[Histonet] RE: Microsatellite Instability Antibodies

[Patsy Ruegg]

I use the NovaCastra MSH-2 and MLH-1 on the Leica Bond Maxx.
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Thursday, March 24, 2011 9:49 AM
To: Hoekert, W.E.J.; Evans, Andria B; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Microsatellite Instability Antibodies

We use Leica Bond Max and Biocare antibodies work very well for us. We're
working on perfecting the Leica antibodies as well.  J

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 

 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hoekert,
W.E.J.
Sent: Thursday, March 24, 2011 11:18
To: Evans, Andria B; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Microsatellite Instability Antibodies

>We are currently running the Ventana IHC stainers with the UltraView
Detection.
 
 
Just like us!
 
MLH-1: Cell Marque (ref 760-4264). 30' cc1, 32' ab incubation.
MSH-2: Cell Marque (ref 760-4265).  60' cc1, 60' ab inc.
PMS2: Becton Dickinson, clone A16-4, cat nr 556415. 30' cc1, 32' ab inc.
+amplification
MSH6: Becton Dickinson, clone 44, cat nr 610919. 60' cc2, 60' ab inc. 
 
MSH-6 does not give very nice results, however, it works.
 
Good Luck,
 
Willem

________________________________

Van: histonet-bounces <@t> lists.utsouthwestern.edu namens Evans, Andria B
Verzonden: do 24-3-2011 13:31
Aan: histonet <@t> lists.utsouthwestern.edu
Onderwerp: [Histonet] Microsatellite Instability Antibodies



I am currently working up the MSI Antibodies (MLH1, MSH6, MSH2, and PMS2)
and need some input on the protocol that you may be running in your lab and
what clone(manufacture) you are using.

We are currently running the Ventana IHC stainers with the UltraView
Detection.

Thanks for your help!

Andria B Evans HTL(ASCP)CM

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