[Histonet] PAS for basement membrane&In-Reply-To=

[Ruppert, Amysue]

Yes, it is good to use the metabisulfite rinses to remove any excess schiff reagent after the schiff step. But after the metabisulfite rinses, you still need to go into TAP water for several minutes to have the full color of the PAS reaction develop. It will get much brighter after the final tap water rinse.

amysue

______________________________________________________________________
The contents of this message may contain private, protected and/or privileged information.  If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within.  Please contact the sender and advise of the erroneous delivery by return e-mail or telephone.  Thank you for your cooperation.