct 2011 16:20:34 -0400 From: "Marcia Funk" Subject: [Histonet] HP controls To: Message-ID: <4E89D2C2.E948.00AC.1@mercyhealth.com> Content-Type: text/plain; charset=US-ASCII We are having difficult with the HP control for IHC staining - If you are purchasing controls or if you have controls that you might share I would really appreciate your help. Thanks so much -Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 ------------------------------ Message: 14 Date: Mon, 3 Oct 2011 17:27:28 -0400 From: Amos Brooks Subject: [Histonet] Atlas of laboratory mouse histology To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, I would be interested in this reference as well. Please post to the group if you have any suggestions. Amos On Mon, Oct 3, 2011 at 1:00 PM, wrote: > Message: 10 > Date: Mon, 3 Oct 2011 11:40:55 -0400 > From: "Hinsinger Julie" > Subject: [Histonet] Atlas of laboratory mouse histology > To: > Message-ID: > < > 070FB8DB1EFA214292C54DCBECEB27CF02566A13@MAPIUDEM2.sim.umontreal.ca> > Content-Type: text/plain; charset="iso-8859-1" > > Does anyone know of an atlas of mouse histology? > > Thanks for your comments > > > > Julie Hinsinger > > Histology Facility Manager > ------------------------------ Message: 15 Date: Tue, 4 Oct 2011 04:46:50 -0700 From: Jennifer MacDonald Subject: RE: [Histonet] decal question To: "Patsy Ruegg" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Was there more to the question? You would not want to use any fixatives containing metals, such as mercury, if you will be doing end-point decalcification determination using x-rays. "Patsy Ruegg" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/03/2011 11:27 AM To "'Sarah Dysart'" , cc Subject RE: [Histonet] decal question I think I miss phrased this question, we thought they were asking what fixative should never be used if you are planning to do acid decal after fixation? We still think it must be osmium???? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Sarah Dysart [mailto:sdysart@mirnarx.com] Sent: Monday, October 03, 2011 11:55 AM To: Patsy Ruegg; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decal question Formalin? Isn't all decal acid decal? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 4 Oct 2011 13:17:15 +0100 From: "Edwards, Richard E." Subject: RE: [Histonet] decal question To: 'Jennifer MacDonald' , Patsy Ruegg Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4EDF88502@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Was it decalcifying a tissue fixed in a formalin based fixative using a hydrochloric acid containing decalcifying fluid? as even storage of HCl and formalin in the same area is a bad plan. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: 04 October 2011 12:47 To: Patsy Ruegg Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] decal question Was there more to the question? You would not want to use any fixatives containing metals, such as mercury, if you will be doing end-point decalcification determination using x-rays. "Patsy Ruegg" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/03/2011 11:27 AM To "'Sarah Dysart'" , cc Subject RE: [Histonet] decal question I think I miss phrased this question, we thought they were asking what fixative should never be used if you are planning to do acid decal after fixation? We still think it must be osmium???? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Sarah Dysart [mailto:sdysart@mirnarx.com] Sent: Monday, October 03, 2011 11:55 AM To: Patsy Ruegg; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decal question Formalin? Isn't all decal acid decal? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Tue, 4 Oct 2011 10:36:30 -0400 From: "Sherwood, Margaret" Subject: Re: [Histonet] Von Kossa Stain for calcium To: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A6B@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" We are a research lab and don't do a big volume of special stains. We just received a project for 35 slides that need Von Kossa Stain. I acid-washed some small glass staining dishes-no rack required (in the past, we only had a few requests). Can I run slides in plastic slide holders (24-place) in an acid-washed beaker for the silver nitrate step? Any tips would be appreciated. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 18 Date: Tue, 4 Oct 2011 10:48:25 -0400 From: rgrow@bmnet.com Subject: [Histonet] Green counterstain problems To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Good Morning Histonetters! I've been having a counterstain problem with my GMS and PAS-F. Both stains work as they should until the green counterstain is applied. I cannot get my tissue (control or patient) to uptake the counterstain. I have varied time, temperature, even companies. I have good controls that have been in use for years without problems, until now. Only after an extended -10+ minutes,- can I get a blush of color in them. I've even called the slide company to ask if there has been a change to the charge coating I'm open to suggestions. Thanks! Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. ------------------------------ Message: 19 Date: Tue, 4 Oct 2011 10:51:50 -0400 From: "Pam Barker" Subject: [Histonet] RELIA Histology Careers Bulletin Special Edition for Managers and Supervisors  10-4-2011 To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters!! What would make the perfect management position? Is it the location? Perhaps the type of lab? How about the size of the staff/lab? Maybe it?s the Hours? The Money and/or benefits? Could it be that a particular situation is the next logical step in your career? More than likely the answer is all of the above in different degrees. That is why I am contacting you with this special bulletin. I am starting to get a lot of management opportunities and I wanted to touch base and let you know. Your next opportunity might be just around the corner and I might have it for you. If you are looking for a position right now please contact me right away. We can talk about my current positions OR about a customized search on your behalf. If you aren?t looking right away but want to let me know what would make a perfect job for you so that I could keep an eye out, that would be great too. To do that just shoot me an email at relia1@earthlink.net or call me toll free at 866-607-3542. Here is a list of my current managerial opportunities: Pathology Lab Manager ? Modesto, CA Assistant Histology Supervisor ? Austin, TX Histology Supervisor ? Portland, OR Histology Manager ? Long Island, NY All of these clients offer autonomy in your position and histology staff eager to welcome their new manager. I really appreciate you taking time out of your busy day to read my e-mail Thanks Again! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 20 Date: Tue, 4 Oct 2011 10:56:43 -0400 From: "Bell, Lynne" Subject: RE: [Histonet] Green counterstain problems To: "'rgrow@bmnet.com'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We have had this problem before. Make sure that the glacial acetic acid has been added to the light green stock solution. Cured our problem!!! Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rgrow@bmnet.com Sent: Tuesday, October 04, 2011 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Green counterstain problems Good Morning Histonetters! I've been having a counterstain problem with my GMS and PAS-F. Both stains work as they should until the green counterstain is applied. I cannot get my tissue (control or patient) to uptake the counterstain. I have varied time, temperature, even companies. I have good controls that have been in use for years without problems, until now. Only after an extended -10+ minutes,- can I get a blush of color in them. I've even called the slide company to ask if there has been a change to the charge coating I'm open to suggestions. Thanks! Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 95, Issue 4 *************************************** From sbreeden <@t> nmda.nmsu.edu Tue Oct 4 11:40:51 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Oct 4 11:40:57 2011 Subject: [Histonet] Tissue processor In-Reply-To: <1317745232.70494.YahooMailClassic@web65707.mail.ac4.yahoo.com> References: <4E8AF3E6.26E4.00EE.0@trudeauinstitute.org> <1317745232.70494.YahooMailClassic@web65707.mail.ac4.yahoo.com> Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFB0E@nmdamailsvr.nmda.ad.nmsu.edu> Although I appreciate individual opinions, do not overlook the Leica line. Us "old timers" may recognize the names "Sakura" or "Thermo" (in its many permutations) more readily, I'd put my money on Leica every time (and I have - with processor, embedding center, microtome and autostainer). They do not let me down. I have no issues with either other vendor, but I placed my trust in German engineering. I'm just sayin'... From thomas.crowell <@t> novartis.com Tue Oct 4 11:44:17 2011 From: thomas.crowell <@t> novartis.com (Crowell, Thomas) Date: Tue Oct 4 11:44:25 2011 Subject: [Histonet] Paraffin sections for molecular assays Message-ID: Dear Histonetters, Can you tell me what procedures you use to prevent DNA/RNA contamination of tissue sections when handling multiple samples. Do you just wipe down blade holder and blade (or use a new blade) between samples, or do you use more stringent cleaning? Thomas Crowell Diagnostic Development Novartis Molecular Diagnostics 45 Sidney Street Cambridge, MA 02139 USA Phone +1 617 8717460 Fax +1 N.A. thomas.crowell@novartis.com www.novartis.com From JWeems <@t> sjha.org Tue Oct 4 11:58:29 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Oct 4 11:58:34 2011 Subject: [Histonet] Ann Preece (was "decal [sic] question") In-Reply-To: <9BF995BC0E47744E9673A41486E24EE238DEF82AAE@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE238DEF82AAE@MERCERMAIL.MercerU.local> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640827BDDC7C@CHEXCMS10.one.ads.che.org> I still have my 1965 version.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, October 04, 2011 12:29 To: Bell, Lynne; 'Bob Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ann Preece (was "decal [sic] question") Oh I had a 1965 edition, but someone "borrowed" it and never brought it back. I have since acquired the 1972 edition in good shape, but there was a 1959 edition. That would be a good one to have in the library. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Tuesday, October 04, 2011 12:24 PM To: 'Bob Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ann Preece (was "decal [sic] question") I also have a copy of Preece's "bible" from 1972! A couple of pages are loose and the spine is a bit frayed. What a great book!! Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, October 04, 2011 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ann Preece (was "decal [sic] question") Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology Core Facility of the Robert. H. Lurie Cancer Center at Northwestern University in Chicago notes" >>Ann Preece states acid decal uses aqueous solutions of either formic, >>nitric, or trichloroacetic acid. Other methods mentioned are >>Ion-exchange resin, electrical ionization and chelation. The histo >>bible!<< You've got to be almost as geezer as me to remember when Ann Preece's "A Manual for Histologic Technicians" was the histo bible. I was fortunate to be able to purloin a pristine (no stain spills) copy of the third edition (1972) from the wreckage of an old histology lab about 20 years ago. Indeed, Patsy Ruegg! "Decal" is a trademark of the Decal Chemical Corporation and should not be used generically for decalcifying solutions. See decal-bone.com Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From micropathlabs <@t> yahoo.com Tue Oct 4 12:07:42 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Tue Oct 4 12:07:46 2011 Subject: [Histonet] Tissue processor In-Reply-To: <4E8AF3E6.26E4.00EE.0@trudeauinstitute.org> References: <4E8AF3E6.26E4.00EE.0@trudeauinstitute.org> Message-ID: <1317748062.24151.YahooMailNeo@web161714.mail.bf1.yahoo.com> We purchased a processor this past year and went with the VIP6. We demo'd several but went with the VIP because of its reliability and quality. We own two older models, the oldest over 20 years old,?that still run well. We also liked the Leica model that was slightly less, but I was able to convince administration the VIP was worth the difference. ? Hope this helps. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ? From: Mike Tighe To: histonet@lists.utsouthwestern.edu Sent: Tuesday, October 4, 2011 11:56 AM Subject: [Histonet] Tissue processor I am looking to purchase a Processor and embedding center. I have received a quote for VIP6 and a TEC5 but they seem? to be a bit out of our price range. Does anyone have another Processor/embedding center that they found to be reliable? Thanks Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Tue Oct 4 12:22:59 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Oct 4 12:23:16 2011 Subject: [Histonet] Ann Preece (was "decal [sic] question") In-Reply-To: References: Message-ID: <4940DF6D1C5FDF48931B6966AAEF939524A7CC@chimsx08.CHI.catholichealth.net> Ah, yes. That edition featured "105 illustrations, including 2 four-color plates" (back in the day when that kind of stuff was worth mentioning on the title page) Have a great day - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, October 04, 2011 10:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ann Preece (was "decal [sic] question") Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology Core Facility of the Robert. H. Lurie Cancer Center at Northwestern University in Chicago notes" >>Ann Preece states acid decal uses aqueous solutions of either formic, >>nitric, or trichloroacetic acid. Other methods mentioned are >>Ion-exchange resin, electrical ionization and chelation. The histo >>bible!<< You've got to be almost as geezer as me to remember when Ann Preece's "A Manual for Histologic Technicians" was the histo bible. I was fortunate to be able to purloin a pristine (no stain spills) copy of the third edition (1972) from the wreckage of an old histology lab about 20 years ago. Indeed, Patsy Ruegg! "Decal" is a trademark of the Decal Chemical Corporation and should not be used generically for decalcifying solutions. See decal-bone.com Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Tue Oct 4 12:31:56 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Oct 4 12:32:00 2011 Subject: [Histonet] =?iso-8859-1?q?Re=3A_von_K=F3ssa_method_for_calcium?= Message-ID: Why is the von K?ssa method for calcified tissue still in use? It's not very specific and requires handling silver. Why not alizarin red, a simple dye that is more specific. I've seen alizarin red S (C.I. 58005) used to demonstrate microcalcifications in breast biopsy specimens. Obviously one can see the more common calcium phosphate calcifications in the H & E, but the less common calcium oxalate deposits (which are also visible to the radiologist) are nearly invisible. I actually prefer polarization to see calcium oxalate, but many pathologists don't have access to polarizers. Bob Richmond Samurai Pathologist Knoxville TN From hfedor <@t> jhmi.edu Tue Oct 4 12:36:24 2011 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Tue Oct 4 12:36:29 2011 Subject: [Histonet] RE: Paraffin sections for molecular assays In-Reply-To: References: Message-ID: Hello, For DNA, we use a new blande and we wipe down microtome, handles, front of blade holder with 95% ethanol on a gauze sponge. For RNA, use a new blade and wipe down all with RNAzap including the water bath and rinse with DEPC water. Use clean water in bath, distilled will work or DEPC water. Depending on the stringency of the assay. You can sometimes get away with a little less. But some of the assays that are being run can cost up to $1,000.00. So the time spent cleaning up is justified. Make sure you are wearing gloves for both RNA and DNA, during the whole process, even when handling the slides .(including the process of labeling slides). I usually lay the slides out on pape rtowels on the lab bench while labeling them. Helen Fedor Johns Hopkins University TMA and Prostate Spore Lab Manager JHU SOM Uro/Pathology 600 N. Wolfe Street, Marburg Room 406 Baltimore MD, 21287-7065 410-614-1660 / Fax 410-614-3695 Pager 410-283-3419 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Crowell, Thomas [thomas.crowell@novartis.com] Sent: Tuesday, October 04, 2011 12:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin sections for molecular assays Dear Histonetters, Can you tell me what procedures you use to prevent DNA/RNA contamination of tissue sections when handling multiple samples. Do you just wipe down blade holder and blade (or use a new blade) between samples, or do you use more stringent cleaning? Thomas Crowell Diagnostic Development Novartis Molecular Diagnostics 45 Sidney Street Cambridge, MA 02139 USA Phone +1 617 8717460 Fax +1 N.A. thomas.crowell@novartis.com www.novartis.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Tue Oct 4 12:52:42 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Oct 4 12:52:45 2011 Subject: [Histonet] Tissue processor In-Reply-To: <1317748062.24151.YahooMailNeo@web161714.mail.bf1.yahoo.com> References: <4E8AF3E6.26E4.00EE.0@trudeauinstitute.org> <1317748062.24151.YahooMailNeo@web161714.mail.bf1.yahoo.com> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32096B49B7@Mail2Node2.ad.uams.edu> We are just getting our second Excelsior and love them. It cuts tech time changing bottles and paraffin as well as keeping us on track daily for changes and rotations. We have to VIP, that are very old and one just died. That is the one we are replacing with Thermo. The VIPs are great units just could not go the price and reagent exposure with them. We also have Leica ASP300 love but skipped it for the same reasons. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Tuesday, October 04, 2011 12:08 PM To: Mike Tighe; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue processor We purchased a processor this past year and went with the VIP6. We demo'd several but went with the VIP because of its reliability and quality. We own two older models, the oldest over 20 years old,?that still run well. We also liked the Leica model that was slightly less, but I was able to convince administration the VIP was worth the difference. ? Hope this helps. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ? From: Mike Tighe To: histonet@lists.utsouthwestern.edu Sent: Tuesday, October 4, 2011 11:56 AM Subject: [Histonet] Tissue processor I am looking to purchase a Processor and embedding center. I have received a quote for VIP6 and a TEC5 but they seem? to be a bit out of our price range. Does anyone have another Processor/embedding center that they found to be reliable? Thanks Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From Wanda.Smith <@t> HCAhealthcare.com Tue Oct 4 12:56:26 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Tue Oct 4 12:56:28 2011 Subject: [Histonet] SCSHT Fall Meeting at Marina Inn at Grande Dunes Reservations Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA272FF78B2F@NADCWPMSGCMS03.hca.corpad.net> Anyone planning to attend the Fall Meeting of the South Carolina Society of Histotechnology at Marina Inn in Myrtle Beach, SC November 4-5, 2011 NEEDS TO MAKE YOUR RESERVATIONS!!! Number to call is 877-403-7676 and mention SCSHT for special rate. SC Histotechs and other members of SCSHT, this is an election year, so we need as many of you as possible to turn out and GET INVOLVED!!! Anyone needing a copy of the program, let me know and I can email you a copy. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From tpodawiltz <@t> lrgh.org Tue Oct 4 13:27:23 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Oct 4 13:27:41 2011 Subject: [Histonet] Ann Preece (was "decal [sic] question") In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF939524A7CC@chimsx08.CHI.catholichealth.net> References: <4940DF6D1C5FDF48931B6966AAEF939524A7CC@chimsx08.CHI.catholichealth.net> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DF2EA93E@LRGHEXVS1.practice.lrgh.org> I had a dachshund named Willey, he ate my copy, guess he like the pictures. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, October 04, 2011 1:23 PM To: Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ann Preece (was "decal [sic] question") Ah, yes. That edition featured "105 illustrations, including 2 four-color plates" (back in the day when that kind of stuff was worth mentioning on the title page) Have a great day - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, October 04, 2011 10:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ann Preece (was "decal [sic] question") Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology Core Facility of the Robert. H. Lurie Cancer Center at Northwestern University in Chicago notes" >>Ann Preece states acid decal uses aqueous solutions of either formic, >>nitric, or trichloroacetic acid. Other methods mentioned are >>Ion-exchange resin, electrical ionization and chelation. The histo >>bible!<< You've got to be almost as geezer as me to remember when Ann Preece's "A Manual for Histologic Technicians" was the histo bible. I was fortunate to be able to purloin a pristine (no stain spills) copy of the third edition (1972) from the wreckage of an old histology lab about 20 years ago. Indeed, Patsy Ruegg! "Decal" is a trademark of the Decal Chemical Corporation and should not be used generically for decalcifying solutions. See decal-bone.com Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From billodonnell <@t> catholichealth.net Tue Oct 4 13:29:06 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Oct 4 13:29:23 2011 Subject: [Histonet] Ann Preece (was "decal [sic] question") In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DF2EA93E@LRGHEXVS1.practice.lrgh.org> References: <4940DF6D1C5FDF48931B6966AAEF939524A7CC@chimsx08.CHI.catholichealth.net> <38667E7FB77ECD4E91BFAEB8D986386323DF2EA93E@LRGHEXVS1.practice.lrgh.org> Message-ID: <4940DF6D1C5FDF48931B6966AAEF939524A7E1@chimsx08.CHI.catholichealth.net> Was Willey the dog you and sue had in VA? Seems he ate everything - including an electrical cord - -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Tuesday, October 04, 2011 1:27 PM To: O'Donnell, Bill; Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ann Preece (was "decal [sic] question") I had a dachshund named Willey, he ate my copy, guess he like the pictures. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, October 04, 2011 1:23 PM To: Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ann Preece (was "decal [sic] question") Ah, yes. That edition featured "105 illustrations, including 2 four-color plates" (back in the day when that kind of stuff was worth mentioning on the title page) Have a great day - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, October 04, 2011 10:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ann Preece (was "decal [sic] question") Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology Core Facility of the Robert. H. Lurie Cancer Center at Northwestern University in Chicago notes" >>Ann Preece states acid decal uses aqueous solutions of either formic, >>nitric, or trichloroacetic acid. Other methods mentioned are >>Ion-exchange resin, electrical ionization and chelation. The histo >>bible!<< You've got to be almost as geezer as me to remember when Ann Preece's "A Manual for Histologic Technicians" was the histo bible. I was fortunate to be able to purloin a pristine (no stain spills) copy of the third edition (1972) from the wreckage of an old histology lab about 20 years ago. Indeed, Patsy Ruegg! "Decal" is a trademark of the Decal Chemical Corporation and should not be used generically for decalcifying solutions. See decal-bone.com Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From mbplab <@t> yahoo.com Tue Oct 4 13:55:13 2011 From: mbplab <@t> yahoo.com (Mary Benoit) Date: Tue Oct 4 13:56:17 2011 Subject: [Histonet] Congo Red for Amyloid Message-ID: <1317754513.40283.YahooMailNeo@web43137.mail.sp1.yahoo.com> Need help with Congo Red for Amyloid problem.? We have been using automated staining with Dako's Artisan and recently tried manual procedure with fresh reagents from Polyscientific kit with little to no staining on two commercially prepared controls and two different patients who have been sent for consult and returned with a positive amyloidosis diagnosis( as predicted by my pathologist).? I followed procedure furnished by Polyscientific to the "T", cut slides at 8 microns, used clean fresh reagents and glassware.? I did notice that Polyscientific's order of reagent application was different from old AFIP manual of staining instructions and the time in Congo Red a little shorter( 20 minutes as opposed to 1 hour).? Any suggestions?? Mary F Benoit MT(ASCP) The Pathology Laboratory Lake Charles, Louisiana mbplab@yahoo.com From tpodawiltz <@t> lrgh.org Tue Oct 4 15:57:29 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Oct 4 15:58:04 2011 Subject: [Histonet] Ann Preece (was "decal [sic] question") In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF939524A7E1@chimsx08.CHI.catholichealth.net> References: <4940DF6D1C5FDF48931B6966AAEF939524A7CC@chimsx08.CHI.catholichealth.net> <38667E7FB77ECD4E91BFAEB8D986386323DF2EA93E@LRGHEXVS1.practice.lrgh.org>, <4940DF6D1C5FDF48931B6966AAEF939524A7E1@chimsx08.CHI.catholichealth.net> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DEA0CE6E@LRGHEXVS1.practice.lrgh.org> Yes he was, and yes he did Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: O'Donnell, Bill [billodonnell@catholichealth.net] Sent: Tuesday, October 04, 2011 2:29 PM To: Podawiltz, Thomas; Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ann Preece (was "decal [sic] question") Was Willey the dog you and sue had in VA? Seems he ate everything - including an electrical cord - -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Tuesday, October 04, 2011 1:27 PM To: O'Donnell, Bill; Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ann Preece (was "decal [sic] question") I had a dachshund named Willey, he ate my copy, guess he like the pictures. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, October 04, 2011 1:23 PM To: Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ann Preece (was "decal [sic] question") Ah, yes. That edition featured "105 illustrations, including 2 four-color plates" (back in the day when that kind of stuff was worth mentioning on the title page) Have a great day - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, October 04, 2011 10:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ann Preece (was "decal [sic] question") Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology Core Facility of the Robert. H. Lurie Cancer Center at Northwestern University in Chicago notes" >>Ann Preece states acid decal uses aqueous solutions of either formic, >>nitric, or trichloroacetic acid. Other methods mentioned are >>Ion-exchange resin, electrical ionization and chelation. The histo >>bible!<< You've got to be almost as geezer as me to remember when Ann Preece's "A Manual for Histologic Technicians" was the histo bible. I was fortunate to be able to purloin a pristine (no stain spills) copy of the third edition (1972) from the wreckage of an old histology lab about 20 years ago. Indeed, Patsy Ruegg! "Decal" is a trademark of the Decal Chemical Corporation and should not be used generically for decalcifying solutions. See decal-bone.com Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From amosbrooks <@t> gmail.com Tue Oct 4 18:25:28 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Oct 4 18:25:32 2011 Subject: [Histonet] Von Kossa stain kit Message-ID: Kits ... Bah! Just make it up. Save yourself a fortune and actually know what's in the solutions. All the classical texts should have the procedures as well as various websites like stainsfile.org & IHCworld.com Otherwise just ask here and I'm sure someone will have the information you need (with much debate as is the nature of the histonet). Kits! (rolling eyes & grinning) Amos On Tue, Oct 4, 2011 at 12:39 PM, wrote: > Message: 2 > Date: Tue, 4 Oct 2011 11:02:45 -0400 > From: "Sherwood, Margaret" > Subject: Re: [Histonet] Von Kossa stain kit > To: > Message-ID: > <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A6C@PHSXMB30.partners.org> > Content-Type: text/plain; charset="us-ascii" > > Thought of another question! What other vendors do people order special > stain > kits? We have been using Poly Scientific, which is very good, but costly. > > Thanks! > Peggy > From amosbrooks <@t> gmail.com Tue Oct 4 18:38:33 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Oct 4 18:38:38 2011 Subject: [Histonet] Paraffin sections for molecular assays Message-ID: Hi, You will want to do more than just wiping it down. You should use something specifically designed to clean such contamination like RNAase away (Invitrogen if memory serves). You'll want to wipe down any surfaces that come in contact with the block, sections or your hands when sectioning. Don't forget your forceps & probe or whatever you use and wear gloves. Just because I'm obsessive compulsive, I dip the block it's self into the stuff. It is best to float the sections on molecular biology grade water or de-ionized water that has been treated with DEPC. Molecular water is cheaper and don't stink like DEPC! Good luck, Amos On Tue, Oct 4, 2011 at 1:00 PM, wrote: > Message: 2 > Date: Tue, 4 Oct 2011 16:44:17 +0000 > From: "Crowell, Thomas" > Subject: [Histonet] Paraffin sections for molecular assays > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > BC3645844188C945830E826F2FF0977D014B59FA@023-CH1MPN1-022.023d.mgd.msft.net > > > > Content-Type: text/plain; charset="us-ascii" > > Dear Histonetters, > > Can you tell me what procedures you use to prevent DNA/RNA contamination of > tissue sections when handling multiple samples. Do you just wipe down blade > holder and blade (or use a new blade) between samples, or do you use more > stringent cleaning? > > > Thomas Crowell > From amitapandey <@t> torrentpharma.com Tue Oct 4 23:11:14 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Tue Oct 4 23:13:49 2011 Subject: [Histonet] rabbit CD68 and Rabbit CD31 In-Reply-To: References: Message-ID: I am using rabbit CD31 (sc1506-R) from santacruz on rat tissue and it is working fine. Dr. Amita Dubey PCSED,TRC From: "Featherstone, Annette" To: "'histonet@lists.utsouthwestern.edu'" Date: 04/10/11 09:06 PM Subject: [Histonet] rabbit CD68 and Rabbit CD31 Sent by: histonet-bounces@lists.utsouthwestern.edu Is anyone using a rabbit CD68 or Rabbit CD31 on pig tissue and would you be willing to share your vendor and protocol? Annette Featherstone Supervisor SUNY @UB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From H.J.G.vandeKant <@t> uu.nl Wed Oct 5 01:10:33 2011 From: H.J.G.vandeKant <@t> uu.nl (Kant, H.J.G. van de (Henk)) Date: Wed Oct 5 01:10:45 2011 Subject: [Histonet] Tlr 9 immuno Message-ID: <8A014D954ADDDD44AC39749D8EB677C9A98C7D@ICTSC-W-S205.soliscom.uu.nl> Hi All, Can somebody help me with ordering a TLR 9 antibody, which is working on mouse tissue, formalin fixed and paraffin embedded. Including the procedure and the name of the company. Greetings, Henk van de Kant Utrecht Institute of Pharmaceutical Sciences Faculty of Sciences, Utrecht University visiting address: Universiteitsweg 99, room 2.213584CG Utrecht, the Netherlands mailing address: PO Box 80.195, 3508TB Utrecht The Netherlands From louise.renton <@t> gmail.com Wed Oct 5 02:42:26 2011 From: louise.renton <@t> gmail.com (Louise Renton) Date: Wed Oct 5 02:42:38 2011 Subject: [Histonet] "decal [sic] question" Message-ID: funny, I thought a decal was a sort of transfer for decorating things with - like guitars and hot rods - what others call "stickers" hard to trademark a word like that! On Tue, Oct 4, 2011 at 5:38 PM, Bob Richmond wrote: > Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology > Core Facility of the Robert. H. Lurie Cancer Center at Northwestern > University in Chicago notes" > > >>Ann Preece states acid decal uses aqueous solutions of either formic, > nitric, or trichloroacetic acid. Other methods mentioned are Ion-exchange > resin, electrical ionization and chelation. The histo bible!<< > > You've got to be almost as geezer as me to remember when Ann Preece's > "A Manual for Histologic Technicians" was the histo bible. I was > fortunate to be able to purloin a pristine (no stain spills) copy of > the third edition (1972) from the wreckage of an old histology lab > about 20 years ago. > > Indeed, Patsy Ruegg! "Decal" is a trademark of the Decal Chemical > Corporation and should not be used generically for decalcifying > solutions. See decal-bone.com > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From DKBoyd <@t> chs.net Wed Oct 5 07:57:38 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Oct 5 07:57:47 2011 Subject: [Histonet] Ann Preece (was "decal [sic] question") In-Reply-To: Message-ID: I have both a 1959 and 1972 copy of Preece's book! Both were left by the previous Histology Manager. What a treasurer for me. I also used Preece when studying for the registry. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Bob Richmond Sent by: histonet-bounces@lists.utsouthwestern.edu 10/04/2011 11:39 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Ann Preece (was "decal [sic] question") Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology Core Facility of the Robert. H. Lurie Cancer Center at Northwestern University in Chicago notes" >>Ann Preece states acid decal uses aqueous solutions of either formic, nitric, or trichloroacetic acid. Other methods mentioned are Ion-exchange resin, electrical ionization and chelation. The histo bible!<< You've got to be almost as geezer as me to remember when Ann Preece's "A Manual for Histologic Technicians" was the histo bible. I was fortunate to be able to purloin a pristine (no stain spills) copy of the third edition (1972) from the wreckage of an old histology lab about 20 years ago. Indeed, Patsy Ruegg! "Decal" is a trademark of the Decal Chemical Corporation and should not be used generically for decalcifying solutions. See decal-bone.com Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From gu.lang <@t> gmx.at Wed Oct 5 10:05:16 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Oct 5 10:05:24 2011 Subject: AW: [Histonet] Paraffin sections for molecular assays In-Reply-To: References: Message-ID: Hi! For our KRAS-testing we clean the microtome with LTK008, especially surfaces that come in contact with the sections. We use one-way-toothpicks, single packed, to pick the slide from the blade and give it directly into a sterile collection tube. 1-10 sections are collected depending on the dimensions of the tumorarea. In this way we don't have to deal with to-clean or not-to-clean water in the waterbath, brushes, forceps etc. Gudrun From anna_hughes <@t> merck.com Wed Oct 5 14:26:18 2011 From: anna_hughes <@t> merck.com (Hughes, Anna) Date: Wed Oct 5 14:26:23 2011 Subject: [Histonet] Skin punch biopsy Message-ID: <0B49B3779F179945B48D29E7793B2A037642E41381@USCTMXP51014.merck.com> Hi! I have 2mm skin punch biopsies that I need to process, but it has been a very long time since I have done this and don't really remember the times/station. These biopsies have been cut in half so they are extremely small - In other words, I am only planning on processing 1/2 of the biopsy punch. What do you guys recommend for a processing schedule? Thanks! Anna Hughes Anna C. Hughes Merck & Co., Inc. anna_hughes@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From lpjones <@t> srhs-pa.org Wed Oct 5 14:43:40 2011 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Wed Oct 5 14:43:43 2011 Subject: [Histonet] Labvision Autostainer Message-ID: <4AE8039AEA096143B965CBC6D092166802351B3961@EXCH2007.srhs-pa.org> Good afternoon fellow Histotechs! We have been using our Labvision Autostainer for 5 or so years now, and love the open system. Recently, we had a rep from Fisher come and do a PM on it, because we noticed the amount of reagent in some bottles was not changing at all, while others were running out very quickly. Today I took off a run of slides, and not one drop of DAB had been used. The stainer does not alarm, but its obviously not drawing up solution in some cases. Mr. Fisher rep said it tested fine. Has anyone else experienced this? We would greatly appreciate any insight or advice. Thanks so much! Laura Jones & The Histochicks @ Sharon Regional Health System ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From kmilne <@t> bccancer.bc.ca Wed Oct 5 15:19:57 2011 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Wed Oct 5 15:20:02 2011 Subject: [Histonet] Isotype specific polymers In-Reply-To: References: Message-ID: <3FEFF18FF4E1914A9AB7D8498591BE8610F149E424@VEXCCR02.phsabc.ehcnet.ca> Hi everyone, I'm curious if there is such a thing as isotype specific polymers (ie will react with mouse IgG2a but not IgG1). Does anyone make such a thing? Thanks, Katy From histotech411 <@t> gmail.com Wed Oct 5 18:03:30 2011 From: histotech411 <@t> gmail.com (Jenny Vega) Date: Wed Oct 5 18:03:33 2011 Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% Message-ID: Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to putrefied if they are put in that solution. Am I wrong or is she wrong? thanks From chapcl <@t> yahoo.com Wed Oct 5 18:05:31 2011 From: chapcl <@t> yahoo.com (William) Date: Wed Oct 5 18:05:46 2011 Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% In-Reply-To: References: Message-ID: Oh dear. Yes they are the same. Sent from my iPhone On Oct 5, 2011, at 4:03 PM, Jenny Vega wrote: > Ok I want to know the diferrence between Neutral Buffered Formalin 10% and > NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same > thing and in the book from Frieda Carson it says that but my supervisor > swears they are different chemicals. In the laboratory she has used 10% > neutral buffered formalin all the time, but when we ran out of it we were > sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB > formaldehyde 3.7 to 4% once again. She says that 10% NBF is more > concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are > going to putrefied if they are put in that solution. > > Am I wrong or is she wrong? thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosie_scrimizzi <@t> hotmail.com Wed Oct 5 18:51:08 2011 From: rosie_scrimizzi <@t> hotmail.com (Rosie Scrimizzi) Date: Wed Oct 5 18:51:12 2011 Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% In-Reply-To: References: , Message-ID: concentrated formaldehyde is 37-40% w/v so 10% formalin is 3.7-4% w/v.........same thing...... yes......oh dear!! > From: chapcl@yahoo.com > Date: Wed, 5 Oct 2011 16:05:31 -0700 > To: histotech411@gmail.com > Subject: Re: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% > CC: histonet@lists.utsouthwestern.edu > > Oh dear. Yes they are the same. > > Sent from my iPhone > > On Oct 5, 2011, at 4:03 PM, Jenny Vega wrote: > > > Ok I want to know the diferrence between Neutral Buffered Formalin 10% and > > NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same > > thing and in the book from Frieda Carson it says that but my supervisor > > swears they are different chemicals. In the laboratory she has used 10% > > neutral buffered formalin all the time, but when we ran out of it we were > > sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB > > formaldehyde 3.7 to 4% once again. She says that 10% NBF is more > > concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are > > going to putrefied if they are put in that solution. > > > > Am I wrong or is she wrong? thanks > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapcl <@t> yahoo.com Wed Oct 5 18:59:25 2011 From: chapcl <@t> yahoo.com (William) Date: Wed Oct 5 18:59:35 2011 Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% In-Reply-To: References: Message-ID: <440E87D9-FE94-46E9-B469-821A5057D674@yahoo.com> Jenni, we do not "oh dear" you, in fact we congratulate you for coming here to get the answers you need. Many of us lament the state of affairs in histology today. Formaldehyde can not exist as a liquid. 100% Formaldehyde is a gas. To use it in histology we purchase it as formalin which has a concentration of 37% to a max of 40% formaldehyde. We then make 10% formalin which is 3.7% to 4% formaldehyde. Incidentally I have worked with a pathologist who used nothing but 4% zinc formalin very successfully (actually better penetration than 10%NBF) -- invalidating the initial premise of your supervisor. Will Sent from my iPhone On Oct 5, 2011, at 4:51 PM, Rosie Scrimizzi wrote: > concentrated formaldehyde is 37-40% w/v so 10% formalin is 3.7-4% w/v.........same thing...... > > yes......oh dear!! > > > From: chapcl@yahoo.com > > Date: Wed, 5 Oct 2011 16:05:31 -0700 > > To: histotech411@gmail.com > > Subject: Re: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% > > CC: histonet@lists.utsouthwestern.edu > > > > Oh dear. Yes they are the same. > > > > Sent from my iPhone > > > > On Oct 5, 2011, at 4:03 PM, Jenny Vega wrote: > > > > > Ok I want to know the diferrence between Neutral Buffered Formalin 10% and > > > NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same > > > thing and in the book from Frieda Carson it says that but my supervisor > > > swears they are different chemicals. In the laboratory she has used 10% > > > neutral buffered formalin all the time, but when we ran out of it we were > > > sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB > > > formaldehyde 3.7 to 4% once again. She says that 10% NBF is more > > > concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are > > > going to putrefied if they are put in that solution. > > > > > > Am I wrong or is she wrong? thanks > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mamawooo <@t> hotmail.com Wed Oct 5 19:11:25 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Wed Oct 5 19:11:29 2011 Subject: [Histonet] (no subject) Message-ID: Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets. There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue. The answers were surprisingly pretty much the same. It is not an issue! Now I understand how one company can make this claim as their stainer uses fresh stain on each slide. The explanations from the other companies were insulting and just plain did not make sense to me. I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath." Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places. I have seen them from the doctor's office or procedure room to the stainer and every step in between. Sometimes if the "floater" is in the block it is very difficult to determine where it originated. We can however eliminate the water bath and stainer as the origin in these cases. One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle. I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes. So, no, not all floaters float. I would love to hear feedback from others on this. I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it. I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha From joelleweaver <@t> hotmail.com Wed Oct 5 21:38:23 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Wed Oct 5 21:38:27 2011 Subject: [Histonet] Difference between Neutral Buffered Formalin 10% andNB formaldehyde 3.7 to 4% Message-ID: I considered mentioning the gas state of formaldehyde, and its saturation potential in aqueous solution, so I am glad to see this was posted.. However I was hesitant to reply right away b/c I too was sad that you were given this misinformation, especially since it is so easy to look up and verify. Good for you in not accepting an answer that did not seem correct and seeking more info! Sent from my Verizon Wireless BlackBerry -----Original Message----- From: William Date: Wed, 5 Oct 2011 23:59:25 To: Cc: ; ; Subject: Re: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% Jenni, we do not "oh dear" you, in fact we congratulate you for coming here to get the answers you need.? Many of us lament the state of affairs in histology today. Formaldehyde can not exist as a liquid.?? 100% Formaldehyde is a gas.? To use it in histology we purchase it as formalin which has a concentration of 37% to a max of 40% formaldehyde. We then make 10% formalin which is 3.7% to 4% formaldehyde. Incidentally I have worked with a pathologist who used nothing but 4% zinc formalin very successfully (actually better penetration than 10%NBF) -- invalidating the initial premise of your supervisor. Will Sent from my iPhone On Oct 5, 2011, at 4:51 PM, Rosie Scrimizzi wrote: > concentrated formaldehyde is 37-40% w/v so 10% formalin is 3.7-4% w/v.........same thing...... > > yes......oh dear!! > > > From: chapcl@yahoo.com > > Date: Wed, 5 Oct 2011 16:05:31 -0700 > > To: histotech411@gmail.com > > Subject: Re: [Histonet] Difference between Neutral Buffered Formalin 10% and??????? NB formaldehyde 3.7 to 4% > > CC: histonet@lists.utsouthwestern.edu > > > > Oh dear. Yes they are the same. > > > > Sent from my iPhone > > > > On Oct 5, 2011, at 4:03 PM, Jenny Vega wrote: > > > > > Ok I want to know the diferrence between Neutral Buffered Formalin 10% and > > > NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same > > > thing and in the book from Frieda Carson it says that but my supervisor > > > swears they are different chemicals. In the laboratory she has used 10% > > > neutral buffered formalin all the time, but when we ran out of it we were > > > sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB > > > formaldehyde 3.7 to 4% once again. She says that 10% NBF is more > > > concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are > > > going to putrefied if they are put in that solution. > > > > > > Am I wrong or is she wrong? thanks > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Thu Oct 6 03:00:57 2011 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Thu Oct 6 03:01:10 2011 Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% In-Reply-To: References: Message-ID: <7722595275A4DD4FA225B92CDBF174A101A4EDF88526@EXC-MBX3.cfs.le.ac.uk> The answer, get another supervisor, she is obviously not up to it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: 06 October 2011 00:04 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to putrefied if they are put in that solution. Am I wrong or is she wrong? thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Carmen.M.Garcia <@t> uv.es Thu Oct 6 03:17:49 2011 From: Carmen.M.Garcia <@t> uv.es (Carmen Maria Garcia Pascual) Date: Thu Oct 6 03:18:01 2011 Subject: [Histonet] DAPI question Message-ID: <9186875781carmaga6@uv.es> Good morning: I am Carmen Garcia from the Faculty of Medicine of the University of Valencia, Spain. I usually use your vectashield mounting medium for fluorescence with DAPI (H-1200) and I never have any problem. But now I am working with 50um frozen sections and the problem that I have is that the DAPI staining does not arrive to the bottom of the section. Do you have any sugesstion of what can I do? (I already have the sections permeabilized with triton x-100) I have thougth to incubate with the mounting medium some hours at 4?C or put some mounting medium in the antibody diluent and incubate... any suggestion would be really welcome. Thank you so much, Kind regards, Carmen From idimitro <@t> mun.ca Thu Oct 6 06:58:53 2011 From: idimitro <@t> mun.ca (idimitro@mun.ca) Date: Thu Oct 6 07:38:04 2011 Subject: [Histonet] Cutting frozen sections from visceral fat Message-ID: <14C3108E8B98EF43B3EDAE5833670034050FD5@exchange.med.mun.ca> Hi everyone, I am having trouble cutting frozen sections from visceral fat. I have never cut only fat before. I am also cutting liver sections and they are perfect, but when I try to cut the fat it is not working. The temperature of the cryostat is ( -20) degree Celsius. I wonder if anyone has ever done this. I was thinking that if I lower the temperature or fix the fat with formol-calcium I may have success. Iliana Dimitrova, MSc, RT Histology Supervisor Room 2808 Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University St. John's, NL This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php From christiegowan <@t> msn.com Thu Oct 6 07:44:35 2011 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Thu Oct 6 07:44:44 2011 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: I agree with you Jan. In my 30 + years as a histotech (yes, I am old too) I have seen floaters come from a variety of places but I am hard pressed to remember any floater coming from the water bath. Today we are blessed with DNA fingerprinting to determine if the floater is or is not from the patient but that still does not address the real issue of where did it come from and how do we stop it. The vendors stating that it is not an issue have never been re-biopsied because of a floater in with their tissue. Good discussion and long overdue. I look forward to the day when it is no longer an automatic response from all involved that it is "Histology's fault". Hope you are enjoying your new adventures in retirement. See you next year in Canada! > From: mamawooo@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 5 Oct 2011 14:11:25 -1000 > Subject: [Histonet] (no subject) > > > Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets. There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue. The answers were surprisingly pretty much the same. It is not an issue! Now I understand how one company can make this claim as their stainer uses fresh stain on each slide. The explanations from the other companies were insulting and just plain did not make sense to me. I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath." Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places. I have seen them from the doctor's office or procedure room to the stainer and every step in between. Sometimes if the "floater" is in the block it is very difficult to determine where it originated. We can however eliminate the water bath and stainer as the origin in these cases. One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle. I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes. So, no, not all floaters float. I would love to hear feedback from others on this. I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it. I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From idimitro <@t> mun.ca Thu Oct 6 07:54:26 2011 From: idimitro <@t> mun.ca (idimitro@mun.ca) Date: Thu Oct 6 07:54:38 2011 Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% In-Reply-To: <440E87D9-FE94-46E9-B469-821A5057D674@yahoo.com> References: <440E87D9-FE94-46E9-B469-821A5057D674@yahoo.com> Message-ID: <14C3108E8B98EF43B3EDAE583367003405101C@exchange.med.mun.ca> Jenni, I am sending this link that should clarify it to your supervisor, although you are in a difficult position. Yes they are the same thing. Citation:" A fixative labeled as 10% buffered formalin is actually only a 4% solution of formaldehyde. This is because 10% buffered formalin is an example of old-time histologist's jargon describing a 10% solution made from a stock bottle of 37-40% formaldehyde (or more precisely: a 3.7-4% solution of formaldehyde).End of citation. http://swehsc.pharmacy.arizona.edu/exppath/resources/formaldehyde.php iliana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William Sent: October 5, 2011 9:29 PM To: Rosie Scrimizzi Cc: ; Subject: Re: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% Jenni, we do not "oh dear" you, in fact we congratulate you for coming here to get the answers you need. Many of us lament the state of affairs in histology today. Formaldehyde can not exist as a liquid. 100% Formaldehyde is a gas. To use it in histology we purchase it as formalin which has a concentration of 37% to a max of 40% formaldehyde. We then make 10% formalin which is 3.7% to 4% formaldehyde. Incidentally I have worked with a pathologist who used nothing but 4% zinc formalin very successfully (actually better penetration than 10%NBF) -- invalidating the initial premise of your supervisor. Will Sent from my iPhone On Oct 5, 2011, at 4:51 PM, Rosie Scrimizzi wrote: > concentrated formaldehyde is 37-40% w/v so 10% formalin is 3.7-4% w/v.........same thing...... > > yes......oh dear!! > > > From: chapcl@yahoo.com > > Date: Wed, 5 Oct 2011 16:05:31 -0700 > > To: histotech411@gmail.com > > Subject: Re: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% > > CC: histonet@lists.utsouthwestern.edu > > > > Oh dear. Yes they are the same. > > > > Sent from my iPhone > > > > On Oct 5, 2011, at 4:03 PM, Jenny Vega wrote: > > > > > Ok I want to know the diferrence between Neutral Buffered Formalin 10% and > > > NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same > > > thing and in the book from Frieda Carson it says that but my supervisor > > > swears they are different chemicals. In the laboratory she has used 10% > > > neutral buffered formalin all the time, but when we ran out of it we were > > > sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB > > > formaldehyde 3.7 to 4% once again. She says that 10% NBF is more > > > concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are > > > going to putrefied if they are put in that solution. > > > > > > Am I wrong or is she wrong? thanks > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php From rjbuesa <@t> yahoo.com Thu Oct 6 08:12:55 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 6 08:13:02 2011 Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% In-Reply-To: Message-ID: <1317906775.25229.YahooMailClassic@web65703.mail.ac4.yahoo.com> Jenny: Formaldehyde is?the GAS methanal?that dissolves in water at a maximum concentration between 37-40% vol./vol. To prevent its polymerization, small amounts of mathanol (an alcohol)are added. The 10% solution formaldehyde contains between 3.7 and 4.0% of formaldehyde and is called "formalin" in many countries and "formol" in Europe. So formalin is a 3.7 to 4.0% solution of formaldehyde. So a 10% formalin = 3.7-4.0% formaldehyde. NBF is prepared by added phosphate salts to prevent the formalin to acidify and is used to fix tissues. It is a pitty that your supervisor has so little knowledge about the area she is supposed to supervise, but I am not do not know why, but I am not.?surprised. To your question: SHE is wrong, really wrong! Ren? J. ? --- On Wed, 10/5/11, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 5, 2011, 7:03 PM Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it? we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to? putrefied if they are put in that solution. Am I wrong or is she wrong? thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abright <@t> brightinstruments.com Thu Oct 6 08:24:21 2011 From: abright <@t> brightinstruments.com (abright@brightinstruments.com) Date: Thu Oct 6 08:24:30 2011 Subject: [Histonet] Cutting frozen sections from visceral fat In-Reply-To: <14C3108E8B98EF43B3EDAE5833670034050FD5@exchange.med.mun.ca> References: <14C3108E8B98EF43B3EDAE5833670034050FD5@exchange.med.mun.ca> Message-ID: <1111148683-1317907463-cardhu_decombobulator_blackberry.rim.net-1484218883-@b27.c2.bise7.blackberry> You need to be at or below -28 degs C, also make sure that your knife and anti-roll plate are at or below this temperature too. Best regards Alan Bright Bright Instrument Co. Ltd. St Margarets Way Huntingdon Cambridgeshire PE29 6EU www.brightinsruments.com Sent from my BlackBerry? wireless device -----Original Message----- From: Sender: histonet-bounces@lists.utsouthwestern.edu Date: Thu, 6 Oct 2011 11:58:53 To: Subject: [Histonet] Cutting frozen sections from visceral fat Hi everyone, I am having trouble cutting frozen sections from visceral fat. I have never cut only fat before. I am also cutting liver sections and they are perfect, but when I try to cut the fat it is not working. The temperature of the cryostat is ( -20) degree Celsius. I wonder if anyone has ever done this. I was thinking that if I lower the temperature or fix the fat with formol-calcium I may have success. Iliana Dimitrova, MSc, RT Histology Supervisor Room 2808 Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University St. John's, NL This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING-LINKS ------------------------------------------------------ Teach SpamSniper if this mail (ID 01FFoF7eL) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01FFoF7eL&m=bc910a912ee7&t=20111006&c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01FFoF7eL&m=bc910a912ee7&t=20111006&c=n Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01FFoF7eL&m=bc910a912ee7&t=20111006&c=f ------------------------------------------------------ END-ANTISPAM-VOTING-LINKS From robinsoc <@t> mercyhealth.com Thu Oct 6 08:26:18 2011 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Thu Oct 6 08:26:28 2011 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <4E8D6628.D67D.00AF.1@mercyhealth.com> It is a good thing this vendor does not work in a histo lab because with that comment/attitude he/she would not last long. I have heard the water bath theory but upon investigation this has never been the source because every histotech I work with cleans it each and every time. We have traced floaters back to grossing stations, processors...especially placenta getting 'snagged' on a piece of bone...embedding forceps (we now use ones with no grooves), forcep warmers, stains and coverslippers. We have significantly reduced the number we see since we make the effort to track each one found as an ongoing QA project, but we still see the occasional floater. We did find that we have to put tissue types which fragment easily into mesh cassettes or bags but this can cause issues with fluid exchange and carryover during processing. It is a balancing act. I have always wondering about the newer processors with orientation cassettes which are embed and then cut without opening. Do they see less floaters with this type of 'closed' system? Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com >>> CHRISTIE GOWAN 10/6/2011 7:44 AM >>> I agree with you Jan. In my 30 + years as a histotech (yes, I am old too) I have seen floaters come from a variety of places but I am hard pressed to remember any floater coming from the water bath. Today we are blessed with DNA fingerprinting to determine if the floater is or is not from the patient but that still does not address the real issue of where did it come from and how do we stop it. The vendors stating that it is not an issue have never been re-biopsied because of a floater in with their tissue. Good discussion and long overdue. I look forward to the day when it is no longer an automatic response from all involved that it is "Histology's fault". Hope you are enjoying your new adventures in retirement. See you next year in Canada! > From: mamawooo@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 5 Oct 2011 14:11:25 -1000 > Subject: [Histonet] (no subject) > > > Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets. There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue. The answers were surprisingly pretty much the same. It is not an issue! Now I understand how one company can make this claim as their stainer uses fresh stain on each slide. The explanations from the other companies were insulting and just plain did not make sense to me. I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath." Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places. I have seen them from the doctor's office or procedure room to the stainer and every step in between. Sometimes if the "floater" is in the block it is very difficult to determine where it originated. We can however eliminate the water bath and stainer as the origin in these cases. One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle. I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes. So, no, not all floaters float. I would love to hear feedback from others on this. I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it. I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Oct 6 08:33:44 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 6 08:33:52 2011 Subject: [Histonet] Floaters In-Reply-To: Message-ID: <1317908024.35201.YahooMailClassic@web65703.mail.ac4.yahoo.com> This is a very interesting subject indeed. To start I always take "with a grain of salt" whatever a vendor tells me because their opinions may (or may not, but just in case) biased to the products they sell. The same thing goes for any automobiles salesperson of any automobile make claiming the "wonders" of the make they sell compared with other makes. Now, to the floater. I have also seen pieces of sections on the bottom of staining dishes. They are there because they fell-off the stained section. My question is: what are the possibilities of that piece of lost stained section that fell from its original slide, "re-float" and magically adhere to another slide? I really think that the possibilities range from almost none, to none. Going to the water bath: yes, this is a major source of floaters because a piece of a section left in the water bath can be picked up in another slide along with a section from another case. That is why it is mandatory to swipe the surface of the water bath with? a tissue after each case is cut. The source can be defined if we keep a record of the sequence in which the blocks are cut (as was standard procedure at my lab). Whenever we ended with a floater in a section we went immediately to the block to determine if the floater came from the block or the water bath. The other sources, either during description, cassetting or embedding are going to end in the block and it is very difficult to determine the origin. The only thing to do is to make sure that embedding wells where the forceps rest and heat, are thoroughly cleaned when the embedding ends. But I think that the main thrust of the question deals with floaters coming from the staining dishes: as I wrote earlier I give to this source?a very slim and almost impossible chance. Ren? J. ? ? ? --- On Wed, 10/5/11, Janice Mahoney wrote: From: Janice Mahoney Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 5, 2011, 8:11 PM Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets.? There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue.? The answers were surprisingly pretty much the same.???It is not an issue!? Now I understand how one company can make this claim as their stainer uses fresh stain on each slide.? The explanations from the other companies were insulting and just plain did not make sense to me.? I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath."? Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places.? I have seen them from the doctor's office or procedure room to the stainer and every step in between.? Sometimes if the "floater" is in the block it is very difficult to determine where it originated.? We can however eliminate the water bath and stainer as the origin in these cases.? One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle.? I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes.? So, no, not all floaters float.? I would love to hear feedback from others on this.? I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it.? I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MHillmer <@t> dermwisconsin.com Thu Oct 6 08:38:46 2011 From: MHillmer <@t> dermwisconsin.com (Michael Hillmer) Date: Thu Oct 6 08:38:50 2011 Subject: [Histonet] FW: Job Opening In-Reply-To: References: Message-ID: We are looking for a certified HT or HTL, to join our pathology lab in Manitowoc, WI. We currently have two (2) fantastic 1st shift positions open. We are seeking an experienced lab manager for a Monday-Friday position. This would be a salaried position and pay would be commensurate with experience. We are also seeking a 1st shift Ht or Htl (or someone who is eligilble to sit for the exam) to work 4 days a week, with the shift starting at 6am. We have excellent benefits, state-of-the-are lab (less than a year old) and a great team to work with. We will assist with relocation. Please contact me if you have any questions. Michael Hillmer PHR HR Coordinator Dermatology Associates of Wisconsin Phone: (920)683-5278 Fax: (920)663-9004 Cell: (920)860-6360 mhillmer@dermwisconsin.com . ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. From rcharles <@t> pa.gov Thu Oct 6 09:05:14 2011 From: rcharles <@t> pa.gov (Charles, Roger) Date: Thu Oct 6 09:05:20 2011 Subject: [Histonet] Tissue left in processor Message-ID: <3809C163DC1DA54AA534B3C7794D07B68EC20DC73B@ENHBGMBX01.PA.LCL> Hi All, Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes? And if so what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing was completed? Thanks so much. Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us From strombley <@t> THOCC.ORG Thu Oct 6 09:08:21 2011 From: strombley <@t> THOCC.ORG (Trombley, Sean) Date: Thu Oct 6 09:08:33 2011 Subject: [Histonet] JOB OPENING Message-ID: <01BF042B33B713429E44B7B92CA28F384C6D121D@CCHA-00SV0H0E02.nbgh.org> Hi All, We are currently searching for a part time person. The Hospital of Central Connecticut at New Britain General has an excellent opportunity for an experienced Histology Technologist to practice in our full service laboratory which consists of hematology, chemistry, microbiology, molecular diagnostics, histology, anatomic pathology and transfusion service. Our newly renovated state-of-the-art histology laboratory performs approximately 13,000 surgical cases each year and includes fully automated microtomes and an Immunohistochemistry stainer. Because our technologists are not responsible for grossing or frozen sectioning, they can fully focus on Histology. Qualifications: We are currently seeking flexible service technologists who will be available for flexible scheduling or 20hours/week Monday - Friday between 5:00 a.m. and 5:00 p.m., with some weekend availability. Requirements include current certification as a Histology Technician (HT) or Histotechnologist (HTL) through the American Society of Clinical Pathology (ASCP). One year of experience is preferred. For more information and to apply online, we invite you to visit our website at www.thocc.org. We are an Affirmative Action/Equal Opportunity Employer Thanks Sean Trombley (BS HTL QIHC ASCP) This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From rjbuesa <@t> yahoo.com Thu Oct 6 09:13:54 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 6 09:14:02 2011 Subject: [Histonet] Tissue left in processor In-Reply-To: <3809C163DC1DA54AA534B3C7794D07B68EC20DC73B@ENHBGMBX01.PA.LCL> Message-ID: <1317910434.70026.YahooMailClassic@web65710.mail.ac4.yahoo.com> I do not think that a well fixed, well processed tissue left in molten paraffin for 2 hours after the processor finished will have any adverse outcome. Ren? J. --- On Thu, 10/6/11, Charles, Roger wrote: From: Charles, Roger Subject: [Histonet] Tissue left in processor To: "Histonet (histonet@lists.utsouthwestern.edu)" Date: Thursday, October 6, 2011, 10:05 AM Hi All, Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes?? And if so? what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing was completed? Thanks so much. Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Thu Oct 6 09:17:43 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Thu Oct 6 09:17:48 2011 Subject: [Histonet] Tissue left in processor In-Reply-To: <3809C163DC1DA54AA534B3C7794D07B68EC20DC73B@ENHBGMBX01.PA.LCL> References: <3809C163DC1DA54AA534B3C7794D07B68EC20DC73B@ENHBGMBX01.PA.LCL> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A8C@PHSXMB30.partners.org> I don't think you want to leave it in the processor for 2 hours. Even at the embedding center, if someone can't come to help orient the tissue, we then take the cassettes out of the paraffin station and leave on a paper towel. Have done that for sometimes 2-3 days. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Thursday, October 06, 2011 10:05 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Tissue left in processor Hi All, Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes? And if so what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing was completed? Thanks so much. Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Gudrun.Erikstad <@t> stolav.no Thu Oct 6 09:22:55 2011 From: Gudrun.Erikstad <@t> stolav.no (Erikstad, Gudrun Hovstein) Date: Thu Oct 6 09:23:02 2011 Subject: SV: [Histonet] Difference between Neutral Buffered Formalin 10% and NBformaldehyde 3.7 to 4% In-Reply-To: <1317906775.25229.YahooMailClassic@web65703.mail.ac4.yahoo.com> Message-ID: I suppose Ren? meant to say: Formalin is a 37-40% solution of formaldehyde. 10% formalin is a 3,7-4,0% solution of formaldehyde. I've never heard the use of "Formol", but then again, I'm scandinavian, not som much european... ;-) Gudrun, Norway -----Opprinnelig melding----- Fra: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] P? vegne av Rene J Buesa Sendt: 6. oktober 2011 15:13 Til: histonet@lists.utsouthwestern.edu; Jenny Vega Emne: [Histonet] Difference between Neutral Buffered Formalin 10% and NBformaldehyde 3.7 to 4% Jenny: Formaldehyde is?the GAS methanal?that dissolves in water at a maximum concentration between 37-40% vol./vol. To prevent its polymerization, small amounts of mathanol (an alcohol)are added. The 10% solution formaldehyde contains between 3.7 and 4.0% of formaldehyde and is called "formalin" in many countries and "formol" in Europe. So formalin is a 3.7 to 4.0% solution of formaldehyde. So a 10% formalin = 3.7-4.0% formaldehyde. NBF is prepared by added phosphate salts to prevent the formalin to acidify and is used to fix tissues. It is a pitty that your supervisor has so little knowledge about the area she is supposed to supervise, but I am not do not know why, but I am not.?surprised. To your question: SHE is wrong, really wrong! Ren? J. ? --- On Wed, 10/5/11, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 5, 2011, 7:03 PM Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it? we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to? putrefied if they are put in that solution. Am I wrong or is she wrong? thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu Oct 6 09:31:53 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Oct 6 09:32:04 2011 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <4E8D8398.2B7F.00C9.1@geisinger.edu> I too spoke to one vendor who claimed that the floaters are not an issue and that their bottles drew below the level of the "floating" floaters. I didn't feel insulted, but did feel that this salesperson obviously had no actual lab experience or they never would have made such a ridiculous claim. I always put the opinions and experiences of other Histotechs far ahead of anything I hear from sales reps. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 >>> Janice Mahoney 10/5/2011 8:11 PM >>> Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets. There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue. The answers were surprisingly pretty much the same. It is not an issue! Now I understand how one company can make this claim as their stainer uses fresh stain on each slide. The explanations from the other companies were insulting and just plain did not make sense to me. I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath." Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places. I have seen them from the doctor's office or procedure room to the stainer and every step in between. Sometimes if the "floater" is in the block it is very difficult to determine where it originated. We can however eliminate the water bath and stainer as the origin in these cases. One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle. I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes. So, no, not all floaters float. I would love to hear feedback from others on this. I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it. I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From sbreeden <@t> nmda.nmsu.edu Thu Oct 6 09:56:55 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Oct 6 09:57:11 2011 Subject: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% In-Reply-To: References: <1317906775.25229.YahooMailClassic@web65703.mail.ac4.yahoo.com> Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFB21@nmdamailsvr.nmda.ad.nmsu.edu> Now, wait just a minute! Don't tell me that after 40+ years of being a histotech that I've been WRONG all this time??? FORMALDEHYDE is FORMALDEHYDE is FORMALDEHYDE. FORMALIN is the diluted form of FORMALDEHYDE, buffered and used at (usually) 10%. If I'm wrong, this will be my last day at work... :) From Ken_Marissael <@t> vwr.com Thu Oct 6 10:01:12 2011 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Thu Oct 6 10:01:26 2011 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 10/06/2011 and will not return until 10/12/2011. I will be away on 10/06 and return 10/12. I will have limited e-mail and cell phone access, but will try to get back to you as quickly as possible. While I am away, please contact VWR Healthcare Customer Service at 877-881-1192. If you require immediate attenion, please contact Jackie Zerillo at 609-410-6152. She is the NYC rep. From Timothy.Morken <@t> ucsfmedctr.org Thu Oct 6 10:19:25 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Oct 6 10:19:45 2011 Subject: [Histonet] Floaters In-Reply-To: <1317908024.35201.YahooMailClassic@web65703.mail.ac4.yahoo.com> References: <1317908024.35201.YahooMailClassic@web65703.mail.ac4.yahoo.com> Message-ID: <8D7C2D242DBD45498006B21122072BF89448A27E@MCINFRWEM003.ucsfmedicalcenter.org> The recent paper by Platt et all (Tissue Floaters and Contaminants in the Histology Laboratory, Arch Pathol Lab Med, Vol 133, June 2009) details floaters from waterbaths and staining process pretty well. Waterbaths do not appear to be a contributor. They literally found only ONE tissue fragment in waterbath water saved from an entire day of sectioning from 13 waterbaths. And it was in the water, not on a slide. They apparently did not identify ANY waterbath-derived floaters on any slide. They attribute this finding to the fact the tissue is still in paraffin so the tissue was either on the slide or stuck to the side of the waterbath, not floating in the water. To determine the extent of staining-derived floaters they measured the amount of tissue fragments found in each staining station. The vast majority were in the first xylene and alcohol baths. After the 95% alcohol there were only very rare tissue fragments in the rest of the baths. They found the staining process does contribute floaters (besides those that are clearly in the block), but apparently the do not derive from the fragments in bottom of the reagent containers. The floaters were from the other slides in the specific rack stained. The key finding was that BLANK slides put through a stainer at the end of the day (after staining over 1300 slides and accumulating the maximum amount of tissue fragments in the reagent baths) DID NOT PICK UP ANY FLOATERS. ALL the floaters that they determined had come from the staining process occurred ONLY when tissue slides were included in the stain rack (They alternated blank and tissue slides in the rack). So, the tissue seen in the stain dish does not appear to contribute to floaters. A stain instrument vendor repeated this experiment in our lab and came up with the same result. Our pathologists were mixed on the floater question. Some felt a system that eliminated floaters would be beneficial. Others thought floaters were easily detected and the vast majority could be ignored. It remains to be determined it is cost effective to replace the current system with one that would eliminate floaters. It does not appear to be a sole reason to replace the current system. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 06, 2011 6:34 AM To: histonet@lists.utsouthwestern.edu; Janice Mahoney Subject: [Histonet] Floaters This is a very interesting subject indeed. To start I always take "with a grain of salt" whatever a vendor tells me because their opinions may (or may not, but just in case) biased to the products they sell. The same thing goes for any automobiles salesperson of any automobile make claiming the "wonders" of the make they sell compared with other makes. Now, to the floater. I have also seen pieces of sections on the bottom of staining dishes. They are there because they fell-off the stained section. My question is: what are the possibilities of that piece of lost stained section that fell from its original slide, "re-float" and magically adhere to another slide? I really think that the possibilities range from almost none, to none. Going to the water bath: yes, this is a major source of floaters because a piece of a section left in the water bath can be picked up in another slide along with a section from another case. That is why it is mandatory to swipe the surface of the water bath with? a tissue after each case is cut. The source can be defined if we keep a record of the sequence in which the blocks are cut (as was standard procedure at my lab). Whenever we ended with a floater in a section we went immediately to the block to determine if the floater came from the block or the water bath. The other sources, either during description, cassetting or embedding are going to end in the block and it is very difficult to determine the origin. The only thing to do is to make sure that embedding wells where the forceps rest and heat, are thoroughly cleaned when the embedding ends. But I think that the main thrust of the question deals with floaters coming from the staining dishes: as I wrote earlier I give to this source?a very slim and almost impossible chance. Ren? J. ? ? ? --- On Wed, 10/5/11, Janice Mahoney wrote: From: Janice Mahoney Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 5, 2011, 8:11 PM Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets.? There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue.? The answers were surprisingly pretty much the same.???It is not an issue!? Now I understand how one company can make this claim as their stainer uses fresh stain on each slide.? The explanations from the other companies were insulting and just plain did not make sense to me.? I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath."? Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places.? I have seen them from the doctor's office or procedure room to the stainer and every step in between.? Sometimes if the "floater" is in the block it is very difficult to determine where it originated.? We can however eliminate the water bath and stainer as the origin in these cases.? One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle.? I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes.? So, no, not all floaters float.? I would love to hear feedback from others on this.? I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it.? I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mamawooo <@t> hotmail.com Thu Oct 6 10:23:53 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Thu Oct 6 10:23:58 2011 Subject: [Histonet] (no subject) In-Reply-To: <4E8D6628.D67D.00AF.1@mercyhealth.com> References: , , <4E8D6628.D67D.00AF.1@mercyhealth.com> Message-ID: Very good question Cindi.Jan > Date: Thu, 6 Oct 2011 09:26:18 -0400 > From: robinsoc@mercyhealth.com > To: mamawooo@hotmail.com; histonet@lists.utsouthwestern.edu; christiegowan@msn.com > Subject: RE: [Histonet] (no subject) > > It is a good thing this vendor does not work in a histo lab because with that comment/attitude he/she would not last long. I have heard the water bath theory but upon investigation this has never been the source because every histotech I work with cleans it each and every time. We have traced floaters back to grossing stations, processors...especially placenta getting 'snagged' on a piece of bone...embedding forceps (we now use ones with no grooves), forcep warmers, stains and coverslippers. We have significantly reduced the number we see since we make the effort to track each one found as an ongoing QA project, but we still see the occasional floater. We did find that we have to put tissue types which fragment easily into mesh cassettes or bags but this can cause issues with fluid exchange and carryover during processing. It is a balancing act. I have always wondering about the newer processors with orientation cassettes which are embed and then cut without opening. Do they see less floaters with this type of 'closed' system? > > > > Cindi Robinson HT(ASCP) > Mercy Medical Center > Dunes Medical Laboratories > 350 W Anchor Dr > Dakota Dunes SD 57049 > phone-712-279-2768 > robinsoc@mercyhealth.com > > > >>> CHRISTIE GOWAN 10/6/2011 7:44 AM >>> > > I agree with you Jan. In my 30 + years as a histotech (yes, I am old too) I have seen floaters come from a variety of places but I am hard pressed to remember any floater coming from the water bath. Today we are blessed with DNA fingerprinting to determine if the floater is or is not from the patient but that still does not address the real issue of where did it come from and how do we stop it. The vendors stating that it is not an issue have never been re-biopsied because of a floater in with their tissue. Good discussion and long overdue. I look forward to the day when it is no longer an automatic response from all involved that it is "Histology's fault". Hope you are enjoying your new adventures in retirement. See you next year in Canada! > > > > From: mamawooo@hotmail.com > > To: histonet@lists.utsouthwestern.edu > > Date: Wed, 5 Oct 2011 14:11:25 -1000 > > Subject: [Histonet] (no subject) > > > > > > Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets. There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue. The answers were surprisingly pretty much the same. It is not an issue! Now I understand how one company can make this claim as their stainer uses fresh stain on each slide. The explanations from the other companies were insulting and just plain did not make sense to me. I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath." Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places. I have seen them from the doctor's office or procedure room to the stainer and every step in between. Sometimes if the "floater" is in the block it is very difficult to determine where it originated. We can however eliminate the water bath and stainer as the origin in these cases. One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle. I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes. So, no, not all floaters float. I would love to hear feedback from others on this. I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it. I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Oct 6 10:37:57 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Oct 6 10:38:02 2011 Subject: [Histonet] "formol" In-Reply-To: <1317906775.25229.YahooMailClassic@web65703.mail.ac4.yahoo.com> References: <1317906775.25229.YahooMailClassic@web65703.mail.ac4.yahoo.com> Message-ID: <4C8F235CA0384093830B90BB552641EA@dielangs.at> Formol is an old brandname, I think for a desinfection reagent. Not really custom any more. Bye (another) Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rene J Buesa Gesendet: Donnerstag, 06. Oktober 2011 15:13 An: histonet@lists.utsouthwestern.edu; Jenny Vega Betreff: [Histonet] Difference between Neutral Buffered Formalin 10% and NBformaldehyde 3.7 to 4% Jenny: Formaldehyde is?the GAS methanal?that dissolves in water at a maximum concentration between 37-40% vol./vol. To prevent its polymerization, small amounts of mathanol (an alcohol)are added. The 10% solution formaldehyde contains between 3.7 and 4.0% of formaldehyde and is called "formalin" in many countries and "formol" in Europe. So formalin is a 3.7 to 4.0% solution of formaldehyde. So a 10% formalin = 3.7-4.0% formaldehyde. NBF is prepared by added phosphate salts to prevent the formalin to acidify and is used to fix tissues. It is a pitty that your supervisor has so little knowledge about the area she is supposed to supervise, but I am not do not know why, but I am not.?surprised. To your question: SHE is wrong, really wrong! Ren? J. ? --- On Wed, 10/5/11, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 5, 2011, 7:03 PM Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it? we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to? putrefied if they are put in that solution. Am I wrong or is she wrong? thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KCross <@t> cvm.tamu.edu Thu Oct 6 10:43:15 2011 From: KCross <@t> cvm.tamu.edu (Kelly Cross) Date: Thu Oct 6 10:43:22 2011 Subject: [Histonet] control for Hemoglobin? Message-ID: <869848DDBB7C5D4896A569A38B814E690551CAD1@CVMMB01.cvm.tamu.edu> Hi Histonetters, What control should I use for Okajima's Stain for hemoglobin? Thank you for your help! Kelly Cross B.S., HT (ASCP) Medical Laboratory Supervisor Texas A&M University VTPB Histology Lab VMA bldg. 214 College Station, TX 77843-4467 Office: 979-862-3658 Lab: 979-845-5149 From mamawooo <@t> hotmail.com Thu Oct 6 10:44:31 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Thu Oct 6 10:44:35 2011 Subject: [Histonet] Tissue left in processor In-Reply-To: <1317910434.70026.YahooMailClassic@web65710.mail.ac4.yahoo.com> References: <3809C163DC1DA54AA534B3C7794D07B68EC20DC73B@ENHBGMBX01.PA.LCL>, <1317910434.70026.YahooMailClassic@web65710.mail.ac4.yahoo.com> Message-ID: I agree with Rene, as long as the temp is only a few degrees above the melting point of the paraffin.Jan,Omaha > Date: Thu, 6 Oct 2011 07:13:54 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; rcharles@pa.gov > Subject: Re: [Histonet] Tissue left in processor > CC: > > I do not think that a well fixed, well processed tissue left in molten paraffin for 2 hours after the processor finished will have any adverse outcome. > Ren? J. > > --- On Thu, 10/6/11, Charles, Roger wrote: > > > From: Charles, Roger > Subject: [Histonet] Tissue left in processor > To: "Histonet (histonet@lists.utsouthwestern.edu)" > Date: Thursday, October 6, 2011, 10:05 AM > > > Hi All, > Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes? And if so what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing was completed? > Thanks so much. > Roger > > Roger Charles| Microbiologist II > Pennsylvania Veterinary Laboratory > 2305 North Cameron Street | Harrisburg, PA 17110 > Phone: 717.787.8808 | Fax: 717.772.3895 > www.agriculture.state.pa.us > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tammy.Pickles <@t> inspection.gc.ca Thu Oct 6 10:49:08 2011 From: Tammy.Pickles <@t> inspection.gc.ca (Tammy Pickles) Date: Thu Oct 6 10:49:17 2011 Subject: [Histonet] Imidazole buffer Message-ID: <4E8D7994.B440.007F.1@inspection.gc.ca> Hi, I am wondering if anyone out there can tell me the function of Imidazole, when used in a buffer. We are currently using it to make up our in house DAB solution. The results are fantastic with an overall increase in sensitivity with all of our antibodies. The only information I can find concerning the function is to help in blocking nonspecific staining. Does anyone have more information to share? Thanks in advance, Tammy From gayle.callis <@t> bresnan.net Thu Oct 6 10:59:32 2011 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Oct 6 10:59:53 2011 Subject: [Histonet] RE: DAPI on thicker sections, problem with gradient Message-ID: <000601cc8440$f1af4270$d50dc750$@bresnan.net> Carmen, You wrote: Good morning: I am Carmen Garcia from the Faculty of Medicine of the University of Valencia, Spain. I usually use your vectashield mounting medium for fluorescence with DAPI (H-1200) and I never have any problem. But now I am working with 50um frozen sections and the problem that I have is that the DAPI staining does not arrive to the bottom of the section. Do you have any sugesstion of what can I do? (I already have the sections permeabilized with triton x-100) I have thougth to incubate with the mounting medium some hours at 4?C or put some mounting medium in the antibody diluent and incubate... any suggestion would be really welcome. Thank you so much, Kind regards, Carmen **************************************************************************** *********** The problem you are experiencing is what we refer to as a DAPI staining gradient. Uneven staining occurs with the DAPI is not reaching all the cells due to viscosity of the mounting media and thickness of the tissue section. You did not say whether your thick sections have been fixed before sectioning and staining for a tissue component, and then the DAPI - please explain. I suggest you do NOT use a mounting media with DAPI, but rather make a DAPI solution which you can then apply to the section and incubate mounting the coverslip with Vectashield that does not contain DAPI. Using a DAPI solution may shorten the time you now use, e.g. "some hours". You can buy the DAPI, ready to use from Biogenex or other vendors, but making it in house is simple. I would assume you could add Triton X to the DAPI solution to improve permeability and match what you have already done with that detergent. After DAPI staining, mount the coverslip using VectaShield without DAPI. DAPI recipes can be found on the internet, just Google DAPI. I found it on a Johns Hopkins webpage, but IHC world may have it too. There are other sources of ready to use DAPI if you look. We have used Prolong Gold antifade reagent with DAPI, and get a staining gradient on 5 um sections. This is very annoying, and probably caused by the actual technique of cover slipping itself. We are now going to use Biogenex ready to use DAPI before the Prolong Gold antifade reagent without DAPUI. Staining for a thin section takes 5 mintues at RT, so you should try several 50 um sections (non experimental) to determine your optimal staining time. It may take only 15 minutes although cold, 4C temperature may slow down the penetration of the stain through the thicker section. This way you can achieve even staining of your nuclei without long incubations in the more viscious mounting media containing DAPI. Good luck, and let us know if you have success................... Gayle M. Callis HTL/HT/MT(ASCP) From hfedor <@t> jhmi.edu Thu Oct 6 11:06:06 2011 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Oct 6 11:06:31 2011 Subject: [Histonet] RE: DAPI on thicker sections, problem with gradient In-Reply-To: <000601cc8440$f1af4270$d50dc750$@bresnan.net> References: <000601cc8440$f1af4270$d50dc750$@bresnan.net> Message-ID: Hello, Maybe this method was meant to be used on Floating sections instead of mounted sections. That way the binding and penetration would go from both sides of the tissue. Helen L. Fedor http://tmalab.jhmi.edu/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Thursday, October 06, 2011 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: DAPI on thicker sections, problem with gradient Carmen, You wrote: Good morning: I am Carmen Garcia from the Faculty of Medicine of the University of Valencia, Spain. I usually use your vectashield mounting medium for fluorescence with DAPI (H-1200) and I never have any problem. But now I am working with 50um frozen sections and the problem that I have is that the DAPI staining does not arrive to the bottom of the section. Do you have any sugesstion of what can I do? (I already have the sections permeabilized with triton x-100) I have thougth to incubate with the mounting medium some hours at 4?C or put some mounting medium in the antibody diluent and incubate... any suggestion would be really welcome. Thank you so much, Kind regards, Carmen **************************************************************************** *********** The problem you are experiencing is what we refer to as a DAPI staining gradient. Uneven staining occurs with the DAPI is not reaching all the cells due to viscosity of the mounting media and thickness of the tissue section. You did not say whether your thick sections have been fixed before sectioning and staining for a tissue component, and then the DAPI - please explain. I suggest you do NOT use a mounting media with DAPI, but rather make a DAPI solution which you can then apply to the section and incubate mounting the coverslip with Vectashield that does not contain DAPI. Using a DAPI solution may shorten the time you now use, e.g. "some hours". You can buy the DAPI, ready to use from Biogenex or other vendors, but making it in house is simple. I would assume you could add Triton X to the DAPI solution to improve permeability and match what you have already done with that detergent. After DAPI staining, mount the coverslip using VectaShield without DAPI. DAPI recipes can be found on the internet, just Google DAPI. I found it on a Johns Hopkins webpage, but IHC world may have it too. There are other sources of ready to use DAPI if you look. We have used Prolong Gold antifade reagent with DAPI, and get a staining gradient on 5 um sections. This is very annoying, and probably caused by the actual technique of cover slipping itself. We are now going to use Biogenex ready to use DAPI before the Prolong Gold antifade reagent without DAPUI. Staining for a thin section takes 5 mintues at RT, so you should try several 50 um sections (non experimental) to determine your optimal staining time. It may take only 15 minutes although cold, 4C temperature may slow down the penetration of the stain through the thicker section. This way you can achieve even staining of your nuclei without long incubations in the more viscious mounting media containing DAPI. Good luck, and let us know if you have success................... Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From idimitro <@t> mun.ca Thu Oct 6 11:09:28 2011 From: idimitro <@t> mun.ca (idimitro@mun.ca) Date: Thu Oct 6 11:09:38 2011 Subject: [Histonet] RE: Cutting frozen sections from visceral fat In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D50BB4C@SN2PRD0702MB110.namprd07.prod.outlook.com> References: <14C3108E8B98EF43B3EDAE5833670034050FD5@exchange.med.mun.ca> <8A70A9B2ECDD084DACFE6C59FCF86D50BB4C@SN2PRD0702MB110.namprd07.prod.outlook.com> Message-ID: <14C3108E8B98EF43B3EDAE58336700340511A2@exchange.med.mun.ca> Hi Sarah, Thank you for the response. We are a research lab so whatever the researchers want to be done we do it with all kinds of tissues. In this case they are looking at the size of the fat cells in the animals. I will try to lower the temperature to -30 as was also suggested to me and if this does not work I will look into fixation. In Bancroft's fifth edition is mentioned that if you need fixation you need to use formol-calcium. I hope this will harden the fat and stabilize the membrane proteins enough so I can cut it. I will let you know how it worked. Thanks again, iliana -----Original Message----- From: Sarah Dysart [mailto:sdysart@mirnarx.com] Sent: October 6, 2011 12:14 PM To: Dimitrova, Iliana Subject: RE: Cutting frozen sections from visceral fat Cutting frozen fat is basically impossible. It will just goo up and rip out of the chuck of frozen OCT. I think there is a way where you can do a fixation process first in something, but I'm not sure of that. Why do they need frozen fat sections? Cutting fat after fixation is totally doable with the right fixation. I have done this when people have asked for frozen fat sections. I can usually talk them out of the frozen and just do the FFPE sections. Let me know I can give you more details. http://www.pathologyinnovations.com/new_page_2.htm This site has a section in it called wrestling the fat one, that has some ideas for you. Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of idimitro@mun.ca Sent: Thursday, October 06, 2011 6:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting frozen sections from visceral fat Hi everyone, I am having trouble cutting frozen sections from visceral fat. I have never cut only fat before. I am also cutting liver sections and they are perfect, but when I try to cut the fat it is not working. The temperature of the cryostat is ( -20) degree Celsius. I wonder if anyone has ever done this. I was thinking that if I lower the temperature or fix the fat with formol-calcium I may have success. Iliana Dimitrova, MSc, RT Histology Supervisor Room 2808 Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University St. John's, NL This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php From mjdessoye <@t> wvhcs.org Thu Oct 6 11:30:02 2011 From: mjdessoye <@t> wvhcs.org (Dessoye, Michael J) Date: Thu Oct 6 11:30:09 2011 Subject: [Histonet] CMV/HSV Protocols Message-ID: Does anyone have protocols for CMV, HSV I, and HSV II (from Cell Marque) that they would be willing to share for the Benchmark Ultra (i-View)? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From joelleweaver <@t> hotmail.com Thu Oct 6 11:39:38 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Oct 6 11:39:43 2011 Subject: [Histonet] Floaters In-Reply-To: <8D7C2D242DBD45498006B21122072BF89448A27E@MCINFRWEM003.ucsfmedicalcenter.org> References: , <1317908024.35201.YahooMailClassic@web65703.mail.ac4.yahoo.com>, <8D7C2D242DBD45498006B21122072BF89448A27E@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: This was an excellent article, I archived it, interesting that your own experiments confirmed this results. Joelle Weaver MAOM, BA, (HTL) ASCP > From: Timothy.Morken@ucsfmedctr.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 6 Oct 2011 08:19:25 -0700 > Subject: RE: [Histonet] Floaters > > The recent paper by Platt et all (Tissue Floaters and Contaminants in the Histology Laboratory, Arch Pathol Lab Med, Vol 133, June 2009) details floaters from waterbaths and staining process pretty well. > > Waterbaths do not appear to be a contributor. They literally found only ONE tissue fragment in waterbath water saved from an entire day of sectioning from 13 waterbaths. And it was in the water, not on a slide. They apparently did not identify ANY waterbath-derived floaters on any slide. They attribute this finding to the fact the tissue is still in paraffin so the tissue was either on the slide or stuck to the side of the waterbath, not floating in the water. > > To determine the extent of staining-derived floaters they measured the amount of tissue fragments found in each staining station. The vast majority were in the first xylene and alcohol baths. After the 95% alcohol there were only very rare tissue fragments in the rest of the baths. > > They found the staining process does contribute floaters (besides those that are clearly in the block), but apparently the do not derive from the fragments in bottom of the reagent containers. The floaters were from the other slides in the specific rack stained. The key finding was that BLANK slides put through a stainer at the end of the day (after staining over 1300 slides and accumulating the maximum amount of tissue fragments in the reagent baths) DID NOT PICK UP ANY FLOATERS. > > ALL the floaters that they determined had come from the staining process occurred ONLY when tissue slides were included in the stain rack (They alternated blank and tissue slides in the rack). So, the tissue seen in the stain dish does not appear to contribute to floaters. > > A stain instrument vendor repeated this experiment in our lab and came up with the same result. > > Our pathologists were mixed on the floater question. Some felt a system that eliminated floaters would be beneficial. Others thought floaters were easily detected and the vast majority could be ignored. It remains to be determined it is cost effective to replace the current system with one that would eliminate floaters. It does not appear to be a sole reason to replace the current system. > > Tim Morken > Supervisor, Histology, IPOX > UCSF Medical Center > San Francisco, CA, USA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, October 06, 2011 6:34 AM > To: histonet@lists.utsouthwestern.edu; Janice Mahoney > Subject: [Histonet] Floaters > > This is a very interesting subject indeed. To start I always take "with a grain of salt" whatever a vendor tells me because their opinions may (or may not, but just in case) biased to the products they sell. The same thing goes for any automobiles salesperson of any automobile make claiming the "wonders" of the make they sell compared with other makes. > Now, to the floater. I have also seen pieces of sections on the bottom of staining dishes. They are there because they fell-off the stained section. My question is: what are the possibilities of that piece of lost stained section that fell from its original slide, "re-float" and magically adhere to another slide? I really think that the possibilities range from almost none, to none. > Going to the water bath: yes, this is a major source of floaters because a piece of a section left in the water bath can be picked up in another slide along with a section from another case. That is why it is mandatory to swipe the surface of the water bath with a tissue after each case is cut. The source can be defined if we keep a record of the sequence in which the blocks are cut (as was standard procedure at my lab). > Whenever we ended with a floater in a section we went immediately to the block to determine if the floater came from the block or the water bath. > The other sources, either during description, cassetting or embedding are going to end in the block and it is very difficult to determine the origin. The only thing to do is to make sure that embedding wells where the forceps rest and heat, are thoroughly cleaned when the embedding ends. > But I think that the main thrust of the question deals with floaters coming from the staining dishes: as I wrote earlier I give to this source a very slim and almost impossible chance. > Ren? J. > > > > > --- On Wed, 10/5/11, Janice Mahoney wrote: > > > From: Janice Mahoney > Subject: [Histonet] (no subject) > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, October 5, 2011, 8:11 PM > > > > Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets. There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue. The answers were surprisingly pretty much the same. It is not an issue! Now I understand how one company can make this claim as their stainer uses fresh stain on each slide. The explanations from the other companies were insulting and just plain did not make sense to me. I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath." Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places. I have seen them from the doctor's office or procedure room to > the stainer and every step in between. Sometimes if the "floater" is in the block it is very difficult to determine where it originated. We can however eliminate the water bath and stainer as the origin in these cases. One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle. I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes. So, no, not all floaters float. I would love to hear feedback from others on this. I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it. I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Oct 6 11:55:09 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Oct 6 11:55:14 2011 Subject: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DFB21@nmdamailsvr.nmda.ad.nmsu.edu> References: <1317906775.25229.YahooMailClassic@web65703.mail.ac4.yahoo.com>, , <4D14F0FC9316DD41972D5F03C070908B051DFB21@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: I like this article's discussion of the history of usuage and terminology: citation Fox, C., Johnson, F., Whiting, J., and Roller, P. Formaldehyde Fixation, The Journal of Histochemistry and Cytochemistry, Vol. 33, No.8, pp. 845-853, 1985. Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Thu, 6 Oct 2011 08:56:55 -0600 > From: sbreeden@nmda.nmsu.edu > To: Gudrun.Erikstad@stolav.no; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% > CC: > > Now, wait just a minute! Don't tell me that after 40+ years of being a > histotech that I've been WRONG all this time??? FORMALDEHYDE is > FORMALDEHYDE is FORMALDEHYDE. FORMALIN is the diluted form of > FORMALDEHYDE, buffered and used at (usually) 10%. If I'm wrong, this > will be my last day at work... :) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Oct 6 11:55:11 2011 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Oct 6 11:55:31 2011 Subject: [Histonet] Tissue left in processor In-Reply-To: References: <3809C163DC1DA54AA534B3C7794D07B68EC20DC73B@ENHBGMBX01.PA.LCL>, <1317910434.70026.YahooMailClassic@web65710.mail.ac4.yahoo.com> Message-ID: <000001cc8448$b806d8f0$28148ad0$@bresnan.net> Some prefer to take the cassettes off the processor and let them harden until embedding can be done, rather than leave in molten, hot paraffin. This does not damage the tissue, and placing the cooled cassettes into the embedding center holding area allows the paraffin to re-melt in a shorter time before embedding. This has been discussed in the past on Histonet. Some tissues may dry out even more WITH difficult sectioning after prolonged heat exposure. One result will be a parched earth effect seen in sections. I see you are from a veterinary facility, and if you work with rodent tissues e.g. spleen and liver, you should not let the tissue sit in paraffin or you will have little hard rocks to section. If your sectioning suffers (dry, friable, shattered, hard) after allowing tissues to sit in hot paraffin as compared to the times when you embed asap after processing is finished, then over exposure to hot paraffin can contributing factor. I personally do not like to "cook" my tissue any longer than necessary and heat labile antigens will also be at risk. I schedule so I can be there to embed when the processing is completed. Our standard is to embed when processing is finished and schedule accordingly. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Thursday, October 06, 2011 9:45 AM To: rjbuesa@yahoo.com; histo net; rcharles@pa.gov Subject: RE: [Histonet] Tissue left in processor I agree with Rene, as long as the temp is only a few degrees above the melting point of the paraffin.Jan,Omaha > Date: Thu, 6 Oct 2011 07:13:54 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; rcharles@pa.gov > Subject: Re: [Histonet] Tissue left in processor > CC: > > I do not think that a well fixed, well processed tissue left in molten paraffin for 2 hours after the processor finished will have any adverse outcome. > Ren? J. > > --- On Thu, 10/6/11, Charles, Roger wrote: > > > From: Charles, Roger > Subject: [Histonet] Tissue left in processor > To: "Histonet (histonet@lists.utsouthwestern.edu)" > > Date: Thursday, October 6, 2011, 10:05 AM > > > Hi All, > Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes? And if so what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing was completed? > Thanks so much. > Roger > > Roger Charles| Microbiologist II > Pennsylvania Veterinary Laboratory > 2305 North Cameron Street | Harrisburg, PA 17110 > Phone: 717.787.8808 | Fax: 717.772.3895 > www.agriculture.state.pa.us From af46 <@t> buffalo.edu Thu Oct 6 12:15:07 2011 From: af46 <@t> buffalo.edu (Featherstone, Annette) Date: Thu Oct 6 12:15:22 2011 Subject: [Histonet] processor options? Message-ID: Maybe someone can come up with a tissue processor that will have an option that will drain the paraffin after a time limit and let tissue cassettes harden in a cooled retort. This would be invaluable to all conscientious histotechs who have tried to make it in to embed when the weather has shut down roads for extended periods of time. Or if you are a "lone" technician who has a family emergency and cannot get in to take the tissue off the machine. How about it, Leica, Sakura, Thermo? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, October 06, 2011 12:58 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 95, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Tissue left in processor (Rene J Buesa) 2. RE: Tissue left in processor (Sherwood, Margaret) 3. SV: [Histonet] Difference between Neutral Buffered Formalin 10% and NBformaldehyde 3.7 to 4% (Erikstad, Gudrun Hovstein) 4. Re: (no subject) (Angela Bitting) 5. RE: Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% (Breeden, Sara) 6. Ken Marissael is out of the office (Ken_Marissael@vwr.com) 7. RE: Floaters (Morken, Timothy) 8. RE: (no subject) (Janice Mahoney) 9. "formol" (Gudrun Lang) 10. control for Hemoglobin? (Kelly Cross) 11. RE: Tissue left in processor (Janice Mahoney) 12. Imidazole buffer (Tammy Pickles) 13. RE: DAPI on thicker sections, problem with gradient (gayle callis) 14. RE: RE: DAPI on thicker sections, problem with gradient (Helen Fedor) 15. RE: Cutting frozen sections from visceral fat (idimitro@mun.ca) 16. CMV/HSV Protocols (Dessoye, Michael J) 17. RE: Floaters (joelle weaver) 18. RE: Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% (joelle weaver) 19. RE: Tissue left in processor (gayle callis) ---------------------------------------------------------------------- Message: 1 Date: Thu, 6 Oct 2011 07:13:54 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Tissue left in processor To: "Histonet \(histonet@lists.utsouthwestern.edu\)" , RogerCharles Message-ID: <1317910434.70026.YahooMailClassic@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I do not think that a well fixed, well processed tissue left in molten paraffin for 2 hours after the processor finished will have any adverse outcome. Ren? J. --- On Thu, 10/6/11, Charles, Roger wrote: From: Charles, Roger Subject: [Histonet] Tissue left in processor To: "Histonet (histonet@lists.utsouthwestern.edu)" Date: Thursday, October 6, 2011, 10:05 AM Hi All, Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes?? And if so? what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing was completed? Thanks so much. Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 6 Oct 2011 10:17:43 -0400 From: "Sherwood, Margaret" Subject: RE: [Histonet] Tissue left in processor To: "Charles, Roger" , Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A8C@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" I don't think you want to leave it in the processor for 2 hours. Even at the embedding center, if someone can't come to help orient the tissue, we then take the cassettes out of the paraffin station and leave on a paper towel. Have done that for sometimes 2-3 days. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Thursday, October 06, 2011 10:05 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Tissue left in processor Hi All, Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes? And if so what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing was completed? Thanks so much. Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 3 Date: Thu, 6 Oct 2011 16:22:55 +0200 From: "Erikstad, Gudrun Hovstein" Subject: SV: [Histonet] Difference between Neutral Buffered Formalin 10% and NBformaldehyde 3.7 to 4% To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I suppose Ren? meant to say: Formalin is a 37-40% solution of formaldehyde. 10% formalin is a 3,7-4,0% solution of formaldehyde. I've never heard the use of "Formol", but then again, I'm scandinavian, not som much european... ;-) Gudrun, Norway -----Opprinnelig melding----- Fra: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] P? vegne av Rene J Buesa Sendt: 6. oktober 2011 15:13 Til: histonet@lists.utsouthwestern.edu; Jenny Vega Emne: [Histonet] Difference between Neutral Buffered Formalin 10% and NBformaldehyde 3.7 to 4% Jenny: Formaldehyde is?the GAS methanal?that dissolves in water at a maximum concentration between 37-40% vol./vol. To prevent its polymerization, small amounts of mathanol (an alcohol)are added. The 10% solution formaldehyde contains between 3.7 and 4.0% of formaldehyde and is called "formalin" in many countries and "formol" in Europe. So formalin is a 3.7 to 4.0% solution of formaldehyde. So a 10% formalin = 3.7-4.0% formaldehyde. NBF is prepared by added phosphate salts to prevent the formalin to acidify and is used to fix tissues. It is a pitty that your supervisor has so little knowledge about the area she is supposed to supervise, but I am not do not know why, but I am not.?surprised. To your question: SHE is wrong, really wrong! Ren? J. ? --- On Wed, 10/5/11, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 5, 2011, 7:03 PM Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it? we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to? putrefied if they are put in that solution. Am I wrong or is she wrong? thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 06 Oct 2011 10:31:53 -0400 From: "Angela Bitting" Subject: Re: [Histonet] (no subject) To: "Janice Mahoney" , Message-ID: <4E8D8398.2B7F.00C9.1@geisinger.edu> Content-Type: text/plain; charset="us-ascii" I too spoke to one vendor who claimed that the floaters are not an issue and that their bottles drew below the level of the "floating" floaters. I didn't feel insulted, but did feel that this salesperson obviously had no actual lab experience or they never would have made such a ridiculous claim. I always put the opinions and experiences of other Histotechs far ahead of anything I hear from sales reps. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 >>> Janice Mahoney 10/5/2011 8:11 PM >>> Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets. There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue. The answers were surprisingly pretty much the same. It is not an issue! Now I understand how one company can make this claim as their stainer uses fresh stain on each slide. The explanations from the other companies were insulting and just plain did not make sense to me. I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath." Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places. I have seen them from the doctor's office or procedure room to the stainer and every step in be tween. Sometimes if the "floater" is in the block it is very difficult to determine where it originated. We can however eliminate the water bath and stainer as the origin in these cases. One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle. I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes. So, no, not all floaters float. I would love to hear feedback from others on this. I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it. I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 5 Date: Thu, 6 Oct 2011 08:56:55 -0600 From: "Breeden, Sara" Subject: RE: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% To: "Erikstad, Gudrun Hovstein" , Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFB21@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Now, wait just a minute! Don't tell me that after 40+ years of being a histotech that I've been WRONG all this time??? FORMALDEHYDE is FORMALDEHYDE is FORMALDEHYDE. FORMALIN is the diluted form of FORMALDEHYDE, buffered and used at (usually) 10%. If I'm wrong, this will be my last day at work... :) ------------------------------ Message: 6 Date: Thu, 6 Oct 2011 11:01:12 -0400 From: Ken_Marissael@vwr.com Subject: [Histonet] Ken Marissael is out of the office To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 10/06/2011 and will not return until 10/12/2011. I will be away on 10/06 and return 10/12. I will have limited e-mail and cell phone access, but will try to get back to you as quickly as possible. While I am away, please contact VWR Healthcare Customer Service at 877-881-1192. If you require immediate attenion, please contact Jackie Zerillo at 609-410-6152. She is the NYC rep. ------------------------------ Message: 7 Date: Thu, 6 Oct 2011 08:19:25 -0700 From: "Morken, Timothy" Subject: RE: [Histonet] Floaters To: "histonet@lists.utsouthwestern.edu" Message-ID: <8D7C2D242DBD45498006B21122072BF89448A27E@MCINFRWEM003.ucsfmedicalcenter.org> Content-Type: text/plain; charset=iso-8859-1 The recent paper by Platt et all (Tissue Floaters and Contaminants in the Histology Laboratory, Arch Pathol Lab Med, Vol 133, June 2009) details floaters from waterbaths and staining process pretty well. Waterbaths do not appear to be a contributor. They literally found only ONE tissue fragment in waterbath water saved from an entire day of sectioning from 13 waterbaths. And it was in the water, not on a slide. They apparently did not identify ANY waterbath-derived floaters on any slide. They attribute this finding to the fact the tissue is still in paraffin so the tissue was either on the slide or stuck to the side of the waterbath, not floating in the water. To determine the extent of staining-derived floaters they measured the amount of tissue fragments found in each staining station. The vast majority were in the first xylene and alcohol baths. After the 95% alcohol there were only very rare tissue fragments in the rest of the baths. They found the staining process does contribute floaters (besides those that are clearly in the block), but apparently the do not derive from the fragments in bottom of the reagent containers. The floaters were from the other slides in the specific rack stained. The key finding was that BLANK slides put through a stainer at the end of the day (after staining over 1300 slides and accumulating the maximum amount of tissue fragments in the reagent baths) DID NOT PICK UP ANY FLOATERS. ALL the floaters that they determined had come from the staining process occurred ONLY when tissue slides were included in the stain rack (They alternated blank and tissue slides in the rack). So, the tissue seen in the stain dish does not appear to contribute to floaters. A stain instrument vendor repeated this experiment in our lab and came up with the same result. Our pathologists were mixed on the floater question. Some felt a system that eliminated floaters would be beneficial. Others thought floaters were easily detected and the vast majority could be ignored. It remains to be determined it is cost effective to replace the current system with one that would eliminate floaters. It does not appear to be a sole reason to replace the current system. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 06, 2011 6:34 AM To: histonet@lists.utsouthwestern.edu; Janice Mahoney Subject: [Histonet] Floaters This is a very interesting subject indeed. To start I always take "with a grain of salt" whatever a vendor tells me because their opinions may (or may not, but just in case) biased to the products they sell. The same thing goes for any automobiles salesperson of any automobile make claiming the "wonders" of the make they sell compared with other makes. Now, to the floater. I have also seen pieces of sections on the bottom of staining dishes. They are there because they fell-off the stained section. My question is: what are the possibilities of that piece of lost stained section that fell from its original slide, "re-float" and magically adhere to another slide? I really think that the possibilities range from almost none, to none. Going to the water bath: yes, this is a major source of floaters because a piece of a section left in the water bath can be picked up in another slide along with a section from another case. That is why it is mandatory to swipe the surface of the water bath with? a tissue after each case is cut. The source can be defined if we keep a record of the sequence in which the blocks are cut (as was standard procedure at my lab). Whenever we ended with a floater in a section we went immediately to the block to determine if the floater came from the block or the water bath. The other sources, either during description, cassetting or embedding are going to end in the block and it is very difficult to determine the origin. The only thing to do is to make sure that embedding wells where the forceps rest and heat, are thoroughly cleaned when the embedding ends. But I think that the main thrust of the question deals with floaters coming from the staining dishes: as I wrote earlier I give to this source?a very slim and almost impossible chance. Ren? J. ? ? ? --- On Wed, 10/5/11, Janice Mahoney wrote: From: Janice Mahoney Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 5, 2011, 8:11 PM Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets.? There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue.? The answers were surprisingly pretty much the same.???It is not an issue!? Now I understand how one company can make this claim as their stainer uses fresh stain on each slide.? The explanations from the other companies were insulting and just plain did not make sense to me.? I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath."? Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places.? I have seen them from the doctor's office or procedure room to the stainer and every step in between.? Sometimes if the "floater" is in the block it is very difficult to determine where it originated.? We can however eliminate the water bath and stainer as the origin in these cases.? One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle.? I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes.? So, no, not all floaters float.? I would love to hear feedback from others on this.? I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it.? I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 6 Oct 2011 05:23:53 -1000 From: Janice Mahoney Subject: RE: [Histonet] (no subject) To: Cindy Robinson , histo net , Christie Gowan Message-ID: Content-Type: text/plain; charset="iso-8859-1" Very good question Cindi.Jan > Date: Thu, 6 Oct 2011 09:26:18 -0400 > From: robinsoc@mercyhealth.com > To: mamawooo@hotmail.com; histonet@lists.utsouthwestern.edu; christiegowan@msn.com > Subject: RE: [Histonet] (no subject) > > It is a good thing this vendor does not work in a histo lab because with that comment/attitude he/she would not last long. I have heard the water bath theory but upon investigation this has never been the source because every histotech I work with cleans it each and every time. We have traced floaters back to grossing stations, processors...especially placenta getting 'snagged' on a piece of bone...embedding forceps (we now use ones with no grooves), forcep warmers, stains and coverslippers. We have significantly reduced the number we see since we make the effort to track each one found as an ongoing QA project, but we still see the occasional floater. We did find that we have to put tissue types which fragment easily into mesh cassettes or bags but this can cause issues with fluid exchange and carryover during processing. It is a balancing act. I have always wondering about the newer processors with orientation cassettes which are embed and then cut without opening. Do th ey see less floaters with this type of 'closed' system? > > > > Cindi Robinson HT(ASCP) > Mercy Medical Center > Dunes Medical Laboratories > 350 W Anchor Dr > Dakota Dunes SD 57049 > phone-712-279-2768 > robinsoc@mercyhealth.com > > > >>> CHRISTIE GOWAN 10/6/2011 7:44 AM >>> > > I agree with you Jan. In my 30 + years as a histotech (yes, I am old too) I have seen floaters come from a variety of places but I am hard pressed to remember any floater coming from the water bath. Today we are blessed with DNA fingerprinting to determine if the floater is or is not from the patient but that still does not address the real issue of where did it come from and how do we stop it. The vendors stating that it is not an issue have never been re-biopsied because of a floater in with their tissue. Good discussion and long overdue. I look forward to the day when it is no longer an automatic response from all involved that it is "Histology's fault". Hope you are enjoying your new adventures in retirement. See you next year in Canada! > > > > From: mamawooo@hotmail.com > > To: histonet@lists.utsouthwestern.edu > > Date: Wed, 5 Oct 2011 14:11:25 -1000 > > Subject: [Histonet] (no subject) > > > > > > Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets. There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue. The answers were surprisingly pretty much the same. It is not an issue! Now I understand how one company can make this claim as their stainer uses fresh stain on each slide. The explanations from the other companies were insulting and just plain did not make sense to me. I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath." Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places. I have seen them from the doctor's office or procedure room to the stainer and every step in between . Sometimes if the "floater" is in the block it is very difficult to determine where it originated. We can however eliminate the water bath and stainer as the origin in these cases. One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle. I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes. So, no, not all floaters float. I would love to hear feedback from others on this. I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it. I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 6 Oct 2011 17:37:57 +0200 From: "Gudrun Lang" Subject: [Histonet] "formol" To: Message-ID: <4C8F235CA0384093830B90BB552641EA@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Formol is an old brandname, I think for a desinfection reagent. Not really custom any more. Bye (another) Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rene J Buesa Gesendet: Donnerstag, 06. Oktober 2011 15:13 An: histonet@lists.utsouthwestern.edu; Jenny Vega Betreff: [Histonet] Difference between Neutral Buffered Formalin 10% and NBformaldehyde 3.7 to 4% Jenny: Formaldehyde is?the GAS methanal?that dissolves in water at a maximum concentration between 37-40% vol./vol. To prevent its polymerization, small amounts of mathanol (an alcohol)are added. The 10% solution formaldehyde contains between 3.7 and 4.0% of formaldehyde and is called "formalin" in many countries and "formol" in Europe. So formalin is a 3.7 to 4.0% solution of formaldehyde. So a 10% formalin = 3.7-4.0% formaldehyde. NBF is prepared by added phosphate salts to prevent the formalin to acidify and is used to fix tissues. It is a pitty that your supervisor has so little knowledge about the area she is supposed to supervise, but I am not do not know why, but I am not.?surprised. To your question: SHE is wrong, really wrong! Ren? J. ? --- On Wed, 10/5/11, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 5, 2011, 7:03 PM Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it? we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to? putrefied if they are put in that solution. Am I wrong or is she wrong? thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 6 Oct 2011 15:43:15 +0000 From: Kelly Cross Subject: [Histonet] control for Hemoglobin? To: "Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu)" Message-ID: <869848DDBB7C5D4896A569A38B814E690551CAD1@CVMMB01.cvm.tamu.edu> Content-Type: text/plain; charset="us-ascii" Hi Histonetters, What control should I use for Okajima's Stain for hemoglobin? Thank you for your help! Kelly Cross B.S., HT (ASCP) Medical Laboratory Supervisor Texas A&M University VTPB Histology Lab VMA bldg. 214 College Station, TX 77843-4467 Office: 979-862-3658 Lab: 979-845-5149 ------------------------------ Message: 11 Date: Thu, 6 Oct 2011 05:44:31 -1000 From: Janice Mahoney Subject: RE: [Histonet] Tissue left in processor To: , histo net , Message-ID: Content-Type: text/plain; charset="iso-8859-1" I agree with Rene, as long as the temp is only a few degrees above the melting point of the paraffin.Jan,Omaha > Date: Thu, 6 Oct 2011 07:13:54 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; rcharles@pa.gov > Subject: Re: [Histonet] Tissue left in processor > CC: > > I do not think that a well fixed, well processed tissue left in molten paraffin for 2 hours after the processor finished will have any adverse outcome. > Ren? J. > > --- On Thu, 10/6/11, Charles, Roger wrote: > > > From: Charles, Roger > Subject: [Histonet] Tissue left in processor > To: "Histonet (histonet@lists.utsouthwestern.edu)" > Date: Thursday, October 6, 2011, 10:05 AM > > > Hi All, > Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes? And if so what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing was completed? > Thanks so much. > Roger > > Roger Charles| Microbiologist II > Pennsylvania Veterinary Laboratory > 2305 North Cameron Street | Harrisburg, PA 17110 > Phone: 717.787.8808 | Fax: 717.772.3895 > www.agriculture.state.pa.us > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Thu, 06 Oct 2011 11:49:08 -0400 From: "Tammy Pickles" Subject: [Histonet] Imidazole buffer To: "Histonet" Message-ID: <4E8D7994.B440.007F.1@inspection.gc.ca> Content-Type: text/plain; charset="us-ascii" Hi, I am wondering if anyone out there can tell me the function of Imidazole, when used in a buffer. We are currently using it to make up our in house DAB solution. The results are fantastic with an overall increase in sensitivity with all of our antibodies. The only information I can find concerning the function is to help in blocking nonspecific staining. Does anyone have more information to share? Thanks in advance, Tammy ------------------------------ Message: 13 Date: Thu, 6 Oct 2011 09:59:32 -0600 From: "gayle callis" Subject: [Histonet] RE: DAPI on thicker sections, problem with gradient To: Message-ID: <000601cc8440$f1af4270$d50dc750$@bresnan.net> Content-Type: text/plain; charset="iso-8859-1" Carmen, You wrote: Good morning: I am Carmen Garcia from the Faculty of Medicine of the University of Valencia, Spain. I usually use your vectashield mounting medium for fluorescence with DAPI (H-1200) and I never have any problem. But now I am working with 50um frozen sections and the problem that I have is that the DAPI staining does not arrive to the bottom of the section. Do you have any sugesstion of what can I do? (I already have the sections permeabilized with triton x-100) I have thougth to incubate with the mounting medium some hours at 4?C or put some mounting medium in the antibody diluent and incubate... any suggestion would be really welcome. Thank you so much, Kind regards, Carmen **************************************************************************** *********** The problem you are experiencing is what we refer to as a DAPI staining gradient. Uneven staining occurs with the DAPI is not reaching all the cells due to viscosity of the mounting media and thickness of the tissue section. You did not say whether your thick sections have been fixed before sectioning and staining for a tissue component, and then the DAPI - please explain. I suggest you do NOT use a mounting media with DAPI, but rather make a DAPI solution which you can then apply to the section and incubate mounting the coverslip with Vectashield that does not contain DAPI. Using a DAPI solution may shorten the time you now use, e.g. "some hours". You can buy the DAPI, ready to use from Biogenex or other vendors, but making it in house is simple. I would assume you could add Triton X to the DAPI solution to improve permeability and match what you have already done with that detergent. After DAPI staining, mount the coverslip using VectaShield without DAPI. DAPI recipes can be found on the internet, just Google DAPI. I found it on a Johns Hopkins webpage, but IHC world may have it too. There are other sources of ready to use DAPI if you look. We have used Prolong Gold antifade reagent with DAPI, and get a staining gradient on 5 um sections. This is very annoying, and probably caused by the actual technique of cover slipping itself. We are now going to use Biogenex ready to use DAPI before the Prolong Gold antifade reagent without DAPUI. Staining for a thin section takes 5 mintues at RT, so you should try several 50 um sections (non experimental) to determine your optimal staining time. It may take only 15 minutes although cold, 4C temperature may slow down the penetration of the stain through the thicker section. This way you can achieve even staining of your nuclei without long incubations in the more viscious mounting media containing DAPI. Good luck, and let us know if you have success................... Gayle M. Callis HTL/HT/MT(ASCP) ------------------------------ Message: 14 Date: Thu, 6 Oct 2011 16:06:06 +0000 From: Helen Fedor Subject: RE: [Histonet] RE: DAPI on thicker sections, problem with gradient To: 'gayle callis' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello, Maybe this method was meant to be used on Floating sections instead of mounted sections. That way the binding and penetration would go from both sides of the tissue. Helen L. Fedor http://tmalab.jhmi.edu/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Thursday, October 06, 2011 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: DAPI on thicker sections, problem with gradient Carmen, You wrote: Good morning: I am Carmen Garcia from the Faculty of Medicine of the University of Valencia, Spain. I usually use your vectashield mounting medium for fluorescence with DAPI (H-1200) and I never have any problem. But now I am working with 50um frozen sections and the problem that I have is that the DAPI staining does not arrive to the bottom of the section. Do you have any sugesstion of what can I do? (I already have the sections permeabilized with triton x-100) I have thougth to incubate with the mounting medium some hours at 4?C or put some mounting medium in the antibody diluent and incubate... any suggestion would be really welcome. Thank you so much, Kind regards, Carmen **************************************************************************** *********** The problem you are experiencing is what we refer to as a DAPI staining gradient. Uneven staining occurs with the DAPI is not reaching all the cells due to viscosity of the mounting media and thickness of the tissue section. You did not say whether your thick sections have been fixed before sectioning and staining for a tissue component, and then the DAPI - please explain. I suggest you do NOT use a mounting media with DAPI, but rather make a DAPI solution which you can then apply to the section and incubate mounting the coverslip with Vectashield that does not contain DAPI. Using a DAPI solution may shorten the time you now use, e.g. "some hours". You can buy the DAPI, ready to use from Biogenex or other vendors, but making it in house is simple. I would assume you could add Triton X to the DAPI solution to improve permeability and match what you have already done with that detergent. After DAPI staining, mount the coverslip using VectaShield without DAPI. DAPI recipes can be found on the internet, just Google DAPI. I found it on a Johns Hopkins webpage, but IHC world may have it too. There are other sources of ready to use DAPI if you look. We have used Prolong Gold antifade reagent with DAPI, and get a staining gradient on 5 um sections. This is very annoying, and probably caused by the actual technique of cover slipping itself. We are now going to use Biogenex ready to use DAPI before the Prolong Gold antifade reagent without DAPUI. Staining for a thin section takes 5 mintues at RT, so you should try several 50 um sections (non experimental) to determine your optimal staining time. It may take only 15 minutes although cold, 4C temperature may slow down the penetration of the stain through the thicker section. This way you can achieve even staining of your nuclei without long incubations in the more viscious mounting media containing DAPI. Good luck, and let us know if you have success................... Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 6 Oct 2011 16:09:28 +0000 From: Subject: [Histonet] RE: Cutting frozen sections from visceral fat To: Cc: histonet@lists.utsouthwestern.edu Message-ID: <14C3108E8B98EF43B3EDAE58336700340511A2@exchange.med.mun.ca> Content-Type: text/plain; charset="us-ascii" Hi Sarah, Thank you for the response. We are a research lab so whatever the researchers want to be done we do it with all kinds of tissues. In this case they are looking at the size of the fat cells in the animals. I will try to lower the temperature to -30 as was also suggested to me and if this does not work I will look into fixation. In Bancroft's fifth edition is mentioned that if you need fixation you need to use formol-calcium. I hope this will harden the fat and stabilize the membrane proteins enough so I can cut it. I will let you know how it worked. Thanks again, iliana -----Original Message----- From: Sarah Dysart [mailto:sdysart@mirnarx.com] Sent: October 6, 2011 12:14 PM To: Dimitrova, Iliana Subject: RE: Cutting frozen sections from visceral fat Cutting frozen fat is basically impossible. It will just goo up and rip out of the chuck of frozen OCT. I think there is a way where you can do a fixation process first in something, but I'm not sure of that. Why do they need frozen fat sections? Cutting fat after fixation is totally doable with the right fixation. I have done this when people have asked for frozen fat sections. I can usually talk them out of the frozen and just do the FFPE sections. Let me know I can give you more details. http://www.pathologyinnovations.com/new_page_2.htm This site has a section in it called wrestling the fat one, that has some ideas for you. Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of idimitro@mun.ca Sent: Thursday, October 06, 2011 6:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting frozen sections from visceral fat Hi everyone, I am having trouble cutting frozen sections from visceral fat. I have never cut only fat before. I am also cutting liver sections and they are perfect, but when I try to cut the fat it is not working. The temperature of the cryostat is ( -20) degree Celsius. I wonder if anyone has ever done this. I was thinking that if I lower the temperature or fix the fat with formol-calcium I may have success. Iliana Dimitrova, MSc, RT Histology Supervisor Room 2808 Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University St. John's, NL This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php ------------------------------ Message: 16 Date: Thu, 6 Oct 2011 12:30:02 -0400 From: "Dessoye, Michael J" Subject: [Histonet] CMV/HSV Protocols To: Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone have protocols for CMV, HSV I, and HSV II (from Cell Marque) that they would be willing to share for the Benchmark Ultra (i-View)? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ------------------------------ Message: 17 Date: Thu, 6 Oct 2011 16:39:38 +0000 From: joelle weaver Subject: RE: [Histonet] Floaters To: , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" This was an excellent article, I archived it, interesting that your own experiments confirmed this results. Joelle Weaver MAOM, BA, (HTL) ASCP > From: Timothy.Morken@ucsfmedctr.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 6 Oct 2011 08:19:25 -0700 > Subject: RE: [Histonet] Floaters > > The recent paper by Platt et all (Tissue Floaters and Contaminants in the Histology Laboratory, Arch Pathol Lab Med, Vol 133, June 2009) details floaters from waterbaths and staining process pretty well. > > Waterbaths do not appear to be a contributor. They literally found only ONE tissue fragment in waterbath water saved from an entire day of sectioning from 13 waterbaths. And it was in the water, not on a slide. They apparently did not identify ANY waterbath-derived floaters on any slide. They attribute this finding to the fact the tissue is still in paraffin so the tissue was either on the slide or stuck to the side of the waterbath, not floating in the water. > > To determine the extent of staining-derived floaters they measured the amount of tissue fragments found in each staining station. The vast majority were in the first xylene and alcohol baths. After the 95% alcohol there were only very rare tissue fragments in the rest of the baths. > > They found the staining process does contribute floaters (besides those that are clearly in the block), but apparently the do not derive from the fragments in bottom of the reagent containers. The floaters were from the other slides in the specific rack stained. The key finding was that BLANK slides put through a stainer at the end of the day (after staining over 1300 slides and accumulating the maximum amount of tissue fragments in the reagent baths) DID NOT PICK UP ANY FLOATERS. > > ALL the floaters that they determined had come from the staining process occurred ONLY when tissue slides were included in the stain rack (They alternated blank and tissue slides in the rack). So, the tissue seen in the stain dish does not appear to contribute to floaters. > > A stain instrument vendor repeated this experiment in our lab and came up with the same result. > > Our pathologists were mixed on the floater question. Some felt a system that eliminated floaters would be beneficial. Others thought floaters were easily detected and the vast majority could be ignored. It remains to be determined it is cost effective to replace the current system with one that would eliminate floaters. It does not appear to be a sole reason to replace the current system. > > Tim Morken > Supervisor, Histology, IPOX > UCSF Medical Center > San Francisco, CA, USA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, October 06, 2011 6:34 AM > To: histonet@lists.utsouthwestern.edu; Janice Mahoney > Subject: [Histonet] Floaters > > This is a very interesting subject indeed. To start I always take "with a grain of salt" whatever a vendor tells me because their opinions may (or may not, but just in case) biased to the products they sell. The same thing goes for any automobiles salesperson of any automobile make claiming the "wonders" of the make they sell compared with other makes. > Now, to the floater. I have also seen pieces of sections on the bottom of staining dishes. They are there because they fell-off the stained section. My question is: what are the possibilities of that piece of lost stained section that fell from its original slide, "re-float" and magically adhere to another slide? I really think that the possibilities range from almost none, to none. > Going to the water bath: yes, this is a major source of floaters because a piece of a section left in the water bath can be picked up in another slide along with a section from another case. That is why it is mandatory to swipe the surface of the water bath with a tissue after each case is cut. The source can be defined if we keep a record of the sequence in which the blocks are cut (as was standard procedure at my lab). > Whenever we ended with a floater in a section we went immediately to the block to determine if the floater came from the block or the water bath. > The other sources, either during description, cassetting or embedding are going to end in the block and it is very difficult to determine the origin. The only thing to do is to make sure that embedding wells where the forceps rest and heat, are thoroughly cleaned when the embedding ends. > But I think that the main thrust of the question deals with floaters coming from the staining dishes: as I wrote earlier I give to this source a very slim and almost impossible chance. > Ren? J. > > > > > --- On Wed, 10/5/11, Janice Mahoney wrote: > > > From: Janice Mahoney > Subject: [Histonet] (no subject) > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, October 5, 2011, 8:11 PM > > > > Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets. There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue. The answers were surprisingly pretty much the same. It is not an issue! Now I understand how one company can make this claim as their stainer uses fresh stain on each slide. The explanations from the other companies were insulting and just plain did not make sense to me. I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath." Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places. I have seen them from the doctor's office or procedure room to > the stainer and every step in between. Sometimes if the "floater" is in the block it is very difficult to determine where it originated. We can however eliminate the water bath and stainer as the origin in these cases. One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle. I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes. So, no, not all floaters float. I would love to hear feedback from others on this. I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it. I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Thu, 6 Oct 2011 16:55:09 +0000 From: joelle weaver Subject: RE: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% To: , , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I like this article's discussion of the history of usuage and terminology: citation Fox, C., Johnson, F., Whiting, J., and Roller, P. Formaldehyde Fixation, The Journal of Histochemistry and Cytochemistry, Vol. 33, No.8, pp. 845-853, 1985. Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Thu, 6 Oct 2011 08:56:55 -0600 > From: sbreeden@nmda.nmsu.edu > To: Gudrun.Erikstad@stolav.no; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% > CC: > > Now, wait just a minute! Don't tell me that after 40+ years of being a > histotech that I've been WRONG all this time??? FORMALDEHYDE is > FORMALDEHYDE is FORMALDEHYDE. FORMALIN is the diluted form of > FORMALDEHYDE, buffered and used at (usually) 10%. If I'm wrong, this > will be my last day at work... :) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Thu, 6 Oct 2011 10:55:11 -0600 From: "gayle callis" Subject: RE: [Histonet] Tissue left in processor To: "'Janice Mahoney'" , , "'histo net'" , Message-ID: <000001cc8448$b806d8f0$28148ad0$@bresnan.net> Content-Type: text/plain; charset="iso-8859-1" Some prefer to take the cassettes off the processor and let them harden until embedding can be done, rather than leave in molten, hot paraffin. This does not damage the tissue, and placing the cooled cassettes into the embedding center holding area allows the paraffin to re-melt in a shorter time before embedding. This has been discussed in the past on Histonet. Some tissues may dry out even more WITH difficult sectioning after prolonged heat exposure. One result will be a parched earth effect seen in sections. I see you are from a veterinary facility, and if you work with rodent tissues e.g. spleen and liver, you should not let the tissue sit in paraffin or you will have little hard rocks to section. If your sectioning suffers (dry, friable, shattered, hard) after allowing tissues to sit in hot paraffin as compared to the times when you embed asap after processing is finished, then over exposure to hot paraffin can contributing factor. I personally do not like to "cook" my tissue any longer than necessary and heat labile antigens will also be at risk. I schedule so I can be there to embed when the processing is completed. Our standard is to embed when processing is finished and schedule accordingly. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Thursday, October 06, 2011 9:45 AM To: rjbuesa@yahoo.com; histo net; rcharles@pa.gov Subject: RE: [Histonet] Tissue left in processor I agree with Rene, as long as the temp is only a few degrees above the melting point of the paraffin.Jan,Omaha > Date: Thu, 6 Oct 2011 07:13:54 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; rcharles@pa.gov > Subject: Re: [Histonet] Tissue left in processor > CC: > > I do not think that a well fixed, well processed tissue left in molten paraffin for 2 hours after the processor finished will have any adverse outcome. > Ren? J. > > --- On Thu, 10/6/11, Charles, Roger wrote: > > > From: Charles, Roger > Subject: [Histonet] Tissue left in processor > To: "Histonet (histonet@lists.utsouthwestern.edu)" > > Date: Thursday, October 6, 2011, 10:05 AM > > > Hi All, > Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes? And if so what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing was completed? > Thanks so much. > Roger > > Roger Charles| Microbiologist II > Pennsylvania Veterinary Laboratory > 2305 North Cameron Street | Harrisburg, PA 17110 > Phone: 717.787.8808 | Fax: 717.772.3895 > www.agriculture.state.pa.us ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 95, Issue 9 *************************************** From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Oct 6 12:49:01 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Oct 6 12:49:36 2011 Subject: [Histonet] RE: processor options? In-Reply-To: References: Message-ID: That is an awesome idea. You should get some money rights for that idea. . Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Featherstone, Annette [af46@buffalo.edu] Sent: Thursday, October 06, 2011 1:15 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] processor options? Maybe someone can come up with a tissue processor that will have an option that will drain the paraffin after a time limit and let tissue cassettes harden in a cooled retort. This would be invaluable to all conscientious histotechs who have tried to make it in to embed when the weather has shut down roads for extended periods of time. Or if you are a "lone" technician who has a family emergency and cannot get in to take the tissue off the machine. How about it, Leica, Sakura, Thermo? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, October 06, 2011 12:58 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 95, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Tissue left in processor (Rene J Buesa) 2. RE: Tissue left in processor (Sherwood, Margaret) 3. SV: [Histonet] Difference between Neutral Buffered Formalin 10% and NBformaldehyde 3.7 to 4% (Erikstad, Gudrun Hovstein) 4. Re: (no subject) (Angela Bitting) 5. RE: Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% (Breeden, Sara) 6. Ken Marissael is out of the office (Ken_Marissael@vwr.com) 7. RE: Floaters (Morken, Timothy) 8. RE: (no subject) (Janice Mahoney) 9. "formol" (Gudrun Lang) 10. control for Hemoglobin? (Kelly Cross) 11. RE: Tissue left in processor (Janice Mahoney) 12. Imidazole buffer (Tammy Pickles) 13. RE: DAPI on thicker sections, problem with gradient (gayle callis) 14. RE: RE: DAPI on thicker sections, problem with gradient (Helen Fedor) 15. RE: Cutting frozen sections from visceral fat (idimitro@mun.ca) 16. CMV/HSV Protocols (Dessoye, Michael J) 17. RE: Floaters (joelle weaver) 18. RE: Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% (joelle weaver) 19. RE: Tissue left in processor (gayle callis) ---------------------------------------------------------------------- Message: 1 Date: Thu, 6 Oct 2011 07:13:54 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Tissue left in processor To: "Histonet \(histonet@lists.utsouthwestern.edu\)" , RogerCharles Message-ID: <1317910434.70026.YahooMailClassic@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I do not think that a well fixed, well processed tissue left in molten paraffin for 2 hours after the processor finished will have any adverse outcome. Ren? J. --- On Thu, 10/6/11, Charles, Roger wrote: From: Charles, Roger Subject: [Histonet] Tissue left in processor To: "Histonet (histonet@lists.utsouthwestern.edu)" Date: Thursday, October 6, 2011, 10:05 AM Hi All, Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes?? And if so? what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing was completed? Thanks so much. Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 6 Oct 2011 10:17:43 -0400 From: "Sherwood, Margaret" Subject: RE: [Histonet] Tissue left in processor To: "Charles, Roger" , Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A8C@PHSXMB30.partners.org> Content-Type: text/plain; charset="us-ascii" I don't think you want to leave it in the processor for 2 hours. Even at the embedding center, if someone can't come to help orient the tissue, we then take the cassettes out of the paraffin station and leave on a paper towel. Have done that for sometimes 2-3 days. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Thursday, October 06, 2011 10:05 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Tissue left in processor Hi All, Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes? And if so what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing was completed? Thanks so much. Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 3 Date: Thu, 6 Oct 2011 16:22:55 +0200 From: "Erikstad, Gudrun Hovstein" Subject: SV: [Histonet] Difference between Neutral Buffered Formalin 10% and NBformaldehyde 3.7 to 4% To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I suppose Ren? meant to say: Formalin is a 37-40% solution of formaldehyde. 10% formalin is a 3,7-4,0% solution of formaldehyde. I've never heard the use of "Formol", but then again, I'm scandinavian, not som much european... ;-) Gudrun, Norway -----Opprinnelig melding----- Fra: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] P? vegne av Rene J Buesa Sendt: 6. oktober 2011 15:13 Til: histonet@lists.utsouthwestern.edu; Jenny Vega Emne: [Histonet] Difference between Neutral Buffered Formalin 10% and NBformaldehyde 3.7 to 4% Jenny: Formaldehyde is?the GAS methanal?that dissolves in water at a maximum concentration between 37-40% vol./vol. To prevent its polymerization, small amounts of mathanol (an alcohol)are added. The 10% solution formaldehyde contains between 3.7 and 4.0% of formaldehyde and is called "formalin" in many countries and "formol" in Europe. So formalin is a 3.7 to 4.0% solution of formaldehyde. So a 10% formalin = 3.7-4.0% formaldehyde. NBF is prepared by added phosphate salts to prevent the formalin to acidify and is used to fix tissues. It is a pitty that your supervisor has so little knowledge about the area she is supposed to supervise, but I am not do not know why, but I am not.?surprised. To your question: SHE is wrong, really wrong! Ren? J. ? --- On Wed, 10/5/11, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 5, 2011, 7:03 PM Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it? we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to? putrefied if they are put in that solution. Am I wrong or is she wrong? thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 06 Oct 2011 10:31:53 -0400 From: "Angela Bitting" Subject: Re: [Histonet] (no subject) To: "Janice Mahoney" , Message-ID: <4E8D8398.2B7F.00C9.1@geisinger.edu> Content-Type: text/plain; charset="us-ascii" I too spoke to one vendor who claimed that the floaters are not an issue and that their bottles drew below the level of the "floating" floaters. I didn't feel insulted, but did feel that this salesperson obviously had no actual lab experience or they never would have made such a ridiculous claim. I always put the opinions and experiences of other Histotechs far ahead of anything I hear from sales reps. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 >>> Janice Mahoney 10/5/2011 8:11 PM >>> Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets. There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue. The answers were surprisingly pretty much the same. It is not an issue! Now I understand how one company can make this claim as their stainer uses fresh stain on each slide. The explanations from the other companies were insulting and just plain did not make sense to me. I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath." Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places. I have seen them from the doctor's office or procedure room to the stainer and every step in be tween. Sometimes if the "floater" is in the block it is very difficult to determine where it originated. We can however eliminate the water bath and stainer as the origin in these cases. One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle. I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes. So, no, not all floaters float. I would love to hear feedback from others on this. I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it. I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 5 Date: Thu, 6 Oct 2011 08:56:55 -0600 From: "Breeden, Sara" Subject: RE: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% To: "Erikstad, Gudrun Hovstein" , Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFB21@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Now, wait just a minute! Don't tell me that after 40+ years of being a histotech that I've been WRONG all this time??? FORMALDEHYDE is FORMALDEHYDE is FORMALDEHYDE. FORMALIN is the diluted form of FORMALDEHYDE, buffered and used at (usually) 10%. If I'm wrong, this will be my last day at work... :) ------------------------------ Message: 6 Date: Thu, 6 Oct 2011 11:01:12 -0400 From: Ken_Marissael@vwr.com Subject: [Histonet] Ken Marissael is out of the office To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 10/06/2011 and will not return until 10/12/2011. I will be away on 10/06 and return 10/12. I will have limited e-mail and cell phone access, but will try to get back to you as quickly as possible. While I am away, please contact VWR Healthcare Customer Service at 877-881-1192. If you require immediate attenion, please contact Jackie Zerillo at 609-410-6152. She is the NYC rep. ------------------------------ Message: 7 Date: Thu, 6 Oct 2011 08:19:25 -0700 From: "Morken, Timothy" Subject: RE: [Histonet] Floaters To: "histonet@lists.utsouthwestern.edu" Message-ID: <8D7C2D242DBD45498006B21122072BF89448A27E@MCINFRWEM003.ucsfmedicalcenter.org> Content-Type: text/plain; charset=iso-8859-1 The recent paper by Platt et all (Tissue Floaters and Contaminants in the Histology Laboratory, Arch Pathol Lab Med, Vol 133, June 2009) details floaters from waterbaths and staining process pretty well. Waterbaths do not appear to be a contributor. They literally found only ONE tissue fragment in waterbath water saved from an entire day of sectioning from 13 waterbaths. And it was in the water, not on a slide. They apparently did not identify ANY waterbath-derived floaters on any slide. They attribute this finding to the fact the tissue is still in paraffin so the tissue was either on the slide or stuck to the side of the waterbath, not floating in the water. To determine the extent of staining-derived floaters they measured the amount of tissue fragments found in each staining station. The vast majority were in the first xylene and alcohol baths. After the 95% alcohol there were only very rare tissue fragments in the rest of the baths. They found the staining process does contribute floaters (besides those that are clearly in the block), but apparently the do not derive from the fragments in bottom of the reagent containers. The floaters were from the other slides in the specific rack stained. The key finding was that BLANK slides put through a stainer at the end of the day (after staining over 1300 slides and accumulating the maximum amount of tissue fragments in the reagent baths) DID NOT PICK UP ANY FLOATERS. ALL the floaters that they determined had come from the staining process occurred ONLY when tissue slides were included in the stain rack (They alternated blank and tissue slides in the rack). So, the tissue seen in the stain dish does not appear to contribute to floaters. A stain instrument vendor repeated this experiment in our lab and came up with the same result. Our pathologists were mixed on the floater question. Some felt a system that eliminated floaters would be beneficial. Others thought floaters were easily detected and the vast majority could be ignored. It remains to be determined it is cost effective to replace the current system with one that would eliminate floaters. It does not appear to be a sole reason to replace the current system. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 06, 2011 6:34 AM To: histonet@lists.utsouthwestern.edu; Janice Mahoney Subject: [Histonet] Floaters This is a very interesting subject indeed. To start I always take "with a grain of salt" whatever a vendor tells me because their opinions may (or may not, but just in case) biased to the products they sell. The same thing goes for any automobiles salesperson of any automobile make claiming the "wonders" of the make they sell compared with other makes. Now, to the floater. I have also seen pieces of sections on the bottom of staining dishes. They are there because they fell-off the stained section. My question is: what are the possibilities of that piece of lost stained section that fell from its original slide, "re-float" and magically adhere to another slide? I really think that the possibilities range from almost none, to none. Going to the water bath: yes, this is a major source of floaters because a piece of a section left in the water bath can be picked up in another slide along with a section from another case. That is why it is mandatory to swipe the surface of the water bath with? a tissue after each case is cut. The source can be defined if we keep a record of the sequence in which the blocks are cut (as was standard procedure at my lab). Whenever we ended with a floater in a section we went immediately to the block to determine if the floater came from the block or the water bath. The other sources, either during description, cassetting or embedding are going to end in the block and it is very difficult to determine the origin. The only thing to do is to make sure that embedding wells where the forceps rest and heat, are thoroughly cleaned when the embedding ends. But I think that the main thrust of the question deals with floaters coming from the staining dishes: as I wrote earlier I give to this source?a very slim and almost impossible chance. Ren? J. ? ? ? --- On Wed, 10/5/11, Janice Mahoney wrote: From: Janice Mahoney Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 5, 2011, 8:11 PM Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets.? There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue.? The answers were surprisingly pretty much the same.???It is not an issue!? Now I understand how one company can make this claim as their stainer uses fresh stain on each slide.? The explanations from the other companies were insulting and just plain did not make sense to me.? I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath."? Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places.? I have seen them from the doctor's office or procedure room to the stainer and every step in between.? Sometimes if the "floater" is in the block it is very difficult to determine where it originated.? We can however eliminate the water bath and stainer as the origin in these cases.? One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle.? I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes.? So, no, not all floaters float.? I would love to hear feedback from others on this.? I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it.? I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 6 Oct 2011 05:23:53 -1000 From: Janice Mahoney Subject: RE: [Histonet] (no subject) To: Cindy Robinson , histo net , Christie Gowan Message-ID: Content-Type: text/plain; charset="iso-8859-1" Very good question Cindi.Jan > Date: Thu, 6 Oct 2011 09:26:18 -0400 > From: robinsoc@mercyhealth.com > To: mamawooo@hotmail.com; histonet@lists.utsouthwestern.edu; christiegowan@msn.com > Subject: RE: [Histonet] (no subject) > > It is a good thing this vendor does not work in a histo lab because with that comment/attitude he/she would not last long. I have heard the water bath theory but upon investigation this has never been the source because every histotech I work with cleans it each and every time. We have traced floaters back to grossing stations, processors...especially placenta getting 'snagged' on a piece of bone...embedding forceps (we now use ones with no grooves), forcep warmers, stains and coverslippers. We have significantly reduced the number we see since we make the effort to track each one found as an ongoing QA project, but we still see the occasional floater. We did find that we have to put tissue types which fragment easily into mesh cassettes or bags but this can cause issues with fluid exchange and carryover during processing. It is a balancing act. I have always wondering about the newer processors with orientation cassettes which are embed and then cut without opening. Do th ey see less floaters with this type of 'closed' system? > > > > Cindi Robinson HT(ASCP) > Mercy Medical Center > Dunes Medical Laboratories > 350 W Anchor Dr > Dakota Dunes SD 57049 > phone-712-279-2768 > robinsoc@mercyhealth.com > > > >>> CHRISTIE GOWAN 10/6/2011 7:44 AM >>> > > I agree with you Jan. In my 30 + years as a histotech (yes, I am old too) I have seen floaters come from a variety of places but I am hard pressed to remember any floater coming from the water bath. Today we are blessed with DNA fingerprinting to determine if the floater is or is not from the patient but that still does not address the real issue of where did it come from and how do we stop it. The vendors stating that it is not an issue have never been re-biopsied because of a floater in with their tissue. Good discussion and long overdue. I look forward to the day when it is no longer an automatic response from all involved that it is "Histology's fault". Hope you are enjoying your new adventures in retirement. See you next year in Canada! > > > > From: mamawooo@hotmail.com > > To: histonet@lists.utsouthwestern.edu > > Date: Wed, 5 Oct 2011 14:11:25 -1000 > > Subject: [Histonet] (no subject) > > > > > > Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets. There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue. The answers were surprisingly pretty much the same. It is not an issue! Now I understand how one company can make this claim as their stainer uses fresh stain on each slide. The explanations from the other companies were insulting and just plain did not make sense to me. I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath." Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places. I have seen them from the doctor's office or procedure room to the stainer and every step in between . Sometimes if the "floater" is in the block it is very difficult to determine where it originated. We can however eliminate the water bath and stainer as the origin in these cases. One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle. I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes. So, no, not all floaters float. I would love to hear feedback from others on this. I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it. I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 6 Oct 2011 17:37:57 +0200 From: "Gudrun Lang" Subject: [Histonet] "formol" To: Message-ID: <4C8F235CA0384093830B90BB552641EA@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Formol is an old brandname, I think for a desinfection reagent. Not really custom any more. Bye (another) Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rene J Buesa Gesendet: Donnerstag, 06. Oktober 2011 15:13 An: histonet@lists.utsouthwestern.edu; Jenny Vega Betreff: [Histonet] Difference between Neutral Buffered Formalin 10% and NBformaldehyde 3.7 to 4% Jenny: Formaldehyde is?the GAS methanal?that dissolves in water at a maximum concentration between 37-40% vol./vol. To prevent its polymerization, small amounts of mathanol (an alcohol)are added. The 10% solution formaldehyde contains between 3.7 and 4.0% of formaldehyde and is called "formalin" in many countries and "formol" in Europe. So formalin is a 3.7 to 4.0% solution of formaldehyde. So a 10% formalin = 3.7-4.0% formaldehyde. NBF is prepared by added phosphate salts to prevent the formalin to acidify and is used to fix tissues. It is a pitty that your supervisor has so little knowledge about the area she is supposed to supervise, but I am not do not know why, but I am not.?surprised. To your question: SHE is wrong, really wrong! Ren? J. ? --- On Wed, 10/5/11, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 5, 2011, 7:03 PM Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it? we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to? putrefied if they are put in that solution. Am I wrong or is she wrong? thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 6 Oct 2011 15:43:15 +0000 From: Kelly Cross Subject: [Histonet] control for Hemoglobin? To: "Histonet Listserv (E-mail) (histonet@lists.utsouthwestern.edu)" Message-ID: <869848DDBB7C5D4896A569A38B814E690551CAD1@CVMMB01.cvm.tamu.edu> Content-Type: text/plain; charset="us-ascii" Hi Histonetters, What control should I use for Okajima's Stain for hemoglobin? Thank you for your help! Kelly Cross B.S., HT (ASCP) Medical Laboratory Supervisor Texas A&M University VTPB Histology Lab VMA bldg. 214 College Station, TX 77843-4467 Office: 979-862-3658 Lab: 979-845-5149 ------------------------------ Message: 11 Date: Thu, 6 Oct 2011 05:44:31 -1000 From: Janice Mahoney Subject: RE: [Histonet] Tissue left in processor To: , histo net , Message-ID: Content-Type: text/plain; charset="iso-8859-1" I agree with Rene, as long as the temp is only a few degrees above the melting point of the paraffin.Jan,Omaha > Date: Thu, 6 Oct 2011 07:13:54 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; rcharles@pa.gov > Subject: Re: [Histonet] Tissue left in processor > CC: > > I do not think that a well fixed, well processed tissue left in molten paraffin for 2 hours after the processor finished will have any adverse outcome. > Ren? J. > > --- On Thu, 10/6/11, Charles, Roger wrote: > > > From: Charles, Roger > Subject: [Histonet] Tissue left in processor > To: "Histonet (histonet@lists.utsouthwestern.edu)" > Date: Thursday, October 6, 2011, 10:05 AM > > > Hi All, > Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes? And if so what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing was completed? > Thanks so much. > Roger > > Roger Charles| Microbiologist II > Pennsylvania Veterinary Laboratory > 2305 North Cameron Street | Harrisburg, PA 17110 > Phone: 717.787.8808 | Fax: 717.772.3895 > www.agriculture.state.pa.us > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Thu, 06 Oct 2011 11:49:08 -0400 From: "Tammy Pickles" Subject: [Histonet] Imidazole buffer To: "Histonet" Message-ID: <4E8D7994.B440.007F.1@inspection.gc.ca> Content-Type: text/plain; charset="us-ascii" Hi, I am wondering if anyone out there can tell me the function of Imidazole, when used in a buffer. We are currently using it to make up our in house DAB solution. The results are fantastic with an overall increase in sensitivity with all of our antibodies. The only information I can find concerning the function is to help in blocking nonspecific staining. Does anyone have more information to share? Thanks in advance, Tammy ------------------------------ Message: 13 Date: Thu, 6 Oct 2011 09:59:32 -0600 From: "gayle callis" Subject: [Histonet] RE: DAPI on thicker sections, problem with gradient To: Message-ID: <000601cc8440$f1af4270$d50dc750$@bresnan.net> Content-Type: text/plain; charset="iso-8859-1" Carmen, You wrote: Good morning: I am Carmen Garcia from the Faculty of Medicine of the University of Valencia, Spain. I usually use your vectashield mounting medium for fluorescence with DAPI (H-1200) and I never have any problem. But now I am working with 50um frozen sections and the problem that I have is that the DAPI staining does not arrive to the bottom of the section. Do you have any sugesstion of what can I do? (I already have the sections permeabilized with triton x-100) I have thougth to incubate with the mounting medium some hours at 4?C or put some mounting medium in the antibody diluent and incubate... any suggestion would be really welcome. Thank you so much, Kind regards, Carmen **************************************************************************** *********** The problem you are experiencing is what we refer to as a DAPI staining gradient. Uneven staining occurs with the DAPI is not reaching all the cells due to viscosity of the mounting media and thickness of the tissue section. You did not say whether your thick sections have been fixed before sectioning and staining for a tissue component, and then the DAPI - please explain. I suggest you do NOT use a mounting media with DAPI, but rather make a DAPI solution which you can then apply to the section and incubate mounting the coverslip with Vectashield that does not contain DAPI. Using a DAPI solution may shorten the time you now use, e.g. "some hours". You can buy the DAPI, ready to use from Biogenex or other vendors, but making it in house is simple. I would assume you could add Triton X to the DAPI solution to improve permeability and match what you have already done with that detergent. After DAPI staining, mount the coverslip using VectaShield without DAPI. DAPI recipes can be found on the internet, just Google DAPI. I found it on a Johns Hopkins webpage, but IHC world may have it too. There are other sources of ready to use DAPI if you look. We have used Prolong Gold antifade reagent with DAPI, and get a staining gradient on 5 um sections. This is very annoying, and probably caused by the actual technique of cover slipping itself. We are now going to use Biogenex ready to use DAPI before the Prolong Gold antifade reagent without DAPUI. Staining for a thin section takes 5 mintues at RT, so you should try several 50 um sections (non experimental) to determine your optimal staining time. It may take only 15 minutes although cold, 4C temperature may slow down the penetration of the stain through the thicker section. This way you can achieve even staining of your nuclei without long incubations in the more viscious mounting media containing DAPI. Good luck, and let us know if you have success................... Gayle M. Callis HTL/HT/MT(ASCP) ------------------------------ Message: 14 Date: Thu, 6 Oct 2011 16:06:06 +0000 From: Helen Fedor Subject: RE: [Histonet] RE: DAPI on thicker sections, problem with gradient To: 'gayle callis' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello, Maybe this method was meant to be used on Floating sections instead of mounted sections. That way the binding and penetration would go from both sides of the tissue. Helen L. Fedor http://tmalab.jhmi.edu/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Thursday, October 06, 2011 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: DAPI on thicker sections, problem with gradient Carmen, You wrote: Good morning: I am Carmen Garcia from the Faculty of Medicine of the University of Valencia, Spain. I usually use your vectashield mounting medium for fluorescence with DAPI (H-1200) and I never have any problem. But now I am working with 50um frozen sections and the problem that I have is that the DAPI staining does not arrive to the bottom of the section. Do you have any sugesstion of what can I do? (I already have the sections permeabilized with triton x-100) I have thougth to incubate with the mounting medium some hours at 4?C or put some mounting medium in the antibody diluent and incubate... any suggestion would be really welcome. Thank you so much, Kind regards, Carmen **************************************************************************** *********** The problem you are experiencing is what we refer to as a DAPI staining gradient. Uneven staining occurs with the DAPI is not reaching all the cells due to viscosity of the mounting media and thickness of the tissue section. You did not say whether your thick sections have been fixed before sectioning and staining for a tissue component, and then the DAPI - please explain. I suggest you do NOT use a mounting media with DAPI, but rather make a DAPI solution which you can then apply to the section and incubate mounting the coverslip with Vectashield that does not contain DAPI. Using a DAPI solution may shorten the time you now use, e.g. "some hours". You can buy the DAPI, ready to use from Biogenex or other vendors, but making it in house is simple. I would assume you could add Triton X to the DAPI solution to improve permeability and match what you have already done with that detergent. After DAPI staining, mount the coverslip using VectaShield without DAPI. DAPI recipes can be found on the internet, just Google DAPI. I found it on a Johns Hopkins webpage, but IHC world may have it too. There are other sources of ready to use DAPI if you look. We have used Prolong Gold antifade reagent with DAPI, and get a staining gradient on 5 um sections. This is very annoying, and probably caused by the actual technique of cover slipping itself. We are now going to use Biogenex ready to use DAPI before the Prolong Gold antifade reagent without DAPUI. Staining for a thin section takes 5 mintues at RT, so you should try several 50 um sections (non experimental) to determine your optimal staining time. It may take only 15 minutes although cold, 4C temperature may slow down the penetration of the stain through the thicker section. This way you can achieve even staining of your nuclei without long incubations in the more viscious mounting media containing DAPI. Good luck, and let us know if you have success................... Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 6 Oct 2011 16:09:28 +0000 From: Subject: [Histonet] RE: Cutting frozen sections from visceral fat To: Cc: histonet@lists.utsouthwestern.edu Message-ID: <14C3108E8B98EF43B3EDAE58336700340511A2@exchange.med.mun.ca> Content-Type: text/plain; charset="us-ascii" Hi Sarah, Thank you for the response. We are a research lab so whatever the researchers want to be done we do it with all kinds of tissues. In this case they are looking at the size of the fat cells in the animals. I will try to lower the temperature to -30 as was also suggested to me and if this does not work I will look into fixation. In Bancroft's fifth edition is mentioned that if you need fixation you need to use formol-calcium. I hope this will harden the fat and stabilize the membrane proteins enough so I can cut it. I will let you know how it worked. Thanks again, iliana -----Original Message----- From: Sarah Dysart [mailto:sdysart@mirnarx.com] Sent: October 6, 2011 12:14 PM To: Dimitrova, Iliana Subject: RE: Cutting frozen sections from visceral fat Cutting frozen fat is basically impossible. It will just goo up and rip out of the chuck of frozen OCT. I think there is a way where you can do a fixation process first in something, but I'm not sure of that. Why do they need frozen fat sections? Cutting fat after fixation is totally doable with the right fixation. I have done this when people have asked for frozen fat sections. I can usually talk them out of the frozen and just do the FFPE sections. Let me know I can give you more details. http://www.pathologyinnovations.com/new_page_2.htm This site has a section in it called wrestling the fat one, that has some ideas for you. Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of idimitro@mun.ca Sent: Thursday, October 06, 2011 6:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting frozen sections from visceral fat Hi everyone, I am having trouble cutting frozen sections from visceral fat. I have never cut only fat before. I am also cutting liver sections and they are perfect, but when I try to cut the fat it is not working. The temperature of the cryostat is ( -20) degree Celsius. I wonder if anyone has ever done this. I was thinking that if I lower the temperature or fix the fat with formol-calcium I may have success. Iliana Dimitrova, MSc, RT Histology Supervisor Room 2808 Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University St. John's, NL This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php ------------------------------ Message: 16 Date: Thu, 6 Oct 2011 12:30:02 -0400 From: "Dessoye, Michael J" Subject: [Histonet] CMV/HSV Protocols To: Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone have protocols for CMV, HSV I, and HSV II (from Cell Marque) that they would be willing to share for the Benchmark Ultra (i-View)? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ------------------------------ Message: 17 Date: Thu, 6 Oct 2011 16:39:38 +0000 From: joelle weaver Subject: RE: [Histonet] Floaters To: , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" This was an excellent article, I archived it, interesting that your own experiments confirmed this results. Joelle Weaver MAOM, BA, (HTL) ASCP > From: Timothy.Morken@ucsfmedctr.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 6 Oct 2011 08:19:25 -0700 > Subject: RE: [Histonet] Floaters > > The recent paper by Platt et all (Tissue Floaters and Contaminants in the Histology Laboratory, Arch Pathol Lab Med, Vol 133, June 2009) details floaters from waterbaths and staining process pretty well. > > Waterbaths do not appear to be a contributor. They literally found only ONE tissue fragment in waterbath water saved from an entire day of sectioning from 13 waterbaths. And it was in the water, not on a slide. They apparently did not identify ANY waterbath-derived floaters on any slide. They attribute this finding to the fact the tissue is still in paraffin so the tissue was either on the slide or stuck to the side of the waterbath, not floating in the water. > > To determine the extent of staining-derived floaters they measured the amount of tissue fragments found in each staining station. The vast majority were in the first xylene and alcohol baths. After the 95% alcohol there were only very rare tissue fragments in the rest of the baths. > > They found the staining process does contribute floaters (besides those that are clearly in the block), but apparently the do not derive from the fragments in bottom of the reagent containers. The floaters were from the other slides in the specific rack stained. The key finding was that BLANK slides put through a stainer at the end of the day (after staining over 1300 slides and accumulating the maximum amount of tissue fragments in the reagent baths) DID NOT PICK UP ANY FLOATERS. > > ALL the floaters that they determined had come from the staining process occurred ONLY when tissue slides were included in the stain rack (They alternated blank and tissue slides in the rack). So, the tissue seen in the stain dish does not appear to contribute to floaters. > > A stain instrument vendor repeated this experiment in our lab and came up with the same result. > > Our pathologists were mixed on the floater question. Some felt a system that eliminated floaters would be beneficial. Others thought floaters were easily detected and the vast majority could be ignored. It remains to be determined it is cost effective to replace the current system with one that would eliminate floaters. It does not appear to be a sole reason to replace the current system. > > Tim Morken > Supervisor, Histology, IPOX > UCSF Medical Center > San Francisco, CA, USA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, October 06, 2011 6:34 AM > To: histonet@lists.utsouthwestern.edu; Janice Mahoney > Subject: [Histonet] Floaters > > This is a very interesting subject indeed. To start I always take "with a grain of salt" whatever a vendor tells me because their opinions may (or may not, but just in case) biased to the products they sell. The same thing goes for any automobiles salesperson of any automobile make claiming the "wonders" of the make they sell compared with other makes. > Now, to the floater. I have also seen pieces of sections on the bottom of staining dishes. They are there because they fell-off the stained section. My question is: what are the possibilities of that piece of lost stained section that fell from its original slide, "re-float" and magically adhere to another slide? I really think that the possibilities range from almost none, to none. > Going to the water bath: yes, this is a major source of floaters because a piece of a section left in the water bath can be picked up in another slide along with a section from another case. That is why it is mandatory to swipe the surface of the water bath with a tissue after each case is cut. The source can be defined if we keep a record of the sequence in which the blocks are cut (as was standard procedure at my lab). > Whenever we ended with a floater in a section we went immediately to the block to determine if the floater came from the block or the water bath. > The other sources, either during description, cassetting or embedding are going to end in the block and it is very difficult to determine the origin. The only thing to do is to make sure that embedding wells where the forceps rest and heat, are thoroughly cleaned when the embedding ends. > But I think that the main thrust of the question deals with floaters coming from the staining dishes: as I wrote earlier I give to this source a very slim and almost impossible chance. > Ren? J. > > > > > --- On Wed, 10/5/11, Janice Mahoney wrote: > > > From: Janice Mahoney > Subject: [Histonet] (no subject) > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, October 5, 2011, 8:11 PM > > > > Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding "floaters" and the amount determined to come from traditional staining buckets. There was also a poster presented at the NSH this year on the subject.When I approached several vendors of H&E stainers about this issue. The answers were surprisingly pretty much the same. It is not an issue! Now I understand how one company can make this claim as their stainer uses fresh stain on each slide. The explanations from the other companies were insulting and just plain did not make sense to me. I was told by a Histo tech vendor that "All Histo techs know that floaters come from the water bath." Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places. I have seen them from the doctor's office or procedure room to > the stainer and every step in between. Sometimes if the "floater" is in the block it is very difficult to determine where it originated. We can however eliminate the water bath and stainer as the origin in these cases. One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle. I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes. So, no, not all floaters float. I would love to hear feedback from others on this. I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it. I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Thu, 6 Oct 2011 16:55:09 +0000 From: joelle weaver Subject: RE: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% To: , , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I like this article's discussion of the history of usuage and terminology: citation Fox, C., Johnson, F., Whiting, J., and Roller, P. Formaldehyde Fixation, The Journal of Histochemistry and Cytochemistry, Vol. 33, No.8, pp. 845-853, 1985. Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Thu, 6 Oct 2011 08:56:55 -0600 > From: sbreeden@nmda.nmsu.edu > To: Gudrun.Erikstad@stolav.no; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% > CC: > > Now, wait just a minute! Don't tell me that after 40+ years of being a > histotech that I've been WRONG all this time??? FORMALDEHYDE is > FORMALDEHYDE is FORMALDEHYDE. FORMALIN is the diluted form of > FORMALDEHYDE, buffered and used at (usually) 10%. If I'm wrong, this > will be my last day at work... :) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Thu, 6 Oct 2011 10:55:11 -0600 From: "gayle callis" Subject: RE: [Histonet] Tissue left in processor To: "'Janice Mahoney'" , , "'histo net'" , Message-ID: <000001cc8448$b806d8f0$28148ad0$@bresnan.net> Content-Type: text/plain; charset="iso-8859-1" Some prefer to take the cassettes off the processor and let them harden until embedding can be done, rather than leave in molten, hot paraffin. This does not damage the tissue, and placing the cooled cassettes into the embedding center holding area allows the paraffin to re-melt in a shorter time before embedding. This has been discussed in the past on Histonet. Some tissues may dry out even more WITH difficult sectioning after prolonged heat exposure. One result will be a parched earth effect seen in sections. I see you are from a veterinary facility, and if you work with rodent tissues e.g. spleen and liver, you should not let the tissue sit in paraffin or you will have little hard rocks to section. If your sectioning suffers (dry, friable, shattered, hard) after allowing tissues to sit in hot paraffin as compared to the times when you embed asap after processing is finished, then over exposure to hot paraffin can contributing factor. I personally do not like to "cook" my tissue any longer than necessary and heat labile antigens will also be at risk. I schedule so I can be there to embed when the processing is completed. Our standard is to embed when processing is finished and schedule accordingly. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Thursday, October 06, 2011 9:45 AM To: rjbuesa@yahoo.com; histo net; rcharles@pa.gov Subject: RE: [Histonet] Tissue left in processor I agree with Rene, as long as the temp is only a few degrees above the melting point of the paraffin.Jan,Omaha > Date: Thu, 6 Oct 2011 07:13:54 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; rcharles@pa.gov > Subject: Re: [Histonet] Tissue left in processor > CC: > > I do not think that a well fixed, well processed tissue left in molten paraffin for 2 hours after the processor finished will have any adverse outcome. > Ren? J. > > --- On Thu, 10/6/11, Charles, Roger wrote: > > > From: Charles, Roger > Subject: [Histonet] Tissue left in processor > To: "Histonet (histonet@lists.utsouthwestern.edu)" > > Date: Thursday, October 6, 2011, 10:05 AM > > > Hi All, > Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes? And if so what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing was completed? > Thanks so much. > Roger > > Roger Charles| Microbiologist II > Pennsylvania Veterinary Laboratory > 2305 North Cameron Street | Harrisburg, PA 17110 > Phone: 717.787.8808 | Fax: 717.772.3895 > www.agriculture.state.pa.us ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 95, Issue 9 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Thu Oct 6 13:16:57 2011 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Thu Oct 6 13:17:30 2011 Subject: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% In-Reply-To: References: <1317906775.25229.YahooMailClassic@web65703.mail.ac4.yahoo.com>, , <4D14F0FC9316DD41972D5F03C070908B051DFB21@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <493CAA64F203E14E8823737B9EE0E25F091E9126CB@EXCHMB01.isis.local> Formaldehyde is the concentrated form and formalin the diluted form of formaldehyde. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, October 06, 2011 9:55 AM To: sbreeden@nmda.nmsu.edu; gudrun.erikstad@stolav.no; Histonet Subject: RE: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% I like this article's discussion of the history of usuage and terminology: citation Fox, C., Johnson, F., Whiting, J., and Roller, P. Formaldehyde Fixation, The Journal of Histochemistry and Cytochemistry, Vol. 33, No.8, pp. 845-853, 1985. Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Thu, 6 Oct 2011 08:56:55 -0600 > From: sbreeden@nmda.nmsu.edu > To: Gudrun.Erikstad@stolav.no; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4% > CC: > > Now, wait just a minute! Don't tell me that after 40+ years of being a > histotech that I've been WRONG all this time??? FORMALDEHYDE is > FORMALDEHYDE is FORMALDEHYDE. FORMALIN is the diluted form of > FORMALDEHYDE, buffered and used at (usually) 10%. If I'm wrong, this > will be my last day at work... :) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From celebrej <@t> HHSC.CA Thu Oct 6 13:17:05 2011 From: celebrej <@t> HHSC.CA (Celebre Julia) Date: Thu Oct 6 13:18:06 2011 Subject: [Histonet] ATP 10's for muscle biopsies Message-ID: <722701352B76E841BFB9EFAF72553CD20107A9@IPEXMAILM01.hhsc.ca> I'm having issues with the ATP 10's we run on our muscle biopsies and am hoping those who are familiar with this stain are willing to share their methods?! Any help or advise is greatly appreciated. Thanks Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179 From MSHERWOOD <@t> PARTNERS.ORG Thu Oct 6 13:23:44 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Thu Oct 6 13:23:49 2011 Subject: [Histonet] Tissue left in processor In-Reply-To: <000001cc8448$b806d8f0$28148ad0$@bresnan.net> References: <3809C163DC1DA54AA534B3C7794D07B68EC20DC73B@ENHBGMBX01.PA.LCL>, <1317910434.70026.YahooMailClassic@web65710.mail.ac4.yahoo.com> <000001cc8448$b806d8f0$28148ad0$@bresnan.net> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A90@PHSXMB30.partners.org> That is precisely why we take the cassettes out of the molten paraffin (embedding center) if they cannot be embedded right away. I felt they were being "cooked" as well. We have had not problems sectioning cassettes that have hardened and re-melted. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Thursday, October 06, 2011 12:55 PM To: 'Janice Mahoney'; rjbuesa@yahoo.com; 'histo net'; rcharles@pa.gov Subject: RE: [Histonet] Tissue left in processor Some prefer to take the cassettes off the processor and let them harden until embedding can be done, rather than leave in molten, hot paraffin. This does not damage the tissue, and placing the cooled cassettes into the embedding center holding area allows the paraffin to re-melt in a shorter time before embedding. This has been discussed in the past on Histonet. Some tissues may dry out even more WITH difficult sectioning after prolonged heat exposure. One result will be a parched earth effect seen in sections. I see you are from a veterinary facility, and if you work with rodent tissues e.g. spleen and liver, you should not let the tissue sit in paraffin or you will have little hard rocks to section. If your sectioning suffers (dry, friable, shattered, hard) after allowing tissues to sit in hot paraffin as compared to the times when you embed asap after processing is finished, then over exposure to hot paraffin can contributing factor. I personally do not like to "cook" my tissue any longer than necessary and heat labile antigens will also be at risk. I schedule so I can be there to embed when the processing is completed. Our standard is to embed when processing is finished and schedule accordingly. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Thursday, October 06, 2011 9:45 AM To: rjbuesa@yahoo.com; histo net; rcharles@pa.gov Subject: RE: [Histonet] Tissue left in processor I agree with Rene, as long as the temp is only a few degrees above the melting point of the paraffin.Jan,Omaha > Date: Thu, 6 Oct 2011 07:13:54 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; rcharles@pa.gov > Subject: Re: [Histonet] Tissue left in processor > CC: > > I do not think that a well fixed, well processed tissue left in molten paraffin for 2 hours after the processor finished will have any adverse outcome. > Ren? J. > > --- On Thu, 10/6/11, Charles, Roger wrote: > > > From: Charles, Roger > Subject: [Histonet] Tissue left in processor > To: "Histonet (histonet@lists.utsouthwestern.edu)" > > Date: Thursday, October 6, 2011, 10:05 AM > > > Hi All, > Is there any standard on how long tissue cassettes can remain in the processor after processing before the tissue is subjected to unwanted outcomes? And if so what type of artifacts can one expect from tissue that was in the processor in molten paraffin for 2 hours after the processing was completed? > Thanks so much. > Roger > > Roger Charles| Microbiologist II > Pennsylvania Veterinary Laboratory > 2305 North Cameron Street | Harrisburg, PA 17110 > Phone: 717.787.8808 | Fax: 717.772.3895 > www.agriculture.state.pa.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From cindy38017 <@t> yahoo.com Thu Oct 6 13:41:36 2011 From: cindy38017 <@t> yahoo.com (cindy dewar) Date: Thu Oct 6 13:41:40 2011 Subject: [Histonet] Fontanta Masson on frozen sections Message-ID: <1317926496.44606.YahooMailClassic@web37106.mail.mud.yahoo.com> Has anyone performed a Fontana Masson of frozen section skin specimens. If so, would you mind sharing your protocol, especially the fixation step? Thanks, Cindy From nicole <@t> dlcjax.com Thu Oct 6 13:56:36 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Thu Oct 6 14:04:21 2011 Subject: [Histonet] Florida Society of Dermatologic Surgeons Message-ID: <3441.208.62.167.196.1317927396.squirrel@webmail.realpages.com> The Florida Society for Dermatologic Surgeons will be holding there 30th annual meeting at the Peabody Hotel in Orlando, Fl Dec 2-4, 2011. They are looking for venders or members who are intersted in setting up a booth. The physicians interests are in: Pharmaceutical. Equipment. Pathology labs. Mohs consultation.Cosmetics. Please contact me and I can fax you the required documentation. Nicole Tatum, HT ASCP From andrea.conard <@t> gmail.com Thu Oct 6 14:23:47 2011 From: andrea.conard <@t> gmail.com (andrea conard) Date: Thu Oct 6 14:23:52 2011 Subject: [Histonet] open HT/HTL position Message-ID: HI all, I currently have an opening for a fulltime HT/HTL in Atlantic City. We work Monday through Friday days only, no holidays or weekends.If you know someone who is looking for a position in South Jersey please let them know. This is a modern lab doing aprox. 12K cases/year. I?ve been the supervisor for about one year and enjoy working at this facility a lot. If interested, please call me or answer this email. Andrea Conard, HT(ASCP) Pathology Supervisor AtlantiCare Regional Medical Center Phone: 609-441-8168Fax:609-441-2195 From rjbuesa <@t> yahoo.com Thu Oct 6 14:58:36 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 6 14:58:40 2011 Subject: [Histonet] "formol" In-Reply-To: <4C8F235CA0384093830B90BB552641EA@dielangs.at> Message-ID: <1317931116.44505.YahooMailClassic@web65705.mail.ac4.yahoo.com> ALL laboratories in Spain and ALL laboratories in Hispano America keep the German name for the 10% formaldehyde solution and call it "FORMOL". Ren? J. --- On Thu, 10/6/11, Gudrun Lang wrote: From: Gudrun Lang Subject: [Histonet] "formol" To: histonet@lists.utsouthwestern.edu Date: Thursday, October 6, 2011, 11:37 AM Formol is an old brandname, I think for a desinfection reagent. Not really custom any more. Bye (another) Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rene J Buesa Gesendet: Donnerstag, 06. Oktober 2011 15:13 An: histonet@lists.utsouthwestern.edu; Jenny Vega Betreff: [Histonet] Difference between Neutral Buffered Formalin 10% and NBformaldehyde 3.7 to 4% Jenny: Formaldehyde is?the GAS methanal?that dissolves in water at a maximum concentration between 37-40% vol./vol. To prevent its polymerization, small amounts of mathanol (an alcohol)are added. The 10% solution formaldehyde contains between 3.7 and 4.0% of formaldehyde and is called "formalin" in many countries and "formol" in Europe. So formalin is a 3.7 to 4.0% solution of formaldehyde. So a 10% formalin = 3.7-4.0% formaldehyde. NBF is prepared by added phosphate salts to prevent the formalin to acidify and is used to fix tissues. It is a pitty that your supervisor has so little knowledge about the area she is supposed to supervise, but I am not do not know why, but I am not.?surprised. To your question: SHE is wrong, really wrong! Ren? J. ? --- On Wed, 10/5/11, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 5, 2011, 7:03 PM Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it? we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to? putrefied if they are put in that solution. Am I wrong or is she wrong? thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Thu Oct 6 15:18:28 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Oct 6 15:18:36 2011 Subject: [Histonet] "formol" In-Reply-To: <1317931116.44505.YahooMailClassic@web65705.mail.ac4.yahoo.com> References: <4C8F235CA0384093830B90BB552641EA@dielangs.at> <1317931116.44505.YahooMailClassic@web65705.mail.ac4.yahoo.com> Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFB32@nmdamailsvr.nmda.ad.nmsu.edu> Not to put too fine a point on this interesting discussion (or "lecture series", as it were), I add a little quote from R.D. Lillie, Histopathologic Technic and Practical Histochemistry, 3rd ed, 1965: "The word 'formal' [sic], sometimes erroneously used as a synonym, refers properly to quite another substance, CH3O-Ch2-OCH3, dimethoxymethane." It matters not and I'm not even quite sure why I decided to take up the fight - what was the question??? From julie.hinsinger <@t> umontreal.ca Thu Oct 6 15:37:27 2011 From: julie.hinsinger <@t> umontreal.ca (Hinsinger Julie) Date: Thu Oct 6 15:37:56 2011 Subject: [Histonet] Antibodies (IHC) on sheep /goat models In-Reply-To: <1D0EFE168DB8504198FAA1AA5BA1A2B631235D8F@MAPIUDEM2.sim.umontreal.ca> References: <1D0EFE168DB8504198FAA1AA5BA1A2B631235D8F@MAPIUDEM2.sim.umontreal.ca> Message-ID: <608453C8-61A9-4070-B00A-60FCFD657052@umontreal.ca> Looking for anti-cytokine antibodies (dete cting IL-1?, IL-1?, IL-6, TNF-?, and TNF-?) and a HAM-56 macrophage marker that react in sheep /goat models. This immunohistochemical analysis would be performed on parafin-embed ded sections and may be outsourced if preferable . Any help/ recommendation would be much appreciated. From pbodig <@t> histoprep.com Thu Oct 6 15:54:09 2011 From: pbodig <@t> histoprep.com (Peter Bodig) Date: Thu Oct 6 15:54:19 2011 Subject: [Histonet] Poor quality frozen sections, feline skin. Message-ID: I have some feline skin samples for frozen sections that were sent to us because our client was not able to section them themselves. Unfortunately we are having the same problems trying to make good sections ourselves. The samples seem dry and unsupported when we try to cut them. The tissue breaks into small pieces and falls out or the OCT when we try to cut it. We have tried fixation with 10% NBF for various times up to 24 hrs prior to doing frozen sections to give the tissue better support, which helps, but not enough, and then the tissue falls off the coated slides when we try to stain them. Sectioning at 8? helps as well (that is as thick as out cryostat will go). Does anyone have suggestions on how to get good quality frozen sections from feline skin? We have come up with some questions as well: 1. Is there a way to support the tissue by infiltrating it with OCT or other similar media to improve our sections? 2. I have read about using 30% sucrose solution for cryo protection, does that aide in making good quality sections or just reduce freezing artifact? 3. Does tissue need to be fixed prior to using cryo protection? Our client will be using the sections for Senescence ?-Galactosidase Staining. Thank you for any help you can provide, P?ter Bodig Laboratory Coordinator Colorado Histo-Prep E-mail: pbodig@histoprep.com From rjbuesa <@t> yahoo.com Thu Oct 6 16:02:25 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 6 16:02:29 2011 Subject: [Histonet] "formol" In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DFB32@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1317934945.48431.YahooMailClassic@web65704.mail.ac4.yahoo.com> Formal is what Dr. Lilklie says, FORMOL is methanal (HCHO). The name started in Germany in 1891 as the trade name for the 37-40% aq. sol. of methanal or formaldehyde. It was adopted in Spain and all Hispano American countries after Dr. Santiago Ram?n y Cajal (Nobel Proze in Medicine along with Golgi) when he translated his silver methods to German. Please refer to: Fox, Johnson, Whiting and Roller (1985): Formaldehyde fixation. J.Histochem.Cytochem; 33(8):845-853 Ren? J. --- On Thu, 10/6/11, Breeden, Sara wrote: From: Breeden, Sara Subject: RE: [Histonet] "formol" To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, gu.lang@gmx.at Date: Thursday, October 6, 2011, 4:18 PM Not to put too fine a point on this interesting discussion (or "lecture series", as it were), I add a little quote from R.D. Lillie, Histopathologic Technic and Practical Histochemistry, 3rd ed, 1965: "The word 'formal' [sic], sometimes erroneously used as a synonym, refers properly to quite another substance, CH3O-Ch2-OCH3, dimethoxymethane." It matters not? and I'm not even quite sure why I decided to take up the fight -? what was the question??? From histotech411 <@t> gmail.com Thu Oct 6 18:03:28 2011 From: histotech411 <@t> gmail.com (Jenny Vega) Date: Thu Oct 6 18:03:31 2011 Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% In-Reply-To: <1317906775.25229.YahooMailClassic@web65703.mail.ac4.yahoo.com> References: <1317906775.25229.YahooMailClassic@web65703.mail.ac4.yahoo.com> Message-ID: Thanks. Thank God that the company that supplied the product contacted her and set her straight by telling her the same information you guys have told me. I even pointed the chapter from the Frieda Carson's book again, when it clearly says they are the same thing. Now she understood! Thank God I was getting frustrated because she did not agreed with me :(. On Thu, Oct 6, 2011 at 9:12 AM, Rene J Buesa wrote: > Jenny: > Formaldehyde is the GAS methanal that dissolves in water at a maximum > concentration between 37-40% vol./vol. To prevent its polymerization, small > amounts of mathanol (an alcohol)are added. > The 10% solution formaldehyde contains between 3.7 and 4.0% of formaldehyde > and is called "formalin" in many countries and "formol" in Europe. > So formalin is a 3.7 to 4.0% solution of formaldehyde. > So a 10% formalin = 3.7-4.0% formaldehyde. > NBF is prepared by added phosphate salts to prevent the formalin to acidify > and is used to fix tissues. > It is a pitty that your supervisor has so little knowledge about the area > she is supposed to supervise, but I am not do not know why, but I am > not. surprised. > To your question: SHE is wrong, really wrong! > Ren? J. > > > --- On *Wed, 10/5/11, Jenny Vega * wrote: > > > From: Jenny Vega > Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB > formaldehyde 3.7 to 4% > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, October 5, 2011, 7:03 PM > > Ok I want to know the diferrence between Neutral Buffered Formalin 10% and > NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same > thing and in the book from Frieda Carson it says that but my supervisor > swears they are different chemicals. In the laboratory she has used 10% > neutral buffered formalin all the time, but when we ran out of it we were > sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB > formaldehyde 3.7 to 4% once again. She says that 10% NBF is more > concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are > going to putrefied if they are put in that solution. > > Am I wrong or is she wrong? thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From julie.bilkey <@t> gmail.com Thu Oct 6 22:36:24 2011 From: julie.bilkey <@t> gmail.com (Julie Bilkey) Date: Thu Oct 6 22:36:30 2011 Subject: [Histonet] Poor quality frozen sections, feline skin. In-Reply-To: References: Message-ID: Hi Peter, We use 20-25% sucrose on our fresh specimens for immunofluorescence (IF). We only deal with human skin samples so I will not comment on feline skin. I sit the specimen in three changes of sucrose for 10 minutes each. Before embedding in the cryostat, I gently blot it on a tissue. My understanding is that the sucrose does give support like a matrix, and it has saved dried specimens for me in the past. I hope this helps. Julie Bilkey. Sydney Skin Pathology. On Fri, Oct 7, 2011 at 7:54 AM, Peter Bodig wrote: > I have some feline skin samples for frozen sections that were sent to us > because our client was not able to section them themselves. Unfortunately we > are having the same problems trying to make good sections ourselves. The > samples seem dry and unsupported when we try to cut them. The tissue breaks > into small pieces and falls out or the OCT when we try to cut it. We have > tried fixation with 10% NBF for various times up to 24 hrs prior to doing > frozen sections to give the tissue better support, which helps, but not > enough, and then the tissue falls off the coated slides when we try to stain > them. Sectioning at 8? helps as well (that is as thick as out cryostat will > go). > > > > Does anyone have suggestions on how to get good quality frozen sections > from feline skin? > > > > We have come up with some questions as well: > > > > 1. Is there a way to support the tissue by infiltrating it with OCT or > other similar media to improve our sections? > 2. I have read about using 30% sucrose solution for cryo protection, > does that aide in making good quality sections or just reduce freezing > artifact? > 3. Does tissue need to be fixed prior to using cryo protection? > > > > Our client will be using the sections for Senescence ?-Galactosidase > Staining. > > > > Thank you for any help you can provide, > > > > > > P?ter Bodig > > Laboratory Coordinator > > Colorado Histo-Prep > > E-mail: pbodig@histoprep.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mehlikafaire <@t> hotmail.com Thu Oct 6 23:09:16 2011 From: mehlikafaire <@t> hotmail.com (Mehlika Faire) Date: Thu Oct 6 23:09:22 2011 Subject: [Histonet] Poor quality frozen sections, feline skin. In-Reply-To: References: , Message-ID: We fix our tissues first in 4% PFA, wash it 3x 15min each with pbs, then place it directly onto 30% Sucrose to cryoprotect until it sinks -usually over night. I am not sure if the Sucrose helps in the quAlity of the sections, but I have dealt with issues similar to what you are dealing with, mostly with snap freeze or meoh/acetone fixed tissues without cryoprotectant. Hope this helps. -Mehlika > Date: Fri, 7 Oct 2011 14:36:24 +1100 > From: julie.bilkey@gmail.com > To: pbodig@histoprep.com > Subject: Re: [Histonet] Poor quality frozen sections, feline skin. > CC: histonet@lists.utsouthwestern.edu > > Hi Peter, > > We use 20-25% sucrose on our fresh specimens for immunofluorescence (IF). We > only deal with human skin samples so I will not comment on feline skin. I > sit the specimen in three changes of sucrose for 10 minutes each. Before > embedding in the cryostat, I gently blot it on a tissue. My understanding is > that the sucrose does give support like a matrix, and it has saved dried > specimens for me in the past. > > I hope this helps. > > Julie Bilkey. > Sydney Skin Pathology. > > On Fri, Oct 7, 2011 at 7:54 AM, Peter Bodig wrote: > > > I have some feline skin samples for frozen sections that were sent to us > > because our client was not able to section them themselves. Unfortunately we > > are having the same problems trying to make good sections ourselves. The > > samples seem dry and unsupported when we try to cut them. The tissue breaks > > into small pieces and falls out or the OCT when we try to cut it. We have > > tried fixation with 10% NBF for various times up to 24 hrs prior to doing > > frozen sections to give the tissue better support, which helps, but not > > enough, and then the tissue falls off the coated slides when we try to stain > > them. Sectioning at 8? helps as well (that is as thick as out cryostat will > > go). > > > > > > > > Does anyone have suggestions on how to get good quality frozen sections > > from feline skin? > > > > > > > > We have come up with some questions as well: > > > > > > > > 1. Is there a way to support the tissue by infiltrating it with OCT or > > other similar media to improve our sections? > > 2. I have read about using 30% sucrose solution for cryo protection, > > does that aide in making good quality sections or just reduce freezing > > artifact? > > 3. Does tissue need to be fixed prior to using cryo protection? > > > > > > > > Our client will be using the sections for Senescence ?-Galactosidase > > Staining. > > > > > > > > Thank you for any help you can provide, > > > > > > > > > > > > P?ter Bodig > > > > Laboratory Coordinator > > > > Colorado Histo-Prep > > > > E-mail: pbodig@histoprep.com > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Fri Oct 7 08:23:48 2011 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Oct 7 08:23:57 2011 Subject: [Histonet] frozen breast tissue Message-ID: <6DC87DEA9229894DB3A09F8B61717A46106F7C79@hc-mailboxc1-n3.healthcare.uiowa.edu> Hello everyone: I have a research project that wants to collect and study breast tissue both tumor and normal for long range studies (not clearly defined) that would include DNA/RNA/mRNA/protein expression along with many other possibilities. I would like your expertise (particularly from the researchers) as to what methods of preservation and storage you would recommend. Currently we have been snap freezing foil wrapped fresh tissue at -80 using a SnapFrost unit and then storing at -130 and also doing the same with a small OCT embedding piece of tissue as well. I'm concerned about the ability to cut frozen sections on the OCT specimen. Particularly, I'd be interested in opinions regarding any options for embedding and freezing fresh tissue that might enhance the ability to cut frozen sections rather than having to resort to FFPE. What are your thoughts and experiences? Thanks. Joe joseph-galbraith@uiowa.edu ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From mcauliff <@t> umdnj.edu Fri Oct 7 09:40:56 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Oct 7 09:40:17 2011 Subject: [Histonet] Imidazole buffer In-Reply-To: <4E8D7994.B440.007F.1@inspection.gc.ca> References: <4E8D7994.B440.007F.1@inspection.gc.ca> Message-ID: <4E8F0F78.1040107@umdnj.edu> Hi Tammy: Here are some references for imidazole in buffers: Straus, W., 1982. Imidazole increases the sensitivity of the cytochemical reaction for peroxidase with diaminobenzidine at a neutral pH. J. Histochem. Cytochem. 30:491-493. Trojanowski, J.Q., M.A. Obrocka and V. Lee. 1983. A comparison of eight different chromogen protocols for the demonstration of immunoreactive neurofilaments or glial filaments in rat cerebellum using the peroxidase-antiperoxidase method and monoclonal antibodies. J. Histochem. Cytochem. 31:1217-1223. Geoff On 10/6/2011 11:49 AM, Tammy Pickles wrote: > Hi, > > I am wondering if anyone out there can tell me the function of Imidazole, when used in a buffer. We are currently using it to make up our in house DAB solution. The results are fantastic with an overall increase in sensitivity with all of our antibodies. The only information I can find concerning the function is to help in blocking nonspecific staining. Does anyone have more information to share? > > Thanks in advance, > Tammy > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From JMacDonald <@t> mtsac.edu Fri Oct 7 11:35:35 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Oct 7 11:35:40 2011 Subject: [Histonet] Histotechnician requirements in Europe Message-ID: Does anyone know the education/certification/licensure requirements for histotechnicians in Europe? Thank you, Jennifer MacDonald From bpohl <@t> swedishamerican.org Fri Oct 7 12:07:08 2011 From: bpohl <@t> swedishamerican.org (Pohl, Brandon J) Date: Fri Oct 7 12:07:24 2011 Subject: [Histonet] Standardizing staining/IHC protocols by case or tissue type Message-ID: <1FFD981919A77641993CD2A75ED2C6966B2627@SAC3-WIN-SRV35.swedishamerican.org> I am having trouble getting my pathologists to understand that their lack of standardization or consistency in ordering special stains and IHC is resulting in such low volumes that many of the stains or antibodies are not cost effective for us to run. They seem to think that the way they each individually order certain stains based on preference or comfort level in reading is a norm amongst all pathologists. I am hoping to get some feedback from anyone willing in order to find out if they are correct or if my point of standardizing their methods is valid. For anyone willing to share, could you please let me know what types of protocols or panels you have established for different types of tissues or different types of cancers that are SOP for each of those cases. I know some cases require flexibility and variation, but we have very little in terms of standardization that each pathologist follows. Thanks! Brandon Pohl MAT, HTL (ASCP) Histology Supervisor Swedish American Hospital Rockford, IL 61104 815-489-4279 bpohl@swedishamerican.org From wbenton <@t> cua.md Fri Oct 7 12:33:28 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Fri Oct 7 12:33:34 2011 Subject: [Histonet] Standardizing staining/IHC protocols by case or tissue type In-Reply-To: <1FFD981919A77641993CD2A75ED2C6966B2627@SAC3-WIN-SRV35.swedishamerican.org> References: <1FFD981919A77641993CD2A75ED2C6966B2627@SAC3-WIN-SRV35.swedishamerican.org> Message-ID: <0B8979A204680A42B93A52B486088CD9206C204318@CUAEXH1.GCU-MD.local> Brandon, In my experience there generally isn't a set SOP for ordering patterns. However, there are exceptions for breast cases, melanoma cases etc..., but those are the minority, not the majority. However, you can approach the cost effective process with them. Given most antibodies have a relatively long shelf life 2-3 years, I would recommend running a report and determining how many times an antibody was ordered of the course of year. In many cases as I experienced new antibodies replaced older antibodies and thus were not being ordered. As a result we removed those antibodies from the antibody offering. You'll need to reach an agreed upon number of tests that need to be ordered, which remove any bias that may result from presenting the numbers prior to that being discussed. In addition, it may be beneficial for you to work with you reference lab that performs IHC or Specials that you routinely do not perform to get firm TAT that will entice the pathologist to utilize that source instead of in-house. I hope this helps. We had to do this when I managed a large university lab with well over 125 antibodies. In the end we eliminated around 20 or so and brought on 5-10 new antibodies that were being sent out frequently and ultimately were more cost effective in-house and had a better TAT in-house. Hope this helps! Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pohl, Brandon J [bpohl@swedishamerican.org] Sent: Friday, October 07, 2011 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Standardizing staining/IHC protocols by case or tissue type I am having trouble getting my pathologists to understand that their lack of standardization or consistency in ordering special stains and IHC is resulting in such low volumes that many of the stains or antibodies are not cost effective for us to run. They seem to think that the way they each individually order certain stains based on preference or comfort level in reading is a norm amongst all pathologists. I am hoping to get some feedback from anyone willing in order to find out if they are correct or if my point of standardizing their methods is valid. For anyone willing to share, could you please let me know what types of protocols or panels you have established for different types of tissues or different types of cancers that are SOP for each of those cases. I know some cases require flexibility and variation, but we have very little in terms of standardization that each pathologist follows. Thanks! Brandon Pohl MAT, HTL (ASCP) Histology Supervisor Swedish American Hospital Rockford, IL 61104 815-489-4279 bpohl@swedishamerican.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From afimbres <@t> uci.edu Fri Oct 7 13:05:13 2011 From: afimbres <@t> uci.edu (Fimbres, Amber) Date: Fri Oct 7 13:05:18 2011 Subject: [Histonet] Histotechnician requirements in Europe In-Reply-To: References: Message-ID: I only know about the UK's Institute for Biomedical Science. Check out http://www.ibms.org/go/registration for an explanation and guide on how to register because according to their website "all biomedical scientists wishing to practise in the UK under the protected title of biomedical scientist must be registered with the Health Professions Council (HPC)." Good luck, Amber Amber M. Fimbres, MHA, CT(ASCP)HTL Cytotechnologist UC Irvine Medical Center afimbres@uci.edu -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Friday, October 07, 2011 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechnician requirements in Europe Does anyone know the education/certification/licensure requirements for histotechnicians in Europe? Thank you, Jennifer MacDonald This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. From PMonfils <@t> Lifespan.org Fri Oct 7 14:53:18 2011 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Oct 7 14:53:43 2011 Subject: [Histonet] control for Hemoglobin? In-Reply-To: <869848DDBB7C5D4896A569A38B814E690551CAD1@CVMMB01.cvm.tamu.edu> References: <869848DDBB7C5D4896A569A38B814E690551CAD1@CVMMB01.cvm.tamu.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E08B4B5CC@LSRIEXCH1.lsmaster.lifespan.org> Just about any tissue has some small blood vessels with red cells in them. Seems like that should suffice as a control for hemoglobin. From PMonfils <@t> Lifespan.org Fri Oct 7 15:16:26 2011 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Oct 7 15:16:31 2011 Subject: [Histonet] Cold Plates - Source? Message-ID: <4EBFF65383B74D49995298C4976D1D5E08B4B5D3@LSRIEXCH1.lsmaster.lifespan.org> Hello All, Does anyone know where Tissue-Tek cold plates can be purchased? They are plastic, black on the bottom, white on top, gel filled, for chilling blocks. I got them from Cardinal Healthcare a couple of years ago, but I don't see it in their catalog now. Or, if no longer available, a comparable other product? I know I can use a pan of ice, but I prefer something like this if available. Thanks. From brannon <@t> alliedsearchpartners.com Fri Oct 7 15:18:05 2011 From: brannon <@t> alliedsearchpartners.com (Jonathan Owens) Date: Fri Oct 7 15:18:31 2011 Subject: [Histonet] Histotechnician or Histotechnologist needed in Spokane Valley, WA Message-ID: Allied Search Partners is currently looking for a Histology Professional to work in Spokane Valley. Position Title: Histotechnician or Histotechnologist Location: Spokane Valley, WA Schedule: Full Time/Permanent Summary and Requirements: 1 year+ experience with all aspects of Histology ASCP Preferred Benefits: Medical / Dental / Optical Insurance, Life Insurance, 401K Retirement, EAP Program, Tuition Reimbursement, Paid Holiday and PTO, Continuing Education, Personal Insurance To Apply: Please send resume to Brannon@alliedsearchpartners.com. If all qualifications are met then we will call to schedule a phone screen with one of our recruiters. -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From brannon <@t> alliedsearchpartners.com Fri Oct 7 15:19:24 2011 From: brannon <@t> alliedsearchpartners.com (Jonathan Owens) Date: Fri Oct 7 15:19:43 2011 Subject: [Histonet] Histotechnician or Histotechnologist needed in Walla Walla, WA Message-ID: Allied Search Partners is currently looking for a Histology Professional to work in Walla Walla. Position Title: Histotechnician or Histotechnologist Location: Walla Walla, WA Schedule: Full Time/Permanent Summary and Requirements: 1 year+ experience with all aspects of Histology ASCP Preferred Benefits: Medical / Dental / Optical Insurance, Life Insurance, 401K Retirement, EAP Program, Tuition Reimbursement, Paid Holiday and PTO, Continuing Education, Personal Insurance To Apply: Please send resume to Brannon@alliedsearchpartners.com. If all qualifications are met then we will call to schedule a phone screen with one of our recruiters. -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From brannon <@t> alliedsearchpartners.com Fri Oct 7 15:21:42 2011 From: brannon <@t> alliedsearchpartners.com (Jonathan Owens) Date: Fri Oct 7 15:21:50 2011 Subject: [Histonet] Histology Supervisor needed in Ft. Myers, FL Message-ID: Allied Search Partners is currently looking to hire Histology Supervisors for two Fort Myers based laboratories. Summary: Supervisor the daily routine and specialized histology techniques and procedures. Supervision of Embedding, Microtomy, Grossing, Processing, and H&E staining Special Staining and Equipment maintenance Requirements: FL Supervisor Laboratory License Previous experience with automated IHC Technical and QC protocols AA or BS/BA degree in life science preferred but not required Benefits: Competitive salaries, Health, Dental, Life & Disability insurances, a section 125 plan, a 401K, FSA, ESPP and relocation assistance. -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From rjbuesa <@t> yahoo.com Fri Oct 7 15:22:38 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 7 15:22:42 2011 Subject: [Histonet] Standardizing staining/IHC protocols by case or tissue type In-Reply-To: <1FFD981919A77641993CD2A75ED2C6966B2627@SAC3-WIN-SRV35.swedishamerican.org> Message-ID: <1318018958.17691.YahooMailClassic@web65711.mail.ac4.yahoo.com> You CANNOT establish a protocol based on tissue types. That is for starters. You have to standardize a general protocol, in the same way that you set your tissue processor with a "general protocol" that will be good enough to process any type of tissue. This means that your pathologists are right and that they order ANY procedure they think will help them to determine a?correct diagnosis. You have to develop a standard protocol for IHC good enough for ANY epitope detection. The only acceptable variable is the pH for HIER and the dilution of the antibody. Everything else should be standard. So you are wrong in your assumption and your pathologist is right (at least for once!). Ren? J. ? --- On Fri, 10/7/11, Pohl, Brandon J wrote: From: Pohl, Brandon J Subject: [Histonet] Standardizing staining/IHC protocols by case or tissue type To: histonet@lists.utsouthwestern.edu Date: Friday, October 7, 2011, 1:07 PM I am having trouble getting my pathologists to understand that their lack of standardization or consistency in ordering special stains and IHC is resulting in such low volumes that many of the stains or antibodies are not cost effective for us to run.? They seem to think that the way they each individually order certain stains based on preference or comfort level in reading is a norm amongst all pathologists.? I am hoping to get some feedback from anyone willing in order to find out if they are correct or if my point of standardizing their methods is valid.? For anyone willing to share, could you please let me know what types of protocols or panels you have established for different types of tissues or different types of cancers that are SOP for each of those cases.? I know some cases require flexibility and variation, but we have very little in terms of standardization that each pathologist follows.? Thanks! Brandon Pohl MAT, HTL (ASCP) Histology Supervisor Swedish American Hospital Rockford, IL 61104 815-489-4279 bpohl@swedishamerican.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Fri Oct 7 15:37:16 2011 From: jstaruk <@t> masshistology.com (jstaruk) Date: Fri Oct 7 15:37:15 2011 Subject: [Histonet] Cold Plates - Source? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E08B4B5D3@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E08B4B5D3@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <000001cc8530$e7af94f0$b70ebed0$@masshistology.com> I saw a few cases on Ebay recently. Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, October 07, 2011 4:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cold Plates - Source? Hello All, Does anyone know where Tissue-Tek cold plates can be purchased? They are plastic, black on the bottom, white on top, gel filled, for chilling blocks. I got them from Cardinal Healthcare a couple of years ago, but I don't see it in their catalog now. Or, if no longer available, a comparable other product? I know I can use a pan of ice, but I prefer something like this if available. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1831 / Virus Database: 2085/4543 - Release Date: 10/07/11 From BDeBrosse-Serra <@t> isisph.com Fri Oct 7 15:47:19 2011 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Fri Oct 7 15:47:42 2011 Subject: [Histonet] Histotechnician requirements in Europe In-Reply-To: References: Message-ID: <493CAA64F203E14E8823737B9EE0E25F091E9126D4@EXCHMB01.isis.local> I think it will greatly depend on the country. I received my certification in Switzerland, which actually is the licensure of Biology Laboratory technician. It was a three year apprenticeship (I believe it is four years now) with an accredited facility (mine was the Animal Pathology of the University of Bern). You will be taught on the job and go also to a technical school, that offers classes specifically for majoring in Biology (German, Economics, Biology, Math, Chemistry, Physics). At the end of the three years, you have to take a practical and theoretical exam. My major was histology and minor in Microbiology. If you flunk either the practical or the theoretical (this exam was three or four days long....), you won't be issued the license, but you have the chance to retake the portion you didn't pass. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, October 07, 2011 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechnician requirements in Europe Does anyone know the education/certification/licensure requirements for histotechnicians in Europe? Thank you, Jennifer MacDonald _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michelecarr10 <@t> yahoo.com Fri Oct 7 22:37:28 2011 From: michelecarr10 <@t> yahoo.com (Michele Carr) Date: Sat Oct 8 00:31:31 2011 Subject: [Histonet] Green counterstain problems Message-ID: <64235x5oo8km11wwbc6qwoom.1318045048777@email.android.com> I'm having the same problem, would be interested in what others say. T-Mobile, America's First Nationwide 4G Network rgrow@bmnet.com wrote: Good Morning Histonetters! I've been having a counterstain problem with my GMS and PAS-F.? Both stains work as they should until the green counterstain is applied.? I cannot get my tissue (control or patient) to uptake the counterstain.? I have varied time, temperature, even companies.? I have good controls that have been in use for years without problems, until now.? Only after an extended -10+ minutes,- can I get a blush of color in them.? I've even called the slide company to ask if there has been a change to the charge coating I'm open to suggestions. Thanks! Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN? 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any? dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Oct 8 04:01:52 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Oct 8 04:02:00 2011 Subject: AW: [Histonet] "formol" In-Reply-To: <1317934945.48431.YahooMailClassic@web65704.mail.ac4.yahoo.com> References: <4D14F0FC9316DD41972D5F03C070908B051DFB32@nmdamailsvr.nmda.ad.nmsu.edu> <1317934945.48431.YahooMailClassic@web65704.mail.ac4.yahoo.com> Message-ID: <16D6FCBC7D69479AB627D24A4C5477F7@dielangs.at> Since each nation has its own characteristics, it?s likely that Spanish speaking countries and others use ?formol?. In Austria I hardly hear ?formol? for formalin or formaldehyd, refering to the chemical. But there are distributors, that sell formaldehyd under the brandname Formol. ?Formalin? itself isn?t a really chemical correct name, but a proper name introduced somewhere, isn?t it? I think there is a North-South, East-West gradient in relation to the use of romanic languages in Europe. Perhaps someone can make a master thesis about this. ;-) As long as we speak about the same stuff, it doesn?t matter Best regards Gudrun _____ Von: Rene J Buesa [mailto:rjbuesa@yahoo.com] Gesendet: Donnerstag, 06. Oktober 2011 23:02 An: histonet@lists.utsouthwestern.edu; gu.lang@gmx.at; SaraBreeden Betreff: RE: [Histonet] "formol" Formal is what Dr. Lilklie says, FORMOL is methanal (HCHO). The name started in Germany in 1891 as the trade name for the 37-40% aq. sol. of methanal or formaldehyde. It was adopted in Spain and all Hispano American countries after Dr. Santiago Ram?n y Cajal (Nobel Proze in Medicine along with Golgi) when he translated his silver methods to German. Please refer to: Fox, Johnson, Whiting and Roller (1985): Formaldehyde fixation. J.Histochem.Cytochem; 33(8):845-853 Ren? J. --- On Thu, 10/6/11, Breeden, Sara wrote: From: Breeden, Sara Subject: RE: [Histonet] "formol" To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, gu.lang@gmx.at Date: Thursday, October 6, 2011, 4:18 PM Not to put too fine a point on this interesting discussion (or "lecture series", as it were), I add a little quote from R.D. Lillie, Histopathologic Technic and Practical Histochemistry, 3rd ed, 1965: "The word 'formal' [sic], sometimes erroneously used as a synonym, refers properly to quite another substance, CH3O-Ch2-OCH3, dimethoxymethane." It matters not and I'm not even quite sure why I decided to take up the fight - what was the question??? From gu.lang <@t> gmx.at Sat Oct 8 04:08:58 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Oct 8 04:09:03 2011 Subject: AW: [Histonet] Histotechnician requirements in Europe In-Reply-To: References: Message-ID: <09CDEE96150549A1BE43E9A4022CB37A@dielangs.at> Which country? For Austria you have to be a "Biomedizinische Analytikerin" = biomedical scientist, if you want to work in a clinical-histological lab. Research, biological, animal labs have other requirements. http://www.bmgfj.gv.at/cms/home/attachments/9/9/0/CH1168/CMS1203513413471/an erkennungdurchnostrifikation_ai.pdf This link goes to the Austrian requirements for not-EU-citizens. It's in German, but there's an email adress for further information. Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jennifer MacDonald Gesendet: Freitag, 07. Oktober 2011 18:36 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Histotechnician requirements in Europe Does anyone know the education/certification/licensure requirements for histotechnicians in Europe? Thank you, Jennifer MacDonald _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Sat Oct 8 06:51:49 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat Oct 8 06:52:01 2011 Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% In-Reply-To: References: Message-ID: Wow. Just Wow! :o( Sent from my iPhone On Oct 5, 2011, at 7:03 PM, Jenny Vega wrote: > Ok I want to know the diferrence between Neutral Buffered Formalin 10% and > NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same > thing and in the book from Frieda Carson it says that but my supervisor > swears they are different chemicals. In the laboratory she has used 10% > neutral buffered formalin all the time, but when we ran out of it we were > sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB > formaldehyde 3.7 to 4% once again. She says that 10% NBF is more > concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are > going to putrefied if they are put in that solution. > > Am I wrong or is she wrong? thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Sat Oct 8 08:22:10 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Oct 8 08:22:13 2011 Subject: [Histonet] "formol" In-Reply-To: <16D6FCBC7D69479AB627D24A4C5477F7@dielangs.at> Message-ID: <1318080130.86595.YahooMailClassic@web65708.mail.ac4.yahoo.com> "Formol" was an early German designation for the 37-40% aq. sol. of methanal ("formaldehyde") used, as I wrote early, by all histopathology labs in?Spain and Hispano America. The BRAND NAME of a German manufacturer for that same 37-40% formaldehyde solution was "FORMALIN" and that was the name adopted in the USA and most other countries. I wonder how "formalin" is called in France. In Italy I think it is also "formol". It would be interesting to get feed back from colleagues as to how "formalin" is called in their countries. It is always nice to find learn something new! Ren? J. --- On Sat, 10/8/11, Gudrun Lang wrote: From: Gudrun Lang Subject: AW: [Histonet] "formol" To: "'Rene J Buesa'" Cc: histonet@lists.utsouthwestern.edu Date: Saturday, October 8, 2011, 5:01 AM Since each nation has its own characteristics, it?s likely that Spanish speaking countries and others use ?formol?. In Austria I hardly hear ?formol? for formalin or formaldehyd, refering to the chemical. But there are distributors, that sell formaldehyd under the brandname Formol. ?Formalin? itself isn?t a really chemical correct name, but a proper name introduced somewhere, isn?t it? ? I think there is a North-South, East-West gradient in relation to the use of romanic languages in Europe . Perhaps someone can make a master thesis about this. ;-) As long as we speak about the same stuff, it doesn?t matter? ? Best regards Gudrun ? ? Von: Rene J Buesa [mailto:rjbuesa@yahoo.com] Gesendet: Donnerstag, 06. Oktober 2011 23:02 An: histonet@lists.utsouthwestern.edu; gu.lang@gmx.at ; SaraBreeden Betreff: RE: [Histonet] "formol" ? Formal is what Dr. Lilklie says, FORMOL is methanal (HCHO). The name started in Germany in 1891 as the trade name for the 37-40% aq. sol. of methanal or formaldehyde. It was adopted in Spain and all Hispano American countries after Dr. Santiago Ram?n y Cajal (Nobel Proze in Medicine along with Golgi) when he translated his silver methods to German. Please refer to: Fox, Johnson, Whiting and Roller (1985): Formaldehyde fixation. J.Histochem.Cytochem; 33(8):845-853 Ren? J. --- On Thu, 10/6/11, Breeden, Sara wrote: From: Breeden, Sara Subject: RE: [Histonet] "formol" To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, gu.lang@gmx.at Date: Thursday, October 6, 2011, 4:18 PM Not to put too fine a point on this interesting discussion (or "lecture series", as it were), I add a little quote from R.D. Lillie, Histopathologic Technic and Practical Histochemistry, 3rd ed, 1965: "The word 'formal' [sic], sometimes erroneously used as a synonym, refers properly to quite another substance, CH3O-Ch2-OCH3, dimethoxymethane." It matters not? and I'm not even quite sure why I decided to take up the fight -? what was the question??? ? From rjbuesa <@t> yahoo.com Sat Oct 8 08:23:48 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Oct 8 08:23:51 2011 Subject: [Histonet] Green counterstain problems In-Reply-To: <64235x5oo8km11wwbc6qwoom.1318045048777@email.android.com> Message-ID: <1318080228.16994.YahooMailClassic@web65706.mail.ac4.yahoo.com> I think that your leight green solutions is too diluted. Prepare a new solution and try again. Ren? J. --- On Fri, 10/7/11, Michele Carr wrote: From: Michele Carr Subject: Re: [Histonet] Green counterstain problems To: rgrow@bmnet.com, histonet@lists.utsouthwestern.edu Date: Friday, October 7, 2011, 11:37 PM I'm having the same problem, would be interested in what others say. T-Mobile, America's First Nationwide 4G Network rgrow@bmnet.com wrote: Good Morning Histonetters! I've been having a counterstain problem with my GMS and PAS-F.? Both stains work as they should until the green counterstain is applied.? I cannot get my tissue (control or patient) to uptake the counterstain.? I have varied time, temperature, even companies.? I have good controls that have been in use for years without problems, until now.? Only after an extended -10+ minutes,- can I get a blush of color in them.? I've even called the slide company to ask if there has been a change to the charge coating I'm open to suggestions. Thanks! Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN? 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any? dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Sat Oct 8 08:54:28 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sat Oct 8 08:54:31 2011 Subject: [Histonet] Cassette clamp (universal) for sale - universal w adapter Message-ID: <1318082068.64408.YahooMailNeo@web39422.mail.mud.yahoo.com> Hello to you! ? We have a universal Tissue Tek quick-release cassette clamp for paraffin microtomes for sale.? It's been lightly used, has a few scratches and is in good working condition, overall.? It comes with an additional adapter for older AO microtomes.? Photos available. ? It's one of my favorites as the release lever is TALL?so it?takes less hand strength and it has cut-away sections to get your fingers under the edge of a cassette when taking it out of the clamp. ? Best reasonable offer (new runs $700-800 ea.) ? Cheryl Cheryl Kerry, HT(ASCP) 281.852.9457 tkngflght@yahoo.com From turkekul <@t> gmail.com Sat Oct 8 18:53:22 2011 From: turkekul <@t> gmail.com (Mesruh Turkekul) Date: Sat Oct 8 18:53:31 2011 Subject: [Histonet] 10% NBF vs. 3.7-4% formaldehyde Message-ID: <93C1EB19-2658-4C40-87C8-F1688BB25332@gmail.com> Concentrated formaldehyde solution (37-40%) can be diluted in water, buffer, alcohol etc. to prepare different fixatives. One example is 10% NBF. When concentrated formaldehyde is diluted 1:10 in buffer (usually sodium phosphate) with close to neutral pH and some preservatives and stabilizers added 10% NBF is obtained. Vendors always add methanol as a stabilizer. 10% NBF contains 3.7-4% formaldehyde among other ingredients. So 10% NBF is not the same thing as 3.7-4%formaldehyde. Whether they can be used interchangeably is open to discussion and has to be validated by the end user for their purposes. Sent from my iPadthy From jshea121 <@t> roadrunner.com Sun Oct 9 19:14:28 2011 From: jshea121 <@t> roadrunner.com (Shea's) Date: Sun Oct 9 19:14:32 2011 Subject: [Histonet] Green counterstain problems Message-ID: <4ECF7C3CEF464FD79473F0B6436C7B00@JoannePC> This happens to us after extended use. Add about 5 drops/100ml of glacial acetic acid to the light green working solution. From sccrshlly <@t> yahoo.com Sun Oct 9 22:38:53 2011 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Sun Oct 9 22:38:57 2011 Subject: [Histonet] Leaking Jung XL stainer Message-ID: <1318217933.71551.YahooMailNeo@web113720.mail.gq1.yahoo.com> I have a refurbished Jung XL autostainer that has been great for the past 5 years.? Last week the fan seized up and is being replaced.? While moving around the machine, I believe that inadvertently we somehow did something to cause the water hose to start leaking.? We tried getting a new hose, but it is still leaking.? Any suggestions? ? Thanks in advance! From TMcNemar <@t> lmhealth.org Mon Oct 10 05:13:50 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Oct 10 05:14:01 2011 Subject: [Histonet] RE: Cold Plates - Source? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E08B4B5D3@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E08B4B5D3@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: Check out the Histo-Cool trays http://www.marketlabinc.com/product.asp?P_ID=4854 You can get them from several places. They stay cold all day. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, October 07, 2011 4:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cold Plates - Source? Hello All, Does anyone know where Tissue-Tek cold plates can be purchased? They are plastic, black on the bottom, white on top, gel filled, for chilling blocks. I got them from Cardinal Healthcare a couple of years ago, but I don't see it in their catalog now. Or, if no longer available, a comparable other product? I know I can use a pan of ice, but I prefer something like this if available. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Valerie.Hannen <@t> parrishmed.com Mon Oct 10 04:59:50 2011 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Oct 10 05:43:17 2011 Subject: [Histonet] RE: Cold Plates - Source? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E08B4B5D3@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <450B7A81EDA0C54E97C53D60F00776C322A4AC0F29@isexstore03> You can still get them from Cardinal Health. The catalog number should be M7410-10. There are 6 to a pack. Hope this helps. Valerie Hannen, MLT(ASCP), HTL,SU(FL) Parrish Medical Center Titusville, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, October 07, 2011 4:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cold Plates - Source? Hello All, Does anyone know where Tissue-Tek cold plates can be purchased? They are plastic, black on the bottom, white on top, gel filled, for chilling blocks. I got them from Cardinal Healthcare a couple of years ago, but I don't see it in their catalog now. Or, if no longer available, a comparable other product? I know I can use a pan of ice, but I prefer something like this if available. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From jluis.palazon <@t> icman.csic.es Mon Oct 10 06:34:09 2011 From: jluis.palazon <@t> icman.csic.es (Jose Luis Palazon Fernandez) Date: Mon Oct 10 06:34:13 2011 Subject: [Histonet] EDTA Message-ID: <146077420.19002.1318246449245.JavaMail.tomcat@cedric> Dear List members Greetings I would like to ask you some questions about the use of EDTA as demineratizing agent. I have to do histology and histochemistry (including metallothioneins) in fish larvae preserved in Bouins solution. 1) First of all I would like to know what is the best formula (or the one you recomend) to prepare the EDTA solution, as well as the importance of maintaining a given pH and if it is necessary to put the samples in the refrigerator during the demineralization process. 2) Is EDTA recomended only for certain fixatives, or It can be used with any fixative, including Bouins 3) When using Bouins solution as fixative, many books recommend not to wash in water but instaed to remove the excess of picric acid using several washes in etanol 70%, on the other hand, I?ve read that EDTA precipitates in the presence of alcohols. What do you recomend in this case? 4) Do you think that the demineralization procedure could alter or affect the detection of metallothioneins in the tissues? Many thanks in advance Best regards Jos? Luis Dr. Jos? Luis Palaz?n Fern?ndez Instituto de Investigaciones Cient?ficas Universidad de Oriente Boca del Rio-Isla Margarita-Venezuela Direccion actual: Instituto de Ciencias Marinas de Andalucia-CSIC Campus universitario Rio San Pedro, 11510, Puerto Real, C?diz, Espa?a email: jluis.palazon@icman.csic.es; jose.palazon@ne.udo.edu.ve tlf: +34-956832612; fax: +34-956834701; cell: +34-600487100 From nunut86 <@t> hotmail.com Mon Oct 10 08:43:48 2011 From: nunut86 <@t> hotmail.com (Nat Nunut) Date: Mon Oct 10 08:43:51 2011 Subject: [Histonet] (no subject) Message-ID: Hi everybody! I'm interested in making a Prussian Blue (Potassium ferrocyanide) staining to highlight micro vascular infarctus in human brains. In the protocol I have, it's written that a nitric acid pre-treatment is necessary. I would like to know: 1) What nitric acid is used for in histological staining? 