[Histonet] Americlear Staining Problems

Stella Mireles estellamireles <@t> gmail.com
Thu May 5 07:49:57 CDT 2011


Good Morning Histonetters,
Our H&E stained slides are bleeding out the eosin. No, I did not shoot the
sheriff.
Called the manufacturer, and was advised to: Give more time in the Alcohols,
Use only 100% Alcohols after eosin. Change the last two Alcohols and
Americlear. Done. Done. Done.
Stain bleeding really improved, but still shows up on hit and miss cases.
Has anyone had this problem and How did you fix it

Stella


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