From rjbuesa <@t> yahoo.com Sun May 1 10:26:28 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun May 1 10:26:32 2011 Subject: [Histonet]Need input: Ink dissolved from Cassettes during processing. In-Reply-To: <1025397262-1304208760-cardhu_decombobulator_blackberry.rim.net-906243174-@bda2889.bisx.prod.on.blackberry> Message-ID: <218174.11159.qm@web65703.mail.ac4.yahoo.com> I think taking a photo is problematic because it needs to be a photo with enough resolution to "catch" all the numbers, time consuming and an "over kill". As to a paper inside the cassette FOR SURE it will interfere with the flow of reagents and you will be having to deal with "unprocessed" tissues frequently. The best way is a log as descriptive as possible (as I used to do). Ren? J. --- On Sat, 4/30/11, kiran_g@sbcglobal.net wrote: From: kiran_g@sbcglobal.net Subject: Re: [Histonet]Need input: Ink dissolved from Cassettes during processing. To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu Date: Saturday, April 30, 2011, 8:12 PM Thank you! Does anybody take a digital photo to correlate cassettes when using hand written cassettes as a back up? Or Do you put a piece of paper inside the cassette as back up for hand written cassettes? Need input so we can prevent future incidents. Thx Kiranjit Sent from my Verizon Wireless BlackBerry From: Rene J Buesa Date: Sat, 30 Apr 2011 07:39:14 -0700 (PDT) To: ; Kiranjit Grewal Subject: Re: [Histonet] No patient ID: Ink dissolved from Cassettes during processing. Eleven years ago that happened in our laboratory in what we started to call "The Black Tuesday" (it was from Monday to Tuesday). Thanks that we always kept all cassettes described-written in a log with the type of tissue and the number of pieces with their sizes. Then we went through the list of cassettes that were stored in the baskets in sequential order. That, and the cases description, allowed us to identify all the 268 cassettes. We also informed the chief pathologist and documented it in our QC. The HT that "decided" to use a different pencil to write the cassettes was counseled. We also instituted a check in of the pencil before writing the daily cassette load. I sympathize with your issue, it was really a nightmare in our lab that we were fortunate enough to overcome (thanks to our chain of custody procedure). Ren? J. --- On Fri, 4/29/11, Kiranjit Grewal wrote: From: Kiranjit Grewal Subject: [Histonet] No patient ID: Ink dissolved from Cassettes during processing. To: histonet@lists.utsouthwestern.edu Date: Friday, April 29, 2011, 5:43 PM Hi All, ? What is the standard practice out in histology world?if?hand written cassette id washed?away during processing? ? Please share if you had any experience and how did you resolve this and what is your current practice. ? ? Thank you so much! ? -Kiranjit ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From poupakfar <@t> yahoo.com Sun May 1 13:02:56 2011 From: poupakfar <@t> yahoo.com (Poupak Farahani) Date: Sun May 1 13:03:07 2011 Subject: [Histonet] Re: Histonet Digest, Vol 89, Issue 15 Message-ID: <0E44458E-FB25-4721-A32E-C5643077B747@yahoo.com> Pkikkkkkkkkkkkkkkkk Sent from my iPad On Apr 18, 2011, at 7:43 AM, histonet-request@lists.utsouthwestern.edu wrote: Woeful LNG > Send Histonet mailing list submissions to > histonet@lists.utsouthwestew > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > U > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Tissue transfer (Bartlett, Jeanine (CDC/OID/NCEZID)) > 2. General Data Cassette Labeler (Robin Negron) > 3. RE: How long should autopsy/necropsy tissue be in fixation > for a suspected TB case? (Thurby, Christina) > 4. Re: Tissue transfer (Diane.Craft@amcny.org) > 5. Re: RE: How long should autopsy/necropsy tissue be in > fixation for a suspected TB case? (Jay Lundgren) > 6. Re: slide brite and coverslip drying time (Rene J Buesa) > 7. Re: RE: How long should autopsy/necropsy tissue be in > fixation for a suspected TB case? (Rene J Buesa) > 8. DAB staining for Liver frozen sections (Nasreen Bashir) > 9. RE: RE: How long should autopsy/necropsy tissue be in > fixation for a suspected TB case? (Kumar, Devender) > 10. Re: Tissue transfer (Rene J Buesa) > 11. Part-time Histotech Opening (Norm Burnham) > 12. Re: Update regarding question on Plastic Embedding (Joseph Saby) > 13. Seeking techs all over the place! (Cheryl) > 14. Histology Supervisor ( Hospital Scientist)-F/T (Fawaz Zouabi) > 15. RE: RE: How long should autopsy/necropsy tissue be in > fixation for a suspected TB case? (Tony Henwood) > 16. RE: Tissue transfer (Kuhnla, Melissa) > 17. Cassette labeling (Giracello, Darlene) > 18. AKT anyone? (Amos Brooks) > 19. [Help] oil red O stain in fattty change liver (kyoung LEE) > 20. RE: [Help] oil red O stain in fattty change liver (Setlak, Lisa) > 21. need monkey control tissue (Jan Shivers) > 22. Activated Carbon (Travis Troyer) > 23. Re: [Help] oil red O stain in fatty change liver > (Jennifer MacDonald) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 13 Apr 2011 17:06:59 +0000 > From: "Bartlett, Jeanine (CDC/OID/NCEZID)" > Subject: [Histonet] Tissue transfer > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing. Any suggestions? > > Thanks! > > Jeanine Bartlett, BS, HT(ASCP)QIHC > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > > > > ------------------------------ > > Message: 2 > Date: Wed, 13 Apr 2011 13:32:57 -0400 > From: "Robin Negron" > Subject: [Histonet] General Data Cassette Labeler > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > > Can anyone that uses the General Data cassette labeler > give me information regarding your overall opinion of this instrument. > > > > > > 1. I would like to have any information regarding the speed of > machine. When we did a demo of this equipment it seems fast. > > 2. I was also questioning the cassettes, we have found that the > only company to purchase these from is general data (at a high cost). > > 3. Has anyone had the equipment break down and how is the > dependability and the customer support service. > > 4. Our company is very interested in the on demand version. How > has anyone found this to be an advantage. > > 5. Does anyone purchase the cassettes from another dealer besides > General Data > > 6. Has anyone had problems with the black scatching off. > > 7. We currently use TBS and they are very slow and constantly > break down this does not meet our demand as we currently print approx. > 400-500 per day. > > > > > > I would like to hear your opinions. > > > > ------------------------------ > > Message: 3 > Date: Wed, 13 Apr 2011 14:04:56 -0400 > From: "Thurby, Christina" > Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in > fixation for a suspected TB case? > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Hi, > Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. > Thanks, > Christina Thurby > Bristol-Myers Squibb Company > Research Scientist I > 812-307-2093 (tie 625) > > > > > This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. > > > > ------------------------------ > > Message: 4 > Date: Wed, 13 Apr 2011 14:11:38 -0400 > From: Diane.Craft@amcny.org > Subject: Re: [Histonet] Tissue transfer > To: "Bartlett, Jeanine (CDC/OID/NCEZID)" > Cc: "histonet@lists.utsouthwestern.edu" > , > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > I use Harleco Krystalon liquid coverslip medium. Cover the tissue, laying > horizontally in oven for a few hours until it hardens, than soak in water > bath, peel, and transfer to charged slide with same side facing down, dry > in oven again, dissolve in xylene when ready for staining. > > Diane Craft > Pathology Department > Animal Medical Center > 510 East 62nd St > New York NY 10065-8314 > 212-329-8675 (phone) > 212-759-5878 (fax) > > > > "Bartlett, Jeanine (CDC/OID/NCEZID)" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 04/13/2011 01:06 PM > > To > "histonet@lists.utsouthwestern.edu" > cc > > Subject > [Histonet] Tissue transfer > > > > > > Hello all, > > We have some unstained slides we received from outside the U.S. on > non-charged/un-coated slides. We would like to transfer these sections to > charged slides before proceeding with testing. Any suggestions? > > Thanks! > > Jeanine Bartlett, BS, HT(ASCP)QIHC > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 5 > Date: Wed, 13 Apr 2011 14:31:09 -0500 > From: Jay Lundgren > Subject: Re: [Histonet] RE: How long should autopsy/necropsy tissue be > in fixation for a suspected TB case? > To: "Thurby, Christina" > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset=ISO-8859-1 > > As long as possible. I remember reading somewhere about TB in lung > tissue that was still viable after *years* of 10% NBF. Make sure the lungs > are thoroughly perfused, or they will still be unfixed in the middle. There > is a technique to "inflate" lungs at autopsy using a large syringe full of > NBF and a clamp. Make sure that the specimen is not contacting the walls or > bottom of the specimen container. You can suspend the whole organ with > string inside the container, if needed. One can also use gauze or paper > towels to keep the organ under the surface of the fixative. Lung tissue is > notoriously difficult to fix properly, due both to the high lipid content of > the pleural parenchyma, and the fact that fresh lungs float. > > Sincerely, > > Jay A. > Lundgren, M.S., HTL (ASCP) > > > ------------------------------ > > Message: 6 > Date: Wed, 13 Apr 2011 12:51:07 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] slide brite and coverslip drying time > To: Histonet@lists.utsouthwestern.edu, Dawn Herron > > Message-ID: <237269.18418.qm@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > That is exactly one of the problems with Slide Brite, and is the "trade-off" you have to pay. > The alternative (although it may seem "odd") is to get away with alcohols and xylene altogether. > After staining the slides, wash them in distilled water, shake them well and place them in an over at 60?C for 5 minutes. After drying apply the mounting medium more diluted (consistency of light mineral oil), and coverslip. > You will get away with alcohols, and xylene (or its substitutes). > Do not "frown", just try it! > Ren? J. > > --- On Wed, 4/13/11, Dawn Herron wrote: > > > From: Dawn Herron > Subject: [Histonet] slide brite and coverslip drying time > To: Histonet@lists.utsouthwestern.edu > Date: Wednesday, April 13, 2011, 12:16 PM > > > Hello all. We have just made the transition from xylene in our linear > stainer to Slide Brite. We are also coverslipping from Slide Brite with > Permount. So far the slides look good (though we have to be careful about > water contamination in the dehydrating alcohols) but we are having a problem > with extremely long coverslipping drying time. Some of the slides have been > on the warmer for over 18 hours and you can still move the coverslips! > (Normally the slides would dry within 4 hours on the warmer with xylene and > acrymount) Any suggestions on how to speed drying time? > > Thanks, > Dawn > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 7 > Date: Wed, 13 Apr 2011 12:57:28 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] RE: How long should autopsy/necropsy tissue be > in fixation for a suspected TB case? > To: "histonet@lists.utsouthwestern.edu" > , ChristinaThurby > > Message-ID: <632902.43444.qm@web65712.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > 5 days (96 h) is more than enough, but always handle the tissue and blocks using safety precautions. > Ren? J. > > --- On Wed, 4/13/11, Thurby, Christina wrote: > > > From: Thurby, Christina > Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? > To: "histonet@lists.utsouthwestern.edu" > Date: Wednesday, April 13, 2011, 2:04 PM > > > Hi, > Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. > Thanks, > Christina Thurby > Bristol-Myers Squibb Company > Research Scientist I > 812-307-2093 (tie 625) > > > > > This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 8 > Date: Wed, 13 Apr 2011 16:07:43 -0400 > From: Nasreen Bashir > Subject: [Histonet] DAB staining for Liver frozen sections > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=ISO-8859-1 > > Hi, > I need some information on how to block endogenous peroxidase effectively > in liver frozen sections, as I need to do DAB staining. > What blocking buffer will work best for blocking in liver frozen > sections. > I appreciate any information on this issue. > > Thanks, > Reena > > > ------------------------------ > > Message: 9 > Date: Wed, 13 Apr 2011 16:08:33 -0400 > From: "Kumar, Devender" > Subject: RE: [Histonet] RE: How long should autopsy/necropsy tissue be > in fixation for a suspected TB case? > To: Rene J Buesa , > "histonet@lists.utsouthwestern.edu" > , ChristinaThurby > > Message-ID: > > > Content-Type: text/plain; charset="iso-8859-1" > > Hi, > > We fix 'Category-A' agent infected murine lungs in 10% buffered formalin for 7 days in ABSL-3. We also confirm tissue sterility by plating the tissue homogenates on suitable media before taking the samples out of ABSL-3 facility for sectioning. In your case 7 days should be enough, but it is always better to confirm the sterility of tissue. > > Thanks, > Devender > > > Devender Kumar, D.V.M., Ph.D. > Postdoctorate fellow, > Center for Immunology & Microbial Disease, > Albany Medical College, > 47 New Scotland Avenue, MC 151 > Albany, NY 12208 > 518.262.6220(LAB) > -- > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Wednesday, April 13, 2011 3:57 PM > To: histonet@lists.utsouthwestern.edu; ChristinaThurby > Subject: Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? > > 5 days (96 h) is more than enough, but always handle the tissue and blocks using safety precautions. > Ren? J. > > --- On Wed, 4/13/11, Thurby, Christina wrote: > > > From: Thurby, Christina > Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? > To: "histonet@lists.utsouthwestern.edu" > Date: Wednesday, April 13, 2011, 2:04 PM > > > Hi, > Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. > Thanks, > Christina Thurby > Bristol-Myers Squibb Company > Research Scientist I > 812-307-2093 (tie 625) > > > > > This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ----------------------------------------- > CONFIDENTIALITY NOTICE: This email and any attachments may contain > confidential information that is protected by law and is for the > sole use of the individuals or entities to which it is addressed. > If you are not the intended recipient, please notify the sender by > replying to this email and destroying all copies of the > communication and attachments. Further use, disclosure, copying, > distribution of, or reliance upon the contents of this email and > attachments is strictly prohibited. To contact Albany Medical > Center, or for a copy of our privacy practices, please visit us on > the Internet at www.amc.edu. > > > > ------------------------------ > > Message: 10 > Date: Wed, 13 Apr 2011 12:54:12 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Tissue transfer > To: "histonet@lists.utsouthwestern.edu" > , " Jeanine > \(CDC/OID/NCEZID\)Bartlett" > Message-ID: <899879.15372.qm@web65713.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > "Mission impossible" (successfully, you can always ruin them)! > It is better to do the staining after a prolonged oven heating and do it delicately. > Ren? J. > > --- On Wed, 4/13/11, Bartlett, Jeanine (CDC/OID/NCEZID) wrote: > > > From: Bartlett, Jeanine (CDC/OID/NCEZID) > Subject: [Histonet] Tissue transfer > To: "histonet@lists.utsouthwestern.edu" > Date: Wednesday, April 13, 2011, 1:06 PM > > > Hello all, > > We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing. Any suggestions? > > Thanks! > > Jeanine Bartlett, BS, HT(ASCP)QIHC > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 11 > Date: Wed, 13 Apr 2011 16:52:58 -0500 > From: "Norm Burnham" > Subject: [Histonet] Part-time Histotech Opening > To: > Cc: Angie.Viancourt@propath.com > Message-ID: > <82C7248978CB50469FD6BA68EBBEFE6705653652@exchange.propathlab.com> > Content-Type: text/plain; charset="us-ascii" > > Dear Histonetters, > > > > We have the following opening: > > > > > > HISTOTECHNOLOGIST > > > > ProPath, a high volume, pathology practice, located in Dallas, Texas, has an > immediate opening for a part-time Histotechnologist. Responsibilities > include embedding tissue specimens, microtomy of paraffin-embedded tissue, > operation of automated stainer and coverslipper, equipment maintenance and > record retention. > > > > The ideal candidate will have a high school diploma or equivalent. We > prefer, HT, HTL (ASCP) registered or eligible. > > > > The hours are 2:00 p.m. to 6:00 p.m. Monday - Friday. > > > > For consideration send resume to: > > ProPath, Human Resources > > 1355 River Bend Drive > > Dallas, TX 75247 > > FAX: 214/237-1825 > > Job-Line: 214/237-1775 > > Email address: jobs@propath.com > > Website: www.propath.com > > > > Don't Follow the Leader! Join the Leader! > > > > EOE > > > > > > > > ------------------------------ > > Message: 12 > Date: Wed, 13 Apr 2011 15:51:41 -0700 (PDT) > From: Joseph Saby > Subject: Re: [Histonet] Update regarding question on Plastic Embedding > To: Mahesh Polavarapu , histonet > > Message-ID: <219317.4678.qm@web114401.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Mahesh- > > If you have access to a sonicator, you can etch the slides by first soaking them > in 1% formic acid in the sonicator for 5-10 minutes, then after a brief water > rinse soak them in 50% ethanol for another 5-10 minutes in the sonicator. > > You may have to work with the staining times, but you will find that many of > your paraffin embedding stains will work. > > Good luck! > > Joe Saby, BA HT > NAMSA > > > > ________________________________ > From: Mahesh Polavarapu > To: histonet > Sent: Tue, April 12, 2011 8:25:30 PM > Subject: [Histonet] Update regarding question on Plastic Embedding > > Looking for a protocol to visualize vascularization and collagen deposition > at the bone-tendon interface of a rabbit rotator cuff embedded in a plastic > system. Sections will be rather thick (~50um) b/c they are being made > through a titanium anchor. Using MMA with a cold-curing resin, Technovit > 9100. Thanks in advance! > > - Mahesh > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 13 > Date: Wed, 13 Apr 2011 18:04:50 -0700 (PDT) > From: Cheryl > Subject: [Histonet] Seeking techs all over the place! > To: histonet@lists.utsouthwestern.edu > Message-ID: <547837.58901.qm@web39422.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > > Help! > > We're getting busy again (the economy rebounds??) and need both permanent and temporary histotechs. > > Are you looking for... > ...a new place to live? > ...a bigger city? > ...a smaller city? > ...a promotion? > ...a raise? > ...a place to learn new things? > > We will actively consider... > ...new grads (and almost grads) > ...experienced bench techs > ...bench techs looking for a step up > ...experienced management > ...retirees (we know you've still 'got it') > > We have a number of permanent openings and seeking to successfully fill 9 thirteen-week temp positions. We are working histotechs so the conversation is easy and usually kinda fun. > Send a resume or ask for an application - > tkngflght@yahoo.com > Fax/Phone: 800.756.3309 > > ------------------------------ > > Message: 14 > Date: Thu, 14 Apr 2011 13:44:54 +1000 > From: "Fawaz Zouabi" > Subject: [Histonet] Histology Supervisor ( Hospital Scientist)-F/T > To: > Message-ID: > > > Content-Type: text/plain; charset="iso-8859-1" > > A Histology Supervisor ( Hospital Scientist) -F/T position is currently available at The Department of Forensic Medicine (Sydney local health network / Sydney south West), GLEBE 2037 NSW AUSTRALIA. > To enquire please check the NSW health web site on http://www.health.nsw.gov.au/jobs/index.asp > The successful applicant will be responsible of the supervision of the DOFM histology laboratory which involves the collection and processing of forensic histological specimens. > > > > > ------------------------------ > > Message: 15 > Date: Thu, 14 Apr 2011 06:58:57 +0000 > From: Tony Henwood > Subject: RE: [Histonet] RE: How long should autopsy/necropsy tissue be > in fixation for a suspected TB case? > To: "'Thurby, Christina'" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: <6D6BD1DE8A5571489398B392A38A71571884F5E6@xmdb02.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Christina, > > The following might be useful: > > Kappel et al (HUM PATHOL 27:1361--1364, 1996) attempted to grow TB from formalin fixed lung tissue that had previously been shown to be positive by sputum culture. They were unable to culture TB from these tissues. > > Gerston et al (HUM PATHOL 35:571-575.2004) in South Africa analysed 138 formalin fixed lungs with histological evidence of AFB and were able to culture TB from 12 of these cases (one of these cases had been fixed for 80 days before being tested). > > Gerston et al suggest that there is a risk of contracting tuberculosis from tissue that has been fixed in formalin, if aerosols or accidental inoculation should occur. > > Trimming and sectioning wax blocks are of concern but no studies have been done yet. > > Of concern to histotechnolgists are: > 1. Tissue with Inflammation-induced Encapsulation may protect bugs from formalin. > 2. Formalin dilutes as it penetrates tissue. > 3. Formalin substitutes may not be germicidal. > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thurby, Christina > Sent: Thursday, 14 April 2011 4:05 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? > > Hi, > Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. > Thanks, > Christina Thurby > Bristol-Myers Squibb Company > Research Scientist I > 812-307-2093 (tie 625) > > > > > This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > > > ------------------------------ > > Message: 16 > Date: Thu, 14 Apr 2011 07:11:02 -0400 > From: "Kuhnla, Melissa" > Subject: RE: [Histonet] Tissue transfer > To: Rene J Buesa , > , " Jeanine > (CDC/OID/NCEZID)Bartlett" > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Hello, > We do have a successful way to transfer tissue sections. It is using Mount Quik liquid coverslip. We purchase this from Newcomber Supply. Are you doing IHC testing? > 1. H&E sections first (so they are visible)(for IHC, retrieval will fade the stain anyways) > 2. After the stain, run slide to Xylene > 3. Cover entire slide with layer of Mount Quik > 4. Bake horizontal in 60 degree oven for 1 hour > 5. Bake vertical for one hour > 6. Rinse slide in running warm water until the mount quik and tissue lift from the slide > 7. Divide the tissue as need, place portions on plus slide > 8. Bake horizontal for 1 hour > 9. Bake vertical for one hour > 10. Dissolve off mount quik with several 10 minute steps in Xylene. No visible residual should be left > 11. Carry out testing > > Hope this helps > > Melissa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Wednesday, April 13, 2011 3:54 PM > To: histonet@lists.utsouthwestern.edu; Jeanine (CDC/OID/NCEZID)Bartlett > Subject: Re: [Histonet] Tissue transfer > > "Mission impossible" (successfully, you can always ruin them)! > It is better to do the staining after a prolonged oven heating and do it delicately. > Ren? J. > > --- On Wed, 4/13/11, Bartlett, Jeanine (CDC/OID/NCEZID) wrote: > > > From: Bartlett, Jeanine (CDC/OID/NCEZID) > Subject: [Histonet] Tissue transfer > To: "histonet@lists.utsouthwestern.edu" > Date: Wednesday, April 13, 2011, 1:06 PM > > > Hello all, > > We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing. Any suggestions? > > Thanks! > > Jeanine Bartlett, BS, HT(ASCP)QIHC > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. > > > > > > ------------------------------ > > Message: 17 > Date: Thu, 14 Apr 2011 09:19:00 -0500 > From: "Giracello, Darlene" > Subject: [Histonet] Cassette labeling > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <0C2BDF59DC80E64AB2A59939D703B2E5016BD5C2EEC1@STWEXE3.ad.okstate.edu> > Content-Type: text/plain; charset="iso-8859-1" > > > Our cassette printer finally gave up on us, and we are looking at available options. > > Does anyone have experience with the Brady cassette labeling system? We would really appreciate ANY feedback about this product. We are also open to suggestions. We are a veterinary lab with budget constraints :-) > > Thank you in advance for your help > Darlene > > > ------------------------------ > > Message: 18 > Date: Thu, 14 Apr 2011 10:25:16 -0400 > From: Amos Brooks > Subject: [Histonet] AKT anyone? > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=ISO-8859-1 > > Hi, > I am trying to get AKT working on formalin fixed paraffin embedded human > surgical pathology tissue. I have tried two vendors (Cell Signalling and > Epitomics) and I'm having terrible difficulty getting anything out of it. I > was wondering if anyone else is using AKT (from anywhere) and is getting > decent results. The PI here is getting really upset about it and I feel like > I've done everything but stand on my head while doing the test. > > Thanks, > Amos > > > ------------------------------ > > Message: 19 > Date: Thu, 14 Apr 2011 23:07:24 +0900 (KST) > From: kyoung LEE > Subject: [Histonet] [Help] oil red O stain in fattty change liver > To: histonet@lists.utsouthwestern.edu > Message-ID: <147665.31524.qm@web70905.mail.kr3.yahoo.com> > Content-Type: text/plain; charset=utf-8 > > Dear sirs. > > I don't know whether I send this question to you. > > For several weeks, I'm setting oil red O stain in fattty change liver (mouse). > > Could you review my protocol? > > 1. Murine tissue,livers, is fixed with 10% NBF(10% neutral-buffered formalin). > 2. Make frozen block > 3. Blocks were cut 5um in crystat and dried for 1~2H at RT.? > 4. store at -20 > 5. let it dry for 30mins at RT > 6. immerse it in cold 10% NBF for 10mins > 7. staining > > After staining, my section were fallen? off and seperated. > > I search many protocols. Some suggest additional fixation is necessary as soon > as cutting. > Others suggest? prefixed tissue is not? necessary additional fixation. > > Plz send me your comments. > > ------------------------------ > > Message: 20 > Date: Thu, 14 Apr 2011 10:09:41 -0500 > From: "Setlak, Lisa" > Subject: RE: [Histonet] [Help] oil red O stain in fattty change liver > To: 'kyoung LEE' , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <7111DB39D045004C9CF29E79C71B28BC101D50791C@CMHEXCC01MBX.childrensmemorial.org> > > Content-Type: text/plain; charset="iso-8859-1" > > We do this stain on frozen sections in our lab with no formalin fixation. We stain in oil red o from American Mastertech for 30 minutes, rinse in water, counterstain in hematoxylin, coverslip with aqueous mounting media. > Lisa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kyoung LEE > Sent: Thursday, April 14, 2011 9:07 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] [Help] oil red O stain in fattty change liver > > Dear sirs. > > I don't know whether I send this question to you. > > For several weeks, I'm setting oil red O stain in fattty change liver (mouse). > > Could you review my protocol? > > 1. Murine tissue,livers, is fixed with 10% NBF(10% neutral-buffered formalin). > 2. Make frozen block > 3. Blocks were cut 5um in crystat and dried for 1~2H at RT. > 4. store at -20 > 5. let it dry for 30mins at RT > 6. immerse it in cold 10% NBF for 10mins > 7. staining > > After staining, my section were fallen off and seperated. > > I search many protocols. Some suggest additional fixation is necessary as soon > as cutting. > Others suggest prefixed tissue is not necessary additional fixation. > > Plz send me your comments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 21 > Date: Thu, 14 Apr 2011 10:56:11 -0500 > From: "Jan Shivers" > Subject: [Histonet] need monkey control tissue > To: "histonet" > Message-ID: <0B3AF0D33B2447EF910223B1D49D1226@auxs.umn.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Hello, > I'm posting this request for a colleague, who is in need of normal monkey placenta (preferably Rhesus). If anyone has some to spare, please let me know and I'll forward your response. > > Thanks, > Jan Shivers > Senior Scientist > Histology/IHC/EM Section Head > Pathology Teaching Program > University of Minnesota > Veterinary Diagnostic Laboratory > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > > (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) > > > ------------------------------ > > Message: 22 > Date: Thu, 14 Apr 2011 11:08:21 -0500 > From: "Travis Troyer" > Subject: [Histonet] Activated Carbon > To: > Message-ID: <367C74D495C248669E61229AFF3B60EB@Peterson.local> > Content-Type: text/plain; charset="iso-8859-1" > > Does anyone have an inexpensive vendor for activated carbon for the processor? > > Thanks, > Travis > > ------------------------------ > > Message: 23 > Date: Thu, 14 Apr 2011 09:10:33 -0700 > From: Jennifer MacDonald > Subject: Re: [Histonet] [Help] oil red O stain in fatty change liver > To: kyoung LEE > Cc: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > We use pre-fixed tissue for oil red o. We rinse the section briefly to > remove the formalin from the outside of the tissue. We prepare our tissue > in OCT or the equivalent. We cut the frozen sections and mount them on > charged slides. We let dry the dry a minimum of 10 minute, rinse them in > water to remove the OCT and then proceed with the stain. > > > > > kyoung LEE > Sent by: histonet-bounces@lists.utsouthwestern.edu > 04/14/2011 08:04 AM > > To > histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] [Help] oil red O stain in fattty change liver > > > > > > > Dear sirs. > > I don't know whether I send this question to you. > > For several weeks, I'm setting oil red O stain in fattty change liver > (mouse). > > Could you review my protocol? > > 1. Murine tissue,livers, is fixed with 10% NBF(10% neutral-buffered > formalin). > 2. Make frozen block > 3. Blocks were cut 5um in crystat and dried for 1~2H at RT. > 4. store at -20 > 5. let it dry for 30mins at RT > 6. immerse it in cold 10% NBF for 10mins > 7. staining > > After staining, my section were fallen off and seperated. > > I search many protocols. Some suggest additional fixation is necessary as > soon > as cutting. > Others suggest prefixed tissue is not necessary additional fixation. > > Plz send me your comments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 89, Issue 15 > **************************************** From amitapandey <@t> torrentpharma.com Sun May 1 23:04:23 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Sun May 1 23:04:36 2011 Subject: [Histonet]Need input: Ink dissolved from Cassettes during processing. In-Reply-To: <1025397262-1304208760-cardhu_decombobulator_blackberry.rim.net-906243174-@bda2889.bisx.prod.on.blackberry> References: <45567.85076.qm@web180103.mail.gq1.yahoo.com><556688.58493.qm@web65715.mail.ac4.yahoo.com> <1025397262-1304208760-cardhu_decombobulator_blackberry.rim.net-906243174-@bda2889.bisx.prod.on.blackberry> Message-ID: We write the sample detail on a small piece of paper using pencil not the pen. Also we keep the record on formal how many cassettes kept for processing and what were the samples? This help us to track back by chance if loose the detail. Amita From: kiran_g@sbcglobal.net To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu Date: 01/05/11 05:42 AM Subject: Re: [Histonet]Need input: Ink dissolved from Cassettes during processing. Sent by: histonet-bounces@lists.utsouthwestern.edu Thank you! Does anybody take a digital photo to correlate cassettes when using hand written cassettes as a back up? Or Do you put a piece of paper inside the cassette as back up for hand written cassettes? Need input so we can prevent future incidents. Thx Kiranjit Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Date: Sat, 30 Apr 2011 07:39:14 To: ; Kiranjit Grewal Subject: Re: [Histonet] No patient ID: Ink dissolved from Cassettes during processing. Eleven years ago that happened in our laboratory in what we started to call "The Black Tuesday" (it was from Monday to Tuesday). Thanks that we always kept all cassettes described-written in a log with the type of tissue and the number of pieces with their sizes. Then we went through the list of cassettes that were stored in the baskets in sequential order. That, and the cases description, allowed us to identify all the 268 cassettes. We also informed the chief pathologist and documented it in our QC. The HT that "decided" to use a different pencil to write the cassettes was counseled. We also instituted a check in of the pencil before writing the daily cassette load. I sympathize with your issue, it was really a nightmare in our lab that we were fortunate enough to overcome (thanks to our chain of custody procedure). Ren? J. --- On Fri, 4/29/11, Kiranjit Grewal wrote: From: Kiranjit Grewal Subject: [Histonet] No patient ID: Ink dissolved from Cassettes during processing. To: histonet@lists.utsouthwestern.edu Date: Friday, April 29, 2011, 5:43 PM Hi All, What is the standard practice out in histology world if hand written cassette id washed away during processing? Please share if you had any experience and how did you resolve this and what is your current practice. Thank you so much! -Kiranjit _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Mon May 2 05:06:02 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon May 2 05:06:09 2011 Subject: [Histonet] Histology Waste in Florida In-Reply-To: <137942.53747.qm@web65715.mail.ac4.yahoo.com> References: <000301cc06b9$6a470f20$3ed52d60$@imagesbyhopper.com> <137942.53747.qm@web65715.mail.ac4.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DEBE7C10@LRGHEXVS1.practice.lrgh.org> Thought it was a EPA offense to pour flammables down the drain, especially anything that contains methanol. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, April 30, 2011 10:41 AM To: histonet@lists.utsouthwestern.edu; histotech@imagesbyhopper.com Subject: Re: [Histonet] Histology Waste in Florida Formalin ? towed away?by a safety contractor. Alcohol ? down the drain (recycling alcohol is too costly and time consuming). Ren? J. --- On Fri, 4/29/11, histotech@imagesbyhopper.com wrote: From: histotech@imagesbyhopper.com Subject: [Histonet] Histology Waste in Florida To: histonet@lists.utsouthwestern.edu Date: Friday, April 29, 2011, 6:04 PM Hi Histonetters, This is a question specific to Florida.? Can you share with me what your facilities do with regards to the disposal of formalin and alcohol?? With regards to formalin, do you recycle, haul it away, neutralize etc?? With regards to alcohol, do you haul away, pour down the drain, recycle etc? Does anyone have any specific regs that could help answer these questions? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From micropathlabs <@t> yahoo.com Mon May 2 06:10:15 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Mon May 2 06:10:18 2011 Subject: [Histonet] Histology Waste in Florida In-Reply-To: <000301cc06b9$6a470f20$3ed52d60$@imagesbyhopper.com> References: <000301cc06b9$6a470f20$3ed52d60$@imagesbyhopper.com> Message-ID: <507577.47940.qm@web161711.mail.bf1.yahoo.com> We have?our xylene, alcohol and formalin all hauled away. Where?you? and the quantity are may?dictate how to handle the alcohol and formalin, every city/county are different. Be sure you get the information in writing if you get someone of authority to tell you it's okay to pour down the drain. Hope this helps. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ? ________________________________ From: "histotech@imagesbyhopper.com" To: histonet@lists.utsouthwestern.edu Sent: Fri, April 29, 2011 6:04:25 PM Subject: [Histonet] Histology Waste in Florida Hi Histonetters, This is a question specific to Florida.? Can you share with me what your facilities do with regards to the disposal of formalin and alcohol?? With regards to formalin, do you recycle, haul it away, neutralize etc?? With regards to alcohol, do you haul away, pour down the drain, recycle etc? Does anyone have any specific regs that could help answer these questions? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Mon May 2 07:39:18 2011 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon May 2 07:41:42 2011 Subject: [Histonet] Histology Waste in Florida In-Reply-To: <137942.53747.qm@web65715.mail.ac4.yahoo.com> References: <000301cc06b9$6a470f20$3ed52d60$@imagesbyhopper.com>, <137942.53747.qm@web65715.mail.ac4.yahoo.com> Message-ID: <85F2E7DD5D91744D831A66E44D718B9A23B468A5@fhovxchmb7001.ADVENTISTCORP.NET> V2Uga2VlcCBzb2x2ZW50IHdhc3RlIChBbGNvaG9scywgaGVtYXRveHlsaW4sIGVvc2luLCBhbmQg Zm9ybWFsaW4pICBpbiBvbmUgY29uYXRhaW5lciBhbmQgeHlsZW5lIHdhc3RlIGluIGEgc2Vjb25k IGNvbnRhaW5lciB0byBiZSBoYXVsZWQgb2ZmIGJ5IHRoZSBhcHByb3ByaWF0ZSBkaXNwb3NhbCBj b21wYW55LiAgTk9ORSBvZiB0aGlzIGdvZXMgZG93biB0aGUgZHJhaW4uDQoNCl9fX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fX18NCkZyb206IGhpc3RvbmV0LWJvdW5jZXNAbGlz dHMudXRzb3V0aHdlc3Rlcm4uZWR1IFtoaXN0b25ldC1ib3VuY2VzQGxpc3RzLnV0c291dGh3ZXN0 ZXJuLmVkdV0gT24gQmVoYWxmIE9mIFJlbmUgSiBCdWVzYSBbcmpidWVzYUB5YWhvby5jb21dDQpT ZW50OiBTYXR1cmRheSwgQXByaWwgMzAsIDIwMTEgMTA6NDEgQU0NClRvOiBoaXN0b25ldEBsaXN0 cy51dHNvdXRod2VzdGVybi5lZHU7IGhpc3RvdGVjaEBpbWFnZXNieWhvcHBlci5jb20NClN1Ympl Y3Q6IFJlOiBbSGlzdG9uZXRdIEhpc3RvbG9neSBXYXN0ZSBpbiBGbG9yaWRhDQoNCkZvcm1hbGlu IKH6IHRvd2VkIGF3YXkgYnkgYSBzYWZldHkgY29udHJhY3Rvci4NCkFsY29ob2wgofogZG93biB0 aGUgZHJhaW4gKHJlY3ljbGluZyBhbGNvaG9sIGlzIHRvbyBjb3N0bHkgYW5kIHRpbWUgY29uc3Vt aW5nKS4NClJlbqimIEouDQoNCi0tLSBPbiBGcmksIDQvMjkvMTEsIGhpc3RvdGVjaEBpbWFnZXNi eWhvcHBlci5jb20gPGhpc3RvdGVjaEBpbWFnZXNieWhvcHBlci5jb20+IHdyb3RlOg0KDQoNCkZy b206IGhpc3RvdGVjaEBpbWFnZXNieWhvcHBlci5jb20gPGhpc3RvdGVjaEBpbWFnZXNieWhvcHBl ci5jb20+DQpTdWJqZWN0OiBbSGlzdG9uZXRdIEhpc3RvbG9neSBXYXN0ZSBpbiBGbG9yaWRhDQpU bzogaGlzdG9uZXRAbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1DQpEYXRlOiBGcmlkYXksIEFwcmls IDI5LCAyMDExLCA2OjA0IFBNDQoNCg0KSGkgSGlzdG9uZXR0ZXJzLA0KDQoNCg0KVGhpcyBpcyBh IHF1ZXN0aW9uIHNwZWNpZmljIHRvIEZsb3JpZGEuICBDYW4geW91IHNoYXJlIHdpdGggbWUgd2hh dCB5b3VyDQpmYWNpbGl0aWVzIGRvIHdpdGggcmVnYXJkcyB0byB0aGUgZGlzcG9zYWwgb2YgZm9y bWFsaW4gYW5kIGFsY29ob2w/ICBXaXRoDQpyZWdhcmRzIHRvIGZvcm1hbGluLCBkbyB5b3UgcmVj eWNsZSwgaGF1bCBpdCBhd2F5LCBuZXV0cmFsaXplIGV0Yz8gIFdpdGgNCnJlZ2FyZHMgdG8gYWxj b2hvbCwgZG8geW91IGhhdWwgYXdheSwgcG91ciBkb3duIHRoZSBkcmFpbiwgcmVjeWNsZSBldGM/ DQpEb2VzIGFueW9uZSBoYXZlIGFueSBzcGVjaWZpYyByZWdzIHRoYXQgY291bGQgaGVscCBhbnN3 ZXIgdGhlc2UgcXVlc3Rpb25zPw0KDQoNCg0KVGhhbmtzIQ0KDQpfX19fX19fX19fX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fXw0KSGlzdG9uZXQgbWFpbGluZyBsaXN0DQpIaXN0 b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNCmh0dHA6Ly9saXN0cy51dHNvdXRod2VzdGVy bi5lZHUvbWFpbG1hbi9saXN0aW5mby9oaXN0b25ldA0KX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fX18NCkhpc3RvbmV0IG1haWxpbmcgbGlzdA0KSGlzdG9uZXRA bGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1DQpodHRwOi8vbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1 L21haWxtYW4vbGlzdGluZm8vaGlzdG9uZXQ= From flnails <@t> texaschildrens.org Mon May 2 08:54:26 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Mon May 2 08:54:33 2011 Subject: [Histonet] RE: grossing stations In-Reply-To: <24A4826E8EF0964D86BC5317306F58A55DF526DAEF@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A55DF526DAEF@mmc-mail.ad.mhsil.com> Message-ID: We switched from the gross lab senior because of premature rusting, with the Sakura demo our pathologist thought it was too loud. We selected the Mopec MB600 and they have been very pleased. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, April 26, 2011 7:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] grossing stations We are looking into new grossing stations. Can anybody give me their opinions on which to choose, experiences, and what to avoid? 1. Mopec's Grossing Stations 2. ThermoFisher Gross Lab Senior 3. Accu-Edge Workstations by Sakura James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From rjbuesa <@t> yahoo.com Mon May 2 09:42:05 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 2 09:42:08 2011 Subject: [Histonet] Question to all my colleagues Message-ID: <225033.48056.qm@web65716.mail.ac4.yahoo.com> Dear Colleague: I have been told that there is an increased difficulty in filling histechnicians and histotechnologists positions?by some laboratories. My question is: does it take more time now to fill such positions than, lets say, 3 years ago? If that is the case, approximately how much time? I would like to find out if this is an issue mainly related to an specific area of the country. I will really appreciate your answer. Ren? J. From amber.mckenzie <@t> gastrodocs.net Mon May 2 09:48:15 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon May 2 09:49:17 2011 Subject: [Histonet] Question to all my colleagues In-Reply-To: <225033.48056.qm@web65716.mail.ac4.yahoo.com> References: <225033.48056.qm@web65716.mail.ac4.yahoo.com> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B370414AA97@giamail2.Gia.com> In MS, it's a problem of not having anyone to fill the vacant positions. And once you find someone, that person can pretty much say the hours she/he wants and the pay b/c you have no other candidates to choose from. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, May 02, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question to all my colleagues Dear Colleague: I have been told that there is an increased difficulty in filling histechnicians and histotechnologists positions?by some laboratories. My question is: does it take more time now to fill such positions than, lets say, 3 years ago? If that is the case, approximately how much time? I would like to find out if this is an issue mainly related to an specific area of the country. I will really appreciate your answer. Ren? J. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kblack <@t> digestivehlth.com Mon May 2 10:08:39 2011 From: kblack <@t> digestivehlth.com (Konni Black) Date: Mon May 2 10:08:47 2011 Subject: [Histonet] HTL/HT job opening Message-ID: HT/ HTL position in Columbia, SC. This is a fulltime position for a licensed HT/ HTL with minimum 3 to 5 years experience. Flexible schedule and great working conditions in a beautiful new lab. If you are interested, please contact Konni Black. kblack@digestivehlth.com Thank you for your interest. From Ashley.Troutman <@t> Vanderbilt.Edu Mon May 2 11:46:29 2011 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Mon May 2 11:46:35 2011 Subject: [Histonet] Tumors tumors everywhere Message-ID: <7B310892042DA74CB3590053F424CFE613D63CD2DF@ITS-HCWNEM06.ds.Vanderbilt.edu> Hi Sarah, Biocare's MACH3 is designed to work with mouse or rabbit antibodies, so it is likely picking up some mouse antigens: http://biocare.net/products/detection/mach-3/ MACH 3(tm) MACH 3 is a two-step, biotin-free detection system which provides excellent specificity, sensitivity and nuclear staining for mouse or rabbit primary antibodies. I would choose a reagent that recognizes ONLY your rabbit antibody. Check out Biocare's Starr Trek components (like the Trekkie Rabbit link. I know the name is goofy... :)). They should be able to help you. Good luck. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 7 Date: Thu, 28 Apr 2011 15:16:16 -0500 From: Subject: [Histonet] Tumors tumors everywhere To: Message-ID: Content-Type: text/plain; charset="us-ascii" So I am staining tumors that were implanted as cells subdermally into mice. The cells are human. I am trying to do Caspase staining on these tumors. The primary is an anti-human rabbit polyclonal, and I am using a polymer (Biocare Mach3) in lieu of the secondary antibody. The background is through the roof!! Could the reason be that the tumor was grown in a mouse and is having cross reactivity somehow? What species antibody should I be using instead? All my mouse monoclonal antibodies work perfect on the tissue, it's this stupid rabbit polyclonal!! I am blocking endogenous enzymes (peroxidase etc., DAKO), avidin and biotin (just to see if that would help...it didn't), and protein block (it's literally an hour worth of blocking!!), developing with DAB (Dako) and hematoxylin counterstain. I am so confused as how to get this to work! Also, it isn't just this particular antibody it is any rabbit polyclonal I have tried. Could it be the polymer? It is the one that Biocare suggested? HELP!! Thanks in advance =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From mmooreht <@t> yahoo.com Mon May 2 11:48:43 2011 From: mmooreht <@t> yahoo.com (Michelle Moore) Date: Mon May 2 11:48:47 2011 Subject: [Histonet] Request from the Caribbean! Message-ID: <162179.89624.qm@web57411.mail.re1.yahoo.com> Good Afternoon Histonetters! I have started to build a control block bank and wow do I need some help! I know that it sounds ridiculous for anyone living on an island in the Caribbean to have a complaint, but wow is it hard to find good control blocks from such a limited source! I use to belong to the fantastic Michigan Society that has an awesome sharing program with tissue blocks. I have worked on the lovely island of St. Thomas in the US?Virgin Islands for going on five years and no longer belong to any Stateside Societies.? I can offer a thank you and an invitation to bring the blocks down personally for a visit to the Caribbean! I am in need of fungus,?AFB and H. Pylori. Any help would be fantastic and very appreciated! ? Michelle Moore Histopathology/Cytology Supervisor Schneider Regional Medical Center 9048 Sugar Estate, ST. Thomas, US VI 00802 340-776-8311x1047 mlmoore@srmedicalcenter.org From jm.lapointe <@t> accellab.com Mon May 2 12:24:25 2011 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Mon May 2 12:24:29 2011 Subject: [Histonet] Tumors tumors everywhere In-Reply-To: <201105021704.p42H4cUk017843@gateway8.lastspam.com> References: <201105021704.p42H4cUk017843@gateway8.lastspam.com> Message-ID: Hi Sarah, in a previous job my lab did extensive IHC staining on mouse xenografts of human tumors, like your tissue. We never used mouse monoclonal primaries on this, because of the background issue. Even with a kit designed to supposedly block the non-specific mouse cross-reactivity, the results were not good the few times we tried it. When using a rabbit poly (or rat mono) as primary, and the appropriate detection system, non-specific background was never a problem. So like Ashley says, your problem is probably with your MACH3 detection system, which seems to pick up mouse antigens. Use a rabbit-specific one, and your problem should go away. Good luck, __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Qu?bec, Canada J7H 1N8 tel:? 450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com ? ? ------------------------------ Message: 10 Date: Mon, 2 May 2011 11:46:29 -0500 From: "Troutman, Kenneth A" Subject: Re: [Histonet] Tumors tumors everywhere To: "Histonet@lists.utsouthwestern.edu" Message-ID: <7B310892042DA74CB3590053F424CFE613D63CD2DF@ITS-HCWNEM06.ds.Vanderbilt.edu> Content-Type: text/plain; charset="us-ascii" Hi Sarah, Biocare's MACH3 is designed to work with mouse or rabbit antibodies, so it is likely picking up some mouse antigens: http://biocare.net/products/detection/mach-3/ MACH 3(tm) MACH 3 is a two-step, biotin-free detection system which provides excellent specificity, sensitivity and nuclear staining for mouse or rabbit primary antibodies. I would choose a reagent that recognizes ONLY your rabbit antibody. Check out Biocare's Starr Trek components (like the Trekkie Rabbit link. I know the name is goofy... :)). They should be able to help you. Good luck. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 7 Date: Thu, 28 Apr 2011 15:16:16 -0500 From: Subject: [Histonet] Tumors tumors everywhere To: Message-ID: Content-Type: text/plain; charset="us-ascii" So I am staining tumors that were implanted as cells subdermally into mice. The cells are human. I am trying to do Caspase staining on these tumors. The primary is an anti-human rabbit polyclonal, and I am using a polymer (Biocare Mach3) in lieu of the secondary antibody. The background is through the roof!! Could the reason be that the tumor was grown in a mouse and is having cross reactivity somehow? What species antibody should I be using instead? All my mouse monoclonal antibodies work perfect on the tissue, it's this stupid rabbit polyclonal!! I am blocking endogenous enzymes (peroxidase etc., DAKO), avidin and biotin (just to see if that would help...it didn't), and protein block (it's literally an hour worth of blocking!!), developing with DAB (Dako) and hematoxylin counterstain. I am so confused as how to get this to work! Also, it isn't just this particular antibody it is any rabbit polyclonal I have tried. Could it be the polymer? It is the one that Biocare suggested? HELP!! Thanks in advance =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ***************** From pvlies <@t> yahoo.com Mon May 2 14:11:16 2011 From: pvlies <@t> yahoo.com (Pam V) Date: Mon May 2 14:11:19 2011 Subject: [Histonet] (no subject) Message-ID: <316274.78585.qm@web81102.mail.mud.yahoo.com> http://smkn1rangkasbitung.net/modules/mod_osdonate/radio.html From CThornton <@t> dahlchase.com Mon May 2 14:17:47 2011 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Mon May 2 14:17:52 2011 Subject: [Histonet] Region I Convention/Symposium Message-ID: Please join us at the Region I Convention/Symposium, May 20-21, 2011. It will be at the Hilton Garden Inn in Bangor, Maine. A little further to go but well worth it! Please visit the Maine Society for Histotechnology website at www.mainesocietyofhistotechnology.org for more information and to view and download a registration brochure. Join us, explore...Maine in May! Clare Thornton President, MeSH Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From histotalk <@t> yahoo.com Mon May 2 14:26:19 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Mon May 2 14:26:24 2011 Subject: [Histonet] Region I Convention/Symposium In-Reply-To: References: Message-ID: <651919.7246.qm@web120604.mail.ne1.yahoo.com> Hi Clare - I have been promoting your convention on the show. I'll remind everyone again AND also read your email on this Sunday's show. Yours, Dave ________________________________ From: Clare Thornton To: "Histonet@lists.utsouthwestern.edu" Sent: Mon, May 2, 2011 3:17:47 PM Subject: [Histonet] Region I Convention/Symposium Please join us at the Region I Convention/Symposium, May 20-21, 2011.? It will be at the Hilton Garden Inn in Bangor, Maine.? A little further to go but well worth it!? Please visit the Maine Society for Histotechnology website at www.mainesocietyofhistotechnology.org for more information and to view and download a registration brochure. Join us, explore...Maine in May! Clare Thornton President, MeSH Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Mon May 2 15:02:14 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Mon May 2 15:02:29 2011 Subject: [Histonet] Colorado HTL Study Partner Message-ID: <4DBEB966020000A80005BE0F@ns.luhcares.org> Hey all, I am preparing to take the HTL and was hoping to find a study partner in Colorado, Denver/Boulder/Longmont area. Give me a call or e-mail, Thanks Matt Lunetta, BS HT(ASCP) 303-651-5073 From Montina.VanMeter <@t> pbrc.edu Mon May 2 16:28:50 2011 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Mon May 2 16:28:55 2011 Subject: [Histonet] 2011 Louisiana Society for Histotechnology Annual Symposium/Convention Message-ID: Dear Histonet Colleagues, The Louisiana Society for Histotechnology would like to invite you to our annual Symposium/Conference on June 3 & 4, 2011, in Baton Rouge, LA. Meeting location: The Embassy Suites Hotel 4914 Constitution Ave. Baton Rouge, LA 70808 We have a block of rooms reserved for attendees at the special rate of $99.00. After the cut-off date of May12, 2011, the rooms may be reserved at that rate per availability. The hotel will honor this rate two days prior and two days after our meeting (please contact the Embassy Suites Hotel for further information: 1-225-924-6566 or 1-800-Embassy). A complimentary hotel shuttle service is provided for those flying into Baton Rouge. Great dining, beautiful southern plantations, and gambling boats on the Mississippi River are just a few of the attractions in the Baton Rouge area. Remember to ask for the Louisiana Society for Histotechnology group when placing your reservation. The LSH will accept credit cards through our new PayPal account. If you would like to pay via credit card, please send me your application form and the LSH will email an invoice to you that allows you to input your card information. The LSH will also accept check or cash payments. Walk-ins are always welcome! If you have any questions please contact: Tina Van Meter at 225-603-0953 or vanmetmj@pbrc.edu 2011 LSH Workshops: 1. MOHS Surgery and the Art of Proper Orientation in Embedding 2. In Situ and Assay Development 3. Lab Math, Team Building and Leadership within the Lab. 4. Basic IHC 5. Competency Assessment 6. "The Right Stain" 7. Multi-Systems Made Easy - (IHC) 8. Prostate Cancer and Prostate Cancer IHC Markers - Traditional & New Thank you, The LSH Education Committee From Tony_Reilly <@t> health.qld.gov.au Mon May 2 19:39:09 2011 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Mon May 2 19:39:58 2011 Subject: [Histonet] tissue artifact In-Reply-To: <924580.42756.qm@web37104.mail.mud.yahoo.com> References: <924580.42756.qm@web37104.mail.mud.yahoo.com> Message-ID: <4DBFDB4C.411C.0039.1@health.qld.gov.au> Hi Cindy This artifact can also be as a result of the local anesthetic. In this case there is nothing you can do to prevent it. I am not sure why it does not appear in all samples. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> cindy dewar 4/27/2011 5:54 am >>> I work in a dermpath lab. One day last week we had a few slides that had "vacuoles" or holes next to the nucleus. We had over 600 hundred cases, and it only happened to a few of them. There seems to be nothing in common with them. The biopsies came from different Dr's offices, different companies manufactured the formalin bottles, etc. It only happened one day and since then there have been no issues. Since it does not involve all of the specimens, it doesn't seem to be processing or staining. Could it be from sectioning due to freeze spray? too many sponges?. If anyone has any ideas as to what could cause this, I would greatly appreciate it. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From jucam98 <@t> sbcglobal.net Tue May 3 00:48:48 2011 From: jucam98 <@t> sbcglobal.net (Juan Camarena) Date: Tue May 3 00:48:53 2011 Subject: [Histonet] my IEC CTD cryostat broke I need back up Message-ID: <207618.45315.qm@web80601.mail.mud.yahoo.com> Hi there my IEC CTD cryostast is not working and it will take time to be fixed. Actually,? I am looking to get onother as a back up, somebody knows where can I get one. From jucam98 <@t> sbcglobal.net Tue May 3 01:10:34 2011 From: jucam98 <@t> sbcglobal.net (Juan Camarena) Date: Tue May 3 01:10:36 2011 Subject: [Histonet] I need a IEC CTD cryostat for back up Message-ID: <289053.70337.qm@web80607.mail.mud.yahoo.com> My IEC CTD cryostat stop working and it will take time to be fixed, somebody knows who has an extra IEC CTD cryostat that would be interested to sale it. Any condition it is(I' ll fixed), I want it as a back up. Thanks, Juan Camarena Mohs Tecnician 650 766-2239 jucam98@sbcglobal.net ? From mab70 <@t> medschl.cam.ac.uk Tue May 3 02:52:59 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Tue May 3 02:53:55 2011 Subject: [Histonet] No patient ID: Ink dissolved from Cassettes duringprocessing. In-Reply-To: <45567.85076.qm@web180103.mail.gq1.yahoo.com> References: <45567.85076.qm@web180103.mail.gq1.yahoo.com> Message-ID: Pencil does not dissolve in processing reagents and is safe. I have also found the marker pens from Surgipath to be the best of those I have tried, but with the proviso that the labels should be left to dry for a couple of hours before being immersed in ethanol. I routinely use one of these pens for labelling my slides. The old fashioned way was to put a labelled slip of card or paper into the cassette with the tissue. I don't know how this would fit in with US regulations... Margaret -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kiranjit Grewal Sent: 29 April 2011 22:44 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] No patient ID: Ink dissolved from Cassettes duringprocessing. Hi All, ? What is the standard practice out in histology world?if?hand written cassette id washed?away during processing? ? Please share if you had any experience and how did you resolve this and what is your current practice. ? ? Thank you so much! ? -Kiranjit ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dreynold <@t> mdanderson.org Tue May 3 09:40:46 2011 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Tue May 3 09:41:00 2011 Subject: [Histonet] RE: Histonet Digest, Vol 90, Issue 2 In-Reply-To: References: Message-ID: <785BBF0C5F49CE41BA74460A43A08F022E00517BDA@DCPWVMBXC0VS3.mdanderson.edu> I is my understanding that the Mach 3 is for use on human clinical samples and will not work in mouse tissue. Maybe BioCare technical person didn't realize you were using human cell in the mouse. BioCare has a great polymer for rabbit antibodies on rodent tissue in their ProMark series. "Rabbit on Rodent HRP Polymer" cat RMR622, also comes in AP format RMR625. This is a great product especially when signal is low. Great amplification and no background. I am not aware of the Starr Trek system, going to have to check it out. ------------------------------ Message: 10 Date: Mon, 2 May 2011 11:46:29 -0500 From: "Troutman, Kenneth A" Subject: Re: [Histonet] Tumors tumors everywhere To: "Histonet@lists.utsouthwestern.edu" Message-ID: <7B310892042DA74CB3590053F424CFE613D63CD2DF@ITS-HCWNEM06.ds.Vanderbilt.edu> Content-Type: text/plain; charset="us-ascii" Hi Sarah, Biocare's MACH3 is designed to work with mouse or rabbit antibodies, so it is likely picking up some mouse antigens: http://biocare.net/products/detection/mach-3/ MACH 3(tm) MACH 3 is a two-step, biotin-free detection system which provides excellent specificity, sensitivity and nuclear staining for mouse or rabbit primary antibodies. I would choose a reagent that recognizes ONLY your rabbit antibody. Check out Biocare's Starr Trek components (like the Trekkie Rabbit link. I know the name is goofy... :)). They should be able to help you. Good luck. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 7 Date: Thu, 28 Apr 2011 15:16:16 -0500 From: Subject: [Histonet] Tumors tumors everywhere To: Message-ID: Content-Type: text/plain; charset="us-ascii" So I am staining tumors that were implanted as cells subdermally into mice. The cells are human. I am trying to do Caspase staining on these tumors. The primary is an anti-human rabbit polyclonal, and I am using a polymer (Biocare Mach3) in lieu of the secondary antibody. The background is through the roof!! Could the reason be that the tumor was grown in a mouse and is having cross reactivity somehow? What species antibody should I be using instead? All my mouse monoclonal antibodies work perfect on the tissue, it's this stupid rabbit polyclonal!! I am blocking endogenous enzymes (peroxidase etc., DAKO), avidin and biotin (just to see if that would help...it didn't), and protein block (it's literally an hour worth of blocking!!), developing with DAB (Dako) and hematoxylin counterstain. I am so confused as how to get this to work! Also, it isn't just this particular antibody it is any rabbit polyclonal I have tried. Could it be the polymer? It is the one that Biocare suggested? HELP!! Thanks in advance =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From relia1 <@t> earthlink.net Tue May 3 11:34:13 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue May 3 11:34:11 2011 Subject: [Histonet] RELIA Hot Histology Job Alert 5/3/2011 Exciting new jobs to tell you about! Message-ID: Hi Histonetters!! I hope everybody had a great Lab Week last week. I am talking lots of goodies to eat and fun stuff to do. I have heard of some really cool activities that took place at some of my client sites. If you want some ideas for next year shoot me an e-mail and I would be happy to share! I also would like to tell you about a couple of new positions I am working on. These positions like all the positions we represent at RELIA are full time permanent positions with great compensation benefits and relocation assistance. Here are my newest positions: Assistant Histology Supervisor - New Mexico Day Shift Histology Tech - Milwaukee, WI Night Shift Grossing Tech or PA - Baltimore, MD **I also have positions in FL, NY, ME, CA, MA, TX, NC, OR and LA. If none of these locations strike your fancy I am getting new positions on an almost daily basis so please feel free to shoot me an e-mail and let me know if there is a location you want me to keep a lookout for and I will be happy to do so. If you have friends that are looking please feel free to introduce them to me. If I place them I will pay you a 500 referral fee. Remember I am the only recruiter that works exclusively in nationwide permanent placement of histology professionals. I offer 25 years of experience helping people advance their careers. You have the histology expertise to excel in your field let me put my recruiting expertise to work for you as you consider your next job change or career move. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From nicole <@t> dlcjax.com Tue May 3 13:48:22 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Tue May 3 13:48:32 2011 Subject: [Histonet] Does a Path lab for any reason need a disposal Message-ID: <3906.208.62.167.196.1304448502.squirrel@webmail.realpages.com> Does anyone know, for any reason why a dermatopathology lab would be or is required to have a garbage disposal? Sound like a stupid question, but wanted to make sure... Thanks fellow Histo's Nicole Tatum HT(ASCP) From farnana <@t> nehealth.com Wed May 4 09:42:22 2011 From: farnana <@t> nehealth.com (Amy Farnan) Date: Wed May 4 09:42:32 2011 Subject: [Histonet] New York State Annual Symposium May 14th Message-ID: <4DC12D8E020000D90004789D@neh_domain_app_srv.nehealth.com> Hi Everyone The NYSHS annual meeting is fast approaching and space is filling up. Registration is open and you can get a copy of the meeting program and regsitration form by visiting the NYSHS website at: http://www.nyhisto.org/index.htm Below is a synopsis of the program: Registration opens at 7:00 AM 8:00 AM 1) Digital Pathology 101 Virgil Hernadez CT (ASCP) Digital Pathology Specialist, Ventana Medical Systems 9:00 AM 2) The Role of Histopathology in Forensic Postmortem Investigations Dr. Kari Reiber Chief Medical Examiner, Dutchess County, NY. 10:00 AM 3) Personalized Approach for Non-Small Cell Lung Cancer Joseph Dudek, M.D., US Oncology Incorporated ? New York Oncology Hematology. 11:00-12:00 AM Free time to visit the Vendor Hall 12:00-1:30 PM Awards Luncheon and General Membership Meeting 1:30-2:00 PM Free time to visit the Vendor Hall 2:00 PM 4) The Right Stain, Troubleshooting Histology Stains Valantou Grover, HT, HTL(ASCP), PA, MBA Biosciences Product Line Manager, Polysciences, Inc. 3:00 PM 5) Hard, Harder and Hardest: Choosing the right process foryour bone project Susan Ryan, HTL(ASCP), Genzyme, Inc. 4:00 PM 6) Formalin Recycling: "Is your lab safe?" Amy Farnan, HT (ASCP) Supervisor, Histology: Albany Memorial Hospital/Samaritan Hospital, North East Health __._,_.___ Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From kiran_g <@t> sbcglobal.net Wed May 4 09:46:13 2011 From: kiran_g <@t> sbcglobal.net (kiran_g@sbcglobal.net) Date: Wed May 4 09:46:22 2011 Subject: [Histonet] No patient ID: Ink dissolved from Cassettes duringprocessing. In-Reply-To: References: <45567.85076.qm@web180103.mail.gq1.yahoo.com> Message-ID: <1184344281-1304520374-cardhu_decombobulator_blackberry.rim.net-1291307191-@bda2889.bisx.prod.on.blackberry> Thank you all for excellent input and sharing your experience. Hope this doesn't happen again! - Kiranjit Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Margaret Blount" Date: Tue, 3 May 2011 08:52:59 To: Kiranjit Grewal; Subject: RE: [Histonet] No patient ID: Ink dissolved from Cassettes duringprocessing. Pencil does not dissolve in processing reagents and is safe. I have also found the marker pens from Surgipath to be the best of those I have tried, but with the proviso that the labels should be left to dry for a couple of hours before being immersed in ethanol. I routinely use one of these pens for labelling my slides. The old fashioned way was to put a labelled slip of card or paper into the cassette with the tissue. I don't know how this would fit in with US regulations... Margaret -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kiranjit Grewal Sent: 29 April 2011 22:44 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] No patient ID: Ink dissolved from Cassettes duringprocessing. Hi All, ? What is the standard practice out in histology world?if?hand written cassette id washed?away during processing? ? Please share if you had any experience and how did you resolve this and what is your current practice. ? ? Thank you so much! ? -Kiranjit ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Wed May 4 10:51:11 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Wed May 4 10:51:14 2011 Subject: [Histonet] 2011 Louisiana Society for Histotechnology Annual Symposium/Convention In-Reply-To: References: Message-ID: <502090.29745.qm@web120618.mail.ne1.yahoo.com> Hi Montina - HistoTALK will begin plugging the LSH Symposium/Convention beginning this week, May 8th. Every little bit helps. Yours, Dave www.HistoTALK.com ? ________________________________ From: Montina Van Meter To: Histonet@lists.utsouthwestern.edu Sent: Mon, May 2, 2011 5:28:50 PM Subject: [Histonet] 2011 Louisiana Society for Histotechnology Annual Symposium/Convention Dear Histonet Colleagues, The Louisiana Society for Histotechnology would like to invite you to our annual Symposium/Conference on June 3 & 4, 2011, in Baton Rouge, LA. Meeting location:? The Embassy Suites Hotel ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 4914 Constitution Ave. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Baton Rouge, LA? 70808? We have a block of rooms reserved for attendees at the special rate of $99.00. After the cut-off date of May12, 2011, the rooms may be reserved at that rate per availability.? The hotel will honor this rate two days prior and two days after our meeting (please contact the Embassy Suites Hotel for further information: 1-225-924-6566 or 1-800-Embassy).? A complimentary hotel shuttle service is provided for those flying into Baton Rouge. Great dining, beautiful southern plantations, and gambling boats on the Mississippi River are just a few of the attractions in the Baton Rouge area. Remember to ask for the Louisiana Society for Histotechnology group when placing your reservation. The LSH will accept credit cards through our new PayPal account. If you would like to pay via credit card, please send me your application form and the LSH will email an invoice to you that allows you to input your card information. The LSH will also accept check or cash payments. Walk-ins are always welcome!? If you have any questions please contact:? Tina Van Meter at 225-603-0953 or vanmetmj@pbrc.edu 2011 LSH Workshops: 1.? ? MOHS Surgery and the Art of Proper Orientation in Embedding 2.? ? In Situ and Assay Development 3.? ? Lab Math, Team Building and Leadership within the Lab. 4.? ? Basic IHC 5.? ? Competency Assessment 6.? ? "The Right Stain" 7.? ? Multi-Systems Made Easy - (IHC) 8.? ? Prostate Cancer and Prostate Cancer IHC Markers - Traditional & New Thank you, The LSH Education Committee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Wed May 4 10:56:40 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Wed May 4 10:56:45 2011 Subject: [Histonet] New York State Annual Symposium May 14th In-Reply-To: <4DC12D8E020000D90004789D@neh_domain_app_srv.nehealth.com> References: <4DC12D8E020000D90004789D@neh_domain_app_srv.nehealth.com> Message-ID: <468782.81582.qm@web120609.mail.ne1.yahoo.com> It sounds like a great program line-up. I've been promoting it the last two shows and will be sure to plug it again this week, May 8th. I'll read your email on this week's show. :- ) Yours, Dave www.HistoTALK.com ________________________________ From: Amy Farnan To: histonet@lists.utsouthwestern.edu Sent: Wed, May 4, 2011 10:42:22 AM Subject: [Histonet] New York State Annual Symposium May 14th Hi Everyone The NYSHS annual meeting is fast approaching and space is filling up. Registration is open and you can get a copy of the meeting program and regsitration form by visiting the NYSHS website at: http://www.nyhisto.org/index.htm Below is a synopsis of the program: Registration opens at 7:00 AM 8:00 AM 1) Digital Pathology 101 Virgil Hernadez CT (ASCP) Digital Pathology Specialist, Ventana Medical Systems 9:00 AM 2) The Role of Histopathology in Forensic Postmortem Investigations Dr. Kari Reiber Chief Medical Examiner, Dutchess County, NY. 10:00 AM 3) Personalized Approach for Non-Small Cell Lung Cancer Joseph Dudek, M.D., US Oncology Incorporated ? New York Oncology Hematology. 11:00-12:00 AM Free time to visit the Vendor Hall 12:00-1:30 PM Awards Luncheon and General Membership Meeting 1:30-2:00 PM Free time to visit the Vendor Hall 2:00 PM 4) The Right Stain, Troubleshooting Histology Stains Valantou Grover, HT, HTL(ASCP), PA, MBA Biosciences Product Line Manager, Polysciences, Inc. 3:00 PM 5) Hard, Harder and Hardest: Choosing the right process foryour bone project Susan Ryan, HTL(ASCP), Genzyme, Inc. 4:00 PM 6) Formalin Recycling: "Is your lab safe?" Amy Farnan, HT (ASCP) Supervisor, Histology: Albany Memorial Hospital/Samaritan Hospital, North East Health ? ? ? ? ? ? ? ? __._,_.___ Disclaimer: The information in this message is confidential.? If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FUNKM <@t> mercyhealth.com Wed May 4 11:54:29 2011 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Wed May 4 11:54:36 2011 Subject: [Histonet] Her/2 Message-ID: <4DC13E75.E948.00AC.1@mercyhealth.com> CAP validation Her/2 slides not staining as clear as before. Is anyone else having any issues ? We use the Ventana system and our daily IHC slides are staining and controls are working well. Is there a coating on these slides from CAP ? Thanks for any help ? Marcia Funk HT,QIHC Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 From marktarango <@t> gmail.com Wed May 4 12:03:16 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed May 4 12:03:19 2011 Subject: [Histonet] Her/2 In-Reply-To: <4DC13E75.E948.00AC.1@mercyhealth.com> References: <4DC13E75.E948.00AC.1@mercyhealth.com> Message-ID: Hi Marcia, Our slides for the Her2 CAP survey stained just fine. We're using a Ventana XT to stain for Her2 by IHC. Mark On Wed, May 4, 2011 at 9:54 AM, Marcia Funk wrote: > CAP validation Her/2 slides not staining as clear as before. Is anyone > else having any issues ? We use the Ventana system and our daily IHC slides > are staining > and controls are working well. Is there a coating on these slides from CAP > ? > Thanks for any help ? > Marcia Funk HT,QIHC > > > > Marcia Funk > Histology Laboratory > Mercy Medical Center North Iowa > Mason City, IA, 50401 > 641-428-7907 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Wed May 4 12:27:42 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed May 4 12:27:50 2011 Subject: [Histonet] sox 11 mantlecell lymphoma Message-ID: <1CAA151EC6814520A5B9505FD6373DAC@dielangs.at> Hi all! I?m looking for a SOX11 IHC-protocol and for feedback on this special antibody. We want to stain human FFPET and purchased the polyclonal SOX11 antibody from Abcam. My protocol on Benchmark Ultra: CC1 mild, 32 min, 37?C; 1:1000 We found more positivity than expected. Has anyone similar experiences? Thanks in advance Gudrun Lang From Rcartun <@t> harthosp.org Wed May 4 12:34:59 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed May 4 12:35:11 2011 Subject: [Histonet] Her/2 In-Reply-To: <4DC13E75.E948.00AC.1@mercyhealth.com> References: <4DC13E75.E948.00AC.1@mercyhealth.com> Message-ID: <4DC15602.7400.0077.1@harthosp.org> Yes, our slides look weak. I did slides for another hospital and they look weak as well. I have already contacted the CAP to see if they have received any other complaints. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Marcia Funk" 5/4/2011 12:54 PM >>> CAP validation Her/2 slides not staining as clear as before. Is anyone else having any issues ? We use the Ventana system and our daily IHC slides are staining and controls are working well. Is there a coating on these slides from CAP ? Thanks for any help ? Marcia Funk HT,QIHC Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From igor.deyneko <@t> gmail.com Wed May 4 16:05:31 2011 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Wed May 4 16:05:34 2011 Subject: [Histonet] Slide and Cassette Labeling systems Message-ID: Hello Everyone! I would like to get your opinion on a good slide and cassette labeling systems. We are trying to incorporate them in our routine histology lab. We are currently using Aperio system as well and maybe wanted to be able to link the slide labeling via bar code to Aperio database as well. Please comment on specifications and also the vendors. Thank you very much. Igor Deyneko. Infinity Pharmaceuticals Cambridge, MA. From ervelazquez <@t> gmail.com Wed May 4 16:36:55 2011 From: ervelazquez <@t> gmail.com (Eric Velazquez) Date: Wed May 4 16:36:59 2011 Subject: [Histonet] ASCP Route 2 HT Certification Message-ID: Hi, I was hoping someone could help me with the following questions: 1) What happens to the money if I were to submit my transcript and my fees for the exam and end up not qualifying? Do I lose my money or is it credited for when I do qualify? 2) How do I determine what classes are considered BIO and Chem? Does the prefix for the course ID have to contain BIO or Chem? 3) Can the courses taken be similar? Example: School A: I took Chem10: Intro to Chemistry & School B: Chm:3 Chemistry basics. If these classes are interchangeable between schools do they still count or does it have to be a more difficult chemistry course for it to qualify? 4)Is there anyway to check if you do qualify before paying exam fees? 5)Do you have to pass the class with a C or higher? I took a BIO class years ago and received a D, but I was given the credited hours for the course. Route 2 for HT certification for ASCP At least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, or an associate degree from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years. Thanks, Eric From ervelazquez <@t> gmail.com Wed May 4 16:37:46 2011 From: ervelazquez <@t> gmail.com (Eric Velazquez) Date: Wed May 4 16:37:49 2011 Subject: [Histonet] Cryostat Maintenance and Repair - CA Message-ID: Hi, Can somebody please recommend to me a company that does cryostat maintenance and repair? I am looking for a company that is familiar with both Micron and Leica models in the Irvine, CA area. Thanks, Eric From flnails <@t> texaschildrens.org Wed May 4 16:42:00 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed May 4 16:42:10 2011 Subject: [Histonet] ASCP Route 2 HT Certification In-Reply-To: References: Message-ID: Your best answer will come from ASCP so contact them directly. The number should be on the application -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez Sent: Wednesday, May 04, 2011 4:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASCP Route 2 HT Certification Hi, I was hoping someone could help me with the following questions: 1) What happens to the money if I were to submit my transcript and my fees for the exam and end up not qualifying? Do I lose my money or is it credited for when I do qualify? 2) How do I determine what classes are considered BIO and Chem? Does the prefix for the course ID have to contain BIO or Chem? 3) Can the courses taken be similar? Example: School A: I took Chem10: Intro to Chemistry & School B: Chm:3 Chemistry basics. If these classes are interchangeable between schools do they still count or does it have to be a more difficult chemistry course for it to qualify? 4)Is there anyway to check if you do qualify before paying exam fees? 5)Do you have to pass the class with a C or higher? I took a BIO class years ago and received a D, but I was given the credited hours for the course. Route 2 for HT certification for ASCP At least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, or an associate degree from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years. Thanks, Eric _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From jaylundgren <@t> gmail.com Wed May 4 16:52:00 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed May 4 16:52:04 2011 Subject: [Histonet] ASCP Route 2 HT Certification In-Reply-To: References: Message-ID: Uhhh, do you really think it's wise to post the fact that you got a D in Bio on a public forum, under your real name? Good luck on the exam, Jay A. Lundgren, M.S., HTL (ASCP) From ervelazquez <@t> gmail.com Wed May 4 16:51:53 2011 From: ervelazquez <@t> gmail.com (Eric Velazquez) Date: Wed May 4 16:52:26 2011 Subject: [Histonet] ASCP Route 2 HT Certification In-Reply-To: References: Message-ID: Thank you for responding. I agree with you that the ASCP would be the best source of information, but unfortunately I spoke with someone who was in charge of reviewing the applications. She was unprofessional and offered little assistance. I will attempt to contact them again, but finding the time to be placed on hold for 30mins while I am at work is difficult. I was hoping someone would know from experience or perhaps someone who is involved with the process would be able to offer some guidance. -Eric On Wed, May 4, 2011 at 2:42 PM, Nails, Felton wrote: > Your best answer will come from ASCP so contact them directly. The number > should be on the application > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez > Sent: Wednesday, May 04, 2011 4:37 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] ASCP Route 2 HT Certification > > Hi, > > I was hoping someone could help me with the following questions: > > 1) What happens to the money if I were to submit my transcript and my fees > for the exam and end up not qualifying? Do I lose my money or is it credited > for when I do qualify? > 2) How do I determine what classes are considered BIO and Chem? Does the > prefix for the course ID have to contain BIO or Chem? > 3) Can the courses taken be similar? Example: School A: I took Chem10: > Intro to Chemistry & School B: Chm:3 Chemistry basics. If these classes are > interchangeable between schools do they still count or does it have to be a > more difficult chemistry course for it to qualify? > 4)Is there anyway to check if you do qualify before paying exam fees? > 5)Do you have to pass the class with a C or higher? I took a BIO class > years ago and received a D, but I was given the credited hours for the > course. > > > Route 2 for HT certification for ASCP > At least 60 semester hours (90 quarter hours) of academic credit from a > regionally accredited college/university, with a combination of 12 semester > hours (18 quarter hours) of biology and chemistry, or an associate degree > from a regionally accredited college/university, with a combination of 12 > semester hours (18 quarter hours) of biology and chemistry, AND one year > full time acceptable experience in a histopathology (clinical, veterinary, > industry or research) laboratory in the U.S., Canada or an accredited > laboratory* within the last ten years. > > Thanks, > Eric > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ______________________________________________________________________ > From laurie.colbert <@t> huntingtonhospital.com Wed May 4 16:58:52 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed May 4 16:58:55 2011 Subject: [Histonet] Cryostat Maintenance and Repair - CA In-Reply-To: References: Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AC01@EXCHANGE3.huntingtonhospital.com> Hi Eric, Mikron Instruments services most of our instruments, including Micron brand. I don't have any Leica equipment now, but they have serviced my Leica microtome in the past. They are very good! The service manager's name is Steve Pike. Mikron is in Vista, CA and their phone number is (800) 377-5395. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez Sent: Wednesday, May 04, 2011 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Maintenance and Repair - CA Hi, Can somebody please recommend to me a company that does cryostat maintenance and repair? I am looking for a company that is familiar with both Micron and Leica models in the Irvine, CA area. Thanks, Eric _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ervelazquez <@t> gmail.com Wed May 4 17:06:00 2011 From: ervelazquez <@t> gmail.com (Eric Velazquez) Date: Wed May 4 17:06:03 2011 Subject: [Histonet] ASCP Route 2 HT Certification In-Reply-To: References: Message-ID: First class, first quarter in college. Should have taken it more seriously, but everyone makes mistakes and learns from them. Thanks for your input. -Eric On Wed, May 4, 2011 at 2:52 PM, Jay Lundgren wrote: > Uhhh, do you really think it's wise to post the fact that you got a D in > Bio on a public forum, under your real name? > > Good luck on the exam, > > Jay A. > Lundgren, M.S., HTL (ASCP) > > > > > > From hrumbut <@t> yahoo.com Wed May 4 21:33:17 2011 From: hrumbut <@t> yahoo.com (Heather R) Date: Wed May 4 21:33:21 2011 Subject: [Histonet] Poor contrast in H&E stain Message-ID: <996545.43931.qm@web130223.mail.mud.yahoo.com> I am in the process of setting up stain run for our new stainer and am having a problem getting "crisp" contrast in our H&E.? I have adjusted times with little improvement. The stain looks "muddy" I guess. The pathologist says it's the contrast.? We are doing skin biopsies. Using Xylene, Gills, richard allen clarifier , doesnt seem like this should be too hard, but it's getting frustrating. Thanks HR From njblademaster <@t> gmail.com Wed May 4 22:10:28 2011 From: njblademaster <@t> gmail.com (Nathan Jentsch) Date: Wed May 4 22:10:51 2011 Subject: [Histonet] Re: Does a Path lab for any reason need a disposal Message-ID: Nicole, I work in a dermpath lab in New York State where regulations are so unbelievable that I often wake up in night sweats, and I can think of no reason to need a garbage disposal. We've certainly never been told we need one or cited for not having one, and believe me, they would if they thought it was necessary. Nathan Jentsch BS HT(ASCP) From clb1158 <@t> yahoo.com Thu May 5 04:32:20 2011 From: clb1158 <@t> yahoo.com (C B) Date: Thu May 5 04:32:22 2011 Subject: [Histonet] bone ground sections Message-ID: <450867.4158.qm@web114016.mail.gq1.yahoo.com> What hematoxylin is best for doing H&E on ground sections of undecalcified bone-?one that?won't leave a precipiate on the bone? Cindy Baranowski SJTRI Atlanta, GA From bakevictoria <@t> gmail.com Thu May 5 07:25:44 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu May 5 07:25:47 2011 Subject: [Histonet] Use of EDTA to soften tissue (non - calcified) Message-ID: Good morning, I'm a little stumped here - no it's not a first I know. I've come across a lab that uses EDTA powder in their ice baths to soften GI biopsies - I've never heard of this before. I've used it for bone or calcifications when nothing else was available for a surface decal and didn't like how it would sometimes affect staining. Thanks in advance for any information. Vikki From estellamireles <@t> gmail.com Thu May 5 07:49:57 2011 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Thu May 5 07:50:00 2011 Subject: [Histonet] Americlear Staining Problems Message-ID: Good Morning Histonetters, Our H&E stained slides are bleeding out the eosin. No, I did not shoot the sheriff. Called the manufacturer, and was advised to: Give more time in the Alcohols, Use only 100% Alcohols after eosin. Change the last two Alcohols and Americlear. Done. Done. Done. Stain bleeding really improved, but still shows up on hit and miss cases. Has anyone had this problem and How did you fix it Stella From trathborne <@t> somerset-healthcare.com Thu May 5 08:12:20 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu May 5 08:12:53 2011 Subject: [Histonet] ASCP Route 2 HT Certification In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707E9FC@SMCMAIL01.somerset-healthcare.com> You could email them. It would not be so time consuming, and then you would have proof of an answer should anything be questioned at a later date. Good luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez Sent: Wednesday, May 04, 2011 5:52 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP Route 2 HT Certification Thank you for responding. I agree with you that the ASCP would be the best source of information, but unfortunately I spoke with someone who was in charge of reviewing the applications. She was unprofessional and offered little assistance. I will attempt to contact them again, but finding the time to be placed on hold for 30mins while I am at work is difficult. I was hoping someone would know from experience or perhaps someone who is involved with the process would be able to offer some guidance. -Eric On Wed, May 4, 2011 at 2:42 PM, Nails, Felton wrote: > Your best answer will come from ASCP so contact them directly. The > number should be on the application > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez > Sent: Wednesday, May 04, 2011 4:37 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] ASCP Route 2 HT Certification > > Hi, > > I was hoping someone could help me with the following questions: > > 1) What happens to the money if I were to submit my transcript and my > fees for the exam and end up not qualifying? Do I lose my money or is > it credited for when I do qualify? > 2) How do I determine what classes are considered BIO and Chem? Does > the prefix for the course ID have to contain BIO or Chem? > 3) Can the courses taken be similar? Example: School A: I took Chem10: > Intro to Chemistry & School B: Chm:3 Chemistry basics. If these > classes are interchangeable between schools do they still count or > does it have to be a more difficult chemistry course for it to qualify? > 4)Is there anyway to check if you do qualify before paying exam fees? > 5)Do you have to pass the class with a C or higher? I took a BIO class > years ago and received a D, but I was given the credited hours for the > course. > > > Route 2 for HT certification for ASCP > At least 60 semester hours (90 quarter hours) of academic credit from > a regionally accredited college/university, with a combination of 12 > semester hours (18 quarter hours) of biology and chemistry, or an > associate degree from a regionally accredited college/university, with > a combination of 12 semester hours (18 quarter hours) of biology and > chemistry, AND one year full time acceptable experience in a > histopathology (clinical, veterinary, industry or research) laboratory > in the U.S., Canada or an accredited > laboratory* within the last ten years. > > Thanks, > Eric > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an authorized > representative of the intended recipient, you are hereby notified > that any review, dissemination, or copying of this e-mail and its > attachments, if any, or the information contained herein is > prohibited. If you have received this e-mail in error, please > immediately notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ______________________________________________________________________ > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From dlaudier <@t> gmail.com Thu May 5 08:27:44 2011 From: dlaudier <@t> gmail.com (Damien Laudier) Date: Thu May 5 08:27:48 2011 Subject: [Histonet] Re: Bone ground sections Message-ID: Hi Cindy, There is really no "best" hematoxylin,as any of the popular commercial formulations can be used successfully on ground sections. The key to reducing/eliminating the precipitation problem is filtering the hematoxylin really well before staining and sonicating your sections after staining. For darker nuclear staining,I like Gill III or Ehrlich's. The Celestine Blue-Hematoxylin technique also works well on ground sections. -Damien L. From rjbuesa <@t> yahoo.com Thu May 5 08:53:49 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 5 08:53:53 2011 Subject: [Histonet] Use of EDTA to soften tissue (non - calcified) In-Reply-To: References: Message-ID: <328155.39528.qm@web65709.mail.ac4.yahoo.com> EDTA is a chelating agent whose action is to remove calcium ions from the tissue. Since GI biopsies do not contain calcium this procedure can be considered part of some "Vudu" practices (or "snake oil" medicament). Ren? J. From: Victoria Baker To: Histo Net list server Sent: Thursday, May 5, 2011 8:25 AM Subject: Re: [Histonet] Use of EDTA to soften tissue (non - calcified) Good morning, I'm a little stumped here - no it's not a first I know. I've come across a lab that uses EDTA powder in their ice baths to soften GI biopsies - I've never heard of this before. I've used it for bone or calcifications when nothing else was available for a surface decal and didn't like how it would sometimes affect staining. Thanks in advance for any information. Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu May 5 10:17:13 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu May 5 10:17:16 2011 Subject: [Histonet] Americlear Staining Problems In-Reply-To: References: Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AC08@EXCHANGE3.huntingtonhospital.com> It's usually due to water on the slides. If your alcohols and Americlear are clean (water-free), check to see that your slides are totally submerged in these two reagents, and there is not water dripping down the slide. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Thursday, May 05, 2011 5:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Americlear Staining Problems Good Morning Histonetters, Our H&E stained slides are bleeding out the eosin. No, I did not shoot the sheriff. Called the manufacturer, and was advised to: Give more time in the Alcohols, Use only 100% Alcohols after eosin. Change the last two Alcohols and Americlear. Done. Done. Done. Stain bleeding really improved, but still shows up on hit and miss cases. Has anyone had this problem and How did you fix it Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu May 5 10:46:11 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu May 5 10:46:18 2011 Subject: AW: [Histonet] sox 11 mantlecell lymphoma erratum In-Reply-To: <1CAA151EC6814520A5B9505FD6373DAC@dielangs.at> References: <1CAA151EC6814520A5B9505FD6373DAC@dielangs.at> Message-ID: <151E9B1027F54F2396436F5783D13609@dielangs.at> Sorry, I told you the wrong vendor of the antibody. The correct one is Acris Antibodies. http://m1.acris-antibodies.com/pdf/AP09577PU-N.pdf Hi all! I’m looking for a SOX11 IHC-protocol and for feedback on this special antibody. We want to stain human FFPET and purchased the polyclonal SOX11 antibody from Acris. My protocol on Benchmark Ultra: CC1 mild, 32 min, 37°C; 1:1000 We found more positivity and nuclear staining than expected (not only in mantle cell lymphoma, but also in follikular lymphoma and diffuse large cell lymphoma). Has anyone similar experiences? Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From azdudley <@t> hotmail.com Thu May 5 11:37:28 2011 From: azdudley <@t> hotmail.com (anita dudley) Date: Thu May 5 11:37:33 2011 Subject: [Histonet] Her/2 In-Reply-To: <4DC13E75.E948.00AC.1@mercyhealth.com> References: <4DC13E75.E948.00AC.1@mercyhealth.com> Message-ID: marcia, was just getting ready to stain our her 2's , will let you know how they look. anita dudley providence hosp mobile, al > Date: Wed, 4 May 2011 12:54:29 -0400 > From: FUNKM@mercyhealth.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Her/2 > > CAP validation Her/2 slides not staining as clear as before. Is anyone else having any issues ? We use the Ventana system and our daily IHC slides are staining > and controls are working well. Is there a coating on these slides from CAP ? > Thanks for any help ? > Marcia Funk HT,QIHC > > > > Marcia Funk > Histology Laboratory > Mercy Medical Center North Iowa > Mason City, IA, 50401 > 641-428-7907 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From azdudley <@t> hotmail.com Thu May 5 11:43:45 2011 From: azdudley <@t> hotmail.com (anita dudley) Date: Thu May 5 11:43:50 2011 Subject: [Histonet] ANP 11630 Message-ID: just wondering if anyone out there would send their procedure for how they handle this. do you have a list with each type the tech can put on and if it is indirect or direct. thanks so much. and also is everyone taking their cryostats down every week and if not have you been cited? thanks anita dudley providence hosp mobile, al From dencrowl <@t> MIT.EDU Thu May 5 12:21:36 2011 From: dencrowl <@t> MIT.EDU (Denise G Crowley) Date: Thu May 5 12:21:43 2011 Subject: [Histonet] NSH symposium registration Message-ID: <9B07EB40-F58E-4181-923A-F8CFB3D2C687@mit.edu> Hi all, Does anyone know if we will be receiving booklets from NSH or if everything is to be done on line? Denise Crowley Histology Facility Manager Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu From akbitting <@t> geisinger.edu Thu May 5 12:30:14 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu May 5 12:30:29 2011 Subject: [Histonet] Hep B Message-ID: <4DC2A666.2B7F.00C9.1@geisinger.edu> I'm looking for hepatitis B positive control tissue. Willing to trade if I have tissue that you might want. Contact me off line. Thanks. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From JMacDonald <@t> mtsac.edu Thu May 5 13:18:08 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu May 5 13:18:16 2011 Subject: [Histonet] NSH symposium registration In-Reply-To: <9B07EB40-F58E-4181-923A-F8CFB3D2C687@mit.edu> Message-ID: they were mailed out. Denise G Crowley Sent by: histonet-bounces@lists.utsouthwestern.edu 05/05/2011 10:24 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] NSH symposium registration Hi all, Does anyone know if we will be receiving booklets from NSH or if everything is to be done on line? Denise Crowley Histology Facility Manager Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu May 5 13:44:53 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu May 5 13:44:56 2011 Subject: [Histonet] NSH symposium registration In-Reply-To: <9B07EB40-F58E-4181-923A-F8CFB3D2C687@mit.edu> References: <9B07EB40-F58E-4181-923A-F8CFB3D2C687@mit.edu> Message-ID: <00df01cc0b54$857f90b0$907eb210$@northwestern.edu> Try info@nsh.org. They'll let you and us know. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Denise G Crowley Sent: Thursday, May 05, 2011 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH symposium registration Hi all, Does anyone know if we will be receiving booklets from NSH or if everything is to be done on line? Denise Crowley Histology Facility Manager Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jayne.Halli <@t> avera.org Thu May 5 14:01:17 2011 From: Jayne.Halli <@t> avera.org (Jayne Halli) Date: Thu May 5 14:04:55 2011 Subject: [Histonet] Re: Her2 CAP survey Message-ID: Cap validation Her2 slides we got one slide stained great, the other one did not, controls and cases all stained good. We use the Dako system. Jayne Halli HT,ASCP,QIHC Avera McKennan Histology 800 East 21st street Sioux Falls, SD 57117 (605)322-7142 my new e-mail address is jayne.halli@avera.org ----------------------------------------- Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. From cindy.deriso <@t> yale.edu Thu May 5 14:11:42 2011 From: cindy.deriso <@t> yale.edu (Cindy DeRiso) Date: Thu May 5 14:11:45 2011 Subject: [Histonet] Safety glasses required for embedding Message-ID: <4DC2F66E.8060102@yale.edu> I am not sure if this has come up before, but does anyone require personnel to wear safety glasses when embedding and if so is there an OSHA policy? Thanks Cindy From rjbuesa <@t> yahoo.com Thu May 5 14:46:20 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 5 14:46:24 2011 Subject: [Histonet] Safety glasses required for embedding In-Reply-To: <4DC2F66E.8060102@yale.edu> References: <4DC2F66E.8060102@yale.edu> Message-ID: <361030.45536.qm@web65712.mail.ac4.yahoo.com> No. Rern? J. From: Cindy DeRiso To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 5, 2011 3:11 PM Subject: Re: [Histonet] Safety glasses required for embedding I am not sure if this has come up before, but does anyone require personnel to wear safety glasses when embedding and if so is there an OSHA policy? Thanks Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu May 5 15:06:03 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu May 5 15:06:12 2011 Subject: [Histonet] Re: Her2 CAP survey In-Reply-To: References: Message-ID: <4DC2CAEB.7400.0077.1@harthosp.org> Everyone who experiencing problems with the CAP HER2 survey slides needs to contact the CAP to document the problem and request replacement slides. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Jayne Halli" 5/5/2011 3:01 PM >>> Cap validation Her2 slides we got one slide stained great, the other one did not, controls and cases all stained good. We use the Dako system. Jayne Halli HT,ASCP,QIHC Avera McKennan Histology 800 East 21st street Sioux Falls, SD 57117 (605)322-7142 my new e-mail address is jayne.halli@avera.org ----------------------------------------- Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Beatrice.Debrosse-Serra <@t> pfizer.com Thu May 5 15:07:30 2011 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Thu May 5 15:07:40 2011 Subject: [Histonet] Safety glasses required for embedding In-Reply-To: <4DC2F66E.8060102@yale.edu> References: <4DC2F66E.8060102@yale.edu> Message-ID: Yes. Beatrice DeBrosse-Serra HT(ASCP)QIHC Investigative Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DeRiso Sent: Thursday, May 05, 2011 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety glasses required for embedding I am not sure if this has come up before, but does anyone require personnel to wear safety glasses when embedding and if so is there an OSHA policy? Thanks Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ervelazquez <@t> gmail.com Thu May 5 20:56:11 2011 From: ervelazquez <@t> gmail.com (Eric Velazquez) Date: Thu May 5 20:56:17 2011 Subject: [Histonet] ASCP Route 2 HT Certification In-Reply-To: References: <3AD061FE740D464FAC7BF6B5CFB75707E9FC@SMCMAIL01.somerset-healthcare.com> Message-ID: Thank you everyone for your response. I appreciate all your help. -Eric On Thu, May 5, 2011 at 10:58 AM, Fimbres, Amber wrote: > You can try emailing the ASCP, but from my personal experiences (yes, more > than one), I end up getting a reply email saying to call the ASCP directly. > Yeah, not much help when you don't want to wait 1 hour holding on the phone > while trying to work. And I've tried their online chat, it's same issue. > > I may be able to answer a few of your questions as I went through the > process for my HTL exam last year. > > 1.) If you do not qualify for the exam, you (unfortunately) lose your > registration fee. > 2.) However, if you feel like the courses you took just didn't have the > magical 3 and 4 letter words of BIO and CHEM in front of them on your > transcript, you can appeal their decision. I had to do this and I sent them > a bunch of information (evidence) on my courses I took in community college. > I took a 5 unit anatomy course and a 5 unit physiology course with each > class listed on my transcript with the dreaded ANAT abbreviation. However, > if I were to have taken these classes at a 4 year college, it is considered > to be under BIO because it is under their Biology Department. I had to > print from the State Department of Education information on my classes at > the community college and how they correspond to those at a 4 year college. > Luckily in California, we have something called a CAN number for community > college courses to help those that transfer to a 4 year and these are > printed in our college catalogs. I'm lucky my community college keeps their > yearly college catalogs online (even after 8 years!). > 3.) I assume that if you actually take and pass a chemistry course (even > at different schools) that happen to be worded similarly in title, it would > count separately. (You did take each class but that is my assumption). > 4.) No, I don't believe so--you are responsible to make sure you are > qualified. > 5.) I have no idea. > > Good luck! > > Amber Fimbres, MHA, CT(ASCP)HTL > > -----Original Message----- > From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] > Sent: Thursday, May 05, 2011 6:12 AM > To: 'Eric Velazquez'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ASCP Route 2 HT Certification > > You could email them. It would not be so time consuming, and then you would > have proof of an answer should anything be questioned at a later date. Good > luck. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez > Sent: Wednesday, May 04, 2011 5:52 PM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] ASCP Route 2 HT Certification > > Thank you for responding. I agree with you that the ASCP would be the best > source of information, but unfortunately I spoke with someone who was in > charge of reviewing the applications. She was unprofessional and offered > little assistance. I will attempt to contact them again, but finding the > time to be placed on hold for 30mins while I am at work is difficult. I was > hoping someone would know from experience or perhaps someone who is involved > with the process would be able to offer some guidance. > -Eric > > > On Wed, May 4, 2011 at 2:42 PM, Nails, Felton >wrote: > > > Your best answer will come from ASCP so contact them directly. The > > number should be on the application > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez > > Sent: Wednesday, May 04, 2011 4:37 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] ASCP Route 2 HT Certification > > > > Hi, > > > > I was hoping someone could help me with the following questions: > > > > 1) What happens to the money if I were to submit my transcript and my > > fees for the exam and end up not qualifying? Do I lose my money or is > > it credited for when I do qualify? > > 2) How do I determine what classes are considered BIO and Chem? Does > > the prefix for the course ID have to contain BIO or Chem? > > 3) Can the courses taken be similar? Example: School A: I took Chem10: > > Intro to Chemistry & School B: Chm:3 Chemistry basics. If these > > classes are interchangeable between schools do they still count or > > does it have to be a more difficult chemistry course for it to qualify? > > 4)Is there anyway to check if you do qualify before paying exam fees? > > 5)Do you have to pass the class with a C or higher? I took a BIO class > > years ago and received a D, but I was given the credited hours for the > > course. > > > > > > Route 2 for HT certification for ASCP > > At least 60 semester hours (90 quarter hours) of academic credit from > > a regionally accredited college/university, with a combination of 12 > > semester hours (18 quarter hours) of biology and chemistry, or an > > associate degree from a regionally accredited college/university, with > > a combination of 12 semester hours (18 quarter hours) of biology and > > chemistry, AND one year full time acceptable experience in a > > histopathology (clinical, veterinary, industry or research) laboratory > > in the U.S., Canada or an accredited > > laboratory* within the last ten years. > > > > Thanks, > > Eric > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ______________________________________________________________________ > > CONFIDENTIALITY NOTICE: > > The information in this e-mail may be confidential and/or privileged. > > If you are not the intended recipient or an authorized representative > > of the intended recipient, you are hereby notified that any review, > > dissemination, or copying of this e-mail and its attachments, if any, > > or the information contained herein is prohibited. If you have > > received this e-mail in error, please immediately notify the sender > > by return e-mail and delete this e-mail from your computer system. > > Thank you. > > ______________________________________________________________________ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error by > e-mail or you may call Somerset Medical Center's computer Help Desk at > 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, event > listings, health information and more. > > > > This message contains confidential information and is intended only for the > individual named. If you are not the named addressee you should not > disseminate, distribute or copy this e-mail. Please notify the sender > immediately by e-mail if you have received this e-mail by mistake and delete > this e-mail from your system. E-mail transmission cannot be guaranteed to be > secure or error-free as information could be intercepted, corrupted, lost, > destroyed, arrive late or incomplete, or contain viruses. The sender > therefore does not accept liability for any errors or omissions in the > contents of this message, which arise as a result of e-mail transmission. > From lpwenk <@t> sbcglobal.net Thu May 5 21:26:30 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu May 5 21:26:28 2011 Subject: [Histonet] Two Questions In-Reply-To: References: <3439.208.62.167.196.1303913563.squirrel@webmail.realpages.com> Message-ID: Been on vacation (yeah!), so am late responding. This is from the CAP Lab General Checklist. Hope it helps. GEN.71600 Fire Exit Phase II Each room larger than 1000 ft2, or in which major fire hazards exist, has at least 2 exit access doors remote from each other, one of which opens directly into an exit route. REFERENCES 1) Hoeltge GA, et al. Accidental fires in clinical laboratories. Arch Pathol Lab Med. 1993;117:1200-1204 2) National Fire Protection Association. NFPA 45: Standard on Fire Protection for Laboratories Using Chemicals . Quincy, MA: NFPA, 2004 3) National Fire Protection Association. NFPA 99: Standard for Health Care Facilities Chapter 11. Quincy, MA: NFPA, 2005 Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Daniel Schneider" Sent: Wednesday, April 27, 2011 10:24 AM To: "Nicole Tatum" Cc: Subject: Re: [Histonet] Two Questions > On 4/27/11, Nicole Tatum wrote: >> 1st. Does anyone know if there is a rule or law that states a lab must >> have a door? > > I suspect that it's not explicitly spelled out, but I'd caution you, > if you build a lab without doors, you have to make provisions to > provide food and water for the HT's imprisoned inside. It's easier to > put in doors. > > DLS > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Thu May 5 21:27:25 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Thu May 5 21:27:27 2011 Subject: [Histonet] Re: thank you In-Reply-To: <4DC2A2E4.26ED.00D9.1@nehealth.com> References: <4DC2A2E4.26ED.00D9.1@nehealth.com> Message-ID: <59189.12327.qm@web120618.mail.ne1.yahoo.com> Amy - Thank you for your kind words and support. The show is new and the bugs are still being sprayed. The show is actually everyone's show. I encourage anyone interested in being?a guest?to contact me. As you can certainly see, I have folks on who talk?on a wide variety of topics. Not too much on the tech side, for sure.?Just a fun late night or early Monday morning show. It's not meant to be heavy. In two weeks I will?be attending my own state convention in Tampa, FL. I have a list of speakers who I would love to have as guests on the show. I don't want to mention their names and put them on the spot - Ada, Skip, Kaye, Jerry - I meant last names. LOL.?I will have the studio equipment set up (Thanks again Sue Clark and the board) and be doing the interviews right there so attendees can watch and listen if they want. Anyway, thanks again Amy. I appreciate your help in getting the word out. Yours, Dave?? ________________________________ From: Amy Farnan To: histotalk@yahoo.com Cc: litepath2000@yahoo.com Sent: Thu, May 5, 2011 1:15:16 PM Subject: thank you Hi David, Thank you very much! I have to admit I hadn't heard of your show (as I see it is new). I went online last night and looked at your page and listened to your first pod cast. I have to say its wonderful!! I am the editor of our New York?State Newsletter "Onstage" and I will advertise your show in our next issue. I am sure others will be interested in your topics as well! Best of luck to you and thank you again, Kind Regards, Amy Farnan NYSHS Membership Secretary/Newsletter Editor/ Legislative Task Force Chair From rosie_scrimizzi <@t> hotmail.com Thu May 5 22:52:31 2011 From: rosie_scrimizzi <@t> hotmail.com (Rosie Scrimizzi) Date: Thu May 5 22:52:35 2011 Subject: [Histonet] Americlear Staining Problems In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2AC08@EXCHANGE3.huntingtonhospital.com> References: , <57BE698966D5C54EAE8612E8941D76830AD2AC08@EXCHANGE3.huntingtonhospital.com> Message-ID: agree...bleeding eosin is due to water on the slidesEnsure your dehydrating alcohol levels in the stainer containers are a bit higher than your Americlear (to counter evaporation during the day).....if not you will get water contamination of your clearing solution. Rosie ScrimizziTechnical Specialist Siemens (Sakura)Australia > Date: Thu, 5 May 2011 08:17:13 -0700 > From: laurie.colbert@huntingtonhospital.com > To: estellamireles@gmail.com; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Americlear Staining Problems > CC: > > It's usually due to water on the slides. If your alcohols and > Americlear are clean (water-free), check to see that your slides are > totally submerged in these two reagents, and there is not water dripping > down the slide. > Laurie Colbert > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella > Mireles > Sent: Thursday, May 05, 2011 5:50 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Americlear Staining Problems > > Good Morning Histonetters, > Our H&E stained slides are bleeding out the eosin. No, I did not shoot > the > sheriff. > Called the manufacturer, and was advised to: Give more time in the > Alcohols, > Use only 100% Alcohols after eosin. Change the last two Alcohols and > Americlear. Done. Done. Done. > Stain bleeding really improved, but still shows up on hit and miss > cases. > Has anyone had this problem and How did you fix it > > Stella > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mark.Bromley <@t> mps.com.au Thu May 5 23:36:29 2011 From: Mark.Bromley <@t> mps.com.au (Mark Bromley) Date: Thu May 5 23:36:39 2011 Subject: [Histonet] Seeking advice regarding choice of antibody clones. Message-ID: <81F34C5B11F00B4F92A723A2F159167702DBAECD@SIGCOLNT60.sonichealth.com.au> Hello, I'm looking to do some IHC for APC, COX2, bax, bak, TGFbeta, TGFbeta1 and hTERT on FFPE human rectal biopsies, preferably on the Ventana Benchmark platform, and wondered if anyone had experience with these antibodies and could advise the best clones/suppliers. Regards, Mark Bromley. Manager, Histopathology. ___________________________________________ Melbourne Pathology. * 103 Victoria Pde, Melbourne. | '(03) 92877806 www.mps.com.au From Michelle.Perrins <@t> uct.ac.za Fri May 6 08:06:34 2011 From: Michelle.Perrins <@t> uct.ac.za (Michelle Perrins) Date: Fri May 6 08:06:50 2011 Subject: [Histonet] preference: fat stains Message-ID: <4DC40E7A02000070001A3487@gwiasmtp.uct.ac.za> Hello I would like your views on which provides the better result regarding fat stains. 1. fat stains (Oil red O, etc) on frozen sections 2. osmium tetroxide on formalin fixed tissue I know that osmium is nasty to work with but besides that does it produce a better result? Thanks Michelle Michelle Perrins Chief Medical Technologist Forensic Pathology Services Division Forensic Medicine Faculty Health Sciences University of Cape Town tel: +27 21 406 6001 fax: +27 21 448 1249 Email: Michelle.Perrins@uct.ac.za ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### From akbitting <@t> geisinger.edu Fri May 6 08:11:47 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri May 6 08:11:58 2011 Subject: [Histonet] Safety glasses required for embedding In-Reply-To: <361030.45536.qm@web65712.mail.ac4.yahoo.com> References: <4DC2F66E.8060102@yale.edu> <361030.45536.qm@web65712.mail.ac4.yahoo.com> Message-ID: <4DC3BB52.2B7F.00C9.1@geisinger.edu> We went round with a Safety Dept. employee when I first started here about gloves for embedding. Couldn't convince him that we didn't need to wear them. >>> Rene J Buesa 5/5/2011 3:46 PM >>> No. Rern? J. From: Cindy DeRiso To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 5, 2011 3:11 PM Subject: Re: [Histonet] Safety glasses required for embedding I am not sure if this has come up before, but does anyone require personnel to wear safety glasses when embedding and if so is there an OSHA policy? Thanks Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From pruegg <@t> ihctech.net Fri May 6 08:57:10 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri May 6 08:57:15 2011 Subject: [Histonet] ASCP Route 2 HT Certification In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707E9FC@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB75707E9FC@SMCMAIL01.somerset-healthcare.com> Message-ID: Eric, I feel your pain. I too find it difficult to get any help from ASCP, they are happy to take your money for application fees but the customer service is definitely lacking. I have to remember to reup for my QIHC every five years because they send me no notice of it being due, and once I send in my CEU's and $$ I never hear back from them to say they received them and that I am requalified. The only way I know they got it is when the check or cc bill posts to my account. That is no way to be in my opinion. I am sure that your money for applying is an ap fee and will not be returned even if you end up not qualifying to sit for the exam. I had 3 techs get registered in the last couple of years with different levels of education. One had an associates degree with the required number of hours in chemistry and biology. One did not have the AA degree but did have some more advanced courses in chemistry and biology working towards her BS degree and the third has a BS in Biology and has had advance biology and chemistry courses. None of them had any trouble qualifying educationally, and I do not know if they required at least a C in the course, they all had to send their transcripts but they didn't have any problems. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, May 05, 2011 7:12 AM To: 'Eric Velazquez'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Route 2 HT Certification You could email them. It would not be so time consuming, and then you would have proof of an answer should anything be questioned at a later date. Good luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez Sent: Wednesday, May 04, 2011 5:52 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP Route 2 HT Certification Thank you for responding. I agree with you that the ASCP would be the best source of information, but unfortunately I spoke with someone who was in charge of reviewing the applications. She was unprofessional and offered little assistance. I will attempt to contact them again, but finding the time to be placed on hold for 30mins while I am at work is difficult. I was hoping someone would know from experience or perhaps someone who is involved with the process would be able to offer some guidance. -Eric On Wed, May 4, 2011 at 2:42 PM, Nails, Felton wrote: > Your best answer will come from ASCP so contact them directly. The > number should be on the application > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez > Sent: Wednesday, May 04, 2011 4:37 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] ASCP Route 2 HT Certification > > Hi, > > I was hoping someone could help me with the following questions: > > 1) What happens to the money if I were to submit my transcript and my > fees for the exam and end up not qualifying? Do I lose my money or is > it credited for when I do qualify? > 2) How do I determine what classes are considered BIO and Chem? Does > the prefix for the course ID have to contain BIO or Chem? > 3) Can the courses taken be similar? Example: School A: I took Chem10: > Intro to Chemistry & School B: Chm:3 Chemistry basics. If these > classes are interchangeable between schools do they still count or > does it have to be a more difficult chemistry course for it to qualify? > 4)Is there anyway to check if you do qualify before paying exam fees? > 5)Do you have to pass the class with a C or higher? I took a BIO class > years ago and received a D, but I was given the credited hours for the > course. > > > Route 2 for HT certification for ASCP > At least 60 semester hours (90 quarter hours) of academic credit from > a regionally accredited college/university, with a combination of 12 > semester hours (18 quarter hours) of biology and chemistry, or an > associate degree from a regionally accredited college/university, with > a combination of 12 semester hours (18 quarter hours) of biology and > chemistry, AND one year full time acceptable experience in a > histopathology (clinical, veterinary, industry or research) laboratory > in the U.S., Canada or an accredited > laboratory* within the last ten years. > > Thanks, > Eric > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an authorized > representative of the intended recipient, you are hereby notified > that any review, dissemination, or copying of this e-mail and its > attachments, if any, or the information contained herein is > prohibited. If you have received this e-mail in error, please > immediately notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ______________________________________________________________________ > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri May 6 11:37:35 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri May 6 11:37:50 2011 Subject: [Histonet] preference: fat stains In-Reply-To: <4DC40E7A02000070001A3487@gwiasmtp.uct.ac.za> Message-ID: Michelle We use osmium all of the time, we find it works better for image analysis than the Oil Red O. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Perrins Sent: Friday, May 06, 2011 7:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] preference: fat stains Hello I would like your views on which provides the better result regarding fat stains. 1. fat stains (Oil red O, etc) on frozen sections 2. osmium tetroxide on formalin fixed tissue I know that osmium is nasty to work with but besides that does it produce a better result? Thanks Michelle Michelle Perrins Chief Medical Technologist Forensic Pathology Services Division Forensic Medicine Faculty Health Sciences University of Cape Town tel: +27 21 406 6001 fax: +27 21 448 1249 Email: Michelle.Perrins@uct.ac.za ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cdiamond <@t> NSH.org Fri May 6 12:11:45 2011 From: cdiamond <@t> NSH.org (Carrie Diamond) Date: Fri May 6 12:11:50 2011 Subject: [Histonet] RE: NSH Symposium/Convention Registration Message-ID: <06C3A080BBBB71488848280DBC98E17F17DF1C@NSH-SRVR01.nsh.local> Good afternoon - Paper copies of the 37th Annual Symposium/Convention Registration Brochure are currently at the press. Copies will be mailed to all NSH Members within the next two weeks. The complete program is available online through the following link. http://s3.goeshow.com/nsh/annual/2011/schedule_at_a_glance.cfm If you wish to mail your registration form, you can print the official registration form from the link below and send it to the NSH. http://s3.goeshow.com/nsh/annual/2011/PDF/SCRegForm.pdf Please feel free to call the office at 443-535-4060 or email us at histo@nsh.org with any questions. We hope to see you in Cincinnati! Have a great weekend. Carrie Diamond Executive Director National Society for Histotechnology 10320 Little Patuxent Parkway Suite 804 Columbia, MD 21044 P: 443.535.4060 From mfisher <@t> ecrmc.org Fri May 6 12:17:42 2011 From: mfisher <@t> ecrmc.org (Marcia Fisher) Date: Fri May 6 12:17:49 2011 Subject: [Histonet] Safety Glasses during Embedding Message-ID: Please site the reference for this requirement. Thank you. Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center 1415 Ross Ave El Centro, CA 92243 760-339-7267 760-482-5365(F) www.ecrmc.org Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.   From lpwenk <@t> sbcglobal.net Fri May 6 12:48:30 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri May 6 12:48:48 2011 Subject: [Histonet] Safety glasses required for embedding In-Reply-To: <4DC2F66E.8060102@yale.edu> References: <4DC2F66E.8060102@yale.edu> Message-ID: <656890FDF8234376B36146CD5349CFB1@HP2010> OSHA standard for Eye and Face Protection 29CFR1910.133 says that appropriate eye or face protection is required whenever there is a possibility of injury from: - flying particles (includes dust (for histotechs, thinks dry dye powders)) - molten metal - liquid chemicals - acid or caustic liquids - chemical gases or vapors - potentially injurious light radiation (e.g.., UV) (need UV lenses) - processes that produce aerosols of infectious agents OSHA standard for Personal Protective Equipment 29CFR1910.132 says that employers must provide and ensure employees wear PPE whenever there is exposure to hazards that can cause injury, that are: - physical - chemical - biological This could be through inhalation, absorption or physical contact. PPE include for face/head/eyes, extremities, respirators, as needed, in good condition and fit. So safety glasses fall under this standard, also. So unless you have flying particles of tissue hitting your eyes during embedding, or fine mist of infectious aerosols coming off the tissues while embedding, I would say that OSHA does not require safety glasses during embedding. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Cindy DeRiso" Sent: Thursday, May 05, 2011 3:11 PM To: Subject: [Histonet] Safety glasses required for embedding > I am not sure if this has come up before, but does anyone require > personnel to wear safety glasses when embedding and if so is there an OSHA > policy? Thanks > Cindy > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri May 6 13:15:29 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri May 6 13:15:31 2011 Subject: [Histonet] removing MMA Message-ID: <007901cc0c19$947eb7b0$bd7c2710$@northwestern.edu> We have a piece of bone in MMA. The researcher wants us to get It out and do paraffin. Is it possible? What will "melt" the MMA? We also have some 50um slides cut ,that they want to see blood vessels and collagen. Am I going to be able to do an EVG and trichrome or will the plastic inhibit this process? Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu From ratliffjack <@t> hotmail.com Fri May 6 13:49:44 2011 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri May 6 13:49:48 2011 Subject: [Histonet] removing MMA In-Reply-To: <007901cc0c19$947eb7b0$bd7c2710$@northwestern.edu> References: <007901cc0c19$947eb7b0$bd7c2710$@northwestern.edu> Message-ID: Bernice, Let me start by first stating that it is easier to melt away the wax from a paraffin block and then go into resin (MMA), than doing it as you have asked. With that said, it has been my experience that this is very difficult to do, but somewhat possible depending upon what you wish to accomplish. The difficulty is dissolving the interior of the specimen that has been completely infiltrated/polymerized and doing so without disrupting the specimen morphology. In fact, dissolving away the exterior takes a lot of time, patience, and solvent. Additionally, you risk changes in morphology, especially on the surface because now everything is loosely supported and the bone will start to become more brittle due to drying with use of the solvent to deplastify. Now to answer your question simply, "like dissolves like". If you decide to try this, I recommend to use fresh changes of 100% MMA with agitation to help dissolve away the exterior. As the solution becomes thickened from the deplastification, pour out and start again with fresh 100% MMA. You will need to repeat this process SEVERAL times. Take care not to let your dissolving attempts sit too long (overnight in small amount of solvent) or the solution will start to repolymerize. Basically, the resin will become thick in solution as it is dissolving and settle to the bottom. After a prolonged period of time the thicker layers on the bottom with start to repolymerize slowly. Therefore, before you begin I suggest that you first cut and grind away any excess resin to help you reduce both time and money with the amount of MMA solvent you will waste. A note of caution, acetone will also work but it will damage your specimen over time, so I DO NOT recommend using it. Patience and lots of 100% MMA! Regarding the 50um slides, try Sanderson's Rapid Bone Stain (Dorn and Hart Microedge) and counter with Van Gieson picrofuchsin. Let me know if you have any additional questions and feel free to call me (317-281-1975) if you would like to talk over the phone. Best Regards, Jack PS If this project is for Mahesh, tell him I said hello! :) > From: b-frederick@northwestern.edu > To: histonet@lists.utsouthwestern.edu > Date: Fri, 6 May 2011 13:15:29 -0500 > Subject: [Histonet] removing MMA > > We have a piece of bone in MMA. The researcher wants us to get It out and do > paraffin. Is it possible? What will "melt" the MMA? > > We also have some 50um slides cut ,that they want to see blood vessels and > collagen. Am I going to be able to do an EVG and trichrome or will the > plastic inhibit this process? > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > ECOGPCO-RL > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy_McLaughlin <@t> cooley-dickinson.org Fri May 6 14:11:45 2011 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Fri May 6 14:12:03 2011 Subject: [Histonet] Safety glasses required for embedding In-Reply-To: <656890FDF8234376B36146CD5349CFB1@HP2010> References: <4DC2F66E.8060102@yale.edu> <656890FDF8234376B36146CD5349CFB1@HP2010> Message-ID: "Flying particles" can happen during embedding. I have seen molten paraffin splash someone in the eye from opening of the cassette lids. The paraffin then solidified when it hit the eye ball and the eyewash did not wash it out. They had to have it physically removed. This seems to be up for interpretation by the regs. Stacy McLaughlin, HT(ASCP) Lead Histology Technician/Laboratory Safety Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 (413)582-2019 Stacy_McLaughlin@Cooley-Dickinson.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Friday, May 06, 2011 1:49 PM To: cindy.deriso@yale.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Safety glasses required for embedding OSHA standard for Eye and Face Protection 29CFR1910.133 says that appropriate eye or face protection is required whenever there is a possibility of injury from: - flying particles (includes dust (for histotechs, thinks dry dye powders)) - molten metal - liquid chemicals - acid or caustic liquids - chemical gases or vapors - potentially injurious light radiation (e.g.., UV) (need UV lenses) - processes that produce aerosols of infectious agents OSHA standard for Personal Protective Equipment 29CFR1910.132 says that employers must provide and ensure employees wear PPE whenever there is exposure to hazards that can cause injury, that are: - physical - chemical - biological This could be through inhalation, absorption or physical contact. PPE include for face/head/eyes, extremities, respirators, as needed, in good condition and fit. So safety glasses fall under this standard, also. So unless you have flying particles of tissue hitting your eyes during embedding, or fine mist of infectious aerosols coming off the tissues while embedding, I would say that OSHA does not require safety glasses during embedding. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Cindy DeRiso" Sent: Thursday, May 05, 2011 3:11 PM To: Subject: [Histonet] Safety glasses required for embedding > I am not sure if this has come up before, but does anyone require > personnel to wear safety glasses when embedding and if so is there an OSHA > policy? Thanks > Cindy > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Fri May 6 14:19:15 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri May 6 14:19:14 2011 Subject: [Histonet] ASCP Route 2 HT Certification In-Reply-To: References: Message-ID: I'll try to explain what I know, based on working with my students in my NAACLS HT and HTL programs. So I do NOT speak for ASCP BOC (Board of Certification). Don't say "Well, Peggy said so." Doesn't help with my students and ASCP, so won't help with anyone else. (HISTONETTERS: If not interested in ASCP BOC, click DELETE now.) Answers to your Questions: 1. APPLICATION FEE: According to step #3 in the on-line procedure http://www.ascp.org/FunctionalNavigation/certification/GetCertified.aspx And on page 3 of the handbook http://www.ascp.org/pdf/BOR-PDFs/procedures/Examination-Procedures.aspx And on the PDF application form: http://www.ascp.org/pdf/BOR-PDFs/procedures/General-application-form.aspx The application free is non-refundable. (I'll explain why at the bottom of this, if you are interested.) 2. BIO/CHEM COURSES: ASCP will look for BIO or CHEM to see if an applicant took the right courses. But they will also look at the TITLE of the course, knowing there are other majors. Example: MLS = medical laboratory science. AH = Allied health. RT = radiation therapy. And so on - all of which have biology and chemistry courses that would be acceptable. 3. IDENTICAL COURSES: I don't know how "close" the courses have to be, before ASCP BOC won't count them. I would say, if you took a class at college A, then transferred to college B, and college B used that class from college A to say it was the same as one of their (college B) course, so you didn't have to take or pay for the college B course, then I would say that this one course was equal to only 1 course. even though it showed up as two different courses (after all, only one course was attended, exam taken, paid for, etc.). If you took one course at A and one course at B, and both show up as separate courses, then my feeling is that they are two different courses. (A little off to the side, the requirements for HT are 12 semester hours of biology and chemistry combined. That's 1 bio and 2 chem courses (4 credits each), or 2 bio and 1 chem course (4 credits each). NOT 12 credits of bio AND 12 credits of chem. That seems to be a common mistake.) 5. GRADE: I see nothing in the ASCP BOC HT requirements of a minimum grade point. 4. CHECKING BEFORE APPLYING: Now, as to whether there is any way to check before applying and sending in the fee - not really. I appreciate that you are trying to find out if you qualify, before you send in the application, fees and transcript. So the following is not directed towards you, personally. You had some good questions, like, do grades count (since the website doesn't say anything about it). The following is about why, in general, ASCP can't answer individual questions of applicants. Realize that thousands of people apply to the ASCP BOC for all the disciplines, each year. (In 2010, over 12,500 people took the various certification exams.) It is up to the applicant to decide, based on the criteria on-line and in the booklet and application form, if they meet all the criteria to take the exam. ASCP will NOT issue a decision over the phone or by email, before the person applies. If there is a little confusion (such as the 12 credit hours of bio/chem and what does that mean?), they will explain. But they will NOT talk about each person's transcript, work experience, etc. over the phone. They don't have the time. And until they see the transcript, they only have the person's word over the phone as to what the transcript says. And then there is the "but ASCP said over the phone", when maybe they did or didn't say, or there was a misunderstanding on one or both sides. How do you prove what was verbally said? I run a NAACLS HT and HTL program, hospital based, I get lots of people calling up, wanting to know if they qualify. Yet my webpage states very clearly what the criteria is (degree, required courses, GPA, etc.), but lots of people call up, wanting "exceptions". I will not talk about whether their classes meet the criteria or not. If they want this type of information, then they have to submit the application material, pay our programs' $40 application fee, and then I will look over their transcript(s) and will be willing to talk with them afterwards. Most of the time, the people who are calling know that do NOT meet the criteria, but want me spend 30 minutes or more on the phone with them, trying to talk me into reducing my requirements (not needing a degree, only having half the required courses, not having a 3.0 GPA, etc.). They already know they don't meet the criteria. But they want an exception. By my telling them that they have to submit all the required documentation first, with the application fee, before I will decide if they qualify or not, I have reduced the non-qualified people from applying. In the past, over half the applicants who applied to my programs were not qualified. But I would have to take the time to talk with them on the phone, look through and analyze their applications/transcripts, and then write letters of rejection. All of which takes a lot of my time. Once I instituted the non-refundable $40 application fee, and not talking about transcripts over the phone, the number of non-qualified who applied fell significantly. From over 50% to about 10%. With that said, I will talk with someone when they don't understand a requirement on our webpage (e.g., what is a "higher level" biology course?), and I will direct people to the webpage if they haven't been there first. Once they've been to the webpage, if they have more questions, I'll try to answer them (as long as it isn't about their transcript). If someone is really fishing for what they can do with their degree, I will be glad to talk with them about what a histotech is, the advantages/disadvantages of being a histotech, or going through my program, etc. If they are still interested, then it is up to them to go to the webpage, and to figure out if they qualify for the program. I've never kept track, but I still probably talk with about 10 times the number of people, compared with how many people actually apply to my program. But I don't spend 30 minutes with each anymore. I direct them to the criteria on the website, then talk with the ones who call me back. And if they don't want to take the time to read the criteria on my website, well, why would I want them as a student or a histotech? That's my program, with about 15-25 applicants, and 200+ phone calls per year. Now, imagine ASCP BOC and their volume. In 2010, over 12,500 people took the various certification exams. Can you imagine how many people try calling, to find out if ASCP will make an exception? Or will analyze their transcript over the phone? How many people call who will NOT take the exams? Can you see why ASCP BOC can't help all the people calling, trying to decide if they qualify or not? The sheer numbers are overwhelming! So, ASCP BOC is NOT trying to be "unhelpful". They will help to clear up a requirement. But they cannot individually talk with every person about their specific transcript or specific qualification question. There are just too many people wanting exceptions, or who don't want to take the time to read the requirements, or need their hand held during the process. There are just too many people calling every day. Therefore, they handle it by saying - read the criteria, apply, and submit the non-refundable application fee, thus having the applicant be responsible for deciding up front that they meet the criteria. Unfortunately, because of this, when someone is trying to understand one little area, and needs some help, they get caught in the "12,500+" applicant numbers, and maybe end up not getting the help they need. So, Eric, my suggestion: really read the requirements, really look at your transcripts, really look at your experience (since you are going through Route 2). And email Histonet again, with some more of your questions. Peggy A. Wenk, HTL(ASCP)SLS Schools of Histotechnology William Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Eric Velazquez" Sent: Wednesday, May 04, 2011 5:36 PM To: Subject: [Histonet] ASCP Route 2 HT Certification > Hi, > > I was hoping someone could help me with the following questions: > > 1) What happens to the money if I were to submit my transcript and my fees > for the exam and end up not qualifying? Do I lose my money or is it > credited > for when I do qualify? > 2) How do I determine what classes are considered BIO and Chem? Does the > prefix for the course ID have to contain BIO or Chem? > 3) Can the courses taken be similar? Example: School A: I took Chem10: > Intro > to Chemistry & School B: Chm:3 Chemistry basics. If these classes are > interchangeable between schools do they still count or does it have to be > a > more difficult chemistry course for it to qualify? > 4)Is there anyway to check if you do qualify before paying exam fees? > 5)Do you have to pass the class with a C or higher? I took a BIO class > years > ago and received a D, but I was given the credited hours for the course. > > > Route 2 for HT certification for ASCP > At least 60 semester hours (90 quarter hours) of academic credit from a > regionally accredited college/university, with a combination of 12 > semester > hours (18 quarter hours) of biology and chemistry, or an associate degree > from a regionally accredited college/university, with a combination of 12 > semester hours (18 quarter hours) of biology and chemistry, AND one year > full time acceptable experience in a histopathology (clinical, veterinary, > industry or research) laboratory in the U.S., Canada or an accredited > laboratory* within the last ten years. > > Thanks, > Eric > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Fri May 6 14:52:26 2011 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri May 6 14:52:36 2011 Subject: [Histonet] removing MMA In-Reply-To: <007901cc0c19$947eb7b0$bd7c2710$@northwestern.edu> References: <007901cc0c19$947eb7b0$bd7c2710$@northwestern.edu> Message-ID: <4DC4517A.60804@shaw.ca> Years ago when I had to remove plexiglass rods from eyes, I used chloroform. It takes a few days, but methyl methacrylate dissolves in chloroform quite well. In fact, plexiglass containers for medical museum specimens are cemented together with MMA dissolved in chloroform. Bryan Llewellyn Bernice Frederick wrote: > We have a piece of bone in MMA. The researcher wants us to get It out and do > paraffin. Is it possible? What will "melt" the MMA? > > We also have some 50um slides cut ,that they want to see blood vessels and > collagen. Am I going to be able to do an EVG and trichrome or will the > plastic inhibit this process? > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > ECOGPCO-RL > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bob.nienhuis <@t> gmail.com Fri May 6 16:21:06 2011 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Fri May 6 16:21:11 2011 Subject: [Histonet] removing MMA In-Reply-To: <007901cc0c19$947eb7b0$bd7c2710$@northwestern.edu> References: <007901cc0c19$947eb7b0$bd7c2710$@northwestern.edu> Message-ID: We use acetone to dissolve MMA from implants. Don't know what that would do to tissue. Bob On Fri, May 6, 2011 at 11:15 AM, Bernice Frederick < b-frederick@northwestern.edu> wrote: > We have a piece of bone in MMA. The researcher wants us to get It out and > do > paraffin. Is it possible? What will "melt" the MMA? > > We also have some 50um slides cut ,that they want to see blood vessels and > collagen. Am I going to be able to do an EVG and trichrome or will the > plastic inhibit this process? > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > ECOGPCO-RL > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dlaudier <@t> gmail.com Fri May 6 16:26:57 2011 From: dlaudier <@t> gmail.com (Damien Laudier) Date: Fri May 6 16:27:01 2011 Subject: [Histonet] Re: Removing MMA Message-ID: Hi Bernice, If you have any intentions of putting this sample on an enclosed tissue processor for paraffin infiltration, after methacrylate removal, you must remove every trace of methyl methacrylate monomer. Traces (even small traces) of methacrylate monomer can wreck havoc on most enclosed tissue processor solvent-transfer lines and pump gaskets; you could end up with a very expensive repair bill. For transferring into paraffin, you?re better off treating these samples as you would treat a section you wish to deplasticize. Assuming your sample was properly fixed and processed from the beginning, xylene or toluene will do the job of breaking down the polymerized methacrylate just fine. Once the trimmed-down block has dissolved down, several more changes are required to remove the methacrylate and avoid potential carryover danger. As an additional safeguard, I would take the sample back to 200 proof ethanol or 100% reagent ethanol for a couple of changes & then start the paraffin infiltration process with the last ethanol step on your processor. If you need to decalcify this sample, then you can just continue on back to water and start from the beginning. Yes, you can do any tinctorial stain on your ground sections if etched well beforehand. -Damien L. From shrivastavaanuradha <@t> hotmail.com Fri May 6 16:34:29 2011 From: shrivastavaanuradha <@t> hotmail.com (anuradha shrivastava) Date: Fri May 6 16:34:29 2011 Subject: [Histonet] hematoxylin washed off Message-ID: Hello Every body, We changed the solutions testerday in the processor, and today Dr. complained about pale hematoxylin. Can u suggest what is wrong. thanks. anu. From histonet.nospam <@t> vneubert.com Sat May 7 01:42:22 2011 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Sat May 7 01:42:32 2011 Subject: [Histonet] hematoxylin washed off In-Reply-To: References: Message-ID: <4DC4E9CE.30103@vneubert.com> Is it only the HTX which is pale? Did you change anything else? Like dewaxing? anuradha shrivastava: > Hello Every body, > We changed the solutions testerday in the processor, and today Dr. complained about pale hematoxylin. Can u suggest what is wrong. > thanks. > anu. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Sat May 7 06:35:11 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat May 7 06:35:30 2011 Subject: [Histonet] hematoxylin washed off In-Reply-To: <4DC4E9CE.30103@vneubert.com> References: <4DC4E9CE.30103@vneubert.com> Message-ID: <967ACDDF-855D-4600-87A7-2BAEF04AF6F6@imagesbyhopper.com> We had a similar thing happen last week, except in our case it was the eosin was very pale & the hematoxylin was more blue than purple. Through our troubleshooting steps, we determined it could have only been the processor reagents. We fully changed it and the problem went away. While I am delighted that the staining has returned to normal, I have no idea what really happened. Here are some of my first thoughts: Have you checked your reagents on the stainer? Same lot numbers, differerent lot number? Do you use progressive or regressive technique? If regressive, are your differentiating/bluing reagents new since the processor change? Did you use new/different reagents on the processor? Did you wash the bottles/dishes with a cleaning solution that, maybe, didn't get fully rinsed out? Or do the dishes need stronger cleaning? Michelle Sent from my iPhone On May 7, 2011, at 2:42 AM, "V. Neubert" wrote: > Is it only the HTX which is pale? Did you change anything else? Like > dewaxing? > > anuradha shrivastava: >> Hello Every body, >> We changed the solutions testerday in the processor, and today Dr. complained about pale hematoxylin. Can u suggest what is wrong. >> thanks. >> anu. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From hrumbut <@t> yahoo.com Sat May 7 09:50:11 2011 From: hrumbut <@t> yahoo.com (Heather R) Date: Sat May 7 09:50:17 2011 Subject: [Histonet] Procedure Manual Template Message-ID: <851825.97583.qm@web130204.mail.mud.yahoo.com> Does anyone know of a standard operating procedure manual template could be found?? I have to write one and need a little guidance. And thanks to all who have helped with my other questions, I cant figure out how to reply to the old posts... Thanks again Heather From nelsonrnch <@t> verizon.net Sat May 7 10:00:01 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Sat May 7 10:00:07 2011 Subject: [Histonet] Procedure Manual Template Message-ID: <718642.41680.qm@web84302.mail.re1.yahoo.com> Heather I feel your pain, Please contact me on my personal e-mail. I can try to help. I had to write my own procedure manuals also. THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net From syvette04 <@t> aol.com Sat May 7 21:16:21 2011 From: syvette04 <@t> aol.com (Yvette) Date: Sat May 7 21:16:43 2011 Subject: [Histonet] Is anybody hiring techs without a certification Message-ID: <37530149-F9EA-4FF4-B6DE-7CA38BBF5F8C@aol.com> I'm looking for a histo job (HT) but my eligibility to test has expired. I became a tech through AFIP in 01. Sent from my iPhone From rsrichmond <@t> gmail.com Sun May 8 12:38:38 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sun May 8 12:38:43 2011 Subject: [Histonet] Re: Is anybody hiring techs without a certification? Message-ID: Yvette asks: >>Is anybody hiring techs without a certification?<< Is anybody hiring techs WITH a certification? With a coming retirement, the two labs I'm working in now will have NO certified histotechs (total of four workers) - all OJT's. Some of them probably have the educational qualifications to sit the exam, but management isn't very sympathetic - I'll have to buy them a copy of the 3rd edition of Freida Carson's book, for starters. Bob Richmond Samurai Pathologist Knoxville TN From mucram11 <@t> comcast.net Sun May 8 16:33:28 2011 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Sun May 8 16:33:32 2011 Subject: [Histonet] Re: Is anybody hiring techs without a certification? In-Reply-To: <1971126660.46198.1304889988173.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <1906175750.46291.1304890408579.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Currently we have too few schools and not enough slots in the schools we have for Histology to grow ans educate people properly.? It is hard to tell if the ASCP and NSH are just unaware of the issues or not interested as we see schools close and career days at NSH for high school students yet no real action.???They need to assist?what many of us are doing now - OJT with people who have the AA or AS degree and hope they stay in the field.? The majority of people I meet and tell what I do have no idea that the field of Histology exists or how important it can be to a patient having surgery and awaiting a diagnosis.? I currently have two people we are training as we?can't find qualified people here and at least they are the best we could find.? The second issue is pay and the fact that any?Histology area controlled by Clinical Laboratory is in an uphill battle to get?salaries raised as the CP people all seem to feel if you?don't have a BS you can't rate better pay.? I do realize we have many? MTs in Histology and they usually great?for us.? It is the management that cannot get by the requirements to help us go forward.? They should look at the fact many of the Histologist now working will retire in the next ten years and then where will they be in finding people for this field? Pam Marcum Personal Option ----- Original Message ----- From: "Robert Richmond" < rsrichmond @ gmail .com> To: histonet @lists. utsouthwestern . edu Sent: Sunday, May 8, 2011 12:38:38 PM Subject: [ Histonet ] Re: Is anybody hiring techs without a certification? Yvette asks: >>Is anybody hiring techs without a certification?<< Is anybody hiring techs WITH a certification? With a coming retirement, the two labs I'm working in now will have NO certified histotechs (total of four workers) - all OJT's . Some of them probably have the educational qualifications to sit the exam, but management isn't very sympathetic - I'll have to buy them a copy of the 3rd edition of Freida Carson's book, for starters. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet From sadey <@t> hotmail.ca Sun May 8 17:57:05 2011 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Sun May 8 17:57:08 2011 Subject: [Histonet] Biocare Intelipath Message-ID: Hello Netters. We are considering an Intelipath. I'd like some opinions on the instrument from users out there. Thanks in advance!!! Sheila From JMacDonald <@t> mtsac.edu Sun May 8 18:07:34 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun May 8 18:07:40 2011 Subject: [Histonet] Re: Is anybody hiring techs without a certification? In-Reply-To: <1906175750.46291.1304890408579.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: The NSH and ASCP are well aware of the lack of schools, but are powerless to do anything about it. With cutbacks in education and healthcare schools and hospitals are cutting back on training as it takes time and money. What type of assistance are you speaking about for the OJT people who have the AA/AS? The ASCP recognizes these individuals through route 2 for the HT (ASCP) exam. One of the reasons that our program and others do not take more students is that we cannot find facilities that are willing to take on the clinical experience for students. Many of the histology labs are unwilling or unable to train students. What type of action would you like the NSH and ASCP to take? Jennifer MacDonald Pamela Marcum Sent by: histonet-bounces@lists.utsouthwestern.edu 05/08/2011 02:35 PM To Robert Richmond cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Re: Is anybody hiring techs without a certification? Currently we have too few schools and not enough slots in the schools we have for Histology to grow ans educate people properly. It is hard to tell if the ASCP and NSH are just unaware of the issues or not interested as we see schools close and career days at NSH for high school students yet no real action. They need to assist what many of us are doing now - OJT with people who have the AA or AS degree and hope they stay in the field. The majority of people I meet and tell what I do have no idea that the field of Histology exists or how important it can be to a patient having surgery and awaiting a diagnosis. I currently have two people we are training as we can't find qualified people here and at least they are the best we could find. The second issue is pay and the fact that any Histology area controlled by Clinical Laboratory is in an uphill battle to get salaries raised as the CP people all seem to feel if you don't have a BS you can't rate better pay. I do realize we have many MTs in Histology and they usually great for us. It is the management that cannot get by the requirements to help us go forward. They should look at the fact many of the Histologist now working will retire in the next ten years and then where will they be in finding people for this field? Pam Marcum Personal Option ----- Original Message ----- From: "Robert Richmond" < rsrichmond @ gmail .com> To: histonet @lists. utsouthwestern . edu Sent: Sunday, May 8, 2011 12:38:38 PM Subject: [ Histonet ] Re: Is anybody hiring techs without a certification? Yvette asks: >>Is anybody hiring techs without a certification?<< Is anybody hiring techs WITH a certification? With a coming retirement, the two labs I'm working in now will have NO certified histotechs (total of four workers) - all OJT's . Some of them probably have the educational qualifications to sit the exam, but management isn't very sympathetic - I'll have to buy them a copy of the 3rd edition of Freida Carson's book, for starters. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Sun May 8 19:11:14 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun May 8 19:11:27 2011 Subject: [Histonet] preference: fat stains In-Reply-To: <4DC40E7A02000070001A3487@gwiasmtp.uct.ac.za> References: <4DC40E7A02000070001A3487@gwiasmtp.uct.ac.za> Message-ID: <6D6BD1DE8A5571489398B392A38A71571885470E@xmdb02.nch.kids> Definitely go with Oil Red O rather than Osmium. Osmium tetroxide reacts with unsaturated lipids whereas ORO colours neutral lipids. You can do an ORO on formalin fixed tissue (frozen sections). You could also look at the Sudan Black techniques. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Perrins Sent: Friday, 6 May 2011 11:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] preference: fat stains Hello I would like your views on which provides the better result regarding fat stains. 1. fat stains (Oil red O, etc) on frozen sections 2. osmium tetroxide on formalin fixed tissue I know that osmium is nasty to work with but besides that does it produce a better result? Thanks Michelle Michelle Perrins Chief Medical Technologist Forensic Pathology Services Division Forensic Medicine Faculty Health Sciences University of Cape Town tel: +27 21 406 6001 fax: +27 21 448 1249 Email: Michelle.Perrins@uct.ac.za ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From mucram11 <@t> comcast.net Sun May 8 19:31:00 2011 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Sun May 8 19:31:08 2011 Subject: [Histonet] Re: Is anybody hiring techs without a certification? In-Reply-To: Message-ID: <1163358151.49110.1304901060488.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Jennifer, I am not sure you understood that I know the paths to getting registration.? We can argue this forever however;?the outcome is the same too little; too late for Histology to grow.? No one in?a Med Tech program since the middle 60's?is required to even set foot in Histology so we are the not known and will not be until we or one of these organizations goes beyond lip?service and promises.??After 40 years I am still asked what kind of history I teach.? I explain what we do and generally hear of that's great, never knew you guys existed.? I go to schools when I can someone to invite me and still it is not a field anyone knows. ??We currently have two people training in our lab.? One has an AA and the other an AS degree.? Both are taking additional science and chemistry courses to help them understand?what they are being taught.? I am the person in charge of the Histology Lab, the Gross Room and the Morgue.? So, Yes I know the paths for all three areas.? Unfortunately, the ASCP and NSH are aware and still when there is a meeting for CLMA , ASCP or other areas of the laboratory you will be lucky to find a lecture on anything to do with Histology whether local or national.? No funds are available for anyone to go to a meeting or travel.??IF NSH is not local most people can't get there.? So exactly where are we to get education CEUs if no one will pay for them and we are at the lowest end of the pay scale and can't?? Most of the?Histologist here are unable to travel more than 10 to 50 miles for a meeting even in the state.?? We are at a University and have been told point blank no education money (time off only), no classes unless they are free and oh by the way be sure the new ones get their CEUs to keep their registries.? We have also been told anyone without a BS will be paid like a LST and the HT does not qualify them for a raise or higher pay.? I have friends at other major institutes who are being told the same thing.? We ask in the early 90's for the education levels to be raised by NSH and still took years to happen.? I have been in Histology for over 40 years and still love the field, just hate the way we are treated in comparison to the CP area.? Try hiring someone under the new guidelines for grossing without a PA license and see what you get.? It is just as hard and just a few schools for that field too.?? When you get the PA license try getting the pay outside a major metropolitan area. Pam Marcum Still my opinion ----- Original Message ----- From: "Jennifer MacDonald" < JMacDonald @ mtsac . edu > To: "Pamela Marcum" Cc: histonet @lists. utsouthwestern . edu , histonet -bounces@lists. utsouthwestern . edu , "Robert Richmond" < rsrichmond @ gmail .com> Sent: Sunday, May 8, 2011 6:07:34 PM Subject: Re: [ Histonet ] Re: Is anybody hiring techs without a certification? The NSH and ASCP are well aware of the lack of schools, but are powerless to do anything about it. ? ?With cutbacks in education and healthcare schools and hospitals are cutting back on training as it takes time and money. ?What type of assistance are you speaking about for the OJT people who have the AA/AS? ?The ASCP recognizes these individuals through route 2 for the HT ( ASCP ) exam. One of the reasons that our program and others do not take more students is that we cannot find facilities that are willing to take on the clinical experience for students. ?Many of the histology labs are unwilling or unable to train students. ?What type of action would you like the NSH and ASCP to take? Jennifer MacDonald ----- Original Message ----- From: "Jennifer MacDonald" < JMacDonald @ mtsac . edu > To: "Pamela Marcum" Cc: histonet @lists. utsouthwestern . edu , histonet -bounces@lists. utsouthwestern . edu , "Robert Richmond" < rsrichmond @ gmail .com> Sent: Sunday, May 8, 2011 6:07:34 PM Subject: Re: [ Histonet ] Re: Is anybody hiring techs without a certification? The NSH and ASCP are well aware of the lack of schools, but are powerless to do anything about it. ? ?With cutbacks in education and healthcare schools and hospitals are cutting back on training as it takes time and money. ?What type of assistance are you speaking about for the OJT people who have the AA/AS? ?The ASCP recognizes these individuals through route 2 for the HT ( ASCP ) exam. One of the reasons that our program and others do not take more students is that we cannot find facilities that are willing to take on the clinical experience for students. ?Many of the histology labs are unwilling or unable to train students. ?What type of action would you like the NSH and ASCP to take? Jennifer MacDonald Pamela Marcum Sent by: histonet -bounces@lists. utsouthwestern . edu 05/08/2011 02:35 PM To Robert Richmond < rsrichmond @ gmail .com> cc histonet @lists. utsouthwestern . edu Subject Re: [ Histonet ] Re: Is anybody hiring techs without a certification? Currently we have too few schools and not enough slots in the schools we have for Histology to grow ans educate people properly.? It is hard to tell if the ASCP and NSH are just unaware of the issues or not interested as we see schools close and career days at NSH for high school students yet no real action.???They need to assist?what many of us are doing now - OJT with people who have the AA or AS degree and hope they stay in the field.? The majority of people I meet and tell what I do have no idea that the field of Histology exists or how important it can be to a patient having surgery and awaiting a diagnosis.? I currently have two people we are training as we?can't find qualified people here and at least they are the best we could find.? The second issue is pay and the fact that any?Histology area controlled by Clinical Laboratory is in an uphill battle to get?salaries raised as the CP people all seem to feel if you?don't have a BS you can't rate better pay.? I do realize we have many? MTs in Histology and they usually great?for us.? It is the management that cannot get by the requirements to help us go forward.? They should look at the fact many of the Histologist now working will retire in the next ten years and then where will they be in finding people for this field? Pam Marcum Personal Option ----- Original Message ----- From: "Robert Richmond" < rsrichmond @ gmail .com> To: histonet @lists. utsouthwestern . edu Sent: Sunday, May 8, 2011 12:38:38 PM Subject: [ Histonet ] Re: Is anybody hiring techs without a certification? Yvette asks: >>Is anybody hiring techs without a certification?<< Is anybody hiring techs WITH a certification? With a coming retirement, the two labs I'm working in now will have NO certified histotechs (total of four workers) - all OJT's . Some of them probably have the educational qualifications to sit the exam, but management isn't very sympathetic - I'll have to buy them a copy of the 3rd edition of Freida Carson's book, for starters. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet From JMacDonald <@t> mtsac.edu Sun May 8 20:30:23 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun May 8 20:35:45 2011 Subject: [Histonet] Re: Is anybody hiring techs without a certification? In-Reply-To: <1163358151.49110.1304901060488.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: Pamela, Thank you for clarifying. This is not what I understood from you first post. Jennifer Pamela Marcum Sent by: histonet-bounces@lists.utsouthwestern.edu 05/08/2011 05:32 PM To Jennifer MacDonald cc histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu, Robert Richmond Subject Re: [Histonet] Re: Is anybody hiring techs without a certification? Jennifer, I am not sure you understood that I know the paths to getting registration. We can argue this forever however; the outcome is the same too little; too late for Histology to grow. No one in a Med Tech program since the middle 60's is required to even set foot in Histology so we are the not known and will not be until we or one of these organizations goes beyond lip service and promises. After 40 years I am still asked what kind of history I teach. I explain what we do and generally hear of that's great, never knew you guys existed. I go to schools when I can someone to invite me and still it is not a field anyone knows. We currently have two people training in our lab. One has an AA and the other an AS degree. Both are taking additional science and chemistry courses to help them understand what they are being taught. I am the person in charge of the Histology Lab, the Gross Room and the Morgue. So, Yes I know the paths for all three areas. Unfortunately, the ASCP and NSH are aware and still when there is a meeting for CLMA , ASCP or other areas of the laboratory you will be lucky to find a lecture on anything to do with Histology whether local or national. No funds are available for anyone to go to a meeting or travel. IF NSH is not local most people can't get there. So exactly where are we to get education CEUs if no one will pay for them and we are at the lowest end of the pay scale and can't? Most of the Histologist here are unable to travel more than 10 to 50 miles for a meeting even in the state. We are at a University and have been told point blank no education money (time off only), no classes unless they are free and oh by the way be sure the new ones get their CEUs to keep their registries. We have also been told anyone without a BS will be paid like a LST and the HT does not qualify them for a raise or higher pay. I have friends at other major institutes who are being told the same thing. We ask in the early 90's for the education levels to be raised by NSH and still took years to happen. I have been in Histology for over 40 years and still love the field, just hate the way we are treated in comparison to the CP area. Try hiring someone under the new guidelines for grossing without a PA license and see what you get. It is just as hard and just a few schools for that field too. When you get the PA license try getting the pay outside a major metropolitan area. Pam Marcum Still my opinion ----- Original Message ----- From: "Jennifer MacDonald" < JMacDonald @ mtsac . edu > To: "Pamela Marcum" Cc: histonet @lists. utsouthwestern . edu , histonet -bounces@lists. utsouthwestern . edu , "Robert Richmond" < rsrichmond @ gmail .com> Sent: Sunday, May 8, 2011 6:07:34 PM Subject: Re: [ Histonet ] Re: Is anybody hiring techs without a certification? The NSH and ASCP are well aware of the lack of schools, but are powerless to do anything about it. With cutbacks in education and healthcare schools and hospitals are cutting back on training as it takes time and money. What type of assistance are you speaking about for the OJT people who have the AA/AS? The ASCP recognizes these individuals through route 2 for the HT ( ASCP ) exam. One of the reasons that our program and others do not take more students is that we cannot find facilities that are willing to take on the clinical experience for students. Many of the histology labs are unwilling or unable to train students. What type of action would you like the NSH and ASCP to take? Jennifer MacDonald ----- Original Message ----- From: "Jennifer MacDonald" < JMacDonald @ mtsac . edu > To: "Pamela Marcum" Cc: histonet @lists. utsouthwestern . edu , histonet -bounces@lists. utsouthwestern . edu , "Robert Richmond" < rsrichmond @ gmail .com> Sent: Sunday, May 8, 2011 6:07:34 PM Subject: Re: [ Histonet ] Re: Is anybody hiring techs without a certification? The NSH and ASCP are well aware of the lack of schools, but are powerless to do anything about it. With cutbacks in education and healthcare schools and hospitals are cutting back on training as it takes time and money. What type of assistance are you speaking about for the OJT people who have the AA/AS? The ASCP recognizes these individuals through route 2 for the HT ( ASCP ) exam. One of the reasons that our program and others do not take more students is that we cannot find facilities that are willing to take on the clinical experience for students. Many of the histology labs are unwilling or unable to train students. What type of action would you like the NSH and ASCP to take? Jennifer MacDonald Pamela Marcum Sent by: histonet -bounces@lists. utsouthwestern . edu 05/08/2011 02:35 PM To Robert Richmond < rsrichmond @ gmail .com> cc histonet @lists. utsouthwestern . edu Subject Re: [ Histonet ] Re: Is anybody hiring techs without a certification? Currently we have too few schools and not enough slots in the schools we have for Histology to grow ans educate people properly. It is hard to tell if the ASCP and NSH are just unaware of the issues or not interested as we see schools close and career days at NSH for high school students yet no real action. They need to assist what many of us are doing now - OJT with people who have the AA or AS degree and hope they stay in the field. The majority of people I meet and tell what I do have no idea that the field of Histology exists or how important it can be to a patient having surgery and awaiting a diagnosis. I currently have two people we are training as we can't find qualified people here and at least they are the best we could find. The second issue is pay and the fact that any Histology area controlled by Clinical Laboratory is in an uphill battle to get salaries raised as the CP people all seem to feel if you don't have a BS you can't rate better pay. I do realize we have many MTs in Histology and they usually great for us. It is the management that cannot get by the requirements to help us go forward. They should look at the fact many of the Histologist now working will retire in the next ten years and then where will they be in finding people for this field? Pam Marcum Personal Option ----- Original Message ----- From: "Robert Richmond" < rsrichmond @ gmail .com> To: histonet @lists. utsouthwestern . edu Sent: Sunday, May 8, 2011 12:38:38 PM Subject: [ Histonet ] Re: Is anybody hiring techs without a certification? Yvette asks: >>Is anybody hiring techs without a certification?<< Is anybody hiring techs WITH a certification? With a coming retirement, the two labs I'm working in now will have NO certified histotechs (total of four workers) - all OJT's . Some of them probably have the educational qualifications to sit the exam, but management isn't very sympathetic - I'll have to buy them a copy of the 3rd edition of Freida Carson's book, for starters. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Mon May 9 10:07:39 2011 From: mward <@t> wfubmc.edu (Martha Ward) Date: Mon May 9 10:08:03 2011 Subject: [Histonet] Processing hair Message-ID: I am posting this question for a colleague who has been approached to do a research project involving processing human hair. Has anyone done this, and if so, could you provide the procedure. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 From Ronald.Houston <@t> nationwidechildrens.org Mon May 9 10:51:43 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon May 9 10:51:50 2011 Subject: [Histonet] Microprobe Message-ID: Looking for a new or used Fisher Microprobe. Please contact me off-line if you can help Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From algranth <@t> email.arizona.edu Mon May 9 10:58:34 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon May 9 10:58:45 2011 Subject: [Histonet] Re: Is anybody hiring techs without a certification? In-Reply-To: <1163358151.49110.1304901060488.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <1163358151.49110.1304901060488.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <54A12FE0-A43A-46EE-8A45-E4060C16853E@email.arizona.edu> Pam, I can tell you that NSH is totally aware of this situation and one of our goals is to try to do something about it. These things are not done quickly however but little by little we are making some progress. We have partnered with other healthcare professionals like CLSI, NAACLS, CLMA, Health Professions Network, Assoc. of Pathology Chairs just to name a few (for a better list check out the NSH web site). All this in an effort to make histotechnology and histotechs known in the healthcare community. Programs like the Career Days and the Histotechnology Professionals Day were created for this purpose as well. These things don't happen by themselves either, we all have to take the responsibility of getting the word out. I know that you are doing what you can - I am too, speaking at local schools and service clubs. For HPD I had an article in our local newspaper. We just need more techs willing to do these things. When techs can't travel to an educational venue there are teleconferences, the online learning center ( especially for your OJT's) and CEU's from the Journal just to name some alternatives. We do need more schools but this is difficult. We had a very successful program here at our Community College but (and because of lack of understanding and some political stuff too) they discontinued it last year. Andi Grantham On May 8, 2011, at 5:31 PM, Pamela Marcum wrote: > > > Jennifer, > > > > I am not sure you understood that I know the paths to getting registration. We can argue this forever however; the outcome is the same too little; too late for Histology to grow. No one in a Med Tech program since the middle 60's is required to even set foot in Histology so we are the not known and will not be until we or one of these organizations goes beyond lip service and promises. After 40 years I am still asked what kind of history I teach. I explain what we do and generally hear of that's great, never knew you guys existed. I go to schools when I can someone to invite me and still it is not a field anyone knows. > > > > We currently have two people training in our lab. One has an AA and the other an AS degree. Both are taking additional science and chemistry courses to help them understand what they are being taught. I am the person in charge of the Histology Lab, the Gross Room and the Morgue. So, Yes I know the paths for all three areas. Unfortunately, the ASCP and NSH are aware and still when there is a meeting for CLMA , ASCP or other areas of the laboratory you will be lucky to find a lecture on anything to do with Histology whether local or national. No funds are available for anyone to go to a meeting or travel. IF NSH is not local most people can't get there. So exactly where are we to get education CEUs if no one will pay for them and we are at the lowest end of the pay scale and can't? Most of the Histologist here are unable to travel more than 10 to 50 miles for a meeting even in the state. > > > > We are at a University and have been told point blank no education money (time off only), no classes unless they are free and oh by the way be sure the new ones get their CEUs to keep their registries. We have also been told anyone without a BS will be paid like a LST and the HT does not qualify them for a raise or higher pay. I have friends at other major institutes who are being told the same thing. We ask in the early 90's for the education levels to be raised by NSH and still took years to happen. I have been in Histology for over 40 years and still love the field, just hate the way we are treated in comparison to the CP area. Try hiring someone under the new guidelines for grossing without a PA license and see what you get. It is just as hard and just a few schools for that field too. When you get the PA license try getting the pay outside a major metropolitan area. > > > > Pam Marcum > > Still my opinion > > > > > > > > > > ----- Original Message ----- > From: "Jennifer MacDonald" < JMacDonald @ mtsac . edu > > To: "Pamela Marcum" > Cc: histonet @lists. utsouthwestern . edu , histonet -bounces@lists. utsouthwestern . edu , "Robert Richmond" < rsrichmond @ gmail .com> > Sent: Sunday, May 8, 2011 6:07:34 PM > Subject: Re: [ Histonet ] Re: Is anybody hiring techs without a certification? > > > The NSH and ASCP are well aware of the lack of schools, but are powerless to do anything about it. With cutbacks in education and healthcare schools and hospitals are cutting back on training as it takes time and money. What type of assistance are you speaking about for the OJT people who have the AA/AS? The ASCP recognizes these individuals through route 2 for the HT ( ASCP ) exam. > One of the reasons that our program and others do not take more students is that we cannot find facilities that are willing to take on the clinical experience for students. Many of the histology labs are unwilling or unable to train students. What type of action would you like the NSH and ASCP to take? > > Jennifer MacDonald > > > > > > > > > > ----- Original Message ----- > From: "Jennifer MacDonald" < JMacDonald @ mtsac . edu > > To: "Pamela Marcum" > Cc: histonet @lists. utsouthwestern . edu , histonet -bounces@lists. utsouthwestern . edu , "Robert Richmond" < rsrichmond @ gmail .com> > Sent: Sunday, May 8, 2011 6:07:34 PM > Subject: Re: [ Histonet ] Re: Is anybody hiring techs without a certification? > > > The NSH and ASCP are well aware of the lack of schools, but are powerless to do anything about it. With cutbacks in education and healthcare schools and hospitals are cutting back on training as it takes time and money. What type of assistance are you speaking about for the OJT people who have the AA/AS? The ASCP recognizes these individuals through route 2 for the HT ( ASCP ) exam. > One of the reasons that our program and others do not take more students is that we cannot find facilities that are willing to take on the clinical experience for students. Many of the histology labs are unwilling or unable to train students. What type of action would you like the NSH and ASCP to take? > > Jennifer MacDonald > > > > Pamela Marcum > Sent by: histonet -bounces@lists. utsouthwestern . edu > > 05/08/2011 02:35 PM > To Robert Richmond < rsrichmond @ gmail .com> > > cc histonet @lists. utsouthwestern . edu > > Subject Re: [ Histonet ] Re: Is anybody hiring techs without a certification? > > > > > > > Currently we have too few schools and not enough slots in the schools we have for Histology to grow ans educate people properly. It is hard to tell if the ASCP and NSH are just unaware of the issues or not interested as we see schools close and career days at NSH for high school students yet no real action. They need to assist what many of us are doing now - OJT with people who have the AA or AS degree and hope they stay in the field. The majority of people I meet and tell what I do have no idea that the field of Histology exists or how important it can be to a patient having surgery and awaiting a diagnosis. > > > > I currently have two people we are training as we can't find qualified people here and at least they are the best we could find. The second issue is pay and the fact that any Histology area controlled by Clinical Laboratory is in an uphill battle to get salaries raised as the CP people all seem to feel if you don't have a BS you can't rate better pay. I do realize we have many MTs in Histology and they usually great for us. It is the management that cannot get by the requirements to help us go forward. They should look at the fact many of the Histologist now working will retire in the next ten years and then where will they be in finding people for this field? > > > > Pam Marcum > > Personal Option > > > > > > > > ----- Original Message ----- > From: "Robert Richmond" < rsrichmond @ gmail .com> > To: histonet @lists. utsouthwestern . edu > Sent: Sunday, May 8, 2011 12:38:38 PM > Subject: [ Histonet ] Re: Is anybody hiring techs without a certification? > > Yvette asks: > >>> Is anybody hiring techs without a certification?