2) What is its function in histological staining? I hope you can help me. Thanks Natalia From Carmen.M.Garcia <@t> uv.es Mon Oct 10 09:10:25 2011 From: Carmen.M.Garcia <@t> uv.es (Carmen Maria Garcia Pascual) Date: Mon Oct 10 09:10:36 2011 Subject: [Histonet] ovarian cryosections In-Reply-To: <9186875781carmaga6@uv.es> References: <9186875781carmaga6@uv.es> Message-ID: <5355778534carmaga6@uv.es> Hello everybody: I am here requesting your help another time ;) I want to make ovarian cryosections and I want to know if there is any problem if I leave the ovaries in 30% sucrose at 4?C overnigth. Thank you very much. Kind regards, Carmen From rjbuesa <@t> yahoo.com Mon Oct 10 09:11:08 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 10 09:11:15 2011 Subject: [Histonet] Leaking Jung XL stainer In-Reply-To: <1318217933.71551.YahooMailNeo@web113720.mail.gq1.yahoo.com> Message-ID: <1318255868.96684.YahooMailClassic@web65707.mail.ac4.yahoo.com> Check the connection from the hose into de stainer. It may be broken/stressed. Ren? J. --- On Sun, 10/9/11, Shelly Coker wrote: From: Shelly Coker Subject: [Histonet] Leaking Jung XL stainer To: "histonet@lists.utsouthwestern.edu" Date: Sunday, October 9, 2011, 11:38 PM I have a refurbished Jung XL autostainer that has been great for the past 5 years.? Last week the fan seized up and is being replaced.? While moving around the machine, I believe that inadvertently we somehow did something to cause the water hose to start leaking.? We tried getting a new hose, but it is still leaking.? Any suggestions? ? Thanks in advance! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Mon Oct 10 09:20:21 2011 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Oct 10 09:20:25 2011 Subject: [Histonet] Leaking Jung XL stainer In-Reply-To: <1318255868.96684.YahooMailClassic@web65707.mail.ac4.yahoo.com> References: <1318217933.71551.YahooMailNeo@web113720.mail.gq1.yahoo.com> <1318255868.96684.YahooMailClassic@web65707.mail.ac4.yahoo.com> Message-ID: <1318256421.4244.YahooMailNeo@web1116.biz.mail.sk1.yahoo.com> Hi, ? as stainers age, the hoses can harden and crack. I have replaced all hoses on many. ? If one is bad, they all are. By replacing one, you may have put stress on the next. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Rene J Buesa To: "histonet@lists.utsouthwestern.edu" ; Shelly Coker Sent: Monday, October 10, 2011 9:11 AM Subject: Re: [Histonet] Leaking Jung XL stainer Check the connection from the hose into de stainer. It may be broken/stressed. Ren? J. --- On Sun, 10/9/11, Shelly Coker wrote: From: Shelly Coker Subject: [Histonet] Leaking Jung XL stainer To: "histonet@lists.utsouthwestern.edu" Date: Sunday, October 9, 2011, 11:38 PM I have a refurbished Jung XL autostainer that has been great for the past 5 years.? Last week the fan seized up and is being replaced.? While moving around the machine, I believe that inadvertently we somehow did something to cause the water hose to start leaking.? We tried getting a new hose, but it is still leaking.? Any suggestions? ? Thanks in advance! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sarah_taba <@t> yahoo.com Mon Oct 10 12:37:02 2011 From: sarah_taba <@t> yahoo.com (sarah Tabatabaei) Date: Mon Oct 10 12:37:05 2011 Subject: [Histonet] 10% sucorse in Sodium Cacodylate In-Reply-To: <1318255868.96684.YahooMailClassic@web65707.mail.ac4.yahoo.com> References: <1318217933.71551.YahooMailNeo@web113720.mail.gq1.yahoo.com> <1318255868.96684.YahooMailClassic@web65707.mail.ac4.yahoo.com> Message-ID: <1318268222.6789.YahooMailNeo@web45710.mail.sp1.yahoo.com> Hi histoneters, I have a question regarding Sodium Cacodylate. In our lab, after fixing our human tissue, we usually leave it in 10% sucrose in double distilled water for a couple of days to remove all the fixatives out of it. Recently, I? used a solution of 10% sucrose in 0.1 ml Sodium Cacodylate, instead of the plain distilled water thing. I have a feeling I have ruined them for IHC. right? Can someone tell me what I have done to my tissue? I am supposed to do immunohistochemistry and some histology on these tissues. Looking forward to hear from you Sarah From Eric.Hoy <@t> UTSouthwestern.edu Mon Oct 10 12:45:59 2011 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Mon Oct 10 12:46:05 2011 Subject: [Histonet] Histotechnologist PRN Message-ID: Hello Histonetters! I am looking for a histotechnologist in the Dallas, TX area to assist with a project. The project will involve cutting serial frozen sections of rodent tissues for immunofluorescent analysis. The work will be on an ?as needed? basis, and the hours can be very flexible. If you are interested, please contact me off-list at: Eric.Hoy@UTSouthwestern.edu =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== From xu.chen <@t> maxvisionbio.com Mon Oct 10 13:03:54 2011 From: xu.chen <@t> maxvisionbio.com (Xu Chen) Date: Mon Oct 10 13:03:59 2011 Subject: [Histonet] coverslip for in-situ hybridization Message-ID: <006d01cc8776$f9895740$ec9c05c0$@maxvisionbio.com> Hello, Histoneter, Do you mind to tell me where are you buying coverslip for in-situ hybridization probe incubation? What's the catalogue number? Thank you very much for sharing your information. Best, Xu From rgrow <@t> bmnet.com Mon Oct 10 14:00:35 2011 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Mon Oct 10 14:00:39 2011 Subject: [Histonet] Re: Green Counterstain Problems In-Reply-To: References: Message-ID: Many thanks to all who responded to the green counterstain problem. It was the working solution that was bought premade that I had problems with. The addition of glacial acetic acid fixed my problem. Again, thank you Histonet! Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax Message: 1 Date: Sun, 9 Oct 2011 20:14:28 -0400 From: "Shea's" Subject: [Histonet] Green counterstain problems To: Message-ID: <4ECF7C3CEF464FD79473F0B6436C7B00@JoannePC> Content-Type: text/plain; charset="iso-8859-1" This happens to us after extended use. Add about 5 drops/100ml of glacial acetic acid to the light green working solution. From Dhanya.Menon <@t> petermac.org Mon Oct 10 17:12:36 2011 From: Dhanya.Menon <@t> petermac.org (Menon Dhanya) Date: Mon Oct 10 17:17:49 2011 Subject: [Histonet] RE:Leaking Jung XL stainer In-Reply-To: <19740412040257.ADF85D9A3781A2C0@mail1.petermac.org> References: <19740412040257.ADF85D9A3781A2C0@mail1.petermac.org> Message-ID: <6D3E01C097DB5C479B25F4063F6385CB06802D3C@PMC-EMAIL.petermac.org.au> Hello, We had a similar problem with our jung autostainer Xl after we had it serviced, but was fixed after we adjusted the water pressure from the tap....hope this helps 2010 Metropolitan Health Service of the Year -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, 11 October 2011 4:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 95, Issue 14 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Green counterstain problems (Shea's) 2. Leaking Jung XL stainer (Shelly Coker) 3. RE: Cold Plates - Source? (Tom McNemar) 4. RE: Cold Plates - Source? (Hannen, Valerie) 5. EDTA (Jose Luis Palazon Fernandez) 6. (no subject) (Nat Nunut) 7. ovarian cryosections (Carmen Maria Garcia Pascual) 8. Re: Leaking Jung XL stainer (Rene J Buesa) 9. Re: Leaking Jung XL stainer (Paula Pierce) ---------------------------------------------------------------------- Message: 1 Date: Sun, 9 Oct 2011 20:14:28 -0400 From: "Shea's" Subject: [Histonet] Green counterstain problems To: Message-ID: <4ECF7C3CEF464FD79473F0B6436C7B00@JoannePC> Content-Type: text/plain; charset="iso-8859-1" This happens to us after extended use. Add about 5 drops/100ml of glacial acetic acid to the light green working solution. ------------------------------ Message: 2 Date: Sun, 9 Oct 2011 20:38:53 -0700 (PDT) From: Shelly Coker Subject: [Histonet] Leaking Jung XL stainer To: "histonet@lists.utsouthwestern.edu" Message-ID: <1318217933.71551.YahooMailNeo@web113720.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I have a refurbished Jung XL autostainer that has been great for the past 5 years.? Last week the fan seized up and is being replaced.? While moving around the machine, I believe that inadvertently we somehow did something to cause the water hose to start leaking.? We tried getting a new hose, but it is still leaking.? Any suggestions? ? Thanks in advance! ------------------------------ Message: 3 Date: Mon, 10 Oct 2011 06:13:50 -0400 From: Tom McNemar Subject: [Histonet] RE: Cold Plates - Source? To: "'Monfils, Paul'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Check out the Histo-Cool trays http://www.marketlabinc.com/product.asp?P_ID=4854 You can get them from several places. They stay cold all day. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, October 07, 2011 4:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cold Plates - Source? Hello All, Does anyone know where Tissue-Tek cold plates can be purchased? They are plastic, black on the bottom, white on top, gel filled, for chilling blocks. I got them from Cardinal Healthcare a couple of years ago, but I don't see it in their catalog now. Or, if no longer available, a comparable other product? I know I can use a pan of ice, but I prefer something like this if available. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 4 Date: Mon, 10 Oct 2011 05:59:50 -0400 From: "Hannen, Valerie" Subject: [Histonet] RE: Cold Plates - Source? To: "'Monfils, Paul'" , "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C322A4AC0F29@isexstore03> Content-Type: text/plain; charset="us-ascii" You can still get them from Cardinal Health. The catalog number should be M7410-10. There are 6 to a pack. Hope this helps. Valerie Hannen, MLT(ASCP), HTL,SU(FL) Parrish Medical Center Titusville, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, October 07, 2011 4:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cold Plates - Source? Hello All, Does anyone know where Tissue-Tek cold plates can be purchased? They are plastic, black on the bottom, white on top, gel filled, for chilling blocks. I got them from Cardinal Healthcare a couple of years ago, but I don't see it in their catalog now. Or, if no longer available, a comparable other product? I know I can use a pan of ice, but I prefer something like this if available. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** ------------------------------ Message: 5 Date: Mon, 10 Oct 2011 13:34:09 +0200 (CEST) From: Jose Luis Palazon Fernandez Subject: [Histonet] EDTA To: histonet@lists.utsouthwestern.edu Message-ID: <146077420.19002.1318246449245.JavaMail.tomcat@cedric> Content-Type: text/plain; charset=iso-8859-1 Dear List members Greetings I would like to ask you some questions about the use of EDTA as demineratizing agent. I have to do histology and histochemistry (including metallothioneins) in fish larvae preserved in Bouins solution. 1) First of all I would like to know what is the best formula (or the one you recomend) to prepare the EDTA solution, as well as the importance of maintaining a given pH and if it is necessary to put the samples in the refrigerator during the demineralization process. 2) Is EDTA recomended only for certain fixatives, or It can be used with any fixative, including Bouins 3) When using Bouins solution as fixative, many books recommend not to wash in water but instaed to remove the excess of picric acid using several washes in etanol 70%, on the other hand, I?ve read that EDTA precipitates in the presence of alcohols. What do you recomend in this case? 4) Do you think that the demineralization procedure could alter or affect the detection of metallothioneins in the tissues? Many thanks in advance Best regards Jos? Luis Dr. Jos? Luis Palaz?n Fern?ndez Instituto de Investigaciones Cient?ficas Universidad de Oriente Boca del Rio-Isla Margarita-Venezuela Direccion actual: Instituto de Ciencias Marinas de Andalucia-CSIC Campus universitario Rio San Pedro, 11510, Puerto Real, C?diz, Espa?a email: jluis.palazon@icman.csic.es; jose.palazon@ne.udo.edu.ve tlf: +34-956832612; fax: +34-956834701; cell: +34-600487100 ------------------------------ Message: 6 Date: Mon, 10 Oct 2011 13:43:48 +0000 From: Nat Nunut Subject: [Histonet] (no subject) To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi everybody! I'm interested in making a Prussian Blue (Potassium ferrocyanide) staining to highlight micro vascular infarctus in human brains. In the protocol I have, it's written that a nitric acid pre-treatment is necessary. I would like to know: 1) What nitric acid is used for in histological staining? 2) What is its function in histological staining? I hope you can help me. Thanks Natalia ------------------------------ Message: 7 Date: Mon, 10 Oct 2011 16:10:25 +0200 (CEST) From: "Carmen Maria Garcia Pascual" Subject: [Histonet] ovarian cryosections To: histonet@lists.utsouthwestern.edu Message-ID: <5355778534carmaga6@uv.es> Content-Type: text/plain; charset="ISO-8859-1" Hello everybody: I am here requesting your help another time ;) I want to make ovarian cryosections and I want to know if there is any problem if I leave the ovaries in 30% sucrose at 4?C overnigth. Thank you very much. Kind regards, Carmen ------------------------------ Message: 8 Date: Mon, 10 Oct 2011 07:11:08 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Leaking Jung XL stainer To: "histonet@lists.utsouthwestern.edu" , Shelly Coker Message-ID: <1318255868.96684.YahooMailClassic@web65707.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Check the connection from the hose into de stainer. It may be broken/stressed. Ren? J. --- On Sun, 10/9/11, Shelly Coker wrote: From: Shelly Coker Subject: [Histonet] Leaking Jung XL stainer To: "histonet@lists.utsouthwestern.edu" Date: Sunday, October 9, 2011, 11:38 PM I have a refurbished Jung XL autostainer that has been great for the past 5 years.? Last week the fan seized up and is being replaced.? While moving around the machine, I believe that inadvertently we somehow did something to cause the water hose to start leaking.? We tried getting a new hose, but it is still leaking.? Any suggestions? ? Thanks in advance! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 10 Oct 2011 07:20:21 -0700 (PDT) From: Paula Pierce Subject: Re: [Histonet] Leaking Jung XL stainer To: Histonet Message-ID: <1318256421.4244.YahooMailNeo@web1116.biz.mail.sk1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi, ? as stainers age, the hoses can harden and crack. I have replaced all hoses on many. ? If one is bad, they all are. By replacing one, you may have put stress on the next. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Rene J Buesa To: "histonet@lists.utsouthwestern.edu" ; Shelly Coker Sent: Monday, October 10, 2011 9:11 AM Subject: Re: [Histonet] Leaking Jung XL stainer Check the connection from the hose into de stainer. It may be broken/stressed. Ren? J. --- On Sun, 10/9/11, Shelly Coker wrote: From: Shelly Coker Subject: [Histonet] Leaking Jung XL stainer To: "histonet@lists.utsouthwestern.edu" Date: Sunday, October 9, 2011, 11:38 PM I have a refurbished Jung XL autostainer that has been great for the past 5 years.? Last week the fan seized up and is being replaced.? While moving around the machine, I believe that inadvertently we somehow did something to cause the water hose to start leaking.? We tried getting a new hose, but it is still leaking.? Any suggestions? ? Thanks in advance! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 95, Issue 14 **************************************** This email (including any attachments or links) may contain confidential and/or legally privileged information and is intended only to be read or used by the addressee. If you are not the intended addressee, any use, distribution, disclosure or copying of this email is strictly prohibited. Confidentiality and legal privilege attached to this email (including any attachments) are not waived or lost by reason of its mistaken delivery to you. If you have received this email in error, please delete it and notify us immediately by telephone or email. Peter MacCallum Cancer Centre provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered and will not be liable for any delay in its receipt. From AnthonyH <@t> chw.edu.au Mon Oct 10 17:36:11 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Oct 10 17:36:31 2011 Subject: [Histonet] EDTA In-Reply-To: <146077420.19002.1318246449245.JavaMail.tomcat@cedric> References: <146077420.19002.1318246449245.JavaMail.tomcat@cedric> Message-ID: <6D6BD1DE8A5571489398B392A38A7157188A86DF@xmdb02.nch.kids> Jos?, I have inserted my ideas below within your email. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jose Luis Palazon Fernandez Sent: Monday, 10 October 2011 10:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EDTA Dear List members Greetings > I would like to ask you some questions about the use of EDTA as demineratizing agent. > I have to do histology and histochemistry (including metallothioneins) in fish larvae preserved in Bouins solution. How long do you fix in Bouins solution? Bouins, being an acidic fixative (picric and ascetic acids) will fix as well as decalcify, especially fish larvae which would have little calcium compared to human trephine specimens. So I am not sure whether you will need to decalcify. > 1) First of all I would like to know what is the best formula (or the one you recomend) to prepare the EDTA solution, as well as the importance of maintaining a given pH and if it is necessary to put the > > samples in the refrigerator during the demineralization process. The following solution was used by Sanderson et al (Biotech & Histochem 70(1):12-18, 1995): 1. To 1 litre of distilled water add 90ml concentrated ammonium hydroxide. 2. Stirring continuously, slowly add 140g EDTA 3. Adjust to pH 7.1 using concentrated ammonia. Adjustment of the pH is essential. If the pH is too low it works only as a too-weak acid. If the pH is too high (above 8) decalcification is accelerated but alkalinity can be damaging to tissues. > 2) Is EDTA recomended only for certain fixatives, or It can be used with any fixative, including Bouins Again why do you need to decalcify these specimens, since Bouins will also decalcify > 3) When using Bouins solution as fixative, many books recommend not to wash in water but instaed to remove the excess of picric acid using several washes in etanol 70%, on the other hand, I?ve read > that EDTA precipitates in the presence of alcohols. What do you recomend in this case? I also recommend washing in 70% ethanol since protein picrates are believed to be water soluble. I would suspect placing the Bouins fixed tissues in an aqueous EDTA solution would do the same thing (ie allow extraction of the picrates). > 4) Do you think that the demineralization procedure could alter or affect the detection of metallothioneins in the tissues? It would be possible that the Bouin's fixative might affect the antigens recognised by your metallothionein antibodies but unlikely that EDTA treatment would - only by testing would it become clearer. Are your antigens affected by Bouins? Many thanks in advance Best regards Jos? Luis Dr. Jos? Luis Palaz?n Fern?ndez Instituto de Investigaciones Cient?ficas Universidad de Oriente Boca del Rio-Isla Margarita-Venezuela Direccion actual: Instituto de Ciencias Marinas de Andalucia-CSIC Campus universitario Rio San Pedro, 11510, Puerto Real, C?diz, Espa?a email: jluis.palazon@icman.csic.es; jose.palazon@ne.udo.edu.ve tlf: +34-956832612; fax: +34-956834701; cell: +34-600487100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From AnthonyH <@t> chw.edu.au Mon Oct 10 17:40:55 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Oct 10 17:41:08 2011 Subject: [Histonet] ovarian cryosections In-Reply-To: <5355778534carmaga6@uv.es> References: <9186875781carmaga6@uv.es> <5355778534carmaga6@uv.es> Message-ID: <6D6BD1DE8A5571489398B392A38A7157188A8718@xmdb02.nch.kids> Carmen, If the tissues have been fixed in formalin, then this would not be a problem, but if not, why not freeze the tissues in OCT (or similar) and store it at -70oC before cryotomy, rather than placing it in a sucrose solution? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carmen Maria Garcia Pascual Sent: Tuesday, 11 October 2011 1:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ovarian cryosections Hello everybody: I am here requesting your help another time ;) I want to make ovarian cryosections and I want to know if there is any problem if I leave the ovaries in 30% sucrose at 4?C overnigth. Thank you very much. Kind regards, Carmen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Carmen.M.Garcia <@t> uv.es Tue Oct 11 03:43:16 2011 From: Carmen.M.Garcia <@t> uv.es (Carmen Maria Garcia Pascual) Date: Tue Oct 11 03:43:25 2011 Subject: [Histonet] Whole ovary immuno In-Reply-To: <5355778534carmaga6@uv.es> References: <5355778534carmaga6@uv.es> Message-ID: <1957774719carmaga6@uv.es> Good morning: I want to perfom an immunohistochemistry with fluorescence in a mice whole ovary, but I am new in this and in my lab there is nobody with experience in that. If someone has some protocol that I can follow, I would appreciate it. Thank you very much!!!! kind regards from Spain ;) Carmen ******************************************************* Carmen Mar?a Garc?a Pascual FIVI/INCLIVA/Facultad de Medicina Universidad de Valencia Departamento de P.O.G (Laboratorios) 96.386.40.48 Avd. Blasco Iba?ez 17 46010, Valencia ******************************************************* From ArtimK <@t> slhn.org Tue Oct 11 08:57:42 2011 From: ArtimK <@t> slhn.org (Artim, Kimberly) Date: Tue Oct 11 08:57:49 2011 Subject: [Histonet] Drying oven temps Message-ID: <9E67FDD215B226448638018A82B952BD026A16CCA401@EXCHANGE.slhn.org> Is anyone willing to share their slide drying temperature and times? Does anyone know of a particular standard concerning this? Kimberly Artim, AST, HT (ASCP) Technical Coordinator, Anatomic Pathology St Lukes Hospital & Health Network 801 Ostrum Street Bethlehem, PA 18015 610-954-4832 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mcauliff <@t> umdnj.edu Tue Oct 11 09:06:12 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Oct 11 09:05:35 2011 Subject: [Histonet] 10% sucorse in Sodium Cacodylate In-Reply-To: <1318268222.6789.YahooMailNeo@web45710.mail.sp1.yahoo.com> References: <1318217933.71551.YahooMailNeo@web113720.mail.gq1.yahoo.com> <1318255868.96684.YahooMailClassic@web65707.mail.ac4.yahoo.com> <1318268222.6789.YahooMailNeo@web45710.mail.sp1.yahoo.com> Message-ID: <4E944D54.3000405@umdnj.edu> On 10/10/2011 1:37 PM, sarah Tabatabaei wrote: > Hi histoneters, > > I have a question regarding Sodium Cacodylate. > In our lab, after fixing our human tissue, we usually leave it in 10% sucrose in double distilled water for a couple of days to remove all the fixatives out of it. Recently, I used a solution of 10% sucrose in 0.1 ml Sodium Cacodylate, instead of the plain distilled water thing. I have a feeling I have ruined them for IHC. right? Can someone tell me what I have done to my tissue? I am supposed to do immunohistochemistry and some histology on these tissues. > > > Looking forward to hear from you > > > Sarah > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Why do you think the specimens are ruined? Certainly no problem with the histology, cacodylate buffer has been used for many years for EM. Its use has declined as less-toxic alternatives have been found. As for immuno, try it, what have you got to lose? And why did you use cacodylate in the first place? Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Carmen.M.Garcia <@t> uv.es Tue Oct 11 10:11:26 2011 From: Carmen.M.Garcia <@t> uv.es (Carmen Maria Garcia Pascual) Date: Tue Oct 11 10:11:35 2011 Subject: [Histonet] Thick cryosections Message-ID: <7922198191carmaga6@uv.es> Good afternoon everyone! I have made 50um mice ovarian cryosections and I have used superfrost plus slides. But some of them fall of the slides when I perfomed the immuno, one colleague has told me that this is because the cuts are too thick, and I think she is rigth. I don't know if there is some thing that I can make to avoid this, like using another kind of slides... Thanks!!!!!!!!!!! From jpgrock37 <@t> yahoo.com Tue Oct 11 10:45:37 2011 From: jpgrock37 <@t> yahoo.com (Jessica Piche) Date: Tue Oct 11 10:45:42 2011 Subject: [Histonet] Cold plate temperatures on embedding station Message-ID: <1318347937.89323.YahooMailNeo@web121501.mail.ne1.yahoo.com> Hello All, ? We just got the Thermo Histostar. Curious to know if there is a CAP requirement for taking the cold plate temperatures on an embedding station? Need to know for quality control purposes. Thank you. ? Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital From liz <@t> premierlab.com Tue Oct 11 10:48:32 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Oct 11 10:49:59 2011 Subject: [Histonet] Cold plate temperatures on embedding station In-Reply-To: <1318347937.89323.YahooMailNeo@web121501.mail.ne1.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA0B83@SBS2K8.premierlab.local> We calibrate yearly our embedding center both the paraffin chambers and the cold plate, we record temp when used as a part of daily QC. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Tuesday, October 11, 2011 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cold plate temperatures on embedding station Hello All, We just got the Thermo Histostar. Curious to know if there is a CAP requirement for taking the cold plate temperatures on an embedding station? Need to know for quality control purposes. Thank you. Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Oct 11 10:55:53 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Oct 11 10:56:03 2011 Subject: [Histonet] RELIA Special Job Alert!! Exciting Opportunities from Coast to Coast 10-11-11 Message-ID: <1406F230C5F541C7ACB036DF37A418EF@ownerf1abaad51> Hi Histonetters!! Coast to Coast the histology field is blossoming again. If you were looking for a new opportunity but the timing wasn?t right or the location wasn?t right or the employers were moving waaaay toooo slooowww. Well things are different now!! I am very excited about the positions I am working on. I have placed people in positions with all of these employers in the past and they are a group of very happy histotechs. My clients offer excellent compensation, really nice benefits and great working environments. These are confirmed immediate full time permanent positions. If you are ASCP HT/HTL or eligible with 2-5 years of experience, my clients want to talk to you ASAP!!! If you are interested please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542 Here are the positions: MANAGEMENT Lab Manager ? Central Florida Histology Lab Manager - Modesto, CA Histology Manager ? Central Florida Histology Manager ? New York Histology Supervisor ? Portland, ME Histology Supervisor ? Portland, OR Assistant Histology Supervisor ? Austin, TX HISTOTECHNICIANS/HISTOTECHNOLOGISTS Histotechnician/Histotechnologist ? Long Island, NY Histology Tech ? Rochester, NY Histotech/PA ? Charlotte, NC Histotechnician/Histotechnologist ? Portland, OR Histotechnician ? Austin, TX IHC Tech ? Austin, TX Lead Grossing Tech ? Austin, TX Mohs Tech ? Fort Myers, FL Mohs Tech ? Lansing, MI Night Shift Histotech ? North Shore of Boston If you know someone who might be interested please feel free to pass the information along to them as well. I remember I offer a 500.00 referral fee if I place someone that you refer to me. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From awatanabe <@t> tgen.org Tue Oct 11 12:11:38 2011 From: awatanabe <@t> tgen.org (awatanabe@tgen.org) Date: Tue Oct 11 12:11:42 2011 Subject: [Histonet] Annexin V IHC staining in mouse xenograft Message-ID: Hi histonetters, I need some help. I am running an antibody from Biosensis called Annexin V in mouse xenograft brain samples with human glioma tumors treated and non-treated. I have incredible background that I can not get rid of any of my samples. I have used a protein block, rodent tissue block, post fixation, short antibody incubation time, short antigen retrieval time, very dilute antibody, polymer detection and every combination in between. I'm looking to see if anyone knows anything else to try. I'm trying to rearrange the protocol to put steps in different places to see if that works. At this point I'm not sure what else to try so any ideas would be great. Aprill Watanabe, B.S. Research Associate II Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Macroanalyte Analysis & Processing Center (MAPC) Translational Genomics Research Institute (TGen) 445 North 5th Street Phoenix, AZ 85004 Main: 602-343-8822 Fax: 602-343-8717 Cell: 602-481-8654 email: awatanabe@tgen.org website: www.tgen.org From jamie.erickson <@t> abbott.com Tue Oct 11 12:56:13 2011 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Tue Oct 11 12:56:22 2011 Subject: [Histonet] Microwave processor the Histo 5 for rodent tissue Message-ID: Hello histonetters, I'm wondering if anyone out there doing animal work has used the milestone histos 5 or other instrument to process rodent tissues. I would like your options on the quality. I would be interested to hear if this works well for IHC and bone decaling.. Thanks, Jamie Erickson Abbott Laboratories Worcester, MA 01605 508-688-3134 From af46 <@t> buffalo.edu Tue Oct 11 13:21:24 2011 From: af46 <@t> buffalo.edu (Featherstone, Annette) Date: Tue Oct 11 13:21:30 2011 Subject: [Histonet] staining for endothelial glycocalyx Message-ID: Does anyone have a staining protocol that will stain sugar structures on the surface of the endothelial cells?? Commonly structures are visable using SEM but I would like to know if it is possible to see it in a paraffin section. Annette From mcauliff <@t> umdnj.edu Tue Oct 11 13:27:10 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Oct 11 13:26:29 2011 Subject: [Histonet] staining for endothelial glycocalyx In-Reply-To: References: Message-ID: <4E948A7E.9000502@umdnj.edu> On 10/11/2011 2:21 PM, Featherstone, Annette wrote: > Does anyone have a staining protocol that will stain sugar structures on the surface of the endothelial cells?? Commonly structures are visable using SEM but I would like to know if it is possible to see it in a paraffin section. > > Annette > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Many years ago there were some papers on Lanthanum staining the surface coat of cells. While these were intended for TEM, I suspect one could use them for LM. I will see if I have some references. Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From histonet.nospam <@t> vneubert.com Tue Oct 11 13:36:20 2011 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Tue Oct 11 13:36:33 2011 Subject: [Histonet] Drying oven temps In-Reply-To: <9E67FDD215B226448638018A82B952BD026A16CCA401@EXCHANGE.slhn.org> References: <9E67FDD215B226448638018A82B952BD026A16CCA401@EXCHANGE.slhn.org> Message-ID: <4E948CA4.2050702@vneubert.com> 59 ?C, 1h, for all slides. > Is anyone willing to share their slide drying temperature and times? Does anyone know of a particular standard concerning this? > > Kimberly Artim, AST, HT (ASCP) > Technical Coordinator, Anatomic Pathology > St Lukes Hospital & Health Network > 801 Ostrum Street > Bethlehem, PA 18015 > 610-954-4832 > > > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Tue Oct 11 13:50:02 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Oct 11 13:50:09 2011 Subject: [Histonet] RE: staining for endothelial glycocalyx In-Reply-To: References: Message-ID: sounds like a job for Lectin Histochemistry Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Featherstone, Annette Sent: Tuesday, October 11, 2011 2:21 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] staining for endothelial glycocalyx Does anyone have a staining protocol that will stain sugar structures on the surface of the endothelial cells?? Commonly structures are visable using SEM but I would like to know if it is possible to see it in a paraffin section. Annette _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From kraniofacialbiology <@t> gmail.com Tue Oct 11 16:01:45 2011 From: kraniofacialbiology <@t> gmail.com (Kraniofacial Biology) Date: Tue Oct 11 16:01:51 2011 Subject: [Histonet] Regarding cartilage and bone staining Message-ID: Hello Histonetters I am new to this site. I am a clinician and very fresh to research. I was doing whole mount cartilage and bone staining of whole head of mice and was surprised to see whole mouse head being used for experiment. I was wondering if there is any other staining method which can be used in place of the whole mount cartilage and bone staining that would stain the sections on a slide and works on cartilage and bone. This would also save the whole mouse to be used and many slides can also be used for other experiments. Regards Pathik From liz <@t> premierlab.com Tue Oct 11 16:05:05 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Oct 11 16:06:34 2011 Subject: [Histonet] Regarding cartilage and bone staining In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA0BA9@SBS2K8.premierlab.local> You can always decal the sample and process to paraffin and stain for a wide variety of bone and cartilage stains. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kraniofacial Biology Sent: Tuesday, October 11, 2011 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Regarding cartilage and bone staining Hello Histonetters I am new to this site. I am a clinician and very fresh to research. I was doing whole mount cartilage and bone staining of whole head of mice and was surprised to see whole mouse head being used for experiment. I was wondering if there is any other staining method which can be used in place of the whole mount cartilage and bone staining that would stain the sections on a slide and works on cartilage and bone. This would also save the whole mouse to be used and many slides can also be used for other experiments. Regards Pathik _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Oct 11 16:19:52 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Oct 11 16:20:05 2011 Subject: [Histonet] FW: Open - - Histology Supervisor, UC San Francisco In-Reply-To: <8D7C2D242DBD45498006B21122072BF89448A249@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF89448A249@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <8D7C2D242DBD45498006B21122072BF89489D0CB@MCINFRWEM003.ucsfmedicalcenter.org> We have just opened the Histology / IHC Supervisor position at UC San Francisco. Apply online at: http://careers.ucsfmedicalcenter.org Search "Histology" to bring up postion. Posting reference number: 2651 Job Title: IPOX Lab Supervisor (HISTOTECHNOLOGIST, SUPVR) (Note, Supervisor of Histology AND Immunohistochemistry) Job Code:9068 Department: Pathology-Surgical / Histology Location: Parnassus Full/Part Time: Full-Time Regular/Temporary: Regular Shift: Not Applicable Weekly Hours: 40 Union Information: This classification is not represented by a union Appointment Type: Career At the University of California San Francisco Medical Center, teamwork and continual learning have maintained our top 10 ranking as one of "America's Best Hospitals" by U.S.News & World Report. For you, UCSF Medical Center is where you will share and discover something new every day with benefits and rewards that will last a lifetime. Job Summary: Under general direction from the Medical Director, the incumbent serves as supervisor to 3 Lead Histotechnologists and 9 Histotechnologists. In addition to supervisory duties, the incumbent serves as a technical expert providing direction to staff as well as performing all technical aspects of surgical histology and immunohistochemistry procedures. As supervisor: recruits, hires, trains, completes performance evaluations, and resolves employee issues. Provides orientation, completes competency assessments, maintains staff schedules, training and compliance documentation. Implements new tests and procedures as requested by Medical Directors. Updates and maintains the lab manuals and annual reviews required for accreditation. Integrates new technologies into the laboratory to include analyzing and selecting of new equipment that meets space constraints and operational needs. Ensures that new equipment and associated validation and staff training are performed. Ensures that all equipment is well-maintained. Ensures that staff training and documentation are completed as required. Ensures that QC documentation is complete and quality standards are maintained in the laboratory. Advises and assists researchers planning research projects and determines the ability of the laboratory to accommodate research projects, updating the Medical Director as required. Other duties as assigned. Required Qualifications: College degree in a biological science, chemistry or a related field or equivalent education and work experience experience as a histotechnologist in a comparable high-volume hospital histology laboratory within the last seven years, including senior-level experience. Demonstrated high-volume, high-quality sectioning and staining skills. Ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines. Excellent interpersonal and communication skills. ASCP certification: HT licensed or eligible required (candidate will be expected to obtain certification within 1 year), HTL desirable. Excellent interpersonal communication skills required. Demonstrated ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines required. Previous, recent supervisory experience in a Histology and /or Immunoperoxidase Laboratory required. Must be experienced with information technology systems and the use of hardware and software in business and laboratory settings. Must be an effective user of spreadsheets and database software. Preferred Qualifications: ASCP certification: HT or HTL-licensed strongly preferred. Previous leadership experience in a hospital laboratory. Required License/Certification: N/A From jessesnay <@t> hotmail.com Wed Oct 12 00:47:05 2011 From: jessesnay <@t> hotmail.com (Jessica Snay) Date: Wed Oct 12 00:47:10 2011 Subject: [Histonet] Cat Scratch fever Message-ID: Does anyone know where I can get a paraffin control block for cat scratch fever? From louise.renton <@t> gmail.com Wed Oct 12 01:50:10 2011 From: louise.renton <@t> gmail.com (Louise Renton) Date: Wed Oct 12 01:50:15 2011 Subject: [Histonet] Regarding cartilage and bone staining In-Reply-To: References: Message-ID: Hi Pathik, perhaps you could give us some background on what it is you are researching? On Tue, Oct 11, 2011 at 11:01 PM, Kraniofacial Biology < kraniofacialbiology@gmail.com> wrote: > Hello Histonetters > I am new to this site. I am a clinician and very fresh to research. I was > doing whole mount cartilage and bone staining of whole head of mice and was > surprised to see whole mouse head being used for experiment. I was > wondering > if there is any other staining method which can be used in place of the > whole mount cartilage and bone staining that would stain the sections on a > slide and works on cartilage and bone. This would also save the whole mouse > to be used and many slides can also be used for other experiments. > Regards > Pathik > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From Judith_Pardue <@t> memorial.org Wed Oct 12 06:11:38 2011 From: Judith_Pardue <@t> memorial.org (Pardue, Judith) Date: Wed Oct 12 06:11:53 2011 Subject: [Histonet] Methenamine Message-ID: <14B823F24E628E49BBFAD704E4BAB89A1A3222@chimsx03.CHI.catholichealth.net> I have tried to order methenamine and was told they no longer make it. We have tried many different companies that have it listed in their products but when you order it you are told it is no longer available. Does anyone know where we can get it or what is being used in its place. Judith Gale Pardue HT(ASCP), QIHC Histology Supervisor 423-495-5756 Memorial Healthcare System To the world I'm one, to one I'm the world This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From MAUGER <@t> email.chop.edu Wed Oct 12 06:35:37 2011 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Wed Oct 12 06:35:45 2011 Subject: [Histonet] Cat Scratch fever In-Reply-To: References: Message-ID: <443F5B475A9BF647AB962E834884EBAD27EFE68C98@EX7CCRPW03V1.chop.edu> Newcomer Supply sells cat scratch control slides. Jo -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Snay Sent: Wednesday, October 12, 2011 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cat Scratch fever Does anyone know where I can get a paraffin control block for cat scratch fever? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> cvmc.org Wed Oct 12 07:01:42 2011 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Wed Oct 12 07:01:51 2011 Subject: [Histonet] RE: Methenamine In-Reply-To: <14B823F24E628E49BBFAD704E4BAB89A1A3222@chimsx03.CHI.catholichealth.net> References: <14B823F24E628E49BBFAD704E4BAB89A1A3222@chimsx03.CHI.catholichealth.net> Message-ID: We get ours from Sigma-Aldrich (it is called Hexamethylenetetramine, ACS reagent) Catalog Number 398160-250G. We received it last May, so I assume that they still make it. Website is http://www.sigmaaldrich.com/united-states.html. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Wednesday, October 12, 2011 7:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Methenamine I have tried to order methenamine and was told they no longer make it. We have tried many different companies that have it listed in their products but when you order it you are told it is no longer available. Does anyone know where we can get it or what is being used in its place. Judith Gale Pardue HT(ASCP), QIHC Histology Supervisor 423-495-5756 Memorial Healthcare System To the world I'm one, to one I'm the world This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.byrnes <@t> accelpath.com Wed Oct 12 08:31:14 2011 From: a.byrnes <@t> accelpath.com (Andrew Byrnes) Date: Wed Oct 12 08:35:25 2011 Subject: [Histonet] Cat Scratch fever In-Reply-To: <443F5B475A9BF647AB962E834884EBAD27EFE68C98@EX7CCRPW03V1.chop.edu> References: <443F5B475A9BF647AB962E834884EBAD27EFE68C98@EX7CCRPW03V1.chop.edu> Message-ID: <6FD25FEA-71C0-4A59-A5B8-AA94C46AF586@accelpath.com> Good song! Andrew Byrnes AccelPath.com On Oct 12, 2011, at 7:35 AM, "Mauger, Joanne" wrote: > Newcomer Supply sells cat scratch control slides. > Jo > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Snay > Sent: Wednesday, October 12, 2011 1:47 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cat Scratch fever > > > Does anyone know where I can get a paraffin control block for cat scratch fever? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Wed Oct 12 08:37:45 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Oct 12 08:38:24 2011 Subject: [Histonet] Cat Scratch fever In-Reply-To: <6FD25FEA-71C0-4A59-A5B8-AA94C46AF586@accelpath.com> References: <443F5B475A9BF647AB962E834884EBAD27EFE68C98@EX7CCRPW03V1.chop.edu> <6FD25FEA-71C0-4A59-A5B8-AA94C46AF586@accelpath.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E17BC3B@evcspmbx3.ads.northwestern.edu> Too true! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Byrnes Sent: Wednesday, October 12, 2011 8:31 AM To: Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cat Scratch fever Good song! Andrew Byrnes AccelPath.com On Oct 12, 2011, at 7:35 AM, "Mauger, Joanne" wrote: > Newcomer Supply sells cat scratch control slides. > Jo > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Snay > Sent: Wednesday, October 12, 2011 1:47 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cat Scratch fever > > > Does anyone know where I can get a paraffin control block for cat scratch fever? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From T.Dettmering <@t> gsi.de Wed Oct 12 08:40:23 2011 From: T.Dettmering <@t> gsi.de (Dettmering, Till) Date: Wed Oct 12 08:40:30 2011 Subject: [Histonet] IHC-P compatible Serum amyloid P antibody against rat Message-ID: <090A6068BF577C42BA234FA37D48D70601006924@W2K3MAILSV.gsi.de> Hello everyone, I'm in need for an anti-rat-Serum amyloid P antibody which is IHC-P compatible. Suggestions, anyone? Thank you very much in advance! Best, Till GSI Helmholtzzentrum f?r Schwerionenforschung GmbH Planckstra?e 1 64291 Darmstadt www.gsi.de Gesellschaft mit beschr?nkter Haftung Sitz der Gesellschaft: Darmstadt Handelsregister: Amtsgericht Darmstadt, HRB 1528 Gesch?ftsf?hrung: Professor Dr. Dr. h.c. mult. Horst St?cker, Peter Hassenbach, Dr. Hartmut Eickhoff Vorsitzende des Aufsichtsrates: Dr. Beatrix Vierkorn-Rudolph Stellvertreter: Ministerialdirigent Dr. Rolf Bernhardt From joseph-galbraith <@t> uiowa.edu Wed Oct 12 09:19:40 2011 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Wed Oct 12 09:19:45 2011 Subject: [Histonet] Cat Scratch fever In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E17BC3B@evcspmbx3.ads.northwestern.edu> References: <443F5B475A9BF647AB962E834884EBAD27EFE68C98@EX7CCRPW03V1.chop.edu> <6FD25FEA-71C0-4A59-A5B8-AA94C46AF586@accelpath.com> <62C639732D3F274DACED033EBDF6ADAF1E17BC3B@evcspmbx3.ads.northwestern.edu> Message-ID: <6DC87DEA9229894DB3A09F8B61717A46106F8F34@hc-mailboxc1-n3.healthcare.uiowa.edu> No one under 30 would have a clue what that joke means. Lol Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, October 12, 2011 8:38 AM To: Andrew Byrnes; Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cat Scratch fever Too true! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Byrnes Sent: Wednesday, October 12, 2011 8:31 AM To: Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cat Scratch fever Good song! Andrew Byrnes AccelPath.com On Oct 12, 2011, at 7:35 AM, "Mauger, Joanne" wrote: > Newcomer Supply sells cat scratch control slides. > Jo > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Snay > Sent: Wednesday, October 12, 2011 1:47 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cat Scratch fever > > > Does anyone know where I can get a paraffin control block for cat scratch fever? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From victor <@t> pathology.washington.edu Wed Oct 12 09:28:54 2011 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Oct 12 09:28:58 2011 Subject: [Histonet] Cat Scratch fever In-Reply-To: <6DC87DEA9229894DB3A09F8B61717A46106F8F34@hc-mailboxc1-n3.healthcare.uiowa.edu> References: <443F5B475A9BF647AB962E834884EBAD27EFE68C98@EX7CCRPW03V1.chop.edu> <6FD25FEA-71C0-4A59-A5B8-AA94C46AF586@accelpath.com> <62C639732D3F274DACED033EBDF6ADAF1E17BC3B@evcspmbx3.ads.northwestern.edu> <6DC87DEA9229894DB3A09F8B61717A46106F8F34@hc-mailboxc1-n3.healthcare.uiowa.edu> Message-ID: <4E95A426.1030604@pathology.washington.edu> Google it and Ted Nugent is on the 1st page, although not the top spot. Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 10/12/2011 7:19 AM, Galbraith, Joe wrote: > No one under 30 would have a clue what that joke means. Lol Joe > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick > Sent: Wednesday, October 12, 2011 8:38 AM > To: Andrew Byrnes; Mauger, Joanne > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Cat Scratch fever > > Too true! > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Byrnes > Sent: Wednesday, October 12, 2011 8:31 AM > To: Mauger, Joanne > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Cat Scratch fever > > Good song! > > Andrew Byrnes > AccelPath.com > > On Oct 12, 2011, at 7:35 AM, "Mauger, Joanne" wrote: > >> Newcomer Supply sells cat scratch control slides. >> Jo >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Snay >> Sent: Wednesday, October 12, 2011 1:47 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Cat Scratch fever >> >> >> Does anyone know where I can get a paraffin control block for cat scratch fever? >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ________________________________ > Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. > ________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Wed Oct 12 09:42:38 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Oct 12 09:45:32 2011 Subject: [Histonet] Cat Scratch fever In-Reply-To: References: Message-ID: Try Ted Nugent, Inc. ...or you can get control slides from American Master Tech. http://www.americanmastertech.com/histology_control_slides.htm Brett -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Snay Sent: Wednesday, October 12, 2011 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cat Scratch fever Does anyone know where I can get a paraffin control block for cat scratch fever? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From akbitting <@t> geisinger.edu Wed Oct 12 09:49:44 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Oct 12 09:49:55 2011 Subject: [Histonet] Cat Scratch fever In-Reply-To: <6DC87DEA9229894DB3A09F8B61717A46106F8F34@hc-mailboxc1-n3.healthcare.uiowa.edu> References: <443F5B475A9BF647AB962E834884EBAD27EFE68C98@EX7CCRPW03V1.chop.edu> <6FD25FEA-71C0-4A59-A5B8-AA94C46AF586@accelpath.com> <62C639732D3F274DACED033EBDF6ADAF1E17BC3B@evcspmbx3.ads.northwestern.edu> <6DC87DEA9229894DB3A09F8B61717A46106F8F34@hc-mailboxc1-n3.healthcare.uiowa.edu> Message-ID: <4E9570C9.2B7F.00C9.1@geisinger.edu> I was just watching his show last night! That's entertainment! >>> "Galbraith, Joe" 10/12/2011 10:19 AM >>> No one under 30 would have a clue what that joke means. Lol Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, October 12, 2011 8:38 AM To: Andrew Byrnes; Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cat Scratch fever Too true! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Byrnes Sent: Wednesday, October 12, 2011 8:31 AM To: Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cat Scratch fever Good song! Andrew Byrnes AccelPath.com On Oct 12, 2011, at 7:35 AM, "Mauger, Joanne" wrote: > Newcomer Supply sells cat scratch control slides. > Jo > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Snay > Sent: Wednesday, October 12, 2011 1:47 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cat Scratch fever > > > Does anyone know where I can get a paraffin control block for cat scratch fever? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From AGleiberman <@t> cbiolabs.com Wed Oct 12 10:00:11 2011 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Wed Oct 12 10:00:20 2011 Subject: [Histonet] Bright M3500 microtome Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C0B58FEB5@cbiolabs05.CBiolabs.local> Hello everyone, Looking for opinion about Bright M3500 microtome - any advantages in comparison to Microm HM325 or Leica RM2235? Thanks Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From shive003 <@t> umn.edu Wed Oct 12 10:09:42 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Oct 12 10:09:57 2011 Subject: [Histonet] Annexin V IHC staining in mouse xenograft References: Message-ID: <2CE63082706849B5AB6B7A53B7CEF38F@auxs.umn.edu> Aprill, If you send us a list of the exact reagents and incubation times/dilutions that you use, someone on Histonet may be able to answer your question. Jan Shivers Univ. of Minnesota ----- Original Message ----- From: To: Sent: Tuesday, October 11, 2011 12:11 PM Subject: [Histonet] Annexin V IHC staining in mouse xenograft Hi histonetters, I need some help. I am running an antibody from Biosensis called Annexin V in mouse xenograft brain samples with human glioma tumors treated and non-treated. I have incredible background that I can not get rid of any of my samples. I have used a protein block, rodent tissue block, post fixation, short antibody incubation time, short antigen retrieval time, very dilute antibody, polymer detection and every combination in between. I'm looking to see if anyone knows anything else to try. I'm trying to rearrange the protocol to put steps in different places to see if that works. At this point I'm not sure what else to try so any ideas would be great. Aprill Watanabe, B.S. Research Associate II Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Macroanalyte Analysis & Processing Center (MAPC) Translational Genomics Research Institute (TGen) 445 North 5th Street Phoenix, AZ 85004 Main: 602-343-8822 Fax: 602-343-8717 Cell: 602-481-8654 email: awatanabe@tgen.org website: www.tgen.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Wed Oct 12 10:21:14 2011 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Oct 12 10:21:24 2011 Subject: [Histonet] Artisan barcode reading issue Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C367A6FF9@PEITHA.wad.pa-ucl.com> Good Morning Histonetters! Is anyone else having issues with reagent cartridges not reading on the Artisan staining system? We have had this instrument for quite some time and have started having problems with the barcodes not reading - a random cartridge from a kit - not always the same one - pretty much no rhyme or reason. The area of read has been cleaned (several times) but this continues to be an issue. Thanks in advance- Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From awatanabe <@t> tgen.org Wed Oct 12 11:06:29 2011 From: awatanabe <@t> tgen.org (awatanabe@tgen.org) Date: Wed Oct 12 11:06:35 2011 Subject: [Histonet] Annexin V IHC staining in mouse xenograft In-Reply-To: <2CE63082706849B5AB6B7A53B7CEF38F@auxs.umn.edu> Message-ID: Jan, Thank you for the input but I did get a lot of suggestions yesterday. Thank you all. Aprill Watanabe, B.S. Research Associate II Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Macroanalyte Analysis & Processing Center (MAPC) Translational Genomics Research Institute (TGen) 445 North 5th Street Phoenix, AZ 85004 Main: 602-343-8822 Fax: 602-343-8717 Cell: 602-481-8654 email: awatanabe@tgen.org website: www.tgen.org On 10/12/11 8:09 AM, "Jan Shivers" wrote: >Aprill, > >If you send us a list of the exact reagents and incubation >times/dilutions >that you use, someone on Histonet may be able to answer your question. > >Jan Shivers >Univ. of Minnesota > >----- Original Message ----- >From: >To: >Sent: Tuesday, October 11, 2011 12:11 PM >Subject: [Histonet] Annexin V IHC staining in mouse xenograft > > >Hi histonetters, >I need some help. I am running an antibody from Biosensis called Annexin >V >in mouse xenograft brain samples with human glioma tumors treated and >non-treated. I have incredible background that I can not get rid of any >of >my samples. I have used a protein block, rodent tissue block, post >fixation, short antibody incubation time, short antigen retrieval time, >very >dilute antibody, polymer detection and every combination in between. I'm >looking to see if anyone knows anything else to try. I'm trying to >rearrange the protocol to put steps in different places to see if that >works. At this point I'm not sure what else to try so any ideas would be >great. > >Aprill Watanabe, B.S. >Research Associate II >Integrated Cancer Genomics Division >Tissue Microarray Center (TMA) >Macroanalyte Analysis & Processing Center (MAPC) >Translational Genomics Research Institute (TGen) >445 North 5th Street >Phoenix, AZ 85004 >Main: 602-343-8822 >Fax: 602-343-8717 >Cell: 602-481-8654 >email: awatanabe@tgen.org >website: www.tgen.org > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lhotaks <@t> mcmaster.ca Wed Oct 12 10:46:36 2011 From: lhotaks <@t> mcmaster.ca (Sarka Lhotak) Date: Wed Oct 12 11:20:26 2011 Subject: [Histonet] Annexin V IHC in mouse xenograft Message-ID: Hi Aprill, I am not sure if you are using an antibody against Annexin V or if you are trying to stain with Annexin V (use it as a detection agent). Annexin V is a protein that binds to phosphatidyl serine. It is often used as a tool to detect early stages of apoptosis when the plasma membrane "flips" and exposes phosphatidyl serine on the outside of cells, whereas in normal live cells it would be on the inside. It is used eg. in flow cytometry on unfixed cells. Dead cells or permeabilized cells would be all positive as the reagent (Annexin V) would have access to the inside of cells and react with phosphatidyl serine in the inner leaflet of the membrane. I don't think it could work on tissues in paraffin sections. But somebody may prove me wrong... Sarka Lhotak McMaster University, Hamilton, Ontario From ruppert.amysue <@t> marshfieldclinic.org Wed Oct 12 11:47:19 2011 From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue) Date: Wed Oct 12 11:47:33 2011 Subject: [Histonet] Drying oven temps&In-Reply-To= Message-ID: <201110121647.p9CGlTA5028501@mailhost3.mfldclin.edu> For H&E slides, we use the Leica ST5020 autostainer. We have two ovens. The first oven is set for 40 C, 20 min. Then the slide rack is transferred to the second oven at 60 C, 15 min. WE have recently incorporated placing all of our slide racks for H&E in front of a small table top fan for 10-15 minutes prior to going onto the autostainer. This combination works well for us and does a great job of cutting down/ eliminating on the amount of nuclear bubbling artifact. We are not so stringent with our special stain slides. And IHC slides also have their own protocol. amysue ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From Clough <@t> medicine.tamhsc.edu Wed Oct 12 11:45:43 2011 From: Clough <@t> medicine.tamhsc.edu (Clough, Bret) Date: Wed Oct 12 11:48:46 2011 Subject: [Histonet] Processing and embedding mice lungs. Message-ID: Hello everyone! I have a student that wants to paraffin embed mice lungs, and I?ve only worked with bone tissue. I was wondering if someone in histo-land would be willing to share with me their protocol or their thoughts on how to proceed. The tissue will arrive inflated in a formalin fixative. Thank you, Bret Clough Texas A&M Health Science Center College of Medicine Temple, TX. From ruppert.amysue <@t> marshfieldclinic.org Wed Oct 12 11:49:28 2011 From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue) Date: Wed Oct 12 11:49:42 2011 Subject: [Histonet] Methenamine&In-Reply-To= Message-ID: <201110121649.p9CGnahb030370@mailhost3.mfldclin.edu> It is also available through Fisher. Hexamethylenetetramine H290-500 for a 500 gram bottle. amysue ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From cathy.crumpton <@t> tuality.org Wed Oct 12 12:24:48 2011 From: cathy.crumpton <@t> tuality.org (Cathy Crumpton) Date: Wed Oct 12 12:24:53 2011 Subject: [Histonet] "Population Served" compentency requirements Message-ID: I was wondering if anyone else was having to come up with a histology department competency with a focus on "population served". From what I understand it is a Joint Commission requirement for our hospital annual competencies. We are having a hard time coming up with something because we do not work with the public, just their specimens. Any ideas??? Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 From david.bonislawski <@t> umontana.edu Wed Oct 12 13:08:37 2011 From: david.bonislawski <@t> umontana.edu (David Bonislawski) Date: Wed Oct 12 13:08:43 2011 Subject: [Histonet] fixing live brain slices Message-ID: <1318442917.15369.YahooMailClassic@web161018.mail.bf1.yahoo.com> Does anyone know of a good method of fixing live brain slices that have been incubated in fluorescein diacetate? Upon drop-fixing the slices with 4%PFA I lose most to all fluorescent signal. Thank you for your time. Dave From HornHV <@t> archildrens.org Wed Oct 12 13:10:20 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Oct 12 13:10:25 2011 Subject: [Histonet] secure sette Message-ID: <25A4DE08332B19499904459F00AAACB719A93F38A1@EVS1.archildrens.org> Has anyone used the SecureSette Tissue Cassette? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Timothy.Morken <@t> ucsfmedctr.org Wed Oct 12 13:47:19 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Oct 12 13:47:28 2011 Subject: [Histonet] RE: secure sette In-Reply-To: <25A4DE08332B19499904459F00AAACB719A93F38A1@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719A93F38A1@EVS1.archildrens.org> Message-ID: <8D7C2D242DBD45498006B21122072BF89489D0D1@MCINFRWEM003.ucsfmedicalcenter.org> Hazel, we are trying them out right now. It looks really interesting and will save a LOT of time in embedding if it works out. We embedded 20 sample cassettes in less than 2 minutes yesterday. However, this cassette will not work for everything. For instance it is too short in length for most of our prostate bx. Some bx are too small for the integrated sponge to hold them in. Therefore the grossing staff has to decide on a case by case basis if the cassette is suitable. That brings up issues of how you load a cassette printer with a mix of regular and Securesette cassettes for optimum use. The grossers will need to be diligent as well in their tissue orientation because the cassette is not opened at embedding. I figure, however, that even is 30% of the cases can use this cassette it will save hours in the embedding area - we average 500 blocks/day. Tim Morken UCSF Pathology ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V [HornHV@archildrens.org] Sent: Wednesday, October 12, 2011 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] secure sette Has anyone used the SecureSette Tissue Cassette? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kraniofacialbiology <@t> gmail.com Wed Oct 12 14:11:36 2011 From: kraniofacialbiology <@t> gmail.com (Kraniofacial Biology) Date: Wed Oct 12 14:11:40 2011 Subject: [Histonet] Re: Regarding cartilage and bone staining In-Reply-To: References: Message-ID: Hello I would like to ask how many time do you put the mice head in xylene after series of Ethanol wash in the way of making paraffin block. I also did all the steps as well, but for embryonic day 17-18, even after observing clearing in xylene and moving to the several times in Gurr/paraplast I find the head to be soft and the initial part of head from which we start to cut is white and when I removed the flecks of wax and touched it was still soft. I am not getting what to do. Pathik On Tue, Oct 11, 2011 at 11:01 PM, Kraniofacial Biology < kraniofacialbiology@gmail.com> wrote: > Hello Histonetters > I am new to this site. I am a clinician and very fresh to research. I was > doing whole mount cartilage and bone staining of whole head of mice and was > surprised to see whole mouse head being used for experiment. I was wondering > if there is any other staining method which can be used in place of the > whole mount cartilage and bone staining that would stain the sections on a > slide and works on cartilage and bone. This would also save the whole mouse > to be used and many slides can also be used for other experiments. > Regards > Pathik > From liz <@t> premierlab.com Wed Oct 12 14:41:03 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Oct 12 14:42:31 2011 Subject: [Histonet] Re: Regarding cartilage and bone staining In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA0BD1@SBS2K8.premierlab.local> I'm not sure the size of the mouse head, but first of all the samples needs to be fixed properly I would let it stay in fixative for at least 48 hours. Then the sample would need to be decaled and that could take days depending upon the type of decal you choose to use. If you are processing the heads whole your processing cycle (depending upon the size of the samples) could range from 2 to 4 hours per station similar to something like this. I like to use three absolutes and 3 xylenes on bone samples and find that this works well. If the samples are over processed you can just cut back on the times. Liz 70% 2 hours 80% 2 hours 95% 2 hours 100% 2 hours 100% 2 hours 100% 2 hours Xylene 2 hours Xylene 2 hours Xylene 2 hours Paraffin 2 hours Paraffin 2 hours Paraffin 2 hours Paraffin 2 hours Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kraniofacial Biology Sent: Wednesday, October 12, 2011 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Regarding cartilage and bone staining Hello I would like to ask how many time do you put the mice head in xylene after series of Ethanol wash in the way of making paraffin block. I also did all the steps as well, but for embryonic day 17-18, even after observing clearing in xylene and moving to the several times in Gurr/paraplast I find the head to be soft and the initial part of head from which we start to cut is white and when I removed the flecks of wax and touched it was still soft. I am not getting what to do. Pathik On Tue, Oct 11, 2011 at 11:01 PM, Kraniofacial Biology < kraniofacialbiology@gmail.com> wrote: > Hello Histonetters > I am new to this site. I am a clinician and very fresh to research. I was > doing whole mount cartilage and bone staining of whole head of mice and was > surprised to see whole mouse head being used for experiment. I was wondering > if there is any other staining method which can be used in place of the > whole mount cartilage and bone staining that would stain the sections on a > slide and works on cartilage and bone. This would also save the whole mouse > to be used and many slides can also be used for other experiments. > Regards > Pathik > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed Oct 12 17:36:13 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Oct 12 17:36:18 2011 Subject: [Histonet] Annexin V IHC staining in mouse xenograft Message-ID: Hi, Have you considered running the antibody through some antibody purification beads. It isn't really a common histolgy thing to do, but the process isn't terribly difficult. It really sounds like a dirty antibody is at fault. It will probably result in a cleaner and more concentrated antibody. There are some commercially available kits to do this. I believe Invitrogen carries one and I'm almost sure Chemicon does too. Amos Message: 1 Date: Tue, 11 Oct 2011 17:11:38 +0000 From: Subject: [Histonet] Annexin V IHC staining in mouse xenograft To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi histonetters, I need some help. I am running an antibody from Biosensis called Annexin V in mouse xenograft brain samples with human glioma tumors treated and non-treated. I have incredible background that I can not get rid of any of my samples. I have used a protein block, rodent tissue block, post fixation, short antibody incubation time, short antigen retrieval time, very dilute antibody, polymer detection and every combination in between. I'm looking to see if anyone knows anything else to try. I'm trying to rearrange the protocol to put steps in different places to see if that works. At this point I'm not sure what else to try so any ideas would be great. Aprill Watanabe, B.S. Research Associate II From shrivastavaanuradha <@t> hotmail.com Wed Oct 12 19:21:50 2011 From: shrivastavaanuradha <@t> hotmail.com (anuradha shrivastava) Date: Wed Oct 12 19:21:55 2011 Subject: [Histonet] CAP COMMON CHECKLIST Message-ID: Hello every body, need your input for cap common checklist for PT(PROFICIENCY TESTING) evaluation in histology. we participate in Histoquip through CAP. want to know what other labs r doing? thanks a lot . anu. From NHeath <@t> Lifespan.org Thu Oct 13 05:34:54 2011 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Thu Oct 13 05:35:15 2011 Subject: [Histonet] different routes to obtain HT / HTL ASCP certification Message-ID: <130E8991F210424096EFC6F42EA33B240829C225@LSCOEXCH1.lsmaster.lifespan.org> Hi, I know this has been posted a bunch of times on here but can someone once again answer the question..."What are the different routes to obtaining an HT and an HTL. Thanks, Nancy Heath, HT (ASCP) From amber.mckenzie <@t> gastrodocs.net Thu Oct 13 08:24:02 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Oct 13 08:23:15 2011 Subject: [Histonet] RE: different routes to obtain HT / HTL ASCP certification In-Reply-To: <130E8991F210424096EFC6F42EA33B240829C225@LSCOEXCH1.lsmaster.lifespan.org> References: <130E8991F210424096EFC6F42EA33B240829C225@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: <5A33C952BB67F4468AF1F36D739212BC05F288@JERRY.Gia.com> Go to www.ascp.org and look it up -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heath, Nancy L. Sent: Thursday, October 13, 2011 5:35 AM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] different routes to obtain HT / HTL ASCP certification Hi, I know this has been posted a bunch of times on here but can someone once again answer the question..."What are the different routes to obtaining an HT and an HTL. Thanks, Nancy Heath, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Oct 13 09:11:26 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Oct 13 09:11:35 2011 Subject: [Histonet] Annexin V IHC staining in mouse xenograft In-Reply-To: References: Message-ID: <0E8850D1A44A4B21A34B266DAB031F77@prueggihctechlt> April, U might want to try FC receptor block, I get mine from Innovex. The folks at the NSH IHC Resouce Group may be able to help as well you can sign up with the group at www.ihcrg.org Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Wednesday, October 12, 2011 4:36 PM To: awatanabe@tgen.org; histonet@lists.utsouthwestern.edu Subject: [Histonet] Annexin V IHC staining in mouse xenograft Hi, Have you considered running the antibody through some antibody purification beads. It isn't really a common histolgy thing to do, but the process isn't terribly difficult. It really sounds like a dirty antibody is at fault. It will probably result in a cleaner and more concentrated antibody. There are some commercially available kits to do this. I believe Invitrogen carries one and I'm almost sure Chemicon does too. Amos Message: 1 Date: Tue, 11 Oct 2011 17:11:38 +0000 From: Subject: [Histonet] Annexin V IHC staining in mouse xenograft To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi histonetters, I need some help. I am running an antibody from Biosensis called Annexin V in mouse xenograft brain samples with human glioma tumors treated and non-treated. I have incredible background that I can not get rid of any of my samples. I have used a protein block, rodent tissue block, post fixation, short antibody incubation time, short antigen retrieval time, very dilute antibody, polymer detection and every combination in between. I'm looking to see if anyone knows anything else to try. I'm trying to rearrange the protocol to put steps in different places to see if that works. At this point I'm not sure what else to try so any ideas would be great. Aprill Watanabe, B.S. Research Associate II _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Oct 13 09:29:49 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Oct 13 09:29:54 2011 Subject: [Histonet] Thick cryosections In-Reply-To: <7922198191carmaga6@uv.es> References: <7922198191carmaga6@uv.es> Message-ID: <30A2F270724449CE8479B69A90361BF3@prueggihctechlt> I don't care what kink of slides you use if you cut 50 micron thick sections and try to do IHC you are going to lose some or all sections, in my experience. The only thing that might work and even this is questionable because of the thickness is the tape transfer system but I really doubt you are going to be able to get sections that thick to stick to the polymer coated slide and not come off with the tape. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carmen Maria Garcia Pascual Sent: Tuesday, October 11, 2011 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thick cryosections Good afternoon everyone! I have made 50um mice ovarian cryosections and I have used superfrost plus slides. But some of them fall of the slides when I perfomed the immuno, one colleague has told me that this is because the cuts are too thick, and I think she is rigth. I don't know if there is some thing that I can make to avoid this, like using another kind of slides... Thanks!!!!!!!!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Oct 13 09:33:49 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Oct 13 09:34:00 2011 Subject: [Histonet] RE: different routes to obtain HT / HTL ASCP certification In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC05F288@JERRY.Gia.com> Message-ID: Histotechnologist, HTL(ASCP) Application Fee: $210 To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; OR Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years. *laboratory accredited by a CMS approved accreditation organization (i.e., AABB, CAP, COLA, DNV, The Joint Commission, etc.) Clinical Laboratory Experience To fulfill the experience requirement for the Histotechnologist examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining Histotechnician, HT(ASCP) Application Fee: $185 To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Successful completion of a NAACLS accredited Histotechnician program within the last 5 years prior to the date of application for examination; OR Route 2: At least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, or an associate degree from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years. *Laboratory accredited by a CMS approved accreditation organization (i.e., AABB, CAP, COLA, DNV, The Joint Commission, etc.). Laboratory Experience To fulfill the experience requirement for the Histotechnician examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining Amber McKenzie Sent by: histonet-bounces@lists.utsouthwestern.edu 10/13/2011 06:25 AM To "Heath, Nancy L." , "histonet@lists.utsouthwestern.edu" cc "histonet-bounces@lists.utsouthwestern.edu" Subject [Histonet] RE: different routes to obtain HT / HTL ASCP certification Go to www.ascp.org and look it up -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heath, Nancy L. Sent: Thursday, October 13, 2011 5:35 AM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] different routes to obtain HT / HTL ASCP certification Hi, I know this has been posted a bunch of times on here but can someone once again answer the question..."What are the different routes to obtaining an HT and an HTL. Thanks, Nancy Heath, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha <@t> ka-recruiting.com Thu Oct 13 09:49:33 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Thu Oct 13 09:47:39 2011 Subject: [Histonet] Histology Job Opportunities Message-ID: <441754367.1318517372852.JavaMail.cfservice@sl4app2> Hi Histonet Members, I'm a healthcare recruiter and I help histology professionals find permanent jobs across the country. I'm working with quite a few hospitals/companies and I have listed my latest job opportunities. Please let me know if you have an interest in learning more about any of the locations or positions below! Histotech: AZ - Histotech GA - Histology Supervisor FL - Histotech 3rd shift IL - Histotech KY - Lead Histotech Maine - Histology Supervisor NC - Histotech 3rd shift NJ - Histotech 3rd shift NY - Westchester - Grosser NY - Long Island - Lab Manager of Anatomic Pathology NY - Rochester - Histotech PA - IHC Histotech TN - Histology Supervisor Cytotech: NY - NYC - Cytotechnologist Pathologist Assistant: NY - Long Island - Pathologist Assistant If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From Montina.VanMeter <@t> pbrc.edu Thu Oct 13 10:45:41 2011 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Thu Oct 13 10:45:53 2011 Subject: [Histonet] Thick cryosections In-Reply-To: <30A2F270724449CE8479B69A90361BF3@prueggihctechlt> References: <7922198191carmaga6@uv.es> <30A2F270724449CE8479B69A90361BF3@prueggihctechlt> Message-ID: Carmen, I routinely stain 40-60 micron sections for IHC. I currently run all my IHC with a free-floating protocol which allows for maximum staining penetration from both sides of the section and then mount them on the slides. In the past, we mounted the 60um sections on (300 bloom) gelatin coated slides that were processed by hand in a humidity chamber. If you would like additional information about this technique, please send me an email. Regards, Tina Montina J. Van Meter, HT (ASCP) Lab Manager Dept. of Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Thursday, October 13, 2011 9:30 AM To: 'Carmen Maria Garcia Pascual'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thick cryosections I don't care what kink of slides you use if you cut 50 micron thick sections and try to do IHC you are going to lose some or all sections, in my experience. The only thing that might work and even this is questionable because of the thickness is the tape transfer system but I really doubt you are going to be able to get sections that thick to stick to the polymer coated slide and not come off with the tape. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carmen Maria Garcia Pascual Sent: Tuesday, October 11, 2011 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thick cryosections Good afternoon everyone! I have made 50um mice ovarian cryosections and I have used superfrost plus slides. But some of them fall of the slides when I perfomed the immuno, one colleague has told me that this is because the cuts are too thick, and I think she is rigth. I don't know if there is some thing that I can make to avoid this, like using another kind of slides... Thanks!!!!!!!!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Oct 13 10:59:53 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Oct 13 10:59:59 2011 Subject: [Histonet] Thick cryosections In-Reply-To: References: <7922198191carmaga6@uv.es> <30A2F270724449CE8479B69A90361BF3@prueggihctechlt> Message-ID: <5BDD5E57DD3D4C3CA7ACE357CFC2A1C7@prueggihctechlt> Yes this was another method I was going to suggest, rather tedious isn't it? I have also had some success using elmers glue coated slides for things like this, I make them myself, 5% glue in dih20, dip the non charged slides in the glue then airdry, store them dry, then when you use them as they dip in water it seems to reactivate the glue and things stick, I have used this with success with GMA embedded cartilage and bone, cartilage especially tends to peel off the slide, there are so few cells and no vessels to attach to the slide with cartilage so it is a problem. It also helps to dry the slides flat on a slide warmer at about 40dc overnight, higher temps make the cartilage dislodge. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: Montina Van Meter [mailto:Montina.VanMeter@pbrc.edu] Sent: Thursday, October 13, 2011 9:46 AM To: Patsy Ruegg; Carmen Maria Garcia Pascual; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thick cryosections Carmen, I routinely stain 40-60 micron sections for IHC. I currently run all my IHC with a free-floating protocol which allows for maximum staining penetration from both sides of the section and then mount them on the slides. In the past, we mounted the 60um sections on (300 bloom) gelatin coated slides that were processed by hand in a humidity chamber. If you would like additional information about this technique, please send me an email. Regards, Tina Montina J. Van Meter, HT (ASCP) Lab Manager Dept. of Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Thursday, October 13, 2011 9:30 AM To: 'Carmen Maria Garcia Pascual'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thick cryosections I don't care what kink of slides you use if you cut 50 micron thick sections and try to do IHC you are going to lose some or all sections, in my experience. The only thing that might work and even this is questionable because of the thickness is the tape transfer system but I really doubt you are going to be able to get sections that thick to stick to the polymer coated slide and not come off with the tape. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carmen Maria Garcia Pascual Sent: Tuesday, October 11, 2011 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thick cryosections Good afternoon everyone! I have made 50um mice ovarian cryosections and I have used superfrost plus slides. But some of them fall of the slides when I perfomed the immuno, one colleague has told me that this is because the cuts are too thick, and I think she is rigth. I don't know if there is some thing that I can make to avoid this, like using another kind of slides... Thanks!!!!!!!!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Reuel.Cornelia <@t> tsrh.org Thu Oct 13 11:13:05 2011 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Thu Oct 13 11:13:29 2011 Subject: [Histonet] Substitute Xylene for Bone tissue Message-ID: <4E96C7C1.077E.00C5.1@tsrh.org> Does the Xylene substitute work very well with large bone tissue (5 to 7 cm long and between 3-5 mm thickness) for MMA and routine processing. What will be out pitfalls? We have a staff in our lab who is so "sensitive" to the smell of Xylene as she wanted to get pregnant sometime and the little smell that we have in our lab makes her emotional about it. Our lab has a very good ventilation but you could not prevent the smell when you open a retort after processing or even wiping small residue and placing it in our biohazard bin or simply cover slipping using xylene base mountant. I was planning to try the substitute to help make our environment a better place for everyone. Your helpful advise is always appreciated. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From joelleweaver <@t> hotmail.com Thu Oct 13 11:15:41 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Oct 13 11:15:46 2011 Subject: [Histonet] different routes to obtain HT / HTL ASCP certification In-Reply-To: <130E8991F210424096EFC6F42EA33B240829C225@LSCOEXCH1.lsmaster.lifespan.org> References: <130E8991F210424096EFC6F42EA33B240829C225@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: http://www.ascp.org/Board-of-Certification/GetCertified#tabs-1 They have a newly designed website, this is the link to the BOC/BOR registry routes and eligibility page. Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Thu, 13 Oct 2011 06:34:54 -0400 > From: NHeath@Lifespan.org > To: histonet@lists.utsouthwestern.edu > CC: histonet-bounces@lists.utsouthwestern.edu > Subject: [Histonet] different routes to obtain HT / HTL ASCP certification > > Hi, > > > > I know this has been posted a bunch of times on here but can someone > once again answer the question..."What are the different routes to > obtaining an HT and an HTL. > > > > Thanks, > > Nancy Heath, HT (ASCP) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Thu Oct 13 11:50:32 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Oct 13 11:50:36 2011 Subject: [Histonet] RE: Substitute Xylene for Bone tissue In-Reply-To: <4E96C7C1.077E.00C5.1@tsrh.org> References: <4E96C7C1.077E.00C5.1@tsrh.org> Message-ID: I personally do not use any xylenes substitute for any of my undemineralized bone/resin histology processing. However, I have heard mention that several people use the traditional xylenes substitutes in conjunction with at least one change of fresh xylenes and some even substitute xylenes with methyl salicylate (oil of wintergreen). In the past I would hear people complain that the xylenes substitutes did not do as good a job as regular xylenes, mostly due to inadequate removal of fat and especially in the larger animals with fatty marrow cavities, but maybe the xylenes substitutes have been optimized since then? Jack Date: Thu, 13 Oct 2011 11:13:05 -0500 From: Reuel.Cornelia@tsrh.org To: ratliffjack@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Substitute Xylene for Bone tissue Does the Xylene substitute work very well with large bone tissue (5 to 7 cm long and between 3-5 mm thickness) for MMA and routine processing. What will be out pitfalls? We have a staff in our lab who is so "sensitive" to the smell of Xylene as she wanted to get pregnant sometime and the little smell that we have in our lab makes her emotional about it. Our lab has a very good ventilation but you could not prevent the smell when you open a retort after processing or even wiping small residue and placing it in our biohazard bin or simply cover slipping using xylene base mountant. I was planning to try the substitute to help make our environment a better place for everyone. Your helpful advise is always appreciated. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 *************************************************************************** Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. *************************************************************************** From Barry.R.Rittman <@t> uth.tmc.edu Thu Oct 13 12:27:28 2011 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Oct 13 12:27:32 2011 Subject: [Histonet] Thick cryosections In-Reply-To: <30A2F270724449CE8479B69A90361BF3@prueggihctechlt> References: <7922198191carmaga6@uv.es> <30A2F270724449CE8479B69A90361BF3@prueggihctechlt> Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E901926A1472@UTHCMS1.uthouston.edu> Carmen One thing you might want to try is to treat these as free floating sections. While these are more difficult to handle it can be done. Have not done with ovaries (having worked in a dental school). Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Thursday, October 13, 2011 9:30 AM To: 'Carmen Maria Garcia Pascual'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thick cryosections I don't care what kink of slides you use if you cut 50 micron thick sections and try to do IHC you are going to lose some or all sections, in my experience. The only thing that might work and even this is questionable because of the thickness is the tape transfer system but I really doubt you are going to be able to get sections that thick to stick to the polymer coated slide and not come off with the tape. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carmen Maria Garcia Pascual Sent: Tuesday, October 11, 2011 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thick cryosections Good afternoon everyone! I have made 50um mice ovarian cryosections and I have used superfrost plus slides. But some of them fall of the slides when I perfomed the immuno, one colleague has told me that this is because the cuts are too thick, and I think she is rigth. I don't know if there is some thing that I can make to avoid this, like using another kind of slides... Thanks!!!!!!!!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Thu Oct 13 13:37:38 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Oct 13 13:38:16 2011 Subject: [Histonet] RE: Substitute Xylene for Bone tissue In-Reply-To: References: <4E96C7C1.077E.00C5.1@tsrh.org> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32096B66D1@Mail2Node2.ad.uams.edu> We used a xylene substitute (non-aliphatic Hydrocarbon not d-limonene) in with large animal bone and it was a great help. We always used the first clearing as a xylene for the defatting quality and assistance it gave to the overall specimen. It was explained to me by someone at the time that the xylene does the defatting and as we all know tends to harden bone to some extend while the substitute will (to an extent) allow some softening of the bone. This does not mean it gets too soft to use with MMA only just a better section in the end. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Thursday, October 13, 2011 11:51 AM To: Reuel Cornelia; Histonet Subject: [Histonet] RE: Substitute Xylene for Bone tissue I personally do not use any xylenes substitute for any of my undemineralized bone/resin histology processing. However, I have heard mention that several people use the traditional xylenes substitutes in conjunction with at least one change of fresh xylenes and some even substitute xylenes with methyl salicylate (oil of wintergreen). In the past I would hear people complain that the xylenes substitutes did not do as good a job as regular xylenes, mostly due to inadequate removal of fat and especially in the larger animals with fatty marrow cavities, but maybe the xylenes substitutes have been optimized since then? Jack Date: Thu, 13 Oct 2011 11:13:05 -0500 From: Reuel.Cornelia@tsrh.org To: ratliffjack@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Substitute Xylene for Bone tissue Does the Xylene substitute work very well with large bone tissue (5 to 7 cm long and between 3-5 mm thickness) for MMA and routine processing. What will be out pitfalls? We have a staff in our lab who is so "sensitive" to the smell of Xylene as she wanted to get pregnant sometime and the little smell that we have in our lab makes her emotional about it. Our lab has a very good ventilation but you could not prevent the smell when you open a retort after processing or even wiping small residue and placing it in our biohazard bin or simply cover slipping using xylene base mountant. I was planning to try the substitute to help make our environment a better place for everyone. Your helpful advise is always appreciated. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 *************************************************************************** Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. *************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From tissuearray <@t> hotmail.com Thu Oct 13 14:27:05 2011 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Thu Oct 13 14:27:08 2011 Subject: [Histonet] Best Paraffin For Tissue Microarray Cutting Message-ID: Hi All, We've been testing the best paraffin for constructing and cutting TMAs and we found that the Leica Paraplast Xtra was best for us. It cut nice and the sections stayed together well in the waterbath. We tried Richard Allen Type 9 but found it brittle and very hard to cut. Just wondering what paraffin other people are using? We use the Beecher Instrument for custom TMAs. And the Arraymold.com TMA Kits for constructing most everything else. Thanks for your time, Cheers, From NMargaryan <@t> childrensmemorial.org Thu Oct 13 15:20:48 2011 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Oct 13 15:23:41 2011 Subject: [Histonet] emergency question-IHC interruption Message-ID: Hi, Where I can stop my IHC, in what step? I started deparaffinization, but I need to stop and run my IHC tomorrow. Can I leave my slides in 95% Ethanol, or Water or I have to do HIER? Where to store slides: in refrigerator or room. Naira From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Oct 13 15:48:56 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Oct 13 15:49:36 2011 Subject: [Histonet] RE: emergency question-IHC interruption In-Reply-To: References: Message-ID: run them down to water or buffer and then leave them in buffer in the refrigerator. They should be fine. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira [NMargaryan@childrensmemorial.org] Sent: Thursday, October 13, 2011 4:20 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-request@lists.utsouthwestern.edu Subject: [Histonet] emergency question-IHC interruption Hi, Where I can stop my IHC, in what step? I started deparaffinization, but I need to stop and run my IHC tomorrow. Can I leave my slides in 95% Ethanol, or Water or I have to do HIER? Where to store slides: in refrigerator or room. Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu Oct 13 16:34:32 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Oct 13 16:34:36 2011 Subject: [Histonet] emergency question-IHC interruption In-Reply-To: References: Message-ID: I would hydrate to water and then store them in whatever buffer you use for your IHC, i.e. TRIS, PBS, etc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Thursday, October 13, 2011 3:21 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-request@lists.utsouthwestern.edu Subject: [Histonet] emergency question-IHC interruption Importance: High Hi, Where I can stop my IHC, in what step? I started deparaffinization, but I need to stop and run my IHC tomorrow. Can I leave my slides in 95% Ethanol, or Water or I have to do HIER? Where to store slides: in refrigerator or room. Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Thu Oct 13 16:39:41 2011 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Oct 13 16:42:28 2011 Subject: FW: [Histonet] emergency question-IHC interruption Message-ID: Thanks to all who are kindly and quick answered on my question. Naira On Thu, Oct 13, 2011 at 4:20 PM, Margaryan, Naira > wrote: Hi, Where I can stop my IHC, in what step? I started deparaffinization, but I need to stop and run my IHC tomorrow. Can I leave my slides in 95% Ethanol, or Water or I have to do HIER? Where to store slides: in refrigerator or room. Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lynn13361 <@t> aol.com Fri Oct 14 03:56:05 2011 From: lynn13361 <@t> aol.com (lynn13361@aol.com) Date: Fri Oct 14 03:56:23 2011 Subject: [Histonet] Fwd: Message-ID: <8CE58663DACCEE9-1EEC-20DA2@Webmail-m107.sysops.aol.com> http://hcauto.com/interesting.php?id=14 From lwhite38 <@t> cogeco.ca Fri Oct 14 06:18:28 2011 From: lwhite38 <@t> cogeco.ca (Lori White) Date: Fri Oct 14 06:18:33 2011 Subject: [Histonet] Tissue Processor In-Reply-To: <20111004164407.056B41B90@sunfep4.cogeco.net> References: <20111004164407.056B41B90@sunfep4.cogeco.net> Message-ID: <000001cc8a62$ffba9db0$ff2fd910$@ca> Hi Mike, We have both the VIP6 and TEC5 and are extremely happy with them. What you spend up front, you will save in repair costs, downtime and frustration. Lori ------------------------------ Message: 8 Date: Tue, 04 Oct 2011 11:56:03 -0400 From: "Mike Tighe" Subject: [Histonet] Tissue processor To: Message-ID: <4E8AF3E6.26E4.00EE.0@trudeauinstitute.org> Content-Type: text/plain; charset=US-ASCII I am looking to purchase a Processor and embedding center. I have received a quote for VIP6 and a TEC5 but they seem to be a bit out of our price range. Does anyone have another Processor/embedding center that they found to be reliable? Thanks Mike From sarahholt_ht <@t> yahoo.com Fri Oct 14 08:13:40 2011 From: sarahholt_ht <@t> yahoo.com (Sarah Holt) Date: Fri Oct 14 08:14:19 2011 Subject: [Histonet] Sox10 Message-ID: <118F1B7D-3176-4120-99B4-4E010165CE67@yahoo.com> Hello, I'm looking for a successful protocol for Sox10 on an automated platform, specifically the Bond. However, any protocol would be a great starting point. Dilutions, high/low ph and times as well as if you needed enhancer's to improve on signal. Thank you in advance for any suggestions!!! Sarah From kryan <@t> nfderm.com Fri Oct 14 11:36:19 2011 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Fri Oct 14 11:36:24 2011 Subject: [Histonet] pouring of liquid nitrogen into a cryostat Message-ID: Hi Everyone, Has anyone ever heard of pouring liquid nitrogen into the chamber of a cryostat that has warmed up to help cool it down more quickly? Does this do harm to the chamber or mechanisms of the cryostat? Any information would be appreciated. Thanks From leiker <@t> buffalo.edu Fri Oct 14 11:42:04 2011 From: leiker <@t> buffalo.edu (Leiker, Merced) Date: Fri Oct 14 11:42:14 2011 Subject: [Histonet] RE: pouring of liquid nitrogen into a cryostat In-Reply-To: References: Message-ID: I would definitely think it was cause components of the chamber as well as the chamber itself to crack. Liquid nitrogen is MUCH MUCH colder than any temperature your chamber would be, even at its coldest. The temp difference would be too extreme. Just my opinion having a bit of experience playing around with the stuff. Regards, Merced -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan Sent: Friday, October 14, 2011 12:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pouring of liquid nitrogen into a cryostat Hi Everyone, Has anyone ever heard of pouring liquid nitrogen into the chamber of a cryostat that has warmed up to help cool it down more quickly? Does this do harm to the chamber or mechanisms of the cryostat? Any information would be appreciated. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mandy.Bell <@t> chomp.org Fri Oct 14 11:43:50 2011 From: Mandy.Bell <@t> chomp.org (Bell, Mandy) Date: Fri Oct 14 11:45:49 2011 Subject: [Histonet] Biocare TB antibody on XT Message-ID: Hi, I was wondering if anyone is running the Biocare predilute Tuberculosis antibody on the Ventana XT and would be willing to share their protocol. We are having some trouble with background staining. Thanks, Mandy M Bell , HTL (ASCP) Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail From louise.renton <@t> gmail.com Fri Oct 14 11:59:40 2011 From: louise.renton <@t> gmail.com (Louise Renton) Date: Fri Oct 14 11:59:45 2011 Subject: [Histonet] pouring of liquid nitrogen into a cryostat In-Reply-To: References: Message-ID: bad idea - what about splash ups from the chamber? Very scary to contemplate On Fri, Oct 14, 2011 at 6:36 PM, Kaye Ryan wrote: > Hi Everyone, > > Has anyone ever heard of pouring liquid nitrogen into the chamber of a > cryostat that has warmed up to help cool it down more quickly? Does > this do harm to the chamber or mechanisms of the cryostat? Any > information would be appreciated. > > > > Thanks > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? From awatanabe <@t> tgen.org Fri Oct 14 12:07:24 2011 From: awatanabe <@t> tgen.org (awatanabe@tgen.org) Date: Fri Oct 14 12:07:30 2011 Subject: [Histonet] Re: Best Paraffin For Tissue Microarray Cutting In-Reply-To: <20111014170337.6D824D4C071@mr3.tgen.org> Message-ID: We use Blue Ribbon from Surgipath. I find that it handles the post arraying steps better and I like the contrast color while punching. It cuts nicely too. We use the manual arrayer from Estigen and have been for 8 years now. Aprill Watanabe, B.S. Research Associate II Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Macroanalyte Analysis & Processing Center (MAPC) Translational Genomics Research Institute (TGen) 445 North 5th Street Phoenix, AZ 85004 Main: 602-343-8822 Fax: 602-343-8717 Cell: 602-481-8654 email: awatanabe@tgen.org website: www.tgen.org On 10/14/11 10:03 AM, "histonet-request@lists.utsouthwestern.edu" wrote: >Best Paraffin For Tissue Microarray Cutting From rjbuesa <@t> yahoo.com Fri Oct 14 12:09:30 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 14 12:09:34 2011 Subject: [Histonet] pouring of liquid nitrogen into a cryostat In-Reply-To: Message-ID: <1318612170.77425.YahooMailClassic@web65713.mail.ac4.yahoo.com> Never heard of that and I would not do it at all. Just leave the cryostat to freeze itself. Ren? J. --- On Fri, 10/14/11, Kaye Ryan wrote: From: Kaye Ryan Subject: [Histonet] pouring of liquid nitrogen into a cryostat To: histonet@lists.utsouthwestern.edu Date: Friday, October 14, 2011, 12:36 PM Hi Everyone, Has anyone ever heard of pouring liquid nitrogen into the chamber of a cryostat that has warmed up to help cool it down more quickly?? Does this do harm to the chamber or mechanisms of the cryostat?? Any information would be appreciated. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kryan <@t> nfderm.com Fri Oct 14 13:00:47 2011 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Fri Oct 14 13:00:51 2011 Subject: [Histonet] pouring liquid nitrogen into the cryostat Message-ID: Thanks for all of the responses. We have been asked to do this and I agree with all of you! Bad idea! Have a great weekend. From SteveM <@t> mcclainlab.com Fri Oct 14 13:33:39 2011 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Fri Oct 14 13:20:33 2011 Subject: Histonet Digest, Vol 95, Issue 19 [Histonet] Tissue processor Message-ID: Mike, If your budget is tight, consider used Sakura VIP processor and Tissue-Tek embedding center. One superb vendor is Avantik , formerly Bellair in NJ. 800-783-9424 973-912-8900 They have earned my complete trust-I am reluctant to have equipment in my lab unless Bellair can maintain it. Every piece of equipment I have bought from them and serviced by them has been solid. A good used embedding center and processor can be serviceable for 10 years or more, yet inexpensive. New Tissue processor - Depending on your # of blocks, another consideration I did not see mentioned is the RVG2 tissue processor sold by Bellair-Avantik. We have used it since new with nearly 1100 runs over 3+ years without problem. One advantage is the preheater/heat exchanger pre-warming solutions, allowing for rapid processing, e.g., small biopsies in 90 minutes. Steve A. McClain, MD 631 361 4000 PS I have no financial interst in that company, but I have had great experience and I am biased toward small companies which deliver great service. Message: 8 Date: Tue, 04 Oct 2011 11:56:03 -0400 From: "Mike Tighe" Subject: [Histonet] Tissue processor To: Message-ID: <4E8AF3E6.26E4.00EE.0@trudeauinstitute.org> Content-Type: text/plain; charset=US-ASCII I am looking to purchase a Processor and embedding center. I have received a quote for VIP6 and a TEC5 but they seem to be a bit out of our price range. Does anyone have another Processor/embedding center that they found to be reliable? Thanks Mike From sauconym <@t> yahoo.com Sat Oct 15 03:47:08 2011 From: sauconym <@t> yahoo.com (Miha Tesar) Date: Sat Oct 15 03:47:13 2011 Subject: [Histonet] (no subject) Message-ID: <1318668428.32332.YahooMailNeo@web45007.mail.sp1.yahoo.com> Hi! I am working in Institute of Phatology Slovenia EU. Recently I have problems with muscle biopsy. There are a lots of artefact that I can not explain, like little holes in the tissue after the tissue is being frozen. We are using the isopentan and the liquid nitrogen for the freezing. Can enyone give me some ideas haw to avoid this artefact. How important is humidity in laboratory for the good results? The next problem is that after I put the tissue on the?microscope slides?after coloring the GTK all the tissue gets wrinkeld. Iam using the poly L lisin?microscope slides?or the Immuno microscope slides. Thx in advance! Best regards Miha from SLO From SteveM <@t> mcclainlab.com Sat Oct 15 07:40:57 2011 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Sat Oct 15 07:27:50 2011 Subject: [Histonet] RE: Histonet Digest, Vol 95, Issue 16 Drying Oven temps Message-ID: It is important to clarify the terminology-what you mean by 'drying' oven? The remarks below refer to the manual step of annealing unstained sections on slides prior to de-paraffinization and staining. The point or objection to the terminology of 'drying' oven relates to a far too common practice of wet slides on a slide warmer or in the 'drying' oven immediately which is visibly injurious to the tissue section, resulting in loss of contrast between H and E, leading to antigen loss and inconsistent IHC staining. In my opinion, this is an absolute NO. NO wet slides on a warmer or in a 'drying' oven unless you desire to prepare miniature protein 'soup' with purple staining rather than distinct red and blue staining. We stand slides upright along the slide warmer on a towel until visibly dry and shake-off the few remaining drops at the slide bottom edge before warming for a period, racking, and then proceeding to the annealing oven. Routinely for skin we anneal ('bake" is another commonly used yet imprecise term) racked previously dried slides for 20 min at 65-70 C for most stains. IHC slides can be annealed overnight at 60 C with good results. For a same day IHC case cut in the morning 60 minutes at 65-70 C for shaves and punch biopsies suffices, while 120 minutes improves excisions and large samples. (Note: These times may seem long, but it depends on the tissue and to some degree size of the sections, and the antigen retrieval method. Bear in mind for skin, preserving cornified layer, epidermis, dermal collagen, and SQ adipose tissue on the slide through heat antigen retrieval is a challenge.) For nails and hooves 80 C for 60 minutes suffices for PAS, PASD and GMS stains. Those with other special tissue experience, e.g., brain, skeletal muscle, bone, please speak up. I hope those with experience in more automated labs can speak to the temperatures in automated stainers and automated IHC having their own ovens. Finally, I hope someone with experience in in-situ PCR can speak to the needs of their procedure. Steve A. McClain, MD 631 361 4000 Message: 5 Date: Tue, 11 Oct 2011 20:36:20 +0200 From: "V. Neubert" Subject: Re: [Histonet] Drying oven temps To: histonet@lists.utsouthwestern.edu Message-ID: <4E948CA4.2050702@vneubert.com> Content-Type: text/plain; charset=ISO-8859-1 59 ?C, 1h, for all slides. > Is anyone willing to share their slide drying temperature and times? Does anyone know of a particular standard concerning this? > > Kimberly Artim, AST, HT (ASCP) > Technical Coordinator, Anatomic Pathology > St Lukes Hospital & Health Network > 801 Ostrum Street > Bethlehem, PA 18015 > 610-954-4832 From sadey <@t> hotmail.ca Sat Oct 15 18:55:37 2011 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Sat Oct 15 18:55:46 2011 Subject: [Histonet] medi tech users Message-ID: Hello: Can anyone tell me if they know how to assign QC's or Dr.s names to a batch of cases? We have to go into each case to change a Dr.s name. I'm also interested in assigning a QC number to a batch of cases with out having to go into each case. Thanks in advance. Sheila From sadey <@t> hotmail.ca Sat Oct 15 19:06:19 2011 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Sat Oct 15 19:06:24 2011 Subject: [Histonet] Looking for recommendations for a slide and cassette labeller system Message-ID: Hello:We are looking into purchasing a slide and cassette labeller system. Any advice would be great. Also, if you know which system to stay away from, that would be appreciated aswell.ThanksSheila From lmdee1 <@t> yahoo.com Sun Oct 16 13:12:55 2011 From: lmdee1 <@t> yahoo.com (Linda) Date: Sun Oct 16 13:13:00 2011 Subject: [Histonet] Looking for recommendations for a slide and cassette labeller system In-Reply-To: References: Message-ID: <1318788775.94848.YahooMailNeo@web36506.mail.mud.yahoo.com> Hi, ? I use the Brady system, which has two dimension bar coding.? They also have a scan to print, which has a modem for wireless use.? The customer service is outstanding.? The price is pretty reasonable too. ? ? Linda Dee, BGS, HT(ASCP) ? ________________________________ From: Sheila Adey To: histonet@lists.utsouthwestern.edu Sent: Saturday, October 15, 2011 7:06 PM Subject: [Histonet] Looking for recommendations for a slide and cassette labeller system Hello:We are looking into purchasing a slide and cassette labeller system. Any advice would be great. Also, if you know which system to stay away from, that would be appreciated aswell.ThanksSheila ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Sun Oct 16 17:34:09 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Oct 16 17:34:27 2011 Subject: [Histonet] (no subject) In-Reply-To: <1318668428.32332.YahooMailNeo@web45007.mail.sp1.yahoo.com> References: <1318668428.32332.YahooMailNeo@web45007.mail.sp1.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A7157609F4795@xmdb02.nch.kids> Miha, Are your muscles arising in saline? Receive them dry or in cell culture media (eg Hanks). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Miha Tesar Sent: Saturday, 15 October 2011 7:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi! I am working in Institute of Phatology Slovenia EU. Recently I have problems with muscle biopsy. There are a lots of artefact that I can not explain, like little holes in the tissue after the tissue is being frozen. We are using the isopentan and the liquid nitrogen for the freezing. Can enyone give me some ideas haw to avoid this artefact. How important is humidity in laboratory for the good results? The next problem is that after I put the tissue on the?microscope slides?after coloring the GTK all the tissue gets wrinkeld. Iam using the poly L lisin?microscope slides?or the Immuno microscope slides. Thx in advance! Best regards Miha from SLO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From crochieresteve <@t> aol.com Sun Oct 16 22:55:41 2011 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Sun Oct 16 17:54:44 2011 Subject: [Histonet] RE: Hi histone Message-ID: 67750d312163f4fa9e5501866fb8429e@[192.168.1.1] hey how have you been? Do you want to make some easy cash? http://t.co/SEgZGeUm You've got to read this story! I will never have to worry about the bills being paid on time again! From shrivastavaanuradha <@t> hotmail.com Sun Oct 16 18:33:41 2011 From: shrivastavaanuradha <@t> hotmail.com (anuradha shrivastava) Date: Sun Oct 16 18:33:48 2011 Subject: [Histonet] processing schedule for GI'S Message-ID: Hello Histo gurus, Could u please advice me on gi?s processing schedule for Leica asp 300. thanks in advance. From Tony_Reilly <@t> health.qld.gov.au Sun Oct 16 19:01:29 2011 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Sun Oct 16 19:01:53 2011 Subject: [Histonet] pouring of liquid nitrogen into a cryostat In-Reply-To: References: Message-ID: <4E9BFCF9.411C.0039.0@health.qld.gov.au> Hi Louise Not a very safe practice. I have heard of people using a bag of dry ice pellets rested against the knife holder to lower the temperature of the knife, both for when the chamber is too warm or the tissue needs to be cut at a lower temperature, such as fat. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> Louise Renton 10/15/2011 2:59 am >>> bad idea - what about splash ups from the chamber? Very scary to contemplate On Fri, Oct 14, 2011 at 6:36 PM, Kaye Ryan wrote: > Hi Everyone, > > Has anyone ever heard of pouring liquid nitrogen into the chamber of a > cryostat that has warmed up to help cool it down more quickly? Does > this do harm to the chamber or mechanisms of the cryostat? Any > information would be appreciated. > > > > Thanks > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From silas1 <@t> illinois.edu Sun Oct 16 22:47:48 2011 From: silas1 <@t> illinois.edu (Megan Silas) Date: Sun Oct 16 22:48:22 2011 Subject: [Histonet] Technovit 9100 New Message-ID: Hello, Has anyone used Technovit 9100 New? If so, what company did you order through - is there a distributor in the US? Also, have you found success with this in embedding undecalcified bone? Thanks for any and all advice! Megan Silas Megan Silas | megan.r.silas@gmail.com | 847-609-6336 University of Illinois at Urbana-Champaign | Bioengineering From ratliffjack <@t> hotmail.com Sun Oct 16 22:56:31 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Sun Oct 16 22:56:42 2011 Subject: [Histonet] Technovit 9100 New In-Reply-To: References: Message-ID: What is it you are trying to accomplish with staining? Can you tell us more about your project? I use Acrylosin SOFT Infiltration and Embedding solutions from Dorn and Hart Microedge (just up the road from you in Villa Park) for my thin 5 micron section and Acrylosin HARD for ground sections. Feel free to ask more questions as needed. Jack Senior Histologist, BioMimetic Therapeutics Chairman, Hard Tissue Committee - National Society for Histotechnology On Oct 16, 2011, at 10:47 PM, Megan Silas wrote: > Hello, > > Has anyone used Technovit 9100 New? If so, what company did you order > through - is there a distributor in the US? Also, have you found success > with this in embedding undecalcified bone? > > Thanks for any and all advice! > > Megan Silas > > Megan Silas | megan.r.silas@gmail.com | 847-609-6336 > University of Illinois at Urbana-Champaign | Bioengineering > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From SDattili <@t> stormontvail.org Mon Oct 17 07:49:07 2011 From: SDattili <@t> stormontvail.org (D'Attilio, Shelley) Date: Mon Oct 17 07:49:15 2011 Subject: [Histonet] RE: Recommendations for a slide and cassette labeler system In-Reply-To: Message-ID: Hi all, Sheila asked for recommendations for a slide and cassette labeler system. We use the Leica IPS and IPC printers and we are very happy with their reliability and performance. I believe they are larger than other printers but well worth the real estate, in my opinion. Our printers are interfaced to our A/P LIS (WinSurge), but we also have the ability to print slides and cassettes as needed through the printing software. Be advised that our installation required that each printer be connected to its own PC and I needed to purchase two interfaces from WinSurge, so it wasn't an inexpensive project by any means. I think the Leica labelers are suitable for a high-volume laboratory, but may be overkill for a lab with smaller volumes. Regards, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Oct 17 08:17:33 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Oct 17 08:17:39 2011 Subject: [Histonet] HHV-6 Antibody Message-ID: Hi Histonet. Can anyone recommend a HHV-6 antibody to use in formalin fixed paraffin embedded tissues. Preferably ASR or IVD. But I'll take whatever suggestions. Thanks in advance. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From BDeBrosse-Serra <@t> isisph.com Mon Oct 17 09:12:45 2011 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Mon Oct 17 09:13:12 2011 Subject: [Histonet] (no subject) In-Reply-To: <1318668428.32332.YahooMailNeo@web45007.mail.sp1.yahoo.com> References: <1318668428.32332.YahooMailNeo@web45007.mail.sp1.yahoo.com> Message-ID: <493CAA64F203E14E8823737B9EE0E25F091E912710@EXCHMB01.isis.local> Do you coat the muscle biopsies with talcum powder prior to freezing? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Miha Tesar Sent: Saturday, October 15, 2011 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi! I am working in Institute of Phatology Slovenia EU. Recently I have problems with muscle biopsy. There are a lots of artefact that I can not explain, like little holes in the tissue after the tissue is being frozen. We are using the isopentan and the liquid nitrogen for the freezing. Can enyone give me some ideas haw to avoid this artefact. How important is humidity in laboratory for the good results? The next problem is that after I put the tissue on the?microscope slides?after coloring the GTK all the tissue gets wrinkeld. Iam using the poly L lisin?microscope slides?or the Immuno microscope slides. Thx in advance! Best regards Miha from SLO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Mon Oct 17 12:52:37 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Mon Oct 17 12:52:42 2011 Subject: [Histonet] Attendees of the SCSHT Fall Meeting Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27311CDDAE@NADCWPMSGCMS03.hca.corpad.net> The group rate for the hotel rooms has been extended until Friday October 21, 2011. After that date you can book a room and get the group rate based on availability at time of booking. Please get your registrations sent to : Vann Roberts 206 Rhodehaven Drive Anderson, SC 29625 Anyone needing a program, I can send one via email. Thank, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From Margaret.Perry <@t> sdstate.edu Mon Oct 17 13:27:48 2011 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Oct 17 13:43:01 2011 Subject: [Histonet] Need help with Laminin Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE01CAFC@SDSU-EX03.jacks.local> I am trying to work up Laminin in dogs and cats. I have tried the antibody from Sigma L9393 with a variety of retrieval methods both enzymatic and HEIR with no success. I would like to hear from anyone who is testing for laminin. Do I need to use a different antibody? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From liz <@t> premierlab.com Mon Oct 17 13:54:42 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Oct 17 13:56:12 2011 Subject: [Histonet] RE: Need help with Laminin In-Reply-To: <25F4FBA34BE9D142964ECC4525B82AEE01CAFC@SDSU-EX03.jacks.local> Message-ID: <14E2C6176416974295479C64A11CB9AE1DECBA0C14@SBS2K8.premierlab.local> We use the laminin from abcam ab11575, have not tried on canine or cat but it works in bovine and porcine. We have also tried the one from Dako but I'm not sure of the species cross reactivity with that one. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Perry, Margaret Sent: Monday, October 17, 2011 12:28 PM To: ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: [IHCRG] Need help with Laminin I am trying to work up Laminin in dogs and cats. I have tried the antibody from Sigma L9393 with a variety of retrieval methods both enzymatic and HEIR with no success. I would like to hear from anyone who is testing for laminin. Do I need to use a different antibody? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. From talulahgosh <@t> gmail.com Mon Oct 17 14:36:55 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Oct 17 14:36:58 2011 Subject: [Histonet] pouring of liquid nitrogen into a cryostat In-Reply-To: References: Message-ID: I would suggest dry ice (on top of something plastic on top of the metal shelves), NOT liquid nitrogen. Dry ice is usually cold enough to bring our cryostat to -28 C when it won't cool down past -24 C because of frost on the compressor. I don't think it actually makes the cryostat colder than that. Also, you don't really need that much, just a medium sized chunk. Be careful not to get your face too close to the chamber because remember, it is evaporating carbon dioxide!! Worse comes to worse, I guess you could use plain ice, but that would probably melt faster than you could use it. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Oct 14, 2011 at 12:36 PM, Kaye Ryan wrote: > Hi Everyone, > > Has anyone ever heard of pouring liquid nitrogen into the chamber of a > cryostat that has warmed up to help cool it down more quickly? Does > this do harm to the chamber or mechanisms of the cryostat? Any > information would be appreciated. > > > > Thanks > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Rcartun <@t> harthosp.org Mon Oct 17 14:39:01 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Oct 17 14:39:10 2011 Subject: [Histonet] Question - Prostate biopsy reports Message-ID: <4E9C4C15.7400.0077.1@harthosp.org> How many of you who are reading prostate biopsy specimens in a hospital laboratory incorporate a diagram of the prostate in your report that shows the anatomical location of "benign, PIN, and adenocarcinoma" pathology in each quadrant? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From becky.garrison <@t> jax.ufl.edu Mon Oct 17 15:40:46 2011 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Mon Oct 17 15:40:52 2011 Subject: [Histonet] (no subject) In-Reply-To: <1318668428.32332.YahooMailNeo@web45007.mail.sp1.yahoo.com> References: <1318668428.32332.YahooMailNeo@web45007.mail.sp1.yahoo.com> Message-ID: <9E47DE9D490DCC42A2EAE94F22BF93F2094D1C13@JX1B-MAIL1.umc.ufl.edu> I am forwarding this response from a fellow worker, D Kaylor (The techs here have consistently good results when freezing fresh muscles for enzyme histochemistry. The small holes are freeze artifact. Usually they occur when the muscle is not frozen fast enough. We use methyl butane floating in a liquid nitrogen bath. Either way, the temp is critical. It needs to be at least -155 degrees C. The muscle must be submerged and held under for about 45 seconds to one minute. Do not start or dip the muscle. Once started it must be held submerged. We have never had any issues with humidity. Are the muscle bx swimming in saline? We have better results when they are submitted on saline moistened gauze. We use positively charged slides to pick up our sections. We have some wrinkles but that is due to the sectioning of frozen tissue not the type of slide. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Miha Tesar Sent: Saturday, October 15, 2011 4:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi! I am working in Institute of Phatology Slovenia EU. Recently I have problems with muscle biopsy. There are a lots of artefact that I can not explain, like little holes in the tissue after the tissue is being frozen. We are using the isopentan and the liquid nitrogen for the freezing. Can enyone give me some ideas haw to avoid this artefact. How important is humidity in laboratory for the good results? The next problem is that after I put the tissue on the?microscope slides?after coloring the GTK all the tissue gets wrinkeld. Iam using the poly L lisin?microscope slides?or the Immuno microscope slides. Thx in advance! Best regards Miha from SLO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sally.norton <@t> seattlechildrens.org Mon Oct 17 16:36:24 2011 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Mon Oct 17 16:36:36 2011 Subject: [Histonet] (no subject) In-Reply-To: <9E47DE9D490DCC42A2EAE94F22BF93F2094D1C13@JX1B-MAIL1.umc.ufl.edu> References: <1318668428.32332.YahooMailNeo@web45007.mail.sp1.yahoo.com> <9E47DE9D490DCC42A2EAE94F22BF93F2094D1C13@JX1B-MAIL1.umc.ufl.edu> Message-ID: <1357F84B33D39A46BA015A8EC6ABCBD00219C7@PPWEXD01a.childrens.sea.kids> Hi Miha, we do many muscle bxs here as well, & the information Becky has sent you is exactly how we do it. This should help you! Sally Norton Seattle Children's Hospital Seattle, WA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garrison, Becky Sent: Monday, October 17, 2011 1:41 PM To: 'Miha Tesar'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) I am forwarding this response from a fellow worker, D Kaylor (The techs here have consistently good results when freezing fresh muscles for enzyme histochemistry. The small holes are freeze artifact. Usually they occur when the muscle is not frozen fast enough. We use methyl butane floating in a liquid nitrogen bath. Either way, the temp is critical. It needs to be at least -155 degrees C. The muscle must be submerged and held under for about 45 seconds to one minute. Do not start or dip the muscle. Once started it must be held submerged. We have never had any issues with humidity. Are the muscle bx swimming in saline? We have better results when they are submitted on saline moistened gauze. We use positively charged slides to pick up our sections. We have some wrinkles but that is due to the sectioning of frozen tissue not the type of slide. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Miha Tesar Sent: Saturday, October 15, 2011 4:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi! I am working in Institute of Phatology Slovenia EU. Recently I have problems with muscle biopsy. There are a lots of artefact that I can not explain, like little holes in the tissue after the tissue is being frozen. We are using the isopentan and the liquid nitrogen for the freezing. Can enyone give me some ideas haw to avoid this artefact. How important is humidity in laboratory for the good results? The next problem is that after I put the tissue on the?microscope slides?after coloring the GTK all the tissue gets wrinkeld. Iam using the poly L lisin?microscope slides?or the Immuno microscope slides. Thx in advance! Best regards Miha from SLO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From ruebenjcarter <@t> gmail.com Mon Oct 17 18:19:13 2011 From: ruebenjcarter <@t> gmail.com (R C) Date: Mon Oct 17 18:19:17 2011 Subject: [Histonet] Re: Looking for recommendations for a slide and cassette labeller Message-ID: Hi Sheila. As a recent user of the Leica systems for cassette and slide labelling (IP C and S), I recommend them both. The single user console can control both units and although a PC is required you won't be dissatisfied. Stream lining and efficiency seem to suit the units well. Multiple lines of metadata can be written across the blocks as well as the slides. You should look into the units to see if they'd be a fit for your laboratory. On Sun, Oct 16, 2011 at 10:01 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. medi tech users (Sheila Adey) > 2. Looking for recommendations for a slide and cassette labeller > system (Sheila Adey) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 15 Oct 2011 19:55:37 -0400 > From: Sheila Adey > Subject: [Histonet] medi tech users > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Hello: > Can anyone tell me if they know how to assign QC's or Dr.s names to a > batch of cases? We have to go into each case to change a Dr.s name. > I'm also interested in assigning a QC number to a batch of cases with out > having to go into each case. > Thanks in advance. > Sheila > > ------------------------------ > > Message: 2 > Date: Sat, 15 Oct 2011 20:06:19 -0400 > From: Sheila Adey > Subject: [Histonet] Looking for recommendations for a slide and > cassette labeller system > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Hello:We are looking into purchasing a slide and cassette labeller system. > Any advice would be great. Also, if you know which system to stay away from, > that would be appreciated aswell.ThanksSheila > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 95, Issue 21 > **************************************** > From ruebenjcarter <@t> gmail.com Mon Oct 17 18:26:04 2011 From: ruebenjcarter <@t> gmail.com (R C) Date: Mon Oct 17 18:26:09 2011 Subject: [Histonet] Re: (no subject) Miha from SLO Message-ID: Hi Miha. Sounds like pre-freezing artifact. Make sure that the isopentane and pre-cooled adequately and the specimens are frozen quickly. On Mon, Oct 17, 2011 at 10:01 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Looking for recommendations for a slide and cassette > labeller system (Linda) > 2. RE: (no subject) (Tony Henwood) > 3. RE: Hi histone (crochieresteve@aol.com) > 4. processing schedule for GI'S (anuradha shrivastava) > 5. Re: pouring of liquid nitrogen into a cryostat (Tony Reilly) > 6. Technovit 9100 New (Megan Silas) > 7. Re: Technovit 9100 New (Jack Ratliff) > 8. RE: Recommendations for a slide and cassette labeler system > (D'Attilio, Shelley) > 9. HHV-6 Antibody (McMahon, Loralee A) > 10. RE: (no subject) (Bea DeBrosse-Serra) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 16 Oct 2011 11:12:55 -0700 (PDT) > From: Linda > Subject: Re: [Histonet] Looking for recommendations for a slide and > cassette labeller system > To: Sheila Adey , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1318788775.94848.YahooMailNeo@web36506.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi, > > I use the Brady system, which has two dimension bar coding. They also have > a scan to print, which has a modem for wireless use. The customer service > is outstanding. The price is pretty reasonable too. > > > Linda Dee, BGS, HT(ASCP) > > > > ________________________________ > From: Sheila Adey > To: histonet@lists.utsouthwestern.edu > Sent: Saturday, October 15, 2011 7:06 PM > Subject: [Histonet] Looking for recommendations for a slide and cassette > labeller system > > > Hello:We are looking into purchasing a slide and cassette labeller system. > Any advice would be great. Also, if you know which system to stay away from, > that would be appreciated aswell.ThanksSheila > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 2 > Date: Sun, 16 Oct 2011 22:34:09 +0000 > From: Tony Henwood > Subject: RE: [Histonet] (no subject) > To: "'Miha Tesar'" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: <6D6BD1DE8A5571489398B392A38A7157609F4795@xmdb02.nch.kids> > Content-Type: text/plain; charset="iso-8859-1" > > Miha, > > Are your muscles arising in saline? > Receive them dry or in cell culture media (eg Hanks). > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Miha Tesar > Sent: Saturday, 15 October 2011 7:47 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > Hi! > I am working in Institute of Phatology Slovenia EU. Recently I have > problems with muscle biopsy. There are a lots of artefact that I can not > explain, like little holes in the tissue after the tissue is being frozen. > We are using the isopentan and the liquid nitrogen for the freezing. Can > enyone give me some ideas haw to avoid this artefact. How important is > humidity in laboratory for the good results? > The next problem is that after I put the tissue on the microscope > slides after coloring the GTK all the tissue gets wrinkeld. Iam using the > poly L lisin microscope slides or the Immuno microscope slides. > Thx in advance! > Best regards Miha from SLO > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, please delete it and notify the > sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned and > although no computer viruses were detected, The Childrens Hospital at > Westmead accepts no liability for any consequential damage resulting from > email containing computer viruses. > > ********************************************************************************* > > > > ------------------------------ > > Message: 3 > Date: Sun, 16 Oct 2011 22:55:41 > From: crochieresteve@aol.com > Subject: [Histonet] RE: Hi histone > To: histonet@lists.utsouthwestern.edu > Message-ID: 67750d312163f4fa9e5501866fb8429e@[192.168.1.1] > Content-Type: text/plain; charset="iso-8859-1" > > hey how have you been? Do you want to make some easy cash? > http://t.co/SEgZGeUm You've got to read this story! I will never have to > worry about the bills being paid on time again! > > > > ------------------------------ > > Message: 4 > Date: Sun, 16 Oct 2011 19:33:41 -0400 > From: anuradha shrivastava > Subject: [Histonet] processing schedule for GI'S > To: > Message-ID: > Content-Type: text/plain; charset="utf-8" > > Hello Histo gurus, > Could u please advice me on gi???s processing schedule for Leica asp 300. > thanks in advance. > > > ------------------------------ > > Message: 5 > Date: Mon, 17 Oct 2011 10:01:29 +1000 > From: "Tony Reilly" > Subject: Re: [Histonet] pouring of liquid nitrogen into a cryostat > To: "Louise Renton" , "Histonet" > > Message-ID: <4E9BFCF9.411C.0039.0@health.qld.gov.au> > Content-Type: text/plain; charset="us-ascii" > > Hi Louise > > Not a very safe practice. I have heard of people using a bag of dry ice > pellets rested against the knife holder to lower the temperature of the > knife, both for when the chamber is too warm or the tissue needs to be cut > at a lower temperature, such as fat. > > regards > Tony > > > > > Tony Reilly B.App.Sc. , M.Sc. > Chief Scientist, Anatomical Pathology > Pathology Queensland-PA Laboratory > _________________________________________________ > Clinical and Statewide Services Division| QueenslandHealth > > Level 1, Building 15,Princess Alexandra Hospital > Ipswich Road,WOOLLOONGABBA Qld4102 > Ph: 07 3176 2412 > Mob: 0402 139411 > Fax: 07 3176 2930 > Email: tony_reilly@health.qld.gov.au > Web: www.health.qld.gov.au/qhcss/ > > > > > >>> Louise Renton 10/15/2011 2:59 am >>> > bad idea - what about splash ups from the chamber? Very scary to > contemplate > > On Fri, Oct 14, 2011 at 6:36 PM, Kaye Ryan wrote: > > > Hi Everyone, > > > > Has anyone ever heard of pouring liquid nitrogen into the chamber of a > > cryostat that has warmed up to help cool it down more quickly? Does > > this do harm to the chamber or mechanisms of the cryostat? Any > > information would be appreciated. > > > > > > > > Thanks > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > +27 11 717 2298 (tel & fax) > 073 5574456 (emergencies only) > Question: Are rhinos overweight unicorns? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ******************************************************************************** > This email, including any attachments sent with it, is confidential and for > the sole use of the intended recipient(s). This confidentiality is not > waived or lost, if you receive it and you are not the intended recipient(s), > or if it is transmitted/received in error. > Any unauthorised use, alteration, disclosure, distribution or review of > this email is strictly prohibited. The information contained in this email, > including any attachment sent with it, may be subject to a statutory duty of > confidentiality if it relates to health service matters. > If you are not the intended recipient(s), or if you have received this > email in error, you are asked to immediately notify the sender by telephone > collect on Australia +61 1800 198 175 or by return email. You should also > delete this email, and any copies, from your computer system network and > destroy any hard copies produced. > If not an intended recipient of this email, you must not copy, distribute > or take any action(s) that relies on it; any form of disclosure, > modification, distribution and/or publication of this email is also > prohibited. > Although Queensland Health takes all reasonable steps to ensure this email > does not contain malicious software, Queensland Health does not accept > responsibility for the consequences if any person's computer inadvertently > suffers any disruption to services, loss of information, harm or is infected > with a virus, other malicious computer programme or code that may occur as a > consequence of receiving this email. > Unless stated otherwise, this email represents only the views of the sender > and not the views of the Queensland Government. > > ********************************************************************************** > > > > ------------------------------ > > Message: 6 > Date: Sun, 16 Oct 2011 22:47:48 -0500 > From: Megan Silas > Subject: [Histonet] Technovit 9100 New > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=ISO-8859-1 > > Hello, > > Has anyone used Technovit 9100 New? If so, what company did you order > through - is there a distributor in the US? Also, have you found success > with this in embedding undecalcified bone? > > Thanks for any and all advice! > > Megan Silas > > Megan Silas | megan.r.silas@gmail.com | 847-609-6336 > University of Illinois at Urbana-Champaign | Bioengineering > > > ------------------------------ > > Message: 7 > Date: Sun, 16 Oct 2011 22:56:31 -0500 > From: Jack Ratliff > Subject: Re: [Histonet] Technovit 9100 New > To: Megan Silas > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > What is it you are trying to accomplish with staining? Can you tell us more > about your project? I use Acrylosin SOFT Infiltration and Embedding > solutions from Dorn and Hart Microedge (just up the road from you in Villa > Park) for my thin 5 micron section and Acrylosin HARD for ground sections. > Feel free to ask more questions as needed. > > > Jack > > > Senior Histologist, BioMimetic Therapeutics > Chairman, Hard Tissue Committee - National Society for Histotechnology > > > > On Oct 16, 2011, at 10:47 PM, Megan Silas wrote: > > > Hello, > > > > Has anyone used Technovit 9100 New? If so, what company did you order > > through - is there a distributor in the US? Also, have you found success > > with this in embedding undecalcified bone? > > > > Thanks for any and all advice! > > > > Megan Silas > > > > Megan Silas | megan.r.silas@gmail.com | 847-609-6336 > > University of Illinois at Urbana-Champaign | Bioengineering > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 8 > Date: Mon, 17 Oct 2011 07:49:07 -0500 > From: "D'Attilio, Shelley" > Subject: [Histonet] RE: Recommendations for a slide and cassette > labeler system > To: > Message-ID: > < > B7244AC045486C4190DE73F226F432D4221FBD02@EXCHANGE-VS1.stormontvail.org> > > Content-Type: text/plain; charset="iso-8859-1" > > Hi all, > Sheila asked for recommendations for a slide and cassette labeler system. > We use the Leica IPS and IPC printers and we are very happy with their > reliability and performance. I believe they are larger than other printers > but well worth the real estate, in my opinion. Our printers are interfaced > to our A/P LIS (WinSurge), but we also have the ability to print slides and > cassettes as needed through the printing software. Be advised that our > installation required that each printer be connected to its own PC and I > needed to purchase two interfaces from WinSurge, so it wasn't an inexpensive > project by any means. > > I think the Leica labelers are suitable for a high-volume laboratory, but > may be overkill for a lab with smaller volumes. > > Regards, > > Shelley D'Attilio MT(ASCP) > Manager, Chemistry, Cytology and Histology > Dept. of Pathology and Laboratory Medicine > Stormont-Vail HealthCare > Topeka, Kansas > > > NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a > doctor accepting new patients. Call (785) 354-5225. > > > ****************************************************************************************************************** > > The information transmitted in this e-mail and in any replies and forwards > are for the sole use of the above individual(s) or entities and may contain > proprietary, privileged and/or highly confidential information. Any > unauthorized dissemination, review, distribution or copying of these > communications is strictly prohibited. If this e-mail has been transmitted > to you in error, please notify and return the original message to the sender > immediately at the above listed address. Thank you for your cooperation. > > > ****************************************************************************************************************** > > > > ------------------------------ > > Message: 9 > Date: Mon, 17 Oct 2011 09:17:33 -0400 > From: "McMahon, Loralee A" > Subject: [Histonet] HHV-6 Antibody > To: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > < > EC3287D73321A14799BB96082DE57B470DBC9F@URMCMS2.urmc-sh.rochester.edu> > Content-Type: text/plain; charset="iso-8859-1" > > > Hi Histonet. > > Can anyone recommend a HHV-6 antibody to use in formalin fixed paraffin > embedded tissues. > Preferably ASR or IVD. But I'll take whatever suggestions. > Thanks in advance. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > > > ------------------------------ > > Message: 10 > Date: Mon, 17 Oct 2011 07:12:45 -0700 > From: Bea DeBrosse-Serra > Subject: RE: [Histonet] (no subject) > To: "'Miha Tesar'" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <493CAA64F203E14E8823737B9EE0E25F091E912710@EXCHMB01.isis.local> > Content-Type: text/plain; charset="iso-8859-1" > > Do you coat the muscle biopsies with talcum powder prior to freezing? > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 1896 Rutherford Road > Carlsbad, CA 92008 > 760-603-2371 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Miha Tesar > Sent: Saturday, October 15, 2011 1:47 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > Hi! > I am working in Institute of Phatology Slovenia EU. Recently I have > problems with muscle biopsy. There are a lots of artefact that I can not > explain, like little holes in the tissue after the tissue is being frozen. > We are using the isopentan and the liquid nitrogen for the freezing. Can > enyone give me some ideas haw to avoid this artefact. How important is > humidity in laboratory for the good results? > The next problem is that after I put the tissue on the microscope > slides after coloring the GTK all the tissue gets wrinkeld. Iam using the > poly L lisin microscope slides or the Immuno microscope slides. > Thx in advance! > Best regards Miha from SLO > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 95, Issue 22 > **************************************** > From Gudrun.Erikstad <@t> stolav.no Tue Oct 18 01:15:54 2011 From: Gudrun.Erikstad <@t> stolav.no (Erikstad, Gudrun Hovstein) Date: Tue Oct 18 01:16:07 2011 Subject: SV: [Histonet] Re: Looking for recommendations for a slide and cassettelabeller In-Reply-To: Message-ID: We have had a Leica IPC for cassette printing for seven years, and just purchased two more. They are very reliable and the fastest one we've found so far (for batch printing). Works well with LIS (for patient samples) or from excel (for control blocks etc) The downside is that they occupy a lot of space on the bench. A bit noisy too, but I don't know anyone that work more silent. If anyone knows about a cassette printer that is smaller AND can print as fast as the Leica IPC, I would like to know. We haven't tried the Leica IPC for slide printing. Can you use the same printer for both slides and cassettes? (We use labels for slides, but it would be convenient to use the Leica IPC for printing controls slides, as we use the control and the patient section on the same slide. We could then put the patient label over the print) Gudrun, Norway -----Opprinnelig melding----- Fra: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] P? vegne av R C Sendt: 18. oktober 2011 01:19 Til: histonet@lists.utsouthwestern.edu Emne: [Histonet] Re: Looking for recommendations for a slide and cassettelabeller Hi Sheila. As a recent user of the Leica systems for cassette and slide labelling (IP C and S), I recommend them both. The single user console can control both units and although a PC is required you won't be dissatisfied. Stream lining and efficiency seem to suit the units well. Multiple lines of metadata can be written across the blocks as well as the slides. You should look into the units to see if they'd be a fit for your laboratory. On Sun, Oct 16, 2011 at 10:01 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. medi tech users (Sheila Adey) > 2. Looking for recommendations for a slide and cassette labeller > system (Sheila Adey) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 15 Oct 2011 19:55:37 -0400 > From: Sheila Adey > Subject: [Histonet] medi tech users > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Hello: > Can anyone tell me if they know how to assign QC's or Dr.s names to a > batch of cases? We have to go into each case to change a Dr.s name. > I'm also interested in assigning a QC number to a batch of cases with > out having to go into each case. > Thanks in advance. > Sheila > > ------------------------------ > > Message: 2 > Date: Sat, 15 Oct 2011 20:06:19 -0400 > From: Sheila Adey > Subject: [Histonet] Looking for recommendations for a slide and > cassette labeller system > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Hello:We are looking into purchasing a slide and cassette labeller system. > Any advice would be great. Also, if you know which system to stay away > from, that would be appreciated aswell.ThanksSheila > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 95, Issue 21 > **************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nyilmaz <@t> mersin.edu.tr Tue Oct 18 01:22:04 2011 From: nyilmaz <@t> mersin.edu.tr (Nejat Yilmaz) Date: Tue Oct 18 01:21:46 2011 Subject: [Histonet] Trypsin question Message-ID: <36610F9189104B7E9AAC11692B4ECA88@NejatNetbook> Dear Members, Recently we're having some antigen retrieval difficulties with our ihc protocols. My question is; what is 1:250 or 1:300 trypsin (is this dilution or purity rate?) and are these sutiable for antigen retrieval? Which brand or kind of trypsin do you recommend? From mehlikafaire <@t> hotmail.com Tue Oct 18 01:42:54 2011 From: mehlikafaire <@t> hotmail.com (Mehlika Faire) Date: Tue Oct 18 01:42:58 2011 Subject: [Histonet] Trypsin question In-Reply-To: <36610F9189104B7E9AAC11692B4ECA88@NejatNetbook> References: <36610F9189104B7E9AAC11692B4ECA88@NejatNetbook> Message-ID: In my experience with trypsin, the concentration I've used is .05% and .25%. I am not sure how thick or how long your tissues have been fixed-or the type of fixative, but trypsin is really harsh on PFA fixed tissues, so if your tissues are fixed with PFA, I recommend incubating or placed on a slide warmer for atleast an hour. Not sure if you needed the additional info, but just giving you a heads up. Good luck -Mehlika > From: nyilmaz@mersin.edu.tr > To: histonet@lists.utsouthwestern.edu > Date: Tue, 18 Oct 2011 09:22:04 +0300 > Subject: [Histonet] Trypsin question > > Dear Members, > > Recently we're having some antigen retrieval difficulties with our ihc protocols. My question is; what is 1:250 or 1:300 trypsin (is this dilution or purity rate?) and are these sutiable for antigen retrieval? Which brand or kind of trypsin do you recommend? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Catherine.Ross <@t> covance.com Tue Oct 18 06:13:09 2011 From: Catherine.Ross <@t> covance.com (Ross, Catherine) Date: Tue Oct 18 06:13:30 2011 Subject: [Histonet] Acrosome staining (cattle) Message-ID: <1C322956652E7B4681DB8E87252C39721087BC10@harxch01.ent.covance.com> Hi all, Does anyone have a simple method for staining acrosome of cattle sperm, please? I've found a few ideas on Google / Pub Med but would be interested in any specific experience. Thanks in advance! Kind regards, Catherine Dr. Catherine L. Ross DVM MSc(VetPath) MRCVS Covance Laboratories (UK) Ltd. Otley Road, Harrogate, N. Yorks. HG3 1PY T: +44 (0) 1423 848759 E: catherine.ross@covance.com ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From mw <@t> personifysearch.com Tue Oct 18 08:13:07 2011 From: mw <@t> personifysearch.com (Matt Ward) Date: Tue Oct 18 08:13:13 2011 Subject: [Histonet] New Position Alert - Field Histology Specialist - IHC Message-ID: <2f6d9e68f74adba5e91ef23f01951344@mail.gmail.com> Good Morning Histonet! Our client is a world leader in Cancer Diagnostics who is currently seeking Histotechs who have experience working with IHC analyzers that would be interested in Field Support roles. We currently have an opening covering Northern FL, AL, and part of GA. The Position Offers: - Outstanding Base Salary and Competitive Bonus! - Gold Standard Benefits including but not limited to Medical, Cell Phone, Laptop, Car Allowance, Expenses, 401k, Paid Vacation! - Opportunity for Career Advancement! If you are interested in learning more please contact me directly at 800.875.6188 ext. 103 or mw@personifysearch.com Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From Gina.Rodriguez <@t> leica-microsystems.com Tue Oct 18 14:47:32 2011 From: Gina.Rodriguez <@t> leica-microsystems.com (Gina.Rodriguez@leica-microsystems.com) Date: Tue Oct 18 14:47:43 2011 Subject: [Histonet] AUTO: Gina Rodriguez is out of the office. (returning 10/24/2011) Message-ID: I am out of the office until 10/24/2011. I will respond to your message when I return. Note: This is an automated response to your message "Histonet Digest, Vol 95, Issue 23" sent on 10/18/2011 12:01:20 PM. This is the only notification you will receive while this person is away. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From azdudley <@t> hotmail.com Tue Oct 18 16:30:12 2011 From: azdudley <@t> hotmail.com (anita dudley) Date: Tue Oct 18 16:30:17 2011 Subject: [Histonet] cryostats Message-ID: what are people using to disinfect the cryostat, and is everyone taking their machine down weekly as the CAP recomends? thanks for the info anita dudley providence hosp mobile alabama From adesupo2002 <@t> hotmail.com Tue Oct 18 16:43:51 2011 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Tue Oct 18 16:43:55 2011 Subject: [Histonet] Benchmarks for full time histotech Message-ID: Hi, Does anyone know of any national benchmarks for full time histotech?s per case, slide, or specimen? I will appreciate it, if you guys can help me to figure this out. Thanks, Ade. From Diane.Tokugawa <@t> kp.org Tue Oct 18 18:02:27 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Tue Oct 18 18:03:00 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 10/17/2011 and will not return until 10/31/2011. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology issues, please call Mario at 8-421-4961, general histology lab 8-421- 5408 or Wanda at 8-421-5426. From joelleweaver <@t> hotmail.com Tue Oct 18 20:10:07 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Oct 18 20:10:12 2011 Subject: [Histonet] Benchmarks for full time histotech In-Reply-To: References: Message-ID: The numbers that I have been familiar with is about 40 blocks/hour embedding to include large "easy" ones and biopsies, more difficult orientation, including preparation such as cleaning, checking off etc. The cutting is dependant on the complexity of your protocols, but I have seen lab use about 20 slides per/hour with hand slide labeling included, which means also a mix of single section "routine" slides, and also some biopsy, or multiple section slides or levels of increasing depths on single/multiple slides. There are also published workload figures that are searchable and from most browsers, and accessible with registration to the corresponding journals. That being said, I also think that it is most useful to develop in house standards, using as parameters the work load, skill level and speed of your existing staff. The fast/slow are the end points, and an average productivity rate for routine tasks that applies to your specific situation can be developed. Productivity charts help with this, and should be combined with quality technical standards in my opinion to emphasize that speed is not the goal when it is exchanged for quality. Hope this is helpful. Joelle Weaver MAOM, BA, (HTL) ASCP > From: adesupo2002@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 18 Oct 2011 17:43:51 -0400 > Subject: [Histonet] Benchmarks for full time histotech > > > > > Hi, > Does anyone know of any national benchmarks for full time histotech?s per case, slide, or specimen? I will appreciate it, if you guys can help me to figure this out. > > Thanks, > > Ade. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Oct 19 08:23:29 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 19 08:23:34 2011 Subject: [Histonet] Benchmarks for full time histotech In-Reply-To: Message-ID: <1319030609.47293.YahooMailClassic@web65708.mail.ac4.yahoo.com> I am not at home today but tomorrow I will send you the information you need. Ren? J. --- On Tue, 10/18/11, ADESUPO ADESUYI wrote: From: ADESUPO ADESUYI Subject: [Histonet] Benchmarks for full time histotech To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 18, 2011, 5:43 PM Hi, ? ???Does anyone know of any national benchmarks for full time histotech?s per case, slide, or specimen?? I will appreciate it, if you guys can help me to figure this out. ???Thanks, Ade. ??? ???????? ?????? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Oct 19 12:33:23 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Oct 19 12:33:26 2011 Subject: [Histonet] PIG Grippy Mat Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F5CA69@SMCMAIL01.somerset-healthcare.com> I'm trying to find an alternative to the floor mats we have in our histology lab. They get dirty easily, curl, and lift easily at the edges, causing a tripping hazard. Does anyone have experience using these mats? How often do they need to be replaced? Product Description Specially formulated, full coverage adhesive backing holds tight but leaves no residue; eliminates the need for tapes and trays Peels up easily for quick changeouts; reduces cleanup time for a more productive facility Eight layers of fine-fiber polypropylene safely absorb leaks, drips and overspray of oils, coolants, solvents and water to keep liquids from being tracked around No curling or bunching; lies flat to reduce trip hazards around workstations, walkways and machinery Tough, spunbond top layer stands up to foot and light-wheeled traffic as well as heavy equipment driveovers Poly backing creates a liquid-proof barrier to stop absorbed fluids from passing through When used as part of your floor safety program, high-traction surfaces such as PIG(r) Grippy(r) Mat have been proven to reduce slip-and-fall claims by as much as 90% Tested and certified by the National Floor Safety Institute (NFSI) Apply to any clean, dry, oil-free surface; cuts easily with scissors or utility knife Additional Product Information Static Coefficient Of Friction (SCOF) Testing See more PIG(r) Grippy(r) Absorbent Pads & Rolls Warnings & Restrictions Grippy(r) Notice Please note: all adhesives have some potential to lift finish coatings or paint depending on the condition and age of the floor surface. Grippy(r) Mat is formulated to work with most common industrial floor surfaces, but if you have questions about the suitability of Grippy(r) Mat for your application, test a 6" x 6" piece in a noncritical area before use. Available Videos PIG(r) Grippy(r) Mat stays put no matter what! Specifications Color: Gray Dimensions: 25' L x 16" W Mat Weight: Medium-weight Fluids Absorbed: Universal Absorbency: Up to 2 gal. per roll Dispenser: No Perforated: No Sold as: 1 roll Weight: 4.25 lbs. New Pig Patent: Pending # per Pallet: 30 Composition: Absorbent - Polypropylene with Polyester Stitching Backing - Proprietary Grippy(r) Material CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Joyce.Fortin <@t> uhsinc.com Wed Oct 19 13:20:01 2011 From: Joyce.Fortin <@t> uhsinc.com (Fortin, Joyce) Date: Wed Oct 19 13:20:06 2011 Subject: [Histonet] Paraffin Mats Message-ID: Can you please tell me who you order from for paraffin mats and if you like them? Are there pros and cons for any particular sizes and brands? Thank you! Joyce Fortin Histology Supervisor Palmdale Regional Medical Center 38600 Medical Center Drive Palmdale, California 93551 Phone 661-382-5723 Fax 661-382-5747 email: joyce.fortin@uhsinc.com UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. From azdudley <@t> hotmail.com Wed Oct 19 15:40:19 2011 From: azdudley <@t> hotmail.com (anita dudley) Date: Wed Oct 19 15:40:22 2011 Subject: [Histonet] er-pr validation Message-ID: Is there anyone our there that would like to exchange slides or blocks to do er-pr validation? we would be interested. thanks anita dudley 2600 tahoe drive mobile, alabama 251-633-1422 From jzack1967 <@t> yahoo.com Wed Oct 19 16:11:04 2011 From: jzack1967 <@t> yahoo.com (Jackie Zack) Date: Wed Oct 19 16:11:06 2011 Subject: [Histonet] please remove me from the mailing list. thank you Message-ID: <1319058664.42964.YahooMailNeo@web126119.mail.ne1.yahoo.com> From jaylundgren <@t> gmail.com Wed Oct 19 17:19:52 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed Oct 19 17:19:58 2011 Subject: [Histonet] Benchmarks for full time histotech In-Reply-To: <1319030609.47293.YahooMailClassic@web65708.mail.ac4.yahoo.com> References: <1319030609.47293.YahooMailClassic@web65708.mail.ac4.yahoo.com> Message-ID: 30 blocks per hour cutting, 45 blocks/hr embedding. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) From akemiat3377 <@t> yahoo.com Wed Oct 19 18:08:55 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Oct 19 18:08:58 2011 Subject: [Histonet] CAP meeting in Carmel, CA November 4-5 Message-ID: <1319065735.18244.YahooMailNeo@web113805.mail.gq1.yahoo.com> Hi All: ? Just wondering if any of you in histoland are going to the CAP meeting in Carmel at the La Playa in November.? Let me know, I am living in Carmel, and would love to connect.? Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com From pruegg <@t> ihctech.net Wed Oct 19 18:11:28 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Oct 19 18:11:37 2011 Subject: [Histonet] emergency question-IHC interruption In-Reply-To: References: Message-ID: <3541A2211D1F4382921B6C8B0219CC9B@prueggihctechlt> I would leave them in water, don't do the AR because it could reverse sitting around til tomorrow, but depara and sitting in water should be fine, 4dc or even RT. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Thursday, October 13, 2011 2:21 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-request@lists.utsouthwestern.edu Subject: [Histonet] emergency question-IHC interruption Importance: High Hi, Where I can stop my IHC, in what step? I started deparaffinization, but I need to stop and run my IHC tomorrow. Can I leave my slides in 95% Ethanol, or Water or I have to do HIER? Where to store slides: in refrigerator or room. Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From horten_bio <@t> hotmail.com Thu Oct 20 02:29:21 2011 From: horten_bio <@t> hotmail.com (horten ferrero) Date: Thu Oct 20 02:29:34 2011 Subject: [Histonet] (no subject) Message-ID: Histonet mailing list submissions From W.E.J.Hoekert <@t> olvg.nl Thu Oct 20 05:09:08 2011 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Thu Oct 20 05:10:37 2011 Subject: [Histonet] Fatty mamma tissue keeps on washing off References: <14E2C6176416974295479C64A11CB9AE1DECBA0C14@SBS2K8.premierlab.local> Message-ID: <1190CB05C44B13409483514729C2FC3601F841E0@PAIT42.olvg.nl> Hi Histonetters, Every once in a while, we have mamma tissue that washes off the slides completely. The reason for this is that the tissue is still too fat and/or not sufficiently fixed. There is no problem with the HE stain, but the immuno will wash off. What do you do in such cases? I have tried the following: 1: Baking the slides for about 3/4 hours on superfrost slides (that works sometimes but not always) 2: Wood glue slides (that works sometimes but not always) 3:We put the blocks back in hot parraffin for several hours (that usually works) but I was told that this will have a bad influence on the Her2neu stain: a 2+ could become a 1+. I know, the solution is to be sure that the tissue is fixed long enough and that the fat has been removed sufficiently using acetone, but sometimes that is just not the case. How do you cope with this? Willem Hoekert PA-lab OLVG Amsterdam The Netherlands Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From one_angel_secret <@t> yahoo.com Thu Oct 20 07:25:53 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Oct 20 07:25:57 2011 Subject: [Histonet] Fatty mamma tissue keeps on washing off In-Reply-To: <1190CB05C44B13409483514729C2FC3601F841E0@PAIT42.olvg.nl> References: <14E2C6176416974295479C64A11CB9AE1DECBA0C14@SBS2K8.premierlab.local> <1190CB05C44B13409483514729C2FC3601F841E0@PAIT42.olvg.nl> Message-ID: <1319113553.96920.YahooMailNeo@web112309.mail.gq1.yahoo.com> Well, I never thought I would be raving about a slide, but that day has arrived. Not long ago I got my first encounter with the Dako IHC slides. They are great! I dont know how they are made but they were able to keep on breast cases for me when nothing else worked. I had a couple of other cases that were bloody and wanted to fall off too, they worked. I highly recommend these slides. ( along with getting your fixation issue resolved) :) Kim ________________________________ From: "Hoekert, W.E.J." To: histonet@lists.utsouthwestern.edu Sent: Thursday, October 20, 2011 6:09 AM Subject: [Histonet] Fatty mamma tissue keeps on washing off Hi Histonetters, Every once in a while, we have mamma tissue that washes off the slides completely. The reason for this is that the tissue is still too fat and/or not sufficiently fixed. There is no problem with the HE stain, but the immuno will wash off. What do you do in such cases? I have tried the following: 1: Baking the slides for about 3/4 hours on superfrost slides (that works sometimes but not always) 2: Wood glue slides (that works sometimes but not always) 3:We put the blocks back in hot parraffin for several hours (that usually works) but I was told that this will have a bad influence on the Her2neu stain: a 2+ could become a 1+. I know, the solution is to be sure that the tissue is fixed long enough and that the fat has been removed sufficiently using acetone, but sometimes that is just not the case. How do you cope with this? Willem Hoekert PA-lab OLVG Amsterdam The Netherlands Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Thu Oct 20 07:40:32 2011 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Thu Oct 20 07:40:41 2011 Subject: [Histonet] DRYING TIMES for immuno stains Message-ID: Can you tell me how long you let your IHC slides air dry at room temperature and how long you put them in the oven prior to staining? Thanks Diana From minniesann <@t> hotmail.com Thu Oct 20 09:34:15 2011 From: minniesann <@t> hotmail.com (Maribel Santiago) Date: Thu Oct 20 09:34:18 2011 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: > From: horten_bio@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 20 Oct 2011 09:29:21 +0200 > Subject: [Histonet] (no subject) > > > > > > Histonet mailing list submissions > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Thu Oct 20 10:00:27 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Oct 20 10:00:38 2011 Subject: [Histonet] RE: DRYING TIMES for immuno stains In-Reply-To: References: Message-ID: Air dry over air vent for a minimum of 30 min Into 60C oven for 15 min Direct transfer to hot water-detergent bath for de-wax Tresa Goins Veterinary Diagnostic Lab South 19th and Lincoln Bozeman, MT 59718 406-994-6353 - phone 406-994-6344 - fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Thursday, October 20, 2011 6:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DRYING TIMES for immuno stains Can you tell me how long you let your IHC slides air dry at room temperature and how long you put them in the oven prior to staining? Thanks Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Oct 20 10:22:32 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 20 10:22:36 2011 Subject: [Histonet] DRYING TIMES for immuno stains In-Reply-To: Message-ID: <1319124152.18122.YahooMailClassic@web65702.mail.ac4.yahoo.com> I always placed the slides?in an oven at 60?C for 30 minutes?immediately after they were cut. Just make sure there is no water left behind the section when they go into the oven. Ren? J.?? --- On Thu, 10/20/11, Diana McCaig wrote: From: Diana McCaig Subject: [Histonet] DRYING TIMES for immuno stains To: histonet@lists.utsouthwestern.edu Date: Thursday, October 20, 2011, 8:40 AM Can you tell me how long you let your IHC slides air dry at room temperature? and how long you put them in the oven prior to staining? Thanks Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapcl <@t> yahoo.com Thu Oct 20 10:57:33 2011 From: chapcl <@t> yahoo.com (William) Date: Thu Oct 20 10:57:39 2011 Subject: [Histonet] DRYING TIMES for immuno stains In-Reply-To: <1319124152.18122.YahooMailClassic@web65702.mail.ac4.yahoo.com> References: <1319124152.18122.YahooMailClassic@web65702.mail.ac4.yahoo.com> Message-ID: Dr. David Tacha at Biocare Medical has done extensive research on this very topic. His findings confirm that the best results (both tissue staying on the slides and IHC reactivity) occur by drying at 37 degrees for 30 minutes to remove any water behind the section slowly, followed by 60 degrees for 30 minutes. Longer times at 60 degrees is just as detrimental as shorter times. Hope that helps. Biocare technical support may have copies of his research. Will Chappell Sent from my iPhone On Oct 20, 2011, at 8:22 AM, Rene J Buesa wrote: > I always placed the slides in an oven at 60?C for 30 minutes immediately after they were cut. Just make sure there is no water left behind the section when they go into the oven. > Ren? J. > > --- On Thu, 10/20/11, Diana McCaig wrote: > > > From: Diana McCaig > Subject: [Histonet] DRYING TIMES for immuno stains > To: histonet@lists.utsouthwestern.edu > Date: Thursday, October 20, 2011, 8:40 AM > > > Can you tell me how long you let your IHC slides air dry at room > temperature and how long you put them in the oven prior to staining? > > > > Thanks > > Diana > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From duffettc <@t> sjhmc.org Thu Oct 20 11:21:18 2011 From: duffettc <@t> sjhmc.org (Duffett, Christopher) Date: Thu Oct 20 11:22:06 2011 Subject: [Histonet] ANP 22969 Message-ID: <3AC59012F7CF5F499F6C5718F0C16DB404B0B361@itr-exch01.sjhmc.sjhealthsys.org> I am looking for information on Cap question ANP22969 which is "For IHC and Fish/ISH studies that provide independent predictive information, the patients report includes information on specimen processing, the antibody clone/probe and the scoring method used." Has anyone come up with a canned message or can give me some input on how to set this up. Your help and comments would be appreciated Chris Duffett Pathology Manager St. Joseph's Regional Medical Center 703 Main Street Paterson, NJ 07503 Office 973-754-3541 Fax 973-754-3649 duffettc@sjhmc.org CONFIDENTIAL COMMUNICATION: THIS TRANSMISSION IS INTENDED ONLY FOR THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND CONTAINS INFORMATION THAT IS CONFIDENTIAL. IF YOU HAVE RECEIVED THIS COMMUNICATION IN ERROR, PLEASE DELETE THE EMAIL AND CONTACT THE SENDER IMMEDIATELY. THIS INFORMATION MAY HAVE BEEN DISCLOSED TO YOU FROM CONFIDENTIAL RECORDS AND MAY BE PROTECTED BY FEDERAL AND STATE LAW. THIS INFORMATION MAY INCLUDE CONFIDENTIAL MENTAL HEALTH, SUBSTANCE ABUSE, ALCOHOL ABUSE AND/OR HIV-RELATED INFORMATION. FEDERAL AND STATE LAW PROHIBITS YOU FROM MAKING ANY FURTHER DISCLOSURE OF THIS INFORMATION WITHOUT THE SPECIFIC WRITTEN CONSENT OF THE PERSON TO WHOM IT PERTAINS, OR AS OTHERWISE PERMITTED BY LAW. ANY UNAUTHORIZED FURTHER DISCLOSURE IN VIOLATION OF THE LAW MAY RESULT IN A FINE OR JAIL SENTENCE OR BOTH. A GENERAL AUTHORIZATION FOR THE RELEASE OF THIS INFORMATION MAY NOT BE SUFFICIENT AUTHORIZATION FOR FURTHER DISCLOSURE. From mfisher <@t> ecrmc.org Thu Oct 20 12:32:44 2011 From: mfisher <@t> ecrmc.org (Marcia Fisher) Date: Thu Oct 20 12:32:53 2011 Subject: [Histonet] Grippy Mat Message-ID: Those sound like the same ones we had in our core clinical lab which I tripped on and fell, breaking my right humeral head 11 weeks ago! All mats of that type are now gone and replaced with a much thicker, heavier and anti-microbial. Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center 1415 Ross Ave El Centro, CA 92243 760-339-7267 760-482-5365(F) www.ecrmc.org Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.   