From hctrupath <@t> att.net Tue Mar 1 07:23:15 2011 From: hctrupath <@t> att.net (Heather Cooper) Date: Tue Mar 1 07:23:19 2011 Subject: [Histonet] HT Exam Message-ID: <699025.70625.qm@web80007.mail.sp1.yahoo.com> Hello everyone!!!!!!!!! Has anyone taken the HT Exam recently. I heard it was hard. Can anyone give me any help please. Heather Cooper TruPath Laboratory, LLC Leesville, LA From cpyse <@t> x-celllab.com Tue Mar 1 07:50:35 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Mar 1 07:52:02 2011 Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? In-Reply-To: References: Message-ID: <000601cbd817$a4a252e0$ede6f8a0$@com> Practice, practice, practice! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine h&e slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.....tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NKonop <@t> chw.org Tue Mar 1 08:13:55 2011 From: NKonop <@t> chw.org (Konop, Nicole) Date: Tue Mar 1 08:14:01 2011 Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? In-Reply-To: <000601cbd817$a4a252e0$ede6f8a0$@com> References: <000601cbd817$a4a252e0$ede6f8a0$@com> Message-ID: I agree with Cynthia. Practice and setting up a standard system of how you cut. I do the same thing every time with each block I cut. Cutting faster is not necessarily a good thing if you are jeopardizing quality just to cut more slides. It's quality over quantity in my book any day! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, March 01, 2011 7:51 AM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Practice, practice, practice! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine h&e slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.....tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Tue Mar 1 08:26:25 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Mar 1 08:26:40 2011 Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? In-Reply-To: References: <000601cbd817$a4a252e0$ede6f8a0$@com> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DE133D95@LRGHEXVS1.practice.lrgh.org> Worry more about quality than speed at this time. I measure my staff on quality rather than how fast things get done. It's a waste of time, energy and resources to have to re-cut blocks because of poor quality. Speed comes with time and experience. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, March 01, 2011 9:14 AM To: histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Cynthia. Practice and setting up a standard system of how you cut. I do the same thing every time with each block I cut. Cutting faster is not necessarily a good thing if you are jeopardizing quality just to cut more slides. It's quality over quantity in my book any day! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, March 01, 2011 7:51 AM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Practice, practice, practice! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine h&e slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.....tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Janice.Mahoney <@t> alegent.org Tue Mar 1 08:33:19 2011 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Mar 1 08:33:28 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per hour? In-Reply-To: References: Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7404@EXCHMBC2.ad.ah.local> Hello Jenny, An experienced tech should be able to cut and average of 1 block every 2.5 minutes. Embedding is 1 block per minute ave. Note this is an average. It includes difficult and easy tissue, cleaning the waterbath after each block, writing/printing the slide number, etc. There may be variations in each lab that can change these numbers. Hope this helps. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 4:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine h&e slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.....tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From PAMarcum <@t> uams.edu Tue Mar 1 08:58:20 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Mar 1 08:56:40 2011 Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DE133D95@LRGHEXVS1.practice.lrgh.org> References: <000601cbd817$a4a252e0$ede6f8a0$@com> , <38667E7FB77ECD4E91BFAEB8D986386323DE133D95@LRGHEXVS1.practice.lrgh.org> Message-ID: I agree with Tom. Janice is correct on her timing numbers. However; when you are starting out it is best to get the flow for working at your station than beating a clock. You will gain your speed with learning accuracy which is more important for the patient at the end. We are in patient care not NASCAR and should always remember patient first, which translates to accuracy and the best sections we can produce on every slide. Speed will come as you do more blocks and get better. Pam Marcum ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas [tpodawiltz@lrgh.org] Sent: Tuesday, March 01, 2011 8:26 AM To: 'Konop, Nicole'; histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Worry more about quality than speed at this time. I measure my staff on quality rather than how fast things get done. It's a waste of time, energy and resources to have to re-cut blocks because of poor quality. Speed comes with time and experience. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, March 01, 2011 9:14 AM To: histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Cynthia. Practice and setting up a standard system of how you cut. I do the same thing every time with each block I cut. Cutting faster is not necessarily a good thing if you are jeopardizing quality just to cut more slides. It's quality over quantity in my book any day! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, March 01, 2011 7:51 AM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Practice, practice, practice! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine h&e slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.....tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From Janice.Mahoney <@t> alegent.org Tue Mar 1 09:02:17 2011 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Mar 1 09:03:01 2011 Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? In-Reply-To: References: <000601cbd817$a4a252e0$ede6f8a0$@com> , <38667E7FB77ECD4E91BFAEB8D986386323DE133D95@LRGHEXVS1.practice.lrgh.org> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7406@EXCHMBC2.ad.ah.local> Well said Pam. All the speed in the world means nothing if you can't produce quality slides. The suggestion to do things the same way every time is a good one. However, learning what works well on what tissue is very helpful too. Like icing something that wrinkles or soaking something that shatters, etc. It all comes from experience. I plan to retire in 2 months but will keep my foot in the door working casual so that I don't lose my skills. I love this profession! Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 01, 2011 8:58 AM To: Podawiltz, Thomas; 'Konop, Nicole'; histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Tom. Janice is correct on her timing numbers. However; when you are starting out it is best to get the flow for working at your station than beating a clock. You will gain your speed with learning accuracy which is more important for the patient at the end. We are in patient care not NASCAR and should always remember patient first, which translates to accuracy and the best sections we can produce on every slide. Speed will come as you do more blocks and get better. Pam Marcum ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas [tpodawiltz@lrgh.org] Sent: Tuesday, March 01, 2011 8:26 AM To: 'Konop, Nicole'; histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Worry more about quality than speed at this time. I measure my staff on quality rather than how fast things get done. It's a waste of time, energy and resources to have to re-cut blocks because of poor quality. Speed comes with time and experience. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, March 01, 2011 9:14 AM To: histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Cynthia. Practice and setting up a standard system of how you cut. I do the same thing every time with each block I cut. Cutting faster is not necessarily a good thing if you are jeopardizing quality just to cut more slides. It's quality over quantity in my book any day! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, March 01, 2011 7:51 AM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Practice, practice, practice! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine h&e slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.....tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From cmiller <@t> physlab.com Tue Mar 1 09:12:45 2011 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Mar 1 09:12:51 2011 Subject: [Histonet] looking for KJ Kellogg Message-ID: I am looking for KJ Kellogg, or Kjirsten Kellogg she is a HT/PA ASCP working some where in Kansas I think. KJ is originally from Wisconsin. I have an urgent, personal message for her. Thanks, Cheri Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor, Safety Officer, Physicians Laboratory Services Omaha, NE. 68127 402 731 4145 ext. 554 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From jqb7 <@t> cdc.gov Tue Mar 1 10:29:07 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Mar 1 10:29:48 2011 Subject: [Histonet] Slide filing system search Message-ID: Hi all, We are looking for a system of some sort that will allow us to group interesting cases together for quick review. We will use this for teaching, visiting scientists, etc. We want something that will allow for the slides to be stored flat and not in a vertical position, if at all possible. Anyone know of anything? We are talking about quite a few cases...maybe 100+. Vendors welcome to reply. Thanks in advance! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From tjrichmond <@t> hughes.net Tue Mar 1 10:35:22 2011 From: tjrichmond <@t> hughes.net (Tonia Richmond) Date: Tue Mar 1 10:35:29 2011 Subject: [Histonet] Labelers - Needs and Must Haves Message-ID: <110122392.12944.1298997322264.JavaMail.mail@webmail20> If you had the chance to pick features that are a MUST HAVE for cassette and slide labelers what would those features/must haves be? Curious to Know Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From laurie.colbert <@t> huntingtonhospital.com Tue Mar 1 10:50:01 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Mar 1 10:50:06 2011 Subject: [Histonet] Labelers - Needs and Must Haves In-Reply-To: <110122392.12944.1298997322264.JavaMail.mail@webmail20> References: <110122392.12944.1298997322264.JavaMail.mail@webmail20> Message-ID: <57BE698966D5C54EAE8612E8941D768301269027@EXCHANGE3.huntingtonhospital.com> The ability to print barcodes and read barcodes -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tonia Richmond Sent: Tuesday, March 01, 2011 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Labelers - Needs and Must Haves If you had the chance to pick features that are a MUST HAVE for cassette and slide labelers what would those features/must haves be? Curious to Know Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 1 10:51:01 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 1 10:51:04 2011 Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? In-Reply-To: Message-ID: <244978.10684.qm@web65712.mail.ac4.yahoo.com> To cut 24 single slides blcoks/hour Ren? J. --- On Mon, 2/28/11, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? To: histonet@lists.utsouthwestern.edu Date: Monday, February 28, 2011, 5:18 PM I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine h&e slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.....tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hardwired <@t> techie.com Tue Mar 1 11:01:58 2011 From: hardwired <@t> techie.com (hardwired@techie.com) Date: Tue Mar 1 11:02:23 2011 Subject: [Histonet] I.F. Controls Message-ID: <8CDA649826C080C-1124-905E@web-mmc-m05.sysops.aol.com> Hi everyone, Just wondering what you all use as controls for I.F. kindney biopsies!! Thanks D From wdesalvo.cac <@t> hotmail.com Tue Mar 1 11:03:16 2011 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Mar 1 11:03:20 2011 Subject: [Histonet] Labelers - Needs and Must Haves In-Reply-To: <110122392.12944.1298997322264.JavaMail.mail@webmail20> References: <110122392.12944.1298997322264.JavaMail.mail@webmail20> Message-ID: We just went through an evaluation for cassette printers that produce a 2D bar code. Top features we looked for: Print Accession#, last name/first initial, and 2D bar code under 10 seconds a cassette Minimal jamming/defects of printed cassettes, 1 defect/100 cassette or less Tubed or taped cassettes for hoppers for quick and easy loading Cost of cassettes available for use time and complexity of daily, weekly, monthly maintenance of instrument Ease of use by employees Interfaced to multiple LIS Compatible with work flow software and vendor supported We found that the cassette printers fell into three are types of printers available, tape/ribbon transfer, ink jet and laser etching. All three types have there pluses and minuses. William DeSalvo, B.S., HTL(ASCP) > Date: Tue, 1 Mar 2011 16:35:22 +0000 > From: tjrichmond@hughes.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Labelers - Needs and Must Haves > > > If you had the chance to pick features that are a MUST HAVE for > cassette and slide labelers what would those features/must haves be? > Curious to Know > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message.. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hctrupath <@t> att.net Tue Mar 1 11:50:40 2011 From: hctrupath <@t> att.net (Heather Cooper) Date: Tue Mar 1 11:50:44 2011 Subject: [Histonet] HELP!!!!! H. Pylori Immunos Message-ID: <762291.55285.qm@web80007.mail.sp1.yahoo.com> Does anyone do H. Pylori Immunos by hand? From JMitchell <@t> uwhealth.org Tue Mar 1 12:00:44 2011 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Tue Mar 1 12:00:50 2011 Subject: [Histonet] NSH - One Day VIR Forum & VIR Teleconference Series Message-ID: <2108AECB05DBFF48A9C436A79215574006207DCD@UWHC-MAIL03.uwhis.hosp.wisc.edu> One Day VIR Forum Saturday, March 26, 2011 < 8:30 am - 4:15 pm Hampton Inn & Suites in Raleigh, NC Sessions Include: * Digital Pathology - A New Era for Pathology presented by Curtis Adams, PhD, Sr. Product Manager, Life Sciences, Aperio * Developing, Trouble Shooting, and Optimizing IHC Detection Systems for Non-Human Tissues presented by Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC, Manager Premier Laboratory, LLC * Laser Microdissection: The Before and After presented by Diane L. Sterchi, MS, HT(ASCP)HTL, Sr. Research Associate, Covance, Inc. * Tissue Grossing & Pathology: Descriptive Methods & Terminology presented by Richard French, DVM, PhD, Sr. Veterinary Pathologist, University of New Hampshire Registration Fee: $119 (NSH member); $159 (non members) For workshop abstracts and registration information: http://www.goeshow.com/nsh/VIR/ereg607300.cfm?clear VIR Teleconference Summer Series NSH teleconferences are a great, inexpensive way to provide continuing education to a large number of employees. The cost for each teleconference is the same regardless of the number of attendees. Topics include: * June 29th: Selection, Production and Characterization of Probes for In Situ Hybridization presented by Stephanie Villarreal, Merck * July 20th: Working Up a New Antibody in Animal Tissue Sections presented by Elizabeth A. Chlipala, Premier Laboratory, LLC * August 17th: Resin Histology: A Practical Approach for Demonstrating Undemineralized Bone presented by Jack Ratliff, BioMimetic Therapeutics, Inc. Registration Fee: $125.00 Per Teleconference: includes materials and sign in sheets, audio CD and test (good for contact hour credit for 2 years); Order the entire VIR Summer Series by June 28, 2011 and save $100.00! For abstracts and registration information: https://www.goeshow.com/nsh/VIRTeleconferences/ereg416386.cfm?pg=home Any questions please contact the NSH Office, 443-535-4060. From gayle.callis <@t> bresnan.net Tue Mar 1 12:00:14 2011 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Mar 1 12:01:12 2011 Subject: [Histonet] Charles (Chuck) Churukian Remembered Message-ID: <000701cbd83a$8624e9e0$926ebda0$@callis@bresnan.net> With sadness, I received this message from National Society of Histotechnology about the passing of Chuck Churukian and was asked to pass it on to Histonet. Charles J. Churukian Brighton: May 11, 1928 - February 23, 2011, Charles J. Churukian 82. Predeceased by his parents, Joseph and Christin Aintablian Churukian, and sister, Sally Churukian Taroni. He is survived by his wife, Irene Billings Churukian of 41 years and his sister, Rose Churukian Milone as well as sisters-in-law, nieces and nephews. Charles will be sadly missed by family and friends. As a PFC in the infantry, he served as a heavy machine gunner in World War II receiving the Army of Occupation Medal and Victory Medal. Teacher, mentor, poet, editor, innovator, "guru of special stains", Charles worked in histology laboratories for 54 years and supervised the Histotechnology Lab at the UR Medical Center for the last 40 years. He was recently presented with the "Histotechnologist of the Decade" Award by the National Society of Histotechnology for his contributions to the laboratory science field having numerous publications, presentations and awards to his credit. He devoted his career looking for ways to modify the art of special stains for the benefit of patient care. In addition to his professional life, he offered spiritual guidance to many inmates at the jail. On Saturday, March 5, at 11:00, friends are invited to attend a Memorial Service at Asbury United Methodist Church at 1050 East Ave. followed by a reception in 1010 East Avenue, adjacent to the church. Interment, White Haven at the convenience of the family. Charles' family would like to thank the staff at UR Wilmot Cancer Center, and LifetimeCare Agency for their loving compassionate care In lieu of flowers, donations may be directed to Good News and Jail Ministry of the Rochester Area, AMAA Scholarship Fund (for university students in Armenia), Asbury Outreach Programs (Dining Caring Center, Storehouse) or James P. Wilmot Cancer Center. Gayle M. Callis HTL,HT,MT(ASCP) Bozeman MT From kblack <@t> digestivehlth.com Tue Mar 1 12:02:24 2011 From: kblack <@t> digestivehlth.com (Konni Black) Date: Tue Mar 1 12:02:44 2011 Subject: [Histonet] HT/HTL needed in St. Augustine, FL Message-ID: A friend has asked me to post a request for an HT/HTL for a GI lab in St. Augustine, FL. For more information, please contact Robin Scott at rscott@gi-associates.com. Thank you From dmccaig <@t> ckha.on.ca Tue Mar 1 12:07:55 2011 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Tue Mar 1 12:08:01 2011 Subject: [Histonet] negative controls on immunos Message-ID: If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana From Allison_Scott <@t> hchd.tmc.edu Tue Mar 1 12:10:15 2011 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Tue Mar 1 12:10:19 2011 Subject: [Histonet] Sentinel Lymp Node Procedure Message-ID: <1872B4A455B7974391609AD8034C79FC026DFD76@LBEXCH01.hchd.local> Hello to all in histoland. Does anyone have a procedure for sentinel lymph nodes that they would be willing to share. I am revamoing mine. We are in our CAP window. Looking for them in the next 2 weeks. Any help will be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Mar 1 12:30:21 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Mar 1 12:31:21 2011 Subject: [Histonet] RE: negative controls on immunos In-Reply-To: References: Message-ID: We do not run additional negatives with each run on the same block. If it was a different block then yes we would run a negative. It takes up too many spaces on the machine and uses reagents. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig [dmccaig@ckha.on.ca] Sent: Tuesday, March 01, 2011 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls on immunos If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Mar 1 12:32:43 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Mar 1 12:33:20 2011 Subject: [Histonet] HELP!!!!! H. Pylori Immunos In-Reply-To: <762291.55285.qm@web80007.mail.sp1.yahoo.com> References: <762291.55285.qm@web80007.mail.sp1.yahoo.com> Message-ID: We usually run them on our automated stainers but I have been known to do them by hand in a pinch Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather Cooper [hctrupath@att.net] Sent: Tuesday, March 01, 2011 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP!!!!! H. Pylori Immunos Does anyone do H. Pylori Immunos by hand? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Tue Mar 1 12:42:20 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Mar 1 12:42:23 2011 Subject: [Histonet] HELP!!!!! H. Pylori Immunos In-Reply-To: References: <762291.55285.qm@web80007.mail.sp1.yahoo.com> Message-ID: I've done them by hand, what's your question? ??????? Jay A. Lundgren M.S., HTL (ASCP) From settembr <@t> umdnj.edu Tue Mar 1 12:52:43 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Tue Mar 1 12:52:52 2011 Subject: [Histonet] RE: negative controls on immunos In-Reply-To: References: Message-ID: Yes, you should run negative controls with each run - today and tomorrow. Something can happen in tomorrow's run so tomorrow's negative control will have different issues from today's negative control. The negative control should go through everything that your slides go through, whether it's today's great run or tomorrows electrical black out or whatever mishap. Whenever you run a test you must have a positive control and a negative control. Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ 07103 USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Tuesday, March 01, 2011 1:30 PM To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: negative controls on immunos We do not run additional negatives with each run on the same block. If it was a different block then yes we would run a negative. It takes up too many spaces on the machine and uses reagents. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig [dmccaig@ckha.on.ca] Sent: Tuesday, March 01, 2011 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls on immunos If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Tue Mar 1 13:04:17 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Mar 1 13:04:21 2011 Subject: [Histonet] RE: negative controls on immunos In-Reply-To: References: Message-ID: I was also taught to run a positive and negative every time you do an immuno stain, provided there is sufficient tissue. I was trained at AFIP, by the people that wrote the second AFIP staining manual (the red one). Of course, if cost is your number one concern..... Sincerely, Jay A. Lundgren M.S., HTL (ASCP) From mward <@t> wfubmc.edu Tue Mar 1 13:14:15 2011 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Mar 1 13:14:46 2011 Subject: [Histonet] RE: negative controls on immunos In-Reply-To: References: Message-ID: We do not run a second negative control. I agree that it wastes reagents and precious tissue. Martha Ward Wake Forest University Baptist Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Tuesday, March 01, 2011 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls on immunos If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Tue Mar 1 13:35:24 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Mar 1 13:35:34 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per hour? In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7404@EXCHMBC2.ad.ah.local> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7404@EXCHMBC2.ad.ah.local> Message-ID: 30 blocks/hour, including rough cutting, is the standard. That's supposing a few (5-10) levels, specials, etc. If I am cutting all big tissue, what I've heard called "onesies" (just one slide each), I can cut 60 blocks/hour. If one is cutting a tray full of persnickety prostate (with 6 levels on one slide), renal, and bone marrow bxs with tons of specials and immunos, well, that is going to slow you down. Anyway, I disagree with those who say speed isn't as important as quality, patience, etc. I think new histotechs should have someone with a stopwatch and a clipboard standing behind them while they're cutting, yelling "Faster, faster!!!!" in their ear. If anyone cannot measure up to a strict quota they should be immediately dismissed. We have such a disgusting oversupply of new Histotechnology students as it is. ;) Jay A. Lundgren M.S., HTL(ASCP) From PAMarcum <@t> uams.edu Tue Mar 1 14:00:21 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Mar 1 13:58:41 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per hour? In-Reply-To: References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7404@EXCHMBC2.ad.ah.local> Message-ID: I truly hope that was humor about oversupply. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, March 01, 2011 1:35 PM To: Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per hour? 30 blocks/hour, including rough cutting, is the standard. That's supposing a few (5-10) levels, specials, etc. If I am cutting all big tissue, what I've heard called "onesies" (just one slide each), I can cut 60 blocks/hour. If one is cutting a tray full of persnickety prostate (with 6 levels on one slide), renal, and bone marrow bxs with tons of specials and immunos, well, that is going to slow you down. Anyway, I disagree with those who say speed isn't as important as quality, patience, etc. I think new histotechs should have someone with a stopwatch and a clipboard standing behind them while they're cutting, yelling "Faster, faster!!!!" in their ear. If anyone cannot measure up to a strict quota they should be immediately dismissed. We have such a disgusting oversupply of new Histotechnology students as it is. ;) Jay A. Lundgren M.S., HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From lblazek <@t> digestivespecialists.com Tue Mar 1 14:00:45 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Mar 1 14:00:49 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per hour? In-Reply-To: References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7404@EXCHMBC2.ad.ah.local> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EB7EE0263@IBMB7Exchange.digestivespecialists.com> I hope the whole paragraph was supposed to be humor or Jay may have a lynch mob at his door! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 01, 2011 3:00 PM To: 'Jay Lundgren'; Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per hour? I truly hope that was humor about oversupply. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, March 01, 2011 1:35 PM To: Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per hour? 30 blocks/hour, including rough cutting, is the standard. That's supposing a few (5-10) levels, specials, etc. If I am cutting all big tissue, what I've heard called "onesies" (just one slide each), I can cut 60 blocks/hour. If one is cutting a tray full of persnickety prostate (with 6 levels on one slide), renal, and bone marrow bxs with tons of specials and immunos, well, that is going to slow you down. Anyway, I disagree with those who say speed isn't as important as quality, patience, etc. I think new histotechs should have someone with a stopwatch and a clipboard standing behind them while they're cutting, yelling "Faster, faster!!!!" in their ear. If anyone cannot measure up to a strict quota they should be immediately dismissed. We have such a disgusting oversupply of new Histotechnology students as it is. ;) Jay A. Lundgren M.S., HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Tue Mar 1 14:01:42 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Mar 1 14:01:47 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per hour? In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EB7EE0263@IBMB7Exchange.digestivespecialists.com> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7404@EXCHMBC2.ad.ah.local> <5A2BD13465E061429D6455C8D6B40E390EB7EE0263@IBMB7Exchange.digestivespecialists.com> Message-ID: <9BF995BC0E47744E9673A41486E24EE22DBB618E4A@MERCERMAIL.MercerU.local> Bet you he already does. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, March 01, 2011 3:01 PM To: 'Marcum, Pamela A'; 'Jay Lundgren'; Mahoney, Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per hour? I hope the whole paragraph was supposed to be humor or Jay may have a lynch mob at his door! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 01, 2011 3:00 PM To: 'Jay Lundgren'; Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per hour? I truly hope that was humor about oversupply. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, March 01, 2011 1:35 PM To: Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per hour? 30 blocks/hour, including rough cutting, is the standard. That's supposing a few (5-10) levels, specials, etc. If I am cutting all big tissue, what I've heard called "onesies" (just one slide each), I can cut 60 blocks/hour. If one is cutting a tray full of persnickety prostate (with 6 levels on one slide), renal, and bone marrow bxs with tons of specials and immunos, well, that is going to slow you down. Anyway, I disagree with those who say speed isn't as important as quality, patience, etc. I think new histotechs should have someone with a stopwatch and a clipboard standing behind them while they're cutting, yelling "Faster, faster!!!!" in their ear. If anyone cannot measure up to a strict quota they should be immediately dismissed. We have such a disgusting oversupply of new Histotechnology students as it is. ;) Jay A. Lundgren M.S., HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Tue Mar 1 14:10:10 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Mar 1 14:10:17 2011 Subject: [Histonet] HELP!!!!! H. Pylori Immunos In-Reply-To: References: <762291.55285.qm@web80007.mail.sp1.yahoo.com> Message-ID: Our problem, in my opinion is a control issue and not a staining one..our pathologist wants to see the bugs in certain areas-glands(spelling appologies!) its called, the circular groups of cells, in the middle of these areas and not just occasional bugs in other areas....Everywhere else I've worked the pathologist would use a Diff-Quick, Cresyl Violet or Geimsa in addition to the H&E, I'm aware of other newer special stains for H.Pylori that are out there, but this pathologist is firm on the IHC method of choice. I use NovaCastra polyclonal, incubation @23 min. HIER with low PH for 30 minutes.with recommended detection kit.. Heather, I hope you don't mind that I copied your email so that everyone on this forum can learn from your question. I always use the "Reply to All" button so everyone can see my response. I was willing to bet before I read your query that we were dealing with a control problem, not a staining problem. I have seen some miserable commercial *H. pylori* controls lately. Some areas of the country are awash in HP, some labs can't get a good positive control block of their own. You must first obtain a KNOWN POSITIVE CONTROL block. Stain slides from that block with whichever chemical special stain is acceptable to your Pathologist, I like a modified Warthin-Starry. Sit down with your Pathologist and evaluate the control slides. Is it acceptable to the Pathologist? If so, stain more slides from the positive control block, this time immuno stain and chemical special stain in parallel. Evaluate the slides with the Pathologist. Is there correlation between the chemical and special stain? If so, proceed, running your first 20 cases in parallel (immuno and chemical special stains) and documenting (with the Pathologist's signature) the correlation of your first 20 cases. If you are able to demonstrate HP with a chemical special stain, but not with your immuno procedure, call your immuno vendor. If you are unable to demonstrate HP with a chemical special stain, it's your control. Or it could be your stain, but a Giemsa is pretty hard to mess up. Good Luck, Jay A. Lundgren M.S., HTL (ASCP) From jaylundgren <@t> gmail.com Tue Mar 1 14:13:01 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Mar 1 14:13:04 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per hour? In-Reply-To: <9BF995BC0E47744E9673A41486E24EE22DBB618E4A@MERCERMAIL.MercerU.local> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7404@EXCHMBC2.ad.ah.local> <5A2BD13465E061429D6455C8D6B40E390EB7EE0263@IBMB7Exchange.digestivespecialists.com> <9BF995BC0E47744E9673A41486E24EE22DBB618E4A@MERCERMAIL.MercerU.local> Message-ID: ;) From JWeems <@t> sjha.org Tue Mar 1 14:20:46 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Mar 1 14:20:52 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per hour? In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EB7EE0263@IBMB7Exchange.digestivespecialists.com> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7404@EXCHMBC2.ad.ah.local> <5A2BD13465E061429D6455C8D6B40E390EB7EE0263@IBMB7Exchange.digestivespecialists.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081E1BD66F@CHEXCMS10.one.ads.che.org> Humor on Histonet... nevah - as my youngest grandson says about everything....:>) Let's have more.. Report on GMA this morning said humor and good attitude makes us healthier! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From histotech411 <@t> gmail.com Tue Mar 1 15:44:57 2011 From: histotech411 <@t> gmail.com (Jenny Vega) Date: Tue Mar 1 15:45:03 2011 Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? In-Reply-To: References: Message-ID: Hello everybody. Thanks for your replies. I did not expected so many answers to my questions. Well today I talked to my supervisor about ways I could improve the speed of my sectioning, and she and another co-worker pointed out a couple of things that I have been doing that were wasting a lot of time. To me they were "little things" but I stopped doing them today and I was able to finish half- an hour earlier than I am used to. I still need to improve the speed and cut more blocs per hour. I was able to cut about 20-25 an hour but I want to be able to cut more (30 and more)without sacrificing the quality of the blocks. If I don't see the complete tissue on the slide I go crazy. We'll take care. Thanks. On Mon, Feb 28, 2011 at 8:58 PM, Sheila Adey wrote: > Hi Jenny, > This is so dependant on what type of tissue you are cutting and if you are > picking up extra levels or spares. > Also using an automated microtome can be much faster. > Good luck to you. > > > Date: Mon, 28 Feb 2011 18:18:37 -0400 > > From: histotech411@gmail.com > > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] How many tissues an histotech is suppose to cut per > hour? > > > > > I would like to know how many tissues does a experienced histotech is > > suppose to cut per hour (routine h&e slides) in the microtome. I am a new > > histotech and I would like to know how much tissue do an experienced > > histotech cuts per hour. For example if you have 100 tissues per total in > > how many hours are you suppose to cut this tissue? or.....tell me how > much > > you cut at your lab. > > > > Do you have any suggestions on how to cut faster? Thank You. > > > > Thanks > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rbutler <@t> ameripath.com Tue Mar 1 16:53:49 2011 From: rbutler <@t> ameripath.com (Butler, Roszetta) Date: Tue Mar 1 16:54:01 2011 Subject: [Histonet] RE: IF controls In-Reply-To: References: Message-ID: Tonsil controls for C3,C1q,IgA,IgG,IgM,Kappa,Lambda, and Fibrinogen. Used placenta to verify Fibrinogen also. Roszetta Butler, HT(ASCP),IF tech Ameripath, 3000 United Founders Blvd, Suite 135, Oklahoma City, OK 73112, phone (405)841-7875, fax (405)841-2002, email rbutler@ameripath.com "Please think about resource conservation before you print this message CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, March 01, 2011 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 88, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: How many tissues an histotech is suppose to cut per hour? (Podawiltz, Thomas) 2. RE: How many tissues an histo tech is suppose to cut per hour? (Mahoney,Janice A) 3. RE: How many tissues an histotech is suppose to cut per hour? (Marcum, Pamela A) 4. RE: How many tissues an histotech is suppose to cut per hour? (Mahoney,Janice A) 5. looking for KJ Kellogg (Cheri Miller) 6. Slide filing system search (Bartlett, Jeanine (CDC/OID/NCEZID)) 7. Labelers - Needs and Must Haves (Tonia Richmond) 8. RE: Labelers - Needs and Must Haves (Laurie Colbert) 9. Re: How many tissues an histotech is suppose to cut per hour? (Rene J Buesa) 10. I.F. Controls (hardwired@techie.com) 11. RE: Labelers - Needs and Must Haves (WILLIAM DESALVO) 12. HELP!!!!! H. Pylori Immunos (Heather Cooper) ---------------------------------------------------------------------- Message: 1 Date: Tue, 1 Mar 2011 09:26:25 -0500 From: "Podawiltz, Thomas" Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? To: "'Konop, Nicole'" , "histonet@lists.utsouthwestern.edu" , 'Jenny Vega' Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DE133D95@LRGHEXVS1.practice.lrgh.org> Content-Type: text/plain; charset="us-ascii" Worry more about quality than speed at this time. I measure my staff on quality rather than how fast things get done. It's a waste of time, energy and resources to have to re-cut blocks because of poor quality. Speed comes with time and experience. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, March 01, 2011 9:14 AM To: histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Cynthia. Practice and setting up a standard system of how you cut. I do the same thing every time with each block I cut. Cutting faster is not necessarily a good thing if you are jeopardizing quality just to cut more slides. It's quality over quantity in my book any day! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, March 01, 2011 7:51 AM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Practice, practice, practice! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine h&e slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.....tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ------------------------------ Message: 2 Date: Tue, 1 Mar 2011 08:33:19 -0600 From: "Mahoney,Janice A" Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per hour? To: 'Jenny Vega' , "histonet@lists.utsouthwestern.edu" Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7404@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="us-ascii" Hello Jenny, An experienced tech should be able to cut and average of 1 block every 2.5 minutes. Embedding is 1 block per minute ave. Note this is an average. It includes difficult and easy tissue, cleaning the waterbath after each block, writing/printing the slide number, etc. There may be variations in each lab that can change these numbers. Hope this helps. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 4:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine h&e slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.....tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ------------------------------ Message: 3 Date: Tue, 1 Mar 2011 08:58:20 -0600 From: "Marcum, Pamela A" Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? To: "Podawiltz, Thomas" , "'Konop, Nicole'" , "histonet@lists.utsouthwestern.edu" , 'Jenny Vega' Message-ID: Content-Type: text/plain; charset="us-ascii" I agree with Tom. Janice is correct on her timing numbers. However; when you are starting out it is best to get the flow for working at your station than beating a clock. You will gain your speed with learning accuracy which is more important for the patient at the end. We are in patient care not NASCAR and should always remember patient first, which translates to accuracy and the best sections we can produce on every slide. Speed will come as you do more blocks and get better. Pam Marcum ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas [tpodawiltz@lrgh.org] Sent: Tuesday, March 01, 2011 8:26 AM To: 'Konop, Nicole'; histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Worry more about quality than speed at this time. I measure my staff on quality rather than how fast things get done. It's a waste of time, energy and resources to have to re-cut blocks because of poor quality. Speed comes with time and experience. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, March 01, 2011 9:14 AM To: histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Cynthia. Practice and setting up a standard system of how you cut. I do the same thing every time with each block I cut. Cutting faster is not necessarily a good thing if you are jeopardizing quality just to cut more slides. It's quality over quantity in my book any day! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, March 01, 2011 7:51 AM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Practice, practice, practice! