[Histonet] SOX11 antibody

[Connolly, Brett M]

Hi Martha,
I haven't used it, but you can find some info on it from the human
protein atlas here:

http://www.proteinatlas.org/ENSG00000176887/antibody

They recommend 1:100 and you can check out Sigma's recommended procedure
for using their Prestige antibodies here:

http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/
1/ihc_procedure.Par.0001.File.tmp/ihc_procedure.pdf


Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martha
Ward
Sent: Thursday, March 03, 2011 1:54 PM
To: Histonet
Subject: [Histonet] SOX11 antibody

Hello all,

I have been asked to try to work up the SOX11 antibody for a research
project.   The antibody they gave me is from Sigma Life Science.  I am
having difficulty getting any staining at all.   If anyone has any
suggestions I would be very grateful.    We are using a Leica Bond 3.
Thanks in advance for any help.


Martha Ward, MT (ASCP) QIHC
Assistant Manager
Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27157
336-716-2104

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