[Histonet] Control Slides

[Debra Siena]

One of the things that I usually suggest is that when every time that you cut the control, sequentially number the slides and test the first section and the last to make sure that you have not cut through the area of interest and keep that slide as part of the quality assurance/quality control.  If both sections fit, you could even put on the same slide and mark which is which for cost and space savings.  

Debbie Siena HT(ASCP)QIHC
Technical Manager | StatLab Medical Products
Direct: 972-436-1010  x229 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony Henwood
Sent: Thursday, March 10, 2011 4:11 PM
To: 'Sheila Fonner'; 'Amador, Amanda'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Control Slides

Sheila,
I believe that Amanda is referring to control slides cut for the Steiner Modification of the Warthin-Starry stain not for immunohistochemistry.
I would be surprised if the control slides for the Steiner's would "go off". It is possible that the technique has gone out of control.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila Fonner
Sent: Thursday, 10 March 2011 11:53 PM
To: 'Amador, Amanda'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Control Slides

Amanda,

You will lose antigenicity on the spirochete control slides after that long of a period.  We usually cut no more than 10 in advance, and we keep them in the refrigerator.  Hope this helps.

Sheila HT (ASCP)
Knoxville Dermatopathology Lab
Knoxville, TN


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amador, Amanda
Sent: Wednesday, March 09, 2011 10:22 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Control Slides

Is there guidelines for how long special stains controls are good for once they are cut?  We have spirochetes for our Steiner that is from 2007 and we are having issues.

Amanda Amador, HT(ASCP)CM
AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | Indianapolis, IN  46219 | phone 317.275.8052 | aamador <@t> ameripath.com<mailto:aamador <@t> ameripath.com> | www.AmeriPath.com<http://www.ameripath.com/>

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