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine h&e slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.....tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ------------------------------ Message: 4 Date: Tue, 1 Mar 2011 09:02:17 -0600 From: "Mahoney,Janice A" Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? To: "'Marcum, Pamela A'" , "Podawiltz, Thomas" , "'Konop, Nicole'" , "histonet@lists.utsouthwestern.edu" , 'Jenny Vega' Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7406@EXCHMBC2.ad.ah.local> Content-Type: text/plain; charset="us-ascii" Well said Pam. All the speed in the world means nothing if you can't produce quality slides. The suggestion to do things the same way every time is a good one. However, learning what works well on what tissue is very helpful too. Like icing something that wrinkles or soaking something that shatters, etc. It all comes from experience. I plan to retire in 2 months but will keep my foot in the door working casual so that I don't lose my skills. I love this profession! Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 01, 2011 8:58 AM To: Podawiltz, Thomas; 'Konop, Nicole'; histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Tom. Janice is correct on her timing numbers. However; when you are starting out it is best to get the flow for working at your station than beating a clock. You will gain your speed with learning accuracy which is more important for the patient at the end. We are in patient care not NASCAR and should always remember patient first, which translates to accuracy and the best sections we can produce on every slide. Speed will come as you do more blocks and get better. Pam Marcum ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas [tpodawiltz@lrgh.org] Sent: Tuesday, March 01, 2011 8:26 AM To: 'Konop, Nicole'; histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Worry more about quality than speed at this time. I measure my staff on quality rather than how fast things get done. It's a waste of time, energy and resources to have to re-cut blocks because of poor quality. Speed comes with time and experience. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, March 01, 2011 9:14 AM To: histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Cynthia. Practice and setting up a standard system of how you cut. I do the same thing every time with each block I cut. Cutting faster is not necessarily a good thing if you are jeopardizing quality just to cut more slides. It's quality over quantity in my book any day! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, March 01, 2011 7:51 AM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Practice, practice, practice! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine h&e slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.....tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ------------------------------ Message: 5 Date: Tue, 1 Mar 2011 09:12:45 -0600 From: Cheri Miller Subject: [Histonet] looking for KJ Kellogg To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I am looking for KJ Kellogg, or Kjirsten Kellogg she is a HT/PA ASCP working some where in Kansas I think. KJ is originally from Wisconsin. I have an urgent, personal message for her. Thanks, Cheri Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor, Safety Officer, Physicians Laboratory Services Omaha, NE. 68127 402 731 4145 ext. 554 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 6 Date: Tue, 1 Mar 2011 16:29:07 +0000 From: "Bartlett, Jeanine (CDC/OID/NCEZID)" Subject: [Histonet] Slide filing system search To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, We are looking for a system of some sort that will allow us to group interesting cases together for quick review. We will use this for teaching, visiting scientists, etc. We want something that will allow for the slides to be stored flat and not in a vertical position, if at all possible. Anyone know of anything? We are talking about quite a few cases...maybe 100+. Vendors welcome to reply. Thanks in advance! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov ------------------------------ Message: 7 Date: Tue, 1 Mar 2011 16:35:22 +0000 (GMT) From: Tonia Richmond Subject: [Histonet] Labelers - Needs and Must Haves To: histonet@lists.utsouthwestern.edu Message-ID: <110122392.12944.1298997322264.JavaMail.mail@webmail20> Content-Type: text/plain; charset="UTF-8" If you had the chance to pick features that are a MUST HAVE for cassette and slide labelers what would those features/must haves be? Curious to Know Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ------------------------------ Message: 8 Date: Tue, 1 Mar 2011 08:50:01 -0800 From: "Laurie Colbert" Subject: RE: [Histonet] Labelers - Needs and Must Haves To: "Tonia Richmond" , Message-ID: <57BE698966D5C54EAE8612E8941D768301269027@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="UTF-8" The ability to print barcodes and read barcodes -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tonia Richmond Sent: Tuesday, March 01, 2011 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Labelers - Needs and Must Haves If you had the chance to pick features that are a MUST HAVE for cassette and slide labelers what would those features/must haves be? Curious to Know Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 1 Mar 2011 08:51:01 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] How many tissues an histotech is suppose to cut per hour? To: histonet@lists.utsouthwestern.edu, Jenny Vega Message-ID: <244978.10684.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 To cut 24 single slides blcoks/hour Ren? J. --- On Mon, 2/28/11, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? To: histonet@lists.utsouthwestern.edu Date: Monday, February 28, 2011, 5:18 PM I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine h&e slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.....tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 01 Mar 2011 12:01:58 -0500 From: hardwired@techie.com Subject: [Histonet] I.F. Controls To: histonet@lists.utsouthwestern.edu Message-ID: <8CDA649826C080C-1124-905E@web-mmc-m05.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hi everyone, Just wondering what you all use as controls for I.F. kindney biopsies!! Thanks D ------------------------------ Message: 11 Date: Tue, 1 Mar 2011 10:03:16 -0700 From: WILLIAM DESALVO Subject: RE: [Histonet] Labelers - Needs and Must Haves To: , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" We just went through an evaluation for cassette printers that produce a 2D bar code. Top features we looked for: Print Accession#, last name/first initial, and 2D bar code under 10 seconds a cassette Minimal jamming/defects of printed cassettes, 1 defect/100 cassette or less Tubed or taped cassettes for hoppers for quick and easy loading Cost of cassettes available for use time and complexity of daily, weekly, monthly maintenance of instrument Ease of use by employees Interfaced to multiple LIS Compatible with work flow software and vendor supported We found that the cassette printers fell into three are types of printers available, tape/ribbon transfer, ink jet and laser etching. All three types have there pluses and minuses. William DeSalvo, B.S., HTL(ASCP) > Date: Tue, 1 Mar 2011 16:35:22 +0000 > From: tjrichmond@hughes.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Labelers - Needs and Must Haves > > > If you had the chance to pick features that are a MUST HAVE for > cassette and slide labelers what would those features/must haves be? > Curious to Know > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message.. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 1 Mar 2011 09:50:40 -0800 (PST) From: Heather Cooper Subject: [Histonet] HELP!!!!! H. Pylori Immunos To: histonet@lists.utsouthwestern.edu Message-ID: <762291.55285.qm@web80007.mail.sp1.yahoo.com> Content-Type: text/plain; charset=us-ascii Does anyone do H. Pylori Immunos by hand? ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 88, Issue 2 *************************************** From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Mar 1 18:24:04 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Mar 1 18:26:03 2011 Subject: [Histonet] HELP!!!!! H. Pylori Immunos In-Reply-To: References: <762291.55285.qm@web80007.mail.sp1.yahoo.com> , Message-ID: We also cut our controls in sequence. Stain the first of the sequence and the last. To make sure that you have not cut through the area. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________ From: Jay Lundgren [jaylundgren@gmail.com] Sent: Tuesday, March 01, 2011 3:10 PM To: McMahon, Loralee A Cc: Heather Cooper; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HELP!!!!! H. Pylori Immunos Our problem, in my opinion is a control issue and not a staining one..our pathologist wants to see the bugs in certain areas-glands(spelling appologies!) its called, the circular groups of cells, in the middle of these areas and not just occasional bugs in other areas....Everywhere else I've worked the pathologist would use a Diff-Quick, Cresyl Violet or Geimsa in addition to the H&E, I'm aware of other newer special stains for H.Pylori that are out there, but this pathologist is firm on the IHC method of choice. I use NovaCastra polyclonal, incubation @23 min. HIER with low PH for 30 minutes.with recommended detection kit.. Heather, I hope you don't mind that I copied your email so that everyone on this forum can learn from your question. I always use the "Reply to All" button so everyone can see my response. I was willing to bet before I read your query that we were dealing with a control problem, not a staining problem. I have seen some miserable commercial H. pylori controls lately. Some areas of the country are awash in HP, some labs can't get a good positive control block of their own. You must first obtain a KNOWN POSITIVE CONTROL block. Stain slides from that block with whichever chemical special stain is acceptable to your Pathologist, I like a modified Warthin-Starry. Sit down with your Pathologist and evaluate the control slides. Is it acceptable to the Pathologist? If so, stain more slides from the positive control block, this time immuno stain and chemical special stain in parallel. Evaluate the slides with the Pathologist. Is there correlation between the chemical and special stain? If so, proceed, running your first 20 cases in parallel (immuno and chemical special stains) and documenting (with the Pathologist's signature) the correlation of your first 20 cases. If you are able to demonstrate HP with a chemical special stain, but not with your immuno procedure, call your immuno vendor. If you are unable to demonstrate HP with a chemical special stain, it's your control. Or it could be your stain, but a Giemsa is pretty hard to mess up. Good Luck, Jay A. Lundgren M.S., HTL (ASCP) From JMacDonald <@t> mtsac.edu Tue Mar 1 23:37:40 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Mar 1 23:37:45 2011 Subject: [Histonet] Traveling Histotechs Message-ID: Does anyone have information on any agencies that specialize in positions for traveling histotechs? Thank you, Jennifer MacDonald From bakevictoria <@t> gmail.com Wed Mar 2 02:27:37 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Wed Mar 2 02:27:58 2011 Subject: [Histonet] Traveling Histotechs In-Reply-To: References: Message-ID: Aureus Medical Staffing - Omaha NE Club Staffing - FL TravelMax - not sure where they are located Ampian - Nevada Full Staff - TX CompHealth - not sure which site does travel work at this point These are just a few of the many I'm sure. Vikki On Wed, Mar 2, 2011 at 12:37 AM, Jennifer MacDonald wrote: > Does anyone have information on any agencies that specialize in positions > for traveling histotechs? > Thank you, > Jennifer MacDonald > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mab70 <@t> medschl.cam.ac.uk Wed Mar 2 04:54:19 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Wed Mar 2 04:54:26 2011 Subject: [Histonet] Fat tissue Message-ID: Has anyone experience of cryostat sectioning of fat tissue? This would be rodent fat pads, particularly the gonadal fat pad which usually consists largely of adipocytes. What temperature would you cut at? Is there anything that could be used to infiltrate the tissue prior to sectioning to provide more support? Regards Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 From rsrichmond <@t> gmail.com Wed Mar 2 07:33:25 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Mar 2 07:33:34 2011 Subject: [Histonet] Re: Sentinel Lymph Node Procedure Message-ID: Allison Scott HT(ASCP), Histology Supervisor at LBJ Hospital in Houston, Texas asks about sentinel lymph node procedures. About a month ago an article appeared in the New England Journal of Medicine suggesting that a single section of a lymph node block, without immunostains, was sufficient for detecting clinically significant metastases from breast cancers. (Note that the article says nothing about melanoma.) "Effect of Occult Metastases on Survival in Node-Negative Breast Cancer" Donald L. Weaver, M.D., et al. N Engl J Med 2011; 364:412-421February 3, 2011 I can send you a PDF of this article if you'll e-mail me privately. Bob Richmond Samurai Pathologist Knoxville TN rsrichmond@gmail.com From gvdobbin <@t> ihis.org Wed Mar 2 08:15:07 2011 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Wed Mar 2 08:15:45 2011 Subject: [Histonet] negative controls on immunos In-Reply-To: References: Message-ID: <4D6E18AB020000C80000F33E@smtp1.gov.pe.ca> Yes, it is always best to run every conceivable control available. Then you can be really, really, really sure. However, if you have practical issues that come into play such as cost of reagents, or extra controls taking up valuable space on the stainer then you might have to think about what you are trying to accomplish with each control and balance the cost vs benefit. So what are we checking with the negative control? We are checking the reagents to ensure that there is no non-specific reaction arising from the detection kit or the buffers, etc. If you are running the same block tomorrow with the same detection kit (ie same lot) it is not necessary (in my humble opinion) to check it again with another negative control. I suppose if you are worried that someone could be trying to sabotage your work by sneaking into your lab at night and contaminating your detection kit...then the simplest way to detect this sinister activity would be to run a negative control every time. Otherwise, I think one negative control slide per block per detection kit will be adequate. Disclaimer: Please know that I have just tried to inject a little humor into this response. I am really not trying to be sarcastic in anyway. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Diana McCaig" 3/1/2011 2:07 PM >>> If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From sgoebel <@t> mirnarx.com Wed Mar 2 09:01:31 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Mar 2 09:01:34 2011 Subject: [Histonet] Fat tissue In-Reply-To: References: Message-ID: For the most part you cannot section fat on a cryostat because of the oilyness (is that a word). You can fix it and section it? Good Luck Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaret Blount Sent: Wednesday, March 02, 2011 4:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fat tissue Has anyone experience of cryostat sectioning of fat tissue? This would be rodent fat pads, particularly the gonadal fat pad which usually consists largely of adipocytes. What temperature would you cut at? Is there anything that could be used to infiltrate the tissue prior to sectioning to provide more support? Regards Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Mar 2 09:30:02 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Mar 2 09:30:08 2011 Subject: [Histonet] negative controls on immunos In-Reply-To: <4D6E18AB020000C80000F33E@smtp1.gov.pe.ca> References: <4D6E18AB020000C80000F33E@smtp1.gov.pe.ca> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081E1BD7BC@CHEXCMS10.one.ads.che.org> The CAP guidelines are pretty clear. Copied from latest checklist.. Isn't this fun???????????!!!!!!!!! j:>) ANP.22570 Phase II N/A YES NO Are appropriate negative controls used? NOTE: Negative controls must assess the presence of nonspecific staining in patient tissue as well as the specificity of each antibody. Results of controls must be documented, either in internal laboratory records, or in the patient report. A statement in the report such as, "All controls show appropriate reactivity" is sufficient. A negative reagent control is used to assess nonspecific or aberrant staining in patient tissue related to the antigen retrieval conditions and/or detection system used. A separate section of patient tissue is processed using the same reagent and epitope retrieval protocol as the patient test slide, except that the primary antibody is omitted, and replaced by any one of the following: ? An unrelated antibody of the same isotype as the primary antibody (for monoclonal primary antibodies) ? An unrelated antibody from the same animal species as the primary antibody (for polyclonal primary antibodies) ? The negative control reagent included in the staining kit ? The diluent/buffer solution in which the primary antibody is diluted In general, a separate negative reagent control should be run for each block of patient tissue being immunostained; however, for cases in which there is simultaneous staining of multiple blocks from the same specimen with the same antibody (e.g., cytokeratin staining of multiple axillary sentinel lymph nodes), performing a single negative control on one of the blocks may be sufficient provided that all such blocks are fixed and processed identically. This exception does not apply to stains on different types of tissues or those using different antigen retrieval protocols or antibody detection systems. The laboratory director must determine which cases will have only one negative reagent control, and this must be specified in the department's procedure manual. The negative reagent control would ideally control for each reagent protocol and antibody retrieval condition; however, large antibody panels often employ multiple antigen retrieval procedures. In such cases, a reasonable minimum control would be to perform the negative reagent control using the most aggressive retrieval procedure in the particular antibody panel. Aggressiveness of antigen retrieval (in decreasing order) is as follows: pressure cooker; enzyme digestion, boiling; microwave; steamer; water bath. High pH retrieval should be considered more aggressive than comparable retrieval in citrate buffer at pH 6.0. It is also important to assess the specificity of each antibody by a negative tissue control, which must show no staining of tissues known to lack the antigen. The negative tissue control is processed using the same fixation, epitope retrieval and immunostaining protocols as the patient tissue. Unexpected positive staining of such tissues indicates that the test has lost specificity, perhaps because of improper antibody concentration or excessive antigen retrieval. Intrinsic properties of the test tissue may also be the cause of "non-specific" staining. For example, tissues with high endogenous biotin activity such as liver or renal tubules may simulate positive staining when using a detection method based on biotin labeling. A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks. These can provide simultaneous positive and negative tissue controls, and are considered "best practice" (see below). 2. The positive control slide or patient test slides, if these slides contain tissue elements that should not react with the antibody. 3. A separate negative tissue control slide. The type of negative tissue control used (i.e., separate sections, internal controls or multitissue blocks) should be specified in the laboratory manual (refer to ANP.22250). Multitissue blocks may be considered best practice and can have a major role in maintaining quality. When used as a combined positive and negative tissue control as mentioned above, they can serve as a permanent record documenting the sensitivity and specificity of every stain, particularly when mounted on the same slide as the patient tissue. When the components are chosen appropriately, multitissue blocks may be used for many different primary antibodies, decreasing the number of different control blocks needed by the laboratory. Multitissue blocks are also ideal for determining optimal titers of primary antibodies since they allow simultaneous evaluation of many different pieces of tissue. Finally, they are a useful and efficient means to screen new antibodies for sensitivity and specificity or new lots of antibody for consistency, which should be done before putting any antibody into diagnostic use. REFERENCES 1) Leong AS-Y, Cooper K, Leong FJW-M. Manual of Diagnostic Antibodies for Immunohistology. 2nd ed. London: Greenwich Medical Media; 2003 2) Dabbs DJ. Diagnostic Immunohistochemistry. New York: Churchill Livingstone; 2002 3) Burry RW. Specificity controls for immunocytochemical methods. J Histochem Cytochem 2000;48:163-166 Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Wednesday, March 02, 2011 09:15 To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] negative controls on immunos Yes, it is always best to run every conceivable control available. Then you can be really, really, really sure. However, if you have practical issues that come into play such as cost of reagents, or extra controls taking up valuable space on the stainer then you might have to think about what you are trying to accomplish with each control and balance the cost vs benefit. So what are we checking with the negative control? We are checking the reagents to ensure that there is no non-specific reaction arising from the detection kit or the buffers, etc. If you are running the same block tomorrow with the same detection kit (ie same lot) it is not necessary (in my humble opinion) to check it again with another negative control. I suppose if you are worried that someone could be trying to sabotage your work by sneaking into your lab at night and contaminating your detection kit...then the simplest way to detect this sinister activity would be to run a negative control every time. Otherwise, I think one negative control slide per block per detection kit will be adequate. Disclaimer: Please know that I have just tried to inject a little humor into this response. I am really not trying to be sarcastic in anyway. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Diana McCaig" 3/1/2011 2:07 PM >>> If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From gvdobbin <@t> ihis.org Wed Mar 2 09:39:16 2011 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Wed Mar 2 09:39:55 2011 Subject: [Histonet] negative controls on immunos In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164081E1BD7BC@CHEXCMS10.one.ads.che.org> References: <4D6E18AB020000C80000F33E@smtp1.gov.pe.ca> <92AD9B20A6C38C4587A9FEBE3A30E164081E1BD7BC@CHEXCMS10.one.ads.che.org> Message-ID: <4D6E2C64020000C80000F360@smtp1.gov.pe.ca> Thanks Joyce. This excerpt supports what I had said- noting the difference between the negative (or deletion) control (which is what I was addressing in my reply) and the negative tissue control. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Weems, Joyce" 3/2/2011 11:30 AM >>> The CAP guidelines are pretty clear. Copied from latest checklist.. Isn't this fun???????????!!!!!!!!! j:>) ANP.22570 Phase II N/A YES NO Are appropriate negative controls used? NOTE: Negative controls must assess the presence of nonspecific staining in patient tissue as well as the specificity of each antibody. Results of controls must be documented, either in internal laboratory records, or in the patient report. A statement in the report such as, "All controls show appropriate reactivity" is sufficient. A negative reagent control is used to assess nonspecific or aberrant staining in patient tissue related to the antigen retrieval conditions and/or detection system used. A separate section of patient tissue is processed using the same reagent and epitope retrieval protocol as the patient test slide, except that the primary antibody is omitted, and replaced by any one of the following: ? An unrelated antibody of the same isotype as the primary antibody (for monoclonal primary antibodies) ? An unrelated antibody from the same animal species as the primary antibody (for polyclonal primary antibodies) ? The negative control reagent included in the staining kit ? The diluent/buffer solution in which the primary antibody is diluted In general, a separate negative reagent control should be run for each block of patient tissue being immunostained; however, for cases in which there is simultaneous staining of multiple blocks from the same specimen with the same antibody (e.g., cytokeratin staining of multiple axillary sentinel lymph nodes), performing a single negative control on one of the blocks may be sufficient provided that all such blocks are fixed and processed identically. This exception does not apply to stains on different types of tissues or those using different antigen retrieval protocols or antibody detection systems. The laboratory director must determine which cases will have only one negative reagent control, and this must be specified in the department's procedure manual. The negative reagent control would ideally control for each reagent protocol and antibody retrieval condition; however, large antibody panels often employ multiple antigen retrieval procedures. In such cases, a reasonable minimum control would be to perform the negative reagent control using the most aggressive retrieval procedure in the particular antibody panel. Aggressiveness of antigen retrieval (in decreasing order) is as follows: pressure cooker; enzyme digestion, boiling; microwave; steamer; water bath. High pH retrieval should be considered more aggressive than comparable retrieval in citrate buffer at pH 6.0. It is also important to assess the specificity of each antibody by a negative tissue control, which must show no staining of tissues known to lack the antigen. The negative tissue control is processed using the same fixation, epitope retrieval and immunostaining protocols as the patient tissue. Unexpected positive staining of such tissues indicates that the test has lost specificity, perhaps because of improper antibody concentration or excessive antigen retrieval. Intrinsic properties of the test tissue may also be the cause of "non-specific" staining. For example, tissues with high endogenous biotin activity such as liver or renal tubules may simulate positive staining when using a detection method based on biotin labeling. A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks. These can provide simultaneous positive and negative tissue controls, and are considered "best practice" (see below). 2. The positive control slide or patient test slides, if these slides contain tissue elements that should not react with the antibody. 3. A separate negative tissue control slide. The type of negative tissue control used (i.e., separate sections, internal controls or multitissue blocks) should be specified in the laboratory manual (refer to ANP.22250). Multitissue blocks may be considered best practice and can have a major role in maintaining quality. When used as a combined positive and negative tissue control as mentioned above, they can serve as a permanent record documenting the sensitivity and specificity of every stain, particularly when mounted on the same slide as the patient tissue. When the components are chosen appropriately, multitissue blocks may be used for many different primary antibodies, decreasing the number of different control blocks needed by the laboratory. Multitissue blocks are also ideal for determining optimal titers of primary antibodies since they allow simultaneous evaluation of many different pieces of tissue. Finally, they are a useful and efficient means to screen new antibodies for sensitivity and specificity or new lots of antibody for consistency, which should be done before putting any antibody into diagnostic use. REFERENCES 1) Leong AS-Y, Cooper K, Leong FJW-M. Manual of Diagnostic Antibodies for Immunohistology. 2nd ed. London: Greenwich Medical Media; 2003 2) Dabbs DJ. Diagnostic Immunohistochemistry. New York: Churchill Livingstone; 2002 3) Burry RW. Specificity controls for immunocytochemical methods. J Histochem Cytochem 2000;48:163-166 Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Wednesday, March 02, 2011 09:15 To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] negative controls on immunos Yes, it is always best to run every conceivable control available. Then you can be really, really, really sure. However, if you have practical issues that come into play such as cost of reagents, or extra controls taking up valuable space on the stainer then you might have to think about what you are trying to accomplish with each control and balance the cost vs benefit. So what are we checking with the negative control? We are checking the reagents to ensure that there is no non-specific reaction arising from the detection kit or the buffers, etc. If you are running the same block tomorrow with the same detection kit (ie same lot) it is not necessary (in my humble opinion) to check it again with another negative control. I suppose if you are worried that someone could be trying to sabotage your work by sneaking into your lab at night and contaminating your detection kit...then the simplest way to detect this sinister activity would be to run a negative control every time. Otherwise, I think one negative control slide per block per detection kit will be adequate. Disclaimer: Please know that I have just tried to inject a little humor into this response. I am really not trying to be sarcastic in anyway. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Diana McCaig" 3/1/2011 2:07 PM >>> If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From DSiena <@t> statlab.com Wed Mar 2 09:43:10 2011 From: DSiena <@t> statlab.com (Debra Siena) Date: Wed Mar 2 09:43:15 2011 Subject: [Histonet] bone marrow aspirations Message-ID: Hi All, I would appreciate some insight as to how most labs are treating bone marrow smears, in regards to fixation. I am not embarrassed to say that it has been awhile since I worked with them. With the smears are most labs air drying, methanol (alcohol fixation) or spray fixation (pap fixative) or something else? I do thank you for your help. Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsiena@statlab.com | www.statlab.com From rjbuesa <@t> yahoo.com Wed Mar 2 10:03:30 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 2 10:03:34 2011 Subject: [Histonet] bone marrow aspirations In-Reply-To: Message-ID: <389796.27072.qm@web65709.mail.ac4.yahoo.com> Either air dry ? methanol (most labs) or air dry ? PAP fixative (I always preferred methanol). Ren? J. --- On Wed, 3/2/11, Debra Siena wrote: From: Debra Siena Subject: [Histonet] bone marrow aspirations To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 2, 2011, 10:43 AM Hi All, I would appreciate some insight as to how most labs are treating bone marrow smears, in regards to fixation.? I am not embarrassed to say that it has been awhile since I worked with them.? With the smears are most labs air drying, methanol (alcohol fixation) or spray fixation (pap fixative) or something else?? I do thank you for your help. Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010? x229 | Fax: 972-436-1369 dsiena@statlab.com | www.statlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Wed Mar 2 10:09:34 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Mar 2 10:09:47 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per hour? In-Reply-To: <9BF995BC0E47744E9673A41486E24EE22DBB618E4A@MERCERMAIL.MercerU.local> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7404@EXCHMBC2.ad.ah.local> <5A2BD13465E061429D6455C8D6B40E390EB7EE0263@IBMB7Exchange.digestivespecialists.com> <9BF995BC0E47744E9673A41486E24EE22DBB618E4A@MERCERMAIL.MercerU.local> Message-ID: <4D6E256E.2B7F.00C9.1@geisinger.edu> you all saw the little wink ;) at the end of his paragraph, right???? >>> "Shirley A. Powell" 3/1/2011 3:01 PM >>> Bet you he already does. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, March 01, 2011 3:01 PM To: 'Marcum, Pamela A'; 'Jay Lundgren'; Mahoney, Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per hour? I hope the whole paragraph was supposed to be humor or Jay may have a lynch mob at his door! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 01, 2011 3:00 PM To: 'Jay Lundgren'; Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per hour? I truly hope that was humor about oversupply. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, March 01, 2011 1:35 PM To: Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per hour? 30 blocks/hour, including rough cutting, is the standard. That's supposing a few (5-10) levels, specials, etc. If I am cutting all big tissue, what I've heard called "onesies" (just one slide each), I can cut 60 blocks/hour. If one is cutting a tray full of persnickety prostate (with 6 levels on one slide), renal, and bone marrow bxs with tons of specials and immunos, well, that is going to slow you down. Anyway, I disagree with those who say speed isn't as important as quality, patience, etc. I think new histotechs should have someone with a stopwatch and a clipboard standing behind them while they're cutting, yelling "Faster, faster!!!!" in their ear. If anyone cannot measure up to a strict quota they should be immediately dismissed. We have such a disgusting oversupply of new Histotechnology students as it is. ;) Jay A. Lundgren M.S., HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From scorpionrider <@t> cox.net Wed Mar 2 10:33:49 2011 From: scorpionrider <@t> cox.net (Mark Turner) Date: Wed Mar 2 10:34:04 2011 Subject: [Histonet] bone marrow aspirations In-Reply-To: <389796.27072.qm@web65709.mail.ac4.yahoo.com> Message-ID: <20110302113349.XK9R1.616018.imail@fed1rmwml44> I've generally used the methanol fixation. Mark Turner ---- Rene J Buesa wrote: > Either air dry ? methanol (most labs) or air dry ? PAP fixative (I always preferred methanol). > Ren? J. > > --- On Wed, 3/2/11, Debra Siena wrote: > > > From: Debra Siena > Subject: [Histonet] bone marrow aspirations > To: "histonet@lists.utsouthwestern.edu" > Date: Wednesday, March 2, 2011, 10:43 AM > > > Hi All, > > I would appreciate some insight as to how most labs are treating bone marrow smears, in regards to fixation.? I am not embarrassed to say that it has been awhile since I worked with them.? With the smears are most labs air drying, methanol (alcohol fixation) or spray fixation (pap fixative) or something else?? I do thank you for your help. > > Debbie Siena HT(ASCP)QIHC > Technical Manager | StatLab Medical Products > 407 Interchange St. | McKinney, TX 75071 > Direct: 972-436-1010? x229 | Fax: 972-436-1369 > dsiena@statlab.com | www.statlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Wed Mar 2 10:42:45 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Mar 2 10:42:59 2011 Subject: [Histonet] negative controls on immunos In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164081E1BD7BC@CHEXCMS10.one.ads.che.org> References: <4D6E18AB020000C80000F33E@smtp1.gov.pe.ca> <92AD9B20A6C38C4587A9FEBE3A30E164081E1BD7BC@CHEXCMS10.one.ads.che.org> Message-ID: <4D6E2D35.2B7F.00C9.1@geisinger.edu> Fun no, impractical yes. >>> "Weems, Joyce" 3/2/2011 10:30 AM >>> The CAP guidelines are pretty clear. Copied from latest checklist.. Isn't this fun???????????!!!!!!!!! j:>) ANP.22570 Phase II N/A YES NO Are appropriate negative controls used? NOTE: Negative controls must assess the presence of nonspecific staining in patient tissue as well as the specificity of each antibody. Results of controls must be documented, either in internal laboratory records, or in the patient report. A statement in the report such as, "All controls show appropriate reactivity" is sufficient. A negative reagent control is used to assess nonspecific or aberrant staining in patient tissue related to the antigen retrieval conditions and/or detection system used. A separate section of patient tissue is processed using the same reagent and epitope retrieval protocol as the patient test slide, except that the primary antibody is omitted, and replaced by any one of the following: ? An unrelated antibody of the same isotype as the primary antibody (for monoclonal primary antibodies) ? An unrelated antibody from the same animal species as the primary antibody (for polyclonal primary antibodies) ? The negative control reagent included in the staining kit ? The diluent/buffer solution in which the primary antibody is diluted In general, a separate negative reagent control should be run for each block of patient tissue being immunostained; however, for cases in which there is simultaneous staining of multiple blocks from the same specimen with the same antibody (e.g., cytokeratin staining of multiple axillary sentinel lymph nodes), performing a single negative control on one of the blocks may be sufficient provided that all such blocks are fixed and processed identically. This exception does not apply to stains on different types of tissues or those using different antigen retrieval protocols or antibody detection systems. The laboratory director must determine which cases will have only one negative reagent control, and this must be specified in the department's procedure manual. The negative reagent control would ideally control for each reagent protocol and antibody retrieval condition; however, large antibody panels often employ multiple antigen retrieval procedures. In such cases, a reasonable minimum control would be to perform the negative reagent control using the most aggressive retrieval procedure in the particular antibody panel. Aggressiveness of antigen retrieval (in decreasing order) is as follows: pressure cooker; enzyme digestion, boiling; microwave; steamer; water bath. High pH retrieval should be considered more aggressive than comparable retrieval in citrate buffer at pH 6.0. It is also important to assess the specificity of each antibody by a negative tissue control, which must show no staining of tissues known to lack the antigen. The negative tissue control is processed using the same fixation, epitope retrieval and immunostaining protocols as the patient tissue. Unexpected positive staining of such tissues indicates that the test has lost specificity, perhaps because of improper antibody concentration or excessive antigen retrieval. Intrinsic properties of the test tissue may also be the cause of "non-specific" staining. For example, tissues with high endogenous biotin activity such as liver or renal tubules may simulate positive staining when using a detection method based on biotin labeling. A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks. These can provide simultaneous positive and negative tissue controls, and are considered "best practice" (see below). 2. The positive control slide or patient test slides, if these slides contain tissue elements that should not react with the antibody. 3. A separate negative tissue control slide. The type of negative tissue control used (i.e., separate sections, internal controls or multitissue blocks) should be specified in the laboratory manual (refer to ANP.22250). Multitissue blocks may be considered best practice and can have a major role in maintaining quality. When used as a combined positive and negative tissue control as mentioned above, they can serve as a permanent record documenting the sensitivity and specificity of every stain, particularly when mounted on the same slide as the patient tissue. When the components are chosen appropriately, multitissue blocks may be used for many different primary antibodies, decreasing the number of different control blocks needed by the laboratory. Multitissue blocks are also ideal for determining optimal titers of primary antibodies since they allow simultaneous evaluation of many different pieces of tissue. Finally, they are a useful and efficient means to screen new antibodies for sensitivity and specificity or new lots of antibody for consistency, which should be done before putting any antibody into diagnostic use. REFERENCES 1) Leong AS-Y, Cooper K, Leong FJW-M. Manual of Diagnostic Antibodies for Immunohistology. 2nd ed. London: Greenwich Medical Media; 2003 2) Dabbs DJ. Diagnostic Immunohistochemistry. New York: Churchill Livingstone; 2002 3) Burry RW. Specificity controls for immunocytochemical methods. J Histochem Cytochem 2000;48:163-166 Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Wednesday, March 02, 2011 09:15 To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] negative controls on immunos Yes, it is always best to run every conceivable control available. Then you can be really, really, really sure. However, if you have practical issues that come into play such as cost of reagents, or extra controls taking up valuable space on the stainer then you might have to think about what you are trying to accomplish with each control and balance the cost vs benefit. So what are we checking with the negative control? We are checking the reagents to ensure that there is no non-specific reaction arising from the detection kit or the buffers, etc. If you are running the same block tomorrow with the same detection kit (ie same lot) it is not necessary (in my humble opinion) to check it again with another negative control. I suppose if you are worried that someone could be trying to sabotage your work by sneaking into your lab at night and contaminating your detection kit...then the simplest way to detect this sinister activity would be to run a negative control every time. Otherwise, I think one negative control slide per block per detection kit will be adequate. Disclaimer: Please know that I have just tried to inject a little humor into this response. I am really not trying to be sarcastic in anyway. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Diana McCaig" 3/1/2011 2:07 PM >>> If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From JMacDonald <@t> mtsac.edu Wed Mar 2 10:47:09 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Mar 2 10:47:17 2011 Subject: [Histonet] bone marrow aspirations In-Reply-To: Message-ID: We always air dried them and fixed them in methanol, otherwise they washed off. Debra Siena Sent by: histonet-bounces@lists.utsouthwestern.edu 03/02/2011 07:49 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] bone marrow aspirations Hi All, I would appreciate some insight as to how most labs are treating bone marrow smears, in regards to fixation. I am not embarrassed to say that it has been awhile since I worked with them. With the smears are most labs air drying, methanol (alcohol fixation) or spray fixation (pap fixative) or something else? I do thank you for your help. Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsiena@statlab.com | www.statlab.com< http://www.statlab.com/> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Mar 2 10:52:40 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Mar 2 10:52:47 2011 Subject: AW: [Histonet] Sentinel Lymp Node Procedure In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFD76@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC026DFD76@LBEXCH01.hchd.local> Message-ID: For breast sentinels: Sentinels are received fresh. Sentinels below 4 mm diameter are cut in two halfes - one for frozens, one for paraffin. Sentinels bigger than 4 mm diameter are cut in 2 mm slices - the middle-slice for frozens, the rest for paraffin. Frozens: 3 sections; 1. and 3. for HE; the middle for Fast-CK-Pan Paraffin: sections each 250 ?m, first and last section for HE, the sections between for CK-Pan immunohisto. Positiv lymphnodes found in frozens -- then only two sections for HE. Gudrun Lang Histolab, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Scott, Allison D Gesendet: Dienstag, 01. M?rz 2011 19:10 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Sentinel Lymp Node Procedure Hello to all in histoland. Does anyone have a procedure for sentinel lymph nodes that they would be willing to share. I am revamoing mine. We are in our CAP window. Looking for them in the next 2 weeks. Any help will be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSiena <@t> statlab.com Wed Mar 2 11:33:19 2011 From: DSiena <@t> statlab.com (Debra Siena) Date: Wed Mar 2 11:33:28 2011 Subject: [Histonet] Bone marrow smears Message-ID: Hi All, It appears that air drying and then methanol fixation is the method most labs are using on bone marrow smears. Thanks for all your help, I do appreciate all the responses. Best Wishes Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsiena@statlab.com | www.statlab.com From Joyce.Fortin <@t> uhsinc.com Wed Mar 2 12:21:14 2011 From: Joyce.Fortin <@t> uhsinc.com (Fortin, Joyce) Date: Wed Mar 2 12:21:21 2011 Subject: [Histonet] Competancies for handling hazardous material In-Reply-To: References: <000601cbd3c0$d7ebaf80$87c30e80$@grics.net>, Message-ID: Could I PLEASE have this information, too? I would really appreciate it. Joyce Fortin Histology Supervisor Palmdale Regional Medical Center 38600 Medical Center Drive Palmdale, California 93551 Phone 661-382-5723 Fax 661-382-5747 email: joyce.fortin@uhsinc.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala [liz@premierlab.com] Sent: Wednesday, February 23, 2011 6:51 PM To: Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material Rae We have, you need standard training, but the individuals who inspected our lab did not mention compentances. But our inspection happened because we needed to switch from a conditionally exempt small quantity generator to a small quantitiy generator and we then needed to register with the state, etc. They primarily focused on our waste streams and what chemicals were in the lab. I'm not at work right now, but I'll check tomorrow to see if I have anything that I can share with you. Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rae Staskiewicz Sent: Wed 2/23/2011 6:19 PM To: Histonet Subject: [Histonet] Competancies for handling hazardous material Has anyone been inspected by the EPA regarding removal of hazardous chemicals? It came up in a safety meeting that I should have training and competencies (beyond general safety training and PPE) for pouring xylene from staining dishes and processors into accumulation containers. Rae Ann Staskiewicz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. From akemiat3377 <@t> yahoo.com Wed Mar 2 12:42:09 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Mar 2 12:42:12 2011 Subject: [Histonet] Alcohol & Xylene recyclers Message-ID: <765336.52766.qm@web113811.mail.gq1.yahoo.com> Hi Everyone in histoland! I would like to get your feedback on which alcohol / xylene recyling units you prefer.? I would like?information regarding?purity of end product, cost, size of footprint, and relyability.? We currently do not have a recycling unit, and I have been requested to gather information. ? Thank you, Akemi Allison BS, HT(ASCP)HTL E-Mail: akemiat3377@yahoo.com From sgoebel <@t> mirnarx.com Wed Mar 2 13:24:11 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Mar 2 13:24:15 2011 Subject: [Histonet] IHC slides Message-ID: So...I have run down slides and done antigen retrieval on my FFPE slides. They are currently in antigen retrieval that has come to room temperature. I am not going to be able to finish the IHC stains until tomorrow. Will it be better to keep the slides in the antigen retrieval solution at 4 degrees overnight, or put it in buffer at 4 degrees overnight? I really don't want to stay here late. Thanks histo-hotties!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From LSebree <@t> uwhealth.org Wed Mar 2 13:49:13 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Mar 2 13:49:22 2011 Subject: [Histonet] IHC slides In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E27380100292A@UWHC-MAIL01.uwhis.hosp.wisc.edu> We've held slides post-retrieval in buffer (tris, PBS, etc.) overnight with no problem. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Wednesday, March 02, 2011 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC slides So...I have run down slides and done antigen retrieval on my FFPE slides. They are currently in antigen retrieval that has come to room temperature. I am not going to be able to finish the IHC stains until tomorrow. Will it be better to keep the slides in the antigen retrieval solution at 4 degrees overnight, or put it in buffer at 4 degrees overnight? I really don't want to stay here late. Thanks histo-hotties!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fbozkurt <@t> gmail.com Wed Mar 2 14:03:07 2011 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Wed Mar 2 14:03:56 2011 Subject: [Histonet] IHC slides Message-ID: You can put them %3 H2O2 for 10 minutes. and then drop primary antibody and incubate at 4 degrees overnight. From brian <@t> prometheushealthcare.com Wed Mar 2 14:24:32 2011 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Wed Mar 2 14:24:47 2011 Subject: [Histonet] Histolology Supervisor and Tech Openings Sign On and Relocation Message-ID: <015b01cbd917$da3eb910$8ebc2b30$@com> one of the largest, most sophisticated, state-of-the-art laboratories in the Northeast is looking for: both a histology supervisor and a histotech for the 3rd shift with a differential of $3.50 per hour Both positions are eligible for a sign-on bonus (up to 5k) and relocation assistance Positions located in Scarborough, ME Please contact me today for immediate consideration Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From mpence <@t> grhs.net Wed Mar 2 14:51:20 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Mar 2 14:51:24 2011 Subject: [Histonet] IHC slides In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974B5D@is-e2k3.grhs.net> Just hold them in buffer overnight. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Wednesday, March 02, 2011 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC slides So...I have run down slides and done antigen retrieval on my FFPE slides. They are currently in antigen retrieval that has come to room temperature. I am not going to be able to finish the IHC stains until tomorrow. Will it be better to keep the slides in the antigen retrieval solution at 4 degrees overnight, or put it in buffer at 4 degrees overnight? I really don't want to stay here late. Thanks histo-hotties!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Wed Mar 2 15:01:22 2011 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Mar 2 15:01:29 2011 Subject: [Histonet] (no subject) Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C741D@EXCHMBC2.ad.ah.local> Janice Mahoney is retiring from this job and thought you might find it interesting. Coordinator Laboratory Histology Cytology FT Alegent Health System System/Corporate You can view and apply for this job at: http://apply.alegent.com/psc/HRPRD/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_CE.GBL?Page=HRS_CE_JOB_DTL&Action=A&JobOpeningId=38083&SiteId=1&PostingSeq=1 If you experience issues accessing the link above, please go to www.alegent.com and click on Jobs to browse our openings. ________________________________ Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Wed Mar 2 15:29:45 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 2 15:29:48 2011 Subject: [Histonet] IHC slides In-Reply-To: Message-ID: <989930.29614.qm@web65706.mail.ac4.yahoo.com> In buffer at 4?C overnight Ren? J. --- On Wed, 3/2/11, sgoebel@mirnarx.com wrote: From: sgoebel@mirnarx.com Subject: [Histonet] IHC slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 2, 2011, 2:24 PM So...I have run down slides and done antigen retrieval on my FFPE slides.? They are currently in antigen retrieval that has come to room temperature.? I am not going to be able to finish the IHC stains until tomorrow.? Will it be better to keep the slides in the antigen retrieval solution at 4 degrees overnight, or put it in buffer at 4 degrees overnight?? I really don't want to stay here late. Thanks histo-hotties!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mark.Elliott <@t> hli.ubc.ca Wed Mar 2 16:59:17 2011 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Wed Mar 2 16:59:51 2011 Subject: [Histonet] Mouse cardiac myosin antibodies Message-ID: <4D6E5B45020000D60005DF9E@mail.mrl.ubc.ca> I have been asked to stain mouse hearts for cardiac myosin. I have tried the antibody from Abcam (ab50967-Mouse monoclonal to heavy chain cardiac myosin) with no luck. I am using the Mouse on Mouse kit from Biocare and it worked well for me for some other mouse antibodies on mouse tissue but doesn't want to work on this antibody. I have tried different retrieval solutions (pH6 and pH9, as well as Biocare rodent Decloaker). Anyone use this antibody? Any suggestions for another antibody greatly appreciated. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From liz <@t> premierlab.com Wed Mar 2 17:24:34 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Mar 2 17:24:45 2011 Subject: [Histonet] Mouse cardiac myosin antibodies In-Reply-To: <4D6E5B45020000D60005DF9E@mail.mrl.ubc.ca> Message-ID: Mark I took a brief look at the abcam antibody and in particular the review with the paraffin staining. If I was purchasing the antibody I would question the results of that review, since they used a goat anti-mouse detection system which is not ideal for mouse tissue. Plus I'm not sure that the staining pattern is consistent with what I would expect. I would expect to see staining of the myofibrils within the cell. It appears from the image to be staining nuclei and the outside of the cell. I would call Abcam to see if they have tested this antibody internally for paraffin processing or did they completely rely on that review. You could also try enzyme retrieval rather than HIER. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Elliott Sent: Wednesday, March 02, 2011 3:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse cardiac myosin antibodies I have been asked to stain mouse hearts for cardiac myosin. I have tried the antibody from Abcam (ab50967-Mouse monoclonal to heavy chain cardiac myosin) with no luck. I am using the Mouse on Mouse kit from Biocare and it worked well for me for some other mouse antibodies on mouse tissue but doesn't want to work on this antibody. I have tried different retrieval solutions (pH6 and pH9, as well as Biocare rodent Decloaker). Anyone use this antibody? Any suggestions for another antibody greatly appreciated. