From epchatfield <@t> ihis.org Tue Nov 1 06:31:10 2011 From: epchatfield <@t> ihis.org (Elizabeth Chatfield) Date: Tue Nov 1 06:31:33 2011 Subject: [Histonet] In Newfoundland Canada the scale is $32-42 / hour. Message-ID: <4EAFAE4E020000BC0001184C@smtp1.gov.pe.ca> In Newfoundland Canada the scale is $32-42 / hour. Elizabeth ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From thiggins <@t> cddmedical.com Tue Nov 1 11:34:50 2011 From: thiggins <@t> cddmedical.com (Tim Higgins) Date: Tue Nov 1 11:34:55 2011 Subject: [Histonet] Spirochete Control Block Message-ID: <005501cc98b4$2d495e60$e001a8c0@cdd.loc> Hello Histonetters, Anyone have a spirochete control block(s) they would like to trade? Thanks, Tim From Ashley.Troutman <@t> Vanderbilt.Edu Tue Nov 1 11:52:01 2011 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Tue Nov 1 11:52:13 2011 Subject: [Histonet] Automated coverslipper with Ventana labels Message-ID: <7B310892042DA74CB3590053F424CFE61448AA5546@ITS-HCWNEM06.ds.Vanderbilt.edu> In my experience with the Leica coverslipper, I have to hand coverslip all Ventana slides. It throws more than it coverslips. I have tried running them down quickly and avoiding Xylene on the labels, but that works only marginally better. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 1 Date: Mon, 31 Oct 2011 17:04:43 +0000 From: "Theresa (Teri) Johnson" Subject: [Histonet] Automated coverslipper with Ventana labels To: "histonet@lists.utsouthwestern.edu" Message-ID: <9F3CFEE76E51B64991C7485270890B4009ED52D1@EX5.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" Hi all, I wanted to ask if any of you out there are currently using an automated coverslipper and do not have issues with the forks picking up the slides if they have Ventana labels on them. I have used a Leica and had only very occasionally a dropped slide. Is this your experience as well? Are there other units that would work well for this? Feel free to contact me off list if you need to. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From pruegg <@t> ihctech.net Tue Nov 1 12:07:47 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Nov 1 12:07:52 2011 Subject: [Histonet] mast cells for arteries Message-ID: <4937809CEF224CCE99B75C4F01EC4D5E@Patsyoffice> Does anyone have a go to stain for Mast Cells besides Tol Blue? We are trying to demonstrate the presence of mast cells in human arteries ffpe. Also, what would be a good positive control to use for mast cell staining, we used NH Skin and got maybe a few mast cells but I would like to use something more abundant with mast cells. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From turkekul <@t> gmail.com Tue Nov 1 12:09:34 2011 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Tue Nov 1 12:09:41 2011 Subject: [Histonet] Leica coverslipper and Ventana slides Message-ID: I use histoclear in the last step of dehydration and have no problem with the arm picking up the slides. If I leave the slides for more than one hour in the last histoclear and if the histoclear covers the labels, then the glue of the label dissolves and interferes with the arm picking up the slides. Also If the labels are not places carefully on the slides and stick out from the slides then the arm has difficulty picking up the slides. Make sure your labels are placed carefully and use histoclear. If you have to use xylene make sure the xylene level is not very high so that It can affect the labels. 3 xylene changes 1 minute each is enough. Mesru From tajibade <@t> echd.org Tue Nov 1 12:15:48 2011 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Tue Nov 1 12:17:01 2011 Subject: [Histonet] HISTOTECHS ARE NEEDED IN TEXAS In-Reply-To: References: Message-ID: I have an opening position for histotech.This is a full time and permanent position; if you are interested please call me or e-mail your resume. Thanks Tunde Ajibade BS, HTL(ASCP)QIHC Histology Supervisor Medical Center Hospital Odessa,TX Email:tajibade@echd.org Tel:432-640-2348 Fax:432-640-2303 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Tuesday, November 01, 2011 12:02 PM To: Tunde Ajibade Subject: Histonet@lists.utsouthwestern.edu mailing list reminder You, or someone posing as you, has requested a password reminder for your membership on the mailing list histonet@lists.utsouthwestern.edu. You will need this password in order to change your membership options (e.g. do you want regular delivery or digest delivery), and having this password makes it easier for you to unsubscribe from the mailing list. You are subscribed with the address: tajibade@echd.org Your Histonet password is: ooooo To make changes to your membership options, log in and visit your options web page: http://lists.utsouthwestern.edu/mailman/options/histonet/tajibade%40echd.org You can also make such changes via email by sending a message to: histonet-request@lists.utsouthwestern.edu with the text "help" in the subject or body. The automatic reply will contain more detailed instructions. Questions or comments? Please send them to the Histonet mailing list administrator at histonet-owner@lists.utsouthwestern.edu. CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. 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From llewllew <@t> shaw.ca Tue Nov 1 12:25:02 2011 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Nov 1 12:25:09 2011 Subject: [Histonet] mast cells for arteries In-Reply-To: <4937809CEF224CCE99B75C4F01EC4D5E@Patsyoffice> References: <4937809CEF224CCE99B75C4F01EC4D5E@Patsyoffice> Message-ID: <4EB02B6E.9000105@shaw.ca> Go to http://stainsfile.info/StainsFile/stain/cell/aldtolblue.htm for a reliable method. It still uses toluidine blue, but the staining is not based on metachromasia and mast cell granules are dark blue. Most toluidine blue samples will work, but occasionally one doesn't. Fisher brand is OK as are many others. I used to use a section from an acute appendicitis case as the control. Bryan Llewellyn Patsy Ruegg wrote: > Does anyone have a go to stain for Mast Cells besides Tol Blue? We are > trying to demonstrate the presence of mast cells in human arteries ffpe. > Also, what would be a good positive control to use for mast cell staining, > we used NH Skin and got maybe a few mast cells but I would like to use > something more abundant with mast cells. > > > > Best regards, > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that is > privileged& confidential within the meaning of applicable law. Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. If > you are NOT the intended recipient please contact the sender and dispose of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From SEsparza <@t> seton.org Tue Nov 1 12:29:57 2011 From: SEsparza <@t> seton.org (Esparza, Sandra) Date: Tue Nov 1 12:30:02 2011 Subject: [Histonet] Automated coverslipper with Ventana labels In-Reply-To: <7B310892042DA74CB3590053F424CFE61448AA5546@ITS-HCWNEM06.ds.Vanderbilt.edu> References: <7B310892042DA74CB3590053F424CFE61448AA5546@ITS-HCWNEM06.ds.Vanderbilt.edu> Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB041DFE63@AUSEX2VS1.seton.org> I use to have a problem with the labels from the Ventana slides on the Leica Coverslipper. I now use the Trubond 200 slides which are a little wider (76x26mm instead of the standard 76x25mm) this keeps the labels from hanging off the sides and it seems to work on the Leica Coverslipper. Sandra Sandra Esparza HT(ASCP)QIHC Lead Technologist Dell Children's Medical Center of Central Texas 512-324-0000 x87061 sesparza@seton.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troutman, Kenneth A Sent: Tuesday, November 01, 2011 11:52 AM To: Histonet@lists.utsouthwestern.edu Cc: 'TJohnson@gnf.org' Subject: RE: [Histonet] Automated coverslipper with Ventana labels In my experience with the Leica coverslipper, I have to hand coverslip all Ventana slides. It throws more than it coverslips. I have tried running them down quickly and avoiding Xylene on the labels, but that works only marginally better. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 1 Date: Mon, 31 Oct 2011 17:04:43 +0000 From: "Theresa (Teri) Johnson" Subject: [Histonet] Automated coverslipper with Ventana labels To: "histonet@lists.utsouthwestern.edu" Message-ID: <9F3CFEE76E51B64991C7485270890B4009ED52D1@EX5.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" Hi all, I wanted to ask if any of you out there are currently using an automated coverslipper and do not have issues with the forks picking up the slides if they have Ventana labels on them. I have used a Leica and had only very occasionally a dropped slide. Is this your experience as well? Are there other units that would work well for this? Feel free to contact me off list if you need to. Thanks! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kevin_Kurtz <@t> ssmhc.com Tue Nov 1 12:49:16 2011 From: Kevin_Kurtz <@t> ssmhc.com (Kurtz, Kevin) Date: Tue Nov 1 12:51:40 2011 Subject: [Histonet] Heat Extractor for Frozen Sections Message-ID: During residency, we prepared frozen sections by placing OCT and tissue on a precooled specimen disc and then putting a heat extractor on top, which both rapidly froze the tissue and created a flat surface. This seemed to work best when the heat extractor was slightly larger in diameter than the specimen disc, but I've been having trouble finding heat extractors in different sizes. Our specimen discs are 25 and 30 mm in diameter. Does anyone know where I could order heat extractors that are larger in diameter? Thank you! Kevin Kurtz, M.D. St. Mary's Hospital Madison, Wisconsin Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From foreightl <@t> gmail.com Tue Nov 1 13:23:56 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Nov 1 13:24:00 2011 Subject: [Histonet] mast cells for arteries In-Reply-To: <4937809CEF224CCE99B75C4F01EC4D5E@Patsyoffice> References: <4937809CEF224CCE99B75C4F01EC4D5E@Patsyoffice> Message-ID: A good one that I used to use was the Naphthol AS-D Chloroacetate-esterase which stains the non specific esterase activity in mast cells. It is a pain to run, but it is one of the most beautiful. A bright red mast cell against a pale background. We got it from Vacca, p 530-533. On Tue, Nov 1, 2011 at 10:07 AM, Patsy Ruegg wrote: > Does anyone have a go to stain for Mast Cells besides Tol Blue? We are > trying to demonstrate the presence of mast cells in human arteries ffpe. > Also, what would be a good positive control to use for mast cell staining, > we used NH Skin and got maybe a few mast cells but I would like to use > something more abundant with mast cells. > > > > Best regards, > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that > is > privileged & confidential within the meaning of applicable law. Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. If > you are NOT the intended recipient please contact the sender and dispose of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From nicole <@t> dlcjax.com Tue Nov 1 13:47:08 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Tue Nov 1 13:59:48 2011 Subject: [Histonet] Problems with Frozen tissues In-Reply-To: <1320076911.80808.YahooMailNeo@web112303.mail.gq1.yahoo.com> References: <1320076911.80808.YahooMailNeo@web112303.mail.gq1.yahoo.com> Message-ID: <3451.208.62.167.196.1320173228.squirrel@webmail.realpages.com> Igor, I perform Mohs which is a frozen section procedure. There are a couple of things that could help you. You do not have to leave the slides out to airdry for an H&E only do this is a specfic procedure requires it. Do not places slides strait into water. As Kim, stated some people use a fixative prior to staining. I place slides into 95% alcohol and then they are rinsed. The next thing to consider is how thick you are cutting the tissue. It can be difficult to keep thick, fatty, or tissue with alot of cartiladge on the slide. So cut as thin as possible. Ok next is the oct or mounting media ur using. In between each section you should wipe the oct. OCT is water solauble. If the tissue is placed over OCT on the slide it will definitly wash off. So if you are trying to put multiple sctions on a slide, Place enough room between them so the tissue is directly placed on the clean surface of the charged slide, or wipe excess oct in between sections. Last note. If you have automatic stainer and it has an aggitate function.Turn it off. Stain the slides as gently as possible. Hope this helps, Nicole Tatum HT ASCP Ive never just air dried my frozen sections. always put them in a fixative > such as pen fix, a alcohol and or formalin mixture, something( depends on > what your going to look for, test etc). That with using charged slides and > never had too many problems with this. > ? > Kim > > > ________________________________ > From: Igor Deyneko > To: Histonet@lists.utsouthwestern.edu > Sent: Monday, October 31, 2011 9:56 AM > Subject: [Histonet] Problems with Frozen tissues > > I'm looking for some advice on frozen tissues. This is the first time I'm > doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut > well onto special Gold Plus slides from Fisher. Then, when I was ready to > stain the slides, i air dried them fro an hour and wanted to do H&E and > Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on > preventing this mischief? > Thank you in advance. > Igor Deyneko > Infinity Pharmaceuticals > Cambridge, MA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Gina.Rodriguez <@t> leica-microsystems.com Tue Nov 1 16:02:37 2011 From: Gina.Rodriguez <@t> leica-microsystems.com (Gina.Rodriguez@leica-microsystems.com) Date: Tue Nov 1 16:02:45 2011 Subject: [Histonet] AUTO: Gina Rodriguez is out of the office. (returning 11/07/2011) Message-ID: I am out of the office until 11/07/2011. I will respond to your message when I return. If you need immediate assistance please contact 800-248-0123 or Tech.support@leica-microsystems.com Note: This is an automated response to your message "Histonet Digest, Vol 96, Issue 1" sent on 11/1/2011 11:36:42 AM. This is the only notification you will receive while this person is away. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From daniela.bodemer <@t> mcri.edu.au Tue Nov 1 17:16:09 2011 From: daniela.bodemer <@t> mcri.edu.au (Daniela Bodemer) Date: Tue Nov 1 17:16:17 2011 Subject: [Histonet] Problems with Frozen tissues In-Reply-To: <1320076911.80808.YahooMailNeo@web112303.mail.gq1.yahoo.com> References: <1320076911.80808.YahooMailNeo@web112303.mail.gq1.yahoo.com> Message-ID: <9DF797D618351549B984596F01A1FE1D01FCC75D@murmx.mcri.edu.au> Our tissues are frozen in OCT. I use Super Frost Plus slides from Thermo Scientific and store the sections in the fridge until I need them. After leaving them out on the bench for an hour I start my H&E with 1 min running water and so on. I rarely lose sections with these slides. Daniela -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, 1 November 2011 3:02 AM To: Igor Deyneko; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Problems with Frozen tissues Ive never just air dried my frozen sections. always put them in a fixative such as pen fix, a alcohol and or formalin mixture, something( depends on what your going to look for, test etc). That with using charged slides and never had too many problems with this. ? Kim ________________________________ From: Igor Deyneko To: Histonet@lists.utsouthwestern.edu Sent: Monday, October 31, 2011 9:56 AM Subject: [Histonet] Problems with Frozen tissues I'm looking for some advice on frozen tissues. This is the first time I'm doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut well onto special Gold Plus slides from Fisher. Then, when I was ready to stain the slides, i air dried them fro an hour and wanted to do H&E and Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on preventing this mischief? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email If you have any question, please contact MCRI IT Helpdesk for further assistance. ______________________________________________________________________ ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From jhabecke <@t> fhcrc.org Tue Nov 1 17:49:29 2011 From: jhabecke <@t> fhcrc.org (Randolph-Habecker, Julie) Date: Tue Nov 1 17:49:37 2011 Subject: [Histonet] Luciferase IHC Message-ID: <040346FA7309BD439C327F97D4C4D69B0D78BF28@ISIS.fhcrc.org> Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabecke@fhcrc.org From one_angel_secret <@t> yahoo.com Tue Nov 1 18:53:57 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Nov 1 18:54:01 2011 Subject: [Histonet] Problems with Frozen tissues In-Reply-To: <9DF797D618351549B984596F01A1FE1D01FCC75D@murmx.mcri.edu.au> References: <1320076911.80808.YahooMailNeo@web112303.mail.gq1.yahoo.com> <9DF797D618351549B984596F01A1FE1D01FCC75D@murmx.mcri.edu.au> Message-ID: <1320191637.61068.YahooMailNeo@web112304.mail.gq1.yahoo.com> may I ask why you are not fixing them? Even the stain the 1st gentlman was doing(Beta Gal)?recomended to have a formalin fixation apllied first. ? There are many ways to get to the same show as we all know, But in my? experiance air drying frozen sections will dry out and cells can autolyse as the process of autolysation has not been stopped, also vacouls in the tissue can develope?from the process of dehydration without the use of fixation< sorry for spelling, on lap top on dark porch. ? Are you trying to minumize usage of alcohols or other fixatives? ? PLease explain to me if you would because I have cut just about all kinds of tissues for all kinds of procedures and never have I heard to keep a fresh section out for an hour before staining and without a fixative. I love ot learn new things ) ? Thanks ? Kim ________________________________ From: Daniela Bodemer To: Kim Donadio ; Igor Deyneko ; Histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:16 PM Subject: RE: [Histonet] Problems with Frozen tissues Our tissues are frozen in OCT. I use Super Frost Plus slides from Thermo Scientific and store the sections in the fridge until I need them. After leaving them out on the bench for an hour I start my H&E with 1 min running water and so on. I rarely lose sections with these slides. Daniela -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, 1 November 2011 3:02 AM To: Igor Deyneko; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Problems with Frozen tissues Ive never just air dried my frozen sections. always put them in a fixative such as pen fix, a alcohol and or formalin mixture, something( depends on what your going to look for, test etc). That with using charged slides and never had too many problems with this. ? Kim ________________________________ From: Igor Deyneko To: Histonet@lists.utsouthwestern.edu Sent: Monday, October 31, 2011 9:56 AM Subject: [Histonet] Problems with Frozen tissues I'm looking for some advice on frozen tissues. This is the first time I'm doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut well onto special Gold Plus slides from Fisher. Then, when I was ready to stain the slides, i air dried them fro an hour and wanted to do H&E and Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on preventing this mischief? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email If you have any question, please contact MCRI IT Helpdesk for further assistance. ______________________________________________________________________ ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From one_angel_secret <@t> yahoo.com Tue Nov 1 19:00:50 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Nov 1 19:00:53 2011 Subject: [Histonet] Luciferase IHC In-Reply-To: <040346FA7309BD439C327F97D4C4D69B0D78BF28@ISIS.fhcrc.org> References: <040346FA7309BD439C327F97D4C4D69B0D78BF28@ISIS.fhcrc.org> Message-ID: <1320192050.55176.YahooMailNeo@web112304.mail.gq1.yahoo.com> You have to soak it in holy water, then all H311 will break out,,,, ? Sorry, I couldnt resist. ? ? ________________________________ From: "Randolph-Habecker, Julie" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabecke@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From daniela.bodemer <@t> mcri.edu.au Tue Nov 1 21:46:30 2011 From: daniela.bodemer <@t> mcri.edu.au (Daniela Bodemer) Date: Tue Nov 1 21:46:38 2011 Subject: [Histonet] DAB Immuno and alcian blue counter staining In-Reply-To: <9DF797D618351549B984596F01A1FE1D01FCC75D@murmx.mcri.edu.au> References: <1320076911.80808.YahooMailNeo@web112303.mail.gq1.yahoo.com> <9DF797D618351549B984596F01A1FE1D01FCC75D@murmx.mcri.edu.au> Message-ID: <9DF797D618351549B984596F01A1FE1D01FCC8D2@murmx.mcri.edu.au> Hi all, Is it possible to do an immuno with DAB detection and counterstain it with alcian blue at the end to detect Goblets cells? I've tried it and the DAB disappears under the staining. Thanks, Daniela ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Carmen.M.Garcia <@t> uv.es Wed Nov 2 03:41:50 2011 From: Carmen.M.Garcia <@t> uv.es (Carmen Maria Garcia Pascual) Date: Wed Nov 2 03:42:02 2011 Subject: [Histonet] Problems with Frozen tissues In-Reply-To: <9DF797D618351549B984596F01A1FE1D01FCC75D@murmx.mcri.edu.au> References: <9DF797D618351549B984596F01A1FE1D01FCC75D@murmx.mcri.edu.au> Message-ID: <2483892948carmaga6@uv.es> Good morning! I work with ovarie and mice uterus embebbed in oct. I get 8um frozen sections and put them un super frost slides, the I leave the slides in the fridge until use them. When I am going to perform the immunohistochemistry I leave the slides 1 minute at rt to atemperate the tissue, then I place the slides 10 minutes in acetone (stored at - 20?C), whit this you fix the tissue and permeabilize it. with this protocol I never loss any sections. If you have any more question just write me. good luck, Carmen ******************************************************* Carmen Mar?a Garc?a Pascual FIVI/INCLIVA/Facultad de Medicina Universidad de Valencia Departamento de P.O.G (Laboratorios) 96.386.40.48 Avd. Blasco Iba?ez 17 46010, Valencia ******************************************************* From lanigac <@t> ccf.org Wed Nov 2 06:19:21 2011 From: lanigac <@t> ccf.org (Lanigan, Christopher) Date: Wed Nov 2 06:19:41 2011 Subject: [Histonet] EBER ISH Message-ID: <9DDD0F026DC71B41B79D8E439D7951A3097D8DF5@cchsclexmb69.cc.ad.cchs.net> Hi Sheila, Sorry for the delay. Yes, I am familiar with the EBER ISH probes on the Ventana Benchmark Ultra. Yes, the software that you will need to have installed is called "U - ISH OPEN PROBES - CHROMOGENIC v3". This protocol will use the BLUE DETECTION and with a RED STAIN II counterstain. Before I get to the protocol, I'll fill you in on the probes. They do NOT arrive in a dispenser. They come in pre-diluted vials, and you will need to fill a "user-fillable dispense". One more thing, you will need to run a control. This control will confirm the presence of non-degradated RNA. The control I used was U6, and the protocol is identical to EBER except for the selected ISH probe. By the way, the selected ISH probe will likely be "ISH PROBE 1" or "ISH PROBE 2" because the user-fillable dispenser will not have the proper commercial bar code. Finally, the following is a successful protocol summary (HybReady Soln is optional for high background): 1 Deparaffinization [Selected] 2 Warmup Slide to [69 Deg C], and Incubate for 16 Minutes ( Deparaffinization ) 3 Pretreatment [Selected] 4 CC2 [Selected] 5 CC2 Cycle 1 [Selected] 6 CC2 Cycle 2 [Selected] 7 Enzyme [Selected] 8 Apply One Drop of [ISH-PROTEASE 3] ( Enzyme ), and Incubate for [12 Minutes] 9 Probe [Selected] 10 Probe Auto Dispense [Selected] 11 Apply One Drop of [ISH Probe 5] ( ISH Probe ), No Coverslip and Incubate for 4 Minutes 12 Denature [Selected] 13 Warmup Slide to [75 Deg C], and Incubate for [4 Minutes] ( Denaturation ) 14 Hybridization [Selected] 15 Warmup Slide to [50 Deg C], and Incubate for 4 Minutes ( Hybridization ) 16 Incubate for [1 Hour] ( Hybridization ) 17 Stringency Washes [Selected] 18 Stringency Wash #1 [Selected] 19 Warmup Slide to [60 Deg C], and Incubate for [8 Minutes] ( Stringency Wash #1 ) 20 Stringency Wash #2 [Selected] 21 Incubate for [8 Minutes] ( Stringency Wash #2 ) 22 Stringency Wash #3 [Selected] 23 Incubate for [8 Minutes] ( Stringency Wash #3 ) 24 Detection Kit [Selected] 25 Blue Detection [Selected] 26 Incubate for [32 Minutes] ( Substrate ) 27 Counterstain [Selected] 28 Apply One Drop of [Red Stain II] ( Counterstain ), Apply Coverslip, and Incubate for [4 Minutes] Let me know if I forgot anything. Chris Lanigan Histonetters, Is anyone out there using the EBER ISH probes from Ventana on the Ultra Platform? I have a few questions. Did you need to get new software installed? Was there any training involved? And do you have successful protocols that you are willing to share? I have asked the questions before, but at the time there was nobody actually using it. I know that there were inquiries about the protocols because of the ASR status and the fact that Ventana cannot give you any advice. Any and all information would be greatly appreciated since we are wanting to start it up at our facility. Thanks, Sheila KDL Pathology Knoxville, TN Christopher Lanigan Research Technologist Molecular Pathology Cleveland Clinic Foundation 9500 Euclid Avenue L3-127 Cleveland, OH 44195 (216) 445-1443 =================================== Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S.News & World Report (2010). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From billodonnell <@t> catholichealth.net Wed Nov 2 08:19:05 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Nov 2 08:19:24 2011 Subject: [Histonet] Luciferase IHC In-Reply-To: <1320192050.55176.YahooMailNeo@web112304.mail.gq1.yahoo.com> References: <040346FA7309BD439C327F97D4C4D69B0D78BF28@ISIS.fhcrc.org> <1320192050.55176.YahooMailNeo@web112304.mail.gq1.yahoo.com> Message-ID: <4940DF6D1C5FDF48931B6966AAEF93952AC101@chimsx08.CHI.catholichealth.net> Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out,,,, ? Sorry, I couldnt resist. ? ? ________________________________ From: "Randolph-Habecker, Julie" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabecke@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Wed Nov 2 08:23:13 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Nov 2 08:23:23 2011 Subject: [Histonet] Luciferase IHC In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93952AC101@chimsx08.CHI.catholichealth.net> References: <040346FA7309BD439C327F97D4C4D69B0D78BF28@ISIS.fhcrc.org><1320192050.55176.YahooMailNeo@web112304.mail.gq1.yahoo.com> <4940DF6D1C5FDF48931B6966AAEF93952AC101@chimsx08.CHI.catholichealth.net> Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFB9B@nmdamailsvr.nmda.ad.nmsu.edu> Perfect!! And I was afraid Halloween was going to sneak by unnoticed! Which vendor sells holy water, 'cause I'm gonna need some... From trathborne <@t> somerset-healthcare.com Wed Nov 2 08:26:39 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Nov 2 08:26:23 2011 Subject: [Histonet] Luciferase IHC In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DFB9B@nmdamailsvr.nmda.ad.nmsu.edu> References: <040346FA7309BD439C327F97D4C4D69B0D78BF28@ISIS.fhcrc.org><1320192050.55176.YahooMailNeo@web112304.mail.gq1.yahoo.com> <4940DF6D1C5FDF48931B6966AAEF93952AC101@chimsx08.CHI.catholichealth.net> <4D14F0FC9316DD41972D5F03C070908B051DFB9B@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F5FF93@SMCMAIL01.somerset-healthcare.com> Haven't seen it in their catalog, but you could try Eeeeerie Scientific ; ) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, November 02, 2011 9:23 AM To: O'Donnell, Bill; Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Perfect!! And I was afraid Halloween was going to sneak by unnoticed! Which vendor sells holy water, 'cause I'm gonna need some... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From sbreeden <@t> nmda.nmsu.edu Wed Nov 2 08:26:50 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Nov 2 08:26:53 2011 Subject: [Histonet] Luciferase IHC In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB7570711F5FF93@SMCMAIL01.somerset-healthcare.com> References: <040346FA7309BD439C327F97D4C4D69B0D78BF28@ISIS.fhcrc.org><1320192050.55176.YahooMailNeo@web112304.mail.gq1.yahoo.com><4940DF6D1C5FDF48931B6966AAEF93952AC101@chimsx08.CHI.catholichealth.net> <4D14F0FC9316DD41972D5F03C070908B051DFB9B@nmdamailsvr.nmda.ad.nmsu.edu> <3AD061FE740D464FAC7BF6B5CFB7570711F5FF93@SMCMAIL01.somerset-healthcare.com> Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFB9C@nmdamailsvr.nmda.ad.nmsu.edu> OOOH!!! Why didn't I think of that! Good goin'! From SLB <@t> stowers.org Wed Nov 2 08:46:28 2011 From: SLB <@t> stowers.org (Beckham, Sharon) Date: Wed Nov 2 08:46:35 2011 Subject: [Histonet] Luciferase IHC In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF93952AC101@chimsx08.CHI.catholichealth.net> References: <040346FA7309BD439C327F97D4C4D69B0D78BF28@ISIS.fhcrc.org> <1320192050.55176.YahooMailNeo@web112304.mail.gq1.yahoo.com> <4940DF6D1C5FDF48931B6966AAEF93952AC101@chimsx08.CHI.catholichealth.net> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF98AEE2C53@EXCHMB-02.stowers-institute.org> You know sometimes I wish these emails had a "LIKE" button on them. I found this quite funny and wanted to "LIKE" it. Too much facebook for me! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 02, 2011 8:19 AM To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out,,,, ? Sorry, I couldnt resist. ? ? ________________________________ From: "Randolph-Habecker, Julie" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabecke@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Wed Nov 2 08:58:38 2011 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Wed Nov 2 08:58:52 2011 Subject: [Histonet] RE: mast cells for arteries Message-ID: Patsy, Just consider anti-tryptase antibody that is directly labeled with Alk. phos. (Millipore MAB1222A). Tris-EDTA pH9.0 pretreatment / 1:5000 overnight 4C / Vector Red. A tonsil will do as positive control. Good luck! Cheers, Chris Chris van der Loos Academic Medical Center Dept. of Pathology Amsterdam The Netherlands Date: Tue, 1 Nov 2011 11:07:47 -0600 From: "Patsy Ruegg" Subject: [Histonet] mast cells for arteries To: Message-ID: <4937809CEF224CCE99B75C4F01EC4D5E@Patsyoffice> Content-Type: text/plain; charset="us-ascii" Does anyone have a go to stain for Mast Cells besides Tol Blue? We are trying to demonstrate the presence of mast cells in human arteries ffpe. Also, what would be a good positive control to use for mast cell staining, we used NH Skin and got maybe a few mast cells but I would like to use something more abundant with mast cells. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 From sbreeden <@t> nmda.nmsu.edu Wed Nov 2 09:03:59 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Nov 2 09:04:05 2011 Subject: [Histonet] Luciferase IHC In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF98AEE2C53@EXCHMB-02.stowers-institute.org> References: <040346FA7309BD439C327F97D4C4D69B0D78BF28@ISIS.fhcrc.org><1320192050.55176.YahooMailNeo@web112304.mail.gq1.yahoo.com><4940DF6D1C5FDF48931B6966AAEF93952AC101@chimsx08.CHI.catholichealth.net> <2C40E43D1F7A56408C4463FD245DDDF98AEE2C53@EXCHMB-02.stowers-institute.org> Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFB9D@nmdamailsvr.nmda.ad.nmsu.edu> At times like these, I wonder where Joe Nocito is... From lblazek <@t> digestivespecialists.com Wed Nov 2 09:05:33 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Nov 2 09:05:42 2011 Subject: [Histonet] Luciferase IHC In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DFB9D@nmdamailsvr.nmda.ad.nmsu.edu> References: <040346FA7309BD439C327F97D4C4D69B0D78BF28@ISIS.fhcrc.org><1320192050.55176.YahooMailNeo@web112304.mail.gq1.yahoo.com><4940DF6D1C5FDF48931B6966AAEF93952AC101@chimsx08.CHI.catholichealth.net> <2C40E43D1F7A56408C4463FD245DDDF98AEE2C53@EXCHMB-02.stowers-institute.org> <4D14F0FC9316DD41972D5F03C070908B051DFB9D@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E39127401B469@IBMB7Exchange.digestivespecialists.com> Yeah! We've really missed JTT! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, November 02, 2011 10:04 AM To: Beckham, Sharon; O'Donnell, Bill; Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC At times like these, I wonder where Joe Nocito is... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Wed Nov 2 09:08:55 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Nov 2 09:08:59 2011 Subject: [Histonet] Where are you, JTT? Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFB9E@nmdamailsvr.nmda.ad.nmsu.edu> This being Halloween Week, you must be lurking out there somewhere... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From brett_connolly <@t> merck.com Wed Nov 2 09:15:01 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Nov 2 09:15:10 2011 Subject: [Histonet] RE: Luciferase IHC In-Reply-To: <040346FA7309BD439C327F97D4C4D69B0D78BF28@ISIS.fhcrc.org> References: <040346FA7309BD439C327F97D4C4D69B0D78BF28@ISIS.fhcrc.org> Message-ID: Julie, Are you looking to stain for firefly luciferase to confirm localization of an optical imaging probe? That's what I want to do, there are antibodies available from Lifespan and Biovision among others that are purported to work on FFPE sections ....I have yet to try them. You can check out this article, but they used a UK supplier. Bioluminescence imaging to monitor bladder cancer cell adhesion in vivo: a new approach to optimize a syngeneic, orthotopic, murine bladder cancer model. Jurczok A, Fornara P, S?ling A. BJU Int. 2008 Jan;101(1):120-4. Epub 2007 Sep 20. Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 . -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Randolph-Habecker, Julie Sent: Tuesday, November 01, 2011 6:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabecke@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From monettes <@t> mskcc.org Wed Nov 2 09:26:58 2011 From: monettes <@t> mskcc.org (monettes@mskcc.org) Date: Wed Nov 2 09:27:15 2011 Subject: [Histonet] mast cells for arteries In-Reply-To: <4937809CEF224CCE99B75C4F01EC4D5E@Patsyoffice> References: <4937809CEF224CCE99B75C4F01EC4D5E@Patsyoffice> Message-ID: Giemsa is what we use routinely for mast cell neoplasms and normal mast cells. For us it works equally well as T blue for diagnostic purposes. Our positive control is a cutaneous mast cell tumor (very common in out animal species), or skin biopsy with allergic dermatitis. Poorly granulated/degranulated mast cells won't stain with Giemsa or T Blue, and in those cases we use c-Kit IHC Best, Sebastien S?bastien Monette, DMV, MVSc, DACVP Tri-Institutional Laboratory of Comparative Pathology Center of Comparative Medicine and Pathology Memorial Sloan-Kettering Cancer Center Weill Cornell Medical College The Rockefeller University 1275 York Avenue, Box 270, New York NY 10065 Phone: 646-888-2420 Fax: 646-422-0139 Email MSKCC: monettes@mskcc.org Email WCMC: sem2010@med.cornell.edu Web site: http://www.mskcc.org/mskcc/html/92361.cfm -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Tuesday, November 01, 2011 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mast cells for arteries Does anyone have a go to stain for Mast Cells besides Tol Blue? We are trying to demonstrate the presence of mast cells in human arteries ffpe. Also, what would be a good positive control to use for mast cell staining, we used NH Skin and got maybe a few mast cells but I would like to use something more abundant with mast cells. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ===================================================================== Please note that this e-mail and any files transmitted with it may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer. From jhabecke <@t> fhcrc.org Wed Nov 2 10:43:12 2011 From: jhabecke <@t> fhcrc.org (Randolph-Habecker, Julie) Date: Wed Nov 2 10:43:22 2011 Subject: [Histonet] Luciferase IHC! In-Reply-To: <20111102134822.0E864A2992@dilbert.fhcrc.org> References: <20111102134822.0E864A2992@dilbert.fhcrc.org> Message-ID: <040346FA7309BD439C327F97D4C4D69B0D78C17D@ISIS.fhcrc.org> Thanks for the help! We actually tried antigen retrieval in Holy water in a pressure cooker for 10 minutes. I even brought in an old priest and a young priest to perform the staining but no luck! Sorry, no vendor for the holy water - the old priest makes his own. I enjoyed the laugh! This project has been hell! Thanks, Julie Message: 21 Date: Wed, 2 Nov 2011 08:46:28 -0500 From: "Beckham, Sharon" Subject: RE: [Histonet] Luciferase IHC To: "'O'Donnell, Bill'" , Kim Donadio , "Randolph-Habecker, Julie" , "histonet@lists.utsouthwestern.edu" Message-ID: <2C40E43D1F7A56408C4463FD245DDDF98AEE2C53@EXCHMB-02.stowers-institute.org> Content-Type: text/plain; charset="iso-8859-1" You know sometimes I wish these emails had a "LIKE" button on them. I found this quite funny and wanted to "LIKE" it. Too much facebook for me! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 02, 2011 8:19 AM To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out,,,, ? Sorry, I couldnt resist. ? ? ________________________________ From: "Randolph-Habecker, Julie" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabecke@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 3 *************************************** From Wanda.Smith <@t> HCAhealthcare.com Wed Nov 2 11:19:13 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Wed Nov 2 11:19:19 2011 Subject: [Histonet] SC Society of Histotechnology this weekend Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA273208994A@NADCWPMSGCMS03.hca.corpad.net> Hello to All, Just another reminder that our annual meeting of the SCSHT will be in Myrtle Beach, SC at the Marina Inn at Grande Dunes. Pre-registration is not required, you can register at the door! Good food, fun and fellowship and you will learn something too!!!!!!!!!!! Send me an email if you think you may be attending and have not registered. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From rory.pritchard001 <@t> gmail.com Wed Nov 2 11:23:37 2011 From: rory.pritchard001 <@t> gmail.com (Rory Pritchard) Date: Wed Nov 2 11:24:52 2011 Subject: [Histonet] CGRP immunopositive quantification Message-ID: <2DBF5936-A35C-4D4B-833A-DD2E35EFCAAE@gmail.com> Hi, I ran ICC on CGRP and the stain is very specific, it's just messy, containing cell bodies as well as dense fibers. I can't really just do a cell count and I don't want to use densitometry. Does anybody have any suggestions on what methods to use for quantifying CGRP fibers? Any help would be great. Thanks, Rory. From NMP <@t> stowers.org Wed Nov 2 11:31:33 2011 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Wed Nov 2 11:31:39 2011 Subject: [Histonet] RE: Luciferase IHC! In-Reply-To: <040346FA7309BD439C327F97D4C4D69B0D78C17D@ISIS.fhcrc.org> References: <20111102134822.0E864A2992@dilbert.fhcrc.org> <040346FA7309BD439C327F97D4C4D69B0D78C17D@ISIS.fhcrc.org> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF98B157A19@EXCHMB-02.stowers-institute.org> This is great. I've enjoyed the story line and witty comments and remarks. Thanks,Nanne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Randolph-Habecker, Julie Sent: Wednesday, November 02, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Luciferase IHC! Thanks for the help! We actually tried antigen retrieval in Holy water in a pressure cooker for 10 minutes. I even brought in an old priest and a young priest to perform the staining but no luck! Sorry, no vendor for the holy water - the old priest makes his own. I enjoyed the laugh! This project has been hell! Thanks, Julie Message: 21 Date: Wed, 2 Nov 2011 08:46:28 -0500 From: "Beckham, Sharon" Subject: RE: [Histonet] Luciferase IHC To: "'O'Donnell, Bill'" , Kim Donadio , "Randolph-Habecker, Julie" , "histonet@lists.utsouthwestern.edu" Message-ID: <2C40E43D1F7A56408C4463FD245DDDF98AEE2C53@EXCHMB-02.stowers-institute.org> Content-Type: text/plain; charset="iso-8859-1" You know sometimes I wish these emails had a "LIKE" button on them. I found this quite funny and wanted to "LIKE" it. Too much facebook for me! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 02, 2011 8:19 AM To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out,,,, ? Sorry, I couldnt resist. ? ? ________________________________ From: "Randolph-Habecker, Julie" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabecke@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 3 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Wed Nov 2 11:42:26 2011 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Nov 2 11:42:36 2011 Subject: [Histonet] Luciferase IHC/Holy water Message-ID: <8CE679589C4E314-1028-D091E@webmail-m013.sysops.aol.com> You can make your own holy water. You put regular water on a stove and boil the hell out of it! Mike Titford USA - Pathology Mobile AL From CIngles <@t> uwhealth.org Wed Nov 2 13:06:31 2011 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Nov 2 13:07:08 2011 Subject: [Histonet] Luciferase IHC References: <040346FA7309BD439C327F97D4C4D69B0D78BF28@ISIS.fhcrc.org> <1320192050.55176.YahooMailNeo@web112304.mail.gq1.yahoo.com> Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A780@UWHC-MAIL2.uwhis.hosp.wisc.edu> And it's not even Friday! Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kim Donadio Sent: Tue 11/1/2011 7:00 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out,,,, Sorry, I couldnt resist. From billodonnell <@t> catholichealth.net Wed Nov 2 13:23:50 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Nov 2 13:25:00 2011 Subject: [Histonet] Luciferase IHC/Holy water In-Reply-To: <8CE679589C4E314-1028-D091E@webmail-m013.sysops.aol.com> References: <8CE679589C4E314-1028-D091E@webmail-m013.sysops.aol.com> Message-ID: <4940DF6D1C5FDF48931B6966AAEF93952AC15A@chimsx08.CHI.catholichealth.net> Very similar to how I cook kidneys. (ever notice why there are no great Irish restaurants or cookbooks in the U.S.? William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Wednesday, November 02, 2011 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Luciferase IHC/Holy water You can make your own holy water. You put regular water on a stove and boil the hell out of it! Mike Titford USA - Pathology Mobile AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Wed Nov 2 13:29:52 2011 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Wed Nov 2 13:30:05 2011 Subject: [Histonet] out of office at noon today Message-ID: I will be out of the office starting 11/02/2011 and will not return until 11/07/2011. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. I will be in town. Thank you. From SWeaver <@t> tvmdl.tamu.edu Wed Nov 2 14:10:23 2011 From: SWeaver <@t> tvmdl.tamu.edu (Weaver, Stephanie) Date: Wed Nov 2 14:10:19 2011 Subject: [Histonet] deparaffinization with detergent Message-ID: I have seen a few comments over the years that mention deparaffinization of slides using hot soapy water instead of xylene. Does anyone have a reference they can direct me to where I can learn more about this or personal experience on how well it works and the logistics of making it work in the real world? Stephanie Weaver Texas Veterinary Medical Diagnostic Laboratory From nelsonrnch <@t> verizon.net Wed Nov 2 14:26:20 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Wed Nov 2 14:26:23 2011 Subject: [Histonet] FULL TIME CERTIFIED HISTOTECH WANTED Message-ID: <1320261980.84244.YahooMailNeo@web84502.mail.ne1.yahoo.com> New GI lab opening in Santa Monica Calif.? If you are looking to advance in your career to the next level and challenge yourself to new technology, than this might just be the perfect opportunity for you.?Some specifics are: ?Lead Tech position, full time / 1st shift available, but flexible on the hours. Must be self motivated and work well?independently. Knowledge of QA/QC? a plus, Grossing involved.? ? Position available immediately. Please submit resumes to e-mail below. Any questions you can call at the contact information below. THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANTS P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ? ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. From jkiernan <@t> uwo.ca Wed Nov 2 15:10:52 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Nov 2 15:10:56 2011 Subject: [Histonet] mast cells for arteries In-Reply-To: <4937809CEF224CCE99B75C4F01EC4D5E@Patsyoffice> References: <4937809CEF224CCE99B75C4F01EC4D5E@Patsyoffice> Message-ID: <7460eda355f9e.4eb16b8c@uwo.ca> If you don't want the with purple-red metachromasia, try neutral red, which gives an orange-red metachromasia and true red nuclei. John Kiernan Anatomy, UWO London, Canada = = = On 01/11/11, Patsy Ruegg wrote: > > Does anyone have a go to stain for Mast Cells besides Tol Blue? We are > trying to demonstrate the presence of mast cells in human arteries ffpe. > Also, what would be a good positive control to use for mast cell staining, > we used NH Skin and got maybe a few mast cells but I would like to use > something more abundant with mast cells. > > > > Best regards, > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email > pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that is > privileged & confidential within the meaning of applicable law. Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. If > you are NOT the intended recipient please contact the sender and dispose of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rosie_scrimizzi <@t> hotmail.com Wed Nov 2 15:14:23 2011 From: rosie_scrimizzi <@t> hotmail.com (Rosie Scrimizzi) Date: Wed Nov 2 15:14:33 2011 Subject: [Histonet] mast cells for arteries In-Reply-To: References: <4937809CEF224CCE99B75C4F01EC4D5E@Patsyoffice> Message-ID: Breast fibroadenoma is a great mast cell control Rosie Scrimizzi Oz Sent from my iPad On 03/11/2011, at 1:27 AM, monettes@mskcc.org wrote: > Giemsa is what we use routinely for mast cell neoplasms and normal mast cells. For us it works equally well as T blue for diagnostic purposes. Our positive control is a cutaneous mast cell tumor (very common in out animal species), or skin biopsy with allergic dermatitis. > > Poorly granulated/degranulated mast cells won't stain with Giemsa or T Blue, and in those cases we use c-Kit IHC > > Best, > Sebastien > > S?bastien Monette, DMV, MVSc, DACVP > Tri-Institutional Laboratory of Comparative Pathology > Center of Comparative Medicine and Pathology > Memorial Sloan-Kettering Cancer Center > Weill Cornell Medical College > The Rockefeller University > > 1275 York Avenue, Box 270, New York NY 10065 > Phone: 646-888-2420 > Fax: 646-422-0139 > Email MSKCC: monettes@mskcc.org > Email WCMC: sem2010@med.cornell.edu > Web site: http://www.mskcc.org/mskcc/html/92361.cfm > > > > -----Original Message----- > From: Patsy Ruegg [mailto:pruegg@ihctech.net] > Sent: Tuesday, November 01, 2011 1:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] mast cells for arteries > > Does anyone have a go to stain for Mast Cells besides Tol Blue? We are > trying to demonstrate the presence of mast cells in human arteries ffpe. > Also, what would be a good positive control to use for mast cell staining, > we used NH Skin and got maybe a few mast cells but I would like to use > something more abundant with mast cells. > > > > Best regards, > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that is > privileged & confidential within the meaning of applicable law. Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. If > you are NOT the intended recipient please contact the sender and dispose of > this e-mail as soon as possible. > > > > > > > ===================================================================== > > Please note that this e-mail and any files transmitted with it may be > privileged, confidential, and protected from disclosure under > applicable law. If the reader of this message is not the intended > recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > reading, dissemination, distribution, copying, or other use of this > communication or any of its attachments is strictly prohibited. If > you have received this communication in error, please notify the > sender immediately by replying to this message and deleting this > message, any attachments, and all copies and backups from your > computer. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Nov 2 15:25:52 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Nov 2 15:25:55 2011 Subject: [Histonet] deparaffinization with detergent In-Reply-To: References: Message-ID: <7650f63056541.4eb16f10@uwo.ca> See Buesa & Peshkov 2009 Annals of Diagnostic Pathology 13: 246-256. They thoroughly describe and discuss the method. John Kiernan Anatomy, UWO London, Canada = = = On 02/11/11, "Weaver, Stephanie" wrote: > > I have seen a few comments over the years that mention deparaffinization of slides using hot soapy water instead of xylene. Does anyone have a reference they can direct me to where I can learn more about this or personal experience on how well it works and the logistics of making it work in the real world? > > Stephanie Weaver > Texas Veterinary Medical Diagnostic Laboratory > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From pruegg <@t> ihctech.net Wed Nov 2 15:52:24 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 2 15:52:30 2011 Subject: [Histonet] DAB Immuno and alcian blue counter staining In-Reply-To: <9DF797D618351549B984596F01A1FE1D01FCC8D2@murmx.mcri.edu.au> References: <1320076911.80808.YahooMailNeo@web112303.mail.gq1.yahoo.com><9DF797D618351549B984596F01A1FE1D01FCC75D@murmx.mcri.edu.au> <9DF797D618351549B984596F01A1FE1D01FCC8D2@murmx.mcri.edu.au> Message-ID: Try decreasing the alcian blue concentration and staining time. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniela Bodemer Sent: Tuesday, November 01, 2011 8:47 PM To: Histonet@lists.utsouthwestern.edu Cc: Susan Lapthorne Subject: [Histonet] DAB Immuno and alcian blue counter staining Hi all, Is it possible to do an immuno with DAB detection and counterstain it with alcian blue at the end to detect Goblets cells? I've tried it and the DAB disappears under the staining. Thanks, Daniela ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Nov 2 16:05:26 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 2 16:05:32 2011 Subject: [Histonet] Must be Monday... In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DFB81@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B051DFB81@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Yea what is up with that all of a sudden my Dako autostainer is an hour behind, it must have switched to the dst when we used to do that and now we do that later, right, so I will have to wait a while for it to catch up, when does that happen now days? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 6:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Nov 2 16:07:15 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Nov 2 16:07:21 2011 Subject: [Histonet] Must be Monday... In-Reply-To: References: <4D14F0FC9316DD41972D5F03C070908B051DFB81@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408415721F4@CHEXCMS10.one.ads.che.org> Next Sunday my dear.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Wednesday, November 02, 2011 17:05 To: 'Breeden, Sara'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Must be Monday... Yea what is up with that all of a sudden my Dako autostainer is an hour behind, it must have switched to the dst when we used to do that and now we do that later, right, so I will have to wait a while for it to catch up, when does that happen now days? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 6:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From pruegg <@t> ihctech.net Wed Nov 2 16:08:20 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 2 16:08:26 2011 Subject: [Histonet] RE: Must be Monday... In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39127401B441@IBMB7Exchange.digestivespecialists.com> References: <4D14F0FC9316DD41972D5F03C070908B051DFB81@nmdamailsvr.nmda.ad.nmsu.edu> <5A2BD13465E061429D6455C8D6B40E39127401B441@IBMB7Exchange.digestivespecialists.com> Message-ID: <163D5FC59C5C4F8A9F90E9589B2157C7@prueggihctechlt> Yep I have an older Dako and I forget that it changes to dst at the old time, well the new change should come up this weekend Nov 6th so everything should be back on schedule after that????? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, October 31, 2011 6:40 AM To: 'Breeden, Sara'; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Must be Monday... That's a hoot Sally! I don't think that DST of old was even this past weekend. For the past 3 years my processor changed on the DST of old and the powers that be told me it was impossible because that wasn't programmed into my processor's data. This year I forgot about it and it didn't change on the old date. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 8:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Wed Nov 2 16:08:35 2011 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Wed Nov 2 16:08:40 2011 Subject: [Histonet] General orientation checklist Message-ID: Does anyone have a general orientation checklist for new hires that they would be willing to share . Thanks in advance Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From dmburns9 <@t> gmail.com Wed Nov 2 17:06:48 2011 From: dmburns9 <@t> gmail.com (Douglas M Burns) Date: Wed Nov 2 17:06:52 2011 Subject: [Histonet] problem with brain sections falling off subbed slides Message-ID: Hello, I am new to working with brain, and I am having a bad problem with both thin and thick rat brain sections sliding off of subbed slides. We tried commercial treated slides first, but then moved on to subbing them. The subbing was done with gelatin according to a printed protocol, and I can see the film on the dried slide. When kept horizontal they seem okay, but as soon as they are put into a slide holder for washing or anything, they begin to slide off or even move around. After a short while, many/most of mysteriously vanished (into the wash solution). Our slices are sliced by cryostat from a rat brain that has been fixed with 4% paraformaldehyde and 30% sucrose. The slice comes right off the cryostat, and then I m ove it onto a warm slide. (Perhaps they are not warm enough?? I have seen others blow on them to warm them.) Slides destined for Cresyl violet staining were dried for 3 days at room temperature before being moved. It is the Cresyl violet protocol that causes the most trouble, but I am seeing some problems with any type of large volume wash. At this point, I don't have enough experience with this specific tissue and our protocols to be able to tell for sure what is happening. Thus, I am entirely open to advice - any advice that might help with the problem. Thank you ------------- Doug Burns, Kansas City From jnocito <@t> satx.rr.com Wed Nov 2 18:26:04 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Nov 2 18:26:28 2011 Subject: [Histonet] RE: Luciferase IHC! In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF98B157A19@EXCHMB-02.stowers-institute.org> References: <20111102134822.0E864A2992@dilbert.fhcrc.org><040346FA7309BD439C327F97D4C4D69B0D78C17D@ISIS.fhcrc.org> <2C40E43D1F7A56408C4463FD245DDDF98B157A19@EXCHMB-02.stowers-institute.org> Message-ID: <9679D6A078A74F8A94D077B47D7DCFBC@JoePC> I'm here. I was asking my Priest the steps to perform an exorcism, although the Catholic Church doesn't perform these rituals any longer. I have all the materials, readings and "exorcism paraphernalia" Just let me know where and when. Just make sure there is a good bottle of Scotch available, just in case all else fails. JTT ----- Original Message ----- From: "Marsh, Nannette" To: "'Randolph-Habecker, Julie'" ; Sent: Wednesday, November 02, 2011 11:31 AM Subject: [Histonet] RE: Luciferase IHC! This is great. I've enjoyed the story line and witty comments and remarks. Thanks,Nanne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Randolph-Habecker, Julie Sent: Wednesday, November 02, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Luciferase IHC! Thanks for the help! We actually tried antigen retrieval in Holy water in a pressure cooker for 10 minutes. I even brought in an old priest and a young priest to perform the staining but no luck! Sorry, no vendor for the holy water - the old priest makes his own. I enjoyed the laugh! This project has been hell! Thanks, Julie Message: 21 Date: Wed, 2 Nov 2011 08:46:28 -0500 From: "Beckham, Sharon" Subject: RE: [Histonet] Luciferase IHC To: "'O'Donnell, Bill'" , Kim Donadio , "Randolph-Habecker, Julie" , "histonet@lists.utsouthwestern.edu" Message-ID: <2C40E43D1F7A56408C4463FD245DDDF98AEE2C53@EXCHMB-02.stowers-institute.org> Content-Type: text/plain; charset="iso-8859-1" You know sometimes I wish these emails had a "LIKE" button on them. I found this quite funny and wanted to "LIKE" it. Too much facebook for me! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 02, 2011 8:19 AM To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out,,,, Sorry, I couldnt resist. ________________________________ From: "Randolph-Habecker, Julie" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabecke@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 3 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Nov 2 18:28:36 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Nov 2 18:28:59 2011 Subject: [Histonet] Where are you, JTT? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DFB9E@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B051DFB9E@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <160C3A4151FB4918A08E0647AC8299B3@JoePC> My son is heavily in high school activities now. I have to drive him to all these school events. I know I'll probably pay for this later down the road, but I'm actually pushing him to get his driver's permit so he can drive. by time we get home, it's time for my beauty sleep. If you saw me in Cinci, you'd understand that I need all the help I can get. JTT ----- Original Message ----- From: "Breeden, Sara" To: Sent: Wednesday, November 02, 2011 9:08 AM Subject: [Histonet] Where are you, JTT? This being Halloween Week, you must be lurking out there somewhere... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diane.Tokugawa <@t> kp.org Wed Nov 2 18:35:29 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Wed Nov 2 18:35:44 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 11/02/2011 and will not return until 11/10/2011. Note: For Cytology issues, please call Molly at 8-421-5487, Eric at 8-421-5405, or Wanda 8-421-5426 For Histology issues, please call Mario at 8-421-4961, general histology lab 8-421- 5408, Kiran at 8-421-5404, or Wanda at 8-421-5426. From one_angel_secret <@t> yahoo.com Wed Nov 2 19:36:52 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Nov 2 19:36:56 2011 Subject: [Histonet] RE: Luciferase IHC! In-Reply-To: <9679D6A078A74F8A94D077B47D7DCFBC@JoePC> References: <20111102134822.0E864A2992@dilbert.fhcrc.org><040346FA7309BD439C327F97D4C4D69B0D78C17D@ISIS.fhcrc.org> <2C40E43D1F7A56408C4463FD245DDDF98B157A19@EXCHMB-02.stowers-institute.org> <9679D6A078A74F8A94D077B47D7DCFBC@JoePC> Message-ID: <1320280612.80334.YahooMailNeo@web112312.mail.gq1.yahoo.com> a good exorcism is hard to come by. ? Good scotch, I think I can provide. Ive looked and looked and the cure or diagnosis for this is hard to find. ? All the nuns agreed till de-cloaking was mentioned ? :D ________________________________ From: Joe Nocito To: "Marsh, Nannette" ; "'Randolph-Habecker, Julie'" ; histonet@lists.utsouthwestern.edu Sent: Wednesday, November 2, 2011 7:26 PM Subject: Re: [Histonet] RE: Luciferase IHC! I'm here.? I was asking my Priest the steps to perform an exorcism, although the Catholic Church doesn't perform these rituals any longer. I have all the materials, readings and "exorcism paraphernalia" Just let me know where and when. Just make sure there is a good bottle of Scotch available, just in case all else fails. JTT ----- Original Message ----- From: "Marsh, Nannette" To: "'Randolph-Habecker, Julie'" ; Sent: Wednesday, November 02, 2011 11:31 AM Subject: [Histonet] RE: Luciferase IHC! This is great.? I've enjoyed the story line and witty comments and remarks. Thanks,Nanne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Randolph-Habecker, Julie Sent: Wednesday, November 02, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Luciferase IHC! Thanks for the help! We actually tried antigen retrieval in Holy water in a pressure cooker for 10 minutes. I even brought in an old priest and a young priest to perform the staining but no luck! Sorry, no vendor for the holy water - the old priest makes his own. I enjoyed the laugh! This project has been hell! Thanks, Julie Message: 21 Date: Wed, 2 Nov 2011 08:46:28 -0500 From: "Beckham, Sharon" Subject: RE: [Histonet] Luciferase IHC To: "'O'Donnell, Bill'" , Kim Donadio , "Randolph-Habecker, Julie" , "histonet@lists.utsouthwestern.edu" Message-ID: <2C40E43D1F7A56408C4463FD245DDDF98AEE2C53@EXCHMB-02.stowers-institute.org> Content-Type: text/plain; charset="iso-8859-1" You know sometimes I wish these emails had a "LIKE" button on them.? I found this quite funny and wanted to "LIKE" it.? Too much facebook for me! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, November 02, 2011 8:19 AM To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Luciferase IHC Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, November 01, 2011 7:01 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in holy water, then all H311 will break out,,,, Sorry, I couldnt resist. ________________________________ From: "Randolph-Habecker, Julie" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 1, 2011 6:49 PM Subject: [Histonet] Luciferase IHC Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use. Thanks!! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabecke@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 3 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Nov 2 22:19:22 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Nov 2 22:19:27 2011 Subject: [Histonet] problem with brain sections falling off subbed slides In-Reply-To: References: Message-ID: <7640d27b80511.4eb1cffa@uwo.ca> Gelatin alone is not an adhesive because it dissolves in water. Chrome alum-gelatin is an excellent adhesive for sections and costs almost nothing to make. Subbed slides are one coated with chrome gelatin, dried and stored. The following web link shows a 1999 publication giving the rationale and methods for a variety of section adhesives. http://publish.uwo.ca/~jkiernan/adhesivs.htm John Kiernan Anatomy,UWO London, Canada = = = On 02/11/11, Douglas M Burns wrote: > > Hello, > > I am new to working with brain, and I am having a bad problem with > both thin and thick rat brain sections sliding off of subbed slides. > > We tried commercial treated slides first, but then moved on to > subbing them. The subbing was done with gelatin according to a printed > protocol, and I can see the film on the dried slide. When kept horizontal > they seem okay, but as soon as they are put into a slide holder for washing > or anything, they begin to slide off or even move around. After a short > while, many/most of mysteriously vanished (into the wash solution). > > Our slices are sliced by cryostat from a rat brain that has been > fixed with 4% paraformaldehyde and 30% sucrose. The slice comes right off > the cryostat, and then I m ove it onto a warm slide. (Perhaps they are not > warm enough?? I have seen others blow on them to warm them.) Slides > destined for Cresyl violet staining were dried for 3 days at room > temperature before being moved. > > It is the Cresyl violet protocol that causes the most trouble, but I > am seeing some problems with any type of large volume wash. At this point, > I don't have enough experience with this specific tissue and our protocols > to be able to tell for sure what is happening. Thus, I am entirely open to > advice - any advice that might help with the problem. > > Thank you ------------- Doug Burns, Kansas City > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From k84as <@t> yahoo.com Wed Nov 2 23:41:44 2011 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Wed Nov 2 23:42:42 2011 Subject: [Histonet] (no subject) Message-ID: <1320295304.92493.yint-ygo-j2me@web112612.mail.gq1.yahoo.com> http://blog.ergle.org/wp-content/plugins/extended-comment-options/markehjf.htm From one_angel_secret <@t> yahoo.com Thu Nov 3 07:46:48 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Nov 3 07:47:57 2011 Subject: [Histonet] problem with brain sections falling off subbed slides In-Reply-To: <7640d27b80511.4eb1cffa@uwo.ca> References: <7640d27b80511.4eb1cffa@uwo.ca> Message-ID: <1320324408.21358.YahooMailNeo@web112320.mail.gq1.yahoo.com> Good morning. I see you mention only part of your protocol so I am not sure if you are going directly to the stain? This procedure seems close to what you are working with, it has a few tips. Hope this helps. ? Procedure: 1. Mount frozen or vibratome sections on gelatin coated or positive charged plus slides. Air dry sections or bake slides on slide warmer overnight. 2. Place slides directly into 1:1 alcohol/chloroform overnight and then rehydrate through 100% and 95 % alcohol to distilled water. DON?T put frozen sections directly to water otherwise they will come off the slides.This is called de-fat step that will reduce background fat staining. 3. Stain in 0.1% cresyl violet solution for 5-10 minutes. Notes:Staining in warmed cresyl violet solution (warm up in 37-50 ?C oven) can improve penetration and enhancing even staining. It is particularly beneficial for thicker (30 um) sections. 4. Rinse quickly in distilled water. 5. Differentiate in 95% ethyl alcohol for 2-30 minutes and check microscopically for best result. 6. Dehydrate in 100% alcohol 2x5 min. 7. Clear in xylene 2x5 min. 8. Mount with permanent mountingmedium. ? http://www.ihcworld.com/_protocols/special_stains/nissl-frozen-section.htm ? Kim Donadio ________________________________ From: John Kiernan To: Douglas M Burns ; histonet@lists.utsouthwestern.edu Sent: Wednesday, November 2, 2011 11:19 PM Subject: Re: [Histonet] problem with brain sections falling off subbed slides Gelatin alone is not an adhesive because it dissolves in water. Chrome alum-gelatin is an excellent adhesive for sections and costs almost nothing to make. Subbed slides are one coated with chrome gelatin, dried and stored. The following web link shows a 1999 publication giving the rationale and methods for a variety of section adhesives. ? ? http://publish.uwo.ca/~jkiernan/adhesivs.htm John Kiernan Anatomy,UWO London, Canada = = = On 02/11/11, Douglas M Burns wrote: > > Hello, > >? ? ? I am new to working with brain, and I am having a bad problem with > both thin and thick rat brain sections sliding off of subbed slides. > >? ? ? We tried commercial treated slides first, but then? moved on to > subbing them. The subbing was done with gelatin according to a printed > protocol, and I can see the film on the dried slide. When kept horizontal > they seem okay, but as soon as they are put into a slide holder for washing > or anything, they begin to slide off or even move around. After a short > while, many/most of mysteriously vanished (into the wash solution). > >? ? ? Our slices are sliced by cryostat from a rat brain that has been > fixed with 4% paraformaldehyde and 30% sucrose. The slice comes right off > the cryostat, and then I m ove it onto a warm slide. (Perhaps they are not > warm enough?? I have seen others blow on them to warm them.) Slides > destined for Cresyl violet staining were dried for 3 days at room > temperature before being moved. > >? ? ? ? It is the Cresyl violet protocol that causes the most trouble, but I > am seeing some problems with any type of large volume wash. At this point, > I don't have enough experience with this specific tissue and our protocols > to be able to tell for sure what is happening. Thus, I am entirely open to > advice - any advice that might help with the problem. > >? ? ? ? ? ? ? ? ? ? ? Thank? you? ? -------------? ? Doug Burns, Kansas City > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From making <@t> ufl.edu Thu Nov 3 08:04:25 2011 From: making <@t> ufl.edu (King,Michael A) Date: Thu Nov 3 08:04:30 2011 Subject: [Histonet] problem with brain sections falling off subbed slides Message-ID: <3b0b37ec1a928aab52f3d9081609fa57@ufl.edu> Apart from Dr. Kiernan's recommendation, you can try putting your mounted slides in fixative for a few minutes, we've found this to work with gelatin-only coated slides, or chrome-alum gelatin subbed slides with thick brain sections. If you're trying to rescue material already on slides, dip briefly in warm 1% gelatin, air dry, fix for a few minutes in 10% NBF, and proceed to counterstaining. ----------- Message: 14 Date: Wed, 2 Nov 2011 17:06:48 -0500 From: Douglas M Burns Subject: [Histonet] problem with brain sections falling off subbed slides Hello, I am new to working with brain, and I am having a bad problem with both thin and thick rat brain sections sliding off of subbed slides... From Jennifer.Bull <@t> northwestpathology.com Thu Nov 3 09:42:49 2011 From: Jennifer.Bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Thu Nov 3 09:42:56 2011 Subject: [Histonet] Specimen Tracking for courier Message-ID: <85760CECEC18444BB95F26D5E88DAEAA22ED9467DF@hinet2.hinet.org> I'm curious what methods other labs are using to track specimens that couriers pick up from clinic locations to ensure safe delivery to the lab. We currently utilize a logbook at the clinic site that the courier signs when they take the specimen but I have heard there are barcoded systems available out there as well. What works? What doesn't? Appreciate your feedback! Jenny mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- From cpyse <@t> x-celllab.com Thu Nov 3 10:13:02 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Thu Nov 3 10:13:02 2011 Subject: [Histonet] Specimen Tracking for courier In-Reply-To: <85760CECEC18444BB95F26D5E88DAEAA22ED9467DF@hinet2.hinet.org> References: <85760CECEC18444BB95F26D5E88DAEAA22ED9467DF@hinet2.hinet.org> Message-ID: <001501cc9a3b$15135c00$3f3a1400$@com> I would be interested in everyone responses. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manger X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bull, Jennifer L. Sent: Thursday, November 03, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Tracking for courier I'm curious what methods other labs are using to track specimens that couriers pick up from clinic locations to ensure safe delivery to the lab. We currently utilize a logbook at the clinic site that the courier signs when they take the specimen but I have heard there are barcoded systems available out there as well. What works? What doesn't? Appreciate your feedback! Jenny mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Thu Nov 3 10:23:23 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Nov 3 10:23:34 2011 Subject: [Histonet] Specimen Tracking for courier In-Reply-To: <001501cc9a3b$15135c00$3f3a1400$@com> References: <85760CECEC18444BB95F26D5E88DAEAA22ED9467DF@hinet2.hinet.org> <001501cc9a3b$15135c00$3f3a1400$@com> Message-ID: Our lab as a whole uses the Gajema Specimen Transport And Tracking System for all courier deliveries Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Thursday, November 03, 2011 11:13 AM To: 'Bull, Jennifer L.'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Specimen Tracking for courier I would be interested in everyone responses. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manger X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bull, Jennifer L. Sent: Thursday, November 03, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Tracking for courier I'm curious what methods other labs are using to track specimens that couriers pick up from clinic locations to ensure safe delivery to the lab. We currently utilize a logbook at the clinic site that the courier signs when they take the specimen but I have heard there are barcoded systems available out there as well. What works? What doesn't? Appreciate your feedback! Jenny mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From dcampbell <@t> trianglebiomedical.com Thu Nov 3 10:27:15 2011 From: dcampbell <@t> trianglebiomedical.com (Dustin Paul Campbell) Date: Thu Nov 3 10:27:22 2011 Subject: [Histonet] Specimen Tracking for courier In-Reply-To: <001501cc9a3b$15135c00$3f3a1400$@com> References: <85760CECEC18444BB95F26D5E88DAEAA22ED9467DF@hinet2.hinet.org> <001501cc9a3b$15135c00$3f3a1400$@com> Message-ID: <30D679350711B041B577E61FCFC90B68C5810C@tbssbs.TBS.local> Jenny, I know Triangle Urology Associates in NC is using TBS' SHUR/Mark-TVT tracking and verification system and love it! If you would like additional info I will be happy to get it for you? Dustin Campbell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Thursday, November 03, 2011 11:13 AM To: 'Bull, Jennifer L.'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Specimen Tracking for courier I would be interested in everyone responses. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manger X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bull, Jennifer L. Sent: Thursday, November 03, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Tracking for courier I'm curious what methods other labs are using to track specimens that couriers pick up from clinic locations to ensure safe delivery to the lab. We currently utilize a logbook at the clinic site that the courier signs when they take the specimen but I have heard there are barcoded systems available out there as well. What works? What doesn't? Appreciate your feedback! Jenny mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy_McLaughlin <@t> cooley-dickinson.org Thu Nov 3 12:03:44 2011 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Thu Nov 3 12:04:37 2011 Subject: [Histonet] Staffing to work ratios Message-ID: Hi, I know this subject has been discussed before, but I'm having trouble finding the info I need from the archives. Would anyone know where I can find the work to staffing ratios for a Histology laboratory? Our volume was ~9750 surgical cases (2010). The majority of them are biopsies (GI, GYN, skin, etc) but we do some larger complex cases. We had 2569 billable IHC tests, 254 billable group I special stains, 722 billable group II special stains. #blocks: 23,380 # H&E slides : 49,524 Thank you for your help! Stacy McLaughlin, HT(ASCP) Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 (413)582-2019 Stacy_McLaughlin@Cooley-Dickinson.org From Donna.Hunter <@t> Northside.com Thu Nov 3 12:22:02 2011 From: Donna.Hunter <@t> Northside.com (Donna Hunter) Date: Thu Nov 3 12:22:18 2011 Subject: [Histonet] Names of Hospitals with Histology in Alabama Message-ID: <731941C266951A47BEF11E5EFAAED9C906D27B87@nsmvexch01.northside.local> Fishing for some Names in Alabama that have Histology Labs? Thanks Donna Hunter HTII CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From Diana.Harris <@t> viha.ca Thu Nov 3 12:34:03 2011 From: Diana.Harris <@t> viha.ca (Harris, Diana) Date: Thu Nov 3 12:34:09 2011 Subject: [Histonet] Training and Competency Assessment for H&E Slide Review Message-ID: Any suggestions for creating a Training and Competency (T&C) Assessment for H&E slide review? We currently QC all H&E slides macroscopically and 15% microscopically. I would like to have all Histotechs trained and competent to QC H&E slides. Has anyone gone thru this process? Thanks Diana Harris QC & Method Development Technologist Royal Jubilee Hospital Victoria, BC Canada From joelleweaver <@t> hotmail.com Thu Nov 3 12:42:53 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Thu Nov 3 12:42:57 2011 Subject: [Histonet] Training and Competency Assessment for H&E Slide Review Message-ID: Any certified histologist has gone through this, but the NSH has good resources for this. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Harris Diana Date: Thu, 3 Nov 2011 17:34:03 To: Subject: [Histonet] Training and Competency Assessment for H&E Slide Review Any suggestions for creating a Training and Competency (T&C) Assessment for H&E slide review?? We currently QC all H&E slides macroscopically and 15% microscopically.? I would like to have all Histotechs trained and competent to QC H&E slides.? Has anyone gone thru this process? Thanks Diana Harris QC & Method Development Technologist Royal Jubilee Hospital Victoria, BC? Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Nov 3 14:05:29 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Nov 3 14:05:33 2011 Subject: [Histonet] General orientation checklist In-Reply-To: References: Message-ID: If you still need help with this, I have done this project before a couple of times and put it online via CAP's website and coursebuilder. You can let me know at my email address.Thanks Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > From: Allison_Scott@hchd.tmc.edu > To: histonet@lists.utsouthwestern.edu > Date: Wed, 2 Nov 2011 21:08:35 +0000 > Subject: [Histonet] General orientation checklist > > Does anyone have a general orientation checklist for new hires that they would be willing to share . Thanks in advance > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas 77026 > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Thu Nov 3 14:08:09 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Nov 3 14:08:12 2011 Subject: [Histonet] Brain sections... Message-ID: <1320347289.96989.YahooMailNeo@web39411.mail.mud.yahoo.com> This works if you are doing traditional specials--never worked out if it affected IHC.? We used it for autopsy brained -called it "double cook"--and you can't scratch the tissue off with a knife after they're handled like this. ? Dry your slides in low heat the way you always would.? Then cool completely.? Heat them again til the wax melts and the slide is up to the higher temp (about 10 minutes).? Cool and handle as usual. ? Something about the expanding and contracting of going in and out of the heat twice 'snuggles' the tissue onto the slide.? Cheryl Kerry, HT(ASCP) Full Staff Inc.? From JWeems <@t> sjha.org Thu Nov 3 14:21:55 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Nov 3 14:22:08 2011 Subject: [Histonet] Brain sections... In-Reply-To: <1320347289.96989.YahooMailNeo@web39411.mail.mud.yahoo.com> References: <1320347289.96989.YahooMailNeo@web39411.mail.mud.yahoo.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640844197066@CHEXCMS10.one.ads.che.org> And I've always found that letting them air dry overnight before drying in the oven works well too. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Thursday, November 03, 2011 15:08 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Brain sections... This works if you are doing traditional specials--never worked out if it affected IHC.? We used it for autopsy brained -called it "double cook"--and you can't scratch the tissue off with a knife after they're handled like this. ? Dry your slides in low heat the way you always would.? Then cool completely.? Heat them again til the wax melts and the slide is up to the higher temp (about 10 minutes).? Cool and handle as usual. ? Something about the expanding and contracting of going in and out of the heat twice 'snuggles' the tissue onto the slide.? Cheryl Kerry, HT(ASCP) Full Staff Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From crochieresteve <@t> aol.com Fri Nov 4 02:21:19 2011 From: crochieresteve <@t> aol.com (Steven) Date: Fri Nov 4 02:21:39 2011 Subject: [Histonet] Histo Tech Wanted Message-ID: <8CE68D97AFFDF8F-18C-4F49E@webmail-d072.sysops.aol.com> Immediate opening for an experienced histology technician in a private dermatolgy laboratory in Springfield, MA. Full time, Monday - Friday, day shift. All aspects of routine histology. Frozen section experience a plus. Competitive salary and attractive benefit package, Contact: Shannon Page or Steve Crochiere New England Dermatology & Laser Center 3455 Main St. Springfield, MA 01107 (413) 733-9600 SPage@NEDLC.com SCrochiere@NEDLC.com From TMcNemar <@t> lmhealth.org Fri Nov 4 05:57:32 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Nov 4 05:57:35 2011 Subject: [Histonet] Regarding MOHS.... Message-ID: Hello all, I have some questions for the community sized hospital that are doing MOHS. I would appreciated any input on the following questions: Do you have people specifically dedicated to performing MOHS? About how many specimens per patient do you receive? Are MOHS procedures performed STAT? Do you have more than one person working on MOHS (one to cut & one to stain)? Again, thanks in advance for you input. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From nelsonrnch <@t> verizon.net Fri Nov 4 06:11:35 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Fri Nov 4 06:11:37 2011 Subject: [Histonet] Statistical Information for GI Bx Message-ID: <1320405095.62735.YahooMailNeo@web84515.mail.ne1.yahoo.com> Does anyone know if and where I can find any statistical information on the average?number of?GI biopsies taken per patient? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ? ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. From Marilyn.A.Weiss <@t> kp.org Fri Nov 4 06:28:29 2011 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri Nov 4 06:28:44 2011 Subject: [Histonet] Re: Histonet Digest, Vol 96, Issue 6 In-Reply-To: <201111031703.pA3H36pq029512@cnndcsmrp116.nndc.kp.org> References: <201111031703.pA3H36pq029512@cnndcsmrp116.nndc.kp.org> Message-ID: we currently use a form called Pactrak and then place it into the LMS system for tracking when things go out of our lab. these go usually to outlying areas. When specimens are brought to the lab we have paperwork that we call transmittal sheets. They have to be signed off by the person that receives them and then again verified once it goes to the lab. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. histonet-request@lists.utsouthwestern.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 11/03/2011 10:03 AM Please respond to histonet@lists.utsouthwestern.edu To histonet@lists.utsouthwestern.edu cc Subject Histonet Digest, Vol 96, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. problem with brain sections falling off subbed slides (King,Michael A) 2. Specimen Tracking for courier (Bull, Jennifer L.) 3. RE: Specimen Tracking for courier (Cynthia Pyse) 4. RE: Specimen Tracking for courier (Houston, Ronald) 5. RE: Specimen Tracking for courier (Dustin Paul Campbell) ---------------------------------------------------------------------- Message: 1 Date: Thu, 03 Nov 2011 09:04:25 -0400 From: "King,Michael A" Subject: [Histonet] problem with brain sections falling off subbed slides To: Message-ID: <3b0b37ec1a928aab52f3d9081609fa57@ufl.edu> Content-Type: text/plain; charset=UTF-8; format=flowed Apart from Dr. Kiernan's recommendation, you can try putting your mounted slides in fixative for a few minutes, we've found this to work with gelatin-only coated slides, or chrome-alum gelatin subbed slides with thick brain sections. If you're trying to rescue material already on slides, dip briefly in warm 1% gelatin, air dry, fix for a few minutes in 10% NBF, and proceed to counterstaining. ----------- Message: 14 Date: Wed, 2 Nov 2011 17:06:48 -0500 From: Douglas M Burns Subject: [Histonet] problem with brain sections falling off subbed slides Hello, I am new to working with brain, and I am having a bad problem with both thin and thick rat brain sections sliding off of subbed slides... ------------------------------ Message: 2 Date: Thu, 3 Nov 2011 07:42:49 -0700 From: "Bull, Jennifer L." Subject: [Histonet] Specimen Tracking for courier To: "histonet@lists.utsouthwestern.edu" Message-ID: <85760CECEC18444BB95F26D5E88DAEAA22ED9467DF@hinet2.hinet.org> Content-Type: text/plain; charset=us-ascii I'm curious what methods other labs are using to track specimens that couriers pick up from clinic locations to ensure safe delivery to the lab. We currently utilize a logbook at the clinic site that the courier signs when they take the specimen but I have heard there are barcoded systems available out there as well. What works? What doesn't? Appreciate your feedback! Jenny mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- ------------------------------ Message: 3 Date: Thu, 3 Nov 2011 11:13:02 -0400 From: "Cynthia Pyse" Subject: RE: [Histonet] Specimen Tracking for courier To: "'Bull, Jennifer L.'" , Message-ID: <001501cc9a3b$15135c00$3f3a1400$@com> Content-Type: text/plain; charset="us-ascii" I would be interested in everyone responses. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manger X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bull, Jennifer L. Sent: Thursday, November 03, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Tracking for courier I'm curious what methods other labs are using to track specimens that couriers pick up from clinic locations to ensure safe delivery to the lab. We currently utilize a logbook at the clinic site that the courier signs when they take the specimen but I have heard there are barcoded systems available out there as well. What works? What doesn't? Appreciate your feedback! Jenny mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 3 Nov 2011 15:23:23 +0000 From: "Houston, Ronald" Subject: RE: [Histonet] Specimen Tracking for courier To: 'Cynthia Pyse' , "'Bull, Jennifer L.'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Our lab as a whole uses the Gajema Specimen Transport And Tracking System for all courier deliveries Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Thursday, November 03, 2011 11:13 AM To: 'Bull, Jennifer L.'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Specimen Tracking for courier I would be interested in everyone responses. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manger X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bull, Jennifer L. Sent: Thursday, November 03, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Tracking for courier I'm curious what methods other labs are using to track specimens that couriers pick up from clinic locations to ensure safe delivery to the lab. We currently utilize a logbook at the clinic site that the courier signs when they take the specimen but I have heard there are barcoded systems available out there as well. What works? What doesn't? Appreciate your feedback! Jenny mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 5 Date: Thu, 3 Nov 2011 11:27:15 -0400 From: "Dustin Paul Campbell" Subject: RE: [Histonet] Specimen Tracking for courier To: "Cynthia Pyse" , Message-ID: <30D679350711B041B577E61FCFC90B68C5810C@tbssbs.TBS.local> Content-Type: text/plain; charset="us-ascii" Jenny, I know Triangle Urology Associates in NC is using TBS' SHUR/Mark-TVT tracking and verification system and love it! If you would like additional info I will be happy to get it for you? Dustin Campbell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Thursday, November 03, 2011 11:13 AM To: 'Bull, Jennifer L.'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Specimen Tracking for courier I would be interested in everyone responses. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manger X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bull, Jennifer L. Sent: Thursday, November 03, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Tracking for courier I'm curious what methods other labs are using to track specimens that couriers pick up from clinic locations to ensure safe delivery to the lab. We currently utilize a logbook at the clinic site that the courier signs when they take the specimen but I have heard there are barcoded systems available out there as well. What works? What doesn't? Appreciate your feedback! Jenny mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 6 *************************************** From flnails <@t> texaschildrens.org Fri Nov 4 07:59:29 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Nov 4 07:59:40 2011 Subject: [Histonet] Statistical Information for GI Bx In-Reply-To: <1320405095.62735.YahooMailNeo@web84515.mail.ne1.yahoo.com> References: <1320405095.62735.YahooMailNeo@web84515.mail.ne1.yahoo.com> Message-ID: The patient condition should dictate what is taken, so the number will vary. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SHANE NELSON Sent: Friday, November 04, 2011 6:12 AM To: Histonet Subject: [Histonet] Statistical Information for GI Bx Does anyone know if and where I can find any statistical information on the average?number of?GI biopsies taken per patient? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ? ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From amber.mckenzie <@t> gastrodocs.net Fri Nov 4 08:28:30 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Fri Nov 4 08:27:33 2011 Subject: [Histonet] Statistical Information for GI Bx In-Reply-To: References: <1320405095.62735.YahooMailNeo@web84515.mail.ne1.yahoo.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BC0616DC@JERRY.Gia.com> Our Peds Dr takes anywhere from 6 to 13 bx's, depending on if it's a lower or upper GI or a double. Our other dr's take 0-6 bx's per patient depending on if the patient has problems, if the dr see's something he wants to bx, etc...or zero of everything looks normal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, November 04, 2011 7:59 AM To: 'SHANE NELSON'; Histonet Subject: RE: [Histonet] Statistical Information for GI Bx The patient condition should dictate what is taken, so the number will vary. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SHANE NELSON Sent: Friday, November 04, 2011 6:12 AM To: Histonet Subject: [Histonet] Statistical Information for GI Bx Does anyone know if and where I can find any statistical information on the average?number of?GI biopsies taken per patient? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ? ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Fri Nov 4 08:49:50 2011 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Fri Nov 4 08:49:58 2011 Subject: [Histonet] low profile vs. hi profile blades Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B8858@PEITHA.wad.pa-ucl.com> Hi- Please educate me on this. There seems to be a lot of personal opinion involved in regards to which type of blade to use. Thanks for your help Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From aevans3 <@t> lghealth.org Fri Nov 4 08:59:22 2011 From: aevans3 <@t> lghealth.org (Evans, Andria B) Date: Fri Nov 4 08:59:46 2011 Subject: [Histonet] Ventana Eber Probes Message-ID: <4182FDF23D7C9948BC41C4C082C3A54F03D13A7FFA3C@MAIL-AG-CLUSTER.lha.org> I am struggling with my EBER probe on both the Ventana Benchmark XT's and Ultra. I was hoping that someone could help me out with what you are currently running with the new probe setup. Thank you! A. Harris (717)544-5457 ~ FAX (if it would be easier to fax the protocol) This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From PMonfils <@t> Lifespan.org Fri Nov 4 09:16:30 2011 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Nov 4 09:16:40 2011 Subject: [Histonet] low profile vs. hi profile blades In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B8858@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B8858@PEITHA.wad.pa-ucl.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E08D61B54@LSRIEXCH1.lsmaster.lifespan.org> In my opinion it doesn't make a lot of difference which type you use, since all you actually use is the 2mm or so that extends beyond the edge of the knife holder. What is crucial is that you use the type of blade your knife holder is designed for. If you try to use high profile blades in a low profile knife holder, or vice versa, you will have major problems. Also, high profile blades are available in a heavier, less flexible form for cutting denser tissues. I don't think the low profile blades offer that option, though I am not certain since I use only the high profile format. From Bauer.Karen <@t> mayo.edu Fri Nov 4 10:16:57 2011 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Fri Nov 4 10:17:03 2011 Subject: [Histonet] Ventana Eber Probes In-Reply-To: <4182FDF23D7C9948BC41C4C082C3A54F03D13A7FFA3C@MAIL-AG-CLUSTER.lha.org> References: <4182FDF23D7C9948BC41C4C082C3A54F03D13A7FFA3C@MAIL-AG-CLUSTER.lha.org> Message-ID: <53FC421CC200C5429929EDE6C3676F3001A57EB5@msgebe34> Hi Andria, This is our EBER protocol that we are using on our XT... With great success. Using the XT ISH Open Probes - Chromogenic procedure... Depar Enzyme: ISH Protease 3 for 12 minutes Probe HybReady Probe Auto Dispense: Then pick the probe that contains EBER Denature: 60 degrees C for 4 minutes Hybridization: 37 degrees C for 1 hour Stringency Washes Stringency Wash #1: 47 degrees C for 8 minutes Stringency Wash #2: 8 minutes Stringency Wash #3: 8 minutes Detection Kit Blue Detection Since Default is iView Blue+, just put 32 minutes in for the Substrate Incubation Time Counterstain: Red Stain II for 4 minutes This protocol has worked well for us. We save our Ultra for routine IPs throughout the day and only use the XT for ISH runs, since they take longer. Good Luck, Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Pathology Department Mayo Clinic Health System in Eau Claire Phone: 715-838-3205 E-mail: bauer.karen@mayo.edu ___________________________________________ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B Sent: Friday, November 04, 2011 8:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Eber Probes I am struggling with my EBER probe on both the Ventana Benchmark XT's and Ultra. I was hoping that someone could help me out with what you are currently running with the new probe setup. Thank you! A. Harris (717)544-5457 ~ FAX (if it would be easier to fax the protocol) This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mamawooo <@t> hotmail.com Fri Nov 4 10:57:27 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Fri Nov 4 10:57:31 2011 Subject: [Histonet] Specimen Tracking for courier In-Reply-To: <001501cc9a3b$15135c00$3f3a1400$@com> References: <85760CECEC18444BB95F26D5E88DAEAA22ED9467DF@hinet2.hinet.org>, <001501cc9a3b$15135c00$3f3a1400$@com> Message-ID: Yes, Call your Ventana rep. Vantage is able to track courier pick-ups and record what slides are sent, etc. Jan Mahoney Omaha > From: cpyse@x-celllab.com > To: Jennifer.Bull@northwestpathology.com; histonet@lists.utsouthwestern.edu > Date: Thu, 3 Nov 2011 11:13:02 -0400 > Subject: RE: [Histonet] Specimen Tracking for courier > CC: > > I would be interested in everyone responses. > > Cindy > > Cindy Pyse, CLT, HT (ASCP) > Laboratory Manger > X-Cell Laboratories > e-mail cpyse@x-celllab.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bull, > Jennifer L. > Sent: Thursday, November 03, 2011 10:43 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Specimen Tracking for courier > > > > I'm curious what methods other labs are using to track specimens that > couriers pick up from clinic locations to ensure safe delivery to the lab. > We currently utilize a logbook at the clinic site that the courier signs > when they take the specimen but I have heard there are barcoded systems > available out there as well. What works? What doesn't? Appreciate your > feedback! > > Jenny > > > mailgate.hinet.org made the following annotations > --------------------------------------------------------------------- > NOTICE: This email message is for the sole use of the intended recipient(s) > and may contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not the > intended recipient, please contact the sender by reply email and destroy > all > copies of the original message. > --------------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mamawooo <@t> hotmail.com Fri Nov 4 11:05:22 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Fri Nov 4 11:05:27 2011 Subject: [Histonet] Staffing to work ratios In-Reply-To: References: Message-ID: I have always used the 5000 blocks per HT for the year. (not including IHC staff) Jan Mahoney Omaha,NE > Date: Thu, 3 Nov 2011 13:03:44 -0400 > From: Stacy_McLaughlin@cooley-dickinson.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Staffing to work ratios > > Hi, > > I know this subject has been discussed before, but I'm having trouble > finding the info I need from the archives. > > Would anyone know where I can find the work to staffing ratios for a > Histology laboratory? > > Our volume was ~9750 surgical cases (2010). The majority of them are > biopsies (GI, GYN, skin, etc) but we do some larger complex cases. > > We had 2569 billable IHC tests, 254 billable group I special stains, 722 > billable group II special stains. > > #blocks: > > 23,380 > > > > # H&E slides : 49,524 > > > > Thank you for your help! > > > > Stacy McLaughlin, HT(ASCP) > > Histology Supervisor > > Cooley Dickinson Hospital > > 30 Locust Street > > Northampton, MA 01060 > > (413)582-2019 > > Stacy_McLaughlin@Cooley-Dickinson.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mamawooo <@t> hotmail.com Fri Nov 4 11:10:01 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Fri Nov 4 11:10:10 2011 Subject: [Histonet] Training and Competency Assessment for H&E Slide Review In-Reply-To: References: Message-ID: A great way to do this is by using the results of the Histo QIP from CAP and the NSH. If you participate you get wonderful study and competency materials to use for routine H&E's special stains and IHC. Jan Mhaoney Omaha > From: joelleweaver@hotmail.com > To: Diana.Harris@viha.ca; histonet@lists.utsouthwestern.edu > Date: Thu, 3 Nov 2011 17:42:53 +0000 > Subject: Re: [Histonet] Training and Competency Assessment for H&E Slide Review > CC: > > Any certified histologist has gone through this, but the NSH has good resources for this. > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: Harris Diana > Date: Thu, 3 Nov 2011 17:34:03 > To: > Subject: [Histonet] Training and Competency Assessment for H&E Slide Review > > Any suggestions for creating a Training and Competency (T&C) Assessment for H&E slide review? We currently QC all H&E slides macroscopically and 15% microscopically. I would like to have all Histotechs trained and competent to QC H&E slides. Has anyone gone thru this process? > > Thanks > Diana Harris > QC & Method Development Technologist > Royal Jubilee Hospital > Victoria, BC Canada > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mamawooo <@t> hotmail.com Fri Nov 4 11:12:39 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Fri Nov 4 11:12:46 2011 Subject: [Histonet] Staffing to work ratios In-Reply-To: References: , Message-ID: Sorry, i meant to say 5000 cases, not blocks. Jan From: mamawooo@hotmail.com > To: stacy_mclaughlin@cooley-dickinson.org; histonet@lists.utsouthwestern.edu > Date: Fri, 4 Nov 2011 06:05:22 -1000 > Subject: RE: [Histonet] Staffing to work ratios > CC: > > > I have always used the 5000 blocks per HT for the year. (not including IHC staff) > > Jan Mahoney > Omaha,NE > > > Date: Thu, 3 Nov 2011 13:03:44 -0400 > > From: Stacy_McLaughlin@cooley-dickinson.org > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Staffing to work ratios > > > > Hi, > > > > I know this subject has been discussed before, but I'm having trouble > > finding the info I need from the archives. > > > > Would anyone know where I can find the work to staffing ratios for a > > Histology laboratory? > > > > Our volume was ~9750 surgical cases (2010). The majority of them are > > biopsies (GI, GYN, skin, etc) but we do some larger complex cases. > > > > We had 2569 billable IHC tests, 254 billable group I special stains, 722 > > billable group II special stains. > > > > #blocks: > > > > 23,380 > > > > > > > > # H&E slides : 49,524 > > > > > > > > Thank you for your help! > > > > > > > > Stacy McLaughlin, HT(ASCP) > > > > Histology Supervisor > > > > Cooley Dickinson Hospital > > > > 30 Locust Street > > > > Northampton, MA 01060 > > > > (413)582-2019 > > > > Stacy_McLaughlin@Cooley-Dickinson.org > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Fri Nov 4 11:15:36 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Nov 4 11:15:45 2011 Subject: [Histonet] Staffing to work ratios In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974D19@is-e2k3.grhs.net> That is only 20 blocks per working day (Mon-Fri)! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, November 04, 2011 11:05 AM To: stacy_mclaughlin@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios I have always used the 5000 blocks per HT for the year. (not including IHC staff) Jan Mahoney Omaha,NE > Date: Thu, 3 Nov 2011 13:03:44 -0400 > From: Stacy_McLaughlin@cooley-dickinson.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Staffing to work ratios > > Hi, > > I know this subject has been discussed before, but I'm having trouble > finding the info I need from the archives. > > Would anyone know where I can find the work to staffing ratios for a > Histology laboratory? > > Our volume was ~9750 surgical cases (2010). The majority of them are > biopsies (GI, GYN, skin, etc) but we do some larger complex cases. > > We had 2569 billable IHC tests, 254 billable group I special stains, > 722 billable group II special stains. > > #blocks: > > 23,380 > > > > # H&E slides : 49,524 > > > > Thank you for your help! > > > > Stacy McLaughlin, HT(ASCP) > > Histology Supervisor > > Cooley Dickinson Hospital > > 30 Locust Street > > Northampton, MA 01060 > > (413)582-2019 > > Stacy_McLaughlin@Cooley-Dickinson.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Fri Nov 4 11:17:21 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Nov 4 11:17:25 2011 Subject: [Histonet] Staffing to work ratios In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974D19@is-e2k3.grhs.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974D1A@is-e2k3.grhs.net> Sorry Jan, I posted before your repost. That makes more sense. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, November 04, 2011 11:16 AM To: Janice Mahoney; stacy_mclaughlin@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios That is only 20 blocks per working day (Mon-Fri)! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, November 04, 2011 11:05 AM To: stacy_mclaughlin@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios I have always used the 5000 blocks per HT for the year. (not including IHC staff) Jan Mahoney Omaha,NE > Date: Thu, 3 Nov 2011 13:03:44 -0400 > From: Stacy_McLaughlin@cooley-dickinson.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Staffing to work ratios > > Hi, > > I know this subject has been discussed before, but I'm having trouble > finding the info I need from the archives. > > Would anyone know where I can find the work to staffing ratios for a > Histology laboratory? > > Our volume was ~9750 surgical cases (2010). The majority of them are > biopsies (GI, GYN, skin, etc) but we do some larger complex cases. > > We had 2569 billable IHC tests, 254 billable group I special stains, > 722 billable group II special stains. > > #blocks: > > 23,380 > > > > # H&E slides : 49,524 > > > > Thank you for your help! > > > > Stacy McLaughlin, HT(ASCP) > > Histology Supervisor > > Cooley Dickinson Hospital > > 30 Locust Street > > Northampton, MA 01060 > > (413)582-2019 > > Stacy_McLaughlin@Cooley-Dickinson.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Nov 4 11:36:28 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Nov 4 11:37:19 2011 Subject: [Histonet] Staffing to work ratios In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974D19@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974D19@is-e2k3.grhs.net> Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E183CF8@evcspmbx3.ads.northwestern.edu> I wish! Try at least 100blk /day. And that is on an easy day. Can I work with you all? We were taught to cut a block a minute, levels or not. At slowest, one ice tray of 14 blocks in 20 minutes ,with quality. And yes ,I can still do it after all these years. When I started I had 3 months (probation) to get the quality and quantity of techs that had been around for at least 10 years and I was straight out of histo school. Well taught, I might add. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, November 04, 2011 11:16 AM To: Janice Mahoney; stacy_mclaughlin@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios That is only 20 blocks per working day (Mon-Fri)! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, November 04, 2011 11:05 AM To: stacy_mclaughlin@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios I have always used the 5000 blocks per HT for the year. (not including IHC staff) Jan Mahoney Omaha,NE > Date: Thu, 3 Nov 2011 13:03:44 -0400 > From: Stacy_McLaughlin@cooley-dickinson.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Staffing to work ratios > > Hi, > > I know this subject has been discussed before, but I'm having trouble > finding the info I need from the archives. > > Would anyone know where I can find the work to staffing ratios for a > Histology laboratory? > > Our volume was ~9750 surgical cases (2010). The majority of them are > biopsies (GI, GYN, skin, etc) but we do some larger complex cases. > > We had 2569 billable IHC tests, 254 billable group I special stains, > 722 billable group II special stains. > > #blocks: > > 23,380 > > > > # H&E slides : 49,524 > > > > Thank you for your help! > > > > Stacy McLaughlin, HT(ASCP) > > Histology Supervisor > > Cooley Dickinson Hospital > > 30 Locust Street > > Northampton, MA 01060 > > (413)582-2019 > > Stacy_McLaughlin@Cooley-Dickinson.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Nov 4 11:50:49 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Fri Nov 4 11:50:55 2011 Subject: [Histonet] Staffing to work ratios Message-ID: Sounds more accurate to expectations in my experience as well. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Bernice Frederick Date: Fri, 4 Nov 2011 16:36:28 To: ; ; ; Subject: RE: [Histonet] Staffing to work ratios I wish! Try at least 100blk /day. And that is on an easy day. Can I work with you all? We were taught to cut a block a minute, levels or not. At slowest, one ice tray of 14 blocks in 20 minutes ,with quality. And yes ,I? can still do it after all these years. When I started I had 3 months (probation) to get the quality and quantity of techs that had been around for at least 10 years and I was straight out of histo school. Well taught, I might add. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, November 04, 2011 11:16 AM To: Janice Mahoney; stacy_mclaughlin@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios That is only 20 blocks per working day (Mon-Fri)! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, November 04, 2011 11:05 AM To: stacy_mclaughlin@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios I have always used the 5000 blocks per HT for the year. (not including IHC staff) ? Jan Mahoney Omaha,NE > Date: Thu, 3 Nov 2011 13:03:44 -0400 > From: Stacy_McLaughlin@cooley-dickinson.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Staffing to work ratios > > Hi, > > I know this subject has been discussed before, but I'm having trouble > finding the info I need from the archives. > > Would anyone know where I can find the work to staffing ratios for a > Histology laboratory? > > Our volume was ~9750 surgical cases (2010). The majority of them are > biopsies (GI, GYN, skin, etc) but we do some larger complex cases. > > We had 2569 billable IHC tests, 254 billable group I special stains, > 722 billable group II special stains. > > #blocks: > > 23,380 > > > > # H&E slides : 49,524 > > > > Thank you for your help! > > > > Stacy McLaughlin, HT(ASCP) > > Histology Supervisor > > Cooley Dickinson Hospital > > 30 Locust Street > > Northampton, MA 01060 > > (413)582-2019 > > Stacy_McLaughlin@Cooley-Dickinson.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbrya <@t> lexclin.com Fri Nov 4 11:50:54 2011 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Fri Nov 4 11:51:00 2011 Subject: [Histonet] processing program for biopsies Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37519@EXCHANGESB> Would you experienced histotechs mind to look at this program and see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols. The pathologists say they look bad. Thanks in advance for your help! Station Solution Time Temp P/V Mix ------- ---------------------- ----- ----- ----- ------ 1 Formalin 0:20 OFF P/V Slow 2 Formalin 0:20 OFF P/V Slow 3 70% Alcohol 0:15 OFF P/V Slow 4 80% Alcohol 0:15 OFF P/V Slow 5 95% Alcohol 0:15 OFF P/V Slow 6 95% Alcohol 0:25 OFF P/V Slow 7 100% Alcohol 0:25 OFF P/V Slow 8 100% Alcohol 0:25 OFF P/V Slow 9 Xylene 0:35 OFF P/V Slow 10 Xylene 0:35 OFF P/V Slow 11 Paraffin 0:15 63C. P/V Slow 12 Paraffin 0:15 63C. P/V Slow 13 Paraffin 0:15 63C. P/V Slow 14 Paraffin 0:15 63C. P/V Slow Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From jclark <@t> pcnm.com Fri Nov 4 12:01:06 2011 From: jclark <@t> pcnm.com (Joanne Clark) Date: Fri Nov 4 12:01:23 2011 Subject: [Histonet] Aspyra LIS system Message-ID: <0494A7D4E8CC254EA2FB81464982E3784CA96371@S10MAILD001N2.SH10.lan> Hi Histonetters, have any of you out there had any experience with the Aspyra LIS system, specifically the anatomical pathology and cytology modules? Any feedback would be appreciated. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexcio Roswell, NM From mamawooo <@t> hotmail.com Fri Nov 4 12:13:45 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Fri Nov 4 12:13:49 2011 Subject: [Histonet] Staffing to work ratios In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF1E183CF8@evcspmbx3.ads.northwestern.edu> References: , <661949901A768E4F9CC16D8AF8F2838C03974D19@is-e2k3.grhs.net>, <62C639732D3F274DACED033EBDF6ADAF1E183CF8@evcspmbx3.ads.northwestern.edu> Message-ID: I believe the NSH published standards a few years back that suggested the average for embedding is one minute per block and 2.5 minutes for sectioning. My personal opinion is that one minute is too fast for sectioning, unless you have already faced in and are just taking a section off the top of the block. It can be done, no doubt, but for how long and at what expence? I would think that after about an hour the quality would suffer as well as the tech from repetative motion. I hope you are taking your ergonomic breaks. Jan Mahoney Omaha, NE > From: b-frederick@northwestern.edu > To: mpence@grhs.net; mamawooo@hotmail.com; stacy_mclaughlin@cooley-dickinson.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Staffing to work ratios > Date: Fri, 4 Nov 2011 16:36:28 +0000 > > I wish! Try at least 100blk /day. And that is on an easy day. Can I work with you all? We were taught to cut a block a minute, levels or not. At slowest, one ice tray of 14 blocks in 20 minutes ,with quality. And yes ,I can still do it after all these years. When I started I had 3 months (probation) to get the quality and quantity of techs that had been around for at least 10 years and I was straight out of histo school. Well taught, I might add. > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence > Sent: Friday, November 04, 2011 11:16 AM > To: Janice Mahoney; stacy_mclaughlin@cooley-dickinson.org; histo net > Subject: RE: [Histonet] Staffing to work ratios > > That is only 20 blocks per working day (Mon-Fri)! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney > Sent: Friday, November 04, 2011 11:05 AM > To: stacy_mclaughlin@cooley-dickinson.org; histo net > Subject: RE: [Histonet] Staffing to work ratios > > > > I have always used the 5000 blocks per HT for the year. (not including IHC staff) > > Jan Mahoney > Omaha,NE > > > Date: Thu, 3 Nov 2011 13:03:44 -0400 > > From: Stacy_McLaughlin@cooley-dickinson.org > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Staffing to work ratios > > > > Hi, > > > > I know this subject has been discussed before, but I'm having trouble > > finding the info I need from the archives. > > > > Would anyone know where I can find the work to staffing ratios for a > > Histology laboratory? > > > > Our volume was ~9750 surgical cases (2010). The majority of them are > > biopsies (GI, GYN, skin, etc) but we do some larger complex cases. > > > > We had 2569 billable IHC tests, 254 billable group I special stains, > > 722 billable group II special stains. > > > > #blocks: > > > > 23,380 > > > > > > > > # H&E slides : 49,524 > > > > > > > > Thank you for your help! > > > > > > > > Stacy McLaughlin, HT(ASCP) > > > > Histology Supervisor > > > > Cooley Dickinson Hospital > > > > 30 Locust Street > > > > Northampton, MA 01060 > > > > (413)582-2019 > > > > Stacy_McLaughlin@Cooley-Dickinson.org > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Nov 4 12:37:55 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Nov 4 12:38:00 2011 Subject: [Histonet] RE: processing program for biopsies In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37519@EXCHANGESB> Message-ID: <14E2C6176416974295479C64A11CB9AE011380AC6F86@SBS2K8.premierlab.local> In my personal opinion I think the processing cycle is too short. I know that skin biopsies are tiny but we process all skin samples on longer processing cycles. Granted I'm in research but we use 45 minutes to 1 hour per station for all of our skin samples and they cut just fine. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Friday, November 04, 2011 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing program for biopsies Importance: High Would you experienced histotechs mind to look at this program and see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols. The pathologists say they look bad. Thanks in advance for your help! Station Solution Time Temp P/V Mix ------- ---------------------- ----- ----- ----- ------ 1 Formalin 0:20 OFF P/V Slow 2 Formalin 0:20 OFF P/V Slow 3 70% Alcohol 0:15 OFF P/V Slow 4 80% Alcohol 0:15 OFF P/V Slow 5 95% Alcohol 0:15 OFF P/V Slow 6 95% Alcohol 0:25 OFF P/V Slow 7 100% Alcohol 0:25 OFF P/V Slow 8 100% Alcohol 0:25 OFF P/V Slow 9 Xylene 0:35 OFF P/V Slow 10 Xylene 0:35 OFF P/V Slow 11 Paraffin 0:15 63C. P/V Slow 12 Paraffin 0:15 63C. P/V Slow 13 Paraffin 0:15 63C. P/V Slow 14 Paraffin 0:15 63C. P/V Slow Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gilchrie <@t> DENTISTRY.UNC.EDU Fri Nov 4 12:42:32 2011 From: gilchrie <@t> DENTISTRY.UNC.EDU (Gilchrist, Eric) Date: Fri Nov 4 12:43:28 2011 Subject: [Histonet] RE:Contents of Histonet Digest, Vol 96, Issue 8 processing program Message-ID: <6B0498A1D99925478E639D9FAD568C2B018F1BA3F2@dentistrymail1.dentistry.unc.edu> Was this protocol working in the past? Depending on the size of the tissue specimens, I would suspect that they are Not completely dehydrated and poorly infiltrated. Eric -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, November 04, 2011 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 96, Issue 8 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Staffing to work ratios (Mike Pence) 2. RE: Staffing to work ratios (Bernice Frederick) 3. Re: Staffing to work ratios (joelle weaver ) 4. processing program for biopsies (Carol Bryant) ---------------------------------------------------------------------- Message: 1 Date: Fri, 4 Nov 2011 11:17:21 -0500 From: "Mike Pence" Subject: RE: [Histonet] Staffing to work ratios To: "Mike Pence" , "Janice Mahoney" , , "histo net" Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974D1A@is-e2k3.grhs.net> Content-Type: text/plain; charset="US-ASCII" Sorry Jan, I posted before your repost. That makes more sense. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, November 04, 2011 11:16 AM To: Janice Mahoney; stacy_mclaughlin@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios That is only 20 blocks per working day (Mon-Fri)! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, November 04, 2011 11:05 AM To: stacy_mclaughlin@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios I have always used the 5000 blocks per HT for the year. (not including IHC staff) Jan Mahoney Omaha,NE > Date: Thu, 3 Nov 2011 13:03:44 -0400 > From: Stacy_McLaughlin@cooley-dickinson.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Staffing to work ratios > > Hi, > > I know this subject has been discussed before, but I'm having trouble > finding the info I need from the archives. > > Would anyone know where I can find the work to staffing ratios for a > Histology laboratory? > > Our volume was ~9750 surgical cases (2010). The majority of them are > biopsies (GI, GYN, skin, etc) but we do some larger complex cases. > > We had 2569 billable IHC tests, 254 billable group I special stains, > 722 billable group II special stains. > > #blocks: > > 23,380 > > > > # H&E slides : 49,524 > > > > Thank you for your help! > > > > Stacy McLaughlin, HT(ASCP) > > Histology Supervisor > > Cooley Dickinson Hospital > > 30 Locust Street > > Northampton, MA 01060 > > (413)582-2019 > > Stacy_McLaughlin@Cooley-Dickinson.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Fri, 4 Nov 2011 16:36:28 +0000 From: Bernice Frederick Subject: RE: [Histonet] Staffing to work ratios To: Mike Pence , Janice Mahoney , "stacy_mclaughlin@cooley-dickinson.org" , histo net Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E183CF8@evcspmbx3.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" I wish! Try at least 100blk /day. And that is on an easy day. Can I work with you all? We were taught to cut a block a minute, levels or not. At slowest, one ice tray of 14 blocks in 20 minutes ,with quality. And yes ,I can still do it after all these years. When I started I had 3 months (probation) to get the quality and quantity of techs that had been around for at least 10 years and I was straight out of histo school. Well taught, I might add. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, November 04, 2011 11:16 AM To: Janice Mahoney; stacy_mclaughlin@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios That is only 20 blocks per working day (Mon-Fri)! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, November 04, 2011 11:05 AM To: stacy_mclaughlin@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios I have always used the 5000 blocks per HT for the year. (not including IHC staff) Jan Mahoney Omaha,NE > Date: Thu, 3 Nov 2011 13:03:44 -0400 > From: Stacy_McLaughlin@cooley-dickinson.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Staffing to work ratios > > Hi, > > I know this subject has been discussed before, but I'm having trouble > finding the info I need from the archives. > > Would anyone know where I can find the work to staffing ratios for a > Histology laboratory? > > Our volume was ~9750 surgical cases (2010). The majority of them are > biopsies (GI, GYN, skin, etc) but we do some larger complex cases. > > We had 2569 billable IHC tests, 254 billable group I special stains, > 722 billable group II special stains. > > #blocks: > > 23,380 > > > > # H&E slides : 49,524 > > > > Thank you for your help! > > > > Stacy McLaughlin, HT(ASCP) > > Histology Supervisor > > Cooley Dickinson Hospital > > 30 Locust Street > > Northampton, MA 01060 > > (413)582-2019 > > Stacy_McLaughlin@Cooley-Dickinson.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 4 Nov 2011 16:50:49 +0000 From: "joelle weaver " Subject: Re: [Histonet] Staffing to work ratios To: "Bernice Frederick " , "mpence@grhs.net " , "mamawooo@hotmail.com " , "stacy_mclaughlin@cooley-dickinson.org " , "histonet@lists.utsouthwestern.edu " Message-ID: Content-Type: text/plain; charset="iso-8859-15" Sounds more accurate to expectations in my experience as well. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Bernice Frederick Date: Fri, 4 Nov 2011 16:36:28 To: ; ; ; Subject: RE: [Histonet] Staffing to work ratios I wish! Try at least 100blk /day. And that is on an easy day. Can I work with you all? We were taught to cut a block a minute, levels or not. At slowest, one ice tray of 14 blocks in 20 minutes ,with quality. And yes ,I? can still do it after all these years. When I started I had 3 months (probation) to get the quality and quantity of techs that had been around for at least 10 years and I was straight out of histo school. Well taught, I might add. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, November 04, 2011 11:16 AM To: Janice Mahoney; stacy_mclaughlin@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios That is only 20 blocks per working day (Mon-Fri)! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, November 04, 2011 11:05 AM To: stacy_mclaughlin@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios I have always used the 5000 blocks per HT for the year. (not including IHC staff) ? Jan Mahoney Omaha,NE > Date: Thu, 3 Nov 2011 13:03:44 -0400 > From: Stacy_McLaughlin@cooley-dickinson.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Staffing to work ratios > > Hi, > > I know this subject has been discussed before, but I'm having trouble > finding the info I need from the archives. > > Would anyone know where I can find the work to staffing ratios for a > Histology laboratory? > > Our volume was ~9750 surgical cases (2010). The majority of them are > biopsies (GI, GYN, skin, etc) but we do some larger complex cases. > > We had 2569 billable IHC tests, 254 billable group I special stains, > 722 billable group II special stains. > > #blocks: > > 23,380 > > > > # H&E slides : 49,524 > > > > Thank you for your help! > > > > Stacy McLaughlin, HT(ASCP) > > Histology Supervisor > > Cooley Dickinson Hospital > > 30 Locust Street > > Northampton, MA 01060 > > (413)582-2019 > > Stacy_McLaughlin@Cooley-Dickinson.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 4 Nov 2011 12:50:54 -0400 From: Carol Bryant Subject: [Histonet] processing program for biopsies To: "histonet@lists.utsouthwestern.edu" Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37519@EXCHANGESB> Content-Type: text/plain; charset="us-ascii" Would you experienced histotechs mind to look at this program and see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols. The pathologists say they look bad. Thanks in advance for your help! Station Solution Time Temp P/V Mix ------- ---------------------- ----- ----- ----- ------ 1 Formalin 0:20 OFF P/V Slow 2 Formalin 0:20 OFF P/V Slow 3 70% Alcohol 0:15 OFF P/V Slow 4 80% Alcohol 0:15 OFF P/V Slow 5 95% Alcohol 0:15 OFF P/V Slow 6 95% Alcohol 0:25 OFF P/V Slow 7 100% Alcohol 0:25 OFF P/V Slow 8 100% Alcohol 0:25 OFF P/V Slow 9 Xylene 0:35 OFF P/V Slow 10 Xylene 0:35 OFF P/V Slow 11 Paraffin 0:15 63C. P/V Slow 12 Paraffin 0:15 63C. P/V Slow 13 Paraffin 0:15 63C. P/V Slow 14 Paraffin 0:15 63C. P/V Slow Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 8 *************************************** From lblazek <@t> digestivespecialists.com Fri Nov 4 12:48:42 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Nov 4 12:48:48 2011 Subject: [Histonet] RE: processing program for biopsies In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37519@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37519@EXCHANGESB> Message-ID: <5A2BD13465E061429D6455C8D6B40E39127401B47E@IBMB7Exchange.digestivespecialists.com> What does look bad mean? In what way do they look bad? Do they cut well? What tissue processor do you use? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Friday, November 04, 2011 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing program for biopsies Importance: High Would you experienced histotechs mind to look at this program and see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols. The pathologists say they look bad. Thanks in advance for your help! Station Solution Time Temp P/V Mix ------- ---------------------- ----- ----- ----- ------ 1 Formalin 0:20 OFF P/V Slow 2 Formalin 0:20 OFF P/V Slow 3 70% Alcohol 0:15 OFF P/V Slow 4 80% Alcohol 0:15 OFF P/V Slow 5 95% Alcohol 0:15 OFF P/V Slow 6 95% Alcohol 0:25 OFF P/V Slow 7 100% Alcohol 0:25 OFF P/V Slow 8 100% Alcohol 0:25 OFF P/V Slow 9 Xylene 0:35 OFF P/V Slow 10 Xylene 0:35 OFF P/V Slow 11 Paraffin 0:15 63C. P/V Slow 12 Paraffin 0:15 63C. P/V Slow 13 Paraffin 0:15 63C. P/V Slow 14 Paraffin 0:15 63C. P/V Slow Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LStadler <@t> cbiolabs.com Fri Nov 4 13:06:13 2011 From: LStadler <@t> cbiolabs.com (Lyn Stadler) Date: Fri Nov 4 13:06:20 2011 Subject: [Histonet] Zeiss Microm Rotary Microtome HM 325 Message-ID: <98CC14B915EBA84B9A326D45CC3C1DEC3AB8FA2A@cbiolabs05.CBiolabs.local> Curious if anyone out there has one of these microtomes? We have one that needs repair of the "Trimming Lever". The warranty has expired and I am trying to determine the most cost effective way to deal with it? Pay for the service call or purchase a service contract. Any information/suggestions/comments would be GREATLY appreciated! Lyn Stadler Histology Technician Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From Margaret.Perry <@t> sdstate.edu Fri Nov 4 14:58:06 2011 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Fri Nov 4 14:58:11 2011 Subject: [Histonet] Circo antibody Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE01DB82@SDSU-EX03.jacks.local> We are running out of our circo antibody from Iowa and did not find it listed in their bank. Are there any recommendations for a different source? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From shive003 <@t> umn.edu Fri Nov 4 15:18:25 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Nov 4 15:18:34 2011 Subject: [Histonet] Circo antibody References: <25F4FBA34BE9D142964ECC4525B82AEE01DB82@SDSU-EX03.jacks.local> Message-ID: <14524A3DED264695A6CBA11883C619A9@auxs.umn.edu> Margaret, You can purchase monoclonal PCV2 antibody through RTI (Rural Technologies, Inc.). Jan Shivers Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "Perry, Margaret" To: ; Sent: Friday, November 04, 2011 2:58 PM Subject: [Histonet] Circo antibody We are running out of our circo antibody from Iowa and did not find it listed in their bank. Are there any recommendations for a different source? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruppert.amysue <@t> marshfieldclinic.org Fri Nov 4 16:24:18 2011 From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue) Date: Fri Nov 4 16:24:38 2011 Subject: [Histonet] processing program for biopsies&In-Reply-To= Message-ID: <201111042124.pA4LOZ4R009956@mailhost3.mfldclin.edu> I agree that the time may be too short. I would suggest removing the 80% alcohol station and adding another 100%, with the time of 25 minutes. If you have a VIP tissue processor, you may also want to change the mix to "fast", with such short times in the stations, the processor does not have any time to do a pump in and pump out when set on "slow". If set on fast, you should get at least one mix per station. This is helpful because it breaks any surface tension created around the tissue pieces and will allow for better penetration of the solutions. I would also suggest to put the heat on the paraffins to 58 C. Too much exposure to heat can cause poor, hazy staining. If you have had this program set for awhile, and had good luck before, but now are having issues, than you need to look into the quality of the solutions on the processor. And make sure the processor is set up correctly..meaning the correct solution is in the correct station. It can happen that someone set up the solut ions incorrectly, since we are all only human and mistakes happen. good luck AmySue Ruppert,HT(ASCP) MB(ASCP) Marshfield Labs, Histology Lab ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From jesus.w.hdz <@t> gmail.com Fri Nov 4 16:41:20 2011 From: jesus.w.hdz <@t> gmail.com (Jesus Hernandez) Date: Fri Nov 4 16:31:13 2011 Subject: [Histonet] Formic Acid Recipe Message-ID: <5DF9068B-9218-4D6C-8FBF-3CC9B37DA511@gmail.com> Dear all, I was having trouble staining Human embryonic palatal mesenchymal cells with multi-stain solution. My PI told me to use formic acid on the samples so that it could increase the permeability of the methyl methacrylate. I am not sure if this is enough information, but the cells were loaded on hydroxyapatite scaffolds. I have also tried aniline blue and villaneuva stain. Any information on how I can make a solution of formic acid is appreciated along with maybe other stains I could try using. Each sample was grinded to about 50 microns. Thank you. Best Regards, Jesus W. Hernandez Graduate Student Department of Biomedical Engineering University of Texas at San Antonio jesus.w.hdz@gmail.com From ratliffjack <@t> hotmail.com Fri Nov 4 16:40:50 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Nov 4 16:40:55 2011 Subject: [Histonet] Formic Acid Recipe In-Reply-To: <5DF9068B-9218-4D6C-8FBF-3CC9B37DA511@gmail.com> References: <5DF9068B-9218-4D6C-8FBF-3CC9B37DA511@gmail.com> Message-ID: Hello Jesus! Try Sanderson's Rapid Bone Stain from Dorn and Hart Microedge (www.dornandhart.com). This is a metachromatic stain and should help you to penetrate the resin if heated at 60C and stained for 5-10 minutes depending upon section thickness. Rinse the slide in dH2O @ 60C and blot dry gently. Stain longer or shorter as necessary. Jack > From: jesus.w.hdz@gmail.com > Date: Fri, 4 Nov 2011 16:41:20 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Formic Acid Recipe > > Dear all, > > I was having trouble staining Human embryonic palatal mesenchymal cells with multi-stain solution. My PI told me to use formic acid on the samples so that it could increase the permeability of the methyl methacrylate. I am not sure if this is enough information, but the cells were loaded on hydroxyapatite scaffolds. I have also tried aniline blue and villaneuva stain. Any information on how I can make a solution of formic acid is appreciated along with maybe other stains I could try using. Each sample was grinded to about 50 microns. Thank you. > > Best Regards, > > Jesus W. Hernandez > Graduate Student > Department of Biomedical Engineering > University of Texas at San Antonio > jesus.w.hdz@gmail.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Fri Nov 4 17:04:35 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Nov 4 17:04:39 2011 Subject: [Histonet] RE: processing program for biopsies In-Reply-To: <14E2C6176416974295479C64A11CB9AE011380AC6F86@SBS2K8.premierlab.local> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37519@EXCHANGESB> <14E2C6176416974295479C64A11CB9AE011380AC6F86@SBS2K8.premierlab.local> Message-ID: <1320444275.37472.YahooMailNeo@web112306.mail.gq1.yahoo.com> I agree with Liz, its too short for big skins. I always call skins "chewy" because thats what they will be if not processed enough. ? In my experiance with this, skins that are small 1-2mm punch biopsies and shaves have done ok in a 15 min run for each with 30 min each for the formalins. Simular to what Carol showed here. ? Ask the Pathologist exactly what do they mean they look funny? washed out? etc because it could be the stain. Have you changed to any new reagents recently? ? Kim ________________________________ From: Elizabeth Chlipala To: 'Carol Bryant' ; "histonet@lists.utsouthwestern.edu" Sent: Friday, November 4, 2011 1:37 PM Subject: [Histonet] RE: processing program for biopsies In my personal opinion I think the processing cycle is too short.? I know that skin biopsies are tiny but we process all skin samples on longer processing cycles.? Granted I'm in research but we use 45 minutes to 1 hour per station for all of our skin samples and they cut just fine. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Friday, November 04, 2011 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing program for biopsies Importance: High Would you experienced histotechs mind to look at this program and see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols. ? The pathologists say they look bad. Thanks in advance for your help! ? Station? Solution? ? ? ? ? ? ? ? Time? Temp? P/V? ? Mix ? -------? ----------------------? -----? -----? -----? ------ ? ? ? 1? ? Formalin? ? ? ? ? ? ? ? 0:20? OFF? P/V? ? Slow ? ? ? 2? ? Formalin? ? ? ? ? ? ? ? 0:20? OFF? P/V? ? Slow ? ? ? 3? ? 70% Alcohol? ? ? ? ? ? ? 0:15? OFF? P/V? ? Slow ? ? ? 4? ? 80% Alcohol? ? ? ? ? ? ? 0:15? OFF? P/V? ? Slow ? ? ? 5? ? 95% Alcohol? ? ? ? ? ? ? 0:15? OFF? P/V? ? Slow ? ? ? 6? ? 95% Alcohol? ? ? ? ? ? ? 0:25? OFF? P/V? ? Slow ? ? ? 7? ? 100% Alcohol? ? ? ? ? ? 0:25? OFF? P/V? ? Slow ? ? ? 8? ? 100% Alcohol? ? ? ? ? ? 0:25? OFF? P/V? ? Slow ? ? ? 9? ? Xylene? ? ? ? ? ? ? ? ? 0:35? OFF? P/V? ? Slow ? ? ? 10? ? Xylene? ? ? ? ? ? ? ? ? 0:35? OFF? P/V? ? Slow ? ? ? 11? ? Paraffin? ? ? ? ? ? ? ? 0:15? 63C.? P/V? ? Slow ? ? ? 12? ? Paraffin? ? ? ? ? ? ? ? 0:15? 63C.? P/V? ? Slow ? ? ? 13? ? Paraffin? ? ? ? ? ? ? ? 0:15? 63C.? P/V? ? Slow ? ? ? 14? ? Paraffin? ? ? ? ? ? ? ? 0:15? 63C.? P/V? ? Slow Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations.? If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited.? Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Fri Nov 4 17:26:10 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Nov 4 17:26:15 2011 Subject: [Histonet] Staffing to work ratios In-Reply-To: References: , <661949901A768E4F9CC16D8AF8F2838C03974D19@is-e2k3.grhs.net>, <62C639732D3F274DACED033EBDF6ADAF1E183CF8@evcspmbx3.ads.northwestern.edu> Message-ID: <1320445570.68247.YahooMailNeo@web112317.mail.gq1.yahoo.com> I agree Jan. Ive always been leary of the one block per minute thing( and I was asked/and done?that when I first started my career). My rule has always to expect an average of 25 blocks per hour. In 4 hours a good Histotech should be able to cut 100 blocks and have time for?a break in between. Rest of the day spent doing other task. (give or take 20-30 min, some tissues are testy) ? and even to do this, you cant be all talking all over the place. it requires focus or somethings going to get messed up. ?:( ? my 2 cents ? Kim ________________________________ From: Janice Mahoney To: b-frederick@northwestern.edu; mpence@grhs.net; stacy_mclaughlin@cooley-dickinson.org; histo net Sent: Friday, November 4, 2011 1:13 PM Subject: RE: [Histonet] Staffing to work ratios I believe the NSH published standards a few years back that suggested the average for embedding is one minute per block and 2.5 minutes for sectioning.? My personal opinion is that one minute is too fast for sectioning, unless you have already faced in and are just taking a section off the top of the block. It can be done, no doubt, but for how long and at what expence?? I would think that after about an hour the quality would suffer as well as the tech from repetative motion.? I hope you are taking your ergonomic breaks. Jan Mahoney Omaha, NE > From: b-frederick@northwestern.edu > To: mpence@grhs.net; mamawooo@hotmail.com; stacy_mclaughlin@cooley-dickinson.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Staffing to work ratios > Date: Fri, 4 Nov 2011 16:36:28 +0000 > > I wish! Try at least 100blk /day. And that is on an easy day. Can I work with you all? We were taught to cut a block a minute, levels or not. At slowest, one ice tray of 14 blocks in 20 minutes ,with quality. And yes ,I can still do it after all these years. When I started I had 3 months (probation) to get the quality and quantity of techs that had been around for at least 10 years and I was straight out of histo school. Well taught, I might add. > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence > Sent: Friday, November 04, 2011 11:16 AM > To: Janice Mahoney; stacy_mclaughlin@cooley-dickinson.org; histo net > Subject: RE: [Histonet] Staffing to work ratios > > That is only 20 blocks per working day (Mon-Fri)! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney > Sent: Friday, November 04, 2011 11:05 AM > To: stacy_mclaughlin@cooley-dickinson.org; histo net > Subject: RE: [Histonet] Staffing to work ratios > > > > I have always used the 5000 blocks per HT for the year. (not including IHC staff) > > Jan Mahoney > Omaha,NE > > > Date: Thu, 3 Nov 2011 13:03:44 -0400 > > From: Stacy_McLaughlin@cooley-dickinson.org > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Staffing to work ratios > > > > Hi, > > > > I know this subject has been discussed before, but I'm having trouble > > finding the info I need from the archives. > > > > Would anyone know where I can find the work to staffing ratios for a > > Histology laboratory? > > > > Our volume was ~9750 surgical cases (2010). The majority of them are > > biopsies (GI, GYN, skin, etc) but we do some larger complex cases. > > > > We had 2569 billable IHC tests, 254 billable group I special stains, > > 722 billable group II special stains. > > > > #blocks: > > > > 23,380 > > > > > > > > # H&E slides : 49,524 > > > > > > > > Thank you for your help! > > > > > > > > Stacy McLaughlin, HT(ASCP) > > > > Histology Supervisor > > > > Cooley Dickinson Hospital > > > > 30 Locust Street > > > > Northampton, MA 01060 > > > > (413)582-2019 > > > > Stacy_McLaughlin@Cooley-Dickinson.org > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gaviotalopez_pr <@t> hotmail.com Fri Nov 4 22:28:57 2011 From: gaviotalopez_pr <@t> hotmail.com (Zoe rosa) Date: Fri Nov 4 22:29:00 2011 Subject: [Histonet] paraffin temperature Message-ID: Hello all, I need help with something basic: What is the melting temperature for paraffin? I appreciate your help, Thanks, Ismael From jmacdonald <@t> mtsac.edu Fri Nov 4 22:59:47 2011 From: jmacdonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Nov 4 22:59:57 2011 Subject: [Histonet] paraffin temperature In-Reply-To: References: Message-ID: <3966F8F4-56EC-4299-9B98-E8B11A87F510@mtsac.edu> It depends on the paraffin. The melting point is usually on the bag. Sent from my iPhone On Nov 4, 2011, at 8:28 PM, Zoe rosa wrote: > > Hello all, > > I need help with something basic: What is the melting temperature for paraffin? > > I appreciate your help, > > Thanks, Ismael _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tgenade <@t> gmail.com Sat Nov 5 04:02:57 2011 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Sat Nov 5 04:05:34 2011 Subject: [Histonet] sections lifting along the edges Message-ID: Hello, I have a problem with sections which lift off the slides. The slides are APTES coated and it isn't the entire section lifting off the slide, just the peripheral bits. I did not prepare the tissue nor section it. I am told the rat was killed and perfused with PFA for 10 minutes and then the brain removed and dehydrated in sucrose. It was NOT postfixed overnight in PFA. The dehydrated brain was then set in tissue tech and sectioned on a cryostat. 40 micron sections were made. These sections are lifting along the periphery. What is more is that antibody staining is not consistent through the sections and antibody penetration is only about 4 microns. Various conflicting reasons are given for this lifting and antibody inconsistency problem. The histologist suspects that the tissue was either not fixed enough or the the sections were not left to adhere to the APTES slide for long enough before being frozen at -20 oC. The one prof things the tissue was over-fixed. One of the antibodies we are using is the cannabinoid receptor CB1 antibody. Our signal is really poor and bleach very quickly. We found an article where the tissue was postfixed (presumably in PFA) over night and the staining is excellent... The person who did the section reported that she saw what looked like ice-crystals in the tissue. I wonder if this could be a sign that the fixation was not homogenous, i.e. the tissue now had a different texture which made it look like ice crystals. I also wonder about the -80 oC freezer which was used. It is very full and so probably serving more as an insulator than a rapid freezer. As such freezing may have been slow and ice-crystals could have formed but I don't really see tissue damage typical of freezing and the sections life along the periphery not the center... Any ideas on what is happening would be much appreciated. The person I am working with still has rats to kill and we would like to be sure that their brains will be fixed properly and not go to waste. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From one_angel_secret <@t> yahoo.com Sat Nov 5 09:43:31 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sat Nov 5 09:43:37 2011 Subject: [Histonet] sections lifting along the edges In-Reply-To: References: Message-ID: <1320504211.61990.YahooMailNeo@web112310.mail.gq1.yahoo.com> Seems to me if you can fix the sections on the slides before staining that might help. However, Ive seen when using thick sections as this?is where the periphery of the tissue meets the slide?creates a?gradient where the reagents incounter the most resistance, causing the section to peel away(also a more gentle method?)(heat?). As far as the low antibody sensativity, perhaps you could try longer incubation. I found a couple of articles on this subject that might help. Also, every article I have seen on this says to store your antibody at-20C . We always stored our antibodies at -60C so I am not sure if this is something specific to this particular antibody. Here's the articles. Hope this helps. ? http://www.ihcworld.com/smf/index.php?topic=6257.0 ? http://www.labome.com/product/Thermo-Scientific-Pierce-Products/PA1-745.html ? This next article actually has a IHC and IF method for this antibody ? http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2324193/? ? Kim ? ? ? ? ________________________________ From: Tyrone Genade To: histonet@lists.utsouthwestern.edu Sent: Saturday, November 5, 2011 5:02 AM Subject: [Histonet] sections lifting along the edges Hello, I have a problem with sections which lift off the slides. The slides are APTES coated and it isn't the entire section lifting off the slide, just the peripheral bits. I did not prepare the tissue nor section it. I am told the rat was killed and perfused with PFA for 10 minutes and then the brain removed and dehydrated in sucrose. It was NOT postfixed overnight in PFA. The dehydrated brain was then set in tissue tech and sectioned on a cryostat. 40 micron sections were made. These sections are lifting along the periphery. What is more is that antibody staining is not consistent through the sections and antibody penetration is only about 4 microns. Various conflicting reasons are given for this lifting and antibody inconsistency problem. The histologist suspects that the tissue was either not fixed enough or the the sections were not left to adhere to the APTES slide for long enough before being frozen at -20 oC. The one prof things the tissue was over-fixed. One of the antibodies we are using is the cannabinoid receptor CB1 antibody. Our signal is really poor and bleach very quickly. We found an article where the tissue was postfixed (presumably in PFA) over night and the staining is excellent... The person who did the section reported that she saw what looked like ice-crystals in the tissue. I wonder if this could be a sign that the fixation was not homogenous, i.e. the tissue now had a different texture which made it look like ice crystals. I also wonder about the -80 oC freezer which was used. It is very full and so probably serving more as an insulator than a rapid freezer. As such freezing may have been slow and ice-crystals could have formed but I don't really see tissue damage typical of freezing and the sections life along the periphery not the center... Any ideas on what is happening would be much appreciated. The person I am working with still has rats to kill and we would like to be sure that their brains will be fixed properly and not go to waste. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laura.Miller <@t> leica-microsystems.com Sat Nov 5 10:00:37 2011 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Sat Nov 5 10:01:07 2011 Subject: [Histonet] AUTO: Laura Miller is Out of the Office. (returning 11/08/2011) Message-ID: I am out of the office until 11/08/2011. I am on vacation until Tuesday. I will respond to your message when I return. Note: This is an automated response to your message "Histonet Digest, Vol 96, Issue 9" sent on 11/5/2011 9:41:14 AM. You will receive a notification for each message you send to this person while the person is away. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From cmarievernick <@t> hotmail.com Sat Nov 5 12:30:11 2011 From: cmarievernick <@t> hotmail.com (Corrie Vernick) Date: Sat Nov 5 12:30:15 2011 Subject: [Histonet] Help Message-ID: I am currently a histology student at Keiser University. I am doing a project for my routine staining class about Celestine Blue. I've been able to find information on why it was created, the chemical make up, and some of it's uses including the trichrome stain. I am having trouble finding images of slides stained with Celestine Blue. Any additional information about the uses would be helpful as well! Thank you, Corrinne VernickKeiser UniversityFL U.S.A From pruegg <@t> ihctech.net Sat Nov 5 21:24:59 2011 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sat Nov 5 21:25:10 2011 Subject: [Histonet] RE: processing program for biopsies Message-ID: <20111105192459.f86bd30e73b823f57b516b5451216a98.e7409b6284.wbe@email01.secureserver.net> i agree with Liz, the fat in the skin requires a little processing than other soft tissues especially human, animals d have as much fat and should be processed on a shorter schedule to keep them from becoming too hard and dehydrated. & -------- Subject: [Histonet] RE: processing program fo From: Elizabeth Chlipala <[1]liz@premierlab.com> Date: Fri, November 04, 2011 10:37 amTo: 'Carol Bryant' <[2]cbrya@lexclin "[3]hist <[4]histonet@lists.utsouthwestern.edu> In my per know that skin b longer processing cycle minutes to 1 hour per station for cut just fine. Liz Elizabeth Manager Premier Laboratory, LLC PO Boulder, CO 80308-1592 (303) 682-3949 office (303) 682- (303) 881-0763 cell [5]ww Ship to address: 1567 Skyway Drive, Unit Longmont, CO 80504 -----Original Message----- From: [6]histonet-bounces@list [[7]mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Carol Bryant Sent: Friday, November 04, 2011 10:51 AM To: [8]histonet@lists.utsouthwestern Subject: [Histonet] processing program for biopsies Importan Would you experienced histotechs mind to look at this progr see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols. The pathologists say they look bad. Thanks Station Solution Time Temp P/V Mix --- 1 Formalin 0:20 OFF 2 Formalin 0:20 OFF P/V Slow 3 70% Alcohol 0:15 OFF P/V Slo 4 80% Alcohol 0:15 OFF P/V Slow 5 95% Alcohol 0:15 OFF P/V Slow 7 100% Alcohol 0:25 OFF P/V Slow 8 10 9 Xylene 0:35 OFF P/V Slow 10 Xylene 0:3 11 Paraffin 0:15 63C. P/V Slow 12 Paraffin 0:15 63C. P 13 Paraffin 0:15 63C. P/V Slow 14 Paraffin 0:15 63C. P/V Slow Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexing Phone (859) 258-4082 Fax (859) 258-4081 [9]cbrya@lexclin.com NOTICE OF CONFID This message, including any attachments, is intended only sole use of the addressee and may contain confidential or privilege information that is protected by the State of Kentucky and/or Federal reg copy, retain have received this me immediately at (859)258-4000 and del and any attachment. Any unauthorized review, or distribution is strictly prohibited. Neither t this message or any attachment, nor any error in transmi misdelivery shall constitute waiver of any applicable legal privil ege. _______________________________________________ Histonet mailing [10]Histonet@list [11]http://lists.utsouthwestern.edu/mailman/listinfo/his _______________________________________________ Histone [12]Hist [13]http://lists.utsouthwestern.edu/mailman/lis References 1. 3D"mailto:liz@premierlab 2. 3D"mailto:cbrya@lexclin.com" 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"mailto:histonet@lists.uts 5. 3D"http://www.premierlab.com"/ 6. ="mailto:histonet-bounces@lists.utsouthwestern.edu" 7. 3D"mailto:histonet-bounces@lists.utsouthw 8. ="mailto:histonet@lists.utsouthwestern.edu" 9. file://localhost/tmp/3D"mail 10. 3D"mailto:Histonet@lists.utsouthwestern.edu" 11. 3D"http://lists.utsouthwestern.edu/mail 12. 3D"mailto:Histonet@lists.utsouthwestern.edu" 13. 3D"http://lists.utsouthwestern=/ From pruegg <@t> ihctech.net Sat Nov 5 21:35:57 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Nov 5 21:36:05 2011 Subject: [Histonet] sections lifting along the edges In-Reply-To: References: Message-ID: I would fix the profused fixed tissue in formalin for 12-24 hours before infiltrating it in 30% sucrose then snap freeze it, the researcher who thinks it is over fixed is wrong and that is very common, researchers think that aldehyde fixations inhibitis IHc staining but things have changed and especially with antigen retrieval techniques it is better to fix and then infiltrate in sucrose and snap freeze to get the best results, profuse fixation for 10 min is not enough. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade Sent: Saturday, November 05, 2011 3:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sections lifting along the edges Hello, I have a problem with sections which lift off the slides. The slides are APTES coated and it isn't the entire section lifting off the slide, just the peripheral bits. I did not prepare the tissue nor section it. I am told the rat was killed and perfused with PFA for 10 minutes and then the brain removed and dehydrated in sucrose. It was NOT postfixed overnight in PFA. The dehydrated brain was then set in tissue tech and sectioned on a cryostat. 40 micron sections were made. These sections are lifting along the periphery. What is more is that antibody staining is not consistent through the sections and antibody penetration is only about 4 microns. Various conflicting reasons are given for this lifting and antibody inconsistency problem. The histologist suspects that the tissue was either not fixed enough or the the sections were not left to adhere to the APTES slide for long enough before being frozen at -20 oC. The one prof things the tissue was over-fixed. One of the antibodies we are using is the cannabinoid receptor CB1 antibody. Our signal is really poor and bleach very quickly. We found an article where the tissue was postfixed (presumably in PFA) over night and the staining is excellent... The person who did the section reported that she saw what looked like ice-crystals in the tissue. I wonder if this could be a sign that the fixation was not homogenous, i.e. the tissue now had a different texture which made it look like ice crystals. I also wonder about the -80 oC freezer which was used. It is very full and so probably serving more as an insulator than a rapid freezer. As such freezing may have been slow and ice-crystals could have formed but I don't really see tissue damage typical of freezing and the sections life along the periphery not the center... Any ideas on what is happening would be much appreciated. The person I am working with still has rats to kill and we would like to be sure that their brains will be fixed properly and not go to waste. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) **************************************************************************** **** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Nov 6 13:41:56 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Nov 6 13:42:03 2011 Subject: [Histonet] Formic Acid Recipe In-Reply-To: <5DF9068B-9218-4D6C-8FBF-3CC9B37DA511@gmail.com> References: <5DF9068B-9218-4D6C-8FBF-3CC9B37DA511@gmail.com> Message-ID: <7C1A56AD0BA141F186C32E903B267A18@prueggihctechlt> Jesus, The formic acid (5%) will decalcify the hydroxyapatite which can be very hard and crunchy to cut but since you ground the section I guess you don't care about that. I would be concerned though that the formic acid might also cause you to lose staining of some cell components that depend on the calcium. The methyl methacrylate can be removed from the section by xylene like deparaffinizing paraffin sections. It works for methyl methacrylate but nothing removes glycol methacrylate. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Hernandez Sent: Friday, November 04, 2011 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formic Acid Recipe Dear all, I was having trouble staining Human embryonic palatal mesenchymal cells with multi-stain solution. My PI told me to use formic acid on the samples so that it could increase the permeability of the methyl methacrylate. I am not sure if this is enough information, but the cells were loaded on hydroxyapatite scaffolds. I have also tried aniline blue and villaneuva stain. Any information on how I can make a solution of formic acid is appreciated along with maybe other stains I could try using. Each sample was grinded to about 50 microns. Thank you. Best Regards, Jesus W. Hernandez Graduate Student Department of Biomedical Engineering University of Texas at San Antonio jesus.w.hdz@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Nov 6 13:53:29 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Nov 6 13:53:35 2011 Subject: [Histonet] low profile vs. hi profile blades In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B8858@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B8858@PEITHA.wad.pa-ucl.com> Message-ID: It depends on the microtome being used and how the blade holder is set up to take high profile or low, many of them (at least the microms I have can take either) on my microm to use low profile I leave the bar on the knife holder so the holder is set to hold shorter low profile blades, I can take that bar out with a couple of little screws on each side which deepens the knife holder space so it can take high profile knives. One of my cryostats knife holder only takes high profile blades so I like to use Sturkey gold high profile there, the other cryostat I have only takes low profile blades. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, November 04, 2011 7:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] low profile vs. hi profile blades Hi- Please educate me on this. There seems to be a lot of personal opinion involved in regards to which type of blade to use. Thanks for your help Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Nov 6 13:58:01 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Nov 6 13:58:07 2011 Subject: [Histonet] low profile vs. hi profile blades In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E08D61B54@LSRIEXCH1.lsmaster.lifespan.org> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B8858@PEITHA.wad.pa-ucl.com> <4EBFF65383B74D49995298C4976D1D5E08D61B54@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <7453DEFC823949839EB558AA26F44754@prueggihctechlt> In my experience even with the harder high profile blades for denser tissues such as bone I still prefer to use permanent tungsten carbide blades I have to send out to get sharpened. The high profile disposable blades seem to vibrate too much for me even the heavier tungsten ones for bone. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, November 04, 2011 8:17 AM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] low profile vs. hi profile blades In my opinion it doesn't make a lot of difference which type you use, since all you actually use is the 2mm or so that extends beyond the edge of the knife holder. What is crucial is that you use the type of blade your knife holder is designed for. If you try to use high profile blades in a low profile knife holder, or vice versa, you will have major problems. Also, high profile blades are available in a heavier, less flexible form for cutting denser tissues. I don't think the low profile blades offer that option, though I am not certain since I use only the high profile format. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Nov 6 14:17:01 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Nov 6 14:17:08 2011 Subject: [Histonet] Training and Competency Assessment for H&E Slide Review In-Reply-To: References: Message-ID: <852DC7E19ED446CB81356EA0BEB5F22B@prueggihctechlt> This was what I was going to suggest, I bet they let you participate in HistoQip even if you are from Canada. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, November 04, 2011 10:10 AM To: joelleweaver@hotmail.com; diana.harris@viha.ca; histo net Subject: RE: [Histonet] Training and Competency Assessment for H&E Slide Review A great way to do this is by using the results of the Histo QIP from CAP and the NSH. If you participate you get wonderful study and competency materials to use for routine H&E's special stains and IHC. Jan Mhaoney Omaha > From: joelleweaver@hotmail.com > To: Diana.Harris@viha.ca; histonet@lists.utsouthwestern.edu > Date: Thu, 3 Nov 2011 17:42:53 +0000 > Subject: Re: [Histonet] Training and Competency Assessment for H&E Slide Review > CC: > > Any certified histologist has gone through this, but the NSH has good resources for this. > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: Harris Diana > Date: Thu, 3 Nov 2011 17:34:03 > To: > Subject: [Histonet] Training and Competency Assessment for H&E Slide Review > > Any suggestions for creating a Training and Competency (T&C) Assessment for H&E slide review? We currently QC all H&E slides macroscopically and 15% microscopically. I would like to have all Histotechs trained and competent to QC H&E slides. Has anyone gone thru this process? > > Thanks > Diana Harris > QC & Method Development Technologist > Royal Jubilee Hospital > Victoria, BC Canada > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sun Nov 6 14:42:02 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sun Nov 6 14:42:07 2011 Subject: [Histonet] Re: Celestin blue B (was Help) Message-ID: Corrie Vernick writes: >>I am currently a histology student at Keiser University. I am doing a project for my routine staining class about Celestine Blue. I've been able to find information on why it was created, the chemical make up, and some of it's uses including the trichrome stain. I am having trouble finding images of slides stained with Celestine Blue. Any additional information about the uses would be helpful as well! Thank you, Corrinne Vernick, Keiser University FL U.S.A.<< I don't have access to my library this week, but you can get a good bit information by Googling celestin blue B. This dye was often used as a sort of backup or substitute for hematoxylin, particularly in the old outmoded Pearse stain for pituitary cells. R.D. Lillie as I remember didn't think much of the dye, and I don't think this dye is a very good topic for a study such as the one you describe. Bob Richmond Samurai Pathologist Knoxville TN From AnthonyH <@t> chw.edu.au Sun Nov 6 16:11:01 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Nov 6 16:11:21 2011 Subject: [Histonet] RE: processing program for biopsies In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37519@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF141CC37519@EXCHANGESB> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A027F7@xmdb02.nch.kids> Only 40 minutes in formalin is a problem. Increase the formalin time to several hours and this should suffice for small 2-3mm punch biopsies of skin. For larger skin sections the alcohol, xylene and wax times will need to be tripled at least (as well as fixing the skins for longer (3-4 hours on the machine would be great)). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Saturday, 5 November 2011 3:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing program for biopsies Importance: High Would you experienced histotechs mind to look at this program and see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols. The pathologists say they look bad. Thanks in advance for your help! Station Solution Time Temp P/V Mix ------- ---------------------- ----- ----- ----- ------ 1 Formalin 0:20 OFF P/V Slow 2 Formalin 0:20 OFF P/V Slow 3 70% Alcohol 0:15 OFF P/V Slow 4 80% Alcohol 0:15 OFF P/V Slow 5 95% Alcohol 0:15 OFF P/V Slow 6 95% Alcohol 0:25 OFF P/V Slow 7 100% Alcohol 0:25 OFF P/V Slow 8 100% Alcohol 0:25 OFF P/V Slow 9 Xylene 0:35 OFF P/V Slow 10 Xylene 0:35 OFF P/V Slow 11 Paraffin 0:15 63C. P/V Slow 12 Paraffin 0:15 63C. P/V Slow 13 Paraffin 0:15 63C. P/V Slow 14 Paraffin 0:15 63C. P/V Slow Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From lpwenk <@t> sbcglobal.net Sun Nov 6 16:36:21 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Nov 6 16:36:24 2011 Subject: [Histonet] Re: Celestin blue B (was Help) In-Reply-To: References: Message-ID: <23E40AFCC7CD4F5588F3A328052116D2@HP2010> With the hematoxylin shortage of a couple of years ago (real, not imagined in about 2007-2008), several companies tried to come up with a synthetic dye substitute. A little background: Celestine blue (CI 51050, also known as Mordant blue 14) is a substitute touted many years ago (late 1960s/early 1970s if I remember, when there was another shortage for different reasons). I've used it years ago, in a stain called MSB for fibrin (look in a Bancroft book). The MSB stain used a double nuclear stain of aluminum hematoxylin (e.g. Mayer or Harris) and celestine blue. We didn't like the celestine blue, because when we mixed the iron mordant salt with the celestine blue dye, it worked right away, but, the next time someone asked for a MSB stain (several months later), the celestine blue was overoxidized, and wouldn't work. So we would have to stop and make the dye up immediately. It was a nuisance. But the double nuclear stain to to try to keep the nuclei a blue color, after going through the next 3 dyes (MSB is sort of a quatrachrome). Eventually, we just started using the Weigert hematoxylin from the trichrome stain in place of the double nuclear stain in the MSB. Our pathologists like the MSB stain better with the Weigert hematoxylin. Current use: Since 2008, three companies that I know of came up with hematoxylin substitutes, in response to that shortage. - Anatech Ltd, which used Mordant blue 3, CI 43820, and called it Tango Blue. So this is NOT celestine blue. http://www.anatechltdusa.com/MSDS_pdf/TangoStainMSDS.pdf - Newcomer Supply, which calls their substitute Newly Blue, but says it is a Celestine blue in one solution, ferric ammonium sulfate in the other solution. I don't know if you mix the two solutions before use, or if you dip the slides in first one solution and then the other. I didn't get around to their booth this year at NSH (sorry Marcia), and have never seen a procedure sheet on this stain. http://www.newcomersupply.com/products/standard-special-stains?page=N#181 - ThermoFisher/Richard Allan, which calls their substitute Phoenix blue.The problem is, they are being secretive (proprietary) about what dye/reagents are in their solutions. I looked at one time, and couldn't find MSDS on their websites And their flyer doesn't mention the dyes name or CI #. However, the photo on the flyer, at least to me, looks like celestine blue. That's not proof that it IS celestine blue. Could be a close look-alike cousin. http://www.thermo.com/eThermo/CMA/PDFs/Product/productPDF_6906.pdf So look to these companies for more information. Anatech has a newsletter, available on their website, about the hematoxylin shortage (and their Tango blue product, of course). (Not that this will help you with your Celestine blue project, but it will fill you in on the hematoxylin shortage history.) http://www.anatechltdusa.com/Innovators/Inn08Summer.pdf Hope that helps some. Peggy A. Wenk, HTL(ASCP)SLS Schools of Histotechnology Beaumont Hospital Royal Oak, MI 48073 (Opinions expressed are my own, and not Beaumont Hospitals'.) -----Original Message----- From: Bob Richmond Sent: Sunday, November 06, 2011 3:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Celestin blue B (was Help) Corrie Vernick writes: >>I am currently a histology student at Keiser University. I am doing a >>project for my routine staining class about Celestine Blue. I've been able >>to find information on why it was created, the chemical make up, and some >>of it's uses including the trichrome stain. I am having trouble finding >>images of slides stained with Celestine Blue. Any additional information >>about the uses would be helpful as well! Thank you, Corrinne Vernick, >>Keiser University FL U.S.A.<< I don't have access to my library this week, but you can get a good bit information by Googling celestin blue B. This dye was often used as a sort of backup or substitute for hematoxylin, particularly in the old outmoded Pearse stain for pituitary cells. R.D. Lillie as I remember didn't think much of the dye, and I don't think this dye is a very good topic for a study such as the one you describe. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From piojito91583 <@t> yahoo.com Sun Nov 6 18:20:14 2011 From: piojito91583 <@t> yahoo.com (Jamie Gomez) Date: Sun Nov 6 18:20:18 2011 Subject: [Histonet] Microwave processing Message-ID: <1320625214.81706.YahooMailNeo@web114402.mail.gq1.yahoo.com> Hello, I am a histology technology student and I need information on the melting point of the paraffin used in Microwave Processing? Freida Carson stated in her book something regarding the paraffin temperature set at 84 degrees. The class had a discussion regarding the same and concluded that the tissue morphology would be damage at such high temperature. Can someone please provide me with some input on this issue. ? Thank you, Jamie G. From gaviotalopez_pr <@t> hotmail.com Sun Nov 6 20:40:35 2011 From: gaviotalopez_pr <@t> hotmail.com (Zoe rosa) Date: Sun Nov 6 20:40:40 2011 Subject: [Histonet] microwave processing Message-ID: Hi there, On an Instrumentation course at keiser, we had a discussion about the right temperature fro the paraffin when the proceesing is done using microwaves. Can some tell me what should be the ideal temperature with out damaging the processing. The text book mentions 84 degrees, but isn't this to high; perhaps if the time is less, it wont affect it? Thanks, Ismael Lopez From AnthonyH <@t> chw.edu.au Sun Nov 6 20:44:46 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood (SCHN)) Date: Sun Nov 6 20:44:54 2011 Subject: [Histonet] microwave processing In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715760A029FC@xmdb02.nch.kids> I believe that the higher temperature is needed to evaporate off the isopropanol. Just make sure that your tissue is well fixed. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zoe rosa Sent: Monday, 7 November 2011 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processing Hi there, On an Instrumentation course at keiser, we had a discussion about the right temperature fro the paraffin when the proceesing is done using microwaves. Can some tell me what should be the ideal temperature with out damaging the processing. The text book mentions 84 degrees, but isn't this to high; perhaps if the time is less, it wont affect it? Thanks, Ismael Lopez _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Tony_Reilly <@t> health.qld.gov.au Sun Nov 6 23:35:12 2011 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Sun Nov 6 23:37:49 2011 Subject: [Histonet] Re: Celestin blue B (was Help) In-Reply-To: <23E40AFCC7CD4F5588F3A328052116D2@HP2010> References: <23E40AFCC7CD4F5588F3A328052116D2@HP2010> Message-ID: <4EB7FAB0.411C.0039.0@health.qld.gov.au> Hello I have used Celestin Blue-Haemalum (Mayers) considerably in the past as a substitute for Weigert's and as you say the Celestin Blue stain does not keep and is best made fresh for each use. Our method to simplify this was to make separate double strength solutions of the Celestin blue and the mordant which were mixed 50/50 just prior to use to make a solution of the correct strength. Both of these solutions store well and the preparation time is minimal. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Lee & Peggy Wenk" 11/7/2011 8:36 am >>> With the hematoxylin shortage of a couple of years ago (real, not imagined in about 2007-2008), several companies tried to come up with a synthetic dye substitute. A little background: Celestine blue (CI 51050, also known as Mordant blue 14) is a substitute touted many years ago (late 1960s/early 1970s if I remember, when there was another shortage for different reasons). I've used it years ago, in a stain called MSB for fibrin (look in a Bancroft book). The MSB stain used a double nuclear stain of aluminum hematoxylin (e.g. Mayer or Harris) and celestine blue. We didn't like the celestine blue, because when we mixed the iron mordant salt with the celestine blue dye, it worked right away, but, the next time someone asked for a MSB stain (several months later), the celestine blue was overoxidized, and wouldn't work. So we would have to stop and make the dye up immediately. It was a nuisance. But the double nuclear stain to to try to keep the nuclei a blue color, after going through the next 3 dyes (MSB is sort of a quatrachrome). Eventually, we just started using the Weigert hematoxylin from the trichrome stain in place of the double nuclear stain in the MSB. Our pathologists like the MSB stain better with the Weigert hematoxylin. Current use: Since 2008, three companies that I know of came up with hematoxylin substitutes, in response to that shortage. - Anatech Ltd, which used Mordant blue 3, CI 43820, and called it Tango Blue. So this is NOT celestine blue. http://www.anatechltdusa.com/MSDS_pdf/TangoStainMSDS.pdf - Newcomer Supply, which calls their substitute Newly Blue, but says it is a Celestine blue in one solution, ferric ammonium sulfate in the other solution. I don't know if you mix the two solutions before use, or if you dip the slides in first one solution and then the other. I didn't get around to their booth this year at NSH (sorry Marcia), and have never seen a procedure sheet on this stain. http://www.newcomersupply.com/products/standard-special-stains?page=N#181 - ThermoFisher/Richard Allan, which calls their substitute Phoenix blue.The problem is, they are being secretive (proprietary) about what dye/reagents are in their solutions. I looked at one time, and couldn't find MSDS on their websites And their flyer doesn't mention the dyes name or CI #. However, the photo on the flyer, at least to me, looks like celestine blue. That's not proof that it IS celestine blue. Could be a close look-alike cousin. http://www.thermo.com/eThermo/CMA/PDFs/Product/productPDF_6906.pdf So look to these companies for more information. Anatech has a newsletter, available on their website, about the hematoxylin shortage (and their Tango blue product, of course). (Not that this will help you with your Celestine blue project, but it will fill you in on the hematoxylin shortage history.) http://www.anatechltdusa.com/Innovators/Inn08Summer.pdf Hope that helps some. Peggy A. Wenk, HTL(ASCP)SLS Schools of Histotechnology Beaumont Hospital Royal Oak, MI 48073 (Opinions expressed are my own, and not Beaumont Hospitals'.) -----Original Message----- From: Bob Richmond Sent: Sunday, November 06, 2011 3:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Celestin blue B (was Help) Corrie Vernick writes: >>I am currently a histology student at Keiser University. I am doing a >>project for my routine staining class about Celestine Blue. I've been able >>to find information on why it was created, the chemical make up, and some >>of it's uses including the trichrome stain. I am having trouble finding >>images of slides stained with Celestine Blue. Any additional information >>about the uses would be helpful as well! Thank you, Corrinne Vernick, >>Keiser University FL U.S.A.<< I don't have access to my library this week, but you can get a good bit information by Googling celestin blue B. This dye was often used as a sort of backup or substitute for hematoxylin, particularly in the old outmoded Pearse stain for pituitary cells. R.D. Lillie as I remember didn't think much of the dye, and I don't think this dye is a very good topic for a study such as the one you describe. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From Carmen.M.Garcia <@t> uv.es Mon Nov 7 02:48:01 2011 From: Carmen.M.Garcia <@t> uv.es (Carmen Maria Garcia Pascual) Date: Mon Nov 7 02:48:11 2011 Subject: [Histonet] Immune cells antibody In-Reply-To: <3b0b37ec1a928aab52f3d9081609fa57@ufl.edu> References: <3b0b37ec1a928aab52f3d9081609fa57@ufl.edu> Message-ID: <2427631794carmaga6@uv.es> Hi everyone: I want to detect immune cells (natural killers) in mouse's decidua, I wonder if somebody knows a good antibody for that. thanks!!! Carmen From tgenade <@t> gmail.com Mon Nov 7 04:58:20 2011 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Mon Nov 7 04:58:28 2011 Subject: [Histonet] Re: sections lifting along the edges Message-ID: Hello, I would like to thank everyone who has replied to my post. Your comments and advice are most helpful. Kind regards -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From jesus.w.hdz <@t> gmail.com Mon Nov 7 08:13:51 2011 From: jesus.w.hdz <@t> gmail.com (Jesus Hernandez) Date: Mon Nov 7 08:03:49 2011 Subject: [Histonet] Fwd: Formic Acid Recipe References: <5DF9068B-9218-4D6C-8FBF-3CC9B37DA511@gmail.com> Message-ID: Begin forwarded message: > From: Jesus Hernandez > Date: November 4, 2011 4:41:20 PM CDT > To: histonet@lists.utsouthwestern.edu > Subject: Formic Acid Recipe > > Dear all, > > I was having trouble staining Human embryonic palatal mesenchymal cells with multi-stain solution. My PI told me to use formic acid on the samples so that it could increase the permeability of the methyl methacrylate. I am not sure if this is enough information, but the cells were loaded on hydroxyapatite scaffolds. I have also tried aniline blue and villaneuva stain. Any information on how I can make a solution of formic acid is appreciated along with maybe other stains I could try using. Each sample was grinded to about 50 microns. Thank you. > > Best Regards, > > Jesus W. Hernandez > Graduate Student > Department of Biomedical Engineering > University of Texas at San Antonio > jesus.w.hdz@gmail.com > > > From amber.mckenzie <@t> gastrodocs.net Mon Nov 7 08:27:45 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Nov 7 08:26:53 2011 Subject: [Histonet] Training and Competency Assessment for H&E Slide Review In-Reply-To: <852DC7E19ED446CB81356EA0BEB5F22B@prueggihctechlt> References: <852DC7E19ED446CB81356EA0BEB5F22B@prueggihctechlt> Message-ID: <5A33C952BB67F4468AF1F36D739212BC0619C3@JERRY.Gia.com> Can I do HistoQip if I am CLIA certified or does it have to be a CAP lab? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Sunday, November 06, 2011 2:17 PM To: 'Janice Mahoney'; joelleweaver@hotmail.com; diana.harris@viha.ca; 'histo net' Subject: RE: [Histonet] Training and Competency Assessment for H&E Slide Review This was what I was going to suggest, I bet they let you participate in HistoQip even if you are from Canada. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, November 04, 2011 10:10 AM To: joelleweaver@hotmail.com; diana.harris@viha.ca; histo net Subject: RE: [Histonet] Training and Competency Assessment for H&E Slide Review A great way to do this is by using the results of the Histo QIP from CAP and the NSH. If you participate you get wonderful study and competency materials to use for routine H&E's special stains and IHC. Jan Mhaoney Omaha > From: joelleweaver@hotmail.com > To: Diana.Harris@viha.ca; histonet@lists.utsouthwestern.edu > Date: Thu, 3 Nov 2011 17:42:53 +0000 > Subject: Re: [Histonet] Training and Competency Assessment for H&E Slide Review > CC: > > Any certified histologist has gone through this, but the NSH has good resources for this. > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: Harris Diana > Date: Thu, 3 Nov 2011 17:34:03 > To: > Subject: [Histonet] Training and Competency Assessment for H&E Slide Review > > Any suggestions for creating a Training and Competency (T&C) Assessment for H&E slide review? We currently QC all H&E slides macroscopically and 15% microscopically. I would like to have all Histotechs trained and competent to QC H&E slides. Has anyone gone thru this process? > > Thanks > Diana Harris > QC & Method Development Technologist > Royal Jubilee Hospital > Victoria, BC Canada > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Mon Nov 7 08:33:28 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Nov 7 08:32:34 2011 Subject: [Histonet] paraffin temperature In-Reply-To: <3966F8F4-56EC-4299-9B98-E8B11A87F510@mtsac.edu> References: <3966F8F4-56EC-4299-9B98-E8B11A87F510@mtsac.edu> Message-ID: <5A33C952BB67F4468AF1F36D739212BC061A18@JERRY.Gia.com> I have my paraffin chambers set at 60 degrees (embedding center and processor) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, November 04, 2011 11:00 PM To: Zoe rosa Cc: Subject: Re: [Histonet] paraffin temperature It depends on the paraffin. The melting point is usually on the bag. Sent from my iPhone On Nov 4, 2011, at 8:28 PM, Zoe rosa wrote: > > Hello all, > > I need help with something basic: What is the melting temperature for paraffin? > > I appreciate your help, > > Thanks, Ismael _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Mon Nov 7 09:28:23 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Nov 7 09:28:37 2011 Subject: [Histonet] Re: Celestin blue B (was Help) In-Reply-To: References: Message-ID: I believe Celestine Blue was used much more frequently in the UK in the 70s and 80s, preferred to an iron hematoxylin, in many "trichrome" stains including Lendrum's MSB, as Peggy mentions; the nuclear staining withstanding further differentiation during the subsequent staining from either picric or phosphotungstic/phosphomolybdic acids. It is a difficult stain to prepare as it has to be boiled with glycerin in it and requires glass beads being added to prevent it foaming up and going all over the bench (don't have the exact recipe to hand, but do remember having to frequently clean up the mess during my training!) We used it extensively, and I do not remember it having a particularly short shelf-life - but it did have to be filtered regularly. Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Sunday, November 06, 2011 3:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Celestin blue B (was Help) Corrie Vernick writes: >>I am currently a histology student at Keiser University. I am doing a >>project for my routine staining class about Celestine Blue. I've been >>able to find information on why it was created, the chemical make up, >>and some of it's uses including the trichrome stain. I am having >>trouble finding images of slides stained with Celestine Blue. Any >>additional information about the uses would be helpful as well! Thank >>you, Corrinne Vernick, Keiser University FL U.S.A.<< I don't have access to my library this week, but you can get a good bit information by Googling celestin blue B. This dye was often used as a sort of backup or substitute for hematoxylin, particularly in the old outmoded Pearse stain for pituitary cells. R.D. Lillie as I remember didn't think much of the dye, and I don't think this dye is a very good topic for a study such as the one you describe. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From jkiernan <@t> uwo.ca Mon Nov 7 11:55:06 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Nov 7 11:55:20 2011 Subject: [Histonet] Re: Celestin blue B (was Help) In-Reply-To: <23E40AFCC7CD4F5588F3A328052116D2@HP2010> References: <23E40AFCC7CD4F5588F3A328052116D2@HP2010> Message-ID: <7660f03e7583a.4eb7d52a@uwo.ca> Thanks Peggy for the helpful information about recent commercial haemalum substitutes. The MSDS for tango describes only an acidified solution of the dye (eriochrome cyanine R). This would need to be mixed with a ferric salt to make a blue nuclear stain. Iron-ECR is probably the best substitute for haemalum. See Dapson R, Horobin RW & Kiernan J (2010) Hematoxylin shortages: their causes and duration, and other dyes that can replace hemalum in routine hematoxylin and eosin staining. Biotechnic & Histochemistry 85(1): 55-63. I agree with Peggy that newly blue and phoenix blue are probably iron-celestine blue, following the method of Lillie (Lillie RD, Pizzolato P, Welsh RA, Holmquist MD, Donaldson PT, Berger C (1973) A consideration of substitutes for alum hematoxylin in routine histologic and cytologic diagnostic procedures. Am. J. Clin. Path. 60: 817-819.), which uses two fairly stable stock solutions (iron alum and the dye) to make a working stain that keeps for a few weeks. Older formulations (Lendrum 1935, Gray 1956) contain the ferric salt, dye and sulphuric acid. They are more acidic (pH 0.8 to 1.1), keep for 6 months, and are also more selective for nuclei. Lendrum's stain is the one often followed by haemalum to give a black nuclear stain that resists acids (picric, phosphotungstic etc) even better than Weigert's iron-haematoxylin. If a company won't say what their product is, that's a good reason not to buy it. There's nothing new about celestine blue. John Kiernan Dept of Anatomy & Cell Biology University of Western Ontario London, Canada = = = On 06/11/11, Lee & Peggy Wenk wrote: > > With the hematoxylin shortage of a couple of years ago (real, not imagined in about 2007-2008), several companies tried to come up with a synthetic dye substitute. > > A little background: Celestine blue (CI 51050, also known as Mordant blue 14) is a substitute touted many years ago (late 1960s/early 1970s if I remember, when there was another shortage for different reasons). I've used it years ago, in a stain called MSB for fibrin (look in a Bancroft book). The MSB stain used a double nuclear stain of aluminum hematoxylin (e.g. Mayer or Harris) and celestine blue. We didn't like the celestine blue, because when we mixed the iron mordant salt with the celestine blue dye, it worked right away, but, the next time someone asked for a MSB stain (several months later), the celestine blue was overoxidized, and wouldn't work. So we would have to stop and make the dye up immediately. It was a nuisance. But the double nuclear stain to to try to keep the nuclei a blue color, after going through the next 3 dyes (MSB is sort of a quatrachrome). Eventually, we just started using the Weigert hematoxylin from the trichrome stain in place of the double nuclear stain in the MSB. Our pathologists like the MSB stain better with the Weigert hematoxylin. > > Current use: Since 2008, three companies that I know of came up with hematoxylin substitutes, in response to that shortage. > - Anatech Ltd, which used Mordant blue 3, CI 43820, and called it Tango Blue. So this is NOT celestine blue. > http://www.anatechltdusa.com/MSDS_pdf/TangoStainMSDS.pdf > - Newcomer Supply, which calls their substitute Newly Blue, but says it is a Celestine blue in one solution, ferric ammonium sulfate in the other solution. I don't know if you mix the two solutions before use, or if you dip the slides in first one solution and then the other. I didn't get around to their booth this year at NSH (sorry Marcia), and have never seen a procedure sheet on this stain. > http://www.newcomersupply.com/products/standard-special-stains?page=N#181 > - ThermoFisher/Richard Allan, which calls their substitute Phoenix blue.The problem is, they are being secretive (proprietary) about what dye/reagents are in their solutions. I looked at one time, and couldn't find MSDS on their websites And their flyer doesn't mention the dyes name or CI #. However, the photo on the flyer, at least to me, looks like celestine blue. That's not proof that it IS celestine blue. Could be a close look-alike cousin. > http://www.thermo.com/eThermo/CMA/PDFs/Product/productPDF_6906.pdf > > So look to these companies for more information. Anatech has a newsletter, available on their website, about the hematoxylin shortage (and their Tango blue product, of course). (Not that this will help you with your Celestine blue project, but it will fill you in on the hematoxylin shortage history.) > http://www.anatechltdusa.com/Innovators/Inn08Summer.pdf > > Hope that helps some. > > Peggy A. Wenk, HTL(ASCP)SLS > Schools of Histotechnology > Beaumont Hospital > Royal Oak, MI 48073 > > (Opinions expressed are my own, and not Beaumont Hospitals'.) > > -----Original Message----- From: Bob Richmond > Sent: Sunday, November 06, 2011 3:42 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Celestin blue B (was Help) > > Corrie Vernick writes: > > >>I am currently a histology student at Keiser University. I am doing a project for my routine staining class about Celestine Blue. I've been able to find information on why it was created, the chemical make up, and some of it's uses including the trichrome stain. I am having trouble finding images of slides stained with Celestine Blue. Any additional information about the uses would be helpful as well! Thank you, Corrinne Vernick, Keiser University FL U.S.A.<< > > I don't have access to my library this week, but you can get a good > bit information by Googling celestin blue B. This dye was often used > as a sort of backup or substitute for hematoxylin, particularly in the > old outmoded Pearse stain for pituitary cells. R.D. Lillie as I > remember didn't think much of the dye, and I don't think this dye is a > very good topic for a study such as the one you describe. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Timothy.Morken <@t> ucsfmedctr.org Mon Nov 7 12:09:11 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Nov 7 12:09:21 2011 Subject: [Histonet] My bit of trivia for today - True Blue Message-ID: <8D7C2D242DBD45498006B21122072BF89448A38F@MCINFRWEM003.ucsfmedicalcenter.org> I was listening to the Wait Wait Don't Tell Me radio show this weekend and they had to come up with the derivation of "True Blue" as a saying. Turns out it had to do with blue dye colorfastness. Later they would call it Fast Blue - but that does not convey the later meaning of the phrase.. 'True blue' is supposed to derive from the blue cloth that was made at Coventry, England in the late middle ages. The town's dyers had a reputation for producing material that didn't fade with washing, i.e. it remained 'fast' or 'true'. The phrase 'as true as Coventry blue' originated then and is still used (in Coventry at least). The town's standing was recorded in 1670 by John Ray in the first edition of A Compleat Collection of English Proverbs: "Coventry had formerly the reputation for dying of blues; insomuch that true blue became a Proverb to signifie one that was always the same and like himself." Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org From Lynn.Burton <@t> Illinois.gov Mon Nov 7 08:42:05 2011 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Mon Nov 7 12:46:49 2011 Subject: [Histonet] RE: Zeiss Microm Rotary Microtome HM 325 In-Reply-To: <98CC14B915EBA84B9A326D45CC3C1DEC3AB8FA2A@cbiolabs05.CBiolabs.local> References: <98CC14B915EBA84B9A326D45CC3C1DEC3AB8FA2A@cbiolabs05.CBiolabs.local> Message-ID: <4A6E2CACA1E017408EBA1B9911952CC00645680B93@IL084EXMBX214.illinois.gov> Try Tech One Biomedical at www.techoneweb.com or 1-866-497-3033. They do good work. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lyn Stadler [LStadler@cbiolabs.com] Sent: Friday, November 04, 2011 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Zeiss Microm Rotary Microtome HM 325 Curious if anyone out there has one of these microtomes? We have one that needs repair of the "Trimming Lever". The warranty has expired and I am trying to determine the most cost effective way to deal with it? Pay for the service call or purchase a service contract. Any information/suggestions/comments would be GREATLY appreciated! Lyn Stadler Histology Technician Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Mon Nov 7 12:49:06 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Mon Nov 7 12:49:11 2011 Subject: [Histonet] RE: My bit of trivia for today - True Blue In-Reply-To: <8D7C2D242DBD45498006B21122072BF89448A38F@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF89448A38F@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: Interesting Tim.... My true blue story dates from several years back when I was working up some multiplex IHC staining using 2 HRP substrates. One was DAB and the second was True Blue Substrate from KPL Labs. It worked quite nicely, but is alcohol/xylene soluble so one needs only to wash in water and air dry before coverslipping. Follow this link for more info if interested: http://www.kpl.com/catalog/productdetail.cfm?Catalog_ID=17&Category_ID=440&Product_ID=1015 Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, November 07, 2011 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] My bit of trivia for today - True Blue I was listening to the Wait Wait Don't Tell Me radio show this weekend and they had to come up with the derivation of "True Blue" as a saying. Turns out it had to do with blue dye colorfastness. Later they would call it Fast Blue - but that does not convey the later meaning of the phrase.. 'True blue' is supposed to derive from the blue cloth that was made at Coventry, England in the late middle ages. The town's dyers had a reputation for producing material that didn't fade with washing, i.e. it remained 'fast' or 'true'. The phrase 'as true as Coventry blue' originated then and is still used (in Coventry at least). The town's standing was recorded in 1670 by John Ray in the first edition of A Compleat Collection of English Proverbs: "Coventry had formerly the reputation for dying of blues; insomuch that true blue became a Proverb to signifie one that was always the same and like himself." Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From lldewe <@t> gmail.com Mon Nov 7 12:52:02 2011 From: lldewe <@t> gmail.com (Loralei Dewe) Date: Mon Nov 7 12:52:07 2011 Subject: [Histonet] My bit of trivia for today - True Blue In-Reply-To: <8D7C2D242DBD45498006B21122072BF89448A38F@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF89448A38F@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: That's pretty cool!!! I never knew... Loralei On Mon, Nov 7, 2011 at 10:09 AM, Morken, Timothy < Timothy.Morken@ucsfmedctr.org> wrote: > I was listening to the Wait Wait Don't Tell Me radio show this weekend and > they had to come up with the derivation of "True Blue" as a saying. Turns > out it had to do with blue dye colorfastness. Later they would call it Fast > Blue - but that does not convey the later meaning of the phrase.. > > > 'True blue' is supposed to derive from the blue cloth that was made at > Coventry, England in the late middle ages. The town's dyers had a > reputation for producing material that didn't fade with washing, i.e. it > remained 'fast' or 'true'. The phrase 'as true as Coventry blue' originated > then and is still used (in Coventry at least). The town's standing was > recorded in 1670 by John Ray in the first edition of A Compleat Collection > of English Proverbs: > > "Coventry had formerly the reputation for dying of blues; insomuch that > true blue became a Proverb to signifie one that was always the same and > like himself." > > > Tim Morken > Supervisor, Histology, IPOX > UC San Francisco Medical Center > Box 1656 > 1600 Divisidero St, B217 > San Francisco, CA 94115 > USA > > 415.514.6042 (office) > 415.885.7409 Fax > tim.morken@ucsfmedctr.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cheska91 <@t> hotmail.com Mon Nov 7 13:32:09 2011 From: cheska91 <@t> hotmail.com (cheska mt) Date: Mon Nov 7 13:32:13 2011 Subject: [Histonet] (no subject) Message-ID: Hello I am currently studying to become a histotech. I wanted to know why should the paraffin in the microwave processor be kept at 84 degrees. Thanks From cheska91 <@t> hotmail.com Mon Nov 7 13:38:22 2011 From: cheska91 <@t> hotmail.com (cheska mt) Date: Mon Nov 7 13:38:27 2011 Subject: [Histonet] Quiestion? In-Reply-To: References: Message-ID: From: cheska91@hotmail.com To: histonet@lists.utsouthwestern.edu Subject: Date: Mon, 7 Nov 2011 11:32:09 -0800 Hello I am currently studying to become a histotech. I wanted to know why should the paraffin in the microwave processor be kept at 84 degrees. Thanks From NMP <@t> stowers.org Mon Nov 7 14:05:50 2011 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Mon Nov 7 14:05:55 2011 Subject: [Histonet] RE: My bit of trivia for today - True Blue In-Reply-To: <8D7C2D242DBD45498006B21122072BF89448A38F@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF89448A38F@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <2C40E43D1F7A56408C4463FD245DDDF98B157A34@EXCHMB-02.stowers-institute.org> Thank you for the great explanation along with a little history :-) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, November 07, 2011 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] My bit of trivia for today - True Blue I was listening to the Wait Wait Don't Tell Me radio show this weekend and they had to come up with the derivation of "True Blue" as a saying. Turns out it had to do with blue dye colorfastness. Later they would call it Fast Blue - but that does not convey the later meaning of the phrase.. 'True blue' is supposed to derive from the blue cloth that was made at Coventry, England in the late middle ages. The town's dyers had a reputation for producing material that didn't fade with washing, i.e. it remained 'fast' or 'true'. The phrase 'as true as Coventry blue' originated then and is still used (in Coventry at least). The town's standing was recorded in 1670 by John Ray in the first edition of A Compleat Collection of English Proverbs: "Coventry had formerly the reputation for dying of blues; insomuch that true blue became a Proverb to signifie one that was always the same and like himself." Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Mon Nov 7 14:42:41 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Mon Nov 7 14:42:47 2011 Subject: [Histonet] My bit of trivia for today - True Blue In-Reply-To: <8D7C2D242DBD45498006B21122072BF89448A38F@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF89448A38F@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5B79@PHSXMB30.partners.org> I love Wait, Wait, Don't Tell Me! Interesting bit of trivia. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, November 07, 2011 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] My bit of trivia for today - True Blue I was listening to the Wait Wait Don't Tell Me radio show this weekend and they had to come up with the derivation of "True Blue" as a saying. Turns out it had to do with blue dye colorfastness. Later they would call it Fast Blue - but that does not convey the later meaning of the phrase.. 'True blue' is supposed to derive from the blue cloth that was made at Coventry, England in the late middle ages. The town's dyers had a reputation for producing material that didn't fade with washing, i.e. it remained 'fast' or 'true'. The phrase 'as true as Coventry blue' originated then and is still used (in Coventry at least). The town's standing was recorded in 1670 by John Ray in the first edition of A Compleat Collection of English Proverbs: "Coventry had formerly the reputation for dying of blues; insomuch that true blue became a Proverb to signifie one that was always the same and like himself." Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From megan.french <@t> mcri.edu.au Mon Nov 7 16:52:06 2011 From: megan.french <@t> mcri.edu.au (Megan French) Date: Mon Nov 7 16:52:15 2011 Subject: [Histonet] Immune cells antibody; Histonet Digest, Vol 96, Issue 12 In-Reply-To: References: Message-ID: Message: 12 Date: Mon, 7 Nov 2011 09:48:01 +0100 (CET) From: "Carmen Maria Garcia Pascual" Subject: [Histonet] Immune cells antibody To: histonet@lists.utsouthwestern.edu Message-ID: <2427631794carmaga6@uv.es> Content-Type: text/plain; charset="ISO-8859-1" Hi everyone: I want to detect immune cells (natural killers) in mouse's decidua, I wonder if somebody knows a good antibody for that. thanks!!! Carmen --- I would try a CD1d antibody. Sorry, cant provide you with a supplier. Megan French Murdoch Children's Research Institute, Australia megan.french@mcri.edu.au ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From AnthonyH <@t> chw.edu.au Mon Nov 7 16:56:34 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood (SCHN)) Date: Mon Nov 7 16:56:49 2011 Subject: [Histonet] My bit of trivia for today - True Blue In-Reply-To: References: <8D7C2D242DBD45498006B21122072BF89448A38F@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A034F4@xmdb02.nch.kids> In Australia, "True Blue" has other meanings >From the website: http://www.malleeboy.com/music/true_blue_21.html An Australian country & western singer named John Williamson wrote a song entitled "True Blue" Definition of "True Blue": "Steadfast loyal Australian who displays the Aussie ideals of a fair go for all, mateship, having a go, and solving problems." (maybe a bit over the top). The lyrics (& you can listen to part of the song on the above website) are: Hey True Blue, don't say you've gone Say you've knocked off for a smoko And you'll be back later on Hey True Blue, Hey True Blue Give it to me straight Face to face Are you really disappearing, Just another dying race, Hey True Blue. True Blue, is it me and you? Is it Mum and Dad, is it a cockatoo? Is it standing by your mate When he's in a fight? Or will she be right? True Blue, I'm asking you... Hey True Blue, can you bear the load? Will you tie it up with wire, Just to keep the show on the road? Hey True Blue, Hey True Blue, now be Fair Dinkum Is your heart still there? If they sell us out like sponge cake Do you really care? Hey True Blue. If you would like further translation please ask!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Loralei Dewe Sent: Tuesday, 8 November 2011 5:52 AM To: Morken, Timothy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] My bit of trivia for today - True Blue That's pretty cool!!! I never knew... Loralei On Mon, Nov 7, 2011 at 10:09 AM, Morken, Timothy < Timothy.Morken@ucsfmedctr.org> wrote: > I was listening to the Wait Wait Don't Tell Me radio show this weekend > and they had to come up with the derivation of "True Blue" as a > saying. Turns out it had to do with blue dye colorfastness. Later they > would call it Fast Blue - but that does not convey the later meaning of the phrase.. > > > 'True blue' is supposed to derive from the blue cloth that was made at > Coventry, England in the late middle ages. The town's dyers had a > reputation for producing material that didn't fade with washing, i.e. > it remained 'fast' or 'true'. The phrase 'as true as Coventry blue' > originated then and is still used (in Coventry at least). The town's > standing was recorded in 1670 by John Ray in the first edition of A > Compleat Collection of English Proverbs: > > "Coventry had formerly the reputation for dying of blues; insomuch > that true blue became a Proverb to signifie one that was always the > same and like himself." > > > Tim Morken > Supervisor, Histology, IPOX > UC San Francisco Medical Center > Box 1656 > 1600 Divisidero St, B217 > San Francisco, CA 94115 > USA > > 415.514.6042 (office) > 415.885.7409 Fax > tim.morken@ucsfmedctr.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From victor <@t> pathology.washington.edu Mon Nov 7 17:10:45 2011 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Nov 7 17:11:01 2011 Subject: [Histonet] Specimen Couriers Message-ID: <4EB86575.7020505@pathology.washington.edu> One of our Lab Managers asked me to throw this topic to the Histonet. Who is transporting surgical specimens between sites and are you using a tracking system, if so what is it? Also, do you use a hired courier or have a department courier system? I will forward all replies to the manager. Thanks Victor -- Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From max_histo_00 <@t> yahoo.it Tue Nov 8 01:40:59 2011 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Tue Nov 8 01:41:04 2011 Subject: [Histonet] paraffin temperature In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC061A18@JERRY.Gia.com> References: <3966F8F4-56EC-4299-9B98-E8B11A87F510@mtsac.edu> <5A33C952BB67F4468AF1F36D739212BC061A18@JERRY.Gia.com> Message-ID: <1320738059.73496.YahooMailNeo@web29614.mail.ird.yahoo.com> There is a very simple method to establish the real melting point of your paraffin. You fill with melted paraffin a glass capillary? and you allow it to solidify. Then you dip the capillary into a porcelain capsule full of water in which the bulb of a thermometer is plunged. You slowly heat the water and you read the temperature at which the paraffin,? contained in the capillary , melts. Best Regards, Massimo Tosi ________________________________ Da: Amber McKenzie A: Jennifer MacDonald ; Zoe rosa Cc: "" Inviato: Luned? 7 Novembre 2011 15:33 Oggetto: RE: [Histonet] paraffin temperature I have my paraffin chambers set at 60 degrees (embedding center and processor) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, November 04, 2011 11:00 PM To: Zoe rosa Cc: Subject: Re: [Histonet] paraffin temperature It depends on the paraffin. The melting point is usually on the bag. Sent from my iPhone On Nov 4, 2011, at 8:28 PM, Zoe rosa wrote: > > Hello all, > > I need help with something basic: What is the melting temperature for paraffin? > > I appreciate your help, > > Thanks, Ismael? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Tue Nov 8 02:42:57 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Nov 8 02:43:21 2011 Subject: [Histonet] paraffin temperature In-Reply-To: <1320738059.73496.YahooMailNeo@web29614.mail.ird.yahoo.com> References: <3966F8F4-56EC-4299-9B98-E8B11A87F510@mtsac.edu> <5A33C952BB67F4468AF1F36D739212BC061A18@JERRY.Gia.com> <1320738059.73496.YahooMailNeo@web29614.mail.ird.yahoo.com> Message-ID: Our paraffin melts at 60C with pressure from a vacuum. I don't know if there's actually enough pressure to affect the melting point of the paraffin, but we always check the thermometer hanging inside the oven to make sure it stays at 60C. If you have a certain brand you're not sure of, I would check with the company. I just looked on Fisher Scientific's site and they listed what the melting point was for their paraffin. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron From talulahgosh <@t> gmail.com Tue Nov 8 02:46:41 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Nov 8 02:46:45 2011 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: Because your boss said to leave it at 84 degrees? Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that?s beautiful. --Ron Swanson From Martin.Hasselblatt <@t> ukmuenster.de Tue Nov 8 03:38:58 2011 From: Martin.Hasselblatt <@t> ukmuenster.de (Hasselblatt, Martin) Date: Tue Nov 8 03:39:07 2011 Subject: [Histonet] Automated Slide Stainer and Coverslipper: Foil or Glass? Message-ID: Dear list members, we have funding to buy an automated slide stainer and coverslipper and are considering to go for the Tissue-Tek? Prisma. If you had the choice: Would you opt for a glass coverslipper or a foil coverslipper? Feedback of pathologists on the optical quality of the foiled slides as well your technical experiences with maintenance (we heard rumors the glass version is running less stable) are most welcome. Please take into account that we plan to archive slides "infinitely" and will not discard them after 5 years. Thank you very much for your help! All the best from Germany, Martin ________________________________________ Martin Hasselblatt, M.D. Professor of Neuropathology Institute of Neuropathology Universit?tsklinikum M?nster 48149 M?nster, Germany From Donna.Willis <@t> baylorhealth.edu Tue Nov 8 07:20:54 2011 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Tue Nov 8 07:21:04 2011 Subject: [Histonet] Quiestion? In-Reply-To: References: Message-ID: <3FA597486B013249A2FC8EE113CBCC02185BAEB6CF@BHDAEXVM33.bhcs.pvt> The purpose of getting the paraffin up to 84C is to evaporate out the Isopropanol that is used instead of Xylene. This does not mean that the paraffin should be kept at that temperature when it is not being used for the infiltration step. It should be in an oven or a paraffin pot at a temperature no higher than 65C then allow the microwave to heat it up to 84C. This also depends on the type of microwave that you are using. If the unit has a vacuum system that is being used during the infiltration step then the temperature does not have reach 84C to evaporate out the IPA. Read your user manual that from the manufacture, it should help. Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cheska mt Sent: Monday, November 07, 2011 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Quiestion? From: cheska91@hotmail.com To: histonet@lists.utsouthwestern.edu Subject: Date: Mon, 7 Nov 2011 11:32:09 -0800 Hello I am currently studying to become a histotech. I wanted to know why should the paraffin in the microwave processor be kept at 84 degrees. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From cpyse <@t> x-celllab.com Tue Nov 8 10:03:12 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Nov 8 10:03:21 2011 Subject: [Histonet] Automated Slide Stainer and Coverslipper: Foil or Glass? In-Reply-To: References: Message-ID: <002401cc9e2f$eb2761e0$c17625a0$@com> Martin We currently have a Leica CV35030, which is a glass cover slipper. My 15 Pathologists prefer the glass covers slips for the optical quality. We are required to archive our slides for 20 years, which is another reason we chose glass cover slips. The Leica CV5030 if cleaned nightly and maintained properly is a work horse, with only small problems that are easily fixed within the lab. Cindy Cindy Pyse, CLT, HT(ASCP) Laboratory Manager X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hasselblatt, Martin Sent: Tuesday, November 08, 2011 4:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Slide Stainer and Coverslipper: Foil or Glass? Dear list members, we have funding to buy an automated slide stainer and coverslipper and are considering to go for the Tissue-Tek? Prisma. If you had the choice: Would you opt for a glass coverslipper or a foil coverslipper? Feedback of pathologists on the optical quality of the foiled slides as well your technical experiences with maintenance (we heard rumors the glass version is running less stable) are most welcome. Please take into account that we plan to archive slides "infinitely" and will not discard them after 5 years. Thank you very much for your help! All the best from Germany, Martin ________________________________________ Martin Hasselblatt, M.D. Professor of Neuropathology Institute of Neuropathology Universit?tsklinikum M?nster 48149 M?nster, Germany _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alvarez092703 <@t> hotmail.com Tue Nov 8 10:05:12 2011 From: alvarez092703 <@t> hotmail.com (jose and leticia alvarez) Date: Tue Nov 8 10:05:16 2011 Subject: [Histonet] micro wave processors Message-ID: I am in a mirotomy class, and we had a discussion about mircorwave processing. We need an accurate temperature. Freida states it should be at 84 degrees however we think she is incorrect. please respond asap. thank you leticia alvarez From sbreeden <@t> nmda.nmsu.edu Tue Nov 8 10:10:53 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Nov 8 10:11:02 2011 Subject: [Histonet] Automated Slide Stainer and Coverslipper: Foil orGlass? In-Reply-To: <002401cc9e2f$eb2761e0$c17625a0$@com> References: <002401cc9e2f$eb2761e0$c17625a0$@com> Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFBD1@nmdamailsvr.nmda.ad.nmsu.edu> I know I've been around a l-o-n-g time, but what the heck is a "foiled" slide? That's a new one on me! Rats! Foiled again! From lblazek <@t> digestivespecialists.com Tue Nov 8 10:13:25 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Nov 8 10:13:39 2011 Subject: [Histonet] Automated Slide Stainer and Coverslipper: Foil orGlass? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DFBD1@nmdamailsvr.nmda.ad.nmsu.edu> References: <002401cc9e2f$eb2761e0$c17625a0$@com> <4D14F0FC9316DD41972D5F03C070908B051DFBD1@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E39127401B496@IBMB7Exchange.digestivespecialists.com> You would think that if it's foiled it would be hard to see with the microscope. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, November 08, 2011 11:11 AM To: Cynthia Pyse; Hasselblatt, Martin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automated Slide Stainer and Coverslipper: Foil orGlass? I know I've been around a l-o-n-g time, but what the heck is a "foiled" slide? That's a new one on me! Rats! Foiled again! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nelsonrnch <@t> verizon.net Tue Nov 8 11:22:01 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Tue Nov 8 11:22:05 2011 Subject: [Histonet] Quiestion? In-Reply-To: <3FA597486B013249A2FC8EE113CBCC02185BAEB6CF@BHDAEXVM33.bhcs.pvt> References: <3FA597486B013249A2FC8EE113CBCC02185BAEB6CF@BHDAEXVM33.bhcs.pvt> Message-ID: <1320772921.17779.YahooMailNeo@web84520.mail.ne1.yahoo.com> I currently use a TBS for a GI lab and my temps for paraffin do not exceed 67 C during processing. I know it is recommended to be at 84 C, but at that temp it would fry everything and chatter was a serious issue even after soaking. ? THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ? ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. ________________________________ From: "Willis, Donna G." To: cheska mt ; "histonet@lists.utsouthwestern.edu" Sent: Tuesday, November 8, 2011 5:20 AM Subject: RE: [Histonet] Quiestion? The purpose of getting the paraffin up to 84C is to evaporate out the Isopropanol that is used instead of Xylene.? This does not mean that the paraffin should be kept at that temperature when it is not being used for the infiltration step.? It should be in an oven or a paraffin pot at a temperature no higher than 65C then allow the microwave to heat it up to 84C.? This also depends on the type of microwave that you are using.? If the unit has a vacuum system that is being used during the infiltration step then the temperature does not have reach 84C to evaporate out the IPA.? Read your user manual that from the manufacture, it should help. Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cheska mt Sent: Monday, November 07, 2011 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Quiestion? From: cheska91@hotmail.com To: histonet@lists.utsouthwestern.edu Subject: Date: Mon, 7 Nov 2011 11:32:09 -0800 Hello ? ? I am currently studying to become a histotech. I wanted to know why should the paraffin in the microwave processor be kept at 84 degrees. Thanks ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Tue Nov 8 11:26:11 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Nov 8 11:26:15 2011 Subject: [Histonet] Melting paraffin Message-ID: <1320773171.37132.YahooMailNeo@web39408.mail.mud.yahoo.com> ?Hi Zoe- The melting point of your paraffin depends on the paraffin!? Each type has a different additives so the?melting point vs high? temp point varies?and too much heat can break down the structure.? The package should have the correct set point for the product it contains--generally 3-5 degrees above the actual melting point. For example, Tissue Tek's VIP has a melting point of 56C and a high temp stability of 65C, but Paraplast Plus has a more narrow window with a melt at 56C but a high temp stability of 62C. (pulled from the internet so pardon if I'm off a bit). Hope this helps! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From nelsonrnch <@t> verizon.net Tue Nov 8 11:30:13 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Tue Nov 8 11:30:17 2011 Subject: [Histonet] micro wave processors In-Reply-To: References: Message-ID: <1320773413.77948.YahooMailNeo@web84502.mail.ne1.yahoo.com> You will always have a recommended protocol. It is up to us as HT/HTL? to determine what exactly will work. That is where TROUBLE SHOOTING falls in. As I posted earlier, I use a certain type of microwave processor and because the recommended temps did not work on the type of tissue I was processing. I had to trouble shoot for what would work. Again, my paraffin temps do not exceed 67C. THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ? ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. ________________________________ From: jose and leticia alvarez To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 8, 2011 8:05 AM Subject: [Histonet] micro wave processors I am in a mirotomy class, and we had a discussion about mircorwave processing.? We need an accurate temperature.? Freida states it should be at 84 degrees however we think she is incorrect.? please respond asap. thank you leticia alvarez? ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nelsonrnch <@t> verizon.net Tue Nov 8 11:31:54 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Tue Nov 8 11:32:04 2011 Subject: [Histonet] Re: Paraffin temperatures In-Reply-To: <000001cc9e3b$7f722d70$7e568850$@bresnan.net> References: <000001cc9e3b$7f722d70$7e568850$@bresnan.net> Message-ID: <1320773514.76444.YahooMailNeo@web84514.mail.ne1.yahoo.com> Thanks Gayle for the tip, wouldn't hurt to improve. I will test it and post the results. THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ? ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. ________________________________ From: gayle callis To: nelsonrnch@verizon.net Sent: Tuesday, November 8, 2011 9:26 AM Subject: Paraffin temperatures You are frying your biopsies at 67C too.? Keep you paraffin temperature no more than 60C, closer to melting point of paraffin.? Whoever told you to use 84C was WRONG!!!?? That is hot beyond belief, and they did not know what they were doing.?? ? Gayle M. Callis HTL/HT/MT(ASCP) From joelleweaver <@t> hotmail.com Tue Nov 8 11:42:41 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Nov 8 11:42:46 2011 Subject: [Histonet] micro wave processors In-Reply-To: <1320773413.77948.YahooMailNeo@web84502.mail.ne1.yahoo.com> References: , <1320773413.77948.YahooMailNeo@web84502.mail.ne1.yahoo.com> Message-ID: >From my research I have found that the molecular water (polar) content and insulating fat content of the tissue types are added considerations. If you look at the physics and how microwaves penetrate and generate heat, this really helps in customizing schedules for different tissues and using its properties and effects to best advantage. The goods news is that the MW processors have more developed software that allows a wide manipulation ( in my experience) for processing protocols. Still, as you mentioned troubleshooting is as always required. Being newer technology, there are some implementation hurdles, but I have seen it work sucessfully for at least a large volume of various tissue types, signifigantly decreasing turn around times and leveling out workflow that allows staffing to be adjusted in favorable ways. Some labs I have worked at always seem to have certain tissues though that they have not been able to optimize a suitable protocol for processing with microwave assistance so far. Still in the refinement and development stages in many cases.Joelle Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > Date: Tue, 8 Nov 2011 09:30:13 -0800 > From: nelsonrnch@verizon.net > To: alvarez092703@hotmail.com > Subject: Re: [Histonet] micro wave processors > CC: Histonet@lists.utsouthwestern.edu > > You will always have a recommended protocol. It is up to us as HT/HTL to determine what exactly will work. That is where TROUBLE SHOOTING falls in. > As I posted earlier, I use a certain type of microwave processor and because the recommended temps did not work on the type of tissue I was processing. I had to trouble shoot for what would work. > Again, my paraffin temps do not exceed 67C. > > THANK YOU, > > PATTI RUBEN-NELSON H.T.(ASCP) > PNP LABORATORY CONSULTANTS > SUPERVISOR/DGC > P.O. BOX 412 > CABAZON, CA. 92230 > cell (909) 841-9761 > nelsonrnch@verizon.net > > > CONFIDENTIALITY NOTICE: > This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants > and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable > law. Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful. If you are not > the addressee, please promptly delete this message and notify the sender of > the delivery error by e-mail or you may call 909-841-9761. > > > ________________________________ > From: jose and leticia alvarez > To: histonet@lists.utsouthwestern.edu > Sent: Tuesday, November 8, 2011 8:05 AM > Subject: [Histonet] micro wave processors > > > > > > I am in a mirotomy class, and we had a discussion about mircorwave processing. We need an accurate temperature. Freida states it should be at 84 degrees however we think she is incorrect. please respond asap. thank you leticia alvarez _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Nov 8 11:44:34 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Nov 8 11:44:38 2011 Subject: [Histonet] micro wave processors In-Reply-To: <1320773413.77948.YahooMailNeo@web84502.mail.ne1.yahoo.com> References: , <1320773413.77948.YahooMailNeo@web84502.mail.ne1.yahoo.com> Message-ID: 67 is too hot for vacuum-pressure assisted conventional processing. MW processors use up to 84 to evaporate the IPA, but the time periods are often as little as 5 minutes. Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > Date: Tue, 8 Nov 2011 09:30:13 -0800 > From: nelsonrnch@verizon.net > To: alvarez092703@hotmail.com > Subject: Re: [Histonet] micro wave processors > CC: Histonet@lists.utsouthwestern.edu > > You will always have a recommended protocol. It is up to us as HT/HTL to determine what exactly will work. That is where TROUBLE SHOOTING falls in. > As I posted earlier, I use a certain type of microwave processor and because the recommended temps did not work on the type of tissue I was processing. I had to trouble shoot for what would work. > Again, my paraffin temps do not exceed 67C. > > THANK YOU, > > PATTI RUBEN-NELSON H.T.(ASCP) > PNP LABORATORY CONSULTANTS > SUPERVISOR/DGC > P.O. BOX 412 > CABAZON, CA. 92230 > cell (909) 841-9761 > nelsonrnch@verizon.net > > > CONFIDENTIALITY NOTICE: > This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants > and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable > law. Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful. If you are not > the addressee, please promptly delete this message and notify the sender of > the delivery error by e-mail or you may call 909-841-9761. > > > ________________________________ > From: jose and leticia alvarez > To: histonet@lists.utsouthwestern.edu > Sent: Tuesday, November 8, 2011 8:05 AM > Subject: [Histonet] micro wave processors > > > > > > I am in a mirotomy class, and we had a discussion about mircorwave processing. We need an accurate temperature. Freida states it should be at 84 degrees however we think she is incorrect. please respond asap. thank you leticia alvarez _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Tue Nov 8 11:46:17 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Nov 8 11:46:22 2011 Subject: [Histonet] Staffing levels... Message-ID: <1320774377.52685.YahooMailNeo@web39420.mail.mud.yahoo.com> Hi Guys- ? We know this is a sensitive subject!? I realize my response, below, doesn't answer your question--you'll get the numbers from other Netters.? Please be cautious in using them out of context for your lab and your staffers!! ??depend on so many moving parts that in a small lab (like yours)?each set of tasks may take more time than in a huge production style?lab.? Stock numbers are a good place to start.? For a little investment of time, you can do productivity numbers for your own staff that mean more for your manager than the stock numbers.?These numbers ? If you're a solo tech who accessions, grosses, processes, cuts, stains by hand and turns out with specials and then files everything?ALL BY YOURSELF, the max is about 50 blocks a day if you're a ROCK STAR.? If you work in a situation where there are no transitions between tasks in a larger lab--sure a good tech can cut 180+ biopsy blocks a day but they'll blow out their shoulders in a few short years.? Perspective is an important part of the picture you give to those who are asking. ? I had a tech who was WICKED fast and everyone thought she was the high-producer.? She made 1-2 minor mistakes a day that required time to correct.? The slower tech made NO mistakes...so in the end her productivy rate was highter than the speed demon.? ? My three cents! ? Cheryl? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From tkngflght <@t> yahoo.com Tue Nov 8 11:58:38 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Nov 8 11:58:42 2011 Subject: [Histonet] Blade choice Message-ID: <1320775118.13898.YahooMailNeo@web39413.mail.mud.yahoo.com> Like all other things 'histology', blade choice depends on so many different things!? Tissue type, fixation, paraffin type, mircrotome and associated blade holder, humidity in the workroom, etc.? I know there are people who will poo-poo this opinion--so try it for yourself! ? Contact your vendors and ask for samples of every blade you think you'll like.? They'll send samples from 2 blades to a 10-blade mini pack.? Then sit down and TRY them.? You WILL have one blade work significantly better than the others--you may also want a second type for hard or decal tissues.? ? If you're concerned about cost, also consider the extra time your techs need to produce a GOOD slide on an blade that isn't optimal for your lab.? Tech time trumps blade cost every time. One 'cut thru' block could cost your patient a correct diagnosis and the lab a chunk to make it right. ? Most techs have a favorite, but most techs also don't?care what blade they use as long as they don't have to struggle to produce good work.? Nothing worse than facing a tiny pediatric kidney core knowing you have to produce 15+ slides on a crappy blade.? My antiperspirant isn't up to that task!! ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From AHutton <@t> dh.org Tue Nov 8 11:57:14 2011 From: AHutton <@t> dh.org (Hutton, Allison) Date: Tue Nov 8 11:59:36 2011 Subject: [Histonet] release of tissues back to patient Message-ID: <38A56C4F4630D348A50B3720409270870E0FE473@dhmail.dhorg.org> We had a question come up regarding giving patient's back their placentas (patient's request) after delivery. Our general rule is not to return tissues, except for religious reasons. We are now trying to come up with a concrete SOP for (or not) returning tissues. I was curious what other institutions are doing in regards to this topic. I know laws vary by location but I am looking for a general idea. Thank you in advance, Allison From joelleweaver <@t> hotmail.com Tue Nov 8 12:06:49 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Nov 8 12:06:54 2011 Subject: [Histonet] Staffing levels... In-Reply-To: <1320774377.52685.YahooMailNeo@web39420.mail.mud.yahoo.com> References: <1320774377.52685.YahooMailNeo@web39420.mail.mud.yahoo.com> Message-ID: I agree that in house developed metrics and benchmarks are always the most useful. As you pointed out, just productivity measures and metrics without task analysis and consideration of work flow should be used with some caution. Also agree that speed pales when it is compromised by errors and a reduction in quality. With experience, you learn how to manage these conflicting pressures and I would add that a good supervisor can help their staff in managing this. I have seen where those who do not perform these tasks, (such as microtomy), or fully understand the work flow or process of histology in any given lab , will sometimes try to use such numbers, and this can result to encourage speed, but not accuracy and quality- that is the danger so to speak. Most frequently I have encountered this myself when I have a supervisor or manager who is not a histologist, and/or has never spent any time on any histology bench. However regardess of background, the more insightful will soon see that this will not yield the overall desired results, though it may make productivity numbers go up in the short run, other metrics such as errors, mislabels, recuts, etc., will tend to spike. Just speaks for wisdom, experience and taking the time to develop measures that actually work, and are directed at broader organizational goals- yes, this takes a little more time and effort for sure. Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > Date: Tue, 8 Nov 2011 09:46:17 -0800 > From: tkngflght@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Staffing levels... > > Hi Guys- > > We know this is a sensitive subject! I realize my response, below, doesn't answer your question--you'll get the numbers from other Netters. Please be cautious in using them out of context for your lab and your staffers!! > depend on so many moving parts that in a small lab (like yours) each set of tasks may take more time than in a huge production style lab. > Stock numbers are a good place to start. For a little investment of time, you can do productivity numbers for your own staff that mean more for your manager than the stock numbers. These numbers > > If you're a solo tech who accessions, grosses, processes, cuts, stains by hand and turns out with specials and then files everything ALL BY YOURSELF, the max is about 50 blocks a day if you're a ROCK STAR. If you work in a situation where there are no transitions between tasks in a larger lab--sure a good tech can cut 180+ biopsy blocks a day but they'll blow out their shoulders in a few short years. Perspective is an important part of the picture you give to those who are asking. > > I had a tech who was WICKED fast and everyone thought she was the high-producer. She made 1-2 minor mistakes a day that required time to correct. The slower tech made NO mistakes...so in the end her productivy rate was highter than the speed demon. > > My three cents! > > Cheryl > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT Tech at a time. > 281.852.9457 Office > 800.756.3309 Phone & Fax > admin@fullstaff.org > > Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Nov 8 12:11:35 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Nov 8 12:11:40 2011 Subject: [Histonet] release of tissues back to patient In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE473@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870E0FE473@dhmail.dhorg.org> Message-ID: Ohio has laws regarding this release- rather recent legislation. This gives rights to fetal tissue that extends earlier in gestation than previously, and by my recollection expands upon the legal rights of burial, autopsy provides to a fetus in previous statutes. I would search your state's revised code, and I know that Ohio's basically lays out the requirements for facilities in the language of its legislation and statute, and this basically writes the SOP for you. If you need to do this by your state's law, then you can use a lot of the language contained and make sure that you are follow the legal obligations in your form(s). Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > Date: Tue, 8 Nov 2011 12:57:14 -0500 > From: AHutton@dh.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] release of tissues back to patient > > We had a question come up regarding giving patient's back their placentas (patient's request) after delivery. Our general rule is not to return tissues, except for religious reasons. We are now trying to come up with a concrete SOP for (or not) returning tissues. I was curious what other institutions are doing in regards to this topic. I know laws vary by location but I am looking for a general idea. > Thank you in advance, > Allison > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Tue Nov 8 12:12:25 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Nov 8 12:12:28 2011 Subject: [Histonet] Supervisor Position Available - Pasadena, CA Message-ID: <57BE698966D5C54EAE8612E8941D76830C77143F@EXCHANGE3.huntingtonhospital.com> Huntington Hospital in Pasadena, CA has a supervisor's position available immediately. The position oversees Histology and the grossing room with a total staff of 11. The hospital provides full benefits, including a 401(b) plan. Huntington is an awesome hospital to work for. The position should be posted tomorrow. Please apply online at: www.huntingtonhospital.com. Feel free to email me if you would like additional information. Laurie Colbert From one_angel_secret <@t> yahoo.com Tue Nov 8 12:57:22 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Nov 8 12:57:26 2011 Subject: [Histonet] release of tissues back to patient In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE473@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870E0FE473@dhmail.dhorg.org> Message-ID: <1320778642.73916.YahooMailNeo@web112318.mail.gq1.yahoo.com> At most places where I have either served as supervisor or tech, this is what we did. ? Followed the CAP guidelines for retention of wet tissues. Which is 2 weeks after sign out. ? http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=policies%2Fpolicy_appPP.html&_state=maximized&_pageLabel=cntvwr ? Ive seen request for placenta's, legs etc along with the most common, a gall stone. typically things like gallstones, hardware, things we could render non bio hazardous to humans we would let go with a signed release form (after proper identification after the 2 week guideline) HIPPA rule. ? Antthing that would be considered an "organ" such as a placenta ( and yes, to my knowledge it is still illegal for someone to eat them in this country. Dont laugh, some religions do this and they ask for this purpose) I have always refered them to use a morturary service for getting the organ back. Some religions require this for burial purposes. placenta, leg, etc ? While we must be sensative to the patients religious views, we also must be sensative to allowing any hazardous material being released into the public. ? Hope this helps ? Kim Donadio ? ? ________________________________ From: "Hutton, Allison" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 8, 2011 12:57 PM Subject: [Histonet] release of tissues back to patient We had a question come up regarding giving patient's back their placentas (patient's request) after delivery.? Our general rule is not to return tissues, except for religious reasons.? We are now trying to come up with a concrete SOP for (or not) returning tissues.? I was curious what other institutions are doing in regards to this topic.? I know laws vary by location but I am looking for a general idea. Thank you in advance, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Tue Nov 8 14:27:53 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Nov 8 14:27:58 2011 Subject: [Histonet] Re: My bit of trivia for today - True Blue Message-ID: Tim Morken notes: >>I was listening to the Wait Wait Don't Tell Me radio show this weekend and they had to come up with the derivation of "True Blue" as a saying. Turns out it had to do with blue dye colorfastness. Later they would call it Fast Blue - but that does not convey the later meaning of the phrase.. >> 'True blue' is supposed to derive from the blue cloth that was made at Coventry, England in the late middle ages. The town's dyers had a reputation for producing material that didn't fade with washing, i.e. it remained 'fast' or 'true'. The phrase 'as true as Coventry blue' originated then and is still used (in Coventry at least). The town's standing was recorded in 1670 by John Ray in the first edition of A Compleat Collection of English Proverbs: >>"Coventry had formerly the reputation for dying of blues; insomuch that true blue became a Proverb to signifie one that was always the same and like himself."<< ********************************************************************** Coventry was a center of woad production and dyeing. Woad (Isatis tinctoria) is one of several plants that contain the dye precursor indican. Dyeing with indigo is a complex process involving oxidation and reduction of the blue pigment. See http://www.woad.org.uk/html/britain.html Coventry was of course Lady Godiva's home town. In World War 2 the Germans bombed Coventry, destroying its ancient Anglican cathedral, which was rebuilt after the war. To this day the Anglican cathedral at Salisbury uses blue vestments and altar cloths (Sarum usage - Sarum is the Latin name of Salisbury.) Bob Richmond Samurai Pathologist Knoxville TN From gagnone <@t> KGH.KARI.NET Tue Nov 8 14:32:31 2011 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Tue Nov 8 14:32:41 2011 Subject: [Histonet] Training and Competency Assessment for H&E Slide Review Message-ID: Yes Victoria, Canadian laboratories can participate in HistoQIP as well. Eric Gagnon MLT Histology Laboratory Kingston General Hospital, Kingston, Ontario, Canada From rsrichmond <@t> gmail.com Tue Nov 8 14:35:03 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Nov 8 14:35:07 2011 Subject: [Histonet] Re: release of tissues back to patient Message-ID: Allison Hutton asks: >>We had a question come up regarding giving patients back their placentas (patient's request) after delivery. Our general rule is not to return tissues, except for religious reasons. We are now trying to come up with a concrete SOP for (or not) returning tissues. I was curious what other institutions are doing in regards to this topic. I know laws vary by location but I am looking for a general idea.<< This is a complicated topic. There's a bizarre fad out there for women's eating their babies' placentas. Obviously they don't want them fixed in formalin. I actually had such a request once. Highly observant Jews may require burial of resected tissue in a Jewish cemetery, and native Americans may have similar requirements. I've also had requests for return of amputated limbs for burial in a rural family cemetery. Requests like this are best handled with the help of a funeral director. I think the Joint Commission (or whatever they're called this week) has banned souvenir gallstones and tonsils. Remember if you simply must hand a patient fixed tissue, transfer it from formaldehyde to 70% alcohol - much less toxic. Bob Richmond Samurai Pathologist Knoxville TN From joelleweaver <@t> hotmail.com Tue Nov 8 14:54:44 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Tue Nov 8 14:54:48 2011 Subject: [Histonet] release of tissues back to patient Message-ID: CAP retention guidelines of course, but some states have laws allowing the parents to receive tissue, of course in 70 percent ETOH, for the purposes of religous rites and burial. Waiver/form needed. This extends/allows for legal burial rights previously extended to fetuses of further gestation. Laws will vary by state or even may not exist in some Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Kim Donadio Date: Tue, 8 Nov 2011 18:57:22 To: ; Subject: Re: [Histonet] release of tissues back to patient At most places where I have either served as supervisor or tech, this is what we did. ? Followed the CAP guidelines for retention of wet tissues. Which is 2 weeks after sign out. ? http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=policies%2Fpolicy_appPP.html&_state=maximized&_pageLabel=cntvwr ? Ive seen request for placenta's, legs etc along with the most common, a gall stone. typically things like gallstones, hardware, things we could render non bio hazardous to humans we would let go with a signed release form (after proper identification after the 2 week guideline) HIPPA rule. ? Antthing that would be considered an "organ" such as a placenta ( and yes, to my knowledge it is still illegal for someone to eat them in this country. Dont laugh, some religions do this and they ask for this purpose) I have always refered them to use a morturary service for getting the organ back. Some religions require this for burial purposes. placenta, leg, etc ? While we must be sensative to the patients religious views, we also must be sensative to allowing any hazardous material being released into the public. ? Hope this helps ? Kim Donadio ? ? ________________________________ From: "Hutton, Allison" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 8, 2011 12:57 PM Subject: [Histonet] release of tissues back to patient We had a question come up regarding giving patient's back their placentas (patient's request) after delivery.? Our general rule is not to return tissues, except for religious reasons.? We are now trying to come up with a concrete SOP for (or not) returning tissues.? I was curious what other institutions are doing in regards to this topic.? I know laws vary by location but I am looking for a general idea. Thank you in advance, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Nov 8 14:57:05 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Tue Nov 8 14:57:11 2011 Subject: [Histonet] micro wave processors Message-ID: I should add, believe includes placental tissue or POC Sent from my Verizon Wireless BlackBerry -----Original Message----- From: joelle weaver Date: Tue, 8 Nov 2011 17:42:41 To: ; ; Subject: RE: [Histonet] micro wave processors >From my research I have found that the molecular water (polar) content and insulating fat content of the tissue types are added considerations. If you look at the physics and how microwaves penetrate and generate heat, this really helps in customizing schedules for different tissues and using its properties and effects to best advantage. The goods news is that the MW processors have more developed software that allows a wide manipulation ( in my experience) for processing protocols. Still, as you mentioned troubleshooting is as always required. Being newer technology, there are some implementation hurdles, but I have seen it work sucessfully for at least a large volume of various tissue types, signifigantly decreasing turn around times and leveling out workflow that allows staffing to be adjusted in favorable ways. Some labs I have worked at always seem to have certain tissues though that they have not been able to optimize a suitable protocol for processing with microwave assistance so far. Still in the refinement and development stages in many cases.Joelle Joelle Weaver MAOM, BA, (HTL) ASCP ? http://www.linkedin.com/in/joelleweaver ?> Date: Tue, 8 Nov 2011 09:30:13 -0800 > From: nelsonrnch@verizon.net > To: alvarez092703@hotmail.com > Subject: Re: [Histonet] micro wave processors > CC: Histonet@lists.utsouthwestern.edu > > You will always have a recommended protocol. It is up to us as HT/HTL? to determine what exactly will work. That is where TROUBLE SHOOTING falls in. > As I posted earlier, I use a certain type of microwave processor and because the recommended temps did not work on the type of tissue I was processing. I had to trouble shoot for what would work. > Again, my paraffin temps do not exceed 67C. > > THANK YOU, >? > PATTI RUBEN-NELSON? H.T.(ASCP) > PNP LABORATORY CONSULTANTS > SUPERVISOR/DGC > P.O. BOX 412 > CABAZON, CA. 92230 > cell (909) 841-9761 > nelsonrnch@verizon.net >? >? > CONFIDENTIALITY NOTICE: > This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants >? and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable > law.? Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful.? If you are not > the addressee, please promptly delete this message and notify the sender of > the delivery error by e-mail or you may call? 909-841-9761. > > > ________________________________ > From: jose and leticia alvarez > To: histonet@lists.utsouthwestern.edu > Sent: Tuesday, November 8, 2011 8:05 AM > Subject: [Histonet] micro wave processors > > > > > > I am in a mirotomy class, and we had a discussion about mircorwave processing.? We need an accurate temperature.? Freida states it should be at 84 degrees however we think she is incorrect.? please respond asap. thank you leticia alvarez????????????????????????? _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ?????????????????????????????????????? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Nov 8 14:58:10 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Tue Nov 8 14:58:14 2011 Subject: [Histonet] micro wave processors Message-ID: I should add, believe includes placental tissue or POC Sent from my Verizon Wireless BlackBerry -----Original Message----- From: joelle weaver Date: Tue, 8 Nov 2011 17:42:41 To: ; ; Subject: RE: [Histonet] micro wave processors >From my research I have found that the molecular water (polar) content and insulating fat content of the tissue types are added considerations. If you look at the physics and how microwaves penetrate and generate heat, this really helps in customizing schedules for different tissues and using its properties and effects to best advantage. The goods news is that the MW processors have more developed software that allows a wide manipulation ( in my experience) for processing protocols. Still, as you mentioned troubleshooting is as always required. Being newer technology, there are some implementation hurdles, but I have seen it work sucessfully for at least a large volume of various tissue types, signifigantly decreasing turn around times and leveling out workflow that allows staffing to be adjusted in favorable ways. Some labs I have worked at always seem to have certain tissues though that they have not been able to optimize a suitable protocol for processing with microwave assistance so far. Still in the refinement and development stages in many cases.Joelle Joelle Weaver MAOM, BA, (HTL) ASCP ? http://www.linkedin.com/in/joelleweaver ?> Date: Tue, 8 Nov 2011 09:30:13 -0800 > From: nelsonrnch@verizon.net > To: alvarez092703@hotmail.com > Subject: Re: [Histonet] micro wave processors > CC: Histonet@lists.utsouthwestern.edu > > You will always have a recommended protocol. It is up to us as HT/HTL? to determine what exactly will work. That is where TROUBLE SHOOTING falls in. > As I posted earlier, I use a certain type of microwave processor and because the recommended temps did not work on the type of tissue I was processing. I had to trouble shoot for what would work. > Again, my paraffin temps do not exceed 67C. > > THANK YOU, >? > PATTI RUBEN-NELSON? H.T.(ASCP) > PNP LABORATORY CONSULTANTS > SUPERVISOR/DGC > P.O. BOX 412 > CABAZON, CA. 92230 > cell (909) 841-9761 > nelsonrnch@verizon.net >? >? > CONFIDENTIALITY NOTICE: > This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants >? and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable > law.? Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful.? If you are not > the addressee, please promptly delete this message and notify the sender of > the delivery error by e-mail or you may call? 909-841-9761. > > > ________________________________ > From: jose and leticia alvarez > To: histonet@lists.utsouthwestern.edu > Sent: Tuesday, November 8, 2011 8:05 AM > Subject: [Histonet] micro wave processors > > > > > > I am in a mirotomy class, and we had a discussion about mircorwave processing.? We need an accurate temperature.? Freida states it should be at 84 degrees however we think she is incorrect.? please respond asap. thank you leticia alvarez????????????????????????? _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ?????????????????????????????????????? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Nov 8 16:40:04 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Nov 8 16:40:11 2011 Subject: [Histonet] release of tissues back to patient In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE473@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870E0FE473@dhmail.dhorg.org> Message-ID: <4EB96974.7400.0077.1@harthosp.org> In the state of Connecticut, the only agency licensed to handle human tissue or remains (other than hospitals) is a funeral home. Therefore, our policy is to only release tissue or remains to a funeral home after the patient or family has made the appropriate arrangements with them. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Hutton, Allison" 11/8/2011 12:57 PM >>> We had a question come up regarding giving patient's back their placentas (patient's request) after delivery. Our general rule is not to return tissues, except for religious reasons. We are now trying to come up with a concrete SOP for (or not) returning tissues. I was curious what other institutions are doing in regards to this topic. I know laws vary by location but I am looking for a general idea. Thank you in advance, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Tue Nov 8 18:20:13 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Nov 8 18:20:18 2011 Subject: [Histonet] Immune cells antibody Message-ID: Hi, You can detect NKT cells with CD57. They are also labelled with Granzyme B too, but I think CD57 will be a bit more specific. Amos On Tue, Nov 8, 2011 at 12:32 PM, wrote: > Message: 12 > Date: Mon, 7 Nov 2011 09:48:01 +0100 (CET) > From: "Carmen Maria Garcia Pascual" > Subject: [Histonet] Immune cells antibody > To: histonet@lists.utsouthwestern.edu > Message-ID: <2427631794carmaga6@uv.es> > Content-Type: text/plain; charset="ISO-8859-1" > > > Hi everyone: > > I want to detect immune cells (natural killers) in mouse's decidua, I > wonder if somebody knows a good antibody for that. > > thanks!!! > Carmen > From krns <@t> regionsjaelland.dk Wed Nov 9 04:29:04 2011 From: krns <@t> regionsjaelland.dk (Karen Inge Nielsen) Date: Wed Nov 9 04:29:13 2011 Subject: [Histonet] Peloris Rapid tissue processor Message-ID: Hi Muhammad At our lab we have a Peloris, and it's a great automated tissue processor. You'll get good results by the factory protocols but do also have the posibillity to create you own protocols for the tissue you are running most. That is what I do. I'm using the xylene substitute " Tissue Clear" and am running mostly fatty tissue. I'm almost using the factory time for ethanol but have extended the clearing and wax step a bit. I get really good results, and it's great to have this opportunity. A thing more I like about the Peloris is that you are not dependent of a specific company to sell you the reagents, and the Peloris is really easy to operate with. The ergonomics is also great. I look forward to get a Peloris more someday. Regards Karen Slagelse pathology Department Denmark From kcastillo <@t> frii.com Wed Nov 9 08:04:08 2011 From: kcastillo <@t> frii.com (kcastillo@frii.com) Date: Wed Nov 9 08:04:16 2011 Subject: [Histonet] PAS CONTROLS (BUGS) Message-ID: <17bfd8c50998a0ab61851d6b0e9563bf@frii.com> HI EVERYONE, WOULD LIKE TO KNOW WHERE I CAN FIND PAS (BUGS)CONTROL BLOCKS. DO NOT REALLY WANT TO BUY CONTROL SLIDES FROM A VENDOR. ANYONE OUT THERE HAVE ANY YOU COULD SEND ME LIKE ONE OR TWO? THANKS FOR ALL WHO ADDRESS. KRISTY From AHutton <@t> dh.org Wed Nov 9 08:15:00 2011 From: AHutton <@t> dh.org (Hutton, Allison) Date: Wed Nov 9 08:17:25 2011 Subject: [Histonet] release of tissues back to patient In-Reply-To: <4EB96974.7400.0077.1@harthosp.org> Message-ID: <38A56C4F4630D348A50B3720409270870E0FE476@dhmail.dhorg.org> Thank you to everyone who responded to my query. I have received enough information to put together both an SOP and a waiver form. Thanks Allison -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Tuesday, November 08, 2011 5:40 PM To: Hutton, Allison; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] release of tissues back to patient In the state of Connecticut, the only agency licensed to handle human tissue or remains (other than hospitals) is a funeral home. Therefore, our policy is to only release tissue or remains to a funeral home after the patient or family has made the appropriate arrangements with them. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Hutton, Allison" 11/8/2011 12:57 PM >>> We had a question come up regarding giving patient's back their placentas (patient's request) after delivery. Our general rule is not to return tissues, except for religious reasons. We are now trying to come up with a concrete SOP for (or not) returning tissues. I was curious what other institutions are doing in regards to this topic. I know laws vary by location but I am looking for a general idea. Thank you in advance, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From STACEY.LANGENBERG <@t> UCDENVER.EDU Wed Nov 9 09:47:20 2011 From: STACEY.LANGENBERG <@t> UCDENVER.EDU (Langenberg, Stacey) Date: Wed Nov 9 09:48:36 2011 Subject: [Histonet] PAS CONTROLS (BUGS) Message-ID: <87D0A6E5-6778-45F2-B77F-D64DE2D48D55@ucdenver.edu> PLEASE DELETE ME FROM GROUP. Thanks Stacey Sent from myTouch 4G ----- Reply message ----- From: "kcastillo@frii.com" To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] PAS CONTROLS (BUGS) Date: Wed, Nov 9, 2011 7:19 am HI EVERYONE, WOULD LIKE TO KNOW WHERE I CAN FIND PAS (BUGS)CONTROL BLOCKS. DO NOT REALLY WANT TO BUY CONTROL SLIDES FROM A VENDOR. ANYONE OUT THERE HAVE ANY YOU COULD SEND ME LIKE ONE OR TWO? THANKS FOR ALL WHO ADDRESS. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Wed Nov 9 11:06:13 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Nov 9 11:06:32 2011 Subject: [Histonet] Histo net In-Reply-To: <87D0A6E5-6778-45F2-B77F-D64DE2D48D55@ucdenver.edu> References: <87D0A6E5-6778-45F2-B77F-D64DE2D48D55@ucdenver.edu> Message-ID: <4940DF6D1C5FDF48931B6966AAEF93952AC66F@chimsx08.CHI.catholichealth.net> I'm not sure who the admin is here at histonet. Could the admin please contact me. Thanks William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Langenberg, Stacey Sent: Wednesday, November 09, 2011 9:47 AM To: kcastillo@frii.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PAS CONTROLS (BUGS) PLEASE DELETE ME FROM GROUP. Thanks Stacey Sent from myTouch 4G ----- Reply message ----- From: "kcastillo@frii.com" To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] PAS CONTROLS (BUGS) Date: Wed, Nov 9, 2011 7:19 am HI EVERYONE, WOULD LIKE TO KNOW WHERE I CAN FIND PAS (BUGS)CONTROL BLOCKS. DO NOT REALLY WANT TO BUY CONTROL SLIDES FROM A VENDOR. ANYONE OUT THERE HAVE ANY YOU COULD SEND ME LIKE ONE OR TWO? THANKS FOR ALL WHO ADDRESS. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lhbhcs1 <@t> comcast.net Wed Nov 9 11:23:52 2011 From: lhbhcs1 <@t> comcast.net (lhbhcs1@comcast.net) Date: Wed Nov 9 11:24:14 2011 Subject: [Histonet] Parts for Leica TP 1050 needed Message-ID: <940389800.1489747.1320859432629.JavaMail.root@sz0005a.emeryville.ca.mail.comcast.net> I am looking for parts for my processor. If you have a Leica TP 1050 that is no longer used can you contact me.? My 1050 has a problem and I need some parts for it.?? Thanks, LeRoy Brown HT(ASCP) HTL HCS, Inc. Everson, WA 98247 From KCross <@t> cvm.tamu.edu Wed Nov 9 12:05:12 2011 From: KCross <@t> cvm.tamu.edu (Cross, Kelly) Date: Wed Nov 9 12:05:16 2011 Subject: [Histonet] unsubscribe Message-ID: <869848DDBB7C5D4896A569A38B814E69055253E5@CVMMB01.cvm.tamu.edu> Kelly Cross B.S., HT (ASCP) Medical Laboratory Supervisor Texas A&M University VTPB Histology Lab VMA bldg. 214 College Station, TX 77843-4467 Office: 979-862-3658 Lab: 979-845-5149 From Erik.Dokken <@t> onassignment.com Wed Nov 9 13:42:24 2011 From: Erik.Dokken <@t> onassignment.com (Erik Dokken) Date: Wed Nov 9 13:42:34 2011 Subject: [Histonet] Full time HistoTech Opportunity in Houston, TX In-Reply-To: <201110311600.p9VFui6o003119@smtp12.onasgn.net> References: <201110311600.p9VFui6o003119@smtp12.onasgn.net> Message-ID: On Assignment Healthcare has a full-time opportunity with a lab in the West Chase Area of Houston. If you or someone you know would like to hear more about this opportunity please email us today! Erik Dokken Area Manager - No. CA, WA, and TX On Assignment, Inc. Local Allied Healthcare NASDAQ: ASGN www.oahealthcare.com People First -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, October 31, 2011 9:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 95, Issue 37 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. For zebrafish larvae section (=?gb2312?B?tNTk7LfJ?=) 2. Re: Stripping antibodies (Claire Weston) 3. Re: Stripping antibodies (John Kiernan) 4. RE: SALARY (Heath, Nancy L.) 5. RE: SALARY (Heath, Nancy L.) 6. RE: SALARY (Heath, Nancy L.) 7. Must be Monday... (Breeden, Sara) 8. RE: Must be Monday... (Blazek, Linda) 9. Florida Medical Directorship (Hale, Meredith) 10. Problems with Frozen tissues (Igor Deyneko) 11. Stripping antibodies (C.M. van der Loos) 12. RE: Must be Monday... (Beckham, Sharon) 13. RE: SALARY (Mayer,Toysha N) 14. RE: SALARY (joelle weaver) 15. RE: need help with TMA (Helen Fedor) 16. RE: RE: SALARY (joelle weaver) 17. background checks required by AHCA (Nicole Tatum) 18. Dako Pr on Ventana? (Orr, Rebecca) ---------------------------------------------------------------------- Message: 1 Date: Mon, 31 Oct 2011 02:21:03 +0800 From: =?gb2312?B?tNTk7LfJ?= Subject: [Histonet] For zebrafish larvae section To: Message-ID: Content-Type: text/plain; charset="gb2312" Hi, My respects! I wanted to do some zebrafish larvae sectioning. But some slice I made showed that the tissue inside had been broken. As to me, I thought the problem might be in dehydration time and and the razor I used. So can you please give me some advice to improve the process? In addition, I wanted to make some cross section. But it was difficult to me to control the orientation of the larvae. Thanks a lot! Best,Joseph Cong ------------------------------ Message: 2 Date: Sun, 30 Oct 2011 11:58:19 -0700 From: "Claire Weston" Subject: Re: [Histonet] Stripping antibodies To: "John Kiernan" Cc: histonet@lists.utsouthwestern.edu, amosbrooks@gmail.com Message-ID: <20111030115819.1ECE91E5@resin12.mta.everyone.net> Content-Type: text/plain; charset="UTF-8"
Thank you everyone for your advice, I really appreciate it!  I am doing a multiplex immunofluorescent assay (4 channels) and I am planning to strip the antibodies and reprobe with a second round of antibodies.  The antibodies are labelled with quantum dots so I am not sure how that will influence things, but luckily I won't have the DAB problem several of you mentioned.  I think I will try several of these techniques and evaluate which works the best.
 
