[Histonet] Fwd: GMS counterstain
Cheryl Crowder
ccrowder <@t> vetmed.lsu.edu
Fri Mar 11 10:27:39 CST 2011
Ally - I have worked with several pathologists who were color-blind -one to
greens; one to blues. As a result we counterstained the GMS with different
colors. A counterstain is just that. It gives a color to secondary tissue
elements. Light greens, hematoxylin, metanil yellow and nuclear fast red
were used. All worked well, photographed well and were the choice of the
pathologist. Many older pathologists "grew up" with the procedure outlined
in the AFIP manual which stated green. But other pathologists are more
amiable to trying other colors. Maybe you should just as them what they
prefer.
Cheryl
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