[Histonet] g ratio

[Jay Lundgren]

Bonjour,

  I think a Luxol Fast Blue is the standard for visualizing the myelin
sheath. Here's a free tip; leave your slides in the LFB stain overnight,
instead of the shorter time specified in most protocols.  You're going
to decolorize them anyway.  Decolorize the slides until you don't see the
stain run off anymore.  You get a really brilliant LFB this way. The
Pathologists I've worked with love it.  Comment dit on "myelin sheath" en
Francaise?



         Vive
l'esprit de Lafayette!


Jay A. Lundgren M.S., HTL(ASCP)