Thanks again for all your help,
 
Claire 

--- jkiernan@uwo.ca wrote:

From: John Kiernan <jkiernan@uwo.ca>
To: histonet@lists.utsouthwestern.edu, cweston@valasciences.com
Cc: Amos Brooks <amosbrooks@gmail.com>
Subject: Re: [Histonet] Stripping antibodies
Date: Sun, 30 Oct 2011 12:09:58 -0400

 
Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow.  If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents.
 
Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want?  This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody.
 
Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars).  The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios,  1999, is very good.
 
John Kiernan
Anatomy, UWO 
London, Canada 
= = = =
On 29/10/11, Amos Brooks <amosbrooks@gmail.com> wrote:
Hi,
    Stripping sites are usually found in the more seedy areas of large
cities ... Oh wait you meant ... nevermind :-) If you developed the reaction
with DAB, you may be in a difficult position. DAB is a really strong
reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in
5% potassium premanganate for 5 min followed by 5% oxalic acid until it is
clear (usually a couple of minutes). Unfortunately, I am not sure what
effect this will have on the epitopes you are looking for, but this is
usually what is used to clean precipitated DAB from instruments it should
work on the tissue too.
    If you use another chromogen such as AEC it is much easier (to many
people's dismay) to remove this end product. Using alkaline phosphatase will
be easy to remove the chromogens as well. It would also be very easy to do
this with fluorescent tags too. Just remove the coverslip and dip the slide
in ethanol and it will remove the colored end product. Then just start over
for the new antigen.

Good luck,
Amos

On Fri, Oct 28, 2011 at 11:50 AM, <histonet-request@lists.utsouthwestern.edu
> wrote:

> Message: 2
> Date: Thu, 27 Oct 2011 10:30:40 -0700
> From: "Claire Weston" <cweston@valasciences.com>
> Subject: [Histonet] Stripping antibodies
> To: <histonet@lists.utsouthwestern.edu>
> Message-ID: <002401cc94ce$2619ca60$724d5f20$@com>
> Content-Type: text/plain;       charset="us-ascii"
>
> Hi!
>
>
>
> Does anyone have experience in IHC stripping antibodies from a tissue
> section and re-probing with different antibodies?  What is the best way to
> do that?  If you could share your stripping protocol or experience I would
> appreciate it - thanks!
>
>
>
> Claire
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 
 
On 29/10/11, Amos Brooks <amosbrooks@gmail.com> wrote:
Hi,
    Stripping sites are usually found in the more seedy areas of large
cities ... Oh wait you meant ... nevermind :-) If you developed the reaction
with DAB, you may be in a difficult position. DAB is a really strong
reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in
5% potassium premanganate for 5 min followed by 5% oxalic acid until it is
clear (usually a couple of minutes). Unfortunately, I am not sure what
effect this will have on the epitopes you are looking for, but this is
usually what is used to clean precipitated DAB from instruments it should
work on the tissue too.
    If you use another chromogen such as AEC it is much easier (to many
people's dismay) to remove this end product. Using alkaline phosphatase will
be easy to remove the chromogens as well. It would also be very easy to do
this with fluorescent tags too. Just remove the coverslip and dip the slide
in ethanol and it will remove the colored end product. Then just start over
for the new antigen.

Good luck,
Amos

On Fri, Oct 28, 2011 at 11:50 AM, <histonet-request@lists.utsouthwestern.edu
> wrote:

> Message: 2
> Date: Thu, 27 Oct 2011 10:30:40 -0700
> From: "Claire Weston" <cweston@valasciences.com>
> Subject: [Histonet] Stripping antibodies
> To: <histonet@lists.utsouthwestern.edu>
> Message-ID: <002401cc94ce$2619ca60$724d5f20$@com>
> Content-Type: text/plain;       charset="us-ascii"
>
> Hi!
>
>
>
> Does anyone have experience in IHC stripping antibodies from a tissue
> section and re-probing with different antibodies?  What is the best way to
> do that?  If you could share your stripping protocol or experience I would
> appreciate it - thanks!
>
>
>
> Claire
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
------------------------------ Message: 3 Date: Mon, 31 Oct 2011 02:12:52 -0400 From: John Kiernan Subject: Re: [Histonet] Stripping antibodies To: cweston@valasciences.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <7690e6ad626d2.4eae0424@uwo.ca> Content-Type: text/plain; CHARSET=US-ASCII With quantum dots and a modern fluorescence microscope with "channels" it should be possible to label 2 or 3 antigens simultaneously, if you have all the right primaries and labelled secondaries. The company that sells you the quantum dot labelled secondaries or other amplification reagents should be in a position to tell you exactly what to do. Do they refund your hard-earned grant money if their expensive product fails to perform as advertized? Immunohistochemistry with non-fluorescent colours (available in brown, black, red and various blues) has advantages: (a) You do not need a fluorescence microscope. Modern fluorescence microscopes are very expensive; the old ones from the 1960s are no longer be good enough to provide publication-quality pictures. (b) Immunostained slides can be kept for many years in boxes at room temperature. (c) The colours do not fade in permanently mounted preparations. (c) You are not bound to use a commercially sold kit, which may not be optimized for your research. If you go by the book (= any book with references that you can follow up), you will do your multiple immunostains intelligently. Never follow a set of instructions without knowing or questioning the reason for ever step. John Kiernan Anatomy, UWO London, Canada. = = = On 30/10/11, Claire Weston wrote: > > > > Thank you everyone for your advice, I really appreciate it! I am doing a multiplex immunofluorescent assay (4 channels) and I am planning to strip the antibodies and reprobe with a second round of antibodies. The antibodies are labelled with quantum dots so I am not sure how that will influence things, but luckily I won't have the DAB problem several of you mentioned. I think I will try several of these techniques and evaluate which works the best. > > Thanks again for all your help, > > Claire > > --- jkiernan@uwo.ca wrote: > > From: John Kiernan > To: histonet@lists.utsouthwestern.edu, cweston@valasciences.com > Cc: Amos Brooks > Subject: Re: [Histonet] Stripping antibodies > Date: Sun, 30 Oct 2011 12:09:58 -0400 > > > > Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow. If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents. > > Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want? This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody. > > Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars). The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios, 1999, is very good. > > John Kiernan > Anatomy, UWO > London, Canada > = = = = > On 29/10/11, Amos Brooks wrote: > > > > > Hi, > > Stripping sites are usually found in the more seedy areas of large > > cities ... Oh wait you meant ... nevermind :-) If you developed the reaction > > with DAB, you may be in a difficult position. DAB is a really strong > > reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in > > 5% potassium premanganate for 5 min followed by 5% oxalic acid until it is > > clear (usually a couple of minutes). Unfortunately, I am not sure what > > effect this will have on the epitopes you are looking for, but this is > > usually what is used to clean precipitated DAB from instruments it should > > work on the tissue too. > > If you use another chromogen such as AEC it is much easier (to many > > people's dismay) to remove this end product. Using alkaline phosphatase will > > be easy to remove the chromogens as well. It would also be very easy to do > > this with fluorescent tags too. Just remove the coverslip and dip the slide > > in ethanol and it will remove the colored end product. Then just start over > > for the new antigen. > > > > Good luck, > > Amos > > > > On Fri, Oct 28, 2011 at 11:50 AM, > > wrote: > > > > > Message: 2 > > > Date: Thu, 27 Oct 2011 10:30:40 -0700 > > > From: "Claire Weston" > > > Subject: [Histonet] Stripping antibodies > > > To: > > > Message-ID: <002401cc94ce$2619ca60$724d5f20$@com> > > > Content-Type: text/plain; charset="us-ascii" > > > > > > Hi! > > > > > > > > > > > > Does anyone have experience in IHC stripping antibodies from a tissue > > > section and re-probing with different antibodies? What is the best way to > > > do that? If you could share your stripping protocol or experience I would > > > appreciate it - thanks! > > > > > > > > > > > > Claire > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > On 29/10/11, Amos Brooks wrote: > > > > > Hi, > > Stripping sites are usually found in the more seedy areas of large > > cities ... Oh wait you meant ... nevermind :-) If you developed the reaction > > with DAB, you may be in a difficult position. DAB is a really strong > > reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in > > 5% potassium premanganate for 5 min followed by 5% oxalic acid until it is > > clear (usually a couple of minutes). Unfortunately, I am not sure what > > effect this will have on the epitopes you are looking for, but this is > > usually what is used to clean precipitated DAB from instruments it should > > work on the tissue too. > > If you use another chromogen such as AEC it is much easier (to many > > people's dismay) to remove this end product. Using alkaline phosphatase will > > be easy to remove the chromogens as well. It would also be very easy to do > > this with fluorescent tags too. Just remove the coverslip and dip the slide > > in ethanol and it will remove the colored end product. Then just start over > > for the new antigen. > > > > Good luck, > > Amos > > > > On Fri, Oct 28, 2011 at 11:50 AM, > > wrote: > > > > > Message: 2 > > > Date: Thu, 27 Oct 2011 10:30:40 -0700 > > > From: "Claire Weston" > > > Subject: [Histonet] Stripping antibodies > > > To: > > > Message-ID: <002401cc94ce$2619ca60$724d5f20$@com> > > > Content-Type: text/plain; charset="us-ascii" > > > > > > Hi! > > > > > > > > > > > > Does anyone have experience in IHC stripping antibodies from a tissue > > > section and re-probing with different antibodies? What is the best way to > > > do that? If you could share your stripping protocol or experience I would > > > appreciate it - thanks! > > > > > > > > > > > > Claire > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > ------------------------------ Message: 4 Date: Mon, 31 Oct 2011 06:20:57 -0400 From: "Heath, Nancy L." Subject: RE: [Histonet] SALARY To: "Richard Cartun" , , , "Caroline Pratt" Message-ID: <130E8991F210424096EFC6F42EA33B240843564F@LSCOEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="us-ascii" "Histotechnologists are the most valuable employees in the laboratory today!" THANK YOU DR. CARTUN!! Nancy Heath, HT (ASCP) Neuropathology Technician Neuropathology Department Rhode Island Hospital President - Rhode Island Society for Histotechnology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 28, 2011 12:52 PM To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Caroline Pratt Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 31 Oct 2011 06:29:12 -0400 From: "Heath, Nancy L." Subject: RE: [Histonet] SALARY To: "John Shelley" , "Pratt, Caroline" , "Richard Cartun" , , Message-ID: <130E8991F210424096EFC6F42EA33B2408435651@LSCOEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Just got offered a lead tech position, salary of $85600.00 in a private lab opened by a group of docs. Didn't take it because the health insurance plan they offered was lousey. 75% health covered with a 250.00 deductable, dental covered at 60% and no coverage for family or husband....would of had to pay that out of pocket. I'm in New England. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Friday, October 28, 2011 2:25 PM To: Pratt, Caroline; Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY With all that said what are your going rates in this area that you are speaking. Kind Regards! ? John J Shelley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Friday, October 28, 2011 2:19 PM To: Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SALARY If that is an option, I would agree, but non-profit teaching hospitals have compensation ranges and grids according to education and experience. We keep every employee with the same education and skill set at the same compensation for an equitable pay system. If we feel our employees are being under paid we will conduct a market analysis and the rates will be increased for all applicable employees if the market analysis justifies it. Car :) -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Friday, October 28, 2011 12:52 PM To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, Caroline Subject: RE: [Histonet] SALARY I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------------------------------------------------ - This message was secured by ZixCorp(R). The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 31 Oct 2011 06:30:04 -0400 From: "Heath, Nancy L." Subject: RE: [Histonet] SALARY To: "Breeden, Sara" , "Podawiltz, Thomas" , "Richard Cartun" , , , "Caroline Pratt" Message-ID: <130E8991F210424096EFC6F42EA33B2408435652@LSCOEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="us-ascii" I agree :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, October 28, 2011 3:22 PM To: Podawiltz, Thomas; Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Caroline Pratt Subject: RE: [Histonet] SALARY I nominate Dr. Cartun as the Patron Saint for Histopathology. We should work on a statue and a Mission Statement for him... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 31 Oct 2011 06:33:01 -0600 From: "Breeden, Sara" Subject: [Histonet] Must be Monday... To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DFB81@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ------------------------------ Message: 8 Date: Mon, 31 Oct 2011 08:40:02 -0400 From: "Blazek, Linda" Subject: [Histonet] RE: Must be Monday... To: "'Breeden, Sara'" , "Histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E39127401B441@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" That's a hoot Sally! I don't think that DST of old was even this past weekend. For the past 3 years my processor changed on the DST of old and the powers that be told me it was impossible because that wasn't programmed into my processor's data. This year I forgot about it and it didn't change on the old date. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 8:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 31 Oct 2011 08:01:31 -0500 From: "Hale, Meredith" Subject: [Histonet] Florida Medical Directorship To: Message-ID: <6F33D8418806044682A391273399860F0A5B2974@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" What are the true regulations' in Florida as far as time required for a Medical Director to be on site at the lab ? I am finding conflicting information and would like to know what is truly required. Thanks Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 ------------------------------ Message: 10 Date: Mon, 31 Oct 2011 09:56:10 -0400 From: Igor Deyneko Subject: [Histonet] Problems with Frozen tissues To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I'm looking for some advice on frozen tissues. This is the first time I'm doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut well onto special Gold Plus slides from Fisher. Then, when I was ready to stain the slides, i air dried them fro an hour and wanted to do H&E and Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on preventing this mischief? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ------------------------------ Message: 11 Date: Mon, 31 Oct 2011 14:02:04 +0000 From: "C.M. van der Loos" Subject: [Histonet] Stripping antibodies To: "histonet@lists.utsouthwestern.edu" Cc: "'amosbrooks@gmail.com'" Message-ID: Content-Type: text/plain; charset="us-ascii" Amos and John, I do not agree with John saying that "antibodies could be easily removed by acidity pH1-2". High affinity primaries will stay at the tissue section nicely bound to the epitope. Try for example SMA, clone 1A4 antibody in the usual dilution. After this incubation strip off the antibody by glycin-HCl buffer pH2 and then apply an anti-mouse detection system. You will stain SMA for sure. The only way to get antibodies off is 10 min boiling with citrate pH6.0 or so, as in HIER. I have described that in JOH in 2008, vol 31, pp. 119-127, being the basis of an AP sequential double staining. Chris van der Loos Academic Medical Center Dept. of Pathology M2-230 Amsterdam The Netherlands Date: Sun, 30 Oct 2011 12:09:58 -0400 From: John Kiernan Subject: Re: [Histonet] Stripping antibodies To: histonet@lists.utsouthwestern.edu, cweston@valasciences.com Cc: Amos Brooks Message-ID: <7640acec48487.4ead3e96@uwo.ca> Content-Type: text/plain; CHARSET=US-ASCII Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow. If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents. Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want? This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody. Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars). The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios, 1999, is very good. John Kiernan Anatomy, UWO London, Canada ------------------------------ Message: 12 Date: Mon, 31 Oct 2011 09:22:38 -0500 From: "Beckham, Sharon" Subject: [Histonet] RE: Must be Monday... To: "'Blazek, Linda'" , "'Breeden, Sara'" , "Histonet@lists.utsouthwestern.edu" Message-ID: <2C40E43D1F7A56408C4463FD245DDDF98AEE2C42@EXCHMB-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" Yes it was this past weekend back when. My alarm clock went to DST Sunday morning. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, October 31, 2011 7:40 AM To: 'Breeden, Sara'; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Must be Monday... That's a hoot Sally! I don't think that DST of old was even this past weekend. For the past 3 years my processor changed on the DST of old and the powers that be told me it was impossible because that wasn't programmed into my processor's data. This year I forgot about it and it didn't change on the old date. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, October 31, 2011 8:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Must be Monday... Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 31 Oct 2011 09:25:58 -0500 From: "Mayer,Toysha N" Subject: [Histonet] RE: SALARY To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Add location to that. $28-32 is great for places that do not have an abundant supply of techs. Here in Houston, we have 2 schools and a very large medical center. Entry level techs are not hard to find. Graduates expect $23-28 minimum depending on facility. In other states, neighboring Louisiana and such, they expect $21-24. It just depends on the cost of living in the area. Entry supervisors earn $30+ an hour, but again, it is based on cost of living and work conditions. Reference labs pay more, but expect more. My first supervisor job paid $32, and I worked like crazy. It wasn't worth it. You get what you pay for though, and in this economic climate, the high salaries are not as plentiful as they once were. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Message: 6 Date: Fri, 28 Oct 2011 12:52:23 -0400 From: "Richard Cartun" Subject: RE: [Histonet] SALARY To: ,, "Caroline Pratt" Message-ID: <4EAAA586.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> I would say $28 to $32 dollars an hour depending on experience and education. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com Sent: Friday, October 28, 2011 7:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] SALARY HI EVERYONE, WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 95, Issue 34 **************************************** ------------------------------ Message: 14 Date: Mon, 31 Oct 2011 14:35:21 +0000 From: joelle weaver Subject: RE: [Histonet] SALARY To: , , , , , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I know the cost of living is greater on the east coast, but that sounds good to me. The insurance is not better, and as an HTL I make less than 1/2 of that! Things are determined by the market I realize, but all these postings are sure making me feel bad and also realize that I am not marketing myself well.... Joelle Weaver MAOM, BA, (HTL) ASCP > Date: Mon, 31 Oct 2011 06:29:12 -0400 > From: NHeath@Lifespan.org > To: jshelley@sanfordburnham.org; Caroline.Pratt@uphs.upenn.edu; Rcartun@harthosp.org; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] SALARY > CC: > > Just got offered a lead tech position, salary of $85600.00 in a private lab opened by a group of docs. Didn't take it because the health insurance plan they offered was lousey. 75% health covered with a 250.00 deductable, dental covered at 60% and no coverage for family or husband....would of had to pay that out of pocket. > I'm in New England. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley > Sent: Friday, October 28, 2011 2:25 PM > To: Pratt, Caroline; Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] SALARY > > With all that said what are your going rates in this area that you are speaking. > > Kind Regards! > > John J Shelley > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline > Sent: Friday, October 28, 2011 2:19 PM > To: Richard Cartun; kcastillo@frii.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] SALARY > > If that is an option, I would agree, but non-profit teaching hospitals > have compensation ranges and grids according to education and > experience. We keep every employee with the same education and skill > set at the same compensation for an equitable pay system. If we feel > our employees are being under paid we will conduct a market analysis and > the rates will be increased for all applicable employees if the market > analysis justifies it. > > Car :) > > -----Original Message----- > From: Richard Cartun [mailto:Rcartun@harthosp.org] > Sent: Friday, October 28, 2011 12:52 PM > To: kcastillo@frii.com; Histonet@lists.utsouthwestern.edu; Pratt, > Caroline > Subject: RE: [Histonet] SALARY > > I think that's low. If you find a good candidate with years of > experience I would pay them whatever it takes to get them in the door. > It's like "Free Agency" in baseball; if you want a good player, you need > to put the "money on table". Histotechnologists are the most valuable > employees in the laboratory today! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> "Pratt, Caroline" 10/28/2011 9:21 AM > >>> > I would say $28 to $32 dollars an hour depending on experience and > education. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > kcastillo@frii.com > Sent: Friday, October 28, 2011 7:54 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] SALARY > > HI EVERYONE, > > WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID > THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM > LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this e-mail message is intended only for > the personal and confidential use of the recipient(s) named above. If > the reader of this message is not the intended recipient or an agent > responsible for delivering it to the intended recipient, you are hereby > notified that you have received this document in error and that any > review, dissemination, distribution, or copying of this message is > strictly prohibited. If you have received this communication in error, > please notify us immediately by e-mail, and delete the original message. > > > > > > ------------------------------------------------------------------------ > - > This message was secured by ZixCorp(R). > > > The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Mon, 31 Oct 2011 14:36:26 +0000 From: Helen Fedor Subject: [Histonet] RE: need help with TMA To: 'Patricia F Lott' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello, Do 30 minutes at 37 degrees on a glass slide. take from oven and apply gentle pressure to center of block and slide complex, cool to RT and repeat 1 or two times. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia F Lott Sent: Friday, October 28, 2011 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] need help with TMA We have recently started doing tissue microarrays. The problem we have is that some of the small cores drop out after the first few sections. I think my problem is in the step where we are supposed to melt the donor and recipient blocks together. We tried 5 minutes at 37 degrees, and then minutes at 60 degrees x3, with cooling to room temp in between. Any suggestions? Thanks, Patty Lott, UAB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Mon, 31 Oct 2011 14:40:13 +0000 From: joelle weaver Subject: RE: [Histonet] RE: SALARY To: , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Still sounds good to me, at my first supervisor's job ( the credentials I had at the time were HTL + Bachelor's), and I made less than $23 per hour and worked an average of 16 hours a day with no OT or comp time that was ever granted ( though promised). I guess I am just trying to say, count your blessings. Having a school near by really seems to impact the supply and demand and the general hiring environment. What I have noticed also is that when you go somewhere and the cost of living is less the tax burden is greater, so it has always seemed to come "out in the wash". Anyhow, all these salary revelations have really made me think, thanks for the postings. Joelle Weaver MAOM, BA, (HTL) ASCP > From: TNMayer@mdanderson.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 31 Oct 2011 09:25:58 -0500 > Subject: [Histonet] RE: SALARY > > Add location to that. $28-32 is great for places that do not have an abundant supply of techs. Here in Houston, we have 2 schools and a very large medical center. Entry level techs are not hard to find. Graduates expect $23-28 minimum depending on facility. > In other states, neighboring Louisiana and such, they expect $21-24. It just depends on the cost of living in the area. > Entry supervisors earn $30+ an hour, but again, it is based on cost of living and work conditions. Reference labs pay more, but expect more. > > My first supervisor job paid $32, and I worked like crazy. It wasn't worth it. > You get what you pay for though, and in this economic climate, the high salaries are not as plentiful as they once were. > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > Message: 6 > Date: Fri, 28 Oct 2011 12:52:23 -0400 > From: "Richard Cartun" > Subject: RE: [Histonet] SALARY > To: ,, > "Caroline Pratt" > Message-ID: <4EAAA586.7400.0077.1@harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> "Pratt, Caroline" 10/28/2011 9:21 AM >>> > I would say $28 to $32 dollars an hour depending on experience and education. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kcastillo@frii.com > Sent: Friday, October 28, 2011 7:54 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] SALARY > > HI EVERYONE, > > WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID > THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM > LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 95, Issue 34 > **************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Mon, 31 Oct 2011 10:33:12 -0400 (EDT) From: "Nicole Tatum" Subject: [Histonet] background checks required by AHCA To: histonet@lists.utsouthwestern.edu Message-ID: <1533.208.62.167.196.1320071592.squirrel@webmail.realpages.com> Content-Type: text/plain;charset=iso-8859-1 I am currently filing out my ahca renewal application. There are many changes and the form and I do not find it user friendly. Anyways here my question: Medical directors and chief finicial officers or any person who stands to gain profit must undergo a level 2 background screeening. On the new app is states all empolyees and health care providers. I called acha licensing division and they said only directors and so forth. But, on ahca website statue 408(something, ill have to get exact number) when into effect in 2010 that says all employees and newly hired employees must underground background screening. So does all the arnp, pa, ht, and ma's need to be screened because we have a lab? Nicole Tatum, HT ASCP http://ahca.myflorida.com/mchq/long_term_care/Background_Screening/BGS_WhoRequiredToBeScreened.pdf taken directly from ahca site: Employees and Contractors employed before August 1, 2010 Every employee/contractor must attest to meeting the requirements of this chapter and agreeing to inform the employer immediately if arrested for any of the disqualifying offenses while employed by the employer. [Section 435.05(2)]. This attestation must be maintained in the employee?s personnel file. You may use the Affidavit of Compliance with Background Screening to satisfy the attestation requirement. If an employer becomes aware that an employee/contractor has been arrested for a disqualifying offense, the employer must remove the employee/contractor from contact with any vulnerable person that places the employee/contractor in a role that requires background screening until the arrest is resolved in a way that the employer determines that the employee/contractor is still eligible for employment/contracting under this chapter. [Section 435.06(2)(b)] Rescreening ------------------------------ Message: 18 Date: Mon, 31 Oct 2011 10:56:17 -0500 From: "Orr, Rebecca" Subject: [Histonet] Dako Pr on Ventana? To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Friends, If anyone is using Dako PR concentrate clone 1294 on a Ventana, could you please contact me separately? I'd like some advice. Thank you Becky Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 -----Original Message----- Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 95, Issue 37 **************************************** From jcox90 <@t> yahoo.com Wed Nov 9 16:17:41 2011 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Wed Nov 9 16:17:44 2011 Subject: [Histonet] Cassette Baskets for VIP E series Message-ID: <1320877061.36679.YahooMailNeo@web161602.mail.bf1.yahoo.com> Hi Netters, Does anyone have a couple of extra VIP E series?cassette baskets they would like to sell? Please contact me at this email address. Thanks in advance!! Have a wonderful day. Jill Cox, HT ASCP From Sandra.Harrison3 <@t> va.gov Thu Nov 10 11:46:01 2011 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Thu Nov 10 11:46:12 2011 Subject: [Histonet] Chicago Sky Blue stain vs. KOH - striking comparison Message-ID: Does anyone have ordering information for a stain called Chicago Sky Blue(CSB)? I just saw an article in the NOV issue of ASCP LABMEDICINE (page 649) comparing KOH vs. CSB and, wow, is that a beautiful stain. The results were dramatic, with about a 25-50% increase in the Dermatophytes identified and about a 5X increase in the Pityriasis versicolor identification. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 From wbenton <@t> cua.md Thu Nov 10 11:56:10 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Nov 10 11:59:32 2011 Subject: [Histonet] RE: Chicago Sky Blue stain vs. KOH - striking comparison In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD9206C204435@CUAEXH1.GCU-MD.local> Here is what I found via a google search http://www.sigmaaldrich.com/catalog/ProductDetail.do?D7=0&N5=SEARCH_CONCAT_PNO%7CBRAND_KEY&N4=C8679%7CSIGMA&N25=0&QS=ON&F=SPEC http://www.delasco.com/pcat/pdf/ChicagoBlue.pdf Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. [Sandra.Harrison3@va.gov] Sent: Thursday, November 10, 2011 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chicago Sky Blue stain vs. KOH - striking comparison Does anyone have ordering information for a stain called Chicago Sky Blue(CSB)? I just saw an article in the NOV issue of ASCP LABMEDICINE (page 649) comparing KOH vs. CSB and, wow, is that a beautiful stain. The results were dramatic, with about a 25-50% increase in the Dermatophytes identified and about a 5X increase in the Pityriasis versicolor identification. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From melissa <@t> alliedsearchpartners.com Thu Nov 10 15:12:55 2011 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Thu Nov 10 15:13:03 2011 Subject: [Histonet] Talented Histotech Needed in SC (Near Athens, GA and Asheville, NC) Message-ID: MPath Search Partners, a division of Allied Search Partners is currently looking for a Full Time/Permanent Histotech in Anderson, SC. The lab is just 68 miles Northeast of Athens, GA & 90 miles South of Asheville, NC. Position Title: Histotechnologist/Histotechnician-This position is a Permanent position. We are looking for a long term Histotech. Come join a well team oriented relaxing environment! The lab offers cutting edge technologies and top-notch equipment. We are looking for a talented histotech to join a fantastic team! Shift: Monday-Friday Full Time Permanent Location & Environment: Private Pathology Laboratory Anderson, SC area Requirements HT or HTL ASCP preferred Grossing experience a plus MOHS experience a plus Summary of Duties Cutting, Embedding, Grossing, Staining etc. To apply: Please send information to melissa@alliedsearchpartners.com 1. Resume 2. Expected Salary Thank you! -- Melissa Phelan, President Laboratory Staffing Allied Search Partners http://www.linkedin.com/in/melissaphelan P: 888-388-7571 F: 888-388-7572 C: 407-697-1175 www.alliedsearchpartners.com From daniela.bodemer <@t> mcri.edu.au Thu Nov 10 16:28:07 2011 From: daniela.bodemer <@t> mcri.edu.au (Daniela Bodemer) Date: Thu Nov 10 16:28:12 2011 Subject: FW: [Histonet] Chicago Sky Blue stain vs. KOH - striking comparison Message-ID: <9DF797D618351549B984596F01A1FE1D01FCD6F7@murmx.mcri.edu.au> We use Chicago Sky Blue 6B from Sigma C-8679 (2610-05-1). We make our solution as followed: 1g CSB 6B 1ml DiMethylsulphoxid 99ml Dist water Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03 9345 4116) E daniela.bodemer@mcri.edu.au www.mcri.edu.au -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Friday, 11 November 2011 4:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chicago Sky Blue stain vs. KOH - striking comparison Does anyone have ordering information for a stain called Chicago Sky Blue(CSB)? I just saw an article in the NOV issue of ASCP LABMEDICINE (page 649) comparing KOH vs. CSB and, wow, is that a beautiful stain. The results were dramatic, with about a 25-50% increase in the Dermatophytes identified and about a 5X increase in the Pityriasis versicolor identification. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email If you have any question, please contact MCRI IT Helpdesk for further assistance. ______________________________________________________________________ ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From LStadler <@t> cbiolabs.com Fri Nov 11 08:50:41 2011 From: LStadler <@t> cbiolabs.com (Lyn Stadler) Date: Fri Nov 11 08:50:46 2011 Subject: [Histonet] Exam Prep Info/Guidance/Suggestions Message-ID: <98CC14B915EBA84B9A326D45CC3C1DEC3AB8FF5E@cbiolabs05.CBiolabs.local> All ~ I am about to begin preparing for the HTL certification exam. (I supposse it is only fair to mention that this is will be my second attempt. My first exam taken last month with a score of 382 out of a minimum passing score of 400). As a "research" Histology Technician, a good majority of the material covered on the exam was new to me. My prep for the first exam was to read "Histotechnology, A Self-Instructional Text, 3rd Edtion" by Carson and Hladik and then answer a lot of "practice questions" from the Board of Registry Second Edition Practice Questions book. I focused mostly on the special staining info and images because of the high concentration of questions on the exam as well as my limited professionnal exposure to these...unfortunately, it was still the area i did most poorly in on the exam. In order to go a little more in depth the second time around, my plan is to is to re-read each chapter ( of Carson), then assess my knowledge by being sure I can do each of the "Chapter Objectives" and then answer the questions in the self-assessement workbook, then answer the questions on that particular chapter topic from the Seond Edition of the Board of Registry Study Guide, Practice Questions for the Histotechnology Exams (The Purple Book). I have other textbooks at my disposal, but as a mostly visual learner, I find Carson's approach to best get the information into my brain! Again, once I feel comfortable with all the basic knowledge, I would focus mostly on staining. Specifically, I would like feedback from anyone who has recently passed the exam about my approach, and suggestions for other ideas. Also, I have very limited CAP/Joint Comission type knowlege and wonder if anyone can offer a resource for "basics" for someone like me in research who is not presented with the info and regulations on a daily basis! Also, any suggestions for images online of special stains would be a great resource for me. Thanks in advance for any and all info! Lyn This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From joelleweaver <@t> hotmail.com Fri Nov 11 09:24:44 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Nov 11 09:24:51 2011 Subject: [Histonet] Exam Prep Info/Guidance/Suggestions In-Reply-To: <98CC14B915EBA84B9A326D45CC3C1DEC3AB8FF5E@cbiolabs05.CBiolabs.local> References: <98CC14B915EBA84B9A326D45CC3C1DEC3AB8FF5E@cbiolabs05.CBiolabs.local> Message-ID: LynI compliment you on your organized approach. I did not take the exam "recently", I am old now! But as part of my previous job I spent a lot of time analyzing the content and exam topics for the HT exam. If you look at both outlines (HT and HTL) you will see that they cover a broad scope of histotechnology. Many people assume that it is mostly "routine" histology, but in fact they throw some obscure things in there and some histology speciality practices, and my understanding from a teleconference on the topic from the exam preparers and working with ASCP and NAACLS, is that they want to encompass the full spectrum of possible environments where histologists may work.There are key differences between clinical and research environments, but the basic histology theory is the same, just practices and emphasis differences. This makes sense if you think about their purpose(s). So if you understand the fundamentals you should be able to move easily between the environments and I believe that is what they are going for.Anyhow, I think that the newest Carson edition is good, but my personal feeling is that it is quite heavy on the very routine and most common special stains only, and though it is much better than the previous ones ( which I felt were very superficial), it is still mostly routine since it is meant to be an introductory text. I recommend the Sheehan and Hrapchak text for studying the less common special stains, if you can utilize this for the underlying chemistry and theory. Though bear in mind that this text is quite old now and some techniques presented are pretty outdated, but you can get the theory from it. It is not as "reader" friendly, and less pictures, mostly diagrams if any, but the chemistry is laid out pretty well in my opinion, though you have to dig around a bit. My advice to people has always been to make some attempt to understand the underlying chemistry so that you are not trying to memorize stains. They can be grouped according to chemical interaction and target tissue element for study purposes. Then you will not be thrown off by "modifications" and variations so much for histochemical stains. I organized it this way when I taught chemistry of stains and this seems to help people digest the information without getting overwhelmed by all the stains out there. The web is good these days for providing practice images and gets you used to seeming them on a monitor- though there is no replacement for spending time at the microscope. I think being strong in tissue identification also helps immensly for both HC and IHC staining, so any time you spend with that at the scope is great in my opinion. The BOR study guide is good to get you used to the wording and presentation of the questions that are used ( being retired or not used exam questions), and gives you some idea of the scope of the content. The more difficult questions are marked with an * in the guide, and they represent the type and level of questioning on the HTL. Also that exam is more heavy on the application, troubleshooting and synthesis. There are some operations questions on there as well that do not appear on the HT exam. I like the CAP website for information for background on compliance and higher level functions. The NSH study materials I also feel are very helpful for content review, and available from their website.Here are some other good resources on the web that I think make for a good review: Tissue IDhttp://www.lumen.luc.edu/lumen/MedEd/Histo/frames/histo_frames.htmlhttp://www.siumed.edu/~dking2/index.htmSpecialshttp://stainsfile.info/StainsFile/jindex.html Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > From: LStadler@cbiolabs.com > To: histonet@lists.utsouthwestern.edu > Date: Fri, 11 Nov 2011 14:50:41 +0000 > Subject: [Histonet] Exam Prep Info/Guidance/Suggestions > > All ~ > > I am about to begin preparing for the HTL certification exam. (I supposse it is only fair to mention that this is will be my second attempt. My first exam taken last month with a score of 382 out of a minimum passing score of 400). > > As a "research" Histology Technician, a good majority of the material covered on the exam was new to me. My prep for the first exam was to read "Histotechnology, A Self-Instructional Text, 3rd Edtion" by Carson and Hladik and then answer a lot of "practice questions" from the Board of Registry Second Edition Practice Questions book. I focused mostly on the special staining info and images because of the high concentration of questions on the exam as well as my limited professionnal exposure to these...unfortunately, it was still the area i did most poorly in on the exam. > > In order to go a little more in depth the second time around, my plan is to is to re-read each chapter ( of Carson), then assess my knowledge by being sure I can do each of the "Chapter Objectives" and then answer the questions in the self-assessement workbook, then answer the questions on that particular chapter topic from the Seond Edition of the Board of Registry Study Guide, Practice Questions for the Histotechnology Exams (The Purple Book). I have other textbooks at my disposal, but as a mostly visual learner, I find Carson's approach to best get the information into my brain! Again, once I feel comfortable with all the basic knowledge, I would focus mostly on staining. > > Specifically, I would like feedback from anyone who has recently passed the exam about my approach, and suggestions for other ideas. Also, I have very limited CAP/Joint Comission type knowlege and wonder if anyone can offer a resource for "basics" for someone like me in research who is not presented with the info and regulations on a daily basis! Also, any suggestions for images online of special stains would be a great resource for me. > > Thanks in advance for any and all info! > > Lyn > > > > > > This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brannon <@t> alliedsearchpartners.com Fri Nov 11 10:03:31 2011 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Fri Nov 11 10:03:48 2011 Subject: [Histonet] Histology Manager Needed in Baton Rouge, LA Message-ID: Allied Search Partners has been retained in the search for a Histology Supervisor for a Baton Rouge, LA client. Position Title: Histology Supervisor/Manager Schedule: Full Time/Permanent Lab Environment: Private Lab, State of the art Equipment, Family Oriented Environment Summary: Management of Histology Laboratory Responsibilities: -Supervision of 10 histology technicians and 2 lab assistants -Prioritizes day to day workflow and ensures standards of accuracy, quality, and turn around times are met -Adjusts schedules and provides input for assessment or disciplinary action as needed Qualifications: HTL or HT (ASCP) certification required Previous supervisory, IHC and Special Staining experience preferred 2 years previous supervisory experience preferred Benefits: Fully benefited with Medical Insurance, Paid Time Off, Relocation Assistance, and more to be discussed with the HR manager if phone interview is requested. We offer a competitive benefit package, including 401K plan. Salary is commensurate with experience. To apply: 1. Send Resume to Brannon@alliedsearchpartners.com 2. Salary Expectations 3. Availability for a short phone screen with one of our recruiters Be sure to visit our website www.alliedsearchpartners.com to submit your job search request, refer a friend for a generous bonus, and have your resume reviewed for free by our career advisors. -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From ArtimK <@t> slhn.org Fri Nov 11 10:16:40 2011 From: ArtimK <@t> slhn.org (Artim, Kimberly) Date: Fri Nov 11 10:16:46 2011 Subject: [Histonet] JAZF1 antibody Message-ID: <9E67FDD215B226448638018A82B952BD026A1D7B6E24@EXCHANGE.slhn.org> Is anyone aware of a reference or research facility that does JAZF1 by IHC or PCR? Kimberly Artim, AST, HT (ASCP) Technical Coordinator, Anatomic Pathology St Lukes Hospital & Health Network 801 Ostrum Street Bethlehem, PA 18015 610-954-4832 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From joelleweaver <@t> hotmail.com Fri Nov 11 10:47:09 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Nov 11 10:47:15 2011 Subject: [Histonet] Exam Prep Info/Guidance/Suggestions In-Reply-To: <98CC14B915EBA84B9A326D45CC3C1DEC3AB8FFE5@cbiolabs05.CBiolabs.local> References: <98CC14B915EBA84B9A326D45CC3C1DEC3AB8FF5E@cbiolabs05.CBiolabs.local> , <98CC14B915EBA84B9A326D45CC3C1DEC3AB8FFE5@cbiolabs05.CBiolabs.local> Message-ID: LynI am happy if I can help. It is a difficult exam, with one of the lowest pass rates and you got really close on your first try so don't be hard on yourself. I wish you the best of luck, and I am confident you will be successful on your next attempt! Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From: LStadler@cbiolabs.com To: joelleweaver@hotmail.com Subject: RE: [Histonet] Exam Prep Info/Guidance/Suggestions Date: Fri, 11 Nov 2011 16:28:44 +0000 Joelle ~ Thank you so much for your detailed and thoughtful response! This is most greatly appreciated and I am so grateful for histonet for this kind of professional interaction and support. I will take your info into account and be sure to let you know how it goes! Lyn From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Friday, November 11, 2011 10:25 AM To: Lyn Stadler; Histonet Subject: RE: [Histonet] Exam Prep Info/Guidance/Suggestions Lyn I compliment you on your organized approach. I did not take the exam "recently", I am old now! But as part of my previous job I spent a lot of time analyzing the content and exam topics for the HT exam. If you look at both outlines (HT and HTL) you will see that they cover a broad scope of histotechnology. Many people assume that it is mostly "routine" histology, but in fact they throw some obscure things in there and some histology speciality practices, and my understanding from a teleconference on the topic from the exam preparers and working with ASCP and NAACLS, is that they want to encompass the full spectrum of possible environments where histologists may work.There are key differences between clinical and research environments, but the basic histology theory is the same, just practices and emphasis differences. This makes sense if you think about their purpose(s). So if you understand the fundamentals you should be able to move easily between the environments and I believe that is what they are going for. Anyhow, I think that the newest Carson edition is good, but my personal feeling is that it is quite heavy on the very routine and most common special stains only, and though it is much better than the previous ones ( which I felt were very superficial), it is still mostly routine since it is meant to be an introductory text. I recommend the Sheehan and Hrapchak text for studying the less common special stains, if you can utilize this for the underlying chemistry and theory. Though bear in mind that this text is quite old now and some techniques presented are pretty outdated, but you can get the theory from it. It is not as "reader" friendly, and less pictures, mostly diagrams if any, but the chemistry is laid out pretty well in my opinion, though you have to dig around a bit. My advice to people has always been to make some attempt to understand the underlying chemistry so that you are not trying to memorize stains. They can be grouped according to chemical interaction and target tissue element for study purposes. Then you will not be thrown off by "modifications" and variations so much for histochemical stains. I organized it this way when I taught chemistry of stains and this seems to help people digest the information without getting overwhelmed by all the stains out there. The web is good these days for providing practice images and gets you used to seeming them on a monitor- though there is no replacement for spending time at the microscope. I think being strong in tissue identification also helps immensly for both HC and IHC staining, so any time you spend with that at the scope is great in my opinion. The BOR study guide is good to get you used to the wording and presentation of the questions that are used ( being retired or not used exam questions), and gives you some idea of the scope of the content. The more difficult questions are marked with an * in the guide, and they represent the type and level of questioning on the HTL. Also that exam is more heavy on the application, troubleshooting and synthesis. There are some operations questions on there as well that do not appear on the HT exam. I like the CAP website for information for background on compliance and higher level functions. The NSH study materials I also feel are very helpful for content review, and available from their website. Here are some other good resources on the web that I think make for a good review: Tissue ID http://www.lumen.luc.edu/lumen/MedEd/Histo/frames/histo_frames.html http://www.siumed.edu/~dking2/index.htmSpecials http://stainsfile.info/StainsFile/jindex.html Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > From: LStadler@cbiolabs.com > To: histonet@lists.utsouthwestern.edu > Date: Fri, 11 Nov 2011 14:50:41 +0000 > Subject: [Histonet] Exam Prep Info/Guidance/Suggestions > > All ~ > > I am about to begin preparing for the HTL certification exam. (I supposse it is only fair to mention that this is will be my second attempt. My first exam taken last month with a score of 382 out of a minimum passing score of 400). > > As a "research" Histology Technician, a good majority of the material covered on the exam was new to me. My prep for the first exam was to read "Histotechnology, A Self-Instructional Text, 3rd Edtion" by Carson and Hladik and then answer a lot of "practice questions" from the Board of Registry Second Edition Practice Questions book. I focused mostly on the special staining info and images because of the high concentration of questions on the exam as well as my limited professionnal exposure to these...unfortunately, it was still the area i did most poorly in on the exam. > > In order to go a little more in depth the second time around, my plan is to is to re-read each chapter ( of Carson), then assess my knowledge by being sure I can do each of the "Chapter Objectives" and then answer the questions in the self-assessement workbook, then answer the questions on that particular chapter topic from the Seond Edition of the Board of Registry Study Guide, Practice Questions for the Histotechnology Exams (The Purple Book). I have other textbooks at my disposal, but as a mostly visual learner, I find Carson's approach to best get the information into my brain! Again, once I feel comfortable with all the basic knowledge, I would focus mostly on staining. > > Specifically, I would like feedback from anyone who has recently passed the exam about my approach, and suggestions for other ideas. Also, I have very limited CAP/Joint Comission type knowlege and wonder if anyone can offer a resource for "basics" for someone like me in research who is not presented with the info and regulations on a daily basis! Also, any suggestions for images online of special stains would be a great resource for me. > > Thanks in advance for any and all info! > > Lyn > > > > > > This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From NHeath <@t> Lifespan.org Fri Nov 11 12:01:16 2011 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Fri Nov 11 12:01:28 2011 Subject: [Histonet] Exam Prep Info/Guidance/Suggestions In-Reply-To: References: <98CC14B915EBA84B9A326D45CC3C1DEC3AB8FF5E@cbiolabs05.CBiolabs.local>, <98CC14B915EBA84B9A326D45CC3C1DEC3AB8FFE5@cbiolabs05.CBiolabs.local> Message-ID: <130E8991F210424096EFC6F42EA33B2408505403@LSCOEXCH1.lsmaster.lifespan.org> Hi Lyn, Go to our website www.rihisto.org and go to our links page where you will find the histology-world website and they have loads of histo info! Good luck on your HTL!! Nancy Heath, HT (ASCP) President - Rhode Island Society for Histotechnology :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Friday, November 11, 2011 11:47 AM To: lstadler@cbiolabs.com; histonet-bounces@lists.utsouthwestern.edu; Histonet Subject: RE: [Histonet] Exam Prep Info/Guidance/Suggestions LynI am happy if I can help. It is a difficult exam, with one of the lowest pass rates and you got really close on your first try so don't be hard on yourself. I wish you the best of luck, and I am confident you will be successful on your next attempt! Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From: LStadler@cbiolabs.com To: joelleweaver@hotmail.com Subject: RE: [Histonet] Exam Prep Info/Guidance/Suggestions Date: Fri, 11 Nov 2011 16:28:44 +0000 Joelle ~ Thank you so much for your detailed and thoughtful response! This is most greatly appreciated and I am so grateful for histonet for this kind of professional interaction and support. I will take your info into account and be sure to let you know how it goes! Lyn From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Friday, November 11, 2011 10:25 AM To: Lyn Stadler; Histonet Subject: RE: [Histonet] Exam Prep Info/Guidance/Suggestions Lyn I compliment you on your organized approach. I did not take the exam "recently", I am old now! But as part of my previous job I spent a lot of time analyzing the content and exam topics for the HT exam. If you look at both outlines (HT and HTL) you will see that they cover a broad scope of histotechnology. Many people assume that it is mostly "routine" histology, but in fact they throw some obscure things in there and some histology speciality practices, and my understanding from a teleconference on the topic from the exam preparers and working with ASCP and NAACLS, is that they want to encompass the full spectrum of possible environments where histologists may work.There are key differences between clinical and research environments, but the basic histology theory is the same, just practices and emphasis differences. This makes sense if you think about their purpose(s). So if you understand the fundamentals you should be able to move easily between the environments and I believe that is what they are going for. Anyhow, I think that the newest Carson edition is good, but my personal feeling is that it is quite heavy on the very routine and most common special stains only, and though it is much better than the previous ones ( which I felt were very superficial), it is still mostly routine since it is meant to be an introductory text. I recommend the Sheehan and Hrapchak text for studying the less common special stains, if you can utilize this for the underlying chemistry and theory. Though bear in mind that this text is quite old now and some techniques presented are pretty outdated, but you can get the theory from it. It is not as "reader" friendly, and less pictures, mostly diagrams if any, but the chemistry is laid out pretty well in my opinion, though you have to dig around a bit. My advice to people has always been to make some attempt to understand the underlying chemistry so that you are not trying to memorize stains. They can be grouped according to chemical interaction and target tissue element for study purposes. Then you will not be thrown off by "modifications" and variations so much for histochemical stains. I organized it this way when I taught chemistry of stains and this seems to help people digest the information without getting overwhelmed by all the stains out there. The web is good these days for providing practice images and gets you used to seeming them on a monitor- though there is no replacement for spending time at the microscope. I think being strong in tissue identification also helps immensly for both HC and IHC staining, so any time you spend with that at the scope is great in my opinion. The BOR study guide is good to get you used to the wording and presentation of the questions that are used ( being retired or not used exam questions), and gives you some idea of the scope of the content. The more difficult questions are marked with an * in the guide, and they represent the type and level of questioning on the HTL. Also that exam is more heavy on the application, troubleshooting and synthesis. There are some operations questions on there as well that do not appear on the HT exam. I like the CAP website for information for background on compliance and higher level functions. The NSH study materials I also feel are very helpful for content review, and available from their website. Here are some other good resources on the web that I think make for a good review: Tissue ID http://www.lumen.luc.edu/lumen/MedEd/Histo/frames/histo_frames.html http://www.siumed.edu/~dking2/index.htmSpecials http://stainsfile.info/StainsFile/jindex.html Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > From: LStadler@cbiolabs.com > To: histonet@lists.utsouthwestern.edu > Date: Fri, 11 Nov 2011 14:50:41 +0000 > Subject: [Histonet] Exam Prep Info/Guidance/Suggestions > > All ~ > > I am about to begin preparing for the HTL certification exam. (I supposse it is only fair to mention that this is will be my second attempt. My first exam taken last month with a score of 382 out of a minimum passing score of 400). > > As a "research" Histology Technician, a good majority of the material covered on the exam was new to me. My prep for the first exam was to read "Histotechnology, A Self-Instructional Text, 3rd Edtion" by Carson and Hladik and then answer a lot of "practice questions" from the Board of Registry Second Edition Practice Questions book. I focused mostly on the special staining info and images because of the high concentration of questions on the exam as well as my limited professionnal exposure to these...unfortunately, it was still the area i did most poorly in on the exam. > > In order to go a little more in depth the second time around, my plan is to is to re-read each chapter ( of Carson), then assess my knowledge by being sure I can do each of the "Chapter Objectives" and then answer the questions in the self-assessement workbook, then answer the questions on that particular chapter topic from the Seond Edition of the Board of Registry Study Guide, Practice Questions for the Histotechnology Exams (The Purple Book). I have other textbooks at my disposal, but as a mostly visual learner, I find Carson's approach to best get the information into my brain! Again, once I feel comfortable with all the basic knowledge, I would focus mostly on staining. > > Specifically, I would like feedback from anyone who has recently passed the exam about my approach, and suggestions for other ideas. Also, I have very limited CAP/Joint Comission type knowlege and wonder if anyone can offer a resource for "basics" for someone like me in research who is not presented with the info and regulations on a daily basis! Also, any suggestions for images online of special stains would be a great resource for me. > > Thanks in advance for any and all info! > > Lyn > > > > > > This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LINDA.MARGRAF <@t> childrens.com Fri Nov 11 14:01:27 2011 From: LINDA.MARGRAF <@t> childrens.com (Linda Margraf) Date: Fri Nov 11 14:01:52 2011 Subject: [Histonet] Unsubscribing from Histonet Message-ID: Dear Histonetters: Hi, I haven't put out many messages on the list recently. I have just been quietly administering the list (and occasionally removing people who ask to be unsubscribed as my time allows). By the way, Histonet is coming up on its 16th year anniversary (wow!) and there are currently 3543 members on the list (from >30 countries as of my last tally). As it keeps coming up, if you wish to unsubscribe or modify your account options, go to this link: http://lists.utsouthwestern.edu/mailman/options/histonet You will need to request your password if you don't know or remember it. I tried to get this link added to the bottom of all the messages but apparently that is not possible with the current software. If you have trouble getting off the list, let me know. If your email address has been changed at your organization, you may have trouble posting messages or unsubscribing. If so, let me know (but provide me with the old address that is on the membership list) and I'll try to help. Thanks, Linda M Histonet administrator Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail

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disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
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From POWELL_SA <@t> mercer.edu Fri Nov 11 14:34:02 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Nov 11 14:34:09 2011 Subject: [Histonet] RE: Unsubscribing from Histonet In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE24A9E221CCB@MERCERMAIL.MercerU.local> Thank you Linda for providing us with such a wonderful tool for the histology community. I am sure all on the list appreciate it as well. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Friday, November 11, 2011 3:01 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unsubscribing from Histonet Dear Histonetters: Hi, I haven't put out many messages on the list recently. I have just been quietly administering the list (and occasionally removing people who ask to be unsubscribed as my time allows). By the way, Histonet is coming up on its 16th year anniversary (wow!) and there are currently 3543 members on the list (from >30 countries as of my last tally). As it keeps coming up, if you wish to unsubscribe or modify your account options, go to this link: http://lists.utsouthwestern.edu/mailman/options/histonet You will need to request your password if you don't know or remember it. I tried to get this link added to the bottom of all the messages but apparently that is not possible with the current software. If you have trouble getting off the list, let me know. If your email address has been changed at your organization, you may have trouble posting messages or unsubscribing. If so, let me know (but provide me with the old address that is on the membership list) and I'll try to help. Thanks, Linda M Histonet administrator Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail

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disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
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please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at
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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Fri Nov 11 14:40:49 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Nov 11 14:40:56 2011 Subject: [Histonet] RE: Unsubscribing from Histonet In-Reply-To: <9BF995BC0E47744E9673A41486E24EE24A9E221CCB@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE24A9E221CCB@MERCERMAIL.MercerU.local> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640844197C98@CHEXCMS10.one.ads.che.org> >From me too!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Friday, November 11, 2011 15:34 To: Linda Margraf Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Unsubscribing from Histonet Thank you Linda for providing us with such a wonderful tool for the histology community. I am sure all on the list appreciate it as well. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Friday, November 11, 2011 3:01 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unsubscribing from Histonet Dear Histonetters: Hi, I haven't put out many messages on the list recently. I have just been quietly administering the list (and occasionally removing people who ask to be unsubscribed as my time allows). By the way, Histonet is coming up on its 16th year anniversary (wow!) and there are currently 3543 members on the list (from >30 countries as of my last tally). As it keeps coming up, if you wish to unsubscribe or modify your account options, go to this link: http://lists.utsouthwestern.edu/mailman/options/histonet You will need to request your password if you don't know or remember it. I tried to get this link added to the bottom of all the messages but apparently that is not possible with the current software. If you have trouble getting off the list, let me know. If your email address has been changed at your organization, you may have trouble posting messages or unsubscribing. If so, let me know (but provide me with the old address that is on the membership list) and I'll try to help. Thanks, Linda M Histonet administrator Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail

This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
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individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further
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disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
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please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at
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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From jkiernan <@t> uwo.ca Fri Nov 11 15:48:03 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Nov 11 15:48:12 2011 Subject: [Histonet] Chicago Sky Blue stain vs. KOH - striking comparison In-Reply-To: References: Message-ID: <7690d00e91f56.4ebd51c3@uwo.ca> The names Chicago blue and sky blue have been applied to various disazo dyes used in the past for direct dyeing of cotton. The name Chicago sky blue does not appear as a principal name or a synonym in either the 9th (1977) or the 10th (2002) editions of Conn's Biological Stains. In Conn9, there is a reference for staining fungi (Kuranz 1964) with ink blue, which was believed to be Chicago blue 6B (CI 24410, Direct blue 1). This dye has also been used to quench autofluorescence prior to immunofluorescent staining, often as pontamine sky blue 6B or Niagara blue 6B. Closely related dyes are Chicago blue 4B (CI 24395, also called benzo sky blue 2B and brilliant benzo blue 6B) and benzo sky blue (CI 24400, Direct blue 15, also called sky blue and pure blue). Probably Chicago blue 6B (CI 24410, Direct blue 1) is the only one of these likely to be available. In the 1970s it was still an article of commerce with more than 70 brand names, but by 2002 it was no longer used industrially. The CI number and application name are the best identifiers, but remember that dyes often are mixtures and sometimes are mislabelled. None of these blue disazo dyes are certified by the Biological Stain Commission. John Kiernan Anatomy & Cell Biology UWO, London, Canada. = = = On 10/11/11, "Harrison, Sandra C." wrote: > > Does anyone have ordering information for a stain called Chicago Sky > Blue(CSB)? I just saw an article in the NOV issue of ASCP LABMEDICINE > (page 649) comparing KOH vs. CSB and, wow, is that a beautiful stain. > > > > The results were dramatic, with about a 25-50% increase in the > Dermatophytes identified and about a 5X increase in the Pityriasis > versicolor identification. > > > > Thanks, > > > > Sandy C. Harrison, HTL (ASCP) > > Histology Supervisor > > From jkiernan <@t> uwo.ca Sat Nov 12 01:03:18 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Nov 12 01:03:24 2011 Subject: [Histonet] Histonet 15+ years old, thanks to Dr Linda Margraf In-Reply-To: References: Message-ID: <7610eec88e072.4ebdd3e6@uwo.ca> Linda, this is a great achievement. More than 15 years! I found Histonet by way of a reference on a newsgroup (are there still such things?) perhaps in 1996. I didn't realize at the time that Histonet was almost a newborn institution. There already were regular contributors, and some of them are still with us. Histonet took off as a great medium for asking and answering questions in histotechnology. This is a field that embraces a wide range of expertise including manual skills, understanding of physical, chemical, immunological and molecular biological principles, and coping with regulatory issues. You set up and you still maintain a noteworthy medium for enquiry, discussion, teaching, and often argument in a generally friendly internet community that includes students, professors, clinicians, academic researchers, and especially technicians at all levels from beginners to post-PhD. Histonet is an egalitarian forum for all who use microscopes to look at tissues. My congratulatory message to Linda Margraf could spawn a large number of others, but please desist! Histonet already is swamped with trivial comments that clog our mailboxes. John Kiernan (Anatomy, UWO, London, Canada) Histonetter on and off for about 15 years. = = = On 11/11/11, Linda Margraf wrote: > > Dear Histonetters: > > Hi, I haven't put out many messages on the list recently. I have just been quietly administering the list (and occasionally removing people who ask to be unsubscribed as my time allows). By the way, Histonet is coming up on its 16th year anniversary (wow!) and there are currently 3543 members on the list (from >30 countries as of my last tally). > > As it keeps coming up, if you wish to unsubscribe or modify your account options, go to this link: > > > http://lists.utsouthwestern.edu/mailman/options/histonet > > You will need to request your password if you don't know or remember it. I tried to get this link added to the bottom of all the messages but apparently that is not possible with the current software. If you have trouble getting off the list, let me know. If your email address has been changed at your organization, you may have trouble posting messages or unsubscribing. If so, let me know (but provide me with the old address that is on the membership list) and I'll try to help. > Thanks, > Linda M > Histonet administrator > > Please consider the environment before printing this e-mail > > This e-mail, facsimile, or letter and any files or attachments transmitted with it contains > information that is confidential and privileged. This information is intended only for the use of the > individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further > disclosures are prohibited without proper authorization. If you are not the intended recipient, any > disclosure, copying, printing, or use of this information is strictly prohibited and possibly a > violation of federal or state law and regulations. If you have received this information in error, > please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at > privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all > applicable privileges related to this information. > > Please consider the environment before printing this e-mail
>
> > This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
> information that is confidential and privileged. This information is intended only for the use of the
> individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further
> > disclosures are prohibited without proper authorization. If you are not the intended recipient, any
> disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
> violation of federal or state law and regulations. If you have received this information in error,
> please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at
> privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all
> applicable privileges related to this information.
> >
> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From mdpraet <@t> gmail.com Sat Nov 12 08:14:44 2011 From: mdpraet <@t> gmail.com (mequita praet) Date: Sat Nov 12 08:14:49 2011 Subject: [Histonet] Exam Prep Message-ID: Hi Lyn, I saw your question on histonet about studying for the ASCP exam. My suggestion is for you to go to www.nsh.org and order the self assessment booklets or CD's. If you can afford them get all of them. If not get the ones you are weakest in. They are great. They have been updated and are current. A lot of the exam questions come straight from these booklets. They will be a great addition to your personal library. Study, study, study and you will be successful! I have been in this field for over 40 years and I have great confidence in you. You have taken great steps toward your goal by reaching out to your peers. I wish you much success. Meuita Praet, HTL(ASCP)SLS From BoozerKA <@t> ah.org Sat Nov 12 09:44:38 2011 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Sat Nov 12 09:44:48 2011 Subject: [Histonet] I am receiving 2 of every emails to boozerka@ah.org. Is it something I did? Message-ID: <4EBE23E5.4AA8.00C0.1@ah.org> I am receiving 2 of every emails to boozerka@ah.org. Is it something I did? Thank you, Kathy Boozer From andreahooper <@t> rocketmail.com Sat Nov 12 10:15:42 2011 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Sat Nov 12 10:15:49 2011 Subject: [Histonet] Problems with Frozen tissues In-Reply-To: <1320191637.61068.YahooMailNeo@web112304.mail.gq1.yahoo.com> References: <1320076911.80808.YahooMailNeo@web112303.mail.gq1.yahoo.com> <9DF797D618351549B984596F01A1FE1D01FCC75D@murmx.mcri.edu.au> <1320191637.61068.YahooMailNeo@web112304.mail.gq1.yahoo.com> Message-ID: Hi Kim, I routinely dry my cryosections after sectioning for a few hours, then fix in the fixative required for the assay with high quality, almost FFPE like, reproducible & publishable results. Do you know for certain drying reduces quality of results? Do you have data or publications you can share with me? Andrea Hooper Sent from my iPhone On Nov 1, 2011, at 7:53 PM, Kim Donadio wrote: > may I ask why you are not fixing them? Even the stain the 1st gentlman was doing(Beta Gal) recomended to have a formalin fixation apllied first. > > There are many ways to get to the same show as we all know, But in my experiance air drying frozen sections will dry out and cells can autolyse as the process of autolysation has not been stopped, also vacouls in the tissue can develope from the process of dehydration without the use of fixation< sorry for spelling, on lap top on dark porch. > > Are you trying to minumize usage of alcohols or other fixatives? > > PLease explain to me if you would because I have cut just about all kinds of tissues for all kinds of procedures and never have I heard to keep a fresh section out for an hour before staining and without a fixative. I love ot learn new things ) > > Thanks > > Kim > > > ________________________________ > From: Daniela Bodemer > To: Kim Donadio ; Igor Deyneko ; Histonet@lists.utsouthwestern.edu > Sent: Tuesday, November 1, 2011 6:16 PM > Subject: RE: [Histonet] Problems with Frozen tissues > > Our tissues are frozen in OCT. I use Super Frost Plus slides from Thermo Scientific and store the sections in the fridge until I need them. After leaving them out on the bench for an hour I start my H&E with 1 min running water and so on. I rarely lose sections with these slides. > > Daniela > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio > Sent: Tuesday, 1 November 2011 3:02 AM > To: Igor Deyneko; Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Problems with Frozen tissues > > Ive never just air dried my frozen sections. always put them in a fixative such as pen fix, a alcohol and or formalin mixture, something( depends on what your going to look for, test etc). That with using charged slides and never had too many problems with this. > > Kim > > > ________________________________ > From: Igor Deyneko > To: Histonet@lists.utsouthwestern.edu > Sent: Monday, October 31, 2011 9:56 AM > Subject: [Histonet] Problems with Frozen tissues > > I'm looking for some advice on frozen tissues. This is the first time I'm doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut well onto special Gold Plus slides from Fisher. Then, when I was ready to stain the slides, i air dried them fro an hour and wanted to do H&E and Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on preventing this mischief? > Thank you in advance. > Igor Deyneko > Infinity Pharmaceuticals > Cambridge, MA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > > If you have any question, please contact MCRI IT Helpdesk for further assistance. > ______________________________________________________________________ > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Sat Nov 12 19:49:47 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sat Nov 12 19:49:57 2011 Subject: [Histonet] Exam Prep In-Reply-To: Message-ID: The NSH has changed the format for the Self-Assessment Series. It is now offered on-line. You can purchase the whole series or individual modules. They were selling off the booklets that they had left, but I don't see them on the website anymore. There is no CD available. mequita praet Sent by: histonet-bounces@lists.utsouthwestern.edu 11/12/2011 06:17 AM To lstadler@cbiolabs.com cc histonet@lists.utsouthwestern.edu Subject [Histonet] Exam Prep Hi Lyn, I saw your question on histonet about studying for the ASCP exam. My suggestion is for you to go to www.nsh.org and order the self assessment booklets or CD's. If you can afford them get all of them. If not get the ones you are weakest in. They are great. They have been updated and are current. A lot of the exam questions come straight from these booklets. They will be a great addition to your personal library. Study, study, study and you will be successful! I have been in this field for over 40 years and I have great confidence in you. You have taken great steps toward your goal by reaching out to your peers. I wish you much success. Meuita Praet, HTL(ASCP)SLS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Sun Nov 13 07:44:25 2011 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Sun Nov 13 07:44:29 2011 Subject: [Histonet] ? on frozen tissue artifacts In-Reply-To: References: Message-ID: <2AACB232-2FBC-4EB0-B303-84E8CF0AB6AB@rocketmail.com> Sounds to me Definitely more likely to be the freezing process. Do you know their process? Andrea On Oct 22, 2011, at 5:05 PM, histopath101@gmail.com wrote: > I never said these were muscles. These are tumors removed and frozen the day before and sent to us on dry ice. We know not to use frost-free freezers! > > On , "Esparza, Sandra" wrote: >> I have never put my muscles in a -20 we use -70 to -80 freezer and have > > >> not had a problem with artifact. Is your freezer frost free? > > > > > >> Sandra > > >> Sandra Esparza HT(ASCP)QIHC > > >> Lead Technologist > > >> Dell Children's Medical Center of Central Texas > > >> 512-324-0000 x87061 > > >> sesparza@seton.org > > > > > >> -----Original Message----- > > >> From: histonet-bounces@lists.utsouthwestern.edu > > >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > >> histopath101@gmail.com > > >> Sent: Friday, October 21, 2011 8:44 AM > > >> To: Histonet@lists.utsouthwestern.edu > > >> Subject: [Histonet] ? on frozen tissue artifacts > > > > > >> I work in a reference lab and some of our frozen sections exhibit severe > > > > > >> freeze artifact (ice crystals). The client claims it's something we're > > >> doing and we say they are not freezing them properly. We receive the > > >> specimens on dry ice and store them in a -20C freezer until we section > > >> them > > >> (no more than 1-2 days later). We never allow the tissues to thaw. My > > >> question: Can freeze artifact occur AFTER properly snap-freezing tissue > > >> or > > >> does this artifact ONLY occur during the initial preezing procedure? > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet@lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Sun Nov 13 09:06:01 2011 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Sun Nov 13 09:06:12 2011 Subject: [Histonet] Thick cryosections In-Reply-To: <7922198191carmaga6@uv.es> References: <7922198191carmaga6@uv.es> Message-ID: <6CB1D470-73B8-49CA-9BBB-B3A08596F7F5@rocketmail.com> Sorry for the late response. I have done immunofluorescence on 50 um cryosections of mouse host tumors and decalcified bone on super frost Plus slides. I melt them to the slide using body heat then dry them at RT for a few hours before storing at -80 deg C. They do not come off the slide. What step do your sections come off the slide? Perhaps you can optimize your protocol to avoid this. Andrea On Oct 11, 2011, at 11:11 AM, "Carmen Maria Garcia Pascual" wrote: > > Good afternoon everyone! > > I have made 50um mice ovarian cryosections and I have used superfrost > plus slides. But some of them fall of the slides when I perfomed the > immuno, one colleague has told me that this is because the cuts are too > thick, and I think she is rigth. I don't know if there is some thing > that I can make to avoid this, like using another kind of slides... > > Thanks!!!!!!!!!!! > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Sun Nov 13 09:58:02 2011 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Sun Nov 13 09:58:07 2011 Subject: [Histonet] Isotype specific polymers In-Reply-To: <3FEFF18FF4E1914A9AB7D8498591BE8610F149E424@VEXCCR02.phsabc.ehcnet.ca> References: <3FEFF18FF4E1914A9AB7D8498591BE8610F149E424@VEXCCR02.phsabc.ehcnet.ca> Message-ID: <530E32FB-131E-4DC8-893F-5BED1266D106@rocketmail.com> Not sure, but perhaps you can buy a rabbit anti mouse IgG2a link antibody for example and then use a rabbit polymer. Andrea On Oct 5, 2011, at 4:19 PM, "Milne, Katy" wrote: > Hi everyone, > > I'm curious if there is such a thing as isotype specific polymers (ie will react with mouse IgG2a but not IgG1). Does anyone make such a thing? > > Thanks, > Katy > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Sun Nov 13 10:09:23 2011 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Sun Nov 13 10:09:27 2011 Subject: [Histonet] Decal retrieving In-Reply-To: References: <1319656942.77550.YahooMailNeo@web125302.mail.ne1.yahoo.com> Message-ID: Does anyone have experience with epitopes that are "retrieved" after decal? We have used three separate buffers (HCl, formic acid, formic acid/EDTA) and all retrieve. This is with three different proteins we are staining for, and we were curious if it is a common occurrence. Andrea From andreahooper <@t> rocketmail.com Sun Nov 13 10:21:14 2011 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Sun Nov 13 10:21:19 2011 Subject: [Histonet] F4/80 Message-ID: Has anyone stained NOD/SCID lungs for F4/80 or any macrophage marker? What is the expected staining pattern? Andrea From joelleweaver <@t> hotmail.com Mon Nov 14 08:11:19 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Nov 14 08:11:24 2011 Subject: [Histonet] RE: HTL Exam In-Reply-To: <15319822.109648.1321040304017.JavaMail.root@mail3d.brinkster.com> References: <341398.109629.1321040183464.JavaMail.root@mail3d.brinkster.com>, <15319822.109648.1321040304017.JavaMail.root@mail3d.brinkster.com> Message-ID: AdrienneThanks for your thoughts, however I was not in need of any study help, having passed the exam some time ago, but was posting in response to students questions to try to help and support them. I think your system is good if your goal is memorization. My personal feeling is that while this may allow you to select the correct answer on any MC test in the short term; if you only memorize information it will leave short term recall very quickly, and you will be lacking when called upon to use this information for application, synthesis, troubleshooting etc., in a real lab situation. Memorization is a learning first step, but good to move beyond this level of understanding in your learning process. I am glad that you were able to pass, and I hope you go on to build on your learning through your work in the lab. Best of luck. Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > Date: Fri, 11 Nov 2011 12:38:24 -0700 > From: aaperghis@uspath.com > To: joelleweaver@hotmail.com > Subject: HTL Exam > > Hi Joelle, > > > I took the HTL exam in April. I thought I did terribly but ended up passing (go figure). > > I honestly think it's a lot of memorization. > > I got the flash-cards that compliment the Carson text. Some images were repeats of the book but most were new. And it was an excellent study guide for questions like: what is this stain, what's wrong with this stain/section, what's the best fixative for this stain, etc. I also made my own flashcards, of which I had probably around 500. Not an easy task. > > I read and high-lighted the Carson and Barncroft text. Then I went through and made an outline of the text (a loooong outline). > After reviewing that a few times, I made my flashcards. > > Going through the flashcards over the weeks, I could eventually cut out the ones that I knew the answers to and kept studying the more difficult ones. > > The Barncroft text was an excellent supplemental for Carson and also had nice images. > > If you would like my outline, let me know and I will send it over. $500. Kidding. > > Best of luck! > > Adrienne > > -- > > > > Adrienne Kavanagh HTL (ASCP) > US PATH > 30 W. Century Road > Suite 255 > Paramus NJ 07652 From a.boanas <@t> epistem.co.uk Mon Nov 14 09:13:05 2011 From: a.boanas <@t> epistem.co.uk (Adam Boanas) Date: Mon Nov 14 09:13:13 2011 Subject: [Histonet] Whiskers Message-ID: <176C97AAA877D24798ED7376652D0FD815AEE4F263@SRVEXCH02.epistem.local> Hello, I am currently sectioning individual rat whiskers and follicles and am having some trouble obtaining clean sections from within the centre of the whisker (and intact bulb) without the brittle hair falling out of the block or splintering. Does anyone know of a method that I could use to soften the keratin prior to embedding? I am thinking 10% Sodium Hydroxide or 5% Ammonium Hydroxide but do not really know about appropriate timings for such a small tissue. Many thanks, Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX From birems2 <@t> yahoo.com Mon Nov 14 09:29:49 2011 From: birems2 <@t> yahoo.com (Remedy Bi) Date: Mon Nov 14 09:29:52 2011 Subject: [Histonet] using Aquoes mounting media Message-ID: <1321284589.35355.YahooMailClassic@web45516.mail.sp1.yahoo.com> Hello friends i wish to fine out how to use the aqueous mounting media. thanks alot From joelleweaver <@t> hotmail.com Mon Nov 14 09:46:30 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Nov 14 09:46:35 2011 Subject: [Histonet] RE: HTL Exam In-Reply-To: <654263.115602.1321279721258.JavaMail.root@mail3d.brinkster.com> References: <15319822.109648.1321040304017.JavaMail.root@mail3d.brinkster.com>, <654263.115602.1321279721258.JavaMail.root@mail3d.brinkster.com> Message-ID: No harm done. I believe that you meant the Bancroft text, and it is also good and similar in content to the Sheehan & Hrapchak as well as the Pierce and Luna publication(s) . All would suffice in my opinion, as a compliment to Carson, it is more or less a matter of opinion and preference, though you have to keep in mind the publication timelines of those. Your flashcards would be helpful to students I believe, though in my opinion the process of creating the flashcards or outlines yourself is probably as valuable to the intial memorization, as using them as memorization tools.There are considerably more good resources available now than in the past, and whatever methods work best for your learning- go for it. Given my own past experiences, I merely emphasize the practical application of the knowledge in the lab as the final, yet most important, step. However, I recognize and encourage ANYONE who attempts the HTL, however they choose to pursue it, we really need more educated, knowledgable people in the hiring pool! Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > Date: Mon, 14 Nov 2011 07:08:41 -0700 > From: aaperghis@uspath.com > To: joelleweaver@hotmail.com > Subject: Re: HTL Exam > > I just realized I sent this to you instead of the one asking about the exam. > > My apologies! > > > > ----- Original Message ----- > From: "Adrienne Aperghis Kavanagh" > To: joelleweaver@hotmail.com > Sent: Friday, November 11, 2011 2:38:24 PM > Subject: HTL Exam > > Hi Joelle, > > > I took the HTL exam in April. I thought I did terribly but ended up > passing (go figure). > > I honestly think it's a lot of memorization. > > I got the flash-cards that compliment the Carson text. Some images were > repeats of the book but most were new. And it was an excellent study > guide for questions like: what is this stain, what's wrong with this > stain/section, what's the best fixative for this stain, etc. I also made > my own flashcards, of which I had probably around 500. Not an easy task. > > I read and high-lighted the Carson and Barncroft text. Then I went > through and made an outline of the text (a loooong outline). > After reviewing that a few times, I made my flashcards. > > Going through the flashcards over the weeks, I could eventually cut out > the ones that I knew the answers to and kept studying the more difficult > ones. > > The Barncroft text was an excellent supplemental for Carson and also had > nice images. > > If you would like my outline, let me know and I will send it over. $500. > Kidding. > > Best of luck! > > Adrienne > > -- > > > > Adrienne Kavanagh HTL (ASCP) > US PATH > 30 W. Century Road > Suite 255 > Paramus NJ 07652 > > -- > > > > Adrienne Kavanagh HTL (ASCP) > US PATH > 30 W. Century Road > Suite 255 > Paramus NJ 07652 From Carol.Freeman <@t> utoledo.edu Mon Nov 14 10:15:19 2011 From: Carol.Freeman <@t> utoledo.edu (Freeman, Carol) Date: Mon Nov 14 10:15:41 2011 Subject: [Histonet] (no subject) Message-ID: We are starting MSI testing in our lab, I am just curious if there is any labs out there doing this on colon biopsy cases. If so we would love some feedback. I would like to know if this is common practice somewhere. From Timothy.Morken <@t> ucsfmedctr.org Mon Nov 14 10:32:25 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Nov 14 10:32:39 2011 Subject: [Histonet] RE: 15 years of Histonet In-Reply-To: References: Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE478@MCINFRWEM003.ucsfmedicalcenter.org> Linda Wrote "By the way, Histonet is coming up on its 16th year anniversary (wow!) and there are currently 3543 members on the list (from >30 countries as of my last tally)." That is really great. I agree with John Kiernan that Histonet really opened up the histology community. Back in the "old" days you were lucky if you knew a few techs outside your own lab, and met a few at the occasional state or national meeting. Most histotechs were very isolated. Since Histonet that has all changed. Now we are all just an email away from getting just about any question answered. I am continually astonished at the breadth of knowledge out there AND the breadth of the field. I think I heard about Histonet from NSH. In any case it was around 1997 and I was working in Saudi Arabia. Histonet was a fantastic resource and I even ended up getting a job back in the US through contacts I made on Histonet (remember that Jeannine?). Since then I peruse it almost daily and occasionally post. In any case it is always interesting to see what the unofficial "topic of the day" is! Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Friday, November 11, 2011 12:01 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unsubscribing from Histonet Dear Histonetters: Hi, I haven't put out many messages on the list recently. I have just been quietly administering the list (and occasionally removing people who ask to be unsubscribed as my time allows). By the way, Histonet is coming up on its 16th year anniversary (wow!) and there are currently 3543 members on the list (from >30 countries as of my last tally). As it keeps coming up, if you wish to unsubscribe or modify your account options, go to this link: http://lists.utsouthwestern.edu/mailman/options/histonet You will need to request your password if you don't know or remember it. I tried to get this link added to the bottom of all the messages but apparently that is not possible with the current software. If you have trouble getting off the list, let me know. If your email address has been changed at your organization, you may have trouble posting messages or unsubscribing. If so, let me know (but provide me with the old address that is on the membership list) and I'll try to help. Thanks, Linda M Histonet administrator Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. 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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Mon Nov 14 10:58:51 2011 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Nov 14 10:58:54 2011 Subject: [Histonet] counterstain for fast red Message-ID: <1321289931.50439.YahooMailNeo@web130107.mail.mud.yahoo.com> Hi Everyone, ? We are currently running some alk phos staining protocols with a fast red chromogen.? My pathologist would like to use something other than Hematoxylin as a counterstain.? What does everyone recommend?? ? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From joelleweaver <@t> hotmail.com Mon Nov 14 11:47:06 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Nov 14 11:47:10 2011 Subject: [Histonet] HT training -competency checklist Message-ID: A couple of people contacted me about a training and competency checklist for intial, entry level HT employees. I put something together that is pretty generic from some items I developed for a previous employer. Of course if I was going to do this for myself, I would expand it out, but it might help those out who are working to develop this instrument for their lab. I will post from my linked in page and if it helps out, great. Just keep in mind that this would not be a copy/paste item and that you might use this as an idea starter only, and would need to add your specific instrumentation processes and procedures, LIS and other SOP's to be incorporated. I still recommend the CAP tool, and also the need to correlate with the job description, and broader organizational objectives, which of course would vary by laboratory.ThanksJoelle Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From rjbuesa <@t> yahoo.com Mon Nov 14 12:14:02 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 14 12:14:06 2011 Subject: [Histonet] Whiskers In-Reply-To: <176C97AAA877D24798ED7376652D0FD815AEE4F263@SRVEXCH02.epistem.local> Message-ID: <1321294442.74073.YahooMailClassic@web65705.mail.ac4.yahoo.com> Us 10% aq.?KOH sol. for half an ahour and process after that. Ren? J. --- On Mon, 11/14/11, Adam Boanas wrote: From: Adam Boanas Subject: [Histonet] Whiskers To: "histonet@lists.utsouthwestern.edu" Date: Monday, November 14, 2011, 10:13 AM Hello, I am currently sectioning individual rat whiskers and follicles and am having some trouble obtaining clean sections from within the centre of the whisker (and intact bulb) without the brittle hair falling out of the block or splintering. Does anyone know of a method that I could use to soften the keratin prior to embedding? I am thinking 10% Sodium Hydroxide or 5% Ammonium Hydroxide but do not really know about appropriate timings for such a small tissue. Many thanks, Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Nov 14 12:15:33 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 14 12:15:36 2011 Subject: [Histonet] using Aquoes mounting media In-Reply-To: <1321284589.35355.YahooMailClassic@web45516.mail.sp1.yahoo.com> Message-ID: <1321294533.99292.YahooMailClassic@web65714.mail.ac4.yahoo.com> For sure the one you bought come with the instructions. In essence your section has to be wet (with water); add the mounting medium and cover. Let it dar. Ren? J. --- On Mon, 11/14/11, Remedy Bi wrote: From: Remedy Bi Subject: [Histonet] using Aquoes mounting media To: histonet@lists.utsouthwestern.edu Date: Monday, November 14, 2011, 10:29 AM Hello friends i wish to fine? out how to use the aqueous mounting media. thanks alot _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Luis.Chiriboga <@t> nyumc.org Mon Nov 14 12:33:36 2011 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Mon Nov 14 12:33:43 2011 Subject: [Histonet] Pan Keratin Mouse Message-ID: Hi All Anyone have any recommendations for a pan cytokeratin that works in mouse tissue? Thanks Luis ------------------------------------------------------------
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From joelleweaver <@t> hotmail.com  Mon Nov 14 12:57:12 2011
From: joelleweaver <@t> hotmail.com (joelle weaver)
Date: Mon Nov 14 12:57:18 2011
Subject: [Histonet] HT training -competency checklist
In-Reply-To: <14E1B84A0B72FC42A84825211D82D16EC754E7A8B7@MMD-DC.molecularmd.local>
References: ,
	<14E1B84A0B72FC42A84825211D82D16EC754E7A8B7@MMD-DC.molecularmd.local>
Message-ID: 


JenniferI got a linked in account and have never paid for it since 2008.I think that you pay only if you want the enhanced page features. I think that the checklist builder is under the lab quality management and competency materials from CAP. I posted the link the other day by entering competency in the search bar. I got to it even though I have not renewed my CAP membership as of yet. My own documents I posted as a word doc from slide share. On my profile, I believe that you can click on the presentations link and then it will show what I have posted at the moment. If none of this works, let me know and if you give me more specifics, perhaps I can help you with your project in a more individualized way? I don't think that the document I posted would be at enough detail to be used by any specific lab anyhow as it is, just trying to help out if someone is struggling to get started since I have worked with this  exact project for awhile.

Joelle Weaver MAOM, BA, (HTL) ASCP
 
http://www.linkedin.com/in/joelleweaver

 > From: jwright@molecularmd.com
> To: joelleweaver@hotmail.com
> Date: Mon, 14 Nov 2011 10:51:11 -0800
> Subject: RE: [Histonet] HT training -competency checklist
> 
> Hi Joelle,
> 
> I tried to click on the CAP website link and I got... Tips & Tricks - Fall 2008 > Share Your Own Tips and Tricks 
>   Share Your Own Tips and Tricks. 
> 
> I also tried to access your linked in page, I even signed up for a linked in account, when I tried to access your page I was prompted to pay a yearly membership fee. 
> 
> Please help, I really don't want to join linked in but I would like to see what you use as training checklist. I promise I will not copy and paste yours and try to pass it off as my own :-)
> 
> Thank-you,
> 
> Jennifer Wright, HTL (ASCP)cm, QIHC
> Senior Histology Technologist
> MolecularMD Corp.
> 1341 SW Custer Drive
> Portland, OR  97219
> Office: 1-503-459-4974 Ext. 158
> Fax: 1-503-459-4976
> jwright@molecularmd.com
> www.molecularmd.com
> 
> 
> This document and any associated attachments may contain information that is 
> privileged, CONFIDENTIAL, and exempt from disclosure under applicable law.  If 
> you are not the intended recipient, please notify me immediately, as the use of
> this information is strictly prohibited.
> 
> 
>  
> 
> 
> 
> 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver
> Sent: 14 November, 2011 9:47 AM
> To: histonet-bounces@lists.utsouthwestern.edu; Histonet
> Subject: [Histonet] HT training -competency checklist
> 
> 
> A couple of people contacted me about a training and competency checklist for intial, entry level HT employees. I put something together that is pretty generic from some items I developed for a previous employer. Of course if I was going to do this for myself, I would expand it out, but it might help those out who are working to develop this instrument for their lab. I will post from my linked in page and if it helps out, great. Just keep in mind that this would not be a copy/paste item and that you might use this as an idea starter only, and would need to add your specific instrumentation processes and procedures, LIS and other SOP's to be incorporated. I still recommend the CAP tool, and also the need to correlate with the job description, and broader organizational objectives, which of course would vary by laboratory.ThanksJoelle
> 
> Joelle Weaver MAOM, BA, (HTL) ASCP
>  
> http://www.linkedin.com/in/joelleweaver
>  		 	   		  _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
 		 	   		  
From Nancy_Schmitt <@t> pa-ucl.com  Mon Nov 14 13:01:20 2011
From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt)
Date: Mon Nov 14 13:01:24 2011
Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 20
In-Reply-To: <20111114180534.BD809450ED@mail.pa-ucl.com>
References: <20111114180534.BD809450ED@mail.pa-ucl.com>
Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B91E1@PEITHA.wad.pa-ucl.com>

Carol-
We started doing the MSI panel on all colon resections earlier this year.  We run this on the BOND system with good results.
Nancy
Dubuque, IA

Message: 5
Date: Mon, 14 Nov 2011 11:15:19 -0500
From: "Freeman, Carol" 
Subject: [Histonet] (no subject)
To: 
Message-ID:
	
Content-Type: text/plain; charset="us-ascii"

 
 
We are starting MSI testing in our lab, I am just curious if there is any labs out there doing this on colon biopsy cases.  If so we would love some feedback.  I would like to know if this is common practice somewhere.




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From madary <@t> verizon.net  Mon Nov 14 14:10:58 2011
From: madary <@t> verizon.net (madary@verizon.net)
Date: Mon Nov 14 14:11:07 2011
Subject: [Histonet] HTL exam
Message-ID: <25364103.3473506.1321301458939.JavaMail.root@vznit170136>


   &nb   that  read   information  needed  to   esp  found  the  B&S and th   was  great  to  read  them  again.If  y   attention  to what you are doing, you will lik   least  get  half  the  answers  from  just being engaged o   Memorization  will  not  help  with  questions that require you to re   cognize what a stain is, an artifact, troubleshooting etc.
   
   Nick(Rocky) Madary, HT/HTL(ASCP)QIHC

   On 11/14/11, histonet-request@lists.utsouthwestern.edu wrote:
   Send Histonet mailing list submissions to
   [1]histonet@lists.utsouthwest   To subscribe or unsubscribe via the World Wide Web, visi   [2]http://lists.utsouthwestern.edu/mailman/li   or, via email, send a message with subject or body '   [3]histonet-request@lists.utsouthwestern.edu
   You can reach the person managing the list at
   histonet-owner@lis   When replying, please edit your Subject li   than "Re: Contents of Histonet digest..."
   <   Today's Topics:
   1. RE: HTL Exam (joelle weaver)
   2. Whisker   3. using Aquoes mounting media (Remedy Bi)
   4. RE: HTL   5. (no subject) (Freeman, Carol)
   6. RE: 15 year   7. counterstain for fast red (Kim Merria   8. HT training -competency checklist (joelle weaver)
   -----   -----------------------------------------------------------------
   Me   Date: Mon, 14 Nov 2011 14:11:19 +0000
   From: joelle weaver &l   Subject: [Histonet] RE: HTL Exam
   To: <[5]aaperghis@uspath.com>, Histonet
   <<   href="mailto:histonet@lists.utsouthwestern.edu"   tar   _djrealurl="mailto:histonet@lists.utsouthwestern.edu">histon   et@lists.utsouthwestern.edu>,
   <[6]histonet-bounces@lis   Message-ID:  Date: Fri, 11 Nov 2011 12:38:24 -0700
   > From: [9]aaperghis@uspath.co   > To: [10]joell   > Subject: HTL Exam
   >
   > Hi Joell   >
   >
   >  I  took the HTL exam in April. I thought I did    up passing (go figure).
   >
   > I honestly thi   >
   >  I got the flash-cards that   were  repeats of the book but most    study  guide  for  questions  like: what is t   with  this  stain/section,  what's the best fixative f   etc.  I  also made my own flashcards, of which I had probably    500. Not an easy task.
   >
   >  I read and high-lighted the   through and made an outline of the    > After reviewing that a few times, I made   >
   >  Going  through  the flashcards over the wee   cut  out  the  ones  that I knew the answers to and kept   more difficult ones.
   >
   >  The  Barncroft  text  was   also had nice images.
   >
   >  If  you would like my outline, let me know and I will send it over.
   $5   >
   > Best of luck!
   >
   > Adrienne
      > --
   >
   >
   >
   > Adrienne Kavanagh HTL    > US PATH
   > 30 W. Century Road
   > Suite 255
      ------------------------------
   Mes   Date: Mon, 14 Nov 2011 15:13:05 +0000
   From: Adam Boanas <<   href="mailto:a.boanas@epistem.co.uk"        target=_blan   _djrealurl="mailto:a.boanas@epistem.co.uk">a.boanas@epistem.co.uk&g   t;
   Subject: [Histonet] Whiskers
   To: "[11]histonet@lists.utsouthwestern.edu   <[12]histonet@lists.utsouthwestern.edu>
   Message-ID:
   <176C97A   AA877D24798ED73[13]76652D0FD815AEE4F263@SRVEXCH02.epistem.loca   Content-Type: text/plain; charset="us-ascii"
   Hello,
   I am currently sectioning individual rat whiskers and follicles and a   m  having some trouble obtaining clean sections from within the centre
   of  th   out of the blo   could  use  to  soften    10% Sodium Hydroxide or 5% Am   about appropriate timings for such    Many thanks,
   Adam
   Adam Boanas
   Senior Resear   Epistem Ltd
   48 Grafton Street
   Manchester, M13 9XX
      ------------------------------
   Message: 3
   Date: Mon,    From: Remedy Bi <[14]birems2@yahoo.com>
   Subject: [Histonet] using A   To: [15]histonet@lists.utsouthwestern.edu
   Message-ID:   <1321284589.35[16]355.YahooMailClassic@web45516   Content-Type: text/plain; charset=us-ascii   Hello  friends  i wish to fine out how to use the aqueous mounting me   dia.
   thanks alot
   ------------------------------
   M   Date: Mon, 14 Nov 2011 15:46:30 +0000
   From: joelle weaver &   Subject: [Histonet] RE: HTL Exam
   To: <[18]aaperghis@uspath.com>,
   <[19]histonet-bounces@lists.utsouthwestern.edu>, Histonet
   <[20]histone   Message-ID:  Date: Mon, 14 Nov 2011 07:08:41 -0700
   > From: [23]aaperghis@uspath.com
   > To: [24]joelleweaver@hotmail.com<   > Subject: Re: HTL Exam
   >
   > I just realized I sent t   the exam.
   >
   > My ap   >
   >
   >
   > ----- Original Message -----
   > From: "Adrienne Aperghis Kavanagh" <[25]aaperghis@uspath.com>
   > To: [26]joelleweaver@hotmail.com
   > Sent:    > Subject: HTL Exam
   >
      >
   >
   >  I  took the HTL exam in April. I t   up
   > passing (go figure).
   >
   > I honestly think it's a lot of memorization.
   >
   > I got t   were
   >  re   study
   >     this
   >   also  made<   500. Not an easy    >
   > I read and high-lighted the Carson and Barncroft tex   > through and made an outline of the text (a loooong o   > After reviewing that a few times, I made my flashcards.
   >
   >  Going  through  the flashcards over the weeks, I could eventua   cut out
   >  the  ones  that  I knew the answers to and kept studying t   difficult
   > ones.
   >
   >  The  Barncroft  text was an    also had
   > nice images.
   >   > If you would like my outline, let me know and I will send it over   $500.
   > Kidding.
   >
   > Best of luck!
   >
   > Ad   >
   > --
   >
   >
   >
   > Adrienne Kava   > US PATH
   > 30 W. Century Road
   > Suite 25   > Paramus NJ 07652
   >
   > --
   >
   >
   > <   > US PATH
   > 30 W. Centur   > Suite 255
   > Paramus NJ 07652
   -----------   Message: 5
   Date: Mon, 14 Nov 2011 11:15:19 -0   From: "Freeman, Carol" <[27]Carol.Freeman@utoledo.edu>
   Subject: [Histonet] (no s   To: <[28]histonet@lists.utsouthwestern.edu>
   Message-ID:
   &   0.utad.utoledo.edu>
   Content-Type: text/plain; charset="us-ascii   We  are  starting MSI testing in our lab, I am just curious    is
   any labs out there doing this on colon biopsy cases. If so w   love some feedback. I would like to know if this is common pract   somewhere.
   ------------------------------
   Message:   Date: Mon, 14 Nov 2011 08:32:25 -0800
   From: "Morken, Timothy" <   Subject: [Histonet] RE: 15 years of Histonet
   To: "'[31]Histonet@lists.utsouthwestern.edu'"
   30 countries as    That is really great. I agree with John Kiernan    opened  up  the histology community. Back in the "old" d   lucky if you knew a few techs outside your own lab, and met a    the  occasional state or national meeting. Most histotechs were very    isolated.  Since Histonet that has all changed. Now we are all just an
   email   continually aston   breadth of the field. <   I  think I heard about Histonet from NSH. In any case it was around 1   997  and  I  was  working  in  Saudi  Arabia. Histonet was a fantastic
   resource  an   contacts I made on H   Since  then  I  peruse it almost da   case  it  is  always interesting to see what   the day" is!
   Tim Morken
   Supervisor, His   UCSF Medical Center
   San Francisco, CA, USA
   --   From:          [33]histonet-bounces@lists.utsouth   [[34]mailto:histonet-bounces@lists.utsouthwestern.edu   Linda Margraf
   Sent: Friday, November 11, 2011 12:01 P   To: '[35]Histonet@lists.utsouthwestern.edu'
   Subject: [Histonet] Unsubscr   Dear Histonetters:
   Hi,  I  haven't  put  out    just  been quietly administering    people  who  ask  to  be unsubscribed as my   Histonet  is coming up on its 16th year anniversa   are  currently 3543 members on the list (from >30 cou   last tally).
   As  it  keeps  coming  up, if you wish to u   account options, go to this link:
   [36]http://lists.utsouthwestern.edu/mailman/options/histo   You will need to request your password if you don't know or    it. I tried to get this link added to the bottom of all the messag   but  apparently that is not possible with the current software. If you
   ha   address  has bee   posting  messages  or un   me with the old address that i   to help.
   Thanks,
   Linda M
   Hi   Please consider the environment before printing   This  e-mail,  facsimile,  or  letter  and  any files or atta   transmitted with it contains
   information that is confidential an   intended only for the use of the
   indiv   the intended r   disclosures  are prohibited without proper authorizatio   not the intended recipient, any
   disclosure,  copying,  print   strictly prohibited and possibly a
   vi   received this    please  notify  Children's  Medical  Center  Dallas  imm   214-456-4444 or via e-mail at
   privacy@childrens.com.  Childre   affiliates hereby claim all
   applicable
disclosu not the intende disclosure, copying, printing, or use of t strictly prohibited and possibly a
viola received this inf please notify Children's Medical Center 214-456-4444 or via e-mail at
privacy affiliates hereby applicable privileges related to this information
________ Histonet mailing list [37]Histonet@lis [38]http://lists.utsou ------------ Message: 7 Date: Mon, 14 Nov 2011 08:58:51 -08 From: Kim Merriam <[39]kmerriam2003@yahoo.com> Subject: [Histonet] counterstain f To: Histonet <[40]histonet@lists.utsouthwestern.edu> Mes <132128993[41]1.50439.YahooMailNeo@we Content-Type: text/plain; charset=i Hi Everyone, We are currently running some al fast red chromogen. My pathologist w other than Hematoxylin as a counterstain. recommend? Thanks, Kim Kim Cambridge, MA ------------------------- Message: 8 Date: Mon, 14 Nov 2011 17:47:06 +0000 From: j Subject: [Histonet] HT training -competency c To: <[43]histonet-bounces@lists.utsouthwestern.edu>, Histonet <[44]histonet@lists.utsouthwestern.edu> Message-ID: Content-Type: text/plain; chars A couple of people contacted me about a traini checklist for intial, entry level HT employees. I put som together that is pretty generic from some items I developed for a pr evious employer. Of course if I was going to do this for myself, I would ex develop this in page and if it helps not be a copy/paste item and starter only, and would need to add your processes and procedures, LIS and other SOP's to still recommend the CAP tool, and also the need to corre the job description, and broader organizational objectives, which course would vary by laboratory.ThanksJoelle Joelle Weaver MAOM, [46]http://www.linkedin.com/in/joelleweaver ----- __________________________________________ Histonet mailing list [47]Histonet@lists.utsouthwestern.edu [48]http://lists.utsouthwestern.edu/mailman/listinfo/his End of Histonet Digest, Vol 96, Issue 20 ************** References 1. 3D"mailto:histonet@lists.utsouthwestern.edu" 2. 3D"http://lists.utsouthwestern.edu/mailman/ 3. 3D"mailto:histonet-request@lists.ut 4. 3D"mailto:joelleweaver@hotmail.com" 5. 3D"mailto:aaperghis@uspath.com" 6. file://localhost/tmp/3D" 7. 3D"mailto:080A40866F1DB35D8C00@phx.gbl" 8. 3D"http://www.linkedin.com/in/joelleweaver" 9. 3D"mailto:aaperghis@uspath.com" 10. 3D"mailto:joelleweaver@hotmai 11. file://localhost/tmp/3D"m 12. 3D"mailto:histonet@lists.utsouthwester 13. 3D"mailto:76652D0FD815AEE4F263@S 14. 3D"mailto:birems2@yahoo.com" 15. 3D"mailto:histonet@ 16. 3D"mailto:355.YahooMailCla 17. 3D"mailto:joelleweaver@hotmail.com" 18. 3D"mailto:aaperghis@uspath.com" 19. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 20. 3D"mailto:histonet@lists.utsouthwestern.edu" 21. 3D"mailto:05A245376989531D8C00@phx.gbl" 22. file://localhost/tmp/3D"http 23. 3D"mailto:aaperghis@uspath.com" 24. 3D"mailto:joelleweaver@hotmail.com" 25. file://localhost/tmp/3D 26. 3D"mailto:joelleweaver@hotmail.com" 27. 3D"mailto:Car 28. 3D"mailto:histonet@lists.uts 29. 3D"mailto:607F5EEDF31101 30. 3D"mailto:Timothy.Morken@ucsfmedctr.org" 31. 3D"mailto:Histonet@lists.utsouthwestern. 32. 3D"mailto:B21122072BF89F5EE478@MCINFRWEM003.ucsfmedicalcenter.org" 33. 3D"mailto:his 34. 3D"mailto:histonet-bounces@list 35. 3D"mailto:Histonet@lists.utsouthweste 36. 3D"http://lists.utsouthwestern.edu/mailman/options/h 37. 3D"mailto:Histonet@lists.utsouthwestern.edu" 38. 3D"http://lists.uts=/ 39. 3D"mailto:kme 40. 3D"mailto:histo 41. 3D"mailto:1.50439.Yaho 42. 3D"mailto:joelleweaver@hotmail 43. 3D"mailto:histonet-bounces@ 44. 3D"mailto:histonet@lists 45. 3D"mailto:E15BAF2AC5761F9D8C 46. 3D"http://www.linkedin.co=/ 47. 3D"mailto:His 48. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/h From madary <@t> verizon.net Mon Nov 14 14:12:51 2011 From: madary <@t> verizon.net (madary@verizon.net) Date: Mon Nov 14 14:12:55 2011 Subject: [Histonet] rat whiskers Message-ID: <2961933.3473747.1321301571471.JavaMail.root@vznit170136> &nb follicles on aga agar/paraffin block with a new should be okay. You can also try and cut practice this several times first. &nb Nick(Rocky) Madary, HT/HTL On 11/14/11, histonet-request@lists.utsouthw Send Histonet mailing list submissions to [1]histonet@lis To subscribe or unsubscribe via the World [2]http://lists.utsouthwestern. or, via email, send a message with sub [3]histonet-request@lists.utsouthwestern. You can reach the person managing the list at [4]his When replying, please edit than "Re: Contents of Histonet Today's Topics: 1. RE: HTL Exam (joelle weaver 2. Whiskers (Adam Boanas) 3. using Aquoes mounting media (Remedy Bi 4. RE: HTL Exam (joelle weaver) 5. (no subject) (Freeman, Carol) 6. RE: 15 years of Histonet (Morken, Timothy) 7. counterstain for fast 8. HT training -competency checklist (joelle weaver) ----------------------------------------------------------------- ----- Message: 1 Date: Mon, 14 Nov 2011 14:11:19 +0000 From: j Subject: [Histonet] RE: HTL Exam To: << target=_blank _djrealurl="mailto:aaperghis@uspath.com">aaperghis@uspath.com>, Hi stonet <[6]histonet@lists.utsouthwestern.edu>, <[7]histo Message-ID: Message-ID: <176C97AAA877D24798ED73[15]76652D0FD815AEE4F263@SRVEXCH 02.epistem.local> Content-Type: text/plain; charset="us-ascii"< Hello, I am currently sectioning individual rat whiskers and am having some trouble obtaining clean sections from within t centre of the whisker (and intact bulb) without the brittle hair falling that I could thinking 10% Sodium Hyd really know about appropriate ti Many thanks, Adam Adam BoanasSenior Research Associate Epistem Ltd 48 Grafton Street Manchest ------------------------------ Message: 3 Date: Mon, 14 Nov 2011 07:29:49 -0800 (PST) From: Remedy Bi <[16]birems2@yahoo.com> Subject: [Hi To: [17]histonet@lists.utsouthwestern.edu Message-ID: <1321284589.35[18]355.YahooMailC Content-Type: text/plain; cha Hello friends i wish to fine out how to use the aque media. thanks alot ------------------------ Message: 4 Date: Mon, 14 Nov 2011 15:46:30 +0000 From: Subject: [Histonet] RE: HTL Exam To: < [20]aaperghis@uspath.com>,<[21]histonet-bounces@lists.utsouthwestern.e du>, Histone <[22]histonet@lists.utsouthwestern.edu> Message-ID: > To: [27]joelleweaver@hotmail.com > Sent: Friday, November 11, 2011 2:38:24 PM > Subject: HTL Exam< > Hi Joelle, > > > I took the HTL exam i up > passing (go figure). > > I honestly think it's a lot of memorization. > wer > repeats of the book but most were new. And it was an excellent st udy > guide for questions like: what is this stain, what's wrong with this > stain/section, what's the best fixative for this stain, etc. also made > my own flashcards, of which I had probably around 500. task. > > I read and high-lighted the Carson and B > through and made an outline of the text > After reviewing that a few times, I made my fl > > Going through the flashcards over the weeks, I c cut out > the ones that I knew the answers to and kep difficult > ones. > > The Barncroft also had > nice ima > > If you would like my outline, let me know and I will $500. > Kidding. > > Best of luck! > > Adrienne > > -- > > > > A > US PATH > 30 W. Century Road & > Paramus NJ 07652 > > -- > > > > Adrienne Kavanagh HTL (ASCP) > US PATH > > Suite 255 > Paramus NJ 07652 ------------------------------ Message: 5 Date: Mon, 14 Nov 2011 From: "Freeman, Carol" <[28]Carol.Freeman@utoledo.edu> Subject: [H To: <[29]histonet@lists.utsouthwestern.edu> Me Content-Type: text/plain; chars We are starting MSI testing in our lab, I am any labs out there doing this on colon biopsy love some feedback. I would like to know if this i somewhere. ------------------------------ Message: 6 Date: Mon, 14 Nov 2011 08:32:25 -0800 From: "Morken, Subject: [Histonet] RE: 15 years To: "'[32]Histonet@lists.utsouthwestern.edu'" <[33]Histonet@lists.ut Message-ID: <8D7C2D242DBD45498006[34]B21122072BF89F5EE478@MCINFRWEM003.ucsfmedica lcenter.org> Content-Type: text/plain; charset=us-ascii Linda Wrote "By the way, Histonet is coming up on its 16th year anniver (from >30 c That is really great. I agree with J opened up the histology community. Back in lucky if you knew a few techs outside your own lab the occasional state or national meeting. Most histotech isolated. Since Histonet that has all changed. Now we are all j an email away from getting just about any question answered. I am conti breadth of I think I heard about Histonet from NSH. In any case it 1997 and I was working in Saudi Arabia. Histonet was a fantastic resource and I even ended up getting a job back in the US through contacts Since then I peruse case it is always interesting the day" is! Tim Morken Sup UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: [35]histonet-bounces [[36]mailto:histonet-bounces@lists.utso Linda Margraf Sent: Friday, November 11 To: '[37]Histonet@lists.utsouthwestern.edu' Subject: [Hist Dear Histonetters: Hi, I ha just been quietly people who ask to be unsu Histonet is coming up on its 16th are currently 3543 members on the list (f last tally). As it keeps coming up, if account options, go to this link: [38]http://lists.utsouthwestern.edu/mailman You will need to request your password if you it. I tried to get this link added to the bottom of but apparently that is not possible with the current softw you have trouble getting off the list, let me know. If your email a ddress has been changed at your organization, you may have trouble posting me with the old to help. Thanks, Linda M Histonet administrator Please consider the environment b This e-mail, facsimile, or letter and any transmitted with it contains information that is c intended only for the use o individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without prope not the intended recipient, any disclosure, strictly prohibited and po violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Cen 214-456-4444 or via e-mail at privacy@children affiliates hereby claim all applicable privileges related to this information. environment be
& and inform intended onl individual(s) and entity(ies) to whom the intended recipient, further
disclosures are prohibited without proper authorization. If you are no disclosure, copying, printing strictly prohibited and possibly a
please notify Children's Me 214-456-4444 or via e-mail at
applicable privileges related to thi
_______________________________________________ Histonet mailing li [39]Histonet@lists.utsouthwestern.edu [40]http: ------------------------------ Message: 7 Date: Mon, 14 Nov 2011 From: Kim Merriam <[41]kmerriam2003@yahoo.com> Subject: [Histonet] To: Histonet <[42]histonet@lists.utsouthwestern.ed Message-ID: <132128993[43]1.50439 Content-Type: text/pl Hi Everyone, We are currently red chromogen. than Hematoxylin as a coun Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA --------- Message: 8 Date: Mon, 14 Nov 2011 17:47:06 From: joelle weaver <[44]joelleweaver@hotmail.com> Subject: [Histonet] HT traini To: <[45]histonet-bounces@lists.utsou <[46]histonet@lists.utsouthwestern.edu> Message-ID: Content-Type: t A couple of people contacted m checklist for intial, entry level HT empl together that is pretty generic from some items I de previous employer. Of course if I was going to do this for my would expand it out, but it might help those out who are working to develop this instrument for their lab. I will post from my linked in page not be a copy starter only, and would processes and procedures, LIS and still recommend the CAP tool, and also t the job description, and broader organizational o course would vary by laboratory.ThanksJoelle Joe [48]http://www.linkedin.com/in/joelleweaver< ------------------------------ __________________________ Histonet mailing list Histonet@lists.utsouthwestern [49]http://lists.utsouthwestern.edu/mai End of Histonet Digest, Vol 96, Issue 20< BR>**************************************** References 1. 3D"mailto:histonet@lists.utsouthwestern.edu" 2. 3D"http://lists.utsouthwester=/ 3. 3D"mailto:histonet-re 4. 3D"mailto:histonet-owner@lists.utsouthwestern.edu" 5. 3D"mailto:joelleweaver@hotmail 6. 3D"mailto:histonet@lists.utsouthw 7. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 8. 3D"mailto:080A40866F1DB35D8C00@phx.gbl" 9. 3D"http://www.linkedin.com/in/joelleweaver" 10. 3D"mailto:aaperghis@ 11. 3D"mailto:joel 12. 3D"mailto:a.boanas@epistem.co.uk" 13. 3D"mailto:histonet@lists.utsouthwestern.edu" 14. 3D"mailto:histonet@list 15. 3D"mailto:76652D0 16. 3D"mailto:birems2@yahoo.com" 17. file://localhost/tmp/3D"m 18. 3D"mailto:3 19. 3D"mailto:joelleweaver@hotmai 20. 3D"mailto:aaperghis@uspath.com" 21. 3D"mailto:histonet-bounces@lists.utsouthw 22. 3D"mailto:histonet@lists.utsouthwester 23. 3D"mailto:05A245376989531D8C00@phx.gbl" 24. 3D"mailto:aaperghis@uspath.com" 25. 3D"mailto:joelleweaver@hotmail.com" 26. 3D"mailto:aaperghis@uspath.com" 27. 3D"mailto:joelleweaver@hotmail.com" 28. ="mailto:Carol.Freeman@utoledo.edu" 29. 3D"mailto:hist 30. 3D"mailto: 31. 3D"mailto:Timothy.Morken@ucsfmedc 32. 3D"mailto:Histonet@lists.u 33. 3D"mailto:Histonet@lists.utsouthwestern.edu" 34. 3D"mailto:B21122072BF89F5EE478@MCINFRWEM003.ucsfmedical 35. ="mailto:histonet-bounces@lists.utsouthwestern.edu" 36. 3D"mailto:histone 37. 3D"mailto:Histonet@list 38. 3D"http://lists.utsouthwestern.edu/mai 39. 3D"mailto:Histonet@lists.utsouthwestern.e 40. file://localhost/tmp/3D"htt 41. ="mailto:kmerriam2003@yahoo.com" 42. ="mailto:histonet@lists.utsouthwestern.edu" 43. file://localhost/tmp/3D"mai 44. 3D"mailto:joel 45. 3D"mailto:h 46. file://localhost/tmp/3D"mailt 47. 3D"mailto:E1 48. 3D"http:/ 49. 3D"http://lists.utsouthwestern.edu/m From foreightl <@t> gmail.com Mon Nov 14 15:47:22 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Mon Nov 14 15:47:28 2011 Subject: [Histonet] Second Shift lead position available at CellNetix Labs in Seattle Message-ID: Histonet, *Job Title:* Lead Histotech ? 2nd shift *Department: * Laboratory *Reports To:* Histology Supervisor *Shifts: *3:00pm to 11:30PM Monday thru Friday *SUMMARY* Under the supervision of the Histology Supervisor, the Lead Histology Technician/Technologist, performs with proficiency and understanding, the tests and procedures assigned to the Histology Department. The position is responsible for grossing, processing, embedding, cutting and staining of tissue blocks, accurate reporting of results, quality control, teaching, special projects, equipment maintenance and all other duties assigned by the Supervisor. * * *ESSENTIAL FUNCTIONS* ? Performs both routine and complex special procedures with the understanding of tissue structures, techniques, principles, theory and instrumentation. ? Able to gross section and dictate descriptions of simple specimens, process, embed, cut and stain all types of tissue specimens received in the Histology Department. ? Coverslip stained sections, verify that the staining shows adequate cellular distinction, label, and distribute to the pathologist responsible for reviewing the case. ? Assures specimen labeling requirements are met and necessary clinical information is available as needed. ? Assist pathologists with frozen section staining and processing. ? Assists laboratory assistant or performs accessioning of patient specimens into laboratory computer system when needed. ? Demonstrates general knowledge of pathological and physiological conditions that affect test results and tissue staining. ? Selects and maintains appropriate tissue blocks to serve as controls for Special Satins. Maintains all documentation that insures that all Special Stain control blocks have been adequately tested. Cuts and labels control slides for Histology, Special Stains and IHC. ? Able to process and decal bone specimens. ? Recognize and troubleshoot both routine and complex problems and assist other Histotechs and lab assistants with technical problems. ? Monitor quality control results and take immediate and proper action when controls are unacceptable. Follow defined procedures with only supervisor or pathologist approved modifications or deviations. ? Complete all instrument function verification, maintenance, and documents according to procedure in Histology area. Ensure that equipment defects and malfunctions are reported and repaired. ? Maintains a neat, clean, and orderly work area. At the end of the day?s work; is responsible for the cleaning of his/her microtome, filing his/ her cut blocks and cleaning any soiled glassware. ? Responsible for the proper handling and disposal of all biohazardous materials and chemically hazardous materials including the neutralization or recycling of chemicals before disposal or reuse. ? Cuts slides for IHC testing or other sendouts in conformance with procedures and submits them for analysis. ? Takes on additional responsibility for training Laboratory personnel including Laboratory Assistants, NRT?s and other Histotechs in Histology procedures. ? Maybe be asked to write/ update Operating Procedures. ? Must be able to express himself/ herself effectively both in written and verbal communications. ? Assists supervisory staff in monitoring workflow and insuring that work/ staining priorities are met for the department. ? Will be asked to put new tests/ stains on line. ? May assist pathologists or physicians in the collection of specimens from patients. ? Participates in special projects or other duties as assigned by supervisor staff or company. ? Always maintains a pleasant, courteous attitude when answering the telephone. ? Conforms to laboratory timekeeping procedures. Keeps unexcused absences and late arrivals to a minimum. ? Participates in weekend and holiday schedules as staffing requirements dictate. Remains flexible and works a share of overtime or different shifts if necessary during staffing shortages or emergencies. ? Participates in continuing education classes and courses. Strongly encouraged to keep updated on recent advances in the field of histology and to take at least 10 credit hours of continuing education a year. ? Highly encouraged to maintain membership in a professional Histology organization. ? Always maintains a safe work environment and attends all safety training classes and conforms to all company safety guidelines and requirements. ? Conforms to established and new procedures and policies instituted by the company. ? Conforms to the Laboratory Dress code and wears appropriate name badge at all times. ? Able to accept guidance and constructive criticism. Displays a mature and professional attitude, is honest, and has a strong sense of responsibility toward patients and clients. ? Able to do all the duties of a HT, Non ? Registered Histotechnician, Lab Tech II, and Laboratory Assistant within the Histology department. During heavy workloads or staffing shortages, the Histology Technologist may be required to perform any or all of the duties in these positions. We are seeking an experienced person for a lead position on 2nd shift with hours from 3pm to 11:30pm. This is a hands-on position with the Lead performing all histo duties in addition to being a trainer and answer person for the shift. Five years histotech experience required with at least 1 year in a Lead or higher role. Must be certified HT/HTL. Please apply via the CellNetix website: http://www.cellnetix.com/careers/job-openings Thanks, -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From jnocito <@t> satx.rr.com Mon Nov 14 16:36:29 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Nov 14 16:36:49 2011 Subject: [Histonet] RE: 15 years of Histonet In-Reply-To: <8D7C2D242DBD45498006B21122072BF89F5EE478@MCINFRWEM003.ucsfmedicalcenter.org> References: <8D7C2D242DBD45498006B21122072BF89F5EE478@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: Can't agree more. I mean, where would Joe the Toe be without the Histonet? By the way, just read the reviews from the lecture my buddy Hector and I gave in Cinci this year. One of the critiques stated that Hector and I are the biggest egomaniacs this side of space. Wow, I've been called a lot of things in my life, but an egomaniac? Really? That hurt, right to the core. JTT ----- Original Message ----- From: "Morken, Timothy" To: Sent: Monday, November 14, 2011 10:32 AM Subject: [Histonet] RE: 15 years of Histonet Linda Wrote "By the way, Histonet is coming up on its 16th year anniversary (wow!) and there are currently 3543 members on the list (from >30 countries as of my last tally)." That is really great. I agree with John Kiernan that Histonet really opened up the histology community. Back in the "old" days you were lucky if you knew a few techs outside your own lab, and met a few at the occasional state or national meeting. Most histotechs were very isolated. Since Histonet that has all changed. Now we are all just an email away from getting just about any question answered. I am continually astonished at the breadth of knowledge out there AND the breadth of the field. I think I heard about Histonet from NSH. In any case it was around 1997 and I was working in Saudi Arabia. Histonet was a fantastic resource and I even ended up getting a job back in the US through contacts I made on Histonet (remember that Jeannine?). Since then I peruse it almost daily and occasionally post. In any case it is always interesting to see what the unofficial "topic of the day" is! Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Friday, November 11, 2011 12:01 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unsubscribing from Histonet Dear Histonetters: Hi, I haven't put out many messages on the list recently. I have just been quietly administering the list (and occasionally removing people who ask to be unsubscribed as my time allows). By the way, Histonet is coming up on its 16th year anniversary (wow!) and there are currently 3543 members on the list (from >30 countries as of my last tally). As it keeps coming up, if you wish to unsubscribe or modify your account options, go to this link: http://lists.utsouthwestern.edu/mailman/options/histonet You will need to request your password if you don't know or remember it. I tried to get this link added to the bottom of all the messages but apparently that is not possible with the current software. If you have trouble getting off the list, let me know. If your email address has been changed at your organization, you may have trouble posting messages or unsubscribing. If so, let me know (but provide me with the old address that is on the membership list) and I'll try to help. Thanks, Linda M Histonet administrator Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail

