[Histonet] RE: Verhoeff/Masson's Stain

[John Shelley]

Hi Shirley,

I do make it fresh each time and the reagents are brand new from the company. I am using an appendix as my control and trying to get my hands on some aorta but I really think I should see some elastin fiber staining in the appendix.

Kind Regards!
 
John J Shelley

-----Original Message-----
From: Shirley A. Powell [mailto:POWELL_SA <@t> mercer.edu] 
Sent: Tuesday, March 22, 2011 2:04 PM
To: John Shelley
Subject: RE: Verhoeff/Masson's Stain

Your problem may be that the elastic stain should be made fresh every time, or you are differentiating in ferric chloride too long.  Does the elastic stain get mixed right before use?  Also check the expiration date of the kit. 
Shirley

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John Shelley
Sent: Tuesday, March 22, 2011 1:59 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Verhoeff/Masson's Stain

Hello Histonetters,

I have been asked to work on a project that will require me to do a Verhoeff/Masson's stain. I have run some samples through the stain from a protocol that I found on the archives and it is not working to my satisfaction. I am not getting the nice elastin fibers to show black like they should. I have looked at the slides just before going into 2% ferric chloride and it appears to be dull or limited staining. I am using a kit from PolyScientific and not sure if that might be my problem or not. My question is, are there another people who are running this special stain combo and would you be willing to share the procedure and also who you are buying your reagents from to accomplish the staining. I appreciate any help with this request. Thanks!!!

Kind Regards!

John J Shelley


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