AW: [Histonet] Sentinel Lymp Node Procedure

[Gudrun Lang]

For breast sentinels:

Sentinels are received fresh.
Sentinels below 4 mm diameter are cut in two halfes - one for frozens, one
for paraffin.
Sentinels bigger than 4 mm diameter are cut in 2 mm slices - the
middle-slice for frozens, the rest for paraffin.

Frozens: 3 sections; 1. and 3. for HE; the middle for Fast-CK-Pan
Paraffin: sections each 250 µm, first and last section for HE, the sections
between for CK-Pan immunohisto.

Positiv lymphnodes found in frozens -- then only two sections for HE.

Gudrun Lang
Histolab, Austria



-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Scott,
Allison D
Gesendet: Dienstag, 01. März 2011 19:10
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Sentinel Lymp Node Procedure

Hello to all in histoland.  Does anyone have a procedure for sentinel
lymph nodes that they would be willing to share.  I am revamoing mine.
We are in our CAP window.  Looking for them in the next 2 weeks.  Any
help will be appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
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