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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Mon Nov 14 17:12:26 2011 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Nov 14 17:12:37 2011 Subject: [Histonet] counterstain for fast red In-Reply-To: <1321289931.50439.YahooMailNeo@web130107.mail.mud.yahoo.com> References: <1321289931.50439.YahooMailNeo@web130107.mail.mud.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A04E74@xmdb02.nch.kids> Try 1-2% Ethyl green in acetate buffer (pH4) - will give you green nuclei (of course) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Tuesday, 15 November 2011 3:59 AM To: Histonet Subject: [Histonet] counterstain for fast red Hi Everyone, ? We are currently running some alk phos staining protocols with a fast red chromogen.? My pathologist would like to use something other than Hematoxylin as a counterstain.? What does everyone recommend?? ? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From joelleweaver <@t> hotmail.com Mon Nov 14 17:54:17 2011 From: joelleweaver <@t> hotmail.com (joelle weaver ) Date: Mon Nov 14 17:54:21 2011 Subject: [Histonet] ? QIHC Message-ID: Contemplating the IHC qualification and wondering if supervisors/managers can provide feedback as to their feeling/perception of this credential, how you feel it might effect/limit hiring for you when you review candidates? Want to make sure that I am investing in something that will truly aid my marketability. Thanks Sent from my Verizon Wireless BlackBerry From ccrowder <@t> vetmed.lsu.edu Mon Nov 14 18:20:35 2011 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Mon Nov 14 18:23:58 2011 Subject: [Histonet] EM question Message-ID: I am the first to admit I know little to nothing about EM. I have a researcher who read an article today that said in the method that they had taken cells, applied them to copper EM grids and immersed them in liquid nitrogen to fix them. Has anyone ever heard of this technique? and if you have, do you have any directions or references for this technique? Cheryl From jemahiser <@t> gmail.com Mon Nov 14 18:38:18 2011 From: jemahiser <@t> gmail.com (J Emahiser) Date: Mon Nov 14 18:38:42 2011 Subject: [Histonet] Dried Tissue Marking Ink Message-ID: I've been having issues with some of my tissue marking ink drying up. Is there any solvent I can add to reconstitute it, or do I just need to get new bottles? -- J Emahiser Mohs Tech From Nancy_Schmitt <@t> pa-ucl.com Tue Nov 15 07:39:59 2011 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue Nov 15 07:40:10 2011 Subject: [Histonet] Lithium carbonate Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B928C@PEITHA.wad.pa-ucl.com> Good Morning- Making up some lithium carbonate - it is staying very milky in appearance - have not previously had that happen. We tried a couple different times - same result. We are not doing anything different - 5g lithium carbonate to 500ml Type I water. Any thoughts? Thanks, Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Dana.Spencer <@t> PCMH.COM Tue Nov 15 08:17:06 2011 From: Dana.Spencer <@t> PCMH.COM (Dana Spencer) Date: Tue Nov 15 08:17:46 2011 Subject: [Histonet] Slide and Cassette printing Message-ID: <4EC22E11.536C.000A.0@PCMH.COM> What hardware are you using for printing slides and cassettes? There are many good systems out there and each have their flaws, but specifically I have heard negative rumblings about the PrintMate and SlideMate Systems from ThermoFisher. Are you using labels at the microtome? I would welcome any feedback or recommendations esp from larger institutions with large volumes. Thanks, Dana Spencer, CT(ASCP) Anatomic Pathology Manager Pitt County Memorial Hospital Greenville, NC 27834 ------------------------------------------------------------------------------ The contents of this e-mail (and any attachments) are confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. ============================================================================== From koellingr <@t> comcast.net Tue Nov 15 08:19:23 2011 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Nov 15 08:19:40 2011 Subject: [Histonet] EM question In-Reply-To: Message-ID: <331082704.1898578.1321366763290.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Cheryl, ? Have done?some things?with ordinary sectioning?cryo-electron microscopy but not your specific application.? But can go to Cold Harbor Springs Protocols and they can get "Immersion Freezing of Cell Monolayers for Cryo-Electron Tomongraphy". ? Even better (more immediate) go here http://www.jove.com/video/1943/electron-cryotomography-of-bacterial-cells ? and is a California Institute of Technology (really well produced) video/audio/written explaination of the whole procedure. ? Ray ? Ray Koelling PhenoPath Labs Seattle, WA ----- Original Message ----- From: "Cheryl Crowder" To: histonet@lists.utsouthwestern.edu Sent: Monday, November 14, 2011 4:20:35 PM Subject: [Histonet] EM question I am the first to admit I know little to nothing about EM. ?I have a researcher who read an article today that said in the method that they had taken cells, applied them to copper EM grids and immersed them in liquid nitrogen to fix them. ?Has anyone ever heard of this technique? and if you have, do you have any directions or references for this technique? Cheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From silvinamolinuevo <@t> yahoo.com.ar Tue Nov 15 08:57:20 2011 From: silvinamolinuevo <@t> yahoo.com.ar (Silvina Molinuevo) Date: Tue Nov 15 09:00:55 2011 Subject: [Histonet] Re:counterstain for fast red Message-ID: <1321369040.38494.YahooMailNeo@web113603.mail.gq1.yahoo.com> Hi Kim! light green ussually looks well with red background. before you use it be sure to know how to prepare it! best regards, sil From loftonjt <@t> holycrosshealth.org Tue Nov 15 09:22:08 2011 From: loftonjt <@t> holycrosshealth.org (Jimmy Lofton) Date: Tue Nov 15 09:22:23 2011 Subject: [Histonet] Processor list of cases for Competency Message-ID: <4EC23D500200005600003F6A@nodcdmg2.no.trinity-health.org> Does anyone in histoland have a list of types of specimens that a histotechnologist who is processing specimens could be used for initial competency. Thanks, Jimmy Jimmy Lofton, M.S., HT,CT(ASCP) Manager Histology Laboratory Holy Cross Hospital 1500 Forest Glen Road Silver Spring, MD 20910-1484 301-754-7353 (Phone) 301-754-8563 (Fax) loftonjt@holycrosshealth.org Trinity Health MailGate made the following annotations --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and privileged under state and Federal privacy laws. If you received this e-mail in error, be aware that any unauthorized use; disclosure, copying, or distribution is strictly prohibited. Please contact the sender immediately and destroy all copies of this message. --------------------------------------------------------------------- From mamawooo <@t> hotmail.com Tue Nov 15 11:02:16 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Tue Nov 15 11:02:25 2011 Subject: [Histonet] Slide and Cassette printing In-Reply-To: <4EC22E11.536C.000A.0@PCMH.COM> References: <4EC22E11.536C.000A.0@PCMH.COM> Message-ID: Hi Dana, We used the slide labels for the Vantage system without any problems for several years. We labeled at the cutting station and NEVER had a label wash off or smear in about three years. I highly recommend it. You will also see a huge gain in productivity elimination double labeling. Hope all is well with you. Call me anytime, it would be great to hear form you. Jan Mahoney Omaha, NE > Date: Tue, 15 Nov 2011 09:17:06 -0500 > From: Dana.Spencer@PCMH.COM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide and Cassette printing > > What hardware are you using for printing slides and cassettes? There are many good systems out there and each have their flaws, but specifically I have heard negative rumblings about the PrintMate and SlideMate Systems from ThermoFisher. Are you using labels at the microtome? I would welcome any feedback or recommendations esp from larger institutions with large volumes. > > Thanks, > Dana Spencer, CT(ASCP) > Anatomic Pathology Manager > Pitt County Memorial Hospital > Greenville, NC 27834 > > ------------------------------------------------------------------------------ > The contents of this e-mail (and any attachments) are confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. > ============================================================================== > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin <@t> mhhcc.org Tue Nov 15 11:33:16 2011 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Tue Nov 15 11:33:25 2011 Subject: [Histonet] ?QIHC Message-ID: I WOULD LIKE TO KNOW ALSO Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. From wbenton <@t> cua.md Tue Nov 15 11:44:52 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Tue Nov 15 11:45:02 2011 Subject: [Histonet] ?QIHC In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD9206C20444F@CUAEXH1.GCU-MD.local> I think it is valuable since it allows you to learn things through study and application that you may not have been previously exposed to while preparing for the test. The test is strictly theory now versus the first year when stained slides were submitted and there was zero theory. Given the rapid changes in histology with ISH, IHC, IFs and other molecular based testing I don't see how it could hurt you to earn this credential. I'm not sure how pays is differentiated in the market for this qualification, but I do know that I see more and more jobs, especially those for IHC that require it or it is highly desirable for the position. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org [sbaldwin@mhhcc.org] Sent: Tuesday, November 15, 2011 12:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ?QIHC I WOULD LIKE TO KNOW ALSO Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From foreightl <@t> gmail.com Tue Nov 15 11:51:01 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Nov 15 11:51:10 2011 Subject: [Histonet] Second Shift lead position available at CellNetix Labs in Seattle In-Reply-To: References: Message-ID: Histonet, *Job Title:* Lead Histotech ? 2nd shift *Department: * Laboratory *Reports To:* Histology Supervisor *Shifts: *3:00pm to 11:30PM Monday thru Friday *SUMMARY* Under the supervision of the Histology Supervisor, the Lead Histology Technician/Technologist, performs with proficiency and understanding, the tests and procedures assigned to the Histology Department. The position is responsible for grossing, processing, embedding, cutting and staining of tissue blocks, accurate reporting of results, quality control, teaching, special projects, equipment maintenance and all other duties assigned by the Supervisor. * * *ESSENTIAL FUNCTIONS* ? Performs both routine and complex special procedures with the understanding of tissue structures, techniques, principles, theory and instrumentation. ? Able to gross section and dictate descriptions of simple specimens, process, embed, cut and stain all types of tissue specimens received in the Histology Department. ? Coverslip stained sections, verify that the staining shows adequate cellular distinction, label, and distribute to the pathologist responsible for reviewing the case. ? Assures specimen labeling requirements are met and necessary clinical information is available as needed. ? Assist pathologists with frozen section staining and processing. ? Assists laboratory assistant or performs accessioning of patient specimens into laboratory computer system when needed. ? Demonstrates general knowledge of pathological and physiological conditions that affect test results and tissue staining. ? Selects and maintains appropriate tissue blocks to serve as controls for Special Satins. Maintains all documentation that insures that all Special Stain control blocks have been adequately tested. Cuts and labels control slides for Histology, Special Stains and IHC. ? Able to process and decal bone specimens. ? Recognize and troubleshoot both routine and complex problems and assist other Histotechs and lab assistants with technical problems. ? Monitor quality control results and take immediate and proper action when controls are unacceptable. Follow defined procedures with only supervisor or pathologist approved modifications or deviations. ? Complete all instrument function verification, maintenance, and documents according to procedure in Histology area. Ensure that equipment defects and malfunctions are reported and repaired. ? Maintains a neat, clean, and orderly work area. At the end of the day?s work; is responsible for the cleaning of his/her microtome, filing his/ her cut blocks and cleaning any soiled glassware. ? Responsible for the proper handling and disposal of all biohazardous materials and chemically hazardous materials including the neutralization or recycling of chemicals before disposal or reuse. ? Cuts slides for IHC testing or other sendouts in conformance with procedures and submits them for analysis. ? Takes on additional responsibility for training Laboratory personnel including Laboratory Assistants, NRT?s and other Histotechs in Histology procedures. ? Maybe be asked to write/ update Operating Procedures. ? Must be able to express himself/ herself effectively both in written and verbal communications. ? Assists supervisory staff in monitoring workflow and insuring that work/ staining priorities are met for the department. ? Will be asked to put new tests/ stains on line. ? May assist pathologists or physicians in the collection of specimens from patients. ? Participates in special projects or other duties as assigned by supervisor staff or company. ? Always maintains a pleasant, courteous attitude when answering the telephone. ? Conforms to laboratory timekeeping procedures. Keeps unexcused absences and late arrivals to a minimum. ? Participates in weekend and holiday schedules as staffing requirements dictate. Remains flexible and works a share of overtime or different shifts if necessary during staffing shortages or emergencies. ? Participates in continuing education classes and courses. Strongly encouraged to keep updated on recent advances in the field of histology and to take at least 10 credit hours of continuing education a year. ? Highly encouraged to maintain membership in a professional Histology organization. ? Always maintains a safe work environment and attends all safety training classes and conforms to all company safety guidelines and requirements. ? Conforms to established and new procedures and policies instituted by the company. ? Conforms to the Laboratory Dress code and wears appropriate name badge at all times. ? Able to accept guidance and constructive criticism. Displays a mature and professional attitude, is honest, and has a strong sense of responsibility toward patients and clients. ? Able to do all the duties of a HT, Non ? Registered Histotechnician, Lab Tech II, and Laboratory Assistant within the Histology department. During heavy workloads or staffing shortages, the Histology Technologist may be required to perform any or all of the duties in these positions. We are seeking an experienced person for a lead position on 2nd shift with hours from 3pm to 11:30pm. This is a hands-on position with the Lead performing all histo duties in addition to being a trainer and answer person for the shift. Five years histotech experience required with at least 1 year in a Lead or higher role. Must be certified HT/HTL. Please apply via the CellNetix website: http://www.cellnetix.com/careers/job-openings Thanks, -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From cpyse <@t> x-celllab.com Tue Nov 15 12:05:19 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Nov 15 12:05:28 2011 Subject: [Histonet] HPV high & low risk Message-ID: <002e01cca3c1$22d594e0$6880bea0$@com> Hi Histonetters I am looking to add to our test menu the High and low risk HPV. What is everyone using out there. Instrumentation? Vendor? Pros and cons to any system. I would appreciate any input. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manger X-Cell Laboratories e-mail cpyse@x-celllab.com From tajibade <@t> echd.org Tue Nov 15 12:07:24 2011 From: tajibade <@t> echd.org (Tunde Ajibade) Date: Tue Nov 15 12:07:37 2011 Subject: [Histonet] ?QIHC In-Reply-To: References: Message-ID: Study for this certification gives you broad knowledge in all aspects of advance techniques (IHC, molecular biology, immunofluorescence, ISH and so on) in the field of histotechnology. Having this credential is not going to hurt you, rather you will get more knowledge in the advance techniques in histology. Tunde Ajibade BS, HTL(ASCP)QIHC Histology Supervisor Medical Center Hospital Odessa,TX Tel:432-640-2348 Fax:432-640-2303 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Tuesday, November 15, 2011 11:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ?QIHC I WOULD LIKE TO KNOW ALSO Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From BDeBrosse-Serra <@t> isisph.com Tue Nov 15 12:32:54 2011 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Nov 15 12:33:09 2011 Subject: [Histonet] ?QIHC In-Reply-To: References: Message-ID: <493CAA64F203E14E8823737B9EE0E25F091FCE5910@EXCHMB01.isis.local> Great advice! I second that! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tunde Ajibade Sent: Tuesday, November 15, 2011 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ?QIHC Study for this certification gives you broad knowledge in all aspects of advance techniques (IHC, molecular biology, immunofluorescence, ISH and so on) in the field of histotechnology. Having this credential is not going to hurt you, rather you will get more knowledge in the advance techniques in histology. Tunde Ajibade BS, HTL(ASCP)QIHC Histology Supervisor Medical Center Hospital Odessa,TX Tel:432-640-2348 Fax:432-640-2303 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Tuesday, November 15, 2011 11:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ?QIHC I WOULD LIKE TO KNOW ALSO Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathologylab <@t> ymail.com Tue Nov 15 12:38:44 2011 From: pathologylab <@t> ymail.com (Pathology Lab) Date: Tue Nov 15 12:38:52 2011 Subject: [Histonet] Master Degree Investigation Project Message-ID: <1321382324.62584.YahooMailNeo@web121411.mail.ne1.yahoo.com> I am preparing?my thesis?for my?MBA?and my?dissertation?will be?on?the skills, knowledge?and attitudes that?should have?histotecnolgos?as?health professionals.Some of you?would like to help?me? Thanks ? Lcda. Mary V. Guerrero,BS, MBA,HtL ? ? ? ? ? ? ? Administradora/Coordinadora General ?Pathology Lab.? ? 55?N. Dr. Basora Edificio M?dico IV?Oficina 206 Mayaguez, Puerto Rico 00680 Tel. 787-834-8202 ?Fax: 787-831-5255 Sra. Dimary Valent?n?????????? Sra. Iris Franqui??????????????? Sra. Myrna Gonz?lez ? Facturaci?n???????? Reportes???????? ? ? Transcripcion This email may contain confidential health information that is legally privileged.? This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled.? If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited.? If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . From jkiernan <@t> uwo.ca Tue Nov 15 13:19:37 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Nov 15 13:19:45 2011 Subject: [Histonet] Lithium carbonate In-Reply-To: <7360c9d7974c2.4ec2bab5@uwo.ca> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B928C@PEITHA.wad.pa-ucl.com> <7360c9d7974c2.4ec2bab5@uwo.ca> Message-ID: <7490d05f97465.4ec274f9@uwo.ca> The solubility of Li2CO3 is 1.28% in cold water but only 0.71% in boiling water (data from the Merck Index). I've always kept on the lab shelf a bottle of saturated solution, with a little powder on the bottom. This keeps for ever and can be diluted 20-fold with water for such purposes as blueing haemalum or differentiating luxol fast blue. Lithium carbonate doesn't dissolve in alcohol, but that shouldn't be a problem. It's just a convenient, though rather expensive, source of not-too-alkaline water. John Kiernan Anatomy, UWO London, Canada = = = On 15/11/11, Nancy Schmitt wrote: > > Good Morning- > Making up some lithium carbonate - it is staying very milky in appearance - have not previously had that happen. We tried a couple different times - same result. We are not doing anything different - 5g lithium carbonate to 500ml Type I water. > Any thoughts? > Thanks, Nancy > > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rsrichmond <@t> gmail.com Tue Nov 15 13:23:05 2011 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Nov 15 13:23:16 2011 Subject: [Histonet] Re: counterstain for fast red Message-ID: In choosing a nuclear stain color I'd want to know if the pathologist has normal color vision - remember one man in eight doesn't. Green would not be a good counterstain for red IHC precipitate in a man with red-green color blindness. I'd ask him. The most serious problem I've seen with color-blind pathologists is that they can't read traditional light-microscopic acid-fast stains, with red bugs and blue background. There are work-arounds, but it's much better to do it by fluorescence (auramine O) or find a reference lab that can. Bob Richmond Samurai Pathologist (with normal color vision, though some who know my taste in clothing have disputed that) Knoxville TN On Tue, Nov 15, 2011 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. Slide and Cassette printing (Dana Spencer) > ? 2. Re: EM question (koellingr@comcast.net) > ? 3. Re:counterstain for fast red (Silvina Molinuevo) > ? 4. Processor list of cases for Competency (Jimmy Lofton) > ? 5. RE: Slide and Cassette printing (Janice Mahoney) > ? 6. ?QIHC (Sara Baldwin/mhhcc.org) > ? 7. RE: ?QIHC (Walter Benton) > ? 8. Second Shift lead position available at CellNetix Labs ? ?in > ? ? ?Seattle (Patrick Laurie) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 15 Nov 2011 09:17:06 -0500 > From: "Dana Spencer" > Subject: [Histonet] Slide and Cassette printing > To: histonet@lists.utsouthwestern.edu > Message-ID: <4EC22E11.536C.000A.0@PCMH.COM> > Content-Type: text/plain; charset=us-ascii > > What hardware are you using for printing slides and cassettes? ?There are many good systems out there and each have their flaws, but specifically I have heard negative rumblings about the PrintMate and SlideMate Systems from ThermoFisher. ?Are you using labels at the microtome? ?I would welcome any feedback or recommendations esp from larger institutions with large volumes. > > Thanks, > Dana Spencer, CT(ASCP) > Anatomic Pathology Manager > Pitt County Memorial Hospital > Greenville, NC ?27834 > > ------------------------------------------------------------------------------ > The contents of this e-mail (and any attachments) are confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. > ============================================================================== > > > ------------------------------ > > Message: 2 > Date: Tue, 15 Nov 2011 14:19:23 +0000 (UTC) > From: koellingr@comcast.net > Subject: Re: [Histonet] EM question > To: Cheryl Crowder > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ?<331082704.1898578.1321366763290.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> > > Content-Type: text/plain; charset=utf-8 > > > > > Cheryl, > > ? > > Have done??some things??with ordinary sectioning??cryo-electron microscopy but not your specific application.?? But can go to Cold Harbor Springs Protocols and they can get "Immersion Freezing of Cell Monolayers for Cryo-Electron Tomongraphy". > > ? > > Even better (more immediate) go here http://www.jove.com/video/1943/electron-cryotomography-of-bacterial-cells ?? and is a California Institute of Technology (really well produced) video/audio/written explaination of the whole procedure. > > ? > > Ray > > ? > > Ray Koelling > > PhenoPath Labs > > Seattle, WA > > > > ----- Original Message ----- > > > > > From: "Cheryl Crowder" > To: histonet@lists.utsouthwestern.edu > Sent: Monday, November 14, 2011 4:20:35 PM > Subject: [Histonet] EM question > > I am the first to admit I know little to nothing about EM. ??I have a > researcher who read an article today that said in the method that they had > taken cells, applied them to copper EM grids and immersed them in liquid > nitrogen to fix them. ??Has anyone ever heard of this technique? and if you > have, do you have any directions or references for this technique? > Cheryl > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 3 > Date: Tue, 15 Nov 2011 06:57:20 -0800 (PST) > From: Silvina Molinuevo > Subject: [Histonet] Re:counterstain for fast red > To: "histonet-bounces@lists.utsouthwestern.edu" > ? ? ? ?, > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<1321369040.38494.YahooMailNeo@web113603.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=utf-8 > > Hi Kim! light green ussually looks well with red background. > before you use it be sure to know how to prepare it! > best regards, sil > > ------------------------------ > > Message: 4 > Date: Tue, 15 Nov 2011 10:22:08 -0500 > From: "Jimmy Lofton" > Subject: [Histonet] Processor list of cases for Competency > To: Histonet@lists.utsouthwestern.edu > Message-ID: <4EC23D500200005600003F6A@nodcdmg2.no.trinity-health.org> > Content-Type: text/plain; charset=us-ascii > > Does anyone in histoland have a list of types of specimens that a histotechnologist who is processing specimens could be used for initial competency. > > Thanks, > > Jimmy > > > Jimmy Lofton, M.S., HT,CT(ASCP) > Manager Histology Laboratory > Holy Cross Hospital > 1500 Forest Glen Road > Silver Spring, MD ?20910-1484 > 301-754-7353?(Phone) > 301-754-8563?(Fax) > loftonjt@holycrosshealth.org > > > Trinity Health MailGate made the following annotations > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and privileged under state and Federal privacy laws. If you received this e-mail in error, be aware that any unauthorized use; disclosure, copying, or distribution is strictly prohibited. Please contact the sender immediately and destroy all copies of this message. > --------------------------------------------------------------------- > > > > ------------------------------ > > Message: 5 > Date: Tue, 15 Nov 2011 07:02:16 -1000 > From: Janice Mahoney > Subject: RE: [Histonet] Slide and Cassette printing > To: , histo net > ? ? ? ? > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Hi Dana, > We used the slide labels for the Vantage system without any problems for several years. ?We labeled at the cutting station and NEVER had a label wash off or smear in about three years. ?I highly recommend it. ?You will also see a huge gain in productivity elimination double labeling. > Hope all is well with you. ?Call me anytime, it would be great to hear form you. > Jan Mahoney > Omaha, NE > > >> Date: Tue, 15 Nov 2011 09:17:06 -0500 >> From: Dana.Spencer@PCMH.COM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Slide and Cassette printing >> >> What hardware are you using for printing slides and cassettes? There are many good systems out there and each have their flaws, but specifically I have heard negative rumblings about the PrintMate and SlideMate Systems from ThermoFisher. Are you using labels at the microtome? I would welcome any feedback or recommendations esp from larger institutions with large volumes. >> >> Thanks, >> Dana Spencer, CT(ASCP) >> Anatomic Pathology Manager >> Pitt County Memorial Hospital >> Greenville, NC 27834 >> >> ------------------------------------------------------------------------------ >> The contents of this e-mail (and any attachments) are confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. >> ============================================================================== >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 6 > Date: Tue, 15 Nov 2011 12:33:16 -0500 > From: "Sara Baldwin/mhhcc.org" > Subject: [Histonet] ?QIHC > To: "histonet@lists.utsouthwestern.edu " > ? ? ? ? > Message-ID: > ? ? ? ? > > Content-Type: text/plain; ? ? ? charset=ISO-8859-1 > > I WOULD LIKE TO KNOW ALSO > > Thanks > Histology/Cytology Supervisor > S. Kathy Baldwin, SCT (ASCP) > Memorial Hospital and Health Care Center > sbaldwin@mhhcc.org > Ph 812-996-0210, 0216, ?Fax 812-996-0232, > Pager 812-481-0897, Cell 812-887-3357 > Confidential information, Authorized use only. > > > ------------------------------ > > Message: 7 > Date: Tue, 15 Nov 2011 12:44:52 -0500 > From: Walter Benton > Subject: RE: [Histonet] ?QIHC > To: Sara Baldwin/mhhcc.org , > ? ? ? ?"histonet@lists.utsouthwestern.edu " > ? ? ? ? > Message-ID: > ? ? ? ?<0B8979A204680A42B93A52B486088CD9206C20444F@CUAEXH1.GCU-MD.local> > Content-Type: text/plain; charset="us-ascii" > > I think it is valuable since it allows you to learn things through study and application that you may not have been previously exposed to while preparing for the test. The test is strictly theory now versus the first year when stained slides were submitted and there was zero theory. Given the rapid changes in histology with ISH, IHC, IFs and other molecular based testing I don't see how it could hurt you to earn this credential. I'm not sure how pays is differentiated in the market for this qualification, but I do know that I see more and more jobs, especially those for IHC that require it or it is highly desirable for the position. > > Walter Benton HT(ASCP)QIHC > Histology Supervisor > Chesapeake Urology Associates > 806 Landmark Drive, Suite 126 > (All Deliveries to Suite 127) > Glen Burnie, MD 21061 > 443-471-5850?(Direct) > 410-768-5961?(Lab) > 410-768-5965?(Fax) > wbenton@cua.md > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org [sbaldwin@mhhcc.org] > Sent: Tuesday, November 15, 2011 12:33 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] ?QIHC > > I WOULD LIKE TO KNOW ALSO > > Thanks > Histology/Cytology Supervisor > S. Kathy Baldwin, SCT (ASCP) > Memorial Hospital and Health Care Center > sbaldwin@mhhcc.org > Ph 812-996-0210, 0216, ?Fax 812-996-0232, > Pager 812-481-0897, Cell 812-887-3357 > Confidential information, Authorized use only. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. ?If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. > > > > ------------------------------ > > Message: 8 > Date: Tue, 15 Nov 2011 09:51:01 -0800 > From: Patrick Laurie > Subject: [Histonet] Second Shift lead position available at CellNetix > ? ? ? ?Labs ? ?in Seattle > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=windows-1252 > > ?Histonet, > > > > > > *Job Title:* ? ? ? ? ? ? ? ? ? ?Lead Histotech ? 2nd shift > > *Department: ? * ? ? ? ? ? ?Laboratory > > *Reports To:* ? ? ? ? ? ? ? ?Histology Supervisor > > *Shifts: ? ? ? ? ? ? ? ? ? ? ? ? *3:00pm to 11:30PM Monday thru Friday > > *SUMMARY* > > Under the supervision of the Histology Supervisor, the Lead Histology > Technician/Technologist, performs with proficiency and understanding, the > tests and procedures assigned to the Histology Department. ?The position is > responsible for grossing, processing, embedding, cutting and staining of > tissue blocks, accurate reporting of results, quality control, teaching, > special projects, equipment maintenance and all other duties assigned by > the Supervisor. > > * * > > *ESSENTIAL FUNCTIONS* > > ? ? ? ? ?Performs both routine and complex special procedures with the > understanding of tissue structures, techniques, principles, theory and > instrumentation. > > ? ? ? ? ?Able to gross section and dictate descriptions of simple > specimens, process, embed, cut and stain all types of tissue specimens > received in the Histology Department. > > ? ? ? ? ?Coverslip stained sections, verify that the staining shows > adequate cellular distinction, label, and distribute to the pathologist > responsible for reviewing the case. > > ? ? ? ? ?Assures specimen labeling requirements are met and necessary > clinical information is available as needed. > > ? ? ? ? ?Assist pathologists with frozen section staining and processing. > > ? ? ? ? ?Assists laboratory assistant or performs accessioning of patient > specimens into laboratory computer system when needed. > > ? ? ? ? ?Demonstrates general knowledge of pathological and physiological > conditions that affect test results and tissue staining. > > ? ? ? ? ?Selects and maintains appropriate tissue blocks to serve as > controls for Special Satins. ?Maintains all documentation that insures that > all Special Stain control blocks have been adequately tested. ?Cuts and > labels control slides for Histology, Special Stains and IHC. > > ? ? ? ? ?Able to process and decal bone specimens. > > ? ? ? ? ?Recognize and troubleshoot both routine and complex problems and > assist other Histotechs and lab assistants with technical problems. > > ? ? ? ? ?Monitor quality control results and take immediate and proper > action when controls are unacceptable. ?Follow defined procedures with only > supervisor or pathologist approved modifications or deviations. > > ? ? ? ? ?Complete all instrument function verification, maintenance, and > documents according to procedure in Histology area. ?Ensure that equipment > defects and malfunctions are reported and repaired. > > ? ? ? ? ?Maintains a neat, clean, and orderly work area. ?At the end of the > day?s work; is responsible for the cleaning of his/her microtome, filing > his/ her cut blocks and cleaning any soiled glassware. > > ? ? ? ? ?Responsible for the proper handling and disposal of all > biohazardous materials and chemically hazardous materials including the > neutralization or recycling of chemicals before disposal or reuse. > > ? ? ? ? ?Cuts slides for IHC testing or other sendouts in conformance with > procedures and submits them for analysis. > > ? ? ? ? ?Takes on additional responsibility for training Laboratory > personnel including Laboratory Assistants, NRT?s and other Histotechs in > Histology procedures. > > ? ? ? ? ?Maybe be asked to write/ update Operating Procedures. > > ? ? ? ? ?Must be able to express himself/ herself effectively both in > written and verbal communications. > > ? ? ? ? ?Assists supervisory staff in monitoring workflow and insuring that > work/ staining priorities are met for the department. > > ? ? ? ? ?Will be asked to put new tests/ stains on line. > > ? ? ? ? ?May assist pathologists or physicians in the collection of > specimens from patients. > > ? ? ? ? ?Participates in special projects or other duties as assigned by > supervisor staff or company. > > ? ? ? ? ?Always maintains a pleasant, courteous attitude when answering the > telephone. > > ? ? ? ? ?Conforms to laboratory timekeeping procedures. ?Keeps unexcused > absences and late arrivals to a minimum. > > ? ? ? ? ?Participates in weekend and holiday schedules as staffing > requirements dictate. ?Remains flexible and works a share of overtime or > different shifts if necessary during staffing shortages or emergencies. > > ? ? ? ? ?Participates in continuing education classes and courses. ?Strongly > encouraged to keep updated on recent advances in the field of histology and > to take at least 10 credit hours of continuing education a year. > > ? ? ? ? ?Highly encouraged to maintain membership in a professional > Histology organization. > > ? ? ? ? ?Always maintains a safe work environment and attends all safety > training classes and conforms to all company safety guidelines and > requirements. > > ? ? ? ? ?Conforms to established and new procedures and policies instituted > by the company. > > ? ? ? ? ?Conforms to the Laboratory Dress code and wears appropriate name > badge at all times. > > ? ? ? ? ?Able to accept guidance and constructive criticism. ?Displays a > mature and professional attitude, is honest, and has a strong sense of > responsibility toward patients and clients. > > ? ? ? ? ?Able to do all the duties of a HT, Non ? Registered > Histotechnician, Lab Tech II, and Laboratory Assistant within the Histology > department. ?During heavy workloads or staffing shortages, the Histology > Technologist may be required to perform any or all of the duties in these > positions. > > We are seeking an experienced person for a lead position on 2nd shift with > hours from 3pm to 11:30pm. This is a hands-on position with the Lead > performing all histo duties in addition to being a trainer and answer > person for the shift. Five years histotech experience required with at > least 1 year in a Lead or higher role. Must be certified HT/HTL. > Please apply via the CellNetix website: > http://www.cellnetix.com/careers/job-openings > > Thanks, > -- > Patrick Laurie HT(ASCP)QIHC > CellNetix Pathology & Laboratories > 1124 Columbia Street, Suite 200 > Seattle, WA 98104 > plaurie@cellnetix.com > > > > -- > Patrick Laurie HT(ASCP)QIHC > CellNetix Pathology & Laboratories > 1124 Columbia Street, Suite 200 > Seattle, WA 98104 > plaurie@cellnetix.com > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 96, Issue 22 > **************************************** > From jkiernan <@t> uwo.ca Tue Nov 15 13:36:20 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Nov 15 13:36:27 2011 Subject: [Histonet] Master Degree Investigation Project In-Reply-To: <1321382324.62584.YahooMailNeo@web121411.mail.ne1.yahoo.com> References: <1321382324.62584.YahooMailNeo@web121411.mail.ne1.yahoo.com> Message-ID: <7510dde992306.4ec278e4@uwo.ca> Here's my helpful advice. Read a few books that cover the subject comprehensively. Two that I recommend are "Theory and Practice of Histological Techniques" by J. Bancroft and M. Gamble (ISBN 9780443102790), and "Histopathology: A Self-Instructional Text" by Freida L. Carson. You can find information about the career of histotechnologist, especially in the USA, on the web site of the National Society for Histotechnology. Armed with this information you should be able to start writing your dissertation. John Kiernan Anatomy, UWO London, Canada = = = On 15/11/11, Pathology Lab wrote: > > I am preparing my thesis for my MBA and my dissertation will be on the skills, knowledge and attitudes that should have histotecnolgos as health professionals.Some of you would like to help me? > > > Thanks > > Lcda. Mary V. Guerrero,BS, MBA,HtL > Administradora/Coordinadora General > Pathology Lab. > 55 N. Dr. Basora Edificio M?dico IV Oficina 206 > Mayaguez, Puerto Rico 00680 > Tel. 787-834-8202 Fax: 787-831-5255 > > > > Sra. Dimary Valent?n Sra. Iris Franqui Sra. Myrna Gonz?lez > Facturaci?n Reportes Transcripcion > > > From arlange <@t> medicine.nevada.edu Tue Nov 15 13:43:15 2011 From: arlange <@t> medicine.nevada.edu (Alicia R. Lange) Date: Tue Nov 15 13:43:24 2011 Subject: [Histonet] Paraffin Message-ID: <9809822F80B1414C90EB4438713C8968F265A0@MEDX.medicine.nevada.edu> Hello All, Has anyone tried the Tek-select paraffin from International Medical equipment? Their flyer advertises great comments from histotechs but I would like real verification. J Alicia Lange Laboratory Supervisor University of Nevada, Reno Pathology Department arlange@medicine.nevada.edu From ddubs0001 <@t> msn.com Tue Nov 15 14:15:58 2011 From: ddubs0001 <@t> msn.com (=?utf-8?B?ZGR1YnMwMDAxQG1zbi5jb20=?=) Date: Tue Nov 15 14:15:53 2011 Subject: [Histonet] Re: Histonet Digest, Vol 96, Issue 22 Message-ID: Sent from my Verizon Wireless Phone ----- Reply message ----- From: histonet-request@lists.utsouthwestern.edu Date: Tue, Nov 15, 2011 12:00 pm Subject: Histonet Digest, Vol 96, Issue 22 To: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Slide and Cassette printing (Dana Spencer) 2. Re: EM question (koellingr@comcast.net) 3. Re:counterstain for fast red (Silvina Molinuevo) 4. Processor list of cases for Competency (Jimmy Lofton) 5. RE: Slide and Cassette printing (Janice Mahoney) 6. ?QIHC (Sara Baldwin/mhhcc.org) 7. RE: ?QIHC (Walter Benton) 8. Second Shift lead position available at CellNetix Labs in Seattle (Patrick Laurie) ---------------------------------------------------------------------- Message: 1 Date: Tue, 15 Nov 2011 09:17:06 -0500 From: "Dana Spencer" Subject: [Histonet] Slide and Cassette printing To: histonet@lists.utsouthwestern.edu Message-ID: <4EC22E11.536C.000A.0@PCMH.COM> Content-Type: text/plain; charset=us-ascii What hardware are you using for printing slides and cassettes? There are many good systems out there and each have their flaws, but specifically I have heard negative rumblings about the PrintMate and SlideMate Systems from ThermoFisher. Are you using labels at the microtome? I would welcome any feedback or recommendations esp from larger institutions with large volumes. Thanks, Dana Spencer, CT(ASCP) Anatomic Pathology Manager Pitt County Memorial Hospital Greenville, NC 27834 ------------------------------------------------------------------------------ The contents of this e-mail (and any attachments) are confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. ============================================================================== ------------------------------ Message: 2 Date: Tue, 15 Nov 2011 14:19:23 +0000 (UTC) From: koellingr@comcast.net Subject: Re: [Histonet] EM question To: Cheryl Crowder Cc: histonet@lists.utsouthwestern.edu Message-ID: <331082704.1898578.1321366763290.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Content-Type: text/plain; charset=utf-8 From pruegg <@t> ihctech.net Tue Nov 15 14:26:28 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Nov 15 14:26:38 2011 Subject: [Histonet] is there a way to recover ruined tissues Message-ID: <1C9222417F0C4A288D9B90B151AB24A0@prueggihctechlt> My tissue processor was loaded and although the technician thought they started it, the next morning the tissues were found dry in the retort with the processor not started. Another technician just started the machine so the tissues got processed as they would have been had it started the day before. The tissues look terrible, they shrank so much that the pieces started coming out of the cassettes even though they were plenty large enough in the first place. The tissues were found to be very small and hard but were embedded, sectioned and stained anyway (I was not informed about this until I started questioning why the tissues looked so bad). Of course the investigator is very upset that their tissues are ruined. Does anyone know if there is any way to try and recovery these tissues? They were mouse lungs and livers. The livers are not as bad as the lungs, the lungs were inflated and they look compressed and unrecognizable as lung tissue. If the investigator will ever speak to me again I am going to ask for the blocks back and try melting them down and perhaps try to rehydrate them somehow for reprocessing. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org From DKnutson <@t> primecare.org Tue Nov 15 14:31:54 2011 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Tue Nov 15 14:32:04 2011 Subject: [Histonet] 2012 CPT Codes Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2C49C8F58@EXCHANGE2K7.staprimecare.org> Hello Fellow Histonet Followers, I was wondering if anyone has had a chance to look through the 2012 pathology CPT codes yet? There is a revision on 88312, 88313, 88314, and 88319. The revision states to report one unit of the code for each special stain, on each surgical pathology block, cytologic specimen, or hematologic smear. As an example, let's say we have a specimen where blocks A1, A2, A3, and A4 were grossed in. The pathologist orders a GMS stain on both A1 and A2. Right now we would charge an 88312 X 1. But is the correct interpretation of the 2012 revision mean that in 2012 with this same scenario, we would charge an 88312 X 2? Or does block mean specimen and we keep billing as we do now? If block means specimen, then the CPT codes concerning frozen blocks (88331 and 88332) don't make sense. I am confused and would appreciate how the rest of you are interpreting these revisions. Another question I have is code 88314. Does this code only to be used during Mohs surgery? Or can it be used during a regular pathology frozen section procedure where a special stain is ordered on a frozen tissue block? Again, thank you for your assistance and thoughts. Deanne Knutson dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From Carol.Fields <@t> Northside.com Tue Nov 15 14:50:54 2011 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Tue Nov 15 14:51:08 2011 Subject: [Histonet] Job Opening Message-ID: <731941C266951A47BEF11E5EFAAED9C90B666D91@nsmvexch01.northside.local> Northside Hospital Atlanta, GA has an opening for a FT Histo Tech. Please apply on line at http://www.northside.com/careers/main.aspx Send resume to dana.bible @northside.com and details on the position. Thank you, Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From rjbuesa <@t> yahoo.com Tue Nov 15 15:00:47 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 15 15:00:55 2011 Subject: [Histonet] ?QIHC In-Reply-To: Message-ID: <1321390847.69248.YahooMailClassic@web65715.mail.ac4.yahoo.com> Knowledge never hurts. Just being forced to pass a test will also force you to understand the procedures and will make you more able to trouble shoot your own work. On the other hand, even if having this certification does not increase your pay scale, it may be a "plus" in the event you decide to change jobs and apply for a new position. That "extra certificate" may be what you need to land a better job. Go for it! Ren? J. --- On Tue, 11/15/11, Sara Baldwin/mhhcc.org wrote: From: Sara Baldwin/mhhcc.org Subject: [Histonet] ?QIHC To: "histonet@lists.utsouthwestern.edu " Date: Tuesday, November 15, 2011, 12:33 PM I WOULD LIKE TO KNOW ALSO Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216,? Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Nov 15 15:10:27 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 15 15:10:36 2011 Subject: [Histonet] Master Degree Investigation Project In-Reply-To: <1321382324.62584.YahooMailNeo@web121411.mail.ne1.yahoo.com> Message-ID: <1321391427.43865.YahooMailClassic@web65701.mail.ac4.yahoo.com> I feel somewhat uncomfortable by your question because?it seems that you are not a histotech but on the other hand are trying to being able to tall what a histotech should do and this is quite a "peculiar" situation. This would not be?a problem had you been a histotech?now projecting to sum up your personal?experiences in a critical and?useful way for others to follow, but it seems that is not the case. I strongly advise to try to visit several histology labs, get involved in the process, talk with histotechs,?supervisors, managers and?pathologists (yes, they also have a saying in this issue)?to try to have at least an idea of what you intend to write a master thesis about. After you do that perhaps you will be more able to have an idea of what you are going to write about. Ren? J.? --- On Tue, 11/15/11, Pathology Lab wrote: From: Pathology Lab Subject: [Histonet] Master Degree Investigation Project To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, November 15, 2011, 1:38 PM I am preparing?my thesis?for my?MBA?and my?dissertation?will be?on?the skills, knowledge?and attitudes that?should have?histotecnolgos?as?health professionals.Some of you?would like to help?me? Thanks ? Lcda. Mary V. Guerrero,BS, MBA,HtL ? ? ? ? ? ? ? Administradora/Coordinadora General ?Pathology Lab.? ? 55?N. Dr. Basora Edificio M?dico IV?Oficina 206 Mayaguez, Puerto Rico 00680 Tel. 787-834-8202 ?Fax: 787-831-5255 Sra. Dimary Valent?n?????????? Sra. Iris Franqui??????????????? Sra. Myrna Gonz?lez ? Facturaci?n???????? Reportes???????? ? ? Transcripcion This email may contain confidential health information that is legally privileged.? This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled.? If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited.? If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Nov 15 15:12:54 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 15 15:13:03 2011 Subject: [Histonet] Paraffin In-Reply-To: <9809822F80B1414C90EB4438713C8968F265A0@MEDX.medicine.nevada.edu> Message-ID: <1321391574.88501.YahooMailClassic@web65702.mail.ac4.yahoo.com> And are you surprised that the seller of the product includes glowing comments from "users"? If you are satisfied with the paraffin you are using now, why would you like to change? Change is not always better! Ren? J. --- On Tue, 11/15/11, Alicia R. Lange wrote: From: Alicia R. Lange Subject: [Histonet] Paraffin To: histonet@lists.utsouthwestern.edu Date: Tuesday, November 15, 2011, 2:43 PM Hello All, Has anyone tried the Tek-select paraffin from International Medical equipment? Their flyer advertises great comments from histotechs but I would like real verification. J Alicia Lange Laboratory Supervisor University of Nevada, Reno Pathology Department arlange@medicine.nevada.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Nov 15 15:14:20 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 15 15:14:29 2011 Subject: [Histonet] is there a way to recover ruined tissues In-Reply-To: <1C9222417F0C4A288D9B90B151AB24A0@prueggihctechlt> Message-ID: <1321391660.37697.YahooMailClassic@web65714.mail.ac4.yahoo.com> Realy recovered? No! Ren? J. --- On Tue, 11/15/11, Patsy Ruegg wrote: From: Patsy Ruegg Subject: [Histonet] is there a way to recover ruined tissues To: "'histo net'" Date: Tuesday, November 15, 2011, 3:26 PM My tissue processor was loaded and although the technician thought they started it, the next morning the tissues were found dry in the retort with the processor not started.? Another technician just started the machine so the tissues got processed as they would have been had it started the day before.? The tissues look terrible, they shrank so much that the pieces started coming out of the cassettes even though they were plenty large enough in the first place.? The tissues were found to be very small and hard but were embedded, sectioned and stained anyway (I was not informed about this until I started questioning why the tissues looked so bad). Of course the investigator is very upset that their tissues are ruined. Does anyone know if there is any way to try and recovery these tissues? They were mouse lungs and livers.? The livers are not as bad as the lungs, the lungs were inflated and they look compressed and unrecognizable as lung tissue.? If the investigator will ever speak to me again I am going to ask for the blocks back and try melting them down and perhaps try to rehydrate them somehow for reprocessing. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mvgeducationaresources <@t> gmail.com Tue Nov 15 15:37:01 2011 From: mvgeducationaresources <@t> gmail.com (Mary Guerrero) Date: Tue Nov 15 15:37:12 2011 Subject: [Histonet] Master Degree Investigation Project In-Reply-To: <7510dde992306.4ec278e4@uwo.ca> References: <1321382324.62584.YahooMailNeo@web121411.mail.ne1.yahoo.com> <7510dde992306.4ec278e4@uwo.ca> Message-ID: <0632FBF4-B2E6-46C8-931F-7F86B6BE68C5@gmail.com> Thanks John for answer me Sent from my iPhone On Nov 15, 2011, at 3:36 PM, John Kiernan wrote: > Here's my helpful advice. Read a few books that cover the subject comprehensively. Two that I recommend are "Theory and Practice of Histological Techniques" by J. Bancroft and M. Gamble (ISBN 9780443102790), and > "Histopathology: A Self-Instructional Text" by Freida L. Carson. You can find information about the career of histotechnologist, especially in the USA, on the web site of the National Society for Histotechnology. > Armed with this information you should be able to start writing your dissertation. > > John Kiernan > Anatomy, UWO > London, Canada > = = = > On 15/11/11, Pathology Lab wrote: > >> >> I am preparing my thesis for my MBA and my dissertation will be on the skills, knowledge and attitudes that should have histotecnolgos as health professionals.Some of you would like to help me? >> >> >> Thanks >> >> Lcda. Mary V. Guerrero,BS, MBA,HtL >> Administradora/Coordinadora General >> Pathology Lab. >> 55 N. Dr. Basora Edificio M?dico IV Oficina 206 >> Mayaguez, Puerto Rico 00680 >> Tel. 787-834-8202 Fax: 787-831-5255 >> >> >> >> Sra. Dimary Valent?n Sra. Iris Franqui Sra. Myrna Gonz?lez >> Facturaci?n Reportes Transcripcion >> >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lydia.gunawan <@t> unimelb.edu.au Tue Nov 15 15:47:01 2011 From: lydia.gunawan <@t> unimelb.edu.au (Lydia Gunawan) Date: Tue Nov 15 15:47:18 2011 Subject: [Histonet] help Message-ID: <5C988DCFFE80014695B544D31BA8AA8A40FC9D58@000s-ex-mbx-qs1.unimelb.edu.au> Lydia Gunawan Oxidation Biology Laboratory Mental Health Research Institute Melbourne Brain Centre Corner Royal Pde and Genetics Lane University of Melbourne, Level 4 Parkville, Vic 3010 email: lydia.gunawan@unimelb.edu.au From lydia.gunawan <@t> unimelb.edu.au Tue Nov 15 16:00:32 2011 From: lydia.gunawan <@t> unimelb.edu.au (Lydia Gunawan) Date: Tue Nov 15 16:01:50 2011 Subject: [Histonet] help Message-ID: <5C988DCFFE80014695B544D31BA8AA8A40FC9D73@000s-ex-mbx-qs1.unimelb.edu.au> Hi there, I am having trouble with Turnbull staining. Anybody can help me? As I want to detect and quantify iron in the brain for Parkinson experiment, I am using mice(C57Bl6) brain that were treated with MPTP injection. So, I have been trying to stain those brain using paraffin section with Turnbull blue but I have no luck. FYI, I have been using 7% of Potassium ferricyanide in 3% HCL and I incubated for 2 hours, 37'. I also did incubation in triton-x and H2O2 too but still not getting any iron on my sections. Could anyone help me to solve my problem? Thanks Lydia Gunawan Oxidation Biology Laboratory Mental Health Research Institute Melbourne Brain Centre Corner Royal Pde and Genetics Lane University of Melbourne, Level 4 Parkville, Vic 3010 email: lydia.gunawan@unimelb.edu.au From CIngles <@t> uwhealth.org Tue Nov 15 16:00:22 2011 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Nov 15 16:02:55 2011 Subject: [Histonet] RE: 15 years of Histonet References: <8D7C2D242DBD45498006B21122072BF89F5EE478@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A787@UWHC-MAIL2.uwhis.hosp.wisc.edu> I didn't think space had a "side" anyway.?. You're not an egomaniac, You are just really proud of the work you do! Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Mon 11/14/2011 4:36 PM To: Morken, Timothy; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: 15 years of Histonet Can't agree more. I mean, where would Joe the Toe be without the Histonet? By the way, just read the reviews from the lecture my buddy Hector and I gave in Cinci this year. One of the critiques stated that Hector and I are the biggest egomaniacs this side of space. Wow, I've been called a lot of things in my life, but an egomaniac? Really? That hurt, right to the core. From tony.henwood <@t> health.nsw.gov.au Tue Nov 15 19:01:28 2011 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Nov 15 19:01:42 2011 Subject: [Histonet] RE: help In-Reply-To: <5C988DCFFE80014695B544D31BA8AA8A40FC9D73@000s-ex-mbx-qs1.unimelb.edu.au> References: <5C988DCFFE80014695B544D31BA8AA8A40FC9D73@000s-ex-mbx-qs1.unimelb.edu.au> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A05C18@xmdb02.nch.kids> If you are trying to detect small amounts of iron, then possibly post DAB (that's right same as used for IPX) might be useful. Meguro R, Asano Y, Adagiri S, Li C, Iwatsuki H, Shoumura K (2007) "Nonheme-iron histochemistry for light and electron microscopy: a historical, theoretical and technical review" Arch Histol Cytol 70(1): 1-19. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lydia Gunawan Sent: Wednesday, 16 November 2011 9:01 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] help Hi there, I am having trouble with Turnbull staining. Anybody can help me? As I want to detect and quantify iron in the brain for Parkinson experiment, I am using mice(C57Bl6) brain that were treated with MPTP injection. So, I have been trying to stain those brain using paraffin section with Turnbull blue but I have no luck. FYI, I have been using 7% of Potassium ferricyanide in 3% HCL and I incubated for 2 hours, 37'. I also did incubation in triton-x and H2O2 too but still not getting any iron on my sections. Could anyone help me to solve my problem? Thanks Lydia Gunawan Oxidation Biology Laboratory Mental Health Research Institute Melbourne Brain Centre Corner Royal Pde and Genetics Lane University of Melbourne, Level 4 Parkville, Vic 3010 email: lydia.gunawan@unimelb.edu.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From gaviotalopez_pr <@t> hotmail.com Tue Nov 15 19:07:23 2011 From: gaviotalopez_pr <@t> hotmail.com (Zoe rosa) Date: Tue Nov 15 19:07:26 2011 Subject: [Histonet] CMV validation Message-ID: Hello there, I need to validate CMV in IHC, but I'm having trouble getting some to tissue to test. Can someone help with this? Thanks Zoe From jkiernan <@t> uwo.ca Tue Nov 15 22:54:50 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Nov 15 22:54:59 2011 Subject: [Histonet] Paraffin - Why change In-Reply-To: <1321391574.88501.YahooMailClassic@web65702.mail.ac4.yahoo.com> References: <9809822F80B1414C90EB4438713C8968F265A0@MEDX.medicine.nevada.edu> <1321391574.88501.YahooMailClassic@web65702.mail.ac4.yahoo.com> Message-ID: <7690dd2d9c19b.4ec2fbca@uwo.ca> As usual, Rene Buesa has a good sense of what might be worth changing - Do not change anything to please a salesman. "Change is inevitable. In a progressive country change is constant." (Benjamin Disraeli, 1804-1887, cited from the Oxford Dictionary of Quotations). Dizzie was Britain's first Conservative (as distinct from Tory or Whig) prime minister. He also had quite long hair (his own), was a Christian Jew, wrote popular light romance novels, and wore clothes that were funny even in his own time; a colourful character. As a well educated conservative, Dizzie tried to change things only for good reasons. Had he worked in a path lab, he probably would have adopted paraffin embedding and formaldehyde (had he lived long enough). Enough fun for now, John Kiernan = = = On 15/11/11, Rene J Buesa wrote: > > And are you surprised that the seller of the product includes glowing comments from "users"? > If you are satisfied with the paraffin you are using now, why would you like to change? > Change is not always better! > Ren? J. > > --- On Tue, 11/15/11, Alicia R. Lange wrote: > > > From: Alicia R. Lange > Subject: [Histonet] Paraffin > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, November 15, 2011, 2:43 PM > > > Hello All, > > Has anyone tried the Tek-select paraffin from International Medical > equipment? Their flyer advertises great comments from histotechs but I > would like real verification. J > > > > Alicia Lange > > > > Laboratory Supervisor > > University of Nevada, Reno > > Pathology Department > > arlange@medicine.nevada.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From lpwenk <@t> sbcglobal.net Wed Nov 16 04:25:47 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Nov 16 04:25:48 2011 Subject: [Histonet] Lithium carbonate In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B928C@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B928C@PEITHA.wad.pa-ucl.com> Message-ID: Any chance it should have been 0.5 g lithium carbonate in 500 mL water? Maybe someone typed the label wrong? That's happened to us before. Is it supposed to be a 1.0% solution, or a 0.1% solution? Only reason I'm asking is that the only place we use lithium carbonate is in the LFB stain for myelin, and we use a 0.05% solution (0.25 g in 500 mL). If you are adding 5 g to 500 mL of water, that's a 1% solution, which, for lithium carbonate, is very close to being a saturated solution (where not all of it will dissolve). Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 (The opinions expressed are mine, and do not reflect on my hospital.) -----Original Message----- From: Nancy Schmitt Sent: Tuesday, November 15, 2011 8:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lithium carbonate Good Morning- Making up some lithium carbonate - it is staying very milky in appearance - have not previously had that happen. We tried a couple different times - same result. We are not doing anything different - 5g lithium carbonate to 500ml Type I water. Any thoughts? Thanks, Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Wed Nov 16 06:59:19 2011 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Nov 16 06:59:33 2011 Subject: [Histonet] help In-Reply-To: <5C988DCFFE80014695B544D31BA8AA8A40FC9D73@000s-ex-mbx-qs1.unimelb.edu.au> Message-ID: <1300126180.1942476.1321448359281.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Hi Lydia, I think Tony Henwood has it exactly right in talking of DAB intensification.? The article he sites and several others show how much more sensitive DAB intensification can make an ordinary iron reaction.? And you are not looking in bone marrow or spleen but somewhere where there is so little iron. ? More than once I've been burned by?research colleagues?who gave me tissue saying "x" should be?upregulated and spend weeks looking for it until they say "oop's, sorry-my bad". Intra-dermal injections can become sub-dermal. IP injections can be sub-optimal with rough handling of mice. IV tail vein injections can be missed. And MPTP is toxic and dangerous enough to work with that I'd be fumbling around and nervous myself.? If you are confident the model is working by some secondary marker such as tyrosine hydroxylase immunoreactivity then you are still are looking for minute quantities of iron. ? Several easily available papers on that very mouse model tell you the limited cell population to look for (one says-NOT in the big cells), and in a very specific region using coronal sections (I always used a mouse brain mold to section to be sure of the anatomical location) and several of the papers?use classical iron histochemistry followed by DAB intensification and their procedures?are in material and methods. ? Reaction might be working after all-just have to focus in on very limited reaction product.? Good luck hunting. ? Ray ? Ray Koelling PhenoPath Labs Seattle, WA ----- Original Message ----- From: "Lydia Gunawan" To: Histonet@lists.utsouthwestern.edu Sent: Tuesday, November 15, 2011 2:00:32 PM Subject: [Histonet] help Hi there, I am having trouble with Turnbull staining. Anybody can help me? As I want to detect and quantify iron in the brain for Parkinson experiment, I am using mice(C57Bl6) ?brain that were treated with MPTP injection. ?So, I have been trying to stain those brain using paraffin section with Turnbull blue but I have no luck. FYI, I have been using 7% of Potassium ferricyanide in 3% HCL and I incubated for 2 hours, 37'. I also did incubation in triton-x and H2O2 too but still not getting any iron on my sections. Could anyone help me to solve my problem? Thanks Lydia Gunawan Oxidation Biology Laboratory Mental Health Research Institute Melbourne Brain Centre Corner Royal Pde and Genetics Lane University of Melbourne, Level 4 Parkville, Vic 3010 email: lydia.gunawan@unimelb.edu.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christen <@t> vet.k-state.edu Wed Nov 16 09:05:38 2011 From: christen <@t> vet.k-state.edu (Shelly Christenson) Date: Wed Nov 16 09:05:48 2011 Subject: [Histonet] is there a way to recover ruined tissues In-Reply-To: <1C9222417F0C4A288D9B90B151AB24A0@prueggihctechlt> References: <1C9222417F0C4A288D9B90B151AB24A0@prueggihctechlt> Message-ID: <06E342B6098ED9478347E1407764C80404B13411@VETMXHT.ads.vet.k-state.edu> Patsy, I have used this reprocessing schedule from Lee Luna's book "Histopathologic Methods and Color Atlas of Specials Stains and Tissue Artifact". This procedure has worked for me on a couple of occasions, You may need to change the times a little since your tissue samples are so small. Luna's book has a lot of information that is very helpful, and would be a good reference book to have in the lab. Hope this helps you out, give it a try. Tissue Softening Solution The following solution is used for softening dried tissue specimens prior to reprocessing or processing Formol-Sodium Acetate (Stock) Solution Concentrated formalin (37%) solution 10 ml Sodium acetate 2 gm Tap water 90 ml Formol-Glycerol (Working) Solution Formalin-sodium acetate (stock) 90 ml Glycerol (glycerin) 10 ml Reprocessing Schedule Melt the paraffin blocks down Place the tissue in 3 changes of xylene for 1 hour each Place in 100% alcohol, 2 changes for 1 hour each Place in 95% alcohol, 2 changes for 1 hour each Place under running tap water for 20 minutes Place in formol-glycerol working solution until tissue becomes soft (In most instances this will occur within 5-8 hours: extended exposure to the formol-gycerol will not harm tissue specimens) Place the tissue in the process and proceed with routine Processing schedule. How ever it is not necessary for the tissue to go through the fixative stations on the processor Best of Luck, Shelly Shelly Christenson HT (ASCP) Veterinary Diagnostic Laboratory Histopathology L-216 Mosier Hall Kansas State University 785/532-4464 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Patsy Ruegg [pruegg@ihctech.net] Sent: Tuesday, November 15, 2011 2:26 PM To: 'histo net' Subject: [Histonet] is there a way to recover ruined tissues My tissue processor was loaded and although the technician thought they started it, the next morning the tissues were found dry in the retort with the processor not started. Another technician just started the machine so the tissues got processed as they would have been had it started the day before. The tissues look terrible, they shrank so much that the pieces started coming out of the cassettes even though they were plenty large enough in the first place. The tissues were found to be very small and hard but were embedded, sectioned and stained anyway (I was not informed about this until I started questioning why the tissues looked so bad). Of course the investigator is very upset that their tissues are ruined. Does anyone know if there is any way to try and recovery these tissues? They were mouse lungs and livers. The livers are not as bad as the lungs, the lungs were inflated and they look compressed and unrecognizable as lung tissue. If the investigator will ever speak to me again I am going to ask for the blocks back and try melting them down and perhaps try to rehydrate them somehow for reprocessing. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Wed Nov 16 10:09:54 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Nov 16 10:10:07 2011 Subject: [Histonet] is there a way to recover ruined tissues In-Reply-To: <06E342B6098ED9478347E1407764C80404B13411@VETMXHT.ads.vet.k-state.edu> References: <1C9222417F0C4A288D9B90B151AB24A0@prueggihctechlt> <06E342B6098ED9478347E1407764C80404B13411@VETMXHT.ads.vet.k-state.edu> Message-ID: Patsy I have used a technique similar to the one in this article when I was fortunate to work on some mummified tissue, with surprising success, when I was back in Glasgow many, many moons ago Mekota A-M, Vermehren M Determination of optimal rehydration, fixation and staining methods for histological and immunohistochemical analysis of mummified soft tissues. Biotechnic & Histochemistry 2005, 80(1): 7-13 Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shelly Christenson Sent: Wednesday, November 16, 2011 10:06 AM To: Patsy Ruegg; 'histo net' Subject: RE: [Histonet] is there a way to recover ruined tissues Patsy, I have used this reprocessing schedule from Lee Luna's book "Histopathologic Methods and Color Atlas of Specials Stains and Tissue Artifact". This procedure has worked for me on a couple of occasions, You may need to change the times a little since your tissue samples are so small. Luna's book has a lot of information that is very helpful, and would be a good reference book to have in the lab. Hope this helps you out, give it a try. Tissue Softening Solution The following solution is used for softening dried tissue specimens prior to reprocessing or processing Formol-Sodium Acetate (Stock) Solution Concentrated formalin (37%) solution 10 ml Sodium acetate 2 gm Tap water 90 ml Formol-Glycerol (Working) Solution Formalin-sodium acetate (stock) 90 ml Glycerol (glycerin) 10 ml Reprocessing Schedule Melt the paraffin blocks down Place the tissue in 3 changes of xylene for 1 hour each Place in 100% alcohol, 2 changes for 1 hour each Place in 95% alcohol, 2 changes for 1 hour each Place under running tap water for 20 minutes Place in formol-glycerol working solution until tissue becomes soft (In most instances this will occur within 5-8 hours: extended exposure to the formol-gycerol will not harm tissue specimens) Place the tissue in the process and proceed with routine Processing schedule. How ever it is not necessary for the tissue to go through the fixative stations on the processor Best of Luck, Shelly Shelly Christenson HT (ASCP) Veterinary Diagnostic Laboratory Histopathology L-216 Mosier Hall Kansas State University 785/532-4464 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Patsy Ruegg [pruegg@ihctech.net] Sent: Tuesday, November 15, 2011 2:26 PM To: 'histo net' Subject: [Histonet] is there a way to recover ruined tissues My tissue processor was loaded and although the technician thought they started it, the next morning the tissues were found dry in the retort with the processor not started. Another technician just started the machine so the tissues got processed as they would have been had it started the day before. The tissues look terrible, they shrank so much that the pieces started coming out of the cassettes even though they were plenty large enough in the first place. The tissues were found to be very small and hard but were embedded, sectioned and stained anyway (I was not informed about this until I started questioning why the tissues looked so bad). Of course the investigator is very upset that their tissues are ruined. Does anyone know if there is any way to try and recovery these tissues? They were mouse lungs and livers. The livers are not as bad as the lungs, the lungs were inflated and they look compressed and unrecognizable as lung tissue. If the investigator will ever speak to me again I am going to ask for the blocks back and try melting them down and perhaps try to rehydrate them somehow for reprocessing. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From mpowers <@t> dpspa.com Wed Nov 16 11:51:56 2011 From: mpowers <@t> dpspa.com (Marian Powers) Date: Wed Nov 16 11:52:02 2011 Subject: [Histonet] IHC control Message-ID: Hi: Would anyone have a hereditary nonpolyposis colorectal cancer control, (for MHL1, MSH2, MSH6) to share? Thanks in advance, -- Marian L. Powers *Doctors Pathology Services * ** c| 302.747.0580 o| 302.677.0000 ext: 110 f | 302.677.0010 From amber.mckenzie <@t> gastrodocs.net Wed Nov 16 12:20:31 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Nov 16 12:19:30 2011 Subject: [Histonet] Validation In-Reply-To: References: Message-ID: <5A33C952BB67F4468AF1F36D739212BC06303C@JERRY.Gia.com> Just wondering what y'alls opinion is on validation: I don't really understand why optimization isn't enough. At that point, the pathologist has said what he wanted the stain to look like, so why do 3-10 positive slides on the new instrument to compare to previous slides from another instrument? Thanks! From joelleweaver <@t> hotmail.com Wed Nov 16 12:25:40 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Nov 16 12:25:44 2011 Subject: [Histonet] Validation In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC06303C@JERRY.Gia.com> References: , <5A33C952BB67F4468AF1F36D739212BC06303C@JERRY.Gia.com> Message-ID: Here is a link to a CAP presentation from their website, maybe this will help out. http://www.cap.org/apps/docs/education/lapaudio/pdf/051910_pres.pdf Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > From: amber.mckenzie@gastrodocs.net > To: histonet@lists.utsouthwestern.edu > Date: Wed, 16 Nov 2011 18:20:31 +0000 > Subject: [Histonet] Validation > > > Just wondering what y'alls opinion is on validation: I don't really understand why optimization isn't enough. At that point, the pathologist has said what he wanted the stain to look like, so why do 3-10 positive slides on the new instrument to compare to previous slides from another instrument? Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Wed Nov 16 12:27:08 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Nov 16 12:30:38 2011 Subject: [Histonet] RE: Validation In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC06303C@JERRY.Gia.com> References: , <5A33C952BB67F4468AF1F36D739212BC06303C@JERRY.Gia.com> Message-ID: <0B8979A204680A42B93A52B486088CD9206C20446B@CUAEXH1.GCU-MD.local> Optimizing is getting the stain to look a certain way based on retrieval, incubations times etc.... validation is the process of running cases that are known positives of varying levels(degrees of positivity) and running them with normal cases to ensure the antibody is staining as intended based upon the optimized protocol that you have established. If for some reason the cases display background or do not stain the positive and negative cases as predicted based upon your optimization the antibody should not be validated as being ready for clinical use, it should be re-worked. Hope this helps. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie [amber.mckenzie@gastrodocs.net] Sent: Wednesday, November 16, 2011 1:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Validation Just wondering what y'alls opinion is on validation: I don't really understand why optimization isn't enough. At that point, the pathologist has said what he wanted the stain to look like, so why do 3-10 positive slides on the new instrument to compare to previous slides from another instrument? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From robinsoc <@t> mercyhealth.com Wed Nov 16 12:31:10 2011 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Wed Nov 16 12:31:18 2011 Subject: [Histonet] Validation In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC06303C@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BC06303C@JERRY.Gia.com> Message-ID: <4EC3AD0E020000AF00006821@nodcdmg2.no.trinity-health.org> Amber, Optimizing the antibody is the first step of validation. Running the new protocol against previously stained cases is the second step and shows you are getting results as good as, if not better, with the new vs the old. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com >>> Amber McKenzie 11/16/2011 12:20 PM >>> Just wondering what y'alls opinion is on validation: I don't really understand why optimization isn't enough. At that point, the pathologist has said what he wanted the stain to look like, so why do 3-10 positive slides on the new instrument to compare to previous slides from another instrument? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Nov 16 12:41:49 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Nov 16 12:41:54 2011 Subject: [Histonet] RE: Validation - a little detail In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC06303C@JERRY.Gia.com> Message-ID: <14E2C6176416974295479C64A11CB9AE011380AC70C4@SBS2K8.premierlab.local> Amber When you optimize a stain you are normally just looking at one tissue type or possible a couple, not sure how you handle protocol development. The amount of work that is required for validation in the clinical setting is dependent upon the type of primary antibody that you are using. If it is an IVD antibody I believe that verification is only required. You need to verify that this antibody works as stated in your laboratory. If you are using an RUO antibody you have go through a validation process. This in my opinion includes two steps. 1. protocol development and optimization 2. validation - you are checking for sensitivity, specificity and reproducibility on multiple tissue samples The CAP has a paper that has recommendations for validation (standardization of IHC) for validation they want you to look at 25 different tissue samples, 10 of which are strong positive, 10 which are weak to moderately positive and 5 that are negative. In the case of prognostic and treatment related IHC they suggest that more tissues are evaluated. New lots require 3 tissues; strong positive, weak to moderate, and negative. Ultimately the your laboratory will come up with some system as how you are going to approach this. This is just a really basic overview and there is a lot more that can be added. In my opinion I think you need to do what makes sense in your laboratory. I would have a defined SOP for how you handle certain types of antibodies. Just my two cents. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, November 16, 2011 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Validation Just wondering what y'alls opinion is on validation: I don't really understand why optimization isn't enough. At that point, the pathologist has said what he wanted the stain to look like, so why do 3-10 positive slides on the new instrument to compare to previous slides from another instrument? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cls71877 <@t> sbcglobal.net Wed Nov 16 13:06:03 2011 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Wed Nov 16 13:06:06 2011 Subject: [Histonet] Paraplast X-tra Message-ID: <1321470363.98029.YahooMailRC@web81205.mail.mud.yahoo.com> Hello Histoland, We are seeing unusually large volumes of fall out in our paraffin lately.? The?precipitate is almost "muddy" or "oily" in appearance.??We noticed it immediately as it was melting, so there was no way anything else had been introduced to cause this.? As a result, we are seeing knife marks.? The marks are not consistent and not attributable to any of the tissue in the blocks.??We are blasting through blades that have been tried and true for years now.??Are there any other users of paraplast X-tra?experiencing these issues???? Thanks, Cristi From lpjones <@t> srhs-pa.org Wed Nov 16 13:07:23 2011 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Wed Nov 16 13:07:28 2011 Subject: [Histonet] Question For Anyone Using Soft LIS Message-ID: <4AE8039AEA096143B965CBC6D092166802351B3975@EXCH2007.srhs-pa.org> Greetings Histoland. We went live with Soft in September, and are still having issues finding a slide label/ribbon combination that doesn't fade or smear. Also, the slide labels currently being generated will not scan for our Pathologists. The products we are currently using are from a small, area company; and we are thinking it may be time to broaden our search to a larger company. Our printer is a Zebra GX430t. Thank you in advance! Laura ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From Timothy.Morken <@t> ucsfmedctr.org Wed Nov 16 13:08:31 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Nov 16 13:08:46 2011 Subject: [Histonet] RE: Validation In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC06303C@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BC06303C@JERRY.Gia.com> Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE4A3@MCINFRWEM003.ucsfmedicalcenter.org> Amber, is this new instrument the same kind as your old instrument and are you using the same reagents? Or is it a totally different platform with different reagents? Putting in a new instrument of the same kind and using the same reagents just requires verifying the new instrument works. You have to convince whoever wants to know that it works the same as the older instrument so running a variety of antibodies and procedures on it will suffice to prove that. Doing reproducibility tests also proves the instrument works as intended - 5 to 10 identical slides on one run, 5 to 10 identical slides on 5 to 10 runs. If it is a totally different platform with different reagents then you are in for revalidating each of your antibodies on the new system. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, November 16, 2011 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Validation Just wondering what y'alls opinion is on validation: I don't really understand why optimization isn't enough. At that point, the pathologist has said what he wanted the stain to look like, so why do 3-10 positive slides on the new instrument to compare to previous slides from another instrument? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Wed Nov 16 13:34:27 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Nov 16 13:34:37 2011 Subject: [Histonet] calmodulin Message-ID: Anyone had success getting Calmodulin up and running? I'm running in to some difficulties with an Abcam antibody one of our researchers gave me. Thanks for any assistance Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From contact <@t> excaliburpathology.com Wed Nov 16 13:36:36 2011 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Wed Nov 16 13:36:42 2011 Subject: [Histonet] Question For Anyone Using Soft LIS In-Reply-To: <4AE8039AEA096143B965CBC6D092166802351B3975@EXCH2007.srhs-pa.org> References: <4AE8039AEA096143B965CBC6D092166802351B3975@EXCH2007.srhs-pa.org> Message-ID: <1321472196.67578.YahooMailNeo@web1112.biz.mail.sk1.yahoo.com> Hi, ? I use labels and ribbons from this company. They do not wash off or fade. ? http://www.barcode-labels.com/solutions/laboratory-barcode-systems ? Labels part# 92169 and ribbon #T84252074 Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Jones, Laura" To: "Histonet@lists.utsouthwestern.edu" Sent: Wednesday, November 16, 2011 1:07 PM Subject: [Histonet] Question For Anyone Using Soft LIS Greetings Histoland.? We went live with Soft in September, and are still having issues finding a slide label/ribbon combination that doesn't fade or smear.? Also, the slide labels currently being generated will not scan for our Pathologists.? The products we are currently using are from a small, area company; and we are thinking it may be time to broaden our search to a larger company.? Our printer is a Zebra GX430t.? Thank you in advance!? Laura ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Nov 16 13:48:22 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Nov 16 13:48:27 2011 Subject: [Histonet] help In-Reply-To: <1300126180.1942476.1321448359281.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> References: <5C988DCFFE80014695B544D31BA8AA8A40FC9D73@000s-ex-mbx-qs1.unimelb.edu.au> <1300126180.1942476.1321448359281.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: <7490bfd7aa824.4ec3cd36@uwo.ca> The Turnbull's blue method as you describe it will not detect iron in tissues. Potassium ferricyanide will give a blue precipitate only with iron(II) (ferrous). In tissues, the iron is present as iron(III) (ferric) in such proteins as ferritin and haemosiderin. The iron of haemoglobin, though abundant, cannot be released by acid to react as Fe(II) or Fe(III) ions. The easiest way to detect Fe(III) is with potassium ferrocyanide - 0.05M in 0.2M HCl, for 30m - which gives a Prussian blue deposit (Perls' reaction). If you want to do a Turnbull's blue method, which some say is a bit more sensitive, you must first reduce all the Fe(III) in the tissue to Fe(II) with dilute ammonium sulphide. All the iron then ends up as precipitated FeS, which will react with an acidified potassium ferricyanide solution to produce Turnbull's blue. In fact, Prussian and Turnbull's blues are the same compound (see inorganic chemistry textbooks). A faint or invisible reaction product can be amplified because this pigment behaves like peroxidase, catalyzing the oxidation of DAB by H2O2 (see e.g. Connor et al. 1995). The sensitivity can be further increased with chemical tricks to change the brown oxidation product of DAB into larger quantities of black stuff (Moos & Mollgard 1993). References. Connor JR et 4 al (1995) A histochemical study of iron-positive cells in the developing rat brain. J. Comp. Neurol. 355:111-123. Moos T & Mollgard K (1993) A sensitive post-DAB enhancement technique for demonstration of iron in the central nervous system. Histochemistry 99:471-475. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Lydia Gunawan" To: Histonet@lists.utsouthwestern.edu Sent: Tuesday, November 15, 2011 2:00:32 PM Subject: [Histonet] help Hi there, I am having trouble with Turnbull staining. Anybody can help me? As I want to detect and quantify iron in the brain for Parkinson experiment, I am using mice(C57Bl6) brain that were treated with MPTP injection. So, I have been trying to stain those brain using paraffin section with Turnbull blue but I have no luck. FYI, I have been using 7% of Potassium ferricyanide in 3% HCL and I incubated for 2 hours, 37'. I also did incubation in triton-x and H2O2 too but still not getting any iron on my sections. Could anyone help me to solve my problem? Thanks Lydia Gunawan Oxidation Biology Laboratory Mental Health Research Institute Melbourne Brain Centre Corner Royal Pde and Genetics Lane University of Melbourne, Level 4 Parkville, Vic 3010 email: lydia.gunawan@unimelb.edu.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Wed Nov 16 13:54:11 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Nov 16 13:54:22 2011 Subject: [Histonet] Rb gene protein antibody Message-ID: Can someone recommend the best clone for Retinoblastoma Gene Protein on formalin-fixed paraffin sections? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From leticia.figliuolo <@t> roche.com Wed Nov 16 14:31:02 2011 From: leticia.figliuolo <@t> roche.com (Figliuolo, Leticia) Date: Wed Nov 16 14:31:31 2011 Subject: [Histonet] bar codes for slides and cassettes Message-ID: <069E6CF048B915488720412DAFD1474D041F86C90C@RNUMSEM702.nala.roche.com> Hello everybody, Is anybody out there using bar code system for slides/cassettes? My supervisor asked me to find out pros and cons and more information about this. I have no idea where to start... Thank you in advance! Leticia Figliuolo From mamawooo <@t> hotmail.com Wed Nov 16 14:45:45 2011 From: mamawooo <@t> hotmail.com (Janice Mahoney) Date: Wed Nov 16 14:45:49 2011 Subject: [Histonet] bar codes for slides and cassettes In-Reply-To: <069E6CF048B915488720412DAFD1474D041F86C90C@RNUMSEM702.nala.roche.com> References: <069E6CF048B915488720412DAFD1474D041F86C90C@RNUMSEM702.nala.roche.com> Message-ID: Leticia, We used Ventana Vantage in my lab and loved everything about it. It is so much more than just a labeling system. Check it out. It is pricy but worth every penny in what you will gain in productivity and error reduction. Jan Mahoney Omaha, NE > From: leticia.figliuolo@roche.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 16 Nov 2011 15:31:02 -0500 > Subject: [Histonet] bar codes for slides and cassettes > > Hello everybody, > > Is anybody out there using bar code system for slides/cassettes? > My supervisor asked me to find out pros and cons and more information about this. I have no idea where to start... > > Thank you in advance! > > Leticia Figliuolo > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Wed Nov 16 14:42:27 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Nov 16 14:49:40 2011 Subject: [Histonet] RE: Validation In-Reply-To: <8D7C2D242DBD45498006B21122072BF89F5EE4A3@MCINFRWEM003.ucsfmedicalcenter.org> References: <5A33C952BB67F4468AF1F36D739212BC06303C@JERRY.Gia.com> <8D7C2D242DBD45498006B21122072BF89F5EE4A3@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <1321476147.38937.YahooMailNeo@web112306.mail.gq1.yahoo.com> Seems the actual amount of validation slides can vary with different Medical directors( Pathologist) or thats what Ive seen in places. They( The Pathologist)?sign off on it. With that said ER/PR and Her2 have more vigorous criteria( very specific, such as the 25-50). Also, the 2011 AP133 guidline is suppose to go into more detail on this. I havnt seen that new recomendation yet. So If anyone has it, would love to see it posted here myself? ? Thanks ? Kim ________________________________ From: "Morken, Timothy" To: 'Amber McKenzie' ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, November 16, 2011 2:08 PM Subject: [Histonet] RE: Validation Amber, is this new instrument the same kind as your old instrument and are you using the same reagents? Or is it a totally different platform with different reagents? Putting in a new instrument of the same kind and using the same reagents just requires verifying the new instrument works. You have to convince whoever wants to know that it works the same as the older instrument so running a variety of antibodies and procedures on it will suffice to prove that. Doing reproducibility tests also proves the instrument works as intended - 5 to 10 identical slides on one run, 5 to 10 identical slides on 5 to 10 runs. If it is a totally different platform with different reagents then you are in for revalidating each of your antibodies on the new system. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, November 16, 2011 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Validation Just wondering what y'alls opinion is on validation:? I don't really understand why optimization isn't enough.? At that point, the pathologist has said what he wanted the stain to look like, so why do 3-10 positive slides on the new instrument to compare to previous slides from another instrument?? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Wed Nov 16 15:41:05 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Nov 16 15:41:08 2011 Subject: [Histonet] bar codes for slides and cassettes In-Reply-To: References: <069E6CF048B915488720412DAFD1474D041F86C90C@RNUMSEM702.nala.roche.com> Message-ID: <1321479665.56280.YahooMailNeo@web112306.mail.gq1.yahoo.com> Hi all, ? Dako also offers a labeling system. So does Cerner and Fisher diag. There are a few others out there too? ? Just thought you should know there are other less expensive systems out there that might provide you with what you are looking for thats not as expensive. It really depends on your specific needs. ? oh and Leticia, roche owns ventana and since your email is from there, I would start with them first :) ? Kim ? ? ________________________________ From: Janice Mahoney To: leticia.figliuolo@roche.com; histo net Sent: Wednesday, November 16, 2011 3:45 PM Subject: RE: [Histonet] bar codes for slides and cassettes Leticia, We used Ventana Vantage in my lab and loved everything about it.? It is so much more than just a labeling system.? Check it out.? It is pricy but worth every penny in what you will gain in productivity and error reduction. Jan Mahoney Omaha, NE > From: leticia.figliuolo@roche.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 16 Nov 2011 15:31:02 -0500 > Subject: [Histonet] bar codes for slides and cassettes > > Hello everybody, > > Is anybody out there using bar code system for slides/cassettes? > My supervisor asked me to find out pros and cons and more information about this. I have no idea where to start... > > Thank you in advance! > > Leticia Figliuolo > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronda.Souders <@t> meritushealth.com Wed Nov 16 14:28:24 2011 From: Ronda.Souders <@t> meritushealth.com (Ronda Souders) Date: Wed Nov 16 15:43:19 2011 Subject: [Histonet] QIHC Message-ID: Does anyone have any advice or know of any study guides for the QIHC test? Ronda Souders, HT (ASCP) Meritus Medical Lab Hagerstown, MD ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From one_angel_secret <@t> yahoo.com Wed Nov 16 15:47:28 2011 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Nov 16 15:47:33 2011 Subject: [Histonet] Paraplast X-tra In-Reply-To: <1321470363.98029.YahooMailRC@web81205.mail.mud.yahoo.com> References: <1321470363.98029.YahooMailRC@web81205.mail.mud.yahoo.com> Message-ID: <1321480048.30566.YahooMailNeo@web112302.mail.gq1.yahoo.com> Maybe you got a bad lot that is contaminated causing the knife marks? Also, Ive noticed that if you let your paraffin get to hot, it breaks down and that could be one reason for a consistency issue. Have you changed anything at all recently to your process? Hope this was helpful. ? Kim ________________________________ From: Cristi stephenson To: Histo Net Sent: Wednesday, November 16, 2011 2:06 PM Subject: [Histonet] Paraplast X-tra Hello Histoland, We are seeing unusually large volumes of fall out in our paraffin lately.? The?precipitate is almost "muddy" or "oily" in appearance.??We noticed it immediately as it was melting, so there was no way anything else had been introduced to cause this.? As a result, we are seeing knife marks.? The marks are not consistent and not attributable to any of the tissue in the blocks.??We are blasting through blades that have been tried and true for years now.??Are there any other users of paraplast X-tra?experiencing these issues???? Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Beth.Fye <@t> HCAhealthcare.com Wed Nov 16 16:15:57 2011 From: Beth.Fye <@t> HCAhealthcare.com (Beth.Fye@HCAhealthcare.com) Date: Wed Nov 16 16:16:07 2011 Subject: [Histonet] H. Pylori - IHC Message-ID: <938F8EC5A524D34EB5796E23E52781D36130C775FF@NADCWPMSGCMS05.hca.corpad.net> Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 From 41dmb41 <@t> gmail.com Wed Nov 16 16:20:21 2011 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Wed Nov 16 16:20:47 2011 Subject: [Histonet] H. Pylori - IHC In-Reply-To: <938F8EC5A524D34EB5796E23E52781D36130C775FF@NADCWPMSGCMS05.hca.corpad.net> References: <938F8EC5A524D34EB5796E23E52781D36130C775FF@NADCWPMSGCMS05.hca.corpad.net> Message-ID: We use the Rabbit Polyclonal H. pylori from Cell Marque on the Ventana Ultra's... We're a natinowide reference laboratory and our clients are all very happy with the specificty of the staining. Let me know if there's any more information you need. Drew On Wed, Nov 16, 2011 at 17:15, wrote: > Does anyone have recommendations for a good vendor for H.Pylori for IHC? > We have tried many, but our pathologists complain that they are not > specific for H.Pylori and that all gram negative bacteria are staining. > Currently we are not running this but our pathologists are interested in > it. > > Thanks! > > Beth A. Fye, CT (ASCP) > Pathology Technical Manager > HCA Richmond Hospital Laboratories > office: (804)228-6564 > fax: (804)323-8638 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From LSebree <@t> uwhealth.org Wed Nov 16 16:36:54 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Nov 16 16:36:57 2011 Subject: [Histonet] H. Pylori - IHC In-Reply-To: <938F8EC5A524D34EB5796E23E52781D36130C775FF@NADCWPMSGCMS05.hca.corpad.net> References: <938F8EC5A524D34EB5796E23E52781D36130C775FF@NADCWPMSGCMS05.hca.corpad.net> Message-ID: We use Ventana's, manufactured by Cell Marque. Most, if not all, of out pathologists have opted for this instead of histochemical staining for HP. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth.Fye@HCAhealthcare.com Sent: Wednesday, November 16, 2011 4:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori - IHC Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Nov 16 16:42:09 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Nov 16 16:42:25 2011 Subject: [Histonet] RE: Validation In-Reply-To: <1321476147.38937.YahooMailNeo@web112306.mail.gq1.yahoo.com> References: <5A33C952BB67F4468AF1F36D739212BC06303C@JERRY.Gia.com> <8D7C2D242DBD45498006B21122072BF89F5EE4A3@MCINFRWEM003.ucsfmedicalcenter.org> <1321476147.38937.YahooMailNeo@web112306.mail.gq1.yahoo.com> Message-ID: <8D7C2D242DBD45498006B21122072BF89F5EE4AB@MCINFRWEM003.ucsfmedicalcenter.org> Kim wrote: "Seems the actual amount of validation slides can vary with different Medical directors( Pathologist)" Yes, validation is simply the process of proving to yourself and anyone else (pathologist, clinician, inspector, patient, lawyer) that the product works as intended. Medical Directors are responsible for determining what that entails, whether large or small sets of tissue. There are certain recommendations that have been written up but it all comes down to being able to convince anyone who looks into it that everything is well done. CLIA regulations outline the process and following it will ensure you are on the right track. I have some documents up on a Yahoo groups page that anyone can download. They are from a validation presentation at NSH and ASCP meetings, including the presentation powerpoint outling CLIA and CAP requirements, model validation procedure and model validation documentation forms. You have to join the group but it is free and I promis there will be no emailings to bug you. Go to Yahool groups and search for "Histoinfo" group Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA ________________________________ From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Wednesday, November 16, 2011 12:42 PM To: Morken, Timothy; 'Amber McKenzie'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Validation Seems the actual amount of validation slides can vary with different Medical directors( Pathologist) or thats what Ive seen in places. They( The Pathologist) sign off on it. With that said ER/PR and Her2 have more vigorous criteria( very specific, such as the 25-50). Also, the 2011 AP133 guidline is suppose to go into more detail on this. I havnt seen that new recomendation yet. So If anyone has it, would love to see it posted here myself? Thanks Kim From: "Morken, Timothy" To: 'Amber McKenzie' ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, November 16, 2011 2:08 PM Subject: [Histonet] RE: Validation Amber, is this new instrument the same kind as your old instrument and are you using the same reagents? Or is it a totally different platform with different reagents? Putting in a new instrument of the same kind and using the same reagents just requires verifying the new instrument works. You have to convince whoever wants to know that it works the same as the older instrument so running a variety of antibodies and procedures on it will suffice to prove that. Doing reproducibility tests also proves the instrument works as intended - 5 to 10 identical slides on one run, 5 to 10 identical slides on 5 to 10 runs. If it is a totally different platform with different reagents then you are in for revalidating each of your antibodies on the new system. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, November 16, 2011 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Validation Just wondering what y'alls opinion is on validation: I don't really understand why optimization isn't enough. At that point, the pathologist has said what he wanted the stain to look like, so why do 3-10 positive slides on the new instrument to compare to previous slides from another instrument? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From itai.moshe <@t> mail.huji.ac.il Thu Nov 17 01:47:22 2011 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Thu Nov 17 01:47:31 2011 Subject: [Histonet] Paraffin slides storage Message-ID: Hello Histonets, I have some questions about paraffin slides storage. 1) For how long do you store paraffin slides after sectioning ? 2) At what conditions do you store the slides ? Itai From W.E.J.Hoekert <@t> olvg.nl Thu Nov 17 02:09:19 2011 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Thu Nov 17 02:11:49 2011 Subject: [Histonet] Fatty mamma tissue keeps on washing off References: <14E2C6176416974295479C64A11CB9AE1DECBA0C14@SBS2K8.premierlab.local> <1190CB05C44B13409483514729C2FC3601F841E0@PAIT42.olvg.nl> Message-ID: <1190CB05C44B13409483514729C2FC3601F841EC@PAIT42.olvg.nl> Hi histonetters Here is an update on my struggle to keep the fatty mamma tissue on the slides. I got a few responses on my mail so I have tried a few other things. Post fix the slides. Deparaffinize the slides using xylene and hydrate to water, post fix the slides in 10% NBF for 10 minutes (I did it for about 4 hours) and proceed as usual. That worked for a few of my hopeless cases. Still, some other cases would still come of the slides. I have also tried the Dako slides. Indeed they work great. Of the 4 cases that washed off no matter what, all but one stayed on the Dako slides. So from now on, if we have a case that washes off, we will be using the Dako slides. Thanks for your help, Willem Hoekert OLVG AMsterdam The Netherlands ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Hoekert, W.E.J. Verzonden: do 20-10-2011 12:09 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Fatty mamma tissue keeps on washing off Hi Histonetters, Every once in a while, we have mamma tissue that washes off the slides completely. The reason for this is that the tissue is still too fat and/or not sufficiently fixed. There is no problem with the HE stain, but the immuno will wash off. What do you do in such cases? I have tried the following: 1: Baking the slides for about 3/4 hours on superfrost slides (that works sometimes but not always) 2: Wood glue slides (that works sometimes but not always) 3:We put the blocks back in hot parraffin for several hours (that usually works) but I was told that this will have a bad influence on the Her2neu stain: a 2+ could become a 1+. I know, the solution is to be sure that the tissue is fixed long enough and that the fat has been removed sufficiently using acetone, but sometimes that is just not the case. How do you cope with this? Willem Hoekert PA-lab OLVG Amsterdam The Netherlands Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From SteveM <@t> mcclainlab.com Thu Nov 17 05:17:38 2011 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Thu Nov 17 05:18:23 2011 Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 24 reprocessing Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF137756@ML1.McClainLabs.local> This sounds like a complicated problem- Generally fixed tissues left in the processor overnight can just be re-hydrated in formalin for a few hours and then run (we've made that mistake) Exceptions abound in histology methods, but Unless the tissue was well-fixed to begin with, Re-processing generally stinks. Given that this is research and a one of a kind, it may still be worth trying. I would not expect Immunostains and other high-end work to be reliable even if you do get decent sections. First INVESTIGATE and determine what likely happened- Study the slides and see what is wrong -are they sunken and shriveled as if paraffin did not infiltrate? Second Study the cut surface of the blocks with a 10x lens or dissecting scope and determine if there was a processing failure also, any reason to suspect reagents needed changing? Your tissues sound maybe under-fixed (shrinkage described), probably dehydrated and then fixed by coagulation in the alcohols on the processor. Is it possible that the cleaning cycle was run before you started processing the next day? Third try Luna's method given by Shelly and do it manually and at room temp Fourth buy lunch for the investigator and meet to apologize and work out the details of fixation and any other issues discovered from your 'NTSB Accident Investigation'. Who knows, it may work. Fifth, assign one person to supervise (be in charge of) processing and have them keep statistics ( I have a large graph outside my door with 1 months data on each processor, graphing # of blocks for each run, when reagents were replenished/changed, noting every failure)- even in a small lab like ours, once we got to 6 processors running 1-4 times a day, someone needs to be in charge and held responsible. Six, hang a sign on the exit door (like the one on my back door) "DID YOU START THE TISSUE PROCESSOR?" Some may think that is over-reacting to a one-time error; when it happens a second time you earned and deserve the reputation. Be thankful it did not harm any patients- in the accident world that is known as a near-miss. Good luck Steve A. McClain, MD 631 361 4000 Message: 9 Date: Wed, 16 Nov 2011 15:05:38 +0000 From: Shelly Christenson Subject: RE: [Histonet] is there a way to recover ruined tissues To: Patsy Ruegg , 'histo net' Message-ID: <06E342B6098ED9478347E1407764C80404B13411@VETMXHT.ads.vet.k-state.edu> Content-Type: text/plain; charset="us-ascii" Patsy, I have used this reprocessing schedule from Lee Luna's book "Histopathologic Methods and Color Atlas of Specials Stains and Tissue Artifact". This procedure has worked for me on a couple of occasions, You may need to change the times a little since your tissue samples are so small. Luna's book has a lot of information that is very helpful, and would be a good reference book to have in the lab. Hope this helps you out, give it a try. Tissue Softening Solution The following solution is used for softening dried tissue specimens prior to reprocessing or processing Formol-Sodium Acetate (Stock) Solution Concentrated formalin (37%) solution 10 ml Sodium acetate 2 gm Tap water 90 ml Formol-Glycerol (Working) Solution Formalin-sodium acetate (stock) 90 ml Glycerol (glycerin) 10 ml Reprocessing Schedule Melt the paraffin blocks down Place the tissue in 3 changes of xylene for 1 hour each Place in 100% alcohol, 2 changes for 1 hour each Place in 95% alcohol, 2 changes for 1 hour each Place under running tap water for 20 minutes Place in formol-glycerol working solution until tissue becomes soft (In most instances this will occur within 5-8 hours: extended exposure to the formol-gycerol will not harm tissue specimens) Place the tissue in the process and proceed with routine Processing schedule. How ever it is not necessary for the tissue to go through the fixative stations on the processor Best of Luck, Shelly From SteveM <@t> mcclainlab.com Thu Nov 17 05:23:59 2011 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Thu Nov 17 05:24:37 2011 Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 24 reprocessing Other methods Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF13779E@ML1.McClainLabs.local> Not appropriate for your current mamafufu, But here are two 're-processing' methods used in our lab for small fixed tissues. Method 1 where the tissue was bit too large for a short processing cycle and the center of the block is sub-optimally infiltrated or soft and needs more paraffin time. Cutting can be improved by melting the block in a tissue mold, then change the paraffin and allow to sit for 30-60 minutes and re-embed in new paraffin. Method 2 Cleaning cycle method where small FIXED tissues were processed on too short a cycle, e.g., 4 mm punch biopsy on a 1 hour process (instead of a 4 -6 hour process) we have used this method for taking paraffin blocks back to alcohol. Melt blocks in correctly-sized molds in embedding center. Re-wrap tissues (carefully) and place back into cassettes and verify each lid is closed. Place in VIP-type processor with an automated cleaning cycle. Replace the purges with clean reagents. Run the cleaning cycle through the purge xylene-alcohol on the tissue processor, when completed- do not run through the water purge. Re-process (correctly) beginning in 95% alcohol. Steve A. McClain, MD 631 361 4000 From SteveM <@t> mcclainlab.com Thu Nov 17 05:39:32 2011 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Thu Nov 17 05:40:11 2011 Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 24 reprocessing Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF1377AD@ML1.McClainLabs.local> I just talked to Joel who reminded me. Mouse livers are often hard/ brittle, even under the best of circumstances. Avoid heat when processing, Room temp only Gradual dehydration 50/50/70/95% alc. 1 Hour per station. Steve 631 361 4000 From yesyes <@t> comcast.net Thu Nov 17 05:40:34 2011 From: yesyes <@t> comcast.net (yesyes@comcast.net) Date: Thu Nov 17 05:40:35 2011 Subject: [Histonet] broken chuck holder Message-ID: <1443769015.1841423.1321530034020.JavaMail.root@sz0175a.westchester.pa.mail.comcast.net> Does anyone have any experience with broken chuck holders? I have a tech that has snapped 3 chuck holders in little over two years. I have never seen this, has anyone else?? From David.Essex <@t> kingstonhospital.nhs.uk Thu Nov 17 05:42:37 2011 From: David.Essex <@t> kingstonhospital.nhs.uk (Essex, David) Date: Thu Nov 17 05:42:47 2011 Subject: [Histonet] broken chuck holder References: <20111117114143.6179744ABA7@nhs-pd1e-esg010.ad1.nhs.net> Message-ID: <20111117114238.813D1448F2B@nhs-pd1e-esg108.ad1.nhs.net> Only when I used to work with the Incredible Hulk..... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of yesyes@comcast.net Sent: 17 November 2011 11:41 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] broken chuck holder Does anyone have any experience with broken chuck holders? I have a tech that has snapped 3 chuck holders in little over two years. I have never seen this, has anyone else?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email is covered by the Kingston Hospital NHS Trust email disclaimer http://www.kingstonhospital.nhs.uk/trust-information/email-disclaimer From patpxs <@t> gmail.com Thu Nov 17 06:35:53 2011 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Nov 17 06:36:02 2011 Subject: [Histonet] broken chuck holder In-Reply-To: <20111117114238.813D1448F2B@nhs-pd1e-esg108.ad1.nhs.net> References: <20111117114143.6179744ABA7@nhs-pd1e-esg010.ad1.nhs.net> <20111117114238.813D1448F2B@nhs-pd1e-esg108.ad1.nhs.net> Message-ID: You should observe this tech to see what he/she is doing to break 3 in 2 years. Then you can have a training session in the proper use of the chuck. Either that or let them know that eating spinach before cutting is not a good idea.... On Thu, Nov 17, 2011 at 6:42 AM, Essex, David wrote: > Only when I used to work with the Incredible Hulk..... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > yesyes@comcast.net > Sent: 17 November 2011 11:41 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] broken chuck holder > > Does anyone have any experience with broken chuck holders? I have a tech > that has snapped 3 chuck holders in little over two years. I have never > seen this, has anyone else?? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This email is covered by the Kingston Hospital NHS Trust email disclaimer > http://www.kingstonhospital.nhs.uk/trust-information/email-disclaimer > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lblazek <@t> digestivespecialists.com Thu Nov 17 06:37:37 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Nov 17 06:37:41 2011 Subject: [Histonet] RE: H. Pylori - IHC In-Reply-To: <938F8EC5A524D34EB5796E23E52781D36130C775FF@NADCWPMSGCMS05.hca.corpad.net> References: <938F8EC5A524D34EB5796E23E52781D36130C775FF@NADCWPMSGCMS05.hca.corpad.net> Message-ID: <5A2BD13465E061429D6455C8D6B40E39137A4A0770@IBMB7Exchange.digestivespecialists.com> We use BioCare's H.Pylori for IHC and it is consistency is excellent. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth.Fye@HCAhealthcare.com Sent: Wednesday, November 16, 2011 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori - IHC Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Thu Nov 17 07:30:24 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Nov 17 07:30:33 2011 Subject: [Histonet] RE: H. Pylori - IHC In-Reply-To: <938F8EC5A524D34EB5796E23E52781D36130C775FF@NADCWPMSGCMS05.hca.corpad.net> References: <938F8EC5A524D34EB5796E23E52781D36130C775FF@NADCWPMSGCMS05.hca.corpad.net> Message-ID: We get beautiful staining with Dako's concentrate on the Benchmark XT. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth.Fye@HCAhealthcare.com Sent: Wednesday, November 16, 2011 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori - IHC Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From ihcman2010 <@t> hotmail.com Thu Nov 17 09:35:34 2011 From: ihcman2010 <@t> hotmail.com (Glen Dawson) Date: Thu Nov 17 09:35:44 2011 Subject: [Histonet] Manual or parts list for a Lipshaw autopsy table In-Reply-To: <1443769015.1841423.1321530034020.JavaMail.root@sz0175a.westchester.pa.mail.comcast.net> References: <1443769015.1841423.1321530034020.JavaMail.root@sz0175a.westchester.pa.mail.comcast.net> Message-ID: All, I was hoping that someone could help me locate an owner's manual or parts list for a Lipshaw Model LM-5-A Autopsy Table. It seems like the hydraulics are going out, but we need the manual to try to address the problem. Thanks In Advance, Glen Dawson BS, HT(ASCP) & QIHC Janesville, WI From sdysart <@t> mirnarx.com Thu Nov 17 10:20:53 2011 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Thu Nov 17 10:21:10 2011 Subject: [Histonet] IHC Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5001FC62@SN2PRD0702MB110.namprd07.prod.outlook.com> So...I am doing IHC stains with Caspase3 and Ki-67. Does anyone know a positive control for both of these at the same time? Will tonsil pop positive for both?? If not, what are good controls for each of them? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From liz <@t> premierlab.com Thu Nov 17 10:23:35 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Nov 17 10:23:39 2011 Subject: [Histonet] RE: IHC In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5001FC62@SN2PRD0702MB110.namprd07.prod.outlook.com> Message-ID: <14E2C6176416974295479C64A11CB9AE011380AC70D1@SBS2K8.premierlab.local> Tonsil is the control we use Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Thursday, November 17, 2011 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC So...I am doing IHC stains with Caspase3 and Ki-67. Does anyone know a positive control for both of these at the same time? Will tonsil pop positive for both?? If not, what are good controls for each of them? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Thu Nov 17 10:41:49 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Nov 17 10:40:50 2011 Subject: [Histonet] RE: Validation In-Reply-To: <8D7C2D242DBD45498006B21122072BF89F5EE4AB@MCINFRWEM003.ucsfmedicalcenter.org> References: <5A33C952BB67F4468AF1F36D739212BC06303C@JERRY.Gia.com> <8D7C2D242DBD45498006B21122072BF89F5EE4A3@MCINFRWEM003.ucsfmedicalcenter.org> <1321476147.38937.YahooMailNeo@web112306.mail.gq1.yahoo.com> <8D7C2D242DBD45498006B21122072BF89F5EE4AB@MCINFRWEM003.ucsfmedicalcenter.org> Message-ID: <5A33C952BB67F4468AF1F36D739212BC063249@JERRY.Gia.com> So can you do validation backwards? Stain the slides on the new instrument 1st and then stain on the old one to compare instead of looking up old cases to re-run on the new instrument? I have an XT (old) and Ultra (new). From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, November 16, 2011 4:42 PM To: 'Kim Donadio'; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Validation Kim wrote: "Seems the actual amount of validation slides can vary with different Medical directors( Pathologist)" Yes, validation is simply the process of proving to yourself and anyone else (pathologist, clinician, inspector, patient, lawyer) that the product works as intended. Medical Directors are responsible for determining what that entails, whether large or small sets of tissue. There are certain recommendations that have been written up but it all comes down to being able to convince anyone who looks into it that everything is well done. CLIA regulations outline the process and following it will ensure you are on the right track. I have some documents up on a Yahoo groups page that anyone can download. They are from a validation presentation at NSH and ASCP meetings, including the presentation powerpoint outling CLIA and CAP requirements, model validation procedure and model validation documentation forms. You have to join the group but it is free and I promis there will be no emailings to bug you. Go to Yahool groups and search for "Histoinfo" group Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA ________________________________ From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Wednesday, November 16, 2011 12:42 PM To: Morken, Timothy; 'Amber McKenzie'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Validation Seems the actual amount of validation slides can vary with different Medical directors( Pathologist) or thats what Ive seen in places. They( The Pathologist) sign off on it. With that said ER/PR and Her2 have more vigorous criteria( very specific, such as the 25-50). Also, the 2011 AP133 guidline is suppose to go into more detail on this. I havnt seen that new recomendation yet. So If anyone has it, would love to see it posted here myself? Thanks Kim From: "Morken, Timothy" To: 'Amber McKenzie' ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, November 16, 2011 2:08 PM Subject: [Histonet] RE: Validation Amber, is this new instrument the same kind as your old instrument and are you using the same reagents? Or is it a totally different platform with different reagents? Putting in a new instrument of the same kind and using the same reagents just requires verifying the new instrument works. You have to convince whoever wants to know that it works the same as the older instrument so running a variety of antibodies and procedures on it will suffice to prove that. Doing reproducibility tests also proves the instrument works as intended - 5 to 10 identical slides on one run, 5 to 10 identical slides on 5 to 10 runs. If it is a totally different platform with different reagents then you are in for revalidating each of your antibodies on the new system. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, November 16, 2011 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Validation Just wondering what y'alls opinion is on validation: I don't really understand why optimization isn't enough. At that point, the pathologist has said what he wanted the stain to look like, so why do 3-10 positive slides on the new instrument to compare to previous slides from another instrument? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Carol.Freeman <@t> utoledo.edu Thu Nov 17 12:14:58 2011 From: Carol.Freeman <@t> utoledo.edu (Freeman, Carol) Date: Thu Nov 17 12:15:20 2011 Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 26 In-Reply-To: References: Message-ID: In response to the broken chuck holders, I have seen it twice and both times it is with a tech who uses chemicals on blocks (RDO, FORMICAL or AMMONIA WATER ETC....) and then not rinse the blocks before putting them on the chuck holder. The chemicals erode the metal mechanism holding the spring and it will eventually snap. Have the tech rinse the blocks in water after taking them out of whatever chemical used before putting them on chuck holder and it should hopefully help. I hope that helps.... Carol E. Freeman HTL (ASCP) B.S. Department of Pathology University of Toledo Medical Center 3000 Arlington Avenue Toledo, OH 43614-5807 carol.freeman@utoledo.edu (419)383-5639 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, November 17, 2011 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 96, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Paraffin slides storage (Itai Moshe) 2. RE: Fatty mamma tissue keeps on washing off (Hoekert, W.E.J.) 3. RE: Histonet Digest, Vol 96, Issue 24 reprocessing (Steve McClain) 4. RE: Histonet Digest, Vol 96, Issue 24 reprocessing Other methods (Steve McClain) 5. RE: Histonet Digest, Vol 96, Issue 24 reprocessing (Steve McClain) 6. broken chuck holder (yesyes@comcast.net) 7. RE: broken chuck holder (Essex, David) 8. Re: broken chuck holder (Paula Sicurello) 9. RE: H. Pylori - IHC (Blazek, Linda) 10. RE: H. Pylori - IHC (Tom McNemar) 11. Manual or parts list for a Lipshaw autopsy table (Glen Dawson) 12. IHC (Sarah Dysart) 13. RE: IHC (Elizabeth Chlipala) 14. RE: RE: Validation (Amber McKenzie) ---------------------------------------------------------------------- Message: 1 Date: Thu, 17 Nov 2011 09:47:22 +0200 From: Itai Moshe Subject: [Histonet] Paraffin slides storage To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello Histonets, I have some questions about paraffin slides storage. 1) For how long do you store paraffin slides after sectioning ? 2) At what conditions do you store the slides ? Itai ------------------------------ Message: 2 Date: Thu, 17 Nov 2011 09:09:19 +0100 From: "Hoekert, W.E.J." Subject: RE: [Histonet] Fatty mamma tissue keeps on washing off To: "Hoekert, W.E.J." , Message-ID: <1190CB05C44B13409483514729C2FC3601F841EC@PAIT42.olvg.nl> Content-Type: text/plain; charset="iso-8859-1" Hi histonetters Here is an update on my struggle to keep the fatty mamma tissue on the slides. I got a few responses on my mail so I have tried a few other things. Post fix the slides. Deparaffinize the slides using xylene and hydrate to water, post fix the slides in 10% NBF for 10 minutes (I did it for about 4 hours) and proceed as usual. That worked for a few of my hopeless cases. Still, some other cases would still come of the slides. I have also tried the Dako slides. Indeed they work great. Of the 4 cases that washed off no matter what, all but one stayed on the Dako slides. So from now on, if we have a case that washes off, we will be using the Dako slides. Thanks for your help, Willem Hoekert OLVG AMsterdam The Netherlands ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Hoekert, W.E.J. Verzonden: do 20-10-2011 12:09 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Fatty mamma tissue keeps on washing off Hi Histonetters, Every once in a while, we have mamma tissue that washes off the slides completely. The reason for this is that the tissue is still too fat and/or not sufficiently fixed. There is no problem with the HE stain, but the immuno will wash off. What do you do in such cases? I have tried the following: 1: Baking the slides for about 3/4 hours on superfrost slides (that works sometimes but not always) 2: Wood glue slides (that works sometimes but not always) 3:We put the blocks back in hot parraffin for several hours (that usually works) but I was told that this will have a bad influence on the Her2neu stain: a 2+ could become a 1+. I know, the solution is to be sure that the tissue is fixed long enough and that the fat has been removed sufficiently using acetone, but sometimes that is just not the case. How do you cope with this? Willem Hoekert PA-lab OLVG Amsterdam The Netherlands Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ------------------------------ Message: 3 Date: Thu, 17 Nov 2011 11:17:38 +0000 From: Steve McClain Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 24 reprocessing To: "histonet@lists.utsouthwestern.edu" Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF137756@ML1.McClainLabs.local> Content-Type: text/plain; charset="us-ascii" This sounds like a complicated problem- Generally fixed tissues left in the processor overnight can just be re-hydrated in formalin for a few hours and then run (we've made that mistake) Exceptions abound in histology methods, but Unless the tissue was well-fixed to begin with, Re-processing generally stinks. Given that this is research and a one of a kind, it may still be worth trying. I would not expect Immunostains and other high-end work to be reliable even if you do get decent sections. First INVESTIGATE and determine what likely happened- Study the slides and see what is wrong -are they sunken and shriveled as if paraffin did not infiltrate? Second Study the cut surface of the blocks with a 10x lens or dissecting scope and determine if there was a processing failure also, any reason to suspect reagents needed changing? Your tissues sound maybe under-fixed (shrinkage described), probably dehydrated and then fixed by coagulation in the alcohols on the processor. Is it possible that the cleaning cycle was run before you started processing the next day? Third try Luna's method given by Shelly and do it manually and at room temp Fourth buy lunch for the investigator and meet to apologize and work out the details of fixation and any other issues discovered from your 'NTSB Accident Investigation'. Who knows, it may work. Fifth, assign one person to supervise (be in charge of) processing and have them keep statistics ( I have a large graph outside my door with 1 months data on each processor, graphing # of blocks for each run, when reagents were replenished/changed, noting every failure)- even in a small lab like ours, once we got to 6 processors running 1-4 times a day, someone needs to be in charge and held responsible. Six, hang a sign on the exit door (like the one on my back door) "DID YOU START THE TISSUE PROCESSOR?" Some may think that is over-reacting to a one-time error; when it happens a second time you earned and deserve the reputation. Be thankful it did not harm any patients- in the accident world that is known as a near-miss. Good luck Steve A. McClain, MD 631 361 4000 Message: 9 Date: Wed, 16 Nov 2011 15:05:38 +0000 From: Shelly Christenson Subject: RE: [Histonet] is there a way to recover ruined tissues To: Patsy Ruegg , 'histo net' Message-ID: <06E342B6098ED9478347E1407764C80404B13411@VETMXHT.ads.vet.k-state.edu> Content-Type: text/plain; charset="us-ascii" Patsy, I have used this reprocessing schedule from Lee Luna's book "Histopathologic Methods and Color Atlas of Specials Stains and Tissue Artifact". This procedure has worked for me on a couple of occasions, You may need to change the times a little since your tissue samples are so small. Luna's book has a lot of information that is very helpful, and would be a good reference book to have in the lab. Hope this helps you out, give it a try. Tissue Softening Solution The following solution is used for softening dried tissue specimens prior to reprocessing or processing Formol-Sodium Acetate (Stock) Solution Concentrated formalin (37%) solution 10 ml Sodium acetate 2 gm Tap water 90 ml Formol-Glycerol (Working) Solution Formalin-sodium acetate (stock) 90 ml Glycerol (glycerin) 10 ml Reprocessing Schedule Melt the paraffin blocks down Place the tissue in 3 changes of xylene for 1 hour each Place in 100% alcohol, 2 changes for 1 hour each Place in 95% alcohol, 2 changes for 1 hour each Place under running tap water for 20 minutes Place in formol-glycerol working solution until tissue becomes soft (In most instances this will occur within 5-8 hours: extended exposure to the formol-gycerol will not harm tissue specimens) Place the tissue in the process and proceed with routine Processing schedule. How ever it is not necessary for the tissue to go through the fixative stations on the processor Best of Luck, Shelly ------------------------------ Message: 4 Date: Thu, 17 Nov 2011 11:23:59 +0000 From: Steve McClain Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 24 reprocessing Other methods To: "histonet@lists.utsouthwestern.edu" Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF13779E@ML1.McClainLabs.local> Content-Type: text/plain; charset="us-ascii" Not appropriate for your current mamafufu, But here are two 're-processing' methods used in our lab for small fixed tissues. Method 1 where the tissue was bit too large for a short processing cycle and the center of the block is sub-optimally infiltrated or soft and needs more paraffin time. Cutting can be improved by melting the block in a tissue mold, then change the paraffin and allow to sit for 30-60 minutes and re-embed in new paraffin. Method 2 Cleaning cycle method where small FIXED tissues were processed on too short a cycle, e.g., 4 mm punch biopsy on a 1 hour process (instead of a 4 -6 hour process) we have used this method for taking paraffin blocks back to alcohol. Melt blocks in correctly-sized molds in embedding center. Re-wrap tissues (carefully) and place back into cassettes and verify each lid is closed. Place in VIP-type processor with an automated cleaning cycle. Replace the purges with clean reagents. Run the cleaning cycle through the purge xylene-alcohol on the tissue processor, when completed- do not run through the water purge. Re-process (correctly) beginning in 95% alcohol. Steve A. McClain, MD 631 361 4000 ------------------------------ Message: 5 Date: Thu, 17 Nov 2011 11:39:32 +0000 From: Steve McClain Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 24 reprocessing To: "histonet@lists.utsouthwestern.edu" Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF1377AD@ML1.McClainLabs.local> Content-Type: text/plain; charset="us-ascii" I just talked to Joel who reminded me. Mouse livers are often hard/ brittle, even under the best of circumstances. Avoid heat when processing, Room temp only Gradual dehydration 50/50/70/95% alc. 1 Hour per station. Steve 631 361 4000 ------------------------------ Message: 6 Date: Thu, 17 Nov 2011 11:40:34 +0000 (UTC) From: yesyes@comcast.net Subject: [Histonet] broken chuck holder To: histonet@lists.utsouthwestern.edu Message-ID: <1443769015.1841423.1321530034020.JavaMail.root@sz0175a.westchester.pa.m ail.comcast.net> Content-Type: text/plain; charset=utf-8 Does anyone have any experience with broken chuck holders? I have a tech that has snapped 3 chuck holders in little over two years. I have never seen this, has anyone else?? ------------------------------ Message: 7 Date: Thu, 17 Nov 2011 11:42:37 -0000 From: "Essex, David" Subject: RE: [Histonet] broken chuck holder To: , Message-ID: <20111117114238.813D1448F2B@nhs-pd1e-esg108.ad1.nhs.net> Content-Type: text/plain; charset="us-ascii" Only when I used to work with the Incredible Hulk..... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of yesyes@comcast.net Sent: 17 November 2011 11:41 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] broken chuck holder Does anyone have any experience with broken chuck holders? I have a tech that has snapped 3 chuck holders in little over two years. I have never seen this, has anyone else?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email is covered by the Kingston Hospital NHS Trust email disclaimer http://www.kingstonhospital.nhs.uk/trust-information/email-disclaimer ------------------------------ Message: 8 Date: Thu, 17 Nov 2011 07:35:53 -0500 From: Paula Sicurello Subject: Re: [Histonet] broken chuck holder To: "Essex, David" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 You should observe this tech to see what he/she is doing to break 3 in 2 years. Then you can have a training session in the proper use of the chuck. Either that or let them know that eating spinach before cutting is not a good idea.... On Thu, Nov 17, 2011 at 6:42 AM, Essex, David wrote: > Only when I used to work with the Incredible Hulk..... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > yesyes@comcast.net > Sent: 17 November 2011 11:41 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] broken chuck holder > > Does anyone have any experience with broken chuck holders? I have a tech > that has snapped 3 chuck holders in little over two years. I have never > seen this, has anyone else?? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This email is covered by the Kingston Hospital NHS Trust email disclaimer > http://www.kingstonhospital.nhs.uk/trust-information/email-disclaimer > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Thu, 17 Nov 2011 07:37:37 -0500 From: "Blazek, Linda" Subject: [Histonet] RE: H. Pylori - IHC To: "'Beth.Fye@HCAhealthcare.com'" , "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E39137A4A0770@IBMB7Exchange.digestivespeci alists.com> Content-Type: text/plain; charset="us-ascii" We use BioCare's H.Pylori for IHC and it is consistency is excellent. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth.Fye@HCAhealthcare.com Sent: Wednesday, November 16, 2011 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori - IHC Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 17 Nov 2011 08:30:24 -0500 From: Tom McNemar Subject: [Histonet] RE: H. Pylori - IHC To: "'Beth.Fye@HCAhealthcare.com'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We get beautiful staining with Dako's concentrate on the Benchmark XT. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth.Fye@HCAhealthcare.com Sent: Wednesday, November 16, 2011 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori - IHC Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 11 Date: Thu, 17 Nov 2011 09:35:34 -0600 From: Glen Dawson Subject: [Histonet] Manual or parts list for a Lipshaw autopsy table To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" All, I was hoping that someone could help me locate an owner's manual or parts list for a Lipshaw Model LM-5-A Autopsy Table. It seems like the hydraulics are going out, but we need the manual to try to address the problem. Thanks In Advance, Glen Dawson BS, HT(ASCP) & QIHC Janesville, WI ------------------------------ Message: 12 Date: Thu, 17 Nov 2011 16:20:53 +0000 From: Sarah Dysart Subject: [Histonet] IHC To: "histonet@lists.utsouthwestern.edu" Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5001FC62@SN2PRD0702MB110.namprd07.prod.ou tlook.com> Content-Type: text/plain; charset="us-ascii" So...I am doing IHC stains with Caspase3 and Ki-67. Does anyone know a positive control for both of these at the same time? Will tonsil pop positive for both?? If not, what are good controls for each of them? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ------------------------------ Message: 13 Date: Thu, 17 Nov 2011 09:23:35 -0700 From: Elizabeth Chlipala Subject: [Histonet] RE: IHC To: 'Sarah Dysart' , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE011380AC70D1@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Tonsil is the control we use Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Thursday, November 17, 2011 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC So...I am doing IHC stains with Caspase3 and Ki-67. Does anyone know a positive control for both of these at the same time? Will tonsil pop positive for both?? If not, what are good controls for each of them? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 17 Nov 2011 16:41:49 +0000 From: Amber McKenzie Subject: RE: [Histonet] RE: Validation To: "Morken, Timothy" , 'Kim Donadio' , "histonet@lists.utsouthwestern.edu" Message-ID: <5A33C952BB67F4468AF1F36D739212BC063249@JERRY.Gia.com> Content-Type: text/plain; charset="us-ascii" So can you do validation backwards? Stain the slides on the new instrument 1st and then stain on the old one to compare instead of looking up old cases to re-run on the new instrument? I have an XT (old) and Ultra (new). From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, November 16, 2011 4:42 PM To: 'Kim Donadio'; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Validation Kim wrote: "Seems the actual amount of validation slides can vary with different Medical directors( Pathologist)" Yes, validation is simply the process of proving to yourself and anyone else (pathologist, clinician, inspector, patient, lawyer) that the product works as intended. Medical Directors are responsible for determining what that entails, whether large or small sets of tissue. There are certain recommendations that have been written up but it all comes down to being able to convince anyone who looks into it that everything is well done. CLIA regulations outline the process and following it will ensure you are on the right track. I have some documents up on a Yahoo groups page that anyone can download. They are from a validation presentation at NSH and ASCP meetings, including the presentation powerpoint outling CLIA and CAP requirements, model validation procedure and model validation documentation forms. You have to join the group but it is free and I promis there will be no emailings to bug you. Go to Yahool groups and search for "Histoinfo" group Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA ________________________________ From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Wednesday, November 16, 2011 12:42 PM To: Morken, Timothy; 'Amber McKenzie'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Validation Seems the actual amount of validation slides can vary with different Medical directors( Pathologist) or thats what Ive seen in places. They( The Pathologist) sign off on it. With that said ER/PR and Her2 have more vigorous criteria( very specific, such as the 25-50). Also, the 2011 AP133 guidline is suppose to go into more detail on this. I havnt seen that new recomendation yet. So If anyone has it, would love to see it posted here myself? Thanks Kim From: "Morken, Timothy" To: 'Amber McKenzie' ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, November 16, 2011 2:08 PM Subject: [Histonet] RE: Validation Amber, is this new instrument the same kind as your old instrument and are you using the same reagents? Or is it a totally different platform with different reagents? Putting in a new instrument of the same kind and using the same reagents just requires verifying the new instrument works. You have to convince whoever wants to know that it works the same as the older instrument so running a variety of antibodies and procedures on it will suffice to prove that. Doing reproducibility tests also proves the instrument works as intended - 5 to 10 identical slides on one run, 5 to 10 identical slides on 5 to 10 runs. If it is a totally different platform with different reagents then you are in for revalidating each of your antibodies on the new system. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, November 16, 2011 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Validation Just wondering what y'alls opinion is on validation: I don't really understand why optimization isn't enough. At that point, the pathologist has said what he wanted the stain to look like, so why do 3-10 positive slides on the new instrument to compare to previous slides from another instrument? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 26 **************************************** From tmclaugh <@t> NEMOURS.ORG Thu Nov 17 13:24:46 2011 From: tmclaugh <@t> NEMOURS.ORG (McLaughlin, Terry ) Date: Thu Nov 17 13:25:37 2011 Subject: [Histonet] to cut or not to cut mouse brains Message-ID: <0B0238D44CE7C04E9D32455C8643D893E3FC0A@wlmmsx02.nemours.org> Hello All, I am having trouble cutting frozen mouse brains and was wondering if someone can offer some help. The mouse was perfused in 4% PFA , the brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose for 20 hrs, until the brain sank in this solution. It was frozen back by using a culture plate floating in liquid nitrogen with OCT . The problem I am having is many folds, and air bubbles under the tissue. What I have done so far: Cut at temp of -25, then tried - 22 and -17. Cut at 10um, then tried 16-25um. Tried making the knife colder by adding dry ice to it for a few seconds. Added dry ice to sample for a couple seconds. The sample cuts fine, it appears to happen when picking up on a slide. I tried picking up by starting at one side or end and letting tissue spread onto slide. Did not work. Also tried gently laying slide on top of sample and removing gently-still folds and air bubbles. Any help would be greatly appreciated as we have 6 more to do. Signed, Help! Terry Kokas From hfedor <@t> jhmi.edu Thu Nov 17 13:51:57 2011 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Nov 17 13:52:02 2011 Subject: [Histonet] RE: to cut or not to cut mouse brains In-Reply-To: <0B0238D44CE7C04E9D32455C8643D893E3FC0A@wlmmsx02.nemours.org> References: <0B0238D44CE7C04E9D32455C8643D893E3FC0A@wlmmsx02.nemours.org> Message-ID: Hello Terry, These are very difficult. The colder the better. (-27). keep the slides in the cryostat. Cut the section. Place section on a cold slide, and keep the slide in the croystat during the following procedure. Hold slide in left hand and brush in right. Place finger under one edge of the section and it will start to thaw, anchoring it to the slide. Gently brush out folds as you continue to advance the thaw line with your fingers under the slide. It takes a bit of practice, and not really perfect. But it works. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McLaughlin, Terry Sent: Thursday, November 17, 2011 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] to cut or not to cut mouse brains Hello All, I am having trouble cutting frozen mouse brains and was wondering if someone can offer some help. The mouse was perfused in 4% PFA , the brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose for 20 hrs, until the brain sank in this solution. It was frozen back by using a culture plate floating in liquid nitrogen with OCT . The problem I am having is many folds, and air bubbles under the tissue. What I have done so far: Cut at temp of -25, then tried - 22 and -17. Cut at 10um, then tried 16-25um. Tried making the knife colder by adding dry ice to it for a few seconds. Added dry ice to sample for a couple seconds. The sample cuts fine, it appears to happen when picking up on a slide. I tried picking up by starting at one side or end and letting tissue spread onto slide. Did not work. Also tried gently laying slide on top of sample and removing gently-still folds and air bubbles. Any help would be greatly appreciated as we have 6 more to do. Signed, Help! Terry Kokas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Nov 17 13:57:29 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Nov 17 13:57:41 2011 Subject: [Histonet] to cut or not to cut mouse brains In-Reply-To: <0B0238D44CE7C04E9D32455C8643D893E3FC0A@wlmmsx02.nemours.org> References: <0B0238D44CE7C04E9D32455C8643D893E3FC0A@wlmmsx02.nemours.org> Message-ID: I'm interested in this answer because this has happened to me - but not always. I usually cut brains at warmer temps. like -17 or -18 as opposed to -25 and they are usually handled in the same manner before being brought to the lab frozen in OCT. Andi On Nov 17, 2011, at 12:24 PM, McLaughlin, Terry wrote: > Hello All, > I am having trouble cutting frozen mouse brains and was wondering if > someone can offer some help. The mouse was perfused in 4% PFA , the > brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose > for 20 hrs, until the brain sank in this solution. It was frozen back by > using a culture plate floating in liquid nitrogen with OCT . > The problem I am having is many folds, and air bubbles under the tissue. > What I have done so far: > Cut at temp of -25, then tried - 22 and -17. > Cut at 10um, then tried 16-25um. > Tried making the knife colder by adding dry ice to it for a few seconds. > Added dry ice to sample for a couple seconds. > The sample cuts fine, it appears to happen when picking up on a slide. I > tried picking up by starting at one side or end and letting tissue > spread onto slide. Did not work. > Also tried gently laying slide on top of sample and removing > gently-still folds and air bubbles. Any help would be greatly > appreciated as we have 6 more to do. > > Signed, > Help! > Terry Kokas > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brett_connolly <@t> merck.com Thu Nov 17 13:58:19 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Nov 17 13:58:29 2011 Subject: [Histonet] RE: to cut or not to cut mouse brains In-Reply-To: <0B0238D44CE7C04E9D32455C8643D893E3FC0A@wlmmsx02.nemours.org> References: <0B0238D44CE7C04E9D32455C8643D893E3FC0A@wlmmsx02.nemours.org> Message-ID: Terry, Here what works for us - Slides are kept prechilled in a -20C freezer and then kept cold in the cryostat. Very gently press the cold slide onto the cut section so it makes contact and the section sticks to the slide. Flip slide over and use your finger to warm the back of the slide under the section. Starting with cold slides is a must for us. Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McLaughlin, Terry Sent: Thursday, November 17, 2011 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] to cut or not to cut mouse brains Hello All, I am having trouble cutting frozen mouse brains and was wondering if someone can offer some help. The mouse was perfused in 4% PFA , the brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose for 20 hrs, until the brain sank in this solution. It was frozen back by using a culture plate floating in liquid nitrogen with OCT . The problem I am having is many folds, and air bubbles under the tissue. What I have done so far: Cut at temp of -25, then tried - 22 and -17. Cut at 10um, then tried 16-25um. Tried making the knife colder by adding dry ice to it for a few seconds. Added dry ice to sample for a couple seconds. The sample cuts fine, it appears to happen when picking up on a slide. I tried picking up by starting at one side or end and letting tissue spread onto slide. Did not work. Also tried gently laying slide on top of sample and removing gently-still folds and air bubbles. Any help would be greatly appreciated as we have 6 more to do. Signed, Help! Terry Kokas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From brett_connolly <@t> merck.com Thu Nov 17 14:00:37 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Nov 17 14:00:42 2011 Subject: [Histonet] RE: to cut or not to cut mouse brains References: <0B0238D44CE7C04E9D32455C8643D893E3FC0A@wlmmsx02.nemours.org> Message-ID: Forgot to mention that we keep the cryostat around -16 to -18 C Brett -----Original Message----- From: Connolly, Brett M Sent: Thursday, November 17, 2011 2:58 PM To: 'McLaughlin, Terry '; histonet@lists.utsouthwestern.edu Subject: RE: to cut or not to cut mouse brains Terry, Here what works for us - Slides are kept prechilled in a -20C freezer and then kept cold in the cryostat. Very gently press the cold slide onto the cut section so it makes contact and the section sticks to the slide. Flip slide over and use your finger to warm the back of the slide under the section. Starting with cold slides is a must for us. Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McLaughlin, Terry Sent: Thursday, November 17, 2011 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] to cut or not to cut mouse brains Hello All, I am having trouble cutting frozen mouse brains and was wondering if someone can offer some help. The mouse was perfused in 4% PFA , the brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose for 20 hrs, until the brain sank in this solution. It was frozen back by using a culture plate floating in liquid nitrogen with OCT . The problem I am having is many folds, and air bubbles under the tissue. What I have done so far: Cut at temp of -25, then tried - 22 and -17. Cut at 10um, then tried 16-25um. Tried making the knife colder by adding dry ice to it for a few seconds. Added dry ice to sample for a couple seconds. The sample cuts fine, it appears to happen when picking up on a slide. I tried picking up by starting at one side or end and letting tissue spread onto slide. Did not work. Also tried gently laying slide on top of sample and removing gently-still folds and air bubbles. Any help would be greatly appreciated as we have 6 more to do. Signed, Help! Terry Kokas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From hfedor <@t> jhmi.edu Thu Nov 17 14:13:05 2011 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Nov 17 14:13:11 2011 Subject: [Histonet] to cut or not to cut mouse brains In-Reply-To: References: <0B0238D44CE7C04E9D32455C8643D893E3FC0A@wlmmsx02.nemours.org> Message-ID: When the blocks are not croyprotected you can cut them at warmer temperatures. But when they are cryoprotected with the 30% sucrose, they are soft at -18, that is why you need the colder temperature. they will compress at the warmer temperatures and give you a lot of wrinkles. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Thursday, November 17, 2011 2:57 PM To: McLaughlin, Terry Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] to cut or not to cut mouse brains I'm interested in this answer because this has happened to me - but not always. I usually cut brains at warmer temps. like -17 or -18 as opposed to -25 and they are usually handled in the same manner before being brought to the lab frozen in OCT. Andi On Nov 17, 2011, at 12:24 PM, McLaughlin, Terry wrote: > Hello All, > I am having trouble cutting frozen mouse brains and was wondering if > someone can offer some help. The mouse was perfused in 4% PFA , the > brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose > for 20 hrs, until the brain sank in this solution. It was frozen back > by using a culture plate floating in liquid nitrogen with OCT . > The problem I am having is many folds, and air bubbles under the tissue. > What I have done so far: > Cut at temp of -25, then tried - 22 and -17. > Cut at 10um, then tried 16-25um. > Tried making the knife colder by adding dry ice to it for a few seconds. > Added dry ice to sample for a couple seconds. > The sample cuts fine, it appears to happen when picking up on a slide. > I tried picking up by starting at one side or end and letting tissue > spread onto slide. Did not work. > Also tried gently laying slide on top of sample and removing > gently-still folds and air bubbles. Any help would be greatly > appreciated as we have 6 more to do. > > Signed, > Help! > Terry Kokas > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Jmoroch1 <@t> Fairview.org Thu Nov 17 14:34:55 2011 From: Jmoroch1 <@t> Fairview.org (Morocho, Jennifer) Date: Thu Nov 17 14:35:01 2011 Subject: [Histonet] HTL Lead Job Opening Message-ID: The University of MN Medical Center, Fairview has an exciting and immediate job opportunity as an HTL Technical Lead in Minneapolis, MN. Check out the Fairview web site www.fairview.org and look for job 11-36731. Or contact me, Jennifer Morocho, HTL (ASCP)CM at jmoroch1@fairview.org for more details. The Pathology Supervisor and I who would love to talk opportunity with you! From dgoodwin <@t> rwjuhh.edu Thu Nov 17 14:37:03 2011 From: dgoodwin <@t> rwjuhh.edu (Goodwin, Diana) Date: Thu Nov 17 14:37:07 2011 Subject: [Histonet] Brass cryostat chucks Message-ID: <09411E0112A96A459D8D5FBDAB9C15C72480DAE2DF@HAMEXMBA.rwjham.local> Greeting, Histonetters. I am in search of those brass cryostat chucks with the holes in them. Any ideas? Googled my brains out with no luck. Diana G. Goodwin, BS, HT(ASCP)QIHC Department of Pathology Robert Wood Johnson University Hospital at Hamilton One Hamilton Health Place Hamilton, NJ 08690 Ph: 609.631.6996 Email: dgoodwin@rwjuhh.edu From Sharron.Ladd <@t> immunogen.com Thu Nov 17 15:33:49 2011 From: Sharron.Ladd <@t> immunogen.com (Ladd, Sharron) Date: Thu Nov 17 15:33:43 2011 Subject: [Histonet] Source of IgG4 for Isotype control Message-ID: <1EC9E4133CB4EB4A90383226A986461404CB34A93E@wal-mail02> Hi Histonet, Where can I buy IgG4 in the US please? Thanks, Sharron From algranth <@t> email.arizona.edu Thu Nov 17 15:39:26 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Nov 17 15:39:35 2011 Subject: [Histonet] Brass cryostat chucks In-Reply-To: <09411E0112A96A459D8D5FBDAB9C15C72480DAE2DF@HAMEXMBA.rwjham.local> References: <09411E0112A96A459D8D5FBDAB9C15C72480DAE2DF@HAMEXMBA.rwjham.local> Message-ID: For what cryostat? On Nov 17, 2011, at 1:37 PM, Goodwin, Diana wrote: > Greeting, Histonetters. > > I am in search of those brass cryostat chucks with the holes in them. Any ideas? Googled my brains out with no luck. > > > Diana G. Goodwin, BS, HT(ASCP)QIHC > > Department of Pathology > > Robert Wood Johnson University Hospital at Hamilton > > One Hamilton Health Place > > Hamilton, NJ 08690 > > Ph: 609.631.6996 > > Email: dgoodwin@rwjuhh.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Christina.Wilson <@t> leica-microsystems.com Thu Nov 17 15:50:13 2011 From: Christina.Wilson <@t> leica-microsystems.com (Christina.Wilson@leica-microsystems.com) Date: Thu Nov 17 15:50:17 2011 Subject: [Histonet] AUTO: is out of the office. (returning Wed 10/19/2011) Message-ID: I am out of the office from Thu 11/17/2011 until Fri 11/18/2011. I will have limited access to emails during this time. If you should need assistance, please contact Demaris Mills, demaris.mills@leica-microsystems.com, for product management support or Karen Niewerth, karen.niewerth@leica-microsystems.com, for customer service support. Note: This is an automated response to your message "Histonet Digest, Vol 96, Issue 26" sent on 11/17/2011 12:02:06 PM. This is the only notification you will receive while this person is away. ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From ssegal2 <@t> slu.edu Thu Nov 17 16:02:50 2011 From: ssegal2 <@t> slu.edu (Salomao Segal) Date: Thu Nov 17 16:02:55 2011 Subject: [Histonet] microtomes Message-ID: The settings in a rotary microtome may indicate the thickness of sections but... how do you know that it indeed cuts at the indicated thickness, particularly if it is say an old device that you inherit from somebody else's lab junk? Is there a way of measuring the magnitude of advances after each rotation? Thanks Solomon Segal From tkngflght <@t> yahoo.com Thu Nov 17 17:09:21 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Nov 17 17:10:26 2011 Subject: [Histonet] broken chuck holder Message-ID: <1321571361.65679.YahooMailNeo@web39409.mail.mud.yahoo.com> Is your tech using anything like ammonia or soap on her ice to soak the blocks?? This would chew through the spring much?more quickly than just ice water...??? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From conniegrubaugh <@t> hotmail.com Thu Nov 17 17:46:16 2011 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Thu Nov 17 17:46:20 2011 Subject: [Histonet] Paraplast X-tra In-Reply-To: <1321480048.30566.YahooMailNeo@web112302.mail.gq1.yahoo.com> References: <1321470363.98029.YahooMailRC@web81205.mail.mud.yahoo.com>, <1321480048.30566.YahooMailNeo@web112302.mail.gq1.yahoo.com> Message-ID: We are having problems again too. Have not changed a thing and convinced that it is a bad lot again. Wonder sometimes on the QC that is done on the paraffin. Connie G. > Date: Wed, 16 Nov 2011 13:47:28 -0800 > From: one_angel_secret@yahoo.com > To: cls71877@sbcglobal.net; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Paraplast X-tra > CC: > > Maybe you got a bad lot that is contaminated causing the knife marks? Also, Ive noticed that if you let your paraffin get to hot, it breaks down and that could be one reason for a consistency issue. Have you changed anything at all recently to your process? Hope this was helpful. > > Kim > > > ________________________________ > From: Cristi stephenson > To: Histo Net > Sent: Wednesday, November 16, 2011 2:06 PM > Subject: [Histonet] Paraplast X-tra > > Hello Histoland, > We are seeing unusually large volumes of fall out in our paraffin lately. > The precipitate is almost "muddy" or "oily" in appearance. We noticed it > immediately as it was melting, so there was no way anything else had been > introduced to cause this. As a result, we are seeing knife marks. The marks > are not consistent and not attributable to any of the tissue in the blocks. We > are blasting through blades that have been tried and true for years now. Are > there any other users of paraplast X-tra experiencing these issues? > > Thanks, > Cristi > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cls71877 <@t> sbcglobal.net Thu Nov 17 18:08:33 2011 From: cls71877 <@t> sbcglobal.net (=?utf-8?B?Y2xzNzE4NzdAc2JjZ2xvYmFsLm5ldA==?=) Date: Thu Nov 17 18:08:23 2011 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gUGFyYXBsYXN0IFgtdHJh?= Message-ID: <74764.79442.bm@smtp214.mail.bf1.yahoo.com> I had to believe someone else out there was seeing this too!! Our rep contacted us today and is going to replace it and investigate...I too wonder how this gets past the QC dept., it is super obvious as soon as it starts melting.... Sent from my HTC on the Now Network from Sprint! ----- Reply message ----- From: "connie grubaugh" Date: Thu, Nov 17, 2011 3:46 pm Subject: [Histonet] Paraplast X-tra To: , , From karen <@t> gateslinger.com Thu Nov 17 21:32:04 2011 From: karen <@t> gateslinger.com (Karen Lahti) Date: Thu Nov 17 21:32:28 2011 Subject: [Histonet] RE: H. Pylori - IHC In-Reply-To: References: <938F8EC5A524D34EB5796E23E52781D36130C775FF@NADCWPMSGCMS05.hca.corpad.net> Message-ID: <007701cca5a2$a4e88a90$eeb99fb0$@com> We use Biocare antibody with great results on the IntelliPath from Biocare and the Bondmax from Leica. Karen Lahti Arizona Digestive Health -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, November 17, 2011 6:30 AM To: 'Beth.Fye@HCAhealthcare.com'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: H. Pylori - IHC We get beautiful staining with Dako's concentrate on the Benchmark XT. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth.Fye@HCAhealthcare.com Sent: Wednesday, November 16, 2011 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori - IHC Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Nov 18 07:14:54 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Nov 18 07:16:39 2011 Subject: [Histonet] RE: Source of IgG4 for Isotype control In-Reply-To: <1EC9E4133CB4EB4A90383226A986461404CB34A93E@wal-mail02> References: <1EC9E4133CB4EB4A90383226A986461404CB34A93E@wal-mail02> Message-ID: Cell Marque 0.1 ml concentrated 367M-14 0.5 ml concentrated 367M-15 1 ml concentrated 367M-16 1 ml prediluted 367M-17 7 ml prediluted 367M-18 We use it routinely and it works great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ladd, Sharron [Sharron.Ladd@immunogen.com] Sent: Thursday, November 17, 2011 4:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Source of IgG4 for Isotype control Hi Histonet, Where can I buy IgG4 in the US please? Thanks, Sharron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sharron.Ladd <@t> immunogen.com Fri Nov 18 07:22:05 2011 From: Sharron.Ladd <@t> immunogen.com (Ladd, Sharron) Date: Fri Nov 18 07:21:57 2011 Subject: [Histonet] RE: Source of IgG4 for Isotype control In-Reply-To: References: <1EC9E4133CB4EB4A90383226A986461404CB34A93E@wal-mail02> Message-ID: <1EC9E4133CB4EB4A90383226A986461404CB34A944@wal-mail02> Sorry I should have been more specific. I am not looking for a mouse monoclonal anti-human IgG4 antibody. I need human IgG4 to run as an isotype control for another primary mouse monoclonal that is an IgG4 isotype. The only company I have found so far that sell purified Human IgG4 is in England and the shipping time is too long. Happy Friday! Sharron -----Original Message----- From: McMahon, Loralee A [mailto:Loralee_Mcmahon@URMC.Rochester.edu] Sent: Friday, November 18, 2011 8:15 AM To: Ladd, Sharron; 'histonet@lists.utsouthwestern.edu' Subject: RE: Source of IgG4 for Isotype control Cell Marque 0.1 ml concentrated 367M-14 0.5 ml concentrated 367M-15 1 ml concentrated 367M-16 1 ml prediluted 367M-17 7 ml prediluted 367M-18 We use it routinely and it works great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ladd, Sharron [Sharron.Ladd@immunogen.com] Sent: Thursday, November 17, 2011 4:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Source of IgG4 for Isotype control Hi Histonet, Where can I buy IgG4 in the US please? Thanks, Sharron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Fri Nov 18 07:55:01 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Nov 18 07:55:10 2011 Subject: [Histonet] microtomes Message-ID: Hi, If you are concerned about the thickness of the sections being accurate to the setting, I would suggest picking up a cheap micrometer from a hardware store. (OK perhaps not cheap as you will want a quality one, but the cost of these isn't terrible.) You can't really measure 5 microns (or whatever you cut at) well, so you will need to take 10-50 sections of a blank block. Remember to keep a constant rhythm that you would use on a normal block. Measure the thickness before and after then divide these thicknesses by the number of sections taken. Remember, also, to take the measurements at the same temperature that you are cutting the sections at. If your difference isn't exactly right how far off is it? You would then know if you should use a higher or lower setting on the microtome. Amos On Thu, Nov 17, 2011 at 10:33 PM, wrote: > Message: 13 > Date: Thu, 17 Nov 2011 16:02:50 -0600 > From: Salomao Segal > Subject: [Histonet] microtomes > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=ISO-8859-1 > > The settings in a rotary microtome may indicate the thickness of sections > but... > > how do you know that it indeed cuts at the indicated thickness, > particularly if it is say an old device that you inherit from somebody > else's lab junk? > > Is there a way of measuring the magnitude of advances after each rotation? > > Thanks > > > Solomon Segal > From wdesalvo.cac <@t> hotmail.com Fri Nov 18 08:33:30 2011 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Fri Nov 18 08:33:38 2011 Subject: [Histonet] RE: Source of IgG4 for Isotype control In-Reply-To: <1EC9E4133CB4EB4A90383226A986461404CB34A944@wal-mail02> References: <1EC9E4133CB4EB4A90383226A986461404CB34A93E@wal-mail02>, , <1EC9E4133CB4EB4A90383226A986461404CB34A944@wal-mail02> Message-ID: First try Sigma Aldrich William DeSalvo, B.S., HTL(ASCP) > From: Sharron.Ladd@immunogen.com > To: Loralee_Mcmahon@URMC.Rochester.edu; histonet@lists.utsouthwestern.edu > Date: Fri, 18 Nov 2011 08:22:05 -0500 > CC: > Subject: [Histonet] RE: Source of IgG4 for Isotype control > > Sorry I should have been more specific. I am not looking for a mouse monoclonal anti-human IgG4 antibody. I need human IgG4 to run as an isotype control for another primary mouse monoclonal that is an IgG4 isotype. The only company I have found so far that sell purified Human IgG4 is in England and the shipping time is too long. > Happy Friday! > Sharron > > -----Original Message----- > From: McMahon, Loralee A [mailto:Loralee_Mcmahon@URMC.Rochester.edu] > Sent: Friday, November 18, 2011 8:15 AM > To: Ladd, Sharron; 'histonet@lists.utsouthwestern.edu' > Subject: RE: Source of IgG4 for Isotype control > > Cell Marque > > 0.1 ml concentrated 367M-14 > 0.5 ml concentrated 367M-15 > 1 ml concentrated 367M-16 > 1 ml prediluted 367M-17 > 7 ml prediluted 367M-18 > > We use it routinely and it works great. > > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ladd, Sharron [Sharron.Ladd@immunogen.com] > Sent: Thursday, November 17, 2011 4:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Source of IgG4 for Isotype control > > Hi Histonet, > Where can I buy IgG4 in the US please? > Thanks, > Sharron > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Fri Nov 18 09:00:31 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Nov 18 09:00:46 2011 Subject: [Histonet] Antibodies for hamster tissue Message-ID: Hi all , Wondering if anyone has experience with these antibodies on hamster tissues: - Cathepsin B or a pan-cathepsin Ab if on exists - MMP-3, MMP9, MMP12 - neutrophil elastase Thanks for any info. Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From mhale <@t> carisls.com Fri Nov 18 09:14:43 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Fri Nov 18 09:14:57 2011 Subject: [Histonet] Great Position in AZ Message-ID: <6F33D8418806044682A391273399860F0AA1DEC1@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in Scottsdale , AZ . Colon and Digestive Health Specialists is looking for a certified HT or HTL to run their laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours up to 25 hours a week . Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From mhale <@t> carisls.com Fri Nov 18 09:15:28 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Fri Nov 18 09:15:38 2011 Subject: [Histonet] Great Position in Louisiana Message-ID: <6F33D8418806044682A391273399860F0AA1DEC6@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice located in Lafayette, Louisiana, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From shive003 <@t> umn.edu Fri Nov 18 09:21:39 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Nov 18 09:21:46 2011 Subject: [Histonet] mouse xGFAP on rabbit Message-ID: Good Morning, Does anyone know of a *mouse* xGFAP that will work on rabbit tissue? Thanks in advance, -- Jan Shivers Senior Scientist IHC/Histology/EM Section Head Pathology Teaching Program UMN Veterinary Diagnostic Laboratory University of Minnesota College of Veterinary Medicine 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From mhale <@t> carisls.com Fri Nov 18 09:28:59 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Fri Nov 18 09:29:11 2011 Subject: [Histonet] Great Position in AZ Message-ID: <6F33D8418806044682A391273399860F0AA1DF13@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in Scottsdale , AZ . Colon and Digestive Health Specialists is looking for a certified HT or HTL to run their laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours up to 25 hours a week . Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From Paul <@t> Firnschild.com Fri Nov 18 10:10:09 2011 From: Paul <@t> Firnschild.com (Paul Firnschild) Date: Fri Nov 18 10:10:20 2011 Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 27 In-Reply-To: <1A.1C.18304.353D5CE4@cm-mr6> References: <1A.1C.18304.353D5CE4@cm-mr6> Message-ID: <000701cca60c$8c685d50$a53917f0$@com> Solomon: Regarding your question of how to know if the microtome advance amount. Verifying this measurement is part of the service I provide as a service technician. I typically do this once of twice a year as part of the routine microtome maintenance service. Please contact me if I can be of service to you. Paul M. Firnschild QA Support Services, Inc. (404) 291.3715 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, November 17, 2011 10:39 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 96, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Histonet Digest, Vol 96, Issue 26 (Freeman, Carol) 2. to cut or not to cut mouse brains (McLaughlin, Terry ) 3. RE: to cut or not to cut mouse brains (Helen Fedor) 4. Re: to cut or not to cut mouse brains (Grantham, Andrea L - (algranth)) 5. RE: to cut or not to cut mouse brains (Connolly, Brett M) 6. RE: to cut or not to cut mouse brains (Connolly, Brett M) 7. RE: to cut or not to cut mouse brains (Helen Fedor) 8. HTL Lead Job Opening (Morocho, Jennifer) 9. Brass cryostat chucks (Goodwin, Diana) 10. Source of IgG4 for Isotype control (Ladd, Sharron) 11. Re: Brass cryostat chucks (Grantham, Andrea L - (algranth)) 12. AUTO: is out of the office. (returning Wed 10/19/2011) (Christina.Wilson@leica-microsystems.com) 13. microtomes (Salomao Segal) 14. broken chuck holder (Cheryl) 15. RE: Paraplast X-tra (connie grubaugh) 16. Re: Paraplast X-tra ( cls71877@sbcglobal.net ) 17. RE: RE: H. Pylori - IHC (Karen Lahti) ---------------------------------------------------------------------- Message: 1 Date: Thu, 17 Nov 2011 13:14:58 -0500 From: "Freeman, Carol" Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 26 To: Message-ID: Content-Type: text/plain; charset="us-ascii" In response to the broken chuck holders, I have seen it twice and both times it is with a tech who uses chemicals on blocks (RDO, FORMICAL or AMMONIA WATER ETC....) and then not rinse the blocks before putting them on the chuck holder. The chemicals erode the metal mechanism holding the spring and it will eventually snap. Have the tech rinse the blocks in water after taking them out of whatever chemical used before putting them on chuck holder and it should hopefully help. I hope that helps.... Carol E. Freeman HTL (ASCP) B.S. Department of Pathology University of Toledo Medical Center 3000 Arlington Avenue Toledo, OH 43614-5807 carol.freeman@utoledo.edu (419)383-5639 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, November 17, 2011 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 96, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Paraffin slides storage (Itai Moshe) 2. RE: Fatty mamma tissue keeps on washing off (Hoekert, W.E.J.) 3. RE: Histonet Digest, Vol 96, Issue 24 reprocessing (Steve McClain) 4. RE: Histonet Digest, Vol 96, Issue 24 reprocessing Other methods (Steve McClain) 5. RE: Histonet Digest, Vol 96, Issue 24 reprocessing (Steve McClain) 6. broken chuck holder (yesyes@comcast.net) 7. RE: broken chuck holder (Essex, David) 8. Re: broken chuck holder (Paula Sicurello) 9. RE: H. Pylori - IHC (Blazek, Linda) 10. RE: H. Pylori - IHC (Tom McNemar) 11. Manual or parts list for a Lipshaw autopsy table (Glen Dawson) 12. IHC (Sarah Dysart) 13. RE: IHC (Elizabeth Chlipala) 14. RE: RE: Validation (Amber McKenzie) ---------------------------------------------------------------------- Message: 1 Date: Thu, 17 Nov 2011 09:47:22 +0200 From: Itai Moshe Subject: [Histonet] Paraffin slides storage To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello Histonets, I have some questions about paraffin slides storage. 1) For how long do you store paraffin slides after sectioning ? 2) At what conditions do you store the slides ? Itai ------------------------------ Message: 2 Date: Thu, 17 Nov 2011 09:09:19 +0100 From: "Hoekert, W.E.J." Subject: RE: [Histonet] Fatty mamma tissue keeps on washing off To: "Hoekert, W.E.J." , Message-ID: <1190CB05C44B13409483514729C2FC3601F841EC@PAIT42.olvg.nl> Content-Type: text/plain; charset="iso-8859-1" Hi histonetters Here is an update on my struggle to keep the fatty mamma tissue on the slides. I got a few responses on my mail so I have tried a few other things. Post fix the slides. Deparaffinize the slides using xylene and hydrate to water, post fix the slides in 10% NBF for 10 minutes (I did it for about 4 hours) and proceed as usual. That worked for a few of my hopeless cases. Still, some other cases would still come of the slides. I have also tried the Dako slides. Indeed they work great. Of the 4 cases that washed off no matter what, all but one stayed on the Dako slides. So from now on, if we have a case that washes off, we will be using the Dako slides. Thanks for your help, Willem Hoekert OLVG AMsterdam The Netherlands ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Hoekert, W.E.J. Verzonden: do 20-10-2011 12:09 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Fatty mamma tissue keeps on washing off Hi Histonetters, Every once in a while, we have mamma tissue that washes off the slides completely. The reason for this is that the tissue is still too fat and/or not sufficiently fixed. There is no problem with the HE stain, but the immuno will wash off. What do you do in such cases? I have tried the following: 1: Baking the slides for about 3/4 hours on superfrost slides (that works sometimes but not always) 2: Wood glue slides (that works sometimes but not always) 3:We put the blocks back in hot parraffin for several hours (that usually works) but I was told that this will have a bad influence on the Her2neu stain: a 2+ could become a 1+. I know, the solution is to be sure that the tissue is fixed long enough and that the fat has been removed sufficiently using acetone, but sometimes that is just not the case. How do you cope with this? Willem Hoekert PA-lab OLVG Amsterdam The Netherlands Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ------------------------------ Message: 3 Date: Thu, 17 Nov 2011 11:17:38 +0000 From: Steve McClain Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 24 reprocessing To: "histonet@lists.utsouthwestern.edu" Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF137756@ML1.McClainLabs.local> Content-Type: text/plain; charset="us-ascii" This sounds like a complicated problem- Generally fixed tissues left in the processor overnight can just be re-hydrated in formalin for a few hours and then run (we've made that mistake) Exceptions abound in histology methods, but Unless the tissue was well-fixed to begin with, Re-processing generally stinks. Given that this is research and a one of a kind, it may still be worth trying. I would not expect Immunostains and other high-end work to be reliable even if you do get decent sections. First INVESTIGATE and determine what likely happened- Study the slides and see what is wrong -are they sunken and shriveled as if paraffin did not infiltrate? Second Study the cut surface of the blocks with a 10x lens or dissecting scope and determine if there was a processing failure also, any reason to suspect reagents needed changing? Your tissues sound maybe under-fixed (shrinkage described), probably dehydrated and then fixed by coagulation in the alcohols on the processor. Is it possible that the cleaning cycle was run before you started processing the next day? Third try Luna's method given by Shelly and do it manually and at room temp Fourth buy lunch for the investigator and meet to apologize and work out the details of fixation and any other issues discovered from your 'NTSB Accident Investigation'. Who knows, it may work. Fifth, assign one person to supervise (be in charge of) processing and have them keep statistics ( I have a large graph outside my door with 1 months data on each processor, graphing # of blocks for each run, when reagents were replenished/changed, noting every failure)- even in a small lab like ours, once we got to 6 processors running 1-4 times a day, someone needs to be in charge and held responsible. Six, hang a sign on the exit door (like the one on my back door) "DID YOU START THE TISSUE PROCESSOR?" Some may think that is over-reacting to a one-time error; when it happens a second time you earned and deserve the reputation. Be thankful it did not harm any patients- in the accident world that is known as a near-miss. Good luck Steve A. McClain, MD 631 361 4000 Message: 9 Date: Wed, 16 Nov 2011 15:05:38 +0000 From: Shelly Christenson Subject: RE: [Histonet] is there a way to recover ruined tissues To: Patsy Ruegg , 'histo net' Message-ID: <06E342B6098ED9478347E1407764C80404B13411@VETMXHT.ads.vet.k-state.edu> Content-Type: text/plain; charset="us-ascii" Patsy, I have used this reprocessing schedule from Lee Luna's book "Histopathologic Methods and Color Atlas of Specials Stains and Tissue Artifact". This procedure has worked for me on a couple of occasions, You may need to change the times a little since your tissue samples are so small. Luna's book has a lot of information that is very helpful, and would be a good reference book to have in the lab. Hope this helps you out, give it a try. Tissue Softening Solution The following solution is used for softening dried tissue specimens prior to reprocessing or processing Formol-Sodium Acetate (Stock) Solution Concentrated formalin (37%) solution 10 ml Sodium acetate 2 gm Tap water 90 ml Formol-Glycerol (Working) Solution Formalin-sodium acetate (stock) 90 ml Glycerol (glycerin) 10 ml Reprocessing Schedule Melt the paraffin blocks down Place the tissue in 3 changes of xylene for 1 hour each Place in 100% alcohol, 2 changes for 1 hour each Place in 95% alcohol, 2 changes for 1 hour each Place under running tap water for 20 minutes Place in formol-glycerol working solution until tissue becomes soft (In most instances this will occur within 5-8 hours: extended exposure to the formol-gycerol will not harm tissue specimens) Place the tissue in the process and proceed with routine Processing schedule. How ever it is not necessary for the tissue to go through the fixative stations on the processor Best of Luck, Shelly ------------------------------ Message: 4 Date: Thu, 17 Nov 2011 11:23:59 +0000 From: Steve McClain Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 24 reprocessing Other methods To: "histonet@lists.utsouthwestern.edu" Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF13779E@ML1.McClainLabs.local> Content-Type: text/plain; charset="us-ascii" Not appropriate for your current mamafufu, But here are two 're-processing' methods used in our lab for small fixed tissues. Method 1 where the tissue was bit too large for a short processing cycle and the center of the block is sub-optimally infiltrated or soft and needs more paraffin time. Cutting can be improved by melting the block in a tissue mold, then change the paraffin and allow to sit for 30-60 minutes and re-embed in new paraffin. Method 2 Cleaning cycle method where small FIXED tissues were processed on too short a cycle, e.g., 4 mm punch biopsy on a 1 hour process (instead of a 4 -6 hour process) we have used this method for taking paraffin blocks back to alcohol. Melt blocks in correctly-sized molds in embedding center. Re-wrap tissues (carefully) and place back into cassettes and verify each lid is closed. Place in VIP-type processor with an automated cleaning cycle. Replace the purges with clean reagents. Run the cleaning cycle through the purge xylene-alcohol on the tissue processor, when completed- do not run through the water purge. Re-process (correctly) beginning in 95% alcohol. Steve A. McClain, MD 631 361 4000 ------------------------------ Message: 5 Date: Thu, 17 Nov 2011 11:39:32 +0000 From: Steve McClain Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 24 reprocessing To: "histonet@lists.utsouthwestern.edu" Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF1377AD@ML1.McClainLabs.local> Content-Type: text/plain; charset="us-ascii" I just talked to Joel who reminded me. Mouse livers are often hard/ brittle, even under the best of circumstances. Avoid heat when processing, Room temp only Gradual dehydration 50/50/70/95% alc. 1 Hour per station. Steve 631 361 4000 ------------------------------ Message: 6 Date: Thu, 17 Nov 2011 11:40:34 +0000 (UTC) From: yesyes@comcast.net Subject: [Histonet] broken chuck holder To: histonet@lists.utsouthwestern.edu Message-ID: <1443769015.1841423.1321530034020.JavaMail.root@sz0175a.westchester.pa.m ail.comcast.net> Content-Type: text/plain; charset=utf-8 Does anyone have any experience with broken chuck holders? I have a tech that has snapped 3 chuck holders in little over two years. I have never seen this, has anyone else?? ------------------------------ Message: 7 Date: Thu, 17 Nov 2011 11:42:37 -0000 From: "Essex, David" Subject: RE: [Histonet] broken chuck holder To: , Message-ID: <20111117114238.813D1448F2B@nhs-pd1e-esg108.ad1.nhs.net> Content-Type: text/plain; charset="us-ascii" Only when I used to work with the Incredible Hulk..... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of yesyes@comcast.net Sent: 17 November 2011 11:41 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] broken chuck holder Does anyone have any experience with broken chuck holders? I have a tech that has snapped 3 chuck holders in little over two years. I have never seen this, has anyone else?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email is covered by the Kingston Hospital NHS Trust email disclaimer http://www.kingstonhospital.nhs.uk/trust-information/email-disclaimer ------------------------------ Message: 8 Date: Thu, 17 Nov 2011 07:35:53 -0500 From: Paula Sicurello Subject: Re: [Histonet] broken chuck holder To: "Essex, David" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 You should observe this tech to see what he/she is doing to break 3 in 2 years. Then you can have a training session in the proper use of the chuck. Either that or let them know that eating spinach before cutting is not a good idea.... On Thu, Nov 17, 2011 at 6:42 AM, Essex, David wrote: > Only when I used to work with the Incredible Hulk..... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > yesyes@comcast.net > Sent: 17 November 2011 11:41 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] broken chuck holder > > Does anyone have any experience with broken chuck holders? I have a tech > that has snapped 3 chuck holders in little over two years. I have never > seen this, has anyone else?? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This email is covered by the Kingston Hospital NHS Trust email disclaimer > http://www.kingstonhospital.nhs.uk/trust-information/email-disclaimer > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Thu, 17 Nov 2011 07:37:37 -0500 From: "Blazek, Linda" Subject: [Histonet] RE: H. Pylori - IHC To: "'Beth.Fye@HCAhealthcare.com'" , "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E39137A4A0770@IBMB7Exchange.digestivespeci alists.com> Content-Type: text/plain; charset="us-ascii" We use BioCare's H.Pylori for IHC and it is consistency is excellent. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth.Fye@HCAhealthcare.com Sent: Wednesday, November 16, 2011 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori - IHC Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 17 Nov 2011 08:30:24 -0500 From: Tom McNemar Subject: [Histonet] RE: H. Pylori - IHC To: "'Beth.Fye@HCAhealthcare.com'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We get beautiful staining with Dako's concentrate on the Benchmark XT. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth.Fye@HCAhealthcare.com Sent: Wednesday, November 16, 2011 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori - IHC Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 11 Date: Thu, 17 Nov 2011 09:35:34 -0600 From: Glen Dawson Subject: [Histonet] Manual or parts list for a Lipshaw autopsy table To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" All, I was hoping that someone could help me locate an owner's manual or parts list for a Lipshaw Model LM-5-A Autopsy Table. It seems like the hydraulics are going out, but we need the manual to try to address the problem. Thanks In Advance, Glen Dawson BS, HT(ASCP) & QIHC Janesville, WI ------------------------------ Message: 12 Date: Thu, 17 Nov 2011 16:20:53 +0000 From: Sarah Dysart Subject: [Histonet] IHC To: "histonet@lists.utsouthwestern.edu" Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5001FC62@SN2PRD0702MB110.namprd07.prod.ou tlook.com> Content-Type: text/plain; charset="us-ascii" So...I am doing IHC stains with Caspase3 and Ki-67. Does anyone know a positive control for both of these at the same time? Will tonsil pop positive for both?? If not, what are good controls for each of them? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ------------------------------ Message: 13 Date: Thu, 17 Nov 2011 09:23:35 -0700 From: Elizabeth Chlipala Subject: [Histonet] RE: IHC To: 'Sarah Dysart' , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE011380AC70D1@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Tonsil is the control we use Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Thursday, November 17, 2011 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC So...I am doing IHC stains with Caspase3 and Ki-67. Does anyone know a positive control for both of these at the same time? Will tonsil pop positive for both?? If not, what are good controls for each of them? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 17 Nov 2011 16:41:49 +0000 From: Amber McKenzie Subject: RE: [Histonet] RE: Validation To: "Morken, Timothy" , 'Kim Donadio' , "histonet@lists.utsouthwestern.edu" Message-ID: <5A33C952BB67F4468AF1F36D739212BC063249@JERRY.Gia.com> Content-Type: text/plain; charset="us-ascii" So can you do validation backwards? Stain the slides on the new instrument 1st and then stain on the old one to compare instead of looking up old cases to re-run on the new instrument? I have an XT (old) and Ultra (new). From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, November 16, 2011 4:42 PM To: 'Kim Donadio'; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Validation Kim wrote: "Seems the actual amount of validation slides can vary with different Medical directors( Pathologist)" Yes, validation is simply the process of proving to yourself and anyone else (pathologist, clinician, inspector, patient, lawyer) that the product works as intended. Medical Directors are responsible for determining what that entails, whether large or small sets of tissue. There are certain recommendations that have been written up but it all comes down to being able to convince anyone who looks into it that everything is well done. CLIA regulations outline the process and following it will ensure you are on the right track. I have some documents up on a Yahoo groups page that anyone can download. They are from a validation presentation at NSH and ASCP meetings, including the presentation powerpoint outling CLIA and CAP requirements, model validation procedure and model validation documentation forms. You have to join the group but it is free and I promis there will be no emailings to bug you. Go to Yahool groups and search for "Histoinfo" group Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA ________________________________ From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Wednesday, November 16, 2011 12:42 PM To: Morken, Timothy; 'Amber McKenzie'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Validation Seems the actual amount of validation slides can vary with different Medical directors( Pathologist) or thats what Ive seen in places. They( The Pathologist) sign off on it. With that said ER/PR and Her2 have more vigorous criteria( very specific, such as the 25-50). Also, the 2011 AP133 guidline is suppose to go into more detail on this. I havnt seen that new recomendation yet. So If anyone has it, would love to see it posted here myself? Thanks Kim From: "Morken, Timothy" To: 'Amber McKenzie' ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, November 16, 2011 2:08 PM Subject: [Histonet] RE: Validation Amber, is this new instrument the same kind as your old instrument and are you using the same reagents? Or is it a totally different platform with different reagents? Putting in a new instrument of the same kind and using the same reagents just requires verifying the new instrument works. You have to convince whoever wants to know that it works the same as the older instrument so running a variety of antibodies and procedures on it will suffice to prove that. Doing reproducibility tests also proves the instrument works as intended - 5 to 10 identical slides on one run, 5 to 10 identical slides on 5 to 10 runs. If it is a totally different platform with different reagents then you are in for revalidating each of your antibodies on the new system. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, November 16, 2011 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Validation Just wondering what y'alls opinion is on validation: I don't really understand why optimization isn't enough. At that point, the pathologist has said what he wanted the stain to look like, so why do 3-10 positive slides on the new instrument to compare to previous slides from another instrument? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 26 **************************************** ------------------------------ Message: 2 Date: Thu, 17 Nov 2011 14:24:46 -0500 From: "McLaughlin, Terry " Subject: [Histonet] to cut or not to cut mouse brains To: Message-ID: <0B0238D44CE7C04E9D32455C8643D893E3FC0A@wlmmsx02.nemours.org> Content-Type: text/plain; charset="us-ascii" Hello All, I am having trouble cutting frozen mouse brains and was wondering if someone can offer some help. The mouse was perfused in 4% PFA , the brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose for 20 hrs, until the brain sank in this solution. It was frozen back by using a culture plate floating in liquid nitrogen with OCT . The problem I am having is many folds, and air bubbles under the tissue. What I have done so far: Cut at temp of -25, then tried - 22 and -17. Cut at 10um, then tried 16-25um. Tried making the knife colder by adding dry ice to it for a few seconds. Added dry ice to sample for a couple seconds. The sample cuts fine, it appears to happen when picking up on a slide. I tried picking up by starting at one side or end and letting tissue spread onto slide. Did not work. Also tried gently laying slide on top of sample and removing gently-still folds and air bubbles. Any help would be greatly appreciated as we have 6 more to do. Signed, Help! Terry Kokas ------------------------------ Message: 3 Date: Thu, 17 Nov 2011 19:51:57 +0000 From: Helen Fedor Subject: [Histonet] RE: to cut or not to cut mouse brains To: "'McLaughlin, Terry '" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Terry, These are very difficult. The colder the better. (-27). keep the slides in the cryostat. Cut the section. Place section on a cold slide, and keep the slide in the croystat during the following procedure. Hold slide in left hand and brush in right. Place finger under one edge of the section and it will start to thaw, anchoring it to the slide. Gently brush out folds as you continue to advance the thaw line with your fingers under the slide. It takes a bit of practice, and not really perfect. But it works. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McLaughlin, Terry Sent: Thursday, November 17, 2011 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] to cut or not to cut mouse brains Hello All, I am having trouble cutting frozen mouse brains and was wondering if someone can offer some help. The mouse was perfused in 4% PFA , the brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose for 20 hrs, until the brain sank in this solution. It was frozen back by using a culture plate floating in liquid nitrogen with OCT . The problem I am having is many folds, and air bubbles under the tissue. What I have done so far: Cut at temp of -25, then tried - 22 and -17. Cut at 10um, then tried 16-25um. Tried making the knife colder by adding dry ice to it for a few seconds. Added dry ice to sample for a couple seconds. The sample cuts fine, it appears to happen when picking up on a slide. I tried picking up by starting at one side or end and letting tissue spread onto slide. Did not work. Also tried gently laying slide on top of sample and removing gently-still folds and air bubbles. Any help would be greatly appreciated as we have 6 more to do. Signed, Help! Terry Kokas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 17 Nov 2011 11:57:29 -0800 From: "Grantham, Andrea L - (algranth)" Subject: Re: [Histonet] to cut or not to cut mouse brains To: "McLaughlin, Terry" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I'm interested in this answer because this has happened to me - but not always. I usually cut brains at warmer temps. like -17 or -18 as opposed to -25 and they are usually handled in the same manner before being brought to the lab frozen in OCT. Andi On Nov 17, 2011, at 12:24 PM, McLaughlin, Terry wrote: > Hello All, > I am having trouble cutting frozen mouse brains and was wondering if > someone can offer some help. The mouse was perfused in 4% PFA , the > brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose > for 20 hrs, until the brain sank in this solution. It was frozen back by > using a culture plate floating in liquid nitrogen with OCT . > The problem I am having is many folds, and air bubbles under the tissue. > What I have done so far: > Cut at temp of -25, then tried - 22 and -17. > Cut at 10um, then tried 16-25um. > Tried making the knife colder by adding dry ice to it for a few seconds. > Added dry ice to sample for a couple seconds. > The sample cuts fine, it appears to happen when picking up on a slide. I > tried picking up by starting at one side or end and letting tissue > spread onto slide. Did not work. > Also tried gently laying slide on top of sample and removing > gently-still folds and air bubbles. Any help would be greatly > appreciated as we have 6 more to do. > > Signed, > Help! > Terry Kokas > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 5 Date: Thu, 17 Nov 2011 14:58:19 -0500 From: "Connolly, Brett M" Subject: [Histonet] RE: to cut or not to cut mouse brains To: "McLaughlin, Terry " , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Terry, Here what works for us - Slides are kept prechilled in a -20C freezer and then kept cold in the cryostat. Very gently press the cold slide onto the cut section so it makes contact and the section sticks to the slide. Flip slide over and use your finger to warm the back of the slide under the section. Starting with cold slides is a must for us. Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McLaughlin, Terry Sent: Thursday, November 17, 2011 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] to cut or not to cut mouse brains Hello All, I am having trouble cutting frozen mouse brains and was wondering if someone can offer some help. The mouse was perfused in 4% PFA , the brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose for 20 hrs, until the brain sank in this solution. It was frozen back by using a culture plate floating in liquid nitrogen with OCT . The problem I am having is many folds, and air bubbles under the tissue. What I have done so far: Cut at temp of -25, then tried - 22 and -17. Cut at 10um, then tried 16-25um. Tried making the knife colder by adding dry ice to it for a few seconds. Added dry ice to sample for a couple seconds. The sample cuts fine, it appears to happen when picking up on a slide. I tried picking up by starting at one side or end and letting tissue spread onto slide. Did not work. Also tried gently laying slide on top of sample and removing gently-still folds and air bubbles. Any help would be greatly appreciated as we have 6 more to do. Signed, Help! Terry Kokas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 6 Date: Thu, 17 Nov 2011 15:00:37 -0500 From: "Connolly, Brett M" Subject: [Histonet] RE: to cut or not to cut mouse brains To: "McLaughlin, Terry " , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Forgot to mention that we keep the cryostat around -16 to -18 C Brett -----Original Message----- From: Connolly, Brett M Sent: Thursday, November 17, 2011 2:58 PM To: 'McLaughlin, Terry '; histonet@lists.utsouthwestern.edu Subject: RE: to cut or not to cut mouse brains Terry, Here what works for us - Slides are kept prechilled in a -20C freezer and then kept cold in the cryostat. Very gently press the cold slide onto the cut section so it makes contact and the section sticks to the slide. Flip slide over and use your finger to warm the back of the slide under the section. Starting with cold slides is a must for us. Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McLaughlin, Terry Sent: Thursday, November 17, 2011 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] to cut or not to cut mouse brains Hello All, I am having trouble cutting frozen mouse brains and was wondering if someone can offer some help. The mouse was perfused in 4% PFA , the brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose for 20 hrs, until the brain sank in this solution. It was frozen back by using a culture plate floating in liquid nitrogen with OCT . The problem I am having is many folds, and air bubbles under the tissue. What I have done so far: Cut at temp of -25, then tried - 22 and -17. Cut at 10um, then tried 16-25um. Tried making the knife colder by adding dry ice to it for a few seconds. Added dry ice to sample for a couple seconds. The sample cuts fine, it appears to happen when picking up on a slide. I tried picking up by starting at one side or end and letting tissue spread onto slide. Did not work. Also tried gently laying slide on top of sample and removing gently-still folds and air bubbles. Any help would be greatly appreciated as we have 6 more to do. Signed, Help! Terry Kokas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 7 Date: Thu, 17 Nov 2011 20:13:05 +0000 From: Helen Fedor Subject: RE: [Histonet] to cut or not to cut mouse brains To: "'Grantham, Andrea L - (algranth)'" , "McLaughlin, Terry" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" When the blocks are not croyprotected you can cut them at warmer temperatures. But when they are cryoprotected with the 30% sucrose, they are soft at -18, that is why you need the colder temperature. they will compress at the warmer temperatures and give you a lot of wrinkles. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Thursday, November 17, 2011 2:57 PM To: McLaughlin, Terry Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] to cut or not to cut mouse brains I'm interested in this answer because this has happened to me - but not always. I usually cut brains at warmer temps. like -17 or -18 as opposed to -25 and they are usually handled in the same manner before being brought to the lab frozen in OCT. Andi On Nov 17, 2011, at 12:24 PM, McLaughlin, Terry wrote: > Hello All, > I am having trouble cutting frozen mouse brains and was wondering if > someone can offer some help. The mouse was perfused in 4% PFA , the > brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose > for 20 hrs, until the brain sank in this solution. It was frozen back > by using a culture plate floating in liquid nitrogen with OCT . > The problem I am having is many folds, and air bubbles under the tissue. > What I have done so far: > Cut at temp of -25, then tried - 22 and -17. > Cut at 10um, then tried 16-25um. > Tried making the knife colder by adding dry ice to it for a few seconds. > Added dry ice to sample for a couple seconds. > The sample cuts fine, it appears to happen when picking up on a slide. > I tried picking up by starting at one side or end and letting tissue > spread onto slide. Did not work. > Also tried gently laying slide on top of sample and removing > gently-still folds and air bubbles. Any help would be greatly > appreciated as we have 6 more to do. > > Signed, > Help! > Terry Kokas > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Thu, 17 Nov 2011 14:34:55 -0600 From: "Morocho, Jennifer" Subject: [Histonet] HTL Lead Job Opening To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" The University of MN Medical Center, Fairview has an exciting and immediate job opportunity as an HTL Technical Lead in Minneapolis, MN. Check out the Fairview web site www.fairview.org and look for job 11-36731. Or contact me, Jennifer Morocho, HTL (ASCP)CM at jmoroch1@fairview.org for more details. The Pathology Supervisor and I who would love to talk opportunity with you! ------------------------------ Message: 9 Date: Thu, 17 Nov 2011 15:37:03 -0500 From: "Goodwin, Diana" Subject: [Histonet] Brass cryostat chucks To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <09411E0112A96A459D8D5FBDAB9C15C72480DAE2DF@HAMEXMBA.rwjham.local> Content-Type: text/plain; charset="us-ascii" Greeting, Histonetters. I am in search of those brass cryostat chucks with the holes in them. Any ideas? Googled my brains out with no luck. Diana G. Goodwin, BS, HT(ASCP)QIHC Department of Pathology Robert Wood Johnson University Hospital at Hamilton One Hamilton Health Place Hamilton, NJ 08690 Ph: 609.631.6996 Email: dgoodwin@rwjuhh.edu ------------------------------ Message: 10 Date: Thu, 17 Nov 2011 16:33:49 -0500 From: "Ladd, Sharron" Subject: [Histonet] Source of IgG4 for Isotype control To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <1EC9E4133CB4EB4A90383226A986461404CB34A93E@wal-mail02> Content-Type: text/plain; charset="us-ascii" Hi Histonet, Where can I buy IgG4 in the US please? Thanks, Sharron ------------------------------ Message: 11 Date: Thu, 17 Nov 2011 13:39:26 -0800 From: "Grantham, Andrea L - (algranth)" Subject: Re: [Histonet] Brass cryostat chucks To: "Goodwin, Diana" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" For what cryostat? On Nov 17, 2011, at 1:37 PM, Goodwin, Diana wrote: > Greeting, Histonetters. > > I am in search of those brass cryostat chucks with the holes in them. Any ideas? Googled my brains out with no luck. > > > Diana G. Goodwin, BS, HT(ASCP)QIHC > > Department of Pathology > > Robert Wood Johnson University Hospital at Hamilton > > One Hamilton Health Place > > Hamilton, NJ 08690 > > Ph: 609.631.6996 > > Email: dgoodwin@rwjuhh.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 12 Date: Thu, 17 Nov 2011 15:50:13 -0600 From: Christina.Wilson@leica-microsystems.com Subject: [Histonet] AUTO: is out of the office. (returning Wed 10/19/2011) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I am out of the office from Thu 11/17/2011 until Fri 11/18/2011. I will have limited access to emails during this time. If you should need assistance, please contact Demaris Mills, demaris.mills@leica-microsystems.com, for product management support or Karen Niewerth, karen.niewerth@leica-microsystems.com, for customer service support. Note: This is an automated response to your message "Histonet Digest, Vol 96, Issue 26" sent on 11/17/2011 12:02:06 PM. This is the only notification you will receive while this person is away. ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ ------------------------------ Message: 13 Date: Thu, 17 Nov 2011 16:02:50 -0600 From: Salomao Segal Subject: [Histonet] microtomes To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 The settings in a rotary microtome may indicate the thickness of sections but... how do you know that it indeed cuts at the indicated thickness, particularly if it is say an old device that you inherit from somebody else's lab junk? Is there a way of measuring the magnitude of advances after each rotation? Thanks Solomon Segal ------------------------------ Message: 14 Date: Thu, 17 Nov 2011 15:09:21 -0800 (PST) From: Cheryl Subject: [Histonet] broken chuck holder To: "histonet@lists.utsouthwestern.edu" Message-ID: <1321571361.65679.YahooMailNeo@web39409.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Is your tech using anything like ammonia or soap on her ice to soak the blocks? This would chew through the spring much more quickly than just ice water...??? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax admin@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. ------------------------------ Message: 15 Date: Thu, 17 Nov 2011 15:46:16 -0800 From: connie grubaugh Subject: RE: [Histonet] Paraplast X-tra To: , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" We are having problems again too. Have not changed a thing and convinced that it is a bad lot again. Wonder sometimes on the QC that is done on the paraffin. Connie G. > Date: Wed, 16 Nov 2011 13:47:28 -0800 > From: one_angel_secret@yahoo.com > To: cls71877@sbcglobal.net; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Paraplast X-tra > CC: > > Maybe you got a bad lot that is contaminated causing the knife marks? Also, Ive noticed that if you let your paraffin get to hot, it breaks down and that could be one reason for a consistency issue. Have you changed anything at all recently to your process? Hope this was helpful. > > Kim > > > ________________________________ > From: Cristi stephenson > To: Histo Net > Sent: Wednesday, November 16, 2011 2:06 PM > Subject: [Histonet] Paraplast X-tra > > Hello Histoland, > We are seeing unusually large volumes of fall out in our paraffin lately. > The precipitate is almost "muddy" or "oily" in appearance. We noticed it > immediately as it was melting, so there was no way anything else had been > introduced to cause this. As a result, we are seeing knife marks. The marks > are not consistent and not attributable to any of the tissue in the blocks. We > are blasting through blades that have been tried and true for years now. Are > there any other users of paraplast X-tra experiencing these issues? > > Thanks, > Cristi > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Thu, 17 Nov 2011 16:08:33 -0800 From: " cls71877@sbcglobal.net " Subject: Re: [Histonet] Paraplast X-tra To: " connie grubaugh " , one_angel_secret@yahoo.com, histonet@lists.utsouthwestern.edu Message-ID: <74764.79442.bm@smtp214.mail.bf1.yahoo.com> Content-Type: text/plain; charset=utf-8 I had to believe someone else out there was seeing this too!! Our rep contacted us today and is going to replace it and investigate...I too wonder how this gets past the QC dept., it is super obvious as soon as it starts melting.... Sent from my HTC on the Now Network from Sprint! ----- Reply message ----- From: "connie grubaugh" Date: Thu, Nov 17, 2011 3:46 pm Subject: [Histonet] Paraplast X-tra To: , , ------------------------------ Message: 17 Date: Thu, 17 Nov 2011 20:32:04 -0700 From: "Karen Lahti" Subject: RE: [Histonet] RE: H. Pylori - IHC To: "'Tom McNemar'" , , Message-ID: <007701cca5a2$a4e88a90$eeb99fb0$@com> Content-Type: text/plain; charset="us-ascii" We use Biocare antibody with great results on the IntelliPath from Biocare and the Bondmax from Leica. Karen Lahti Arizona Digestive Health -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, November 17, 2011 6:30 AM To: 'Beth.Fye@HCAhealthcare.com'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: H. Pylori - IHC We get beautiful staining with Dako's concentrate on the Benchmark XT. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth.Fye@HCAhealthcare.com Sent: Wednesday, November 16, 2011 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori - IHC Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 96, Issue 27 **************************************** From catialopes43 <@t> gmail.com Fri Nov 18 11:04:57 2011 From: catialopes43 <@t> gmail.com (Catia Lopes) Date: Fri Nov 18 11:05:01 2011 Subject: [Histonet] Problems in processing rat paws Message-ID: Hello everyone! I have a problem in processing rat paws to paraffin embedding. I need to process and cut the whole paw (except fingers). So, first I need to decalcify the paw and then process it to paraffin embedding. My problem is that when I try to cut the paw I can not get a good section because the tissue have a gum-like consistence (mushy). Do you know where is my problem? Dehydration, clearing, embedding? ** *Note*: I decalcify the paws in formic acid and aluminum chloride for 24h; Then I wash samples in phosphate buffer saline for 5 hours. After that I did the following manual processing (applying vacuum): 2x30min in 70% ethanol, 2x30min in 80% ethanol, 2x30min in 96% ethanol, 2x30min in absolute ethanol, 3x15 min in Xilene, 2x15min in paraffin and a final incubation of 30min in paraffin. Then, I proceed to paraffin ambedding and try to cut sections of 5um of thickness. If anyone can help me I will be grateful. Thanks in advance, C?tia From liz <@t> premierlab.com Fri Nov 18 11:15:40 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Nov 18 11:15:49 2011 Subject: [Histonet] Problems in processing rat paws In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE011380AC7102@SBS2K8.premierlab.local> Catia Its going to take about a week to decal those types of samples in formic acid and in addition to that the processing cycle needs to be considerably longer. By rat paw, I'm assuming you are mean the front paw or is it the rear ankle that you need to process. First of all the samples need to be fixed for at least 48 hours prior to decalcification. We use 10% formic acid in distilled water, seems to work fine for us. For ankle samples without the rear toes, it takes about a week to decal. If the toes are attached in our hands it takes about two weeks, we change the decal about every third day or so and always on the day prior to processing. Ankles are trimmed in half and processed, both halves in one cassette. For ankle and toes, we trim one side of the joint off prior to processing. For front paws since they are a bit thick I would trim off some of the wrist pad prior to processing. Processing cycle is long. 1 hour in each station for ankles and 1 to 2 hours per station for ankles with toes. We use three absolutes and three xylenes and 4 paraffins when processing. 70% - 1 hour 80% - 1 hour 95% - 1 hour 100% - 1 hour 100% - 1 hour 100% - 1 hour Xylene - 1 hour Xylene - 1 hour Xylene - 1 hour Paraffin - 1 hour Paraffin - 1 hour Paraffin - 1 hour Good luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catia Lopes Sent: Friday, November 18, 2011 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problems in processing rat paws Hello everyone! I have a problem in processing rat paws to paraffin embedding. I need to process and cut the whole paw (except fingers). So, first I need to decalcify the paw and then process it to paraffin embedding. My problem is that when I try to cut the paw I can not get a good section because the tissue have a gum-like consistence (mushy). Do you know where is my problem? Dehydration, clearing, embedding? ** *Note*: I decalcify the paws in formic acid and aluminum chloride for 24h; Then I wash samples in phosphate buffer saline for 5 hours. After that I did the following manual processing (applying vacuum): 2x30min in 70% ethanol, 2x30min in 80% ethanol, 2x30min in 96% ethanol, 2x30min in absolute ethanol, 3x15 min in Xilene, 2x15min in paraffin and a final incubation of 30min in paraffin. Then, I proceed to paraffin ambedding and try to cut sections of 5um of thickness. If anyone can help me I will be grateful. Thanks in advance, C?tia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From agetliang <@t> gmail.com Fri Nov 18 11:17:36 2011 From: agetliang <@t> gmail.com (aget liang) Date: Fri Nov 18 11:17:39 2011 Subject: [Histonet] clearing of whole tissue Message-ID: Hi, anyone has experience in tissue clearing? i want to clear spinal cord after fixation. Thanks. YJ -- Truth Shall Make You Free From relia1 <@t> earthlink.net Fri Nov 18 11:54:09 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Nov 18 11:54:11 2011 Subject: [Histonet] RELIA Hot Histology Job Alert - Histology Tech needed in Virginia. Can you help? Message-ID: <10AA804B13E344B1928FBE101C007FB0@ownerf1abaad51> Hi Histonetters!! I hope you are gearing up for a fun Pre-Thanksgiving weekend. I have a great job opportunity to tell you about. I am currently working with one of my best clients and they are in need of a histotech. I refer to them as one of my best clients because they have very low turnover and I have only placed people with them due to growth. This position is for their lab in Roanoke, VA. This is a full time day shift M-F position. My client offers a great compensation package and an awesome team to work with. If you are ASCP HT/HTL or eligible, have at least 2-3 years of routine histology experience (IHC is a plus) and would like to live in beautiful Roanoke, VA then my client wants to talk to you. Whether you would like to start a new position RIGHT AWAY or would prefer to start a new position AFTER the holidays the choice is up to you. My client is very flexible. If you would like more information please contact me. I can be reached toll free at 866-607-3542 or via email at relia1@earthlink.net. If you know someone else that might be interested please feel free to pass my information along. If you refer someone to me and I place them you will receive a referral bonus. (That sure could come in handy with the holidays around the corner) :). Thanks for taking the time to read this post and Enjoy Your Weekend. >Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From leiker <@t> buffalo.edu Fri Nov 18 11:59:34 2011 From: leiker <@t> buffalo.edu (Leiker, Merced) Date: Fri Nov 18 11:59:43 2011 Subject: [Histonet] microtomes In-Reply-To: References: Message-ID: Do you have access to a confocal microscope or one that does Z-stack imaging? You can find out the thickness of your tissue sections that way (after you've mounted them on a slide of course). Regards, Merced -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, November 18, 2011 8:55 AM To: histonet@lists.utsouthwestern.edu; ssegal2@slu.edu Subject: [Histonet] microtomes Hi, If you are concerned about the thickness of the sections being accurate to the setting, I would suggest picking up a cheap micrometer from a hardware store. (OK perhaps not cheap as you will want a quality one, but the cost of these isn't terrible.) You can't really measure 5 microns (or whatever you cut at) well, so you will need to take 10-50 sections of a blank block. Remember to keep a constant rhythm that you would use on a normal block. Measure the thickness before and after then divide these thicknesses by the number of sections taken. Remember, also, to take the measurements at the same temperature that you are cutting the sections at. If your difference isn't exactly right how far off is it? You would then know if you should use a higher or lower setting on the microtome. Amos On Thu, Nov 17, 2011 at 10:33 PM, wrote: > Message: 13 > Date: Thu, 17 Nov 2011 16:02:50 -0600 > From: Salomao Segal > Subject: [Histonet] microtomes > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > > Content-Type: text/plain; charset=ISO-8859-1 > > The settings in a rotary microtome may indicate the thickness of > sections but... > > how do you know that it indeed cuts at the indicated thickness, > particularly if it is say an old device that you inherit from somebody > else's lab junk? > > Is there a way of measuring the magnitude of advances after each rotation? > > Thanks > > > Solomon Segal > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From making <@t> ufl.edu Fri Nov 18 12:20:34 2011 From: making <@t> ufl.edu (MKing) Date: Fri Nov 18 12:20:35 2011 Subject: [Histonet] clearing spinal cord Message-ID: <4EC6A1F2.4040903@ufl.edu> YJ, You would probably be interested in this: Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain. Hiroshi Hama et al., Nature Neuroscience 14, 1481?1488 (2011) --------------- Message: 1 Date: Fri, 18 Nov 2011 09:17:36 -0800 From: aget liang Subject: [Histonet] clearing of whole tissue To: histonet@lists.utsouthwestern.edu Message-ID: Hi, anyone has experience in tissue clearing? i want to clear spinal cord after fixation. Thanks. YJ From d-emge <@t> northwestern.edu Fri Nov 18 12:34:25 2011 From: d-emge <@t> northwestern.edu (Donna J Emge) Date: Fri Nov 18 12:35:26 2011 Subject: [Histonet] Re: To cut or not to cut mouse brains Message-ID: <54C3042A8564BA4AA2D355DD1D4C949CFBF313@chcspmbx2.ads.northwestern.edu> I have had folding and bubbles occur periodically with PFA fixed, sucrose cryoprotected frozen brain tissue I receive from other labs. I streak a distilled water moistened brush across the slide then pick up the frozen section on the moistened area. It comes out flat and smooth every time. I let the section dry as usual, then store in -20?C or -80?C. For ISH I use a RNase zapped and DEPC rinsed brush and streak DEPC water across the slide with the moistened brush. I section the PFA fixed, sucrose cryoprotected tissue around -21?C. Researchers report good subsequent IHC and ISH results and bring more samples. Donna J. Emge, ASCP-HT Mouse Histology and Phenotyping Laboratory Manager Northwestern University Olson Pavilion 8-333 710 North Fairbanks Court Chicago, IL 60611 d-emge@northwestern.edu 312-503-2679 From mhale <@t> carisls.com Fri Nov 18 12:56:13 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Fri Nov 18 12:56:20 2011 Subject: [Histonet] PT Testing Message-ID: <6F33D8418806044682A391273399860F0AA1E33B@s-irv-ex301.PathologyPartners.intranet> How are those of you in Technical Component labs handling your PT testing ? And if you are grading these slides how are you setting the criteria and what are you looking at ? Thanks Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From TNMayer <@t> mdanderson.org Fri Nov 18 13:35:21 2011 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Fri Nov 18 13:35:24 2011 Subject: [Histonet] Paraplast X-tra In-Reply-To: <6c733c08-a969-4b88-bb4f-fff21ca92232@DCPWPRTR04.mdanderson.edu> References: <6c733c08-a969-4b88-bb4f-fff21ca92232@DCPWPRTR04.mdanderson.edu> Message-ID: Hey Connie, Remember that we experienced a drastic heat wave all over the country and in some parts there were rolling blackouts. The product may have been stored in a warehouse that experienced a rolling blackout and the increased temperature messed up the paraffin which then re-hardened and was sold.The bag looked fine, so nobody thought anything was wrong. Stranger things have happened. The overheating would cause the oily and muddy. I say this because a few years back we had a problem with coverslips that were stuck together. Even though they were new and still sealed, when opened they were stuck. It turned out there was excess moisture in the warehouse and the silica gel didn't work. We returned them and got replacements. Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Message: 15 Date: Thu, 17 Nov 2011 15:46:16 -0800 From: connie grubaugh Subject: RE: [Histonet] Paraplast X-tra To: , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" We are having problems again too. Have not changed a thing and convinced that it is a bad lot again. Wonder sometimes on the QC that is done on the paraffin. Connie G. > Date: Wed, 16 Nov 2011 13:47:28 -0800 > From: one_angel_secret@yahoo.com > To: cls71877@sbcglobal.net; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Paraplast X-tra > CC: > > Maybe you got a bad lot that is contaminated causing the knife marks? Also, Ive noticed that if you let your paraffin get to hot, it breaks down and that could be one reason for a consistency issue. Have you changed anything at all recently to your process? Hope this was helpful. > > Kim > > > ________________________________ > From: Cristi stephenson > To: Histo Net > Sent: Wednesday, November 16, 2011 2:06 PM > Subject: [Histonet] Paraplast X-tra > > Hello Histoland, > We are seeing unusually large volumes of fall out in our paraffin lately. > The precipitate is almost "muddy" or "oily" in appearance. We noticed it > immediately as it was melting, so there was no way anything else had been > introduced to cause this. As a result, we are seeing knife marks. The marks > are not consistent and not attributable to any of the tissue in the blocks. We > are blasting through blades that have been tried and true for years now. Are > there any other users of paraplast X-tra experiencing these issues? > > Thanks, > Cristi > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Thu, 17 Nov 2011 16:08:33 -0800 From: " cls71877@sbcglobal.net " Subject: Re: [Histonet] Paraplast X-tra To: " connie grubaugh " , one_angel_secret@yahoo.com, histonet@lists.utsouthwestern.edu Message-ID: <74764.79442.bm@smtp214.mail.bf1.yahoo.com> Content-Type: text/plain; charset=utf-8 I had to believe someone else out there was seeing this too!! Our rep contacted us today and is going to replace it and investigate...I too wonder how this gets past the QC dept., it is super obvious as soon as it starts melting.... Sent from my HTC on the Now Network from Sprint! ----- Reply message ----- From: "connie grubaugh" Date: Thu, Nov 17, 2011 3:46 pm Subject: [Histonet] Paraplast X-tra To: , , ------------------------------ ******** From carl.hobbs <@t> kcl.ac.uk Fri Nov 18 14:33:33 2011 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri Nov 18 14:33:39 2011 Subject: [Histonet] re: clearing of whole tissue Message-ID: <11D9615B89C10747B1C985966A63D7CA38276092FA@KCL-MAIL04.kclad.ds.kcl.ac.uk> Well, I have used all of the "usual suspects" over many years but, this one has taken me by complete surprise! If anyone can explain the rationale of the reaction, I would be most interested. One for J Kiernan, I think? Ah, yes: "Sca/e: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain" Fascinating! I love the forward slash before the e....how should it be pronounced? Is it related to Wall-e???? ( Many thanks for the info, Squash buddy Phil ;-) Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 From mpowers <@t> dpspa.com Fri Nov 18 15:43:40 2011 From: mpowers <@t> dpspa.com (Marian Powers) Date: Fri Nov 18 15:43:38 2011 Subject: [Histonet] Fwd: IHC control References: Message-ID: -Marian Begin forwarded message: > From: Marian Powers > Date: November 16, 2011 12:51:56 PM EST > To: histonet@lists.utsouthwestern.edu > Subject: IHC control > > Hi: Would anyone have a hereditary nonpolyposis colorectal cancer control, (for MHL1, MSH2, MSH6) to share? > > Thanks in advance, > > -- > > Marian L. Powers > > Doctors Pathology Services > c| 302.747.0580 > o| 302.677.0000 ext: 110 > f | 302.677.0010 > > > > > > > > > From agetliang <@t> gmail.com Fri Nov 18 18:40:09 2011 From: agetliang <@t> gmail.com (aget liang) Date: Fri Nov 18 18:40:12 2011 Subject: [Histonet] Re: clearing of whole tissue In-Reply-To: References: Message-ID: Did anyone know what is the buffer system for the 4M urea? it is important to adjust and maintain pH to 7.7 according to the paper: Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain 2011/11/18 aget liang > Hi, anyone has experience in tissue clearing? i want to clear spinal cord > after fixation. Thanks. YJ > > -- > Truth Shall Make You Free > -- Truth Shall Make You Free From jkiernan <@t> uwo.ca Fri Nov 18 23:58:44 2011 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Nov 18 23:58:48 2011 Subject: [Histonet] Re: clearing of whole tissue In-Reply-To: <74e087c482754.4ec7458a@uwo.ca> References: <74e0bbd7852b8.4ec741c2@uwo.ca> <75e0a9f3831f6.4ec741ff@uwo.ca> <7640f46a81be5.4ec7423c@uwo.ca> <74f0f44f87427.4ec7427c@uwo.ca> <74e087c482754.4ec7458a@uwo.ca> Message-ID: <7570a89784793.4ec6ff44@uwo.ca> The solution described in the paper does not mention a buffer. Hama et al (2011) wrote: "The most effective solution,which we named ScaleA2, was composed of 4 M urea, 10% (wt/vol) glycerol and 0.1% (wt/vol) Triton X-100." In the next sentence they said the pH was 7.7. Later in the paper they describe a modified solution suitable for embryos, which has more glycerol: "Thus, we developed a modified solution, ScaleU2, composed of 4 M urea, 30% glycerol and 0.1% Triton X-100. ScaleU2 requires longer incubation times to achieve clearing, but it reduces tissue expansion and the fragility of cleared brain samples and is therefore suitable for soft samples such as mouse embryos." No mention of pH here. The paper contains spectacular images. John Kiernan Anatomy, UWO London, Canada = = = On 18/11/11, aget liang wrote: > > Did anyone know what is the buffer system for the 4M urea? it is important > to adjust and maintain pH to 7.7 according to the paper: > Scale: a chemical approach for fluorescence imaging and reconstruction of > transparent mouse brain > > 2011/11/18 aget liang > > > Hi, anyone has experience in tissue clearing? i want to clear spinal cord > > after fixation. Thanks. YJ > > > > -- > > Truth Shall Make You Free > > > > > > -- > Truth Shall Make You Free > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From agetliang <@t> gmail.com Sat Nov 19 06:57:31 2011 From: agetliang <@t> gmail.com (aget liang) Date: Sat Nov 19 06:57:35 2011 Subject: [Histonet] Re: clearing of whole tissue In-Reply-To: <7570a89784793.4ec6ff44@uwo.ca> References: <74e0bbd7852b8.4ec741c2@uwo.ca> <75e0a9f3831f6.4ec741ff@uwo.ca> <7640f46a81be5.4ec7423c@uwo.ca> <74f0f44f87427.4ec7427c@uwo.ca> <74e087c482754.4ec7458a@uwo.ca> <7570a89784793.4ec6ff44@uwo.ca> Message-ID: thanks, i contact the author, according to Dr.Atsushi Miyawaki, there should be no salt in the scaLe solution for clearing of tissues. so the urea is dissolved in miliQ pure water and adjustment of pH is not necessary, i guess, for urea. 2011/11/19 John Kiernan > The solution described in the paper does not mention a buffer. Hama et al > (2011) wrote: "The most effective solution,which we named ScaleA2, was > composed of 4 M urea, 10% (wt/vol) glycerol and 0.1% (wt/vol) Triton > X-100." In the next sentence they said the pH was 7.7. Later in the paper > they describe a modified solution suitable for embryos, which has more > glycerol: "Thus, we developed a modified solution, ScaleU2, composed of 4 M > urea, 30% glycerol and 0.1% Triton X-100. ScaleU2 requires longer > incubation times to achieve clearing, but it reduces tissue expansion and > the fragility of cleared brain samples and is therefore suitable for soft > samples such as mouse embryos." No mention of pH here. > > The paper contains spectacular images. > > John Kiernan > Anatomy, UWO > London, Canada > = = = > On 18/11/11, *aget liang * wrote: > > Did anyone know what is the buffer system for the 4M urea? it is important > to adjust and maintain pH to 7.7 according to the paper: > Scale: a chemical approach for fluorescence imaging and reconstruction of > transparent mouse brain > > 2011/11/18 aget liang > > > Hi, anyone has experience in tissue clearing? i want to clear spinal cord > > after fixation. Thanks. YJ > > > > -- > > Truth Shall Make You Free > > > > > > -- > Truth Shall Make You Free > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- Truth Shall Make You Free From shrivastavaanuradha <@t> hotmail.com Sat Nov 19 11:40:25 2011 From: shrivastavaanuradha <@t> hotmail.com (anuradha shrivastava) Date: Sat Nov 19 11:40:30 2011 Subject: [Histonet] PARAPLAST XTRA Message-ID: Hello , I have seen the same thing in my paraffin dispenser, oily, muddy sedimentation. The paraffin pallets in the packet look like partially melted. May be some issues with the storage . Thanks for the info. every one. Anu. From adesupo2002 <@t> hotmail.com Sat Nov 19 23:34:44 2011 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Sat Nov 19 23:34:48 2011 Subject: [Histonet] Handling Muscle, Nerves and Renal biopsy specimens Message-ID: Hi, Please I am wondering if you guys could help me by telling me how you handle specimens like Muscle, Nerve and Renal biopsy after the working hours like may be at night or weekend. Thank you all for your usual cooperation. Ade. From epeters2 <@t> gmu.edu Sun Nov 20 18:44:14 2011 From: epeters2 <@t> gmu.edu (Esther C Peters) Date: Sun Nov 20 18:44:17 2011 Subject: [Histonet] Senior Research Assistant Position in Histology Message-ID: Please see the following job announcement for a Senior Research Assistant at George Washington University in Washington, DC. Contact Dr. Patricia Latham at GWU if you are interested in this position: platham@mfa.gwu.edu Full-time Research Assistant - Histotechnologist A position is available for a histotechnologist in a new research core laboratory in the GWU School of Biomedical Sciences. This person will be expected to maintain a histology laboratory, including ordering of supplies, washing glassware and maintaining equipment for optimal function, daily logs with inventory of specimens and blocks, labeling of samples and slides, tissue processing and embedding, microtome and cryostat sections, routine stains, special stains and immunostains with appropriate controls, as required to meet the needs of researchers at GWU. This person will need to work independently to deliver excellent quality results in a timely manner. The person should have strong communication skills and managerial skills, since there will be a need to work with researchers in several labs and to maintain inventory from several sources, to keep track of the flow of specimens and charges. The position will require data entry into an audit-trail database and the mainten ance of meticulous records, with an exchange of data to the research center off-site and to labs at international sites. This person should have some experience in laboratory research, implementation of new techniques and the ability to trouble-shoot problems that might arise in this setting. Ideally, the person will have an entreprenurial spirit, since the success of the lab will determine its future existence. The full-time position is assured for 2 years, but it can be extended and/ or grow to full-time, if the lab is successful. Supervision: The work will be supervised by a Pathologist as Project Manager. Entry level qualifications: ? Bachelor?s degree in a clinical science with a course in Histology or equivalent combination of training and experience. ? Experience working in a research setting. ? Favored is eligibility or certification as a Histotechnologist or Histotechnician by the American Society for Clinical Pathology. Esther C. Peters, Ph.D. Assistant Professor Department of Environmental Science & Policy Biology Program/Medical Technology Coordinator George Mason University 4400 University Drive, MSN 5F2 Fairfax, VA 22030-4444 Office: David King Hall 3057 Phone: 703-993-3462 Fax: 703-993-1066 epeters2@gmu.edu From Nancy_Schmitt <@t> pa-ucl.com Mon Nov 21 08:54:38 2011 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Mon Nov 21 08:54:48 2011 Subject: [Histonet] Handling Muscle, Nerves and Renal biopsy specimens In-Reply-To: <20111120180230.2DAD11BEEA9@mail.pa-ucl.com> References: <20111120180230.2DAD11BEEA9@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B97FB@PEITHA.wad.pa-ucl.com> These cases are only scheduled during regular hours M-F. Nancy ----------------------------------------------------- Message: 1 Date: Sun, 20 Nov 2011 00:34:44 -0500 From: ADESUPO ADESUYI Subject: [Histonet] Handling Muscle, Nerves and Renal biopsy specimens To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, Please I am wondering if you guys could help me by telling me how you handle specimens like Muscle, Nerve and Renal biopsy after the working hours like may be at night or weekend. Thank you all for your usual cooperation. Ade. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From joseph-galbraith <@t> uiowa.edu Mon Nov 21 09:14:58 2011 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Mon Nov 21 09:15:05 2011 Subject: [Histonet] RE: Handling Muscle, Nerves and Renal biopsy specimens In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B97FB@PEITHA.wad.pa-ucl.com> References: <20111120180230.2DAD11BEEA9@mail.pa-ucl.com> <906B4DA90ED1DB4DB6C7E94D7CEE6C367B97FB@PEITHA.wad.pa-ucl.com> Message-ID: <6DC87DEA9229894DB3A09F8B61717A4610724977@hc-mailboxc1-n3.healthcare.uiowa.edu> Ade: I would agree with Nancy on muscle and nerve biopsies but not for renal biopsies. We have a tech on call to handle 'emergent' renal/liver biopsies on the weekends and holidays. These do happen rather frequently outside of regular business hours at my institution. How you choose to handle these will depend on the frequency and urgency of the situation at your institution. You can send me a separate email if you want more details. Joe joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Monday, November 21, 2011 8:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Handling Muscle, Nerves and Renal biopsy specimens These cases are only scheduled during regular hours M-F. Nancy ----------------------------------------------------- Message: 1 Date: Sun, 20 Nov 2011 00:34:44 -0500 From: ADESUPO ADESUYI Subject: [Histonet] Handling Muscle, Nerves and Renal biopsy specimens To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, Please I am wondering if you guys could help me by telling me how you handle specimens like Muscle, Nerve and Renal biopsy after the working hours like may be at night or weekend. Thank you all for your usual cooperation. Ade. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From pathlocums <@t> gmail.com Mon Nov 21 11:41:22 2011 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Mon Nov 21 11:41:26 2011 Subject: [Histonet] Chemical Recycling - B/R Instruments Message-ID: Can anyone provide me information on B/R Instruments Pro series recyclers? I am considerning them vs. CBG Biotech. I have heard rumors that the B/R units throw a lot of heat from the lower boiler and damage the underlying flooring. Can anyone confirm this, or any other troubles with their units? Thank you. -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From pathlocums <@t> gmail.com Mon Nov 21 11:44:18 2011 From: pathlocums <@t> gmail.com (Davide Costanzo) Date: Mon Nov 21 11:44:26 2011 Subject: [Histonet] Per Diem work in LA - Beverly Hills area Message-ID: Hello all - We are opening a histology laboratory in the Beverly Hills area, and are in need of per diem histotech's to cover vacation/sickness or upticks in volume. If anyone in the LA area is interested in doing occassional work for a few hours at a time to supplement their current job please contact me, not by reply here but at my office email : david@blufrogpath.com Thank you. Happy Thanksgiving to all. -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 From rjbuesa <@t> yahoo.com Mon Nov 21 11:47:11 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 21 11:47:18 2011 Subject: [Histonet] Chemical Recycling - B/R Instruments In-Reply-To: Message-ID: <1321897631.31877.YahooMailClassic@web65707.mail.ac4.yahoo.com> My B/R xylene/alcohol recycling unit was placed over a counter and did not cause any damage to it?after several years of continuous and reliable usage. It?paid itself in 3 years from savings of xylene that I did not have to buy. I do not know about the other brand you are asking about, but I do not?think it will better than B/R Ren? J.? --- On Mon, 11/21/11, Davide Costanzo wrote: From: Davide Costanzo Subject: [Histonet] Chemical Recycling - B/R Instruments To: histonet@lists.utsouthwestern.edu Date: Monday, November 21, 2011, 12:41 PM Can anyone provide me information on B/R Instruments Pro series recyclers? I am considerning them vs. CBG Biotech. I have heard rumors that the B/R units throw a lot of heat from the lower boiler and damage the underlying flooring. Can anyone confirm this, or any other troubles with their units? Thank you. -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Mon Nov 21 12:21:52 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Nov 21 12:22:02 2011 Subject: [Histonet] Chemical Recycling - B/R Instruments In-Reply-To: References: Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323E5E7DFFE@LRGHEXVS1.practice.lrgh.org> Have never had a problem with ours. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo Sent: Monday, November 21, 2011 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chemical Recycling - B/R Instruments Can anyone provide me information on B/R Instruments Pro series recyclers? I am considerning them vs. CBG Biotech. I have heard rumors that the B/R units throw a lot of heat from the lower boiler and damage the underlying flooring. Can anyone confirm this, or any other troubles with their units? Thank you. -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From pkrichar <@t> gundluth.org Mon Nov 21 12:25:20 2011 From: pkrichar <@t> gundluth.org (Richardson, Pam K) Date: Mon Nov 21 12:28:29 2011 Subject: [Histonet] Laboratory Call Center Message-ID: <998284C32F61104CA0BEFFFFCF6F90FD42475A@LXEXMB03.gundluth.org> I am looking to talk with someone whose lab has a call center. Cordially, Pam ~ ++++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Lutheran Laboratory Email: pkrichar@gundluth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundluth.org From hrfulklab <@t> gmail.com Mon Nov 21 12:45:37 2011 From: hrfulklab <@t> gmail.com (H R) Date: Mon Nov 21 12:45:40 2011 Subject: [Histonet] Signs Message-ID: Does anyone know the CLIA requirements for what sort of sign that is required on the laboratory door? thanks From gu.lang <@t> gmx.at Mon Nov 21 12:49:32 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Nov 21 12:49:46 2011 Subject: [Histonet] recycling solvents Message-ID: <84B344439E2C4E838577648AF1D47D03@dielangs.at> Hi all! What is the general opinion about recycling ethanol and xylen? Experiences? Reasonable volumes? I would be glad about any comment. Regards Gudrun Lang From madeleinehuey <@t> gmail.com Mon Nov 21 12:52:14 2011 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Mon Nov 21 12:52:24 2011 Subject: [Histonet] Re: Histonet Digest, Vol 96, Issue 32 In-Reply-To: <4eca91e8.4aafec0a.3880.ffffbacdSMTPIN_ADDED@mx.google.com> References: <4eca91e8.4aafec0a.3880.ffffbacdSMTPIN_ADDED@mx.google.com> Message-ID: I have an immediately "Per Diem" Histotech position available in Mountain View, CA. The hours are somewhat flexible, with 4 hr - 5/day (Monday to Friday). Candidate must be able to embed approx. 50 blocks/hr and cut 100 - 200 blocks/day. Madeleine Supervisor-Pathology El Camino Hospital 2500 Grant Road Mountain View, CA. 94040 650-940-7038 On Mon, Nov 21, 2011 at 10:01 AM, wrote: > Send Histonet mailing list submissions to > ? ? ? ?histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ? ? ? ?http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ? ? ? ?histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ? ? ? ?histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ? 1. Senior Research Assistant Position in Histology (Esther C Peters) > ? 2. Handling Muscle, Nerves and Renal biopsy specimens (Nancy Schmitt) > ? 3. RE: Handling Muscle, Nerves and Renal biopsy specimens > ? ? ?(Galbraith, Joe) > ? 4. Chemical Recycling - B/R Instruments (Davide Costanzo) > ? 5. Per Diem work in LA - Beverly Hills area (Davide Costanzo) > ? 6. Re: Chemical Recycling - B/R Instruments (Rene J Buesa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 20 Nov 2011 19:44:14 -0500 > From: Esther C Peters > Subject: [Histonet] Senior Research Assistant Position in Histology > To: histonet@lists.utsouthwestern.edu > Cc: Patricia Latham > Message-ID: > Content-Type: text/plain; charset=windows-1252 > > Please see the following job announcement for a Senior Research Assistant at George Washington University in Washington, DC. > > Contact Dr. Patricia Latham at GWU if you are interested in this position: platham@mfa.gwu.edu > > Full-time Research Assistant - Histotechnologist > ? ? ? ?A position is available for a histotechnologist in a new research core laboratory in the GWU School of Biomedical Sciences. This person will be expected to maintain a histology laboratory, including ordering of supplies, washing glassware and maintaining equipment for optimal function, daily logs with inventory of specimens and blocks, labeling of samples and slides, tissue processing and embedding, microtome and cryostat sections, routine stains, special stains and immunostains with appropriate controls, as required to meet the needs of researchers at GWU. This person will need to work independently to deliver excellent quality results in a timely manner. The person should have strong communication skills and managerial skills, since there will be a need to work with researchers in several labs and to maintain inventory from several sources, to keep track of the flow of specimens and charges. The position will require data entry into an audit-trail database and the mainten > ance of meticulous records, with an exchange of data to the research center off-site and to labs at international sites. This person should have some experience in laboratory research, implementation of new techniques and the ability to trouble-shoot problems that might arise in this setting. Ideally, the person will have an entreprenurial spirit, since the success of the lab will determine its future existence. The full-time position is assured for 2 years, but it can be extended and/ or grow to full-time, if the lab is successful. > > Supervision: > The work will be supervised by a Pathologist as Project Manager. > > Entry level qualifications: > ? ? ? ? Bachelor?s degree in a clinical science with a course in Histology or equivalent combination of training and experience. > ? ? ? ? Experience working in a research setting. > ? ? ? ? Favored is eligibility or certification as a Histotechnologist or Histotechnician by the American Society for Clinical Pathology. > > > Esther C. Peters, Ph.D. > Assistant Professor > Department of Environmental Science & Policy > Biology Program/Medical Technology Coordinator > George Mason University > 4400 University Drive, MSN 5F2 > Fairfax, VA 22030-4444 > Office: David King Hall 3057 > Phone: 703-993-3462 > Fax: 703-993-1066 > epeters2@gmu.edu > > > > ------------------------------ > > Message: 2 > Date: Mon, 21 Nov 2011 14:54:38 +0000 > From: Nancy Schmitt > Subject: [Histonet] Handling Muscle, Nerves and Renal biopsy specimens > To: "histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<906B4DA90ED1DB4DB6C7E94D7CEE6C367B97FB@PEITHA.wad.pa-ucl.com> > Content-Type: text/plain; charset="us-ascii" > > These cases are only scheduled during regular hours M-F. > > Nancy > > ----------------------------------------------------- > Message: 1 > Date: Sun, 20 Nov 2011 00:34:44 -0500 > From: ADESUPO ADESUYI > Subject: [Histonet] Handling Muscle, Nerves and Renal biopsy specimens > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > > > ?Hi, > > ? ? Please I am wondering if you guys could help me by telling me how you handle specimens like Muscle, Nerve and Renal biopsy after the working hours like may be at night or weekend. > > ? Thank you all for your usual cooperation. > > ?Ade. > > > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > > ------------------------------ > > Message: 3 > Date: Mon, 21 Nov 2011 15:14:58 +0000 > From: "Galbraith, Joe" > Subject: [Histonet] RE: Handling Muscle, Nerves and Renal biopsy > ? ? ? ?specimens > To: Nancy Schmitt , > ? ? ? ?"histonet@lists.utsouthwestern.edu" > ? ? ? ? > Message-ID: > ? ? ? ?<6DC87DEA9229894DB3A09F8B61717A4610724977@hc-mailboxc1-n3.healthcare.uiowa.edu> > > Content-Type: text/plain; charset="us-ascii" > > Ade: > > I would agree with Nancy on muscle and nerve biopsies but not for renal biopsies. ?We have a tech on call to handle 'emergent' renal/liver biopsies on the weekends and holidays. These do happen rather frequently outside of regular business hours at my institution. ?How you choose to handle these will depend on the frequency and urgency of the situation at your institution. ?You can send me a separate email if you want more details. > > Joe > joseph-galbraith@uiowa.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt > Sent: Monday, November 21, 2011 8:55 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Handling Muscle, Nerves and Renal biopsy specimens > > These cases are only scheduled during regular hours M-F. > > Nancy > > ----------------------------------------------------- > Message: 1 > Date: Sun, 20 Nov 2011 00:34:44 -0500 > From: ADESUPO ADESUYI > Subject: [Histonet] Handling Muscle, Nerves and Renal biopsy specimens > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > > > ?Hi, > > ? ? Please I am wondering if you guys could help me by telling me how you handle specimens like Muscle, Nerve and Renal biopsy after the working hours like may be at night or weekend. > > ? Thank you all for your usual cooperation. > > ?Ade. > > > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ________________________________ > Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. ?If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. ?Please reply to the sender that you have received the message in error, then delete it. ?Thank you. > ________________________________ > > > > ------------------------------ > > Message: 4 > Date: Mon, 21 Nov 2011 09:41:22 -0800 > From: Davide Costanzo > Subject: [Histonet] Chemical Recycling - B/R Instruments > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=ISO-8859-1 > > Can anyone provide me information on B/R Instruments Pro series recyclers? > I am considerning them vs. CBG Biotech. I have heard rumors that the B/R > units throw a lot of heat from the lower boiler and damage the underlying > flooring. Can anyone confirm this, or any other troubles with their units? > Thank you. > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > > > ------------------------------ > > Message: 5 > Date: Mon, 21 Nov 2011 09:44:18 -0800 > From: Davide Costanzo > Subject: [Histonet] Per Diem work in LA - Beverly Hills area > To: histonet@lists.utsouthwestern.edu > Message-ID: > ? ? ? ? > Content-Type: text/plain; charset=ISO-8859-1 > > Hello all - > > We are opening a histology laboratory in the Beverly Hills area, and are in > need of per diem histotech's to cover vacation/sickness or upticks in > volume. If anyone in the LA area is interested in doing occassional work > for a few hours at a time to supplement their current job please contact > me, not by reply here but at my office email : ?david@blufrogpath.com > > Thank you. Happy Thanksgiving to all. > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > > > ------------------------------ > > Message: 6 > Date: Mon, 21 Nov 2011 09:47:11 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Chemical Recycling - B/R Instruments > To: histonet@lists.utsouthwestern.edu, Davide Costanzo > ? ? ? ? > Message-ID: > ? ? ? ?<1321897631.31877.YahooMailClassic@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > My B/R xylene/alcohol recycling unit was placed over a counter and did not cause any damage to it?after several years of continuous and reliable usage. > It?paid itself in 3 years from savings of xylene that I did not have to buy. > I do not know about the other brand you are asking about, but I do not?think it will better than B/R > Ren? J. > > --- On Mon, 11/21/11, Davide Costanzo wrote: > > > From: Davide Costanzo > Subject: [Histonet] Chemical Recycling - B/R Instruments > To: histonet@lists.utsouthwestern.edu > Date: Monday, November 21, 2011, 12:41 PM > > > Can anyone provide me information on B/R Instruments Pro series recyclers? > I am considerning them vs. CBG Biotech. I have heard rumors that the B/R > units throw a lot of heat from the lower boiler and damage the underlying > flooring. Can anyone confirm this, or any other troubles with their units? > Thank you. > > -- > *David Costanzo, MHS, PA (ASCP)* > Project Manager > *Blufrog Path Lab Solutions* > 9401 Wilshire Blvd. Ste 650 > Beverly Hills, CA 90212 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 96, Issue 32 > **************************************** > From relia1 <@t> earthlink.net Mon Nov 21 13:14:50 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Nov 21 13:14:57 2011 Subject: [Histonet] RELIA Hot Histology Job Alert - Histotech and Histology Supervisor needed North of NYC needed. Can you help? Message-ID: Hi Histonetters!! I have some hot new jobs to tell you about. I am working with a great client who is in need of a Histo Tech and a Histology Manager in NY just north of NYC. NYS License or eligible and 3 plus years experience required. These are full time permanent positions. I know this is a crazy busy time of year for all of us and this client is willing to move forward now or wait until after the holidays whatever is more convenient for you. For more info contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From mhale <@t> carisls.com Mon Nov 21 13:43:06 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Mon Nov 21 13:43:32 2011 Subject: [Histonet] Position In Louisiana Message-ID: <6F33D8418806044682A391273399860F0AA776DF@s-irv-ex301.PathologyPartners.intranet> Please no Recruiters only interested applicants : Great opportunity for a Histotechnician in a brand new laboratory! Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice located in Lafayette, Louisiana, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From mhale <@t> carisls.com Mon Nov 21 13:44:24 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Mon Nov 21 13:44:42 2011 Subject: [Histonet] AZ Position Message-ID: <6F33D8418806044682A391273399860F0AA776E7@s-irv-ex301.PathologyPartners.intranet> Please no recruiters' interested applicant only : Great opportunity for a Histotechnician in Scottsdale , AZ . Colon and Digestive Health Specialists is looking for a certified HT or HTL to run their laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours up to 25 hours a week . Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 From mward <@t> wakehealth.edu Mon Nov 21 15:28:17 2011 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Mon Nov 21 15:28:28 2011 Subject: [Histonet] IDH1 and IDH2 antibodies, MHC-Class I Message-ID: Hello all, I have had a request from our neuropathologists to bring these antibodies in house but I am having some trouble finding vendors for them. I would appreciate any information. Thanks! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 From Ronald.Houston <@t> nationwidechildrens.org Mon Nov 21 15:36:04 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Nov 21 15:36:17 2011 Subject: [Histonet] RE: IDH1 and IDH2 antibodies, MHC-Class I In-Reply-To: References: Message-ID: http://www.dianova.com/en/ Good luck... Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Martha Ward-Pathology [mward@wakehealth.edu] Sent: Monday, November 21, 2011 4:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IDH1 and IDH2 antibodies, MHC-Class I Hello all, I have had a request from our neuropathologists to bring these antibodies in house but I am having some trouble finding vendors for them. I would appreciate any information. Thanks! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From rjbuesa <@t> yahoo.com Mon Nov 21 15:44:06 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 21 15:44:14 2011 Subject: [Histonet] recycling solvents In-Reply-To: <84B344439E2C4E838577648AF1D47D03@dielangs.at> Message-ID: <1321911846.1162.YahooMailClassic@web65709.mail.ac4.yahoo.com> Hi Gudrun: I never recycled ethanol because it takes too long, uses too much energy, and the final product is never 100% pure. On the other hand I recycled xylene which a less time consuming operation, cheaper and renders a product that can be used directly in the tissue processor. I used to recycle an average of 2,782 liters/year (about 11 liters/day). The re-payment ratio was $537/month and at that rate the instrument (a B/R recycler) paid itself in 30 months. The disadvantage is that you have to protect yourself from xylene fumes during recycling and this is one of the reasons why xylene should be totally substituted by 2-propanol in the general operation of the lab. Ren? J. --- On Mon, 11/21/11, Gudrun Lang wrote: From: Gudrun Lang Subject: [Histonet] recycling solvents To: histonet@lists.utsouthwestern.edu Date: Monday, November 21, 2011, 1:49 PM Hi all! What is the general opinion about recycling ethanol and xylen?? Experiences? Reasonable volumes? I would be glad about any comment. Regards Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wilson6848 <@t> yahoo.com Mon Nov 21 18:44:00 2011 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Mon Nov 21 18:44:03 2011 Subject: [Histonet] Looking for Position in IHC/HISTOLOGY In-Reply-To: <1309821326.26450.YahooMailRC@web120902.mail.ne1.yahoo.com> References: <1309821326.26450.YahooMailRC@web120902.mail.ne1.yahoo.com> Message-ID: <1321922640.8772.YahooMailNeo@web120913.mail.ne1.yahoo.com> ?? ?I am an HTL(ASCP)QIHC Certified Histotech with an extensive experience in IHC. I am looking for position in IHC/HISTOLOGY.? Willing to relocate. ?Thanks,?Wilson. From mhale <@t> carisls.com Mon Nov 21 19:50:06 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Mon Nov 21 19:50:23 2011 Subject: [Histonet] Great Opportunity - Caris Life Sciences- Irving, TX Message-ID: <6F33D8418806044682A391273399860F0AA77B3A@s-irv-ex301.PathologyPartners.intranet> Please no recruiters'. Interested applicant only : Great Opportunity- TC/PC Manager position with Caris Life Sciences- Irving, TX. The TC/PC Manager will support the TCPC operations; this role will travel to customer sites and assist in building and setting up of laboratories. Assist in the maintenance and preparation for regulatory inspections (CAP, CLIA, etc.). Travel up to 70% of the time. Must have Excellent communication and project management skills. Knowledge, Skills, and Experience 1. 7-10 years experience working in Histology must have prior supervisor experience. 2. Vast knowledge of Histology lab equipment, purchasing, installation, set up and operation. 3. Knowledge of CLIA, CAP and other laboratory licensure processes and regulations. 4. Ability to consult on laboratory workflow and processes for various sized laboratory operations. 5. Must have strong QC/QA background in laboratory operations. 6. Experience with policies and procedures for Histology Laboratories 7. Previously served as a lab inspector a plus. Must have the ability to inspect and prepare laboratories for audits and inspections. 8. Ability to interview and evaluate technical experience of Histology technicians. 9. Flexibility for last minute travel changes to meet client expectations and needs. 10. Strong troubleshooting and problem solving skills is a must. 11. Knowledge or prior experience of building of Histology Laboratories a plus. 12. Experience in project management capacity, including all aspects of process development and execution. 13. Strong familiarity with project management software, such as MS Project 14. Solid working knowledge of current TC/PC environment for GI, DERM, UROLOGY and HEME Please contact Crystal Flowers at cflowers@carisls.com for additional information. Thanks! Crystal Flowers-Graves Manager, Corporate Recruiting Caris Life Sciences 6655 N. MacArthur Blvd. Irving, TX 75039 (214) 596-7003 Direct (214) 596-7490 Fax cflowers@carisls.com www.carisdx.com From yolanda.davies <@t> uct.ac.za Tue Nov 22 03:14:01 2011 From: yolanda.davies <@t> uct.ac.za (Yolanda Davies) Date: Tue Nov 22 03:14:19 2011 Subject: [Histonet] demonstration of asbestos by means of electron microscopy Message-ID: <4ECB83F902000022000A58DD@gwiasmtp.uct.ac.za> Dear all I am a histotechnologist in forensics, Cape Town, South Africa. I received a request to show asbestos in lung tissue where there is definite interstitial fibrosis, but the presence of asbestos is not clear. Is it possible to reveal asbestos by means of electron microscopy? Usually asbestos is demonstrated using the Perl's Prussian blue technique, but most times they are elusive. Could it be because of the sampling site or simply the nature of the asbestos? Thank you in advance Yolanda Davies Department of Forensic Medicine and Toxicology Falmouth building Anzio Road Observatory Cape Town South Africa ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### From lpwenk <@t> sbcglobal.net Tue Nov 22 03:56:40 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Nov 22 03:56:50 2011 Subject: [Histonet] demonstration of asbestos by means of electron microscopy In-Reply-To: <4ECB83F902000022000A58DD@gwiasmtp.uct.ac.za> References: <4ECB83F902000022000A58DD@gwiasmtp.uct.ac.za> Message-ID: <7AEB7AEEFD354B1F8A7A55677715873E@HP2010> The StainsFile page has some techniques, using polarizing microscopes. http://stainsfile.info/StainsFile/stain/pigment/asbestos.htm Asbestos are usually very small fibers, and will not show up in every section. Cutting thicker sections sometimes helps. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: Yolanda Davies Sent: Tuesday, November 22, 2011 4:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] demonstration of asbestos by means of electron microscopy Dear all I am a histotechnologist in forensics, Cape Town, South Africa. I received a request to show asbestos in lung tissue where there is definite interstitial fibrosis, but the presence of asbestos is not clear. Is it possible to reveal asbestos by means of electron microscopy? Usually asbestos is demonstrated using the Perl's Prussian blue technique, but most times they are elusive. Could it be because of the sampling site or simply the nature of the asbestos? Thank you in advance Yolanda Davies Department of Forensic Medicine and Toxicology Falmouth building Anzio Road Observatory Cape Town South Africa ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MAUGER <@t> email.chop.edu Tue Nov 22 07:18:02 2011 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Tue Nov 22 07:20:50 2011 Subject: [Histonet] RE: IDH1 and IDH2 antibodies, MHC-Class I In-Reply-To: References: Message-ID: <443F5B475A9BF647AB962E834884EBAD27F34AD0F7@EX7CCRPW03V1.chop.edu> Martha, They are now sold through HistoBiotec in the USA. Jo Mauger -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Monday, November 21, 2011 4:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IDH1 and IDH2 antibodies, MHC-Class I Hello all, I have had a request from our neuropathologists to bring these antibodies in house but I am having some trouble finding vendors for them. I would appreciate any information. Thanks! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sherrineely <@t> hotmail.com Tue Nov 22 07:37:04 2011 From: sherrineely <@t> hotmail.com (sherri neely) Date: Tue Nov 22 07:37:13 2011 Subject: [Histonet] I am finally became Boss Message-ID: Hello... I miss you so much This is for your eyes only http://stereotyp.home.pl/profile/61DavidHarrison/ talk to you later From brett_connolly <@t> merck.com Tue Nov 22 08:13:17 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Nov 22 08:13:28 2011 Subject: [Histonet] demonstration of asbestos by means of electron microscopy In-Reply-To: <4ECB83F902000022000A58DD@gwiasmtp.uct.ac.za> References: <4ECB83F902000022000A58DD@gwiasmtp.uct.ac.za> Message-ID: Yolanda, Yes it is, TEM is often used for asbestos detection in lung tissue to identify chrysotile and/or crocidolite (mesothelioma-associated) asbestos fibers. There are a lot of publications out there... Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yolanda Davies Sent: Tuesday, November 22, 2011 4:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] demonstration of asbestos by means of electron microscopy Dear all I am a histotechnologist in forensics, Cape Town, South Africa. I received a request to show asbestos in lung tissue where there is definite interstitial fibrosis, but the presence of asbestos is not clear. Is it possible to reveal asbestos by means of electron microscopy? Usually asbestos is demonstrated using the Perl's Prussian blue technique, but most times they are elusive. Could it be because of the sampling site or simply the nature of the asbestos? Thank you in advance Yolanda Davies Department of Forensic Medicine and Toxicology Falmouth building Anzio Road Observatory Cape Town South Africa ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From rjbuesa <@t> yahoo.com Tue Nov 22 08:28:29 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 22 08:28:37 2011 Subject: [Histonet] demonstration of asbestos by means of electron microscopy In-Reply-To: <4ECB83F902000022000A58DD@gwiasmtp.uct.ac.za> Message-ID: <1321972109.22669.YahooMailClassic@web65712.mail.ac4.yahoo.com> Many years ago asbestos fibers were demonstrated in hypochlorite digested tissues. The remaining material was filtered through Milllipore filter, washed and the fibers observed with polarized light. The Perl's method will stain no the asbestos fibers (that cannot be stained) but a reactive capsule the tissues secrete around the fiber, so it is an indirect method based in: if there is a tissue reaction to asbestos, they should have been present at a certain moment. As to its demonstration with EM I do not know but I think it will be very difficult to infiltrate?the fibers in Epoxi resin to be cut. If you are able to do that: how are you going to identify an extremely thin section of asbestos? I think that you should either try the tissue digestion or give another try at the Perl reaction. Ren? J. --- On Tue, 11/22/11, Yolanda Davies wrote: From: Yolanda Davies Subject: [Histonet] demonstration of asbestos by means of electron microscopy To: histonet@lists.utsouthwestern.edu Date: Tuesday, November 22, 2011, 4:14 AM Dear all I am a histotechnologist in forensics, Cape Town, South Africa. I received a request to show asbestos in lung tissue where there is definite interstitial fibrosis, but the presence of asbestos is not clear. Is it possible to reveal asbestos by means of electron microscopy? Usually asbestos is demonstrated using the Perl's Prussian blue technique, but most times they are elusive. Could it be because of the sampling site or simply the nature of the asbestos? Thank you in advance Yolanda Davies Department of Forensic Medicine and Toxicology Falmouth building Anzio Road Observatory Cape Town South Africa ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Nov 22 08:43:12 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Nov 22 08:43:28 2011 Subject: [Histonet] Asbestos Body Digestion Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640845443480@CHEXCMS10.one.ads.che.org> To the person who asked about asbestos.. Not sure if digestion would have anything to do with what you are trying to do, but Dr. Victor Roggli at Duke performs this procedure (and interprets the results). See http://pathology.mc.duke.edu/website/WebForm.aspx?id=PulmonaryPathMain He might be a good resource for you. Best, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From pathologylab <@t> ymail.com Tue Nov 22 09:13:21 2011 From: pathologylab <@t> ymail.com (Pathology Lab) Date: Tue Nov 22 09:13:27 2011 Subject: [Histonet] UNSUSCRIBE Message-ID: <1321974801.6021.YahooMailNeo@web121406.mail.ne1.yahoo.com> ? Lcda. Mary V. Guerrero,BS, MBA,HtL ? ? ? ? ? ? ? Administradora/Coordinadora General ?Pathology Lab.? ? 55?N. Dr. Basora Edificio M?dico IV?Oficina 206 Mayaguez, Puerto Rico 00680 Tel. 787-834-8202 ?Fax: 787-831-5255 Sra. Dimary Valent?n?????????? Sra. Iris Franqui??????????????? Sra. Myrna Gonz?lez ? Facturaci?n???????? Reportes???????? ? ? Transcripcion This email may contain confidential health information that is legally privileged.? This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled.? If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited.? If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . From hhawkins <@t> UTMB.EDU Tue Nov 22 10:48:50 2011 From: hhawkins <@t> UTMB.EDU (Hawkins, Hal K.) Date: Tue Nov 22 10:48:54 2011 Subject: [Histonet] part Message-ID: <22624908330375439D6382C9F95093FF67EFA3@GRMBX1.utmb.edu> I just learned that the heating element for the paraffin bath on our old Shandon embedding center has failed, and the company no longer supports the older models. Does anyone have a source for that part they could recommend? Many thanks Hal Hawkins UTMB Galveston and Shriners Hospital From gu.lang <@t> gmx.at Tue Nov 22 10:56:55 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Nov 22 10:57:00 2011 Subject: AW: [Histonet] recycling solvents In-Reply-To: <84B344439E2C4E838577648AF1D47D03@dielangs.at> References: <84B344439E2C4E838577648AF1D47D03@dielangs.at> Message-ID: <4170766ED6F14AB29B7BF0DA48E40117@dielangs.at> Thanks to all who answered my question. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Montag, 21. November 2011 19:50 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] recycling solvents Hi all! What is the general opinion about recycling ethanol and xylen? Experiences? Reasonable volumes? I would be glad about any comment. Regards Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SteveM <@t> mcclainlab.com Tue Nov 22 11:45:16 2011 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Tue Nov 22 11:45:02 2011 Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 33 xylene and ethanol recycling in lab Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF169784@ML1.McClainLabs.local> Rene, I am interested to learn more of your experience with isopropanol as a clearing agent. Does it take longer to clear than xylene? For processing how many stations and how long are the times you use for clearing? How often do you maintain or change isopropanol - how many blocks per liter? What about de-paraffinization or running down slides to water- what times are you using? Are you recycling isopropanol ? Having seen the devastating effects of a histolab fire during residency (Vermont) due to dioxane (flashpoint 18 degrees C), I am leery of isopropanol alcohols low flashpoint of 12 degrees C (vs 30 for xylene). Does that concern you? Yes xylene's properties, whether toxicity or carcinogenicity and flammability need to be considered, but it is still a valuable reagent, especially when one considers it can be recycled in the lab. Techs need to be trained for the safe handling and monitoring badges worn. The gasoline you pump at the gas station has xylene, benzene, and worse. 1. Modern recyclers (within the past 7-10 years) are computer controlled and when vented, xylene can be safely recycled (By vented I mean when connected to the same external fan-driven vents as the processors). (If your lab is not vented, you should work on that first before tackling recycling -my opinion) 2. We have good experience with BR- CBGB is a reputable company also. My experience is with the modern computer-controlled 5 gallon models ; the 20 year old glass-bottomed units held only 2 gallons if memory serves me. a) we mounted our 2 retorts on plastic garbage can wheels with 2 plates of regular 3/4 inch plywood so the retorts can be easily swapped in and out of the still; separate retorts for xylene and alcohol. I see no damage underneath after 7 years, either from heat or xylene. 3. Ethanol does take longer to recycle, and without strict attention the recycled alcohol may not be sufficiently pure- one can easily end up with 85-90% alcohol rather than 95%. (see part 6 below) 4. The critical features for alcohol recycling are a) the alcohols being recycled should be reasonably clean and relatively concentrated and contain NO xylene at all; For alcohol we only recycle the 95 and 100% alcohols before the xylenes on the processors and stainers; xylene-contaminated alcohols do not work. The rule of thumb I learned is that xylene can be distilled from xylene-alcohol mixtures, but not the other way around. b) the recycling program being run should be tailored and adjusted to compensate for the purity of the alcohols. c. The purity of the product needs to be monitored, e.g., we check the specific gravity, do the 50:50 mixing test, and send samples for analysis quarterly. d) It takes longer to recycle alcohols- maybe 6-8 hours. 5.The statement about energy required for recycling ethanol is far off the mark in my experience. To recycle 1 gallon of alcohol is about $0.40 or 1/50th of the waste expense to cart away 1 gallon ($18-20/gallon in NYS). 6. I have no experience yet I am told that isopropanol alcohols can be recycled, but may be even more finicky than reagent ethanol-methanol distillation. The chemistry suggests this is likely to be true. For example, ethanol is a 4.5% azeotrope with water, meaning the maximum concentration one can distill to is 95.5%. Isopropanol is a 12.1% azeotrope, meaning once it is diluted/contaminated with more than 13% water, the maximum concentration recoverable is 87.9%. Depending on how the isopropanol is used in the lab, recycled 87% isopropanol from the recycler may be less than suitable for your needs. I suppose if one recycled only the concentrated, less-contaminated isopropanol it might pretty well. See http://en.wikipedia.org/wiki/Azeotrope_(data) Steve A. McClain, MD 631 361 4000 Hi Gudrun: I never recycled ethanol because it takes too long, uses too much energy, and the final product is never 100% pure. On the other hand I recycled xylene which a less time consuming operation, cheaper and renders a product that can be used directly in the tissue processor. I used to recycle an average of 2,782 liters/year (about 11 liters/day). The re-payment ratio was $537/month and at that rate the instrument (a B/R recycler) paid itself in 30 months. The disadvantage is that you have to protect yourself from xylene fumes during recycling and this is one of the reasons why xylene should be totally substituted by 2-propanol in the general operation of the lab. Ren? J. From Marilyn.A.Weiss <@t> kp.org Tue Nov 22 12:01:12 2011 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Tue Nov 22 12:05:38 2011 Subject: [Histonet] out of office at noon today Message-ID: I will be out of the office starting 11/22/2011 and will not return until 11/28/2011. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. I will be in town for some of the time. Thank you. From Nancy_Schmitt <@t> pa-ucl.com Tue Nov 22 12:05:33 2011 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue Nov 22 12:05:41 2011 Subject: [Histonet] Chemical Recycling - B/R Instruments(Davide Costanzo) /recycling solvents In-Reply-To: <20111122151828.83E131BF9D8@mail.pa-ucl.com> References: <20111122151828.83E131BF9D8@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B9AE8@PEITHA.wad.pa-ucl.com> We use the CBG recycling system - we have 1 for formalin and 1 for alcohol and xylene. They are workhorses and we have had very little issue with them. Our solvent recycler is run every day - we rotate a week at a time between xylene and alcohol. ---------------------------------------------------------------- Can anyone provide me information on B/R Instruments Pro series recyclers? > I am considerning them vs. CBG Biotech. I have heard rumors that the B/R > units throw a lot of heat from the lower boiler and damage the underlying > flooring. Can anyone confirm this, or any other troubles with their units? > Thank you What is the general opinion about recycling ethanol and xylen? Experiences? Reasonable volumes? NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From hrfulklab <@t> gmail.com Tue Nov 22 13:02:47 2011 From: hrfulklab <@t> gmail.com (H R) Date: Tue Nov 22 13:02:51 2011 Subject: [Histonet] CLIA certification for grossing Message-ID: I have seen a couple of job postings that listed CLIA certification for grossing as a requirement. Can someone give me some information on that certification. I am currently doing gross in our derm office and I need to know if I need to be certified. Thanks HR From joelleweaver <@t> hotmail.com Tue Nov 22 13:25:31 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Nov 22 13:25:35 2011 Subject: [Histonet] CLIA certification for grossing In-Reply-To: References: Message-ID: This links to an article since the language of the regulation is dense, hits the high points. http://laboratory-manager.advanceweb.com/Features/Article-2/The-Grossing-Histotechnologist-in-Surgical-Pathology.aspx Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > Date: Tue, 22 Nov 2011 14:02:47 -0500 > From: hrfulklab@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CLIA certification for grossing > > I have seen a couple of job postings that listed CLIA certification for > grossing as a requirement. Can someone give me some information on that > certification. I am currently doing gross in our derm office and I need to > know if I need to be certified. > Thanks > HR > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Nov 22 14:21:30 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 22 14:21:33 2011 Subject: [Histonet] xylene and ethanol recycling in lab In-Reply-To: <012ADA4B5CC00F4AB5E4BAA399E0A5DF169784@ML1.McClainLabs.local> Message-ID: <1321993290.46393.YahooMailClassic@web65703.mail.ac4.yahoo.com> Steve: Of all the concerns you have, the fundamental should be that xylene is very, very dangerous from the health point of view. Under separate cover I am sending you 3 articles that answer your fundamental questions. Ah, by the way, you do not need xylene to dewax sections, just use a 2% aq. solution of dishwashesoapap at 90?C (this is also included).Ren? J. --- On Tue, 11/22/11, Steve McClain wrote: From: Steve McClain Subject: RE: Histonet Digest, Vol 96, Issue 33 xylene and ethanol recycling in lab To: "histonet@lists.utsouthwestern.edu" Cc: "rjbuesa@yahoo.com" Date: Tuesday, November 22, 2011, 12:45 PM Rene, I am interested to learn more of your experience with isopropanol? as a clearing agent. Does it take longer to clear than xylene? For processing how many stations and how long are the times you use for clearing? How often do you maintain or change isopropanol? - how many blocks per liter? What about de-paraffinization or running down slides to water- what times are you using? Are you recycling isopropanol ? Having seen the devastating effects of a histolab fire during residency (Vermont) due to dioxane (flashpoint 18 degrees C), I am leery of isopropanol? alcohols? low flashpoint of 12 degrees C (vs 30 for xylene).? Does that concern you? Yes xylene's properties, whether toxicity or carcinogenicity and flammability need to be considered, but it is still a valuable reagent, especially when one considers it can be recycled in the lab. Techs need to be trained for the safe handling and monitoring badges worn. The gasoline you pump at the gas station has xylene, benzene, and worse. 1. Modern recyclers (within the past 7-10 years) are computer controlled and when vented, xylene can be safely recycled (By vented I mean when connected to the same external fan-driven vents as the processors). (If your lab is not vented, you should work on that first before tackling recycling -my opinion) 2. We have good experience with BR- CBGB is a reputable company also.? My experience is with the modern computer-controlled 5 gallon models ; the 20 year old glass-bottomed units held only 2 gallons if memory serves me. a) we mounted our 2 retorts on plastic garbage can wheels with 2 plates of regular 3/4 inch plywood so the retorts can be easily swapped in and out of the still; separate retorts for xylene and alcohol.? I see no damage underneath after 7 years, either from heat or xylene. 3. Ethanol does take longer to recycle, and without strict attention the recycled alcohol may not be sufficiently pure- one can easily end up with 85-90% alcohol rather than 95%.? (see part 6 below) 4. The critical features for alcohol recycling are a) the alcohols being recycled should be reasonably clean and relatively concentrated and contain NO xylene at all; For alcohol we only recycle the 95 and 100% alcohols before the xylenes on the processors and stainers; xylene-contaminated alcohols do not work.? The rule of thumb I learned is that xylene can be distilled from xylene-alcohol mixtures, but not the other way around. b) the recycling program being run should be tailored and adjusted? to compensate for the purity of the alcohols. c. The purity of the product needs to be monitored, e.g., we check the specific gravity, do the 50:50 mixing test, and send samples for analysis quarterly. d) It takes longer to recycle alcohols- maybe 6-8 hours. 5.The statement about energy required for recycling ethanol is far off the mark in my experience. To recycle 1 gallon of alcohol is about $0.40 or 1/50th of the waste expense to cart away 1 gallon ($18-20/gallon in NYS).? 6.? I have no experience yet I am told that isopropanol? alcohols? can be recycled, but may be even more finicky than reagent ethanol-methanol distillation.? The chemistry suggests this is likely to be true.? For example, ethanol is a 4.5% azeotrope with water, meaning the maximum concentration one can distill to is 95.5%. Isopropanol? is a 12.1% azeotrope, meaning once it is diluted/contaminated with more than 13% water, the maximum concentration recoverable is 87.9%. Depending on how the isopropanol is used in the lab, recycled 87% isopropanol from the recycler may be less than suitable for your needs.? I suppose if one recycled only the concentrated, less-contaminated isopropanol it might pretty well. See http://en.wikipedia.org/wiki/Azeotrope_(data) Steve A. McClain, MD 631 361 4000 Hi Gudrun: I never recycled ethanol because it takes too long, uses too much energy, and the final product is never 100% pure. On the other hand I recycled xylene which a less time consuming operation, cheaper and renders a product that can be used directly in the tissue processor. I used to recycle an average of 2,782 liters/year (about 11 liters/day). The re-payment ratio was $537/month and at that rate the instrument (a B/R recycler) paid itself in 30 months. The disadvantage is that you have to protect yourself from xylene fumes during recycling and this is one of the reasons why xylene should be totally substituted by 2-propanol in the general operation of the lab. Ren? J. From Luis.Chiriboga <@t> nyumc.org Tue Nov 22 14:53:55 2011 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Tue Nov 22 14:54:00 2011 Subject: [Histonet] Fibulin 3 Message-ID: Does anyone have any experience with Fibulin 3 (EFEMP1) IHC in human tissues? Interested in opinions/observations on staining patterns and sub-cellular localization Thanks in advance and happy thanksgiving to all Luis Chiriboga Ph.D, Director OCS Experimental Pathology IHC Core Lab NYU School of Medicne Department of Pathology 4w27 (212) 562-4667 Luis.Chiriboga@nyumc.org ------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
================================= 
From CIngles <@t> uwhealth.org  Tue Nov 22 15:37:31 2011
From: CIngles <@t> uwhealth.org (Ingles Claire )
Date: Tue Nov 22 15:39:16 2011
Subject: [Histonet] Fibulin 3
References: 
Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A792@UWHC-MAIL2.uwhis.hosp.wisc.edu>

