[Histonet] MAP-2 Troubleshooting

[Erin Sarricks]

Hi Histonet-



I am posting this question for a colleague of mine who is having some
problems performing a MAP-2 stain.





Problem:  Blotchy or "wormy" areas of light or absent staining of MAP-2
(Thermo Scientific MS-250-R7) in brain sections by immunohistochemistry.
Microwave antigen retrieval was performed using 10 mM citric acid at pH 6.
The stain worked very well historically. Charged slides were stained by
immersion or individually using PAP barrier. The areas of stain loss are
repetitive on neighboring slides, which suggests antigen retrieval as the
problem step. Several attempts at modifying the retrieval process have not
been unsuccessful.




Has anyone else been running into these issues?  Any advice or tips would
be greatly appreciated!  Thank you.



Erin Sarricks, HT (ASCP)

Histology Team Leader

USAMRICD Comparative Pathology Branch

Office: Bldg E-3081 Room 178

E-mail: erin.p.sarricks <@t> us.army.mil

Phone: 410-436-1967