From nicole <@t> dlcjax.com Thu Oct 20 13:27:50 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Thu Oct 20 13:38:12 2011 Subject: [Histonet] Histoville Message-ID: <1065.208.62.167.196.1319135270.squirrel@webmail.realpages.com> To all of you out there who dont already know, Ive created a facbook page called Histoville. It's a great place to ask for help, meet friends and discuss any issues relating to our field. We have already almost 300 members. I hope you will come and join us and help grow our community page. I look forward to making new histo friends. Nicole Tatum, HT ASCP From mtitford <@t> aol.com Thu Oct 20 15:26:25 2011 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Thu Oct 20 15:26:35 2011 Subject: [Histonet] AFIP History Message-ID: <8CE5D7DAC4EBBB2-21F0-1D0B4@webmail-d182.sysops.aol.com> Those of you who mourn the passing of the AFIP may be interested that a book has been published about its history, entitled, "Legacy of Excellance: The Armed Forces Institute of Pathology 1862 - 2011" by Paul Stone, published by The Borden Institute, Fort Detrick, Maryland. It details the AFIP history from the Civil War period to the present. It has many, many great photographs. The early chapters are mainly about medical care in the Army during the early years, but later chapters are about its different missions in later years. There are photographs of the museum and its different specimens, teaching of pathologists, research, DNA, veterinary, forensic, etc, etc. Photographs include those of Frank Avalone, Dr Frank Johnson and Lee Luna who all devised histology staining methods. Lee Luna is described as "The Father of Histology" (He may have some competition for that title!). Dr Johnson is shown using what I think is an RCA EMU 3 transmission electron microscope, the only TEM made in the USA (I think). The National Society of Histotechnology is mentioned too. I worked at the AFIP for a few weeks on two occasions. It was interesting to see some familier faces in the book. Michael Titford USA Pathology Mobile AL From jkiernan <@t> uwo.ca Thu Oct 20 23:45:26 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Oct 20 23:45:29 2011 Subject: [Histonet] AFIP History In-Reply-To: <8CE5D7DAC4EBBB2-21F0-1D0B4@webmail-d182.sysops.aol.com> References: <8CE5D7DAC4EBBB2-21F0-1D0B4@webmail-d182.sysops.aol.com> Message-ID: <75d0853b45fa8.4ea0c0a6@uwo.ca> Well posted, Michael Titford! Americans need the occasional expat Brit to remind them tht they have some history. John Kiernan Anatomy, UWO London, Canada = = = On 20/10/11, mtitford@aol.com wrote: > > > Those of you who mourn the passing of the AFIP may be interested that a book has been published about its history, entitled, "Legacy of Excellance: The Armed Forces Institute of Pathology 1862 - 2011" by Paul Stone, published by The Borden Institute, Fort Detrick, Maryland. It details the AFIP history from the Civil War period to the present. It has many, many great photographs. The early chapters are mainly about medical care in the Army during the early years, but later chapters are about its different missions in later years. There are photographs of the museum and its different specimens, teaching of pathologists, research, DNA, veterinary, forensic, etc, etc. > > Photographs include those of Frank Avalone, Dr Frank Johnson and Lee Luna who all devised histology staining methods. Lee Luna is described as "The Father of Histology" (He may have some competition for that title!). Dr Johnson is shown using what I think is an RCA EMU 3 transmission electron microscope, the only TEM made in the USA (I think). The National Society of Histotechnology is mentioned too. > > I worked at the AFIP for a few weeks on two occasions. It was interesting to see some familier faces in the book. > > Michael Titford > USA Pathology > Mobile AL > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From histopath101 <@t> gmail.com Fri Oct 21 08:43:47 2011 From: histopath101 <@t> gmail.com (histopath101@gmail.com) Date: Fri Oct 21 08:43:56 2011 Subject: [Histonet] ? on frozen tissue artifacts Message-ID: <001636b2b57a3b04d204afcf434e@google.com> I work in a reference lab and some of our frozen sections exhibit severe freeze artifact (ice crystals). The client claims it's something we're doing and we say they are not freezing them properly. We receive the specimens on dry ice and store them in a -20C freezer until we section them (no more than 1-2 days later). We never allow the tissues to thaw. My question: Can freeze artifact occur AFTER properly snap-freezing tissue or does this artifact ONLY occur during the initial preezing procedure? From melissa <@t> alliedsearchpartners.com Fri Oct 21 08:47:45 2011 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Fri Oct 21 08:47:49 2011 Subject: [Histonet] Two Histology Supervisors needed in FL-Two Different HOT JOBS Message-ID: Allied Search Partners is currently looking for two qualified histology supervisors for two different open jobs in Fort Myers, FL. The first one listed below is for a reference laboratory and the second one listed below is with a private laboratory. Please let me know by sending your resume if you are interested. At that time I will give you a call to speak more in depth about the job. Thank you! Melissa@alliedsearchpartners.com ______________________________________________________________________________ Position: Histology Supervisor Schedule: Monday-Friday, Full Time Location: Fort Myers Reference Laboratory Setting Summary: Supervisor the daily routine and specialized histology techniques and procedures. Supervision of Embedding, Microtomy, Grossing, Processing, and H&E staining Special Staining and Equipment maintenance Requirements: FL Supervisor Laboratory License Previous experience with automated IHC Technical and QC protocols AA or BS/BA degree in life science preferred but not required Benefits: Competitive salaries, Health, Dental, Life & Disability insurances, a section 125 plan, a 401K, FSA, ESPP and relocation assistance. ------------------------------ *Position Title: *Histology Supervisor *Reports To: *Laboratory Director *Shift: *Monday-Friday, 8am-5pm * * *Location: Private* Pathology Laboratory for well established busy office in Fort Myers, FL founded in 2000. * * *Requirements:* * * - ASCP certification preferred - Experience leading a team of 3+ laboratory staff memebers** - Experience and knowledge of OSHA, CLIA, AHCA regulations** - The ability to implement and maintain QA/QC policies and procedures** - Oversee safety and training** - Understanding of the different lab licensing and ranges of testing** * * *Summary:* * * - Responsible for 2 other employees within the laboratory** - Work with the Director of Compliance on compliancy of the lab** - Handles all laboratory compliance** - Occasionally help with day to day routine histology** * * * * *Benefits:* Health Insurance, Dental Insurance, Vision Insurance. Group Life, Short Term Disability and Long Term Disability paid by employer. Voluntary Life for self, spouse, and child. Discount on employer products and services. PTO for vacation, personal time, sick etc. Paid Holidays (7): New Years Day, Memorial Day, Fourth of July, Labor Day, Thanksgiving Day, Day after Thanksgiving Day, and Christmas Day. Bereavement Leave.* *Bi-annual bonuses, performance reviews, uniform and name badge. Direct Depost & 401K -- Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Lab Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com From Allison_Scott <@t> hchd.tmc.edu Fri Oct 21 09:00:44 2011 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Oct 21 09:00:53 2011 Subject: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus Message-ID: Hello to all in histo land. For those of you that have the ventana special stains machine, can the alcian blue 2.5 be done on the machine? I am not sure if they have kits for this stain. Also is anyone using the cryoembedding apparatus from pathology innovations? Thanks in advance. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From Barry.R.Rittman <@t> uth.tmc.edu Fri Oct 21 09:03:11 2011 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Oct 21 09:03:14 2011 Subject: [Histonet] ? on frozen tissue artifacts In-Reply-To: <001636b2b57a3b04d204afcf434e@google.com> References: <001636b2b57a3b04d204afcf434e@google.com> Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E901926928ED@UTHCMS1.uthouston.edu> Hi Even if you store frozen blocks and sections in a freezer no matter what temperature (even to storing in liquid nitrogen) tissues can still dry out and show various artifacts. This occurs more rapidly with minus 20 than when storing at lower temperatures. Also some freezers automatically defrost, assume your does not. Might I suggest that you check to ensure your freezer does not defrost, and in any case store your sections in a closed container with a few ice cubes - not in contact with tissues of course, this will prevent them drying out. As you note, artifacts cam also be introduced with the initial freezing, depending on the method of freezing, tissue thickness etc. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histopath101@gmail.com [histopath101@gmail.com] Sent: Friday, October 21, 2011 8:43 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] ? on frozen tissue artifacts I work in a reference lab and some of our frozen sections exhibit severe freeze artifact (ice crystals). The client claims it's something we're doing and we say they are not freezing them properly. We receive the specimens on dry ice and store them in a -20C freezer until we section them (no more than 1-2 days later). We never allow the tissues to thaw. My question: Can freeze artifact occur AFTER properly snap-freezing tissue or does this artifact ONLY occur during the initial preezing procedure? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Fri Oct 21 09:15:24 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Oct 21 09:15:30 2011 Subject: [Histonet] UAMS Looking for a Histotech Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32096B721A@Mail2Node2.ad.uams.edu> Good Morning, We are looking for a register Histologist for a fulltime job at the University of Arkansas for Medical Sciences in Little Rock. We would prefer someone for 3rd shift however, it is negotiable for rotation every two to three months. The pay is hourly with a shift differential for nights and competitive for the area. We have great equipment with more changes coming. This is a chance to learn and grow with the Histology department at UAMS. The benefits are great and the retirement is a matching up to 10% for your contributions by UAMS. We have 11 paid holidays each year and two weeks of vacation with sick time allotted per month. We cannot pay relocation or bonuses as this is a state run University site and we cannot use a recruiter, so please do not ask. I have tried and it is no recruiters. Please see the UAMS website for Jobs under #5003810 and submit a resume. You may also call me for details or other information however: I cannot officially interview anyone without a submission through HR. Best Regards, Pamela A Marcum AP Supervisor Histology Slot 502 4301 W Markham Street Little Rock AR 72205 Office: 501-686-7554 Fax: 501-686-7151 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From gu.lang <@t> gmx.at Fri Oct 21 10:32:41 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Oct 21 10:32:50 2011 Subject: AW: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus In-Reply-To: References: Message-ID: Ventana has a kit for alcian blue. I wasn't very satisfied with this kit. The staining was faint in comparison to manual staining. Staining temp is at 60 degrees. I'm not sure how the temp influences exactly the pH of the staining solution, but it may decrease it under the wanted 2,5. So the result is rather a pH1 than pH2,5 alcian blue. That are just theoretic considerations, never checked. - what do you think? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Scott, Allison D Gesendet: Freitag, 21. Oktober 2011 16:01 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus Hello to all in histo land. For those of you that have the ventana special stains machine, can the alcian blue 2.5 be done on the machine? I am not sure if they have kits for this stain. Also is anyone using the cryoembedding apparatus from pathology innovations? Thanks in advance. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa <@t> alliedsearchpartners.com Fri Oct 21 14:31:25 2011 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Fri Oct 21 14:31:29 2011 Subject: [Histonet] NY Licensed Grossing Technician Needed in NY-3rd Shift-HOT JOB Message-ID: Allied Search Partners is currently looking for a qualified, NY Licensed, Grossing Technician or Technologist to work the 3rd shift (overnight hours). * * *Position Name:** *Grossing Technician *Location:* Port Chester, NY area *Schedule:* Full Time/Permanent 3rd shift (overnight hours) also accepting Part Time applicants for Overnight Shift *Summary of Dutes: * 1. Ensure specimen integrity upon arrival and through out the entire process. 2. Dictate a gross description for each biopsy specimen, dissect and prepare tissue for cassettes. 3. Gross core needle biopsy and all anatomical pathology specimens (under the supervision of a Pathologist Assistant and /or pathologist). 4. Prepare dissected tissue specimens and place into cassettes. 5. Load prepared cassettes in Tissue Processors. 6. Operation of all tissue processors. 7. Compliance with all Federal and State regulations pertaining to Grossing: 1. Adhere to workload requirements. 2. Maintenance of accurate work records (i.e., Maintenance Logs, Filter Logs, Bench Excerpts etc.). 8. Able to evaluate new laboratory equipment and procedures. 9. Able to perform Quality Control and Quality Assurance Procedures. 10. Working knowledge and understanding of TPS / Lab Manager IS System. *Requirements:* NYS License Associated Degree in Biology or Science *Benefits:* Benefits Enrollment and Eligibility Medical Benefits Dental Benefits Vision Benefits Short-Term Disability Long-Term Disability Life/ Accidental Death & Dismemberment Supplemental Life Insurance Business Travel Accident Insurance Employee Assistance Program Other Available Benefits Personal Time Off Vacation Holiday Schedule Bereavement Leave Tuition Reimbursement 401 (k) Savings Plan *To Apply Please:* 1. Please email a copy of updated resume to melissa@alliedsearchpatners.com 2. Please send availability for a phone screen with one of our recruiters. 3. Please send salary ranges -- Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Lab Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com From SEsparza <@t> seton.org Fri Oct 21 15:07:21 2011 From: SEsparza <@t> seton.org (Esparza, Sandra) Date: Fri Oct 21 15:07:28 2011 Subject: [Histonet] ? on frozen tissue artifacts In-Reply-To: <001636b2b57a3b04d204afcf434e@google.com> References: <001636b2b57a3b04d204afcf434e@google.com> Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB041DFE5A@AUSEX2VS1.seton.org> I have never put my muscles in a -20 we use -70 to -80 freezer and have not had a problem with artifact. Is your freezer frost free? Sandra Sandra Esparza HT(ASCP)QIHC Lead Technologist Dell Children's Medical Center of Central Texas 512-324-0000 x87061 sesparza@seton.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histopath101@gmail.com Sent: Friday, October 21, 2011 8:44 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] ? on frozen tissue artifacts I work in a reference lab and some of our frozen sections exhibit severe freeze artifact (ice crystals). The client claims it's something we're doing and we say they are not freezing them properly. We receive the specimens on dry ice and store them in a -20C freezer until we section them (no more than 1-2 days later). We never allow the tissues to thaw. My question: Can freeze artifact occur AFTER properly snap-freezing tissue or does this artifact ONLY occur during the initial preezing procedure? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From charleso.o606 <@t> gmail.com Fri Oct 21 16:00:14 2011 From: charleso.o606 <@t> gmail.com (Charles O) Date: Fri Oct 21 16:00:18 2011 Subject: [Histonet] Seekin Histo/IHC POSITION Message-ID: Hi, I am a certified histotech seeking a new position. I have an extensive experience in the general histological technics and in Immunohistochemistry. I am willing to relocate. Thanks, Charles O. From Vickroy.Jim <@t> mhsil.com Fri Oct 21 16:32:44 2011 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Oct 21 16:32:53 2011 Subject: [Histonet] Billing for Surgical Pathology Message-ID: <24A4826E8EF0964D86BC5317306F58A55FD2208E53@mmc-mail.ad.mhsil.com> Billing question - I have read and understand the CPT codes for surgical pathology specimens including bundling, etc. I also understand that we can bill a CPT code for each separate specimen. (I know there may be exceptions.) What I don't clearly understand is the "Modifier" side of all of this. Example: Let's say we have four gi biopsies (asc. Colon bx, desc colon bs, esophageal bx, and a rectal bx. In our system we would enter 4 - 88305's. Where the argument and question has come is that do we have to add a modifier to these specimens since there are multiple specimens? Some sources we have say that since they are separately submitted specimens that a modifier is not needed. Someone else today said that the first of the four specimens does not need a modifier but the remaining four do. Can anyone shed some light on this? We have counted on our billing department to be the specialists on this stuff but now that is in question. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From JWeems <@t> sjha.org Fri Oct 21 16:41:22 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Oct 21 16:41:27 2011 Subject: [Histonet] RE: Billing for Surgical Pathology In-Reply-To: <24A4826E8EF0964D86BC5317306F58A55FD2208E53@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A55FD2208E53@mmc-mail.ad.mhsil.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640828E5C233@CHEXCMS10.one.ads.che.org> This is my understanding.. I think it is meant for clinical tests, but is used for AP also. You have to use modifier 91 for each specimen in addition to the first one removed on the same day. Part A would be 88305, the others would be 88305-91 so that multiples of the same CPT code can be billed. Here is a web site that has info. It is from 2007, but still relevant, I think. http://health-information.advanceweb.com/article/brush-up-on-cpthcpcs-modifiers.aspx And it's description of 91 'Modifier -91 is appended to repeat clinical diagnostic laboratory tests performed on the same date. Modifier -91 is not to be used for procedures repeated to verify results or due to equipment failure or specimen inadequacy. If the same test was performed on different sites, use modifier -59 instead. For example, if two wound cultures were taken from two different wound sites, modifier -59 would be appended to the second wound culture code. However, if a second culture was taken of the same wound site, then it would be appropriate to append modifier -91 to the second wound culture code. If a lab panel is performed and one of the tests within the panel is repeated, modifier -91 is appended to the repeat lab test. " Have a great weekend everyone!! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Friday, October 21, 2011 17:33 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing for Surgical Pathology Billing question - I have read and understand the CPT codes for surgical pathology specimens including bundling, etc. I also understand that we can bill a CPT code for each separate specimen. (I know there may be exceptions.) What I don't clearly understand is the "Modifier" side of all of this. Example: Let's say we have four gi biopsies (asc. Colon bx, desc colon bs, esophageal bx, and a rectal bx. In our system we would enter 4 - 88305's. Where the argument and question has come is that do we have to add a modifier to these specimens since there are multiple specimens? Some sources we have say that since they are separately submitted specimens that a modifier is not needed. Someone else today said that the first of the four specimens does not need a modifier but the remaining four do. Can anyone shed some light on this? We have counted on our billing department to be the specialists on this stuff but now that is in question. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From one_angel_secret <@t> yahoo.com Fri Oct 21 17:05:45 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Oct 21 17:05:50 2011 Subject: [Histonet] RE: Billing for Surgical Pathology In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640828E5C233@CHEXCMS10.one.ads.che.org> References: <24A4826E8EF0964D86BC5317306F58A55FD2208E53@mmc-mail.ad.mhsil.com> <92AD9B20A6C38C4587A9FEBE3A30E1640828E5C233@CHEXCMS10.one.ads.che.org> Message-ID: <1319234745.55179.YahooMailNeo@web112315.mail.gq1.yahoo.com> Thats my understanding as well. I must say though when it comes?to compliance issues such as billing. Dont go it alone. If you cant count on your billing department I HIGHLY recomend you sign up for "Code Correct". This is the group Ive always used. Great soft ware, seminars, website and they send updates to your email. No, I dont work for them lol . Just know how good they are. Who ever you choose, and you should choose someone because you need a tool that is accurate. Here's the link: http://www.grouponehealthsource.com/code-correct/ ? Best Regards ? Kim :D ________________________________ From: "Weems, Joyce" To: "Vickroy, Jim" ; "histonet@lists.utsouthwestern.edu" Sent: Friday, October 21, 2011 5:41 PM Subject: [Histonet] RE: Billing for Surgical Pathology This is my understanding.. I think it is meant for clinical tests, but is used for AP also. You have to use modifier 91 for each specimen in addition to the first one removed on the same day.? Part A would be 88305, the others would be 88305-91 so that multiples of the same CPT code can be billed. Here is a web site that has info. It is from 2007, but still relevant, I think. http://health-information.advanceweb.com/article/brush-up-on-cpthcpcs-modifiers.aspx? And it's description? of 91 'Modifier -91 is appended to repeat clinical diagnostic laboratory tests performed on the same date. Modifier -91 is not to be used for procedures repeated to verify results or due to equipment failure or specimen inadequacy. If the same test was performed on different sites, use modifier -59 instead. For example, if two wound cultures were taken from two different wound sites, modifier -59 would be appended to the second wound culture code. However, if a second culture was taken of the same wound site, then it would be appropriate to append modifier -91 to the second wound culture code. If a lab panel is performed and one of the tests within the panel is repeated, modifier -91 is appended to the repeat lab test. " Have a great weekend everyone!! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Friday, October 21, 2011 17:33 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing for Surgical Pathology Billing question -? I have read and understand the CPT codes for surgical pathology specimens including bundling, etc.? I also understand that we can bill a CPT code for each separate specimen. (I know there may be exceptions.) What I don't clearly understand is the "Modifier" side of all of this.? Example: Let's say we have four gi biopsies (asc. Colon bx, desc colon bs, esophageal bx, and a rectal bx. In our system we would enter 4 - 88305's.? Where the argument and question has come is that do we have to add a modifier to these specimens since there are multiple specimens? Some sources we have say that since they are separately submitted specimens that a modifier is not needed.? Someone else today said that the first of the four specimens does not need a modifier but the remaining four do. Can anyone shed some light on this?? We have counted on our billing department to be the specialists on this stuff but now that is in question. Jim James Vickroy BS, HT(ASCP) Surgical? and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Fri Oct 21 18:01:09 2011 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri Oct 21 18:01:23 2011 Subject: [Histonet] out of office Message-ID: I will be out of the office starting 10/21/2011 and will not return until 10/24/2011. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. From charleso.o606 <@t> gmail.com Sat Oct 22 06:59:53 2011 From: charleso.o606 <@t> gmail.com (Charles O) Date: Sat Oct 22 06:59:56 2011 Subject: [Histonet] Re: Seekin Histo/IHC POSITION In-Reply-To: References: Message-ID: On Fri, Oct 21, 2011 at 4:00 PM, Charles O wrote: > > > Hi, > I am a certified histotech seeking a new position. I have an > extensive experience in the general histological technics and in > Immunohistochemistry. I am willing to relocate. > > Thanks, > > Charles O. > From kjgada <@t> gmail.com Sat Oct 22 08:46:00 2011 From: kjgada <@t> gmail.com (Komal Gada) Date: Sat Oct 22 08:46:05 2011 Subject: [Histonet] Suggestions please... Message-ID: Dear Histonetters, I'm currently teaching Histology at a University, and I was hoping for some suggestions on how to teach students to use a cryostat. I have several questions: 1) Since we do not have access to actual specimens, what would any of you recommend could be used as a viable option? So far, I'm thinking either hot dogs or chicken breasts, but please feel free to suggest what you think and why so that I can understand the logic. 2) Which post-fixative should I use and how long? 3) Are there any suggestions for the H&E staining procedure? Thanks! Komal From Ronald.Houston <@t> nationwidechildrens.org Sat Oct 22 10:19:07 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Sat Oct 22 10:19:16 2011 Subject: [Histonet] TGF beta Message-ID: Having some trouble getting any staining with AbCam's TGF beta antibody? Any recommendations Thanks Ronnie Houston ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From pruegg <@t> ihctech.net Sat Oct 22 13:14:11 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Oct 22 13:14:22 2011 Subject: [Histonet] MDR3 Message-ID: <2CD65D58F6B1434F8CE17C156928BCE4@prueggihctechlt> Does anyone have working protocol for Multi Drug Resistant 3 abs on ffpe human and/or rat placenta? If you have this working please tell me what ab and protocol you may be using. Thank you in advance, Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org From pruegg <@t> ihctech.net Sat Oct 22 15:14:32 2011 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sat Oct 22 15:14:38 2011 Subject: [Histonet] TGF beta Message-ID: <20111022131432.f86bd30e73b823f57b516b5451216a98.4dbd1fb48d.wbe@email01.secureserver.net> i use TGFb from Peprotech -------- Original Message - Subject: [Histonet] TGF beta From: "Houston, Ronald" gmail.com Sat Oct 22 16:05:36 2011 From: histopath101 <@t> gmail.com (histopath101@gmail.com) Date: Sat Oct 22 16:05:40 2011 Subject: [Histonet] ? on frozen tissue artifacts In-Reply-To: <3D79F47DC92B204F9E5D35C885DFC5CB041DFE5A@AUSEX2VS1.seton.org> Message-ID: I never said these were muscles. These are tumors removed and frozen the day before and sent to us on dry ice. We know not to use frost-free freezers! On , "Esparza, Sandra" wrote: > I have never put my muscles in a -20 we use -70 to -80 freezer and have > not had a problem with artifact. Is your freezer frost free? > Sandra > Sandra Esparza HT(ASCP)QIHC > Lead Technologist > Dell Children's Medical Center of Central Texas > 512-324-0000 x87061 > sesparza@seton.org > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > histopath101@gmail.com > Sent: Friday, October 21, 2011 8:44 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] ? on frozen tissue artifacts > I work in a reference lab and some of our frozen sections exhibit severe > freeze artifact (ice crystals). The client claims it's something we're > doing and we say they are not freezing them properly. We receive the > specimens on dry ice and store them in a -20C freezer until we section > them > (no more than 1-2 days later). We never allow the tissues to thaw. My > question: Can freeze artifact occur AFTER properly snap-freezing tissue > or > does this artifact ONLY occur during the initial preezing procedure? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Sat Oct 22 16:33:40 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sat Oct 22 16:33:44 2011 Subject: [Histonet] ? on frozen tissue artifacts In-Reply-To: References: <3D79F47DC92B204F9E5D35C885DFC5CB041DFE5A@AUSEX2VS1.seton.org> Message-ID: <1319319220.6870.YahooMailNeo@web112304.mail.gq1.yahoo.com> Something Ive seen is there are labs out in histoland who just toss that tissue in a bag, put it on ice and think all is well. If this is what your clients are doing then they need to be educated on how to prepare them.Because if this is what they are doing common sense should tell them tissue has water in it, a slow freeze will create more?artifacts.?Even if they are snap freezing the specimen with alcohol and a dry ice slush, which is better than just putting it in dry ice, some artifacts could happen( I think isopentane and lq nitrogen is still the best method for any tissues imho). The only other things I can think is if somewhere in the process the specimen thaws. Also, without trying to be too much of a wind bag lol...... can you give us the exact procedure your clients have been given by you to snap freeze thier specimens? That might help us help you :) ? Kim :D ________________________________ From: "histopath101@gmail.com" To: "Esparza, Sandra" ; histopath101@gmail.com Cc: Histonet@lists.utsouthwestern.edu Sent: Saturday, October 22, 2011 5:05 PM Subject: Re: RE: [Histonet] ? on frozen tissue artifacts I never said these were muscles. These are tumors removed and frozen the day before and sent to us on dry ice. We know not to use frost-free freezers! On , "Esparza, Sandra" wrote: > I have never put my muscles in a -20 we use -70 to -80 freezer and have > not had a problem with artifact. Is your freezer frost free? > Sandra > Sandra Esparza HT(ASCP)QIHC > Lead Technologist > Dell Children's Medical Center of Central Texas > 512-324-0000 x87061 > sesparza@seton.org > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > histopath101@gmail.com > Sent: Friday, October 21, 2011 8:44 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] ? on frozen tissue artifacts > I work in a reference lab and some of our frozen sections exhibit severe > freeze artifact (ice crystals). The client claims it's something we're > doing and we say they are not freezing them properly. We receive the > specimens on dry ice and store them in a -20C freezer until we section > them > (no more than 1-2 days later). We never allow the tissues to thaw. My > question: Can freeze artifact occur AFTER properly snap-freezing tissue > or > does this artifact ONLY occur during the initial preezing procedure? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sun Oct 23 04:53:36 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Oct 23 04:53:43 2011 Subject: AW: [Histonet] Suggestions please... In-Reply-To: References: Message-ID: I suggest fresh pig-kidney, if you can get it at the butcher. Kidney is relatively easy to cut and you can prepare small quaders of 2-3 mm thickness, that are frozen fast enough directly in the cryocut. After cutting you can wrap it in foil and store it in the fridge (-20?C) for the next students. For teaching I think air drying is good enough before HE-staining. Alternatively you can take 37-40% formaldehyde for a few seconds and rinsing in tapwater afterwards. Then 1 min Harris hematoxylin Rinse in acidified water (dest. water + 1 drop acetic acid) Rinse in tapwater until blue enough Rinse in 96% ethanol 15-30 seconds in 2% eosin in 96% ethanol Rinse in 96% ethanol 2 x rinse in absolute ethanol 1 x rinse in xylol or substitute Coverslipping with resin-media Hope that helps Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Komal Gada Gesendet: Samstag, 22. Oktober 2011 15:46 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Suggestions please... Dear Histonetters, I'm currently teaching Histology at a University, and I was hoping for some suggestions on how to teach students to use a cryostat. I have several questions: 1) Since we do not have access to actual specimens, what would any of you recommend could be used as a viable option? So far, I'm thinking either hot dogs or chicken breasts, but please feel free to suggest what you think and why so that I can understand the logic. 2) Which post-fixative should I use and how long? 3) Are there any suggestions for the H&E staining procedure? Thanks! Komal _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sun Oct 23 07:18:12 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Oct 23 07:18:17 2011 Subject: [Histonet] Suggestions please... In-Reply-To: References: Message-ID: <74F1C549FBC24ACCA87265FDB643E61D@HP2010> Is there a slaughter house nearby? Call them, and have some documentation that you are from a university - such as a memo on a letterhead. Is there animal research at your university? Can they spare a rat? Try to do this right before class, so there is less autolysis. Put tissue in a plastic bag, and store in refrig for a few hours, until ready. Peggy Wenk, HTL(ASCP)SLS -----Original Message----- From: Komal Gada Sent: Saturday, October 22, 2011 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Suggestions please... Dear Histonetters, I'm currently teaching Histology at a University, and I was hoping for some suggestions on how to teach students to use a cryostat. I have several questions: 1) Since we do not have access to actual specimens, what would any of you recommend could be used as a viable option? So far, I'm thinking either hot dogs or chicken breasts, but please feel free to suggest what you think and why so that I can understand the logic. 2) Which post-fixative should I use and how long? 3) Are there any suggestions for the H&E staining procedure? Thanks! Komal _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Oct 23 09:40:13 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Oct 23 09:40:18 2011 Subject: [Histonet] test Message-ID: test Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org From lpwenk <@t> sbcglobal.net Sun Oct 23 18:04:34 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Oct 23 18:04:39 2011 Subject: [Histonet] cochineal mentioned in comic strip Message-ID: Just for fun: Check to see if your Sunday comic section carries Jump Start. Check out today?s 10/23/11 strip. Jump Start, a comic strip about a couple (policeman and nurse) and their kids, has the oldest girl wanting to be a cochineal insect for Halloween ? which is where histology gets carmine dye for the mucicarmine stain. http://www.gocomics.com/jumpstart/2011/10/23 If you want to read a fascinating book about the the role of carmine in the exploration of the America?s, enslaving the people of Central and South America, pirates stealing ships loaded with the dye, spying, politics, government and religion, and the ?unions? of the dyeing industry back then ? find or buy a copy of ?A Perfect Red: Empire, Espionage, and the Quest for the Color of Desire? by Amy Butler Greenfield, 2006. If you want a more abbreviated version, Anatech?s newsletter ?The Innovator? had an article about carmine in their Winter 2007 issue ? all about the history, and about why the quality of Mucicarmine has gone downhill in the past few years. (And also towards the end of the newsletter - what Anatech has done to try to improve the quality of the carmine. The article includes promoting their version of mucicarmine, so just a head?s up ? this is their newsletter to promote their products. But they do a great job at educating in general, too. So I enjoy reading and learning from their newsletters. Great photos of what stains SHOULD look like.) http://www.anatechltdusa.com/Innovators/Innovator12_06.pdf No ? I don?t get any money talking about the comic strip, the book or Anatech. I just think it?s neat to read about the history of dyes. And really great to to read about cochineal in a Sunday comic strip! Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 (The opinions expressed are my own.) From sadey <@t> hotmail.ca Mon Oct 24 06:00:14 2011 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Mon Oct 24 06:00:19 2011 Subject: [Histonet] Suggestions please... In-Reply-To: <74F1C549FBC24ACCA87265FDB643E61D@HP2010> References: , <74F1C549FBC24ACCA87265FDB643E61D@HP2010> Message-ID: You could buy some beef liver from the grocery store. Works great. > From: lpwenk@sbcglobal.net > To: kjgada@gmail.com; histonet@lists.utsouthwestern.edu > Date: Sun, 23 Oct 2011 08:18:12 -0400 > Subject: Re: [Histonet] Suggestions please... > CC: > > Is there a slaughter house nearby? Call them, and have some documentation > that you are from a university - such as a memo on a letterhead. > > Is there animal research at your university? Can they spare a rat? > > Try to do this right before class, so there is less autolysis. Put tissue in > a plastic bag, and store in refrig for a few hours, until ready. > > Peggy Wenk, HTL(ASCP)SLS > > -----Original Message----- > From: Komal Gada > Sent: Saturday, October 22, 2011 9:46 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Suggestions please... > > Dear Histonetters, > > I'm currently teaching Histology at a University, and I was hoping for some > suggestions on how to teach students to use a cryostat. I have several > questions: > > 1) Since we do not have access to actual specimens, what would any of you > recommend could be used as a viable option? > > So far, I'm thinking either hot dogs or chicken breasts, but please feel > free to suggest what you think and why so that I can understand the logic. > > 2) Which post-fixative should I use and how long? > > 3) Are there any suggestions for the H&E staining procedure? > > Thanks! > Komal > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joost.bruijntjes <@t> tno.triskelion.nl Mon Oct 24 06:15:24 2011 From: joost.bruijntjes <@t> tno.triskelion.nl (Bruijntjes, J.P. (Joost)) Date: Mon Oct 24 06:15:33 2011 Subject: [Histonet] (no subject) Message-ID: Hi all Is anyone of you familiar with the staining of CD4 cells in minipigs in formalin fixed and paraffin embedded tissues? Joost TNO.NL Joost Bruijntjes T +31 88 866 17 38 F +31 30 694 49 86 E joost.bruijntjes@tno.triskelion.nl Disclaimer From mamawooo <@t> hotmail.com Mon Oct 24 06:57:53 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Mon Oct 24 06:57:58 2011 Subject: [Histonet] cochineal mentioned in comic strip In-Reply-To: References: Message-ID: I concur Peggy, great book and great article. Jan Mahoney HT(ASCP) Omaha, NE > From: lpwenk@sbcglobal.net > To: Histonet@lists.utsouthwestern.edu > Date: Sun, 23 Oct 2011 19:04:34 -0400 > CC: > Subject: [Histonet] cochineal mentioned in comic strip > > Just for fun: Check to see if your Sunday comic section carries Jump Start. Check out today?s 10/23/11 strip. > > Jump Start, a comic strip about a couple (policeman and nurse) and their kids, has the oldest girl wanting to be a cochineal insect for Halloween ? which is where histology gets carmine dye for the mucicarmine stain. > http://www.gocomics.com/jumpstart/2011/10/23 > > If you want to read a fascinating book about the the role of carmine in the exploration of the America?s, enslaving the people of Central and South America, pirates stealing ships loaded with the dye, spying, politics, government and religion, and the ?unions? of the dyeing industry back then ? find or buy a copy of ?A Perfect Red: Empire, Espionage, and the Quest for the Color of Desire? by Amy Butler Greenfield, 2006. > > If you want a more abbreviated version, Anatech?s newsletter ?The Innovator? had an article about carmine in their Winter 2007 issue ? all about the history, and about why the quality of Mucicarmine has gone downhill in the past few years. (And also towards the end of the newsletter - what Anatech has done to try to improve the quality of the carmine. The article includes promoting their version of mucicarmine, so just a head?s up ? this is their newsletter to promote their products. But they do a great job at educating in general, too. So I enjoy reading and learning from their newsletters. Great photos of what stains SHOULD look like.) > http://www.anatechltdusa.com/Innovators/Innovator12_06.pdf > > No ? I don?t get any money talking about the comic strip, the book or Anatech. I just think it?s neat to read about the history of dyes. And really great to to read about cochineal in a Sunday comic strip! > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Hospital > Royal Oak, MI 48073 > > (The opinions expressed are my own.) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From darkdaym <@t> comcast.net Mon Oct 24 07:32:29 2011 From: darkdaym <@t> comcast.net (Mark Ray) Date: Mon Oct 24 07:32:37 2011 Subject: [Histonet] cochineal mentioned in comic strip In-Reply-To: References: Message-ID: <4EA55ADD.9010900@comcast.net> The history of logwood, the source of hematoxylin, was similar. People have always wanted colors and dyestuffs were very precious in the millennia before the advent of practical organic chemistry. It's also interesting to consider that Carmine made from Cochineal is a popular red food coloring. It's likely that we've all eaten this bug byproduct. On 10/23/2011 6:04 PM, Lee & Peggy Wenk wrote: > Just for fun: Check to see if your Sunday comic section carries Jump Start. Check out today?s 10/23/11 strip. > > Jump Start, a comic strip about a couple (policeman and nurse) and their kids, has the oldest girl wanting to be a cochineal insect for Halloween ? which is where histology gets carmine dye for the mucicarmine stain. > http://www.gocomics.com/jumpstart/2011/10/23 > > If you want to read a fascinating book about the the role of carmine in the exploration of the America?s, enslaving the people of Central and South America, pirates stealing ships loaded with the dye, spying, politics, government and religion, and the ?unions? of the dyeing industry back then ? find or buy a copy of ?A Perfect Red: Empire, Espionage, and the Quest for the Color of Desire? by Amy Butler Greenfield, 2006. > > If you want a more abbreviated version, Anatech?s newsletter ?The Innovator? had an article about carmine in their Winter 2007 issue ? all about the history, and about why the quality of Mucicarmine has gone downhill in the past few years. (And also towards the end of the newsletter - what Anatech has done to try to improve the quality of the carmine. The article includes promoting their version of mucicarmine, so just a head?s up ? this is their newsletter to promote their products. But they do a great job at educating in general, too. So I enjoy reading and learning from their newsletters. Great photos of what stains SHOULD look like.) > http://www.anatechltdusa.com/Innovators/Innovator12_06.pdf > > No ? I don?t get any money talking about the comic strip, the book or Anatech. I just think it?s neat to read about the history of dyes. And really great to to read about cochineal in a Sunday comic strip! > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Hospital > Royal Oak, MI 48073 > > (The opinions expressed are my own.) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From leiker <@t> buffalo.edu Mon Oct 24 08:08:18 2011 From: leiker <@t> buffalo.edu (Leiker, Merced) Date: Mon Oct 24 08:08:32 2011 Subject: [Histonet] cochineal mentioned in comic strip In-Reply-To: <4EA55ADD.9010900@comcast.net> References: <4EA55ADD.9010900@comcast.net> Message-ID: Thanks for forwarding these links. I found the comic strip and Anatech article fascinating (in their own rights). Regards, Merced -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Ray Sent: Monday, October 24, 2011 8:32 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cochineal mentioned in comic strip The history of logwood, the source of hematoxylin, was similar. People have always wanted colors and dyestuffs were very precious in the millennia before the advent of practical organic chemistry. It's also interesting to consider that Carmine made from Cochineal is a popular red food coloring. It's likely that we've all eaten this bug byproduct. On 10/23/2011 6:04 PM, Lee & Peggy Wenk wrote: > Just for fun: Check to see if your Sunday comic section carries Jump Start. Check out today?s 10/23/11 strip. > > Jump Start, a comic strip about a couple (policeman and nurse) and their kids, has the oldest girl wanting to be a cochineal insect for Halloween ? which is where histology gets carmine dye for the mucicarmine stain. > http://www.gocomics.com/jumpstart/2011/10/23 > > If you want to read a fascinating book about the the role of carmine in the exploration of the America?s, enslaving the people of Central and South America, pirates stealing ships loaded with the dye, spying, politics, government and religion, and the ?unions? of the dyeing industry back then ? find or buy a copy of ?A Perfect Red: Empire, Espionage, and the Quest for the Color of Desire? by Amy Butler Greenfield, 2006. > > If you want a more abbreviated version, Anatech?s newsletter ?The > Innovator? had an article about carmine in their Winter 2007 issue ? > all about the history, and about why the quality of Mucicarmine has > gone downhill in the past few years. (And also towards the end of the > newsletter - what Anatech has done to try to improve the quality of > the carmine. The article includes promoting their version of > mucicarmine, so just a head?s up ? this is their newsletter to promote > their products. But they do a great job at educating in general, too. > So I enjoy reading and learning from their newsletters. Great photos > of what stains SHOULD look like.) > http://www.anatechltdusa.com/Innovators/Innovator12_06.pdf > > No ? I don?t get any money talking about the comic strip, the book or Anatech. I just think it?s neat to read about the history of dyes. And really great to to read about cochineal in a Sunday comic strip! > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Hospital > Royal Oak, MI 48073 > > (The opinions expressed are my own.) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbrooks <@t> incytepathology.com Mon Oct 24 09:28:51 2011 From: mbrooks <@t> incytepathology.com (Matt Brooks) Date: Mon Oct 24 09:28:57 2011 Subject: [Histonet] Ventana's OptiView Detection Message-ID: <706224670091FE47997AEF88EFADE7CA01FA59C8@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Hello All, Is anyone using Ventana's OptiView detection kit? If so please let me know what you impression is. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbrooks@incytepathology.com 509-892-2744 (W) 720-253-7204 (C) From rennie1108 <@t> yahoo.com Mon Oct 24 10:39:06 2011 From: rennie1108 <@t> yahoo.com (Adrienne Anderson) Date: Mon Oct 24 10:39:11 2011 Subject: [Histonet] Used block cabinets Message-ID: <1319470746.7407.YahooMailNeo@web162113.mail.bf1.yahoo.com> Hello, I work for the Ohio Biorepository; we have about 1 1/2 million FFPE specimen in-house and are looking for better storage of our blocks. Does anyone know where I could buy reasonably priced cabinets, in large quantity (somewhere between 500-1000 cabinets)? ?Any and all advice would be appreciated. ?Thanks! Best, Adrienne Anderson From sbaldwin <@t> mhhcc.org Mon Oct 24 11:12:36 2011 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Mon Oct 24 11:12:38 2011 Subject: [Histonet] DISPOSAL OF SPECIMENS Message-ID: Hi histonetters what does everyone currently do with the vials the Pathologists empty when doing gross? Ours goes in biohazard bags, but someone mentioned to me they the labels should be taken off and then the vials put in the biohazard bag. If we did this we would just be taking off labels all day long. I have worked at 2 hospitals in my 35 years of experience and we have never done this. If we started this look at the clinical labs with blood specimens. Isn't there some sort of confidential agreement between the hospitals and hazard material companies? I would love to know your thoughts. Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. From plucas <@t> biopath.org Mon Oct 24 12:00:13 2011 From: plucas <@t> biopath.org (Paula Lucas) Date: Mon Oct 24 11:58:39 2011 Subject: [Histonet] Histotech Job Opening in Southern California Message-ID: A part-time entry level histotech position is available in our laboratory. Please email or fax resume to: Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 92708 plucas@biopath.org 714-755-2984 (fax) From Ashley.Troutman <@t> Vanderbilt.Edu Mon Oct 24 12:32:17 2011 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Mon Oct 24 12:32:25 2011 Subject: [Histonet] Ventana's OptiView Detection Message-ID: <7B310892042DA74CB3590053F424CFE61448AA5501@ITS-HCWNEM06.ds.Vanderbilt.edu> Hi Matt, I have worked a bit with this detection kit and my impression is that is extremely sensitive and should be able to boost signal considerably. I have also been able to shave off an average of 30 minutes off several stains. (Cyclin D1, which is one of our longer stains at 4 hours is down to 2.5) You can further enhance the stain (or shorten the time) with the amp kit, but I did not find it necessary. I thought it was particularly good on nuclear stains. There is also a good deal of flexibility with the kit and you should be able to get a great stain in less time. Hope that was helpful. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Content-Type: text/plain; charset="us-ascii" Hello All, Is anyone using Ventana's OptiView detection kit? If so please let me know what you impression is. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology From LSebree <@t> uwhealth.org Mon Oct 24 14:38:51 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Oct 24 14:38:55 2011 Subject: [Histonet] Seeking Beta-Amyloid not needing FA pretreatment Message-ID: Hi everyone, I asked this question a long time ago but would like to see if anything new is available. We'd like to totally automate our Beta-Amyloid antibody but in order to do so, we need an antibody that does not require Formic Acid pretreatment. Anyone (especially vendors) no of any on the market? Thanks, Linda From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Oct 24 14:50:07 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Oct 24 14:50:41 2011 Subject: [Histonet] RE: Seeking Beta-Amyloid not needing FA pretreatment In-Reply-To: References: Message-ID: We use Dako's Beta Amyloid with high pH heat retrieval. Works very well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A [LSebree@uwhealth.org] Sent: Monday, October 24, 2011 3:38 PM To: Histonet@lists.utsouthwestern.edu Cc: Normington Lacy Subject: [Histonet] Seeking Beta-Amyloid not needing FA pretreatment Hi everyone, I asked this question a long time ago but would like to see if anything new is available. We'd like to totally automate our Beta-Amyloid antibody but in order to do so, we need an antibody that does not require Formic Acid pretreatment. Anyone (especially vendors) no of any on the market? Thanks, Linda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Mon Oct 24 15:25:49 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Mon Oct 24 15:26:13 2011 Subject: [Histonet] RE: Seeking Beta-Amyloid not needing FA pretreatment In-Reply-To: References: Message-ID: We have success with 6E10 and 4G8 clones using citrate retrieval. Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Monday, October 24, 2011 3:39 PM To: Histonet@lists.utsouthwestern.edu Cc: Normington Lacy Subject: [Histonet] Seeking Beta-Amyloid not needing FA pretreatment Hi everyone, I asked this question a long time ago but would like to see if anything new is available. We'd like to totally automate our Beta-Amyloid antibody but in order to do so, we need an antibody that does not require Formic Acid pretreatment. Anyone (especially vendors) no of any on the market? Thanks, Linda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From daniela.bodemer <@t> mcri.edu.au Mon Oct 24 17:57:05 2011 From: daniela.bodemer <@t> mcri.edu.au (Daniela Bodemer) Date: Mon Oct 24 17:57:13 2011 Subject: [Histonet] processor stopped - help! Message-ID: <9DF797D618351549B984596F01A1FE1D01F6DA18@murmx.mcri.edu.au> Hi all, When I arrived the lab this morning I found the processor basket with my cassettes stuck between the two paraffin tanks. I suppose it went through the first cycle but not the second one, so its missing 1.5 hrs of paraffin infusion. Should I put them back in hot paraffin for 1.5 hours or what can I do to save the samples? Thanks for your advice, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03 9345 4116) E daniela.bodemer@mcri.edu.au www.mcri.edu.au This e-mail and any attachments to it (the "Communication") are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From baderbo <@t> gmail.com Mon Oct 24 18:10:37 2011 From: baderbo <@t> gmail.com (Bader Siddiki) Date: Mon Oct 24 18:10:41 2011 Subject: [Histonet] We need someone to test two antibodies. Message-ID: Hello Histonetters We are in need of someone who can test two antibodies: CD71 and CD74 for us. --IHC Frozen Tissue (Methanol fixed tonsil) for both antibodies. --ImmunoFluorescence - Frozen tissue (Methanol fixed) We have FITC conjugated antibodies. Please contact us at : BaderBO@Gmail.com If you need more information, please visit our web site: www.ImmunoBioScience.com and look for monoclonal antibodies catalog # MM-1027 and MM-1029 Thank you. Bader -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Gr??en / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten ?????? Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience Corp. (IBSC) Phone: + 1 425 367 4601 Fax: + 1 425 367 4817 cell (mobile) phone: + 1 425 314 0199 e-mail address: BaderBo@Gmail.com Web site: www.ImmunoBioScience.Com Marketing: phone: + 1 650 343 IBSC (4272) E-mail: AnitaIBSC@Aol.com From chapcl <@t> yahoo.com Mon Oct 24 18:12:19 2011 From: chapcl <@t> yahoo.com (William Chappell) Date: Mon Oct 24 18:12:23 2011 Subject: [Histonet] processor stopped - help! In-Reply-To: <9DF797D618351549B984596F01A1FE1D01F6DA18@murmx.mcri.edu.au> References: <9DF797D618351549B984596F01A1FE1D01F6DA18@murmx.mcri.edu.au> Message-ID: Yes, nothing to worry about here (I am assuming an autotechnicon?). The first paraffin would have dried and sealed the blocks keeping the tissue just fine, however you are probably right in thinking they did not get enough paraffin infiltration. Try another hour in the second paraffin. If you have the time, the full 1 1/2 hours is best. Will On Oct 24, 2011, at 3:57 PM, Daniela Bodemer wrote: > Hi all, > > > > When I arrived the lab this morning I found the processor basket with my > cassettes stuck between the two paraffin tanks. I suppose it went > through the first cycle but not the second one, so its missing 1.5 hrs > of paraffin infusion. Should I put them back in hot paraffin for 1.5 > hours or what can I do to save the samples? > > > > Thanks for your advice, > > > > Daniela Bodemer > > Research Assistant > > Surgical Research, Infection and Immunity > > > > Murdoch Childrens Research Institute > > The Royal Children's Hospital > > Flemington Road Parkville Victoria 3052 Australia > > T 03 9345 5930 T (03 9345 4116) > > E daniela.bodemer@mcri.edu.au > > www.mcri.edu.au > > > > This e-mail and any attachments to it (the "Communication") are, unless > otherwise stated, confidential, may contain copyright material and is > for the use only of the intended recipient. If you receive the > Communication in error, please notify the sender immediately by return > e-mail, delete the Communication and the return e-mail, and do not read, > copy, retransmit or otherwise deal with it. Any views expressed in the > Communication are those of the individual sender only, unless expressly > stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 > 006 566 972 or any of its related entities. MCRI does not accept > liability in connection with the integrity of or errors in the > Communication, computer virus, data corruption, interference or delay > arising from or in respect of the Communication. > > P Please consider the environment before printing this email > > > > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From daniela.bodemer <@t> mcri.edu.au Mon Oct 24 19:48:59 2011 From: daniela.bodemer <@t> mcri.edu.au (Daniela Bodemer) Date: Mon Oct 24 19:49:09 2011 Subject: [Histonet] processor stopped - help! Message-ID: <9DF797D618351549B984596F01A1FE1D01F6DAC9@murmx.mcri.edu.au> I am glad to hear the samples will be fine. Thanks everyone! Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03 9345 4116) E daniela.bodemer@mcri.edu.au www.mcri.edu.au This e-mail and any attachments to it (the "Communication") are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From JWeems <@t> sjha.org Mon Oct 24 21:02:45 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Oct 24 21:03:08 2011 Subject: [Histonet] RE: processor stopped - help! In-Reply-To: <9DF797D618351549B984596F01A1FE1D01F6DA18@murmx.mcri.edu.au> References: <9DF797D618351549B984596F01A1FE1D01F6DA18@murmx.mcri.edu.au> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408307FCFB2@CHEXCMS10.one.ads.che.org> Yes, that should work fine. Best! j ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniela Bodemer [daniela.bodemer@mcri.edu.au] Sent: Monday, October 24, 2011 6:57 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] processor stopped - help! Hi all, When I arrived the lab this morning I found the processor basket with my cassettes stuck between the two paraffin tanks. I suppose it went through the first cycle but not the second one, so its missing 1.5 hrs of paraffin infusion. Should I put them back in hot paraffin for 1.5 hours or what can I do to save the samples? Thanks for your advice, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03 9345 4116) E daniela.bodemer@mcri.edu.au www.mcri.edu.au This e-mail and any attachments to it (the "Communication") are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From gonavy2003 <@t> gmail.com Mon Oct 24 23:00:16 2011 From: gonavy2003 <@t> gmail.com (Rick T.) Date: Mon Oct 24 23:00:24 2011 Subject: [Histonet] Suggestions please... Message-ID: <000c01cc92ca$9c5420f0$d4fc62d0$@gmail.com> Komal - I once found a lymph node in a roast from the market. A quick "home version" fixative with vinegar and isopropyl kept it good until I could get it into formalin the next day, it cut and demonstrated great. If by chance there is a university with an agriculture program, that could be a source of tissue also, and autolysis could be minimized. Rick T. From tmclaugh <@t> NEMOURS.ORG Tue Oct 25 07:25:25 2011 From: tmclaugh <@t> NEMOURS.ORG (McLaughlin, Terry ) Date: Tue Oct 25 07:26:00 2011 Subject: [Histonet] Help finding metal embedding molds Message-ID: <0B0238D44CE7C04E9D32455C8643D893E3FBEB@wlmmsx02.nemours.org> Hello All, This is my first time posting a request. We use metal embedding molds, and would like to order one particular size. The outer measurements are 2.5mm x 2.5mm x 0.6 mm. We are wondering if anyone has approximately 40-50 of them we could purchase-new or used. The reason we need this size is because most of our research tissue fits perfectly into this size and depth. We have 18 of them and quite often need 30-60 for a particular project. We found them from one of the vendors, but the price is quite expensive. We are hoping someone out there can help us. Much Thanks, Terry McLaughlin Histology Specialist AI DuPont Hospital for Children 1600 Rockland Rd. Wilmington, DE. 19803 phone 302-651-6771 fax-302-651-5010 From Bryan.Watson <@t> neiurology.com Tue Oct 25 08:26:02 2011 From: Bryan.Watson <@t> neiurology.com (Bryan Watson) Date: Tue Oct 25 08:26:08 2011 Subject: [Histonet] Urine cytologies Message-ID: <1C03BD76DC9BD14EA266F05C65D173B474CDA26D60@NEIU-SBS.Niu.lan> What is a good reagent for lysing red cells in extremely bloody urine specimens for cytology? thanks, Bryan From thiggins <@t> cddmedical.com Tue Oct 25 08:28:04 2011 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Tue Oct 25 08:28:12 2011 Subject: [Histonet] DISPOSAL OF SPECIMENS References: <20111024170731.DC5F2131284D@barracuda.crvinc.net> Message-ID: <002501cc9319$eddcd580$e001a8c0@cdd.loc> I have been in the histology field for 20 years and never heard of having to remove the labels prior to disposal through an approved vendor. I don't believe that the labels from blood tubes are removed prior to disposal, this would be the same type of scenario. Thanks, Tim Message: 9 Date: Mon, 24 Oct 2011 12:36 -0400 From: "Sara Baldwin/mhhcc.org" Subject: [Histonet] DISPOSAL OF SPECIMENS To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi histonetters what does everyone currently do with the vials the Pathologists empty when doing gross? Ours goes in biohazard bags, but someone mentioned to me they the labels should be taken off and then the vials put in the biohazard bag. If we did this we would just be taking off labels all day long. I have worked at 2 hospitals in my 35 years of experience and we have never done this. If we started this look at the clinical labs with blood specimens. Isn't there some sort of confidential agreement between the hospitals and hazard material companies? I would love to know your thoughts. Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. From DKBoyd <@t> chs.net Tue Oct 25 08:46:06 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Oct 25 08:46:33 2011 Subject: [Histonet] Urine cytologies In-Reply-To: <1C03BD76DC9BD14EA266F05C65D173B474CDA26D60@NEIU-SBS.Niu.lan> Message-ID: Saponin and Calcium Gluconate. Let me know if you would like the procedure and I will send it to you under separate cover. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Bryan Watson Sent by: histonet-bounces@lists.utsouthwestern.edu 10/25/2011 09:26 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Urine cytologies What is a good reagent for lysing red cells in extremely bloody urine specimens for cytology? thanks, Bryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From thiggins <@t> cddmedical.com Tue Oct 25 09:48:46 2011 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Tue Oct 25 09:49:00 2011 Subject: [Histonet] p57 antibody Message-ID: <004701cc9325$36ed82f0$e001a8c0@cdd.loc> Hey Histonetters, Does anyone use the p57 (kip 2) antibody from Thermo (Lab Vision)? I am still having trouble with the pathologist saying it's not staining the syacitiotrophoblasts. Any help with a protocol or even me being able to send in a slide that you could stain and send back to be would ideal. Any other help would also be greatly appreciated. Thanks, Tim From JWeems <@t> sjha.org Tue Oct 25 11:16:29 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Oct 25 11:16:38 2011 Subject: [Histonet] FW: CoC standards #2 Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408306E6C49@CHEXCMS10.one.ads.che.org> I forgot and sent with the standards attached... so now I'm sending without them... TIA! j Hey Folks, Are any of you are involved with Cancer Program Standards and the review necessary to document compliance with the cancer protocols? Standard 2.2 addresses the review of cases to confirm that the protocols are reviewed for required data points - a minimum of 10% of eligible cases or a maximum of 300 cases. Would you share with me how you handle this in your facility? Many thanks, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From irena.kirbis <@t> hotmail.com Tue Oct 25 11:55:03 2011 From: irena.kirbis <@t> hotmail.com (IRENA SREBOTNIK KIRBIS) Date: Tue Oct 25 11:55:10 2011 Subject: [Histonet] Urine cytologies In-Reply-To: References: <1C03BD76DC9BD14EA266F05C65D173B474CDA26D60@NEIU-SBS.Niu.lan>, Message-ID: we prefer filtration of urines through 5 mikron policarbonate filters Irena Kirbis Ljubljana, Slovenia > To: Bryan.Watson@neiurology.com > From: DKBoyd@chs.net > Date: Tue, 25 Oct 2011 09:46:06 -0400 > Subject: Re: [Histonet] Urine cytologies > CC: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > Saponin and Calcium Gluconate. Let me know if you would like the > procedure and I will send it to you under separate cover. > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: > 804-765-5582 l dkboyd@chs.net > > > > > > > > Bryan Watson > Sent by: histonet-bounces@lists.utsouthwestern.edu > 10/25/2011 09:26 AM > > To > "histonet@lists.utsouthwestern.edu" > cc > > Subject > [Histonet] Urine cytologies > > > > > > > What is a good reagent for lysing red cells in extremely bloody urine > specimens for cytology? > thanks, > Bryan > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jpgrock37 <@t> yahoo.com Tue Oct 25 12:21:25 2011 From: jpgrock37 <@t> yahoo.com (Jessica Piche) Date: Tue Oct 25 12:21:29 2011 Subject: [Histonet] Histostar Embedding Station Message-ID: <1319563285.61798.YahooMailNeo@web121504.mail.ne1.yahoo.com> Hi Everyone, ? Just curious if anyone is using the Histostar Embedding Station? And I am curious to know how you like/dislike it? We have one? and have been using it for about 4 weeks and are already needing parts. Thanks, ? Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital From kreif <@t> stegh.on.ca Tue Oct 25 12:52:28 2011 From: kreif <@t> stegh.on.ca (Kevin Reif) Date: Tue Oct 25 12:52:42 2011 Subject: [Histonet] Hi Group: Message-ID: <4EA6BF1D.2151.00B6.0@stegh.on.ca> Hi Group: Looking for opinions on cassette and slide labellers?? If you are using one please let me know the make, model and what you think of the machine, good or bad. Thanks in advance for all your input. Kevin Reif Senior Technologist - Pathology St. Thomas-Elgin General Hospital St. Thomas, On. Can. __________________________________________________________________________ St. Thomas Elgin General Hospital 189 Elm St. St. Thomas Ontario N5R 5C4 (519) 631-2020 http://www.stegh.on.ca Proud recipients of 2010 OHA Quality Healthcare Workplace Award - Platinum __________________________________________________________________________ Confidentiality/Privacy Notice: This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. For more information on our Privacy Policy, please see our website at http://www.stegh.on.ca From swongman <@t> yahoo.com Tue Oct 25 12:58:04 2011 From: swongman <@t> yahoo.com (Steve Wong) Date: Tue Oct 25 12:58:07 2011 Subject: [Histonet] Please unsubscribe Message-ID: <1319565484.85941.YahooMailClassic@web65902.mail.ac4.yahoo.com> Hello, Please unsubscribe me from this list. swongman@yahoo.com Thanks! From Sandra.Harrison3 <@t> va.gov Tue Oct 25 12:59:41 2011 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Tue Oct 25 13:00:05 2011 Subject: [Histonet] saving slides between levels for IHC? Message-ID: Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 From badzrosari <@t> yahoo.com Tue Oct 25 13:02:10 2011 From: badzrosari <@t> yahoo.com (Badz Yahoo) Date: Tue Oct 25 13:03:37 2011 Subject: [Histonet] EMS 9000 Message-ID: <80FD49AB-B170-4680-B36E-EEECD98F2800@yahoo.com> Hi histonetts..Anyone using the microwave machine EMS 9000?I appreciate if someone can give me some feedback..tanx From Timothy.Morken <@t> ucsfmedctr.org Tue Oct 25 13:05:10 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Oct 25 13:05:24 2011 Subject: [Histonet] RE: saving slides between levels for IHC? In-Reply-To: References: Message-ID: <8D7C2D242DBD45498006B21122072BF89448A30D@MCINFRWEM003.ucsfmedicalcenter.org> Sandy, we have various protocols, some that have unstained between those to be stained. However, we have moved away from having "extras" cut "just in case" because we had thousands of unstained slides stored that were never used. After a few months they may be unreliable for staining. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Tuesday, October 25, 2011 11:00 AM To: Webb, Dorothy L; Horge, Pamela J; rollmann.denis@mayo.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] saving slides between levels for IHC? Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Tue Oct 25 14:15:23 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Oct 25 14:15:30 2011 Subject: [Histonet] saving slides between levels for IHC? In-Reply-To: References: Message-ID: <4EA6D28B.2B7F.00C9.1@geisinger.edu> we do for certain part types. sentinel lymph nodes for melanoma, renal bxs, liver core bxs, prostate needle core bxs. >>> "Harrison, Sandra C." 10/25/2011 1:59 PM >>> Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From rlhenshall_powell <@t> yahoo.co.uk Tue Oct 25 15:44:11 2011 From: rlhenshall_powell <@t> yahoo.co.uk (Rhonda Henshall-Powell) Date: Tue Oct 25 15:44:15 2011 Subject: [Histonet] Seeking Field Application Specialists and Education Coordinator for CA-based Cancer Diagnostics Company Message-ID: <1319575451.47919.YahooMailNeo@web29501.mail.ird.yahoo.com> Hello Histonetters! ? Here I am again -?An industry-leading IHC/ISH diagnostics company is seeking an in-house Education Coordinator and two Field Application Specialists (Midwest and Northwest) requiring significant travel: Field Applications Specialist Provide expert level technical support knowledge of Anatomic Pathology principles to troubleshoot and support instrumentation, software, and train lab personnel on use, optimisation and validation of IHC and ISH reagents. If interested or know of someone who may be interested, please reply directly to me (rlhenshall_powell@yahoo.co.uk) for more information. ? Thank you ? Best Regards, Rhonda From foreightl <@t> gmail.com Tue Oct 25 17:09:32 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Oct 25 17:09:36 2011 Subject: [Histonet] saving slides between levels for IHC? In-Reply-To: <4EA6D28B.2B7F.00C9.1@geisinger.edu> References: <4EA6D28B.2B7F.00C9.1@geisinger.edu> Message-ID: We tend to take extras between levels especially on scant or needle biopsy tissue. We do waste several thousand slides per year, but by doing this it allows us to give the pathologist several representative sections and also save tissue for future IHC and special stains. On Tue, Oct 25, 2011 at 12:15 PM, Angela Bitting wrote: > we do for certain part types. sentinel lymph nodes for melanoma, renal bxs, > liver core bxs, prostate needle core bxs. > > >>> "Harrison, Sandra C." 10/25/2011 1:59 PM >>> > Does anyone know if it is standard to save sections from between the > levels for IP? Is it standard at your institution? > > > > > > Sandy C. Harrison, HTL (ASCP) > > Histology Supervisor > > Minneapolis VA > > 612-467-2449 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. It is > intended solely for the addressee. Access to this message by anyone else is > unauthorized. If you are not the intended recipient, any disclosure, > copying, distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you have received this > message in error, please delete all electronic copies of this message (and > the documents attached to it, if any), destroy any hard copies you may have > created and notify me immediately by replying to this email. Thank you. > > Geisinger Health System utilizes an encryption process to safeguard > Protected Health Information and other confidential data contained in > external e-mail messages. If email is encrypted, the recipient will receive > an e-mail instructing them to sign on to the Geisinger Health System Secure > E-mail Message Center to retrieve the encrypted e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From carl.hobbs <@t> kcl.ac.uk Wed Oct 26 01:51:56 2011 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Wed Oct 26 01:53:37 2011 Subject: [Histonet] Re: Seeking Beta-Amyloid not needing FA pretreatment Message-ID: <11D9615B89C10747B1C985966A63D7CA3826477A48@KCL-MAIL04.kclad.ds.kcl.ac.uk> Be careful: HIER may expose not only extracellular amyloid plaques but also intracellular APP ( or...is it intracellular A beta ???? ;-)) Eg: with 6E10.... it is directed against an epitope found in APP and also A beta: if you do Formic acid, only extracellular plaques are positive...after HIER, there is also intracellular positivity. In my experience. Some examples of this can be found here, in the image gallery : http://www.immunoportal.com/ Whichever way you go....good luck. Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 From sfonner <@t> labpath.com Wed Oct 26 06:28:54 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Wed Oct 26 06:33:53 2011 Subject: [Histonet] saving slides between levels for IHC? In-Reply-To: References: Message-ID: <000001cc93d2$71a7f670$54f7e350$@com> Sandy, I don't think it is a "standard" procedure. However, our pathologist will frequently ask us to keep intervening levels for possible IHC on certain cases that are small or where the tumor is about to be exhausted. We are a dermatopathology lab. When it is requested, we cut the whole case on a clean waterbath, using distilled water and no additives, on plus slides. The routine H&E's are stained normally and the other slides are dried and held for possible immunos. Sheila KDL Pathology Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Tuesday, October 25, 2011 2:00 PM To: Webb, Dorothy L; Horge, Pamela J; rollmann.denis@mayo.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] saving slides between levels for IHC? Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Wed Oct 26 08:42:18 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Oct 26 08:42:25 2011 Subject: [Histonet] RE: Re: Seeking Beta-Amyloid not needing FA pretreatment In-Reply-To: <11D9615B89C10747B1C985966A63D7CA3826477A48@KCL-MAIL04.kclad.ds.kcl.ac.uk> References: <11D9615B89C10747B1C985966A63D7CA3826477A48@KCL-MAIL04.kclad.ds.kcl.ac.uk> Message-ID: Interesting....we see APP on frozen sections with 6E10, but not in paraffin sections after citrate HIER. We use the monoclonal from Covance, 1:12000 overnight at 4C. Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl Sent: Wednesday, October 26, 2011 2:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Seeking Beta-Amyloid not needing FA pretreatment Be careful: HIER may expose not only extracellular amyloid plaques but also intracellular APP ( or...is it intracellular A beta ???? ;-)) Eg: with 6E10.... it is directed against an epitope found in APP and also A beta: if you do Formic acid, only extracellular plaques are positive...after HIER, there is also intracellular positivity. In my experience. Some examples of this can be found here, in the image gallery : http://www.immunoportal.com/ Whichever way you go....good luck. Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From rbrown <@t> mst.edu Wed Oct 26 08:49:26 2011 From: rbrown <@t> mst.edu (Roger Brown) Date: Wed Oct 26 08:49:35 2011 Subject: [Histonet] Please remove my name Message-ID: Hello, Please remove my name from the Histonet mailing list. Thank you, Roger Brown (rbrown@mst.edu) From vjp2105 <@t> columbia.edu Wed Oct 26 08:56:15 2011 From: vjp2105 <@t> columbia.edu (Vanessa J. Phelan) Date: Wed Oct 26 08:56:20 2011 Subject: [Histonet] Please remove my name Message-ID: Hi, please remove my name from the mailing list. Thank you From BDeBrosse-Serra <@t> isisph.com Wed Oct 26 09:32:57 2011 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Wed Oct 26 09:33:06 2011 Subject: [Histonet] RE: Please remove my name In-Reply-To: References: Message-ID: <493CAA64F203E14E8823737B9EE0E25F091E91273E@EXCHMB01.isis.local> If you want to be removed from the list, you have to do it yourself by going to the website below. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Brown Sent: Wednesday, October 26, 2011 6:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Please remove my name Hello, Please remove my name from the Histonet mailing list. Thank you, Roger Brown (rbrown@mst.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Wed Oct 26 10:10:43 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Oct 26 10:10:49 2011 Subject: [Histonet] saving slides between levels for IHC? In-Reply-To: <000001cc93d2$71a7f670$54f7e350$@com> References: <000001cc93d2$71a7f670$54f7e350$@com> Message-ID: <1319641843.94105.YahooMailNeo@web112302.mail.gq1.yahoo.com> I think it depends on what the specimen is. All of the places Ive worked have always kept extras(levels) on sentinal nodes for keratins, also for prostate needle biopsies for p63/504s or other IHC's. ? Really doesnt it come down to what the Pathologist needs to make a diagnosis for the patient? ? I know it's a pain to cut so many levels and save them. Its also costly. But trust me, none of that is as bad as the sinking feeling you get when a biopsy has been exhausted and they need more stains :( ? Hope this helps. Just my 2 cents worth....... ? Kim Donadio ________________________________ From: Sheila Fonner To: "'Harrison, Sandra C.'" ; histonet@lists.utsouthwestern.edu Sent: Wednesday, October 26, 2011 7:28 AM Subject: RE: [Histonet] saving slides between levels for IHC? Sandy, I don't think it is a "standard" procedure.? However, our pathologist will frequently ask us to keep intervening levels for possible IHC on certain cases that are small or where the tumor is about to be exhausted.? We are a dermatopathology lab.? When it is requested, we cut the whole case on a clean waterbath, using distilled water and no additives, on plus slides. The routine H&E's are stained normally and the other slides are dried and held for possible immunos. Sheila KDL Pathology Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Tuesday, October 25, 2011 2:00 PM To: Webb, Dorothy L; Horge, Pamela J; rollmann.denis@mayo.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] saving slides between levels for IHC? Does anyone know if it is standard? to save sections from between the levels for IP?? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfonner <@t> labpath.com Wed Oct 26 11:00:59 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Wed Oct 26 11:05:52 2011 Subject: [Histonet] EBER ISH Message-ID: <001901cc93f8$74289190$5c79b4b0$@com> Histonetters, Is anyone out there using the EBER ISH probes from Ventana on the Ultra Platform? I have a few questions. Did you need to get new software installed? Was there any training involved? And do you have successful protocols that you are willing to share? I have asked the questions before, but at the time there was nobody actually using it. I know that there were inquiries about the protocols because of the ASR status and the fact that Ventana cannot give you any advice. Any and all information would be greatly appreciated since we are wanting to start it up at our facility. Thanks, Sheila KDL Pathology Knoxville, TN From sfonner <@t> labpath.com Wed Oct 26 11:04:28 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Wed Oct 26 11:09:22 2011 Subject: [Histonet] Antibody questions Message-ID: <001e01cc93f8$f085e120$d191a360$@com> Is there anyone who is doing myc, Bap1, Pten, or CD123 for dermpath specimens, and who is your vendor? Do you like the products, and what platform are you using? Thanks for any information you may have. These are new requests from our pathologist. Sheila From rgsremoval <@t> yahoo.com Wed Oct 26 12:04:23 2011 From: rgsremoval <@t> yahoo.com (Raymond Stone) Date: Wed Oct 26 12:04:27 2011 Subject: [Histonet] removal me from histo net Message-ID: <1319648663.22485.YahooMailClassic@web125315.mail.ne1.yahoo.com> Please removal me for histo net From MSHERWOOD <@t> PARTNERS.ORG Wed Oct 26 13:27:31 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Wed Oct 26 13:27:36 2011 Subject: [Histonet] removal me from histo net In-Reply-To: <1319648663.22485.YahooMailClassic@web125315.mail.ne1.yahoo.com> References: <1319648663.22485.YahooMailClassic@web125315.mail.ne1.yahoo.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5B18@PHSXMB30.partners.org> To all who want to be removed from the Histonet List--you have to do it yourself! Go to the website and follow the directions. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Raymond Stone Sent: Wednesday, October 26, 2011 1:04 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] removal me from histo net Please removal me for histo net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From thiggins <@t> cddmedical.com Wed Oct 26 14:14:33 2011 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Wed Oct 26 14:14:40 2011 Subject: [Histonet] Re: Histostar Embedding Station References: <20111026170726.01AD31541AF7@barracuda.crvinc.net> Message-ID: <013b01cc9413$7f134300$e001a8c0@cdd.loc> Hey Jessica, We have two Histostar's that have been here since March and have not had any issues with them. Can't say anything bad about them, yet. Thanks, Tim Subject: [Histonet] Histostar Embedding Station To: "Histonet@lists.utsouthwestern.edu" Message-ID: <1319563285.61798.YahooMailNeo@web121504.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Everyone, Just curious if anyone is using the Histostar Embedding Station? And I am curious to know how you like/dislike it? We have one and have been using it for about 4 weeks and are already needing parts. Thanks, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital From olivepitt <@t> rocketmail.com Wed Oct 26 14:22:22 2011 From: olivepitt <@t> rocketmail.com (Olive Pitt) Date: Wed Oct 26 14:22:26 2011 Subject: [Histonet] B220 and F4/80 on Ventana Discovery Message-ID: <1319656942.77550.YahooMailNeo@web125302.mail.ne1.yahoo.com> Hello, ? Is anyone using B220 (RA3-6B2) or F4/80 (C1:A3-1) on the Ventana Discovery? Any protocols or tips would be greatly appreciated.? I am new to using Ventanas and would like to use these abs on a Discovery instead of a Dako.? Also, do all antibodies that work on Dako eventually work on the Ventana?? I know it is a closed system, so im not sure if everything eventually will work. ? thank you for any input -Olive From YuJ2 <@t> upmc.edu Wed Oct 26 14:44:23 2011 From: YuJ2 <@t> upmc.edu (Yu, Jian) Date: Wed Oct 26 14:44:28 2011 Subject: [Histonet] Abs for detecting immune cells in mouse FFPE sections In-Reply-To: <1319656942.77550.YahooMailNeo@web125302.mail.ne1.yahoo.com> References: <1319656942.77550.YahooMailNeo@web125302.mail.ne1.yahoo.com> Message-ID: Dear all, It will be greatly appreciated if you can share experience/information on the Abs against immune cells (vendor and cat#) that work for IHC or IF using mouse paraffin sections. Info on alternative Abs for these populations can be helpful too. I have a small research lab with limited budget for buying new Abs. Gr-1 (granulocytes) B220 (B cells) CD3 (T cells) Thank you! Jian Yu, Ph.D. University of Pittsburgh From shive003 <@t> umn.edu Wed Oct 26 15:00:13 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Oct 26 15:00:19 2011 Subject: [Histonet] Abs for detecting immune cells in mouse FFPE sections Message-ID: > CD3 - rabbit polyclonal Ab from ThermoScientific (cat. # RB-360) works on > mouse FFPE tissue. > > Jan Shivers > Senior Scientist > Histology/IHC/EM Section Head > Pathology Teaching Program > University of Minnesota > Veterinary Diagnostic Laboratory > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > > (Confidentiality Notice: This message, together with any attachments, is > intended only for the use of the individual or entity to which it is > addressed and may contain confidential or privileged information. If you > think you have received this message in error, please advise the sender > and then delete this message and any attachments immediately.) > > ----- Original Message ----- > From: "Yu, Jian" > To: > Sent: Wednesday, October 26, 2011 2:44 PM > Subject: [Histonet] Abs for detecting immune cells in mouse FFPE sections > > > Dear all, > > It will be greatly appreciated if you can share experience/information on > the Abs against immune cells (vendor and cat#) that work for IHC or IF > using mouse paraffin sections. Info on alternative Abs for these > populations can be helpful too. I have a small research lab with limited > budget for buying new Abs. > > Gr-1 (granulocytes) > B220 (B cells) > CD3 (T cells) > > Thank you! > > Jian Yu, Ph.D. > University of Pittsburgh > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AGleiberman <@t> cbiolabs.com Wed Oct 26 15:08:17 2011 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Wed Oct 26 15:08:24 2011 Subject: [Histonet] RE: Abs for detecting immune cells in mouse FFPE sections In-Reply-To: References: <1319656942.77550.YahooMailNeo@web125302.mail.ne1.yahoo.com> Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C3425BC45@cbiolabs05.CBiolabs.local> CD3 Rabbit monoclonal from Fisher (MA1-39552) works fine in IF on mouse paraffin sections, B220 rat monoclonal from eBioscience (14-0452-85) works fine in IF on mouse paraffin sections. Used both in 1:100 dilution. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yu, Jian Sent: Wednesday, October 26, 2011 3:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Abs for detecting immune cells in mouse FFPE sections Dear all, It will be greatly appreciated if you can share experience/information on the Abs against immune cells (vendor and cat#) that work for IHC or IF using mouse paraffin sections. Info on alternative Abs for these populations can be helpful too. I have a small research lab with limited budget for buying new Abs. Gr-1 (granulocytes) B220 (B cells) CD3 (T cells) Thank you! Jian Yu, Ph.D. University of Pittsburgh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From melissa <@t> alliedsearchpartners.com Wed Oct 26 15:32:40 2011 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Wed Oct 26 15:32:51 2011 Subject: [Histonet] Histotech Needed in Naples, FL for Day Shift-M-F (Permanent)-HOT JOB Message-ID: Allied Search Partners is currently looking for a qualified histology professional for a full time/permanent position. Location: Naples, FL Schedule: Full Time, 6am-2:30 pm, Monday-Friday. Work one weekend per month. Every 3rd weekend. Requirements: 2 years of formal education beyond HS OR and Associates Degree Florida License in Histology or Clinical Laboratory License Summary: Responsible for the preparation of tissue specimens for microscopic examination by our Pathologist. Tissue processing, embedding, cutting, staining, and performing other special procedures such as frozen, cytology preparation, and bone marrow collection. To apply: Please send resume to melissa@alliedsearchpartners.com Benefits: Medical and Dental Insurance ? Your coverage will begin the first day of the month following your 90 day probationary period Competitive Salaries and Shift Differentials Free Basic Life Insurance Accidental Death/Dismemberment Insurance Vision Care Flexible Spending Accounts A generous Paid Time Off Program - up to 26 days per year Extended Illness Bank 401 K Plan ? matching contributions on the first 4% of eligible pay after the 1st year - 50% vested after 2 years and 100% after 3 years Holiday Pay Staffing Incentives Short Term Disability Free Long Term Disability Universal Life Insurance Tax-sheltered Annuities Employee Referral Bonus 2 Wellness Centers - Fitness Clubs Lunch Payroll Deduction Program - discounts Free Onsite Parking Team Share/Performance Bonus PTO Cashout Prepaid Legal Services College Savings Plan At Work Weight Watchers Program Annual Service Awards Banquet and Gifts Monthly Service Recognition Superstar Program - Reward and Recognition for Patient Satisfaction Silent Heroes Recognition Key Contributor Program ($1000 bonus) -- Melissa Phelan, President Laboratory Staffing Allied Search Partners http://www.linkedin.com/in/melissaphelan P: 888-388-7571 F: 888-388-7572 C: 407-697-1175 www.alliedsearchpartners.com From PAMarcum <@t> uams.edu Wed Oct 26 16:03:46 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Oct 26 16:04:08 2011 Subject: [Histonet] Re: Histostar Embedding Station In-Reply-To: <013b01cc9413$7f134300$e001a8c0@cdd.loc> References: <20111026170726.01AD31541AF7@barracuda.crvinc.net> <013b01cc9413$7f134300$e001a8c0@cdd.loc> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32096B7E00@Mail2Node2.ad.uams.edu> We have two. One is about ten months old, the other a month and not a problem with either. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Wednesday, October 26, 2011 2:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histostar Embedding Station Hey Jessica, We have two Histostar's that have been here since March and have not had any issues with them. Can't say anything bad about them, yet. Thanks, Tim Subject: [Histonet] Histostar Embedding Station To: "Histonet@lists.utsouthwestern.edu" Message-ID: <1319563285.61798.YahooMailNeo@web121504.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Everyone, Just curious if anyone is using the Histostar Embedding Station? And I am curious to know how you like/dislike it? We have one and have been using it for about 4 weeks and are already needing parts. Thanks, Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From brett_connolly <@t> merck.com Thu Oct 27 09:31:54 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Oct 27 09:32:03 2011 Subject: [Histonet] Anti- guinea pig polymer secondary Message-ID: Hi all, Anyone aware of any anti-guinea pig secondary HRP polymer detection kits. I found one from Golden Bridge Int., Inc. and am asking if anyone has used it. Any other vendors making this?? Thanks, Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From pruegg <@t> ihctech.net Thu Oct 27 11:21:20 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Oct 27 11:21:27 2011 Subject: [Histonet] Anti- guinea pig polymer secondary In-Reply-To: References: Message-ID: <41497679F4734CEF83F07D633470291C@prueggihctechlt> Brett I just use a rabbit anti gp link and then follow with rab labeled polymer HRP, I get links from Southern Biotech. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Thursday, October 27, 2011 8:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Anti- guinea pig polymer secondary Hi all, Anyone aware of any anti-guinea pig secondary HRP polymer detection kits. I found one from Golden Bridge Int., Inc. and am asking if anyone has used it. Any other vendors making this?? Thanks, Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arlange <@t> medicine.nevada.edu Thu Oct 27 11:46:00 2011 From: arlange <@t> medicine.nevada.edu (Alicia R. Lange) Date: Thu Oct 27 11:46:13 2011 Subject: [Histonet] RE: Histonet Digest, Vol 95, Issue 31 In-Reply-To: <201110261702.p9QH1box021847@pps.splitter> References: <201110261702.p9QH1box021847@pps.splitter> Message-ID: <9809822F80B1414C90EB4438713C8968EC4DCF@MEDX.medicine.nevada.edu> Hi Kevin, I currently have the Leica IPS and IPC labeling system. There are some definitely pros/cons: Pros: Great for high volume Has convenient tray loading attachment (optional) Cons: Takes up a lot of space Very particular for type of slides used (may be a issue for cost saving purposes) High costs for service plans, would have to compare prices to other models (pm's, maintenance etc) Cassettes sometimes create static through the printer and prevent from ejecting out resulting in jamming I have used this system in a low volume and high volume lab. They haven't given me too much trouble. The problems I have stated I have encountered within the 3 years I have been working with them. Let me know if you need more information! Alicia Lange Laboratory Supervisor University of Nevada, Reno Pathology Department arlange@medicine.nevada.edu ------------------------------ Message: 2 Date: Tue, 25 Oct 2011 13:52:28 -0400 From: "Kevin Reif" Subject: [Histonet] Hi Group: To: Message-ID: <4EA6BF1D.2151.00B6.0@stegh.on.ca> Content-Type: text/plain; charset=US-ASCII Hi Group: Looking for opinions on cassette and slide labellers?? If you are using one please let me know the make, model and what you think of the machine, good or bad. Thanks in advance for all your input. Kevin Reif Senior Technologist - Pathology St. Thomas-Elgin General Hospital St. Thomas, On. Can. __________________________________________________________________________ St. Thomas Elgin General Hospital 189 Elm St. St. Thomas Ontario N5R 5C4 (519) 631-2020 http://www.stegh.on.ca Proud recipients of 2010 OHA Quality Healthcare Workplace Award - Platinum __________________________________________________________________________ Confidentiality/Privacy Notice: This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. For more information on our Privacy Policy, please see our website at http://www.stegh.on.ca ------------------------------ Message: 3 Date: Tue, 25 Oct 2011 10:58:04 -0700 (PDT) From: Steve Wong Subject: [Histonet] Please unsubscribe To: Histonet@lists.utsouthwestern.edu Message-ID: <1319565484.85941.YahooMailClassic@web65902.mail.ac4.yahoo.com> Content-Type: text/plain; charset=us-ascii Hello, Please unsubscribe me from this list. swongman@yahoo.com Thanks! ------------------------------ Message: 4 Date: Tue, 25 Oct 2011 12:59:41 -0500 From: "Harrison, Sandra C." Subject: [Histonet] saving slides between levels for IHC? To: "Webb, Dorothy L" , "Horge, Pamela J" , Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ------------------------------ Message: 5 Date: Tue, 25 Oct 2011 21:02:10 +0300 From: Badz Yahoo Subject: [Histonet] EMS 9000 To: "Histonet@lists.utsouthwestern.edu" Message-ID: <80FD49AB-B170-4680-B36E-EEECD98F2800@yahoo.com> Content-Type: text/plain; charset=us-ascii Hi histonetts..Anyone using the microwave machine EMS 9000?I appreciate if someone can give me some feedback..tanx ------------------------------ Message: 6 Date: Tue, 25 Oct 2011 11:05:10 -0700 From: "Morken, Timothy" Subject: [Histonet] RE: saving slides between levels for IHC? To: "'Harrison, Sandra C.'" , "Webb, Dorothy L" , "Horge, Pamela J" , "rollmann.denis@mayo.edu" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <8D7C2D242DBD45498006B21122072BF89448A30D@MCINFRWEM003.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Sandy, we have various protocols, some that have unstained between those to be stained. However, we have moved away from having "extras" cut "just in case" because we had thousands of unstained slides stored that were never used. After a few months they may be unreliable for staining. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Tuesday, October 25, 2011 11:00 AM To: Webb, Dorothy L; Horge, Pamela J; rollmann.denis@mayo.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] saving slides between levels for IHC? Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 25 Oct 2011 15:15:23 -0400 From: "Angela Bitting" Subject: Re: [Histonet] saving slides between levels for IHC? To: "Pamela J Horge" , "Dorothy L Webb" , , "Sandra C. Harrison" Cc: histonet@lists.utsouthwestern.edu Message-ID: <4EA6D28B.2B7F.00C9.1@geisinger.edu> Content-Type: text/plain; charset=US-ASCII we do for certain part types. sentinel lymph nodes for melanoma, renal bxs, liver core bxs, prostate needle core bxs. >>> "Harrison, Sandra C." 10/25/2011 1:59 PM >>> Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------ Message: 8 Date: Tue, 25 Oct 2011 21:44:11 +0100 (BST) From: Rhonda Henshall-Powell Subject: [Histonet] Seeking Field Application Specialists and Education Coordinator for CA-based Cancer Diagnostics Company To: "Histonet@lists.utsouthwestern.edu" Message-ID: <1319575451.47919.YahooMailNeo@web29501.mail.ird.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello Histonetters! ? Here I am again -?An industry-leading IHC/ISH diagnostics company is seeking an in-house Education Coordinator and two Field Application Specialists (Midwest and Northwest) requiring significant travel: Field Applications Specialist Provide expert level technical support knowledge of Anatomic Pathology principles to troubleshoot and support instrumentation, software, and train lab personnel on use, optimisation and validation of IHC and ISH reagents. If interested or know of someone who may be interested, please reply directly to me (rlhenshall_powell@yahoo.co.uk) for more information. ? Thank you ? Best Regards, Rhonda ------------------------------ Message: 9 Date: Tue, 25 Oct 2011 15:09:32 -0700 From: Patrick Laurie Subject: Re: [Histonet] saving slides between levels for IHC? To: Angela Bitting Cc: Pamela J Horge , rollmann.denis@mayo.edu, Dorothy L Webb , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We tend to take extras between levels especially on scant or needle biopsy tissue. We do waste several thousand slides per year, but by doing this it allows us to give the pathologist several representative sections and also save tissue for future IHC and special stains. On Tue, Oct 25, 2011 at 12:15 PM, Angela Bitting wrote: > we do for certain part types. sentinel lymph nodes for melanoma, renal bxs, > liver core bxs, prostate needle core bxs. > > >>> "Harrison, Sandra C." 10/25/2011 1:59 PM >>> > Does anyone know if it is standard to save sections from between the > levels for IP? Is it standard at your institution? > > > > > > Sandy C. Harrison, HTL (ASCP) > > Histology Supervisor > > Minneapolis VA > > 612-467-2449 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. It is > intended solely for the addressee. Access to this message by anyone else is > unauthorized. If you are not the intended recipient, any disclosure, > copying, distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you have received this > message in error, please delete all electronic copies of this message (and > the documents attached to it, if any), destroy any hard copies you may have > created and notify me immediately by replying to this email. Thank you. > > Geisinger Health System utilizes an encryption process to safeguard > Protected Health Information and other confidential data contained in > external e-mail messages. If email is encrypted, the recipient will receive > an e-mail instructing them to sign on to the Geisinger Health System Secure > E-mail Message Center to retrieve the encrypted e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com ------------------------------ Message: 10 Date: Wed, 26 Oct 2011 07:51:56 +0100 From: "Hobbs, Carl" Subject: [Histonet] Re: Seeking Beta-Amyloid not needing FA pretreatment To: "histonet@lists.utsouthwestern.edu" Message-ID: <11D9615B89C10747B1C985966A63D7CA3826477A48@KCL-MAIL04.kclad.ds.kcl.ac.uk> Content-Type: text/plain; charset="us-ascii" Be careful: HIER may expose not only extracellular amyloid plaques but also intracellular APP ( or...is it intracellular A beta ???? ;-)) Eg: with 6E10.... it is directed against an epitope found in APP and also A beta: if you do Formic acid, only extracellular plaques are positive...after HIER, there is also intracellular positivity. In my experience. Some examples of this can be found here, in the image gallery : http://www.immunoportal.com/ Whichever way you go....good luck. Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 ------------------------------ Message: 11 Date: Wed, 26 Oct 2011 07:28:54 -0400 From: "Sheila Fonner" Subject: RE: [Histonet] saving slides between levels for IHC? To: "'Harrison, Sandra C.'" , Message-ID: <000001cc93d2$71a7f670$54f7e350$@com> Content-Type: text/plain; charset="US-ASCII" Sandy, I don't think it is a "standard" procedure. However, our pathologist will frequently ask us to keep intervening levels for possible IHC on certain cases that are small or where the tumor is about to be exhausted. We are a dermatopathology lab. When it is requested, we cut the whole case on a clean waterbath, using distilled water and no additives, on plus slides. The routine H&E's are stained normally and the other slides are dried and held for possible immunos. Sheila KDL Pathology Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Tuesday, October 25, 2011 2:00 PM To: Webb, Dorothy L; Horge, Pamela J; rollmann.denis@mayo.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] saving slides between levels for IHC? Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 26 Oct 2011 09:42:18 -0400 From: "Connolly, Brett M" Subject: [Histonet] RE: Re: Seeking Beta-Amyloid not needing FA pretreatment To: "Hobbs, Carl" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Interesting....we see APP on frozen sections with 6E10, but not in paraffin sections after citrate HIER. We use the monoclonal from Covance, 1:12000 overnight at 4C. Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl Sent: Wednesday, October 26, 2011 2:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Seeking Beta-Amyloid not needing FA pretreatment Be careful: HIER may expose not only extracellular amyloid plaques but also intracellular APP ( or...is it intracellular A beta ???? ;-)) Eg: with 6E10.... it is directed against an epitope found in APP and also A beta: if you do Formic acid, only extracellular plaques are positive...after HIER, there is also intracellular positivity. In my experience. Some examples of this can be found here, in the image gallery : http://www.immunoportal.com/ Whichever way you go....good luck. Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 13 Date: Wed, 26 Oct 2011 08:49:26 -0500 From: Roger Brown Subject: [Histonet] Please remove my name To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello, Please remove my name from the Histonet mailing list. Thank you, Roger Brown (rbrown@mst.edu) ------------------------------ Message: 14 Date: Wed, 26 Oct 2011 09:56:15 -0400 From: "Vanessa J. Phelan" Subject: [Histonet] Please remove my name To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, please remove my name from the mailing list. Thank you ------------------------------ Message: 15 Date: Wed, 26 Oct 2011 07:32:57 -0700 From: Bea DeBrosse-Serra Subject: [Histonet] RE: Please remove my name To: "'Roger Brown'" , "Histonet@lists.utsouthwestern.edu" Message-ID: <493CAA64F203E14E8823737B9EE0E25F091E91273E@EXCHMB01.isis.local> Content-Type: text/plain; charset="us-ascii" If you want to be removed from the list, you have to do it yourself by going to the website below. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Brown Sent: Wednesday, October 26, 2011 6:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Please remove my name Hello, Please remove my name from the Histonet mailing list. Thank you, Roger Brown (rbrown@mst.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 26 Oct 2011 08:10:43 -0700 (PDT) From: Kim Donadio Subject: Re: [Histonet] saving slides between levels for IHC? To: Sheila Fonner , "'Harrison, Sandra C.'" , "histonet@lists.utsouthwestern.edu" Message-ID: <1319641843.94105.YahooMailNeo@web112302.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I think it depends on what the specimen is. All of the places Ive worked have always kept extras(levels) on sentinal nodes for keratins, also for prostate needle biopsies for p63/504s or other IHC's. ? Really doesnt it come down to what the Pathologist needs to make a diagnosis for the patient? ? I know it's a pain to cut so many levels and save them. Its also costly. But trust me, none of that is as bad as the sinking feeling you get when a biopsy has been exhausted and they need more stains :( ? Hope this helps. Just my 2 cents worth....... ? Kim Donadio ________________________________ From: Sheila Fonner To: "'Harrison, Sandra C.'" ; histonet@lists.utsouthwestern.edu Sent: Wednesday, October 26, 2011 7:28 AM Subject: RE: [Histonet] saving slides between levels for IHC? Sandy, I don't think it is a "standard" procedure.? However, our pathologist will frequently ask us to keep intervening levels for possible IHC on certain cases that are small or where the tumor is about to be exhausted.? We are a dermatopathology lab.? When it is requested, we cut the whole case on a clean waterbath, using distilled water and no additives, on plus slides. The routine H&E's are stained normally and the other slides are dried and held for possible immunos. Sheila KDL Pathology Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Tuesday, October 25, 2011 2:00 PM To: Webb, Dorothy L; Horge, Pamela J; rollmann.denis@mayo.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] saving slides between levels for IHC? Does anyone know if it is standard? to save sections from between the levels for IP?? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 26 Oct 2011 12:00:59 -0400 From: "Sheila Fonner" Subject: [Histonet] EBER ISH To: , "'Paul Googe'" , "'Susan Foreman'" Message-ID: <001901cc93f8$74289190$5c79b4b0$@com> Content-Type: text/plain; charset="us-ascii" Histonetters, Is anyone out there using the EBER ISH probes from Ventana on the Ultra Platform? I have a few questions. Did you need to get new software installed? Was there any training involved? And do you have successful protocols that you are willing to share? I have asked the questions before, but at the time there was nobody actually using it. I know that there were inquiries about the protocols because of the ASR status and the fact that Ventana cannot give you any advice. Any and all information would be greatly appreciated since we are wanting to start it up at our facility. Thanks, Sheila KDL Pathology Knoxville, TN ------------------------------ Message: 18 Date: Wed, 26 Oct 2011 12:04:28 -0400 From: "Sheila Fonner" Subject: [Histonet] Antibody questions To: Message-ID: <001e01cc93f8$f085e120$d191a360$@com> Content-Type: text/plain; charset="us-ascii" Is there anyone who is doing myc, Bap1, Pten, or CD123 for dermpath specimens, and who is your vendor? Do you like the products, and what platform are you using? Thanks for any information you may have. These are new requests from our pathologist. Sheila ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 95, Issue 31 **************************************** From cebass <@t> buffalo.edu Thu Oct 27 12:08:19 2011 From: cebass <@t> buffalo.edu (Bass, Caroline) Date: Thu Oct 27 12:08:24 2011 Subject: [Histonet] Microm HM 560 vacutome for brain sections? Message-ID: <0B0D2FF1-B8D2-4CF5-93DF-B107D49C5330@buffalo.edu> Hello Everyone, I'm thinking about purchasing a cryostat. I will most likely section primarily rodent brains and I'm concerned about wrinkling. One rep suggested the vacutome system, which according to the literature "the patented vacutome stretches sections to eradicate artifacts". I was also considering the cryojane system, which I have seen in action and think it would work well. Though I may also obtain mRNA from these sections and I wonder how the UV curing part of the system could affect things. Does anyone have experience with the vacutome and can suggest whether this is a good way to go. Or is the Cryojane better? I'm also considering some used Leicas, from the 1800, 1850 and 1900. My needs are fairly minimal, it will get weekly and possibly daily use on occasion, but for the most part I don't need all of the bells and whistles, just something that can cut brain fairly well. Thanks, Caroline From cweston <@t> valasciences.com Thu Oct 27 12:30:40 2011 From: cweston <@t> valasciences.com (Claire Weston) Date: Thu Oct 27 12:35:50 2011 Subject: [Histonet] Stripping antibodies Message-ID: <002401cc94ce$2619ca60$724d5f20$@com> Hi! Does anyone have experience in IHC stripping antibodies from a tissue section and re-probing with different antibodies? What is the best way to do that? If you could share your stripping protocol or experience I would appreciate it - thanks! Claire Claire Weston, PhD Research Scientist Vala Sciences, Inc. San Diego, CA 92121 From daniel.sjolander <@t> gmail.com Thu Oct 27 12:43:15 2011 From: daniel.sjolander <@t> gmail.com (Daniel Sjolander) Date: Thu Oct 27 12:43:40 2011 Subject: [Histonet] Re: Seeking Beta-Amyloid not needing FA pretreatment In-Reply-To: <11D9615B89C10747B1C985966A63D7CA3826477A48@KCL-MAIL04.kclad.ds.kcl.ac.uk> References: <11D9615B89C10747B1C985966A63D7CA3826477A48@KCL-MAIL04.kclad.ds.kcl.ac.uk> Message-ID: Why would you like to avoid detection of intracellular Abeta inclusions? //Daniel On Wed, Oct 26, 2011 at 8:51 AM, Hobbs, Carl wrote: > > Be careful: HIER may expose not only extracellular amyloid plaques but also > intracellular APP ( or...is it intracellular A beta ???? ;-)) > Eg: with 6E10.... it is directed against an epitope found in APP and also > A beta: if you do Formic acid, only extracellular plaques are > positive...after HIER, there is also intracellular positivity. > In my experience. > Some examples of this can be found here, in the image gallery : > http://www.immunoportal.com/ > > > Whichever way you go....good luck. > > Carl > > > Carl Hobbs > Histology Manager > Wolfson CARD > School of Biomedical Sciences > Kings College London > Guys Campus > SE1 1UL > Tel: 020 78486813 > Fax: 020 78486816 > 020 78486813 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cebass <@t> buffalo.edu Thu Oct 27 12:57:44 2011 From: cebass <@t> buffalo.edu (Bass, Caroline) Date: Thu Oct 27 12:57:47 2011 Subject: [Histonet] used cryostat opinion Message-ID: Hi Guys, I'm looking to section rat brain. A cryostat is not a huge necessity for my lab, I can borrow access from another. But it would be a nice item to have and I'm considering a few refurbished models, a Leica CM1800, CM1850 and CM1900. The difference in price between the 1800 and 1900 is $6500. Any suggestions here? Are there any features of the 1850 or 1900 that would really justify the leap in cost, considering that I will not be a heavy user? Thanks, Caroline From dencrowl <@t> MIT.EDU Thu Oct 27 12:59:55 2011 From: dencrowl <@t> MIT.EDU (Denise G Crowley) Date: Thu Oct 27 13:00:00 2011 Subject: [Histonet] oops Message-ID: <79C7A71C-E3D2-4A99-8B70-5A8CF90D5B9B@mit.edu> Hi all, Bad day here. Left the IHC stainer to attend a meeting and the volume of the DAB was not sufficient to stain all slides. By the time I realized this, they had been counterstained. I immediately applied DAB to the affected slides but naturally the antigen is not abundant if present at all. Will this work or does the hematoxylin counterstain interfere with the peroxidase reaction? Denise Crowley Histology Facility Manager Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu From chapcl <@t> yahoo.com Thu Oct 27 13:03:00 2011 From: chapcl <@t> yahoo.com (William) Date: Thu Oct 27 13:03:06 2011 Subject: [Histonet] oops In-Reply-To: <79C7A71C-E3D2-4A99-8B70-5A8CF90D5B9B@mit.edu> References: <79C7A71C-E3D2-4A99-8B70-5A8CF90D5B9B@mit.edu> Message-ID: Oops. Yes hematoxylin staining interferes with DAB staining. Sorry! You can save the slides by re-retrieving and restaining. Will Sent from my iPhone On Oct 27, 2011, at 10:59 AM, Denise G Crowley wrote: > Hi all, > > Bad day here. Left the IHC stainer to attend a meeting and the volume of the DAB was not sufficient to stain all slides. By the time I realized this, they had been counterstained. I immediately applied DAB to the affected slides but naturally the antigen is not abundant if present at all. Will this work or does the hematoxylin counterstain interfere with the peroxidase reaction? > > Denise Crowley > Histology Facility Manager > Koch Center for Integrative Cancer Research > Massachusetts Institute of Technology > 500 Main St. 76-182 > Cambridge MA 02139 > 617-258-8183 > dencrowl@mit.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amario3 <@t> uic.edu Thu Oct 27 13:14:50 2011 From: amario3 <@t> uic.edu (Andrea Marion) Date: Thu Oct 27 13:14:56 2011 Subject: [Histonet] Stripping antibodies Message-ID: Hi Claire, I'm also interested in re-probing for immunostains. If you receive any advice off-list, I would be happy to hear about it. I have an experiment that I may need to try this for, so I have done some preliminary research on the topic, but have not tried it yet. In principle, it seems reasonable to do. The only concern would be breaking the antibody-antigen interaction, without using some harsh buffer/detergent that would harm your epitope of interest. I plan to use 5 10 min washes of PBST, 0.5% -1% Tween-20 at room temperature. My reasoning is that 0.1% Tween is used routinely for washes in between antibody incubations for both Western blots and immunostains. I've mistakenly used 0.5% Tween for Western blot washing before, and it stripped the primary antibody :) If you have a membrane bound antigen, I wouldn't use detergent stripping. Either way I would confirm that your protocol works for each antibody you want to try - after stripping and washing, re-incubate with secondary antibody and see if you have any signal. This is how it is routinely done for Western blots. All of this is also presuming you are working with fluorescence detection; if you are using IHC with DAB, you will not be able to get the chromogen off the tissue even if you do remove the antibody. Invitrogen has a collection of protocols here: http://molecularprobestechnologynetwork.community.invitrogen.com/docs/DOC-1018 Let me know of your results & good luck! -Andrea Andrea Marion Graduate Student University of Illinois at Chicago [Histonet] Stripping antibodies Claire Weston cweston <@t> valasciences.com Thu Oct 27 12:30:40 CDT 2011 Previous message: [Histonet] Microm HM 560 vacutome for brain sections? Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] Hi! Does anyone have experience in IHC stripping antibodies from a tissue section and re-probing with different antibodies? What is the best way to do that? If you could share your stripping protocol or experience I would appreciate it - thanks! Claire Claire Weston, PhD Research Scientist Vala Sciences, Inc. San Diego, CA 92121 From nelsonrnch <@t> verizon.net Thu Oct 27 13:29:25 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Thu Oct 27 13:29:28 2011 Subject: [Histonet] CPT CODE Message-ID: <1319740165.76500.YahooMailNeo@web84517.mail.ne1.yahoo.com> Does anyone know the CPT code for Anal pap smear THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) nelsonrnch@verizon.net ? ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. From Lynne.Bell <@t> cvmc.org Thu Oct 27 13:59:30 2011 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Thu Oct 27 13:59:39 2011 Subject: [Histonet] CPT CODE In-Reply-To: <1319740165.76500.YahooMailNeo@web84517.mail.ne1.yahoo.com> References: <1319740165.76500.YahooMailNeo@web84517.mail.ne1.yahoo.com> Message-ID: Patti, We use CPT code 88112 - Cytopathology, selective cellular enhancement technique with interpretation (e.g. liquid based slide preparation method) except cervical or vaginal. (CPT 88112) Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SHANE NELSON Sent: Thursday, October 27, 2011 2:29 PM To: Histonet Subject: [Histonet] CPT CODE Does anyone know the CPT code for Anal pap smear THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) nelsonrnch@verizon.net ? ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Oct 27 14:04:35 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Oct 27 14:04:41 2011 Subject: [Histonet] oops In-Reply-To: References: <79C7A71C-E3D2-4A99-8B70-5A8CF90D5B9B@mit.edu> Message-ID: <2DCA337199D443C49FB61B07520DE801@prueggihctechlt> I have done cs before DAB lots of times with no problems, the only time I would expect a problem would be if your antigen is expressed in the nuclei and the cs is too dark. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William Sent: Thursday, October 27, 2011 12:03 PM To: Denise G Crowley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] oops Oops. Yes hematoxylin staining interferes with DAB staining. Sorry! You can save the slides by re-retrieving and restaining. Will Sent from my iPhone On Oct 27, 2011, at 10:59 AM, Denise G Crowley wrote: > Hi all, > > Bad day here. Left the IHC stainer to attend a meeting and the volume of the DAB was not sufficient to stain all slides. By the time I realized this, they had been counterstained. I immediately applied DAB to the affected slides but naturally the antigen is not abundant if present at all. Will this work or does the hematoxylin counterstain interfere with the peroxidase reaction? > > Denise Crowley > Histology Facility Manager > Koch Center for Integrative Cancer Research > Massachusetts Institute of Technology > 500 Main St. 76-182 > Cambridge MA 02139 > 617-258-8183 > dencrowl@mit.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From monettes <@t> mskcc.org Thu Oct 27 14:52:03 2011 From: monettes <@t> mskcc.org (monettes@mskcc.org) Date: Thu Oct 27 14:52:23 2011 Subject: [Histonet] Abs for detecting immune cells in mouse FFPE sections In-Reply-To: References: <1319656942.77550.YahooMailNeo@web125302.mail.ne1.yahoo.com> Message-ID: These are two good databases for antibodies and protocols for murine IHC: From the pathology lab at The Jackson Lab: http://tumor.informatics.jax.org/html/antibodies.html From the animal pathology lab at NCI: http://web.ncifcrf.gov/rtp/lasp/phl/immuno/ Best, Sebastien S?bastien Monette, DMV, MVSc, DACVP Comparative Pathologist Tri-Institutional Laboratory of Comparative Pathology Center of Comparative Medicine and Pathology Memorial Sloan-Kettering Cancer Center Weill Cornell Medical College The Rockefeller University 1275 York Avenue, Box 270, New York NY 10065 Phone: 646-888-2420 Fax: 646-422-0139 Email MSKCC: monettes@mskcc.org Email WCMC: sem2010@med.cornell.edu Web site: http://www.mskcc.org/mskcc/html/92361.cfm -----Original Message----- From: Yu, Jian [mailto:YuJ2@upmc.edu] Sent: Wednesday, October 26, 2011 3:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Abs for detecting immune cells in mouse FFPE sections Dear all, It will be greatly appreciated if you can share experience/information on the Abs against immune cells (vendor and cat#) that work for IHC or IF using mouse paraffin sections. Info on alternative Abs for these populations can be helpful too. I have a small research lab with limited budget for buying new Abs. Gr-1 (granulocytes) B220 (B cells) CD3 (T cells) Thank you! Jian Yu, Ph.D. University of Pittsburgh ===================================================================== Please note that this e-mail and any files transmitted with it may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer. From PAMarcum <@t> uams.edu Thu Oct 27 15:13:50 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Oct 27 15:14:27 2011 Subject: [Histonet] UAMS Little Rock AR Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32096B8073@Mail2Node2.ad.uams.edu> Good Morning, We are looking for an ASCP registered Histologist for a fulltime position at the University of Arkansas for Medical Sciences in Little Rock. We have an opening for an early morning shift person who is HT registered with clinical experience. We have great equipment with more changes coming. This is a chance to learn and grow with the Histology Department at UAMS. There will be some weekend rotations on Saturday only approximately every fourth week. The benefits are great for health, dental and retirement is matching up to 10% for your contributions by UAMS. We have 11 paid holidays each year and two weeks of vacation with sick time allotted per month. We cannot pay relocation or bonuses as this is a state run University site and we cannot use a recruiter, so please do not ask. I have tried and it is no recruiters. Please see the UAMS website for Jobs under technical and submit a resume. You may also call me for details or other information however: I cannot officially interview anyone without a submission through HR. See UAMS.edu Best Regards, Pamela A Marcum AP Supervisor Histology Slot 502 4301 W Markham Street Little Rock AR 72205 Office: 501-686-7554 Fax: 501-686-7151 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From mamawooo <@t> hotmail.com Thu Oct 27 16:29:34 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Thu Oct 27 16:29:39 2011 Subject: [Histonet] CPT CODE In-Reply-To: <1319740165.76500.YahooMailNeo@web84517.mail.ne1.yahoo.com> References: <1319740165.76500.YahooMailNeo@web84517.mail.ne1.yahoo.com> Message-ID: Shane, It depends on the prep you use. If it is a smear it should be 88104, if a thin prep 88312. Jan Omaha > Date: Thu, 27 Oct 2011 11:29:25 -0700 > From: nelsonrnch@verizon.net > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] CPT CODE > > Does anyone know the CPT code for Anal pap smear > > > THANK YOU, > > PATTI RUBEN-NELSON H.T.(ASCP) > nelsonrnch@verizon.net > > > CONFIDENTIALITY NOTICE: > This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants > and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable > law. Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful. If you are not > the addressee, please promptly delete this message and notify the sender of > the delivery error by e-mail or you may call 909-841-9761. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mamawooo <@t> hotmail.com Thu Oct 27 16:30:34 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Thu Oct 27 16:30:38 2011 Subject: [Histonet] CPT CODE In-Reply-To: <1319740165.76500.YahooMailNeo@web84517.mail.ne1.yahoo.com> References: <1319740165.76500.YahooMailNeo@web84517.mail.ne1.yahoo.com> Message-ID: Sorry, non-gyn thin prep, 88112. Jan > Date: Thu, 27 Oct 2011 11:29:25 -0700 > From: nelsonrnch@verizon.net > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] CPT CODE > > Does anyone know the CPT code for Anal pap smear > > > THANK YOU, > > PATTI RUBEN-NELSON H.T.(ASCP) > nelsonrnch@verizon.net > > > CONFIDENTIALITY NOTICE: > This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants > and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable > law. Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful. If you are not > the addressee, please promptly delete this message and notify the sender of > the delivery error by e-mail or you may call 909-841-9761. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c_m_hernandezjr <@t> yahoo.com Thu Oct 27 20:50:32 2011 From: c_m_hernandezjr <@t> yahoo.com (Carlos Hernandez) Date: Thu Oct 27 20:50:36 2011 Subject: [Histonet] Fellow Dermpath Lab Managers Message-ID: <1319766632.92785.YahooMailNeo@web31807.mail.mud.yahoo.com> Hello All! Wondering if anyone would be willing to email me privately to discuss salaries for Dermpath Lab Managers. ?My docs have been getting info from a lot of other docs out there as to what they pay their Lab Managers and I was hoping to do the same. If anyone has any other ideas on how to get some information regarding this I am all ears. Thanks in advance for all your help! Carlos From jkiernan <@t> uwo.ca Fri Oct 28 00:51:47 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Oct 28 00:51:56 2011 Subject: [Histonet] Carmine Message-ID: <7590a5f967624.4eaa0ab3@uwo.ca> Thanks, Peggy for drawing attention to the "Perfect Red" book. Must check it out! Carmine in histology wasn't always just mucicarmine. For many years carmine was the only stain. Corti used it to find the sensory organ for hearing in the cochlea (the dye was an aid to microdissection), and much of descriptive embryology comes from carmine-stained serial sections. The preparations keep for ever, with red nuclei and pink cytoplasm. There are many general-purpose carmine staining methods, and a few survive as red nuclear counterstains. John Kiernan Anatomy, UWO London, Canada = = = On 23/10/11, Lee & Peggy Wenk wrote: > > Just for fun: Check to see if your Sunday comic section carries Jump Start. Check out today?s 10/23/11 strip. > > Jump Start, a comic strip about a couple (policeman and nurse) and their kids, has the oldest girl wanting to be a cochineal insect for Halloween ? which is where histology gets carmine dye for the mucicarmine stain. > http://www.gocomics.com/jumpstart/2011/10/23 > > If you want to read a fascinating book about the the role of carmine in the exploration of the America?s, enslaving the people of Central and South America, pirates stealing ships loaded with the dye, spying, politics, government and religion, and the ?unions? of the dyeing industry back then ? find or buy a copy of ?A Perfect Red: Empire, Espionage, and the Quest for the Color of Desire? by Amy Butler Greenfield, 2006. > > If you want a more abbreviated version, Anatech?s newsletter ?The Innovator? had an article about carmine in their Winter 2007 issue ? all about the history, and about why the quality of Mucicarmine has gone downhill in the past few years. (And also towards the end of the newsletter - what Anatech has done to try to improve the quality of the carmine. The article includes promoting their version of mucicarmine, so just a head?s up ? this is their newsletter to promote their products. But they do a great job at educating in general, too. So I enjoy reading and learning from their newsletters. Great photos of what stains SHOULD look like.) > http://www.anatechltdusa.com/Innovators/Innovator12_06.pdf > > No ? I don?t get any money talking about the comic strip, the book or Anatech. I just think it?s neat to read about the history of dyes. And really great to to read about cochineal in a Sunday comic strip! > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Hospital > Royal Oak, MI 48073 > > (The opinions expressed are my own.) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From kcastillo <@t> frii.com Fri Oct 28 06:54:08 2011 From: kcastillo <@t> frii.com (kcastillo@frii.com) Date: Fri Oct 28 06:54:13 2011 Subject: [Histonet] SALARY Message-ID: HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY From Caroline.Pratt <@t> uphs.upenn.edu Fri Oct 28 08:21:01 2011 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Fri Oct 28 08:21:07 2011 Subject: [Histonet] SALARY In-Reply-To: References: Message-ID: <91FD001DCC6DC84EA6A4B12BB9313678016ED690@uphmasphi007.UPHS.PENNHEALTH.PRV> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From c_m_hernandezjr <@t> yahoo.com Fri Oct 28 09:07:29 2011 From: c_m_hernandezjr <@t> yahoo.com (Carlos Hernandez) Date: Fri Oct 28 09:07:36 2011 Subject: [Histonet] Histostar Embedding Station Message-ID: <1319810849.738.YahooMailNeo@web31807.mail.mud.yahoo.com> I've had mine for about a year and have had no problems either. Carlos From c_m_hernandezjr <@t> yahoo.com Fri Oct 28 09:43:59 2011 From: c_m_hernandezjr <@t> yahoo.com (Carlos Hernandez) Date: Fri Oct 28 09:44:04 2011 Subject: [Histonet] used cryostat opinion Message-ID: <1319813039.17904.YahooMailNeo@web31812.mail.mud.yahoo.com> I've had great success with the Microms and you can get some really good pricing on used units. ?That being said I've also used the Leica's and like the 1850. From STACEY.LANGENBERG <@t> UCDENVER.EDU Fri Oct 28 10:22:26 2011 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Fri Oct 28 10:22:33 2011 Subject: [Histonet] SALARY Message-ID: <5AF57FAF-B653-4833-9CBE-14EEACE931D0@ucdenver.edu> What about certification? Sent from myTouch 4G ----- Reply message ----- From: "Pratt, Caroline" To: "kcastillo@frii.com" , "Histonet@lists.utsouthwestern.edu" Subject: [Histonet] SALARY Date: Fri, Oct 28, 2011 7:24 am I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From sarah_taba <@t> yahoo.com Fri Oct 28 10:27:38 2011 From: sarah_taba <@t> yahoo.com (sarah Tabatabaei) Date: Fri Oct 28 10:28:42 2011 Subject: [Histonet] How to save dried antibody? In-Reply-To: <5AF57FAF-B653-4833-9CBE-14EEACE931D0@ucdenver.edu> References: <5AF57FAF-B653-4833-9CBE-14EEACE931D0@ucdenver.edu> Message-ID: <1319815658.47397.YahooMailNeo@web45713.mail.sp1.yahoo.com> Dear All, We have purchased a sheep anti-CGRP antibody for our fluorescent IHC study on human tissue and kept it in -20 for a while. It came in liquid but now it's all stiff and looks like it's being crystallized. I cannot pipet any of it. It has hardened and looks like a piece of glass at bottom of its container. I tried to re-suspend it with the same amount of glycerol, but the antibody doesn't seem to be mixing with it. Does anyone know how to save this antibody? How can I bring it back to its liquid state?? Thank you for your time ? Sarah Sadat, DDS MSc Student, Dental Sciences McGill University Montreal QC. From one_angel_secret <@t> yahoo.com Fri Oct 28 10:48:34 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Oct 28 10:48:39 2011 Subject: [Histonet] How to save dried antibody? In-Reply-To: <1319815658.47397.YahooMailNeo@web45713.mail.sp1.yahoo.com> References: <5AF57FAF-B653-4833-9CBE-14EEACE931D0@ucdenver.edu> <1319815658.47397.YahooMailNeo@web45713.mail.sp1.yahoo.com> Message-ID: <1319816914.3678.YahooMailNeo@web112316.mail.gq1.yahoo.com> Not sure if others would call this a "lyophilized" situation. Dont know the specifics of what you initially recieved. Here's my best shot as I recall from using lyophilized antibodies doing IF for renal bx's a while back. ? Back then I used a PBS based diluent. A very low reconstitution amount, 50-100um. I typically used the diluent in the pipett tip to swirl the freeze dried/lyophilized antibody back together while warming the tube with my thumb as I held it. It worked well in that situation for me. ? Hope this helps. I'm sure there are others out here who do this every day and can offer more specifics? ? Best of luck. ? Kim Donadio ________________________________ From: sarah Tabatabaei To: "histonet@lists.utsouthwestern.edu" Sent: Friday, October 28, 2011 11:27 AM Subject: [Histonet] How to save dried antibody? Dear All, We have purchased a sheep anti-CGRP antibody for our fluorescent IHC study on human tissue and kept it in -20 for a while. It came in liquid but now it's all stiff and looks like it's being crystallized. I cannot pipet any of it. It has hardened and looks like a piece of glass at bottom of its container. I tried to re-suspend it with the same amount of glycerol, but the antibody doesn't seem to be mixing with it. Does anyone know how to save this antibody? How can I bring it back to its liquid state?? Thank you for your time ? Sarah Sadat, DDS MSc Student, Dental Sciences McGill University Montreal QC. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Fri Oct 28 10:56:45 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Oct 28 10:56:59 2011 Subject: [Histonet] How to save dried antibody? In-Reply-To: <1319815658.47397.YahooMailNeo@web45713.mail.sp1.yahoo.com> References: <5AF57FAF-B653-4833-9CBE-14EEACE931D0@ucdenver.edu> <1319815658.47397.YahooMailNeo@web45713.mail.sp1.yahoo.com> Message-ID: If the antibody has precipitated, it may be impossible to restore activity. The glycerol is present to keep the suspension in liquid form - and it sounds like you no longer have a liquid. Try adding an appropriate suspension buffer and keep your fingers crossed. If you do not have previous experience with this antibody and know what to expect regarding performance, I would discard it and re-order. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sarah Tabatabaei Sent: Friday, October 28, 2011 9:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How to save dried antibody? Dear All, We have purchased a sheep anti-CGRP antibody for our fluorescent IHC study on human tissue and kept it in -20 for a while. It came in liquid but now it's all stiff and looks like it's being crystallized. I cannot pipet any of it. It has hardened and looks like a piece of glass at bottom of its container. I tried to re-suspend it with the same amount of glycerol, but the antibody doesn't seem to be mixing with it. Does anyone know how to save this antibody? How can I bring it back to its liquid state?? Thank you for your time ? Sarah Sadat, DDS MSc Student, Dental Sciences McGill University Montreal QC. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dliaros <@t> BioReference.com Fri Oct 28 11:11:46 2011 From: dliaros <@t> BioReference.com (Donna Liaros) Date: Fri Oct 28 11:11:14 2011 Subject: [Histonet] Varicella Zoster Message-ID: <6CB019913E5F6940AD6E4871841EC5364C3901@mbx-3.biosvr1.bioreference.com> Anyone have any recommendations for a vendor that supplies a concentrated antibody for Varicella Zoster Virus (herpes zoster)? I would be optimizing it on the Ventana Ultra. The information transmitted in this email and any of its attachments is intended only for the person or entity to which it is addressed and may contain BioReference Laboratories proprietary information, which is privileged, confidential, or subject to copyright belonging to BioReference Laboratories. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited and may be unlawful. If you received this in error, please contact the sender immediately and delete and destroy the communication and all of the attachments you have received and all copies thereof. From 41dmb41 <@t> gmail.com Fri Oct 28 11:30:43 2011 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Fri Oct 28 11:30:50 2011 Subject: [Histonet] Varicella Zoster In-Reply-To: <6CB019913E5F6940AD6E4871841EC5364C3901@mbx-3.biosvr1.bioreference.com> References: <6CB019913E5F6940AD6E4871841EC5364C3901@mbx-3.biosvr1.bioreference.com> Message-ID: abcam has a few Varicella Zoster strains to choose from... I haven't used them before, so I can't speak of how well they work or provide much insight to dilutions and protocols for the Ventana, but the other antibodies I've used from them work well. Drew Sent from my iPhone On Oct 28, 2011, at 12:11 PM, Donna Liaros wrote: > Anyone have any recommendations for a vendor that supplies a concentrated antibody for Varicella Zoster Virus (herpes zoster)? I would be optimizing it on the Ventana Ultra. > The information transmitted in this email and any of its attachments is intended only for the person or entity to which it is addressed and may contain BioReference Laboratories proprietary information, which is privileged, confidential, or subject to copyright belonging to BioReference Laboratories. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited and may be unlawful. If you received this in error, please contact the sender immediately and delete and destroy the communication and all of the attachments you have received and all copies thereof. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Fri Oct 28 11:34:31 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Oct 28 11:34:37 2011 Subject: [Histonet] How to save dried antibody? References: <5AF57FAF-B653-4833-9CBE-14EEACE931D0@ucdenver.edu> <1319815658.47397.YahooMailNeo@web45713.mail.sp1.yahoo.com> Message-ID: <92B7E116DC514AE19E7BBADC23503947@auxs.umn.edu> I personally would not use it; you don't know how much loss of immunologic reactivity has occurred, and that might affect your project data. Buy a fresh supply, if possible. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "sarah Tabatabaei" To: Sent: Friday, October 28, 2011 10:27 AM Subject: [Histonet] How to save dried antibody? Dear All, We have purchased a sheep anti-CGRP antibody for our fluorescent IHC study on human tissue and kept it in -20 for a while. It came in liquid but now it's all stiff and looks like it's being crystallized. I cannot pipet any of it. It has hardened and looks like a piece of glass at bottom of its container. I tried to re-suspend it with the same amount of glycerol, but the antibody doesn't seem to be mixing with it. Does anyone know how to save this antibody? How can I bring it back to its liquid state? Thank you for your time Sarah Sadat, DDS MSc Student, Dental Sciences McGill University Montreal QC. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lhopson <@t> cellmarque.com Fri Oct 28 11:37:07 2011 From: lhopson <@t> cellmarque.com (Lauren Hopson) Date: Fri Oct 28 11:37:13 2011 Subject: [Histonet] Varicella Zoster In-Reply-To: References: <6CB019913E5F6940AD6E4871841EC5364C3901@mbx-3.biosvr1.bioreference.com> Message-ID: <44DA50B7F44F1A47B4F06C6025102E3401A2C8@cmrocmb2.cellmarque.local> Hi Histonet! Cell Marque offers a Varicella Zoster antibody in both concentrate and pre-dilute. Please contact your rep or customer service if you have any questions! Thank you, Lauren Hopson Technical Consultant 6600 Sierra College Blvd. Rocklin, CA 95677 (916) 746-8900 x8929 (916) 996-2353 (mobile) (916) 746-8989 (fax) www.cellmarque.com From Passion to Product to Patient.(r) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Friday, October 28, 2011 9:31 AM To: Donna Liaros Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Varicella Zoster abcam has a few Varicella Zoster strains to choose from... I haven't used them before, so I can't speak of how well they work or provide much insight to dilutions and protocols for the Ventana, but the other antibodies I've used from them work well. Drew Sent from my iPhone On Oct 28, 2011, at 12:11 PM, Donna Liaros wrote: > Anyone have any recommendations for a vendor that supplies a concentrated antibody for Varicella Zoster Virus (herpes zoster)? I would be optimizing it on the Ventana Ultra. > The information transmitted in this email and any of its attachments is intended only for the person or entity to which it is addressed and may contain BioReference Laboratories proprietary information, which is privileged, confidential, or subject to copyright belonging to BioReference Laboratories. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited and may be unlawful. If you received this in error, please contact the sender immediately and delete and destroy the communication and all of the attachments you have received and all copies thereof. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Fri Oct 28 11:52:23 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Oct 28 11:52:35 2011 Subject: [Histonet] SALARY In-Reply-To: <91FD001DCC6DC84EA6A4B12BB9313678016ED690@uphmasphi007.UPHS.PENNHEALTH.PRV> References: <91FD001DCC6DC84EA6A4B12BB9313678016ED690@uphmasphi007.UPHS.PENNHEALTH.PRV> Message-ID: <4EAAA586.7400.0077.1@harthosp.org> I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From plott <@t> uab.edu Fri Oct 28 12:12:51 2011 From: plott <@t> uab.edu (Patricia F Lott) Date: Fri Oct 28 12:12:57 2011 Subject: [Histonet] need help with TMA Message-ID: We have recently started doing tissue microarrays. The problem we have is that some of the small cores drop out after the first few sections. I think my problem is in the step where we are supposed to melt the donor and recipient blocks together. We tried 5 minutes at 37 degrees, and then minutes at 60 degrees x3, with cooling to room temp in between. Any suggestions? Thanks, Patty Lott, UAB From Wanda.Smith <@t> HCAhealthcare.com Fri Oct 28 12:15:15 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Oct 28 12:15:21 2011 Subject: [Histonet] OK We're Counting Down for SCSHT Meeting Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA2731F0A23F@NADCWPMSGCMS03.hca.corpad.net> OK All you SC and close to SC Histotechs!!!!! The fall meeting of the SCSHT is next weekend at Marina Inn at Grande Dunes in Myrtle Beach, SC. We only have 10 registrants right now, so I need to know who's coming but you just haven't sent in your registration!!!! It's fine, we want you to attend, we are trying to get a head count for the food, etc!!!! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From SJMccabe <@t> drmc.org Fri Oct 28 12:35:14 2011 From: SJMccabe <@t> drmc.org (McCabe, Sara J.) Date: Fri Oct 28 12:35:21 2011 Subject: [Histonet] PMS2 Mismatch Repair Message-ID: <02AE2390303AAB43A823930EAD6B63AE1C46B9E5@EX07.drmc.org> Hello Histonetters, Wondering if anyone is using PMS2 Mismatch Repair Enzyme from Novocastra/Leica. Have tried varied dilutions and retrievals without any luck. Any suggestions would be great! Thanks! Sara McCabe, BS, HT (ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. From tpodawiltz <@t> lrgh.org Fri Oct 28 12:44:52 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Oct 28 12:45:03 2011 Subject: [Histonet] SALARY In-Reply-To: <4EAAA586.7400.0077.1@harthosp.org> References: <91FD001DCC6DC84EA6A4B12BB9313678016ED690@uphmasphi007.UPHS.PENNHEALTH.PRV> <4EAAA586.7400.0077.1@harthosp.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323E5E7D251@LRGHEXVS1.practice.lrgh.org> I like your world. I have seen more places that try to price Histology in the same range as CLA's not even close to MLT's. Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 28, 2011 12:52 PM To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Caroline Pratt Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From cmhernandez78 <@t> me.com Fri Oct 28 12:45:18 2011 From: cmhernandez78 <@t> me.com (Carlos Hernandez) Date: Fri Oct 28 12:45:25 2011 Subject: [Histonet] SALARY Message-ID: <78A8BCC2-E2F2-4283-8DD5-ECA02C033A4E@me.com> Really good analogy Richard! From jwray78 <@t> gmail.com Fri Oct 28 12:57:22 2011 From: jwray78 <@t> gmail.com (Josh Wray) Date: Fri Oct 28 12:57:26 2011 Subject: [Histonet] Re: Histonet Digest, Vol 95, Issue 34 In-Reply-To: <4eaadfa9.0ff4960a.1cc8.409aSMTPIN_ADDED@mx.google.com> References: <4eaadfa9.0ff4960a.1cc8.409aSMTPIN_ADDED@mx.google.com> Message-ID: Richard, You looking to hire a free agent...oh I mean a lead or supervisor:) Joshua Wray HT(ASCP), QIHC Lead Technician, IHC Ameripath-Indianapolis > Message: 6 > Date: Fri, 28 Oct 2011 12:52:23 -0400 > From: "Richard Cartun" > Subject: RE: [Histonet] SALARY > To: ,, > ? ? ? ?"Caroline Pratt" > Message-ID: <4EAAA586.7400.0077.1@harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > I think that's low. ?If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. ?It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". ?Histotechnologists are the most valuable employees in the laboratory today! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT ?06102 > (860) 545-1596?Office > (860) 545-2204?Fax > > >>>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> > I would say $28 to $32 dollars an hour depending on experience and education. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com > Sent: Friday, October 28, 2011 7:54 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] SALARY > > HI EVERYONE, > > WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID > THESE DAYS. ?HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM > LAB AND DOING MOHS ALSO. ?THANKS FOR YOUR HELP. ?KRISTY From DKBoyd <@t> chs.net Fri Oct 28 13:04:14 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Oct 28 13:04:23 2011 Subject: [Histonet] Cytology Immediate Evals Message-ID: If you have 4 immediate evaluations during a FNA are you all charging 4 immed. eval. charges 88172 and an 88108 for each pass (cytospins)? Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Caroline.Pratt <@t> uphs.upenn.edu Fri Oct 28 13:18:59 2011 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Fri Oct 28 13:19:04 2011 Subject: [Histonet] SALARY In-Reply-To: <4EAAA586.7400.0077.1@harthosp.org> References: <91FD001DCC6DC84EA6A4B12BB9313678016ED690@uphmasphi007.UPHS.PENNHEALTH.PRV> <4EAAA586.7400.0077.1@harthosp.org> Message-ID: <91FD001DCC6DC84EA6A4B12BB9313678016ED6AB@uphmasphi007.UPHS.PENNHEALTH.PRV> If that is an option, I would agree, but non-profit teaching hospitals have compensation ranges and grids according to education and experience. We keep every employee with the same education and skill set at the same compensation for an equitable pay system. If we feel our employees are being under paid we will conduct a market analysis and the rates will be increased for all applicable employees if the market analysis justifies it. Car :) -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Friday, October 28, 2011 12:52 PM To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, Caroline Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------------------------------------------------ - This message was secured by ZixCorp(R). The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From jshelley <@t> sanfordburnham.org Fri Oct 28 13:25:04 2011 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Fri Oct 28 13:25:10 2011 Subject: [Histonet] SALARY In-Reply-To: <91FD001DCC6DC84EA6A4B12BB9313678016ED6AB@uphmasphi007.UPHS.PENNHEALTH.PRV> References: <91FD001DCC6DC84EA6A4B12BB9313678016ED690@uphmasphi007.UPHS.PENNHEALTH.PRV> <4EAAA586.7400.0077.1@harthosp.org> <91FD001DCC6DC84EA6A4B12BB9313678016ED6AB@uphmasphi007.UPHS.PENNHEALTH.PRV> Message-ID: <5A605CE38EECB64B94485C02125A0C4405C741AF53@LN-MAIL07.ln.burnham.org> With all that said what are your going rates in this area that you are speaking. Kind Regards! ? John J Shelley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Friday, October 28, 2011 2:19 PM To: Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY If that is an option, I would agree, but non-profit teaching hospitals have compensation ranges and grids according to education and experience. We keep every employee with the same education and skill set at the same compensation for an equitable pay system. If we feel our employees are being under paid we will conduct a market analysis and the rates will be increased for all applicable employees if the market analysis justifies it. Car :) -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Friday, October 28, 2011 12:52 PM To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, Caroline Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------------------------------------------------ - This message was secured by ZixCorp(R). The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Oct 28 13:48:50 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Oct 28 13:48:56 2011 Subject: [Histonet] SALARY In-Reply-To: <4EAAA586.7400.0077.1@harthosp.org> References: <91FD001DCC6DC84EA6A4B12BB9313678016ED690@uphmasphi007.UPHS.PENNHEALTH.PRV> <4EAAA586.7400.0077.1@harthosp.org> Message-ID: <8D7C2D242DBD45498006B21122072BF89448A345@MCINFRWEM003.ucsfmedicalcenter.org> It's all relative to your area and absolute dollars aren't applicable outside a given region. For instance, here in the San Francisco Bay Area we have so many biotech companies and large medical centers sopping up histotechs that the going rate for even minimal experience is about $35/hr. Long term employees and supervisors can make over $50/hr. However, the cost of living is sky high, and just about everyone commutes quite a ways so recruiting from outside the area is extremely difficult - once people see how expensive it is and how far out they may have to live to afford housing we end up with very few applicants. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 28, 2011 9:52 AM To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Caroline Pratt Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri Oct 28 14:22:15 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Oct 28 14:22:21 2011 Subject: [Histonet] SALARY In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323E5E7D251@LRGHEXVS1.practice.lrgh.org> References: <91FD001DCC6DC84EA6A4B12BB9313678016ED690@uphmasphi007.UPHS.PENNHEALTH.PRV><4EAAA586.7400.0077.1@harthosp.org> <38667E7FB77ECD4E91BFAEB8D986386323E5E7D251@LRGHEXVS1.practice.lrgh.org> Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFB7C@nmdamailsvr.nmda.ad.nmsu.edu> I nominate Dr. Cartun as the Patron Saint for Histopathology. We should work on a statue and a Mission Statement for him... From jason.madore <@t> gmail.com Fri Oct 28 14:31:48 2011 From: jason.madore <@t> gmail.com (Jason Madore) Date: Fri Oct 28 14:32:22 2011 Subject: [Histonet] antibody who's who? Message-ID: Hello, We are in the process of testing multiple antibodies against the same target(s). I can't figure out though if two companies are selling the same antibody, or rather, as I know they are repackaging and selling the same antibodies, I can't tell who selling who's antibody. If anyone has any suggestions they would be greatly appreciated. Jason From liz <@t> premierlab.com Fri Oct 28 14:39:19 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Oct 28 14:39:22 2011 Subject: [Histonet] antibody who's who? In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE011380AC6F66@SBS2K8.premierlab.local> Jason Look at the images, clone, working conditions, sample concentration and size of the antibodies if all of these conditions are the same, especially if they are showing the same image, then its likely that the antibodies are the same. If any of those conditions are different then they could be different antibodies. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason Madore Sent: Friday, October 28, 2011 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antibody who's who? Hello, We are in the process of testing multiple antibodies against the same target(s). I can't figure out though if two companies are selling the same antibody, or rather, as I know they are repackaging and selling the same antibodies, I can't tell who selling who's antibody. If anyone has any suggestions they would be greatly appreciated. Jason _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Oct 28 14:53:07 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Fri Oct 28 14:53:12 2011 Subject: [Histonet] SALARY Message-ID: That sounds good to me! I have never been fortunate enough to have education weighted. Of course I know it depends on market, location, and cost of living. Makes me want to research and pack up! Joelle Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Langenberg Stacey Date: Fri, 28 Oct 2011 15:22:26 To: ; ; Subject: Re: [Histonet] SALARY What about certification? Sent from myTouch 4G ----- Reply message ----- From: "Pratt, Caroline" To: "kcastillo@frii.com" , "Histonet@lists.utsouthwestern.edu" Subject: [Histonet] SALARY Date: Fri, Oct 28, 2011 7:24 am I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS.? HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO.? THANKS FOR YOUR HELP.? KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Wanda.Smith <@t> HCAhealthcare.com Fri Oct 28 15:11:37 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Oct 28 15:11:41 2011 Subject: [Histonet] SCSHT FALL MEETING NOV 4-5, 2011 Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA2731F0A4D4@NADCWPMSGCMS03.hca.corpad.net> Good afternoon, My last post had too many words in the title so you could not see it was about our fall meeting!!! If anyone is planning to come and has not registered, please email me and plan to come on!!!!! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From pruegg <@t> ihctech.net Fri Oct 28 17:15:14 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Oct 28 17:15:22 2011 Subject: [Histonet] SALARY In-Reply-To: References: Message-ID: <098426FCB4DA4862866421D26F8A6E3E@prueggihctechlt> I have been wondering what a new certified HTL in the Denver area might expect to be paid, this person has a couple of years of exclusive histology experience heavy on IHC, bright field and IF, and frozen sectioning expertise along with all other areas of histology. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Friday, October 28, 2011 1:53 PM To: Langenberg Stacey ; caroline.pratt@uphs.upenn.edu ; kcastillo@frii.com ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] SALARY That sounds good to me! I have never been fortunate enough to have education weighted. Of course I know it depends on market, location, and cost of living. Makes me want to research and pack up! Joelle Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Langenberg Stacey Date: Fri, 28 Oct 2011 15:22:26 To: ; ; Subject: Re: [Histonet] SALARY What about certification? Sent from myTouch 4G ----- Reply message ----- From: "Pratt, Caroline" To: "kcastillo@frii.com" , "Histonet@lists.utsouthwestern.edu" Subject: [Histonet] SALARY Date: Fri, Oct 28, 2011 7:24 am I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS.? HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO.? THANKS FOR YOUR HELP.? KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From turkekul <@t> gmail.com Fri Oct 28 18:58:42 2011 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Fri Oct 28 18:58:46 2011 Subject: [Histonet] fixation, tissue processing for paraffin embedding and sectiong of the mouse eye Message-ID: Anyone has a good protocol to fix, process, embed and section mouse eye? Prefered fixative is formalin. Any suggestions are welcome Thank you! Mesru Turkekul mskcc.org From Ramona_Nelson <@t> bd.com Fri Oct 28 20:08:28 2011 From: Ramona_Nelson <@t> bd.com (Ramona_Nelson@bd.com) Date: Fri Oct 28 20:08:37 2011 Subject: [Histonet] AUTO: Ramona Nelson is out of the office. (returning 11/01/2011) Message-ID: I am out of the office until 11/01/20 I Note: This is an automated response to your message &quo t;Histonet Digest, Vol 95, Issue 33" AM. This is the only notification you will receive wh person is away. _________________________________________________________________ ********************************************************** ********* IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This me group's products or services them. If this is such a message and you receiving future advertisements or solicitations f please forward this e-mail to optoutbygroup@bd.com. ******************************************************************* Th for the design proprietary informatio attorney-client privilege or other confidenti If you are not a designated recipient, you may not re or distribute this message. If you received this in error notify the sender by reply e-mail and delete this message. Thank you. ************************************************************* **** Dickinson and Comp U.S.A. ***************** ************************************************** From histonet.nospam <@t> vneubert.com Sat Oct 29 06:08:30 2011 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Sat Oct 29 06:08:42 2011 Subject: [Histonet] antibody who's who? In-Reply-To: References: Message-ID: <4EABDEAE.40704@vneubert.com> Hi! Why not just call the companies and ask them? On 28.10.2011 21:31, Jason Madore wrote: > Hello, > > We are in the process of testing multiple antibodies against the same > target(s). I can't figure out though if two companies are selling the > same antibody, or rather, as I know they are repackaging and selling > the same antibodies, I can't tell who selling who's antibody. If > anyone has any suggestions they would be greatly appreciated. > > Jason > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Sat Oct 29 11:20:50 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Sat Oct 29 11:20:40 2011 Subject: [Histonet] SALARY In-Reply-To: <91FD001DCC6DC84EA6A4B12BB9313678016ED6AB@uphmasphi007.UPHS.PENNHEALTH.PRV> References: <91FD001DCC6DC84EA6A4B12BB9313678016ED690@uphmasphi007.UPHS.PENNHEALTH.PRV> <4EAAA586.7400.0077.1@harthosp.org> <91FD001DCC6DC84EA6A4B12BB9313678016ED6AB@uphmasphi007.UPHS.PENNHEALTH.PRV> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F5F1E4@SMCMAIL01.somerset-healthcare.com> Our hospital works the same way. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Friday, October 28, 2011 2:19 PM To: Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY If that is an option, I would agree, but non-profit teaching hospitals have compensation ranges and grids according to education and experience. We keep every employee with the same education and skill set at the same compensation for an equitable pay system. If we feel our employees are being under paid we will conduct a market analysis and the rates will be increased for all applicable employees if the market analysis justifies it. Car :) -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Friday, October 28, 2011 12:52 PM To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, Caroline Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------------------------------------------------ - This message was secured by ZixCorp(R). The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From amosbrooks <@t> gmail.com Sat Oct 29 12:40:22 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Oct 29 12:40:41 2011 Subject: [Histonet] Stripping antibodies Message-ID: Hi, Stripping sites are usually found in the more seedy areas of large cities ... Oh wait you meant ... nevermind :-) If you developed the reaction with DAB, you may be in a difficult position. DAB is a really strong reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in 5% potassium premanganate for 5 min followed by 5% oxalic acid until it is clear (usually a couple of minutes). Unfortunately, I am not sure what effect this will have on the epitopes you are looking for, but this is usually what is used to clean precipitated DAB from instruments it should work on the tissue too. If you use another chromogen such as AEC it is much easier (to many people's dismay) to remove this end product. Using alkaline phosphatase will be easy to remove the chromogens as well. It would also be very easy to do this with fluorescent tags too. Just remove the coverslip and dip the slide in ethanol and it will remove the colored end product. Then just start over for the new antigen. Good luck, Amos On Fri, Oct 28, 2011 at 11:50 AM, wrote: > Message: 2 > Date: Thu, 27 Oct 2011 10:30:40 -0700 > From: "Claire Weston" > Subject: [Histonet] Stripping antibodies > To: > Message-ID: <002401cc94ce$2619ca60$724d5f20$@com> > Content-Type: text/plain; charset="us-ascii" > > Hi! > > > > Does anyone have experience in IHC stripping antibodies from a tissue > section and re-probing with different antibodies? What is the best way to > do that? If you could share your stripping protocol or experience I would > appreciate it - thanks! > > > > Claire > From sadey <@t> hotmail.ca Sat Oct 29 16:24:00 2011 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Sat Oct 29 16:24:04 2011 Subject: [Histonet] Pathos microwave processor Message-ID: Hi Everyone: I am looking for some opinions on the Pathos microwave processor. Thanks Sheila From cmhernandez78 <@t> me.com Sat Oct 29 16:33:01 2011 From: cmhernandez78 <@t> me.com (Carlos Hernandez) Date: Sat Oct 29 16:33:10 2011 Subject: [Histonet] Pathos microwave processor In-Reply-To: References: Message-ID: I have the Delta and I absolutely love it. I run all Derm, but my specimens come out great regardless whether it's a wide excision, cyst, or lipoma. I also love the fact that I don't have to use xylene or formalin. There is no clean cycle ad reagents last a lot longer especially the paraffin. I could go on and on! If you have any specific questions feel free to contact me. Carlos On Oct 29, 2011, at 3:24 PM, Sheila Adey wrote: > > Hi Everyone: > I am looking for some opinions on the Pathos microwave processor. > Thanks > Sheila _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sadey <@t> hotmail.ca Sat Oct 29 19:36:33 2011 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Sat Oct 29 19:36:37 2011 Subject: [Histonet] DISPOSAL OF SPECIMENS In-Reply-To: <002501cc9319$eddcd580$e001a8c0@cdd.loc> References: <20111024170731.DC5F2131284D@barracuda.crvinc.net>, <002501cc9319$eddcd580$e001a8c0@cdd.loc> Message-ID: As long as the specimen containers are being autoclaved or incinerated, there will be nothing readable left of the labels. > From: thiggins@cddmedical.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 25 Oct 2011 08:28:04 -0500 > Subject: [Histonet] DISPOSAL OF SPECIMENS > > I have been in the histology field for 20 years and never heard of having to > remove the labels prior to disposal through an approved vendor. I don't > believe that the labels from blood tubes are removed prior to disposal, this > would be the same type of scenario. > > Thanks, > > Tim > > > Message: 9 > Date: Mon, 24 Oct 2011 12:36 -0400 > From: "Sara Baldwin/mhhcc.org" > Subject: [Histonet] DISPOSAL OF SPECIMENS > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi histonetters > what does everyone currently do with the vials the Pathologists empty when > doing gross? Ours goes in biohazard bags, but someone mentioned to me they > the labels should be taken off and then the vials put in the biohazard bag. > If we did this we would just be taking off labels all day long. I have > worked at 2 hospitals in my 35 years of experience and we have never done > this. If we started this look at the clinical labs with blood specimens. > Isn't there some sort of confidential agreement between the hospitals and > hazard material companies? I would love to know your thoughts. > > > Thanks > Histology/Cytology Supervisor > S. Kathy Baldwin, SCT (ASCP) > Memorial Hospital and Health Care Center > sbaldwin@mhhcc.org > Ph 812-996-0210, 0216, Fax 812-996-0232, > Pager 812-481-0897, Cell 812-887-3357 > Confidential information, Authorized use only. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Sun Oct 30 11:09:58 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sun Oct 30 11:10:04 2011 Subject: [Histonet] Stripping antibodies In-Reply-To: References: Message-ID: <7640acec48487.4ead3e96@uwo.ca> Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow. If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents. Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want? This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody. Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars). The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios, 1999, is very good. John Kiernan Anatomy, UWO London, Canada = = = = On 29/10/11, Amos Brooks wrote: > > Hi, > Stripping sites are usually found in the more seedy areas of large > cities ... Oh wait you meant ... nevermind :-) If you developed the reaction > with DAB, you may be in a difficult position. DAB is a really strong > reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in > 5% potassium premanganate for 5 min followed by 5% oxalic acid until it is > clear (usually a couple of minutes). Unfortunately, I am not sure what > effect this will have on the epitopes you are looking for, but this is > usually what is used to clean precipitated DAB from instruments it should > work on the tissue too. > If you use another chromogen such as AEC it is much easier (to many > people's dismay) to remove this end product. Using alkaline phosphatase will > be easy to remove the chromogens as well. It would also be very easy to do > this with fluorescent tags too. Just remove the coverslip and dip the slide > in ethanol and it will remove the colored end product. Then just start over > for the new antigen. > > Good luck, > Amos > > On Fri, Oct 28, 2011 at 11:50 AM, > wrote: > > > Message: 2 > > Date: Thu, 27 Oct 2011 10:30:40 -0700 > > From: "Claire Weston" > > Subject: [Histonet] Stripping antibodies > > To: > > Message-ID: <002401cc94ce$2619ca60$724d5f20$@com> > > Content-Type: text/plain; charset="us-ascii" > > > > Hi! > > > > > > > > Does anyone have experience in IHC stripping antibodies from a tissue > > section and re-probing with different antibodies? What is the best way to > > do that? If you could share your stripping protocol or experience I would > > appreciate it - thanks! > > > > > > > > Claire > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > On 29/10/11, Amos Brooks wrote: > > Hi, > Stripping sites are usually found in the more seedy areas of large > cities ... Oh wait you meant ... nevermind :-) If you developed the reaction > with DAB, you may be in a difficult position. DAB is a really strong > reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in > 5% potassium premanganate for 5 min followed by 5% oxalic acid until it is > clear (usually a couple of minutes). Unfortunately, I am not sure what > effect this will have on the epitopes you are looking for, but this is > usually what is used to clean precipitated DAB from instruments it should > work on the tissue too. > If you use another chromogen such as AEC it is much easier (to many > people's dismay) to remove this end product. Using alkaline phosphatase will > be easy to remove the chromogens as well. It would also be very easy to do > this with fluorescent tags too. Just remove the coverslip and dip the slide > in ethanol and it will remove the colored end product. Then just start over > for the new antigen. > > Good luck, > Amos > > On Fri, Oct 28, 2011 at 11:50 AM, > wrote: > > > Message: 2 > > Date: Thu, 27 Oct 2011 10:30:40 -0700 > > From: "Claire Weston" > > Subject: [Histonet] Stripping antibodies > > To: > > Message-ID: <002401cc94ce$2619ca60$724d5f20$@com> > > Content-Type: text/plain; charset="us-ascii" > > > > Hi! > > > > > > > > Does anyone have experience in IHC stripping antibodies from a tissue > > section and re-probing with different antibodies? What is the best way to > > do that? If you could share your stripping protocol or experience I would > > appreciate it - thanks! > > > > > > > > Claire > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From congxf20002 <@t> hotmail.com Sun Oct 30 13:21:03 2011 From: congxf20002 <@t> hotmail.com (=?gb2312?B?tNTk7LfJ?=) Date: Sun Oct 30 13:21:08 2011 Subject: [Histonet] For zebrafish larvae section Message-ID: Hi, My respects! I wanted to do some zebrafish larvae sectioning. But some slice I made showed that the tissue inside had been broken. As to me, I thought the problem might be in dehydration time and and the razor I used. So can you please give me some advice to improve the process? In addition, I wanted to make some cross section. But it was difficult to me to control the orientation of the larvae. Thanks a lot! Best,Joseph Cong From cweston <@t> valasciences.com Sun Oct 30 13:58:19 2011 From: cweston <@t> valasciences.com (Claire Weston) Date: Sun Oct 30 13:58:30 2011 Subject: [Histonet] Stripping antibodies Message-ID: <20111030115819.1ECE91E5@resin12.mta.everyone.net>
Thank you everyone for your advice, I really appreciate it!  I am doing a multiplex immunofluorescent assay (4 channels) and I am planning to strip the antibodies and reprobe with a second round of antibodies.  The antibodies are labelled with quantum dots so I am not sure how that will influence things, but luckily I won't have the DAB problem several of you mentioned.  I think I will try several of these techniques and evaluate which works the best.
 
