[Histonet] Bielschowskys Stain

[Molinari, Betsy]

Glawon,
Thank you for your help. I appreciate it.
Betsy

-----Original Message-----
From: Glawon Flood [mailto:smoothw <@t> gmail.com] 
Sent: Tuesday, March 15, 2011 2:33 PM
To: Molinari, Betsy
Subject: Re: [Histonet] Bielschowskys Stain

Betsy,

I don't know of any modifications for Eager's but there are modifications for the Fink-Heimer technique as well as the Nauta-Gygax technique that can be used on paraffin-embedded tissue. I've only come across these in passing and you may have to do a little digging to find a protocol. 

I hope the info proves useful...good luck!

Glawon Flood, HTL (ASCP)


Won

On Mar 15, 2011, at 11:43 AM, "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu> wrote:

> Thank you for your response. I "googled" Eagers and found a protocol for Eagers for frozen sections. I have paraffin blocks. 
> Do you know if there is and Eagers method for paraffin?
> Thank you so much.
> Betsy
> 
> -----Original Message-----
> From: Glawon Flood [mailto:smoothw <@t> gmail.com] 
> Sent: Tuesday, March 15, 2011 10:22 AM
> To: Molinari, Betsy
> Subject: Re: [Histonet] Bielschowskys Stain
> 
> Hi Betsy, 
> 
> Let me start off by saying that I am by no means an expert on what's preferred. However, in my experience, I've come to find the Bielschowsky method very useful for staining nerve fibers, but I would not consider it so for demonstrating axonal degeneration. I would suggest that you maybe consider Eager's method for degenerating axons. Unlike Bielschowsky's, Eager's actually distinguishes those fibers that are normal from those that are degenerating. 
> 
> I hope this helps.
> 
> Glawon Flood, HTL (ASCP)
> 
> On Mar 15, 2011, at 6:00 AM, "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu> wrote:
> 
>> Hi ,
>> An investigator would like  show axonal degeneration in peripheral nerves. Is Bielschowsky's  the preferred stain?
>> Thanks!
>> 
>> 
>> Betsy Molinari   HT(ASCP)
>> Texas Heart Institute
>> Cardiovascular Pathology
>> 6770 Bertner Ave.
>> MC 1-283
>> Houston, TX 77030
>> 832-355-6524
>> 832-355-6812 (fax)
>> 
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