<< > > Is anybody hiring techs WITH a certification? With a coming > retirement, the two labs I'm working in now will have NO certified > histotechs (total of four workers) - all OJT's . Some of them probably > have the educational qualifications to sit the exam, but management > isn't very sympathetic - I'll have to buy them a copy of the 3rd > edition of Freida Carson's book, for starters. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet @lists. utsouthwestern . edu > http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet > _______________________________________________ > Histonet mailing list > Histonet @lists. utsouthwestern . edu > http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jkrupp <@t> deltacollege.edu Mon May 9 11:26:45 2011 From: jkrupp <@t> deltacollege.edu (Jon Krupp) Date: Mon May 9 11:26:53 2011 Subject: [Histonet] Re: Is anybody hiring techs without a certification? In-Reply-To: <54A12FE0-A43A-46EE-8A45-E4060C16853E@email.arizona.edu> References: <1163358151.49110.1304901060488.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> <54A12FE0-A43A-46EE-8A45-E4060C16853E@email.arizona.edu> Message-ID: <9BB50FCA-EE57-42EB-846E-404130B3C26B@deltacollege.edu> On May 9, 2011, at 8:58 AM, Grantham, Andrea L - (algranth) wrote: > Pam, > I can tell you that NSH is totally aware of this situation and one of our goals is to try to do something about it. These things are not done quickly however but little by little we are making some progress. We have partnered with other healthcare professionals like CLSI, NAACLS, CLMA, Health Professions Network, Assoc. of Pathology Chairs just to name a few (for a better list check out the NSH web site). All this in an effort to make histotechnology and histotechs known in the healthcare community. Programs like the Career Days and the Histotechnology Professionals Day were created for this purpose as well. These things don't happen by themselves either, we all have to take the responsibility of getting the word out. I know that you are doing what you can - I am too, speaking at local schools and service clubs. For HPD I had an article in our local newspaper. We just need more techs willing to do these things. > > When techs can't travel to an educational venue there are teleconferences, the online learning center ( especially for your OJT's) and CEU's from the Journal just to name some alternatives. > > We do need more schools but this is difficult. We had a very successful program here at our Community College but (and because of lack of understanding and some political stuff too) they discontinued it last year. This thread is interesting to me for several reasons. I was considering adding a Histology training program here at Delta, and I have run into the same problems as everyone else. First, there is no money here to start new programs. Despite having most of the equipment available and an offer from a local supply house for assistance, our campus is broke and getting broker. No new programs on the horizon, some existing programs are being cut or eliminated. We could probably swing it, except for the instructor costs. Unless someone is willing to teach the classes for free, I don't think we would have the $$ to pay them. Next, we have no place to send students for their internships. Most of the path labs around here are small and we might be able to place, maybe, 3 students a semester. The local folks are very supportive and if 3 students a year would sustain a program here we would go for it. I also lack much in the way of contacts with the local healthcare professionals. Until we found the local vendor, I was lost in getting professionals for the advisory board, evaluation process, and review committees. Our local vendor stepped right up and offered to make contacts with labs and pathologists who could sign on and sign off where needed, but that will still not allow a programs to get started here. Bottom line is that the bean counters now require at least 20 - 25 students per class to cover expenses. Because of the way we are funded, students enrolled are worth so much, it takes at least 20 students to break even. Even if we could enroll 20 students every time the class was offered, where would I send them for internships around here? Our nursing program is in a similar boat. They can fill the classes, they have long waiting lists and had to go to a lottery to select students. But, they are limited by locations for students to get their clinical work. Wishing it were different, Jon Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp@deltacollege.edu From karen <@t> gateslinger.com Mon May 9 11:47:22 2011 From: karen <@t> gateslinger.com (Karen Lahti) Date: Mon May 9 11:47:22 2011 Subject: [Histonet] Biocare Intelipath In-Reply-To: References: Message-ID: <00bd01cc0e68$c513f970$4f3bec50$@com> Sheila, I have been a long time Biocare user, as well as many years of experience with just about every IHC company/instrument on the market today. I have had a fair share of "issues" with each. I am amazed continuously at the attention and care I am given from the whole Biocare company. When I call with even a minor maybe problem, I do feel as if I am their only customer. I get immediate response. The IntelliPath instrument does require more tech time to prepare the slides which I resisted from the very beginning. I have learned the flexibility this has brought to validation and overall quality of staining. The antibodies and detection are exceptional in comparison to other vendors. The detection coupled with the instrument enabled our lab to drastically reduce the cost of each stain and keep a high level of quality that is reproducible from day to day. Karen Karen D. Lahti, HT(ASCP)QIHC, MLT(AMT) Histology Supervisor Arizona Digestive Health 1300 N. 12th Street #550 Phoenix, AZ 85006 480-236-6559 cell 602-687-7218 office -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Sunday, May 08, 2011 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare Intelipath Hello Netters We are considering an Intelipath. I'd like some opinions on the instrument from users out there. Thanks in advance!!! Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From araniqkslvr <@t> yahoo.com Mon May 9 13:34:03 2011 From: araniqkslvr <@t> yahoo.com (Paula) Date: Mon May 9 13:34:07 2011 Subject: [Histonet] Re: Anybody Hiring Techs Without Certification? Message-ID: <275029.90805.qm@web30302.mail.mud.yahoo.com> >From the way some labs act, it seems like they don't really?need anyone. The last time I worked in the field was late 80s. I am willing to buy books and refresh my knowledge. Schools say I don't need a degree again, to brush up my skills in a lab. The labs say I haven't done it recently and therefore no job. One place was willing to train a person with a B.S. in science, but since my B.S. was not in science, I wasn't eligible. I am certified. People act like I cannot re-learn it. ? Paula From relia1 <@t> earthlink.net Mon May 9 14:06:57 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon May 9 14:06:53 2011 Subject: [Histonet] Re: Anybody Hiring Techs Without Certification? Message-ID: <1577A33DE4924024BDC7BA9F121C6EB2@ownerf1abaad51> Hi Everybody, I have been watching this thread and would like to add my "two cents" What I have observed is that there are a lot of labs out there that have openings but no urgency to fill them unless they find "the ideal candidates" ideal meaning ASCP HT/HTL with 5 years of experience willing to relocate at their own expense. This is how it is in most professions right now. The urgency was there a few years ago before the economy slowed down. Back then labs needed people "yesterday" so they were more willing to invest in a histotech returning to the field, an uncertified tech or an entry level tech. Nowadays, since the volume has gone down in many labs they have no sense of urgency to incentify them to be more flexible in their hiring considerations. This is an observation based on conversations with employers and histotechs. It is not just a matter of only wanting to pay a fee for "suitable candidates" I have given away leads to histotechs to no avail. If you want to affect change beyond the NSH and the ASCP I urge managers to share "success" stories with other managers specifically about being flexible and hiring the qualified candidates as opposed to the "ideal" candidate. Just a thought. -----Original Message----- From: "histonet-bounces@lists.utsouthwestern.edu" Sent: 5/9/2011 2:35 PM To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Re: Anybody Hiring Techs Without Certification? >From the way some labs act, it seems like they don't really need anyone. The last time I worked in the field was late 80s. I am willing to buy books and refresh my knowledge. Schools say I don't need a degree again, to brush up my skills in a lab. The labs say I haven't done it recently and therefore no job. One place was willing to train a person with a B.S. in science, but since my B.S. was not in science, I wasn't eligible. I am certified. People act like I cannot re-learn it. Paula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisls.com Mon May 9 14:14:08 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Mon May 9 14:14:36 2011 Subject: [Histonet] PA HT Postition Message-ID: <6F33D8418806044682A391273399860F080E2A08@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! We are an 8 physician Dermatology practice located in Bucks County, PA looking for a certified HT or HTL. Candidate must meet CLIA grossing requirements. The candidate will be responsible for routine histology duties. This is a part-time position that offers a competitive salary and flexible hours. Interested applicants should contact Meredith Hale, phone (214)596-2219 or e-mail mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 214-596-7095 mhale@carisls.com From rjr6 <@t> psu.edu Mon May 9 14:18:33 2011 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Mon May 9 14:18:58 2011 Subject: [Histonet] Procedure Manual Template In-Reply-To: <718642.41680.qm@web84302.mail.re1.yahoo.com> References: <718642.41680.qm@web84302.mail.re1.yahoo.com> Message-ID: Did you try NSH? I got one from them years ago to write up my SOP's. Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SHANE NELSON Sent: Saturday, May 07, 2011 11:00 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Procedure Manual Template Heather I feel your pain, Please contact me on my personal e-mail. I can try to help. I had to write my own procedure manuals also. THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Mon May 9 17:10:32 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon May 9 17:10:36 2011 Subject: [Histonet] Re: Is anybody hiring techs without a certification? Message-ID: I'm certainly glad that Yvette and I initiated such a spirited discussion of the discouraging issue of certification in histotechnology! Pathologists must bear considerable blame here, for not taking a much stronger stand in defense of the people who make the practice of pathology possible. There are multiple issues, like the poor background most pathologists have in histotechnology, but I think the biggest problem has been the timidity of pathologists in addressing the issue. Since about 1980 the job market for pathologists in the USA has fluctuated between bad and worse, and the result has been that a group of physicians already self-selected for timidity has studied and practiced in a culture of timidity. Pathologists are extraordinarily reluctant to advocate anything that might endanger their employment. Pathologists haven't stood up for themselves, let alone for anyone else. Pathologists' organizations, in particular the College of American Pathologists, have not been very effective in addressing this issue. Bob Richmond Samurai Pathologist Knoxville TN From techmana12 <@t> yahoo.com Mon May 9 19:50:33 2011 From: techmana12 <@t> yahoo.com (Dorothy Glass) Date: Mon May 9 19:50:37 2011 Subject: [Histonet] (no subject) Message-ID: <478757.23396.qm@web114504.mail.gq1.yahoo.com> What is an IHC stain for gastric reflux. Pathologists are wanting to start using the procedure. Can anyone help? From hrumbut <@t> yahoo.com Mon May 9 21:38:56 2011 From: hrumbut <@t> yahoo.com (Heather R) Date: Mon May 9 21:39:00 2011 Subject: [Histonet] Old VIP cassette baskets Message-ID: <278518.4015.qm@web130205.mail.mud.yahoo.com> Does anyone know where I can find a basket that was used in the older VIP processors? Its about 4.5 x 5 inches, holds maybe 50 cassettes laying down on their side.? I'm not familiar with where to find used Histology equipment. If someone has one for sale I would love to hear from you. Thanks Heather From kmerriam2003 <@t> yahoo.com Tue May 10 08:12:15 2011 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue May 10 08:12:20 2011 Subject: [Histonet] Biocare Intelipath In-Reply-To: <00bd01cc0e68$c513f970$4f3bec50$@com> References: <00bd01cc0e68$c513f970$4f3bec50$@com> Message-ID: <984472.39441.qm@web130107.mail.mud.yahoo.com> I second Karen's comments about Biocare and the Intellipath.? I work in an early discovery research pathology lab at a large biotech firm.? We do not have a standard set of stains that we run (we are testing new ones all the time), so the open-ness of the instrument was key.? You can use any antibody or detection system (we use antibodies from many sources, including those made in-house), but we tend to use Biocare's detection reagents for chromogenic staining.? We also do all of our IF staining on the machine. I am a long-time customer of Biocare's and I even got to beta-test the Intellipath instrument a few years ago.? They are by far one of the best companies I have ever worked with, they are super customer-oriented and the service engineers come quickly, if your machine needs to be fixed. I would defintely get a demo into your lab to see if it fits your needs. Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: Karen Lahti To: Sheila Adey ; histonet@lists.utsouthwestern.edu Sent: Mon, May 9, 2011 12:47:22 PM Subject: RE: [Histonet] Biocare Intelipath Sheila, I have been a long time Biocare user, as well as many years of experience with just about every IHC company/instrument on the market today.? I have had a fair share of "issues" with each.? I? am amazed continuously at the attention and care I am given from the whole Biocare company. When I call with even a minor maybe problem, I do feel as if I am their only customer. I get immediate response.? The IntelliPath instrument does require more tech time to prepare the slides which I resisted from the very beginning. I have learned the flexibility this has brought to validation and overall quality of staining.? The antibodies and detection are exceptional in comparison to other vendors.? The detection coupled with the instrument enabled our lab to drastically reduce the cost of each stain and keep a high level of quality that is reproducible from day to day.? Karen Karen D. Lahti, HT(ASCP)QIHC, MLT(AMT) Histology Supervisor Arizona Digestive Health 1300 N. 12th Street #550 Phoenix, AZ? 85006 480-236-6559 cell 602-687-7218 office ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Sunday, May 08, 2011 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare Intelipath Hello Netters We are considering an Intelipath. I'd like some opinions on the instrument from users out there. Thanks in advance!!! Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue May 10 08:35:08 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue May 10 08:35:16 2011 Subject: [Histonet] Biocare Intelipath In-Reply-To: <984472.39441.qm@web130107.mail.mud.yahoo.com> References: <00bd01cc0e68$c513f970$4f3bec50$@com> <984472.39441.qm@web130107.mail.mud.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBD77D981@IBMB7Exchange.digestivespecialists.com> I'd have to third the comments about Biocare and the Intellipath. Biocare has to be the best company I have dealt with in all of the years I have been in this field in the area of support and customer service. One phone call with even a "maybe" issue gets the whole team involved. That reason alone makes the Intellipath a great instrument. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Tuesday, May 10, 2011 9:12 AM To: Karen Lahti; Sheila Adey; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Biocare Intelipath I second Karen's comments about Biocare and the Intellipath.? I work in an early discovery research pathology lab at a large biotech firm.? We do not have a standard set of stains that we run (we are testing new ones all the time), so the open-ness of the instrument was key.? You can use any antibody or detection system (we use antibodies from many sources, including those made in-house), but we tend to use Biocare's detection reagents for chromogenic staining.? We also do all of our IF staining on the machine. I am a long-time customer of Biocare's and I even got to beta-test the Intellipath instrument a few years ago.? They are by far one of the best companies I have ever worked with, they are super customer-oriented and the service engineers come quickly, if your machine needs to be fixed. I would defintely get a demo into your lab to see if it fits your needs. Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: Karen Lahti To: Sheila Adey ; histonet@lists.utsouthwestern.edu Sent: Mon, May 9, 2011 12:47:22 PM Subject: RE: [Histonet] Biocare Intelipath Sheila, I have been a long time Biocare user, as well as many years of experience with just about every IHC company/instrument on the market today.? I have had a fair share of "issues" with each.? I? am amazed continuously at the attention and care I am given from the whole Biocare company. When I call with even a minor maybe problem, I do feel as if I am their only customer. I get immediate response.? The IntelliPath instrument does require more tech time to prepare the slides which I resisted from the very beginning. I have learned the flexibility this has brought to validation and overall quality of staining.? The antibodies and detection are exceptional in comparison to other vendors.? The detection coupled with the instrument enabled our lab to drastically reduce the cost of each stain and keep a high level of quality that is reproducible from day to day.? Karen Karen D. Lahti, HT(ASCP)QIHC, MLT(AMT) Histology Supervisor Arizona Digestive Health 1300 N. 12th Street #550 Phoenix, AZ? 85006 480-236-6559 cell 602-687-7218 office ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Sunday, May 08, 2011 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare Intelipath Hello Netters We are considering an Intelipath. I'd like some opinions on the instrument from users out there. Thanks in advance!!! Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue May 10 08:47:50 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 10 08:47:54 2011 Subject: [Histonet] (no subject) In-Reply-To: <478757.23396.qm@web114504.mail.gq1.yahoo.com> References: <478757.23396.qm@web114504.mail.gq1.yahoo.com> Message-ID: <837362.55689.qm@web65714.mail.ac4.yahoo.com> The so called "reflux" is a symptom of a gastric condition and, as such, cannot be detected by HC or IHC. Perhaps a gastric biopsy could reflect an anatomical alteration that accompanies it but cannot measure. Have your pathologists seriously consider this issue? Ren? J. From: Dorothy Glass To: Histonet@lists.utsouthwestern.edu Sent: Monday, May 9, 2011 8:50 PM Subject: Re: [Histonet] (no subject) What is an IHC stain for gastric reflux. Pathologists are wanting to start using the procedure. Can anyone help? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Tue May 10 09:15:45 2011 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue May 10 09:38:08 2011 Subject: [Histonet] RE: Histonet Digest: Is anybody hiring techs without a certification? In-Reply-To: References: Message-ID: Yvette, To answer your original question, Yes, non certified techs can be hired. However, the tide is turning in that respect. Non certified techs, in some places where registry is required, may be classified as aides or assistants, receiving the salary for that position. In some labs the noncertified tech is the most experienced and knowledgable, but the lowest paid. Keep that in mind. Now to the discussion on programs for histotechnology. I currently work as an instructor for a local program and face the same issues as the others, low exposure, limited clinical sites, no funds, and a need to increase enrollment yesterday. Our program is the oldest at our facility (1949ish), and yet very few places know of our existence. I have gone to local colleges to recruit students, but the professors there don't even know about us. Most four year institutions are afraid that we will steal their students, since we offer the HTL bachelor's, but I have appealed to the Pre-Med, Pre-Pharm, and Pre-Nursing students who have the prereqs, and didn't get into the school of choice. We will see if this appeal works. I fell into histology that way and have never regretted it. Others have as well. It did get me in the door, and that is saying something, since our school recruiter couldn't do that. Here in Houston, some labs are asking for HTL's for mangement tracks and IHC operations. So we are hopeful in that aspect. Actually we have a local CC offering an AAS HT program as well, and are getting some inquiry's about continuing on in our program for a bachelor's. We see the need for qualified staff, but in trying to fill it, we wonder if we meet our goal of minimum students will we flood the market locally? A good portion of our students are mature. How will we provide quality training and instruction in such a large quantity? Will the local and national chapters of the organization support our endeavors by getting the word out to the employers about certifed staff? And finally, will the pathologists and employers give us the respect we deserve with adequate pay? Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Yvette asks: >>Is anybody hiring techs without a certification?<< Is anybody hiring techs WITH a certification? With a coming retirement, the two labs I'm working in now will have NO certified histotechs (total of four workers) - all OJT's. Some of them probably have the educational qualifications to sit the exam, but management isn't very sympathetic - I'll have to buy them a copy of the 3rd edition of Freida Carson's book, for starters. Bob Richmond Samurai Pathologist Knoxville TN Currently we have too few schools and not enough slots in the schools we have for Histology to grow ans educate people properly.? It is hard to tell if the ASCP and NSH are just unaware of the issues or not interested as we see schools close and career days at NSH for high school students yet no real action.???They need to assist?what many of us are doing now - OJT with people who have the AA or AS degree and hope they stay in the field.? The majority of people I meet and tell what I do have no idea that the field of Histology exists or how important it can be to a patient having surgery and awaiting a diagnosis.? I currently have two people we are training as we?can't find qualified people here and at least they are the best we could find.? The second issue is pay and the fact that any?Histology area controlled by Clinical Laboratory is in an uphill battle to get?salaries raised as the CP people all seem to feel if you?don't have a BS you can't rate better pay.? I do realize we have many? MTs in Histology and they usually great?for us.? It is the management that cannot get by the requirements to help us go forward.? They should look at the fact many of the Histologist now working will retire in the next ten years and then where will they be in finding people for this field? Pam Marcum Personal Option The NSH and ASCP are well aware of the lack of schools, but are powerless to do anything about it. With cutbacks in education and healthcare schools and hospitals are cutting back on training as it takes time and money. What type of assistance are you speaking about for the OJT people who have the AA/AS? The ASCP recognizes these individuals through route 2 for the HT (ASCP) exam. One of the reasons that our program and others do not take more students is that we cannot find facilities that are willing to take on the clinical experience for students. Many of the histology labs are unwilling or unable to train students. What type of action would you like the NSH and ASCP to take? Jennifer MacDonald Jennifer, I am not sure you understood that I know the paths to getting registration.? We can argue this forever however;?the outcome is the same too little; too late for Histology to grow.? No one in?a Med Tech program since the middle 60's?is required to even set foot in Histology so we are the not known and will not be until we or one of these organizations goes beyond lip?service and promises.??After 40 years I am still asked what kind of history I teach.? I explain what we do and generally hear of that's great, never knew you guys existed.? I go to schools when I can someone to invite me and still it is not a field anyone knows. ??We currently have two people training in our lab.? One has an AA and the other an AS degree.? Both are taking additional science and chemistry courses to help them understand?what they are being taught.? I am the person in charge of the Histology Lab, the Gross Room and the Morgue.? So, Yes I know the paths for all three areas.? Unfortunately, the ASCP and NSH are aware and still when there is a meeting for CLMA , ASCP or other areas of the laboratory you will be lucky to find a lecture on anything to do with Histology whether local or national.? No funds are available for anyone to go to a meeting or travel.??IF NSH is not local most people can't get there.? So exactly where are we to get education CEUs if no one will pay for them and we are at the lowest end of the pay scale and can't?? Most of the?Histologist here are unable to travel more than 10 to 50 miles for a meeting even in the state.?? We are at a University and have been told point blank no education money (time off only), no classes unless they are free and oh by the way be sure the new ones get their CEUs to keep their registries.? We have also been told anyone without a BS will be paid like a LST and the HT does not qualify them for a raise or higher pay.? I have friends at other major institutes who are being told the same thing.? We ask in the early 90's for the education levels to be raised by NSH and still took years to happen.? I have been in Histology for over 40 years and still love the field, just hate the way we are treated in comparison to the CP area.? Try hiring someone under the new guidelines for grossing without a PA license and see what you get.? It is just as hard and just a few schools for that field too.?? When you get the PA license try getting the pay outside a major metropolitan area. Pam Marcum Still my opinion " Pamela, Thank you for clarifying. This is not what I understood from you first post. Jennifer *********************************** From PAMarcum <@t> uams.edu Tue May 10 09:49:46 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue May 10 09:49:51 2011 Subject: [Histonet] Re: Is anybody hiring techs without a certification? In-Reply-To: References: Message-ID: I thanked Bob offline last night for his comments on pathologists temerity on our field. I have worked with and met some who are anything but timid however, most are not aggressive in political issues at institutions which is what this falls under. We as histologists have not always helped our own cause so I think we may be even on that count. I hope this discussions helps those in our field and organizations we belong to as members to understand we are interested and we are looking a very severe crisis for the future of Histology. Blame will not help only action! I am sorry if I offended anyone however; we have heard for years "HELP IS COMING" and not seen it. I just get angry and say my peace from time to time. I love this field and hate to see us lower the standards back to where I started in 1965 with research before that. The field was complex in some ways just not like it is now and we need more education to be really good at the work that is so important to the patient who's tissue is on the slide. We need to understand special stains at a chemical reaction level. IHC at many smaller facilities is not a separate department so the histologist must have a more than working knowledge of it to troubleshoot problems and add new antibodies. Instrumentation cannot do it all so, a good basic understanding of IHC is a requirement. Then we have the new instrumentation for everything from grossing to the slides we use now and why as well as how it has changed. Someone in an OJT position may never have the chance to learn it all and be their best for the future if they are only taught how to take the test and pass, not why they do it. In most facilities we only have time for a part of the training actually needed to help add a new person to our field. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, May 09, 2011 5:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Is anybody hiring techs without a certification? I'm certainly glad that Yvette and I initiated such a spirited discussion of the discouraging issue of certification in histotechnology! Pathologists must bear considerable blame here, for not taking a much stronger stand in defense of the people who make the practice of pathology possible. There are multiple issues, like the poor background most pathologists have in histotechnology, but I think the biggest problem has been the timidity of pathologists in addressing the issue. Since about 1980 the job market for pathologists in the USA has fluctuated between bad and worse, and the result has been that a group of physicians already self-selected for timidity has studied and practiced in a culture of timidity. Pathologists are extraordinarily reluctant to advocate anything that might endanger their employment. Pathologists haven't stood up for themselves, let alone for anyone else. Pathologists' organizations, in particular the College of American Pathologists, have not been very effective in addressing this issue. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From jennifer.harvey <@t> Vanderbilt.Edu Tue May 10 10:19:15 2011 From: jennifer.harvey <@t> Vanderbilt.Edu (Harvey, Jennifer Lynn) Date: Tue May 10 10:19:22 2011 Subject: [Histonet] Prisma Stainer Containers Message-ID: I just got a new Sakura Prisma Stainer and it came with the large staining containers and really need the smaller ones (sakura #6145) Does anyone have any of the small staining reservoir that they might like to trade for the large ones? Jennifer Harvey, HT(ASCP) QIHC Vanderbilt University Medical Center Neuropathology Lab Supervisor C-2309 Medical Center North Nashville, TN 37232-2561 Phone: 615-343-0083 Fax: 615-343-7089 From techonebs <@t> comcast.net Tue May 10 11:01:54 2011 From: techonebs <@t> comcast.net (Matt Mincer) Date: Tue May 10 11:01:02 2011 Subject: [Histonet] re Old VIP cassette baskets Message-ID: <4DC96172.2060008@comcast.net> Hi Heather, We sell the old style Sakura baskets. We have the 50 and 100 cassette models. We also sell a 150 cassette deluxe model. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 From mpowers <@t> dpspa.com Tue May 10 11:06:27 2011 From: mpowers <@t> dpspa.com (Marian Powers) Date: Tue May 10 11:06:31 2011 Subject: [Histonet] CAP 15189 Message-ID: Hi all, Anyone out there who are CAP 15189 accredited and would share your experiences with me on the process? Thanks in advance, Marian -- *Marian L. Powers* Manager, Technical Operations *Doctors Pathology Services * ** c| 302.747.0580 o| 302.677.0000 ext: 110 f | 302.677.0010 From mward <@t> wfubmc.edu Tue May 10 11:27:50 2011 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue May 10 11:28:04 2011 Subject: [Histonet] Thanks for the hair processing information Message-ID: Thanks so much to the folks that responded to my request for information. I forwarded them on to my colleague. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 From sbreeden <@t> nmda.nmsu.edu Tue May 10 13:21:44 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue May 10 13:21:48 2011 Subject: [Histonet] 10% NBF "Shelf Life"? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4786C@nmdamailsvr.nmda.ad.nmsu.edu> I have just come from the Histonet Archives (cough, cough...) and my Extensive Library of Histology Reference Books (Sheehan, Carson and Lillie) and have found nothing on the supposed or generally-accepted "shelf-life" of 10% NBF. Is there any such number? For example, we make our own 10% NBF in 20L carboys; our usage varies from day to day so sometimes it's used quickly and sometimes it sits for several weeks. It has been proposed to me that we add "shelf life" date to our carboys when a new batch is made. I know there is someone out there that has investigated this before and can give me a time and a reference (and I need the reference as well for completeness). Thank you so much - I'm standing by. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From brinkerk <@t> musc.edu Tue May 10 13:28:56 2011 From: brinkerk <@t> musc.edu (Geils, Karen Brinker) Date: Tue May 10 13:29:01 2011 Subject: [Histonet] HTL Certification - Distance Education at MUSC Message-ID: The Medical University of South Carolina offers a distance education option for practicing histotechnicians who wish to sit for the HTL certification exam. The program is designed so that current employees in a histotechnology laboratory can maintain their employment and gain the educational requirements needed for the ASCP's BOR certification eligibility. All didactic and program material is delivered via the web and clinical experience is gained in the techs laboratory. The program is one year in length commencing in September and April of each year. Our website has more information about the program. Karen Brinker Geils, MS, HT(ASCP), CT(ASCP) Director, Histotechnology Program Department of Pathology and Laboratory Medicine Medical University of South Carolina 843-792-4013 From Timothy.Morken <@t> ucsfmedctr.org Tue May 10 14:55:15 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue May 10 14:55:29 2011 Subject: [Histonet] Formalin filtering, reuse, expiration date questions Message-ID: For those using formalin filtering to recycle formalin (i.e. Creative Waste Solutions formalin filtering unit or other methods), how have you liked it? How many times do you reuse the formalin (and how do you tell?!) How do you handle an expiration date for the filtered formalin? For those using the Creative Waste unit, how well does the assay work for determining concentration? Thanks for any and all info!! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center From Katrina.Scrivens <@t> lhsc.on.ca Tue May 10 14:56:51 2011 From: Katrina.Scrivens <@t> lhsc.on.ca (Katrina Scrivens) Date: Tue May 10 14:57:06 2011 Subject: [Histonet] Neuropathology quiz Message-ID: <4DC96043.FD5A.0011.1@lhsc.on.ca> Hi there, I am wondering if anyone has some quiz questions about the Neuropathology lab? I am looking for questions about lab procedures, staining, etc. Katrina LHSC London, Ontario -------------------------------------------------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Tue May 10 15:56:03 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 10 15:56:08 2011 Subject: [Histonet] Formalin filtering, reuse, expiration date questions In-Reply-To: References: Message-ID: <560700.72643.qm@web65716.mail.ac4.yahoo.com> This is more a concern than an answer: if you filter used formalin to eliminate any tissue remnants/contaminants left from the previous usage, you will be dealing with a formalin solution of LESS than 4% because some of the formaldehyde (methylene-glycol) has been retained in the tissues. Unless you reconstitute the formalin (adjusting the concentration to 4%) theoretically you will end with a close to 0% formalin. How do you deal with this issue? Ren? J. From: "Morken, Timothy" To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, May 10, 2011 3:55 PM Subject: Re: [Histonet] Formalin filtering, reuse, expiration date questions For those using formalin filtering to recycle formalin (i.e. Creative Waste Solutions formalin filtering unit or other methods), how have you liked it? How many times do you reuse the formalin (and how do you tell?!) How do you handle an expiration date for the filtered formalin? For those using the Creative Waste unit, how well does the assay work for determining concentration? Thanks for any and all info!! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Tue May 10 18:06:27 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue May 10 18:06:35 2011 Subject: [Histonet] Yvette, here is your answer Message-ID: Yvette, I too graduated from AFIP. I waited several years between getting my training and sitting for the HTL exam, earning my B.S. in the interim. During this time I worked in the clinical and research lab, and even started my travel career (without being registered). Every one of these positions was advertised as requiring a registry. "How can this be?", you ask? Whenever the employer would ask me if I was registered, I would reply, "No, but I was trained at AFIP." In *every*case, the registry requirement was waived. You have been the beneficiary of some of the finest technical training available anywhere. It would be a crime not to use it. Thank you for your service, Jay A. Lundgren, M.S., HTL (ASCP) From wdesalvo.cac <@t> hotmail.com Tue May 10 22:08:54 2011 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue May 10 22:08:58 2011 Subject: [Histonet] Formalin filtering, reuse, expiration date questions In-Reply-To: References: Message-ID: I have used the Creative Waste Systems, Alcohol and Formalin for 4+ yrs. and like them very much. The process is simple, the filtered solution is quickly tested and used. There is no distillation of the solution, only removal of elements by the slurry to clean the reagent. We reuse alcohol and formalin for >6 months, adding new solution to adjust concentration. You use a hydrometer for the alcohol solution and there is a pH and formaldehyde concentration test for the 10% NBF. You will perform a validation study comparing the filtered/tested to new solution. Keep the documentation to show your licensing agencies. Re-validate if you change the process. You will follow CAP rules for validating the use of the solution in your process. Label the secondary container that contains tested and filtered solution, according to your validation process. You will continue to test and label as you fill you secondary container. To determine the concentration, you simply use a hydrometer and graduated cylinder. William DeSalvo, B.S., HTL(ASCP) > From: Timothy.Morken@ucsfmedctr.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 10 May 2011 12:55:15 -0700 > Subject: [Histonet] Formalin filtering, reuse, expiration date questions > > > For those using formalin filtering to recycle formalin (i.e. Creative Waste Solutions formalin filtering unit or other methods), how have you liked it? > > How many times do you reuse the formalin (and how do you tell?!) > > How do you handle an expiration date for the filtered formalin? > > For those using the Creative Waste unit, how well does the assay work for determining concentration? > > Thanks for any and all info!! > > Tim Morken > Supervisor, Histology, IPOX > UC San Francisco Medical Center > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From member <@t> linkedin.com Tue May 10 22:33:37 2011 From: member <@t> linkedin.com (Wanda jones via LinkedIn) Date: Tue May 10 22:33:40 2011 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <1954101059.820031.1305084817425.JavaMail.app@ela4-bed84.prod> LinkedIn ------------Wanda jones requested to add you as a connection on LinkedIn: ------------------------------------------ Jackie, I'd like to add you to my professional network on LinkedIn. - Wanda Accept invitation from Wanda jones http://www.linkedin.com/e/yvpgd1-gnjpsxxs-j/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1324412348_3/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPnPwQcP8Nd3gOcP59bSZFck9bgSZ4bPgTcPoUd3gRe38LrCBxbOYWrSlI/EML_comm_afe/ View invitation from Wanda jones http://www.linkedin.com/e/yvpgd1-gnjpsxxs-j/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1324412348_3/3dve3gPcz4Qd38PckALqnpPbOYWrSlI/svi/ ------------------------------------------ DID YOU KNOW you can showcase your professional knowledge on LinkedIn to receive job/consulting offers and enhance your professional reputation? Posting replies to questions on LinkedIn Answers puts you in front of the world's professional community. http://www.linkedin.com/e/yvpgd1-gnjpsxxs-j/abq/inv-24/ -- (c) 2011, LinkedIn Corporation From member <@t> linkedin.com Tue May 10 22:38:08 2011 From: member <@t> linkedin.com (Wanda jones via LinkedIn) Date: Tue May 10 22:38:11 2011 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <1148422942.868688.1305085088061.JavaMail.app@ela4-bed79.prod> LinkedIn ------------Wanda jones requested to add you as a connection on LinkedIn: ------------------------------------------ Jackie, I'd like to add you to my professional network on LinkedIn. - Wanda Accept invitation from Wanda jones http://www.linkedin.com/e/yvpgd1-gnjpyqt6-9/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1324419688_3/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPnPwUdzANd3gOcP59bSZFck9bgSZ4bPgTcPoUd3gRe38LrCBxbOYWrSlI/EML_comm_afe/ View invitation from Wanda jones http://www.linkedin.com/e/yvpgd1-gnjpyqt6-9/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1324419688_3/3dve3wSej4Qd38PckALqnpPbOYWrSlI/svi/ ------------------------------------------ DID YOU KNOW you can showcase your professional knowledge on LinkedIn to receive job/consulting offers and enhance your professional reputation? Posting replies to questions on LinkedIn Answers puts you in front of the world's professional community. http://www.linkedin.com/e/yvpgd1-gnjpyqt6-9/abq/inv-24/ -- (c) 2011, LinkedIn Corporation From louise.renton <@t> gmail.com Wed May 11 01:35:17 2011 From: louise.renton <@t> gmail.com (Louise Renton) Date: Wed May 11 01:35:21 2011 Subject: [Histonet] (no subject) In-Reply-To: <837362.55689.qm@web65714.mail.ac4.yahoo.com> References: <478757.23396.qm@web114504.mail.gq1.yahoo.com> <837362.55689.qm@web65714.mail.ac4.yahoo.com> Message-ID: could they possibly mean a H pylori? associated with gastric ulcers On Tue, May 10, 2011 at 3:47 PM, Rene J Buesa wrote: > The so called "reflux" is a symptom of a gastric condition and, as such, > cannot be detected by HC or IHC. Perhaps a gastric biopsy could reflect an > anatomical alteration that accompanies it but cannot measure. Have your > pathologists seriously consider this issue? > Ren? J. > > From: Dorothy Glass > To: Histonet@lists.utsouthwestern.edu > Sent: Monday, May 9, 2011 8:50 PM > Subject: Re: [Histonet] (no subject) > > What is an IHC stain for gastric reflux. Pathologists are wanting to start > using > the procedure. Can anyone help? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From BMolinari <@t> heart.thi.tmc.edu Wed May 11 06:41:59 2011 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Wed May 11 06:42:03 2011 Subject: [Histonet] stainless steel cassettes Message-ID: Hi , Does anyone have any stainless steel cassettes they would like to sell or does anyone know where we could purchase them? As always, thank you for your help and happy hump day! Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) From kim.tournear <@t> yahoo.com Wed May 11 09:06:33 2011 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Wed May 11 09:06:37 2011 Subject: [Histonet] Peloris protocols Message-ID: <603760.86759.qm@web120218.mail.ne1.yahoo.com> Hi everyone, I am looking for anyone who is using protocols for the xylene free or xylene substitues on the Peloris tissue processor in the Florida area. Would anyone be willing to share them with me, as well as any validation protcols for Her2? You can reply back at this address. Thanks in advance for your help. Kim Tournear -?HT, QIHC (ASCP) Field Support Specialist Core Histology - Southeast Leica Microsystems Inc. ? ~Don't be afraid your life will end, be afraid it will never begin~ From amber.mckenzie <@t> gastrodocs.net Wed May 11 10:01:03 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed May 11 10:00:59 2011 Subject: [Histonet] IHC run print outs In-Reply-To: <986954.21674.qm@web81002.mail.mud.yahoo.com> References: <201104291700.p3TH0nw3030579@nlpi138.prodigy.net> <986954.21674.qm@web81002.mail.mud.yahoo.com> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> Does everyone print out IHC run reports after each run? If so, how long do you keep them and are the pathologists supposed to sign off on them with their slides? From LSebree <@t> uwhealth.org Wed May 11 10:08:07 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed May 11 10:08:11 2011 Subject: [Histonet] IHC run print outs In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> References: <201104291700.p3TH0nw3030579@nlpi138.prodigy.net><986954.21674.qm@web81002.mail.mud.yahoo.com> <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> Message-ID: We don't do that currently Amber, but I think its something we will look at doing in the future. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, May 11, 2011 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC run print outs Does everyone print out IHC run reports after each run? If so, how long do you keep them and are the pathologists supposed to sign off on them with their slides? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Wed May 11 10:09:09 2011 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed May 11 10:09:13 2011 Subject: [Histonet] IHC run print outs In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> References: <201104291700.p3TH0nw3030579@nlpi138.prodigy.net>, <986954.21674.qm@web81002.mail.mud.yahoo.com>, <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> Message-ID: To meet CAP requirements, we print un reports and send send to the pathologists with slides, they review, sign off and we keep for 2 yrs. All QC paperwork is kept for 2 yrs. William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 11 May 2011 10:01:03 -0500 > From: amber.mckenzie@gastrodocs.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC run print outs > > > > Does everyone print out IHC run reports after each run? If so, how long > do you keep them and are the pathologists supposed to sign off on them > with their slides? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed May 11 10:12:04 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed May 11 10:12:09 2011 Subject: [Histonet] IHC run print outs In-Reply-To: References: <201104291700.p3TH0nw3030579@nlpi138.prodigy.net>, <986954.21674.qm@web81002.mail.mud.yahoo.com>, <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081F5A0AC6@CHEXCMS10.one.ads.che.org> Same here Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, May 11, 2011 11:09 To: amber.mckenzie@gastrodocs.net; histonet Subject: RE: [Histonet] IHC run print outs To meet CAP requirements, we print un reports and send send to the pathologists with slides, they review, sign off and we keep for 2 yrs. All QC paperwork is kept for 2 yrs. William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 11 May 2011 10:01:03 -0500 > From: amber.mckenzie@gastrodocs.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC run print outs > > > > Does everyone print out IHC run reports after each run? If so, how > long do you keep them and are the pathologists supposed to sign off on > them with their slides? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rjbuesa <@t> yahoo.com Wed May 11 10:15:02 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 11 10:15:10 2011 Subject: [Histonet] IHC run print outs In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> References: <201104291700.p3TH0nw3030579@nlpi138.prodigy.net> <986954.21674.qm@web81002.mail.mud.yahoo.com> <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> Message-ID: <177114.46366.qm@web65709.mail.ac4.yahoo.com> Only when I received out automaton from DAKO to make sure that everything was done correctly. I stopped doing that after all the protocols were validated. It was an extra step that just added more time consuming unnecessary documentation. Ren? J. From: Amber McKenzie To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 11, 2011 11:01 AM Subject: [Histonet] IHC run print outs Does everyone print out IHC run reports after each run? If so, how long do you keep them and are the pathologists supposed to sign off on them with their slides? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Wed May 11 10:16:11 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed May 11 10:16:15 2011 Subject: [Histonet] Re: Her2 CAP survey In-Reply-To: <4DC2CAEB.7400.0077.1@harthosp.org> References: <4DC2CAEB.7400.0077.1@harthosp.org> Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AC43@EXCHANGE3.huntingtonhospital.com> Our staining was also light. I called CAP and they said to leave the result area blank, mark the bubble for "unable to analyze (11)", and to write an explanation on the last page. My pathologist is questioning why we wouldn't mark "specimen unsatisfactory (33)." What are others that had light staining doing and/or what have you been told by CAP? CAP also told me that they didn't have any replacement slides to send out. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, May 05, 2011 1:06 PM To: Jayne Halli; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Her2 CAP survey Everyone who experiencing problems with the CAP HER2 survey slides needs to contact the CAP to document the problem and request replacement slides. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Jayne Halli" 5/5/2011 3:01 PM >>> Cap validation Her2 slides we got one slide stained great, the other one did not, controls and cases all stained good. We use the Dako system. Jayne Halli HT,ASCP,QIHC Avera McKennan Histology 800 East 21st street Sioux Falls, SD 57117 (605)322-7142 my new e-mail address is jayne.halli@avera.org ----------------------------------------- Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed May 11 10:22:55 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed May 11 10:23:00 2011 Subject: [Histonet] RE: IHC run print outs In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> References: <201104291700.p3TH0nw3030579@nlpi138.prodigy.net> <986954.21674.qm@web81002.mail.mud.yahoo.com>, <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> Message-ID: We currently do. And we have the Dako system. So everytime we print the IHC reports it takes pages and pages. The pathologist are supposed to sign off on them and return them to me. I am then supposed to file them. But....... The major problems are it takes up hundreds of sheets of paper each week. they do not sign them do not return them or return a few months worth of reports all at once we don't have time to file them so that we can retrieve them if needed It was done this way when I got here and I have been trying to figure out a way to stop it. I am not sure why we need them. If the stains are mentioned in the report then we shouldn't need them right? The one thing that is stopping us is that after staining when we organize the slides to hand out, we look at the IHC report to see what pathologist to give them to and how many slides there needs to be. Which is handy when you have over a hundred slides to hand out daily I have asked Dako to add a simplier IHC report to the software. A one page per case report maybe, with the pathologist name and the number of slides stained. This would cut down on the paperwork and make it a lot easier to file and retrieve. Maybe in the next software upgrade.......I am hoping If anyone has any ideas please let me know. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie [amber.mckenzie@gastrodocs.net] Sent: Wednesday, May 11, 2011 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC run print outs Does everyone print out IHC run reports after each run? If so, how long do you keep them and are the pathologists supposed to sign off on them with their slides? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Wed May 11 10:23:09 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Wed May 11 10:26:56 2011 Subject: [Histonet] Re: Her2 CAP survey In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2AC43@EXCHANGE3.huntingtonhospital.com> References: <4DC2CAEB.7400.0077.1@harthosp.org> <57BE698966D5C54EAE8612E8941D76830AD2AC43@EXCHANGE3.huntingtonhospital.com> Message-ID: Laurie, when we called CAP they also told us to use Code 11. That's a GOOD point - specimen unsatisfactory is this issue. Dana Settembre -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, May 11, 2011 11:16 AM To: Richard Cartun; Jayne Halli; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Her2 CAP survey Our staining was also light. I called CAP and they said to leave the result area blank, mark the bubble for "unable to analyze (11)", and to write an explanation on the last page. My pathologist is questioning why we wouldn't mark "specimen unsatisfactory (33)." What are others that had light staining doing and/or what have you been told by CAP? CAP also told me that they didn't have any replacement slides to send out. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, May 05, 2011 1:06 PM To: Jayne Halli; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Her2 CAP survey Everyone who experiencing problems with the CAP HER2 survey slides needs to contact the CAP to document the problem and request replacement slides. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Jayne Halli" 5/5/2011 3:01 PM >>> Cap validation Her2 slides we got one slide stained great, the other one did not, controls and cases all stained good. We use the Dako system. Jayne Halli HT,ASCP,QIHC Avera McKennan Histology 800 East 21st street Sioux Falls, SD 57117 (605)322-7142 my new e-mail address is jayne.halli@avera.org ----------------------------------------- Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From campbellj <@t> muhlbauerlab.com Wed May 11 10:52:38 2011 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Wed May 11 10:52:44 2011 Subject: [Histonet] IHC run print outs In-Reply-To: References: <201104291700.p3TH0nw3030579@nlpi138.prodigy.net> <986954.21674.qm@web81002.mail.mud.yahoo.com> <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> Message-ID: We had our in-house IT person create a print-out for us. We are a private lab so we are not inspected by CAP. That being said NYSDOH requires much of the same QC paperwork so we make do with what we have. On Wed, May 11, 2011 at 11:09 AM, WILLIAM DESALVO wrote: > > To meet CAP requirements, we print un reports and send send to the > pathologists with slides, they review, sign off and we keep for 2 yrs. All > QC paperwork is kept for 2 yrs. > > William DeSalvo, B.S., HTL(ASCP) > > > > > > Date: Wed, 11 May 2011 10:01:03 -0500 > > From: amber.mckenzie@gastrodocs.net > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] IHC run print outs > > > > > > > > Does everyone print out IHC run reports after each run? If so, how long > > do you keep them and are the pathologists supposed to sign off on them > > with their slides? > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 From Melissa.Kuhnla <@t> chsli.org Wed May 11 11:36:17 2011 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Wed May 11 11:36:22 2011 Subject: [Histonet] IHC run print outs In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> References: <201104291700.p3TH0nw3030579@nlpi138.prodigy.net><986954.21674.qm@web81002.mail.mud.yahoo.com> <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> Message-ID: Please let me know if there is some compliance issue we are missing...we are not CAP inspected. Only Joint Commission and NYSDOH. Our run logs are archived in the instrument software and can be produced on demand. As far as a doctor signoff, we use a disclaimer in all our reports that controls were run and stained appropriately. I think the doc's name on the report is signing off on the stain????????? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, May 11, 2011 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC run print outs Does everyone print out IHC run reports after each run? If so, how long do you keep them and are the pathologists supposed to sign off on them with their slides? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From cmiller <@t> physlab.com Wed May 11 11:51:46 2011 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed May 11 11:51:51 2011 Subject: [Histonet] IHC run print outs In-Reply-To: References: <201104291700.p3TH0nw3030579@nlpi138.prodigy.net> <986954.21674.qm@web81002.mail.mud.yahoo.com> <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> Message-ID: I submit them with the slides, The Dr will put his interpretation and then I keep them with my QA for 2+ years. Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From gu.lang <@t> gmx.at Wed May 11 11:53:38 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed May 11 11:53:46 2011 Subject: AW: [Histonet] IHC run print outs In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> References: <201104291700.p3TH0nw3030579@nlpi138.prodigy.net><986954.21674.qm@web81002.mail.mud.yahoo.com> <03C921A1EAF7F541B16543F6EC6A4B370418F396@giamail2.Gia.com> Message-ID: <0EAB83B0722246629788897B5DC27E13@dielangs.at> We have the Ventana Ultra Software. I don't print the reports, but save them on an extra file on the pc as pdfs. So in case of a software-breakdown, I can search the reports. It's just a QC-storage. I don't see much sense in printouts, because pathologists usually don't do troubleshooting and have no interest in lotnumbers. This is our job and we need then the information, if the machine had dispensed the right reagens. The doctors get the printed order with patient-label with the slides. Gudrun -----Ursprüngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Amber McKenzie Gesendet: Mittwoch, 11. Mai 2011 17:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] IHC run print outs Does everyone print out IHC run reports after each run? If so, how long do you keep them and are the pathologists supposed to sign off on them with their slides? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jayne.Halli <@t> avera.org Wed May 11 12:47:58 2011 From: Jayne.Halli <@t> avera.org (Jayne Halli) Date: Wed May 11 12:48:05 2011 Subject: [Histonet] Re: Her2 CAP survey In-Reply-To: Message-ID: When I called CAP they told me to record the results I got as long as my controls stained properly...and we did. Jayne Halli HT,ASCP,QIHC Avera McKennan Histology 800 East 21st street Sioux Falls, SD 57117 (605)322-7142 my new e-mail address is jayne.halli@avera.org -----Original Message----- From: Settembre, Dana [mailto:settembr@umdnj.edu] Sent: Wednesday, May 11, 2011 10:23 AM To: 'Laurie Colbert'; Richard Cartun; Jayne Halli; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Her2 CAP survey Laurie, when we called CAP they also told us to use Code 11. That's a GOOD point - specimen unsatisfactory is this issue. Dana Settembre -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, May 11, 2011 11:16 AM To: Richard Cartun; Jayne Halli; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Her2 CAP survey Our staining was also light. I called CAP and they said to leave the result area blank, mark the bubble for "unable to analyze (11)", and to write an explanation on the last page. My pathologist is questioning why we wouldn't mark "specimen unsatisfactory (33)." What are others that had light staining doing and/or what have you been told by CAP? CAP also told me that they didn't have any replacement slides to send out. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, May 05, 2011 1:06 PM To: Jayne Halli; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Her2 CAP survey Everyone who experiencing problems with the CAP HER2 survey slides needs to contact the CAP to document the problem and request replacement slides. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Jayne Halli" 5/5/2011 3:01 PM >>> Cap validation Her2 slides we got one slide stained great, the other one did not, controls and cases all stained good. We use the Dako system. Jayne Halli HT,ASCP,QIHC Avera McKennan Histology 800 East 21st street Sioux Falls, SD 57117 (605)322-7142 my new e-mail address is jayne.halli@avera.org ----------------------------------------- Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Debbie.Lake <@t> mgh.net Wed May 11 13:12:06 2011 From: Debbie.Lake <@t> mgh.net (Lake,Debbie) Date: Wed May 11 13:12:09 2011 Subject: [Histonet] HER2 CAP survey Message-ID: <8D6EDB72CECABB4A988C5BB087B61E4F01032E3D@mghemail1.mgh.net> Our staining was also light. I called CAP and they said to leave the result area blank, mark the bubble for "unable to analyze (11)", and to write an explanation on the last page. I asked if we could get another set of slides and she said no. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 662-4648 If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. From cbarone <@t> NEMOURS.ORG Wed May 11 14:40:39 2011 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Wed May 11 14:40:44 2011 Subject: [Histonet] Annexin V Message-ID: Histonetters: Looking for Annexin V Ab porcine reactive....anybody out there used Annexin V on piggies for histology? What is the scoop? I'd rather not re-invent the wheel. I am sure, someone has done ths.CB From kim.tournear <@t> yahoo.com Wed May 11 16:26:30 2011 From: kim.tournear <@t> yahoo.com (Yahoo) Date: Wed May 11 16:26:41 2011 Subject: [Histonet] Looking for Mike Riechenbach Message-ID: Long lost friend looking for Mike. He knows me as either Kim Mann or Kim Saunders from Tucson. Would love to here from him. Sent from the iPhone of Kim Tournear From pdunlop720 <@t> gmail.com Wed May 11 17:29:53 2011 From: pdunlop720 <@t> gmail.com (Patty Dunlop) Date: Wed May 11 17:29:58 2011 Subject: [Histonet] Job Opening for Histotech/Pathology Lab Manager (Monterey, CA) Message-ID: See job description below. If you want more info before applying, you can contact me or Helayne (her email is the "send resume to" email below). Thanks, Patty *Histotech Opening in Monterey, CA* *Position Title*: Histotech/Pathology Laboratory Manager *Location*: Physicians Office (GI) Pathology Laboratory in Monterey, CA *Shift*: Full Time, M ? F. Hours are somewhat flexible and must coordinate with pathologists? schedules. *Job Details:* We are looking for an experienced Histology Tech, who will be accountable for direct supervision and operation of our single-tech histology laboratory, and maintain high quality and cost-effective services. This position requires someone who is reliable, hard working, able to work independently and exhibits a strong attention to detail. The Histology tech will perform all duties related to the preparation of surgical GI specimens to be examined by pathologists. This includes but is not limited to:** ? Tissue Processing (microwave) ? Embedding ? Microtomy ? H&E (automated) and Special Staining (manual) ? Cover-slipping (manual) Also performs all duties related to laboratory management, including equipment care and maintenance, inventory/ordering, archiving of specimens, recordkeeping, etc. *We are a formalin-free and xylene-free laboratory. *Job Requirements:* Ability to work with no supervision HT or HTL (ASCP) certification required At least 3 years experience working in histology with little or no supervision Available to start in June 2011 *Compensation: * Competitive salary, health benefits and 401(k) *To apply*: *Please submit resume t*o hwilliams@montereygi.com From DSiena <@t> statlab.com Wed May 11 17:30:11 2011 From: DSiena <@t> statlab.com (Debra Siena) Date: Wed May 11 17:30:18 2011 Subject: [Histonet] DMSO in Tissue processor Message-ID: Hi Everyone, I was hoping that I could ask a question about the use of paraffins with DMSO in tissue processors. I was wondering if there are any tissue processors that recommend or specify that paraffins containing DMSO not be used. I am not aware of any of the older versions but just wondered about the newer microwave processors and other newer versions of processors now on the market. Thanks for all your help! Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsiena@statlab.com | www.statlab.com From ctam <@t> stanford.edu Wed May 11 19:09:17 2011 From: ctam <@t> stanford.edu (Cheuk Ho Tam) Date: Wed May 11 19:09:21 2011 Subject: [Histonet] hematoxylin and DAB/Alkaline Phosphatase staining In-Reply-To: <1890808368.160871.1305158653533.JavaMail.root@zm08.stanford.edu> Message-ID: <788921090.161082.1305158957357.JavaMail.root@zm08.stanford.edu> Dear Histonet Members, I am currently staining 20 um cryostat sections of brain tissue and visualizing with DAB along with hematoxylin counterstain. For sections stained with the same concentration of primary antibody, I seem to get lighter DAB staining for slides with hematoxylin than ones without. Is it possible that hematoxylin can interfere with DAB staining? I am using the ABC kit from for DAB and pre-made Hematoxylin QS, both from Vector Labs. On a different note, I've noticed some sponginess in occasional sets of slides. I'm wondering what can be the cause. A labmate told me that it's probably something wrong with the fixation or cryoprotection. I've treated the brain tissue the same for the batch, and only one slide set had sponginess. I fix in 4% PFA for 24 hours, cryoprotect with 30% sucrose for 1-5 days, and mount in OCT. Any suggestions would be appreciated. Thank you for your help. Sincerely, Cheuk From candice_camille <@t> yahoo.com Thu May 12 09:20:18 2011 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Thu May 12 09:20:22 2011 Subject: [Histonet] H and E tumor Message-ID: <424315.91931.qm@web125416.mail.ne1.yahoo.com> Hi guys, ??? I was wondering if anyone had a picture of a tumor stained with H and E. Please?reply with it if you do. It does not matter the tissue. Thanks ?I remain yours truely, Candice Camille From rjbuesa <@t> yahoo.com Thu May 12 09:27:21 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 12 09:27:29 2011 Subject: [Histonet] DMSO in Tissue processor In-Reply-To: References: Message-ID: <455696.88493.qm@web65709.mail.ac4.yahoo.com> DMSO is always used in very small amounts and I am not aware of any restrictions about its use with any type of tissue processor. Ren? J. From: Debra Siena To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, May 11, 2011 6:30 PM Subject: [Histonet] DMSO in Tissue processor Hi Everyone, I was hoping that I could ask a question about the use of paraffins with DMSO in tissue processors.? I was wondering if there are any tissue processors that recommend or specify that paraffins containing DMSO not be used.? I am not aware of any of the older versions but just wondered about the newer microwave processors and other newer versions of processors now on the market.? Thanks for all your help! Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010? x229 | Fax: 972-436-1369 dsiena@statlab.com | www.statlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Carmen.M.Garcia <@t> uv.es Thu May 12 10:02:53 2011 From: Carmen.M.Garcia <@t> uv.es (Carmen Maria Garcia Pascual) Date: Thu May 12 10:03:00 2011 Subject: [Histonet] filopodia of Endothelial cells in corpus luteum in mouse ovary In-Reply-To: <788921090.161082.1305158957357.JavaMail.root@zm08.stanford.edu> References: <788921090.161082.1305158957357.JavaMail.root@zm08.stanford.edu> Message-ID: <5783646291carmaga6@uv.es> Dear Histonet members: I am interesting in see the filopodia of the endothelial cells in the corpus luteum of mouse. I have stained 25um cryostat sections of ovary with an antibody agaisnt CD31 (PECAM) and alexa fluor and see it in the confocal microscope, but I haven't succes. someone can tell me what can I do? than you so much!!!!!! -- ******************************************************* Carmen Mar?a Garc?a Pascual FIVI/INCLIVA/Facultad de Medicina Universidad de Valencia Departamento de P.O.G (Laboratorios) 96.386.40.48 Avd. Blasco Iba?ez 17 46010, Valencia ******************************************************* From jhill <@t> vet.k-state.edu Thu May 12 11:32:23 2011 From: jhill <@t> vet.k-state.edu (Jennifer Hill) Date: Thu May 12 11:32:28 2011 Subject: [Histonet] Lab supervisor/histotechnician job opening Message-ID: <8AA2173DC209CA438077A832FF98BD7F02A32D39@VETMXHT.ads.vet.k-state.edu> Kansas State University in Manhattan, KS, is looking for a lab supervisor/histotechnician/immunohistochemistry candidate to work in a veterinary diagnostic histology/immunohistochemistry laboratory in the College of Veterinary Medicine. Manhattan, KS has been recognized as one of the top places to live within the past few years. You can check out this position at the following link; scroll down to the 'Microbiologist III' position to find the specifics. http://www.k-state.edu/hr/employment/vac.html For direct contact: Dr. Brad DeBey (debey@vet.k-state.edu) From PMonfils <@t> Lifespan.org Thu May 12 11:41:37 2011 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu May 12 11:41:43 2011 Subject: [Histonet] DMSO in Tissue processor In-Reply-To: References: Message-ID: <4EBFF65383B74D49995298C4976D1D5E0827169C@LSRIEXCH1.lsmaster.lifespan.org> I never heard of any such recommendations. DMSO doesn't react with plastics or metals, and those are the only surfaces the embedding medium comes into contact with, so I wouldn't expect any problems. In any case, from a personal perspective, I used Paraplast Plus, which contains DMSO, in my processors for many years, and never had a problem. From Nacaela.Johnson <@t> USONCOLOGY.COM Thu May 12 11:51:15 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Thu May 12 11:51:17 2011 Subject: [Histonet] nuclear bubbling Message-ID: <71882EED22A283429E8424513A22922D0CD1DC@txhous1eb015.uson.usoncology.int> Has anyone one had problems with nuclear bubbling? I have read about two different causes. (1) Formalin itself is known to cause the issue and (2) heating the tissue at a high temp (70 degrees C or above) while drying. The latter is definitely not happening, but I do use formalin. I am in the process of changing the type of formalin that is used during collection. Does anyone have any other suggestions? Has this problem occurred for any other reason than I have already pointed out? It seems to be worse in the surgical needle biopsies than in the bone marrow biopsies. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From azdudley <@t> hotmail.com Thu May 12 12:54:30 2011 From: azdudley <@t> hotmail.com (anita dudley) Date: Thu May 12 12:54:34 2011 Subject: [Histonet] ventana validation Message-ID: would someone send me a copy of their validation that they do for the dab or red ultra view kits? I am not sure how to do this on the machine, do you do a control one one kit and the same on the new? just not sure what to do. thanks so much in advance anita dudley providence hosp mobile alabama From gu.lang <@t> gmx.at Thu May 12 13:10:24 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu May 12 13:17:11 2011 Subject: AW: [Histonet] nuclear bubbling In-Reply-To: <71882EED22A283429E8424513A22922D0CD1DC@txhous1eb015.uson.usoncology.int> References: <71882EED22A283429E8424513A22922D0CD1DC@txhous1eb015.uson.usoncology.int> Message-ID: http://decapoda.nhm.org/pdfs/1505/1505.pdf Here you can find an assay about formalin fixation. On page 851 there is a description of nuclear and cytoplasmic bubbling found few minutes after addition of formaldehyd-solution to cultured cells. My interpretation is, that too short fixation makes the bubbling visible in the tissue slides. And that after adequate duration of fixation the bubbles are gone somehow. What do you think? Gudrun -----Ursprüngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Johnson, Nacaela Gesendet: Donnerstag, 12. Mai 2011 18:51 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] nuclear bubbling Has anyone one had problems with nuclear bubbling? I have read about two different causes. (1) Formalin itself is known to cause the issue and (2) heating the tissue at a high temp (70 degrees C or above) while drying. The latter is definitely not happening, but I do use formalin. I am in the process of changing the type of formalin that is used during collection. Does anyone have any other suggestions? Has this problem occurred for any other reason than I have already pointed out? It seems to be worse in the surgical needle biopsies than in the bone marrow biopsies. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Doug.Showers <@t> propath.com Thu May 12 13:35:28 2011 From: Doug.Showers <@t> propath.com (Doug Showers) Date: Thu May 12 13:35:02 2011 Subject: [Histonet] Job Opening in Dallas, Texas Message-ID: <82C7248978CB50469FD6BA68EBBEFE6704FEF3DC@exchange.propathlab.com> ProPath, a progressive, CAP accredited, high volume, pathology practice, located in Dallas, Texas, is seeking a Histotechnologist. The hours for this position are Monday through Friday, 2:00 pm to 10:30 pm. In this position you will be responsible for embedding tissue specimens, microtomy of paraffin-embedded tissue, operation of automated stainers and coverslippers, equipment maintenance and record retention. The ideal candidate will have a high school diploma or equivalent. We prefer, HT, HTL (ASCP) registered or eligible. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! EOE For consideration send resume to: ProPath, Human Resources 1355 River Bend Drive Dallas, TX 75247 FAX: 214/237-1825 Job-Line: 214/237-1775 Email address: jobs@propath.com Website: www.propath.com Doug Showers, MS, HT Histology Manager ProPath 1355 River Bend Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com The information contained in this message may be privileged and confidential. If you are NOT the intended recipient, please notify the sender immediately with a copy to Postmaster@propath.com and destroy this message. From LSebree <@t> uwhealth.org Thu May 12 13:44:52 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu May 12 13:44:56 2011 Subject: [Histonet] ventana validation In-Reply-To: References: Message-ID: We run a control with the new kit and compare it with a control that has been run with the current kit (kept in a detection kit QC file). We keep it as simple as possible with the least amount of cost we can manage. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Thursday, May 12, 2011 12:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ventana validation would someone send me a copy of their validation that they do for the dab or red ultra view kits? I am not sure how to do this on the machine, do you do a control one one kit and the same on the new? just not sure what to do. thanks so much in advance anita dudley providence hosp mobile alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mary.helie <@t> yale.edu Thu May 12 13:48:22 2011 From: mary.helie <@t> yale.edu (Mary Helie) Date: Thu May 12 13:48:33 2011 Subject: [Histonet] Re: Histonet Digest, Vol 89, Issue 34 In-Reply-To: <201104281701.p3SH1nPc020197@mr2.its.yale.edu> References: <201104281701.p3SH1nPc020197@mr2.its.yale.edu> Message-ID: <4DCC2B76.2050408@yale.edu> We did not plan well so getting the EBER up and running was priority one. I just entered the 4 ml. Working up the protocol --what was best in our hands was protease 2- 4 mins, probe, then 2 -8 min stringency washes at 37 degrees. Perhaps now I can work on diluting it. If you get some info on that, I would appreciate it. Thank you On 4/28/11 1:01 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Diff-Quik stain (Robert Richmond) > 2. chicken immunohistochemistry (mohamed abd el razik) > 3. Re: MSH 2011 Symposium (David Kemler) > 4. IMEB (Sheila Haas) > 5. RE: IMEB (Kaye Ryan) > 6. RE: IMEB (sgoebel@mirnarx.com) > 7. Re: IMEB (Sheila Haas) > 8. RE: IMEB (Bartlett, Jeanine (CDC/OID/NCEZID)) > 9. RE: IMEB (Rene J Buesa) > 10. Ventana Probes (Angela Bitting) > 11. Re: Ventana Probes (Angela Bitting) > 12. Re: Ventana Probes (Rene J Buesa) > 13. Re: Ventana Probes (Rene J Buesa) > 14. TNF alpha antibody (Michele Wich) > 15. RE: TNF alpha antibody (Liz Chlipala) > 16. TNF-alpha (Liz Chlipala) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 27 Apr 2011 21:47:10 -0400 > From: Robert Richmond > Subject: [Histonet] Re: Diff-Quik stain > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=ISO-8859-1 > > Diff-Quik is a trade name - belongs to whatever Scientific Products is > called this week. It's often used generically for all two-stain > Romanowsky type stains, since there isn't a really satisfactory > generic name for them. > > I've used several generic stains with separate eosin ("xanthine") and > blue ("thiazine") dye baths, and generally found them satisfactory. > But you do need to try them out for yourself. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > > > ------------------------------ > > Message: 2 > Date: Thu, 28 Apr 2011 01:10:06 -0700 (PDT) > From: mohamed abd el razik > Subject: [Histonet] chicken immunohistochemistry > To: Histonet@lists.utsouthwestern.edu > Message-ID:<496385.41591.qm@web112602.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > dear all > i'm looking for vendors or companies that we could order some anti chicken antibodies (primary& secondary). speacially for CD4& CD8 > i'm new in that feild and need your help. > > mohamed > > > ------------------------------ > > Message: 3 > Date: Thu, 28 Apr 2011 03:12:07 -0700 (PDT) > From: David Kemler > Subject: Re: [Histonet] MSH 2011 Symposium > To: Fellow HistoNetters > Message-ID:<177783.69813.qm@web120605.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi Amber - > > I have the MSH Symposium scheduled to begin promos on May 8th's HistoTALK. > Hopefully, the announcements will help get listeners attention and get them to > attend. I'll be sure to mention your email on the show. :- ) > > Yours, > Dave > www.HistoTALK.com > > > > > ________________________________ > From: Amber McKenzie > Cc: Histonet list serv. > Sent: Wed, April 27, 2011 12:08:14 PM > Subject: [Histonet] MSH 2011 Symposium > > > > > If anyone is interested in attending the annual Mississippi Society for > Histotechnology Symposium June 24-26th in Hattiesburg, MS, please > contact me directly and I can email you a program! We'd love to have > you :) > > Amber McKenzie, BS, HT(ASCP) > MSH Secretary > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 4 > Date: Thu, 28 Apr 2011 04:17:14 -0700 (PDT) > From: Sheila Haas > Subject: [Histonet] IMEB > To: histonet@lists.utsouthwestern.edu > Message-ID:<967305.23545.qm@web161712.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Thanks to everyone for the feedback on IMEB. It appears there have only been a > couple of successful recent transactions with them. Most of the feedback I > received was not favorable so it appears the company is in > a good bit of disarray for reasons unknown. At this point, I've not gotten > a response from the owner regarding the return of our entire deposit but if > he actually comes through, I will let you all know. In the mean time, be leary > of future transactions with them. Thanks again for the feedback. > > Sheila Haas > Laboratory Supervisor > MicroPath Laboratories, Inc. > > ------------------------------ > > Message: 5 > Date: Thu, 28 Apr 2011 10:32:22 -0400 > From: "Kaye Ryan" > Subject: RE: [Histonet] IMEB > To: "Sheila Haas", > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible tha! > t people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. > > Kaye Ryan > Histology Manager > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas > Sent: Thursday, April 28, 2011 7:17 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IMEB > > Thanks to everyone for the feedback on IMEB. It appears there have only been a > couple of successful recent transactions with them. Most of the feedback I > received was not favorable so it appears the company is in > a good bit of disarray for reasons unknown. At this point, I've not gotten > a response from the owner regarding the return of our entire deposit but if > he actually comes through, I will let you all know. In the mean time, be leary > of future transactions with them. Thanks again for the feedback. > > Sheila Haas > Laboratory Supervisor > MicroPath Laboratories, Inc. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 6 > Date: Thu, 28 Apr 2011 09:46:09 -0500 > From: > Subject: RE: [Histonet] IMEB > To:,, > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > I agree Kaye!! IMEB has always gone above and beyond to help me with anything I have ever needed. They even had one of their techs call and help me troubleshoot some equipment that wasn't even theirs! I can never say enough awesome things about Denise either!! She is AWESOME!! Keeps in contact and tries to help out in any way she can!! I bought a used processor from them and a few months later it had an issue. They sent someone to my lab (on their dime by the way) who fixed it on the spot! From the time I called to the time it was fixed was less than 2 days!! It was an old VIP and the repair tech even made his own diagnostic equipment and repair parts! I would recommend this company to anyone (and have!!). It is a shame that one person's bad experience has been broadcast over the histonet for the world to see. With anything in this world there is always going to be one person who has a bad taste in their mouth about something. Because people always yell out the ! > bad and rarely yell out the good here I go...IMEB IS A GREAT COMPANY!!!! > > I will now step down off my box =) Have a Happy Thursday Histo-hotties!! > > Sarah Goebel, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan > Sent: Thursday, April 28, 2011 9:32 AM > To: Sheila Haas; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] IMEB > > I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible tha! > t people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. > > Kaye Ryan > Histology Manager > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas > Sent: Thursday, April 28, 2011 7:17 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IMEB > > Thanks to everyone for the feedback on IMEB. It appears there have only been a > couple of successful recent transactions with them. Most of the feedback I > received was not favorable so it appears the company is in > a good bit of disarray for reasons unknown. At this point, I've not gotten > a response from the owner regarding the return of our entire deposit but if > he actually comes through, I will let you all know. In the mean time, be leary > of future transactions with them. Thanks again for the feedback. > > Sheila Haas > Laboratory Supervisor > MicroPath Laboratories, Inc. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Thu, 28 Apr 2011 07:48:00 -0700 (PDT) > From: Sheila Haas > Subject: Re: [Histonet] IMEB > To: Kaye Ryan, histonet@lists.utsouthwestern.edu > Message-ID:<829976.69833.qm@web161717.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Kaye, > I was telling of my situation and asked if others had recent problems. I > received many responses with two being positive and most of the others negative > (a couple were offering suggestions on how to handle). I have heard this company > was very reputable which is why I chose to do business with them in the first > place but have since heard that recently things have been quite rocky. I do > feel, at this point, that others should be leary before ordering from this > company. I'd hate for someone else to be in the same situation as we are, > particularly during these difficult economic times (we are out a large sum of > money at this point). I also stated that if the issue is resolved, I would let > everyone know but up until now it has not been. > > You are right, we did not have an account with IMEB but what was promised in the > beginning is not what played out. I have been in this field for a very long time > and realize how the accounting works but I also realize that a full refund is > not unreasonable given I jumped through every hoop they requested I jump > through. Keep in mind, restocking fees typically occur when items are already > shipped or delivered to the customer and they return them. This is not the case > here, they have both the item and our money. Again, if this issue is resolved to > my satisfaction, I will gladly let everyone know. > > > > Sheila Haas > > > > > > > ________________________________ > From: Kaye Ryan > To: Sheila Haas; histonet@lists.utsouthwestern.edu > Sent: Thu, April 28, 2011 10:32:22 AM > Subject: RE: [Histonet] IMEB > > I usually do not respond to postings because I have seen people that may not > agree with a posting get "roasted" by others but I felt like I had to chance it > with this posting. I have had many excellent transactions with IMEB and would > recommend them to anyone. Their products are trustworthy and their prices are > excellent compared to other companies that sell the same products. I have known > the owner and several of the reps for many years and have never heard of an > incident like this. I too, had to put a deposit down when I made my first order > because we did not have an established account with them. I am assuming that > you did not have an account already established since they were checking your > credit status? It does take time to fill out the paper work and for them to > check your credit references. It is that way with almost any new company that > you place a large order with. In this day and time "trust" is a hard thing to > come by on a new account. It is terrible that people that may not have used > them now have a negative thought in their minds about the company. We all have > at some time something that we can find not quite to our liking about any > company and I think we need to be careful when we are saying statements like " > It appears there have only been a couple of successful recent transactions with > them". This can be interpreted by some to mean that there have been many > negative interactions so stay clear of them. Maybe a lot of their customers do > not reply to posting or do not even subscribe to the histonet. > > Kaye Ryan > Histology Manager > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas > Sent: Thursday, April 28, 2011 7:17 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IMEB > > Thanks to everyone for the feedback on IMEB. It appears there have only been a > couple of successful recent transactions with them. Most of the feedback I > received was not favorable so it appears the company is in > a good bit of disarray for reasons unknown. At this point, I've not gotten > a response from the owner regarding the return of our entire deposit but if > he actually comes through, I will let you all know. In the mean time, be leary > of future transactions with them. Thanks again for the feedback. > > Sheila Haas > Laboratory Supervisor > MicroPath Laboratories, Inc. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 8 > Date: Thu, 28 Apr 2011 14:48:01 +0000 > From: "Bartlett, Jeanine (CDC/OID/NCEZID)" > Subject: RE: [Histonet] IMEB > To: "sgoebel@mirnarx.com", "kryan@nfderm.com" > , "micropathlabs@yahoo.com" > , "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > It would clear things up quite a bit if they would simply return her call. > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com > Sent: Thursday, April 28, 2011 10:46 AM > To: kryan@nfderm.com; micropathlabs@yahoo.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] IMEB > > I agree Kaye!! IMEB has always gone above and beyond to help me with anything I have ever needed. They even had one of their techs call and help me troubleshoot some equipment that wasn't even theirs! I can never say enough awesome things about Denise either!! She is AWESOME!! Keeps in contact and tries to help out in any way she can!! I bought a used processor from them and a few months later it had an issue. They sent someone to my lab (on their dime by the way) who fixed it on the spot! From the time I called to the time it was fixed was less than 2 days!! It was an old VIP and the repair tech even made his own diagnostic equipment and repair parts! I would recommend this company to anyone (and have!!). It is a shame that one person's bad experience has been broadcast over the histonet for the world to see. With anything in this world there is always going to be one person who has a bad taste in their mouth about something. Because people always yell out the ! > bad and rarely yell out the good here I go...IMEB IS A GREAT COMPANY!!!! > > I will now step down off my box =) Have a Happy Thursday Histo-hotties!! > > Sarah Goebel, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan > Sent: Thursday, April 28, 2011 9:32 AM > To: Sheila Haas; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] IMEB > > I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible tha! > t people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. > > Kaye Ryan > Histology Manager > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas > Sent: Thursday, April 28, 2011 7:17 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IMEB > > Thanks to everyone for the feedback on IMEB. It appears there have only been a > couple of successful recent transactions with them. Most of the feedback I > received was not favorable so it appears the company is in > a good bit of disarray for reasons unknown. At this point, I've not gotten > a response from the owner regarding the return of our entire deposit but if > he actually comes through, I will let you all know. In the mean time, be leary > of future transactions with them. Thanks again for the feedback. > > Sheila Haas > Laboratory Supervisor > MicroPath Laboratories, Inc. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Thu, 28 Apr 2011 07:51:39 -0700 (PDT) > From: Rene J Buesa > Subject: RE: [Histonet] IMEB > To: Sheila Haas, > histonet@lists.utsouthwestern.edu, Kaye Ryan > Message-ID:<119316.93664.qm@web65711.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Kaye: > In no way this posting is aimed at "roasting" yours, not at all, it is just to explain MY philosophy when participating in HistoNet. > When I read a posting first I consider if I have something useful to contribute and if that is the case I post my answer and I really do not care if it will follow a "consensus", it is my answer based on my experience and from the start I consider that I may be wrong or that others' answers will reflect reality in a better way. > The fact that you may have a good commercial experience with IMEB in absolutely no way excludes the possibility that others' experiences could have been disastrous. > Additionally, it does not really matter how others have related to this company if the one posting the initial query has had a bad experience. > Summing up do as I do: post your answer if you consider will help, and absolutely disregard what others may think of it, and never try to impose your criterion. > Ren? J. > > --- On Thu, 4/28/11, Kaye Ryan wrote: > > > From: Kaye Ryan > Subject: RE: [Histonet] IMEB > To: "Sheila Haas", histonet@lists.utsouthwestern.edu > Date: Thursday, April 28, 2011, 10:32 AM > > > I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new > account. It is terrible that people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. > > Kaye Ryan > Histology Manager > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas > Sent: Thursday, April 28, 2011 7:17 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IMEB > > Thanks to everyone for the feedback on IMEB. It appears there have only been a > couple of successful recent transactions with them. Most of the feedback I > received was not favorable so it appears the company is in > a good bit of disarray for reasons unknown. At this point, I've not gotten > a response from the owner regarding the return of our entire deposit but if > he actually comes through, I will let you all know. In the mean time, be leary > of future transactions with them. Thanks again for the feedback. > > Sheila Haas > Laboratory Supervisor > MicroPath Laboratories, Inc. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 10 > Date: Thu, 28 Apr 2011 11:03:43 -0400 > From: "Angela Bitting" > Subject: [Histonet] Ventana Probes > To: "histonet" > Message-ID:<4DB9498F.2B7F.00C9.1@geisinger.edu> > Content-Type: text/plain; charset="us-ascii" > > Ok. Ventana is on my last nerve with this new probe configuration of theirs. > I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. > So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. > > If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? > Sorry for the Thursday rant....anyway Happy Lab Week everyone!!! > > Angela Bitting, HT(ASCP), QIHC > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > -------------- next part -------------- > BEGIN:VCARD > VERSION:2.1 > X-GWTYPE:USER > FN:Bitting, Angela > TEL;WORK:570-271-6844 > ORG:;Histology > EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu > N:Bitting;Angela > END:VCARD > > > ------------------------------ > > Message: 11 > Date: Thu, 28 Apr 2011 11:10:45 -0400 > From: "Angela Bitting" > Subject: Re: [Histonet] Ventana Probes > To: "Angela Bitting", "histonet" > > Message-ID:<4DB94B35.2B7F.00C9.1@geisinger.edu> > Content-Type: text/plain; charset=US-ASCII > > Sorry folks, in my impaired state of mind I made a boo boo. I need 5-6 ml to do 50 tests. If the dispenses are still 100 ul each. Any thoughts? > > >>>> "Angela Bitting" 4/28/2011 11:03 AM>>> >>>> > Ok. Ventana is on my last nerve with this new probe configuration of theirs. > I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. > So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. > > If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? > Sorry for the Thursday rant....anyway Happy Lab Week everyone!!! > > Angela Bitting, HT(ASCP), QIHC > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > > > ------------------------------ > > Message: 12 > Date: Thu, 28 Apr 2011 08:13:18 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Ventana Probes > To: histonet, Angela Bitting > > Message-ID:<433054.26360.qm@web65712.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Before adding vodka, take some "sips". > Ren? J. > > --- On Thu, 4/28/11, Angela Bitting wrote: > > > From: Angela Bitting > Subject: [Histonet] Ventana Probes > To: "histonet" > Date: Thursday, April 28, 2011, 11:03 AM > > > Ok. Ventana is on my last nerve with this new probe configuration of theirs. > I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. > So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. > > If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? > Sorry for the Thursday rant....anyway Happy Lab Week everyone!!! > > Angela Bitting, HT(ASCP), QIHC > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > -----Inline Attachment Follows----- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 13 > Date: Thu, 28 Apr 2011 08:17:16 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Ventana Probes > To: Angela Bitting, histonet > , Angela Bitting > > Message-ID:<533509.43351.qm@web65712.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > At 100?L per dispense, to do 50 tests you will need > 50 x 100?L = 5000 ?L = 5 ml > You do need to dilute it > Ren? J. > > --- On Thu, 4/28/11, Angela Bitting wrote: > > > From: Angela Bitting > Subject: Re: [Histonet] Ventana Probes > To: "Angela Bitting", "histonet" > Date: Thursday, April 28, 2011, 11:10 AM > > > Sorry folks, in my impaired state of mind I made a boo boo. I need 5-6 ml to do 50 tests. If the dispenses are still 100 ul each. Any thoughts? > > >>>> "Angela Bitting" 4/28/2011 11:03 AM>>> >>>> > Ok. Ventana is on my last nerve with this new probe configuration of theirs. > I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. > So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. > > If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? > Sorry for the Thursday rant....anyway Happy Lab Week everyone!!! > > Angela Bitting, HT(ASCP), QIHC > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 14 > Date: Thu, 28 Apr 2011 11:07:42 -0500 > From: "Michele Wich" > Subject: [Histonet] TNF alpha antibody > To: > Message-ID: > <62A8156F8071C8439080D626DF8C33A60174E398@wave-mail.7thwave.local> > Content-Type: text/plain; charset="us-ascii" > > Can anyone recommend a good TNF-alpha antibody for frozen mouse tissue? > > Any suggestions are greatly appreciated! > > > > ------------------------------ > > Message: 15 > Date: Thu, 28 Apr 2011 10:15:48 -0600 > From: "Liz Chlipala" > Subject: RE: [Histonet] TNF alpha antibody > To: "Michele Wich", > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > We have used the antibody from abcam on FFPE mouse tissue with good > success, have not tired it on frozen sections > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, Colorado 80308 > office (303) 682-3949 > fax (303) 682-9060 > www.premierlab.com > > > Ship to Address: > 1567 Skyway Drive, Unit E > Longmont, Colorado 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele > Wich > Sent: Thursday, April 28, 2011 10:08 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] TNF alpha antibody > > Can anyone recommend a good TNF-alpha antibody for frozen mouse tissue? > > Any suggestions are greatly appreciated! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 16 > Date: Thu, 28 Apr 2011 10:30:28 -0600 > From: "Liz Chlipala" > Subject: [Histonet] TNF-alpha > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > I was incorrect in my earlier post, we actually use the antibody from > Sigma for TNF- alpha, I was thinking of IL-6 that's the antibody we get > from abcam > > > > Liz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > > Manager > > Premier Laboratory, LLC > > PO Box 18592 > > Boulder, Colorado 80308 > > office (303) 682-3949 > > fax (303) 682-9060 > > www.premierlab.com > > > > > > Ship to Address: > > 1567 Skyway Drive, Unit E > > Longmont, Colorado 80504 > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 89, Issue 34 > **************************************** > > > From azdudley <@t> hotmail.com Thu May 12 14:01:48 2011 From: azdudley <@t> hotmail.com (anita dudley) Date: Thu May 12 14:01:53 2011 Subject: [Histonet] ventana validation In-Reply-To: References: , Message-ID: thanks linda I think that sounds good, I am going to do that. anita > Subject: RE: [Histonet] ventana validation > Date: Thu, 12 May 2011 13:44:52 -0500 > From: LSebree@uwhealth.org > To: azdudley@hotmail.com; histonet@lists.utsouthwestern.edu > > We run a control with the new kit and compare it with a control that has > been run with the current kit (kept in a detection kit QC file). We > keep it as simple as possible with the least amount of cost we can > manage. > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita > dudley > Sent: Thursday, May 12, 2011 12:55 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] ventana validation > > > would someone send me a copy of their validation that they do for the > dab or red ultra view kits? I > am not sure how to do this on the machine, do you do a control one one > kit and the same on the new? > just not sure what to do. thanks so much in advance > > anita dudley > providence hosp > mobile alabama > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 12 14:40:49 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 12 14:40:52 2011 Subject: [Histonet] nuclear bubbling In-Reply-To: <71882EED22A283429E8424513A22922D0CD1DC@txhous1eb015.uson.usoncology.int> References: <71882EED22A283429E8424513A22922D0CD1DC@txhous1eb015.uson.usoncology.int> Message-ID: <214219.31758.qm@web65715.mail.ac4.yahoo.com> Nacaela: Heat by itself is not the cause, but the heat applied to sections that have not been properly drained and still have water left between them and the slide, is. You have to drain the sections properly before heating them. Using formalin is not the cause. Ren? J. From: "Johnson, Nacaela" To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 12, 2011 12:51 PM Subject: [Histonet] nuclear bubbling Has anyone one had problems with nuclear bubbling?? I have read about two different causes.? (1) Formalin itself is known to cause the issue and (2) heating the tissue at a high temp (70 degrees C or above) while drying.? The latter is definitely not happening, but I do use formalin. I am in the process of changing the type of formalin that is used during collection.? Does anyone have any other suggestions?? Has this problem occurred for any other reason than I have already pointed out?? It seems to be worse in the surgical needle biopsies than in the bone marrow biopsies. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office:? 913-234-0576 Fax:? 913-433-7639 Email:? Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nacaela.Johnson <@t> USONCOLOGY.COM Thu May 12 14:50:08 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Thu May 12 14:50:12 2011 Subject: [Histonet] nuclear bubbling In-Reply-To: <214219.31758.qm@web65715.mail.ac4.yahoo.com> References: <71882EED22A283429E8424513A22922D0CD1DC@txhous1eb015.uson.usoncology.int> <214219.31758.qm@web65715.mail.ac4.yahoo.com> Message-ID: <71882EED22A283429E8424513A22922D0CD1E2@txhous1eb015.uson.usoncology.int> So there really isn't a point in using an oven to dry the slides? I currently dry my slides for an hour at 55-60 degrees C. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, May 12, 2011 2:41 PM To: Johnson, Nacaela; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] nuclear bubbling Nacaela: Heat by itself is not the cause, but the heat applied to sections that have not been properly drained and still have water left between them and the slide, is. You have to drain the sections properly before heating them. Using formalin is not the cause. Ren? J. From: "Johnson, Nacaela" To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 12, 2011 12:51 PM Subject: [Histonet] nuclear bubbling Has anyone one had problems with nuclear bubbling?? I have read about two different causes.? (1) Formalin itself is known to cause the issue and (2) heating the tissue at a high temp (70 degrees C or above) while drying.? The latter is definitely not happening, but I do use formalin. I am in the process of changing the type of formalin that is used during collection.? Does anyone have any other suggestions?? Has this problem occurred for any other reason than I have already pointed out?? It seems to be worse in the surgical needle biopsies than in the bone marrow biopsies. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office:? 913-234-0576 Fax:? 913-433-7639 Email:? Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From candice_camille <@t> yahoo.com Thu May 12 15:09:34 2011 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Thu May 12 15:09:37 2011 Subject: [Histonet] H and E tumor In-Reply-To: <4DCBF513.7400.0077.1@harthosp.org> References: <424315.91931.qm@web125416.mail.ne1.yahoo.com> <4DCBF513.7400.0077.1@harthosp.org> Message-ID: <542074.42108.qm@web125414.mail.ne1.yahoo.com> I did!!!! Thanks! ?I remain yours truely, Candice Camille ________________________________ From: Richard Cartun To: Candice Smoots Sent: Thu, May 12, 2011 1:56:19 PM Subject: Re: [Histonet] H and E tumor Did you get what you needed? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT? 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Candice Smoots 5/12/2011 10:20 AM >>> Hi guys, ? I was wondering if anyone had a picture of a tumor stained with H and E. Please reply with it if you do. It does not matter the tissue. Thanks I remain yours truely, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nacaela.Johnson <@t> USONCOLOGY.COM Thu May 12 15:25:11 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Thu May 12 15:26:14 2011 Subject: [Histonet] nuclear bubbling In-Reply-To: <71882EED22A283429E8424513A22922D0CD1E2@txhous1eb015.uson.usoncology.int> References: <71882EED22A283429E8424513A22922D0CD1DC@txhous1eb015.uson.usoncology.int><214219.31758.qm@web65715.mail.ac4.yahoo.com> <71882EED22A283429E8424513A22922D0CD1E2@txhous1eb015.uson.usoncology.int> Message-ID: <71882EED22A283429E8424513A22922D0CD1E3@txhous1eb015.uson.usoncology.int> Another individual called me and commented further, which clarified your statement. It is actually poor lab practice to combine the drying/baking. These steps should really be done separately so as to not "trap" the water under the tissue. I have noticed bubbles of water on the bottom of my slides that are encapsulated in wax. I assume this is also happening under the tissue as well, therefore affecting the nuclear morphology. I had been taught to speed up the process by placing them in the oven directly after cutting. Decreasing the TAT was all they really cared about. Fortunately where I work now chooses quality over quantity and I can adjust my protocol to get rid of the artifact. Thank you all for your comments and clarity. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Nacaela Sent: Thursday, May 12, 2011 2:50 PM To: Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nuclear bubbling So there really isn't a point in using an oven to dry the slides? I currently dry my slides for an hour at 55-60 degrees C. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, May 12, 2011 2:41 PM To: Johnson, Nacaela; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] nuclear bubbling Nacaela: Heat by itself is not the cause, but the heat applied to sections that have not been properly drained and still have water left between them and the slide, is. You have to drain the sections properly before heating them. Using formalin is not the cause. Ren? J. From: "Johnson, Nacaela" To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 12, 2011 12:51 PM Subject: [Histonet] nuclear bubbling Has anyone one had problems with nuclear bubbling?? I have read about two different causes.? (1) Formalin itself is known to cause the issue and (2) heating the tissue at a high temp (70 degrees C or above) while drying.? The latter is definitely not happening, but I do use formalin. I am in the process of changing the type of formalin that is used during collection.? Does anyone have any other suggestions?? Has this problem occurred for any other reason than I have already pointed out?? It seems to be worse in the surgical needle biopsies than in the bone marrow biopsies. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office:? 913-234-0576 Fax:? 913-433-7639 Email:? Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Thu May 12 15:46:21 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu May 12 15:46:26 2011 Subject: [Histonet] nuclear bubbling In-Reply-To: <71882EED22A283429E8424513A22922D0CD1E3@txhous1eb015.uson.usoncology.int> References: <71882EED22A283429E8424513A22922D0CD1DC@txhous1eb015.uson.usoncology.int><214219.31758.qm@web65715.mail.ac4.yahoo.com> <71882EED22A283429E8424513A22922D0CD1E2@txhous1eb015.uson.usoncology.int> <71882EED22A283429E8424513A22922D0CD1E3@txhous1eb015.uson.usoncology.int> Message-ID: The best tip I ever received was from a gentleman in Australia who suggested adding a very small - so small it would appear to be useless - amount of Tween 20 to the floatation water bath. The entrapment of water is eliminated - no more blebs of water at the base of the sections. Works like magic. Tresa Goins Histopathology Supervisor Department of Livestock Bozeman, Montana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Nacaela Sent: Thursday, May 12, 2011 2:25 PM To: Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nuclear bubbling Another individual called me and commented further, which clarified your statement. It is actually poor lab practice to combine the drying/baking. These steps should really be done separately so as to not "trap" the water under the tissue. I have noticed bubbles of water on the bottom of my slides that are encapsulated in wax. I assume this is also happening under the tissue as well, therefore affecting the nuclear morphology. I had been taught to speed up the process by placing them in the oven directly after cutting. Decreasing the TAT was all they really cared about. Fortunately where I work now chooses quality over quantity and I can adjust my protocol to get rid of the artifact. Thank you all for your comments and clarity. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Nacaela Sent: Thursday, May 12, 2011 2:50 PM To: Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nuclear bubbling So there really isn't a point in using an oven to dry the slides? I currently dry my slides for an hour at 55-60 degrees C. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, May 12, 2011 2:41 PM To: Johnson, Nacaela; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] nuclear bubbling Nacaela: Heat by itself is not the cause, but the heat applied to sections that have not been properly drained and still have water left between them and the slide, is. You have to drain the sections properly before heating them. Using formalin is not the cause. Ren? J. From: "Johnson, Nacaela" To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 12, 2011 12:51 PM Subject: [Histonet] nuclear bubbling Has anyone one had problems with nuclear bubbling?? I have read about two different causes.? (1) Formalin itself is known to cause the issue and (2) heating the tissue at a high temp (70 degrees C or above) while drying.? The latter is definitely not happening, but I do use formalin. I am in the process of changing the type of formalin that is used during collection.? Does anyone have any other suggestions?? Has this problem occurred for any other reason than I have already pointed out?? It seems to be worse in the surgical needle biopsies than in the bone marrow biopsies. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office:? 913-234-0576 Fax:? 913-433-7639 Email:? Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diane.Tokugawa <@t> kp.org Thu May 12 16:41:17 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Thu May 12 16:42:08 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 05/12/2011 and will not return until 05/16/2011. Note: For Cytology issues, please call Molly (day) at 8-421-5487 or Eric (eve) at 8-421-5405, For Histology issues, please call the general histology lab 8-421- 5408, Mario 8-421-4961 (day), Kiran 8-421-5404 (late afternoon/eve) or Wanda Lau for Cyto/Histo issues 8-421-5426. From CFarish <@t> csu.edu.au Thu May 12 20:35:17 2011 From: CFarish <@t> csu.edu.au (Farish, Craig) Date: Thu May 12 20:36:11 2011 Subject: [Histonet] CD79 alpha dilemma Message-ID: <3232733C82C90D439BF48C9FEF3B7D1C2C873D48AD@MAIL01.CSUMain.csu.edu.au> Hi folks - has anyone out there found a Cd79? clone and supplier which will cover multiple species reliably? I can find clones which will stain cats and dogs (and humans), and clones which will cover horses, pigs, cows and primates (and humans, but not opossums for some reason??) but i'm yet to find one which will cross-react with both the large and small animals commonly encountered in a vet lab. I would rather not have to stock 2 versions of the same antibody if I can avoid it. Any suggestions would be much appreciated. As always, thanks to everyone who contributes to histonet, Craig Craig Farish Senior Technical Officer Veterinary Diagnostic Laboratory School of Animal and Veterinary Sciences Charles Sturt University Boorooma Street Wagga Wagga NSW 2678 Australia From boor <@t> email.cz Fri May 13 02:26:33 2011 From: boor <@t> email.cz (Peter Boor) Date: Fri May 13 02:26:43 2011 Subject: [Histonet] Trilogy and Kidney Message-ID: <000101cc113f$171c8a60$45559f20$@cz> Hi everyone, We are trying to establish several stainings of GFs and their receptors (e.g. PDGF/R) in formalin fixed human kidney tissues. In our hands and according to literature a nice specific staining is seen with Trilogy Ag retrieval (using pressure cooker). However, using this Ag retrieval, independent of which primary or secondary Ab we use or how rigorous is our Av./Biot. And peroxidase blocking, we always get a strong unspecific tubular background. I was wondering if anyone encountered a similar problem and if someone has any suggestions for this? Many thanks in advance, Best regards Peter P E T E R B O O R, MD, PhD Dpt. of Nephrology & Inst. of Pathology, RWTH University Aachen Pauwelstr. 30 52074 Aachen, Germany Tel.:+49 241 80 89670 E-Mail: boor@email.cz From Nancy.F.Willis <@t> questdiagnostics.com Fri May 13 06:17:14 2011 From: Nancy.F.Willis <@t> questdiagnostics.com (Willis, Nancy F) Date: Fri May 13 06:17:23 2011 Subject: [Histonet] Ventana Kappa/Lambda Probes Message-ID: <313529606FD7D34E8AFCA589245868CB05FDE6F3@qdcws0793.us.qdx.com> We are working up the new Ventana Kappa and Lambda ISH stains. It is very weak staining on the core and the clot. The RNA positive control are very good. We are using the ImmunoCal containing formic acid for our decal solution. Does any one have it working on the core and clot. Suggestions would be appreciated. Nancy A.F. Willis, CT(ASCP) Quest Diagnostics Nichols Institute | Supervisor, Anatomic Pathology Special Testing| 14225 Newbrook Dr| Chantilly, VA USA | phone +1.703.802.6900 x65416| fax +1.703.802.7191| Nancy.f.willis@QuestDiagnostics.com | www.NicholsInstitute.com Please think about resource conservation before you print this message ------------------------------------------ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. From TGoins <@t> mt.gov Fri May 13 08:42:31 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri May 13 08:42:35 2011 Subject: [Histonet] RE: CD79 alpha dilemma In-Reply-To: <3232733C82C90D439BF48C9FEF3B7D1C2C873D48AD@MAIL01.CSUMain.csu.edu.au> References: <3232733C82C90D439BF48C9FEF3B7D1C2C873D48AD@MAIL01.CSUMain.csu.edu.au> Message-ID: We use Cd79a clone Ab-1 (HM47/A9) from Thermos Scientific (Cat #MS-357). It does not list either cats or dogs on the data sheet but it works. They also suggest HIER-Citrate pH6 for retrieval, but in our hands Citrate pH 9 produces better results - steam heat for 20 min and cool on bench top for 30 min - we an a non-automated lab. Good luck, Tresa Goins Histopathology Supervisor Department of Livestock Bozeman, Montana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Farish, Craig Sent: Thursday, May 12, 2011 7:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD79 alpha dilemma Hi folks - has anyone out there found a Cd79? clone and supplier which will cover multiple species reliably? I can find clones which will stain cats and dogs (and humans), and clones which will cover horses, pigs, cows and primates (and humans, but not opossums for some reason??) but i'm yet to find one which will cross-react with both the large and small animals commonly encountered in a vet lab. I would rather not have to stock 2 versions of the same antibody if I can avoid it. Any suggestions would be much appreciated. As always, thanks to everyone who contributes to histonet, Craig Craig Farish Senior Technical Officer Veterinary Diagnostic Laboratory School of Animal and Veterinary Sciences Charles Sturt University Boorooma Street Wagga Wagga NSW 2678 Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Fri May 13 09:14:23 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri May 13 09:14:48 2011 Subject: [Histonet] CD79 alpha dilemma References: <3232733C82C90D439BF48C9FEF3B7D1C2C873D48AD@MAIL01.CSUMain.csu.edu.au> Message-ID: Hi Craig, I have found CD79a from Biocare (cat. # CM067C) to stain dog, cat, pig, cow, horse, sheep, and mouse so far. Have not tried it on other species yet (in other words, I don't have any negative species results to report). I do have to use HIER with high pH retrieval buffer (Dako; contains EDTA) to expose antigens (in a Biocare Decloaking Chamber/pressure cooker). Citrate buffer doesn't do the job well enough. Microwave oven antigen retrieval will also work, but it exposes fewer epitopes than the pressure cooker. If you have any more questions, feel free to contact me directly. Jan Shivers Senior Scientist IHC/Histology/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "Farish, Craig" To: Sent: Thursday, May 12, 2011 8:35 PM Subject: [Histonet] CD79 alpha dilemma Hi folks - has anyone out there found a Cd79? clone and supplier which will cover multiple species reliably? I can find clones which will stain cats and dogs (and humans), and clones which will cover horses, pigs, cows and primates (and humans, but not opossums for some reason??) but i'm yet to find one which will cross-react with both the large and small animals commonly encountered in a vet lab. I would rather not have to stock 2 versions of the same antibody if I can avoid it. Any suggestions would be much appreciated. As always, thanks to everyone who contributes to histonet, Craig Craig Farish Senior Technical Officer Veterinary Diagnostic Laboratory School of Animal and Veterinary Sciences Charles Sturt University Boorooma Street Wagga Wagga NSW 2678 Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisls.com Fri May 13 11:24:16 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Fri May 13 11:24:58 2011 Subject: [Histonet] HT Position Message-ID: <6F33D8418806044682A391273399860F08205786@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! We are an 8 physician Dermatology practice located in Bucks County, PA looking for a certified HT or HTL. Candidate must meet CLIA grossing requirements. The candidate will be responsible for routine histology duties. This is a part-time position that offers a competitive salary and flexible hours. Interested applicants should contact Meredith Hale, phone (214)596-2219 or e-mail mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 214-596-7095 mhale@carisls.com From pruegg <@t> ihctech.net Fri May 13 11:59:43 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri May 13 11:59:45 2011 Subject: [Histonet] nuclear bubbling In-Reply-To: <214219.31758.qm@web65715.mail.ac4.yahoo.com> References: <71882EED22A283429E8424513A22922D0CD1DC@txhous1eb015.uson.usoncology.int> <214219.31758.qm@web65715.mail.ac4.yahoo.com> Message-ID: This is most likely caused by inadequate fixation in formalin which would protect the tissues from tissue processing. With such fast turn around times we are seeing this more and more. Put formalin on your tissue processor and use any extra time you may have up front in formalin rather than tissue processing. Ideally it takes 24 hrs to properly fix tissues so they are not damaged by processing and embedding in paraffin, but nobody fixes that long anymore. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, May 12, 2011 1:41 PM To: Johnson, Nacaela; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] nuclear bubbling Nacaela: Heat by itself is not the cause, but the heat applied to sections that have not been properly drained and still have water left between them and the slide, is. You have to drain the sections properly before heating them. Using formalin is not the cause. Ren? J. From: "Johnson, Nacaela" To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 12, 2011 12:51 PM Subject: [Histonet] nuclear bubbling Has anyone one had problems with nuclear bubbling?? I have read about two different causes.? (1) Formalin itself is known to cause the issue and (2) heating the tissue at a high temp (70 degrees C or above) while drying.? The latter is definitely not happening, but I do use formalin. I am in the process of changing the type of formalin that is used during collection.? Does anyone have any other suggestions?? Has this problem occurred for any other reason than I have already pointed out?? It seems to be worse in the surgical needle biopsies than in the bone marrow biopsies. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office:? 913-234-0576 Fax:? 913-433-7639 Email:? Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Fri May 13 12:43:30 2011 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri May 13 12:43:31 2011 Subject: [Histonet] (no subject) Message-ID: <24A4826E8EF0964D86BC5317306F58A55DF59227A3@mmc-mail.ad.mhsil.com> The C-Kit CD117 antibody from Ventana now has the following FDA approval: C-Kit has PMA FDA approval along with a companion diagnostic approval to utilize Gleevac therapy when positive for GIST tumors. Since the cost is now very high for the antibody and since it is used for predictive therapy is there another CPT code we should be charging these? Otherwise at our current price for testing we are taking a pretty good kit. How are others handling this stain? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Montina.VanMeter <@t> pbrc.edu Fri May 13 14:37:09 2011 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Fri May 13 14:38:29 2011 Subject: [Histonet] 2011 Louisiana Society for Histotechnology Annual Symposium Message-ID: Dear Histonet Colleagues, The Louisiana Society for Histotechnology would like to invite you to our annual Symposium/Conference on June 3 & 4, 2011, in Baton Rouge, LA. Meeting location: The Embassy Suites Hotel 4914 Constitution Ave. Baton Rouge, LA 70808 We have a block of rooms reserved for attendees at the special rate of $99.00. The cut-off date has been extended to May 20, 2011. After that date rooms may be reserved at the same special rate per availability. The hotel will honor this rate two days prior and two days after our meeting (please contact the Embassy Suites Hotel for further information: 1-225-924-6566 or 1-800-Embassy). A complimentary hotel shuttle service is provided for those flying into Baton Rouge. Great dining, beautiful southern plantations, and gambling boats on the Mississippi River are just a few of the attractions in the Baton Rouge area. Remember to ask for the Louisiana Society for Histotechnology group when placing your reservation. The LSH will accept credit cards through our new PayPal account. If you would like to pay via credit card, please send me your application form and the LSH will email an invoice to you that allows you to input your card information. The LSH will also accept check or cash payments. Walk-ins are always welcome! If you have any questions or would like a brochure please contact me and I will send it via email or fax: Tina Van Meter at 225-603-0953 or vanmetmj@pbrc.edu 2011 LSH Workshops: 1. MOHS Surgery and the Art of Proper Orientation in Embedding 2. In Situ and Assay Development 3. Lab Math, Team Building and Leadership within the Lab. 4. Basic IHC 5. Competency Assessment 6. "The Right Stain" 7. Multi-Systems Made Easy - (IHC) 8. Prostate Cancer and Prostate Cancer IHC Markers - Traditional & New Thank you, The LSH Education Committee From Nacaela.Johnson <@t> USONCOLOGY.COM Fri May 13 14:49:17 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Fri May 13 14:49:22 2011 Subject: [Histonet] NAACLS accredited Histotechnician program Message-ID: <71882EED22A283429E8424513A22922D0CD1ED@txhous1eb015.uson.usoncology.int> Does anyone know of an online program for HT that is NAACLS accredited? Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From Doug.Showers <@t> propath.com Fri May 13 15:11:53 2011 From: Doug.Showers <@t> propath.com (Doug Showers) Date: Fri May 13 15:11:25 2011 Subject: [Histonet] Compliments to the team Message-ID: <82C7248978CB50469FD6BA68EBBEFE6704FEF3F4@exchange.propathlab.com> Dr. Barrett sends his thanks to the Histology team for a job well done today. Doug Showers, MS, HT Histology Manager ProPath 1355 River Bend Dallas, TX 75247 214-237-1680 214-422-3083 Mobile To learn more about ProPath, please visit http://www.ProPathLab.com The information contained in this message may be privileged and confidential. If you are NOT the intended recipient, please notify the sender immediately with a copy to Postmaster@propath.com and destroy this message. From eroy <@t> illinois.edu Fri May 13 16:55:27 2011 From: eroy <@t> illinois.edu (Ed Roy) Date: Fri May 13 16:55:43 2011 Subject: [Histonet] motorized cryostat Message-ID: <4DCDA8CF.3060303@illinois.edu> I have a small research lab with an old (very old) Minot IEC microtome cryostat, which is on its last legs. In starting to shop for a new one, I was wondering how much difference a motorized one would make. We do mostly fresh frozen (unfixed) mouse brain and lymph node sections. Are the sections any better? Thanks for any opinions on this and any recommendations on a good cryostat at a reasonable cost. Ed Roy -- Edward Roy, PhD Professor of Pathology Professor of Molecular and Integrative Physiology University of Illinois at Urbana-Champaign 506 S. Mathews Ave. Urbana, IL 61801 217 333-3375 From PAGE.BALUCH <@t> asu.edu Fri May 13 18:16:32 2011 From: PAGE.BALUCH <@t> asu.edu (Debra Baluch) Date: Fri May 13 18:16:36 2011 Subject: [Histonet] Histology station for core lab Message-ID: <80BBC916BEC29A498784913D007DD4A24E3A3D28B5@EX10.asurite.ad.asu.edu> Hi All, I manage a bioimaging core facility and teach a couple of courses related to cell and tissue culture research and sample prep for live and fixed cell imaging. We have expanded to train people on the use of a cryostat, that is part of our core facility, and are now looking into obtaining a microtome to teach histological techniques. Do you have recommendations of a good rotary microtome that would work well in a core facility? Most researchers in our department manually embed and stain so we are not as familiar with the more automated instruments for sample processing. Since much of the focus is in regards to learning the process we are leaning towards going with the more manual route. We are working with a limited budget so we will need to be somewhat conservative in the models we select. Thanks for your suggestions, Page _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pvlies <@t> yahoo.com Sat May 14 02:41:44 2011 From: pvlies <@t> yahoo.com (Pam V) Date: Sat May 14 02:41:51 2011 Subject: [Histonet] (no subject) Message-ID: <927013.75116.qm@web81106.mail.mud.yahoo.com> http://www.xstreamwd.com/wp-content/themes/default/work.html From tgenade <@t> gmail.com Sat May 14 04:50:23 2011 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Sat May 14 04:50:29 2011 Subject: [Histonet] modified Gallyas stain for microglia Message-ID: Hello, I'm using the modified silver stain of Gallyas (J. Neurosci. Methods 50:159--164, 1993) for microglia. It didn't work first time round so I'm doing some trouble shooting. My main concern is the shelf life of the potassium ferroscyanide solution (1 g K3Fe(CN)6 + 0.1 mL H2SO4 per 100 mL). It is now a nice green color and looks a bit darker than yesterday. Anyone know for how long this solution is good? If anyone else has used this method I would appreciate if you could share your experience. If anyone can suggest another chemical staining protocol for microglia which works against tissues of various species I would be grateful. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From joelleweaver <@t> hotmail.com Sat May 14 06:22:27 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat May 14 06:22:31 2011 Subject: [Histonet] (no subject) Message-ID: ...I?ve got something for you! http://www.sweep.co.il/page.php?jcyhotmailID=07o5 From rjbuesa <@t> yahoo.com Sat May 14 11:49:19 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat May 14 11:49:23 2011 Subject: [Histonet] motorized cryostat In-Reply-To: <4DCDA8CF.3060303@illinois.edu> References: <4DCDA8CF.3060303@illinois.edu> Message-ID: <872151.86827.qm@web65708.mail.ac4.yahoo.com> In spite of the apparent advantage, FS are so uniquely demanding while sectioning, that the manual "touch" could never be substituted by an automatic feeding mechanism. Buy a good manual cryostat. I always used Leica. Ask for a demo. Ren? J. From: Ed Roy To: histonet@lists.utsouthwestern.edu Sent: Friday, May 13, 2011 5:55 PM Subject: [Histonet] motorized cryostat I have a small research lab with an old (very old) Minot IEC microtome cryostat, which is on its last legs. In starting to shop for a new one, I was wondering how much difference a motorized one would make.? We do mostly fresh frozen (unfixed) mouse brain and lymph node sections. Are the sections any better? Thanks for any opinions on this and any recommendations on a good cryostat at a reasonable cost. Ed Roy -- Edward Roy, PhD Professor of Pathology Professor of Molecular and Integrative Physiology University of Illinois at Urbana-Champaign 506 S. Mathews Ave. Urbana, IL 61801 217 333-3375 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat May 14 11:51:36 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat May 14 11:51:39 2011 Subject: [Histonet] Histology station for core lab In-Reply-To: <80BBC916BEC29A498784913D007DD4A24E3A3D28B5@EX10.asurite.ad.asu.edu> References: <80BBC916BEC29A498784913D007DD4A24E3A3D28B5@EX10.asurite.ad.asu.edu> Message-ID: <271397.37160.qm@web65705.mail.ac4.yahoo.com> For teaching you are right that the path is manual. For a limited budget Google for used microtomes or try eBay. Ren? J. From: Debra Baluch To: "histonet@lists.utsouthwestern.edu" Sent: Friday, May 13, 2011 7:16 PM Subject: [Histonet] Histology station for core lab Hi All, ? ? I manage a bioimaging core facility and teach a couple of courses related to cell and tissue culture research and sample prep for live and fixed cell imaging. We have expanded to train people on the use of a cryostat, that is part of our core facility, and are now looking into obtaining a microtome to teach histological techniques.? Do you have recommendations of a good rotary microtome that would work well in a core facility? Most researchers in our department manually embed and stain so we are not as familiar with the more automated instruments for sample processing. Since much of the focus is in regards to learning the process we are leaning towards going with the more manual route. We are working with a limited budget so we will need to be somewhat conservative in the models we select. Thanks for your suggestions, Page _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat May 14 14:50:45 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat May 14 14:50:48 2011 Subject: [Histonet] =?iso-8859-1?q?Re=3AHi=2E_Alice_asked_me_to_help_you_?= =?iso-8859-1?q?to_stop_smoking!_Don=92t_bother!_I_know_how_to_help_you!?= Message-ID: ...You will get all you need just sitting at home and eating popcorn! http://obermaneken.ru/page.php?iyahooID=16my7 From rsrichmond <@t> gmail.com Sat May 14 15:10:00 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat May 14 15:10:05 2011 Subject: [Histonet] Re: modified Gallyas stain for microglia Message-ID: Tyrone Genade asks: > I'm using the modified silver stain of Gallyas (J. Neurosci. Methods > 50:159--164, 1993) for microglia. It didn't work first time round so > I'm doing some trouble shooting. My main concern is the shelf life of > the potassium ferroscyanide solution (1 g K3Fe(CN)6 + 0.1 mL H2SO4 per > 100 mL). It is now a nice green color and looks a bit darker than > yesterday. Anyone know for how long this solution is good? The spelling "ferroscyanide" doesn't exactly inspire confidence - do you mean "ferricyanide"? John Kiernan did a very nice review of Gallyas stains (of which there are apparently several) on Histonet about three years ago. See http://www.histosearch.com/histonet/Apr08A/Re.HistonetGallyasStain.html John Kiernan describes these methods as quite difficult. I have no experience with them. Bob Richmond Samurai Pathologist Knoxville TN From jrsmallwood <@t> bell.net Sat May 14 16:18:36 2011 From: jrsmallwood <@t> bell.net (John Smallwood) Date: Sat May 14 16:18:50 2011 Subject: [Histonet] Outdating- Embedding Wax and Mounting Media Message-ID: To all Quality Control Purists There seems to be a growing concern with regards to outdating on reagents and their continued use beyond this date. I notice that Histological wax and Mounting media are dated with an expiry date. Just begging the question-should we go back. melt down and re-embed all old blocks in fresh wax ?? Should we recover slip old slides in fresh dated Mounting media. With all the fuss over quality control in the Laboratory and Government Lab Inspection oversight , how can this be ignored and our archives put at such dreadful risk ?? J. Smallwood,MLT. STEGH St.Thomas, Ont. Can. From contact <@t> excaliburpathology.com Sat May 14 16:55:34 2011 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Sat May 14 16:55:38 2011 Subject: [Histonet] Outdating- Embedding Wax and Mounting Media In-Reply-To: References: Message-ID: <745682.57174.qm@web1116.biz.mail.sk1.yahoo.com> How funny! You are so right!!!!! What about the?ink?on all of those old reports and reference books? Isn't ink a dye in solvent? The best special stains I ever did were back in the day when I made my own solutions from?dry chemicals that were already 20 to 30 years old. Paperwork, just a way to decrease productivity!? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: John Smallwood To: Histonet Submissions Sent: Sat, May 14, 2011 4:18:36 PM Subject: [Histonet] Outdating- Embedding Wax and Mounting Media ? To all Quality Control Purists There seems to be a growing concern with regards to outdating on reagents and their? continued use beyond this date.? I notice that Histological wax and Mounting media are dated with an expiry date. Just begging the question-should we go back. melt down and re-embed all old blocks in fresh wax ?? Should we recover slip old slides in fresh dated Mounting media. With all the fuss over quality control in the Laboratory and Government Lab Inspection oversight , how can this be ignored and our archives put at such dreadful risk ?? J. Smallwood,MLT. STEGH St.Thomas, Ont. Can. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From raestask <@t> grics.net Sat May 14 18:48:03 2011 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Sat May 14 18:48:32 2011 Subject: [Histonet] Outdating- Embedding Wax and Mounting Media In-Reply-To: <745682.57174.qm@web1116.biz.mail.sk1.yahoo.com> References: <745682.57174.qm@web1116.biz.mail.sk1.yahoo.com> Message-ID: Now you guys have done it!!! All the QAQC gurus are thinking!!! Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Saturday, May 14, 2011 4:56 PM To: John Smallwood; Histonet Subject: Re: [Histonet] Outdating- Embedding Wax and Mounting Media How funny! You are so right!!!!! What about the?ink?on all of those old reports and reference books? Isn't ink a dye in solvent? The best special stains I ever did were back in the day when I made my own solutions from?dry chemicals that were already 20 to 30 years old. Paperwork, just a way to decrease productivity!? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: John Smallwood To: Histonet Submissions Sent: Sat, May 14, 2011 4:18:36 PM Subject: [Histonet] Outdating- Embedding Wax and Mounting Media ? To all Quality Control Purists There seems to be a growing concern with regards to outdating on reagents and their? continued use beyond this date.? I notice that Histological wax and Mounting media are dated with an expiry date. Just begging the question-should we go back. melt down and re-embed all old blocks in fresh wax ?? Should we recover slip old slides in fresh dated Mounting media. With all the fuss over quality control in the Laboratory and Government Lab Inspection oversight , how can this be ignored and our archives put at such dreadful risk ?? J. Smallwood,MLT. STEGH St.Thomas, Ont. Can. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun May 15 09:41:46 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun May 15 09:41:50 2011 Subject: [Histonet] Outdating- Embedding Wax and Mounting Media In-Reply-To: References: <745682.57174.qm@web1116.biz.mail.sk1.yahoo.com> Message-ID: <745949.24832.qm@web65707.mail.ac4.yahoo.com> I think that all the chemical manufacturers (and their representatives)?that have been pushing for years this issue of "don't use beyond the shelf life" are feeling like been?"caught with their pants down". Ren? J. From: Rae Staskiewicz To: 'Paula Pierce' ; 'John Smallwood' ; 'Histonet' Sent: Saturday, May 14, 2011 7:48 PM Subject: RE: [Histonet] Outdating- Embedding Wax and Mounting Media Now you guys have done it!!! All the QAQC gurus are thinking!!! Rae Staskiewicz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Saturday, May 14, 2011 4:56 PM To: John Smallwood; Histonet Subject: Re: [Histonet] Outdating- Embedding Wax and Mounting Media How funny! You are so right!!!!! What about the?ink?on all of those old reports and reference books? Isn't ink a dye in solvent? The best special stains I ever did were back in the day when I made my own solutions from?dry chemicals that were already 20 to 30 years old. Paperwork, just a way to decrease productivity!? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: John Smallwood To: Histonet Submissions Sent: Sat, May 14, 2011 4:18:36 PM Subject: [Histonet] Outdating- Embedding Wax and Mounting Media ? To all Quality Control Purists There seems to be a growing concern with regards to outdating on reagents and their? continued use beyond this date.? I notice that Histological wax and Mounting media are dated with an expiry date. Just begging the question-should we go back. melt down and re-embed all old blocks in fresh wax ?? Should we recover slip old slides in fresh dated Mounting media. With all the fuss over quality control in the Laboratory and Government Lab Inspection oversight , how can this be ignored and our archives put at such dreadful risk ?? J. Smallwood,MLT. STEGH St.Thomas, Ont. Can. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CFarish <@t> csu.edu.au Sun May 15 19:26:37 2011 From: CFarish <@t> csu.edu.au (Farish, Craig) Date: Sun May 15 19:26:50 2011 Subject: [Histonet] RE CD79alpha dilemma - Thanks to all who replied Message-ID: <3232733C82C90D439BF48C9FEF3B7D1C2C873D4A9A@MAIL01.CSUMain.csu.edu.au> I've been given some good suggestions for both technique and clone changes. Thanks to all Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostic Laboratory School of Animal and Veterinary Sciences Charles Sturt University Boorooma Street Wagga Wagga NSW 2678 Australia From histolab <@t> pathology-partners.com Mon May 16 07:08:41 2011 From: histolab <@t> pathology-partners.com (Pathology Partners) Date: Mon May 16 07:08:44 2011 Subject: [Histonet] In need of HT/HTL in Little Rock, AR Message-ID: <373357.59356.qm@web1111.biz.mail.sk1.yahoo.com> Pathology Partners in Little Rock, Arkansas is in need?of an experienced HT or HTL to fill a full time position.? Position requirements:??Certified HT?or HTL ?????????????????????????????????? Must meet CLIA/CAP guidelines for tissue grossing ???????????????????????????????????Must?be able to demonstrate expertise in histology, including the following:? grossing GI specimens, embedding, microtomy,?IHC and special staining of?GI tissue. ? Shift: M-F 9:00am - 6:00 pm Rate of pay is hourly and negotiable based on experience. Full benefits including vision, dental and health available. 90 day probationary period. Please?fax all resumes to 501-687-9228 or email to histolab@pathology-partners.com Call 501-687-9220 and ask for Clay. Sincerely, Clay Milks? Histology Laboratory Pathology Partners LLC histolab@pathology-partners.com ? Confidentiality Notice: This e-mail message, including any attachments,is for the sole use of the intended recipient(s) and may contain confidential and privileged information.? Any unauthorized review,use, disclosure or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From dmccaig <@t> ckha.on.ca Mon May 16 07:46:34 2011 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Mon May 16 07:46:46 2011 Subject: [Histonet] uric acid crystals in tissue for gout Message-ID: Does anyone have a method for identifying uric acid crystals in gout on a tissue sample. Diana From mw <@t> personifysearch.com Mon May 16 07:58:01 2011 From: mw <@t> personifysearch.com (Matt Ward) Date: Mon May 16 07:58:09 2011 Subject: [Histonet] Field Support Opportunity with a World Leader Message-ID: Good Morning Histonet! One of our retained Cancer Diagnostics clients has opened up an expansion Field Technical Support Opportunity perfect for a Histotech looking to get out of the lab and into the field. Please send your resumes to mw@personifysearch.com, or call me at 800.875.6188 ext. 103 so I can answer any questions. Also, if you know of anyone who may be interested please do not hesitate to forward this e-mail, and have them reach out to me. Have a great day! Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com *Field Support Specialist* *The Company:* Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. *The Opportunity:* The company currently has an opening for a Field Support Specialist in Cancer Diagnostics to cover the Mid Atlantic Region and to be based in Baltimore or Washington DC. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary. Base: $60-65k per year + bonus/commission Car Allowance: $700/month + Gas Card Cell Phone/Laptop Other: Full benefits - 401k program/matching *Primary Responsibilities: * The primary responsibility of this role will be to provide in-field technical applications support for current and next generation range of products. The FSS will install/set-up instrumentation in customer laboratories, perform demonstrations and maintain demonstration equipment in a clean and operational manner. The FSS will also train customers on the use of instrumentation. Conducting in-field post purchase applications support and performing instrument validations will be a key responsibility of this position. Additional Responsibilities: - Build an in-depth understanding of new product technologies and their applications in a diagnostic environment - Contribute to the strategic planning process by providing technical data on instrumentation and reagents - Conduct post-purchase reagent order management - Be the customer liaison by providing scientific and laboratory expertise for in-field installations, evaluations, training and trouble-shooting - Provide customer support for remote problem solving - Design and perform experiments to investigate and solve tough technical applications problems From lbustamante <@t> cvm.tamu.edu Mon May 16 09:30:21 2011 From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante) Date: Mon May 16 09:30:28 2011 Subject: [Histonet] Microtome Quote Message-ID: <4DD0EEAD020000B9001012FE@CVM.TAMU.EDU> I am so sorry I misplaced the hard copy I made of a quote I need and my computer crashed with a storm we had last week. Someone sent me a quote for 4 Leica/Reichert 2030 rotary microtomes and 2 or 4 water bath. The price for each microtome was $3,200 (I believe) and the water bath less than $700.00 each. Please send me the quote again. Thank you and I apologise again. Lin Bustamante Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 From foreightl <@t> gmail.com Mon May 16 10:21:05 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Mon May 16 10:21:08 2011 Subject: [Histonet] uric acid crystals in tissue for gout In-Reply-To: References: Message-ID: Here we just process starting in 70% EtOH, cut the section on a bath of 70% EtOH (watch out, it wrinkles very bad) and stain them in an Eosin only stain (which is the H&E with all of the aqueous parts taken out). The pathologist looks at them with a polarizing filter. Make sure that they stay away from water! A good article that talks about this is: Evaluation of Crystals in Formalin-Fixed, Paraffin-Embedded Tissue Sections for the Differential Diagnosis of Pseudogout, Gout, and Tumoral Calcinosis Shidham et. al. Mod Pathol 2001;14(8):806?810 Good luck! On Mon, May 16, 2011 at 5:46 AM, Diana McCaig wrote: > Does anyone have a method for identifying uric acid crystals in gout on > a tissue sample. > > > > Diana > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From Doug.Showers <@t> propath.com Mon May 16 12:12:07 2011 From: Doug.Showers <@t> propath.com (Doug Showers) Date: Mon May 16 12:12:07 2011 Subject: [Histonet] Job Opening in Dallas, Texas Message-ID: <82C7248978CB50469FD6BA68EBBEFE6704FEF3FE@exchange.propathlab.com> ProPath, a progressive, CAP accredited, high volume, pathology practice, located in Dallas, Texas, is seeking a Histotechnologist. The hours for this position are Monday through Friday, 2:00 pm to 10:30 pm. In this position you will be responsible for embedding tissue specimens, microtomy of paraffin-embedded tissue, operation of automated stainers and coverslippers, equipment maintenance and record retention. The ideal candidate will have a high school diploma or equivalent. We prefer, HT, HTL (ASCP) registered or eligible. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! EOE For consideration send resume to: ProPath, Human Resources 1355 River Bend Drive Dallas, TX 75247 FAX: 214/237-1825 Job-Line: 214/237-1775 Email address: jobs@propath.com Website: www.propath.com Doug Showers, MS, HT Histology Manager ProPath 1355 River Bend Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com The information contained in this message may be privileged and confidential. If you are NOT the intended recipient, please notify the sender immediately with a copy to Postmaster@propath.com and destroy this message. From rjr6 <@t> psu.edu Mon May 16 12:12:50 2011 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Mon May 16 12:13:00 2011 Subject: [Histonet] Amyloid stain Message-ID: Does anyone have a procedure to do an amyloid stain on a smear? Someone at the university wants to see if there is amyloid in milk. Roberta Horner HT/HTl Animal Diagnostic Lab Penn State University From rsrichmond <@t> gmail.com Mon May 16 12:13:35 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon May 16 12:13:44 2011 Subject: [Histonet] Re: uric acid crystals in tissue for gout Message-ID: To see uric acid (actually monosodium urate) crystals in tissue, the best thing to do is pick them out of the wet tissue with a needle, put them on a slide in a drop of water with a coverslip, and examine them promptly with polarization. If they can't be seen on gross examination, then the special processing techniques mentioned certainly help, though often MSU will come through in routine processing. The real problem is for the pathologist. Most of the small-hospital pathologists I work with aren't allowed to have a polarizer on their microscopes, so they have to take the trouble to go to the urine scope (no, the broken-sunglasses shtick doesn't work), if they're allowed in the urine lab which they sometimes aren't. And if they're willing to take the time. To identify MSU, you need to demonstrate negative birefringence. That requires a full wave plate system (sometimes called a first order plate), and somebody has to have preserved the documentation so that the microscopist knows which way is up. In the absence of a procedure manual, the pee scope can be quite frustrating. (Also I have to spend five minutes cleaning and aligning it.) I'm not telling you anything I don't have to put up with at least once a month. Bob Richmond Samurai Pathologist Knoxville TN From HParker <@t> Skaggs.Net Mon May 16 12:24:28 2011 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Mon May 16 12:25:58 2011 Subject: [Histonet] Dictaphone System - HELP !! In-Reply-To: References: Message-ID: <930EB2E8DF68C544873EDD2A3D5F5060048DC35A66@email1.skaggs.net> Hi All, Does anybody use the old Dictaphones ? We have been using them and the company does not support them any longer. The one at our grossing station went down Friday and was replaced by a newer one (as we had purchased several from a hospital that was getting out of them). This one did not have a foot pedal so a foot pedal connection was adapted to it; however, when the other machine went down it was unplugged by another team member and all the programming was therefore lost. Does anyone have Dictaphones that are operated by means of a foot pedal who's IT team would be willing to share the proper method to program in a foot pedal to use w/ it. It is almost impossible to gross without a foot pedal. Thanks in advance. Sincerely, Helayne Parker, HT (ASCP) CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. From kblack <@t> digestivehlth.com Mon May 16 12:42:13 2011 From: kblack <@t> digestivehlth.com (Konni Black) Date: Mon May 16 12:42:20 2011 Subject: [Histonet] HT/ HTL Position Columbia, SC Message-ID: <4F673614A9714DF79543ED8E3785717A@digestivehlth.com> Hi All, There is a fulltime opening for an experienced HT or HTL in Columbia, SC. Construction of this beautiful new lab will be completed by July. The ideal candidate will have 3 to 5 years experience and be able to work independently. They offer great pay and benefits and a very flexible schedule....no need to work at 4AM unless that is what YOU want! Interested parties may call @ 253-503-2560 or e-mail me directly kblack@digestivehlth.com. Thank you, Konni Black From laurie.colbert <@t> huntingtonhospital.com Mon May 16 13:06:51 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon May 16 13:06:53 2011 Subject: [Histonet] uric acid crystals in tissue for gout In-Reply-To: References: Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AC67@EXCHANGE3.huntingtonhospital.com> We receive these specimens fresh and hand-process through 100% alcohol, xylene, and paraffin. The crystals are water soluble, so you need to avoid any aqueous solutions. We cut one slide for routine H&E staining and one slide that we just deparaffinize in xylene and then coverslip directly out of that xylene. The pathologist looks at the unstained slide with a polarizing lens. If the tissue gets processed routinely (through formalin and the graded alcohols), we still cut the two slides and sometimes the crystals are still there. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Monday, May 16, 2011 5:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] uric acid crystals in tissue for gout Does anyone have a method for identifying uric acid crystals in gout on a tissue sample. Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robin_dean <@t> compbio.com Mon May 16 13:07:02 2011 From: robin_dean <@t> compbio.com (Robin Dean) Date: Mon May 16 13:10:20 2011 Subject: [Histonet] sections falling off slides Message-ID: <003601cc13f4$0f0908a0$2d1b19e0$@com> Hi All, We are trying to do IHC for multiple antigens on goat knees. The sections are decalcified FFPE sections and we are using a Dako autostainer. We are currently trying air-drying for 1-2 days and baking of tissue slides before use in IHC.. The one previous time we tried baking, we noticed a decrease in IHC signal. The sections are pretty large, we tried cutting them down, but can't decrease the size much. We also decreased the number of washes and size of washes on the Daok, which helped only slightly. Does anyone have any suggestions? Thank you in advance. Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com From liz <@t> premierlab.com Mon May 16 13:19:49 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon May 16 13:19:53 2011 Subject: [Histonet] sections falling off slides In-Reply-To: <003601cc13f4$0f0908a0$2d1b19e0$@com> Message-ID: Robin We run quite a few IHC's on goat bone femurs. We use a very good plus slide. Dry flat at 40C overnight and let them sit a few days before we stain, we do not place them in a 60C oven since we find that the articular cartilage may flip. We have good success with proteinase K with staining on the dako stainer for several antibodies. If we have to run HIER we use a 70C retrieval for 2 hours and then may choose to manually stain depending upon the samples rather then placing on the dako autostainer. We have done this type of staining on porcine femurs on 2x3 slides manually. I really think tissue adherence is dependent upon good fixation, optimal decalcification and proper processing, if samples have been insufficiently decaled or processed you are going to run into problems with tissue adherence. I can't comment to the lack of staining intensity we always pilot a few slides prior to staining the entire study just to make sure our methods will work for the particular tissues we are currently working on. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robin Dean Sent: Monday, May 16, 2011 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sections falling off slides Hi All, We are trying to do IHC for multiple antigens on goat knees. The sections are decalcified FFPE sections and we are using a Dako autostainer. We are currently trying air-drying for 1-2 days and baking of tissue slides before use in IHC.. The one previous time we tried baking, we noticed a decrease in IHC signal. The sections are pretty large, we tried cutting them down, but can't decrease the size much. We also decreased the number of washes and size of washes on the Daok, which helped only slightly. Does anyone have any suggestions? Thank you in advance. Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Mon May 16 13:21:45 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon May 16 13:24:11 2011 Subject: [Histonet] RE: Dictaphone System - HELP !! In-Reply-To: <930EB2E8DF68C544873EDD2A3D5F5060048DC35A66@email1.skaggs.net> References: <930EB2E8DF68C544873EDD2A3D5F5060048DC35A66@email1.skaggs.net> Message-ID: <3AD061FE740D464FAC7BF6B5CFB757070104ED@SMCMAIL01.somerset-healthcare.com> We use the old Dicatphones too. Our service is provided from Nuance Communications. You can reach them at 866-383-9031. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Monday, May 16, 2011 1:24 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Dictaphone System - HELP !! Hi All, Does anybody use the old Dictaphones ? We have been using them and the company does not support them any longer. The one at our grossing station went down Friday and was replaced by a newer one (as we had purchased several from a hospital that was getting out of them). This one did not have a foot pedal so a foot pedal connection was adapted to it; however, when the other machine went down it was unplugged by another team member and all the programming was therefore lost. Does anyone have Dictaphones that are operated by means of a foot pedal who's IT team would be willing to share the proper method to program in a foot pedal to use w/ it. It is almost impossible to gross without a foot pedal. Thanks in advance. Sincerely, Helayne Parker, HT (ASCP) CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From nelsonrnch <@t> verizon.net Mon May 16 13:36:42 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Mon May 16 13:36:44 2011 Subject: [Histonet] Full Time Position in Long Beach Calif. Message-ID: <37271.39996.qm@web84303.mail.re1.yahoo.com> Hi All HT/HTL Gastroenterology Group opening New Histology Lab in Long Beach Calif. Lab to be completed approximately in August. Candidate to have 3-5 years experience, self motivated and able to work well independently. Flexible hours, great pay. For more detailed information please contact me at information below. THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net From nelsonrnch <@t> verizon.net Mon May 16 13:36:42 2011 From: nelsonrnch <@t> verizon.net (SHANE NELSON) Date: Mon May 16 13:36:45 2011 Subject: [Histonet] Full Time Position in Long Beach Calif. Message-ID: <37271.39996.qm@web84303.mail.re1.yahoo.com> Hi All HT/HTL Gastroenterology Group opening New Histology Lab in Long Beach Calif. Lab to be completed approximately in August. Candidate to have 3-5 years experience, self motivated and able to work well independently. Flexible hours, great pay. For more detailed information please contact me at information below. THANK YOU, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANT SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net From HParker <@t> Skaggs.Net Mon May 16 14:46:25 2011 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Mon May 16 14:47:42 2011 Subject: [Histonet] Dictaphone Troubles are over In-Reply-To: <1efadc2e-c5cd-409b-bb62-824e0ff6466f@email1.skaggs.net> References: <1efadc2e-c5cd-409b-bb62-824e0ff6466f@email1.skaggs.net> Message-ID: <930EB2E8DF68C544873EDD2A3D5F5060048DC35A6A@email1.skaggs.net> Thanks all- we got it fixed and it works like new now. Thanks, Helayne Parker, H.T. (ASCP) Pathology Section Head Skaggs Regional Medical Center The Best Place to Get Better P.O. Box 650, Branson Missouri 65615 Direct: 417-335-7254 Fax: 417-335-7127 E-Mail: hparker@skaggs.net Web: www.skaggs.net CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. From rjbuesa <@t> yahoo.com Mon May 16 15:23:02 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 16 15:23:05 2011 Subject: [Histonet] sections falling off slides In-Reply-To: <003601cc13f4$0f0908a0$2d1b19e0$@com> References: <003601cc13f4$0f0908a0$2d1b19e0$@com> Message-ID: <863799.66085.qm@web65703.mail.ac4.yahoo.com> Your test subject is one of the more difficult there are. Although you may get some background, use some gelatin dissolved in the water bath to try to adhere the sections to the slides. Also try to cut as thin as you can and make sure there is no water left between the sections and the slide. Bake at 60?C for at least 45 minutes before starting the IHC procedure. If you use HIER try to switch to pepsin. The least "stress" you add to the sections the better. Ren? J. From: Robin Dean To: histonet@lists.utsouthwestern.edu Sent: Monday, May 16, 2011 2:07 PM Subject: [Histonet] sections falling off slides Hi All, We are trying to do IHC for multiple antigens on goat knees. The sections are decalcified FFPE sections and we are using a Dako autostainer. We are currently trying air-drying for? 1-2 days? and baking of tissue slides before use in IHC.. The one previous time we tried baking, we noticed a decrease in IHC signal. The sections are pretty large, we tried cutting them down, but can't decrease the size much.? We also? decreased the number of washes and size of washes on the Daok, which helped only slightly.? Does anyone have any suggestions? Thank you in advance. Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosie_scrimizzi <@t> hotmail.com Mon May 16 17:48:25 2011 From: rosie_scrimizzi <@t> hotmail.com (Rosie Scrimizzi) Date: Mon May 16 17:48:30 2011 Subject: [Histonet] uric acid crystals in tissue for gout In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2AC67@EXCHANGE3.huntingtonhospital.com> References: , <57BE698966D5C54EAE8612E8941D76830AD2AC67@EXCHANGE3.huntingtonhospital.com> Message-ID: You can also do dab smears with the fresh tissue on a slide, pop a coverslip on top and examine the wet prep under a polarising microscope. I agree routine processing doesn't completely remove UA crystals and an unstained section as described below can be used to demonstrate UA Rosie Scrimizzi Siemens Australia > Date: Mon, 16 May 2011 11:06:51 -0700 > From: laurie.colbert@huntingtonhospital.com > To: dmccaig@ckha.on.ca; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] uric acid crystals in tissue for gout > CC: > > We receive these specimens fresh and hand-process through 100% alcohol, > xylene, and paraffin. The crystals are water soluble, so you need to > avoid any aqueous solutions. We cut one slide for routine H&E staining > and one slide that we just deparaffinize in xylene and then coverslip > directly out of that xylene. The pathologist looks at the unstained > slide with a polarizing lens. If the tissue gets processed routinely > (through formalin and the graded alcohols), we still cut the two slides > and sometimes the crystals are still there. > Laurie Colbert > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana > McCaig > Sent: Monday, May 16, 2011 5:47 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] uric acid crystals in tissue for gout > > Does anyone have a method for identifying uric acid crystals in gout on > a tissue sample. > > > > Diana > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmweber113 <@t> comcast.net Mon May 16 19:32:10 2011 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Mon May 16 19:32:13 2011 Subject: [Histonet] chemical waste co Message-ID: <358641395.331503.1305592330609.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Hello,? Does anyone have a name of a laboratory chemical waste company in Southern California? Thanks for your help. Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 From shive003 <@t> umn.edu Tue May 17 08:48:45 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue May 17 08:48:50 2011 Subject: Fw: [Histonet] CD79 alpha dilemma - ANSWER CORRECTION Message-ID: Hello everyone - my apologies for posting a partially incorrect answer last week. I was looking at the wrong line on a spreadsheet for CD79a abbreviated protocol information and wrote down the wrong HIER information. What we do here currently with HIER for Biocare's CD79a (clone HM47/A9) on dogs, cats, pigs, cows, etc., is unmask antigens using: 0.1M Citrate buffer, pH 6.0 in a Biocare Decloaking Chamber. (High pH retrieval solution does expose more antigenic sites, but it damages the tissue more when using the pressure cooker method.) In the past we used high pH retrieval solution when doing HIER using a microwave oven (gentler method). Jan Shivers UMN Vet Diag Lab ----- Original Message ----- From: "Jan Shivers" To: "Farish, Craig" ; Sent: Friday, May 13, 2011 9:14 AM Subject: Re: [Histonet] CD79 alpha dilemma > Hi Craig, > > I have found CD79a from Biocare (cat. # CM067C) to stain dog, cat, pig, cow, > horse, sheep, and mouse so far. Have not tried it on other species yet (in > other words, I don't have any negative species results to report). > > I do have to use HIER with high pH retrieval buffer (Dako; contains EDTA) to > expose antigens (in a Biocare Decloaking Chamber/pressure cooker). Citrate > buffer doesn't do the job well enough. Microwave oven antigen retrieval > will also work, but it exposes fewer epitopes than the pressure cooker. > > If you have any more questions, feel free to contact me directly. > > Jan Shivers > Senior Scientist > IHC/Histology/EM Section Head > Pathology Teaching Program > University of Minnesota > Veterinary Diagnostic Laboratory > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > > (Confidentiality Notice: This message, together with any attachments, is > intended only for the use of the individual or entity to which it is > addressed and may contain confidential or privileged information. If you > think you have received this message in error, please advise the sender and > then delete this message and any attachments immediately.) > > > > ----- Original Message ----- > From: "Farish, Craig" > To: > Sent: Thursday, May 12, 2011 8:35 PM > Subject: [Histonet] CD79 alpha dilemma > > > Hi folks - has anyone out there found a Cd79? clone and supplier which will > cover multiple species reliably? > I can find clones which will stain cats and dogs (and humans), and clones > which will cover horses, pigs, cows and primates (and humans, but not > opossums for some reason??) but i'm yet to find one which will cross-react > with both the large and small animals commonly encountered in a vet lab. I > would rather not have to stock 2 versions of the same antibody if I can > avoid it. > Any suggestions would be much appreciated. > As always, thanks to everyone who contributes to histonet, > Craig > > Craig Farish > Senior Technical Officer > Veterinary Diagnostic Laboratory > School of Animal and Veterinary Sciences > Charles Sturt University > Boorooma Street > Wagga Wagga > NSW 2678 > Australia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dreynold <@t> mdanderson.org Tue May 17 08:54:20 2011 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Tue May 17 08:54:23 2011 Subject: [Histonet] motorized cryostat In-Reply-To: <2eabaa87-50e4-431e-b958-f10886bbceae@DCPWPRTR01.mdanderson.edu> References: <2eabaa87-50e4-431e-b958-f10886bbceae@DCPWPRTR01.mdanderson.edu> Message-ID: <785BBF0C5F49CE41BA74460A43A08F022E2E1B86D1@DCPWVMBXC0VS3.mdanderson.edu> We have a motorized and rarely use the motorized function, Maybe 5 time in the last 5 years. It is faster to cut manually. Donna Reynolds, chief Histology research lab U.T. M.D. Anderson Cancer Center, Houston, TX 713-792-8106 From laurie.colbert <@t> huntingtonhospital.com Tue May 17 09:00:31 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue May 17 09:00:37 2011 Subject: [Histonet] chemical waste co In-Reply-To: <358641395.331503.1305592330609.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> References: <358641395.331503.1305592330609.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AC72@EXCHANGE3.huntingtonhospital.com> We work with Stericycle, and they are great! I've worked with them for years. They pick up all of our chemical and biohazard waste and also separate the formalin from the specimens for disposal. Their number is (323) 266-6448. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rmweber113@comcast.net Sent: Monday, May 16, 2011 5:32 PM To: histonet Subject: [Histonet] chemical waste co Hello,? Does anyone have a name of a laboratory chemical waste company in Southern California? Thanks for your help. Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sara.Lees <@t> covance.com Tue May 17 09:17:37 2011 From: Sara.Lees <@t> covance.com (Lees, Sara) Date: Tue May 17 09:17:48 2011 Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Message-ID: <248907F494F5B740A86B9C6FF4C3D59F5F12CC@crwxch04.ent.covance.com> Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it, u name it and nothing has worked...Can anyone help Thanks Sara ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From JWeems <@t> sjha.org Tue May 17 09:29:02 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue May 17 09:29:07 2011 Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems In-Reply-To: <248907F494F5B740A86B9C6FF4C3D59F5F12CC@crwxch04.ent.covance.com> References: <248907F494F5B740A86B9C6FF4C3D59F5F12CC@crwxch04.ent.covance.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081F5A13EA@CHEXCMS10.one.ads.che.org> Make sure the needle is clean - soak every night. And be sure your coverslips are clean. Look closely - there may be ground glass on the coverslips.. Then kiss it some more...:>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lees, Sara Sent: Tuesday, May 17, 2011 10:18 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it, u name it and nothing has worked...Can anyone help Thanks Sara ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From flnails <@t> texaschildrens.org Tue May 17 09:33:40 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue May 17 09:33:50 2011 Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems In-Reply-To: <248907F494F5B740A86B9C6FF4C3D59F5F12CC@crwxch04.ent.covance.com> References: <248907F494F5B740A86B9C6FF4C3D59F5F12CC@crwxch04.ent.covance.com> Message-ID: If you are using one of the older film coverslippers there is a roller following the application of the film. Make sure that roller is still in the correct place or that one of the springs is not missing or in need of replacing. The job of that roller is to squeeze the air bubbles from under the film. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lees, Sara Sent: Tuesday, May 17, 2011 9:18 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it, u name it and nothing has worked...Can anyone help Thanks Sara ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From contact <@t> excaliburpathology.com Tue May 17 09:36:30 2011 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue May 17 09:36:36 2011 Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems In-Reply-To: <248907F494F5B740A86B9C6FF4C3D59F5F12CC@crwxch04.ent.covance.com> References: <248907F494F5B740A86B9C6FF4C3D59F5F12CC@crwxch04.ent.covance.com> Message-ID: <461875.69588.qm@web1108.biz.mail.sk1.yahoo.com> Thin the mounting media with?xylene or solvent you use. I use Permount thinned 60/40 with xylene. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Lees, Sara" To: "histonet@lists.utsouthwestern.edu" Sent: Tue, May 17, 2011 9:17:37 AM Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it,? u name it and nothing has worked...Can anyone help Thanks Sara ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From af46 <@t> buffalo.edu Tue May 17 09:38:49 2011 From: af46 <@t> buffalo.edu (Annette Featherstone) Date: Tue May 17 09:38:54 2011 Subject: [Histonet] Freezing rodent brain Message-ID: How is everyone freezing hippocampus sections? The current method being used seems to be causing a lot of artifact. Thanks Annette Featherstone From Charles.Scouten <@t> leica-microsystems.com Tue May 17 09:42:10 2011 From: Charles.Scouten <@t> leica-microsystems.com (Charles.Scouten@leica-microsystems.com) Date: Tue May 17 09:42:52 2011 Subject: [Histonet] Freezing rodent brain Message-ID: It must be completed in seconds. Buried in powdered dry ice is one way. Immersion in a slurry of dry ice and isopentane is another. Cordially, Charles W. Scouten, Ph.D Product Specialist, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annette Featherstone Sent: Tuesday, May 17, 2011 9:39 AM To: Subject: [Histonet] Freezing rodent brain How is everyone freezing hippocampus sections? The current method being used seems to be causing a lot of artifact. Thanks Annette Featherstone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From rjbuesa <@t> yahoo.com Tue May 17 09:43:24 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 17 09:43:28 2011 Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems In-Reply-To: <248907F494F5B740A86B9C6FF4C3D59F5F12CC@crwxch04.ent.covance.com> References: <248907F494F5B740A86B9C6FF4C3D59F5F12CC@crwxch04.ent.covance.com> Message-ID: <457460.66374.qm@web65708.mail.ac4.yahoo.com> Are you using the original Sakura film? Ren? J. From: "Lees, Sara" To: "'histonet@lists.utsouthwestern.edu'" Sent: Tuesday, May 17, 2011 10:17 AM Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it,? u name it and nothing has worked...Can anyone help Thanks Sara ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jasonlyoder <@t> gmail.com Tue May 17 09:48:09 2011 From: jasonlyoder <@t> gmail.com (Jason Yoder) Date: Tue May 17 09:48:13 2011 Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems In-Reply-To: <248907F494F5B740A86B9C6FF4C3D59F5F12CC@crwxch04.ent.covance.com> References: <248907F494F5B740A86B9C6FF4C3D59F5F12CC@crwxch04.ent.covance.com> Message-ID: Sara, The same problem with air bubbles developed for me as well. I hadn't changed anything but it slowly got worse. On a hunch I changed the xylene substitute and it corrected everything. Can't say that I know why, but I my guess was that the miscibility with the mounting media was becoming worse. The shelf life was still good on the mounting media but it had been in stock for some time. I don't know if it makes any difference but I cover slipping manually. Of course, it was our first guess that someone was being inattentive while cover slipping but it became clear that that wasn't the case. good luck, Jason On Tue, May 17, 2011 at 7:17 AM, Lees, Sara wrote: > Hi All, > > I am having horrible problems with air bubbles , can anyone help I have > changed the speed increased the mountant volume, clean it, kissed it, u > name it and nothing has worked...Can anyone help > > > Thanks Sara > > > > > ----------------------------------------------------- > Confidentiality Notice: This e-mail transmission > may contain confidential or legally privileged > information that is intended only for the individual > or entity named in the e-mail address. If you are not > the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or reliance > upon the contents of this e-mail is strictly prohibited. > > If you have received this e-mail transmission in error, > please reply to the sender, so that we can arrange > for proper delivery, and then please delete the message > from your inbox. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From kreaser <@t> vet.upenn.edu Tue May 17 09:51:14 2011 From: kreaser <@t> vet.upenn.edu (Karie Reaser) Date: Tue May 17 09:51:17 2011 Subject: [Histonet] Filter Paper for Harris Hematoxylin Message-ID: <57297645.8518918.1305643874162.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> Good morning, What grade filter paper do you use for purchased Harris Hematoxylin filtering? I have used 3 different grades, coarse and still have precipitate on my slides. Any advice is greatly appreciated. Thank you Karie -- Karie L Reaser A.S. New Bolton Center University of Pennsylvania School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 382 W Street Road Kennett Square, PA. 19348 Phone:610-925-6278 Fax:610-925-6820 Email:kreaser@vet.upenn.edu From Erin.Bertani <@t> RoswellPark.org Tue May 17 09:54:15 2011 From: Erin.Bertani <@t> RoswellPark.org (Bertani, Erin) Date: Tue May 17 09:54:20 2011 Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems In-Reply-To: <461875.69588.qm@web1108.biz.mail.sk1.yahoo.com> References: <248907F494F5B740A86B9C6FF4C3D59F5F12CC@crwxch04.ent.covance.com> <461875.69588.qm@web1108.biz.mail.sk1.yahoo.com> Message-ID: Try an extended prime when you start it in the am, it seemed to resolve the issue with our glass coverslipper at least for now. (We were having a horrible time with it, and that's what the repair man did. No bubbles for five days and running!) Erin G. Bertani, B.A. Clinical Laboratory Assistant Department of Pathology and Laboratory Medicine Roswell Park Cancer Institute Elm & Carlton Sts Buffalo, NY 14263 Phone: 716-845-3145 Fax: 716-845-2370 erin.bertani@roswellpark.org ? -----Original Message----- From: Paula Pierce [mailto:contact@excaliburpathology.com] Sent: Tuesday, May 17, 2011 10:37 AM To: Lees, Sara; Histonet Subject: Re: [Histonet] Sakura Tissue-tek Glas Air bubble problems Thin the mounting media with?xylene or solvent you use. I use Permount thinned 60/40 with xylene. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Lees, Sara" To: "histonet@lists.utsouthwestern.edu" Sent: Tue, May 17, 2011 9:17:37 AM Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it,? u name it and nothing has worked...Can anyone help Thanks Sara ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From Erin.Bertani <@t> RoswellPark.org Tue May 17 09:55:47 2011 From: Erin.Bertani <@t> RoswellPark.org (Bertani, Erin) Date: Tue May 17 09:55:51 2011 Subject: FW: [Histonet] Sakura Tissue-tek Glas Air bubble problems Message-ID: Try an extended prime when you start it in the am, it seemed to resolve the issue with our glass coverslipper at least for now. (We were having a horrible time with it, and that's what the repair man did. No bubbles for five days and running!) Erin G. Bertani, B.A. Clinical Laboratory Assistant Department of Pathology and Laboratory Medicine Roswell Park Cancer Institute Elm & Carlton Sts Buffalo, NY 14263 Phone: 716-845-3145 Fax: 716-845-2370 erin.bertani@roswellpark.org ? -----Original Message----- From: Paula Pierce [mailto:contact@excaliburpathology.com] Sent: Tuesday, May 17, 2011 10:37 AM To: Lees, Sara; Histonet Subject: Re: [Histonet] Sakura Tissue-tek Glas Air bubble problems Thin the mounting media with?xylene or solvent you use. I use Permount thinned 60/40 with xylene. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Lees, Sara" To: "histonet@lists.utsouthwestern.edu" Sent: Tue, May 17, 2011 9:17:37 AM Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it,? u name it and nothing has worked...Can anyone help Thanks Sara ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From alyssa <@t> alliedsearchpartners.com Tue May 17 10:01:13 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa) Date: Tue May 17 10:01:18 2011 Subject: [Histonet] Histotech Job Opening in Fort Myers, FL 3rd Shift Message-ID: Allied Search Partners is currently looking for a qualified applicant for a Histotechnologist or Histotechnician willing to work the third shift within a Fort Myers, FL laboratory. Position: Histotechnologist or Histotechnician Schedule: Monday-Friday, Overnight hours/Third Shift Summary: Perform a variety of routine and specialized histology techniques and procedures. Embedding, Microtomy, Grossing, Processing, and H&E staining Special Staining Equipment maintenance Requirements: FL Laboratory License Previous experience with automated IHC Technical and QC protocols AA or BS/BA degree in life science preferred but not required Benefits: Competitive salaries, Health, Dental, Life & Disability insurances, a section 125 plan, a 401K, FSA, ESPP and relocation assistance. To Apply: Please send resume to alyssa@alliedsearchpartners.com Alyssa Peterson, Candidate Recruitment LinkedIn: http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From algranth <@t> email.arizona.edu Tue May 17 10:13:25 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue May 17 10:13:37 2011 Subject: [Histonet] Kim Tournear Message-ID: <3D2D7DBC-DE98-4396-BFEB-0C90CB85C22B@email.arizona.edu> Need to speak to Kim Tournear and I don't have her contact info. Kim, if you are reading this - call me. 520.626.4415 Andi Grantham From ArtimK <@t> slhn.org Tue May 17 10:57:29 2011 From: ArtimK <@t> slhn.org (Artim, Kimberly) Date: Tue May 17 10:57:35 2011 Subject: [Histonet] Paraffin temps Message-ID: <9E67FDD215B226448638018A82B952BD021E7261D56A@EXCHANGE.slhn.org> Could someone please share with me their standard for setting an acceptable temperature range for the paraffin stations on the processors? Kimberly Artim, AST, HT (ASCP) Technical Coordinator, Anatomic Pathology St Lukes Hospital & Health Network 801 Ostrum Street Bethlehem, PA 18015 610-954-4832 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Tue May 17 11:16:32 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 17 11:16:35 2011 Subject: [Histonet] Paraffin temps In-Reply-To: <9E67FDD215B226448638018A82B952BD021E7261D56A@EXCHANGE.slhn.org> References: <9E67FDD215B226448638018A82B952BD021E7261D56A@EXCHANGE.slhn.org> Message-ID: <606378.69928.qm@web65716.mail.ac4.yahoo.com> Each paraffin bag states the melting point temperature of the contained paraffin. Just use that value +1?C Ren? J. From: "Artim, Kimberly" To: "'histonet@lists.utsouthwestern.edu'" Sent: Tuesday, May 17, 2011 11:57 AM Subject: [Histonet] Paraffin temps Could someone please share with me their standard for setting an acceptable temperature range for the paraffin stations on the processors? Kimberly Artim, AST, HT (ASCP) Technical Coordinator, Anatomic Pathology St Lukes Hospital & Health Network 801 Ostrum Street Bethlehem, PA 18015 610-954-4832 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information.? Any unauthorized review, use, disclosure or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aj.taylor <@t> blueyonder.co.uk Tue May 17 11:40:06 2011 From: aj.taylor <@t> blueyonder.co.uk (taylor alan) Date: Tue May 17 11:40:11 2011 Subject: [Histonet] Filter Paper for Harris Hematoxylin In-Reply-To: <57297645.8518918.1305643874162.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> References: <57297645.8518918.1305643874162.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> Message-ID: Hi Karie Whatman No: 1 Qualitative Grade should effectively remove all precipitate and leave you with a fresh clean staining solution. Alan Taylor Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter Devon. EX1 3AJ Tel: +44(0)1392) 660132 On 17 May 2011 15:51, Karie Reaser wrote: > > Good morning, > What grade filter paper do you use for purchased Harris Hematoxylin > filtering? I have used 3 different grades, coarse and still have precipitate > on my slides. Any advice is greatly appreciated. Thank you Karie > -- > Karie L Reaser A.S. > New Bolton Center > University of Pennsylvania > School of Veterinary Medicine > Comparative Orthopaedic Research Laboratory > 382 W Street Road > Kennett Square, PA. 19348 > Phone:610-925-6278 > Fax:610-925-6820 > Email:kreaser@vet.upenn.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 <@t> earthlink.net Tue May 17 11:43:10 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue May 17 11:43:16 2011 Subject: [Histonet] RELIA Hot Histology Job Alert. Managers, Experienced Techs and New Grads...Check it out!! Message-ID: <0A2C2C4389C24D428AD5C6EDAC678DA9@ownerf1abaad51> Hi Histonetters!! I hope everybody is having a great day. I have a couple of jobs I want to tell you about. These are permanent full time positions and my clients offer great compensation, benefits and relocation assistance. Here are the jobs I want to tell you about: Histology Management: **Asheville, NC **Boston, MA **Baton Rouge, LA Histology Technicians/Technologists: **Long Island, NY - day shift NY license or elig, NEW GRADS WELCOME! **Sarasota, FL - Mohs Tech **Ft. Myers, FL - Dermpath Tech **Portland, OR - Histotech **Portland, ME - Histotech I also need a PA in Charlotte, NC If you or anyone you know is interested in more information about any of these opportunities please contact me at relia1@earthlink.net or toll free at 866-607-3542. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From Pat.Bell <@t> ucdenver.edu Tue May 17 12:14:48 2011 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Tue May 17 12:14:53 2011 Subject: [Histonet] Fixation and Processing of Mouse Lactating Mammary Gland Message-ID: <64DB27005E2FD3439E88502D7A5C9121A4F4206F5E@CORTEZ.ucdenver.pvt> Hello to all, I have some lactating mouse mammary tissue that was fixed in 10% formalin for 24 hours and then stored in 70% alcohol for 1 day before processing. The processing is as follows: 80%-30min, 95%-30min, 95%-40 min, 100% X3-30 min. each, xylene X3-40min. each, and paraffin X3-45min. each. The tissue if very mushy and I am wondering if the tissue was fixed long enough? The tissue is approximately 3mm in thickness but because of the milk in the tissue does it need to fix more or should I process longer? I have embedded the tissue and am wondering how I should go about reprocessing the tissue. Should I go back through xylene to alcohol and then fix for longer and then reprocess or can I melt the blocks and put in fix and then reprocess? I have heard both ways but want to do the method that will not comprise the IHC that needs to be done. Thank you so very much for all of your input and help. Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu From coralmani <@t> yahoo.co.in Tue May 17 12:26:26 2011 From: coralmani <@t> yahoo.co.in (mani kandan) Date: Tue May 17 12:26:32 2011 Subject: [Histonet] ihc on mouse femour Message-ID: <281251.15427.qm@web94715.mail.in2.yahoo.com> hai histonetter, ????? i am doing ihc on mouse femour(bone marrow),i am using nucleostemin antibody to locate cells in proliferation, can any one tried this if u tried please give me the idea about nucleostemin ihc on mouse femour, thank u. M.Manikandan, Researcher, Stemcell unit, King Saud university, Riyadh,KSA +966552012697 From Ronald.Houston <@t> nationwidechildrens.org Tue May 17 12:52:29 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue May 17 12:52:35 2011 Subject: [Histonet] correct CPT code for anal smear Message-ID: What would be the correct CPT code for an anal smear collected in a SurePath vial (thin layer technology) for cytological evaluation and subsequent HPV testing? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From catherinesimonson <@t> gmail.com Tue May 17 13:09:29 2011 From: catherinesimonson <@t> gmail.com (Catherine Simonson) Date: Tue May 17 13:09:33 2011 Subject: [Histonet] Fixation and Processing of Mouse Lactating Mammary Gland In-Reply-To: <64DB27005E2FD3439E88502D7A5C9121A4F4206F5E@CORTEZ.ucdenver.pvt> References: <64DB27005E2FD3439E88502D7A5C9121A4F4206F5E@CORTEZ.ucdenver.pvt> Message-ID: I think longer fixation AND processing. Fix for at least 48 hours in NBF and here is my processing schedule 70% for 45 min 70% for 45 min 95% for 45 min 95% for 45 min 100% for 45 min 100% for 45 min 100% for 45 min xylene for 45 min xylene for 45 min xylene for 45 min paraffin for 45 min paraffin for 45 min paraffin for 45 min paraffin for 45 min Good luck On Tue, May 17, 2011 at 1:14 PM, Bell, Pat wrote: > Hello to all, > > I have some lactating mouse mammary tissue that was fixed in 10% formalin > for 24 hours and then stored in 70% alcohol for 1 day before processing. The > processing is as follows: 80%-30min, 95%-30min, 95%-40 min, 100% X3-30 min. > each, xylene X3-40min. each, and paraffin X3-45min. each. The tissue if very > mushy and I am wondering if the tissue was fixed long enough? The tissue is > approximately 3mm in thickness but because of the milk in the tissue does it > need to fix more or should I process longer? > > I have embedded the tissue and am wondering how I should go about > reprocessing the tissue. Should I go back through xylene to alcohol and then > fix for longer and then reprocess or can I melt the blocks and put in fix > and then reprocess? I have heard both ways but want to do the method that > will not comprise the IHC that needs to be done. > > Thank you so very much for all of your input and help. > Pat > > Pat Bell HT(ASCP) > University of Colorado, Denver > Medical Oncology; MS 8117 > 12801 E 17th Ave. > RC1-S, L18-8402C > Aurora, Co. 80045 > 303-724-6077 > pat.bell@ucdenver.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kstoll <@t> mcw.edu Tue May 17 13:45:41 2011 From: kstoll <@t> mcw.edu (Stoll, Kathryn) Date: Tue May 17 13:44:00 2011 Subject: [Histonet] Paraffin blocks from cell line Message-ID: <110E7925E2B91945A9B79EDFD0DC2B34E29168AE25@MCWMBX2.mcwcorp.net> Hi histonetters, Has anyone made up paraffin blocks from cells from a cell line. This is new to me. I assume they will be in some kind of a media. We will be getting the cells and incubating them to grow. Thanks in advance for the help Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kstoll@mcw.edu From twheelock <@t> mclean.harvard.edu Tue May 17 14:16:12 2011 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Tue May 17 14:16:16 2011 Subject: [Histonet] 100 micron paraffin sections? Message-ID: <4DD2C97C.5090405@mclean.harvard.edu> Hi Everyone: A researcher in our building has done a Golgi-Kopse silver stain on some brain tissue. He says that the tissue has come out brittle with this particular Golgi method. His pieces are 3 mm thick and will easily fit inside of a processing cassette. He would like to embed the tissue in paraffin and cut 100 micron sections. Does anyone have a protocol for processing this kind of tissue into paraffin and then sectioning and mounting such thick 100 micron sections? Thanks, Tim Tim Wheelock Assistant Director, Neuropathology Instructor In Neuroanatomy Harvard Brain Tissue Resource Center 203 Mailman Research Center McLean Hospital Belmont MA 02478 Phone: 617-855-3592 Fax: 617-855-3199 From olstrauss <@t> gmail.com Tue May 17 22:52:54 2011 From: olstrauss <@t> gmail.com (Otto Strauss) Date: Tue May 17 22:52:58 2011 Subject: [Histonet] CD169 for paraffin embedded tissue Message-ID: Hi, I am trying to stain paraffin embedded tissue with CD169, once antigen retrieval has been performed(heat and pressure), but have not have much success. I was wondering if anyone had had any success on paraffin embedded tissue using any specific Ab? Regards, Dr. Otto Strauss University of Auckland From amitapandey <@t> torrentpharma.com Tue May 17 23:29:58 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Tue May 17 23:30:36 2011 Subject: [Histonet] Expiry date of NBF Message-ID: Dear Histonetters, I require one clarification on declaration of expiry date of 4% nutral buffer formalin (NBF) prepared in our lab ( from 40% solution) ? Do you declare the expiry date of this prepared solution ? If so what criteria do you follow for this? Important to mention that we have to archive these tissue in NBF for 5 years as part of GLP toxicology. Your experience or view on these points are welcomed. Amita From amitapandey <@t> torrentpharma.com Tue May 17 23:34:13 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Tue May 17 23:34:44 2011 Subject: [Histonet] Filter Paper for Harris Hematoxylin In-Reply-To: <57297645.8518918.1305643874162.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> References: <57297645.8518918.1305643874162.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> Message-ID: Just continuation of karie query .....Do you suggest different filter paper for Lab prepared hematoxylin? What's your view on lab prepared versus ready made Harris hematoxylin? Dr. Amita Dubey PCSED,TRC From: Karie Reaser To: histonet@lists.utsouthwestern.edu Date: 17/05/11 08:27 PM Subject: [Histonet] Filter Paper for Harris Hematoxylin Sent by: histonet-bounces@lists.utsouthwestern.edu Good morning, What grade filter paper do you use for purchased Harris Hematoxylin filtering? I have used 3 different grades, coarse and still have precipitate on my slides. Any advice is greatly appreciated. Thank you Karie -- Karie L Reaser A.S. New Bolton Center University of Pennsylvania School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 382 W Street Road Kennett Square, PA. 19348 Phone:610-925-6278 Fax:610-925-6820 Email:kreaser@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sabeti_shahram <@t> yahoo.com Wed May 18 00:28:35 2011 From: sabeti_shahram <@t> yahoo.com (Shahram Sabeti) Date: Wed May 18 00:28:41 2011 Subject: [Histonet] congo red staining method for abdominal fat aspirates Message-ID: <76147.20581.qm@web35602.mail.mud.yahoo.com> dear colleagues, ????I have a problem with congo red staining of?abdominal fat aspiration slides.i perform the same way as for slides prepared from?paraffin-embedded blocks, but the results are? poor. ??? thank you in advance for your help. ??? sincerely, ????sabeti? From irena.kirbis <@t> hotmail.com Wed May 18 04:50:31 2011 From: irena.kirbis <@t> hotmail.com (IRENA SREBOTNIK KIRBIS) Date: Wed May 18 04:50:35 2011 Subject: [Histonet] Paraffin blocks from cell line In-Reply-To: <110E7925E2B91945A9B79EDFD0DC2B34E29168AE25@MCWMBX2.mcwcorp.net> References: <110E7925E2B91945A9B79EDFD0DC2B34E29168AE25@MCWMBX2.mcwcorp.net> Message-ID: we made a cell block from cell line by following procedure: - centrifuge cell suspension - fix the sediment overnight in formalin (for tissue fixation) - optional, the button will be anyway fixed by subsequent procedure - centrifuge and decant the formalin - add few drops of liquid agar (not too hot) - as the button solidify you can put it in cassette and process as diagnostic tissue. We got good immunoreactions on sections prepared from melanoma cell line for example. hope this helps Irena Kirbis > From: kstoll@mcw.edu > To: histonet@lists.utsouthwestern.edu > Date: Tue, 17 May 2011 13:45:41 -0500 > Subject: [Histonet] Paraffin blocks from cell line > > Hi histonetters, > > Has anyone made up paraffin blocks from cells from a cell line. This is new to me. I assume they will be in some kind of a media. We will be getting the cells and incubating them to grow. > Thanks in advance for the help > > Kathryn Stoll, HT(ASCP) > Depatment of Pathology > Medical College of Wisconsin > 9200 W Wisconsin Ave > Milwaukee WI 53226 > 414.805.1525 > kstoll@mcw.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Wed May 18 07:45:32 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed May 18 07:45:36 2011 Subject: [Histonet] Paraffin blocks from cell line In-Reply-To: References: <110E7925E2B91945A9B79EDFD0DC2B34E29168AE25@MCWMBX2.mcwcorp.net> Message-ID: <002201cc1559$799ebaa0$6cdc2fe0$@northwestern.edu> You can use Histogel as well from Richard Allan (Fisher or VWR) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of IRENA SREBOTNIK KIRBIS Sent: Wednesday, May 18, 2011 4:51 AM To: kstoll@mcw.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin blocks from cell line we made a cell block from cell line by following procedure: - centrifuge cell suspension - fix the sediment overnight in formalin (for tissue fixation) - optional, the button will be anyway fixed by subsequent procedure - centrifuge and decant the formalin - add few drops of liquid agar (not too hot) - as the button solidify you can put it in cassette and process as diagnostic tissue. We got good immunoreactions on sections prepared from melanoma cell line for example. hope this helps Irena Kirbis > From: kstoll@mcw.edu > To: histonet@lists.utsouthwestern.edu > Date: Tue, 17 May 2011 13:45:41 -0500 > Subject: [Histonet] Paraffin blocks from cell line > > Hi histonetters, > > Has anyone made up paraffin blocks from cells from a cell line. This is new to me. I assume they will be in some kind of a media. We will be getting the cells and incubating them to grow. > Thanks in advance for the help > > Kathryn Stoll, HT(ASCP) > Depatment of Pathology > Medical College of Wisconsin > 9200 W Wisconsin Ave > Milwaukee WI 53226 > 414.805.1525 > kstoll@mcw.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aj.taylor <@t> blueyonder.co.uk Wed May 18 08:04:40 2011 From: aj.taylor <@t> blueyonder.co.uk (taylor alan) Date: Wed May 18 08:04:45 2011 Subject: [Histonet] Filter Paper for Harris Hematoxylin In-Reply-To: References: <57297645.8518918.1305643874162.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> Message-ID: Hi Dr Dubey Thanks for your message regarding choice of filter papers. Here in the U.K. Whatman No 1 is almost universally used for filtration of all reagents and solutions used in microscopy or general chemistry preparation. Other grades of filter paper are available for other applications, but No 1 is the most popular and enduring simple filtering medium for our purposes. I do not have any particular views on the differences between purchased or 'in-house' prepared Harris Haematoxylin solutions. We use both. We use Sodium Iodate as the ripening agent, this does not appear to change the qualities of the finished solution over the previous use of mercuric oxide. We change our haematoxylin once a week on the stainer, usually it is one of the last jobs at the end of the day to set up the filtering of used haematoxylin in the trough, (usually approx 400ml) so that in the morning there is a flask of crystal free stain to re-use , this does not apply to weekends, as the haematoxylin is discarded early monday morning, the trough washed and dried and fresh haematoxylin added, we run several test slides for evaluation. In addition we always routinely change all the other solutions on the stainer at the start of the week and as required subsequently. Hope this is helpful to you. Alan Taylor BSc(Hons). FRMS. Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter. EX1 3AJ On 18 May 2011 05:34, wrote: > Just continuation of karie query .....Do you suggest different filter > paper for Lab prepared hematoxylin? What's your view on lab prepared > versus ready made Harris hematoxylin? > > Dr. Amita Dubey > PCSED,TRC > > > > From: Karie Reaser > To: histonet@lists.utsouthwestern.edu > Date: 17/05/11 08:27 PM > Subject: [Histonet] Filter Paper for Harris Hematoxylin > Sent by: histonet-bounces@lists.utsouthwestern.edu > > > > > Good morning, > What grade filter paper do you use for purchased Harris Hematoxylin > filtering? I have used 3 different grades, coarse and still have > precipitate on my slides. Any advice is greatly appreciated. Thank you > Karie > -- > Karie L Reaser A.S. > New Bolton Center > University of Pennsylvania > School of Veterinary Medicine > Comparative Orthopaedic Research Laboratory > 382 W Street Road > Kennett Square, PA. 19348 > Phone:610-925-6278 > Fax:610-925-6820 > Email:kreaser@vet.upenn.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mjdessoye <@t> wvhcs.org Wed May 18 09:00:11 2011 From: mjdessoye <@t> wvhcs.org (Dessoye, Michael J) Date: Wed May 18 09:00:18 2011 Subject: [Histonet] Ventana E-cadherin problem Message-ID: Hello Histonet, We've been having a recurring problem with E-cadherin from Ventana on Benchmark Ultra. It's spanned two different lots of antibody. Our tissues are suddenly falling off the slides. Negative controls are ok, just seems to be after antibody treatment. The protocol hasn't changed, it's the manufacturer recommended protocol. It also seems to happen to various tissue types and fixation times. We've tried drying the slides longer and it doesn't seem to help. Anyone else having problems with this antibody? Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From shive003 <@t> umn.edu Wed May 18 09:08:10 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed May 18 09:08:16 2011 Subject: [Histonet] esterase stain Message-ID: <30BB21533B364FE699C7B0110F60E07E@auxs.umn.edu> Good Morning, I'm posting this for a non-member. Does anyone know of a lab that does nonspecific esterase staining? A client needs it done on mouse tissue, and we don't perform that test here in our histo lab. Jan Shivers University of Minnesota Veterinary Diagnostic Laboratory shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) From wbenton <@t> cua.md Wed May 18 09:05:03 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Wed May 18 09:08:26 2011 Subject: [Histonet] RE: Ventana E-cadherin problem In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD91D6EC7CE5F@CUAEXH1.GCU-MD.local> Sounds to me like you may want to check your slide lots to make sure the charge is consistent. I have purchased slides were there was variability within the back from slide to slide. Also, if you use CC1 and CC2 I would check to make sure that the solutions were not inadvertently switched, since harsher solutions tend to wreak havoc on samples. You may want to pH them as well to make sure they have broken down. Tech support will give you the pH range for each solution if you don't already know them. I hope one of these will resolve your issue. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdessoye@wvhcs.org] Sent: Wednesday, May 18, 2011 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana E-cadherin problem Hello Histonet, We've been having a recurring problem with E-cadherin from Ventana on Benchmark Ultra. It's spanned two different lots of antibody. Our tissues are suddenly falling off the slides. Negative controls are ok, just seems to be after antibody treatment. The protocol hasn't changed, it's the manufacturer recommended protocol. It also seems to happen to various tissue types and fixation times. We've tried drying the slides longer and it doesn't seem to help. Anyone else having problems with this antibody? Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From Melissa.Kuhnla <@t> chsli.org Wed May 18 09:23:43 2011 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Wed May 18 09:23:48 2011 Subject: [Histonet] RE: Ventana E-cadherin problem In-Reply-To: <0B8979A204680A42B93A52B486088CD91D6EC7CE5F@CUAEXH1.GCU-MD.local> References: <0B8979A204680A42B93A52B486088CD91D6EC7CE5F@CUAEXH1.GCU-MD.local> Message-ID: We also experienced tissue adhesion problems. We have done several things to address the issue: We wear gloves when cutting controls to decrease any grease from our fingers, we also dip the slide into the water bath upside down as to leave the portion of the slide for the patient section untouched or un-dipped. Whenever repeating a slide because the first attempt fell off...we always mount the section on a slide all by itself (no control). We have switched to superfrost excel adhesion slides from fisher. (we have also experienced a bad lot of slides):( -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Wednesday, May 18, 2011 10:05 AM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ventana E-cadherin problem Sounds to me like you may want to check your slide lots to make sure the charge is consistent. I have purchased slides were there was variability within the back from slide to slide. Also, if you use CC1 and CC2 I would check to make sure that the solutions were not inadvertently switched, since harsher solutions tend to wreak havoc on samples. You may want to pH them as well to make sure they have broken down. Tech support will give you the pH range for each solution if you don't already know them. I hope one of these will resolve your issue. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdessoye@wvhcs.org] Sent: Wednesday, May 18, 2011 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana E-cadherin problem Hello Histonet, We've been having a recurring problem with E-cadherin from Ventana on Benchmark Ultra. It's spanned two different lots of antibody. Our tissues are suddenly falling off the slides. Negative controls are ok, just seems to be after antibody treatment. The protocol hasn't changed, it's the manufacturer recommended protocol. It also seems to happen to various tissue types and fixation times. We've tried drying the slides longer and it doesn't seem to help. Anyone else having problems with this antibody? Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From laurie.colbert <@t> huntingtonhospital.com Wed May 18 10:26:37 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed May 18 10:26:41 2011 Subject: [Histonet] RE: Ventana E-cadherin problem In-Reply-To: References: <0B8979A204680A42B93A52B486088CD91D6EC7CE5F@CUAEXH1.GCU-MD.local> Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AC7C@EXCHANGE3.huntingtonhospital.com> We've always had problems with the tissue falling off on the E-Cad (Ventana). Normally, we put the patient tissue on the same slide as the control tissue - but for the E-Cad, we always put the patient tissue on a slide all by itself. This doesn't completely solve the problem, but it's better. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, May 18, 2011 7:24 AM To: Walter Benton; Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Ventana E-cadherin problem We also experienced tissue adhesion problems. We have done several things to address the issue: We wear gloves when cutting controls to decrease any grease from our fingers, we also dip the slide into the water bath upside down as to leave the portion of the slide for the patient section untouched or un-dipped. Whenever repeating a slide because the first attempt fell off...we always mount the section on a slide all by itself (no control). We have switched to superfrost excel adhesion slides from fisher. (we have also experienced a bad lot of slides):( -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Wednesday, May 18, 2011 10:05 AM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ventana E-cadherin problem Sounds to me like you may want to check your slide lots to make sure the charge is consistent. I have purchased slides were there was variability within the back from slide to slide. Also, if you use CC1 and CC2 I would check to make sure that the solutions were not inadvertently switched, since harsher solutions tend to wreak havoc on samples. You may want to pH them as well to make sure they have broken down. Tech support will give you the pH range for each solution if you don't already know them. I hope one of these will resolve your issue. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdessoye@wvhcs.org] Sent: Wednesday, May 18, 2011 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana E-cadherin problem Hello Histonet, We've been having a recurring problem with E-cadherin from Ventana on Benchmark Ultra. It's spanned two different lots of antibody. Our tissues are suddenly falling off the slides. Negative controls are ok, just seems to be after antibody treatment. The protocol hasn't changed, it's the manufacturer recommended protocol. It also seems to happen to various tissue types and fixation times. We've tried drying the slides longer and it doesn't seem to help. Anyone else having problems with this antibody? Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Wed May 18 10:35:09 2011 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Wed May 18 10:35:16 2011 Subject: [Histonet] RE: Ventana E-cadherin problem In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2AC7C@EXCHANGE3.huntingtonhospital.com> References: <0B8979A204680A42B93A52B486088CD91D6EC7CE5F@CUAEXH1.GCU-MD.local> <57BE698966D5C54EAE8612E8941D76830AD2AC7C@EXCHANGE3.huntingtonhospital.com> Message-ID: We treat certain antibodies (including E-Cad, and many other breast markers) by putting the slides into formalin for 15 minutes after baking. Then, rinse in tap and put on the machine. It helps keep tissue on the slides. Also, as an aside, I heard directly from someone who works for Erie (who makes superfrost plus slides)that you cannot remove the positive charge from the slide, no matter how many times you dip it in water - as long as it's plain distilled water, and doesn't have any additives in it (such as Sta-on). That being said, we had many issues with bad lots of slides where the positive charge was uneven, resulting in poor staining more often than with tissue adhesion. Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, May 18, 2011 11:27 AM To: Kuhnla, Melissa; Walter Benton; Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Ventana E-cadherin problem We've always had problems with the tissue falling off on the E-Cad (Ventana). Normally, we put the patient tissue on the same slide as the control tissue - but for the E-Cad, we always put the patient tissue on a slide all by itself. This doesn't completely solve the problem, but it's better. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, May 18, 2011 7:24 AM To: Walter Benton; Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Ventana E-cadherin problem We also experienced tissue adhesion problems. We have done several things to address the issue: We wear gloves when cutting controls to decrease any grease from our fingers, we also dip the slide into the water bath upside down as to leave the portion of the slide for the patient section untouched or un-dipped. Whenever repeating a slide because the first attempt fell off...we always mount the section on a slide all by itself (no control). We have switched to superfrost excel adhesion slides from fisher. (we have also experienced a bad lot of slides):( -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Wednesday, May 18, 2011 10:05 AM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ventana E-cadherin problem Sounds to me like you may want to check your slide lots to make sure the charge is consistent. I have purchased slides were there was variability within the back from slide to slide. Also, if you use CC1 and CC2 I would check to make sure that the solutions were not inadvertently switched, since harsher solutions tend to wreak havoc on samples. You may want to pH them as well to make sure they have broken down. Tech support will give you the pH range for each solution if you don't already know them. I hope one of these will resolve your issue. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdessoye@wvhcs.org] Sent: Wednesday, May 18, 2011 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana E-cadherin problem Hello Histonet, We've been having a recurring problem with E-cadherin from Ventana on Benchmark Ultra. It's spanned two different lots of antibody. Our tissues are suddenly falling off the slides. Negative controls are ok, just seems to be after antibody treatment. The protocol hasn't changed, it's the manufacturer recommended protocol. It also seems to happen to various tissue types and fixation times. We've tried drying the slides longer and it doesn't seem to help. Anyone else having problems with this antibody? Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Wed May 18 11:46:37 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed May 18 11:46:55 2011 Subject: [Histonet] Leica Bond III Message-ID: <006301cc157b$2827cf00$78776d00$@com> Hi Histonetters I am testing the Leica Bond III immunostainer. How is everyone handling processing the Hercept test from Dako on the Bond III? I cannot use either the approved pretreatment or visualization system, not to mention the approved buffer. I currently run my immunos on the Dako so it has not been a problem. I would appreciate any input anyone has. Thanks in advance. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 Ext. 232 e-mail cpyse@x-celllab.com From DKBoyd <@t> chs.net Wed May 18 12:02:02 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed May 18 12:04:10 2011 Subject: [Histonet] Stomach Sleeve Reduction CPT Message-ID: What CPT code is everyone using for stomach sleeve reduction surgery for morbid obesity? Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From algranth <@t> email.arizona.edu Wed May 18 12:11:34 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed May 18 12:11:49 2011 Subject: [Histonet] mission project Message-ID: <252BC967-A161-43A2-A1D8-C85A7005C64D@email.arizona.edu> Last year I was lucky to be a part of a medical mission in Belize. While there I assisted a dermatologist. This year we are looking for an additional derm dr. (maybe one who feels comfortable reading out slides) and a plastic surgeon to expand on the medical part. There are family practice and ER docs who also are part of the team. On the dental side - there are already numerous dentists and dental techs and hygienists who participate. We also have audiologists and occupational therapists. Dates for the trips are in the fall near the end of October and beginning of November. If anybody knows of a dermatologist or plastic surgeon please ask them to contact me. Belize is a beautiful country and the people are lovely and very friendly. This project is well organized and is well received by Belizeans. Web site is http://www.belizemissionproject.com/main.html Thanks! Andi Grantham U of A, Dept of Cellular and Molecular Medicine 520-626-4415 algranth@email.arizona.edu From Candy.A.Bales <@t> uth.tmc.edu Wed May 18 12:16:42 2011 From: Candy.A.Bales <@t> uth.tmc.edu (Bales, Candy A) Date: Wed May 18 12:16:47 2011 Subject: [Histonet] charge question Message-ID: <62C915811DD5A142851D95CA6BC5D1E41B39A48202@UTHCMS1.uthouston.edu> For those who stain smears, if you stain with PAS/PAP, would/do you charge for one stain or two. And if you have more that one smear, do you charge for each smear? Thank you Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax From Kathy.Bonness <@t> UTSouthwestern.edu Wed May 18 13:23:27 2011 From: Kathy.Bonness <@t> UTSouthwestern.edu (Kathy Bonness) Date: Wed May 18 13:23:30 2011 Subject: [Histonet] Looking for Aperio or similar Slide Scanner Message-ID: <2E046FFADED6054CB6DEB42B1B3E09642096FE@swmsmail6.swmed.org> Hello, I am looking for a slide scanner like Aperio to scan my lung tissue slides at high quality and inclusive of increasingly higher magnification objectives. I have the lite version of Aperio to view slides I scanned in when training at MD Anderson in Houston using their Aperio and would like to find something similar in the Dallas area if not at UTSW itself. If you have any suggestions of who has this system or one like it that also allows others to use and/or pay to do so, please let me know. Also, if you have suggestions on purchasing one instead or any other commentaries, I would love to get some feedback. Thank you for your time and assistance, Kathy Kathy Bonness, PhD. kathy.bonness@utsouthwestern.edu UTSW Dallas, TX Green Center for Systems Biology Department of Pharmacology Altschuler/Wu Lab ND9.214 ________________________________ UT Southwestern Medical Center The future of medicine, today. From liz <@t> premierlab.com Wed May 18 13:29:26 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed May 18 13:29:30 2011 Subject: [Histonet] Looking for Aperio or similar Slide Scanner In-Reply-To: <2E046FFADED6054CB6DEB42B1B3E09642096FE@swmsmail6.swmed.org> Message-ID: Kathy We have an Aperio Scanscope XT and scan for a fee Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy Bonness Sent: Wednesday, May 18, 2011 12:23 PM To: histonet@lists.utsouthwestern.edu Cc: Kathy Bonness Subject: [Histonet] Looking for Aperio or similar Slide Scanner Hello, I am looking for a slide scanner like Aperio to scan my lung tissue slides at high quality and inclusive of increasingly higher magnification objectives. I have the lite version of Aperio to view slides I scanned in when training at MD Anderson in Houston using their Aperio and would like to find something similar in the Dallas area if not at UTSW itself. If you have any suggestions of who has this system or one like it that also allows others to use and/or pay to do so, please let me know. Also, if you have suggestions on purchasing one instead or any other commentaries, I would love to get some feedback. Thank you for your time and assistance, Kathy Kathy Bonness, PhD. kathy.bonness@utsouthwestern.edu UTSW Dallas, TX Green Center for Systems Biology Department of Pharmacology Altschuler/Wu Lab ND9.214 ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Wed May 18 13:49:49 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Wed May 18 13:49:55 2011 Subject: [Histonet] Re: 100 micron paraffin sections? Message-ID: Tim, The thickest paraffin sections I have cut are 50 microns and they curled so tightly right off the blade I can't imagine what you might get with a 100 micron section. Even if you do get this slab intact, I think the tissue would show cracking artifact, not to mention the big chunks taken out at the block face like one would get from too aggressive rough trimming (facing into the block). Sections of this thickness are almost always better if done with a vibratome and mounted out of buffer onto a glass slide. Good luck! Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO From amber.mckenzie <@t> gastrodocs.net Wed May 18 14:36:11 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed May 18 14:35:53 2011 Subject: [Histonet] QC Manual Message-ID: <03C921A1EAF7F541B16543F6EC6A4B370421C425@giamail2.Gia.com> I am updating my QC Manual and wanted to know if you QC your Processors and Microtomes? These are the only 2 pieces of equipment that I haven't been QC'ing. Here's what I QC each month, am I covering everything? 1. Eye wash station 2. Fridge temp 3. 10%formalin 4. Microscope 5. Processor alarm report 6. Embedder 7. H&E stainer 8. Coverslipper 9. Special Stainers 10. Immuno stainers 11. Buffer's 12. Control slides 13. Water baths From MSHERWOOD <@t> PARTNERS.ORG Wed May 18 14:47:16 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed May 18 14:47:20 2011 Subject: [Histonet] Formalin fixed frozen sections Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5752@PHSXMB30.partners.org> Does anyone fix their frozen tissue first in formalin before embedding and sectioning? I know one investigator in our lab did that previously, but was not sure of the strength of formalin (I don't believe it was 10%). Another investigator inquired about doing that. I would appreciate hearing from fellow histonetters. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rjbuesa <@t> yahoo.com Wed May 18 15:03:14 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 18 15:03:17 2011 Subject: [Histonet] QC Manual In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B370421C425@giamail2.Gia.com> References: <03C921A1EAF7F541B16543F6EC6A4B370421C425@giamail2.Gia.com> Message-ID: <286356.4217.qm@web65704.mail.ac4.yahoo.com> If you have a fumes hood, and a body emergency shower you should QC them along with your drying oven microtomes and tissue processor. Any balance you may have also should be QC'd. Ren? J. From: Amber McKenzie To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 18, 2011 3:36 PM Subject: [Histonet] QC Manual I am updating my QC Manual and wanted to know if you QC your? Processors and? Microtomes?? These are the only 2 pieces of equipment that I haven't been QC'ing. Here's what I QC each month, am I covering everything? 1.? ? ? Eye wash station 2.? ? ? Fridge temp 3.? ? ? 10%formalin 4.? ? ? Microscope 5.? ? ? Processor alarm report 6.? ? ? Embedder 7.? ? ? H&E stainer 8.? ? ? Coverslipper 9.? ? ? Special Stainers 10.? Immuno stainers 11.? Buffer's 12.? Control slides 13.? Water baths _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 18 15:06:56 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 18 15:07:01 2011 Subject: [Histonet] Formalin fixed frozen sections In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5752@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5752@PHSXMB30.partners.org> Message-ID: <849668.97214.qm@web65707.mail.ac4.yahoo.com> Formalin fixed FS before embedding and sectioning? That is quite confuse. Your FS should be fixed before H&E staining (acetone or even 10% NBF will do). If you refer to the specimen that was frozen to do the FS, before being embedded and cut, has to be fixed to become a FFPE specimen. Ren? J. From: "Sherwood, Margaret " To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 18, 2011 3:47 PM Subject: Re: [Histonet] Formalin fixed frozen sections Does anyone fix their frozen tissue first in formalin before embedding and sectioning?? I know one investigator in our lab did that previously, but was not sure of the strength of formalin (I don't believe it was 10%).? Another investigator inquired about doing that. I would appreciate hearing from fellow histonetters. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed May 18 14:51:11 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed May 18 15:08:06 2011 Subject: [Histonet] Formalin fixed frozen sections In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5752@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5752@PHSXMB30.partners.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707011087@SMCMAIL01.somerset-healthcare.com> Not before embedding in OCT, but we have 10%nbf as our first station in the staining process. The slides stay in there for 1 minute, water rinse, then proceed with your stain as usual. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, May 18, 2011 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin fixed frozen sections Does anyone fix their frozen tissue first in formalin before embedding and sectioning? I know one investigator in our lab did that previously, but was not sure of the strength of formalin (I don't believe it was 10%). Another investigator inquired about doing that. I would appreciate hearing from fellow histonetters. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From TJJ <@t> stowers.org Wed May 18 15:51:53 2011 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Wed May 18 15:51:58 2011 Subject: [Histonet] Re: Formalin fixed frozen sections Message-ID: Peggy, We fix ours prior to freezing and cryosectioning routinely. You can use 10% NBF (commercial prep) or you can make your own using paraformaldehyde, 4% in PBS buffer. After fixation, you will need to cryoprotect in sucrose/PBS. We fix, then rinse, and then place in 15% sucrose/PBS until the sample sinks. We then place it in 30% sucrose/PBS until the sample sinks. We will put it into a cryomold with OCT and let sit for some time, then a fresh change and snap freeze. The tissues section very well. Good luck! Teri From saby_joseph_a <@t> yahoo.com Wed May 18 16:46:59 2011 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Wed May 18 16:47:02 2011 Subject: [Histonet] Expiry date of NBF In-Reply-To: References: Message-ID: <184595.90354.qm@web114411.mail.gq1.yahoo.com> We use the expiration date on the formalin as the use by date.? By the time the solution would be out of date, the tissues should be very well fixed, so the formalin becomes just a holding solution. Joe Saby NAMSA ________________________________ From: "amitapandey@torrentpharma.com" To: histonet@lists.utsouthwestern.edu Sent: Wed, May 18, 2011 12:29:58 AM Subject: [Histonet] Expiry date of NBF Dear Histonetters, I require one clarification on declaration of? expiry date of 4% nutral buffer formalin (NBF) prepared in our lab ( from 40% solution) ? Do you declare the expiry date of this prepared solution ? If so what criteria do you follow for this? Important to mention that? we have to archive these tissue in NBF for 5 years as part of GLP toxicology. Your experience or view on these points are welcomed. Amita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adesupo2002 <@t> hotmail.com Wed May 18 18:31:37 2011 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Wed May 18 18:31:40 2011 Subject: [Histonet] Histotech opening in Norman, OK In-Reply-To: <3396079.1305760287531.JavaMail.nobody@nosuchhost.nosuchdomain.com> References: <3396079.1305760287531.JavaMail.nobody@nosuchhost.nosuchdomain.com> Message-ID: Job Title: Histology Technician fulltime Porter campus - $3000 Sign On Bonus Department: 40100 -- Laboratory Job Type: Full Time Shift Info: Mon-Fri/ 2 half day Saturday shifts/mon Expertise: Allied Health Job Description: Performs histology procedures including embedding and cutting tissue specimens and preparing and staining microscopic slides. Works with supervisor to assure section is maintained according to regulatory standards. Job Requirements: **Completion of a CAHEA accredited Histotechnology program; or **Associate degree of at least 60 semester hours of academic credit from an accredited college / university including 6 semester hours in chemistry and 6 semester hours in biology; and 1 year full time experience in histopathology under the supervision of a certified pathologist; or **High school graduation or equivalent and two years full time experience in histopathology under the supervision of a certified pathologist. One year of hospital experience as a histologic technician in all phases of histology, or equivalent. Registration as a Histologic Technician (HT) or Histologic Technologist (HTL) by the American society of Clinical Pathologists, or eligibility for registration. Registration is preferred. If interesting, apply on line at www.normanregional.com Adesupo Adesuyi. BS, HT (ASCP) HTL, QIHC (ASCP) Histology Supervisor Norman Regional Health System 405 - 307 -1145 From sherrineely <@t> hotmail.com Wed May 18 18:56:36 2011 From: sherrineely <@t> hotmail.com (sherri neely) Date: Wed May 18 18:56:39 2011 Subject: [Histonet] haz waste Message-ID: Does anyone have a letter from there waste water municipality accepting any of the chemicals used in the histo lab (alcohol or neutralized 10%NBF)? Does any one treat there 10%NBF and dispose of it down the sink? Have A Great Day! Sherri Neely From amitapandey <@t> torrentpharma.com Wed May 18 23:02:23 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Wed May 18 23:02:58 2011 Subject: [Histonet] Formalin fixed frozen sections In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5752@PHSXMB30.partners.org> References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5752@PHSXMB30.partners.org> Message-ID: We take frozen section from formalin fixed tissue and placed them in 30% sucrose overnight before mounting on chucks. But never tried the other way round frozen tissue then fixed formalin, i heard that it helps in falling of the tissue. But no experience. Amita From: "Sherwood, Margaret " To: Date: 19/05/11 01:21 AM Subject: Re: [Histonet] Formalin fixed frozen sections Sent by: histonet-bounces@lists.utsouthwestern.edu Does anyone fix their frozen tissue first in formalin before embedding and sectioning? I know one investigator in our lab did that previously, but was not sure of the strength of formalin (I don't believe it was 10%). Another investigator inquired about doing that. I would appreciate hearing from fellow histonetters. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sara.Lees <@t> covance.com Thu May 19 03:27:34 2011 From: Sara.Lees <@t> covance.com (Lees, Sara) Date: Thu May 19 03:27:52 2011 Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Message-ID: <248907F494F5B740A86B9C6FF4C3D59F5F12D2@crwxch04.ent.covance.com> Hi I have just diluted my DPX with xylene and my god its working a treat, thanks to all who helped me your very kind. fingers crossed it keeps this up!!!!!! Thanks Sara _____ From: Paula Pierce [mailto:contact@excaliburpathology.com] Sent: Tuesday, May 17, 2011 3:37 PM To: Lees, Sara; Histonet Subject: Re: [Histonet] Sakura Tissue-tek Glas Air bubble problems Thin the mounting media with xylene or solvent you use. I use Permount thinned 60/40 with xylene. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com _____ From: "Lees, Sara" To: "histonet@lists.utsouthwestern.edu" Sent: Tue, May 17, 2011 9:17:37 AM Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems Hi All, I am having horrible problems with air bubbles , can anyone help I have changed the speed increased the mountant volume, clean it, kissed it, u name it and nothing has worked...Can anyone help Thanks Sara ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From Amanda.Phipps <@t> nationwidechildrens.org Thu May 19 08:30:27 2011 From: Amanda.Phipps <@t> nationwidechildrens.org (Phipps, Amanda) Date: Thu May 19 08:30:35 2011 Subject: [Histonet] Alizarin Red Troubleshooting Message-ID: <20CE7246011C12489BA04BEB85CCDA1F0351BF252B@res2k7ms01> Hi All We are currently trying to stain for calcium deposits using Alizarin Red. A few months ago our protocol worked fine, but this time we are getting zero staining. Reagent has been made fresh, and pH is between 4.1 and 4.2. Here is our protocol. Deparaffinize to distilled H20 Stain slides with Alizarin Red S for 30 seconds to 5 minutes and observe reaction microscopically Shake off excess dye and blot sections Dehydrate in acetone 10 seconds to 20 seconds, then acetone-xylene (1:1) solution 10-20 seconds Clear in xylene and mount in synthetic mounting medium. Any help is greatly appreciated!! Amanda Phipps, HTL (ASCP) Histotechnologist Morphology Core Lab The Research Institute at Nationwide Children's Hospital 700 Children's Drive Columbus, Ohio 43205 (614) 355-3474 ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Robin.Taylor <@t> prexushealth.com Thu May 19 08:31:21 2011 From: Robin.Taylor <@t> prexushealth.com (Taylor, Robin) Date: Thu May 19 08:31:26 2011 Subject: [Histonet] (no subject) Message-ID: <884A8E132D88314EA5E1FC3BBCA3DA2E9EA53A9288@PHPEXMBCLUS.docsgroup.com> I have a new carbon filter for a Leica Coverslipper Model CV5000 that I ordered by mistake. It is from Mercedes Medical, part #MER440122. It is free to whoever will pay the shipping to your facility. Thanks, Robin Taylor HT Butler Cty Medical Center 3145 Hamilton ason Rd. Hamilton, OH 45011 513-454-1417 ________________________________ This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From JThawley <@t> ShoreMemorial.org Thu May 19 08:45:44 2011 From: JThawley <@t> ShoreMemorial.org (JThawley@ShoreMemorial.org) Date: Thu May 19 08:46:37 2011 Subject: [Histonet] Question Message-ID: We currently have a Ventana Benchmark XT and are looking at the Leica Bond III. Has anyone made the transition from a Ventana to a Leica recently and how difficult was it? Jennifer Thawley HT, ASCP Histology Supervisor Shore Memorial Hospital (609) 653-3577 ext. 2084 This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. From gonavy2003 <@t> gmail.com Thu May 19 09:51:10 2011 From: gonavy2003 <@t> gmail.com (Rick T.) Date: Thu May 19 09:51:35 2011 Subject: [Histonet] Re: Filter Paper for Harris Hematoxylin Message-ID: <001801cc1634$3a81fe50$af85faf0$@gmail.com> Karie, Just a little input from a newbie. I am researching stains and fixatives for causes of error in one of my courses. For the hematoxylin precipitate attributed to inadequate filtration, I found a site that discussed this, along with formalin pigment, mainly that sometimes precipitate that is believed to come from the hematoxylin, actually can be caused by other things.here is the site to check it out: http://www.scientistsolutions.com/t9487-persistent+problem+of+formalin+cryst als+in+the+h_e+stained+tissue+sections.html It may not be your filters. Just a thought. Rick T. From gonavy2003 <@t> gmail.com Thu May 19 10:21:37 2011 From: gonavy2003 <@t> gmail.com (Rick T.) Date: Thu May 19 10:21:50 2011 Subject: [Histonet] RE: Histonet Digest, Vol 90, Issue 20 In-Reply-To: <4dd3fb3b.41b4ec0a.0260.0a45SMTPIN_ADDED@mx.google.com> References: <4dd3fb3b.41b4ec0a.0260.0a45SMTPIN_ADDED@mx.google.com> Message-ID: <003701cc1638$74d29840$5e77c8c0$@gmail.com> For Karie's query; I have just a little input from a newbie. I am researching stains and fixatives for causes of error in one of my courses. For the hematoxylin precipitate attributed to inadequate filtration, I found a site that discussed this, along with formalin pigment, mainly that sometimes precipitate that is believed to come from the hematoxylin, actually can be caused by other things.here is the site to check it out: http://www.scientistsolutions.com/t9487-persistent+problem+of+formalin+cryst als+in+the+h_e+stained+tissue+sections.html It may not be your filters. Just a thought. Rick T. From Bauer.Karen <@t> mayo.edu Thu May 19 10:28:35 2011 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Thu May 19 10:28:37 2011 Subject: [Histonet] Continuous processing Message-ID: <53FC421CC200C5429929EDE6C3676F30015DAE19@msgebe34> Hi all... I'd like to get a quick reply on what everyone considers is the best continuous processor out on the market. I'd like to start processing the small biopsies continually throughout the day and I've been thinking about the Tissue-Tek Xpress or Xpress 50. Comments and suggestions are greatly appreciated. Thank you, Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System bauer.karen@mayo.edu From c.tague <@t> pathologyarts.com Thu May 19 11:04:24 2011 From: c.tague <@t> pathologyarts.com (Curt Tague) Date: Thu May 19 11:04:58 2011 Subject: [Histonet] IHC pos. & neg. control question Message-ID: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt From LSebree <@t> uwhealth.org Thu May 19 11:28:20 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu May 19 11:29:02 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> References: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> Message-ID: That's a new one on me! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Thu May 19 11:45:45 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu May 19 11:45:49 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: <-731888389188763398@unknownmsgid> References: <-731888389188763398@unknownmsgid> Message-ID: Curt, Nonsense. The negative control is used to evaluate endogenous staining in the *patient* tissue. Your Pathologist needs to do another residency at clown college. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) From NMargaryan <@t> childrensmemorial.org Thu May 19 11:55:14 2011 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu May 19 11:58:27 2011 Subject: [Histonet] FW: How to remove DAB to restain with DAB Message-ID: Hello, I would like to repeat my DAB staining on the same slide with DAB I run before. I know that "acid alcohol" will remove hematoxylin but how to remove DAB? I appreciate to any suggestion. Thanks in advance, Naira From silvinamolinuevo <@t> yahoo.com.ar Thu May 19 12:02:52 2011 From: silvinamolinuevo <@t> yahoo.com.ar (Silvina Molinuevo) Date: Thu May 19 12:02:57 2011 Subject: [Histonet] Alizarin Red Troubleshooting Message-ID: <357251.49169.qm@web113601.mail.gq1.yahoo.com> Dear Amanda, if you have the staining solution well prepared (the colour must be like dark iodide solutions, I recomend you to check ammonia solutions) the problem should be that you are loosening your calcium deposits. May be you have sth wrong or may be some EDTA contamination in the fixative or hydrating and dehydrating solutions. Best regards, Dr Mar?a Silvina Molinuevo Grupo de Investigacion en Osteopatias y Metabolismo Mineral Departamento de Ciencias Biologicas Facultad de Ciencias Exactas Universidad Nacional de La Plata 47 y 115 (1900)La Plata Argentina www.biol.unlp.edu.ar/giomm e-mail: silvina.molinuevo@bigfoot.com From mwich <@t> 7thwavelabs.com Thu May 19 12:08:38 2011 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu May 19 12:08:45 2011 Subject: [Histonet] BrdU on rat tissue Message-ID: <62A8156F8071C8439080D626DF8C33A6017A9D04@wave-mail.7thwave.local> Can anyone recommend a good commercially available kit for BrdU on rat tissue? Thanks in advance for any suggestions. From sbaldwin <@t> mhhcc.org Thu May 19 12:11:08 2011 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Thu May 19 12:11:15 2011 Subject: [Histonet] haz mat Message-ID: Our municipality actually came here and told us not to put anything down the drains. We now have it all hauled off they told us we can't even put the 10% when it has been treated down the drain. Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. From c.tague <@t> pathologyarts.com Thu May 19 12:23:31 2011 From: c.tague <@t> pathologyarts.com (Curt Tague) Date: Thu May 19 12:23:35 2011 Subject: [Histonet] haz mat In-Reply-To: References: Message-ID: <01a601cc1649$79ad83a0$6d088ae0$@tague@pathologyarts.com> I think that depends on the state you're in and what the regs are. I'll forward this to my vendor who makes our formalin neutralizer, He may be able to help out. There are several different products I think but not all are state approved. I had to find the one that is approved here, in cali. Curt -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Thursday, May 19, 2011 10:11 AM To: histo net Subject: [Histonet] haz mat Our municipality actually came here and told us not to put anything down the drains. We now have it all hauled off they told us we can't even put the 10% when it has been treated down the drain. Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jm.lapointe <@t> accellab.com Thu May 19 12:25:49 2011 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Thu May 19 12:25:53 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: <201105191632.p4JGWS8w032657@gateway7.lastspam.com> References: <201105191632.p4JGWS8w032657@gateway7.lastspam.com> Message-ID: Hi Curt, I agree with your pathologist. The section that you use as a negative control (without primary) in an IHC run should ideally be tissue that is a known positive, and of the same nature as the test sample. Therefore the best sample is often a section of your positive control tissue. The purpose of this negative control is to make sure that any positive staining observed in the test sample is not due to a spurious cross-reaction, unrelated to the primary. I don't think the issue per se is that you are using tissue from the same patient; rather, it is that you are using a sample for which the staining characteristics are not known. For instance, if are using a lymph node section as a negative, for a stain that targets an epithelial marker (eg Her2) in the test breast sample, then your negative tissue is not appropriate, because since lymph node contains no epithelial tissue, it will not stain no matter what. Therefore if your test sample shows a positive reaction in the epithelial tissue, but for some reason that reaction is a spurious false-positive, then the lymph node negative will not reveal that. I realize that this is all very theoretical and hypothetical, but I understand the pathologist wanting to be confident in the knowledge that all potential technical issues are eliminated before making his diagnosis. Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Qu?bec, Canada J7H 1N8 tel:? 450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com ? ? ------------------------------ Message: 24 Date: Thu, 19 May 2011 09:04:24 -0700 From: "Curt Tague" Subject: [Histonet] IHC pos. & neg. control question To: Message-ID: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> Content-Type: text/plain; charset="us-ascii" I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt From rbrown <@t> mst.edu Thu May 19 12:26:24 2011 From: rbrown <@t> mst.edu (Roger Brown) Date: Thu May 19 12:26:28 2011 Subject: [Histonet] Processing sequence Message-ID: Hello Histonet community, We recently obtained a refurbished Tissue Tek VIP 2000 tissue processor and now wish to set up a few programs for processing. The operating manual lists a sequence of fluids, times, and V/P cycles etc. for one generic program. I wish ask if anyone out there might share a processing sequence that would be appropriate for processing rat whole skin samples? One item I am particularly curious about is with which steps (other than wax) should the V/P cycles be used. Thank you in advance for reply. Roger Brown Professor of Biological Sciences Missouri University of Science and Technology Room G13B Schrenk Hall 400 W. 11th St. Rolla, MO 65409-1120 E-mail: rbrown@mst.edu Phone: (573) 341-4860 Fax: (573) 341-4821 From gu.lang <@t> gmx.at Thu May 19 12:46:52 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu May 19 12:47:02 2011 Subject: AW: [Histonet] IHC pos. & neg. control question In-Reply-To: References: <201105191632.p4JGWS8w032657@gateway7.lastspam.com> Message-ID: I think this approach mixes up antibody-validation and analysis. The pathologist should be confident, that the used antibody is validated with several types of tissue and that the special antibody stains positive epitopes positive and negative epitopes negative. But the point is, that the patient tissue is unknown and shows perhaps characteristics, that lead to unspecific staining. And this has to be compared to the specific staining in the sample. Gudrun Lang -----Ursprüngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jean-Martin Lapointe Gesendet: Donnerstag, 19. Mai 2011 19:26 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] IHC pos. & neg. control question Hi Curt, I agree with your pathologist. The section that you use as a negative control (without primary) in an IHC run should ideally be tissue that is a known positive, and of the same nature as the test sample. Therefore the best sample is often a section of your positive control tissue. The purpose of this negative control is to make sure that any positive staining observed in the test sample is not due to a spurious cross-reaction, unrelated to the primary. I don't think the issue per se is that you are using tissue from the same patient; rather, it is that you are using a sample for which the staining characteristics are not known. For instance, if are using a lymph node section as a negative, for a stain that targets an epithelial marker (eg Her2) in the test breast sample, then your negative tissue is not appropriate, because since lymph node contains no epithelial tissue, it will not stain no matter what. Therefore if your test sample shows a positive reaction in the epithelial tissue, but for some reason that reaction is a spurious false-positive, then the lymph node negative will not reveal that. I realize that this is all very theoretical and hypothetical, but I understand the pathologist wanting to be confident in the knowledge that all potential technical issues are eliminated before making his diagnosis. Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Québec, Canada J7H 1N8 tel:  450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com     ------------------------------ Message: 24 Date: Thu, 19 May 2011 09:04:24 -0700 From: "Curt Tague" Subject: [Histonet] IHC pos. & neg. control question To: Message-ID: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> Content-Type: text/plain; charset="us-ascii" I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 19 13:22:41 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 19 13:22:45 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> References: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> Message-ID: <197658.64227.qm@web65715.mail.ac4.yahoo.com> The ideal situation is as follows: a known (+) control with the patient's tissue to make sure that the reaction worked, and a (-) using a section from the patient's tissue to rule out any false (+). Ren? J. From: Curt Tague To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 19, 2011 12:04 PM Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control.? The patient's own tissue cannot be used as a negative control.? The tissue that stained positively must serve as the negative control without the antibody.? This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 19 13:23:54 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 19 13:23:59 2011 Subject: [Histonet] FW: How to remove DAB to restain with DAB In-Reply-To: References: Message-ID: <173933.48486.qm@web65710.mail.ac4.yahoo.com> DAB reaction is almost permanent and extremely difficult to destain. Ren? J. From: "Margaryan, Naira" To: "histonet-request@lists.utsouthwestern.edu" Cc: "histonet@lists.utsouthwestern.edu" Sent: Thursday, May 19, 2011 12:55 PM Subject: [Histonet] FW: How to remove DAB to restain with DAB Hello, I would like to repeat my DAB staining on the same slide with DAB I run before. I know that "acid alcohol" will remove hematoxylin but how to remove DAB? I appreciate to any suggestion. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Thu May 19 13:32:10 2011 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu May 19 13:32:15 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: References: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> Message-ID: IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu May 19 13:37:38 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu May 19 13:37:43 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: References: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> Message-ID: I basically agree with you Glen. I think some people are mixing up Negative Reagent Controls (substituting negative serum, Ab diluent, etc. for antibody) and Negative Tissue Controls (substituting a tissue known to be negative for the antibody being run). It CAN be confusing. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mary.helie <@t> yale.edu Thu May 19 13:53:57 2011 From: mary.helie <@t> yale.edu (Mary Helie) Date: Thu May 19 13:54:00 2011 Subject: [Histonet] neg control Message-ID: <4DD56745.3030301@yale.edu> What??? News to me as well. From sgoebel <@t> mirnarx.com Thu May 19 14:01:45 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Thu May 19 14:01:49 2011 Subject: [Histonet] Slides for IHC Message-ID: So I just cut a bunch of slides for Ki-7...I am not going to be able to stain them until Monday. I know the best thing is to put them in 4 degrees, but will it hurt them to stay at room temp for 3 days? Putting them in the fridge gets a lot of moisture under them and they tend to lift more. Any other suggestions...the tissue is getting low in the block, and I don't want to recut them Monday. Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From LSebree <@t> uwhealth.org Thu May 19 14:07:53 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu May 19 14:07:58 2011 Subject: [Histonet] Slides for IHC In-Reply-To: References: Message-ID: I would air dry them well today then store them in a sealed container, i.e. ziploc bag, slide box, in the freezer (preferable) or fridge til Monday. They should be fine especially if you store them sealed up tight. We keep our tonsil controls for Ki67 in slide boxes in the freezer all the time with no problem. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Thursday, May 19, 2011 2:02 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides for IHC So I just cut a bunch of slides for Ki-7...I am not going to be able to stain them until Monday. I know the best thing is to put them in 4 degrees, but will it hurt them to stay at room temp for 3 days? Putting them in the fridge gets a lot of moisture under them and they tend to lift more. Any other suggestions...the tissue is getting low in the block, and I don't want to recut them Monday. Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Thu May 19 14:10:53 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa) Date: Thu May 19 14:10:54 2011 Subject: [Histonet] Histology Supervisor Needed in Fort Myers Message-ID: Position Title: Histology Supervisor Reports To: Laboratory Director Shift: Monday-Friday, 8am-5pm Location: Dermatology Pathology Laboratory for well established busy office in Fort Myers, FL founded in 2000. Requirements: * ASCP certification required * Experience leading a team of 3+ laboratory staff memebers * Experience and knowledge of OSHA, CLIA, AHCA regulations * The ability to implement and maintain QA/QC policies and procedures * Oversee safety and training * Understanding of the different lab licensing and ranges of testing Summary: * Responsible for 2 other employees within the laboratory * Work with the Director of Compliance on compliancy of the lab * Handles all laboratory compliance * Occasionally help with day to day routine histology Benefits: Health Insurance, Dental Insurance, Vision Insurance. Group Life, Short Term Disability and Long Term Disability paid by employer. Voluntary Life for self, spouse, and child. Discount on employer products and services. PTO for vacation, personal time, sick etc. Paid Holidays (7): New Years Day, Memorial Day, Fourth of July, Labor Day, Thanksgiving Day, Day after Thanksgiving Day, and Christmas Day. Bereavement Leave. Bi-annual bonuses, performance reviews, uniform and name badge. Direct Depost & 401K with employer contribution. To apply: Please send resume and salary expectations to Alyssa@alliedsearchpartners.com . At that time we will contact you to conduct a phone screen. Thank you! Alyssa Peterson, Candidate Recruitment LinkedIn: http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From rjbuesa <@t> yahoo.com Thu May 19 14:14:39 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 19 14:14:42 2011 Subject: [Histonet] Slides for IHC In-Reply-To: References: Message-ID: <542886.24031.qm@web65704.mail.ac4.yahoo.com> They will be safe at RT Ren? J. From: "sgoebel@mirnarx.com" To: Histonet@lists.utsouthwestern.edu Sent: Thursday, May 19, 2011 3:01 PM Subject: [Histonet] Slides for IHC So I just cut a bunch of slides for Ki-7...I am not going to be able to stain them until Monday.? I know the best thing is to put them in 4 degrees, but will it hurt them to stay at room temp for 3 days?? Putting them in the fridge gets a lot of moisture under them and they tend to lift more.? Any other suggestions...the tissue is getting low in the block, and I don't want to recut them Monday. Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Thu May 19 14:17:02 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu May 19 14:17:05 2011 Subject: [Histonet] IHC pos. & neg. control question References: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> Message-ID: <755A9552B75D4A078C8CD61AFA379AE6@auxs.umn.edu> I agree 100% with Glen. Jan Shivers UMN VDL ----- Original Message ----- From: "Dawson, Glen" To: Sent: Thursday, May 19, 2011 1:32 PM Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mikoff <@t> pacbell.net Thu May 19 14:26:32 2011 From: mikoff <@t> pacbell.net (Keith Mikoff) Date: Thu May 19 14:26:35 2011 Subject: [Histonet] Re: Histonet Digest, Vol 90, Issue 22 2. FW: How to remove DAB to restain with DAB (Margaryan, Naira) In-Reply-To: <201105191701.p4JH1wvB027368@nlpi155.prodigy.net> References: <201105191701.p4JH1wvB027368@nlpi155.prodigy.net> Message-ID: <6554.55298.qm@web80606.mail.mud.yahoo.com> Hi Naira, The short answer is no, it can't be done.? Unless there is a way to release the antibody from the binding site without damaging the binding site, it can't be done.? That said, somehow it?should be possible?to find a reproduce-able fix for this kind of issue, by either mending the binding site, or enhancing the activation of the?already applied antibody complex and/or??chromogen.? Some really cool tricks if practical or possible. It seems like it should, maybe with the application of an mild acidic or basic wash... with just the right reagent, the right?amount, right application?and at the right?pH.? Something to think about. Keith M. Mikoff, HTL (ASCP) ------------------------------ Message: 2 Date: Thu, 19 May 2011 11:55:14 -0500 From: "Margaryan, Naira" Subject: [Histonet] FW: How to remove DAB to restain with DAB To: "histonet-request@lists.utsouthwestern.edu" ??? Cc: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? ??? Content-Type: text/plain; charset="us-ascii" Hello, I would like to repeat my DAB staining on the same slide with DAB I run before. I know that "acid alcohol" will remove hematoxylin but how to remove DAB? I appreciate to any suggestion. Thanks in advance, Naira ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 90, Issue 22 **************************************** From mward <@t> wfubmc.edu Thu May 19 14:26:47 2011 From: mward <@t> wfubmc.edu (Martha Ward) Date: Thu May 19 14:27:01 2011 Subject: [Histonet] cost to produce one block Message-ID: I am posting this question for a co-worker. She is attempting to calculate the cost of producing one formalin-fixed, paraffin embedded block. She has the amount of personnel time it takes but is having trouble with the reagents, etc. While we know there are lots of variables she is wondering if anyone would be willing to share this information with her. She is getting differing numbers and is trying to figure out which amount is most correct. Any responses will be kept confidential. Thanks! Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 From Pete.Pedersen <@t> HealthONEcares.com Thu May 19 14:31:11 2011 From: Pete.Pedersen <@t> HealthONEcares.com (Pete.Pedersen@HealthONEcares.com) Date: Thu May 19 14:31:17 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: References: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> Message-ID: Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 19 14:36:52 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 19 14:36:56 2011 Subject: [Histonet] cost to produce one block In-Reply-To: References: Message-ID: <645466.62523.qm@web65709.mail.ac4.yahoo.com> Under separate cover I am sending an article on the subject of direct costs. Ren? J. From: Martha Ward To: Histonet Sent: Thursday, May 19, 2011 3:26 PM Subject: [Histonet] cost to produce one block I am posting this question for a co-worker.? She is attempting to calculate the cost of producing one formalin-fixed, paraffin embedded block.? She has the amount of personnel time it takes but is having trouble with the reagents, etc.? While we know there are lots of variables she is wondering if anyone would be willing to share this information with her.? She is getting differing numbers and is trying to figure out which amount is most correct.? ? Any responses will be kept confidential. Thanks! Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Thu May 19 14:39:25 2011 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu May 19 14:39:30 2011 Subject: [Histonet] IHC pos. & neg. control question References: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> Message-ID: <90354A475B420441B2A0396E5008D49692C02C@copc-sbs.COPC.local> Pete, When you run a positive control. The tissue is already a known positive (or it should be) for whichever antibody you are running regardless of prior handling. It would be impossible for this not to be so. However, with a negative, the concern is seeing how the patient tissue turns out when subjected to all the same conditions, minus the antibody. tj -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pete.Pedersen@HealthONEcares.com Sent: Thursday, May 19, 2011 12:31 PM To: GDawson@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jm.lapointe <@t> accellab.com Thu May 19 15:14:36 2011 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Thu May 19 15:14:42 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: <201105191943.p4JJhAL8003383@gateway5.lastspam.com> References: <201105191943.p4JJhAL8003383@gateway5.lastspam.com> Message-ID: I think that this is indeed what is happening here, that there is confusion between a negative control stain (positive tissue, stained without the primary ab) and a negative control tissue (tissue known to not express the marker, stained normally). I had assumed we were talking about the former, because this is what the pathologist cited by Curt wrote in his email. While a known negative control tissue is useful in an IHC run, I am somewhat alarmed to gather from the responses sent that a section without primary is NOT being included in standard IHC runs at your respective labs. To me, this is an absolute necessity in order to properly evaluate IHC results. But that might be because I work in research rather than in a clinical setting, where i'm assuming that priorities are different. Also, I do not agree with Glen's post, for the reasons outlined by Pete. Jean-Martin ------------------------------ Message: 11 Date: Thu, 19 May 2011 13:37:38 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] IHC pos. & neg. control question To: "Dawson, Glen" , Message-ID: Content-Type: text/plain; charset="us-ascii" I basically agree with you Glen. I think some people are mixing up Negative Reagent Controls (substituting negative serum, Ab diluent, etc. for antibody) and Negative Tissue Controls (substituting a tissue known to be negative for the antibody being run). It CAN be confusing. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Thu, 19 May 2011 14:53:57 -0400 From: Mary Helie Subject: [Histonet] neg control To: histonet@lists.utsouthwestern.edu Message-ID: <4DD56745.3030301@yale.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed What??? News to me as well. ------------------------------ Message: 13 Date: Thu, 19 May 2011 14:01:45 -0500 From: Subject: [Histonet] Slides for IHC To: Message-ID: Content-Type: text/plain; charset="us-ascii" So I just cut a bunch of slides for Ki-7...I am not going to be able to stain them until Monday. I know the best thing is to put them in 4 degrees, but will it hurt them to stay at room temp for 3 days? Putting them in the fridge gets a lot of moisture under them and they tend to lift more. Any other suggestions...the tissue is getting low in the block, and I don't want to recut them Monday. Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ------------------------------ Message: 14 Date: Thu, 19 May 2011 14:07:53 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] Slides for IHC To: , Message-ID: Content-Type: text/plain; charset="us-ascii" I would air dry them well today then store them in a sealed container, i.e. ziploc bag, slide box, in the freezer (preferable) or fridge til Monday. They should be fine especially if you store them sealed up tight. We keep our tonsil controls for Ki67 in slide boxes in the freezer all the time with no problem. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Thursday, May 19, 2011 2:02 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides for IHC So I just cut a bunch of slides for Ki-7...I am not going to be able to stain them until Monday. I know the best thing is to put them in 4 degrees, but will it hurt them to stay at room temp for 3 days? Putting them in the fridge gets a lot of moisture under them and they tend to lift more. Any other suggestions...the tissue is getting low in the block, and I don't want to recut them Monday. Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 19 May 2011 15:10:53 -0400 From: "Alyssa" Subject: [Histonet] Histology Supervisor Needed in Fort Myers To: Message-ID: Content-Type: text/plain; charset="us-ascii" Position Title: Histology Supervisor Reports To: Laboratory Director Shift: Monday-Friday, 8am-5pm Location: Dermatology Pathology Laboratory for well established busy office in Fort Myers, FL founded in 2000. Requirements: * ASCP certification required * Experience leading a team of 3+ laboratory staff memebers * Experience and knowledge of OSHA, CLIA, AHCA regulations * The ability to implement and maintain QA/QC policies and procedures * Oversee safety and training * Understanding of the different lab licensing and ranges of testing Summary: * Responsible for 2 other employees within the laboratory * Work with the Director of Compliance on compliancy of the lab * Handles all laboratory compliance * Occasionally help with day to day routine histology Benefits: Health Insurance, Dental Insurance, Vision Insurance. Group Life, Short Term Disability and Long Term Disability paid by employer. Voluntary Life for self, spouse, and child. Discount on employer products and services. PTO for vacation, personal time, sick etc. Paid Holidays (7): New Years Day, Memorial Day, Fourth of July, Labor Day, Thanksgiving Day, Day after Thanksgiving Day, and Christmas Day. Bereavement Leave. Bi-annual bonuses, performance reviews, uniform and name badge. Direct Depost & 401K with employer contribution. To apply: Please send resume and salary expectations to Alyssa@alliedsearchpartners.com . At that time we will contact you to conduct a phone screen. Thank you! Alyssa Peterson, Candidate Recruitment LinkedIn: http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ------------------------------ Message: 16 Date: Thu, 19 May 2011 12:14:39 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Slides for IHC To: "sgoebel@mirnarx.com" , "Histonet@lists.utsouthwestern.edu" Message-ID: <542886.24031.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 They will be safe at RT Ren? J. From: "sgoebel@mirnarx.com" To: Histonet@lists.utsouthwestern.edu Sent: Thursday, May 19, 2011 3:01 PM Subject: [Histonet] Slides for IHC So I just cut a bunch of slides for Ki-7...I am not going to be able to stain them until Monday.? I know the best thing is to put them in 4 degrees, but will it hurt them to stay at room temp for 3 days?? Putting them in the fridge gets a lot of moisture under them and they tend to lift more.? Any other suggestions...the tissue is getting low in the block, and I don't want to recut them Monday. Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 19 May 2011 14:17:02 -0500 From: "Jan Shivers" Subject: Re: [Histonet] IHC pos. & neg. control question To: "Dawson, Glen" , Message-ID: <755A9552B75D4A078C8CD61AFA379AE6@auxs.umn.edu> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I agree 100% with Glen. Jan Shivers UMN VDL ----- Original Message ----- From: "Dawson, Glen" To: Sent: Thursday, May 19, 2011 1:32 PM Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Thu, 19 May 2011 12:26:32 -0700 (PDT) From: Keith Mikoff Subject: [Histonet] Re: Histonet Digest, Vol 90, Issue 22 2. FW: How to remove DAB to restain with DAB (Margaryan, Naira) To: histonet@lists.utsouthwestern.edu Message-ID: <6554.55298.qm@web80606.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Naira, The short answer is no, it can't be done.? Unless there is a way to release the antibody from the binding site without damaging the binding site, it can't be done.? That said, somehow it?should be possible?to find a reproduce-able fix for this kind of issue, by either mending the binding site, or enhancing the activation of the?already applied antibody complex and/or??chromogen.? Some really cool tricks if practical or possible. It seems like it should, maybe with the application of an mild acidic or basic wash... with just the right reagent, the right?amount, right application?and at the right?pH.? Something to think about. Keith M. Mikoff, HTL (ASCP) ------------------------------ Message: 2 Date: Thu, 19 May 2011 11:55:14 -0500 From: "Margaryan, Naira" Subject: [Histonet] FW: How to remove DAB to restain with DAB To: "histonet-request@lists.utsouthwestern.edu" ??? Cc: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? ??? Content-Type: text/plain; charset="us-ascii" Hello, I would like to repeat my DAB staining on the same slide with DAB I run before. I know that "acid alcohol" will remove hematoxylin but how to remove DAB? I appreciate to any suggestion. Thanks in advance, Naira ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 90, Issue 22 **************************************** ------------------------------ Message: 19 Date: Thu, 19 May 2011 19:26:47 +0000 From: Martha Ward Subject: [Histonet] cost to produce one block To: Histonet Message-ID: Content-Type: text/plain; charset="us-ascii" I am posting this question for a co-worker. She is attempting to calculate the cost of producing one formalin-fixed, paraffin embedded block. She has the amount of personnel time it takes but is having trouble with the reagents, etc. While we know there are lots of variables she is wondering if anyone would be willing to share this information with her. She is getting differing numbers and is trying to figure out which amount is most correct. Any responses will be kept confidential. Thanks! Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ------------------------------ Message: 20 Date: Thu, 19 May 2011 14:31:11 -0500 From: Subject: RE: [Histonet] IHC pos. & neg. control question To: , Message-ID: Content-Type: text/plain; charset="us-ascii" Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Thu, 19 May 2011 12:36:52 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] cost to produce one block To: Martha Ward , Histonet Message-ID: <645466.62523.qm@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Under separate cover I am sending an article on the subject of direct costs. Ren? J. From: Martha Ward To: Histonet Sent: Thursday, May 19, 2011 3:26 PM Subject: [Histonet] cost to produce one block I am posting this question for a co-worker.? She is attempting to calculate the cost of producing one formalin-fixed, paraffin embedded block.? She has the amount of personnel time it takes but is having trouble with the reagents, etc.? While we know there are lots of variables she is wondering if anyone would be willing to share this information with her.? She is getting differing numbers and is trying to figure out which amount is most correct.? ? Any responses will be kept confidential. Thanks! Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Thu, 19 May 2011 12:39:25 -0700 From: "Thomas Jasper" Subject: RE: [Histonet] IHC pos. & neg. control question To: , Message-ID: <90354A475B420441B2A0396E5008D49692C02C@copc-sbs.COPC.local> Content-Type: text/plain; charset="us-ascii" Pete, When you run a positive control. The tissue is already a known positive (or it should be) for whichever antibody you are running regardless of prior handling. It would be impossible for this not to be so. However, with a negative, the concern is seeing how the patient tissue turns out when subjected to all the same conditions, minus the antibody. tj -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pete.Pedersen@HealthONEcares.com Sent: Thursday, May 19, 2011 12:31 PM To: GDawson@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 90, Issue 23 **************************************** From christiegowan <@t> msn.com Thu May 19 15:40:11 2011 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Thu May 19 15:40:14 2011 Subject: [Histonet] cost to produce one block In-Reply-To: References: Message-ID: Hi Martha, She is getting different responses because institutions pay different amounts for their consumables depending on the quantity they use and any vendor agreements they may have. I broke down my cost by utilizing a years worth of purchasing information and number of blocks produced for that year. The information included all the consumables used to produce a paraffin block as well as labor. We actually broke down the cost for everything from grossing to glass slide for each CPT code. Of course one does not need to use a whole years worth of data but that is how I did it. I would be happy to help her if she wants to talk about it off line. Thanks. Christie Gowan UAB Hospital 205.934.4991 cgowan@uabmc.edu > From: mward@wfubmc.