Thanks again for all your help,
 
Claire 

--- jkiernan@uwo.ca wrote:

From: John Kiernan <jkiernan@uwo.ca>
To: histonet@lists.utsouthwestern.edu, cweston@valasciences.com
Cc: Amos Brooks <amosbrooks@gmail.com>
Subject: Re: [Histonet] Stripping antibodies
Date: Sun, 30 Oct 2011 12:09:58 -0400

 
Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow.  If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents.
 
Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want?  This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody.
 
Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars).  The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios,  1999, is very good.
 
John Kiernan
Anatomy, UWO 
London, Canada 
= = = =
On 29/10/11, Amos Brooks <amosbrooks@gmail.com> wrote:
Hi,
    Stripping sites are usually found in the more seedy areas of large
cities ... Oh wait you meant ... nevermind :-) If you developed the reaction
with DAB, you may be in a difficult position. DAB is a really strong
reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in
5% potassium premanganate for 5 min followed by 5% oxalic acid until it is
clear (usually a couple of minutes). Unfortunately, I am not sure what
effect this will have on the epitopes you are looking for, but this is
usually what is used to clean precipitated DAB from instruments it should
work on the tissue too.
    If you use another chromogen such as AEC it is much easier (to many
people's dismay) to remove this end product. Using alkaline phosphatase will
be easy to remove the chromogens as well. It would also be very easy to do
this with fluorescent tags too. Just remove the coverslip and dip the slide
in ethanol and it will remove the colored end product. Then just start over
for the new antigen.

Good luck,
Amos

On Fri, Oct 28, 2011 at 11:50 AM, <histonet-request@lists.utsouthwestern.edu
> wrote:

> Message: 2
> Date: Thu, 27 Oct 2011 10:30:40 -0700
> From: "Claire Weston" <cweston@valasciences.com>
> Subject: [Histonet] Stripping antibodies
> To: <histonet@lists.utsouthwestern.edu>
> Message-ID: <002401cc94ce$2619ca60$724d5f20$@com>
> Content-Type: text/plain;       charset="us-ascii"
>
> Hi!
>
>
>
> Does anyone have experience in IHC stripping antibodies from a tissue
> section and re-probing with different antibodies?  What is the best way to
> do that?  If you could share your stripping protocol or experience I would
> appreciate it - thanks!
>
>
>
> Claire
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 
 
On 29/10/11, Amos Brooks <amosbrooks@gmail.com> wrote:
Hi,
    Stripping sites are usually found in the more seedy areas of large
cities ... Oh wait you meant ... nevermind :-) If you developed the reaction
with DAB, you may be in a difficult position. DAB is a really strong
reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in
5% potassium premanganate for 5 min followed by 5% oxalic acid until it is
clear (usually a couple of minutes). Unfortunately, I am not sure what
effect this will have on the epitopes you are looking for, but this is
usually what is used to clean precipitated DAB from instruments it should
work on the tissue too.
    If you use another chromogen such as AEC it is much easier (to many
people's dismay) to remove this end product. Using alkaline phosphatase will
be easy to remove the chromogens as well. It would also be very easy to do
this with fluorescent tags too. Just remove the coverslip and dip the slide
in ethanol and it will remove the colored end product. Then just start over
for the new antigen.

Good luck,
Amos

On Fri, Oct 28, 2011 at 11:50 AM, <histonet-request@lists.utsouthwestern.edu
> wrote:

> Message: 2
> Date: Thu, 27 Oct 2011 10:30:40 -0700
> From: "Claire Weston" <cweston@valasciences.com>
> Subject: [Histonet] Stripping antibodies
> To: <histonet@lists.utsouthwestern.edu>
> Message-ID: <002401cc94ce$2619ca60$724d5f20$@com>
> Content-Type: text/plain;       charset="us-ascii"
>
> Hi!
>
>
>
> Does anyone have experience in IHC stripping antibodies from a tissue
> section and re-probing with different antibodies?  What is the best way to
> do that?  If you could share your stripping protocol or experience I would
> appreciate it - thanks!
>
>
>
> Claire
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
From jkiernan <@t> uwo.ca Mon Oct 31 01:12:52 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Oct 31 01:13:04 2011 Subject: [Histonet] Stripping antibodies In-Reply-To: <20111030115819.1ECE91E5@resin12.mta.everyone.net> References: <20111030115819.1ECE91E5@resin12.mta.everyone.net> Message-ID: <7690e6ad626d2.4eae0424@uwo.ca> With quantum dots and a modern fluorescence microscope with "channels" it should be possible to label 2 or 3 antigens simultaneously, if you have all the right primaries and labelled secondaries. The company that sells you the quantum dot labelled secondaries or other amplification reagents should be in a position to tell you exactly what to do. Do they refund your hard-earned grant money if their expensive product fails to perform as advertized? Immunohistochemistry with non-fluorescent colours (available in brown, black, red and various blues) has advantages: (a) You do not need a fluorescence microscope. Modern fluorescence microscopes are very expensive; the old ones from the 1960s are no longer be good enough to provide publication-quality pictures. (b) Immunostained slides can be kept for many years in boxes at room temperature. (c) The colours do not fade in permanently mounted preparations. (c) You are not bound to use a commercially sold kit, which may not be optimized for your research. If you go by the book (= any book with references that you can follow up), you will do your multiple immunostains intelligently. Never follow a set of instructions without knowing or questioning the reason for ever step. John Kiernan Anatomy, UWO London, Canada. = = = On 30/10/11, Claire Weston wrote: > > > > Thank you everyone for your advice, I really appreciate it! I am doing a multiplex immunofluorescent assay (4 channels) and I am planning to strip the antibodies and reprobe with a second round of antibodies. The antibodies are labelled with quantum dots so I am not sure how that will influence things, but luckily I won't have the DAB problem several of you mentioned. I think I will try several of these techniques and evaluate which works the best. > > Thanks again for all your help, > > Claire > > --- jkiernan@uwo.ca wrote: > > From: John Kiernan > To: histonet@lists.utsouthwestern.edu, cweston@valasciences.com > Cc: Amos Brooks > Subject: Re: [Histonet] Stripping antibodies > Date: Sun, 30 Oct 2011 12:09:58 -0400 > > > > Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow. If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents. > > Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want? This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody. > > Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars). The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios, 1999, is very good. > > John Kiernan > Anatomy, UWO > London, Canada > = = = = > On 29/10/11, Amos Brooks wrote: > > > > > Hi, > > Stripping sites are usually found in the more seedy areas of large > > cities ... Oh wait you meant ... nevermind :-) If you developed the reaction > > with DAB, you may be in a difficult position. DAB is a really strong > > reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in > > 5% potassium premanganate for 5 min followed by 5% oxalic acid until it is > > clear (usually a couple of minutes). Unfortunately, I am not sure what > > effect this will have on the epitopes you are looking for, but this is > > usually what is used to clean precipitated DAB from instruments it should > > work on the tissue too. > > If you use another chromogen such as AEC it is much easier (to many > > people's dismay) to remove this end product. Using alkaline phosphatase will > > be easy to remove the chromogens as well. It would also be very easy to do > > this with fluorescent tags too. Just remove the coverslip and dip the slide > > in ethanol and it will remove the colored end product. Then just start over > > for the new antigen. > > > > Good luck, > > Amos > > > > On Fri, Oct 28, 2011 at 11:50 AM, > > wrote: > > > > > Message: 2 > > > Date: Thu, 27 Oct 2011 10:30:40 -0700 > > > From: "Claire Weston" > > > Subject: [Histonet] Stripping antibodies > > > To: > > > Message-ID: <002401cc94ce$2619ca60$724d5f20$@com> > > > Content-Type: text/plain; charset="us-ascii" > > > > > > Hi! > > > > > > > > > > > > Does anyone have experience in IHC stripping antibodies from a tissue > > > section and re-probing with different antibodies? What is the best way to > > > do that? If you could share your stripping protocol or experience I would > > > appreciate it - thanks! > > > > > > > > > > > > Claire > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > On 29/10/11, Amos Brooks wrote: > > > > > Hi, > > Stripping sites are usually found in the more seedy areas of large > > cities ... Oh wait you meant ... nevermind :-) If you developed the reaction > > with DAB, you may be in a difficult position. DAB is a really strong > > reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in > > 5% potassium premanganate for 5 min followed by 5% oxalic acid until it is > > clear (usually a couple of minutes). Unfortunately, I am not sure what > > effect this will have on the epitopes you are looking for, but this is > > usually what is used to clean precipitated DAB from instruments it should > > work on the tissue too. > > If you use another chromogen such as AEC it is much easier (to many > > people's dismay) to remove this end product. Using alkaline phosphatase will > > be easy to remove the chromogens as well. It would also be very easy to do > > this with fluorescent tags too. Just remove the coverslip and dip the slide > > in ethanol and it will remove the colored end product. Then just start over > > for the new antigen. > > > > Good luck, > > Amos > > > > On Fri, Oct 28, 2011 at 11:50 AM, > > wrote: > > > > > Message: 2 > > > Date: Thu, 27 Oct 2011 10:30:40 -0700 > > > From: "Claire Weston" > > > Subject: [Histonet] Stripping antibodies > > > To: > > > Message-ID: <002401cc94ce$2619ca60$724d5f20$@com> > > > Content-Type: text/plain; charset="us-ascii" > > > > > > Hi! > > > > > > > > > > > > Does anyone have experience in IHC stripping antibodies from a tissue > > > section and re-probing with different antibodies? What is the best way to > > > do that? If you could share your stripping protocol or experience I would > > > appreciate it - thanks! > > > > > > > > > > > > Claire > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > From NHeath <@t> Lifespan.org Mon Oct 31 05:20:57 2011 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Mon Oct 31 05:21:00 2011 Subject: [Histonet] SALARY In-Reply-To: <4EAAA586.7400.0077.1@harthosp.org> References: <91FD001DCC6DC84EA6A4B12BB9313678016ED690@uphmasphi007.UPHS.PENNHEALTH.PRV> <4EAAA586.7400.0077.1@harthosp.org> Message-ID: <130E8991F210424096EFC6F42EA33B240843564F@LSCOEXCH1.lsmaster.lifespan.org> "Histotechnologists are the most valuable employees in the laboratory today!" THANK YOU DR. CARTUN!! Nancy Heath, HT (ASCP) Neuropathology Technician Neuropathology Department Rhode Island Hospital President - Rhode Island Society for Histotechnology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 28, 2011 12:52 PM To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Caroline Pratt Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NHeath <@t> Lifespan.org Mon Oct 31 05:29:12 2011 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Mon Oct 31 05:29:15 2011 Subject: [Histonet] SALARY In-Reply-To: <5A605CE38EECB64B94485C02125A0C4405C741AF53@LN-MAIL07.ln.burnham.org> References: <91FD001DCC6DC84EA6A4B12BB9313678016ED690@uphmasphi007.UPHS.PENNHEALTH.PRV><4EAAA586.7400.0077.1@harthosp.org><91FD001DCC6DC84EA6A4B12BB9313678016ED6AB@uphmasphi007.UPHS.PENNHEALTH.PRV> <5A605CE38EECB64B94485C02125A0C4405C741AF53@LN-MAIL07.ln.burnham.org> Message-ID: <130E8991F210424096EFC6F42EA33B2408435651@LSCOEXCH1.lsmaster.lifespan.org> Just got offered a lead tech position, salary of $85600.00 in a private lab opened by a group of docs. Didn't take it because the health insurance plan they offered was lousey. 75% health covered with a 250.00 deductable, dental covered at 60% and no coverage for family or husband....would of had to pay that out of pocket. I'm in New England. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Friday, October 28, 2011 2:25 PM To: Pratt, Caroline; Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY With all that said what are your going rates in this area that you are speaking. Kind Regards! ? John J Shelley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Friday, October 28, 2011 2:19 PM To: Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY If that is an option, I would agree, but non-profit teaching hospitals have compensation ranges and grids according to education and experience. We keep every employee with the same education and skill set at the same compensation for an equitable pay system. If we feel our employees are being under paid we will conduct a market analysis and the rates will be increased for all applicable employees if the market analysis justifies it. Car :) -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Friday, October 28, 2011 12:52 PM To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, Caroline Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------------------------------------------------ - This message was secured by ZixCorp(R). The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NHeath <@t> Lifespan.org Mon Oct 31 05:30:04 2011 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Mon Oct 31 05:30:11 2011 Subject: [Histonet] SALARY In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DFB7C@nmdamailsvr.nmda.ad.nmsu.edu> References: <91FD001DCC6DC84EA6A4B12BB9313678016ED690@uphmasphi007.UPHS.PENNHEALTH.PRV><4EAAA586.7400.0077.1@harthosp.org><38667E7FB77ECD4E91BFAEB8D986386323E5E7D251@LRGHEXVS1.practice.lrgh.org> <4D14F0FC9316DD41972D5F03C070908B051DFB7C@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <130E8991F210424096EFC6F42EA33B2408435652@LSCOEXCH1.lsmaster.lifespan.org> I agree :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, October 28, 2011 3:22 PM To: Podawiltz, Thomas; Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Caroline Pratt Subject: RE: [Histonet] SALARY I nominate Dr. Cartun as the Patron Saint for Histopathology. We should work on a statue and a Mission Statement for him... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Mon Oct 31 07:33:01 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Oct 31 07:33:09 2011 Subject: [Histonet] Must be Monday... Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFB81@nmdamailsvr.nmda.ad.nmsu.edu> Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From lblazek <@t> digestivespecialists.com Mon Oct 31 07:40:02 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Oct 31 07:40:08 2011 Subject: [Histonet] RE: Must be Monday... In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DFB81@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B051DFB81@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E39127401B441@IBMB7Exchange.digestivespecialists.com> That's a hoot Sally! I don't think that DST of old was even this past weekend. For the past 3 years my processor changed on the DST of old and the powers that be told me it was impossible because that wasn't programmed into my processor's data. This year I forgot about it and it didn't change on the old date. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 8:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisls.com Mon Oct 31 08:01:31 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Mon Oct 31 08:01:46 2011 Subject: [Histonet] Florida Medical Directorship Message-ID: <6F33D8418806044682A391273399860F0A5B2974@s-irv-ex301.PathologyPartners.intranet> What are the true regulations' in Florida as far as time required for a Medical Director to be on site at the lab ? I am finding conflicting information and would like to know what is truly required. Thanks Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From igor.deyneko <@t> gmail.com Mon Oct 31 08:56:10 2011 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Mon Oct 31 08:56:16 2011 Subject: [Histonet] Problems with Frozen tissues Message-ID: I'm looking for some advice on frozen tissues. This is the first time I'm doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut well onto special Gold Plus slides from Fisher. Then, when I was ready to stain the slides, i air dried them fro an hour and wanted to do H&E and Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on preventing this mischief? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA From c.m.vanderloos <@t> amc.uva.nl Mon Oct 31 09:02:04 2011 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Mon Oct 31 09:02:17 2011 Subject: [Histonet] Stripping antibodies Message-ID: Amos and John, I do not agree with John saying that "antibodies could be easily removed by acidity pH1-2". High affinity primaries will stay at the tissue section nicely bound to the epitope. Try for example SMA, clone 1A4 antibody in the usual dilution. After this incubation strip off the antibody by glycin-HCl buffer pH2 and then apply an anti-mouse detection system. You will stain SMA for sure. The only way to get antibodies off is 10 min boiling with citrate pH6.0 or so, as in HIER. I have described that in JOH in 2008, vol 31, pp. 119-127, being the basis of an AP sequential double staining. Chris van der Loos Academic Medical Center Dept. of Pathology M2-230 Amsterdam The Netherlands Date: Sun, 30 Oct 2011 12:09:58 -0400 From: John Kiernan Subject: Re: [Histonet] Stripping antibodies To: histonet@lists.utsouthwestern.edu, cweston@valasciences.com Cc: Amos Brooks Message-ID: <7640acec48487.4ead3e96@uwo.ca> Content-Type: text/plain; CHARSET=US-ASCII Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow. If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents. Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want? This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody. Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars). The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios, 1999, is very good. John Kiernan Anatomy, UWO London, Canada From SLB <@t> stowers.org Mon Oct 31 09:22:38 2011 From: SLB <@t> stowers.org (Beckham, Sharon) Date: Mon Oct 31 09:23:12 2011 Subject: [Histonet] RE: Must be Monday... In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39127401B441@IBMB7Exchange.digestivespecialists.com> References: <4D14F0FC9316DD41972D5F03C070908B051DFB81@nmdamailsvr.nmda.ad.nmsu.edu> <5A2BD13465E061429D6455C8D6B40E39127401B441@IBMB7Exchange.digestivespecialists.com> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF98AEE2C42@EXCHMB-02.stowers-institute.org> Yes it was this past weekend back when. My alarm clock went to DST Sunday morning. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, October 31, 2011 7:40 AM To: 'Breeden, Sara'; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Must be Monday... That's a hoot Sally! I don't think that DST of old was even this past weekend. For the past 3 years my processor changed on the DST of old and the powers that be told me it was impossible because that wasn't programmed into my processor's data. This year I forgot about it and it didn't change on the old date. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 8:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Mon Oct 31 09:25:58 2011 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Mon Oct 31 09:26:05 2011 Subject: [Histonet] RE: SALARY In-Reply-To: <1da65ac5-651d-42ee-a1ea-c9ed9f641b61@DCPWPRTR04.mdanderson.edu> References: <1da65ac5-651d-42ee-a1ea-c9ed9f641b61@DCPWPRTR04.mdanderson.edu> Message-ID: Add location to that. $28-32 is great for places that do not have an abundant supply of techs. Here in Houston, we have 2 schools and a very large medical center. Entry level techs are not hard to find. Graduates expect $23-28 minimum depending on facility. In other states, neighboring Louisiana and such, they expect $21-24. It just depends on the cost of living in the area. Entry supervisors earn $30+ an hour, but again, it is based on cost of living and work conditions. Reference labs pay more, but expect more. My first supervisor job paid $32, and I worked like crazy. It wasn't worth it. You get what you pay for though, and in this economic climate, the high salaries are not as plentiful as they once were. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Message: 6 Date: Fri, 28 Oct 2011 12:52:23 -0400 From: "Richard Cartun" Subject: RE: [Histonet] SALARY To: ,, "Caroline Pratt" Message-ID: <4EAAA586.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 95, Issue 34 **************************************** From joelleweaver <@t> hotmail.com Mon Oct 31 09:35:21 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Oct 31 09:35:26 2011 Subject: [Histonet] SALARY In-Reply-To: <130E8991F210424096EFC6F42EA33B2408435651@LSCOEXCH1.lsmaster.lifespan.org> References: <91FD001DCC6DC84EA6A4B12BB9313678016ED690@uphmasphi007.UPHS.PENNHEALTH.PRV><4EAAA586.7400.0077.1@harthosp.org><91FD001DCC6DC84EA6A4B12BB9313678016ED6AB@uphmasphi007.UPHS.PENNHEALTH.PRV>, <5A605CE38EECB64B94485C02125A0C4405C741AF53@LN-MAIL07.ln.burnham.org>, <130E8991F210424096EFC6F42EA33B2408435651@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: I know the cost of living is greater on the east coast, but that sounds good to me. The insurance is not better, and as an HTL I make less than 1/2 of that! Things are determined by the market I realize, but all these postings are sure making me feel bad and also realize that I am not marketing myself well.... Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Mon, 31 Oct 2011 06:29:12 -0400 > From: NHeath@Lifespan.org > To: jshelley@sanfordburnham.org; Caroline.Pratt@uphs.upenn.edu; Rcartun@harthosp.org; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] SALARY > CC: > > Just got offered a lead tech position, salary of $85600.00 in a private lab opened by a group of docs. Didn't take it because the health insurance plan they offered was lousey. 75% health covered with a 250.00 deductable, dental covered at 60% and no coverage for family or husband....would of had to pay that out of pocket. > I'm in New England. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley > Sent: Friday, October 28, 2011 2:25 PM > To: Pratt, Caroline; Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] SALARY > > With all that said what are your going rates in this area that you are speaking. > > Kind Regards! > > John J Shelley > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline > Sent: Friday, October 28, 2011 2:19 PM > To: Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] SALARY > > If that is an option, I would agree, but non-profit teaching hospitals > have compensation ranges and grids according to education and > experience. We keep every employee with the same education and skill > set at the same compensation for an equitable pay system. If we feel > our employees are being under paid we will conduct a market analysis and > the rates will be increased for all applicable employees if the market > analysis justifies it. > > Car :) > > -----Original Message----- > From: Richard Cartun [mailto:Rcartun@harthosp.org] > Sent: Friday, October 28, 2011 12:52 PM > To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, > Caroline > Subject: RE: [Histonet] SALARY > > I think that's low. If you find a good candidate with years of > experience I would pay them whatever it takes to get them in the door. > It's like "Free Agency" in baseball; if you want a good player, you need > to put the "money on table". Histotechnologists are the most valuable > employees in the laboratory today! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> "Pratt, Caroline" 10/28/2011 9:21 AM > >>> > I would say $28 to $32 dollars an hour depending on experience and > education. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > kcastillo@frii.com > Sent: Friday, October 28, 2011 7:54 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] SALARY > > HI EVERYONE, > > WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID > THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM > LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this e-mail message is intended only for > the personal and confidential use of the recipient(s) named above. If > the reader of this message is not the intended recipient or an agent > responsible for delivering it to the intended recipient, you are hereby > notified that you have received this document in error and that any > review, dissemination, distribution, or copying of this message is > strictly prohibited. If you have received this communication in error, > please notify us immediately by e-mail, and delete the original message. > > > > > > ------------------------------------------------------------------------ > - > This message was secured by ZixCorp(R). > > > The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Mon Oct 31 09:36:26 2011 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Oct 31 09:36:30 2011 Subject: [Histonet] RE: need help with TMA In-Reply-To: References: Message-ID: Hello, Do 30 minutes at 37 degrees on a glass slide. take from oven and apply gentle pressure to center of block and slide complex, cool to RT and repeat 1 or two times. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia F Lott Sent: Friday, October 28, 2011 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] need help with TMA We have recently started doing tissue microarrays. The problem we have is that some of the small cores drop out after the first few sections. I think my problem is in the step where we are supposed to melt the donor and recipient blocks together. We tried 5 minutes at 37 degrees, and then minutes at 60 degrees x3, with cooling to room temp in between. Any suggestions? Thanks, Patty Lott, UAB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Oct 31 09:40:13 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Oct 31 09:40:17 2011 Subject: [Histonet] RE: SALARY In-Reply-To: References: <1da65ac5-651d-42ee-a1ea-c9ed9f641b61@DCPWPRTR04.mdanderson.edu>, Message-ID: Still sounds good to me, at my first supervisor's job ( the credentials I had at the time were HTL + Bachelor's), and I made less than $23 per hour and worked an average of 16 hours a day with no OT or comp time that was ever granted ( though promised). I guess I am just trying to say, count your blessings. Having a school near by really seems to impact the supply and demand and the general hiring environment. What I have noticed also is that when you go somewhere and the cost of living is less the tax burden is greater, so it has always seemed to come "out in the wash". Anyhow, all these salary revelations have really made me think, thanks for the postings. Joelle Weaver MAOM, BA, (HTL) ASCP > From: TNMayer@mdanderson.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 31 Oct 2011 09:25:58 -0500 > Subject: [Histonet] RE: SALARY > > Add location to that. $28-32 is great for places that do not have an abundant supply of techs. Here in Houston, we have 2 schools and a very large medical center. Entry level techs are not hard to find. Graduates expect $23-28 minimum depending on facility. > In other states, neighboring Louisiana and such, they expect $21-24. It just depends on the cost of living in the area. > Entry supervisors earn $30+ an hour, but again, it is based on cost of living and work conditions. Reference labs pay more, but expect more. > > My first supervisor job paid $32, and I worked like crazy. It wasn't worth it. > You get what you pay for though, and in this economic climate, the high salaries are not as plentiful as they once were. > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > Message: 6 > Date: Fri, 28 Oct 2011 12:52:23 -0400 > From: "Richard Cartun" > Subject: RE: [Histonet] SALARY > To: ,, > "Caroline Pratt" > Message-ID: <4EAAA586.7400.0077.1@harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> > I would say $28 to $32 dollars an hour depending on experience and education. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com > Sent: Friday, October 28, 2011 7:54 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] SALARY > > HI EVERYONE, > > WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID > THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM > LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 95, Issue 34 > **************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicole <@t> dlcjax.com Mon Oct 31 09:33:12 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Mon Oct 31 09:45:41 2011 Subject: [Histonet] background checks required by AHCA Message-ID: <1533.208.62.167.196.1320071592.squirrel@webmail.realpages.com> I am currently filing out my ahca renewal application. There are many changes and the form and I do not find it user friendly. Anyways here my question: Medical directors and chief finicial officers or any person who stands to gain profit must undergo a level 2 background screeening. On the new app is states all empolyees and health care providers. I called acha licensing division and they said only directors and so forth. But, on ahca website statue 408(something, ill have to get exact number) when into effect in 2010 that says all employees and newly hired employees must underground background screening. So does all the arnp, pa, ht, and ma's need to be screened because we have a lab? Nicole Tatum, HT ASCP http://ahca.myflorida.com/mchq/long_term_care/Background_Screening/BGS_WhoRequiredToBeScreened.pdf taken directly from ahca site: Employees and Contractors employed before August 1, 2010 Every employee/contractor must attest to meeting the requirements of this chapter and agreeing to inform the employer immediately if arrested for any of the disqualifying offenses while employed by the employer. [Section 435.05(2)]. This attestation must be maintained in the employee?s personnel file. You may use the Affidavit of Compliance with Background Screening to satisfy the attestation requirement. If an employer becomes aware that an employee/contractor has been arrested for a disqualifying offense, the employer must remove the employee/contractor from contact with any vulnerable person that places the employee/contractor in a role that requires background screening until the arrest is resolved in a way that the employer determines that the employee/contractor is still eligible for employment/contracting under this chapter. [Section 435.06(2)(b)] Rescreening From ROrr <@t> northshore.org Mon Oct 31 10:56:17 2011 From: ROrr <@t> northshore.org (Orr, Rebecca) Date: Mon Oct 31 10:56:23 2011 Subject: [Histonet] Dako Pr on Ventana? In-Reply-To: References: Message-ID: Hi Friends, If anyone is using Dako PR concentrate clone 1294 on a Ventana, could you please contact me separately? I'd like some advice. Thank you Becky Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 -----Original Message----- Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. From one_angel_secret <@t> yahoo.com Mon Oct 31 11:01:51 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Oct 31 11:01:57 2011 Subject: [Histonet] Problems with Frozen tissues In-Reply-To: References: Message-ID: <1320076911.80808.YahooMailNeo@web112303.mail.gq1.yahoo.com> Ive never just air dried my frozen sections. always put them in a fixative such as pen fix, a alcohol and or formalin mixture, something( depends on what your going to look for, test etc). That with using charged slides and never had too many problems with this. ? Kim ________________________________ From: Igor Deyneko To: Histonet@lists.utsouthwestern.edu Sent: Monday, October 31, 2011 9:56 AM Subject: [Histonet] Problems with Frozen tissues I'm looking for some advice on frozen tissues. This is the first time I'm doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut well onto special Gold Plus slides from Fisher. Then, when I was ready to stain the slides, i air dried them fro an hour and wanted to do H&E and Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on preventing this mischief? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSetlak <@t> childrensmemorial.org Mon Oct 31 11:09:21 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Mon Oct 31 11:09:27 2011 Subject: [Histonet] slide labeling In-Reply-To: References: Message-ID: <7111DB39D045004C9CF29E79C71B28BC109F809657@CMHEXCC01MBX.childrensmemorial.org> Hi, Just curious because this questions has come up multiple times from non-histology people so I thought I'd post it here. When labeling slides at microtomy what info do you include on the slide? I know the automated slide labelers that are out there will include patient name, surgical number etc.; but when handwriting slides as you cut what do you write on them? Currently we write the case number, part number and level as well as the cutters initials. After staining we put the permanent label on that has patient name and case number etc (hence two identifiers). Is there anyone out there who hand writes the patient name on the slide or a second identifier on it at the time of microtomy? Thanks for your help, Lisa Lisa V. Chg., IL From making <@t> ufl.edu Mon Oct 31 11:19:12 2011 From: making <@t> ufl.edu (MKing) Date: Mon Oct 31 11:15:31 2011 Subject: [Histonet] stripping antibodies Message-ID: <4EAECA80.7060802@ufl.edu> If your antibodies are labeled with quantum dots and you strip the antibodies (e.g. with low pH) you will no longer have labeled targets in your tissue. Not clear if you understood this from your post. Message: 2 Date: Sun, 30 Oct 2011 11:58:19 -0700 From: "Claire Weston" Subject: Re: [Histonet] Stripping antibodies From TGoins <@t> mt.gov Mon Oct 31 11:20:07 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Mon Oct 31 11:20:13 2011 Subject: [Histonet] RE: slide labeling In-Reply-To: <7111DB39D045004C9CF29E79C71B28BC109F809657@CMHEXCC01MBX.childrensmemorial.org> References: <7111DB39D045004C9CF29E79C71B28BC109F809657@CMHEXCC01MBX.childrensmemorial.org> Message-ID: Hi Lisa - We hand write our labels, but do so prior to cutting sections. The accession numbers [unique to every slide] are read from a printed data sheet and written on the slides. This allows verification that the numbers match [slide and block] as the tissues are cut. If the numbers are written at microtomy I see it as a source of possible labeling errors. All blocks and slides are processed in numerical order so there is a number check at embedding, at cutting and at final labeling. Tresa Goins Veterinary Diagnostic Lab South 19th and Lincoln Bozeman, MT 59718 406-994-6353 - phone 406-994-6344 - fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa Sent: Monday, October 31, 2011 10:09 AM To: 'histonet@lists.utsouthwestern.edu'; 'histonet-request@lists.utsouthwestern.edu'; 'histonet-bounces@lists.utsouthwestern.edu' Subject: [Histonet] slide labeling Hi, Just curious because this questions has come up multiple times from non-histology people so I thought I'd post it here. When labeling slides at microtomy what info do you include on the slide? I know the automated slide labelers that are out there will include patient name, surgical number etc.; but when handwriting slides as you cut what do you write on them? Currently we write the case number, part number and level as well as the cutters initials. After staining we put the permanent label on that has patient name and case number etc (hence two identifiers). Is there anyone out there who hand writes the patient name on the slide or a second identifier on it at the time of microtomy? Thanks for your help, Lisa Lisa V. Chg., IL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Mon Oct 31 11:20:15 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Oct 31 11:20:19 2011 Subject: [Histonet] Re: Stripping antibodies In-Reply-To: References: Message-ID: <76a08af94b352.4eae927f@uwo.ca> Thanks Chris. I didn't know there were antibodies with such strong adhesion. John. = = = On 31/10/11, "C.M. van der Loos" wrote: > > Amos and John, > I do not agree with John saying that "antibodies could be easily removed by acidity pH1-2". High affinity primaries will stay at the tissue section nicely bound to the epitope. Try for example SMA, clone 1A4 antibody in the usual dilution. After this incubation strip off the antibody by glycin-HCl buffer pH2 and then apply an anti-mouse detection system. You will stain SMA for sure. The only way to get antibodies off is 10 min boiling with citrate pH6.0 or so, as in HIER. I have described that in JOH in 2008, vol 31, pp. 119-127, being the basis of an AP sequential double staining. > > Chris van der Loos > Academic Medical Center > Dept. of Pathology M2-230 > Amsterdam > The Netherlands > > Date: Sun, 30 Oct 2011 12:09:58 -0400 > From: John Kiernan > Subject: Re: [Histonet] Stripping antibodies > To: histonet@lists.utsouthwestern.edu, cweston@valasciences.com > Cc: Amos Brooks > Message-ID: <7640acec48487.4ead3e96@uwo.ca> > Content-Type: text/plain; CHARSET=US-ASCII > > > Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow. If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents. > > Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want? This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody. > > Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars). The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios, 1999, is very good. > > John Kiernan > Anatomy, UWO > London, Canada > > From LSebree <@t> uwhealth.org Mon Oct 31 11:52:23 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Oct 31 11:52:27 2011 Subject: [Histonet] background checks required by AHCA In-Reply-To: <1533.208.62.167.196.1320071592.squirrel@webmail.realpages.com> References: <1533.208.62.167.196.1320071592.squirrel@webmail.realpages.com> Message-ID: Everyone employed in our hospital undergoes periodic background checks. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Monday, October 31, 2011 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] background checks required by AHCA I am currently filing out my ahca renewal application. There are many changes and the form and I do not find it user friendly. Anyways here my question: Medical directors and chief finicial officers or any person who stands to gain profit must undergo a level 2 background screeening. On the new app is states all empolyees and health care providers. I called acha licensing division and they said only directors and so forth. But, on ahca website statue 408(something, ill have to get exact number) when into effect in 2010 that says all employees and newly hired employees must underground background screening. So does all the arnp, pa, ht, and ma's need to be screened because we have a lab? Nicole Tatum, HT ASCP http://ahca.myflorida.com/mchq/long_term_care/Background_Screening/BGS_W hoRequiredToBeScreened.pdf taken directly from ahca site: Employees and Contractors employed before August 1, 2010 Every employee/contractor must attest to meeting the requirements of this chapter and agreeing to inform the employer immediately if arrested for any of the disqualifying offenses while employed by the employer. [Section 435.05(2)]. This attestation must be maintained in the employee's personnel file. You may use the Affidavit of Compliance with Background Screening to satisfy the attestation requirement. If an employer becomes aware that an employee/contractor has been arrested for a disqualifying offense, the employer must remove the employee/contractor from contact with any vulnerable person that places the employee/contractor in a role that requires background screening until the arrest is resolved in a way that the employer determines that the employee/contractor is still eligible for employment/contracting under this chapter. [Section 435.06(2)(b)] Rescreening _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Mon Oct 31 11:56:33 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Mon Oct 31 11:56:38 2011 Subject: [Histonet] The SC Histo Meeting in Myrtle Beach, SC Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA2731F0ACEA@NADCWPMSGCMS03.hca.corpad.net> November 4-5, 2011 is the SC Society of Histotechnology meeting at Marina Inn at Grande Dunes in Myrtle Beach, SC. Everyone is still invited even if you have not pre-registered!!!!! Gayle Callis is presenting 2 workshops and we will have good food and good fellowship!!!! If you think you are coming, please email me back so we can get a head count!!! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From TJohnson <@t> gnf.org Mon Oct 31 12:04:43 2011 From: TJohnson <@t> gnf.org (Theresa (Teri) Johnson) Date: Mon Oct 31 12:04:47 2011 Subject: [Histonet] Automated coverslipper with Ventana labels Message-ID: <9F3CFEE76E51B64991C7485270890B4009ED52D1@EX5.lj.gnf.org> Hi all, I wanted to ask if any of you out there are currently using an automated coverslipper and do not have issues with the forks picking up the slides if they have Ventana labels on them. I have used a Leica and had only very occasionally a dropped slide. Is this your experience as well? Are there other units that would work well for this? Feel free to contact me off list if you need to. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From BDeBrosse-Serra <@t> isisph.com Mon Oct 31 12:25:34 2011 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Mon Oct 31 12:25:41 2011 Subject: [Histonet] RE: Automated coverslipper with Ventana labels In-Reply-To: <9F3CFEE76E51B64991C7485270890B4009ED52D1@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B4009ED52D1@EX5.lj.gnf.org> Message-ID: <493CAA64F203E14E8823737B9EE0E25F091E912750@EXCHMB01.isis.local> Hi Teri, And welcome to San Diego!!!!!!!! :-) My experience is, that you cannot leave the slides with the Ventana labels sitting in xylene for too long before coverslipping. I believe it is just how these labels are and doesn't have much to do with the coverslipper itself. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Theresa (Teri) Johnson Sent: Monday, October 31, 2011 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated coverslipper with Ventana labels Hi all, I wanted to ask if any of you out there are currently using an automated coverslipper and do not have issues with the forks picking up the slides if they have Ventana labels on them. I have used a Leica and had only very occasionally a dropped slide. Is this your experience as well? Are there other units that would work well for this? Feel free to contact me off list if you need to. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Mon Oct 31 12:34:14 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Oct 31 12:34:07 2011 Subject: [Histonet] RE: Automated coverslipper with Ventana labels In-Reply-To: <493CAA64F203E14E8823737B9EE0E25F091E912750@EXCHMB01.isis.local> References: <9F3CFEE76E51B64991C7485270890B4009ED52D1@EX5.lj.gnf.org> <493CAA64F203E14E8823737B9EE0E25F091E912750@EXCHMB01.isis.local> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F5F484@SMCMAIL01.somerset-healthcare.com> Although we do not have a Ventana stainer, we did demo one a few years back. At the time, there were no coverslipping problems with our Leica CV5030. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Monday, October 31, 2011 1:26 PM To: 'Theresa (Teri) Johnson'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated coverslipper with Ventana labels Hi Teri, And welcome to San Diego!!!!!!!! :-) My experience is, that you cannot leave the slides with the Ventana labels sitting in xylene for too long before coverslipping. I believe it is just how these labels are and doesn't have much to do with the coverslipper itself. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Theresa (Teri) Johnson Sent: Monday, October 31, 2011 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated coverslipper with Ventana labels Hi all, I wanted to ask if any of you out there are currently using an automated coverslipper and do not have issues with the forks picking up the slides if they have Ventana labels on them. I have used a Leica and had only very occasionally a dropped slide. Is this your experience as well? Are there other units that would work well for this? Feel free to contact me off list if you need to. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From mw <@t> personifysearch.com Mon Oct 31 12:48:55 2011 From: mw <@t> personifysearch.com (Matt Ward) Date: Mon Oct 31 12:51:03 2011 Subject: [Histonet] Field Histology Tech FL/GA/AL Message-ID: <85016ea9c74b06d58323135281586359@mail.gmail.com> Good Afternoon, New Position Alert! Our client is a world leader in Cancer Diagnostics who is currently seeking Histotechs who have experience working with IHC analyzers that would be interested in Field Support roles. We currently have an opening covering Northern FL, AL, and part of GA. The Position Offers: - Outstanding Base Salary and Competitive Bonus! - Gold Standard Benefits including but not limited to Medical, Cell Phone, Laptop, Car Allowance, Expenses, 401k, Paid Vacation! - Opportunity for Career Advancement! If you are interested in learning more please contact me directly at 800.875.6188 ext. 103 or mw@personifysearch.com Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From NHeath <@t> Lifespan.org Mon Oct 31 13:11:10 2011 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Mon Oct 31 13:11:21 2011 Subject: [Histonet] SALARY In-Reply-To: References: <91FD001DCC6DC84EA6A4B12BB9313678016ED690@uphmasphi007.UPHS.PENNHEALTH.PRV><4EAAA586.7400.0077.1@harthosp.org><91FD001DCC6DC84EA6A4B12BB9313678016ED6AB@uphmasphi007.UPHS.PENNHEALTH.PRV>, <5A605CE38EECB64B94485C02125A0C4405C741AF53@LN-MAIL07.ln.burnham.org>, <130E8991F210424096EFC6F42EA33B2408435651@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: <130E8991F210424096EFC6F42EA33B24084357BF@LSCOEXCH1.lsmaster.lifespan.org> Not so good when you fall into a higher tax bracket and uncle sam takes 25% of your salary and the insurance with dental weekly would have cost me around $232.55 a week...no thanks From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Monday, October 31, 2011 10:35 AM To: Heath, Nancy L.; jshelley@sanfordburnham.org; caroline.pratt@uphs.upenn.edu; rcartun@harthosp.org; kcastillo@frii.com; Histonet Subject: RE: [Histonet] SALARY I know the cost of living is greater on the east coast, but that sounds good to me. The insurance is not better, and as an HTL I make less than 1/2 of that! Things are determined by the market I realize, but all these postings are sure making me feel bad and also realize that I am not marketing myself well.... Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Mon, 31 Oct 2011 06:29:12 -0400 > From: NHeath@Lifespan.org > To: jshelley@sanfordburnham.org; Caroline.Pratt@uphs.upenn.edu; Rcartun@harthosp.org; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] SALARY > CC: > > Just got offered a lead tech position, salary of $85600.00 in a private lab opened by a group of docs. Didn't take it because the health insurance plan they offered was lousey. 75% health covered with a 250.00 deductable, dental covered at 60% and no coverage for family or husband....would of had to pay that out of pocket. > I'm in New England. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley > Sent: Friday, October 28, 2011 2:25 PM > To: Pratt, Caroline; Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] SALARY > > With all that said what are your going rates in this area that you are speaking. > > Kind Regards! > > John J Shelley > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline > Sent: Friday, October 28, 2011 2:19 PM > To: Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] SALARY > > If that is an option, I would agree, but non-profit teaching hospitals > have compensation ranges and grids according to education and > experience. We keep every employee with the same education and skill > set at the same compensation for an equitable pay system. If we feel > our employees are being under paid we will conduct a market analysis and > the rates will be increased for all applicable employees if the market > analysis justifies it. > > Car :) > > -----Original Message----- > From: Richard Cartun [mailto:Rcartun@harthosp.org] > Sent: Friday, October 28, 2011 12:52 PM > To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, > Caroline > Subject: RE: [Histonet] SALARY > > I think that's low. If you find a good candidate with years of > experience I would pay them whatever it takes to get them in the door. > It's like "Free Agency" in baseball; if you want a good player, you need > to put the "money on table". Histotechnologists are the most valuable > employees in the laboratory today! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> "Pratt, Caroline" 10/28/2011 9:21 AM > >>> > I would say $28 to $32 dollars an hour depending on experience and > education. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > kcastillo@frii.com > Sent: Friday, October 28, 2011 7:54 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] SALARY > > HI EVERYONE, > > WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID > THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM > LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this e-mail message is intended only for > the personal and confidential use of the recipient(s) named above. If > the reader of this message is not the intended recipient or an agent > responsible for delivering it to the intended recipient, you are hereby > notified that you have received this document in error and that any > review, dissemination, distribution, or copying of this message is > strictly prohibited. If you have received this communication in error, > please notify us immediately by e-mail, and delete the original message. > > > > > > ------------------------------------------------------------------------ > - > This message was secured by ZixCorp(R). > > > The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From azdudley <@t> hotmail.com Mon Oct 31 13:55:28 2011 From: azdudley <@t> hotmail.com (anita dudley) Date: Mon Oct 31 13:55:33 2011 Subject: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus In-Reply-To: References: Message-ID: allison, we do all of our alcian blue on the machine. we love it!! anita dudley providence hosp mobile, ala > From: Allison_Scott@hchd.tmc.edu > To: histonet@lists.utsouthwestern.edu > Date: Fri, 21 Oct 2011 14:00:44 +0000 > Subject: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus > > Hello to all in histo land. For those of you that have the ventana special stains machine, can the alcian blue 2.5 be done on the machine? I am not sure if they have kits for this stain. Also is anyone using the cryoembedding apparatus from pathology innovations? Thanks in advance. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital. > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erik.Dokken <@t> onassignment.com Mon Oct 31 15:11:05 2011 From: Erik.Dokken <@t> onassignment.com (Erik Dokken) Date: Mon Oct 31 15:11:14 2011 Subject: [Histonet] Contract to Hire Opportunity in SF Bay Area. In-Reply-To: <201110311600.p9VFui6o003119@smtp12.onasgn.net> References: <201110311600.p9VFui6o003119@smtp12.onasgn.net> Message-ID: We have an immediate full time opening for a qualified Histo Tech in the SF Bay Area. If you would like additional information please contact me directly. Erik Dokken Area Manager - No. CA, WA, and TX On Assignment, Inc. Local Allied Healthcare fax. 866-604-0193 NASDAQ: ASGN www.oahealthcare.com People First -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, October 31, 2011 9:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 95, Issue 37 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. For zebrafish larvae section (=?gb2312?B?tNTk7LfJ?=) 2. Re: Stripping antibodies (Claire Weston) 3. Re: Stripping antibodies (John Kiernan) 4. RE: SALARY (Heath, Nancy L.) 5. RE: SALARY (Heath, Nancy L.) 6. RE: SALARY (Heath, Nancy L.) 7. Must be Monday... (Breeden, Sara) 8. RE: Must be Monday... (Blazek, Linda) 9. Florida Medical Directorship (Hale, Meredith) 10. Problems with Frozen tissues (Igor Deyneko) 11. Stripping antibodies (C.M. van der Loos) 12. RE: Must be Monday... (Beckham, Sharon) 13. RE: SALARY (Mayer,Toysha N) 14. RE: SALARY (joelle weaver) 15. RE: need help with TMA (Helen Fedor) 16. RE: RE: SALARY (joelle weaver) 17. background checks required by AHCA (Nicole Tatum) 18. Dako Pr on Ventana? (Orr, Rebecca) ---------------------------------------------------------------------- Message: 1 Date: Mon, 31 Oct 2011 02:21:03 +0800 From: =?gb2312?B?tNTk7LfJ?= Subject: [Histonet] For zebrafish larvae section To: Message-ID: Content-Type: text/plain; charset="gb2312" Hi, My respects! I wanted to do some zebrafish larvae sectioning. But some slice I made showed that the tissue inside had been broken. As to me, I thought the problem might be in dehydration time and and the razor I used. So can you please give me some advice to improve the process? In addition, I wanted to make some cross section. But it was difficult to me to control the orientation of the larvae. Thanks a lot! Best,Joseph Cong ------------------------------ Message: 2 Date: Sun, 30 Oct 2011 11:58:19 -0700 From: "Claire Weston" Subject: Re: [Histonet] Stripping antibodies To: "John Kiernan" Cc: histonet@lists.utsouthwestern.edu, amosbrooks@gmail.com Message-ID: <20111030115819.1ECE91E5@resin12.mta.everyone.net> Content-Type: text/plain; charset="UTF-8"
Thank you everyone for your advice, I really appreciate it!  I am doing a multiplex immunofluorescent assay (4 channels) and I am planning to strip the antibodies and reprobe with a second round of antibodies.  The antibodies are labelled with quantum dots so I am not sure how that will influence things, but luckily I won't have the DAB problem several of you mentioned.  I think I will try several of these techniques and evaluate which works the best.
 
Thanks again for all your help,
 
Claire 

--- jkiernan@uwo.ca wrote:

From: John Kiernan <jkiernan@uwo.ca>
To: histonet@lists.utsouthwestern.edu, cweston@valasciences.com
Cc: Amos Brooks <amosbrooks@gmail.com>
Subject: Re: [Histonet] Stripping antibodies
Date: Sun, 30 Oct 2011 12:09:58 -0400

 
Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow.  If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents.
 
Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want?  This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody.
 
Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars).  The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios,  1999, is very good.
 
John Kiernan
Anatomy, UWO 
London, Canada 
= = = =
On 29/10/11, Amos Brooks <amosbrooks@gmail.com> wrote:
Hi,
    Stripping sites are usually found in the more seedy areas of large
cities ... Oh wait you meant ... nevermind :-) If you developed the reaction
with DAB, you may be in a difficult position. DAB is a really strong
reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in
5% potassium premanganate for 5 min followed by 5% oxalic acid until it is
clear (usually a couple of minutes). Unfortunately, I am not sure what
effect this will have on the epitopes you are looking for, but this is
usually what is used to clean precipitated DAB from instruments it should
work on the tissue too.
    If you use another chromogen such as AEC it is much easier (to many
people's dismay) to remove this end product. Using alkaline phosphatase will
be easy to remove the chromogens as well. It would also be very easy to do
this with fluorescent tags too. Just remove the coverslip and dip the slide
in ethanol and it will remove the colored end product. Then just start over
for the new antigen.

Good luck,
Amos

On Fri, Oct 28, 2011 at 11:50 AM, <histonet-request@lists.utsouthwestern.edu
> wrote:

> Message: 2
> Date: Thu, 27 Oct 2011 10:30:40 -0700
> From: "Claire Weston" <cweston@valasciences.com>
> Subject: [Histonet] Stripping antibodies
> To: <histonet@lists.utsouthwestern.edu>
> Message-ID: <002401cc94ce$2619ca60$724d5f20$@com>
> Content-Type: text/plain;       charset="us-ascii"
>
> Hi!
>
>
>
> Does anyone have experience in IHC stripping antibodies from a tissue
> section and re-probing with different antibodies?  What is the best way to
> do that?  If you could share your stripping protocol or experience I would
> appreciate it - thanks!
>
>
>
> Claire
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 
 
On 29/10/11, Amos Brooks <amosbrooks@gmail.com> wrote:
Hi,
    Stripping sites are usually found in the more seedy areas of large
cities ... Oh wait you meant ... nevermind :-) If you developed the reaction
with DAB, you may be in a difficult position. DAB is a really strong
reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in
5% potassium premanganate for 5 min followed by 5% oxalic acid until it is
clear (usually a couple of minutes). Unfortunately, I am not sure what
effect this will have on the epitopes you are looking for, but this is
usually what is used to clean precipitated DAB from instruments it should
work on the tissue too.
    If you use another chromogen such as AEC it is much easier (to many
people's dismay) to remove this end product. Using alkaline phosphatase will
be easy to remove the chromogens as well. It would also be very easy to do
this with fluorescent tags too. Just remove the coverslip and dip the slide
in ethanol and it will remove the colored end product. Then just start over
for the new antigen.

Good luck,
Amos

On Fri, Oct 28, 2011 at 11:50 AM, <histonet-request@lists.utsouthwestern.edu
> wrote:

> Message: 2
> Date: Thu, 27 Oct 2011 10:30:40 -0700
> From: "Claire Weston" <cweston@valasciences.com>
> Subject: [Histonet] Stripping antibodies
> To: <histonet@lists.utsouthwestern.edu>
> Message-ID: <002401cc94ce$2619ca60$724d5f20$@com>
> Content-Type: text/plain;       charset="us-ascii"
>
> Hi!
>
>
>
> Does anyone have experience in IHC stripping antibodies from a tissue
> section and re-probing with different antibodies?  What is the best way to
> do that?  If you could share your stripping protocol or experience I would
> appreciate it - thanks!
>
>
>
> Claire
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
------------------------------ Message: 3 Date: Mon, 31 Oct 2011 02:12:52 -0400 From: John Kiernan Subject: Re: [Histonet] Stripping antibodies To: cweston@valasciences.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <7690e6ad626d2.4eae0424@uwo.ca> Content-Type: text/plain; CHARSET=US-ASCII With quantum dots and a modern fluorescence microscope with "channels" it should be possible to label 2 or 3 antigens simultaneously, if you have all the right primaries and labelled secondaries. The company that sells you the quantum dot labelled secondaries or other amplification reagents should be in a position to tell you exactly what to do. Do they refund your hard-earned grant money if their expensive product fails to perform as advertized? Immunohistochemistry with non-fluorescent colours (available in brown, black, red and various blues) has advantages: (a) You do not need a fluorescence microscope. Modern fluorescence microscopes are very expensive; the old ones from the 1960s are no longer be good enough to provide publication-quality pictures. (b) Immunostained slides can be kept for many years in boxes at room temperature. (c) The colours do not fade in permanently mounted preparations. (c) You are not bound to use a commercially sold kit, which may not be optimized for your research. If you go by the book (= any book with references that you can follow up), you will do your multiple immunostains intelligently. Never follow a set of instructions without knowing or questioning the reason for ever step. John Kiernan Anatomy, UWO London, Canada. = = = On 30/10/11, Claire Weston wrote: > > > > Thank you everyone for your advice, I really appreciate it! I am doing a multiplex immunofluorescent assay (4 channels) and I am planning to strip the antibodies and reprobe with a second round of antibodies. The antibodies are labelled with quantum dots so I am not sure how that will influence things, but luckily I won't have the DAB problem several of you mentioned. I think I will try several of these techniques and evaluate which works the best. > > Thanks again for all your help, > > Claire > > --- jkiernan@uwo.ca wrote: > > From: John Kiernan > To: histonet@lists.utsouthwestern.edu, cweston@valasciences.com > Cc: Amos Brooks > Subject: Re: [Histonet] Stripping antibodies > Date: Sun, 30 Oct 2011 12:09:58 -0400 > > > > Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow. If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents. > > Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want? This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody. > > Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars). The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios, 1999, is very good. > > John Kiernan > Anatomy, UWO > London, Canada > = = = = > On 29/10/11, Amos Brooks wrote: > > > > > Hi, > > Stripping sites are usually found in the more seedy areas of large > > cities ... Oh wait you meant ... nevermind :-) If you developed the reaction > > with DAB, you may be in a difficult position. DAB is a really strong > > reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in > > 5% potassium premanganate for 5 min followed by 5% oxalic acid until it is > > clear (usually a couple of minutes). Unfortunately, I am not sure what > > effect this will have on the epitopes you are looking for, but this is > > usually what is used to clean precipitated DAB from instruments it should > > work on the tissue too. > > If you use another chromogen such as AEC it is much easier (to many > > people's dismay) to remove this end product. Using alkaline phosphatase will > > be easy to remove the chromogens as well. It would also be very easy to do > > this with fluorescent tags too. Just remove the coverslip and dip the slide > > in ethanol and it will remove the colored end product. Then just start over > > for the new antigen. > > > > Good luck, > > Amos > > > > On Fri, Oct 28, 2011 at 11:50 AM, > > wrote: > > > > > Message: 2 > > > Date: Thu, 27 Oct 2011 10:30:40 -0700 > > > From: "Claire Weston" > > > Subject: [Histonet] Stripping antibodies > > > To: > > > Message-ID: <002401cc94ce$2619ca60$724d5f20$@com> > > > Content-Type: text/plain; charset="us-ascii" > > > > > > Hi! > > > > > > > > > > > > Does anyone have experience in IHC stripping antibodies from a tissue > > > section and re-probing with different antibodies? What is the best way to > > > do that? If you could share your stripping protocol or experience I would > > > appreciate it - thanks! > > > > > > > > > > > > Claire > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > On 29/10/11, Amos Brooks wrote: > > > > > Hi, > > Stripping sites are usually found in the more seedy areas of large > > cities ... Oh wait you meant ... nevermind :-) If you developed the reaction > > with DAB, you may be in a difficult position. DAB is a really strong > > reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in > > 5% potassium premanganate for 5 min followed by 5% oxalic acid until it is > > clear (usually a couple of minutes). Unfortunately, I am not sure what > > effect this will have on the epitopes you are looking for, but this is > > usually what is used to clean precipitated DAB from instruments it should > > work on the tissue too. > > If you use another chromogen such as AEC it is much easier (to many > > people's dismay) to remove this end product. Using alkaline phosphatase will > > be easy to remove the chromogens as well. It would also be very easy to do > > this with fluorescent tags too. Just remove the coverslip and dip the slide > > in ethanol and it will remove the colored end product. Then just start over > > for the new antigen. > > > > Good luck, > > Amos > > > > On Fri, Oct 28, 2011 at 11:50 AM, > > wrote: > > > > > Message: 2 > > > Date: Thu, 27 Oct 2011 10:30:40 -0700 > > > From: "Claire Weston" > > > Subject: [Histonet] Stripping antibodies > > > To: > > > Message-ID: <002401cc94ce$2619ca60$724d5f20$@com> > > > Content-Type: text/plain; charset="us-ascii" > > > > > > Hi! > > > > > > > > > > > > Does anyone have experience in IHC stripping antibodies from a tissue > > > section and re-probing with different antibodies? What is the best way to > > > do that? If you could share your stripping protocol or experience I would > > > appreciate it - thanks! > > > > > > > > > > > > Claire > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > ------------------------------ Message: 4 Date: Mon, 31 Oct 2011 06:20:57 -0400 From: "Heath, Nancy L." Subject: RE: [Histonet] SALARY To: "Richard Cartun" , , , "Caroline Pratt" Message-ID: <130E8991F210424096EFC6F42EA33B240843564F@LSCOEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="us-ascii" "Histotechnologists are the most valuable employees in the laboratory today!" THANK YOU DR. CARTUN!! Nancy Heath, HT (ASCP) Neuropathology Technician Neuropathology Department Rhode Island Hospital President - Rhode Island Society for Histotechnology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 28, 2011 12:52 PM To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Caroline Pratt Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 31 Oct 2011 06:29:12 -0400 From: "Heath, Nancy L." Subject: RE: [Histonet] SALARY To: "John Shelley" , "Pratt, Caroline" , "Richard Cartun" , , Message-ID: <130E8991F210424096EFC6F42EA33B2408435651@LSCOEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Just got offered a lead tech position, salary of $85600.00 in a private lab opened by a group of docs. Didn't take it because the health insurance plan they offered was lousey. 75% health covered with a 250.00 deductable, dental covered at 60% and no coverage for family or husband....would of had to pay that out of pocket. I'm in New England. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Friday, October 28, 2011 2:25 PM To: Pratt, Caroline; Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY With all that said what are your going rates in this area that you are speaking. Kind Regards! ? John J Shelley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Friday, October 28, 2011 2:19 PM To: Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY If that is an option, I would agree, but non-profit teaching hospitals have compensation ranges and grids according to education and experience. We keep every employee with the same education and skill set at the same compensation for an equitable pay system. If we feel our employees are being under paid we will conduct a market analysis and the rates will be increased for all applicable employees if the market analysis justifies it. Car :) -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Friday, October 28, 2011 12:52 PM To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, Caroline Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------------------------------------------------ - This message was secured by ZixCorp(R). The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 31 Oct 2011 06:30:04 -0400 From: "Heath, Nancy L." Subject: RE: [Histonet] SALARY To: "Breeden, Sara" , "Podawiltz, Thomas" , "Richard Cartun" , , , "Caroline Pratt" Message-ID: <130E8991F210424096EFC6F42EA33B2408435652@LSCOEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="us-ascii" I agree :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, October 28, 2011 3:22 PM To: Podawiltz, Thomas; Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Caroline Pratt Subject: RE: [Histonet] SALARY I nominate Dr. Cartun as the Patron Saint for Histopathology. We should work on a statue and a Mission Statement for him... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 31 Oct 2011 06:33:01 -0600 From: "Breeden, Sara" Subject: [Histonet] Must be Monday... To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFB81@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ------------------------------ Message: 8 Date: Mon, 31 Oct 2011 08:40:02 -0400 From: "Blazek, Linda" Subject: [Histonet] RE: Must be Monday... To: "'Breeden, Sara'" , "Histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E39127401B441@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" That's a hoot Sally! I don't think that DST of old was even this past weekend. For the past 3 years my processor changed on the DST of old and the powers that be told me it was impossible because that wasn't programmed into my processor's data. This year I forgot about it and it didn't change on the old date. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 8:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 31 Oct 2011 08:01:31 -0500 From: "Hale, Meredith" Subject: [Histonet] Florida Medical Directorship To: Message-ID: <6F33D8418806044682A391273399860F0A5B2974@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" What are the true regulations' in Florida as far as time required for a Medical Director to be on site at the lab ? I am finding conflicting information and would like to know what is truly required. Thanks Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 ------------------------------ Message: 10 Date: Mon, 31 Oct 2011 09:56:10 -0400 From: Igor Deyneko Subject: [Histonet] Problems with Frozen tissues To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I'm looking for some advice on frozen tissues. This is the first time I'm doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut well onto special Gold Plus slides from Fisher. Then, when I was ready to stain the slides, i air dried them fro an hour and wanted to do H&E and Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on preventing this mischief? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ------------------------------ Message: 11 Date: Mon, 31 Oct 2011 14:02:04 +0000 From: "C.M. van der Loos" Subject: [Histonet] Stripping antibodies To: "histonet@lists.utsouthwestern.edu" Cc: "'amosbrooks@gmail.com'" Message-ID: Content-Type: text/plain; charset="us-ascii" Amos and John, I do not agree with John saying that "antibodies could be easily removed by acidity pH1-2". High affinity primaries will stay at the tissue section nicely bound to the epitope. Try for example SMA, clone 1A4 antibody in the usual dilution. After this incubation strip off the antibody by glycin-HCl buffer pH2 and then apply an anti-mouse detection system. You will stain SMA for sure. The only way to get antibodies off is 10 min boiling with citrate pH6.0 or so, as in HIER. I have described that in JOH in 2008, vol 31, pp. 119-127, being the basis of an AP sequential double staining. Chris van der Loos Academic Medical Center Dept. of Pathology M2-230 Amsterdam The Netherlands Date: Sun, 30 Oct 2011 12:09:58 -0400 From: John Kiernan Subject: Re: [Histonet] Stripping antibodies To: histonet@lists.utsouthwestern.edu, cweston@valasciences.com Cc: Amos Brooks Message-ID: <7640acec48487.4ead3e96@uwo.ca> Content-Type: text/plain; CHARSET=US-ASCII Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow. If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents. Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want? This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody. Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars). The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios, 1999, is very good. John Kiernan Anatomy, UWO London, Canada ------------------------------ Message: 12 Date: Mon, 31 Oct 2011 09:22:38 -0500 From: "Beckham, Sharon" Subject: [Histonet] RE: Must be Monday... To: "'Blazek, Linda'" , "'Breeden, Sara'" , "Histonet@lists.utsouthwestern.edu" Message-ID: <2C40E43D1F7A56408C4463FD245DDDF98AEE2C42@EXCHMB-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" Yes it was this past weekend back when. My alarm clock went to DST Sunday morning. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, October 31, 2011 7:40 AM To: 'Breeden, Sara'; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Must be Monday... That's a hoot Sally! I don't think that DST of old was even this past weekend. For the past 3 years my processor changed on the DST of old and the powers that be told me it was impossible because that wasn't programmed into my processor's data. This year I forgot about it and it didn't change on the old date. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 8:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 31 Oct 2011 09:25:58 -0500 From: "Mayer,Toysha N" Subject: [Histonet] RE: SALARY To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Add location to that. $28-32 is great for places that do not have an abundant supply of techs. Here in Houston, we have 2 schools and a very large medical center. Entry level techs are not hard to find. Graduates expect $23-28 minimum depending on facility. In other states, neighboring Louisiana and such, they expect $21-24. It just depends on the cost of living in the area. Entry supervisors earn $30+ an hour, but again, it is based on cost of living and work conditions. Reference labs pay more, but expect more. My first supervisor job paid $32, and I worked like crazy. It wasn't worth it. You get what you pay for though, and in this economic climate, the high salaries are not as plentiful as they once were. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Message: 6 Date: Fri, 28 Oct 2011 12:52:23 -0400 From: "Richard Cartun" Subject: RE: [Histonet] SALARY To: ,, "Caroline Pratt" Message-ID: <4EAAA586.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 95, Issue 34 **************************************** ------------------------------ Message: 14 Date: Mon, 31 Oct 2011 14:35:21 +0000 From: joelle weaver Subject: RE: [Histonet] SALARY To: , , , , , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I know the cost of living is greater on the east coast, but that sounds good to me. The insurance is not better, and as an HTL I make less than 1/2 of that! Things are determined by the market I realize, but all these postings are sure making me feel bad and also realize that I am not marketing myself well.... Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Mon, 31 Oct 2011 06:29:12 -0400 > From: NHeath@Lifespan.org > To: jshelley@sanfordburnham.org; Caroline.Pratt@uphs.upenn.edu; Rcartun@harthosp.org; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] SALARY > CC: > > Just got offered a lead tech position, salary of $85600.00 in a private lab opened by a group of docs. Didn't take it because the health insurance plan they offered was lousey. 75% health covered with a 250.00 deductable, dental covered at 60% and no coverage for family or husband....would of had to pay that out of pocket. > I'm in New England. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley > Sent: Friday, October 28, 2011 2:25 PM > To: Pratt, Caroline; Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] SALARY > > With all that said what are your going rates in this area that you are speaking. > > Kind Regards! > > John J Shelley > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline > Sent: Friday, October 28, 2011 2:19 PM > To: Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] SALARY > > If that is an option, I would agree, but non-profit teaching hospitals > have compensation ranges and grids according to education and > experience. We keep every employee with the same education and skill > set at the same compensation for an equitable pay system. If we feel > our employees are being under paid we will conduct a market analysis and > the rates will be increased for all applicable employees if the market > analysis justifies it. > > Car :) > > -----Original Message----- > From: Richard Cartun [mailto:Rcartun@harthosp.org] > Sent: Friday, October 28, 2011 12:52 PM > To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, > Caroline > Subject: RE: [Histonet] SALARY > > I think that's low. If you find a good candidate with years of > experience I would pay them whatever it takes to get them in the door. > It's like "Free Agency" in baseball; if you want a good player, you need > to put the "money on table". Histotechnologists are the most valuable > employees in the laboratory today! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> "Pratt, Caroline" 10/28/2011 9:21 AM > >>> > I would say $28 to $32 dollars an hour depending on experience and > education. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > kcastillo@frii.com > Sent: Friday, October 28, 2011 7:54 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] SALARY > > HI EVERYONE, > > WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID > THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM > LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this e-mail message is intended only for > the personal and confidential use of the recipient(s) named above. If > the reader of this message is not the intended recipient or an agent > responsible for delivering it to the intended recipient, you are hereby > notified that you have received this document in error and that any > review, dissemination, distribution, or copying of this message is > strictly prohibited. If you have received this communication in error, > please notify us immediately by e-mail, and delete the original message. > > > > > > ------------------------------------------------------------------------ > - > This message was secured by ZixCorp(R). > > > The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Mon, 31 Oct 2011 14:36:26 +0000 From: Helen Fedor Subject: [Histonet] RE: need help with TMA To: 'Patricia F Lott' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello, Do 30 minutes at 37 degrees on a glass slide. take from oven and apply gentle pressure to center of block and slide complex, cool to RT and repeat 1 or two times. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia F Lott Sent: Friday, October 28, 2011 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] need help with TMA We have recently started doing tissue microarrays. The problem we have is that some of the small cores drop out after the first few sections. I think my problem is in the step where we are supposed to melt the donor and recipient blocks together. We tried 5 minutes at 37 degrees, and then minutes at 60 degrees x3, with cooling to room temp in between. Any suggestions? Thanks, Patty Lott, UAB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Mon, 31 Oct 2011 14:40:13 +0000 From: joelle weaver Subject: RE: [Histonet] RE: SALARY To: , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Still sounds good to me, at my first supervisor's job ( the credentials I had at the time were HTL + Bachelor's), and I made less than $23 per hour and worked an average of 16 hours a day with no OT or comp time that was ever granted ( though promised). I guess I am just trying to say, count your blessings. Having a school near by really seems to impact the supply and demand and the general hiring environment. What I have noticed also is that when you go somewhere and the cost of living is less the tax burden is greater, so it has always seemed to come "out in the wash". Anyhow, all these salary revelations have really made me think, thanks for the postings. Joelle Weaver MAOM, BA, (HTL) ASCP > From: TNMayer@mdanderson.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 31 Oct 2011 09:25:58 -0500 > Subject: [Histonet] RE: SALARY > > Add location to that. $28-32 is great for places that do not have an abundant supply of techs. Here in Houston, we have 2 schools and a very large medical center. Entry level techs are not hard to find. Graduates expect $23-28 minimum depending on facility. > In other states, neighboring Louisiana and such, they expect $21-24. It just depends on the cost of living in the area. > Entry supervisors earn $30+ an hour, but again, it is based on cost of living and work conditions. Reference labs pay more, but expect more. > > My first supervisor job paid $32, and I worked like crazy. It wasn't worth it. > You get what you pay for though, and in this economic climate, the high salaries are not as plentiful as they once were. > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > Message: 6 > Date: Fri, 28 Oct 2011 12:52:23 -0400 > From: "Richard Cartun" > Subject: RE: [Histonet] SALARY > To: ,, > "Caroline Pratt" > Message-ID: <4EAAA586.7400.0077.1@harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> > I would say $28 to $32 dollars an hour depending on experience and education. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com > Sent: Friday, October 28, 2011 7:54 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] SALARY > > HI EVERYONE, > > WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID > THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM > LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 95, Issue 34 > **************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Mon, 31 Oct 2011 10:33:12 -0400 (EDT) From: "Nicole Tatum" Subject: [Histonet] background checks required by AHCA To: histonet@lists.utsouthwestern.edu Message-ID: <1533.208.62.167.196.1320071592.squirrel@webmail.realpages.com> Content-Type: text/plain;charset=iso-8859-1 I am currently filing out my ahca renewal application. There are many changes and the form and I do not find it user friendly. Anyways here my question: Medical directors and chief finicial officers or any person who stands to gain profit must undergo a level 2 background screeening. On the new app is states all empolyees and health care providers. I called acha licensing division and they said only directors and so forth. But, on ahca website statue 408(something, ill have to get exact number) when into effect in 2010 that says all employees and newly hired employees must underground background screening. So does all the arnp, pa, ht, and ma's need to be screened because we have a lab? Nicole Tatum, HT ASCP http://ahca.myflorida.com/mchq/long_term_care/Background_Screening/BGS_WhoRequiredToBeScreened.pdf taken directly from ahca site: Employees and Contractors employed before August 1, 2010 Every employee/contractor must attest to meeting the requirements of this chapter and agreeing to inform the employer immediately if arrested for any of the disqualifying offenses while employed by the employer. [Section 435.05(2)]. This attestation must be maintained in the employee?s personnel file. You may use the Affidavit of Compliance with Background Screening to satisfy the attestation requirement. If an employer becomes aware that an employee/contractor has been arrested for a disqualifying offense, the employer must remove the employee/contractor from contact with any vulnerable person that places the employee/contractor in a role that requires background screening until the arrest is resolved in a way that the employer determines that the employee/contractor is still eligible for employment/contracting under this chapter. [Section 435.06(2)(b)] Rescreening ------------------------------ Message: 18 Date: Mon, 31 Oct 2011 10:56:17 -0500 From: "Orr, Rebecca" Subject: [Histonet] Dako Pr on Ventana? To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Friends, If anyone is using Dako PR concentrate clone 1294 on a Ventana, could you please contact me separately? I'd like some advice. Thank you Becky Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 -----Original Message----- Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 95, Issue 37 **************************************** From Lynn.Burton <@t> Illinois.gov Mon Oct 31 15:55:16 2011 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Mon Oct 31 15:56:03 2011 Subject: [Histonet] RE: Automated coverslipper with Ventana labels In-Reply-To: <9F3CFEE76E51B64991C7485270890B4009ED52D1@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B4009ED52D1@EX5.lj.gnf.org> Message-ID: <4A6E2CACA1E017408EBA1B9911952CC00645680B85@IL084EXMBX214.illinois.gov> We have a 15 year old Sakura film coverslipper that has performed famously with Venatana labels. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Theresa (Teri) Johnson [TJohnson@gnf.org] Sent: Monday, October 31, 2011 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated coverslipper with Ventana labels Hi all, I wanted to ask if any of you out there are currently using an automated coverslipper and do not have issues with the forks picking up the slides if they have Ventana labels on them. I have used a Leica and had only very occasionally a dropped slide. Is this your experience as well? Are there other units that would work well for this? Feel free to contact me off list if you need to. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet