[Histonet] Mouse cardiac myosin antibodies

[Liz Chlipala]

Mark

I took a brief look at the abcam antibody and in particular the review
with the paraffin staining.  If I was purchasing the antibody I would
question the results of that review, since they used a goat anti-mouse
detection system which is not ideal for mouse tissue.  Plus I'm not sure
that the staining pattern is consistent with what I would expect.  I
would expect to see staining of the myofibrils within the cell.  It
appears from the image to be staining nuclei and the outside of the
cell. I would call Abcam to see if they have tested this antibody
internally for paraffin processing or did they completely rely on that
review.  You could also try enzyme retrieval rather than HIER.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mark
Elliott
Sent: Wednesday, March 02, 2011 3:59 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mouse cardiac myosin antibodies

I have been asked to stain mouse hearts for cardiac myosin. I have tried
the antibody from Abcam (ab50967-Mouse monoclonal to heavy chain cardiac
myosin) with no luck. I am using the Mouse on Mouse kit from Biocare and
it worked well for me for some other mouse antibodies on mouse tissue
but doesn't want to work on this antibody.  I have tried different
retrieval solutions (pH6 and pH9, as well as Biocare rodent Decloaker).
Anyone use this antibody?  Any suggestions for another antibody greatly
appreciated.
 
Thanks
Mark

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