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu, 19 May 2011 19:26:47 +0000 > Subject: [Histonet] cost to produce one block > > I am posting this question for a co-worker. She is attempting to calculate the cost of producing one formalin-fixed, paraffin embedded block. She has the amount of personnel time it takes but is having trouble with the reagents, etc. While we know there are lots of variables she is wondering if anyone would be willing to share this information with her. She is getting differing numbers and is trying to figure out which amount is most correct. Any responses will be kept confidential. > > Thanks! > > > Martha Ward, MT (ASCP) QIHC > Assistant Manager > Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center > Winston-Salem, NC 27157 > 336-716-2104 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jm.lapointe <@t> accellab.com Thu May 19 16:50:04 2011 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Thu May 19 16:50:08 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: <4DD54A4B.2B7F.00C9.1@geisinger.edu> References: <201105191943.p4JJhAL8003383@gateway5.lastspam.com> <4DD54A4B.2B7F.00C9.1@geisinger.edu> Message-ID: I can certainly agree with that. Whether it?s the patient?s sample or the known positive control that are stained without primary, either would fulfill the purpose of detecting non-specific positive staining. Jean-Martin From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: Thursday, May 19, 2011 4:50 PM To: Jean-Martin Lapointe; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC pos. & neg. control question I agree with the majority. A patient slide stained without primary antibody, and a patient section with primary antibody and a known positive control tissue, on the same slide when possible, is sufficient. I do agree, however, that the use of a negative tissue control is important in your initial antibody optimization and validation. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 email. Thank you. From Pete.Pedersen <@t> HealthONEcares.com Thu May 19 16:54:06 2011 From: Pete.Pedersen <@t> HealthONEcares.com (Pete.Pedersen@HealthONEcares.com) Date: Thu May 19 16:54:13 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: <90354A475B420441B2A0396E5008D49692C02C@copc-sbs.COPC.local> References: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> <90354A475B420441B2A0396E5008D49692C02C@copc-sbs.COPC.local> Message-ID: Thomas, Agreed, however, how can you say with certainty that the control is still good, or the antibody is still performing optimally? Hypothetically speaking, if you had a known positive control and ran it like a patient specimen (positive and negative) and had staining in the negatively stained control that you had been only running as a positively stained control prior, how would you proceed? What good is a positive control without if it is not treated identically as patient tissue. If you had none specific staining in a patient negative but is was also there in your known positive control which you stained negatively as well, then you could mark up to nonspecific staining to reagent or IHC user error. If the negatively stained positive control stains truly negative and the patient negative has nonspecific staining then you would know patient tissue is compromised or has been mistreated somewhere along the way because your positively stained and negatively stained positive controls demonstrate the staining was done correctly, correct? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -----Original Message----- From: Thomas Jasper [mailto:tjasper@copc.net] Sent: Thursday, May 19, 2011 1:39 PM To: Pedersen Pete; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Pete, When you run a positive control. The tissue is already a known positive (or it should be) for whichever antibody you are running regardless of prior handling. It would be impossible for this not to be so. However, with a negative, the concern is seeing how the patient tissue turns out when subjected to all the same conditions, minus the antibody. tj -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pete.Pedersen@HealthONEcares.com Sent: Thursday, May 19, 2011 12:31 PM To: GDawson@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Thu May 19 17:12:40 2011 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu May 19 17:12:49 2011 Subject: [Histonet] IHC pos. & neg. control question References: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> <90354A475B420441B2A0396E5008D49692C02C@copc-sbs.COPC.local> Message-ID: <90354A475B420441B2A0396E5008D49692C02D@copc-sbs.COPC.local> Pete, Can't argue with that. I think for the sake of expediency most clinical services run a "known" positive and a patient slide for negative. In the case of H. Pylori, for instance, we may cut a box of control slides and it's possible to go through the area where the organisms were. This also happens with controls that demonstrate positivity by other means epithelium, tumor, etc. We may have to re-run tests in these situations. I believe we are similar to many clinical labs in our reliance on known positives. tj -----Original Message----- From: Pete.Pedersen@HealthONEcares.com [mailto:Pete.Pedersen@HealthONEcares.com] Sent: Thursday, May 19, 2011 2:54 PM To: Thomas Jasper; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Thomas, Agreed, however, how can you say with certainty that the control is still good, or the antibody is still performing optimally? Hypothetically speaking, if you had a known positive control and ran it like a patient specimen (positive and negative) and had staining in the negatively stained control that you had been only running as a positively stained control prior, how would you proceed? What good is a positive control without if it is not treated identically as patient tissue. If you had none specific staining in a patient negative but is was also there in your known positive control which you stained negatively as well, then you could mark up to nonspecific staining to reagent or IHC user error. If the negatively stained positive control stains truly negative and the patient negative has nonspecific staining then you would know patient tissue is compromised or has been mistreated somewhere along the way because your positively stained and negatively stained positive controls demonstrate the staining was done correctly, correct? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -----Original Message----- From: Thomas Jasper [mailto:tjasper@copc.net] Sent: Thursday, May 19, 2011 1:39 PM To: Pedersen Pete; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Pete, When you run a positive control. The tissue is already a known positive (or it should be) for whichever antibody you are running regardless of prior handling. It would be impossible for this not to be so. However, with a negative, the concern is seeing how the patient tissue turns out when subjected to all the same conditions, minus the antibody. tj -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pete.Pedersen@HealthONEcares.com Sent: Thursday, May 19, 2011 12:31 PM To: GDawson@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu May 19 18:13:12 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu May 19 18:13:16 2011 Subject: [Histonet] Denatured Alcohol Message-ID: <4F00BDDCE8604D4F99DF9A3352044111@JoePC> Greetings and Salutations Histoland, I have a question about denatured alcohol. I work in a government facility and absolute alcohol (200 proof) is still considered a controlled substance. This requires a monthly inventory by someone from another department. Years ago (ok many, many years ago) I remember that 200 proof had an IRS sticker covering the cap. The alcohol we have is "denatured alcohol, 200 proof", which according to the MSDS is cut with kerosene. There is no IRS sticker on the bottles. Question #1- If the alcohol is cut with something other than ethanol, ( which usually it's cut with methanol) is it still 200 proof? Question #2- If it is "denatured", it is considered not suitable for drinking. If the substance is not suitable for drinking, then why would it be considered a controlled substance? See my dilemma? We would like to get it removed from our "controlled" substance list, but we need a reliable source. The company (whom shall remain nameless because of my past history of inflaming vendors) was useless. I don't think the government accepts Wikipedia as an authoritative source. Thanks JTT From Timothy.Morken <@t> ucsfmedctr.org Thu May 19 18:24:31 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu May 19 18:24:45 2011 Subject: [Histonet] Denatured Alcohol In-Reply-To: <4F00BDDCE8604D4F99DF9A3352044111@JoePC> References: <4F00BDDCE8604D4F99DF9A3352044111@JoePC> Message-ID: Joe, I thought only ethanol, punctilious (fancy word for pure), was controlled. If it is denatured it should not be controlled. The "200 proof" normally means 100%, but the if the non-ethanol portion is another alcohol, then it still applies. The key is there is no water in it. I can't say I've ever heard of it being cut with kerosene! Usually it's methanol or isopropyl alcohol. BTW, a historical tidbit: " in the 18th century and until 1 January 1980, the United Kingdom defined alcohol content in terms of "proof spirit", which was defined as the most dilute spirit that would sustain combustion of gunpowder.[1]" (from Wikipedia) The term originated in the 18th century, when payments to British sailors included rations of rum. To ensure that the rum had not been watered down, it was "proved" by dousing gunpowder in it, then tested to see if the gunpowder would ignite. If it did not, then the rum contained too much water and was considered to be "under proof". It was found that gunpowder would not burn in rum that contained less than 57.15% abv. Therefore, rum that contained this percentage of alcohol was defined to have "100 degrees proof". Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, May 19, 2011 4:13 PM To: Histonet Subject: [Histonet] Denatured Alcohol Greetings and Salutations Histoland, I have a question about denatured alcohol. I work in a government facility and absolute alcohol (200 proof) is still considered a controlled substance. This requires a monthly inventory by someone from another department. Years ago (ok many, many years ago) I remember that 200 proof had an IRS sticker covering the cap. The alcohol we have is "denatured alcohol, 200 proof", which according to the MSDS is cut with kerosene. There is no IRS sticker on the bottles. Question #1- If the alcohol is cut with something other than ethanol, ( which usually it's cut with methanol) is it still 200 proof? Question #2- If it is "denatured", it is considered not suitable for drinking. If the substance is not suitable for drinking, then why would it be considered a controlled substance? See my dilemma? We would like to get it removed from our "controlled" substance list, but we need a reliable source. The company (whom shall remain nameless because of my past history of inflaming vendors) was useless. I don't think the government accepts Wikipedia as an authoritative source. Thanks JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshea121 <@t> roadrunner.com Thu May 19 20:11:01 2011 From: jshea121 <@t> roadrunner.com (Shea's) Date: Thu May 19 20:11:05 2011 Subject: [Histonet] IHC pos. & neg. control question Message-ID: <6EFF67C813C0488CB7156BC8FF67B14D@JoannePC> Curt is right, according to CAP ... ANP.22570 QC - Antibodies Phase II Appropriate negative controls are used. NOTE: Negative controls must assess the presence of nonspecific staining in patient tissue as well as the specificity of each antibody. Results of controls must be documented, either in internal laboratory records, or in the patient report. A statement in the report such as, "All controls show appropriate reactivity" is sufficient. A negative reagent control is used to assess nonspecific or aberrant staining in patient tissue related to the antigen retrieval conditions and/or detection system used. A separate section of patient tissue is processed using the same reagent and epitope retrieval protocol as the patient test slide, except that the primary antibody is omitted, and replaced by any one of the following: ? An unrelated antibody of the same isotype as the primary antibody (for monoclonal primary antibodies) ? An unrelated antibody from the same animal species as the primary antibody (for polyclonal primary antibodies) ? The negative control reagent included in the staining kit ? The diluent/buffer solution in which the primary antibody is diluted In general, a separate negative reagent control should be run for each block of patient tissue being immunostained; however, for cases in which there is simultaneous staining of multiple blocks from the same specimen with the same antibody (e.g. cytokeratin staining of multiple axillary sentinel lymph nodes), performing a single negative control on one of the blocks may be sufficient provided that all such blocks are fixed and processed identically. This exception does not apply to stains on different types of tissues or those using different antigen retrieval protocols or antibody detection systems. The laboratory director must determine which cases will have only one negative reagent control, and this must be specified in the department's procedure manual. The negative reagent control would ideally control for each reagent protocol and antibody retrieval condition; however, large antibody panels often employ multiple antigen retrieval procedures. In such cases, a reasonable minimum control would be to perform the negative reagent control using the most aggressive retrieval procedure in the particular antibody panel. Aggressiveness of antigen retrieval (in decreasing order) is as follows: pressure cooker; enzyme digestion, boiling; microwave; steamer; water bath. High pH retrieval should be considered more aggressive than comparable retrieval in citrate buffer at pH 6.0. It is also important to assess the specificity of each antibody by a negative tissue control, which must show no staining of tissues known to lack the antigen.The negative tissue control is processed using the same fixation, epitope retrieval and immunostaining protocols as the patient tissue. Unexpected positive staining of such tissues indicates that the test has lost specificity, perhaps because of improper antibody concentration or excessive antigen retrieval. Intrinsic properties of the test tissue may also be the cause of "non-specific" staining. For example, tissues with high endogenous biotin activity such as liver or renal tubules may simulate positive staining when using a detection method based on biotin labeling. A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks.These can provide simultaneous positive and negative tissue controls, and are considered "best practice" (see below). 2. The positive control slide or patient test slides, if these slides contain tissue elements that should not react with the antibody. 3. A separate negative tissue control slide. The type of negative tissue control used (i.e. separate sections, internal controls or multitissue blocks) should be specified in the laboratory manual (refer to ANP.22250). Multitissue blocks may be considered best practice and can have a major role in maintaining quality. When used as a combined positive and negative tissue control as mentioned above, they can serve as a permanent record documenting the sensitivity and specificity of every stain, particularly when mounted on the same slide as the patient tissue. When the components are chosen appropriately, multitissue blocks may be used for many different primary antibodies, decreasing the number of different control blocks needed by the laboratory. Multitissue blocks are also ideal for determining optimal titers of primary antibodies since they allow simultaneous evaluation of many different pieces of tissue. Finally, they are a useful and efficient means to screen new antibodies for sensitivity and specificity or new lots of antibody for consistency, which should be done before putting any antibody into diagnostic use. Evidence of Compliance: ? Written procedure for the use of negative reagent and tissue controls for IHC AND ? Patient reports or worksheet with control results From AnthonyH <@t> chw.edu.au Thu May 19 21:34:37 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu May 19 21:35:11 2011 Subject: [Histonet] RE: IHC pos. & neg. control question In-Reply-To: References: <201105191632.p4JGWS8w032657@gateway7.lastspam.com> Message-ID: <6D6BD1DE8A5571489398B392A38A7157188569B3@xmdb02.nch.kids> No, the best sample for negative control is NOT a section of your positive control tissue. The positive control would have already been tested for "spurious cross-reactions". Why continue to test it? The patient's tissue has not been tested. So a mirror section is used. Does the patient's tissue have brown pigments that could be confused with DAB? Are there unique substances (of unknown type) that might bind non-specifically to the IPX reagents? Is there endogenous biotin or excessive peroxidase that might give a false positive reaction? These are the answers I expect to obtain from my negative control. I already know these do not occur on my positive control tissue. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean-Martin Lapointe Sent: Friday, 20 May 2011 3:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question Hi Curt, I agree with your pathologist. The section that you use as a negative control (without primary) in an IHC run should ideally be tissue that is a known positive, and of the same nature as the test sample. Therefore the best sample is often a section of your positive control tissue. The purpose of this negative control is to make sure that any positive staining observed in the test sample is not due to a spurious cross-reaction, unrelated to the primary. I don't think the issue per se is that you are using tissue from the same patient; rather, it is that you are using a sample for which the staining characteristics are not known. For instance, if are using a lymph node section as a negative, for a stain that targets an epithelial marker (eg Her2) in the test breast sample, then your negative tissue is not appropriate, because since lymph node contains no epithelial tissue, it will not stain no matter what. Therefore if your test sample shows a positive reaction in the epithelial tissue, but for some reason that reaction is a spurious false-positive, then the lymph node negative will not reveal that. I realize that this is all very theoretical and hypothetical, but I understand the pathologist wanting to be confident in the knowledge that all potential technical issues are eliminated before making his diagnosis. Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Qu?bec, Canada J7H 1N8 tel:? 450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com ? ? ------------------------------ Message: 24 Date: Thu, 19 May 2011 09:04:24 -0700 From: "Curt Tague" Subject: [Histonet] IHC pos. & neg. control question To: Message-ID: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> Content-Type: text/plain; charset="us-ascii" I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From AnthonyH <@t> chw.edu.au Thu May 19 21:42:22 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu May 19 21:42:41 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: References: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> Message-ID: <6D6BD1DE8A5571489398B392A38A7157188569FA@xmdb02.nch.kids> It still comes down to why test the positive control for false positive reactions after it has already been determined that they are not present. But what you say about positive controls matching patient's tissue with regards to pre-fixing, fixing and processing is correct but in the real world we cannot guarantee that this would happen. We can only work on averages and hope that conditions are as similar as possible. In a perfect world we would not need negative, nor dare I say positive, controls. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pete.Pedersen@HealthONEcares.com Sent: Friday, 20 May 2011 5:31 AM To: GDawson@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From AnthonyH <@t> chw.edu.au Thu May 19 21:47:23 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu May 19 21:49:01 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: References: <201105191943.p4JJhAL8003383@gateway5.lastspam.com> <4DD54A4B.2B7F.00C9.1@geisinger.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A715718856A2F@xmdb02.nch.kids> "either would fulfill the purpose of detecting non-specific positive staining" - NO Not in the patients sample unless it is included (which for diagnostic uses is the most important). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean-Martin Lapointe Sent: Friday, 20 May 2011 7:50 AM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question I can certainly agree with that. Whether it?s the patient?s sample or the known positive control that are stained without primary, either would fulfill the purpose of detecting non-specific positive staining. Jean-Martin From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: Thursday, May 19, 2011 4:50 PM To: Jean-Martin Lapointe; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC pos. & neg. control question I agree with the majority. A patient slide stained without primary antibody, and a patient section with primary antibody and a known positive control tissue, on the same slide when possible, is sufficient. I do agree, however, that the use of a negative tissue control is important in your initial antibody optimization and validation. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 email. Thank you. ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tora.bardal <@t> bio.ntnu.no Fri May 20 02:24:10 2011 From: tora.bardal <@t> bio.ntnu.no (Tora Bardal) Date: Fri May 20 02:24:29 2011 Subject: [Histonet] accidentally frozen samples Message-ID: <4DD6171A.1010208@bio.ntnu.no> One of our students accidentally put his samples in the freezer after formaldehyde/glutardialdehyde/PBS fixation. The samples (fish very early stage, of course rare species) were meant for LM/EM morphology studies. Good morphology is out, but is there a chance he can do cartilage/bone staining? Or do we need to send him abroad for new sampling at the next spawning time? Tora Bardal Senior Engineer NTNU Center of Fisheries and Aquaculture From louise.renton <@t> gmail.com Fri May 20 02:30:51 2011 From: louise.renton <@t> gmail.com (Louise Renton) Date: Fri May 20 02:31:06 2011 Subject: [Histonet] accidentally frozen samples In-Reply-To: <4DD6171A.1010208@bio.ntnu.no> References: <4DD6171A.1010208@bio.ntnu.no> Message-ID: Probably the bone staining will be OK - how about sending the student to the arctic circle for a few days in winter? - that may be a good lesson about not freezing tissues. Just kidding :-) On Fri, May 20, 2011 at 9:24 AM, Tora Bardal wrote: > One of our students accidentally put his samples in the freezer after > formaldehyde/glutardialdehyde/PBS fixation. The samples (fish very early > stage, of course rare species) were meant for LM/EM morphology studies. > Good morphology is out, but is there a chance he can do cartilage/bone > staining? Or do we need to send him abroad for new sampling at the next > spawning time? > > > Tora Bardal > > Senior Engineer > NTNU Center of Fisheries and Aquaculture > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From lchauss <@t> yahoo.com Fri May 20 07:14:53 2011 From: lchauss <@t> yahoo.com (Leslie Chaussey) Date: Fri May 20 07:14:58 2011 Subject: [Histonet] Histology survey In-Reply-To: <862902.95048.qm@web120803.mail.ne1.yahoo.com> References: <4AF58ED0C9421B4896A32F5B018FAD053F5BD283@NMH-PEXCSCC11.nmh.org> <862902.95048.qm@web120803.mail.ne1.yahoo.com> Message-ID: <294549.86036.qm@web120818.mail.ne1.yahoo.com> Histology Colleagues, I am interested in collecting information to better understand what motivates Histotechnologists in the workplace as well as job satisfaction levels within our field.? This ten question survey should just take a few minutes to complete and survey responses will be kept in strict confidence.? You are under no obligation to finish the survey and may stop at any time. I am currently looking at data specific to the United States within the clinical setting.? Please do not complete this survey if you live outside the United States or if you currently work in a research setting.? Here is the survey link: http://www.surveymonkey.com/s/YTX97M8 Please note:? this survey will close on May 23rd or when 100 responses are received, whichever occurs first.? If clicking on the link does not work, please copy and paste the link into your browser. I would appreciate if you would also forward this opportunity to other histologists you know who may be interested in participating.? I would be willing to share the results with whoever is interested.? Thank you for your help with this survey. Leslie Chaussey, B.S., HT (ASCP) lchauss@yahoo.com This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From GDawson <@t> dynacaremilwaukee.com Fri May 20 07:44:40 2011 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri May 20 07:44:45 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: References: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> Message-ID: Pete, OK, time for an example: A pathologist orders 4 IHC's on a block. I run 5 slides total: 4 IHC slides with a section of patient tissue and a known positive. 1 slide with patient tissue only for the negative control. The one negative control is put through the retrieval/protocol that is most likely to cause nonspecific staining. I don't run the known positive control tissue used on the 4 actual IHC slides as negative controls. Perhaps I didn't point out that my original post was addressing the negative control? I'm a bit surprised by the confusion. As for the question of "how I know the staining seen in a positive control is truly positive?": All known positive controls are tested previously so I know that they work for the antibody that I'm using them for and I would assume the pathologist uses morphology and localization of staining to determine that the positive control is working. That's what I do. I've gone through 7 CAP inspections utilizing the practices above with no problems thus far. Perhaps you could enlighten me on IHC requirements that I haven't come across. Glen D. -----Original Message----- From: Pete.Pedersen@HealthONEcares.com [mailto:Pete.Pedersen@HealthONEcares.com] Sent: Thursday, May 19, 2011 2:31 PM To: Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Fri May 20 07:45:21 2011 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri May 20 07:45:25 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: <90354A475B420441B2A0396E5008D49692C02C@copc-sbs.COPC.local> References: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> <90354A475B420441B2A0396E5008D49692C02C@copc-sbs.COPC.local> Message-ID: Tj, Amen brother! GD -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Thursday, May 19, 2011 2:39 PM To: Pete.Pedersen@HealthONEcares.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Pete, When you run a positive control. The tissue is already a known positive (or it should be) for whichever antibody you are running regardless of prior handling. It would be impossible for this not to be so. However, with a negative, the concern is seeing how the patient tissue turns out when subjected to all the same conditions, minus the antibody. tj -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pete.Pedersen@HealthONEcares.com Sent: Thursday, May 19, 2011 12:31 PM To: GDawson@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri May 20 08:09:15 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 20 08:09:19 2011 Subject: [Histonet] Denatured Alcohol In-Reply-To: <4F00BDDCE8604D4F99DF9A3352044111@JoePC> References: <4F00BDDCE8604D4F99DF9A3352044111@JoePC> Message-ID: <506239.85585.qm@web65710.mail.ac4.yahoo.com> Q#1 - yes, it is still 200 proof Q#2 - no it should be not considered as a controlled substance. If you still have to keep a log of its use = bureaucratic ignorance and lack of adaptability, like the case in the British army were the artillery kept in the personnel assigned to each?movable gun 2 soldiers to "restrain" the horses that pulled the piece, even years after there were no more horses pulling the piece. Ren? J.? From: Joe Nocito To: Histonet Sent: Thursday, May 19, 2011 7:13 PM Subject: [Histonet] Denatured Alcohol Greetings and Salutations Histoland, ? ? I have a question about denatured alcohol. I work in a government facility and absolute alcohol (200 proof) is still considered a controlled substance. This requires a monthly inventory by someone from another department.? Years ago (ok many, many years ago) I remember that 200 proof had an IRS sticker covering the cap. ? ? The alcohol we have is "denatured alcohol, 200 proof",? which according to the MSDS is cut with kerosene. There is no IRS sticker on the bottles. Question #1- If the alcohol is cut with something other than ethanol, ( which usually it's cut with methanol) is it still 200 proof? Question #2-? If it is "denatured", it is considered not suitable for drinking. If the substance is not suitable for drinking, then why would it be considered a controlled substance? See my dilemma? We would like to get it removed from our "controlled" substance list, but we need a reliable source. The company (whom shall remain nameless because of my past history of inflaming vendors) was useless. I don't think the government accepts Wikipedia as an authoritative source. Thanks JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri May 20 08:15:04 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 20 08:15:14 2011 Subject: [Histonet] accidentally frozen samples In-Reply-To: <4DD6171A.1010208@bio.ntnu.no> References: <4DD6171A.1010208@bio.ntnu.no> Message-ID: <390782.78750.qm@web65706.mail.ac4.yahoo.com> The bast approach will be to try to do the cartilage/bone staining. It is preferable to do this than to consider the samples already ruined and incur in the expense of a new sampling. Ren? J. From: Tora Bardal To: histonet@lists.utsouthwestern.edu Sent: Friday, May 20, 2011 3:24 AM Subject: [Histonet] accidentally frozen samples One of our students accidentally put his samples in the freezer after formaldehyde/glutardialdehyde/PBS fixation. The samples (fish very early stage, of course rare species) were meant for LM/EM morphology studies. Good morphology is out, but is there a chance he can do cartilage/bone staining? Or do we need to send him abroad for new sampling at the next spawning time? Tora Bardal Senior Engineer NTNU Center of Fisheries and Aquaculture _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Fri May 20 08:29:55 2011 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Fri May 20 08:30:54 2011 Subject: [Histonet] IHC pos. & neg. control question References: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> <90354A475B420441B2A0396E5008D49692C02C@copc-sbs.COPC.local> Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A6DF@UWHC-MAIL2.uwhis.hosp.wisc.edu> And the block in question has already been proven positive using THAT procedure and antibody during validation. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Thomas Jasper Sent: Thu 5/19/2011 2:39 PM To: Pete.Pedersen@HealthONEcares.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Pete, When you run a positive control. The tissue is already a known positive (or it should be) for whichever antibody you are running regardless of prior handling. It would be impossible for this not to be so. However, with a negative, the concern is seeing how the patient tissue turns out when subjected to all the same conditions, minus the antibody. tj -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pete.Pedersen@HealthONEcares.com Sent: Thursday, May 19, 2011 12:31 PM To: GDawson@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague <@t> pathologyarts.com Fri May 20 09:22:02 2011 From: c.tague <@t> pathologyarts.com (Curt Tague) Date: Fri May 20 09:22:13 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: <064F1ACBAE8A78469AE2E41D533D87E505A6DF@UWHC-MAIL2.uwhis.hosp.wisc.edu> References: <019801cc163e$6c1487d0$443d9770$@tague@pathologyarts.com> <90354A475B420441B2A0396E5008D49692C02C@copc-sbs.COPC.local> <064F1ACBAE8A78469AE2E41D533D87E505A6DF@UWHC-MAIL2.uwhis.hosp.wisc.edu> Message-ID: <01f501cc16f9$497df100$dc79d300$@tague@pathologyarts.com> All very insightful input. I took the liberty of forwarding some of the comments to the client, I think I errantly sent some of the critical comments about him too. I'll probably hear about it later, the best was the clown residency, I was in tears laughing. i'll let you all know what he says about CAPs protocol, don't exactly know how he can argue against that. Thanks all, Curt -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Friday, May 20, 2011 6:30 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question And the block in question has already been proven positive using THAT procedure and antibody during validation. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Thomas Jasper Sent: Thu 5/19/2011 2:39 PM To: Pete.Pedersen@HealthONEcares.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Pete, When you run a positive control. The tissue is already a known positive (or it should be) for whichever antibody you are running regardless of prior handling. It would be impossible for this not to be so. However, with a negative, the concern is seeing how the patient tissue turns out when subjected to all the same conditions, minus the antibody. tj -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pete.Pedersen@HealthONEcares.com Sent: Thursday, May 19, 2011 12:31 PM To: GDawson@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. & neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: "I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately." Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri May 20 11:26:32 2011 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri May 20 11:26:39 2011 Subject: [Histonet] Re: Accidental freezing of fish samples Message-ID: <000601cc170a$afff7cd0$0ffe7670$@callis@bresnan.net> You wrote: Probably the bone staining will be OK - how about sending the student to the arctic circle for a few days in winter? - that may be a good lesson about not freezing tissues. Just kidding :-) On Fri, May 20, 2011 at 9:24 AM, Tora Bardal <@t> bio.ntnu.no>wrote: > One of our students accidentally put his samples in the freezer after formaldehyde/glutaraldehyde/PBS fixation. The samples (fish very early stage, of course rare species) were meant for LM/EM morphology studies. Good morphology is out, but is there a chance > he can do cartilage/bone staining? Or do we need to send him abroad for new sampling at the next spawning time? > Tora Bardal > Senior Engineer > NTNU Center of Fisheries and Aquaculture I was amused by Louise's reply. Your comment "good morphology is out" is correct, especially for EM where bone and cartilage may NOT be optimal after a freeze/ thaw. EM requires optimal fixation and handling to have the best results. There will probably be too much freezing artifact damage (large water ice crystal formation) in the EM samples. However, if doing light microscopy and not worried about soft tissues and with paraffin processing , the bone and cartilage may be ok, but not entirely be ideal ......individual bone and cartilage cells, along with soft tissues, may still show the effects of freezing artifact. These effects may be seen more in cartilage with its higher water content, but give the LM samples a try. You may salvage part of the study. Then send the student out for samples and insist he/she reads up on and understand the damage freezing/thawing can have on fixed samples. I suggest, in lieu of sending your student to the Arctic, that he/she reads the excellent discussion of freezing artifact by Charles Scouten (Myneurolab website) , titled Tips and Techniques: Freezing Biological Samples to prevent this from happening again. A supporting publication, cited by Scouten, is Jongebloed, W.L., Stokroos, D., Kalicharan, D., and Van der Want, J.J.L. Is Cryopreservation Superior Over Tannic Acid/Arginine/Osmium Tetroxide non-Coating Preparation in Field Emission Scanning Electron Microscopy. Scanning Microscopy 13: 93-109, 1999. This paper might help clarify what damage occurs for SEM even though you might be doing TEM (?). I will be happy to send the article in a separate email if you want it. It would be interesting to have follow up on your results, so keep us posted. Good luck Gayle Callis HTL/HT/MT(ASCP) From MSHERWOOD <@t> PARTNERS.ORG Fri May 20 11:38:57 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri May 20 11:39:02 2011 Subject: [Histonet] Re: Accidental freezing of fish samples In-Reply-To: <000601cc170a$afff7cd0$0ffe7670$@callis@bresnan.net> References: <000601cc170a$afff7cd0$0ffe7670$@callis@bresnan.net> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB575E@PHSXMB30.partners.org> Gayle, I would be interested in the articles as well. I had a similar experience: nerve samples from rats were being shipped to me in K2 (which I had previously sent to them). I specifically said not to freeze the samples; they shipped them on dry ice! I was just doing one micron sectioning, but they were useless. Thanks! Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Friday, May 20, 2011 12:27 PM To: 'Histonet' Subject: [Histonet] Re: Accidental freezing of fish samples You wrote: Probably the bone staining will be OK - how about sending the student to the arctic circle for a few days in winter? - that may be a good lesson about not freezing tissues. Just kidding :-) On Fri, May 20, 2011 at 9:24 AM, Tora Bardal <@t> bio.ntnu.no>wrote: > One of our students accidentally put his samples in the freezer after formaldehyde/glutaraldehyde/PBS fixation. The samples (fish very early stage, of course rare species) were meant for LM/EM morphology studies. Good morphology is out, but is there a chance > he can do cartilage/bone staining? Or do we need to send him abroad for new sampling at the next spawning time? > Tora Bardal > Senior Engineer > NTNU Center of Fisheries and Aquaculture I was amused by Louise's reply. Your comment "good morphology is out" is correct, especially for EM where bone and cartilage may NOT be optimal after a freeze/ thaw. EM requires optimal fixation and handling to have the best results. There will probably be too much freezing artifact damage (large water ice crystal formation) in the EM samples. However, if doing light microscopy and not worried about soft tissues and with paraffin processing , the bone and cartilage may be ok, but not entirely be ideal ......individual bone and cartilage cells, along with soft tissues, may still show the effects of freezing artifact. These effects may be seen more in cartilage with its higher water content, but give the LM samples a try. You may salvage part of the study. Then send the student out for samples and insist he/she reads up on and understand the damage freezing/thawing can have on fixed samples. I suggest, in lieu of sending your student to the Arctic, that he/she reads the excellent discussion of freezing artifact by Charles Scouten (Myneurolab website) , titled Tips and Techniques: Freezing Biological Samples to prevent this from happening again. A supporting publication, cited by Scouten, is Jongebloed, W.L., Stokroos, D., Kalicharan, D., and Van der Want, J.J.L. Is Cryopreservation Superior Over Tannic Acid/Arginine/Osmium Tetroxide non-Coating Preparation in Field Emission Scanning Electron Microscopy. Scanning Microscopy 13: 93-109, 1999. This paper might help clarify what damage occurs for SEM even though you might be doing TEM (?). I will be happy to send the article in a separate email if you want it. It would be interesting to have follow up on your results, so keep us posted. Good luck Gayle Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From mhale <@t> carisls.com Fri May 20 13:46:12 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Fri May 20 13:47:20 2011 Subject: [Histonet] Las Vegas HT Position Message-ID: <6F33D8418806044682A391273399860F0831F952@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! We are a 5 physician gastroenterology practice located in Las Vegas, NV looking for a certified HT or HTL. Candidate must meet CLIA grossing requirements. The candidate will be responsible for routine histology duties. This is a full-time position that offers a competitive salary and flexible hours. Interested applicants should contact Meredith Hale, phone (214)596-2219 or e-mail mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 214-596-7095 mhale@carisls.com From mhale <@t> carisls.com Fri May 20 13:48:16 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Fri May 20 13:49:24 2011 Subject: [Histonet] Recall: Las Vegas HT Position Message-ID: <6F33D8418806044682A391273399860F0831F95D@s-irv-ex301.PathologyPartners.intranet> Hale, Meredith would like to recall the message, "Las Vegas HT Position". From mhale <@t> carisls.com Fri May 20 13:50:04 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Fri May 20 13:51:13 2011 Subject: [Histonet] Las Vegas HT Position Message-ID: <6F33D8418806044682A391273399860F0831F965@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! We are a 5 physician gastroenterology practice located in Las Vegas, NV looking for a certified HT or HTL. Candidate must meet CLIA grossing requirements. The candidate will be responsible for routine histology duties. This is a part time position that offers a competitive salary and flexible hours. Interested applicants should contact Meredith Hale, phone (214)596-2219 or e-mail mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 214-596-7095 mhale@carisls.com From jaylundgren <@t> gmail.com Fri May 20 13:57:09 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri May 20 13:57:15 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: <8708745368093858333@unknownmsgid> References: <90354A475B420441B2A0396E5008D49692C02C@copc-sbs.COPC.local> <064F1ACBAE8A78469AE2E41D533D87E505A6DF@UWHC-MAIL2.uwhis.hosp.wisc.edu> <8708745368093858333@unknownmsgid> Message-ID: Curt, Thank you. I'm here all week, try the veal. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) From c.tague <@t> pathologyarts.com Fri May 20 14:45:58 2011 From: c.tague <@t> pathologyarts.com (Curt Tague) Date: Fri May 20 14:46:08 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: References: <90354A475B420441B2A0396E5008D49692C02C@copc-sbs.COPC.local> <064F1ACBAE8A78469AE2E41D533D87E505A6DF@UWHC-MAIL2.uwhis.hosp.wisc.edu> <8708745368093858333@unknownmsgid> Message-ID: <020801cc1726$8a40a430$9ec1ec90$@tague@pathologyarts.com> "If an inspector saw that, you would be on immediate suspension." This was the last statement from the original post, I chose not to include it initially but it's just too juicy to keep from everyone else. Having forwarded several of the responses I think he has come to understand that it's not necessarily a requirement that will have us on immediate suspension but rather a preference that, being a private lab, I will gladly provide to keep the business. Thanks again everyone and have a good weekend. Curt From: Jay Lundgren [mailto:jaylundgren@gmail.com] Sent: Friday, May 20, 2011 11:57 AM To: Curt Tague Cc: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC pos. & neg. control question Curt, Thank you. I'm here all week, try the veal. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) From dmccaig <@t> ckha.on.ca Fri May 20 14:48:05 2011 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri May 20 14:48:11 2011 Subject: [Histonet] SMM-HC Message-ID: We are using Biocare Smooth Muscle Myosin-Heavy Chains (AP Protocol using Warp Red and previously Vulcan Fast Red)on the Nemesis auto stainer.. Lately we have notice considerable background staining. Any suggestions. The slides almost look like in the old days when you put too much albumin ( as an adhesive) on a slide and did an H&E and the eosin coated the slide. We do not notice this with other antibodies. Any suggestions? Diana From amosbrooks <@t> gmail.com Fri May 20 14:55:17 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri May 20 14:55:21 2011 Subject: [Histonet] BrdU on rat tissue Message-ID: Hi, BrdU from Sigma cat # B2531 works really well. My primary application is in mice, however the target for this antibody is the BrdU that was incorporated into the DNA strand not the animal itself so you should be able to use it in ANY animal. (Don't forget to denature). Good Luck, Amos Message: 2 Date: Thu, 19 May 2011 12:08:38 -0500 From: "Michele Wich" Subject: [Histonet] BrdU on rat tissue To: Message-ID: < 62A8156F8071C8439080D626DF8C33A6017A9D04@wave-mail.7thwave.local> Content-Type: text/plain; charset="us-ascii" Can anyone recommend a good commercially available kit for BrdU on rat tissue? Thanks in advance for any suggestions. From amosbrooks <@t> gmail.com Fri May 20 15:15:34 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri May 20 15:15:39 2011 Subject: [Histonet] IHC pos. & neg. control question Message-ID: Hi, This is simply a question of definitions. They are actually both right. If you have the patient tissue as a negative control you are not using the primary antibody on it but are replacing it (ideally) with an Ig with the same isotype AND dilution (universal negative is stupid). So if your primary is an IgG1 from a mouse and you use it at 1:100, your negative control would be a non-immune mouse serum IgG1 diluted to 1:100. Running this on unrelated material will not show you anything in this case. what it will show you is that the detection system you are using is specific to the antibody you put on the tissue and not to A) something else in the tissue or B) something on the Ig that is not the epitope itself. Although, If you run the same primary antibody on tissue that you expect will not react with the primary antibody, this would prove that the antibody reaction is specific to the antigenin question. This does raise the question of what to do about ubiquitous antigens (like ubiquitin) that are actually everywhere. Every cell has a cell cycle so they will all at one point or another show proliferation or degeneration for example. Does this mean you don't run a negative control? No you need to look at it in the perspective of expected results. There are various ways of using negative (and positive) controls. It would actually be cost prohibitive to run ALL the possible positive and negative controls for every test that we as histologists do. We need to discuss with our pathologists what they want to have done to give them the confidence in our testing to support their diagnosis. But remember that just because another lab and another pathologist does it differently doesn't mean it is wrong. It's just different. Happy Friday Folks, Amos Message: 5 Date: Thu, 19 May 2011 13:25:49 -0400 From: "Jean-Martin Lapointe" Subject: [Histonet] IHC pos. & neg. control question To: Message-ID: < BEFD613BD39142499989F836556DDC830127283C@ACE.accellab.lan> Content-Type: text/plain; charset="iso-8859-1" Hi Curt, I agree with your pathologist. The section that you use as a negative control (without primary) in an IHC run should ideally be tissue that is a known positive, and of the same nature as the test sample. Therefore the best sample is often a section of your positive control tissue. The purpose of this negative control is to make sure that any positive staining observed in the test sample is not due to a spurious cross-reaction, unrelated to the primary. I don't think the issue per se is that you are using tissue from the same patient; rather, it is that you are using a sample for which the staining characteristics are not known. For instance, if are using a lymph node section as a negative, for a stain that targets an epithelial marker (eg Her2) in the test breast sample, then your negative tissue is not appropriate, because since lymph node contains no epithelial tissue, it will not stain no matter what. Therefore if your test sample shows a positive reaction in the epithelial tissue, but for some reason that reaction is a spurious false-positive, then the lymph node negative will not reveal that. I realize that this is all very theoretical and hypothetical, but I understand the pathologist wanting to be confident in the knowledge that all potential technical issues are eliminated before making his diagnosis. Jean-Martin From liz <@t> premierlab.com Fri May 20 15:25:12 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri May 20 15:25:15 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: Message-ID: Amos Isotype negative controls are based upon protein concentration not dilution. They must be the same protein concentration of the primary antibody at the dilution you are using. Using the same dilution will not work unless both stock solutions (primary antibody and isotype control) are at the same protein concentration which is rarely the case. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, May 20, 2011 2:16 PM To: jm.lapointe@accellab.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question Hi, This is simply a question of definitions. They are actually both right. If you have the patient tissue as a negative control you are not using the primary antibody on it but are replacing it (ideally) with an Ig with the same isotype AND dilution (universal negative is stupid). So if your primary is an IgG1 from a mouse and you use it at 1:100, your negative control would be a non-immune mouse serum IgG1 diluted to 1:100. Running this on unrelated material will not show you anything in this case. what it will show you is that the detection system you are using is specific to the antibody you put on the tissue and not to A) something else in the tissue or B) something on the Ig that is not the epitope itself. Although, If you run the same primary antibody on tissue that you expect will not react with the primary antibody, this would prove that the antibody reaction is specific to the antigenin question. This does raise the question of what to do about ubiquitous antigens (like ubiquitin) that are actually everywhere. Every cell has a cell cycle so they will all at one point or another show proliferation or degeneration for example. Does this mean you don't run a negative control? No you need to look at it in the perspective of expected results. There are various ways of using negative (and positive) controls. It would actually be cost prohibitive to run ALL the possible positive and negative controls for every test that we as histologists do. We need to discuss with our pathologists what they want to have done to give them the confidence in our testing to support their diagnosis. But remember that just because another lab and another pathologist does it differently doesn't mean it is wrong. It's just different. Happy Friday Folks, Amos Message: 5 Date: Thu, 19 May 2011 13:25:49 -0400 From: "Jean-Martin Lapointe" Subject: [Histonet] IHC pos. & neg. control question To: Message-ID: < BEFD613BD39142499989F836556DDC830127283C@ACE.accellab.lan> Content-Type: text/plain; charset="iso-8859-1" Hi Curt, I agree with your pathologist. The section that you use as a negative control (without primary) in an IHC run should ideally be tissue that is a known positive, and of the same nature as the test sample. Therefore the best sample is often a section of your positive control tissue. The purpose of this negative control is to make sure that any positive staining observed in the test sample is not due to a spurious cross-reaction, unrelated to the primary. I don't think the issue per se is that you are using tissue from the same patient; rather, it is that you are using a sample for which the staining characteristics are not known. For instance, if are using a lymph node section as a negative, for a stain that targets an epithelial marker (eg Her2) in the test breast sample, then your negative tissue is not appropriate, because since lymph node contains no epithelial tissue, it will not stain no matter what. Therefore if your test sample shows a positive reaction in the epithelial tissue, but for some reason that reaction is a spurious false-positive, then the lymph node negative will not reveal that. I realize that this is all very theoretical and hypothetical, but I understand the pathologist wanting to be confident in the knowledge that all potential technical issues are eliminated before making his diagnosis. Jean-Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Fri May 20 15:25:27 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri May 20 15:25:31 2011 Subject: [Histonet] FW: How to remove DAB to restain with DAB Message-ID: Hi, There is not any practical way to remove the DAB. I think I read about boiling it in a salt (of some sort) solution removing the DAB. Honestly I never bothered trying it as you need to ask yourself "What is this doing to my target antigen?". You should consider just restaining it with the DAB in place using either Alkaline Phosphatase and Fast Red or using a fluorescent detection if the DAB is interfering with the signal. Amos Message: 2 Date: Thu, 19 May 2011 11:55:14 -0500 From: "Margaryan, Naira" Subject: [Histonet] FW: How to remove DAB to restain with DAB To: "histonet-request@lists.utsouthwestern.edu" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: < C1BA93040C6B9A4A8D84878F93FEC36A09B60C525E@CMHEXCC01MBX.childrensmemorial.org > Content-Type: text/plain; charset="us-ascii" Hello, I would like to repeat my DAB staining on the same slide with DAB I run before. I know that "acid alcohol" will remove hematoxylin but how to remove DAB? I appreciate to any suggestion. Thanks in advance, Naira From amosbrooks <@t> gmail.com Fri May 20 15:56:47 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri May 20 15:56:51 2011 Subject: [Histonet] Alizarin Red Troubleshooting Message-ID: Hi, I seldom do this, but I'll bet I know EXACTLY what the problem is. Try it again and stop the rehydration at 95% ETOH then put it directly into the Alizarin Red (double check the pH of course). This will surely fix it. The calcium gets rinsed out when it is rehydrated. The same thing happens with Von Kossa. I also try to abbreviate rinsing after when dehydrating to coverslipping. Let us know if you still have trouble, Amos Message: 18 Date: Thu, 19 May 2011 09:30:27 -0400 From: "Phipps, Amanda" Subject: [Histonet] Alizarin Red Troubleshooting To: "'histonet@lists.utsouthwestern.edu'" Message-ID: < 20CE7246011C12489BA04BEB85CCDA1F0351BF252B@res2k7ms01> Content-Type: text/plain; charset="us-ascii" Hi All We are currently trying to stain for calcium deposits using Alizarin Red. A few months ago our protocol worked fine, but this time we are getting zero staining. Reagent has been made fresh, and pH is between 4.1 and 4.2. Here is our protocol. Deparaffinize to distilled H20 Stain slides with Alizarin Red S for 30 seconds to 5 minutes and observe reaction microscopically Shake off excess dye and blot sections Dehydrate in acetone 10 seconds to 20 seconds, then acetone-xylene (1:1) solution 10-20 seconds Clear in xylene and mount in synthetic mounting medium. Any help is greatly appreciated!! Amanda Phipps, HTL (ASCP) Histotechnologist Morphology Core Lab The Research Institute at Nationwide Children's Hospital 700 Children's Drive Columbus, Ohio 43205 (614) 355-3474 From amosbrooks <@t> gmail.com Fri May 20 16:04:50 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri May 20 16:04:55 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: References: Message-ID: Thanks Liz, You are absolutly right there, but have you ever noticed some folks eyes glaze over when you say that they must both be run at the same ug/mL? I would be lying if I said it never happened to me until I forced myself to work it out. Once you do though it isn't too bad. Unfortunately people don't think about this much. They want to put a slide on a machine and walk away without thinking about what they are doing. I guess I was oversimplifying the description. Thanks for pointing that out as it is actually an important aspect to the dilutions. Amos On Fri, May 20, 2011 at 4:25 PM, Liz Chlipala wrote: > Amos > > Isotype negative controls are based upon protein concentration not > dilution. They must be the same protein concentration of the primary > antibody at the dilution you are using. Using the same dilution will > not work unless both stock solutions (primary antibody and isotype > control) are at the same protein concentration which is rarely the case. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, Colorado 80308 > office (303) 682-3949 > fax (303) 682-9060 > www.premierlab.com > > > Ship to Address: > 1567 Skyway Drive, Unit E > Longmont, Colorado 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos > Brooks > Sent: Friday, May 20, 2011 2:16 PM > To: jm.lapointe@accellab.com; histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC pos. & neg. control question > > Hi, > This is simply a question of definitions. They are actually both > right. > If you have the patient tissue as a negative control you are not using > the > primary antibody on it but are replacing it (ideally) with an Ig with > the > same isotype AND dilution (universal negative is stupid). So if your > primary > is an IgG1 from a mouse and you use it at 1:100, your negative control > would > be a non-immune mouse serum IgG1 diluted to 1:100. Running this on > unrelated > material will not show you anything in this case. what it will show you > is > that the detection system you are using is specific to the antibody you > put > on the tissue and not to A) something else in the tissue or B) something > on > the Ig that is not the epitope itself. > Although, If you run the same primary antibody on tissue that you > expect will not react with the primary antibody, this would prove that > the > antibody reaction is specific to the antigenin question. This does raise > the > question of what to do about ubiquitous antigens (like ubiquitin) that > are > actually everywhere. Every cell has a cell cycle so they will all at one > point or another show proliferation or degeneration for example. Does > this > mean you don't run a negative control? No you need to look at it in the > perspective of expected results. > There are various ways of using negative (and positive) controls. > It > would actually be cost prohibitive to run ALL the possible positive and > negative controls for every test that we as histologists do. We need to > discuss with our pathologists what they want to have done to give them > the > confidence in our testing to support their diagnosis. But remember that > just > because another lab and another pathologist does it differently doesn't > mean > it is wrong. It's just different. > > Happy Friday Folks, > Amos > > > Message: 5 > Date: Thu, 19 May 2011 13:25:49 -0400 > From: "Jean-Martin Lapointe" > Subject: [Histonet] IHC pos. & neg. control question > To: > Message-ID: > < > BEFD613BD39142499989F836556DDC830127283C@ACE.accellab.lan> > Content-Type: text/plain; charset="iso-8859-1" > > Hi Curt, > I agree with your pathologist. The section that you use as a negative > control (without primary) in an IHC run should ideally be tissue that is > a > known positive, and of the same nature as the test sample. Therefore the > best sample is often a section of your positive control tissue. > The purpose of this negative control is to make sure that any positive > staining observed in the test sample is not due to a spurious > cross-reaction, unrelated to the primary. I don't think the issue per se > is > that you are using tissue from the same patient; rather, it is that you > are > using a sample for which the staining characteristics are not known. For > instance, if are using a lymph node section as a negative, for a stain > that > targets an epithelial marker (eg Her2) in the test breast sample, then > your > negative tissue is not appropriate, because since lymph node contains no > epithelial tissue, it will not stain no matter what. Therefore if your > test > sample shows a positive reaction in the epithelial tissue, but for some > reason that reaction is a spurious false-positive, then the lymph node > negative will not reveal that. > I realize that this is all very theoretical and hypothetical, but I > understand the pathologist wanting to be confident in the knowledge that > all > potential technical issues are eliminated before making his diagnosis. > > Jean-Martin > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From liz <@t> premierlab.com Fri May 20 16:24:15 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri May 20 16:24:23 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: Message-ID: No problem Amos, I have a one page handout that I use for workshops and presentations on how to do the math on this, it can be tricky, so if anyone is interested just e-mail me. Since we are talking about matched isotype negative controls, essentially three things need to be taken into consideration: 1. The isotype of the primary antibody 2. The protein concentration of the working dilution of the primary antibody 3. The antibody form - (affinity purified, culture supernatant, ascities fluid, etc.) the isotype negative control needs to match the primary antibody - some spec sheets list appropriate isotype negative control reagents some do not There is one caveat that I would like to address for those that are working with animal tissues - you need to check the spec sheet on your negative control reagents to make sure that it does not cross react with the species you are working with. If it does then you may get staining in your negative control slide. Here is a table that was generated from one of the older versions of the dako handbook that addresses the antibody form and suggested negative control reagents. Primary Antibody Type Suggested Negative Control Reagent Monoclonal Mouse - produced in ascites Antibody produced in ascites, same isotype as the primary antibody OR Normal nonimmune mouse serum Monoclonal Mouse - produced in tissue culture Antibody produced in tissue culture, same isotype as the primary antibody Polyclonal Rabbit or Goat - immunoglobulin fraction Normal rabbit or goat immunoglobulin fraction Solid-phase absorbed Polyclonal Rabbit - immunoglobulin fraction Rabbit immunoglobulin fraction - solid phase absorbed Polyclonal Rabbit - whole serum Normal or nonimmune rabbit serum, whole serum Hope this helps, and everyone have a great weekend Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Amos Brooks [mailto:amosbrooks@gmail.com] Sent: Friday, May 20, 2011 3:05 PM To: Liz Chlipala Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC pos. & neg. control question Thanks Liz, You are absolutly right there, but have you ever noticed some folks eyes glaze over when you say that they must both be run at the same ug/mL? I would be lying if I said it never happened to me until I forced myself to work it out. Once you do though it isn't too bad. Unfortunately people don't think about this much. They want to put a slide on a machine and walk away without thinking about what they are doing. I guess I was oversimplifying the description. Thanks for pointing that out as it is actually an important aspect to the dilutions. Amos On Fri, May 20, 2011 at 4:25 PM, Liz Chlipala wrote: Amos Isotype negative controls are based upon protein concentration not dilution. They must be the same protein concentration of the primary antibody at the dilution you are using. Using the same dilution will not work unless both stock solutions (primary antibody and isotype control) are at the same protein concentration which is rarely the case. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, May 20, 2011 2:16 PM To: jm.lapointe@accellab.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. & neg. control question Hi, This is simply a question of definitions. They are actually both right. If you have the patient tissue as a negative control you are not using the primary antibody on it but are replacing it (ideally) with an Ig with the same isotype AND dilution (universal negative is stupid). So if your primary is an IgG1 from a mouse and you use it at 1:100, your negative control would be a non-immune mouse serum IgG1 diluted to 1:100. Running this on unrelated material will not show you anything in this case. what it will show you is that the detection system you are using is specific to the antibody you put on the tissue and not to A) something else in the tissue or B) something on the Ig that is not the epitope itself. Although, If you run the same primary antibody on tissue that you expect will not react with the primary antibody, this would prove that the antibody reaction is specific to the antigenin question. This does raise the question of what to do about ubiquitous antigens (like ubiquitin) that are actually everywhere. Every cell has a cell cycle so they will all at one point or another show proliferation or degeneration for example. Does this mean you don't run a negative control? No you need to look at it in the perspective of expected results. There are various ways of using negative (and positive) controls. It would actually be cost prohibitive to run ALL the possible positive and negative controls for every test that we as histologists do. We need to discuss with our pathologists what they want to have done to give them the confidence in our testing to support their diagnosis. But remember that just because another lab and another pathologist does it differently doesn't mean it is wrong. It's just different. Happy Friday Folks, Amos Message: 5 Date: Thu, 19 May 2011 13:25:49 -0400 From: "Jean-Martin Lapointe" Subject: [Histonet] IHC pos. & neg. control question To: Message-ID: < BEFD613BD39142499989F836556DDC830127283C@ACE.accellab.lan> Content-Type: text/plain; charset="iso-8859-1" Hi Curt, I agree with your pathologist. The section that you use as a negative control (without primary) in an IHC run should ideally be tissue that is a known positive, and of the same nature as the test sample. Therefore the best sample is often a section of your positive control tissue. The purpose of this negative control is to make sure that any positive staining observed in the test sample is not due to a spurious cross-reaction, unrelated to the primary. I don't think the issue per se is that you are using tissue from the same patient; rather, it is that you are using a sample for which the staining characteristics are not known. For instance, if are using a lymph node section as a negative, for a stain that targets an epithelial marker (eg Her2) in the test breast sample, then your negative tissue is not appropriate, because since lymph node contains no epithelial tissue, it will not stain no matter what. Therefore if your test sample shows a positive reaction in the epithelial tissue, but for some reason that reaction is a spurious false-positive, then the lymph node negative will not reveal that. I realize that this is all very theoretical and hypothetical, but I understand the pathologist wanting to be confident in the knowledge that all potential technical issues are eliminated before making his diagnosis. Jean-Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat May 21 10:31:51 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat May 21 10:31:56 2011 Subject: [Histonet] FW: How to remove DAB to restain with DAB In-Reply-To: References: Message-ID: I have removed DAB by using the probe cleaning reagents from the Dako Autostainer but Amos is right this destroys the antigen site so it is of no use. If you really only have that one section I guess you might be able to use it as an H&E. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, May 20, 2011 2:25 PM To: NMargaryan@childrensmemorial.org; histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: How to remove DAB to restain with DAB Hi, There is not any practical way to remove the DAB. I think I read about boiling it in a salt (of some sort) solution removing the DAB. Honestly I never bothered trying it as you need to ask yourself "What is this doing to my target antigen?". You should consider just restaining it with the DAB in place using either Alkaline Phosphatase and Fast Red or using a fluorescent detection if the DAB is interfering with the signal. Amos Message: 2 Date: Thu, 19 May 2011 11:55:14 -0500 From: "Margaryan, Naira" Subject: [Histonet] FW: How to remove DAB to restain with DAB To: "histonet-request@lists.utsouthwestern.edu" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: < C1BA93040C6B9A4A8D84878F93FEC36A09B60C525E@CMHEXCC01MBX.childrensmemorial.or g > Content-Type: text/plain; charset="us-ascii" Hello, I would like to repeat my DAB staining on the same slide with DAB I run before. I know that "acid alcohol" will remove hematoxylin but how to remove DAB? I appreciate to any suggestion. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat May 21 10:44:52 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat May 21 10:44:56 2011 Subject: [Histonet] Formalin fixed frozen sections In-Reply-To: References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5752@PHSXMB30.partners.org> Message-ID: <58765D37EF084EF99ACCC61D937187BC@prueggihctechlt> I fix frozen tissue in reg 10% NB formalin and then process it into paraffin all the time. I haven't tried it but if someone gave me some unfixed frozen tissue (especially bone) and wanted me to do frozen tape sectioning I would recommend that we thaw fix the original frozen block in formalin, then infiltrate in sucrose and re snap freeze it as they would section better. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of amitapandey@torrentpharma.com Sent: Wednesday, May 18, 2011 10:02 PM To: Sherwood, Margaret Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin fixed frozen sections We take frozen section from formalin fixed tissue and placed them in 30% sucrose overnight before mounting on chucks. But never tried the other way round frozen tissue then fixed formalin, i heard that it helps in falling of the tissue. But no experience. Amita From: "Sherwood, Margaret " To: Date: 19/05/11 01:21 AM Subject: Re: [Histonet] Formalin fixed frozen sections Sent by: histonet-bounces@lists.utsouthwestern.edu Does anyone fix their frozen tissue first in formalin before embedding and sectioning? I know one investigator in our lab did that previously, but was not sure of the strength of formalin (I don't believe it was 10%). Another investigator inquired about doing that. I would appreciate hearing from fellow histonetters. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat May 21 10:49:13 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat May 21 10:49:17 2011 Subject: [Histonet] Alizarin Red Troubleshooting In-Reply-To: References: Message-ID: <26AEC104030443C8A197F0B36E472FF0@prueggihctechlt> Bingo, that is exactly what I was thinking. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, May 20, 2011 2:57 PM To: Amanda.Phipps@nationwidechildrens.org; histonet@lists.utsouthwestern.edu Subject: [Histonet] Alizarin Red Troubleshooting Hi, I seldom do this, but I'll bet I know EXACTLY what the problem is. Try it again and stop the rehydration at 95% ETOH then put it directly into the Alizarin Red (double check the pH of course). This will surely fix it. The calcium gets rinsed out when it is rehydrated. The same thing happens with Von Kossa. I also try to abbreviate rinsing after when dehydrating to coverslipping. Let us know if you still have trouble, Amos Message: 18 Date: Thu, 19 May 2011 09:30:27 -0400 From: "Phipps, Amanda" Subject: [Histonet] Alizarin Red Troubleshooting To: "'histonet@lists.utsouthwestern.edu'" Message-ID: < 20CE7246011C12489BA04BEB85CCDA1F0351BF252B@res2k7ms01> Content-Type: text/plain; charset="us-ascii" Hi All We are currently trying to stain for calcium deposits using Alizarin Red. A few months ago our protocol worked fine, but this time we are getting zero staining. Reagent has been made fresh, and pH is between 4.1 and 4.2. Here is our protocol. Deparaffinize to distilled H20 Stain slides with Alizarin Red S for 30 seconds to 5 minutes and observe reaction microscopically Shake off excess dye and blot sections Dehydrate in acetone 10 seconds to 20 seconds, then acetone-xylene (1:1) solution 10-20 seconds Clear in xylene and mount in synthetic mounting medium. Any help is greatly appreciated!! Amanda Phipps, HTL (ASCP) Histotechnologist Morphology Core Lab The Research Institute at Nationwide Children's Hospital 700 Children's Drive Columbus, Ohio 43205 (614) 355-3474 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Sun May 22 04:43:07 2011 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sun May 22 04:43:05 2011 Subject: [Histonet] to Rene J Buesa Message-ID: <1585823684.20110522134307@mail.ru> I am sorry, that uses histonet for personal goals. Dear Rene! Last 3 my answers for you are returned back to me. Your e-mails I got successfully. Support service of mail.ru wrote that problem is at yahoo.com server. Please check your postmaster service. Here is my post-address: Maxim Peshkov, ul. Mikhailovskaya, 90-A, 347909, Taganrog, Russia. Sincerely, Maxim. mailto:Maxim_71@mail.ru From cdisbrow <@t> msn.com Sun May 22 21:00:05 2011 From: cdisbrow <@t> msn.com (Carrie Disbrow) Date: Sun May 22 21:00:09 2011 Subject: [Histonet] Immuno controls In-Reply-To: References: Message-ID: Hi all, After consideration I disagree with the consensus and also agree with Curt's pathologist. More supplies and labor will be involved as cutting more controls will be neccessay to meet the requests of the pathologist. The patient tissue is put on two controls slides. One slide is used as the negative control and has both the patient tissue and the known positive control which should both be negative after the run. The other slide with the known positive control and the patient tissue in question is also stained with the positive control staining positive. Thank you! From mab70 <@t> medschl.cam.ac.uk Mon May 23 03:04:54 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Mon May 23 03:05:08 2011 Subject: [Histonet] This is goodbye Message-ID: To everyone in histoland, This is my last full week at work. From next Tuesday I will be retiring from my job at Cambridge University. I have thoroughly enjoyed my chosen profession as a research technician specialising in histology. I realise that because I have always worked in research my experience is somewhat limited: I haven't been asked for the same range of techniques that you find in a routine clinical lab. However, I have enjoyed my work and have learnt and devised some techniques for a range of interesting samples. Now it's time to learn something new! I hope to spend more time with my lace pillow and learn to do it properly, maybe learn to do Northamptonshire lace which I believe is a variation on Bucks point. (I come originally from Northants) I have had a go at Bedfordshire and will learn more, it is very pretty. I also plan to travel; my next trip is to New Zealand in November. I won't go on. I just want to say this: You are all great guys and girls; you share your experience and friendship with great generosity, which I have always felt is what a scientist should do. Thank you for your comradeship and help over the years. Maybe I'll get to meet some of you by chance during my travels. Best wishes to you all and a great big Thank You, From Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 From Carmen.M.Garcia <@t> uv.es Mon May 23 03:35:20 2011 From: Carmen.M.Garcia <@t> uv.es (Carmen Maria Garcia Pascual) Date: Mon May 23 03:34:04 2011 Subject: [Histonet] In vitro mouse embryo culture In-Reply-To: References: Message-ID: <6395165382carmaga6@uv.es> Good Moorning to everyone: I am Carmen Garcia a PhD student from Valencia, we are know interesting in make in vitro culture of mouse embryos to do toxicity tests and in our lab we have not experience. someone can give me some advise? or recommend me some bibliography? how long can I have the embryos in culture? thank you so much!!! Best regards, Carmen From rjbuesa <@t> yahoo.com Mon May 23 09:48:50 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 23 09:48:54 2011 Subject: [Histonet] Immuno controls In-Reply-To: References: Message-ID: <87688.31423.qm@web65714.mail.ac4.yahoo.com> Also Sprach Zarathustra!!!! Ren? J. From: Carrie Disbrow To: histonet@lists.utsouthwestern.edu Sent: Sunday, May 22, 2011 10:00 PM Subject: [Histonet] Immuno controls Hi all, After consideration I disagree with the consensus and also agree with Curt's pathologist. More supplies and labor will be? involved as? cutting more controls will be neccessay to meet the requests of the pathologist. The patient tissue is put on two controls slides. One slide is used as the negative control and has both the patient tissue and the known positive control which should both be negative after the run. The other slide with the known positive control and the patient tissue in question is also stained with the positive control staining positive. Thank you! ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbarone <@t> NEMOURS.ORG Mon May 23 10:02:16 2011 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon May 23 10:02:19 2011 Subject: [Histonet] RE: Histonet Digest, Vol 90, Issue 27 In-Reply-To: References: Message-ID: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, May 22, 2011 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 90, Issue 27 Send Histonet mailing list submissions to To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. to Rene J Buesa (Maxim Peshkov) ---------------------------------------------------------------------- Message: 1 Date: Sun, 22 May 2011 13:43:07 +0400 From: Maxim Peshkov Subject: [Histonet] to Rene J Buesa To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu Message-ID: <1585823684.20110522134307@mail.ru> Content-Type: text/plain; charset=windows-1251 I am sorry, that uses histonet for personal goals. Dear Rene! Last 3 my answers for you are returned back to me. Your e-mails I got successfully. Support service of mail.ru wrote that problem is at yahoo.com server. Please check your postmaster service. Here is my post-address: Maxim Peshkov, ul. Mikhailovskaya, 90-A, 347909, Taganrog, Russia. Sincerely, Maxim. mailto:Maxim_71@mail.ru ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 90, Issue 27 **************************************** From cbarone <@t> NEMOURS.ORG Mon May 23 10:15:34 2011 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon May 23 10:15:37 2011 Subject: [Histonet] RE: Histonet Digest, Vol 90, Issue 27 In-Reply-To: References: Message-ID: Histonetters: Needed to stain xenograft with "Bunny"-IgG-direct, that had already been in formailin two weeks (was FFPE). Normally would do on a frozen 1:40? ....We have background everywhwere on first run...suggestions? Neg is also shining??? Crazy researcher's! Got to love them...when will they learn to ask first? We are diluting primary in plan two, and will stain over-night at 4 degrees....but, this thing is lit-up like the SUN! I was expecting low signal? Go figure? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, May 22, 2011 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 90, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. to Rene J Buesa (Maxim Peshkov) ---------------------------------------------------------------------- Message: 1 Date: Sun, 22 May 2011 13:43:07 +0400 From: Maxim Peshkov Subject: [Histonet] to Rene J Buesa To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu Message-ID: <1585823684.20110522134307@mail.ru> Content-Type: text/plain; charset=windows-1251 I am sorry, that uses histonet for personal goals. Dear Rene! Last 3 my answers for you are returned back to me. Your e-mails I got successfully. Support service of mail.ru wrote that problem is at yahoo.com server. Please check your postmaster service. Here is my post-address: Maxim Peshkov, ul. Mikhailovskaya, 90-A, 347909, Taganrog, Russia. Sincerely, Maxim. mailto:Maxim_71@mail.ru ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 90, Issue 27 **************************************** From Allison_Scott <@t> hchd.tmc.edu Mon May 23 11:22:24 2011 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Mon May 23 11:22:27 2011 Subject: [Histonet] Freezing bath solutions Message-ID: <1872B4A455B7974391609AD8034C79FC026DFDD9@LBEXCH01.hchd.local> Hello to all in histoland. What type of solutions are being used if any to freezze your chucks for frozen sections. Our sister hospital is using methyl butane and there was a flash fire last week. We use the same solution. Any suggestions will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From jclark <@t> pcnm.com Mon May 23 11:31:08 2011 From: jclark <@t> pcnm.com (Joanne Clark) Date: Mon May 23 11:40:17 2011 Subject: [Histonet] p63 Message-ID: <0494A7D4E8CC254EA2FB81464982E3786DCA72@S10MAILD001N1.SH10.lan> I need to find a source for p63 antibody. Where does everyone purchase theirs from? Joanne Clark,HT Pathology Consultants of New Mexico From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon May 23 12:33:27 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon May 23 12:33:44 2011 Subject: [Histonet] RE: p63 In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3786DCA72@S10MAILD001N1.SH10.lan> References: <0494A7D4E8CC254EA2FB81464982E3786DCA72@S10MAILD001N1.SH10.lan> Message-ID: Biocare Medical Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark [jclark@pcnm.com] Sent: Monday, May 23, 2011 12:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p63 I need to find a source for p63 antibody. Where does everyone purchase theirs from? Joanne Clark,HT Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From boneslides <@t> aol.com Mon May 23 12:41:36 2011 From: boneslides <@t> aol.com (boneslides@aol.com) Date: Mon May 23 12:41:51 2011 Subject: [Histonet] Re: Histonet Digest, Vol 90, Issue 28 Message-ID: <8CDE787CC364FAB-17A0-7F431@webmail-d058.sysops.aol.com> Dear Miss Margaret Blount: Congratulations on your retirement!! May you enjoy good health and many years doing the things you WANT to do!! God Bless! -----Original Message----- From: histonet-request To: histonet Sent: Mon, May 23, 2011 1:00 pm Subject: Histonet Digest, Vol 90, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet r, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific han "Re: Contents of Histonet digest..." oday's Topics: 1. Immuno controls (Carrie Disbrow) 2. This is goodbye (Margaret Blount) 3. In vitro mouse embryo culture (Carmen Maria Garcia Pascual) 4. Re: Immuno controls (Rene J Buesa) 5. RE: Histonet Digest, Vol 90, Issue 27 (Barone, Carol ) 6. RE: Histonet Digest, Vol 90, Issue 27 (Barone, Carol ) 7. Freezing bath solutions (Scott, Allison D) 8. p63 (Joanne Clark) --------------------------------------------------------------------- Message: 1 ate: Sun, 22 May 2011 22:00:05 -0400 rom: Carrie Disbrow ubject: [Histonet] Immuno controls o: essage-ID: ontent-Type: text/plain; charset="iso-8859-1" i all, fter consideration I disagree with the consensus and also agree with Curt's athologist. More supplies and labor will be involved as cutting more controls ill be neccessay to meet the requests of the pathologist. The patient tissue is ut on two controls slides. One slide is used as the negative control and has oth the patient tissue and the known positive control which should both be egative after the run. The other slide with the known positive control and the atient tissue in question is also stained with the positive control staining ositive. hank you! ------------------------------ Message: 2 ate: Mon, 23 May 2011 09:04:54 +0100 rom: "Margaret Blount" ubject: [Histonet] This is goodbye o: essage-ID: ontent-Type: text/plain; charset="US-ASCII" To everyone in histoland, This is my last full week at work. From next Tuesday I will be retiring rom my job at Cambridge University. I have thoroughly enjoyed my chosen rofession as a research technician specialising in histology. I realise hat because I have always worked in research my experience is somewhat imited: I haven't been asked for the same range of techniques that you ind in a routine clinical lab. However, I have enjoyed my work and have earnt and devised some techniques for a range of interesting samples. ow it's time to learn something new! I hope to spend more time with my lace pillow and learn to do it roperly, maybe learn to do Northamptonshire lace which I believe is a ariation on Bucks point. (I come originally from Northants) I have had go at Bedfordshire and will learn more, it is very pretty. I also plan o travel; my next trip is to New Zealand in November. I won't go on. I just want to say this: You are all great guys and irls; you share your experience and friendship with great generosity, hich I have always felt is what a scientist should do. Thank you for our comradeship and help over the years. Maybe I'll get to meet some of ou by chance during my travels. Best wishes to you all and a great big Thank You, From Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 ------------------------------ Message: 3 ate: Mon, 23 May 2011 10:35:20 +0200 (CEST) rom: "Carmen Maria Garcia Pascual" ubject: [Histonet] In vitro mouse embryo culture o: histonet@lists.utsouthwestern.edu essage-ID: <6395165382carmaga6@uv.es> ontent-Type: text/plain; charset="ISO-8859-1" Good Moorning to everyone: I am Carmen Garcia a PhD student from Valencia, we are know interesting n make in vitro culture of mouse embryos to do toxicity tests and in ur lab we have not experience. someone can give me some advise? or ecommend me some bibliography? ow long can I have the embryos in culture? hank you so much!!! Best regards, armen ------------------------------ Message: 4 ate: Mon, 23 May 2011 07:48:50 -0700 (PDT) rom: Rene J Buesa ubject: Re: [Histonet] Immuno controls o: Carrie Disbrow , "histonet@lists.utsouthwestern.edu" essage-ID: <87688.31423.qm@web65714.mail.ac4.yahoo.com> ontent-Type: text/plain; charset=iso-8859-1 Also Sprach Zarathustra!!!! en? J. From: Carrie Disbrow o: histonet@lists.utsouthwestern.edu ent: Sunday, May 22, 2011 10:00 PM ubject: [Histonet] Immuno controls i all, fter consideration I disagree with the consensus and also agree with Curt's athologist. More supplies and labor will be? involved as? cutting more controls ill be neccessay to meet the requests of the pathologist. The patient tissue is ut on two controls slides. One slide is used as the negative control and has oth the patient tissue and the known positive control which should both be egative after the run. The other slide with the known positive control and the atient tissue in question is also stained with the positive control staining ositive. hank you! ??? ??? ??? ? ??? ??? ? _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 ate: Mon, 23 May 2011 11:02:16 -0400 rom: "Barone, Carol " ubject: [Histonet] RE: Histonet Digest, Vol 90, Issue 27 o: essage-ID: ontent-Type: text/plain; charset="us-ascii" -----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of istonet-request@lists.utsouthwestern.edu ent: Sunday, May 22, 2011 1:01 PM o: histonet@lists.utsouthwestern.edu ubject: Histonet Digest, Vol 90, Issue 27 Send Histonet mailing list submissions to To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet r, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest..." oday's Topics: 1. to Rene J Buesa (Maxim Peshkov) --------------------------------------------------------------------- Message: 1 ate: Sun, 22 May 2011 13:43:07 +0400 rom: Maxim Peshkov ubject: [Histonet] to Rene J Buesa o: Rene J Buesa c: histonet@lists.utsouthwestern.edu essage-ID: <1585823684.20110522134307@mail.ru> ontent-Type: text/plain; charset=windows-1251 I am sorry, that uses histonet for personal goals. Dear Rene! ast 3 my answers for you are returned back to me. our e-mails I got successfully. upport service of mail.ru wrote that problem is at yahoo.com server. lease check your postmaster service. Here is my post-address: axim Peshkov, l. Mikhailovskaya, 90-A, 47909, aganrog, ussia. Sincerely, axim. mailto:Maxim_71@mail.ru ----------------------------- _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 90, Issue 27 *************************************** ------------------------------ Message: 6 ate: Mon, 23 May 2011 11:15:34 -0400 rom: "Barone, Carol " ubject: [Histonet] RE: Histonet Digest, Vol 90, Issue 27 o: essage-ID: ontent-Type: text/plain; charset="us-ascii" Histonetters: Needed to stain xenograft with "Bunny"-IgG-direct, that ad already been in formailin two weeks (was FFPE). Normally would do n a frozen 1:40? ....We have background everywhwere on first un...suggestions? Neg is also shining??? Crazy researcher's! Got to ove them...when will they learn to ask first? We are diluting primary in plan two, and will stain over-night at 4 egrees....but, this thing is lit-up like the SUN! was expecting low signal? Go figure? ----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of istonet-request@lists.utsouthwestern.edu ent: Sunday, May 22, 2011 1:01 PM o: histonet@lists.utsouthwestern.edu ubject: Histonet Digest, Vol 90, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet r, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest..." oday's Topics: 1. to Rene J Buesa (Maxim Peshkov) --------------------------------------------------------------------- Message: 1 ate: Sun, 22 May 2011 13:43:07 +0400 rom: Maxim Peshkov ubject: [Histonet] to Rene J Buesa o: Rene J Buesa c: histonet@lists.utsouthwestern.edu essage-ID: <1585823684.20110522134307@mail.ru> ontent-Type: text/plain; charset=windows-1251 I am sorry, that uses histonet for personal goals. Dear Rene! ast 3 my answers for you are returned back to me. our e-mails I got successfully. upport service of mail.ru wrote that problem is at yahoo.com server. lease check your postmaster service. Here is my post-address: axim Peshkov, l. Mikhailovskaya, 90-A, 47909, aganrog, ussia. Sincerely, axim. mailto:Maxim_71@mail.ru ----------------------------- _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 90, Issue 27 *************************************** ------------------------------ Message: 7 ate: Mon, 23 May 2011 11:22:24 -0500 rom: "Scott, Allison D" ubject: [Histonet] Freezing bath solutions o: essage-ID: <1872B4A455B7974391609AD8034C79FC026DFDD9@LBEXCH01.hchd.local> ontent-Type: text/plain; charset="us-ascii" Hello to all in histoland. What type of solutions are being used if any o freezze your chucks for frozen sections. Our sister hospital is sing methyl butane and there was a flash fire last week. We use the ame solution. Any suggestions will be greatly appreciated. Allison Scott HT(ASCP) istology Supervisor BJ Hospital ONFIDENTIALITY NOTICE: f you have received this e-mail in error, please immediately notify the ender by return e-mail and delete this e-mail and any attachments from our computer system. To the extent the information in this e-mail and any attachments contain rotected health information as defined by the Health Insurance Portability nd Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 64; or Chapter 181, Texas Health and Safety Code, it is confidential and/or rivileged. This e-mail may also be confidential and/or privileged under exas law. The e-mail is for the use of only the individual or entity named bove. If you are not the intended recipient, or any authorized epresentative of the intended recipient, you are hereby notified that any eview, dissemination or copying of this e-mail and its attachments is trictly prohibited. ------------------------------ Message: 8 ate: Mon, 23 May 2011 16:31:08 +0000 rom: Joanne Clark ubject: [Histonet] p63 o: "histonet@lists.utsouthwestern.edu" essage-ID: <0494A7D4E8CC254EA2FB81464982E3786DCA72@S10MAILD001N1.SH10.lan> ontent-Type: text/plain; charset="us-ascii" I need to find a source for p63 antibody. Where does everyone purchase theirs rom? Joanne Clark,HT athology Consultants of New Mexico ------------------------------ _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 90, Issue 28 * From Lynne.Bell <@t> cvmc.org Mon May 23 12:45:50 2011 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Mon May 23 12:45:55 2011 Subject: [Histonet] RE: p63 In-Reply-To: <0494A7D4E8CC254EA2FB81464982E3786DCA72@S10MAILD001N1.SH10.lan> References: <0494A7D4E8CC254EA2FB81464982E3786DCA72@S10MAILD001N1.SH10.lan> Message-ID: I believe the only place you can buy p63 is through Biocare, www.biocare.net. Lynne Bell, HT (ASCP) Histology Team Leader 802-371-4923 From rsrichmond <@t> gmail.com Mon May 23 13:12:37 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon May 23 13:12:41 2011 Subject: [Histonet] Re: Freezing bath solutions Message-ID: Allison Scott after a flash fire with the stuff asks about alternatives to 2-methylbutane for freezing specimens. There is indeed a safe alternative, though I haven't had a chance to try it. I've posted about it on Histonet more than once - here's a copy of an earlier post of mine: 3M(tm) Novec(tm) Engineered Fluid HFE-7100 This product belongs to a class of fluorocarbons called "segregated hydrofluoroethers (HFE's)" According to various MSDS, HFE-7100 is methyl nonafluoroisobutyl ether C4F9-O-CH3 It melts and freezes at -135 C, so that it would remain liquid in the Histobath. (It would freeze solid in liquid nitrogen, however.) It boils at 60 C., and is listed as non-flammable. It has almost no vapor pressure at freezing temperatures. It cost around $230 a gallon a few years ago. Bob Richmond Samurai Pathologist Knoxville TN From sally.norton <@t> seattlechildrens.org Mon May 23 13:28:28 2011 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Mon May 23 13:28:44 2011 Subject: [Histonet] Jones stain boo boo Message-ID: <16E0693C7018C245959AC729FE66EDE52DBECB@s107.childrens.sea.kids> I accidentally put my renal slides in the Methanimine Silver (after Periodic Acid) before putting them in the Thiosemicarbizine. Slides take on a yellow hue which makes the H&E counterstain muddy. Is there a way to correct this? Thank you, Sally Norton Histotech CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mwhite <@t> mcleodhealth.org Mon May 23 13:46:29 2011 From: mwhite <@t> mcleodhealth.org (mwhite@mcleodhealth.org) Date: Mon May 23 13:46:33 2011 Subject: [Histonet] Re: Freezing bath solutions In-Reply-To: References: Message-ID: Allison and Dr. Richmond: We are using Novec 7000 successfully in our freezing bath and have been doing so for about 6 months with no major problems. It does have some annoying traits ; however the safety benefits outweigh those issues , in my opinion. The issues are: a little more time required for specimen to freeze, tissues tending to float in the solution, and evaporation. I worried that the evaporation would be a big problem , but it has not .My techs discovered through experimentation that by lowering the tissue slowly into the fluid, it would stop the flotation problem. Overall, I would recommend it. 3M has been very helpful to us. (I'm not being paid in any way to endorse this!!) See below for 3M contact information. Additionally, through earlier Histonet inquiries, I was told about a freezing bath for those of you who still use the old Histobath that is no longer made. If anyone wants information about it, It's on www.ftssystems.com and the item is called a "Mult-Cool Bath". We don't have one , but it looks like it could be a suitable replacement. Here's our purchasing contact person at 3M: Christina Cerecedes 1-888-509-5224 Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center Florence, SC (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmaster@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. From TGoins <@t> mt.gov Mon May 23 13:56:05 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Mon May 23 13:56:11 2011 Subject: [Histonet] IHC Control Tissues Message-ID: If you haven't made up your mind yet on this issue, here are a couple of references that may - or may not - help: Veterinary Diagnostics: http://www.ncbi.nlm.nih.gov/sites/entrez?orig_db=PubMed&db=pubmed&cmd=Search&term=%22Journal%20of%20veterinary%20diagnostic%20investigation%20%3A%20official%20publication%20of%20the%20American%20Association%20of%20Veterinary%20Laboratory%20Diagnosticians%2C%20Inc%22%5BJour%5D%20AND%2020%5Bvolume%5D%20AND%20393%5Bpage%5D%20AND%202008%5Bpdat%5D FDA F.20 Quality Control: http://www.fda.gov/downloads/MedicalDevices/DeviceRegulationandGuidance/GuidanceDocuments/ucm094015.pdf Tresa Goins Histopathology Supervisor Department of Livestock Bozeman, Montana From w_alkadhumi <@t> yahoo.com Mon May 23 14:23:54 2011 From: w_alkadhumi <@t> yahoo.com (wassan alkadhumi) Date: Mon May 23 14:23:56 2011 Subject: [Histonet] FISH Message-ID: <539226.53686.qm@web45202.mail.sp1.yahoo.com> Dear members ? We?just started doing FISH using HER2 FISH pharma Dx Dako kit, Code number K5331 on malignant breast tissues. ?I was wondering what is the success ratio or percentage for say a one run???my hospital is the first in our region?to start FISH ? and I am the first to run it. I have no experience? with the hybridizer but?by reading the?hybridizer manual and the kit insert i discover it is user friendly. I run the first batch (six slides only)?yesterday.?Continue and?finish it today. only three of the slides worked. I will start a new batch next week and I appreciate any help you may offer to avoid inaccurate result. ? Thank you ? Wassan Histotechnician Shorsh General Hospital North of Iraq From gu.lang <@t> gmx.at Mon May 23 15:15:37 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon May 23 15:15:44 2011 Subject: AW: [Histonet] FISH In-Reply-To: <539226.53686.qm@web45202.mail.sp1.yahoo.com> References: <539226.53686.qm@web45202.mail.sp1.yahoo.com> Message-ID: <00DDDF450F674127ACFF7D694679751A@dielangs.at> Hi Wassan, you may depart the FISH procedure in a tissue-dependant first part and a probe-dependant second part. The first part comprises the pretreatment of the tissue to retrieve and permeabilises it. The amount of pretreatment is dependant on the fixation-duration of the tissue. The longer the fixation - the higher the temperature of the buffer, the longer the incubation time in the buffer, the longer the incubation time in the protease. Troubleshooting in this part: Too low temperature of some slides because of inconsistent temperature in the waterbath. Tissueslides mounted on the glass-slides in different heights. Too short protease-incubation in relation to the fixation time. Usually nuclei can be digested until you see the first tiny holes. Underdigested nuclei are dense and look grey in the triplefilter. If the nuclei are overdigested, they get bigger holes and fuzzy borders. Different staining results due to variing fixation-times. The second part comprises the stringent wash. The temperature, buffer-quality, duration are probe-depending and are usually provided in the kit-handbook. Troubleshooting second part: Too high temp leads to loosing the bound probe. Too low temp leads to high background because of unspecifc bound probes. With your problem I would try to prolong the digestion in 10 min steps, until a good result is found. Hint: standardization of fixation is the crucial point. In our lab the tumor-block is immediately fixed in a cassette as a FISH-block. So it will be fixed very well and withstands the harsh treatment. The disadvantage is stronger autofluorescence. A further hint: be sure, that the gum around the coverslip is hardened before starting the hybridizer-protocol. 10 min drying time. I hope this helps Gudrun Lang -----Ursprüngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von wassan alkadhumi Gesendet: Montag, 23. Mai 2011 21:24 An: h n Betreff: [Histonet] FISH Dear members   We just started doing FISH using HER2 FISH pharma Dx Dako kit, Code number K5331 on malignant breast tissues.  I was wondering what is the success ratio or percentage for say a one run?? my hospital is the first in our region to start FISH   and I am the first to run it. I have no experience  with the hybridizer but by reading the hybridizer manual and the kit insert i discover it is user friendly. I run the first batch (six slides only) yesterday. Continue and finish it today. only three of the slides worked. I will start a new batch next week and I appreciate any help you may offer to avoid inaccurate result.   Thank you   Wassan Histotechnician Shorsh General Hospital North of Iraq _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> carisls.com Mon May 23 16:53:16 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Mon May 23 16:54:44 2011 Subject: [Histonet] Las Vegas Position Message-ID: <6F33D8418806044682A391273399860F08374FCA@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! We are a 5 physician gastroenterology practice located in Las Vegas, NV looking for a certified HT or HTL. Candidate must meet CLIA grossing requirements. The candidate will be responsible for routine histology duties. This is a part-time position that offers a competitive salary and flexible hours. Interested applicants should contact Meredith Hale, phone (214)596-2219 or e-mail mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 214-596-7095 mhale@carisls.com From mjdessoye <@t> wvhcs.org Tue May 24 06:14:23 2011 From: mjdessoye <@t> wvhcs.org (Dessoye, Michael J) Date: Tue May 24 06:15:42 2011 Subject: [Histonet] p63 References: <0494A7D4E8CC254EA2FB81464982E3786DCA72@S10MAILD001N1.SH10.lan> Message-ID: Biocare has a very good, and fairly inexpensive, p63 that we have had good results with: http://biocare.net/products/antibodies/p/163/ ________________________________ From: Joanne Clark [mailto:jclark@pcnm.com] Sent: Mon 5/23/2011 12:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p63 I need to find a source for p63 antibody. Where does everyone purchase theirs from? Joanne Clark,HT Pathology Consultants of New Mexico _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From mab70 <@t> medschl.cam.ac.uk Tue May 24 06:14:02 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Tue May 24 06:15:52 2011 Subject: [Histonet] Thank you Message-ID: Hello everyone! Thank you all for your good wishes on my retirement. I knew you were all great people: you have just proved it big time! Thank you all from the bottom of my heart. Keep smiling! Much love Margaret From abright <@t> brightinstruments.com Tue May 24 08:03:21 2011 From: abright <@t> brightinstruments.com (abright@brightinstruments.com) Date: Tue May 24 08:03:34 2011 Subject: [Histonet] Re: Freezing bath solutions In-Reply-To: References: Message-ID: <757762693-1306242203-cardhu_decombobulator_blackberry.rim.net-1362851250-@b15.c2.bise7.blackberry> Melanie, In our Clini-RF rapid freezer we have a device for keeping tissue submerged in Novec 7000. Regards Alan Bright www.brightinstruments.com Sent from my BlackBerry? wireless device -----Original Message----- From: mwhite@mcleodhealth.org Sender: histonet-bounces@lists.utsouthwestern.edu Date: Mon, 23 May 2011 14:46:29 To: Robert Richmond Cc: ; Subject: Re: [Histonet] Re: Freezing bath solutions Allison and Dr. Richmond: We are using Novec 7000 successfully in our freezing bath and have been doing so for about 6 months with no major problems. It does have some annoying traits ; however the safety benefits outweigh those issues , in my opinion. The issues are: a little more time required for specimen to freeze, tissues tending to float in the solution, and evaporation. I worried that the evaporation would be a big problem , but it has not .My techs discovered through experimentation that by lowering the tissue slowly into the fluid, it would stop the flotation problem. Overall, I would recommend it. 3M has been very helpful to us. (I'm not being paid in any way to endorse this!!) See below for 3M contact information. Additionally, through earlier Histonet inquiries, I was told about a freezing bath for those of you who still use the old Histobath that is no longer made. If anyone wants information about it, It's on www.ftssystems.com and the item is called a "Mult-Cool Bath". We don't have one , but it looks like it could be a suitable replacement. Here's our purchasing contact person at 3M: Christina Cerecedes 1-888-509-5224 Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center Florence, SC (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmaster@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING-LINKS ------------------------------------------------------ Teach SpamSniper if this mail (ID 01EL6KSKd) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01EL6KSKd&m=ce12d22f644f&t=20110523&c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01EL6KSKd&m=ce12d22f644f&t=20110523&c=n Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01EL6KSKd&m=ce12d22f644f&t=20110523&c=f ------------------------------------------------------ END-ANTISPAM-VOTING-LINKS From cpyse <@t> x-celllab.com Tue May 24 09:56:52 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue May 24 09:57:20 2011 Subject: [Histonet] decal Message-ID: <001101cc1a22$d100e9d0$7302bd70$@com> Hello Histonetters What is everyone using to decal specimens. I haven't had to use it in a few years, before that I just purchased formic acid and made a 10% solution. Just wanted to see if the criteria has changes over the years. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com From rjbuesa <@t> yahoo.com Tue May 24 10:50:13 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 24 10:50:16 2011 Subject: [Histonet] decal In-Reply-To: <001101cc1a22$d100e9d0$7302bd70$@com> References: <001101cc1a22$d100e9d0$7302bd70$@com> Message-ID: <259132.9447.qm@web65711.mail.ac4.yahoo.com> For tissues containing bone, or bone specimens I always used the commercial brand RDO.? For bone marrow biopsies I used EDTA (prepared in the lab). Ren? J. From: Cynthia Pyse To: 'Histonet' Sent: Tuesday, May 24, 2011 10:56 AM Subject: [Histonet] decal Hello Histonetters What is everyone using to decal specimens. I haven't had to use it in a few years, before that I just purchased formic acid and made a 10% solution. Just wanted to see if the criteria has changes over the years. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arvidsonkristen <@t> yahoo.com Tue May 24 11:01:03 2011 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Tue May 24 11:01:07 2011 Subject: [Histonet] Processor power supply Back-up Message-ID: <2613.13896.qm@web65711.mail.ac4.yahoo.com> Does anyone have any suggestions on power supply back-up for Leica ASP 300?? We are an independant lab and are not hooked up to back-up if we have an outage.? Thought it might be a good idea :) ? Thanks. From chak_bou <@t> yahoo.com Tue May 24 11:34:55 2011 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Tue May 24 11:34:58 2011 Subject: [Histonet] Coronavirus antibody Message-ID: <425068.63659.qm@web161818.mail.bf1.yahoo.com> Hi Histonetters, We are interesting in doing immunostain on ferret tissues that have been fixed in formalin and embedded in paraffin. I am just wondering if anyone used this antibody in their lab, if so, can you please share with us your staining protocol? antigen retrieval protocol as well? Your help will be very much appreciated. Thank you Chakib HTL(ASCP) Histology lab MIT From Vickroy.Jim <@t> mhsil.com Tue May 24 11:46:17 2011 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue May 24 11:46:22 2011 Subject: [Histonet] Pathology Transmission Electron Micrscope Message-ID: <24A4826E8EF0964D86BC5317306F58A55DF5BEC1C4@mmc-mail.ad.mhsil.com> We are in the market to purchase a new TEM. Our old Hitachi instrument is 31 years old and is getting tired. The use for transmission em in pathology has somewhat diminished in our hospital environment. We still however use TEM routinely on all renal biopsies and nerve biopsies. Has anyone priced TEM scopes for pathology usage lately. In the past there have not been many options for a TEM that was for pathology usage verses one for research. Can anyone steer me in the right direction. Of course cost has become a major issue also so we are trying to make the best choice for our usage. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From candice_camille <@t> yahoo.com Tue May 24 12:04:01 2011 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Tue May 24 12:04:05 2011 Subject: [Histonet] WEt mount Message-ID: <6598.74839.qm@web125408.mail.ne1.yahoo.com> Hi guys. I?am new to this.. Can someone explain to me how to wet mount properly. What is it? ?I remain yours truely, Candice Camille From shive003 <@t> umn.edu Tue May 24 12:14:04 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue May 24 12:14:08 2011 Subject: [Histonet] Coronavirus antibody References: <425068.63659.qm@web161818.mail.bf1.yahoo.com> Message-ID: <095E8A2F8BC0485198B9EF012EB4321F@auxs.umn.edu> Hello, We have successfully stained ferret tissue using an antibody to Feline Infectious Peritonitis Virus (which also cross-reacts with swine Transmissible Gastroenteritis Virus). We buy our antibody from Custom Monoclonals, Inc. and use heat-induced epitope retrieval (pressure cooker and target retrieval solution). If you would like more details than this, I can send an uncontrolled copy of our SOP to you. Best regards, Jan Shivers Senior Scientist IHC/Histology/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "Chakib Boussahmain" To: Sent: Tuesday, May 24, 2011 11:34 AM Subject: [Histonet] Coronavirus antibody > Hi Histonetters, > We are interesting in doing immunostain on ferret tissues that have been > fixed in formalin and embedded in paraffin. I am just wondering if anyone > used this antibody in their lab, if so, can you please share with us your > staining protocol? antigen retrieval protocol as well? > Your help will be very much appreciated. > Thank you > Chakib HTL(ASCP) > Histology lab > MIT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kblack <@t> digestivehlth.com Tue May 24 12:18:30 2011 From: kblack <@t> digestivehlth.com (Konni Black) Date: Tue May 24 12:18:39 2011 Subject: [Histonet] part-time position Portland Oregon Message-ID: <5C7AAEA2A04945CEB3A08D07A4CC1746@digestivehlth.com> A friend has asked me to post an opening for an HT or HTL partime position. It would be about 20 hours per week in Portland, Oregon. If you have an interest, you can contact me via e-mail. kblack@digestivehlth.com Thank you, Konni Black From madary <@t> verizon.net Tue May 24 13:47:59 2011 From: madary <@t> verizon.net (madary@verizon.net) Date: Tue May 24 13:48:11 2011 Subject: [Histonet] EM purchase Message-ID: <231125295.540119.1306262879499.JavaMail.root@vznit170078> Fortunate enough to purchase and use several TEM and SEM's over the ye= ars, I always found Zeiss to have the best prices and products for TEM and = Hitachi for the SEM's. Last one I bought was 200K plus. Good Luck and= let me know if you know of anyone looking for an EM tech in the dc area. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC Joni Madary, PhD(in life)May 2= 4, 2011 12:01:20 PM, histonet@lists.utsouthwestern.edu wrote: Send Histonet= mailing list submissions to histonet@lists.utsouthwestern.edu To= subscribe or unsubscribe via the World Wide Web, visit http://lists.uts= outhwestern.edu/mailman/listinfo/histonet or, via email, send a message = with subject or body 'help' to histonet-request@lists.utsouthwestern.edu= You can reach the person managing the list at histonet-owner@lis= ts.utsouthwestern.edu When replying, please edit your Subject line s= o it is more specific than "Re: Contents of Histonet digest..." <= BR>Today's Topics: 1. RE: p63 (McMahon, Loralee A) 2. Re: Histone= t Digest, Vol 90, Issue 28 (boneslides@aol.com) 3. RE: p63 (Bell, Lynne)= 4. Re: Freezing bath solutions (Robert Richmond) 5. Jones stain boo = boo (Norton, Sally) 6. Re: Re: Freezing bath solutions (mwhite@mcleodhea= lth.org) 7. IHC Control Tissues (Goins, Tresa) 8. FISH (wassan alkadh= umi) 9. AW: [Histonet] FISH (Gudrun Lang) 10. Las Vegas Position (Hal= e, Meredith) 11. RE: p63 (Dessoye, Michael J) 12. Thank you (Margaret= Blount) 13. Re: Re: Freezing bath solutions (abright@brightinstruments.= com) 14. decal (Cynthia Pyse) 15. Re: decal (Rene J Buesa) 16. Pro= cessor power supply Back-up (kristen arvidson) 17. Coronavirus antibody = (Chakib Boussahmain) 18. Pathology Transmission Electron Micrscope (Vick= roy, Jim) ------------------------------------------------------= ---------------- Message: 1 Date: Mon, 23 May 2011 13:33:27 -0400= From: "McMahon, Loralee A" Subj= ect: [Histonet] RE: p63 To: Joanne Clark , "histonet= @lists.utsouthwestern.edu" Messag= e-ID: Content-Type: text/plain; charset=3D"us-ascii" Bioca= re Medical Loralee McMahon, HTL (ASCP) Immunohistochemistry Super= visor Strong Memorial Hospital Department of Surgical Pathology (5= 85) 275-7210 ________________________________________ From: histonet-= bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu= ] On Behalf Of Joanne Clark [jclark@pcnm.com] Sent: Monday, May 23, 2011= 12:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p= 63 I need to find a source for p63 antibody. Where does everyone pur= chase theirs from? Joanne Clark,HT Pathology Consultants of New M= exico _______________________________________________ Histonet ma= iling list Histonet@lists.utsouthwestern.edu http://lists.utsouthwest= ern.edu/mailman/listinfo/histonet ------------------------------= Message: 2 Date: Mon, 23 May 2011 13:41:36 -0400 (EDT) From: = boneslides@aol.com Subject: [Histonet] Re: Histonet Digest, Vol 90, Issu= e 28 To: histonet@lists.utsouthwestern.edu Message-ID: <8CDE787CC3= 64FAB-17A0-7F431@webmail-d058.sysops.aol.com> Content-Type: text/plai= n; charset=3D"utf-8" Dear Miss Margaret Blount: Congratulatio= ns on your retirement!! May you enjoy good health and many years doing the = things you WANT to do!! God Bless! -----Origi= nal Message----- From: histonet-request To: histonet Sent: Mo= n, May 23, 2011 1:00 pm Subject: Histonet Digest, Vol 90, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwe= stern.edu To subscribe or unsubscribe via the World Wide Web, visit h= ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet r, via email, s= end a message with subject or body 'help' to histonet-request@lists.utso= uthwestern.edu You can reach the person managing the list at histonet= -owner@lists.utsouthwestern.edu When replying, please edit your Subject = line so it is more specific han "Re: Contents of Histonet digest..." = oday's Topics: 1. Immuno controls (Carrie Disbrow) 2. This is goo= dbye (Margaret Blount) 3. In vitro mouse embryo culture (Carmen Maria Ga= rcia Pascual) 4. Re: Immuno controls (Rene J Buesa) 5. RE: Histonet D= igest, Vol 90, Issue 27 (Barone, Carol ) 6. RE: Histonet Digest, Vol 90,= Issue 27 (Barone, Carol ) 7. Freezing bath solutions (Scott, Allison D)= 8. p63 (Joanne Clark) ------------------------------------------= --------------------------- Message: 1 ate: Sun, 22 May 2011 22:00:05= -0400 rom: Carrie Disbrow ubject: [Histonet] Immu= no controls o: essage-ID: ontent-Type: text/plain; charset= =3D"iso-8859-1" i all, fter consideration I disagree with the= consensus and also agree with Curt's athologist. More supplies and lab= or will be involved as cutting more controls ill be neccessay to meet t= he requests of the pathologist. The patient tissue is ut on two control= s slides. One slide is used as the negative control and has oth the pat= ient tissue and the known positive control which should both be egative= after the run. The other slide with the known positive control and the atient tissue in question is also stained with the positive control staini= ng ositive. hank you! ------------------------------ Messa= ge: 2 ate: Mon, 23 May 2011 09:04:54 +0100 rom: "Margaret Blount" ubject: [Histonet] This is goodbye o: essage-ID: ontent-Type: text/plain; charset= =3D"US-ASCII" To everyone in histoland, This is my last full week= at work. From next Tuesday I will be retiring rom my job at Cambridge U= niversity. I have thoroughly enjoyed my chosen rofession as a research t= echnician specialising in histology. I realise hat because I have always= worked in research my experience is somewhat imited: I haven't been ask= ed for the same range of techniques that you ind in a routine clinical l= ab. However, I have enjoyed my work and have earnt and devised some tech= niques for a range of interesting samples. ow it's time to learn somethi= ng new! I hope to spend more time with my lace pillow and learn to = do it roperly, maybe learn to do Northamptonshire lace which I believe i= s a ariation on Bucks point. (I come originally from Northants) I have h= ad go at Bedfordshire and will learn more, it is very pretty. I also pla= n o travel; my next trip is to New Zealand in November. I won't = go on. I just want to say this: You are all great guys and irls; you sha= re your experience and friendship with great generosity, hich I have alw= ays felt is what a scientist should do. Thank you for our comradeship an= d help over the years. Maybe I'll get to meet some of ou by chance durin= g my travels. Best wishes to you all and a great big Thank You, <= BR>From Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science<= BR>Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 ------------------------------ Messa= ge: 3 ate: Mon, 23 May 2011 10:35:20 +0200 (CEST) rom: "Carmen Maria = Garcia Pascual" ubject: [Histonet] In vitro mouse= embryo culture o: histonet@lists.utsouthwestern.edu essage-ID: <6= 395165382carmaga6@uv.es> ontent-Type: text/plain; charset=3D"ISO-8859= -1" Good Moorning to everyone: I am Carmen Garcia a PhD student f= rom Valencia, we are know interesting n make in vitro culture of mouse = embryos to do toxicity tests and in ur lab we have not experience. some= one can give me some advise? or ecommend me some bibliography? ow lo= ng can I have the embryos in culture? hank you so much!!! Best regard= s, armen ------------------------------ Message: 4 ate:= Mon, 23 May 2011 07:48:50 -0700 (PDT) rom: Rene J Buesa ubject: Re: [Histonet] Immuno controls o: Carrie Disbrow , "histonet@lists.utsouthwestern.edu" essage-ID: <87688.31423.qm@web65714.mail.ac4.yahoo.co= m> ontent-Type: text/plain; charset=3Diso-8859-1 Also Sprach Zarat= hustra!!!! en=C3=AF=C2=BF=C2=BD J. From: Carrie Disbrow o: histonet@lists.utsouthwestern.edu ent: Sunday, May 22, 2011 = 10:00 PM ubject: [Histonet] Immuno controls i all, fter co= nsideration I disagree with the consensus and also agree with Curt's at= hologist. More supplies and labor will be=C3=AF=C2=BF=C2=BD involved as=C3= =AF=C2=BF=C2=BD cutting more controls ill be neccessay to meet the requ= ests of the pathologist. The patient tissue is ut on two controls slide= s. One slide is used as the negative control and has oth the patient ti= ssue and the known positive control which should both be egative after = the run. The other slide with the known positive control and the atient= tissue in question is also stained with the positive control staining = ositive. hank you! =C3=AF=C2=BF=C2=BD=C3=AF=C2=BF=C2=BD=C3=AF=C2=BF= =C2=BD =C3=AF=C2=BF=C2=BD=C3=AF=C2=BF=C2=BD=C3=AF=C2=BF=C2=BD =C3=AF=C2=BF= =C2=BD=C3=AF=C2=BF=C2=BD=C3=AF=C2=BF=C2=BD =C3=AF=C2=BF=C2=BD =C3=AF=C2=BF= =C2=BD=C3=AF=C2=BF=C2=BD=C3=AF=C2=BF=C2=BD =C3=AF=C2=BF=C2=BD=C3=AF=C2=BF= =C2=BD=C3=AF=C2=BF=C2=BD =C3=AF=C2=BF=C2=BD _______________________________= ________________ istonet mailing list istonet@lists.utsouthwestern.ed= u ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------= -------------------- Message: 5 ate: Mon, 23 May 2011 11:02:16 -0400<= BR>rom: "Barone, Carol " ubject: [Histonet] RE: Hi= stonet Digest, Vol 90, Issue 27 o: essage-ID: ontent-Type: text/plain; charset=3D"us-ascii" -----Original Me= ssage----- rom: histonet-bounces@lists.utsouthwestern.edu mailto:hist= onet-bounces@lists.utsouthwestern.edu] On Behalf Of istonet-request@list= s.utsouthwestern.edu ent: Sunday, May 22, 2011 1:01 PM o: histonet@li= sts.utsouthwestern.edu ubject: Histonet Digest, Vol 90, Issue 27 Send= Histonet mailing list submissions to To subscribe or unsubscribe vi= a the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/list= info/histonet r, via email, send a message with subject or body 'help' t= o histonet-request@lists.utsouthwestern.edu You can reach the person = managing the list at histonet-owner@lists.utsouthwestern.edu When rep= lying, please edit your Subject line so it is more specific than Re: Con= tents of Histonet digest..." oday's Topics: 1. to Rene J Buesa (M= axim Peshkov) ------------------------------------------------------= --------------- Message: 1 ate: Sun, 22 May 2011 13:43:07 +0400 ro= m: Maxim Peshkov ubject: [Histonet] to Rene J Buesao: Rene J Buesa c: histonet@lists.utsouthwestern.e= du essage-ID: <1585823684.20110522134307@mail.ru> ontent-Type: = text/plain; charset=3Dwindows-1251 I am sorry, that uses histonet for pe= rsonal goals. Dear Rene! ast 3 my answers for you are returned back t= o me. our e-mails I got successfully. upport service of mail.ru wrote= that problem is at yahoo.com server. lease check your postmaster servic= e. Here is my post-address: axim Peshkov, l. Mikhailovskaya, 90-A,= 47909, aganrog, ussia. Sincerely, axim. mailto:Maxim_71@= mail.ru ----------------------------- _______________________= ________________________ istonet mailing list istonet@lists.utsouthwe= stern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet En= d of Histonet Digest, Vol 90, Issue 27 *********************************= ****** ------------------------------ Message: 6 ate: Mon, 23 = May 2011 11:15:34 -0400 rom: "Barone, Carol " u= bject: [Histonet] RE: Histonet Digest, Vol 90, Issue 27 o: essage-ID: ontent-Type: text/plain; charset=3D"us-ascii"= Histonetters: Needed to stain xenograft with "Bunny"-IgG-direct, thatad already been in formailin two weeks (was FFPE). Normally would do n= a frozen 1:40? ....We have background everywhwere on first un...suggest= ions? Neg is also shining??? Crazy researcher's! Got to ove them...when = will they learn to ask first? We are diluting primary in plan two, and w= ill stain over-night at 4 egrees....but, this thing is lit-up like the S= UN! was expecting low signal? Go figure? ----Original Message---= -- rom: histonet-bounces@lists.utsouthwestern.edu mailto:histonet-bou= nces@lists.utsouthwestern.edu] On Behalf Of istonet-request@lists.utsout= hwestern.edu ent: Sunday, May 22, 2011 1:01 PM o: histonet@lists.utso= uthwestern.edu ubject: Histonet Digest, Vol 90, Issue 27 Send Histone= t mailing list submissions to histonet@lists.utsouthwestern.edu To su= bscribe or unsubscribe via the World Wide Web, visit http://lists.utsout= hwestern.edu/mailman/listinfo/histonet r, via email, send a message with= subject or body 'help' to histonet-request@lists.utsouthwestern.edu = You can reach the person managing the list at histonet-owner@lists.utsou= thwestern.edu When replying, please edit your Subject line so it is more= specific than Re: Contents of Histonet digest..." oday's Topics:= 1. to Rene J Buesa (Maxim Peshkov) -----------------------------= ---------------------------------------- Message: 1 ate: Sun, 22 May = 2011 13:43:07 +0400 rom: Maxim Peshkov ubject: [Hi= stonet] to Rene J Buesa o: Rene J Buesa c: histon= et@lists.utsouthwestern.edu essage-ID: <1585823684.20110522134307@mai= l.ru> ontent-Type: text/plain; charset=3Dwindows-1251 I am sorry, = that uses histonet for personal goals. Dear Rene! ast 3 my answers fo= r you are returned back to me. our e-mails I got successfully. upport= service of mail.ru wrote that problem is at yahoo.com server. lease che= ck your postmaster service. Here is my post-address: axim Peshkov, l. Mikhailovskaya, 90-A, 47909, aganrog, ussia. Sincerely, = axim. mailto:Maxim_71@mail.ru -----------------------------______________________________________ _________ istonet mailing lististonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailma= n/listinfo/histonet End of Histonet Digest, Vol 90, Issue 27 ********= ******************************* ------------------------------ Me= ssage: 7 ate: Mon, 23 May 2011 11:22:24 -0500 rom: "Scott, Allison D"= ubject: [Histonet] Freezing bath solutions= o: essage-ID: <1872B4A455B= 7974391609AD8034C79FC026DFDD9@LBEXCH01.hchd.local> ontent-Type: text/= plain; charset=3D"us-ascii" Hello to all in histoland. What type of solu= tions are being used if any o freezze your chucks for frozen sections. O= ur sister hospital is sing methyl butane and there was a flash fire last= week. We use the ame solution. Any suggestions will be greatly apprecia= ted. Allison Scott HT(ASCP) istology Supervisor BJ Hospital ONF= IDENTIALITY NOTICE: f you have received this e-mail in error, please imm= ediately notify the ender by return e-mail and delete this e-mail and an= y attachments from our computer system. To the extent the informatio= n in this e-mail and any attachments contain rotected health informatio= n as defined by the Health Insurance Portability nd Accountability Act = of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 64; or Chapter 181,= Texas Health and Safety Code, it is confidential and/or rivileged. Thi= s e-mail may also be confidential and/or privileged under exas law. The= e-mail is for the use of only the individual or entity named bove. If = you are not the intended recipient, or any authorized epresentative of = the intended recipient, you are hereby notified that any eview, dissemi= nation or copying of this e-mail and its attachments is trictly prohibi= ted. ------------------------------ Message: 8 ate: Mon, 23 Ma= y 2011 16:31:08 +0000 rom: Joanne Clark ubject: [Hi= stonet] p63 o: "histonet@lists.utsouthwestern.edu" essage-ID: <0494A7D4E8CC254EA2FB81464982E3786DCA= 72@S10MAILD001N1.SH10.lan> ontent-Type: text/plain; charset=3D"us-asc= ii" I need to find a source for p63 antibody. Where does everyone purcha= se theirs rom? Joanne Clark,HT athology Consultants of New Mexico= ------------------------------ _________________________________= ______________ istonet mailing list istonet@lists.utsouthwestern.edu<= BR>ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histo= net Digest, Vol 90, Issue 28 * --------------------------= ---- Message: 3 Date: Mon, 23 May 2011 13:45:50 -0400 From: "B= ell, Lynne" Subject: [Histonet] RE: p63 To: 'Jo= anne Clark' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; char= set=3D"us-ascii" I believe the only place you can buy p63 is through= Biocare, www.biocare.net. Lynne Bell, HT (ASCP) Histology Te= am Leader 802-371-4923 ------------------------------ = Message: 4 Date: Mon, 23 May 2011 14:12:37 -0400 From: Robert Ric= hmond Subject: [Histonet] Re: Freezing bath solut= ions To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset= =3DISO-8859-1 Allison Scott after a flash fire with the stuff asks a= bout alternatives to 2-methylbutane for freezing specimens. There= is indeed a safe alternative, though I haven't had a chance to try it. = I've posted about it on Histonet more than once - here's a copy of an ea= rlier post of mine: 3M(tm) Novec(tm) Engineered Fluid HFE-7100 Th= is product belongs to a class of fluorocarbons called "segregated hydrof= luoroethers (HFE's)" According to various MSDS, HFE-7100 is methyl no= nafluoroisobutyl ether C4F9-O-CH3 It melts and freezes at -135 C, so = that it would remain liquid in the Histobath. (It would freeze solid in = liquid nitrogen, however.) It boils at 60 C., and is listed as non-flamm= able. It has almost no vapor pressure at freezing temperatures. It co= st around $230 a gallon a few years ago. Bob Richmond Samurai Pat= hologist Knoxville TN ------------------------------ <= BR>Message: 5 Date: Mon, 23 May 2011 11:28:28 -0700 From: "Norton, Sa= lly" Subject: [Histonet] Jones stain= boo boo To: Message-ID: <1= 6E0693C7018C245959AC729FE66EDE52DBECB@s107.childrens.sea.kids> Conten= t-Type: text/plain; charset=3D"us-ascii" I accidentally put my renal= slides in the Methanimine Silver (after Periodic Acid) before putting t= hem in the Thiosemicarbizine. Slides take on a yellow hue which makes th= e H&E counterstain muddy. Is there a way to correct this? Thank you, Sally Norton Histotech CONFIDENTIALITY NOTICE: This e-mail message, including any attach= ments, is for the sole use of the intended recipient(s) and may contain con= fidential and privileged information protected by law. Any unauthorized rev= iew, use, disclosure or distribution is prohibited. If you are not the inte= nded recipient, please contact the sender by reply e-mail and destroy all c= opies of the original message. -----------------------------= - Message: 6 Date: Mon, 23 May 2011 14:46:29 -0400 From: mwhit= e@mcleodhealth.org Subject: Re: [Histonet] Re: Freezing bath solutionsTo: Robert Richmond Cc: histonet@lists.utsouthw= estern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: = Content-Type: text/plain; charset=3D"us-ascii" Allison and = Dr. Richmond: We are using Novec 7000 successfully in our freezing b= ath and have been doing so for about 6 months with no major problems. It does have some annoying traits ; however the safety benefits outwei= gh those issues , in my opinion. The issues are: a little more time requ= ired for specimen to freeze, tissues tending to float in the solution, a= nd evaporation. I worried that the evaporation would be a big problem , = but it has not .My techs discovered through experimentation that by lowe= ring the tissue slowly into the fluid, it would stop the flotation probl= em. Overall, I would recommend it. 3M has been very helpful to us. (= I'm not being paid in any way to endorse this!!) See below for 3M contac= t information. Additionally, through earlier Histonet inquiries, = I was told about a freezing bath for those of you who still use the old = Histobath that is no longer made. If anyone wants information about it, = It's on www.ftssystems.com and the item is called a "Mult-Cool Bath". We= don't have one , but it looks like it could be a suitable replacement.<= BR> Here's our purchasing contact person at 3M: Christina Cerecedes1-888-509-5224 Melanie S. White, MT(ASCP) Laboratory Superv= isor, Systems/Anatomic Pathology McLeod Regional Medical Center Flore= nce, SC (843) 777-2072 NOTICE: This e-mail message and al= l attachments transmitted with it may contain legally PRIVILEGED and CO= NFIDENTIAL information intended solely for the use of the addressee. If= the reader of this message is not the intended recipient, you are here= by notified that any reading, dissemination, distribution, copying, or = other use of this message or its attachments is strictly prohibited. If= you have received this message in error, please notify the sender = immediately and/or notify the postmaster (postmaster@mcleodhealth.org), and= delete this message and all copies and backups thereof. Thank You. = ------------------------------ Message: 7 Date: Mon, = 23 May 2011 18:56:05 +0000 From: "Goins, Tresa" Subje= ct: [Histonet] IHC Control Tissues To: "histonet@lists.utsouthwestern.ed= u" Message-ID: Content-Type: text/pla= in; charset=3D"us-ascii" If you haven't made up your mind yet on thi= s issue, here are a couple of references that may - or may not - help: <= BR>Veterinary Diagnostics: http://www.ncbi.nlm.nih.gov/sites/entrez?= orig_db=3DPubMed&db=3Dpubmed&cmd=3DSearch&term=3D%22Journal%20o= f%20veterinary%20diagnostic%20investigation%20%3A%20official%20publ ication%= 20of%20the%20American%20Association%20of%20Veterinary%20Laboratory% 20Diagno= sticians%2C%20Inc%22%5BJour%5D%20AND%2020%5Bvolume%5D%20AND%20393%5 Bpage%5D= %20AND%202008%5Bpdat%5D FDA F.20 Quality Control: = http://www.fda.gov/downloads/MedicalDevices/DeviceRegulationandGuid ance/Gui= danceDocuments/ucm094015.pdf Tresa Goins Histopathology Super= visor Department of Livestock Bozeman, Montana -------= ----------------------- Message: 8 Date: Mon, 23 May 2011 12:23:5= 4 -0700 (PDT) From: wassan alkadhumi Subject:= [Histonet] FISH To: h n Message-= ID: <539226.53686.qm@web45202.mail.sp1.yahoo.com> Content-Type: te= xt/plain; charset=3Diso-8859-1 Dear members We jus= t started doing FISH using HER2 FISH pharma Dx Dako kit, Code number K5331 = on malignant breast tissues. I was wondering what is the succe= ss ratio or percentage for say a one run?? my hospital is the firs= t in our region to start FISH and I am the first to run it. = I have no experience with the hybridizer but by reading the&= nbsp;hybridizer manual and the kit insert i discover it is user friendly. <= BR>I run the first batch (six slides only) yesterday. Continue an= d finish it today. only three of the slides worked. I will star= t a new batch next week and I appreciate any help you may offer to avoi= d inaccurate result. Thank you Wassan Histotec= hnician Shorsh General Hospital North of Iraq ----------------= -------------- Message: 9 Date: Mon, 23 May 2011 22:15:37 +0200From: "Gudrun Lang" Subject: AW: [Histonet] FISH T= o: "'wassan alkadhumi'" Cc: histonet@lists.utsou= thwestern.edu Message-ID: <00DDDF450F674127ACFF7D694679751A@dielangs.= at> Hi Wassan, you may depart the FISH procedure in a tissue-d= ependant first part and a probe-dependant second part. The first part= comprises the pretreatment of the tissue to retrieve and permeabilises = it. The amount of pretreatment is dependant on the fixation-duration of = the tissue. The longer the fixation - the higher the temperature of the = buffer, the longer the incubation time in the buffer, the longer the inc= ubation time in the protease. Troubleshooting in this part: To= o low temperature of some slides because of inconsistent temperature in = the waterbath. Tissueslides mounted on the glass-slides in different hei= ghts. Too short protease-incubation in relation to the fixation time. Us= ually nuclei can be digested until you see the first tiny holes. Underdi= gested nuclei are dense and look grey in the triplefilter. If the nuc= lei are overdigested, they get bigger holes and fuzzy borders. Different= staining results due to variing fixation-times. The second part= comprises the stringent wash. The temperature, buffer-quality, duration= are probe-depending and are usually provided in the kit-handbook. Troubleshooting second part: Too high temp leads to loosing the bound = probe. Too low temp leads to high background because of unspecifc bound = probes. With your problem I would try to prolong the digestion in 10= min steps, until a good result is found. Hint: standardization of fi= xation is the crucial point. In our lab the tumor-block is immediately f= ixed in a cassette as a FISH-block. So it will be fixed very well and wi= thstands the harsh treatment. The disadvantage is stronger autofluoresce= nce. A further hint: be sure, that the gum around the coverslip is harde= ned before starting the hybridizer-protocol. 10 min drying time. = I hope this helps Gudrun Lang -----Urspr=C3=BCngliche= Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailt= o:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von wassan alkad= humi Gesendet: Montag, 23. Mai 2011 21:24 An: h n Betreff: [Histon= et] FISH Dear members We just started doing FISH u= sing HER2 FISH pharma Dx Dako kit, Code number K5331 on malignant bre= ast tissues. I was wondering what is the success ratio or percenta= ge for say a one run?? my hospital is the first in our region&nb= sp;to start FISH and I am the first to run it. I have no experien= ce with the hybridizer but by reading the hybridizer = manual and the kit insert i discover it is user friendly. I run the f= irst batch (six slides only) yesterday. Continue and finish = it today. only three of the slides worked. I will start a new batc= h next week and I appreciate any help you may offer to avoid inaccura= te result. Thank you Wassan Histotechnician Shorsh General Hospital North of Iraq ______________________________= _________________ Histonet mailing list Histonet@lists.utsouthwestern= .edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mo= n, 23 May 2011 16:53:16 -0500 From: "Hale, Meredith" = Subject: [Histonet] Las Vegas Position To: Message-ID: <6F33D8418806044682A391273399860F08374FCA@s= -irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; = charset=3D"us-ascii" Great opportunity for a Histotechnician= in a brand new laboratory! We are a 5 physician gastroenterology practi= ce located in Las Vegas, NV looking for a certified HT or HTL. Candidate= must meet CLIA grossing requirements. The candidate will be responsible= for routine histology duties. This is a part-time position that offers = a competitive salary and flexible hours. Interested applicants should co= ntact Meredith Hale, phone (214)596-2219 or e-mail mhale@carisls.com = Meredith Hale HT (ASCP) CM Operations Liaison Di= rector and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219<= BR> cell: 469-648-8253 fax: 214-596-7095 mhale@carisls.com= = ------------------------------ Message: 11 Date: Tue,= 24 May 2011 07:14:23 -0400 From: "Dessoye, Michael J" Subject: RE: [Histonet] p63 To: "Joanne Clark" = , Message-ID: Content-Type: text/p= lain; charset=3D"iso-8859-1" Biocare has a very good, and fairly ine= xpensive, p63 that we have had good results with: http://biocare.net/produc= ts/antibodies/p/163/ ________________________________ From: J= oanne Clark [mailto:jclark@pcnm.com] Sent: Mon 5/23/2011 12:31 PM To:= histonet@lists.utsouthwestern.edu Subject: [Histonet] p63 I need to find a source for p63 antibody. Where does everyone purchase th= eirs from? Joanne Clark,HT Pathology Consultants of New Mexico _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ = _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are c= onfidential and intended solely for the use of the individual or entity = to whom they are addressed. If you have received this email in error = please notify the originator of the message. This footer also confirms t= hat this email message has been scanned for the presence of computer vir= uses. Any views expressed in this message are those of the individua= l sender, except where the sender specifies and with authority, state= s them to be the views of Wyoming Valley Health Care System. Scannin= g of this message and addition of this footer is performed by Websense E= mail Security software in conjunction with virus detection software. ------------------------------ Message: 12 Date: Tue= , 24 May 2011 12:14:02 +0100 From: "Margaret Blount" Subject: [Histonet] Thank you To: Message-ID: Content-Type: text/plain; charset=3D"US-ASCII" Hello everyone! Thank you all for your good wishes on my r= etirement. I knew you were all great people: you have just proved it big= time! Thank you all from the bottom of my heart. Keep smiling! Much love Margaret = ------------------------------ Message: 13 Date: Tue, 24 = May 2011 13:03:21 +0000 From: abright@brightinstruments.com Subject: = Re: [Histonet] Re: Freezing bath solutions To: mwhite@mcleodhealth.org,<= BR>histonet-bounces@lists.utsouthwestern.edu, "Robert Richmond" Cc: Histonet Messa= ge-ID: <757762693-1306242203-cardhu_decombobulator_blackberry.rim.net= -1362851250-@b15.c2.bise7.blackberry> Content-Type: text/plain; c= harset=3D"Windows-1252" Melanie, In our Clini-RF rapid freezer we= have a device for keeping tissue submerged in Novec 7000. Regards <= BR> Alan Bright www.brightinstruments.com Sent from my BlackBerry= =C2=AE wireless device -----Original Message----- From: mwhite@mc= leodhealth.org Sender: histonet-bounces@lists.utsouthwestern.edu Date= : Mon, 23 May 2011 14:46:29 To: Robert RichmondCc: ; Subject: Re: [Histonet] Re: Freezing bath solutions A= llison and Dr. Richmond: We are using Novec 7000 successfully in our= freezing bath and have been doing so for about 6 months with no major p= roblems. It does have some annoying traits ; however the safety bene= fits outweigh those issues , in my opinion. The issues are: a little mor= e time required for specimen to freeze, tissues tending to float in the = solution, and evaporation. I worried that the evaporation would be a big= problem , but it has not .My techs discovered through experimentation t= hat by lowering the tissue slowly into the fluid, it would stop the flot= ation problem. Overall, I would recommend it. 3M has been very helpf= ul to us. (I'm not being paid in any way to endorse this!!) See below fo= r 3M contact information. Additionally, through earlier Histonet = inquiries, I was told about a freezing bath for those of you who still u= se the old Histobath that is no longer made. If anyone wants information= about it, It's on www.ftssystems.com and the item is called a "Mult-Coo= l Bath". We don't have one , but it looks like it could be a suitable re= placement. Here's our purchasing contact person at 3M: Christina = Cerecedes 1-888-509-5224 Melanie S. White, MT(ASCP) Labora= tory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Cent= er Florence, SC (843) 777-2072 NOTICE: This e-mail mes= sage and all attachments transmitted with it may contain legally PRIVIL= EGED and CONFIDENTIAL information intended solely for the use of the ad= dressee. If the reader of this message is not the intended recipient, y= ou are hereby notified that any reading, dissemination, distribution, c= opying, or other use of this message or its attachments is strictly pro= hibited. If you have received this message in error, please notify the = sender immediately and/or notify the postmaster (postmaster@mcleodhealt= h.org), and delete this message and all copies and backups thereof. Thank Y= ou. _______________________________________________ Histonet = mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwe= stern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING= -LINKS ------------------------------------------------------ Tea= ch SpamSniper if this mail (ID 01EL6KSKd) is spam: Spam: http://admin.sp= amsniper.co.uk/canit/b.php?i=3D01EL6KSKd&m=3Dce12d22f644f&t=3D20110 = 523&c=3Ds Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=3D01= EL6KSKd&m=3Dce12d22f644f&t=3D20110523&c=3Dn Forget vote: htt= p://admin.spamsniper.co.uk/canit/b.php?i=3D01EL6KSKd&m=3Dce12d22f64 4f&a= mp;t=3D20110523&c=3Df ----------------------------------------------= -------- END-ANTISPAM-VOTING-LINKS --------------------------= ---- Message: 14 Date: Tue, 24 May 2011 10:56:52 -0400 From: "= Cynthia Pyse" Subject: [Histonet] decal To: "'H= istonet'" Message-ID: <001101cc1a= 22$d100e9d0$7302bd70$@com> Content-Type: text/plain; charset=3D"us-as= cii" Hello Histonetters What is everyone using to decal speci= mens. I haven't had to use it in a few years, before that I just purchas= ed formic acid and made a 10% solution. Just wanted to see if the criter= ia has changes over the years. Thanks in advance for the responses. <= BR> Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Superviso= r X-Cell Laboratories e-mail cpyse@x-celllab.com <= BR> ------------------------------ Message: 15 Dat= e: Tue, 24 May 2011 08:50:13 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] decal To: Cynthia Pyse , 'Histonet' Message-ID: &= lt;259132.9447.qm@web65711.mail.ac4.yahoo.com> Content-Type: text/pla= in; charset=3Diso-8859-1 For tissues containing bone, or bone specim= ens I always used the commercial brand RDO. For bone marrow biopsi= es I used EDTA (prepared in the lab). Ren=C3=A9 J. From: Cynthia = Pyse To: 'Histonet' Sent: Tuesday, May 24, 2011 10:56 AM Subject: [Histonet] decal<= BR> Hello Histonetters What is everyone using to decal specimens.= I haven't had to use it in a few years, before that I just purchased fo= rmic acid and made a 10% solution. Just wanted to see if the criteria ha= s changes over the years. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor = X-Cell Laboratories e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing l= ist Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu= /mailman/listinfo/histonet ------------------------------ Mes= sage: 16 Date: Tue, 24 May 2011 09:01:03 -0700 (PDT) From: kristen ar= vidson Subject: [Histonet] Processor power s= upply Back-up To: histonet Messag= e-ID: <2613.13896.qm@web65711.mail.ac4.yahoo.com> Content-Type: te= xt/plain; charset=3Diso-8859-1 Does anyone have any suggestions on p= ower supply back-up for Leica ASP 300? We are an independant lab and = are not hooked up to back-up if we have an outage. Thought it might b= e a good idea :) Thanks. ------------------------------= Message: 17 Date: Tue, 24 May 2011 09:34:55 -0700 (PDT) From:= Chakib Boussahmain Subject: [Histonet] Coronavirus= antibody To: histonet@lists.utsouthwestern.edu Message-ID: <42506= 8.63659.qm@web161818.mail.bf1.yahoo.com> Content-Type: text/plain; ch= arset=3Dus-ascii Hi Histonetters, We are interesting in doing imm= unostain on ferret tissues that have been fixed in formalin and embedded in= paraffin. I am just wondering if anyone used this antibody in their lab, i= f so, can you please share with us your staining protocol? antigen retrieva= l protocol as well? Your help will be very much appreciated. Thank yo= u Chakib HTL(ASCP) Histology lab MIT ----------------------= -------- Message: 18 Date: Tue, 24 May 2011 11:46:17 -0500 Fro= m: "Vickroy, Jim" Subject: [Histonet] Pathology = Transmission Electron Micrscope To: "histonet@lists.utsouthwestern.edu"<= BR> Message-ID: <24A4826E8EF096= 4D86BC5317306F58A55DF5BEC1C4@mmc-mail.ad.mhsil.com> Content-Type: tex= t/plain; charset=3D"us-ascii" We are in the market to purchase a= new TEM. Our old Hitachi instrument is 31 years old and is getting tired. = The use for transmission em in pathology has somewhat diminished in our hos= pital environment. We still however use TEM routinely on all renal biopsies= and nerve biopsies. Has anyone priced TEM scopes for pathology usage latel= y. In the past there have not been many options for a TEM that was for path= ology usage verses one for research. Can anyone steer me in the right direc= tion. Of course cost has become a major issue also so we are trying to make= the best choice for our usage. Thanks James Vickroy BS, HT(A= SCP) Surgical and Autopsy Pathology Technical Supervisor Memorial= Medical Center 217-788-4046 ________________________________= This message (including any attachments) contains confidential informat= ion intended for a specific individual and purpose, and is protected by law= . If you are not the intended recipient, you should delete this message. An= y disclosure, copying, or distribution of this message, or the taking of an= y action based on it, is strictly prohibited. ------------------= ------------ _______________________________________________ Hist= onet mailing list Histonet@lists.utsouthwestern.edu http://lists.utso= uthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol= 90, Issue 29 **************************************** <= /mailto:mhale@carisls.com> From kenner.rita <@t> marshfieldclinic.org Tue May 24 13:52:20 2011 From: kenner.rita <@t> marshfieldclinic.org (Kenner, Rita) Date: Tue May 24 13:52:29 2011 Subject: [Histonet] Pathology Transmission Electron Micrscope&In-Reply-To= Message-ID: <201105241852.p4OIqNBU028998@mailhost2.mfldclin.edu> Hello Jim - We too are hoping to purchase a new TEM. I have collected quotes from JOEL, FEI/Phillips, and Hitachi, which range from $295,585(plus the cost of a camera) to $459,960. We did not seriously investigate Zeiss TEMs, as their new generation of scopes is geared towards nanotechnology, which is above and beyond our needs (and pocketbook!) Prices may have gone up somewhat since I last requested quotes - I will need to update when we get closer to capital request time. I also bear in mind that cost of service contracts, cost of consumable parts, and service histories of the various companies are all very important considerations when purchasing new equipment as well. Good luck to you! Rita Kenner B.S., HTL(ASCP) Electron Microscopy Lab Marshfield Clinic (715) 221-6182 ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Tue May 24 14:54:48 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 24 14:54:53 2011 Subject: [Histonet] WEt mount In-Reply-To: <6598.74839.qm@web125408.mail.ne1.yahoo.com> References: <6598.74839.qm@web125408.mail.ne1.yahoo.com> Message-ID: <915204.37303.qm@web65716.mail.ac4.yahoo.com> Instead of getting feedback that could be incomplete or even conflicting, why don't you consul a references book, like Bolles Lee vade-mecum? You will be better off. Ren? J. From: Candice Smoots To: Histonet Sent: Tuesday, May 24, 2011 1:04 PM Subject: [Histonet] WEt mount Hi guys. I?am new to this.. Can someone explain to me how to wet mount properly. What is it? ?I remain yours truely, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From d-emge <@t> northwestern.edu Tue May 24 14:57:01 2011 From: d-emge <@t> northwestern.edu (Donna J Emge) Date: Tue May 24 14:57:19 2011 Subject: [Histonet] Histology Technologist 2 Job Opening at Northwestern University, Chicago Campus Message-ID: <000601cc1a4c$bf577bc0$3e067340$@edu> Histology Technologist 2 Job ID 17253 Northwestern University Chicago Campus This position is for a small Core Laboratory that offers histology service University wide on both campuses and only works with mouse and other rodent tissue. Please review the complete job description requirements and apply online by following the information and link below. This is a great position where high quality and accuracy are valued above high speed. The histologists that work here are on top of their game with an enthusiasm to always make the lab better for all the researchers we serve. You can access the Northwestern University Careers site by going to: http://www.northwestern.edu/hr/jobs Click on the "Academic/Administrative Jobs" link. If you are an Internal Applicant click the Current Employee link. If you are an External Applicant click the External Applicant link. Once you are on the Careers Home page, please follow the below steps to view the Histology Technologist 2 position: 1. Click on the "Advanced Search" link located in the Basic Job Search box. 2. Type: Histology Technologist 2 into the Keywords text box. 3. Click on the Search button to view the search results. Thank you. From Farnana <@t> nehealth.com Tue May 24 13:33:17 2011 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Tue May 24 14:58:19 2011 Subject: [Histonet] Follicular staining of Melan A in lymph nodes Message-ID: <4DDBC1AD.26ED.00D9.1@nehealth.com> Good afternoon everyone, I am having a strange staining pattern that I have not seen before and I was hoping someone in histo land may be able to help. I am seeing Melan A staining in the follicles only in a sentinel node. There is no background and the staining is cytoplasmic. The node is actually negative for tumor and the staining is restricted to the follicles. I have looked online for any articles that may explain this but I haven't seen anything that would explain. thanks- Amy Farnan Histology Supervisor Northeast Health Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From sgoebel <@t> mirnarx.com Tue May 24 16:08:10 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Tue May 24 16:08:18 2011 Subject: [Histonet] Help me again please Message-ID: What dilution do you guys use on a rabbit polyclonal Ki-67 antibody...I just did 1/500 and got a crap ton of noise in the areas where the positive staining was. There are definite negative "rivers" flowing through the samples, but even the tonsil control has this same fuzziness to it. Any suggestions? Thanks PS-The antibody is Abcam Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From pamdefazio <@t> charter.net Tue May 24 17:33:55 2011 From: pamdefazio <@t> charter.net (Pam DeFazio) Date: Tue May 24 17:33:56 2011 Subject: [Histonet] PCNA stain Message-ID: <5C191FAC3F574A9A92F31B4B39D07B0D@OwnerPC> One of our pathologist has ask me to find somewhere to do this stain. I would like to send unstained slides for staining only, he will do the intreperation. Thanks for your help! Pam DeFazio, HT (ASCP) Athens Regional Medical Center Athens, GA From gguerzon <@t> live.com Tue May 24 23:12:37 2011 From: gguerzon <@t> live.com (Godfrey Guerzon) Date: Tue May 24 23:12:43 2011 Subject: [Histonet] IHC pos. & neg. control question In-Reply-To: <6EFF67C813C0488CB7156BC8FF67B14D@JoannePC> References: <6EFF67C813C0488CB7156BC8FF67B14D@JoannePC> Message-ID: This is my understanding about this CAP checklist item - CAP is looking for 2 types of negative controls: 1. Negative reagent control - this is satisfied by running a negative control slide from the same patient block through the whole process without the primary antibody. 2. Negative tissue control - this is satisfied by running a slide of multi tissue control containing tissues that are positive for the primary antibody and tissues that are negative for the primary antibody. The negative tissue on the multi tissue control will serve as the negative tissue control for the specific antibody. If you look at the positive control and the patient tissue only the areas that contain the target antigen will stain. There are other elements in the tissue that are known negative (e.g. smooth muscle in the small blood vessels will not stain with CD3 - then the smooth muscle in either the positive control and the patient tissue would serve as an internal negative tissue control for CD3). This negative result on the tissue elements that are expected not to stain with a specific antibody should be negative - hence - negative tissue control and should be noted in the documentation (preferably in the final report when commenting on the results of IHC stains). At Emory, we just had our CAP inspection (we only had a couple of deficiencies - xylene monitoring in the IHC area, Space and one corrected on site - labeling of chemicals without manufacturer's supplied expiration dates). Prior to inspection, we got clarification from CAP on this checklist item. CAP prefers the use of multi tissue control (containing both positive and negative tissue elements), however using negative tissue elements in the positive control and patient tissue are acceptable as negative tissue control for the specific antibody and it should be noted / documented accordingly. We document the negative control in our final reports when commenting on the results of IHC stains. This is my two cents contribution to the discussion. Godfrey > From: jshea121@roadrunner.com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 19 May 2011 21:11:01 -0400 > Subject: [Histonet] IHC pos. & neg. control question > > Curt is right, according to CAP ... > > ANP.22570 QC - Antibodies Phase II > > Appropriate negative controls are used. > > NOTE: Negative controls must assess the presence of nonspecific staining in patient tissue as well > > as the specificity of each antibody. Results of controls must be documented, either in internal > > laboratory records, or in the patient report. A statement in the report such as, "All controls show > > appropriate reactivity" is sufficient. > > A negative reagent control is used to assess nonspecific or aberrant staining in patient tissue related > > to the antigen retrieval conditions and/or detection system used. A separate section of patient tissue > > is processed using the same reagent and epitope retrieval protocol as the patient test slide, except > > that the primary antibody is omitted, and replaced by any one of the following: > > ? An unrelated antibody of the same isotype as the primary antibody (for monoclonal > > primary antibodies) > > ? An unrelated antibody from the same animal species as the primary antibody (for > > polyclonal primary antibodies) > > ? The negative control reagent included in the staining kit > > ? The diluent/buffer solution in which the primary antibody is diluted > > In general, a separate negative reagent control should be run for each block of patient tissue being > > immunostained; however, for cases in which there is simultaneous staining of multiple blocks from > > the same specimen with the same antibody (e.g. cytokeratin staining of multiple axillary sentinel > > lymph nodes), performing a single negative control on one of the blocks may be sufficient provided > > that all such blocks are fixed and processed identically. This exception does not apply to stains on > > different types of tissues or those using different antigen retrieval protocols or antibody detection > > systems. The laboratory director must determine which cases will have only one negative reagent > > control, and this must be specified in the department's procedure manual. > > The negative reagent control would ideally control for each reagent protocol and antibody retrieval > > condition; however, large antibody panels often employ multiple antigen retrieval procedures. In > > such cases, a reasonable minimum control would be to perform the negative reagent control using > > the most aggressive retrieval procedure in the particular antibody panel. Aggressiveness of antigen > > retrieval (in decreasing order) is as follows: pressure cooker; enzyme digestion, boiling; microwave; > > steamer; water bath. High pH retrieval should be considered more aggressive than comparable > > retrieval in citrate buffer at pH 6.0. > > It is also important to assess the specificity of each antibody by a negative tissue control, which > > must show no staining of tissues known to lack the antigen.The negative tissue control is processed > > using the same fixation, epitope retrieval and immunostaining protocols as the patient tissue. > > Unexpected positive staining of such tissues indicates that the test has lost specificity, perhaps > > because of improper antibody concentration or excessive antigen retrieval. Intrinsic properties of > > the test tissue may also be the cause of "non-specific" staining. For example, tissues with high > > endogenous biotin activity such as liver or renal tubules may simulate positive staining when using > > a detection method based on biotin labeling. > > A negative tissue control must be processed for each antibody in a given run. Any of the following > > can serve as a negative tissue control: > > 1. Multitissue blocks.These can provide simultaneous positive and negative tissue controls, > > and are considered "best practice" (see below). > > 2. The positive control slide or patient test slides, if these slides contain tissue elements > > that should not react with the antibody. > > 3. A separate negative tissue control slide. > > The type of negative tissue control used (i.e. separate sections, internal controls or multitissue > > blocks) should be specified in the laboratory manual (refer to ANP.22250). > > Multitissue blocks may be considered best practice and can have a major role in maintaining quality. > > When used as a combined positive and negative tissue control as mentioned above, they can serve > > as a permanent record documenting the sensitivity and specificity of every stain, particularly when > > mounted on the same slide as the patient tissue. When the components are chosen appropriately, > > multitissue blocks may be used for many different primary antibodies, decreasing the number of > > different control blocks needed by the laboratory. Multitissue blocks are also ideal for determining > > optimal titers of primary antibodies since they allow simultaneous evaluation of many different pieces > > of tissue. Finally, they are a useful and efficient means to screen new antibodies for sensitivity and > > specificity or new lots of antibody for consistency, which should be done before putting any antibody > > into diagnostic use. > > Evidence of Compliance: > > ? Written procedure for the use of negative reagent and tissue controls for IHC AND > > ? Patient reports or worksheet with control results > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmlongoria <@t> ecrmc.org Wed May 25 07:26:52 2011 From: dmlongoria <@t> ecrmc.org (Diana Martinez-Longoria) Date: Wed May 25 07:27:02 2011 Subject: [Histonet] bone marrow biopsy Message-ID: <12B71261212BE94BB9FB7735484B9FA101427A@EXMBX01.ecrmc.ci.el-centro.ca.us> Hello Histoland, I read on Histonet that some of you guys use EDTA for bone marrow core bx's. I was wondering what is the protocol? It will help us a lot. Thanks in advance! ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.   From kkmarshall <@t> anthc.org Wed May 25 08:20:37 2011 From: kkmarshall <@t> anthc.org (Marshall, Kimberly K) Date: Wed May 25 08:20:41 2011 Subject: [Histonet] Recycled Xylene Message-ID: Hello in Histo land. I know it is a subject brought up over and over again but I need to get the opinion of my fellow Histo techs on processing tissue with recycled Xylene. Yes I know it saves money and is better for the earth, but is the quality of the tissue the same??? Coverslipping and clearing slides with it I can see being ok, but processing with it??? It is not 100% after recycling. I could use any thought on the subject. Thanks in advance From lbustamante <@t> cvm.tamu.edu Wed May 25 08:51:44 2011 From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante) Date: Wed May 25 08:52:06 2011 Subject: [Histonet] Thank you Message-ID: <4DDCC320020000B900102982@CVM.TAMU.EDU> To all the Companies and Private labs that offered a microtome for sale. We have chosen one company to do business. I am very thankful for the great response we received. Sincerely. Lin Bustamante. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 From sfonner <@t> labpath.com Wed May 25 08:53:02 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Wed May 25 08:56:20 2011 Subject: [Histonet] RE: p63 In-Reply-To: References: <0494A7D4E8CC254EA2FB81464982E3786DCA72@S10MAILD001N1.SH10.lan> Message-ID: <000001cc1ae3$10ac7de0$320579a0$@com> We get ours from Ventana. Sheila Fonner, HT (ASCP) KDL Pathology Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Monday, May 23, 2011 1:46 PM To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: p63 I believe the only place you can buy p63 is through Biocare, www.biocare.net. Lynne Bell, HT (ASCP) Histology Team Leader 802-371-4923 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Wed May 25 09:07:33 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed May 25 09:07:40 2011 Subject: [Histonet] Recycled Xylene In-Reply-To: References: Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2ACEB@EXCHANGE3.huntingtonhospital.com> We process with recycled xylene and have no problems. We also use it for staining but use fresh for coverslipping. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Wednesday, May 25, 2011 6:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled Xylene Hello in Histo land. I know it is a subject brought up over and over again but I need to get the opinion of my fellow Histo techs on processing tissue with recycled Xylene. Yes I know it saves money and is better for the earth, but is the quality of the tissue the same??? Coverslipping and clearing slides with it I can see being ok, but processing with it??? It is not 100% after recycling. I could use any thought on the subject. Thanks in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague <@t> pathologyarts.com Wed May 25 09:18:04 2011 From: c.tague <@t> pathologyarts.com (Curt Tague) Date: Wed May 25 09:20:35 2011 Subject: [Histonet] Recycled Xylene In-Reply-To: References: Message-ID: <02b201cc1ae6$8fd80140$af8803c0$@tague@pathologyarts.com> We recycle it and have had no problems with processing, no complaints from the docs. Curt -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Wednesday, May 25, 2011 6:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled Xylene Hello in Histo land. I know it is a subject brought up over and over again but I need to get the opinion of my fellow Histo techs on processing tissue with recycled Xylene. Yes I know it saves money and is better for the earth, but is the quality of the tissue the same??? Coverslipping and clearing slides with it I can see being ok, but processing with it??? It is not 100% after recycling. I could use any thought on the subject. Thanks in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 25 10:11:22 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 25 10:11:27 2011 Subject: [Histonet] Recycled Xylene In-Reply-To: References: Message-ID: <295111.4215.qm@web65713.mail.ac4.yahoo.com> If you are using a good "cracking" recycling instrument the recycled xylene = 100% xylene and there cannot be any differences in behavior against "pure-unused-mew" xylene. That is what I always found for more than 15 years. Ren? J. From: "Marshall, Kimberly K" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 25, 2011 9:20 AM Subject: [Histonet] Recycled Xylene Hello in Histo land. ? I know it is a subject brought up over and over again but I need to get the opinion of my fellow Histo techs on processing tissue with recycled Xylene.? Yes I know it saves money and is better for the earth, but is the quality of the tissue the same??? Coverslipping and clearing slides with it I can see being ok, but processing with it??? It is not 100% after recycling.? I could use any thought on the subject.? Thanks in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague <@t> pathologyarts.com Wed May 25 10:11:30 2011 From: c.tague <@t> pathologyarts.com (Curt Tague) Date: Wed May 25 10:11:36 2011 Subject: [Histonet] pathology software Message-ID: <02b801cc1aee$06bbeea0$1433cbe0$@tague@pathologyarts.com> We are considering a change in software LIS. We are a typical small private path lab, currently path and cyto only but are looking at adding clinical in the future. Need to have the EMR update capability, I think it's an HL7 interface, to transfer results electronically and upload directly to a clients EMR. What are you all using in your labs? I can't go with some of the big guys like Cerner, though it is nice and top of the line, it's a little out of my budget. I need to have the ability to have several different label formats, some of our hospital clients like labels with little info, some like more info. So I want to be able to modify a slide label based on each clients desire and then save it to the database as specific for that dr. Anyone out there have a product that you're just over the top impressed with, produce and customer service combined? Thanks, Curt From d-emge <@t> northwestern.edu Wed May 25 10:15:19 2011 From: d-emge <@t> northwestern.edu (Donna J Emge) Date: Wed May 25 10:15:37 2011 Subject: [Histonet] Histology Technologist 2 Job Opening at Northwestern University, Chicago Campus Message-ID: <004801cc1aee$8f86b350$ae9419f0$@edu> Histology Technologist 2 Job ID 17253 Northwestern University Chicago Campus This position is for a small Core Laboratory that offers histology service University wide on both campuses and only works with mouse and other rodent tissue. Please review the complete job description requirements and apply online by following the information and link below. This is a great position where high quality and accuracy are valued above high speed. The histologists that work here are on top of their game with an enthusiasm to always make the lab better for all the researchers we serve. You can access the Northwestern University Careers site by going to: http://www.northwestern.edu/hr/jobs Click on the "Academic/Administrative Jobs" link. If you are an Internal Applicant click the Current Employee link. If you are an External Applicant click the External Applicant link. Once you are on the Careers Home page, please follow the below steps to view the Histology Technologist 2 position: 1. Click on the "Advanced Search" link located in the Basic Job Search box. 2. Type: Histology Technologist 2 into the Keywords text box. 3. Click on the Search button to view the search results. Thank you. From ChaseM <@t> childrensdayton.org Wed May 25 10:32:23 2011 From: ChaseM <@t> childrensdayton.org (Matthew Chase) Date: Wed May 25 10:37:21 2011 Subject: [Histonet] Dayton Ohio Histotech Needed Message-ID: <9719621676F71F49AEA7CCC6F4561B3D045A995446@PEXCHNG1.cmc-dayton.org> Hey All A fulltime Histotech position is open at Dayton Children's Hospital in Dayton Ohio. We are a small hospital. We have one part time, one full time (that could be you) and myself. We process about 5000 cases a year, we average about 15-40 blocks a day. This is Dayton Children's Hospital, good benefits, not a whole lot of stress. If you're looking for a great place with a great group of people give me a call, or call HR at 937-641-8090 and ask for Dan Krauss. Or just apply online at http://www.childrensdayton.org/cms/careers/index.html If you want more specifics you can call me at 641-3000 ext 8229. Please no Headhunters, we are not allowed to use employment agencies, thanks. Matt Chase Supervisor of Pathology ________________________________ NOTICE: The information contained in this e-mail and any accompanying documents or files is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited and possibly a violation of federal/state law or regulations. If you received this information in error, please notify The Children's Medical Center of Dayton immediately via telephone at (937) 641-5293, or via electronic mail cmcconfidentiality@childrensdayton.org and promptly destroy the original message. Thank you. From campbellj <@t> muhlbauerlab.com Wed May 25 10:38:09 2011 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Wed May 25 10:38:14 2011 Subject: [Histonet] Recycled Xylene In-Reply-To: <295111.4215.qm@web65713.mail.ac4.yahoo.com> References: <295111.4215.qm@web65713.mail.ac4.yahoo.com> Message-ID: Very true because if you notice the label on a purchased bottle of xylene it says "Xylenes". Your recycled product should be pure xylene and thus a higher purity than what you started with. Our lab has been recycling since the mid '90's. We no longer process with xylene but we still have it in the lab for various things. Hope this helps. On Wed, May 25, 2011 at 11:11 AM, Rene J Buesa wrote: > If you are using a good "cracking" recycling instrument the recycled xylene > = 100% xylene and there cannot be any differences in behavior against > "pure-unused-mew" xylene. That is what I always found for more than 15 > years. > Ren? J. > > From: "Marshall, Kimberly K" > To: histonet@lists.utsouthwestern.edu > Sent: Wednesday, May 25, 2011 9:20 AM > Subject: [Histonet] Recycled Xylene > > Hello in Histo land. > > I know it is a subject brought up over and over again but I need to > get the opinion of my fellow Histo techs on processing tissue with > recycled Xylene. Yes I know it saves money and is better for the earth, > but is the quality of the tissue the same??? Coverslipping and clearing > slides with it I can see being ok, but processing with it??? It is not > 100% after recycling. I could use any thought on the subject. > > Thanks in advance > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 From rjbuesa <@t> yahoo.com Wed May 25 11:05:15 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 25 11:05:19 2011 Subject: [Histonet] Recycled Xylene In-Reply-To: References: <295111.4215.qm@web65713.mail.ac4.yahoo.com> Message-ID: <660593.25408.qm@web65710.mail.ac4.yahoo.com> The word "xylenes" in a "pure" xylene bottle means?it contains?a mixture of ORTHO-; ?META-; ?and PARA-xylene (3 different xylene molecular configurations), hence the title "xylenes". After you distill your used xylene, you will probably?end with a very similar proportion of the 3 molecules and it will be "xylenes" also. Ren? J. From: Jennifer Campbell To: Rene J Buesa Cc: "Marshall, Kimberly K" ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, May 25, 2011 11:38 AM Subject: Re: [Histonet] Recycled Xylene Very true because if you notice the label on a purchased bottle of xylene it says "Xylenes". Your recycled product should be pure xylene and thus a higher purity than what you started with. Our lab has been recycling since the mid '90's. We no longer process with xylene but we still have it in the lab for various things. Hope this helps. On Wed, May 25, 2011 at 11:11 AM, Rene J Buesa wrote: If you are using a good "cracking" recycling instrument the recycled xylene = 100% xylene and there cannot be any differences in behavior against "pure-unused-mew" xylene. That is what I always found for more than 15 years. >Ren? J. > >From: "Marshall, Kimberly K" >To: histonet@lists.utsouthwestern.edu >Sent: Wednesday, May 25, 2011 9:20 AM >Subject: [Histonet] Recycled Xylene > >Hello in Histo land. > >? I know it is a subject brought up over and over again but I need to >get the opinion of my fellow Histo techs on processing tissue with >recycled Xylene.? Yes I know it saves money and is better for the earth, >but is the quality of the tissue the same??? Coverslipping and clearing >slides with it I can see being ok, but processing with it??? It is not >100% after recycling.? I could use any thought on the subject.? > >Thanks in advance >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 From d-emge <@t> northwestern.edu Wed May 25 11:50:01 2011 From: d-emge <@t> northwestern.edu (Donna J Emge) Date: Wed May 25 11:50:18 2011 Subject: [Histonet] Re: Histology Technologist 2 Job Opening at Northwestern University, Chicago Campus Message-ID: <001501cc1afb$c9ce3670$5d6aa350$@edu> Hi Fellow Histonetters, The ad I posted is not from a job placement agency. Please follow the Northwestern University HR link and navigation instructions I posted in the original ad to fill out the job application and send your resume. Thanks, Donna Donna J. Emge, ASCP-HT Mouse Histology and Phenotyping Laboratory Manager Northwestern University Olson Pavilion 8-333 710 North Fairbanks Court Chicago, IL 60611 d-emge@northwestern.edu 312-503-2679 From JWeems <@t> sjha.org Wed May 25 12:17:47 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed May 25 12:17:52 2011 Subject: [Histonet] HSV control Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640820E10477@CHEXCMS10.one.ads.che.org> Our NSH control banker is searching for me, but does anyone anywhere have any HSV control? Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From tpodawiltz <@t> lrgh.org Wed May 25 12:34:22 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed May 25 12:34:28 2011 Subject: [Histonet] Unlabeled specimens Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DEBE7C40@LRGHEXVS1.practice.lrgh.org> What are your procedures for rejection and correction when you receive either a surgical or Nongyn specimen that has incomplete information on the requisitions or the container is not labeled properly? Do you send it back to the provider's office? Have them come to your lab to make the corrections? Throw away the specimen? Tom THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From rjbuesa <@t> yahoo.com Wed May 25 14:59:46 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 25 14:59:51 2011 Subject: [Histonet] Unlabeled specimens In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DEBE7C40@LRGHEXVS1.practice.lrgh.org> References: <38667E7FB77ECD4E91BFAEB8D986386323DEBE7C40@LRGHEXVS1.practice.lrgh.org> Message-ID: <604751.95969.qm@web65705.mail.ac4.yahoo.com> I used to send it back. Ren? J. From: "Podawiltz, Thomas" To: "'Histonet@lists.utsouthwestern.edu'" Sent: Wednesday, May 25, 2011 1:34 PM Subject: [Histonet] Unlabeled specimens What are your procedures for rejection and correction when you receive either a surgical or Nongyn specimen that? has incomplete information on the requisitions or the container is not labeled properly? Do you send it back to the provider's office? Have them come to your lab to make the corrections? Throw away the specimen? Tom THIS MESSAGE IS CONFIDENTIAL.? This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.? If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hlukey <@t> msn.com Wed May 25 15:33:40 2011 From: hlukey <@t> msn.com (Hugh Luk) Date: Wed May 25 15:33:44 2011 Subject: [Histonet] Unlabeled specimens In-Reply-To: <604751.95969.qm@web65705.mail.ac4.yahoo.com> References: <38667E7FB77ECD4E91BFAEB8D986386323DEBE7C40@LRGHEXVS1.practice.lrgh.org>, <604751.95969.qm@web65705.mail.ac4.yahoo.com> Message-ID: Hi Tom, Consider how long it would take to send it back to get a response. Some clients may be far away. If that Non-gyn needs to be processed, we would call them, document the information and contacts, and politely remind them to fill out the forms. Yes, this can be time consuming. In these days of barcoding and specimen tracking, nothing is thrown out, and the majority of rejected specimens get sent back to the physician/group. Sometimes they don't come back, but that's their prerogative. Hugh-Hawaii > From: "Podawiltz, Thomas" > To: "'Histonet@lists.utsouthwestern.edu'" > Sent: Wednesday, May 25, 2011 1:34 PM > Subject: [Histonet] Unlabeled specimens > > What are your procedures for rejection and correction when you receive either a surgical or Nongyn specimen that has incomplete information on the requisitions or the container is not labeled properly? > Do you send it back to the provider's office? > Have them come to your lab to make the corrections? > Throw away the specimen? > > Tom > > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dw18 <@t> uchicago.edu Wed May 25 15:46:39 2011 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Wed May 25 15:46:43 2011 Subject: [Histonet] Re: DAB reduction In-Reply-To: <201105221704.DCV16718@mx01.uchicago.edu> References: <201105221704.DCV16718@mx01.uchicago.edu> Message-ID: <20110525154639.AQB77321@mstore03.uchicago.edu> Hello Naira & Histonet I'm a bit behind on this thread but you might still be interested in this idea. There is one way you might be able to minimize your DAB signal, but only if it is intensified with a metal salt such as cobalt and/or nickel. You can (slowly) chelate out the metal ions with EDTA. It will still leave the DAB undissolved but now as a golden brown deposit instead of e.g. blue-black. If you determined your end point for the staining reaction with Ni/Co rather than with pure DAB, the residual DAB will be much less intense than you would aim for with pure DAB. I discovered this by accident, using 0.1% of EDTA dihydrate in buffer, and it took a couple of days to extract at RT. You could try using more or, if your antigen retrieval solution includes EDTA, you could try repeating that (with the heating to speed things up) until the DAB just looks brown. Since the DAB will still be there, I agree with Amos Brooks that you should re-react with a different chromogen. It is not necessary to switch enzymes, however: peroxidase will still work. The DAB precipitate completely coats the original Ag-Ab-peroxidase, blocking further reaction with the new chromogen (and, if you do more Ag retrieval, you would remove accessible complexes that way). I do sequential peroxidase reactions routinely, with no x-rxn after DAB 1st round. I hope you were not planning to re-stain for the same antigen; it will be masked by the DAB ppt. -David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery ORIGINAL MESSAGES Re: Histonet Digest, Vol 90, Issue 22 Message: 2 Date: Thu, 19 May 2011 11:55:14 -0500 From: Margaryan, Naira Subject: [Histonet] FW: How to remove DAB to restain with DAB Hello, I would like to repeat my DAB staining on the same slide with DAB I run before. I know that "acid alcohol" will remove hematoxylin but how to remove DAB? I appreciate to any suggestion. Thanks in advance, Naira ==================== Re: Histonet Digest, Vol 90, Issue 23 Message: 9 Date: Thu, 19 May 2011 11:23:54 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] FW: How to remove DAB to restain with DAB DAB reaction is almost permanent and extremely difficult to destain. Ren? J. ==================== Re: Histonet Digest, Vol 90, Issue 23 Message: 18 Date: Thu, 19 May 2011 12:26:32 -0700 (PDT) From: Keith Mikoff Subject: [Histonet] Re: Histonet Digest, Vol 90, Issue 22 2. FW: How to remove DAB to restain with DAB (Margaryan, Naira) Hi Naira, The short answer is no, it can't be done. Unless there is a way to release the antibody from the binding site without damaging the binding site, it can't be done. That said, somehow it should be possible to find a reproduce-able fix for this kind of issue, by either mending the binding site, or enhancing the activation of the already applied antibody complex and/or chromogen. Some really cool tricks if practical or possible. It seems like it should, maybe with the application of an mild acidic or basic wash... with just the right reagent, the right amount, right application and at the right pH. Something to think about. Keith M. Mikoff, HTL (ASCP) ==================== Re: Histonet Digest, Vol 90, Issue 26 Message: 10 Date: Fri, 20 May 2011 16:25:27 -0400 From: Amos Brooks Subject: [Histonet] FW: How to remove DAB to restain with DAB Hi, There is not any practical way to remove the DAB. I think I read about boiling it in a salt (of some sort) solution removing the DAB. Honestly I never bothered trying it as you need to ask yourself "What is this doing to my target antigen?". You should consider just restaining it with the DAB in place using either Alkaline Phosphatase and Fast Red or using a fluorescent detection if the DAB is interfering with the signal. Amos ================== Re: Histonet Digest, Vol 90, Issue 26 Message: 14 Date: Sat, 21 May 2011 09:31:51 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] FW: How to remove DAB to restain with DAB I have removed DAB by using the probe cleaning reagents from the Dako Autostainer but Amos is right this destroys the antigen site so it is of no use. If you really only have that one section I guess you might be able to use it as an H&E. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech ================== From garret.t.miyamoto <@t> us.army.mil Wed May 25 16:27:29 2011 From: garret.t.miyamoto <@t> us.army.mil (Miyamoto, Garret T Mr CIV USA USAMEDCOM) Date: Wed May 25 16:27:35 2011 Subject: [Histonet] Re: Recycled Xylene In-Reply-To: <0LLR00GTEGQKKP00@mail23.us.army.mil> References: <0LLR00GTEGQKKP00@mail23.us.army.mil> Message-ID: Kimberly, We have been using recycled xylene for our tissue processing for years. No problems at all with the tissues. Garret Miyamoto Tripler Army Medical Center ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Wednesday, May 25, 2011 7:03 am Subject: Histonet Digest, Vol 90, Issue 31 To: histonet@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Recycled Xylene (Curt Tague) > 2. Re: Recycled Xylene (Rene J Buesa) > 3. pathology software (Curt Tague) > 4. Histology Technologist 2 Job Opening at Northwestern > University, Chicago Campus (Donna J Emge) > 5. Dayton Ohio Histotech Needed (Matthew Chase) > 6. Re: Recycled Xylene (Jennifer Campbell) > 7. Re: Recycled Xylene (Rene J Buesa) > 8. Re: Histology Technologist 2 Job Opening at Northwestern > University, Chicago Campus (Donna J Emge) > > > ------------------------------------------------------------------- > --- > > Message: 1 > Date: Wed, 25 May 2011 07:18:04 -0700 > From: "Curt Tague" < > Subject: RE: [Histonet] Recycled Xylene > To: "'Marshall, Kimberly K'" <, > < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > We recycle it and have had no problems with processing, no complaints from > the docs. > > Curt > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, > Kimberly K > Sent: Wednesday, May 25, 2011 6:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Recycled Xylene > > Hello in Histo land. > > I know it is a subject brought up over and over again but I need to > get the opinion of my fellow Histo techs on processing tissue with > recycled Xylene. Yes I know it saves money and is better for the earth, > but is the quality of the tissue the same??? Coverslipping and clearing > slides with it I can see being ok, but processing with it??? It is not > 100% after recycling. I could use any thought on the subject. > > Thanks in advance > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 2 > Date: Wed, 25 May 2011 08:11:22 -0700 (PDT) > From: Rene J Buesa < > Subject: Re: [Histonet] Recycled Xylene > To: "Marshall, Kimberly K" <, > "histonet@lists.utsouthwestern.edu" > < > Message-ID: < > Content-Type: text/plain; charset=iso-8859-1 > > If you are using a good "cracking" recycling instrument the recycled xylene = 100% xylene and there cannot be any differences in behavior against "pure-unused-mew" xylene. That is what I always found for more than 15 years. > Ren? J. > > From: "Marshall, Kimberly K" < > To: histonet@lists.utsouthwestern.edu > Sent: Wednesday, May 25, 2011 9:20 AM > Subject: [Histonet] Recycled Xylene > > Hello in Histo land. > > ? I know it is a subject brought up over and over again but I need to > get the opinion of my fellow Histo techs on processing tissue with > recycled Xylene.? Yes I know it saves money and is better for the earth, > but is the quality of the tissue the same??? Coverslipping and clearing > slides with it I can see being ok, but processing with it??? It is not > 100% after recycling.? I could use any thought on the subject.? > > Thanks in advance > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 3 > Date: Wed, 25 May 2011 08:11:30 -0700 > From: "Curt Tague" < > Subject: [Histonet] pathology software > To: < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > We are considering a change in software LIS. We are a typical small private > path lab, currently path and cyto only but are looking at adding clinical in > the future. Need to have the EMR update capability, I think it's an HL7 > interface, to transfer results electronically and upload directly to a > clients EMR. > > What are you all using in your labs? I can't go with some of the big guys > like Cerner, though it is nice and top of the line, it's a little out of my > budget. I need to have the ability to have several different label formats, > some of our hospital clients like labels with little info, some like more > info. So I want to be able to modify a slide label based on each clients > desire and then save it to the database as specific for that dr. > > Anyone out there have a product that you're just over the top impressed > with, produce and customer service combined? > > > > Thanks, > > Curt > > > > > > ------------------------------ > > Message: 4 > Date: Wed, 25 May 2011 10:15:19 -0500 > From: "Donna J Emge" < > Subject: [Histonet] Histology Technologist 2 Job Opening at > Northwestern University, Chicago Campus > To: < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Histology Technologist 2 > Job ID 17253 > Northwestern University > Chicago Campus > > > > This position is for a small Core Laboratory that offers histology service > University wide on both campuses and only works with mouse and other rodent > tissue. Please review the complete job description requirements and apply > online by following the information and link below. This is a great position > where high quality and accuracy are valued above high speed. The > histologists that work here are on top of their game with an enthusiasm to > always make the lab better for all the researchers we serve. > > > You can access the Northwestern University Careers site by going to: > http://www.northwestern.edu/hr/jobs > > > Click on the "Academic/Administrative Jobs" link. > > If you are an Internal Applicant click the Current Employee link. > If you are an External Applicant click the External Applicant link. > > Once you are on the Careers Home page, please follow the below steps to view > the Histology Technologist 2 position: > 1. Click on the "Advanced Search" link located in the Basic Job Search box. > 2. Type: Histology Technologist 2 into the Keywords text box. > 3. Click on the Search button to view the search results. > > Thank you. > > > > > > ------------------------------ > > Message: 5 > Date: Wed, 25 May 2011 11:32:23 -0400 > From: "Matthew Chase" < > Subject: [Histonet] Dayton Ohio Histotech Needed > To: "'Histonet@lists.utsouthwestern.edu'" > < > Message-ID: > < > Content-Type: text/plain; charset="us-ascii" > > Hey All > > A fulltime Histotech position is open at Dayton Children's Hospital in Dayton Ohio. We are a small hospital. We have one part time, one full time (that could be you) and myself. We process about 5000 cases a year, we average about 15-40 blocks a day. This is Dayton Children's Hospital, good benefits, not a whole lot of stress. If you're looking for a great place with a great group of people give me a call, or call HR at 937-641-8090 and ask for Dan Krauss. Or just apply online at http://www.childrensdayton.org/cms/careers/index.html > > > > If you want more specifics you can call me at 641-3000 ext 8229. > > > > Please no Headhunters, we are not allowed to use employment agencies, thanks. > > > > Matt Chase > > Supervisor of Pathology > > > ________________________________ > NOTICE: The information contained in this e-mail and any accompanying documents or files is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited and possibly a violation of federal/state law or regulations. > > If you received this information in error, please notify The Children's Medical Center of Dayton immediately via telephone at (937) 641-5293, or via electronic mail cmcconfidentiality@childrensdayton.org and promptly destroy the original message. Thank you. > > > ------------------------------ > > Message: 6 > Date: Wed, 25 May 2011 11:38:09 -0400 > From: Jennifer Campbell < > Subject: Re: [Histonet] Recycled Xylene > To: Rene J Buesa < > Cc: "histonet@lists.utsouthwestern.edu" > < > Message-ID: < > Content-Type: text/plain; charset=ISO-8859-1 > > Very true because if you notice the label on a purchased bottle of xylene it > says "Xylenes". Your recycled product should be pure xylene and thus a > higher purity than what you started with. > > Our lab has been recycling since the mid '90's. We no longer process with > xylene but we still have it in the lab for various things. > > Hope this helps. > > On Wed, May 25, 2011 at 11:11 AM, Rene J Buesa < wrote: > > > If you are using a good "cracking" recycling instrument the recycled xylene > > = 100% xylene and there cannot be any differences in behavior against > > "pure-unused-mew" xylene. That is what I always found for more than 15 > > years. > > Ren? J. > > > > From: "Marshall, Kimberly K" < > > To: histonet@lists.utsouthwestern.edu > > Sent: Wednesday, May 25, 2011 9:20 AM > > Subject: [Histonet] Recycled Xylene > > > > Hello in Histo land. > > > > I know it is a subject brought up over and over again but I need to > > get the opinion of my fellow Histo techs on processing tissue with > > recycled Xylene. Yes I know it saves money and is better for the earth, > > but is the quality of the tissue the same??? Coverslipping and clearing > > slides with it I can see being ok, but processing with it??? It is not > > 100% after recycling. I could use any thought on the subject. > > > > Thanks in advance > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Jen Campbell, HT(ASCP) > Supervisor of Technical Services > Muhlbauer Dermatopathology Laboratory > 61 Monroe Avenue, Ste B > Pittsford NY 14534 > P: 585.586.5166 > F: 585.586.3137 > > > ------------------------------ > > Message: 7 > Date: Wed, 25 May 2011 09:05:15 -0700 (PDT) > From: Rene J Buesa < > Subject: Re: [Histonet] Recycled Xylene > To: Jennifer Campbell < > Cc: "histonet@lists.utsouthwestern.edu" > < > Message-ID: < > Content-Type: text/plain; charset=iso-8859-1 > > The word "xylenes" in a "pure" xylene bottle means?it contains?a mixture of ORTHO-; ?META-; ?and PARA-xylene (3 different xylene molecular configurations), hence the title "xylenes". > After you distill your used xylene, you will probably?end with a very similar proportion of the 3 molecules and it will be "xylenes" also. > Ren? J. > > From: Jennifer Campbell < > To: Rene J Buesa < > Cc: "Marshall, Kimberly K" <; "histonet@lists.utsouthwestern.edu" < > Sent: Wednesday, May 25, 2011 11:38 AM > Subject: Re: [Histonet] Recycled Xylene > > > Very true because if you notice the label on a purchased bottle of xylene it says "Xylenes". Your recycled product should be pure xylene and thus a higher purity than what you started with. > > Our lab has been recycling since the mid '90's. We no longer process with xylene but we still have it in the lab for various things. > > Hope this helps. > > > On Wed, May 25, 2011 at 11:11 AM, Rene J Buesa < wrote: > > If you are using a good "cracking" recycling instrument the recycled xylene = 100% xylene and there cannot be any differences in behavior against "pure-unused-mew" xylene. That is what I always found for more than 15 years. > >Ren? J. > > > >From: "Marshall, Kimberly K" < > >To: histonet@lists.utsouthwestern.edu > >Sent: Wednesday, May 25, 2011 9:20 AM > >Subject: [Histonet] Recycled Xylene > > > >Hello in Histo land. > > > >? I know it is a subject brought up over and over again but I need to > >get the opinion of my fellow Histo techs on processing tissue with > >recycled Xylene.? Yes I know it saves money and is better for the earth, > >but is the quality of the tissue the same??? Coverslipping and clearing > >slides with it I can see being ok, but processing with it??? It is not > >100% after recycling.? I could use any thought on the subject.? > > > >Thanks in advance > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > -- > Jen Campbell, HT(ASCP) > Supervisor of Technical Services > Muhlbauer Dermatopathology Laboratory > 61 Monroe Avenue, Ste B > Pittsford NY 14534 > P: 585.586.5166 > F: 585.586.3137 > > ------------------------------ > > Message: 8 > Date: Wed, 25 May 2011 11:50:01 -0500 > From: "Donna J Emge" < > Subject: [Histonet] Re: Histology Technologist 2 Job Opening at > Northwestern University, Chicago Campus > To: < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Hi Fellow Histonetters, > > > > The ad I posted is not from a job placement agency. Please follow the > Northwestern University HR link and navigation instructions I posted in the > original ad to fill out the job application and send your resume. > > > > Thanks, > > Donna > > > > > > Donna J. Emge, ASCP-HT > Mouse Histology and Phenotyping Laboratory Manager > Northwestern University > Olson Pavilion 8-333 > 710 North Fairbanks Court > Chicago, IL 60611 > d-emge@northwestern.edu > 312-503-2679 > > > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 90, Issue 31 > **************************************** From Diane.Tokugawa <@t> kp.org Wed May 25 18:02:47 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Wed May 25 18:03:15 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 05/24/2011 and will not return until 05/31/2011. Note: For Cytology issues, please call Molly (day) at 8-421-5487 or Eric (eve) at 8-421-5405, For Histology issues, please call the general histology lab 8-421- 5408, Mario 8-421-4961 (day), Kiran 8-421-5404 (late afternoon/eve) or Wanda Lau for Cyto/Histo issues 8-421-5426. From amosbrooks <@t> gmail.com Wed May 25 20:42:38 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed May 25 20:42:44 2011 Subject: [Histonet] bone marrow biopsy Message-ID: Hi, I have pasted the procedure below. Please be aware that this comes with the caveat that it is a very slow process compared to the strong acid solutions that we are most accustomed to. If there is a diagnostic need to expedite the results, then this may not be the best option. Incidentally just so no one thinks I'm wise enough to come up with this on my own, here is the reference (with original references at the end of that link). http://www.ihcworld.com/_protocols/histology/decalcification_solution.htm I am wise enough to give credit where it is due :-) Good luck, Amos 10% EDTA (pH7.4), or 10% EDTA/TRIS-HCl (pH 7.4), or 10% EDTA with 0.07% (w/v) Glycerol (pH 7.4) * * EDTA is a chelating agent, and it can be made 10% solution with distilled water, pH 7.4. This is also the preferred solution for decalcifying bone material for transmission electron microscopy. Specimens can be decalcified in this solution over several days up to several weeks in a refrigerator at 4 C, depending on degree of mineralization and size of specimen. The fresh solution is changed several times or once a week. After decalcification, samples can be routinely processed and embedded in paraffin. Several studies have shown that EDTA decalcified bone material preserves DNA better, and preferable for ISH analysis, and TUNEL staining. It is also suitable for most of immunohistochemical staining protocols. Message: 12 Date: Wed, 25 May 2011 12:26:52 +0000 From: Diana Martinez-Longoria Subject: [Histonet] bone marrow biopsy To: "Histonet@lists.utsouthwestern.edu" Message-ID: < 12B71261212BE94BB9FB7735484B9FA101427A@EXMBX01.ecrmc.ci.el-centro.ca.us> Content-Type: text/plain; charset="us-ascii" Hello Histoland, I read on Histonet that some of you guys use EDTA for bone marrow core bx's. I was wondering what is the protocol? It will help us a lot. Thanks in advance! From rfisher0126 <@t> msn.com Wed May 25 21:03:40 2011 From: rfisher0126 <@t> msn.com (Renee Fisher) Date: Wed May 25 21:03:49 2011 Subject: [Histonet] validating the Leica Peloris Message-ID: Our lab is in the process of validating the Leica Peloris II tissue processor, is there anyone out there who has the Peloris II? How did you validate? how many runs, and what type of tissue did you use? Thank you for your in put. From arme <@t> optonline.net Wed May 25 23:50:43 2011 From: arme <@t> optonline.net (American ReSource Medical) Date: Wed May 25 23:50:47 2011 Subject: [Histonet] Available for sale Message-ID: (1) Mopec MB100 Grossing Station http://media2.mopec.com/media/pdf/MBSeriesGrossingStationBrochure.pdf excellent condition Mark Sofferman, President American ReSource Medical 324 West Englewood Avenue Teaneck, NJ 07666 P: 201.833.1550 F: 201.833.1575 From Reuel.Cornelia <@t> tsrh.org Thu May 26 11:03:49 2011 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Thu May 26 11:04:32 2011 Subject: [Histonet] Histotech needed in dallas texas Message-ID: <4DDE3395.077E.00C5.1@tsrh.org> Research - Histotechnologist This position is responsible for supporting research projects in histological techniques, immunohistochemistry, hard tissue histology and image analysis of pathological specimens with Molecular biology, confocal microscopy and laser capture microdissection background. B.S. in medical technology/histology or B.S. in biomedical sciences required. HT (ASCP) or eligible preferred, with a minimum of five years work experience. M-F, 8 a.m. - 4:30 p.m. For more information or to apply on-line, please visit us at www.tsrhc.org or contact Shonna Norman at tsrhhr@tsrh.org. Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, Texas 75219 214-559-7590 Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From relia1 <@t> earthlink.net Thu May 26 11:06:51 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu May 26 11:06:59 2011 Subject: [Histonet] Exciting Opportunity with a Brand New Lab in Kansas City Histotech needed Mohs a plus or learn Mohs here!! Message-ID: <683666A688464BA9AAA8F00AE6F8A30C@ownerf1abaad51> Hi Histonetters!! How are you? I have a new histology position and I need your help. I am currently working with a brand new laboratory in the Kansas City area on an exciting opportunity for an ASCP certified or eligible histotech with a brand new lab in Kansas City, KS. This is a dayshift full time M-F permanent position. My client offers excellent compensation, benefits and the opportunity to learn Mohs. Experienced Mohs Techs, Histotechs who want to learn Mohs and New Grads are welcome to apply. My question is do you know of anyone who might be interested in this position? I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 500.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From coralmani <@t> yahoo.co.in Thu May 26 11:30:27 2011 From: coralmani <@t> yahoo.co.in (mani kandan) Date: Thu May 26 11:30:31 2011 Subject: [Histonet] mouse femour section Message-ID: <649752.95380.qm@web94710.mail.in2.yahoo.com> hai, ???? i am working with mouse femours, i found some cells in the section looks like a ring shaped cells i want to know what r they,can u help me in this regard. M.Manikandan, Researcher, Stemcell unit, King Saud university, Riyadh,KSA +966552012697 From mfisher <@t> ecrmc.org Thu May 26 12:22:41 2011 From: mfisher <@t> ecrmc.org (Marcia Fisher) Date: Thu May 26 12:22:47 2011 Subject: [Histonet] Unlabeled Specimens Message-ID: If the specimen is in-house, we walk it back to the department for correction. If outside the hospital, the courier returns it to the clinic/doctor's office for correction. All mislabeled specimens, however, have a form filled out (Specimen Rejection form), "copies" of the specimen container are made and a report made to QRM is severe enough to warrant. This is all in accordance with CAP. Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.   From algranth <@t> email.arizona.edu Thu May 26 14:28:38 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu May 26 14:29:04 2011 Subject: [Histonet] off topic/on topic Message-ID: <41DD03DE-C258-4FC8-94A9-CD24210147DE@email.arizona.edu> This is mostly for the girls - I dropped into the Ulta store yesterday to pick up a few things and the sales person wanted me to try this somewhat new product by Benefit. It was a lip and cheek tint - came in a little bottle - looked like mucicarmine working solution. I looked at the box that it came in and sure enough, one of the first ingredients was CARMINE! So I had to relate a few of the stories about beetles and Carmine and pirates that I learned from reading "A Perfect Red" recommended by Dick Dapson...wonder if I bored her? Maybe she won't bother me the next time I'm rushing through the store trying to get my stuff and get out of there. Andi From rjbuesa <@t> yahoo.com Thu May 26 14:58:53 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 26 14:58:56 2011 Subject: [Histonet] mouse femour section In-Reply-To: <649752.95380.qm@web94710.mail.in2.yahoo.com> References: <649752.95380.qm@web94710.mail.in2.yahoo.com> Message-ID: <667738.27013.qm@web65716.mail.ac4.yahoo.com> Why don't you look?in a histology atlas and try to find out. For your description they can be several things. Ren? J. From: mani kandan To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 26, 2011 12:30 PM Subject: [Histonet] mouse femour section hai, ???? i am working with mouse femours, i found some cells in the section looks like a ring shaped cells i want to know what r they,can u help me in this regard. M.Manikandan, Researcher, Stemcell unit, King Saud university, Riyadh,KSA +966552012697 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu May 26 15:40:57 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu May 26 15:42:05 2011 Subject: [Histonet] RE: off topic/on topic In-Reply-To: <41DD03DE-C258-4FC8-94A9-CD24210147DE@email.arizona.edu> References: <41DD03DE-C258-4FC8-94A9-CD24210147DE@email.arizona.edu> Message-ID: I avoid anything that contains carmine be it yogurt or make up. !! I really don't want bugs eat bugs or put them on my face. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) [algranth@email.arizona.edu] Sent: Thursday, May 26, 2011 3:28 PM To: HISTONET Subject: [Histonet] off topic/on topic This is mostly for the girls - I dropped into the Ulta store yesterday to pick up a few things and the sales person wanted me to try this somewhat new product by Benefit. It was a lip and cheek tint - came in a little bottle - looked like mucicarmine working solution. I looked at the box that it came in and sure enough, one of the first ingredients was CARMINE! So I had to relate a few of the stories about beetles and Carmine and pirates that I learned from reading "A Perfect Red" recommended by Dick Dapson...wonder if I bored her? Maybe she won't bother me the next time I'm rushing through the store trying to get my stuff and get out of there. Andi From azdudley <@t> hotmail.com Thu May 26 16:34:36 2011 From: azdudley <@t> hotmail.com (anita dudley) Date: Thu May 26 16:34:39 2011 Subject: [Histonet] hollande fixative Message-ID: this has come up before but I am going to ask for those of you that use hollande for gastric bxs, do you wash before you put them on the machine? and what do you wash them with? thanks so much!!! anita dudley providence hosp. mobile, ala From mhale <@t> carisls.com Thu May 26 22:51:05 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Thu May 26 22:51:39 2011 Subject: [Histonet] PA HT Position Message-ID: <6F33D8418806044682A391273399860F0848E998@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! We are an 8 physician Dermatology practice located in Bucks County, PA looking for a certified HT or HTL. Candidate must meet CLIA grossing requirements. The candidate will be responsible for routine histology duties. This is a part-time position that offers a competitive salary and flexible hours. Interested applicants should contact Meredith Hale, phone (214)596-2219 or e-mail mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 214-596-7095 mhale@carisls.com From mhale <@t> carisls.com Thu May 26 22:52:36 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Thu May 26 22:53:13 2011 Subject: [Histonet] NV HT Position Message-ID: <6F33D8418806044682A391273399860F0848E999@s-irv-ex301.PathologyPartners.intranet> Great opportunity for a Histotechnician in a brand new laboratory! We are a 5 physician gastroenterology practice located in Las Vegas, NV looking for a certified HT or HTL. Candidate must meet CLIA grossing requirements. The candidate will be responsible for routine histology duties. This is a part-time position that offers a competitive salary and flexible hours. Interested applicants should contact Meredith Hale, phone (214)596-2219 or e-mail mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 214-596-7095 mhale@carisls.com From mab70 <@t> medschl.cam.ac.uk Fri May 27 02:43:03 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri May 27 02:43:27 2011 Subject: [Histonet] off topic/on topic In-Reply-To: <41DD03DE-C258-4FC8-94A9-CD24210147DE@email.arizona.edu> References: <41DD03DE-C258-4FC8-94A9-CD24210147DE@email.arizona.edu> Message-ID: Hi Andi, Would you kindly let me have the reference for "A perfect red", please? Thanks Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: 26 May 2011 20:29 To: HISTONET Subject: [Histonet] off topic/on topic This is mostly for the girls - I dropped into the Ulta store yesterday to pick up a few things and the sales person wanted me to try this somewhat new product by Benefit. It was a lip and cheek tint - came in a little bottle - looked like mucicarmine working solution. I looked at the box that it came in and sure enough, one of the first ingredients was CARMINE! So I had to relate a few of the stories about beetles and Carmine and pirates that I learned from reading "A Perfect Red" recommended by Dick Dapson...wonder if I bored her? Maybe she won't bother me the next time I'm rushing through the store trying to get my stuff and get out of there. Andi From DKBoyd <@t> chs.net Fri May 27 08:25:38 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri May 27 08:25:52 2011 Subject: [Histonet] Unlabeled specimens In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DEBE7C40@LRGHEXVS1.practice.lrgh.org> Message-ID: Irretrivable specimens are not rejected and are reconciled by calling the offending department/ office. The offending person is responsible for coming to the lab and correcting the error. The error is noted on the original Pathology Requistion. The incident is logged into the computer system and reported in the final Pathology report. A Risk Management form is filled out and sent to Risk Manangement. We also perform work for a lab 40 miles away. We have them write an attestation to the fact that they are sure this is the correct patient/information. Sign it and fax it to us. We staple it to the Path. Requisition (requisitions are kept for 2 years). Then we proceed with the steps as stated above. The attending/surgeon is notified by the pathologist. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Podawiltz, Thomas" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/25/2011 01:34 PM To "'Histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] Unlabeled specimens What are your procedures for rejection and correction when you receive either a surgical or Nongyn specimen that has incomplete information on the requisitions or the container is not labeled properly? Do you send it back to the provider's office? Have them come to your lab to make the corrections? Throw away the specimen? Tom THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From c.tague <@t> pathologyarts.com Fri May 27 09:00:45 2011 From: c.tague <@t> pathologyarts.com (Curt Tague) Date: Fri May 27 09:00:55 2011 Subject: [Histonet] NV HT Position In-Reply-To: <6F33D8418806044682A391273399860F0848E999@s-irv-ex301.PathologyPartners.intranet> References: <6F33D8418806044682A391273399860F0848E999@s-irv-ex301.PathologyPartners.intranet> Message-ID: <036401cc1c76$796f55c0$6c4e0140$@tague@pathologyarts.com> Am I the only one who hates seeing posts about POL's. HOW ARE THESE NOT STARK LAW VIOLATIONS? It's self referral, period! As a private lab owner, I'd love to be able to take a few dr. partners, but noooo, that's self referral and against the law. It's all bull, just matters who's contributing more to the campaign funds! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hale, Meredith Sent: Thursday, May 26, 2011 8:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NV HT Position Great opportunity for a Histotechnician in a brand new laboratory! We are a 5 physician gastroenterology practice located in Las Vegas, NV looking for a certified HT or HTL. Candidate must meet CLIA grossing requirements. The candidate will be responsible for routine histology duties. This is a part-time position that offers a competitive salary and flexible hours. Interested applicants should contact Meredith Hale, phone (214)596-2219 or e-mail mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 214-596-7095 mhale@carisls.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michelecarr10 <@t> yahoo.com Fri May 27 10:22:35 2011 From: michelecarr10 <@t> yahoo.com (Michele Carr) Date: Fri May 27 10:22:39 2011 Subject: [Histonet] colloidion Message-ID: <139401.94272.qm@web120718.mail.ne1.yahoo.com> Hi everyone, I am just wondering if any of you out there use this chemical called colloidion.? Our pathologists want to use it for making cell blocks, but looking over the msds, it's not very safe.? If you use it or have used it could you tell me how exactly you stored it and disposed of it. Also if you have any procedure on how to use it to create cell blocks. Thank you all for your help, Michele Carr Medical Laboratory Services From relia1 <@t> earthlink.net Fri May 27 10:30:28 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri May 27 10:30:34 2011 Subject: [Histonet] RELIA Hot Histology Job Alert. RELIA Exclusive - Exciting Opportunity in Charlotte, NC Message-ID: Hi Histonetters! One of my best clients is in need of a full time permanent ASCP certified histotech. Must be qualified to gross. This is one of my best clients because I have placed several people there and they LOVE it. This client has very low turnover and great benefits. If you are interested in hearing more about this opportunity please contact Pam Barker at relia1@earthlink.net or 866-607-3542. Have a Safe, Fun Filled and Happy Memorial Day Weekend. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From andreahooper <@t> rocketmail.com Fri May 27 11:20:49 2011 From: andreahooper <@t> rocketmail.com (andreahooper@rocketmail.com) Date: Fri May 27 11:20:57 2011 Subject: [Histonet] mouse femour section In-Reply-To: <667738.27013.qm@web65716.mail.ac4.yahoo.com> References: <649752.95380.qm@web94710.mail.in2.yahoo.com><667738.27013.qm@web65716.mail.ac4.yahoo.com> Message-ID: <337869993-1306513251-cardhu_decombobulator_blackberry.rim.net-1712807139-@b25.c23.bise6.blackberry> Hi Mani, Chondrocytes? Osteoclasts? Adipocytes? Send a pic and I will try to determine. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Sender: histonet-bounces@lists.utsouthwestern.edu Date: Thu, 26 May 2011 12:58:53 To: mani kandan; histonet@lists.utsouthwestern.edu Reply-To: Rene J Buesa Subject: Re: [Histonet] mouse femour section Why don't you look?in a histology atlas and try to find out. For your description they can be several things. Ren? J. From: mani kandan To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 26, 2011 12:30 PM Subject: [Histonet] mouse femour section hai, ???? i am working with mouse femours, i found some cells in the section looks like a ring shaped cells i want to know what r they,can u help me in this regard. M.Manikandan, Researcher, Stemcell unit, King Saud university, Riyadh,KSA +966552012697 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dholmes <@t> umc.edu Fri May 27 11:47:47 2011 From: dholmes <@t> umc.edu (Dianne E. Holmes) Date: Fri May 27 11:47:54 2011 Subject: [Histonet] MSDS info Message-ID: HELP !!! I am having to list all my chemicals in the lab for the Safety dept. Several have no MSDS sheet with them? (been here longer than I have!) Where can I obtain this info to put in my records? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From c.tague <@t> pathologyarts.com Fri May 27 11:58:25 2011 From: c.tague <@t> pathologyarts.com (Curt Tague) Date: Fri May 27 11:58:33 2011 Subject: [Histonet] MSDS info In-Reply-To: References: Message-ID: <03d101cc1c8f$4b84b0b0$e28e1210$@tague@pathologyarts.com> Call the vendors or check their websites. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dianne E. Holmes Sent: Friday, May 27, 2011 9:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] MSDS info HELP !!! I am having to list all my chemicals in the lab for the Safety dept. Several have no MSDS sheet with them? (been here longer than I have!) Where can I obtain this info to put in my records? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjr6 <@t> psu.edu Fri May 27 12:11:13 2011 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Fri May 27 12:11:37 2011 Subject: [Histonet] RE: MSDS info In-Reply-To: References: Message-ID: The university of Vermont has a very good site for MSDS. I use it for all my chemicals. It is at http://hazard.com/msds/ I have my MSDS in a computer file so if needed I can quickly look up the information a lot easier than when we had paper copies. Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dianne E. Holmes Sent: Friday, May 27, 2011 12:48 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] MSDS info HELP !!! I am having to list all my chemicals in the lab for the Safety dept. Several have no MSDS sheet with them? (been here longer than I have!) Where can I obtain this info to put in my records? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Fri May 27 12:12:29 2011 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri May 27 12:12:44 2011 Subject: [Histonet] RE: off topic for the guys In-Reply-To: <41DD03DE-C258-4FC8-94A9-CD24210147DE@email.arizona.edu> References: <41DD03DE-C258-4FC8-94A9-CD24210147DE@email.arizona.edu> Message-ID: OK, I saw Andrea's post and after all , it is Friday. I saw a recent episode of CSI-New York that had a cochineal beetle as part of the crime scene evidence about a sniper. Anyway, it got me wondering, anyone know if automatic transmission fluid is colored with cochineal (carmine) ?? (I had to make this guy related and don't know any girls working on their transmissions) Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 Learn more about Histotechnology Professionals Day at www.nsh.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Thursday, May 26, 2011 3:29 PM To: HISTONET Subject: [Histonet] off topic/on topic This is mostly for the girls - I dropped into the Ulta store yesterday to pick up a few things and the sales person wanted me to try this somewhat new product by Benefit. It was a lip and cheek tint - came in a little bottle - looked like mucicarmine working solution. I looked at the box that it came in and sure enough, one of the first ingredients was CARMINE! So I had to relate a few of the stories about beetles and Carmine and pirates that I learned from reading "A Perfect Red" recommended by Dick Dapson...wonder if I bored her? Maybe she won't bother me the next time I'm rushing through the store trying to get my stuff and get out of there. Andi From brianj18 <@t> u.washington.edu Fri May 27 12:18:56 2011 From: brianj18 <@t> u.washington.edu (Brian Johnson) Date: Fri May 27 12:19:09 2011 Subject: [Histonet] Histologic Technician 2 Job Opening at University of Washington Message-ID: Histologic Technician 2 Job Requisition 72777 University of Washington Department of Comparative Medicine The department's Histology and Imaging Core is currently seeking a full-time temporary employee for a histologic technician 2 position. The candidate will be working as part of a close-knit team supporting research projects from various sources within and external to the university. You can access the University of Washington Careers site by going to: http://www.washington.edu/admin/hr/jobs/apl/index.html If you are an Internal Applicant click the "UW Employee Login" link. If you are an External Applicant click the "External Applicant Login" link. Type 72777 in the Req # box to reach the full job description. Thank you. From shive003 <@t> umn.edu Fri May 27 13:05:28 2011 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri May 27 13:05:31 2011 Subject: [Histonet] MSDS info References: Message-ID: <5AD0C0BCBBA94648B716FBA35D4D3F36@auxs.umn.edu> If you have no luck with calling your vendors or finding an MSDS link on their website, simply Google your chemical name and the letters MSDS after it (such as: sodium chloride MSDS). Up will pop a long list of companies who have them available. Find the format you like the best and print out for your files. This should do, in lieu of one from the actual vendor, as long as the CAS number is the same as you use in the lab. Jan Shivers UMN ----- Original Message ----- From: "Dianne E. Holmes" To: Sent: Friday, May 27, 2011 11:47 AM Subject: [Histonet] MSDS info HELP !!! I am having to list all my chemicals in the lab for the Safety dept. Several have no MSDS sheet with them? (been here longer than I have!) Where can I obtain this info to put in my records? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Fri May 27 13:39:59 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri May 27 13:39:58 2011 Subject: [Histonet] RE: off topic for the guys In-Reply-To: References: <41DD03DE-C258-4FC8-94A9-CD24210147DE@email.arizona.edu>, Message-ID: <008E5EF6-F373-47CA-A869-663E0FE0B324@mimectl> believe it is Solvent Red 164 (Oil Red B) that is used to color ATF Ronnie Houston ________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie [dellav@musc.edu] Sent: Friday, May 27, 2011 1:12 PM To: Grantham, Andrea L - (algranth); HISTONET Subject: [Histonet] RE: off topic for the guys OK, I saw Andrea's post and after all , it is Friday. I saw a recent episode of CSI-New York that had a cochineal beetle as part of the crime scene evidence about a sniper. Anyway, it got me wondering, anyone know if automatic transmission fluid is colored with cochineal (carmine) ?? (I had to make this guy related and don't know any girls working on their transmissions) Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 Learn more about Histotechnology Professionals Day at www.nsh.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Thursday, May 26, 2011 3:29 PM To: HISTONET Subject: [Histonet] off topic/on topic This is mostly for the girls - I dropped into the Ulta store yesterday to pick up a few things and the sales person wanted me to try this somewhat new product by Benefit. It was a lip and cheek tint - came in a little bottle - looked like mucicarmine working solution. I looked at the box that it came in and sure enough, one of the first ingredients was CARMINE! So I had to relate a few of the stories about beetles and Carmine and pirates that I learned from reading "A Perfect Red" recommended by Dick Dapson...wonder if I bored her? Maybe she won't bother me the next time I'm rushing through the store trying to get my stuff and get out of there. Andi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From rjbuesa <@t> yahoo.com Fri May 27 14:16:19 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 27 14:16:23 2011 Subject: [Histonet] MSDS info In-Reply-To: References: Message-ID: <877789.80820.qm@web65707.mail.ac4.yahoo.com> "Google" the name of the reagent and you will get the MSDS Re From: Dianne E. Holmes To: "Histonet@lists.utsouthwestern.edu" Sent: Friday, May 27, 2011 12:47 PM Subject: [Histonet] MSDS info HELP !!! I am having to list all my chemicals in the lab for the Safety dept.? Several have no MSDS sheet with them?? (been here longer than I have!)? Where can I obtain this info to put in my records? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Fri May 27 14:20:13 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri May 27 14:20:19 2011 Subject: [Histonet] Brady labeling system Message-ID: <9BF995BC0E47744E9673A41486E24EE238D5A04123@MERCERMAIL.MercerU.local> I would like to talk to anyone who uses the Brady Specimen labeling system for cassettes and slides. Please contact me via this email address or by phone number below. Thanks for your help in advance. Shirley Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From azdudley <@t> hotmail.com Fri May 27 14:26:44 2011 From: azdudley <@t> hotmail.com (anita dudley) Date: Fri May 27 14:26:49 2011 Subject: [Histonet] cassette and slide labelling Message-ID: I have another question. what cassette and slide labeling systems are people using out there. we have 50 to 60 case load a day. thanks, just wondered what people use. thanks again, anita, providence hosp. mobile, alabama From patpxs <@t> gmail.com Fri May 27 14:33:14 2011 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Fri May 27 14:33:17 2011 Subject: [Histonet] colloidion In-Reply-To: <139401.94272.qm@web120718.mail.ne1.yahoo.com> References: <139401.94272.qm@web120718.mail.ne1.yahoo.com> Message-ID: Is there any reason why the pathologist can't used agar? It's very simple to use and non-toxic. -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Fri, May 27, 2011 at 11:22 AM, Michele Carr wrote: > Hi everyone, I am just wondering if any of you out there use this chemical > called colloidion.? Our pathologists want to use it for making cell blocks, but > looking over the msds, it's not very safe.? If you use it or have used it could > you tell me how exactly you stored it and disposed of it. Also if you have any > procedure on how to use it to create cell blocks. > Thank you all for your help, > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Barry.R.Rittman <@t> uth.tmc.edu Fri May 27 18:22:46 2011 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri May 27 18:22:50 2011 Subject: [Histonet] colloidion In-Reply-To: References: <139401.94272.qm@web120718.mail.ne1.yahoo.com>, Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E9018D864C55@UTHCMS1.uthouston.edu> Michele Are you sure that he wanted to use collodion.? If so you may want to ask his reasons for using this. Tissues processed in collodion take a long time to process, lot of technical skill in cutting. Also the nail in the project perhaps is that it is very expensive and difficult to transport because of special handling precautions. Trust me you do not want the hassles with this material. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello [patpxs@gmail.com] Sent: Friday, May 27, 2011 2:33 PM To: Michele Carr Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] colloidion Is there any reason why the pathologist can't used agar? It's very simple to use and non-toxic. -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Fri, May 27, 2011 at 11:22 AM, Michele Carr wrote: > Hi everyone, I am just wondering if any of you out there use this chemical > called colloidion. Our pathologists want to use it for making cell blocks, but > looking over the msds, it's not very safe. If you use it or have used it could > you tell me how exactly you stored it and disposed of it. Also if you have any > procedure on how to use it to create cell blocks. > Thank you all for your help, > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mfisher <@t> ecrmc.org Fri May 27 19:30:28 2011 From: mfisher <@t> ecrmc.org (Marcia Fisher) Date: Fri May 27 19:30:40 2011 Subject: [Histonet] A Perfect Red Message-ID: http://www.amybutlergreenfield.com/News.html Great reading! ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Friday, May 27, 2011 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 90, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Unlabeled Specimens (Marcia Fisher) 2. off topic/on topic (Grantham, Andrea L - (algranth)) 3. Re: mouse femour section (Rene J Buesa) 4. RE: off topic/on topic (McMahon, Loralee A) 5. hollande fixative (anita dudley) 6. PA HT Position (Hale, Meredith) 7. NV HT Position (Hale, Meredith) 8. RE: off topic/on topic (Margaret Blount) 9. Re: Unlabeled specimens (DKBoyd@chs.net) 10. RE: NV HT Position (Curt Tague) 11. colloidion (Michele Carr) 12. RELIA Hot Histology Job Alert. RELIA Exclusive - Exciting Opportunity in Charlotte, NC (Pam Barker) 13. Re: mouse femour section (andreahooper@rocketmail.com) 14. MSDS info (Dianne E. Holmes) 15. RE: MSDS info (Curt Tague) ---------------------------------------------------------------------- Message: 1 Date: Thu, 26 May 2011 17:22:41 +0000 From: Marcia Fisher Subject: [Histonet] Unlabeled Specimens To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" If the specimen is in-house, we walk it back to the department for correction. If outside the hospital, the courier returns it to the clinic/doctor's office for correction. All mislabeled specimens, however, have a form filled out (Specimen Rejection form), "copies" of the specimen container are made and a report made to QRM is severe enough to warrant. This is all in accordance with CAP. Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? ------------------------------ Message: 2 Date: Thu, 26 May 2011 12:28:38 -0700 From: "Grantham, Andrea L - (algranth)" Subject: [Histonet] off topic/on topic To: HISTONET Message-ID: <41DD03DE-C258-4FC8-94A9-CD24210147DE@email.arizona.edu> Content-Type: text/plain; charset="us-ascii" This is mostly for the girls - I dropped into the Ulta store yesterday to pick up a few things and the sales person wanted me to try this somewhat new product by Benefit. It was a lip and cheek tint - came in a little bottle - looked like mucicarmine working solution. I looked at the box that it came in and sure enough, one of the first ingredients was CARMINE! So I had to relate a few of the stories about beetles and Carmine and pirates that I learned from reading "A Perfect Red" recommended by Dick Dapson...wonder if I bored her? Maybe she won't bother me the next time I'm rushing through the store trying to get my stuff and get out of there. Andi ------------------------------ Message: 3 Date: Thu, 26 May 2011 12:58:53 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] mouse femour section To: mani kandan , "histonet@lists.utsouthwestern.edu" Message-ID: <667738.27013.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Why don't you look?in a histology atlas and try to find out. For your description they can be several things. Ren? J. From: mani kandan To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 26, 2011 12:30 PM Subject: [Histonet] mouse femour section hai, ???? i am working with mouse femours, i found some cells in the section looks like a ring shaped cells i want to know what r they,can u help me in this regard. M.Manikandan, Researcher, Stemcell unit, King Saud university, Riyadh,KSA +966552012697 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 26 May 2011 16:40:57 -0400 From: "McMahon, Loralee A" Subject: [Histonet] RE: off topic/on topic To: "Grantham, Andrea L - (algranth)" , HISTONET Message-ID: Content-Type: text/plain; charset="us-ascii" I avoid anything that contains carmine be it yogurt or make up. !! I really don't want bugs eat bugs or put them on my face. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) [algranth@email.arizona.edu] Sent: Thursday, May 26, 2011 3:28 PM To: HISTONET Subject: [Histonet] off topic/on topic This is mostly for the girls - I dropped into the Ulta store yesterday to pick up a few things and the sales person wanted me to try this somewhat new product by Benefit. It was a lip and cheek tint - came in a little bottle - looked like mucicarmine working solution. I looked at the box that it came in and sure enough, one of the first ingredients was CARMINE! So I had to relate a few of the stories about beetles and Carmine and pirates that I learned from reading "A Perfect Red" recommended by Dick Dapson...wonder if I bored her? Maybe she won't bother me the next time I'm rushing through the store trying to get my stuff and get out of there. Andi ------------------------------ Message: 5 Date: Thu, 26 May 2011 16:34:36 -0500 From: anita dudley Subject: [Histonet] hollande fixative To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" this has come up before but I am going to ask for those of you that use hollande for gastric bxs, do you wash before you put them on the machine? and what do you wash them with? thanks so much!!! anita dudley providence hosp. mobile, ala ------------------------------ Message: 6 Date: Thu, 26 May 2011 22:51:05 -0500 From: "Hale, Meredith" Subject: [Histonet] PA HT Position To: Message-ID: <6F33D8418806044682A391273399860F0848E998@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Great opportunity for a Histotechnician in a brand new laboratory! We are an 8 physician Dermatology practice located in Bucks County, PA looking for a certified HT or HTL. Candidate must meet CLIA grossing requirements. The candidate will be responsible for routine histology duties. This is a part-time position that offers a competitive salary and flexible hours. Interested applicants should contact Meredith Hale, phone (214)596-2219 or e-mail mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 214-596-7095 mhale@carisls.com ------------------------------ Message: 7 Date: Thu, 26 May 2011 22:52:36 -0500 From: "Hale, Meredith" Subject: [Histonet] NV HT Position To: Message-ID: <6F33D8418806044682A391273399860F0848E999@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Great opportunity for a Histotechnician in a brand new laboratory! We are a 5 physician gastroenterology practice located in Las Vegas, NV looking for a certified HT or HTL. Candidate must meet CLIA grossing requirements. The candidate will be responsible for routine histology duties. This is a part-time position that offers a competitive salary and flexible hours. Interested applicants should contact Meredith Hale, phone (214)596-2219 or e-mail mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 214-596-7095 mhale@carisls.com ------------------------------ Message: 8 Date: Fri, 27 May 2011 08:43:03 +0100 From: "Margaret Blount" Subject: RE: [Histonet] off topic/on topic To: "Grantham, Andrea L - (algranth)" , "HISTONET" Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi Andi, Would you kindly let me have the reference for "A perfect red", please? Thanks Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: 26 May 2011 20:29 To: HISTONET Subject: [Histonet] off topic/on topic This is mostly for the girls - I dropped into the Ulta store yesterday to pick up a few things and the sales person wanted me to try this somewhat new product by Benefit. It was a lip and cheek tint - came in a little bottle - looked like mucicarmine working solution. I looked at the box that it came in and sure enough, one of the first ingredients was CARMINE! So I had to relate a few of the stories about beetles and Carmine and pirates that I learned from reading "A Perfect Red" recommended by Dick Dapson...wonder if I bored her? Maybe she won't bother me the next time I'm rushing through the store trying to get my stuff and get out of there. Andi ------------------------------ Message: 9 Date: Fri, 27 May 2011 09:25:38 -0400 From: DKBoyd@chs.net Subject: Re: [Histonet] Unlabeled specimens To: "Podawiltz, Thomas" Cc: "'Histonet@lists.utsouthwestern.edu'" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Irretrivable specimens are not rejected and are reconciled by calling the offending department/ office. The offending person is responsible for coming to the lab and correcting the error. The error is noted on the original Pathology Requistion. The incident is logged into the computer system and reported in the final Pathology report. A Risk Management form is filled out and sent to Risk Manangement. We also perform work for a lab 40 miles away. We have them write an attestation to the fact that they are sure this is the correct patient/information. Sign it and fax it to us. We staple it to the Path. Requisition (requisitions are kept for 2 years). Then we proceed with the steps as stated above. The attending/surgeon is notified by the pathologist. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Podawiltz, Thomas" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/25/2011 01:34 PM To "'Histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] Unlabeled specimens What are your procedures for rejection and correction when you receive either a surgical or Nongyn specimen that has incomplete information on the requisitions or the container is not labeled properly? Do you send it back to the provider's office? Have them come to your lab to make the corrections? Throw away the specimen? Tom THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 10 Date: Fri, 27 May 2011 07:00:45 -0700 From: "Curt Tague" Subject: RE: [Histonet] NV HT Position To: "'Hale, Meredith'" , Message-ID: <036401cc1c76$796f55c0$6c4e0140$@tague@pathologyarts.com> Content-Type: text/plain; charset="us-ascii" Am I the only one who hates seeing posts about POL's. HOW ARE THESE NOT STARK LAW VIOLATIONS? It's self referral, period! As a private lab owner, I'd love to be able to take a few dr. partners, but noooo, that's self referral and against the law. It's all bull, just matters who's contributing more to the campaign funds! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hale, Meredith Sent: Thursday, May 26, 2011 8:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NV HT Position Great opportunity for a Histotechnician in a brand new laboratory! We are a 5 physician gastroenterology practice located in Las Vegas, NV looking for a certified HT or HTL. Candidate must meet CLIA grossing requirements. The candidate will be responsible for routine histology duties. This is a part-time position that offers a competitive salary and flexible hours. Interested applicants should contact Meredith Hale, phone (214)596-2219 or e-mail mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 214-596-7095 mhale@carisls.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 27 May 2011 08:22:35 -0700 (PDT) From: Michele Carr Subject: [Histonet] colloidion To: histonet@lists.utsouthwestern.edu Message-ID: <139401.94272.qm@web120718.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi everyone, I am just wondering if any of you out there use this chemical called colloidion.? Our pathologists want to use it for making cell blocks, but looking over the msds, it's not very safe.? If you use it or have used it could you tell me how exactly you stored it and disposed of it. Also if you have any procedure on how to use it to create cell blocks. Thank you all for your help, Michele Carr Medical Laboratory Services ------------------------------ Message: 12 Date: Fri, 27 May 2011 11:30:28 -0400 From: "Pam Barker" Subject: [Histonet] RELIA Hot Histology Job Alert. RELIA Exclusive - Exciting Opportunity in Charlotte, NC To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters! One of my best clients is in need of a full time permanent ASCP certified histotech. Must be qualified to gross. This is one of my best clients because I have placed several people there and they LOVE it. This client has very low turnover and great benefits. If you are interested in hearing more about this opportunity please contact Pam Barker at relia1@earthlink.net or 866-607-3542. Have a Safe, Fun Filled and Happy Memorial Day Weekend. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 13 Date: Fri, 27 May 2011 16:20:49 +0000 From: andreahooper@rocketmail.com Subject: Re: [Histonet] mouse femour section To: "mani kandan" , "histonet@lists.utsouthwestern.edu" Message-ID: <337869993-1306513251-cardhu_decombobulator_blackberry.rim.net-1712807139-@b25.c23.bise6.blackberry> Content-Type: text/plain; charset="Windows-1252" Hi Mani, Chondrocytes? Osteoclasts? Adipocytes? Send a pic and I will try to determine. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Sender: histonet-bounces@lists.utsouthwestern.edu Date: Thu, 26 May 2011 12:58:53 To: mani kandan; histonet@lists.utsouthwestern.edu Reply-To: Rene J Buesa Subject: Re: [Histonet] mouse femour section Why don't you look?in a histology atlas and try to find out. For your description they can be several things. Ren? J. From: mani kandan To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 26, 2011 12:30 PM Subject: [Histonet] mouse femour section hai, ???? i am working with mouse femours, i found some cells in the section looks like a ring shaped cells i want to know what r they,can u help me in this regard. M.Manikandan, Researcher, Stemcell unit, King Saud university, Riyadh,KSA +966552012697 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Fri, 27 May 2011 11:47:47 -0500 From: "Dianne E. Holmes" Subject: [Histonet] MSDS info To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" HELP !!! I am having to list all my chemicals in the lab for the Safety dept. Several have no MSDS sheet with them? (been here longer than I have!) Where can I obtain this info to put in my records? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ------------------------------ Message: 15 Date: Fri, 27 May 2011 09:58:25 -0700 From: "Curt Tague" Subject: RE: [Histonet] MSDS info To: "'Dianne E. Holmes'" , Message-ID: <03d101cc1c8f$4b84b0b0$e28e1210$@tague@pathologyarts.com> Content-Type: text/plain; charset="us-ascii" Call the vendors or check their websites. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dianne E. Holmes Sent: Friday, May 27, 2011 9:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] MSDS info HELP !!! I am having to list all my chemicals in the lab for the Safety dept. Several have no MSDS sheet with them? (been here longer than I have!) Where can I obtain this info to put in my records? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 90, Issue 33 **************************************** ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.   From sadey <@t> hotmail.ca Fri May 27 20:44:28 2011 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Fri May 27 20:44:32 2011 Subject: [Histonet] back up power for VIPs? Message-ID: Hello everyone: We are experiencing lots of quick power outages at work that keeps setting the processors into "power outage" alarms. Can anyone recommend a back up power product. I was told we can't put them on battery back up b/c of their power requirements??? They are on emergency power. Thanks for your help in advance. :) Sheila From rjbuesa <@t> yahoo.com Sat May 28 09:50:40 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat May 28 09:50:44 2011 Subject: [Histonet] back up power for VIPs? In-Reply-To: References: Message-ID: <951765.28742.qm@web65709.mail.ac4.yahoo.com> You need a dedicated electrical outlet from your building to your tissue processor. This can be done easily. Ren? J. From: Sheila Adey To: histonet@lists.utsouthwestern.edu Sent: Friday, May 27, 2011 9:44 PM Subject: [Histonet] back up power for VIPs? Hello everyone: We are experiencing lots of quick power outages at work that keeps setting the processors into "power outage" alarms. Can anyone recommend a back up power product. I was told we can't put them on battery back up b/c of their power requirements??? They are on emergency power. Thanks for your help in advance. :) Sheila ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From coralmani <@t> yahoo.co.in Sat May 28 12:11:58 2011 From: coralmani <@t> yahoo.co.in (mani kandan) Date: Sat May 28 12:12:03 2011 Subject: [Histonet] Need second hand instruments Message-ID: <392730.56640.qm@web94708.mail.in2.yahoo.com> hai histonetters, ???????? i am planing to set up a histology lab in my country(india), so i need second hand instruments like microtome, embedding stage,incubator. if you have please give for a price or gift. i am looking for favourable reply. thank u M.Manikandan, Researcher, Stemcell unit, King Saud university, Riyadh,KSA +966552012697 From bakevictoria <@t> gmail.com Sat May 28 12:20:34 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Sat May 28 12:20:37 2011 Subject: [Histonet] back up power for VIPs? In-Reply-To: References: Message-ID: Shelia- I'm hoping that I'm reading this correctly. You're currently on emergency or designated line power, but you're experiencing power fluctuations which trip up the processors. If this is the case, I have encountered this and it turned out that the engineering department was working on changing out generators - but didn't inform us. They were doing it on what they considered off hours on the weekends right around the time our processors would be just starting to process. If you haven't check with them yet I would and let them know what is happening. When it first started to happen the electrical people came and checked the outlets - which of course, showed no problems. They never told us about the generator changes, I had to get a little ugly to find that out. Not knowing what processors you have I can't answer about the back up power. I know that it is possible to purchase stand alone generators that can be installed and will provide power in emergency situations but they are expensive and have limitations as to how long they can sustain power. It's been about two years since I looked into them so things may have changed. Hope this of some help. Vikki On Fri, May 27, 2011 at 9:44 PM, Sheila Adey wrote: > > Hello everyone: > > We are experiencing lots of quick power outages at work that keeps setting > the processors into "power outage" alarms. > Can anyone recommend a back up power product. I was told we can't put them > on battery back up b/c of their power requirements??? > They are on emergency power. > > Thanks for your help in advance. :) > > Sheila > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Sun May 29 11:01:10 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun May 29 11:01:15 2011 Subject: [Histonet] Need second hand instruments In-Reply-To: <392730.56640.qm@web94708.mail.in2.yahoo.com> References: <392730.56640.qm@web94708.mail.in2.yahoo.com> Message-ID: <513044.74554.qm@web65701.mail.ac4.yahoo.com> As far as I know you in India manufacture all types of histology instruments with acceptable quality and cheap. Have you tried? your sources. Sending you an used instrument from USA I think will be more expensive than buying one used in India. You could also try eBay. Ren? J. From: mani kandan To: histonet@lists.utsouthwestern.edu Sent: Saturday, May 28, 2011 1:11 PM Subject: [Histonet] Need second hand instruments hai histonetters, ???????? i am planing to set up a histology lab in my country(india), so i need second hand instruments like microtome, embedding stage,incubator. if you have please give for a price or gift. i am looking for favourable reply. thank u M.Manikandan, Researcher, Stemcell unit, King Saud university, Riyadh,KSA +966552012697 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From coralmani <@t> yahoo.co.in Sun May 29 15:52:47 2011 From: coralmani <@t> yahoo.co.in (mani kandan) Date: Sun May 29 15:52:53 2011 Subject: [Histonet] antigen retreval method-mouse femour Message-ID: <154057.5214.qm@web94716.mail.in2.yahoo.com> hai, ??? i am working with mouse femours, can any one have a experience in immunohistochemistry on mouse femours,because after antigen retreval they cancellous bone not looks good,morphology of cells also changed, can anyone tell me about good antigen retrieval?method. M.Manikandan, Researcher, Stemcell unit, King Saud university, Riyadh,KSA +966552012697 From k84as <@t> yahoo.com Sun May 29 17:44:57 2011 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Sun May 29 17:45:00 2011 Subject: [Histonet] Alcian blue quistion Message-ID: <793415.3600.qm@web112618.mail.gq1.yahoo.com> hi every body i have a confusing quistion reagrding Alcian blue pH 1 & 2.5 stains. suppose you have 100 goblet cell react positive to pH 1 which mean all have sulfated mucin. and 50 cell from other section react positive to pH 2.5 which mean acidic type(sulfated or sialated). why these 50 cell only react to pH 2.5 althogh the other non reacted cells possess acidic sulfated type?? on other word i need to know for what type of mucins alcian B 2.5 react with? ? thanx all From anonwums1 <@t> gmail.com Sun May 29 18:42:05 2011 From: anonwums1 <@t> gmail.com (Adam .) Date: Sun May 29 18:42:10 2011 Subject: [Histonet] antigen retreval method-mouse femour In-Reply-To: <154057.5214.qm@web94716.mail.in2.yahoo.com> References: <154057.5214.qm@web94716.mail.in2.yahoo.com> Message-ID: Hi Mani, This is a very common problem for bone IHC, and there is no perfect solution. Here is what I do: after cutting the paraffin embedded, fixed and decalcified sections, I dry the slides sitting flat in a 37C oven for 5 or more days. The longer you dry them, the better your results. Then I retrieve with citrate buffer pH 6.0 for 10 mins at 95C. The bone generally stays intact, although the morphology is rarely as nice as unretrieved sections. Some people swear by other antigen retrieval methods that don't involve heat, but I've never managed to get them to work. Adam On Sun, May 29, 2011 at 3:52 PM, mani kandan wrote: > > hai, > i am working with mouse femours, can any one have a experience in > immunohistochemistry on mouse femours,because after antigen retreval they > cancellous bone not looks good,morphology of cells also changed, can anyone > tell me about good antigen retrieval method. > > M.Manikandan, > Researcher, > Stemcell unit, > King Saud university, > Riyadh,KSA > +966552012697 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Mon May 30 09:56:37 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 30 09:56:42 2011 Subject: [Histonet] antigen retreval method-mouse femour In-Reply-To: <154057.5214.qm@web94716.mail.in2.yahoo.com> References: <154057.5214.qm@web94716.mail.in2.yahoo.com> Message-ID: <9903.77439.qm@web65712.mail.ac4.yahoo.com> I have to assume that you have decalcified the bone before doing anything else, right? Ren? J. From: mani kandan To: histonet@lists.utsouthwestern.edu Sent: Sunday, May 29, 2011 4:52 PM Subject: [Histonet] antigen retreval method-mouse femour hai, ??? i am working with mouse femours, can any one have a experience in immunohistochemistry on mouse femours,because after antigen retreval they cancellous bone not looks good,morphology of cells also changed, can anyone tell me about good antigen retrieval?method. M.Manikandan, Researcher, Stemcell unit, King Saud university, Riyadh,KSA +966552012697 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Mon May 30 10:47:21 2011 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Mon May 30 10:47:38 2011 Subject: [Histonet] Colloidin? No!! Message-ID: <8CDECF7FF91A6EC-678-5396F@webmail-m033.sysops.aol.com> Michele Carr asks about using colloidin for cell block preparation. Years ago, histology labs would coat the inside of a glass test tube will colloidin (maybe 1% dissolved in 50:50 absolute alcohol and ether) and allow it to dry. When they needed to make a cell block, they poured the solution containing the cells in the test tube and spun it down. The aquous solution caused the colloidin to separate from the glass. you could pour off the solution and wrap the cell pellet up in the softened celloidin and put it in a cassette for routine processing. However, times have changed! Using celloidin (nitrated cellulose or gun cotton) is dangerous and the alcohol ether solvent is also dangerous. I think cytology labs use a jell called "histogel" or something in which they capture cell blocks. Maybe there is a decent cytology lab in your area that uses this product or a similier one you can try. Michael Titford USA Pathology Mobile AL USA From mtitford <@t> aol.com Mon May 30 10:54:15 2011 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Mon May 30 10:54:33 2011 Subject: [Histonet] More about "A perfect red" Message-ID: <8CDECF8F6030DB6-678-53C72@webmail-m033.sysops.aol.com> The full citation is "A perfect red: Empire, espionage, and the quest for the color of desire." By Amy Butler Greenfield. Harper Collins. (It was $26.95 in 2005 but now you may be able to pick up a good used copy online). Michael Titford USA Pathology Mobile AL USA From rsrichmond <@t> gmail.com Mon May 30 12:44:30 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon May 30 12:44:35 2011 Subject: [Histonet] Re: celloidin, collodion Message-ID: I've never seen celloidin, collodion, or what have you - solutions of cellulose nitrate in ethyl ether or other flammable solvents - used for cytologic cell block preparation. Cellulose nitrate is simply too much of a fire and explosion hazard to allow it in a histology lab at all. Thermo Scientific Richard-Allan Scientific - or whatever they're called this week - offers HistoGel, a proprietary gel for cell block preparation. I've seen it used, and at least in some labs it works well. It's supposed to be quite expensive, in an area where the bean counters come down hard. HistoGel requires formaldehyde fixation. Many people used to use trypticase soy agar (TSA) purloined from the microbiology lab, but I'm not sure they use it any more. An old technique perhaps stiil in use is adding plasma to the cell block pellet, and then clotting the plasma with thrombin. I don't have any experience with it. People have a lot of trouble preparing cell blocks. Bob Richmond Samurai Pathologist Knoxville TN From rosie_scrimizzi <@t> hotmail.com Mon May 30 19:39:33 2011 From: rosie_scrimizzi <@t> hotmail.com (Rosie Scrimizzi) Date: Mon May 30 19:39:38 2011 Subject: [Histonet] Re: celloidin, collodion In-Reply-To: References: Message-ID: for plasma cell blocks, ensure cell pellet is "dry" (tap off as much supernatent so as not to dilute plasma), use Na Citrate plasma (still has fibrinogen and Ca ++ ions from ie coagulation studies tube obtained from your haematology dept.), add 2 drops to your cell pellet, mix and add one drop of thrombin....works a treat, used this method for 20 yrs, no failures. Fix cell block in 10%NBF or whatever fixative you use and process. need to refrigerate plasma and thrombin. Also need to use clean pipette to dispense plasma so as not to contaminate it. Obtain fresh plasma weekly ( good nutrient media for bacteria) Rosie Scrimizzi Technical Specialist Histology Siemens HDx > Date: Mon, 30 May 2011 13:44:30 -0400 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: celloidin, collodion > > I've never seen celloidin, collodion, or what have you - solutions of > cellulose nitrate in ethyl ether or other flammable solvents - used > for cytologic cell block preparation. > > Cellulose nitrate is simply too much of a fire and explosion hazard to > allow it in a histology lab at all. > > Thermo Scientific Richard-Allan Scientific - or whatever they're > called this week - offers HistoGel, a proprietary gel for cell block > preparation. I've seen it used, and at least in some labs it works > well. It's supposed to be quite expensive, in an area where the bean > counters come down hard. HistoGel requires formaldehyde fixation. > > Many people used to use trypticase soy agar (TSA) purloined from the > microbiology lab, but I'm not sure they use it any more. > > An old technique perhaps stiil in use is adding plasma to the cell > block pellet, and then clotting the plasma with thrombin. I don't have > any experience with it. > > People have a lot of trouble preparing cell blocks. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Tue May 31 03:18:28 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Tue May 31 03:20:11 2011 Subject: [Histonet] MSDS info In-Reply-To: References: Message-ID: Your suppliers are required by law to supply msds and they would no doubt be please to do so. However the Sigma website is great as you can search all their reagents and click on MSDS and download a PDF file of the MSDS. http://www.sigmaaldrich.com/ This hyperlink should take you to their site. Regards Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dianne E. Holmes Sent: 27 May 2011 17:48 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] MSDS info HELP !!! I am having to list all my chemicals in the lab for the Safety dept. Several have no MSDS sheet with them? (been here longer than I have!) Where can I obtain this info to put in my records? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Tue May 31 03:24:13 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Tue May 31 03:25:07 2011 Subject: [Histonet] a Perfect Red Message-ID: Dear All, Thank you all for your contributions to my request for information about "A Perfect Red", Yes I should have searched it on the Net. Just didn't think! Anyway I shall be ordering it and will enjoy reading it. Thank you all a million. Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 From TMcNemar <@t> lmhealth.org Tue May 31 05:22:14 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue May 31 05:22:18 2011 Subject: [Histonet] back up power for VIPs? In-Reply-To: References: Message-ID: Hello Sheila, We are using the Smart-UPS 2200 from APC. We are also on a dedicated emergency line through our facility. We are running the Sakura VIP 5. I cannot give you any other specifics about the UPS since I can't get to the back of it but it was recommended and installed by our biomed people. Seems to work fine. That model has been discontinued but this is the recommended replacement... APC Smart-UPS 2200VA USB & Serial 120V More Images APC Smart-UPS, 1980 Watts / 2200 VA,Input 120V / Output 120V, Interface Port DB-9 RS-232, SmartSlot Includes: CD with software, Smart http://www.apc.com/products/resource/include/techspec_index.cfm?base_sku=SUA2200 Hope this helps... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Friday, May 27, 2011 9:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back up power for VIPs? Hello everyone: We are experiencing lots of quick power outages at work that keeps setting the processors into "power outage" alarms. Can anyone recommend a back up power product. I was told we can't put them on battery back up b/c of their power requirements??? They are on emergency power. Thanks for your help in advance. :) Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From brianj18 <@t> u.washington.edu Tue May 31 10:31:58 2011 From: brianj18 <@t> u.washington.edu (Brian Johnson) Date: Tue May 31 10:32:09 2011 Subject: [Histonet] Histologic Technician 2 Job Opening at University of Washington Message-ID: <4B6C431E-BA3D-4509-A217-F649777AA49A@u.washington.edu> Histologic Technician 2 Job Requisition 72777 University of Washington Department of Comparative Medicine The department's Histology and Imaging Core is currently seeking a full-time temporary employee for a histologic technician 2 position. The candidate will be working as part of a close-knit team supporting research projects from various sources within and external to the university. You can access the University of Washington Careers site by going to: http://www.washington.edu/admin/hr/jobs/apl/index.html If you are an Internal Applicant click the "UW Employee Login" link. If you are an External Applicant click the "External Applicant Login" link. Type 72777 in the Req # box to reach the full job description. Thank you. From fricton <@t> umn.edu Tue May 31 11:28:13 2011 From: fricton <@t> umn.edu (Jennifer Fricton) Date: Tue May 31 11:28:32 2011 Subject: [Histonet] Help with Masson's Trichrome - not working on cryo sections Message-ID: Hello, I am completely stumped by a problem we?ve been having with our Masson?s Trichrome. It isn?t working properly on cryo sections. We developed our protocol several years ago and it has been a standard in our lab without any trouble, until about four months ago when it just stopped working on frozen sections. It still works fine on paraffin sections. You can see images at this link: http://www.med.umn.edu/lhi/prod/groups/med/@pub/@med/@dom/@lhi/documents/con tent/med_content_340494.pdf What seems to be happening is that the beibrich scarlet + acid fuchsin reagent is either not staining the muscle tissue, or is washing out of the tissue. The aniline blue is still staining the connective tissue very well (it stopped working, too, but we solved by reducing time in water rinse following stain). My Masson?s protocol is very standard ? essentially straight out of the Armed Forces Institute of Pathology Manual (p. 94). I fix my cryosections in 10% NBF x 30 mins. before starting the protocol. I?ve made sure my formalin is fresh and properly stored, with no white precipitate. I?ve tried the Bouin?s mordant step at both 56C and room temp overnight. I?ve reduced my distilled water rinses following the biebrich scarlet and aniline blue steps to just a quick dip, thinking maybe I was de-staining too much before setting the stains with acetic acid. I?ve re-made all solutions, checked all components and pH levels. I haven?t done anything different with my frozen tissues, and I?ve tried several different freshly cut tissues. Has anyone seen this before and have any tips on what is going wrong? Your help is greatly appreciated! -- Jennifer L. Fricton, B.S. Scientist, Lab Manager Lillehei Heart Institute Histology & Microscopy Core Facility University of Minnesota School of Medicine, Division of Cardiology 312 Church Street SE 4-290 Nils Hasselmo Hall Minneapolis, MN 55455 Lab: 612-626-3090 From mw <@t> personifysearch.com Tue May 31 12:41:30 2011 From: mw <@t> personifysearch.com (Matt Ward) Date: Tue May 31 12:41:36 2011 Subject: [Histonet] HOT New Histology Opportunity in Baltimore and DC!! Message-ID: <122c4335c4fda7c3cc590fd9ee46af17@mail.gmail.com> Histonet, We are currently searching for a histology laboratory professional who would be interested in working in the Field for a global manufacturer of Histology and IHC equipment. The position offers a very strong Base Salary + Bonus + Car Allowance + Full Benefits. This position does not require previous field experience and is perfect for a histotech who is interested in opportunities outside of the lab. Please contact me directly at mw@personifysearch.com to learn more. Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com *Field Support Specialist* *The Company:* Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. *Primary Responsibilities:* The primary responsibility of this role will be to provide in-field technical applications support for current and next generation range of products. The FSS will install/set-up instrumentation in customer laboratories, perform demonstrations and maintain demonstration equipment in a clean and operational manner. The FSS will also train customers on the use of instrumentation. Conducting in-field post purchase applications support and performing instrument validations will be a key responsibility of this position. Additional Responsibilities: - Build an in-depth understanding of new product technologies and their applications in a diagnostic environment - Contribute to the strategic planning process by providing technical data on instrumentation and reagents - Conduct post-purchase reagent order management - Be the customer liaison by providing scientific and laboratory expertise for in-field installations, evaluations, training and trouble-shooting - Provide customer support for remote problem solving - Design and perform experiments to investigate and solve tough technical applications problems From jdooley2008 <@t> yahoo.com Tue May 31 14:04:42 2011 From: jdooley2008 <@t> yahoo.com (James Dooley) Date: Tue May 31 14:04:45 2011 Subject: [Histonet] INFAR-1 & KGF antibodies Message-ID: <297657.82294.qm@web45916.mail.sp1.yahoo.com> Hello, I am looking for an antibody to use for INFAR-1 (interferon alpha receptor) to use on fresh frozen mouse tissue sections. If anyone can recommend any antibodies and the fixation used it would be much appreciated. Best regards, James From robin_dean <@t> compbio.com Tue May 31 18:07:51 2011 From: robin_dean <@t> compbio.com (Robin Dean) Date: Tue May 31 18:11:13 2011 Subject: [Histonet] re-using modified Davidson's solution Message-ID: <003301cc1fe7$90ccf780$b266e680$@com> Hi All, Can one re-use modified Davidson's solution for fixing eyes and testes, or has it lost its potency/efficacy after being used previously? Can you rejuvenate it by adding more acetic acid? (how much?) I appreciate any helpful comments. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist & Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_dean@compbio.com From AnthonyH <@t> chw.edu.au Tue May 31 20:55:34 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue May 31 20:55:45 2011 Subject: [Histonet] Help with Masson's Trichrome - not working on cryo sections In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715718858898@xmdb02.nch.kids> Jennifer, Looking at the images, is it possible that the slides were air-dried for too long prior to staining? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Fricton Sent: Wednesday, 1 June 2011 2:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help with Masson's Trichrome - not working on cryo sections Hello, I am completely stumped by a problem we?ve been having with our Masson?s Trichrome. It isn?t working properly on cryo sections. We developed our protocol several years ago and it has been a standard in our lab without any trouble, until about four months ago when it just stopped working on frozen sections. It still works fine on paraffin sections. You can see images at this link: http://www.med.umn.edu/lhi/prod/groups/med/@pub/@med/@dom/@lhi/documents/con tent/med_content_340494.pdf What seems to be happening is that the beibrich scarlet + acid fuchsin reagent is either not staining the muscle tissue, or is washing out of the tissue. The aniline blue is still staining the connective tissue very well (it stopped working, too, but we solved by reducing time in water rinse following stain). My Masson?s protocol is very standard ? essentially straight out of the Armed Forces Institute of Pathology Manual (p. 94). I fix my cryosections in 10% NBF x 30 mins. before starting the protocol. I?ve made sure my formalin is fresh and properly stored, with no white precipitate. I?ve tried the Bouin?s mordant step at both 56C and room temp overnight. I?ve reduced my distilled water rinses following the biebrich scarlet and aniline blue steps to just a quick dip, thinking maybe I was de-staining too much before setting the stains with acetic acid. I?ve re-made all solutions, checked all components and pH levels. I haven?t done anything different with my frozen tissues, and I?ve tried several different freshly cut tissues. Has anyone seen this before and have any tips on what is going wrong? Your help is greatly appreciated! -- Jennifer L. Fricton, B.S. Scientist, Lab Manager Lillehei Heart Institute Histology & Microscopy Core Facility University of Minnesota School of Medicine, Division of Cardiology 312 Church Street SE 4-290 Nils Hasselmo Hall Minneapolis, MN 55455 Lab: 612-626-3090 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. 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