[Histonet] PAS for basement membrane

[Jennifer MacDonald]

Try decreasing the time in periodic acid.  Basement membrane will over 
oxidize faster than the fungus.  Some docs like the over-oxidation to 
high-light the fungus.




"Martin, Erin" <Erin.Martin <@t> ucsf.edu> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/15/2011 07:19 AM

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[Histonet] PAS for basement membrane






Hi all,

I'm having a problem with my PAS stain.  My pathologist says that glycogen 
and fungus stain beautifully but basement membranes are pale.  We are 
using 0.5% periodic acid for 10 minutes and schiff's for 25 minutes 
followed by 5 min rinse in warm/hot water.  Any thoughts?  Thank you in 
advance!

Thanks
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

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