Does this antibody detect little lies?
 
Sorry, short week and am anticipating the turkey!
Claire
 
 

Subject: [Histonet] Fibulin 3

Does anyone have any experience with Fibulin 3 (EFEMP1) IHC in human tissues? Interested in opinions/observations on staining patterns  and sub-cellular localization
Thanks in advance and happy thanksgiving to all





From turkekul <@t> gmail.com  Tue Nov 22 15:53:09 2011
From: turkekul <@t> gmail.com (mesruh turkekul)
Date: Tue Nov 22 15:53:14 2011
Subject: [Histonet] thick paraffin sections
Message-ID: 

Dear Friends,


I am trying to section 25-50um thick paraffin sections of mouse brain. I
had success with 40um thick tumor sections but brain is very difficult to
section as such thick sections. I always use ParaPlast plus melting at 56 C.
Any suggestions on how to cut thick paraffin sections? Is low melting
paraffin going to help?

Thanks,


Happy holidays!

Mesruh Turkekul
mskcc.org
From cmarievernick <@t> hotmail.com  Tue Nov 22 23:41:31 2011
From: cmarievernick <@t> hotmail.com (Corrie Vernick)
Date: Tue Nov 22 23:41:35 2011
Subject: [Histonet] Standard Operating Procedure H & E
Message-ID: 




 		 	   		  
From b-frederick <@t> northwestern.edu  Wed Nov 23 07:47:25 2011
From: b-frederick <@t> northwestern.edu (Bernice Frederick)
Date: Wed Nov 23 07:48:25 2011
Subject: [Histonet] RE: Fibulin 3
In-Reply-To: <064F1ACBAE8A78469AE2E41D533D87E505A792@UWHC-MAIL2.uwhis.hosp.wisc.edu>
References: 
	<064F1ACBAE8A78469AE2E41D533D87E505A792@UWHC-MAIL2.uwhis.hosp.wisc.edu>
Message-ID: <62C639732D3F274DACED033EBDF6ADAF1E191E81@evcspmbx3.ads.northwestern.edu>

Or does it detect only 3???

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick@northwestern.edu

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire 
Sent: Tuesday, November 22, 2011 3:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Fibulin 3

Does this antibody detect little lies?
 
Sorry, short week and am anticipating the turkey!
Claire
 
 

Subject: [Histonet] Fibulin 3

Does anyone have any experience with Fibulin 3 (EFEMP1) IHC in human tissues? Interested in opinions/observations on staining patterns  and sub-cellular localization Thanks in advance and happy thanksgiving to all





_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From bridget.maryott <@t> ventana.roche.com  Wed Nov 23 08:27:50 2011
From: bridget.maryott <@t> ventana.roche.com (Maryott, Bridget)
Date: Wed Nov 23 08:28:04 2011
Subject: [Histonet] RE: Brass cryostat chucks
Message-ID: 

I know I just saw a box of these in my garage. I will check over the weekend. I have a plethora of old microscope/microtome parts left over from my father that I have no clue what to do with anymore. 

-Bridget Maryott, HT (ASCP)
          Tissue Sample Management
          Ventana Medical Systems, Inc.
          a member of the Roche Group
          1910 E. Innovation Park Drive
          Tucson, Arizona  85755
 
          Tel: 520.229.4022
           Lab: 520.877.7145
          mailto: bridget.maryott@ventana.roche.com
 

Message: 9
Date: Thu, 17 Nov 2011 15:37:03 -0500
From: "Goodwin, Diana" 
Subject: [Histonet] Brass cryostat chucks
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID:
	<09411E0112A96A459D8D5FBDAB9C15C72480DAE2DF@HAMEXMBA.rwjham.local>
Content-Type: text/plain; charset="us-ascii"

Greeting, Histonetters.

I am in search of those brass cryostat chucks with the holes in them.  Any ideas?  Googled my brains out with no luck.


Diana G. Goodwin, BS, HT(ASCP)QIHC

Department of Pathology

Robert Wood Johnson University Hospital at Hamilton

One Hamilton Health Place

Hamilton, NJ  08690

Ph:  609.631.6996

Email:  dgoodwin@rwjuhh.edu




From cmarievernick <@t> hotmail.com  Wed Nov 23 08:52:03 2011
From: cmarievernick <@t> hotmail.com (Corrie Vernick)
Date: Wed Nov 23 08:52:11 2011
Subject: [Histonet] standard operating procedure H&E
Message-ID: 





Hi,
 
I am a student and I am working on an assignment writing a mock SOP for the H & E staining procedure, both progressive and regressive. One heading of the SOP is control. I read the book and tried to google for a good answer as to what this part of the SOP entails. I know that controls are run to make sure that the slides are staining properly before new reagents are used on patient tissue. I am wondering if  what I should be putting under the control heading of the SOP is that a control should be run after every stain or reagent change, or if there is something I am missing.
 
Thank you,
Corrinne Vernick
Keiser University
Orlando, FL U.S.A.
 		 	   		  
From sdysart <@t> mirnarx.com  Wed Nov 23 09:04:40 2011
From: sdysart <@t> mirnarx.com (Sarah Dysart)
Date: Wed Nov 23 09:04:52 2011
Subject: [Histonet] standard operating procedure H&E
In-Reply-To: 
References: 
Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D50020DF0@SN2PRD0702MB110.namprd07.prod.outlook.com>

Usually now I only run an H&E control when I get a new lot of hematoxylin or eosin to make sure the stain is good.  In other labs (especially if they are a strict GLP lab) I have run a control daily.  I think it is just dependent on how strict your QA department is.

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Corrie Vernick
Sent: Wednesday, November 23, 2011 8:52 AM
To: Histonet Lists Help
Subject: [Histonet] standard operating procedure H&E





Hi,
 
I am a student and I am working on an assignment writing a mock SOP for the H & E staining procedure, both progressive and regressive. One heading of the SOP is control. I read the book and tried to google for a good answer as to what this part of the SOP entails. I know that controls are run to make sure that the slides are staining properly before new reagents are used on patient tissue. I am wondering if  what I should be putting under the control heading of the SOP is that a control should be run after every stain or reagent change, or if there is something I am missing.
 
Thank you,
Corrinne Vernick
Keiser University
Orlando, FL U.S.A.
 		 	   		  _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From mward <@t> wakehealth.edu  Wed Nov 23 09:06:58 2011
From: mward <@t> wakehealth.edu (Martha Ward-Pathology)
Date: Wed Nov 23 09:19:09 2011
Subject: [Histonet] MHC-class 1 antibody
Message-ID: 

Thank you to the people who responded to my inquiry about the IDH1 antibody.   I think we have found one to try.

I have also have a  request for the MHC-Class 1 antibody for muscle biopsies.    Could anyone performing this suggest a vendor.   So far I am coming up with antibodies for frozen tissue but they are looking for an antibody for paraffin embedded tissues.   Any help would be appreciated.

Hope everyone has a good Thanksgiving holiday.

Martha Ward, MT (ASCP) QIHC
Manager, Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27157
336-716-2104

From rjbuesa <@t> yahoo.com  Wed Nov 23 10:46:29 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Nov 23 10:46:32 2011
Subject: [Histonet] standard operating procedure H&E
In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D50020DF0@SN2PRD0702MB110.namprd07.prod.outlook.com>
Message-ID: <1322066789.22375.YahooMailClassic@web65715.mail.ac4.yahoo.com>

Running one H&E control at the start and finish of the day's staining was my standard procedure. I wanted to being able to determine how good the staining was at the start and end of the day's work.
Ren? J.

--- On Wed, 11/23/11, Sarah Dysart  wrote:


From: Sarah Dysart 
Subject: RE: [Histonet] standard operating procedure H&E
To: "Corrie Vernick" , "Histonet Lists Help" 
Date: Wednesday, November 23, 2011, 10:04 AM


Usually now I only run an H&E control when I get a new lot of hematoxylin or eosin to make sure the stain is good.? In other labs (especially if they are a strict GLP lab) I have run a control daily.? I think it is just dependent on how strict your QA department is.

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas? 78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Corrie Vernick
Sent: Wednesday, November 23, 2011 8:52 AM
To: Histonet Lists Help
Subject: [Histonet] standard operating procedure H&E





Hi,

I am a student and I am working on an assignment writing a mock SOP for the H & E staining procedure, both progressive and regressive. One heading of the SOP is control. I read the book and tried to google for a good answer as to what this part of the SOP entails. I know that controls are run to make sure that the slides are staining properly before new reagents are used on patient tissue. I am wondering if? what I should be putting under the control heading of the SOP is that a control should be run after every stain or reagent change, or if there is something I am missing.

Thank you,
Corrinne Vernick
Keiser University
Orlando, FL U.S.A.
??? ???????? ?????? ??? ? _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From FUNKM <@t> mercyhealth.com  Wed Nov 23 11:09:08 2011
From: FUNKM <@t> mercyhealth.com (Marcia Funk)
Date: Wed Nov 23 11:09:18 2011
Subject: [Histonet] Standard work
Message-ID: <4ECCD454020000AC00009874@nodcdmg2.no.trinity-health.org>

 
        Hello- Please share with me if you have Standard Work in you
                  Histology lab.  We are looking at several systems for tracking
                  but we also want to implement Standard Work with audits.
                  This would include accessing, embedding sectioning, labeling
                  for the technical area.  Really appreciate your input as we 
                  follow through our process.  
                  Thanks Marcia
                 
 
 
Marcia Funk 
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-428-7907
From arvkevi <@t> gmail.com  Wed Nov 23 11:22:35 2011
From: arvkevi <@t> gmail.com (K)
Date: Wed Nov 23 11:22:45 2011
Subject: [Histonet] CISH image analysis
Message-ID: 

Does anyone know if there are any image analysis software available to analyze chromgenic in-situ hybridization (CISH), that are for in vitro diagnostics?

Thanks!



From susanbachus <@t> verizon.net  Wed Nov 23 11:31:41 2011
From: susanbachus <@t> verizon.net (Susan Bachus)
Date: Wed Nov 23 11:31:49 2011
Subject: [Histonet] RE: Brass cryostat chucks
In-Reply-To: 
References: 
Message-ID: <416EE38223634BC698C4FA43642ACD80@SusanBachusPC>

You got me to thinking, that I would be in a real jam if I ever lost mine! 
I'd rather buy one from someone who has old ones they don't need any more, 
but I did find some (at least I think this is what you're describing) 
available online at:
http://www.psl-equip.com/shop/advanced_search_result.php?keywords=CC-3330+&osCsid=683519442648223efdbb7a61f87d1c05
(You might have to "register" to see the information, but the part # is 
CC-3330 & they cost $22.)

-----Original Message----- 
From: Maryott, Bridget
Sent: Wednesday, November 23, 2011 9:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Brass cryostat chucks

I know I just saw a box of these in my garage. I will check over the 
weekend. I have a plethora of old microscope/microtome parts left over from 
my father that I have no clue what to do with anymore.

-Bridget Maryott, HT (ASCP)
          Tissue Sample Management
          Ventana Medical Systems, Inc.
          a member of the Roche Group
          1910 E. Innovation Park Drive
          Tucson, Arizona  85755

          Tel: 520.229.4022
           Lab: 520.877.7145
          mailto: bridget.maryott@ventana.roche.com


Message: 9
Date: Thu, 17 Nov 2011 15:37:03 -0500
From: "Goodwin, Diana" 
Subject: [Histonet] Brass cryostat chucks
To: "'histonet@lists.utsouthwestern.edu'"

Message-ID:
<09411E0112A96A459D8D5FBDAB9C15C72480DAE2DF@HAMEXMBA.rwjham.local>
Content-Type: text/plain; charset="us-ascii"

Greeting, Histonetters.

I am in search of those brass cryostat chucks with the holes in them.  Any 
ideas?  Googled my brains out with no luck.


Diana G. Goodwin, BS, HT(ASCP)QIHC

Department of Pathology

Robert Wood Johnson University Hospital at Hamilton

One Hamilton Health Place

Hamilton, NJ  08690

Ph:  609.631.6996

Email:  dgoodwin@rwjuhh.edu




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



From dmlongoria <@t> ecrmc.org  Wed Nov 23 12:39:24 2011
From: dmlongoria <@t> ecrmc.org (Diana Martinez-Longoria)
Date: Wed Nov 23 12:39:33 2011
Subject: [Histonet] Hema-Diff procedure
Message-ID: <12B71261212BE94BB9FB7735484B9FA119EF60@EXMBX01.ecrmc.ci.el-centro.ca.us>

We are trying to find out what other histology laboratories do with regards to the minutes/seconds for each step in the Hema-Diff stain? How many dips per each step? We would greatly appreciate it. Thanks!



Diana Martinez-Longoria

Histology Technician (ASCP)cm

El Centro Regional Medical Center




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??


From jsjurczak <@t> comcast.net  Wed Nov 23 12:55:03 2011
From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net)
Date: Wed Nov 23 12:55:21 2011
Subject: [Histonet] RE: Brass cryostat chucks
In-Reply-To: <416EE38223634BC698C4FA43642ACD80@SusanBachusPC>
Message-ID: <1902422846.183283.1322074502971.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net>

Precision Equipment and Design in South Carolina can make them for you. They have a machine shop and they make metal and plastic parts for microtomes, processors, etc. 

----- Original Message -----
From: "Susan Bachus"  
To: histonet@lists.utsouthwestern.edu 
Sent: Wednesday, November 23, 2011 11:31:41 AM 
Subject: Re: [Histonet] RE: Brass cryostat chucks 

You got me to thinking, that I would be in a real jam if I ever lost mine! 
I'd rather buy one from someone who has old ones they don't need any more, 
but I did find some (at least I think this is what you're describing) 
available online at: 
http://www.psl-equip.com/shop/advanced_search_result.php?keywords=CC-3330+&osCsid=683519442648223efdbb7a61f87d1c05 
(You might have to "register" to see the information, but the part # is 
CC-3330 & they cost $22.) 

-----Original Message----- 
From: Maryott, Bridget 
Sent: Wednesday, November 23, 2011 9:27 AM 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] RE: Brass cryostat chucks 

I know I just saw a box of these in my garage. I will check over the 
weekend. I have a plethora of old microscope/microtome parts left over from 
my father that I have no clue what to do with anymore. 

-Bridget Maryott, HT (ASCP) 
Tissue Sample Management 
Ventana Medical Systems, Inc. 
a member of the Roche Group 
1910 E. Innovation Park Drive 
Tucson, Arizona 85755 

Tel: 520.229.4022 
Lab: 520.877.7145 
mailto: bridget.maryott@ventana.roche.com 


Message: 9 
Date: Thu, 17 Nov 2011 15:37:03 -0500 
From: "Goodwin, Diana"  
Subject: [Histonet] Brass cryostat chucks 
To: "'histonet@lists.utsouthwestern.edu'" 
 
Message-ID: 
<09411E0112A96A459D8D5FBDAB9C15C72480DAE2DF@HAMEXMBA.rwjham.local> 
Content-Type: text/plain; charset="us-ascii" 

Greeting, Histonetters. 

I am in search of those brass cryostat chucks with the holes in them. Any 
ideas? Googled my brains out with no luck. 


Diana G. Goodwin, BS, HT(ASCP)QIHC 

Department of Pathology 

Robert Wood Johnson University Hospital at Hamilton 

One Hamilton Health Place 

Hamilton, NJ 08690 

Ph: 609.631.6996 

Email: dgoodwin@rwjuhh.edu 




_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
From MLunetta <@t> luhcares.org  Wed Nov 23 14:10:48 2011
From: MLunetta <@t> luhcares.org (Matthew Lunetta)
Date: Wed Nov 23 14:11:06 2011
Subject: [Histonet] Chemical Recycling - B/R
	Instruments(Davide/recycling solvents
Message-ID: <4ECCF0D8020000A80006B9E8@ns.luhcares.org>

We have been running the same B/R Instument for alcohol and xylene for the last 7-8 years. It is fantastic. We have not found any heat issues with it at all, It is run every day ussually two to three runs per day.

Matt Lunetta
BS HT(ASCP)
Longmont United Hospital


Message: 2
Date: Tue, 22 Nov 2011 18:05:33 +0000
From: Nancy Schmitt 
Subject: [Histonet] Chemical Recycling - B/R Instruments(Davide
Costanzo) /recycling solvents 
To: "histonet@lists.utsouthwestern.edu"

Message-ID:
<906B4DA90ED1DB4DB6C7E94D7CEE6C367B9AE8@PEITHA.wad.pa-ucl.com>
Content-Type: text/plain; charset="us-ascii"

We use the CBG recycling system - we have 1 for formalin and 1 for alcohol and xylene. They are workhorses and we have had very little issue with them. 
Our solvent recycler is run every day - we rotate a week at a time between xylene and alcohol.
----------------------------------------------------------------
Can anyone provide me information on B/R Instruments Pro series recyclers?
> I am considerning them vs. CBG Biotech. I have heard rumors that the B/R
> units throw a lot of heat from the lower boiler and damage the underlying
> flooring. Can anyone confirm this, or any other troubles with their units?
> Thank you


What is the general opinion about recycling ethanol and xylen? Experiences?
Reasonable volumes?



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From histotech411 <@t> gmail.com  Wed Nov 23 16:54:49 2011
From: histotech411 <@t> gmail.com (Jenny Vega)
Date: Wed Nov 23 16:54:54 2011
Subject: [Histonet] Question about Leica TP1020 and its tissue basket holders
Message-ID: 

Hello everyone. In my laboratory we have an old version of the Leica TP1020
tissue processor. My question is this. My supervisor told me that after the
machine finished processing the tissues on the next day, the tissue basket
was going to be in the last container which is going to be the second
container with paraffin wax. She told me that after removing the tissue
basket from the machine I had to rotate the tissue basket holder to it's
first station by pushing the the 'circle arrow' in the control panel which
is the first formalin container. In this machine the tissue basket holder
of both of the formalin containers are gray and the rest are black. (not
sure ALL of them are black but I know that the ones that are for the
paraffin containers are black).

I want to know if this is correct because when I go to the instruction
manuals and I go the "Removing the specimens" chapter it says


? Lift the carousel.
? Allow for the tissue basket to drain in that
position.
? Lift the tissue basket slightly with your hand
and pull it out of the basket holder in a horizontal
movement.
? Lower the carousel.

I got this manual online and its for the most recent Leica TP1020 machine
but it is still the same model. It doesnt says to rotate the tissue basket
holder to it's first station

When I put the tissue basket with new tissues I placed it in the first
station which is the first container of formalin, but what I don't remember
if  today I rotated the gray lid so it could be on top of the formalin
container. I think I did it in the morning but now I have my doubts. I have
always followed my supervisors instructions, what she told me makes sense
but I want to be sure if the processing cycle is going to be interrupted or
steps are going to be skipped if I don't return the "gray" tissue basket
holders to the formalin containers, and just leave it like the machine left
it after it finished the processing cycle. If you still placed the tissue
basket in the first formalin container would you still have issues or is
the machine going to follow the steps in order?


Thanks. I hope I do not sound confusing.
From mhardin <@t> carisls.com  Wed Nov 23 20:41:32 2011
From: mhardin <@t> carisls.com (Hardin, Michelle)
Date: Wed Nov 23 20:41:42 2011
Subject: [Histonet] Great Opportunity - Caris Life Sciences- Phoenix, AZ
Message-ID: 

Please no recruiters. Interested applicants only : 

 

Great Opportunity- Histology IHC Supervisor  position with Caris Life
Sciences- Irving, TX.

The IHC Supervisor will supervise a high performance team of
Histotechnicians and Lab Aides for a growing anatomic pathology
laboratory. This position ensures that all quality and turnaround time
standards are met. 

Responsibilities include ensuring the area is performing at the level it
should be, meeting all goals, and the quality of results being reported
are at the appropriate level meeting all necessary Quality Control (QC)
guidelines. 

Troubleshoot any problems with assays, work with their team to resolve
any professional conflicts, track all employee hours, and schedule
employee's time, and will  work with upper management to ensure all
policies and procedures are carried out, and that their unit meets all
necessary regulatory guidelines.

Knowledge, Skills, and Experience

1.	Ability to direct others as well as take direction from others.
2.	Ability to motivate and be persuasive.
3.	Routine histology including specimen processing, embedding,
microtomy, staining, and immunohistochemistry.
4.	May adapt procedures, processes, tools, equipment and techniques
to meet the more complex requirements of the position.
5.	Applies basic skills and may develop advanced skills using tools
and equipment appropriate for the position or specialization.
6.	Detail oriented, maintain a high level of person accountability,
and have sound problem solving skills.
7.	Ability to thrive in a team and service oriented environment and
must maintain a professional demeanor in interactions with physicians
and lab personnel.
8.	Demonstrated skills in technical data interpretation and ability
to troubleshoot.
9.	Demonstrated ability to take and follow direction and to know
who the resources are for their direction.
10.	Understanding of the equipment used daily; including, but not
limited to, microtome, stainer/cover slipper, slide labeler.
11.	Proficient in Microsoft Office Suite, specifically Word, Excel,
Outlook, and general working knowledge of Internet for business use.
12.	Ability to multi-task and work in a fast-past, high-volume,
high-stress deadline driven environment.
13.	Drive for Results (Service, Quality, and Continuous Improvement)
- Ensure procedures and processes are in place that lead to delivery of
quality results and continually reassess their effectiveness to achieve
continuous improvement.
14.	Communication - Proficient verbal and written communication
skills. Willingness to share and receive information and ideas from all
levels of the organization in order to achieve the desired results.
15.	Teamwork - Commitment to the successful achievement of team and
organizational goals through a desire to participate with and help other
members of the team.
16.	Customer Service Focus - Demonstrate a focus on listening to and
understanding client/customer needs and then delighting the
client/customer by exceeding service and quality expectations.

Please contact Michelle Hardin at mhardin@carisls.com for additional
details.  Thanks!

 

 

Michelle Hardin

Corporate Recruiter

Caris Life Sciences

502-418-9447

www.carisls.com

 

From brian <@t> prometheushealthcare.com  Thu Nov 24 07:39:51 2011
From: brian <@t> prometheushealthcare.com (Brian-Prometheus)
Date: Thu Nov 24 07:39:58 2011
Subject: [Histonet] Histology Manager Opening in NY
Message-ID: <003901ccaaae$8adef240$a09cd6c0$@com>

Top private pathology lab in NY searching for an experienced Histology
Manager for a day shift position.  

 

Position offers extremely competitive salary, free medical insurance and an
excellent 401k plan.  Great opportunity to become part of a rapidly growing
organization. 

 


Job Summary: 

Responsible for managing all functions of all Histology departments
including grossing, cyto prep, and IHC.  

Oversees daily operations of the departments, interacting and available to
staff and pathologists at all times. 

Coordinates staff orientation, training, competency assessment, performance
evaluation and scheduling. 

Develops and implements department policies, procedures, and quality control
programs. 

Ensures compliance with CLIA, State and CAP standards/ regulations. 

Promotes excellent customer satisfaction and patient safety.



Requirements:

Strong communication and interpersonal skills.

Bachelors degree required

Must have NY state license

At least 5 years management experience.  

Reference lab background is preferred.






 

 

 

Please contact me today for immediate consideration!

 

 

Brian Feldman

Principal

Prometheus Healthcare 

Office 301-693-9057

Fax 301-368-2478

 
 brian@prometheushealthcare.com

  www.prometheushealthcare.com

*** Stay up to date on the newest positions and healthcare trends nationwide
on Twitter!***

   http://twitter.com/PrometheusBlog

 

 

 

From eminoztas <@t> gata.edu.tr  Thu Nov 24 08:49:30 2011
From: eminoztas <@t> gata.edu.tr (=?utf-8?Q?Emin_=C3=96zta=C5=9F?=)
Date: Thu Nov 24 08:44:12 2011
Subject: [Histonet] demonstration of asbestos by means of electron
	microscopy
In-Reply-To: <1321972109.22669.YahooMailClassic@web65712.mail.ac4.yahoo.com>
Message-ID: <595608805.1849.1322146170910.JavaMail.root@zimbra.gata.edu.tr>

We showed aspestos fibers with EM by using a formvar coated 200 mesh grids. 1 micro liter sample solution was put on the surface of the formwar coated grid. The grid was dried at room temperature and directly searched with EM. 


----- Orijinal Mesaj -----
Kimden: "Rene J Buesa" 
Kime: histonet@lists.utsouthwestern.edu, "Yolanda Davies" 
G?nderilenler: 22 Kas?m Sal? 2011 16:28:29
Konu: Re: [Histonet] demonstration of asbestos by means of electron	microscopy

Many years ago asbestos fibers were demonstrated in hypochlorite digested tissues. The remaining material was filtered through Milllipore filter, washed and the fibers observed with polarized light.
The Perl's method will stain no the asbestos fibers (that cannot be stained) but a reactive capsule the tissues secrete around the fiber, so it is an indirect method based in: if there is a tissue reaction to asbestos, they should have been present at a certain moment.
As to its demonstration with EM I do not know but I think it will be very difficult to infiltrate?the fibers in Epoxi resin to be cut. If you are able to do that: how are you going to identify an extremely thin section of asbestos?
I think that you should either try the tissue digestion or give another try at the Perl reaction.
Ren? J.

--- On Tue, 11/22/11, Yolanda Davies  wrote:


From: Yolanda Davies 
Subject: [Histonet] demonstration of asbestos by means of electron microscopy
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, November 22, 2011, 4:14 AM


Dear all

I am a histotechnologist in forensics, Cape Town, South Africa.
I received a request to show asbestos in lung tissue where there is
definite interstitial fibrosis, but the presence of asbestos is not
clear.

Is it possible to reveal asbestos by means of electron microscopy?

Usually asbestos is demonstrated using the Perl's Prussian blue
technique, but most times they are elusive.
Could it be because of the sampling site or simply the nature of the
asbestos?

Thank you in advance

Yolanda Davies
Department of Forensic Medicine and Toxicology
Falmouth building
Anzio Road
Observatory
Cape Town 
South Africa




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Gulhane Military Medical Academy 
Department of Medical Histology and Embryology 
Ankara, 06018, Turkey 
eminoztas@gata.edu.tr 
+90 312 3043536 
+90 532 5215952 

From rjbuesa <@t> yahoo.com  Thu Nov 24 09:05:46 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Nov 24 09:05:53 2011
Subject: [Histonet] Question about Leica TP1020 and its tissue basket
	holders
In-Reply-To: 
Message-ID: <1322147146.19887.YahooMailClassic@web65704.mail.ac4.yahoo.com>

After?repeating so many times the same sequence anybody can get confused, either you or your supervisor.
Forget about verbal/personal instructions and follow the manual. It was developed to obtain a perfect sequence always.
Ren? J.

--- On Wed, 11/23/11, Jenny Vega  wrote:


From: Jenny Vega 
Subject: [Histonet] Question about Leica TP1020 and its tissue basket holders
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, November 23, 2011, 5:54 PM


Hello everyone. In my laboratory we have an old version of the Leica TP1020
tissue processor. My question is this. My supervisor told me that after the
machine finished processing the tissues on the next day, the tissue basket
was going to be in the last container which is going to be the second
container with paraffin wax. She told me that after removing the tissue
basket from the machine I had to rotate the tissue basket holder to it's
first station by pushing the the 'circle arrow' in the control panel which
is the first formalin container. In this machine the tissue basket holder
of both of the formalin containers are gray and the rest are black. (not
sure ALL of them are black but I know that the ones that are for the
paraffin containers are black).

I want to know if this is correct because when I go to the instruction
manuals and I go the "Removing the specimens" chapter it says


? Lift the carousel.
? Allow for the tissue basket to drain in that
position.
? Lift the tissue basket slightly with your hand
and pull it out of the basket holder in a horizontal
movement.
? Lower the carousel.

I got this manual online and its for the most recent Leica TP1020 machine
but it is still the same model. It doesnt says to rotate the tissue basket
holder to it's first station

When I put the tissue basket with new tissues I placed it in the first
station which is the first container of formalin, but what I don't remember
if? today I rotated the gray lid so it could be on top of the formalin
container. I think I did it in the morning but now I have my doubts. I have
always followed my supervisors instructions, what she told me makes sense
but I want to be sure if the processing cycle is going to be interrupted or
steps are going to be skipped if I don't return the "gray" tissue basket
holders to the formalin containers, and just leave it like the machine left
it after it finished the processing cycle. If you still placed the tissue
basket in the first formalin container would you still have issues or is
the machine going to follow the steps in order?


Thanks. I hope I do not sound confusing.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From Ken_Marissael <@t> vwr.com  Thu Nov 24 13:03:02 2011
From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com)
Date: Thu Nov 24 13:03:08 2011
Subject: [Histonet] Ken Marissael is out of the office
Message-ID: 


I will be out of the office starting  11/24/2011 and will not return until
11/30/2011.

I will be away on 11/24 and return 11/30. I will be out of the country, and
therefore not have e-mail or  cell phone access. While I am away, please
contact VWR Healthcare Customer Service at 877-881-1192.


From ricardosal84 <@t> aol.com  Thu Nov 24 15:34:44 2011
From: ricardosal84 <@t> aol.com (ricardosal84@aol.com)
Date: Thu Nov 24 15:34:49 2011
Subject: [Histonet] remove from list
Message-ID: <8CE790804693227-7B8-2E754@web-mmc-m08.sysops.aol.com>

Please unsubscribe me .


Thank you
From Steven.Weston <@t> utas.edu.au  Thu Nov 24 16:22:46 2011
From: Steven.Weston <@t> utas.edu.au (Steven Weston)
Date: Thu Nov 24 16:23:07 2011
Subject: [Histonet] re coverslippers
Message-ID: 

Hi everyone,
Just considering my options for the purchase of a new cover slipper and wanted some feedback on + and ? of different types.
I am mostly considering the new Dako one but would be interested to hear about peoples experience with the leica and others.
regards
Steve Weston
Lab Manager
Centre of Research Excellence for Chronic Respiratory Disease.
Menzies Research Institute
0408990859
62264871


From flourish.jf <@t> gmail.com  Fri Nov 25 01:55:13 2011
From: flourish.jf <@t> gmail.com (Janice Fuller)
Date: Fri Nov 25 01:55:17 2011
Subject: [Histonet] Survey
Message-ID: 

Please help me with my current assignment-

Our institution is reviewing grossing standards of small biopsies (GI/GU)
with the intent of formulating the best practice for error reduction
(floaters, tissue compression and loss of diagnostic use).  We are hoping
to get national feedback from other institutions on the following areas of
processing:

   1. Are forceps cleaned and/or changed between individual cases or parts
   of cases from the same patient while grossing?
   2. Are forceps cleaned and/or changed between individual cases or parts
   of cases from the same patient while embedding?
   3. Does the use of paper towel constitute a clean work surface? (i.e.
   one paper towel per case/part)
   4. Do you use forceps with teeth to gross/embed pieces that do not
   require sectioning?

Thank you, in advance, for your input.


-- 
Janice Fuller
From Nancy_Schmitt <@t> pa-ucl.com  Fri Nov 25 12:44:35 2011
From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt)
Date: Fri Nov 25 12:44:39 2011
Subject: [Histonet] Arginase 1
Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C367B9D0E@PEITHA.wad.pa-ucl.com>

Is anyone using Arginase 1 on the Bond system?  Where are you purchasing this from?  Is it staining well?

Thanks
Nancy



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is for the use of only the intended recipient(s) even if addressed
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that you have received it in error and then delete it along with any
attachments. Thank you.



From turkekul <@t> gmail.com  Fri Nov 25 13:20:24 2011
From: turkekul <@t> gmail.com (Mesruh Turkekul)
Date: Fri Nov 25 13:20:37 2011
Subject: [Histonet] Coverslipper
In-Reply-To: <4ecfd7c5.02ee960a.2056.276dSMTPIN_ADDED@mx.google.com>
References: <4ecfd7c5.02ee960a.2056.276dSMTPIN_ADDED@mx.google.com>
Message-ID: <81EA13BB-1187-4F04-A2CF-F74F4EFE2A54@gmail.com>

I use Leica cover slipper it is great, never gives problem

Mesruh

Sent from my iPad

On Nov 25, 2011, at 1:00 PM, histonet-request@lists.utsouthwestern.edu wrote:

> Send Histonet mailing list submissions to
>    histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>    http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>    histonet-request@lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
>    histonet-owner@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. Ken Marissael is out of the office (Ken_Marissael@vwr.com)
>   2. remove from list (ricardosal84@aol.com)
>   3. re coverslippers (Steven Weston)
>   4. Survey (Janice Fuller)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Thu, 24 Nov 2011 14:03:02 -0500
> From: Ken_Marissael@vwr.com
> Subject: [Histonet] Ken Marissael is out of the office
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
>    
> Content-Type: text/plain; charset=US-ASCII
> 
> 
> I will be out of the office starting  11/24/2011 and will not return until
> 11/30/2011.
> 
> I will be away on 11/24 and return 11/30. I will be out of the country, and
> therefore not have e-mail or  cell phone access. While I am away, please
> contact VWR Healthcare Customer Service at 877-881-1192.
> 
> 
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Thu, 24 Nov 2011 16:34:44 -0500 (EST)
> From: ricardosal84@aol.com
> Subject: [Histonet] remove from list
> To: Histonet@lists.utsouthwestern.edu
> Message-ID: <8CE790804693227-7B8-2E754@web-mmc-m08.sysops.aol.com>
> Content-Type: text/plain; charset="us-ascii"
> 
> Please unsubscribe me .
> 
> 
> Thank you
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Thu, 24 Nov 2011 22:22:46 +0000
> From: Steven Weston 
> Subject: [Histonet] re coverslippers
> To: "histonet@lists.utsouthwestern.edu"
>    
> Message-ID: 
> Content-Type: text/plain; charset=Windows-1252
> 
> Hi everyone,
> Just considering my options for the purchase of a new cover slipper and wanted some feedback on + and ? of different types.
> I am mostly considering the new Dako one but would be interested to hear about peoples experience with the leica and others.
> regards
> Steve Weston
> Lab Manager
> Centre of Research Excellence for Chronic Respiratory Disease.
> Menzies Research Institute
> 0408990859
> 62264871
> 
> 
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Fri, 25 Nov 2011 02:55:13 -0500
> From: Janice Fuller 
> Subject: [Histonet] Survey
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
>    
> Content-Type: text/plain; charset=ISO-8859-1
> 
> Please help me with my current assignment-
> 
> Our institution is reviewing grossing standards of small biopsies (GI/GU)
> with the intent of formulating the best practice for error reduction
> (floaters, tissue compression and loss of diagnostic use).  We are hoping
> to get national feedback from other institutions on the following areas of
> processing:
> 
>   1. Are forceps cleaned and/or changed between individual cases or parts
>   of cases from the same patient while grossing?
>   2. Are forceps cleaned and/or changed between individual cases or parts
>   of cases from the same patient while embedding?
>   3. Does the use of paper towel constitute a clean work surface? (i.e.
>   one paper towel per case/part)
>   4. Do you use forceps with teeth to gross/embed pieces that do not
>   require sectioning?
> 
> Thank you, in advance, for your input.
> 
> 
> -- 
> Janice Fuller
> 
> 
> ------------------------------
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> End of Histonet Digest, Vol 96, Issue 37
> ****************************************

From Jennifer.Bull <@t> northwestpathology.com  Fri Nov 25 13:26:36 2011
From: Jennifer.Bull <@t> northwestpathology.com (Bull, Jennifer L.)
Date: Fri Nov 25 13:26:40 2011
Subject: [Histonet] Spirochete Control Tissue
Message-ID: <85760CECEC18444BB95F26D5E88DAEAA22EE40BBFE@hinet2.hinet.org>

Does anyone out there have any Spirochete Control tissue they would be willing to trade or sell?

Thanks!
Jenny




mailgate.hinet.org made the following annotations
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From SandraDiaz <@t> texashealth.org  Fri Nov 25 17:38:27 2011
From: SandraDiaz <@t> texashealth.org (Diaz, Sandra)
Date: Fri Nov 25 17:38:41 2011
Subject: [Histonet] GLUT-1 antibody
Message-ID: 

We recently purchased Cell Marque's Glut-1 antibody and it is not staining correctly for us.  Does anyone have a working protocol for the Benchmark Ultra?  Or is the antibody from another company work anyone?

Sandra Diaz H.T.
IHC Department, HMFW


The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law.  If you are not the intended recipient, you are prohibited from copying, distributing, or using the information.  Please contact the sender immediately by return e-mail and delete the original message from your system.
From hodges420 <@t> msn.com  Fri Nov 25 20:04:03 2011
From: hodges420 <@t> msn.com (MARY T HODGES )
Date: Fri Nov 25 20:04:07 2011
Subject: [Histonet] is there a way to recover ruined tissues
Message-ID: 

Wrong all most complete recovery can be made with phenol 95 and water over 24 hours
Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: Rene J Buesa 
Date: Tue, 15 Nov 2011 21:14:20 
To: ; 
Subject: Re: [Histonet] is there a way to recover ruined tissues

Realy recovered? No!
 Ren? J.
 
 --- On Tue, 11/15/11, Patsy Ruegg  wrote:
 
 
 From: Patsy Ruegg 
 Subject: [Histonet] is there a way to recover ruined tissues
 To: "'histo net'" 
 Date: Tuesday, November 15, 2011, 3:26 PM
 
 
 My tissue processor was loaded and although the technician thought they
 started it, the next morning the tissues were found dry in the retort with
 the processor not started.? Another technician just started the machine so
 the tissues got processed as they would have been had it started the day
 before.? The tissues look terrible, they shrank so much that the pieces
 started coming out of the cassettes even though they were plenty large
 enough in the first place.? The tissues were found to be very small and hard
 but were embedded, sectioned and stained anyway (I was not informed about
 this until I started questioning why the tissues looked so bad).
 
 
 
 Of course the investigator is very upset that their tissues are ruined.
 Does anyone know if there is any way to try and recovery these tissues?
 They were mouse lungs and livers.? The livers are not as bad as the lungs,
 the lungs were inflated and they look compressed and unrecognizable as lung
 tissue.? If the investigator will ever speak to me again I am going to ask
 for the blocks back and try melting them down and perhaps try to rehydrate
 them somehow for reprocessing.
 
