[Histonet] RE: Stupid Rabbit Primary Antibodies

[Goins, Tresa]

You might try Background Sniper from Biocare or Background Buster from Innovex - we process animal tissues exclusively and we use several rabbit primary antibodies.


Tresa Goins, Ph.D.
Histopathology Supervisor
Department of Livestock
Bozeman, Montana



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of sgoebel <@t> mirnarx.com
Sent: Tuesday, March 22, 2011 3:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Stupid Rabbit primaries!

So I haven't had to deal with rabbit polyclonal primaries in a long time
because I remember how much the background sucks with them.
Unfortunately the only available antibody is a rabbit polyclonal.  Does
anyone have any suggestions for how to eliminate the background?  I have
diluted almost to the point of the antigens not showing!  Thanks guys
and gals!!

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

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