[Histonet] GFP labelled cells after decalcification...are they still labelled?

[James Hart]

Hi All

A first time user of Histonet here -with a quick question for research
histologists...

Has anyone ever attempted to locate GFP expressing cells in
bone-marrow sections after decalcification and paraffin embedding? (I guess
my question really is: Does the decalcification process destroy or weaken
the GFP signal)

Any advice would be greatly appreciated!

Regards

James

Dr James Hart BVSc MS
Comparative Orthopaedics, Cornell University