 
 
 Regards,
 
 
 
 Patsy
 
 
 
 Patsy Ruegg, HT(ASCP)QIHC
 
 IHCtech
 
 12635 Montview Blvd. Ste.215
 
 Aurora, CO 80045
 
 720-859-4060
 
 fax 720-859-4110
 
 www.ihctech.net   
 
 www.ihcrg.org  
 
 
 
 _______________________________________________
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 _______________________________________________
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From vagishar <@t> gmail.com  Fri Nov 25 22:02:07 2011
From: vagishar <@t> gmail.com (Vagisha R)
Date: Fri Nov 25 22:02:12 2011
Subject: [Histonet] Cutting mouse skin sections
Message-ID: 

Hi,

I've been trying to do hair follicle analysis in mouse skin sections for
the past couple of months.I usually fix the skin in Neutral Buffer
Formaline (NBF) for 24-48 hours and then send it to our histology
department to be embedded in paraffin blocks. However,i'm repeatedly
getting cross sections and broken hair follicles in my skin sections.Ive
tried embedding skin supported on paper and cutting the blocks at various
angles but it didn't make too much of a difference.If anything the stupid
paper is ruining the cutting blades!
Ive also tried cryosectioning snap frozen skin samples but that isn't
working out very well either.Does anyone have any clue at all why this is
happening and what i could possibly do to improve the section quality?

Thanks
Vagisha
From jkiernan <@t> uwo.ca  Fri Nov 25 23:03:37 2011
From: jkiernan <@t> uwo.ca (John Kiernan)
Date: Fri Nov 25 23:03:40 2011
Subject: [Histonet] Cutting mouse skin sections
In-Reply-To: <7660976714cd.4ed0730a@uwo.ca>
References: 
	<76308d22213f.4ed06f35@uwo.ca> <7550851c3028.4ed06f71@uwo.ca>
	<7610edb43d35.4ed06faf@uwo.ca> <761090b71244.4ed06fec@uwo.ca>
	<7610afff481c.4ed0702a@uwo.ca> <75e0a2527e95.4ed07067@uwo.ca>
	<76509cf25d1a.4ed070a4@uwo.ca> <7410b2c22d1.4ed070e2@uwo.ca>
	<76a0996a97c.4ed0711f@uwo.ca> <7660b87ed47.4ed0715d@uwo.ca>
	<75e0dba144fc.4ed0719a@uwo.ca> <7540eeb977a9.4ed071d7@uwo.ca>
	<7410a50369b6.4ed07215@uwo.ca> <7410f4053bad.4ed07252@uwo.ca>
	<7620aa666790.4ed072cc@uwo.ca> <7660976714cd.4ed0730a@uwo.ca>
Message-ID: <7660ee77150.4ed02cd9@uwo.ca>

You need the advice of an experienced technician. Probably you will be advised to remove the paper after fixation and before trying to obtain either paraffin or cryostat sections.  You have a histology department that processes your fixed tissues.  Ask  their technicians for advice. 
 
Another word of advice from me: Say who and where you are. Do not hide behind a gmail address and expect  to get good, free answers to your questions on Histonet or any other forum. 
 
John Kiernan 
Anatomy, UWO 
London, Canada 
= = = 
On 25/11/11, Vagisha R  wrote:

> 
> Hi,
> 
> I've been trying to do hair follicle analysis in mouse skin sections for
> the past couple of months.I usually fix the skin in Neutral Buffer
> Formaline (NBF) for 24-48 hours and then send it to our histology
> department to be embedded in paraffin blocks. However,i'm repeatedly
> getting cross sections and broken hair follicles in my skin sections.Ive
> tried embedding skin supported on paper and cutting the blocks at various
> angles but it didn't make too much of a difference.If anything the stupid
> paper is ruining the cutting blades!
> Ive also tried cryosectioning snap frozen skin samples but that isn't
> working out very well either.Does anyone have any clue at all why this is
> happening and what i could possibly do to improve the section quality?
> 
> Thanks
> Vagisha
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 

 
From crochieresteve <@t> aol.com  Fri Nov 25 23:26:40 2011
From: crochieresteve <@t> aol.com (Steven)
Date: Fri Nov 25 23:27:26 2011
Subject: [Histonet] (no subject)
Message-ID: <8CE7A131C3239F6-19F8-77D77@webmail-m160.sysops.aol.com>

http://bambigalore.com/wp-content/themes/dark-ritual-10/mynewgood.html

From pruegg <@t> ihctech.net  Sat Nov 26 11:34:08 2011
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Sat Nov 26 11:34:13 2011
Subject: [Histonet] is there a way to recover ruined tissues
In-Reply-To: 
References: 
Message-ID: <01411EB20B9D48738915EFB5CCB9A42B@prueggihctechlt>

Please elaborate on this method.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: MARY T HODGES [mailto:hodges420@msn.com] 
Sent: Friday, November 25, 2011 7:04 PM
To: Rene J Buesa ; histonet@lists.utsouthwestern.edu ; pruegg@ihctech.net 
Subject: Re: [Histonet] is there a way to recover ruined tissues

Wrong all most complete recovery can be made with phenol 95 and water over
24 hours
Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: Rene J Buesa 
Date: Tue, 15 Nov 2011 21:14:20 
To: ; 
Subject: Re: [Histonet] is there a way to recover ruined tissues

Realy recovered? No!
 Ren? J.
 
 --- On Tue, 11/15/11, Patsy Ruegg  wrote:
 
 
 From: Patsy Ruegg 
 Subject: [Histonet] is there a way to recover ruined tissues
 To: "'histo net'" 
 Date: Tuesday, November 15, 2011, 3:26 PM
 
 
 My tissue processor was loaded and although the technician thought they
 started it, the next morning the tissues were found dry in the retort with
 the processor not started.? Another technician just started the machine so
 the tissues got processed as they would have been had it started the day
 before.? The tissues look terrible, they shrank so much that the pieces
 started coming out of the cassettes even though they were plenty large
 enough in the first place.? The tissues were found to be very small and
hard
 but were embedded, sectioned and stained anyway (I was not informed about
 this until I started questioning why the tissues looked so bad).
 
 
 
 Of course the investigator is very upset that their tissues are ruined.
 Does anyone know if there is any way to try and recovery these tissues?
 They were mouse lungs and livers.? The livers are not as bad as the lungs,
 the lungs were inflated and they look compressed and unrecognizable as lung
 tissue.? If the investigator will ever speak to me again I am going to ask
 for the blocks back and try melting them down and perhaps try to rehydrate
 them somehow for reprocessing.
 
 
 
 Regards,
 
 
 
 Patsy
 
 
 
 Patsy Ruegg, HT(ASCP)QIHC
 
 IHCtech
 
 12635 Montview Blvd. Ste.215
 
 Aurora, CO 80045
 
 720-859-4060
 
 fax 720-859-4110
 
 www.ihctech.net   
 
 www.ihcrg.org  
 
 
 
 _______________________________________________
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 _______________________________________________
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From pruegg <@t> ihctech.net  Sun Nov 27 09:55:58 2011
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Sun Nov 27 09:56:03 2011
Subject: [Histonet] dako autostainer labeler
Message-ID: 

Greetings,

 

I am looking for another Seymour labeler like the one used on the Dako
Autostainer, does anyone have one sitting around not being used I could
purchase from you?  It needs to have the software disc so I can install it
on a computer.

 

Regards,

 

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC

IHCtech

12635 Montview Blvd. Ste.215

Aurora, CO 80045

720-859-4060

fax 720-859-4110

www.ihctech.net 

www.ihcrg.org

 

From nto <@t> stowers.org  Mon Nov 28 07:01:06 2011
From: nto <@t> stowers.org (Thomas, Nancy)
Date: Mon Nov 28 07:01:19 2011
Subject: [Histonet] Question about Leica TP1020 and its tissue basket
	holders
In-Reply-To: 
References: 
Message-ID: <2C40E43D1F7A56408C4463FD245DDDF99398FF32@EXCHMB-02.stowers-institute.org>

When you rotate the tissue basket holder to the 1st formalin, it prevents someone from forgetting to do that when they start the processor later.  If it doesn't get rotated, the unprocessed tissues will drop right down into paraffin.  It just must be rotated either at the end of a run (which is what we do to make sure it gets done) or at the beginning of a run.  It will not change the outcome of the next run.  Always inspect the location of the "gray" basket before starting a run.

Nancy Thomas 
Stowers Institute for Medical Research
Kansas City, MO

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega
Sent: Wednesday, November 23, 2011 4:55 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question about Leica TP1020 and its tissue basket holders

Hello everyone. In my laboratory we have an old version of the Leica TP1020 tissue processor. My question is this. My supervisor told me that after the machine finished processing the tissues on the next day, the tissue basket was going to be in the last container which is going to be the second container with paraffin wax. She told me that after removing the tissue basket from the machine I had to rotate the tissue basket holder to it's first station by pushing the the 'circle arrow' in the control panel which is the first formalin container. In this machine the tissue basket holder of both of the formalin containers are gray and the rest are black. (not sure ALL of them are black but I know that the ones that are for the paraffin containers are black).

I want to know if this is correct because when I go to the instruction manuals and I go the "Removing the specimens" chapter it says


* Lift the carousel.
* Allow for the tissue basket to drain in that position.
* Lift the tissue basket slightly with your hand and pull it out of the basket holder in a horizontal movement.
* Lower the carousel.

I got this manual online and its for the most recent Leica TP1020 machine but it is still the same model. It doesnt says to rotate the tissue basket holder to it's first station

When I put the tissue basket with new tissues I placed it in the first station which is the first container of formalin, but what I don't remember if  today I rotated the gray lid so it could be on top of the formalin container. I think I did it in the morning but now I have my doubts. I have always followed my supervisors instructions, what she told me makes sense but I want to be sure if the processing cycle is going to be interrupted or steps are going to be skipped if I don't return the "gray" tissue basket holders to the formalin containers, and just leave it like the machine left it after it finished the processing cycle. If you still placed the tissue basket in the first formalin container would you still have issues or is the machine going to follow the steps in order?


Thanks. I hope I do not sound confusing.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From rmhickey88 <@t> gmail.com  Mon Nov 28 08:32:25 2011
From: rmhickey88 <@t> gmail.com (Ryan Hickey)
Date: Mon Nov 28 08:32:32 2011
Subject: [Histonet] Graduate Seeks Advice Concerning Histology Careers
Message-ID: 

 Histonet Listserv Members,

I am a recent graduate with a B.S. in Biology and a minor in Chemistry. I
have completed over 80 hours of laboratory training in histology by means
of a Biological Microtechniques course at my college.

During that time, I was familiarized with the basics of histochemistry,
specimen fixation, paraffin embedding, microtome use/serial sectioning,
complex staining, mounting, and digital image optimization. I have amassed
a modest portfolio of my work with several animal tissues as well as
foliose lichens.

I am looking for an opportunity to further develop my knowledge and
practice of histological techniques so that I may meet the eligibility
requirements for HT (ASCP) certification and begin a career in this
extraordinary field. As of now, I have no geographical preference. Is there
any way to develop experience (paid if possible) in a laboratory setting
for someone of my qualifications?

I am looking forward to the possibility of showcasing some of my work,
learning more about any possible opportunities, and developing a course of
action to get involved in a career in histology. Thank you for your
consideration, and all the hospitality and advice Histonet has provided
thus far.


Yours Sincerely,
Ryan M. Hickey
From mhale <@t> carisls.com  Mon Nov 28 08:48:20 2011
From: mhale <@t> carisls.com (Hale, Meredith)
Date: Mon Nov 28 08:48:33 2011
Subject: [Histonet] Louisiana Position 
Message-ID: <6F33D8418806044682A391273399860F0AB77375@s-irv-ex301.PathologyPartners.intranet>

NO recruiters 

 

 

Great opportunity for a Histotechnician in a brand new laboratory!
Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice
located in Lafayette, Louisiana, is looking for a certified HT or HTL to
run their newly constructed laboratory. Candidate must be ASCP certified
and CLIA certified to perform gross dissection, prior supervisory
experience preferred. Responsibilities would include the following:
Creation and maintenance of policies and procedures to CLIA standards,
leading lab through CLIA inspection, maintenance and quality control for
equipment, and routine histology duties. This is a part-time position
that offers a competitive rate and flexible hours.  Interested
applicants should contact Meredith Hale;  phone 214-596-2219 or through
email mhale@carisls.com
  

 

 

Meredith Hale HT  (ASCP)cm

Operations Liaision Director and Education Coordinator 

 

Caris Diagnostics 

Part of Miraca Holdings Inc. 

6655 North MacArthur Blvd. 

Irving , Texas 75039

Office: 214-596-2219

Cell: 469-648-8253

 

 

 

 

From mhale <@t> carisls.com  Mon Nov 28 08:50:38 2011
From: mhale <@t> carisls.com (Hale, Meredith)
Date: Mon Nov 28 08:50:52 2011
Subject: [Histonet] AZ position
Message-ID: <6F33D8418806044682A391273399860F0AB77383@s-irv-ex301.PathologyPartners.intranet>

No Recruiters 

Great opportunity for a Histotechnician in Scottsdale , AZ . Colon and
Digestive Health Specialists  is looking for a certified HT or HTL to
run their  laboratory. Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred. Responsibilities would include the following: Creation and
maintenance of policies and procedures to CLIA standards, leading lab
through CLIA inspection, maintenance and quality control for equipment,
and routine histology duties. This is a part-time position that offers a
competitive rate and flexible hours up to 25 hours a week .  Interested
applicants should contact Meredith Hale;  phone 214-596-2219 or through
email mhale@carisls.com
  

 

 

 

Meredith Hale HT  (ASCP)cm

Operations Liaision Director and Education Coordinator 

 

Caris Diagnostics 

Part of Miraca Holdings Inc. 

6655 North MacArthur Blvd. 

Irving , Texas 75039

Office: 214-596-2219

Cell: 469-648-8253

 

 

 

 

From Rcartun <@t> harthosp.org  Mon Nov 28 10:10:20 2011
From: Rcartun <@t> harthosp.org (Richard Cartun)
Date: Mon Nov 28 10:10:36 2011
Subject: [Histonet] Pathologists' Assistants - Survey
Message-ID: <4ED36C1B.7400.0077.1@harthosp.org>

I need your help.  This question is for Anatomic Pathology Laboratories located in hospitals where the pathologists are in private practice.

Do you employ Pathologists' Assistants in your Anatomic Pathology Laboratory?  If so, are they employed by the hospital or do the pathologists employ them?

Thank you for your time.

Richard  

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



From Richard.Breckenridge <@t> uth.tmc.edu  Mon Nov 28 10:16:14 2011
From: Richard.Breckenridge <@t> uth.tmc.edu (Breckenridge, Richard A)
Date: Mon Nov 28 10:16:19 2011
Subject: [Histonet] IgG antibodies for Renal Path
Message-ID: <3FE6D14CF4B4174AA4783A50F58CD30D0F1F6D361E@UTHCMS3.uthouston.edu>

Hi All,
I need to order IgG 1,2,3, and 4 for fluorescence. Any suggestions?

Rick

Richard A. Breckenridge, HT(ASCP)
Histology Laboratory Manager
University of Texas Health Science Center at Houston
Medical School Building 2.234
ph. 713-500-6792  fax 713-500-0733
richard.breckenridge@uth.tmc.edu

From Melissa.Kuhnla <@t> chsli.org  Mon Nov 28 11:31:03 2011
From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa)
Date: Mon Nov 28 11:31:11 2011
Subject: [Histonet] Pathologists' Assistants - Survey
In-Reply-To: <4ED36C1B.7400.0077.1@harthosp.org>
References: <4ED36C1B.7400.0077.1@harthosp.org>
Message-ID: 

Hi Dr. Cartun,
I am currently working in a hospital setting as you described.  We have
two PAs working here that are employed by the hospital and not the
pathology group.
Melissa

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Monday, November 28, 2011 11:10 AM
To: Histonet
Subject: [Histonet] Pathologists' Assistants - Survey

I need your help.  This question is for Anatomic Pathology Laboratories
located in hospitals where the pathologists are in private practice.

Do you employ Pathologists' Assistants in your Anatomic Pathology
Laboratory?  If so, are they employed by the hospital or do the
pathologists employ them?

Thank you for your time.

Richard  

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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From dmccaig <@t> ckha.on.ca  Mon Nov 28 12:03:46 2011
From: dmccaig <@t> ckha.on.ca (Diana McCaig)
Date: Mon Nov 28 12:05:02 2011
Subject: [Histonet] PowerPath Turn Around Time Report
Message-ID: 

Hi

Does anyone using PowerPath have a turn around time report?

 

Diana McCaig

Histology Lab

Chatham Kent Health Alliance

80 Grand Avenue West

Chatham. Ontario

N7L 1B7

519-352-6401  (6604)

 

This email communication and any files transmitted with it may contain
confidential and or proprietary information and is provided for the use
of the intended recipient only. Any review, retransmission or
dissemination of this information by anyone other than the intended
recipient is prohibited. If you receive this email in error, please
contact the sender and delete this communication and any copies
immediately. Thank you. 

 

From Ronald.Houston <@t> nationwidechildrens.org  Mon Nov 28 12:52:34 2011
From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald)
Date: Mon Nov 28 12:52:43 2011
Subject: [Histonet] calmodulin
Message-ID: 

Anyone had success getting Calmodulin up and running? I'm running in to some difficulties with an Abcam antibody one of our researchers gave me.

Thanks for any assistance
Ronnie


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster



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From esarricks <@t> gmail.com  Mon Nov 28 12:54:42 2011
From: esarricks <@t> gmail.com (Erin Sarricks)
Date: Mon Nov 28 12:54:45 2011
Subject: [Histonet] Woelcke Myelin Stain
Message-ID: 

Hi Histonet-

I hope everyone had a great Thanksgiving!  I was wondering if anyone has
any experience with the Woelcke Myelin Stain.  A researcher is trying to
use it to identify damaged neurons in brain sections.  I have found one
article with a rough procedure, but any other help anyone has would be
great!  Thanks!

Erin Sarricks


Erin Sarricks, HT (ASCP)

Histology Team Leader

USAMRICD Comparative Pathology Branch

Office: Bldg E-3081 Room 178

E-mail: erin.p.sarricks@us.army.mil

Phone: 410-436-1967
From wbenton <@t> cua.md  Mon Nov 28 13:04:49 2011
From: wbenton <@t> cua.md (Walter Benton)
Date: Mon Nov 28 13:04:58 2011
Subject: [Histonet] Woelcke Myelin Stain
In-Reply-To: 
References: 
Message-ID: <0B8979A204680A42B93A52B486088CD9206C2044D5@CUAEXH1.GCU-MD.local>

http://www.rowleybio.com/pdfs/K-684%20Woelcke's%20Method%20for%20Myelin%20Sheath.pdf

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wbenton@cua.md
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks [esarricks@gmail.com]
Sent: Monday, November 28, 2011 1:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Woelcke Myelin Stain

Hi Histonet-

I hope everyone had a great Thanksgiving!  I was wondering if anyone has
any experience with the Woelcke Myelin Stain.  A researcher is trying to
use it to identify damaged neurons in brain sections.  I have found one
article with a rough procedure, but any other help anyone has would be
great!  Thanks!

Erin Sarricks


Erin Sarricks, HT (ASCP)

Histology Team Leader

USAMRICD Comparative Pathology Branch

Office: Bldg E-3081 Room 178

E-mail: erin.p.sarricks@us.army.mil

Phone: 410-436-1967
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CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law.  If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy.

From amber.mckenzie <@t> gastrodocs.net  Mon Nov 28 14:00:03 2011
From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie)
Date: Mon Nov 28 13:58:56 2011
Subject: [Histonet] validation
In-Reply-To: <0B8979A204680A42B93A52B486088CD9206C2044D5@CUAEXH1.GCU-MD.local>
References: 
	<0B8979A204680A42B93A52B486088CD9206C2044D5@CUAEXH1.GCU-MD.local>
Message-ID: <5A33C952BB67F4468AF1F36D739212BC0642BE@JERRY.Gia.com>

Do you have to validate the neg mouse and rabbit?


From Wanda.Smith <@t> HCAhealthcare.com  Mon Nov 28 14:12:28 2011
From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com)
Date: Mon Nov 28 14:12:36 2011
Subject: [Histonet] Pathologists' Assistants - Survey
In-Reply-To: <4ED36C1B.7400.0077.1@harthosp.org>
References: <4ED36C1B.7400.0077.1@harthosp.org>
Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA2733E69A6D@NADCWPMSGCMS03.hca.corpad.net>

Good Afternoon,
Our Pathologist group employs their PA.  The hospital furnishes the space and all the supplies.
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Monday, November 28, 2011 11:10 AM
To: Histonet
Subject: [Histonet] Pathologists' Assistants - Survey

I need your help.  This question is for Anatomic Pathology Laboratories located in hospitals where the pathologists are in private practice.

Do you employ Pathologists' Assistants in your Anatomic Pathology Laboratory?  If so, are they employed by the hospital or do the pathologists employ them?

Thank you for your time.

Richard  

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From rjbuesa <@t> yahoo.com  Mon Nov 28 15:22:21 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Mon Nov 28 15:22:25 2011
Subject: [Histonet] validation
In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC0642BE@JERRY.Gia.com>
Message-ID: <1322515341.90476.YahooMailClassic@web65704.mail.ac4.yahoo.com>

I have been thinking about your question and it seems to me that it is similar to proving a negative or a non existent situation or entity. Proving the non-existence of something is impossible the same as proving the existence of something non-existent.
On the other hand, you may have a protocol so "off" that you could?have a heavy background in a supposedly non reacting section, and you could try to adjust it to make sure that the "non-reactive" tissue should just be that, negative.
You could run your negative through the whole protocol and compare its "negativity" against a section that has NOT been run through your protocol, just hematoxylin stained, and see if both are equal.
Other than that I do not think "validating a negative" is worth much thought or effort.
I never did it, and I do not see the advantage of doing it, although I may be wrong (as in many an occasion).
Ren? J.

--- On Mon, 11/28/11, Amber McKenzie  wrote:


From: Amber McKenzie 
Subject: [Histonet] validation
To: "histonet@lists.utsouthwestern.edu" 
Date: Monday, November 28, 2011, 3:00 PM


Do you have to validate the neg mouse and rabbit?


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From JWeems <@t> sjha.org  Mon Nov 28 16:14:02 2011
From: JWeems <@t> sjha.org (Weems, Joyce)
Date: Mon Nov 28 16:14:10 2011
Subject: [Histonet] Pathologists' Assistants - Survey
In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA2733E69A6D@NADCWPMSGCMS03.hca.corpad.net>
References: <4ED36C1B.7400.0077.1@harthosp.org>
	<9E2D36CE2D7CBA4A94D9B22E8328A3BA2733E69A6D@NADCWPMSGCMS03.hca.corpad.net>
Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640845443A57@CHEXCMS10.one.ads.che.org>

Same for us.


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com
Sent: Monday, November 28, 2011 15:12
To: Rcartun@harthosp.org; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Pathologists' Assistants - Survey

Good Afternoon,
Our Pathologist group employs their PA.  The hospital furnishes the space and all the supplies.
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Monday, November 28, 2011 11:10 AM
To: Histonet
Subject: [Histonet] Pathologists' Assistants - Survey

I need your help.  This question is for Anatomic Pathology Laboratories located in hospitals where the pathologists are in private practice.

Do you employ Pathologists' Assistants in your Anatomic Pathology Laboratory?  If so, are they employed by the hospital or do the pathologists employ them?

Thank you for your time.

Richard  

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Confidentiality Notice:
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It may contain information that is privileged and 
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disclosure, or distribution is prohibited. If you are 
not the intended recipient, please delete this message, and 
reply to the sender regarding the error in a separate email. 
 


From gayle.callis <@t> bresnan.net  Mon Nov 28 17:38:49 2011
From: gayle.callis <@t> bresnan.net (gayle callis)
Date: Mon Nov 28 17:39:27 2011
Subject: [Histonet] Re: Woelcke"s Myelin Sheath method
Message-ID: <001d01ccae26$e2da4f50$a88eedf0$@bresnan.net>

You wrote:  

 

I hope everyone had a great Thanksgiving!  I was wondering if anyone has

any experience with the Woelcke Myelin Stain.  A researcher is trying to

use it to identify damaged neurons in brain sections.  I have found one

article with a rough procedure, but any other help anyone has would be

great!  Thanks!

 

Erin Sarricks

************************************************

 

Erin,  

 

If you contact me, I will personally send you the procedure.   It was called
Lee Luna Woelcke Myelin Sheath method in a publication I found.  I will scan
the method and send to you in pdf format IF you email me.  Pretty simple to
do but hopefully John Kiernan, a myelin staining expert will make
suggestions too.   He wrote a fantastic review of myelin staining about 6
years ago that is classic tutorial on the subject.   This was published in
Journal of Histotechnology, and can be accessed online by NSH members free
of charge.  If you go to Google Scholar, you can pick up this publication
Cereb. Cortex (1997) 7 (2): 166-177. doi: 10.1093/cercor/7.2.166   Or merely
cut and paste the whole DOI link into Google to get free pdf/full text
article.   

 

 

Gayle Callis

HTL/HT/MT(ASCP)

Bozeman MT 

 

From W.E.J.Hoekert <@t> olvg.nl  Tue Nov 29 02:49:19 2011
From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.)
Date: Tue Nov 29 02:49:27 2011
Subject: [Histonet] GLUT-1 antibody
References: 
Message-ID: <1190CB05C44B13409483514729C2FC3601F841F6@PAIT42.olvg.nl>

Hi Sandra,
 
I am using Neomarkers (Glut 1 Ab-1, polyclonal) on the Benchmark XT.
 
30' cc1
32' ab incubation
1:50
 
It works fine. 
 
 
Willem Hoekert
OLVG Amsterdam
The Netherlands
 
 
 
________________________________

Van: histonet-bounces@lists.utsouthwestern.edu namens Diaz, Sandra
Verzonden: za 26-11-2011 0:38
Aan: 'histonet@lists.utsouthwestern.edu'
Onderwerp: [Histonet] GLUT-1 antibody



We recently purchased Cell Marque's Glut-1 antibody and it is not staining correctly for us.  Does anyone have a working protocol for the Benchmark Ultra?  Or is the antibody from another company work anyone?

Sandra Diaz H.T.
IHC Department, HMFW


The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law.  If you are not the intended recipient, you are prohibited from copying, distributing, or using the information.  Please contact the sender immediately by return e-mail and delete the original message from your system._______________________________________________
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From sdysart <@t> mirnarx.com  Tue Nov 29 09:53:40 2011
From: sdysart <@t> mirnarx.com (Sarah Dysart)
Date: Tue Nov 29 09:53:54 2011
Subject: [Histonet] Ahh...
Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D500211ED@SN2PRD0702MB110.namprd07.prod.outlook.com>

So I have a bunch of samples I processed last night and looks like I am not going to be able to embed them today.  They are mouse samples, so smaller than others.  Would it be best just to pull them out of the processor (they are currently in hot paraffin) let them solidify at room temp, and then tomorrow just put them in the embedder to remelt?  Or should something else be done to preserve them.
Thanks

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

From JWeems <@t> sjha.org  Tue Nov 29 10:02:59 2011
From: JWeems <@t> sjha.org (Weems, Joyce)
Date: Tue Nov 29 10:03:09 2011
Subject: [Histonet] RE: Ahh...
In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D500211ED@SN2PRD0702MB110.namprd07.prod.outlook.com>
References: <8A70A9B2ECDD084DACFE6C59FCF86D500211ED@SN2PRD0702MB110.namprd07.prod.outlook.com>
Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640845443B46@CHEXCMS10.one.ads.che.org>

That is what I would do.  


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Tuesday, November 29, 2011 10:54
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ahh...

So I have a bunch of samples I processed last night and looks like I am not going to be able to embed them today.  They are mouse samples, so smaller than others.  Would it be best just to pull them out of the processor (they are currently in hot paraffin) let them solidify at room temp, and then tomorrow just put them in the embedder to remelt?  Or should something else be done to preserve them.
Thanks

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

_______________________________________________
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This e-mail, including any attachments is the 
property of Catholic Health East and is intended 
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It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
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not the intended recipient, please delete this message, and 
reply to the sender regarding the error in a separate email.


From SLB <@t> stowers.org  Tue Nov 29 10:12:17 2011
From: SLB <@t> stowers.org (Beckham, Sharon)
Date: Tue Nov 29 10:12:22 2011
Subject: [Histonet] RE: Ahh...
In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640845443B46@CHEXCMS10.one.ads.che.org>
References: <8A70A9B2ECDD084DACFE6C59FCF86D500211ED@SN2PRD0702MB110.namprd07.prod.outlook.com>
	<92AD9B20A6C38C4587A9FEBE3A30E1640845443B46@CHEXCMS10.one.ads.che.org>
Message-ID: <2C40E43D1F7A56408C4463FD245DDDF993A93EF9@EXCHMB-02.stowers-institute.org>

We do that a lot and have no problem at all.

Sharon Beckham
Head, Histotechnology
Stowers Institute for Medical Research
1000 E 50th St
Kansas City, MO  64110
816-926-4305
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Tuesday, November 29, 2011 10:03 AM
To: Sarah Dysart; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Ahh...

That is what I would do.  


Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Tuesday, November 29, 2011 10:54
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ahh...

So I have a bunch of samples I processed last night and looks like I am not going to be able to embed them today.  They are mouse samples, so smaller than others.  Would it be best just to pull them out of the processor (they are currently in hot paraffin) let them solidify at room temp, and then tomorrow just put them in the embedder to remelt?  Or should something else be done to preserve them.
Thanks

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

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From rjbuesa <@t> yahoo.com  Tue Nov 29 10:25:51 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Nov 29 10:25:55 2011
Subject: [Histonet] Ahh...
In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D500211ED@SN2PRD0702MB110.namprd07.prod.outlook.com>
Message-ID: <1322583951.90292.YahooMailClassic@web65708.mail.ac4.yahoo.com>

Yes, you can do that but the best way is NOT to take out the individual cassettes and let the solidify individually?at RT.
Prepare a container, fill it with melted paraffin and place all the cassettes inside. Then let that "gigantic-common" block to solidify. Melt it?completely before doing the embedding whenever you can.
Ren? J.

--- On Tue, 11/29/11, Sarah Dysart  wrote:


From: Sarah Dysart 
Subject: [Histonet] Ahh...
To: "histonet@lists.utsouthwestern.edu" 
Date: Tuesday, November 29, 2011, 10:53 AM


So I have a bunch of samples I processed last night and looks like I am not going to be able to embed them today.? They are mouse samples, so smaller than others.? Would it be best just to pull them out of the processor (they are currently in hot paraffin) let them solidify at room temp, and then tomorrow just put them in the embedder to remelt?? Or should something else be done to preserve them.
Thanks

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas? 78744
(512)901-0900 ext. 6912

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From pruegg <@t> ihctech.net  Tue Nov 29 12:29:07 2011
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Tue Nov 29 12:29:10 2011
Subject: [Histonet] nuclear fast red
Message-ID: <94C3B2CA09434506BBEB3246C1D686EB@Patsyoffice>

Is my nuclear fast red powder dead or does it take a long time with heat and
stirring to turn red?  It is pretty old, and one vial says it should be
stored below 0 but has not been.

 

Cheers,

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email   pruegg@ihctech.net
web site   www.ihctech.net

 


This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.

 

From liz <@t> premierlab.com  Tue Nov 29 14:22:21 2011
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Tue Nov 29 14:22:48 2011
Subject: [Histonet] nuclear fast red
In-Reply-To: <94C3B2CA09434506BBEB3246C1D686EB@Patsyoffice>
References: <94C3B2CA09434506BBEB3246C1D686EB@Patsyoffice>
Message-ID: <14E2C6176416974295479C64A11CB9AE011380AD9C8D@SBS2K8.premierlab.local>

Patsy

It does not go completely in solution we filter prior to staining

Liz

________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg [pruegg@ihctech.net]
Sent: Tuesday, November 29, 2011 11:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] nuclear fast red

Is my nuclear fast red powder dead or does it take a long time with heat and
stirring to turn red?  It is pretty old, and one vial says it should be
stored below 0 but has not been.



Cheers,

Patsy



Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email   pruegg@ihctech.net
web site   www.ihctech.net




This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
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From SEsparza <@t> seton.org  Tue Nov 29 14:52:25 2011
From: SEsparza <@t> seton.org (Esparza, Sandra)
Date: Tue Nov 29 14:53:10 2011
Subject: [Histonet] Histologist needed in Austin Texas
Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB041DFE7F@AUSEX2VS1.seton.org>

Full Time/Day Shift position open for a HT/HTL (ASCP) registered
histologist, with 2+ years working experience.  Requires strong
embedding and microtomy skills, knowledge of muscle enzymes, EM, and IHC
is preferred.  Proficiency in these areas can be obtained on the job. We
are a highly Specialized Histology department with state of the art
equipment. This position is at a Trauma 1 children's hospital located in
beautiful progressive Austin Texas.  If you are interested in working in
a team environment please go to Seton.net to apply online.

 

 

Sandra Esparza HT(ASCP)QIHC

sesparza@seton.org

From amosbrooks <@t> gmail.com  Tue Nov 29 15:24:28 2011
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Tue Nov 29 15:24:38 2011
Subject: [Histonet] validation
Message-ID: 

Hi,
    I would say no, and if any inspector disagreed you would be well within
your rights to give him a good cuff upside the head. The point in having a
negative mouse and rabbit is to run them at the same concentration as
whatever primary you are running. That would mean you would need to
validate it at every conceivable dilution you could ever have a primary
antibody at. Kinda silly if you think about it. Any good validation for a
primary antibody should include a proper isotype negative control anyway,
but that is a separate can o' worms.

Amos


On Tue, Nov 29, 2011 at 1:01 PM,
wrote:

> Message: 5
> Date: Mon, 28 Nov 2011 20:00:03 +0000
> From: Amber McKenzie 
> Subject: [Histonet] validation
> To: "histonet@lists.utsouthwestern.edu"
>        
> Message-ID: <5A33C952BB67F4468AF1F36D739212BC0642BE@JERRY.Gia.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Do you have to validate the neg mouse and rabbit?
>
From tp2 <@t> medicine.wisc.edu  Tue Nov 29 16:30:17 2011
From: tp2 <@t> medicine.wisc.edu (Thomas Pier)
Date: Tue Nov 29 16:30:35 2011
Subject: [Histonet] autostainer vials
Message-ID: <4ED5089A020000DF0000C9DF@gwmail.medicine.wisc.edu>

I'm using a Labvision Autostainer 360. I typically wash and reuse the reagent vials. Does anybody out there have any idea how long the vials will last doing this? Is there anything I'm not thinking of that I should be concerned about?

Tom
From Diane.Tokugawa <@t> kp.org  Tue Nov 29 17:02:52 2011
From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org)
Date: Tue Nov 29 17:03:10 2011
Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.
Message-ID: 


I will be out of the office starting  11/29/2011 and will not return until
12/05/2011.

Note:   For Cytology issues, please call Molly  at 8-421-5487,  Eric at
8-421-5405, or Wanda 8-421-5426   For Histology issues, please call Mario
at 8-421-4961, general histology lab 8-421- 5408, Kiran at 8-421-5404,  or
Wanda at 8-421-5426.
From marcia.spencer26 <@t> gmail.com  Tue Nov 29 21:49:10 2011
From: marcia.spencer26 <@t> gmail.com (Marcia Spencer)
Date: Tue Nov 29 21:49:14 2011
Subject: [Histonet] Question regarding cutting on microtome
Message-ID: 

I am wondering what causes a "ribbon" appearance in skin when viewed
under the microscope, usually seen in the epidermis, but occasionally
can be seen in the underlying tissue as well.  I find this effect
happens only in a few, 4 or 5 slides out of 100, but, I don't know how
to correct it.  I would describe it as the "old fashion hard candy
that was rippled like a ribbon could be.  Under the scope, focusing up
and down demonstrates that the section is complete, just not on the
same plane.  Any suggestions?
Thank you for your time
M. Spencer

On 11/27/11, histonet-request@lists.utsouthwestern.edu
 wrote:
> Send Histonet mailing list submissions to
> 	histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> 	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> 	histonet-request@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> 	histonet-owner@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>    1. dako autostainer labeler (Patsy Ruegg)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sun, 27 Nov 2011 08:55:58 -0700
> From: "Patsy Ruegg" 
> Subject: [Histonet] dako autostainer labeler
> To: 
> Message-ID: 
> Content-Type: text/plain;	charset="us-ascii"
>
> Greetings,
>
>
>
> I am looking for another Seymour labeler like the one used on the Dako
> Autostainer, does anyone have one sitting around not being used I could
> purchase from you?  It needs to have the software disc so I can install it
> on a computer.
>
>
>
> Regards,
>
>
>
> Patsy
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC
>
> IHCtech
>
> 12635 Montview Blvd. Ste.215
>
> Aurora, CO 80045
>
> 720-859-4060
>
> fax 720-859-4110
>
> www.ihctech.net
>
> www.ihcrg.org
>
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 96, Issue 39
> ****************************************
>

From JCBRITTON <@t> Cheshire-Med.COM  Wed Nov 30 08:08:42 2011
From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C)
Date: Wed Nov 30 08:08:51 2011
Subject: [Histonet] Question regarding cutting on microtome
In-Reply-To: 
References: 
Message-ID: 

Your waterbath is not hot enough!

 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia
Spencer
Sent: Tuesday, November 29, 2011 10:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question regarding cutting on microtome

 

I am wondering what causes a "ribbon" appearance in skin when viewed

under the microscope, usually seen in the epidermis, but occasionally

can be seen in the underlying tissue as well.  I find this effect

happens only in a few, 4 or 5 slides out of 100, but, I don't know how

to correct it.  I would describe it as the "old fashion hard candy

that was rippled like a ribbon could be.  Under the scope, focusing up

and down demonstrates that the section is complete, just not on the

same plane.  Any suggestions?

Thank you for your time

M. Spencer

 

On 11/27/11, histonet-request@lists.utsouthwestern.edu

 wrote:

> Send Histonet mailing list submissions to

>          histonet@lists.utsouthwestern.edu

> 

> To subscribe or unsubscribe via the World Wide Web, visit

>          http://lists.utsouthwestern.edu/mailman/listinfo/histonet

> or, via email, send a message with subject or body 'help' to

>          histonet-request@lists.utsouthwestern.edu

> 

> You can reach the person managing the list at

>          histonet-owner@lists.utsouthwestern.edu

> 

> When replying, please edit your Subject line so it is more specific

> than "Re: Contents of Histonet digest..."

> 

> 

> Today's Topics:

> 

>    1. dako autostainer labeler (Patsy Ruegg)

> 

> 

> ----------------------------------------------------------------------

> 

> Message: 1

> Date: Sun, 27 Nov 2011 08:55:58 -0700

> From: "Patsy Ruegg" 

> Subject: [Histonet] dako autostainer labeler

> To: 

> Message-ID: 

> Content-Type: text/plain;          charset="us-ascii"

> 

> Greetings,

> 

> 

> 

> I am looking for another Seymour labeler like the one used on the Dako

> Autostainer, does anyone have one sitting around not being used I
could

> purchase from you?  It needs to have the software disc so I can
install it

> on a computer.

> 

> 

> 

> Regards,

> 

> 

> 

> Patsy

> 

> 

> 

> Patsy Ruegg, HT(ASCP)QIHC

> 

> IHCtech

> 

> 12635 Montview Blvd. Ste.215

> 

> Aurora, CO 80045

> 

> 720-859-4060

> 

> fax 720-859-4110

> 

> www.ihctech.net

> 

> www.ihcrg.org

> 

> 

> 

> 

> 

> ------------------------------

> 

> _______________________________________________

> Histonet mailing list

> Histonet@lists.utsouthwestern.edu

> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

> 

> End of Histonet Digest, Vol 96, Issue 39

> ****************************************

> 

 

_______________________________________________

Histonet mailing list

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From nto <@t> stowers.org  Wed Nov 30 09:00:49 2011
From: nto <@t> stowers.org (Thomas, Nancy)
Date: Wed Nov 30 09:00:57 2011
Subject: [Histonet] Chameleon tissue lifting off
Message-ID: <2C40E43D1F7A56408C4463FD245DDDF993990281@EXCHMB-02.stowers-institute.org>

I would like to ask anyone who sections chameleon tissue what type of slides they use.  I was having too much lifting while using the superfrost plus slides.  It looked like slides coated with Haupt's solution were highly recommended, so I tried that.  It is so much better, but still there is some lifting.  If someone is successful with sectioning and staining lizard tissue without lifting, please advise me on type of slides, drying times, or anything that might help.
Thank you so much,

Nancy Thomas
Stowers Institute for Medical Research
Kansas City, MO
From JThawley <@t> ShoreMemorial.org  Wed Nov 30 09:19:46 2011
From: JThawley <@t> ShoreMemorial.org (JThawley@ShoreMemorial.org)
Date: Wed Nov 30 09:19:52 2011
Subject: [Histonet] (no subject)
Message-ID: 


Hello All,

We are having a issue with our intercom system in the OR so the pathologist
are having issues communicating frozen section diagnosis with the surgeon.
Does anyone in Histoland use a written form to send frozen section
diagnosis? Per CAP if it is verbal the pathologist must speak directly with
the surgeon.  Any suggestions would be much appreciated.


Jennifer Thawley HT, ASCP
Histology Supervisor
Shore Memorial Hospital
(609) 653-3940


This transmittal from Shore Memorial Health System is for the sole use of
the intended recipient and may contain confidential and privileged
information.  Any unauthorized review or use, including disclosure or
distribution is prohibited.  If you are not the intended recipient, please
contact the sender and destroy all copies of the transmittal.


From trathborne <@t> somerset-healthcare.com  Wed Nov 30 09:56:19 2011
From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni)
Date: Wed Nov 30 09:56:33 2011
Subject: [Histonet] (no subject)
In-Reply-To: 
References: 
Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570711F62FCD@SMCMAIL01.somerset-healthcare.com>

We switched from intercoms to phones some years back. The phone is put on speaker mode in the OR. I suppose problems can occur with the phone system too, but we haven't experienced this.
Regarding the CAP part of the question, I would call them. Ask if you can send a written diagnosis, which you are to receive back with the physician's/designee's signature. You can then document the intercom problems, and how you worked around it.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JThawley@ShoreMemorial.org
Sent: Wednesday, November 30, 2011 10:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)


Hello All,

We are having a issue with our intercom system in the OR so the pathologist are having issues communicating frozen section diagnosis with the surgeon.
Does anyone in Histoland use a written form to send frozen section diagnosis? Per CAP if it is verbal the pathologist must speak directly with the surgeon.  Any suggestions would be much appreciated.


Jennifer Thawley HT, ASCP
Histology Supervisor
Shore Memorial Hospital
(609) 653-3940


This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information.  Any unauthorized review or use, including disclosure or distribution is prohibited.  If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal.


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From wdesalvo.cac <@t> hotmail.com  Wed Nov 30 10:29:37 2011
From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO)
Date: Wed Nov 30 10:29:43 2011
Subject: [Histonet] Chameleon tissue lifting off
In-Reply-To: <2C40E43D1F7A56408C4463FD245DDDF993990281@EXCHMB-02.stowers-institute.org>
References: <2C40E43D1F7A56408C4463FD245DDDF993990281@EXCHMB-02.stowers-institute.org>
Message-ID: 


I worked w/ animal tissue, not specifically lizard, several years ago and we always used Poly-L-Lysine coated slides. I found the polycationic nature of this molecule seemed to interact well w/ non-human species and created a strong interaction with the anionic sites of animal tissue sections. Poly-L-Lysine always produced strong adhesive properties. We also dried the sections at room temperature for 24 hours as we found that longer and slower worked best for us.

William DeSalvo, B.S., HTL(ASCP)

 

> From: nto@stowers.org
> To: histonet@lists.utsouthwestern.edu
> Date: Wed, 30 Nov 2011 09:00:49 -0600
> Subject: [Histonet] Chameleon tissue lifting off
> 
> I would like to ask anyone who sections chameleon tissue what type of slides they use. I was having too much lifting while using the superfrost plus slides. It looked like slides coated with Haupt's solution were highly recommended, so I tried that. It is so much better, but still there is some lifting. If someone is successful with sectioning and staining lizard tissue without lifting, please advise me on type of slides, drying times, or anything that might help.
> Thank you so much,
> 
> Nancy Thomas
> Stowers Institute for Medical Research
> Kansas City, MO
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From christiegowan <@t> msn.com  Wed Nov 30 10:51:18 2011
From: christiegowan <@t> msn.com (CHRISTIE GOWAN)
Date: Wed Nov 30 10:51:23 2011
Subject: [Histonet] Immunohistochemical staining of type IV collagen in
 Alport's Syndrome
Message-ID: 


We are looking into the feasability of doing this test in-house. Is anyone doing this test for kidney and if so, how did you run your validation?  We have some positive patients but they are all skin so I guess we will need to purchase controls specifically for kidney. If anyone has any experience with this I would appreciate your comments. Thanks.
Christie Gowan
UAB University Hospital 		 	   		  
From histology.houston <@t> yahoo.com  Wed Nov 30 10:56:17 2011
From: histology.houston <@t> yahoo.com (Maria T)
Date: Wed Nov 30 10:56:19 2011
Subject: [Histonet] Part-time Job
Message-ID: <1322672177.64453.YahooMailNeo@web140502.mail.bf1.yahoo.com>

Needed a PRN for a small GI Lab in Houston? The position is one week a month from 2-10pm depending on workload.?
Position includes grossing BX?s, processing, embedding and cutting.?
If interested in position please email your resume along with any questions.

Thanks
From Ronald.Houston <@t> nationwidechildrens.org  Wed Nov 30 11:10:12 2011
From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald)
Date: Wed Nov 30 11:10:23 2011
Subject: [Histonet] fresh specimens after hours
Message-ID: 

for facilities that are NOT staffed 24/7, how are fresh specimens handled after hours?

Up till now, OR staff have called the pathologist on-call to determine how best to handle the specimen, but that process has broken down on the OR side of things. The OR Director does not want 2 different ways of dealing with fresh specimens and they now want a process that will be the same 24/7.

As we will not be staffing the lab 24/7 on the odd chance a fresh specimen might come, I am interested in what other facilities may be doing.

Thanks
Ronnie


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster



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From joelleweaver <@t> hotmail.com  Wed Nov 30 11:23:00 2011
From: joelleweaver <@t> hotmail.com (joelle weaver)
Date: Wed Nov 30 11:23:05 2011
Subject: [Histonet] fresh specimens after hours
In-Reply-To: 
References: 
Message-ID: 


RonnieFrom my experience when working in labs that are not staffed 24/7 with HT or grossing personnel, after hours fresh specimens are called to a pathologist on call by the OR staff, who may then make the judgment to instruct them on fixation needed, or come in for a FS or view the specimen. Most places have used a pager or page # system with an on call rotation. Sometimes a histology person has been on -call after hours too ( if they usually do the FS, accessioning etc at the bench in lieu of PA) or just be available certain hours if the pathologist needs them to assist with FS,  other preparations for send outs etc.I think this is pretty much what you have suggested, and that seems to be in alignment with the arrangments and expectations I have worked for these circumstances in a few of my positions without 24 hr staff.Joelle

Joelle Weaver MAOM, BA, (HTL) ASCP
 
http://www.linkedin.com/in/joelleweaver

 > From: Ronald.Houston@nationwidechildrens.org
> To: histonet@lists.utsouthwestern.edu
> Date: Wed, 30 Nov 2011 17:10:12 +0000
> Subject: [Histonet] fresh specimens after hours
> 
> for facilities that are NOT staffed 24/7, how are fresh specimens handled after hours?
> 
> Up till now, OR staff have called the pathologist on-call to determine how best to handle the specimen, but that process has broken down on the OR side of things. The OR Director does not want 2 different ways of dealing with fresh specimens and they now want a process that will be the same 24/7.
> 
> As we will not be staffing the lab 24/7 on the odd chance a fresh specimen might come, I am interested in what other facilities may be doing.
> 
> Thanks
> Ronnie
> 
> 
> Ronnie Houston, MS HT(ASCP)QIHC
> Anatomic Pathology Manager
> ChildLab, a Division of Nationwide Children's Hospital
> www.childlab.com
> 
> 700 Children's Drive
> Columbus, OH 43205
> (P) 614-722-5450
> (F) 614-722-2899
> ronald.houston@nationwidechildrens.org
> www.NationwideChildrens.org
> 
> "One person with passion is better than forty people merely interested."
> ~ E.M. Forster
> 
> 
> 
> ----------------------------------------- Confidentiality Notice:
> The following mail message, including any attachments, is for the
> sole use of the intended recipient(s) and may contain confidential
> and privileged information. The recipient is responsible to
> maintain the confidentiality of this information and to use the
> information only for authorized purposes. If you are not the
> intended recipient (or authorized to receive information for the
> intended recipient), you are hereby notified that any review, use,
> disclosure, distribution, copying, printing, or action taken in
> reliance on the contents of this e-mail is strictly prohibited. If
> you have received this communication in error, please notify us
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From Erin.Martin <@t> ucsf.edu  Wed Nov 30 11:55:14 2011
From: Erin.Martin <@t> ucsf.edu (Martin, Erin)
Date: Wed Nov 30 11:56:59 2011
Subject: [Histonet] ASR FISH probes
Message-ID: <24B7B291CC88D04AB663958E77A1F59D0133E7@ex09.net.ucsf.edu>

Hi all,



More questions on FISH.  How does one go about validating an ASR probe on FFPE tissue?  I have no FISH experience and therefore am starting from scratch.  The specific probes that our pathologists are interested in are:  t(14;18), t(17;22) and t(2;5).



Also, does anyone know what educational and training background is required to be able to do such a workup?  I know IVDs are no problems, I looked at the CAP checklist for molecular and it seems that this is usually done in cytogenetics and the tech needs to at least have a BS and experience with molecular.



Any help would be greatly appreciated!



Thanks,

Erin




From khbarr <@t> mdanderson.org  Wed Nov 30 12:15:25 2011
From: khbarr <@t> mdanderson.org (Barr,Kaye H)
Date: Wed Nov 30 12:15:29 2011
Subject: [Histonet] HT Position Available
Message-ID: 

UT MD Anderson Cancer Center in Houston, Texas has a vacant position for a Clinical Histology Technician The candidate should have experience sectioning paraffin embedded  biopsies.
The shift for this position is either 11:00 pm - 7:30 am or 10:30 pm - 6:30 am Sunday through Thursday.  Apply online at www.mdanderson.org or contact the recruiter Nikol Blackmon at 713-745-6349.



Kaye Barr, HT(ASCP)
Laboratory Manager, PLM
Pathology Dept.
UT M.D. Anderson Cancer Center
Houston, Texas
713-792-5366


From wdesalvo.cac <@t> hotmail.com  Wed Nov 30 12:37:54 2011
From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO)
Date: Wed Nov 30 12:37:58 2011
Subject: [Histonet] fresh specimens after hours
In-Reply-To: 
References: 
Message-ID: 


After Surgical Pathology closes, consider having all specimens taken to the clinical lab (staffed 24/7) and have them check in all specimens. If a FS is needed, they can contact the on-call pathologists and tech. If no FS, they can have instructions, by tissue type, to add the correct fixative or just refrigerate. Surgical Path staff picks up specimens from the clin lab  the next morning.    

William DeSalvo, B.S., HTL(ASCP)

 

> From: Ronald.Houston@nationwidechildrens.org
> To: histonet@lists.utsouthwestern.edu
> Date: Wed, 30 Nov 2011 17:10:12 +0000
> Subject: [Histonet] fresh specimens after hours
> 
> for facilities that are NOT staffed 24/7, how are fresh specimens handled after hours?
> 
> Up till now, OR staff have called the pathologist on-call to determine how best to handle the specimen, but that process has broken down on the OR side of things. The OR Director does not want 2 different ways of dealing with fresh specimens and they now want a process that will be the same 24/7.
> 
> As we will not be staffing the lab 24/7 on the odd chance a fresh specimen might come, I am interested in what other facilities may be doing.
> 
> Thanks
> Ronnie
> 
> 
> Ronnie Houston, MS HT(ASCP)QIHC
> Anatomic Pathology Manager
> ChildLab, a Division of Nationwide Children's Hospital
> www.childlab.com
> 
> 700 Children's Drive
> Columbus, OH 43205
> (P) 614-722-5450
> (F) 614-722-2899
> ronald.houston@nationwidechildrens.org
> www.NationwideChildrens.org
> 
> "One person with passion is better than forty people merely interested."
> ~ E.M. Forster
> 
> 
> 
> ----------------------------------------- Confidentiality Notice:
> The following mail message, including any attachments, is for the
> sole use of the intended recipient(s) and may contain confidential
> and privileged information. The recipient is responsible to
> maintain the confidentiality of this information and to use the
> information only for authorized purposes. If you are not the
> intended recipient (or authorized to receive information for the
> intended recipient), you are hereby notified that any review, use,
> disclosure, distribution, copying, printing, or action taken in
> reliance on the contents of this e-mail is strictly prohibited. If
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From ihcman2010 <@t> hotmail.com  Wed Nov 30 12:52:35 2011
From: ihcman2010 <@t> hotmail.com (Glen Dawson)
Date: Wed Nov 30 12:52:41 2011
Subject: [Histonet] Histology Jobs in Janesville, WI
In-Reply-To: <1322672177.64453.YahooMailNeo@web140502.mail.bf1.yahoo.com>
References: <1322672177.64453.YahooMailNeo@web140502.mail.bf1.yahoo.com>
Message-ID: 



All,
 
Mercy Hospital and Trauma Center in Janesville, WI has openings for a full-time and a pool histotechnician.  Please visit www.mercyhealthsystem.org for more details.
 
Thank-you,
 
Glen Dawson  BS, HT(ASCP) & QIHC
Histology Technical Specialist
Mercy Hospital and Trauma Center
Janesville, WI 		 	   		  
From thiggins <@t> cddmedical.com  Wed Nov 30 13:28:53 2011
From: thiggins <@t> cddmedical.com (Tim Higgins)
Date: Wed Nov 30 13:28:57 2011
Subject: Subject: [Histonet] nuclear fast red
References: <20111130180428.0FA3E13D2A5A@barracuda.crvinc.net>
Message-ID: <005501ccaf96$4bc35730$e001a8c0@cdd.loc>

Nuclear Fast Red needs to be heated during prep and it does take a little
while to dissolve, it never really dissolves entirely, probably saturates
the solution pretty fast.

Tim

Message: 1
Date: Tue, 29 Nov 2011 11:29:07 -0700
From: "Patsy Ruegg" 
Subject: [Histonet] nuclear fast red
To: 
Message-ID: <94C3B2CA09434506BBEB3246C1D686EB@Patsyoffice>
Content-Type: text/plain; charset="us-ascii"

Is my nuclear fast red powder dead or does it take a long time with heat and
stirring to turn red?  It is pretty old, and one vial says it should be
stored below 0 but has not been.



Cheers,

Patsy



Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email   pruegg@ihctech.net
web site   www.ihctech.net






From dmlongoria <@t> ecrmc.org  Wed Nov 30 14:08:01 2011
From: dmlongoria <@t> ecrmc.org (Diana Martinez-Longoria)
Date: Wed Nov 30 14:08:09 2011
Subject: [Histonet] ASCP membership
Message-ID: <81487B04-08E0-4A91-92A9-5B40A63BA812@mimectl>

Hello all,



I was wondering if the ASCP membership is worth enrolling, especially since it is a little pricey but it can also keep track with regards too CMP's. Your input would be very beneficial. Thank you ahead for your time.



Diana Martinez-Longoria

Histotechnician (ASCP)cm

El Centro Regional Medical Center

phone: 760-339-7267

fax: 760-482-5365

email:dmlongoria@ecrmc.org




ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.
??


From esarricks <@t> gmail.com  Wed Nov 30 14:23:27 2011
From: esarricks <@t> gmail.com (Erin Sarricks)
Date: Wed Nov 30 14:23:33 2011
Subject: [Histonet] MAP-2 Troubleshooting
Message-ID: 

Hi Histonet-



I am posting this question for a colleague of mine who is having some
problems performing a MAP-2 stain.





Problem:  Blotchy or "wormy" areas of light or absent staining of MAP-2
(Thermo Scientific MS-250-R7) in brain sections by immunohistochemistry.
Microwave antigen retrieval was performed using 10 mM citric acid at pH 6.
The stain worked very well historically. Charged slides were stained by
immersion or individually using PAP barrier. The areas of stain loss are
repetitive on neighboring slides, which suggests antigen retrieval as the
problem step. Several attempts at modifying the retrieval process have not
been unsuccessful.




Has anyone else been running into these issues?  Any advice or tips would
be greatly appreciated!  Thank you.



Erin Sarricks, HT (ASCP)

Histology Team Leader

USAMRICD Comparative Pathology Branch

Office: Bldg E-3081 Room 178

E-mail: erin.p.sarricks@us.army.mil

Phone: 410-436-1967
From Kay.Nabers <@t> trumbulllabs.com  Wed Nov 30 14:54:03 2011
From: Kay.Nabers <@t> trumbulllabs.com (Kay W. Nabers)
Date: Wed Nov 30 14:54:09 2011
Subject: [Histonet] Histology Opening
Message-ID: 

  Trumbull Laboratories, private lab, in Germantown, Tn has an opening for a full time ASCP registered  histotech, day shift, M_F with Saturday rotation. Experience  desired with routine and biopsy tissues, IHC, special stains, and Fluorescence  Interested applicants apply online at trumbulllabs.com.
From rjbuesa <@t> yahoo.com  Wed Nov 30 14:54:37 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Nov 30 14:54:41 2011
Subject: [Histonet] ASCP membership
In-Reply-To: <81487B04-08E0-4A91-92A9-5B40A63BA812@mimectl>
Message-ID: <1322686477.1773.YahooMailClassic@web65712.mail.ac4.yahoo.com>

I do not think it is worth enrolling, at least from the histology subject point of view.
Check the contents of several issue and you will realize that articles about histology are very few, I think that less 1 in 25, if that many.
I had?subscribed and unsubscribed at least twice.
Ren? J.

--- On Wed, 11/30/11, Diana Martinez-Longoria  wrote:


From: Diana Martinez-Longoria 
Subject: [Histonet] ASCP membership
To: "histonet@lists.utsouthwestern.edu" 
Date: Wednesday, November 30, 2011, 3:08 PM


Hello all,



I was wondering if the ASCP membership is worth enrolling, especially since it is a little pricey but it can also keep track with regards too CMP's. Your input would be very beneficial. Thank you ahead for your time.



Diana Martinez-Longoria

Histotechnician (ASCP)cm

El Centro Regional Medical Center

phone: 760-339-7267

fax: 760-482-5365

email:dmlongoria@ecrmc.org




ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.
??


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Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From Wanda.Smith <@t> HCAhealthcare.com  Wed Nov 30 16:06:34 2011
From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com)
Date: Wed Nov 30 16:06:40 2011
Subject: [Histonet] RE: fresh specimens after hours
In-Reply-To: 
References: 
Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA2734104396@NADCWPMSGCMS03.hca.corpad.net>

The Pathologist on call handles these calls.

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald
Sent: Wednesday, November 30, 2011 12:10 PM
To: 'histo net'
Subject: [Histonet] fresh specimens after hours

for facilities that are NOT staffed 24/7, how are fresh specimens handled after hours?

Up till now, OR staff have called the pathologist on-call to determine how best to handle the specimen, but that process has broken down on the OR side of things. The OR Director does not want 2 different ways of dealing with fresh specimens and they now want a process that will be the same 24/7.

As we will not be staffing the lab 24/7 on the odd chance a fresh specimen might come, I am interested in what other facilities may be doing.

Thanks
Ronnie


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster



----------------------------------------- Confidentiality Notice:
The following mail message, including any attachments, is for the
sole use of the intended recipient(s) and may contain confidential
and privileged information. The recipient is responsible to
maintain the confidentiality of this information and to use the
information only for authorized purposes. If you are not the
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intended recipient), you are hereby notified that any review, use,
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From Sharon.Genest <@t> saskatoonhealthregion.ca  Wed Nov 30 16:43:37 2011
From: Sharon.Genest <@t> saskatoonhealthregion.ca (Genest, Sharon  SktnHR)
Date: Wed Nov 30 16:44:12 2011
Subject: [Histonet] Case Distribution
Message-ID: 

Can anyone provide me with inforrmation regarding how you distribute
cases to pathologists. I work for a region which has cases coming in
from many sites and pathologists reading cases from three sites. Our
caseload is approx 35,000 cases per year of varying specimen types.  We
are looking at changing how we distribute cases right now I am just
fishing for ideas to make sure that we consider different ideas and
hopefully come up with a process that meets our needs.
Thanks

Sharon 

From m.kap.1 <@t> erasmusmc.nl  Wed Nov 30 16:45:04 2011
From: m.kap.1 <@t> erasmusmc.nl (M. Kap)
Date: Wed Nov 30 16:45:17 2011
Subject: [Histonet] Re: Histonet Digest, Vol 96, Issue 42
In-Reply-To: 
References: 
Message-ID: <756ac227cf41dd465602c8c8b9065d58.squirrel@webmail.erasmusmc.nl>

@15

Wrap tissue in moist, not too wet!!,  (0.9% salt or PBS) gauze and store
at 4 degrees C. No/hardly any harm done to morphology, antigenicity, RNA,
DNA and so on. Tissue is tougher than you think...

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0027704


> Send Histonet mailing list submissions to
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>
> Today's Topics:
>
>    1. nuclear fast red (Patsy Ruegg)
>    2. RE: nuclear fast red (Elizabeth Chlipala)
>    3. Histologist needed in Austin Texas (Esparza, Sandra)
>    4. validation (Amos Brooks)
>    5. autostainer vials (Thomas Pier)
>    6. Diane Tokugawa/CA/KAIPERM is out of the office.
>       (Diane.Tokugawa@kp.org)
>    7. Question regarding cutting on microtome (Marcia Spencer)
>    8. RE: Question regarding cutting on microtome (Britton, Josette C)
>    9. Chameleon tissue lifting off (Thomas, Nancy)
>   10. (no subject) (JThawley@ShoreMemorial.org)
>   11. RE: (no subject) (Rathborne, Toni)
>   12. RE: Chameleon tissue lifting off (WILLIAM DESALVO)
>   13. Immunohistochemical staining of type IV collagen in Alport's
>       Syndrome (CHRISTIE GOWAN)
>   14. Part-time Job (Maria T)
>   15. fresh specimens after hours (Houston, Ronald)
>   16. RE: fresh specimens after hours (joelle weaver)
>   17. ASR FISH probes (Martin, Erin)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 29 Nov 2011 11:29:07 -0700
> From: "Patsy Ruegg" 
> Subject: [Histonet] nuclear fast red
> To: 
> Message-ID: <94C3B2CA09434506BBEB3246C1D686EB@Patsyoffice>
> Content-Type: text/plain;	charset="us-ascii"
>
> Is my nuclear fast red powder dead or does it take a long time with heat
> and
> stirring to turn red?  It is pretty old, and one vial says it should be
> stored below 0 but has not been.
>
>
>
> Cheers,
>
> Patsy
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email   pruegg@ihctech.net
> web site   www.ihctech.net
>
>
>
>
> This email is confidential and intended solely for the use of the
> Person(s)
> ('the intended recipient') to whom it was addressed. Any views or opinions
> presented are solely those of the author. It may contain information that
> is
> privileged & confidential within the meaning of applicable law.
> Accordingly
> any dissemination, distribution, copying, or other use of this message, or
> any of its contents, by any person other than the intended recipient may
> constitute a breach of civil or criminal law and is strictly prohibited.
> If
> you are NOT the intended recipient please contact the sender and dispose
> of
> this e-mail as soon as possible.
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 29 Nov 2011 13:22:21 -0700
> From: Elizabeth Chlipala 
> Subject: RE: [Histonet] nuclear fast red
> To: Patsy Ruegg ,
> 	"histonet@lists.utsouthwestern.edu"
> 	
> Message-ID:
> 	<14E2C6176416974295479C64A11CB9AE011380AD9C8D@SBS2K8.premierlab.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Patsy
>
> It does not go completely in solution we filter prior to staining
>
> Liz
>
> ________________________________________
> From: histonet-bounces@lists.utsouthwestern.edu
> [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
> [pruegg@ihctech.net]
> Sent: Tuesday, November 29, 2011 11:29 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] nuclear fast red
>
> Is my nuclear fast red powder dead or does it take a long time with heat
> and
> stirring to turn red?  It is pretty old, and one vial says it should be
> stored below 0 but has not been.
>
>
>
> Cheers,
>
> Patsy
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email   pruegg@ihctech.net
> web site   www.ihctech.net
>
>
>
>
> This email is confidential and intended solely for the use of the
> Person(s)
> ('the intended recipient') to whom it was addressed. Any views or opinions
> presented are solely those of the author. It may contain information that
> is
> privileged & confidential within the meaning of applicable law.
> Accordingly
> any dissemination, distribution, copying, or other use of this message, or
> any of its contents, by any person other than the intended recipient may
> constitute a breach of civil or criminal law and is strictly prohibited.
> If
> you are NOT the intended recipient please contact the sender and dispose
> of
> this e-mail as soon as possible.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 29 Nov 2011 14:52:25 -0600
> From: "Esparza, Sandra" 
> Subject: [Histonet] Histologist needed in Austin Texas
> To: 
> Message-ID:
> 	<3D79F47DC92B204F9E5D35C885DFC5CB041DFE7F@AUSEX2VS1.seton.org>
> Content-Type: text/plain;	charset="us-ascii"
>
> Full Time/Day Shift position open for a HT/HTL (ASCP) registered
> histologist, with 2+ years working experience.  Requires strong
> embedding and microtomy skills, knowledge of muscle enzymes, EM, and IHC
> is preferred.  Proficiency in these areas can be obtained on the job. We
> are a highly Specialized Histology department with state of the art
> equipment. This position is at a Trauma 1 children's hospital located in
> beautiful progressive Austin Texas.  If you are interested in working in
> a team environment please go to Seton.net to apply online.
>
>
>
>
>
> Sandra Esparza HT(ASCP)QIHC
>
> sesparza@seton.org
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 29 Nov 2011 16:24:28 -0500
> From: Amos Brooks 
> Subject: [Histonet] validation
> To: histonet@lists.utsouthwestern.edu, amber.mckenzie@gastrodocs.net
> Message-ID:
> 	
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi,
>     I would say no, and if any inspector disagreed you would be well
> within
> your rights to give him a good cuff upside the head. The point in having a
> negative mouse and rabbit is to run them at the same concentration as
> whatever primary you are running. That would mean you would need to
> validate it at every conceivable dilution you could ever have a primary
> antibody at. Kinda silly if you think about it. Any good validation for a
> primary antibody should include a proper isotype negative control anyway,
> but that is a separate can o' worms.
>
> Amos
>
>
> On Tue, Nov 29, 2011 at 1:01 PM,
> wrote:
>
>> Message: 5
>> Date: Mon, 28 Nov 2011 20:00:03 +0000
>> From: Amber McKenzie 
>> Subject: [Histonet] validation
>> To: "histonet@lists.utsouthwestern.edu"
>>        
>> Message-ID: <5A33C952BB67F4468AF1F36D739212BC0642BE@JERRY.Gia.com>
>> Content-Type: text/plain; charset="us-ascii"
>>
>> Do you have to validate the neg mouse and rabbit?
>>
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 29 Nov 2011 16:30:17 -0600
> From: "Thomas Pier" 
> Subject: [Histonet] autostainer vials
> To: 
> Message-ID: <4ED5089A020000DF0000C9DF@gwmail.medicine.wisc.edu>
> Content-Type: text/plain; charset=US-ASCII
>
> I'm using a Labvision Autostainer 360. I typically wash and reuse the
> reagent vials. Does anybody out there have any idea how long the vials
> will last doing this? Is there anything I'm not thinking of that I should
> be concerned about?
>
> Tom
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 29 Nov 2011 15:02:52 -0800
> From: Diane.Tokugawa@kp.org
> Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> 	
> Content-Type: text/plain; charset=US-ASCII
>
>
> I will be out of the office starting  11/29/2011 and will not return until
> 12/05/2011.
>
> Note:   For Cytology issues, please call Molly  at 8-421-5487,  Eric at
> 8-421-5405, or Wanda 8-421-5426   For Histology issues, please call Mario
> at 8-421-4961, general histology lab 8-421- 5408, Kiran at 8-421-5404,  or
> Wanda at 8-421-5426.
>
> ------------------------------
>
> Message: 7
> Date: Tue, 29 Nov 2011 22:49:10 -0500
> From: Marcia Spencer 
> Subject: [Histonet] Question regarding cutting on microtome
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> 	
> Content-Type: text/plain; charset=ISO-8859-1
>
> I am wondering what causes a "ribbon" appearance in skin when viewed
> under the microscope, usually seen in the epidermis, but occasionally
> can be seen in the underlying tissue as well.  I find this effect
> happens only in a few, 4 or 5 slides out of 100, but, I don't know how
> to correct it.  I would describe it as the "old fashion hard candy
> that was rippled like a ribbon could be.  Under the scope, focusing up
> and down demonstrates that the section is complete, just not on the
> same plane.  Any suggestions?
> Thank you for your time
> M. Spencer
>
> On 11/27/11, histonet-request@lists.utsouthwestern.edu
>  wrote:
>> Send Histonet mailing list submissions to
>> 	histonet@lists.utsouthwestern.edu
>>
>> To subscribe or unsubscribe via the World Wide Web, visit
>> 	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> or, via email, send a message with subject or body 'help' to
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>> You can reach the person managing the list at
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>>
>> When replying, please edit your Subject line so it is more specific
>> than "Re: Contents of Histonet digest..."
>>
>>
>> Today's Topics:
>>
>>    1. dako autostainer labeler (Patsy Ruegg)
>>
>>
>> ----------------------------------------------------------------------
>>
>> Message: 1
>> Date: Sun, 27 Nov 2011 08:55:58 -0700
>> From: "Patsy Ruegg" 
>> Subject: [Histonet] dako autostainer labeler
>> To: 
>> Message-ID: 
>> Content-Type: text/plain;	charset="us-ascii"
>>
>> Greetings,
>>
>>
>>
>> I am looking for another Seymour labeler like the one used on the Dako
>> Autostainer, does anyone have one sitting around not being used I could
>> purchase from you?  It needs to have the software disc so I can install
>> it
>> on a computer.
>>
>>
>>
>> Regards,
>>
>>
>>
>> Patsy
>>
>>
>>
>> Patsy Ruegg, HT(ASCP)QIHC
>>
>> IHCtech
>>
>> 12635 Montview Blvd. Ste.215
>>
>> Aurora, CO 80045
>>
>> 720-859-4060
>>
>> fax 720-859-4110
>>
>> www.ihctech.net
>>
>> www.ihcrg.org
>>
>>
>>
>>
>>
>> ------------------------------
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> End of Histonet Digest, Vol 96, Issue 39
>> ****************************************
>>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Wed, 30 Nov 2011 09:08:42 -0500
> From: "Britton, Josette C" 
> Subject: RE: [Histonet] Question regarding cutting on microtome
> To: "Marcia Spencer" ,
> 	
> Message-ID:
> 	
> Content-Type: text/plain;	charset="us-ascii"
>
> Your waterbath is not hot enough!
>
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia
> Spencer
> Sent: Tuesday, November 29, 2011 10:49 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Question regarding cutting on microtome
>
>
>
> I am wondering what causes a "ribbon" appearance in skin when viewed
>
> under the microscope, usually seen in the epidermis, but occasionally
>
> can be seen in the underlying tissue as well.  I find this effect
>
> happens only in a few, 4 or 5 slides out of 100, but, I don't know how
>
> to correct it.  I would describe it as the "old fashion hard candy
>
> that was rippled like a ribbon could be.  Under the scope, focusing up
>
> and down demonstrates that the section is complete, just not on the
>
> same plane.  Any suggestions?
>
> Thank you for your time
>
> M. Spencer
>
>
>
> On 11/27/11, histonet-request@lists.utsouthwestern.edu
>
>  wrote:
>
>> Send Histonet mailing list submissions to
>
>>          histonet@lists.utsouthwestern.edu
>
>>
>
>> To subscribe or unsubscribe via the World Wide Web, visit
>
>>          http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>> or, via email, send a message with subject or body 'help' to
>
>>          histonet-request@lists.utsouthwestern.edu
>
>>
>
>> You can reach the person managing the list at
>
>>          histonet-owner@lists.utsouthwestern.edu
>
>>
>
>> When replying, please edit your Subject line so it is more specific
>
>> than "Re: Contents of Histonet digest..."
>
>>
>
>>
>
>> Today's Topics:
>
>>
>
>>    1. dako autostainer labeler (Patsy Ruegg)
>
>>
>
>>
>
>> ----------------------------------------------------------------------
>
>>
>
>> Message: 1
>
>> Date: Sun, 27 Nov 2011 08:55:58 -0700
>
>> From: "Patsy Ruegg" 
>
>> Subject: [Histonet] dako autostainer labeler
>
>> To: 
>
>> Message-ID: 
>
>> Content-Type: text/plain;          charset="us-ascii"
>
>>
>
>> Greetings,
>
>>
>
>>
>
>>
>
>> I am looking for another Seymour labeler like the one used on the Dako
>
>> Autostainer, does anyone have one sitting around not being used I
> could
>
>> purchase from you?  It needs to have the software disc so I can
> install it
>
>> on a computer.
>
>>
>
>>
>
>>
>
>> Regards,
>
>>
>
>>
>
>>
>
>> Patsy
>
>>
>
>>
>
>>
>
>> Patsy Ruegg, HT(ASCP)QIHC
>
>>
>
>> IHCtech
>
>>
>
>> 12635 Montview Blvd. Ste.215
>
>>
>
>> Aurora, CO 80045
>
>>
>
>> 720-859-4060
>
>>
>
>> fax 720-859-4110
>
>>
>
>> www.ihctech.net
>
>>
>
>> www.ihcrg.org
>
>>
>
>>
>
>>
>
>>
>
>>
>
>> ------------------------------
>
>>
>
>> _______________________________________________
>
>> Histonet mailing list
>
>> Histonet@lists.utsouthwestern.edu
>
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>>
>
>> End of Histonet Digest, Vol 96, Issue 39
>
>> ****************************************
>
>>
>
>
>
> _______________________________________________
>
> Histonet mailing list
>
> Histonet@lists.utsouthwestern.edu
>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 9
> Date: Wed, 30 Nov 2011 09:00:49 -0600
> From: "Thomas, Nancy" 
> Subject: [Histonet] Chameleon tissue lifting off
> To: "histonet@lists.utsouthwestern.edu"
> 	
> Message-ID:
> 	<2C40E43D1F7A56408C4463FD245DDDF993990281@EXCHMB-02.stowers-institute.org>
>
> Content-Type: text/plain; charset="us-ascii"
>
> I would like to ask anyone who sections chameleon tissue what type of
> slides they use.  I was having too much lifting while using the superfrost
> plus slides.  It looked like slides coated with Haupt's solution were
> highly recommended, so I tried that.  It is so much better, but still
> there is some lifting.  If someone is successful with sectioning and
> staining lizard tissue without lifting, please advise me on type of
> slides, drying times, or anything that might help.
> Thank you so much,
>
> Nancy Thomas
> Stowers Institute for Medical Research
> Kansas City, MO
>
>
> ------------------------------
>
> Message: 10
> Date: Wed, 30 Nov 2011 10:19:46 -0500
> From: JThawley@ShoreMemorial.org
> Subject: [Histonet] (no subject)
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> 	
>
> Content-Type: text/plain; charset=US-ASCII
>
>
> Hello All,
>
> We are having a issue with our intercom system in the OR so the
> pathologist
> are having issues communicating frozen section diagnosis with the surgeon.
> Does anyone in Histoland use a written form to send frozen section
> diagnosis? Per CAP if it is verbal the pathologist must speak directly
> with
> the surgeon.  Any suggestions would be much appreciated.
>
>
> Jennifer Thawley HT, ASCP
> Histology Supervisor
> Shore Memorial Hospital
> (609) 653-3940
>
>
> This transmittal from Shore Memorial Health System is for the sole use of
> the intended recipient and may contain confidential and privileged
> information.  Any unauthorized review or use, including disclosure or
> distribution is prohibited.  If you are not the intended recipient, please
> contact the sender and destroy all copies of the transmittal.
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Wed, 30 Nov 2011 15:56:19 +0000
> From: "Rathborne, Toni" 
> Subject: RE: [Histonet] (no subject)
> To: "'JThawley@ShoreMemorial.org'" ,
> 	"histonet@lists.utsouthwestern.edu"
> 	
> Message-ID:
> 	<3AD061FE740D464FAC7BF6B5CFB7570711F62FCD@SMCMAIL01.somerset-healthcare.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> We switched from intercoms to phones some years back. The phone is put on
> speaker mode in the OR. I suppose problems can occur with the phone system
> too, but we haven't experienced this.
> Regarding the CAP part of the question, I would call them. Ask if you can
> send a written diagnosis, which you are to receive back with the
> physician's/designee's signature. You can then document the intercom
> problems, and how you worked around it.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> JThawley@ShoreMemorial.org
> Sent: Wednesday, November 30, 2011 10:20 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
>
> Hello All,
>
> We are having a issue with our intercom system in the OR so the
> pathologist are having issues communicating frozen section diagnosis with
> the surgeon.
> Does anyone in Histoland use a written form to send frozen section
> diagnosis? Per CAP if it is verbal the pathologist must speak directly
> with the surgeon.  Any suggestions would be much appreciated.
>
>
> Jennifer Thawley HT, ASCP
> Histology Supervisor
> Shore Memorial Hospital
> (609) 653-3940
>
>
> This transmittal from Shore Memorial Health System is for the sole use of
> the intended recipient and may contain confidential and privileged
> information.  Any unauthorized review or use, including disclosure or
> distribution is prohibited.  If you are not the intended recipient, please
> contact the sender and destroy all copies of the transmittal.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> CONFIDENTIALITY NOTICE
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> Be sure to visit Somerset Medical Center's Web site -
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>
>
>
> ------------------------------
>
> Message: 12
> Date: Wed, 30 Nov 2011 09:29:37 -0700
> From: WILLIAM DESALVO 
> Subject: RE: [Histonet] Chameleon tissue lifting off
> To: , histonet 
> Message-ID: 
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> I worked w/ animal tissue, not specifically lizard, several years ago and
> we always used Poly-L-Lysine coated slides. I found the polycationic
> nature of this molecule seemed to interact well w/ non-human species and
> created a strong interaction with the anionic sites of animal tissue
> sections. Poly-L-Lysine always produced strong adhesive properties. We
> also dried the sections at room temperature for 24 hours as we found that
> longer and slower worked best for us.
>
> William DeSalvo, B.S., HTL(ASCP)
>
>
>
>> From: nto@stowers.org
>> To: histonet@lists.utsouthwestern.edu
>> Date: Wed, 30 Nov 2011 09:00:49 -0600
>> Subject: [Histonet] Chameleon tissue lifting off
>>
>> I would like to ask anyone who sections chameleon tissue what type of
>> slides they use. I was having too much lifting while using the
>> superfrost plus slides. It looked like slides coated with Haupt's
>> solution were highly recommended, so I tried that. It is so much better,
>> but still there is some lifting. If someone is successful with
>> sectioning and staining lizard tissue without lifting, please advise me
>> on type of slides, drying times, or anything that might help.
>> Thank you so much,
>>
>> Nancy Thomas
>> Stowers Institute for Medical Research
>> Kansas City, MO
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 13
> Date: Wed, 30 Nov 2011 16:51:18 +0000
> From: CHRISTIE GOWAN 
> Subject: [Histonet] Immunohistochemical staining of type IV collagen
> 	in Alport's Syndrome
> To: 
> Message-ID: 
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> We are looking into the feasability of doing this test in-house. Is anyone
> doing this test for kidney and if so, how did you run your validation?  We
> have some positive patients but they are all skin so I guess we will need
> to purchase controls specifically for kidney. If anyone has any experience
> with this I would appreciate your comments. Thanks.
> Christie Gowan
> UAB University Hospital
>
> ------------------------------
>
> Message: 14
> Date: Wed, 30 Nov 2011 08:56:17 -0800 (PST)
> From: Maria T 
> Subject: [Histonet] Part-time Job
> To: "histonet@lists.utsouthwestern.edu"
> 	
> Message-ID:
> 	<1322672177.64453.YahooMailNeo@web140502.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=utf-8
>
> Needed a PRN for a small GI Lab in Houston??? The position is one week a
> month from 2-10pm depending on workload.??
> Position includes grossing BX???s, processing, embedding and cutting.??
> If interested in position please email your resume along with any
> questions.
>
> Thanks
>
>
> ------------------------------
>
> Message: 15
> Date: Wed, 30 Nov 2011 17:10:12 +0000
> From: "Houston, Ronald" 
> Subject: [Histonet] fresh specimens after hours
> To: 'histo net' 
> Message-ID:
> 	
>
> Content-Type: text/plain;	charset="us-ascii"
>
> for facilities that are NOT staffed 24/7, how are fresh specimens handled
> after hours?
>
> Up till now, OR staff have called the pathologist on-call to determine how
> best to handle the specimen, but that process has broken down on the OR
> side of things. The OR Director does not want 2 different ways of dealing
> with fresh specimens and they now want a process that will be the same
> 24/7.
>
> As we will not be staffing the lab 24/7 on the odd chance a fresh specimen
> might come, I am interested in what other facilities may be doing.
>
> Thanks
> Ronnie
>
>
> Ronnie Houston, MS HT(ASCP)QIHC
> Anatomic Pathology Manager
> ChildLab, a Division of Nationwide Children's Hospital
> www.childlab.com
>
> 700 Children's Drive
> Columbus, OH 43205
> (P) 614-722-5450
> (F) 614-722-2899
> ronald.houston@nationwidechildrens.org
> www.NationwideChildrens.org
>
> "One person with passion is better than forty people merely interested."
> ~ E.M. Forster
>
>
>
> ----------------------------------------- Confidentiality Notice:
> The following mail message, including any attachments, is for the
> sole use of the intended recipient(s) and may contain confidential
> and privileged information. The recipient is responsible to
> maintain the confidentiality of this information and to use the
> information only for authorized purposes. If you are not the
> intended recipient (or authorized to receive information for the
> intended recipient), you are hereby notified that any review, use,
> disclosure, distribution, copying, printing, or action taken in
> reliance on the contents of this e-mail is strictly prohibited. If
> you have received this communication in error, please notify us
> immediately by reply e-mail and destroy all copies of the original
> message. Thank you.
>
> ------------------------------
>
> Message: 16
> Date: Wed, 30 Nov 2011 17:23:00 +0000
> From: joelle weaver 
> Subject: RE: [Histonet] fresh specimens after hours
> To: , Histonet
> 	
> Message-ID: 
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> RonnieFrom my experience when working in labs that are not staffed 24/7
> with HT or grossing personnel, after hours fresh specimens are called to a
> pathologist on call by the OR staff, who may then make the judgment to
> instruct them on fixation needed, or come in for a FS or view the
> specimen. Most places have used a pager or page # system with an on call
> rotation. Sometimes a histology person has been on -call after hours too (
> if they usually do the FS, accessioning etc at the bench in lieu of PA) or
> just be available certain hours if the pathologist needs them to assist
> with FS,  other preparations for send outs etc.I think this is pretty much
> what you have suggested, and that seems to be in alignment with the
> arrangments and expectations I have worked for these circumstances in a
> few of my positions without 24 hr staff.Joelle
>
> Joelle Weaver MAOM, BA, (HTL) ASCP
>
> http://www.linkedin.com/in/joelleweaver
>
>  > From: Ronald.Houston@nationwidechildrens.org
>> To: histonet@lists.utsouthwestern.edu
>> Date: Wed, 30 Nov 2011 17:10:12 +0000
>> Subject: [Histonet] fresh specimens after hours
>>
>> for facilities that are NOT staffed 24/7, how are fresh specimens
>> handled after hours?
>>
>> Up till now, OR staff have called the pathologist on-call to determine
>> how best to handle the specimen, but that process has broken down on the
>> OR side of things. The OR Director does not want 2 different ways of
>> dealing with fresh specimens and they now want a process that will be
>> the same 24/7.
>>
>> As we will not be staffing the lab 24/7 on the odd chance a fresh
>> specimen might come, I am interested in what other facilities may be
>> doing.
>>
>> Thanks
>> Ronnie
>>
>>
>> Ronnie Houston, MS HT(ASCP)QIHC
>> Anatomic Pathology Manager
>> ChildLab, a Division of Nationwide Children's Hospital
>> www.childlab.com
>>
>> 700 Children's Drive
>> Columbus, OH 43205
>> (P) 614-722-5450
>> (F) 614-722-2899
>> ronald.houston@nationwidechildrens.org
>> www.NationwideChildrens.org
>>
>> "One person with passion is better than forty people merely interested."
>> ~ E.M. Forster
>>
>>
>>
>> ----------------------------------------- Confidentiality Notice:
>> The following mail message, including any attachments, is for the
>> sole use of the intended recipient(s) and may contain confidential
>> and privileged information. The recipient is responsible to
>> maintain the confidentiality of this information and to use the
>> information only for authorized purposes. If you are not the
>> intended recipient (or authorized to receive information for the
>> intended recipient), you are hereby notified that any review, use,
>> disclosure, distribution, copying, printing, or action taken in
>> reliance on the contents of this e-mail is strictly prohibited. If
>> you have received this communication in error, please notify us
>> immediately by reply e-mail and destroy all copies of the original
>> message. Thank you.
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 17
> Date: Wed, 30 Nov 2011 17:55:14 +0000
> From: "Martin, Erin" 
> Subject: [Histonet] ASR FISH probes
> To: histonet 
> Message-ID: <24B7B291CC88D04AB663958E77A1F59D0133E7@ex09.net.ucsf.edu>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi all,
>
>
>
> More questions on FISH.  How does one go about validating an ASR probe on
> FFPE tissue?  I have no FISH experience and therefore am starting from
> scratch.  The specific probes that our pathologists are interested in are:
>  t(14;18), t(17;22) and t(2;5).
>
>
>
> Also, does anyone know what educational and training background is
> required to be able to do such a workup?  I know IVDs are no problems, I
> looked at the CAP checklist for molecular and it seems that this is
> usually done in cytogenetics and the tech needs to at least have a BS and
> experience with molecular.
>
>
>
> Any help would be greatly appreciated!
>
>
>
> Thanks,
>
> Erin
>
>
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 96, Issue 42
> ****************************************
>



From liz <@t> premierlab.com  Wed Nov 30 17:33:52 2011
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Wed Nov 30 17:33:57 2011
Subject: [Histonet] RE: ASCP membership
In-Reply-To: <81487B04-08E0-4A91-92A9-5B40A63BA812@mimectl>
Message-ID: <14E2C6176416974295479C64A11CB9AE011380AC71B3@SBS2K8.premierlab.local>

I think it is benefital, I believe you have access to 3 CEU credits each year when you join. I would also look at the Remember program too.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana Martinez-Longoria
Sent: Wednesday, November 30, 2011 1:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ASCP membership

Hello all,



I was wondering if the ASCP membership is worth enrolling, especially since it is a little pricey but it can also keep track with regards too CMP's. Your input would be very beneficial. Thank you ahead for your time.



Diana Martinez-Longoria

Histotechnician (ASCP)cm

El Centro Regional Medical Center

phone: 760-339-7267

fax: 760-482-5365

email:dmlongoria@ecrmc.org




ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.
?


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From pruegg <@t> ihctech.net  Wed Nov 30 17:37:20 2011
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Wed Nov 30 17:37:19 2011
Subject: [Histonet] FW: EM questions
Message-ID: <38EBF84C289C41E8A822243B6C5FF109@Patsyoffice>

Can anyone help with this, I haven't got a clue?

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg@ihctech.net
web site www.ihctech.net
 

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.


-----Original Message-----
From: Suarez, Andrea Luisa [mailto:ANDREA.SUAREZ@UCDENVER.EDU] 
Sent: Wednesday, November 30, 2011 3:22 PM
To: pruegg@ihctech.net
Cc: High, Whitney
Subject: EM questions

Hi Patsy,

Not sure if you remember me from my days as a graduate student in the van
Dyk lab.  How are you?  
I am working w/ Dr Whit High at CU dermpath on an EM project.  I had some
questions that perhaps you might have some answers to.  We are doing a
project where we will be doing QEMSCAN at school of mines, which has the
capacity to do a very sophisticated analysis of mineral composition.  We
will be looking for volcanic ash deposition in human skin samples, and would
like to embedd the tissues in as pure a fashion as possible, with optimum
antigen preservation.  We have some concerns about parrafin embedding, as we
plan to mount the sections onto carbon planchets.  Our experience w/ this in
the past, is that adherence to the planchet is poor after deparafinizaiton.
Therefore, we would like to optimize adherence, if possible, or avoid the
need for deparafinization by embedding in a wax (such as carnuba) w/ a very
high melting temp.

1.) Do you have any experience/insight regarding embedding in carnuba wax?
Do you know of anyone who would be willing to do this for us?
2.) Do you have any recomendations as to how to improve adherence to the
carbon planchet in the event that we do end up having to de-parafinize?  We
were thinking that adding albumin to the water bath may help things.  Any
thoughts on this?


Many thanks and I look forward to hearing from you.

Sincerely,

Andrea Suarez, MD/PhD
Colorado Health Foundation Transitional Intern
andrea.suarez@ucdenver.edu