[Histonet] RE: cell blocks

[sgoebel <@t> mirnarx.com]

I actually fixed the cells without the agar overnight, and left them in
the agar overnight...

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

-----Original Message-----
From: Tony Henwood [mailto:AnthonyH <@t> chw.edu.au] 
Sent: Tuesday, March 08, 2011 6:43 PM
To: Sarah Goebel; histonet <@t> lists.utsouthwestern.edu
Subject: RE: cell blocks

Sarah,

It is important that the agar cell block is allowed to fix in formalin
(10%NBF) before wrapping in paper, at least 2 hours. It will hold its
shape better.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
sgoebel <@t> mirnarx.com
Sent: Wednesday, 9 March 2011 2:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cell blocks

So...I am trying to make a cell block using 0.9% agar.  I have done this
in the past with no problems.  It was at a different facility and I
don't know if the agar is the same?  I used bacto-agar this time, but
last week someone tried to do my protocol with agarose and the "boogers"
dried up and fell out of the block.  The only other difference is we
have clear rite xylene substitute in this processor where the other one
I used had xylene.  Tissues processed on the same cycle come out fine
with the clear rite so I don't think that it the problem.  When the cell
blocks come out they are as thin as the papers I wrapped them in making
it almost impossible to see and to subsequently cut.  Any suggestions
for making cell blocks without using the plasma/thrombin method or the
insanely expensive histo-gel would be appreciated.  

Second, does anyone have a suggestion on how to repair the cells that
are embedded in the tissue paper thick "cell block"?

Thanks guys and gals!

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

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