[Histonet] how to bleach melanoma cases without bad effect on IHC

[Sebree Linda A]

Sabeti,

If you have the option of using a detection system with a chromogen other than DAB, i.e. Alkaline Phosphatase Red, that is by far the easiest way to get around the pigment issue. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Shahram Sabeti
Sent: Tuesday, March 22, 2011 3:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] how to bleach melanoma cases without bad effect on IHC

hello dear fellows,
   i have an IHC project on paraffin embedded melanoma cases; some of them being heavily pigmented. i have seen many protocols based on H2O2 to bleach the pigmented ones, but i am not sure about any possible effect on antigen retrieval.please guide me .
yours,
sabeti


      
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