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From julie.coudenys <@t> ugent.be Thu Mar 3 01:01:41 2011 From: julie.coudenys <@t> ugent.be (julie ) Date: Thu Mar 3 00:56:22 2011 Subject: [Histonet] technovit 9100 Message-ID: <000b01cbd970$d9ac66b0$8d053410$@coudenys@ugent.be> We attempted to cut a knee without decalfication. We saw that we can do that with technovit 9100. This was our protocol: - Fixation with 4% formalin 24h room temp - Ethanol 50% 1h room temp - Ethanol 70% 1 h room temp - Ethanol 80% 1h room temp - Ethanol 96% 1h room temp - Ethanol 96% 1 h room temp - Ethanol 100% 1h room temp - Ethanol 100% 1h room temp - Xyleen 1h room temp - Xyleen 1h room temp - 1/1 xyleen + stabilized technovit 9100 1h room temp - Stabilized technovit 9100 (200ml) + hardener- 1 (1g) 1h room temp - Destabilized* technovit 9100 (200ml) + hardener-1 (1g) 1h 4?C - Destabilized* technovit 9100 (200ml) + hardener-1 (1g) +20g PMMA powder 1h 4?C Polymerisation solution: 90 ml A +10 ml B A: 90ml destabilized technovit 9100 +0.09g hardener-1+14.4g PMMA B: 10ml destabilized technovit 9100+800?l hardener-2+400?l regulator We stir the polymerization solution and we add our samples and we put it in a cooled dessicator for 10min and after that we put it in moulds on -20?C. And wait 24h. We saw that the PMMA powder don?t dissolve. Is that normal? We also attempted that it not polymerizate. Do we have to wait longer? Have we done something wrong? From julie.coudenys <@t> ugent.be Thu Mar 3 01:08:05 2011 From: julie.coudenys <@t> ugent.be (julie ) Date: Thu Mar 3 01:02:46 2011 Subject: [Histonet] technovit 9100 Message-ID: <001001cbd971$be22e1c0$3a68a540$@coudenys@ugent.be> We attempted to cut a knee without decalfication. We saw that we can do that with technovit 9100. This was our protocol: - Fixation with 4% formalin 24h room temp - Ethanol 50% 1h room temp - Ethanol 70% 1 h room temp - Ethanol 80% 1h room temp - Ethanol 96% 1h room temp - Ethanol 96% 1 h room temp - Ethanol 100% 1h room temp - Ethanol 100% 1h room temp - Xyleen 1h room temp - Xyleen 1h room temp - 1/1 xyleen + stabilized technovit 9100 1h room temp - Stabilized technovit 9100 (200ml) + hardener- 1 (1g) 1h room temp - Destabilized* technovit 9100 (200ml) + hardener-1 (1g) 1h 4?C - Destabilized* technovit 9100 (200ml) + hardener-1 (1g) +20g PMMA powder 1h 4?C Polymerisation solution: 90 ml A +10 ml B A: 90ml destabilized technovit 9100 +0.09g hardener-1+14.4g PMMA B: 10ml destabilized technovit 9100+800?l hardener-2+400?l regulator We stir the polymerization solution and we add our samples and we put it in a cooled dessicator for 10min and after that we put it in moulds on -20?C. And wait 24h. We saw that the PMMA powder don?t dissolve. Is that normal? We also attempted that it not polymerizate. Do we have to wait longer? Have we done something wrong? From muralidharmetta <@t> gmail.com Thu Mar 3 03:07:14 2011 From: muralidharmetta <@t> gmail.com (murali) Date: Thu Mar 3 03:07:37 2011 Subject: [Histonet] EGFP expression in mouse testis cryosections Message-ID: dear all, i am relatively new to histochemistry. i would like to visualize EGFP expression mouse testis. i am not sure if this is expressed in cytoplasm or nucleus. what kind of staining i should follow and are there some protocols that can be of help for me. please let me know if i need to provide more details about my experiment. thanks regards murali -- Muralidhar Metta Post Doctoral Fellow Unit of Animal Genomics GIGA-R, B34 +1, Avenue l'hopital 1, 4000 - Li?ge Belgium Phone: off: 0032 - 43664788 GSM: 0032 - 492414482 res: 0032 - 42660723 From histotalk <@t> yahoo.com Thu Mar 3 08:20:13 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Thu Mar 3 08:20:17 2011 Subject: [Histonet] Re: NSH - One Day VIR Forum & VIR Teleconference Series Message-ID: <986680.90101.qm@web120602.mail.ne1.yahoo.com> I have been promoting this NSH program in?my last three shows! Well worth signing up and attending. But, then again all of the NSH conferences are worth attending. :- ) David Kemler, SLS, HT(ASCP)HTL Lab Manager, Gary L. Heller Dermatology Laboratory and Host of www.HistoTALK.com From DKBoyd <@t> chs.net Thu Mar 3 08:34:46 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Thu Mar 3 08:34:53 2011 Subject: [Histonet] bone marrow aspirations In-Reply-To: Message-ID: We let our smears air dry and then we stain them on the Wright stainer, in which the first step is a fixative. We reserve one slide for an FE stain, this slide is fixed in Methanol for 10 mins, then continue per usual with the FE stain. Hope this helps! Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Debra Siena Sent by: histonet-bounces@lists.utsouthwestern.edu 03/02/2011 10:49 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] bone marrow aspirations Hi All, I would appreciate some insight as to how most labs are treating bone marrow smears, in regards to fixation. I am not embarrassed to say that it has been awhile since I worked with them. With the smears are most labs air drying, methanol (alcohol fixation) or spray fixation (pap fixative) or something else? I do thank you for your help. Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsiena@statlab.com | www.statlab.com< http://www.statlab.com/> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From histo20 <@t> hotmail.com Thu Mar 3 09:22:00 2011 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Thu Mar 3 09:22:03 2011 Subject: [Histonet] (no subject) Message-ID: There is a Pathologist Assistant I know who is looking for a position in the Baltimore/Maryland area. If anyone knows of any Pathologist Assistant opportunities in this area, please contact me at wilderpaula@hotmail.com Thank you so much! From AGleiberman <@t> cbiolabs.com Thu Mar 3 11:25:22 2011 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Thu Mar 3 11:25:29 2011 Subject: [Histonet] EGFP expression in mouse testis cryosections In-Reply-To: References: Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C113800@cbiolabs05.CBiolabs.local> Hi Muralidhar, Fix your samples in 4% formaldehyde on PBS (you can prepare it from powder or from 20% solution in water sold by Electron Microscopy Sciences) about 4h at room temp, wash with PBS (at this step you can store your samples at +4 C for several days or even weeks, cryoprotect by incubation in sucrose/PBS solution (10% sucrose until samples sink - usually overnight at +4 C, 30% sucrose until sinking - also usually overnight), transfer into cryomold, fill it with cryo-embedding medium (O.C.T. or other compounds - we are using Neg-50 from Richard-Allan)freeze (you can freeze them fast in mixture or isopentane/dry ice or slow in -80 C freezer - does not matter) and make cryosections. Good GFP expression is well preserved in formaldehyde-fixed material. If fluorescence is low or invisible for some reasons you can stain these sections with either rabbit or chicken anti-GFP antibody from Abcam or chicken anti-GFP antibody from Aves Lab (all of them are good, work in dilutions 1:500 and more) and secondary either AlexaFluor488 or DyLight488 conjugated, up to you. Alternatively, you can embed your samples in paraffin using routine protocol. In this case your native eGFP fluorescence will be lost completely, but you can still visualize eGFP with antibodies mentioned above following heat antigen retrieval - and use either fluorescence or peroxidase technique. I don't remember how high is testis autofluorescence - maybe peroxidase will be better in your case. For brain, intestine and even liver I usually use fluorescence. Good luck. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of murali Sent: Thursday, March 03, 2011 4:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EGFP expression in mouse testis cryosections dear all, i am relatively new to histochemistry. i would like to visualize EGFP expression mouse testis. i am not sure if this is expressed in cytoplasm or nucleus. what kind of staining i should follow and are there some protocols that can be of help for me. please let me know if i need to provide more details about my experiment. thanks regards murali -- Muralidhar Metta Post Doctoral Fellow Unit of Animal Genomics GIGA-R, B34 +1, Avenue l'hopital 1, 4000 - Li?ge Belgium Phone: off: 0032 - 43664788 GSM: 0032 - 492414482 res: 0032 - 42660723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From srishan <@t> mail.holyname.org Thu Mar 3 11:53:40 2011 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Thu Mar 3 11:53:08 2011 Subject: [Histonet] STAINING CONTAINER LIDS Message-ID: We are looking for SAKURA Tissue Tek DRS stainer - container lids. ( This is the dual stainer). Does anyone know who have them. Thanks Nirmala Srishan Histology Supervisor Holy Name Medical Center. Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From KMB01 <@t> grh.org Thu Mar 3 12:47:35 2011 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Thu Mar 3 12:47:40 2011 Subject: [Histonet] Alcian Blue-Acid mucosaccharides with and without hyaluronidase Message-ID: Does anyone out there do this stain? Alcian Blue-Acid mucosaccharides with and without hyaluronidase? Where do you get the testicular hyaluronidase? Thank you so much. This for a student. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. From mcauliff <@t> umdnj.edu Thu Mar 3 12:58:25 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Mar 3 12:55:13 2011 Subject: [Histonet] Alcian Blue-Acid mucosaccharides with and without hyaluronidase In-Reply-To: References: Message-ID: <4D6FE4D1.4090707@umdnj.edu> On 3/3/2011 1:47 PM, Kathy M. Gorham wrote: > Does anyone out there do this stain? Alcian Blue-Acid mucosaccharides > with and without hyaluronidase? Where do you get the testicular > hyaluronidase? Thank you so much. This for a student. > Kathy Gorham H.T. You can get the enzyme from Sigma. Note that there are two different hylaluronidases, bovine testicular and Streptomyces. They have different activities. Geoff > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > Leader in Innovative Excellence 2009 awarded by the OAHHS > Financial Excellence Award 2010 awarded by the national Rural Health Research& Policy Analysis Center > Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet > > GRH Mission > We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. > > > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s only > and should be protected against viewing by unauthorized persons. The information > herein may have been disclosed from records whose confidentiality is protected > by Federal and State Law. Federal regulations prohibit further distribution or > copying of this information without permission. If you received this e-mail > transmission in error, please notify the sender immediately to arrange for return > or destruction of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mward <@t> wfubmc.edu Thu Mar 3 12:54:29 2011 From: mward <@t> wfubmc.edu (Martha Ward) Date: Thu Mar 3 12:57:25 2011 Subject: [Histonet] SOX11 antibody Message-ID: Hello all, I have been asked to try to work up the SOX11 antibody for a research project. The antibody they gave me is from Sigma Life Science. I am having difficulty getting any staining at all. If anyone has any suggestions I would be very grateful. We are using a Leica Bond 3. Thanks in advance for any help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 From brett_connolly <@t> merck.com Thu Mar 3 13:39:15 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Mar 3 13:39:20 2011 Subject: [Histonet] SOX11 antibody In-Reply-To: References: Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D793C830@usctmx1176.merck.com> Hi Martha, I haven't used it, but you can find some info on it from the human protein atlas here: http://www.proteinatlas.org/ENSG00000176887/antibody They recommend 1:100 and you can check out Sigma's recommended procedure for using their Prestige antibodies here: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/ 1/ihc_procedure.Par.0001.File.tmp/ihc_procedure.pdf Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Thursday, March 03, 2011 1:54 PM To: Histonet Subject: [Histonet] SOX11 antibody Hello all, I have been asked to try to work up the SOX11 antibody for a research project. The antibody they gave me is from Sigma Life Science. I am having difficulty getting any staining at all. If anyone has any suggestions I would be very grateful. We are using a Leica Bond 3. Thanks in advance for any help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From tpodawiltz <@t> lrgh.org Thu Mar 3 14:04:09 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Thu Mar 3 14:06:39 2011 Subject: [Histonet] Competancies for handling hazardous material In-Reply-To: References: <000601cbd3c0$d7ebaf80$87c30e80$@grics.net>, , Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DDE30DFB@LRGHEXVS1.practice.lrgh.org> We did a self audit about three or four years ago. My area is considered a small waste storage area and yes there is required training. In New Hampshire, we can get that training through the state. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fortin, Joyce [Joyce.Fortin@uhsinc.com] Sent: Wednesday, March 02, 2011 1:21 PM To: Liz Chlipala; Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material Could I PLEASE have this information, too? I would really appreciate it. Joyce Fortin Histology Supervisor Palmdale Regional Medical Center 38600 Medical Center Drive Palmdale, California 93551 Phone 661-382-5723 Fax 661-382-5747 email: joyce.fortin@uhsinc.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala [liz@premierlab.com] Sent: Wednesday, February 23, 2011 6:51 PM To: Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material Rae We have, you need standard training, but the individuals who inspected our lab did not mention compentances. But our inspection happened because we needed to switch from a conditionally exempt small quantity generator to a small quantitiy generator and we then needed to register with the state, etc. They primarily focused on our waste streams and what chemicals were in the lab. I'm not at work right now, but I'll check tomorrow to see if I have anything that I can share with you. Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rae Staskiewicz Sent: Wed 2/23/2011 6:19 PM To: Histonet Subject: [Histonet] Competancies for handling hazardous material Has anyone been inspected by the EPA regarding removal of hazardous chemicals? It came up in a safety meeting that I should have training and competencies (beyond general safety training and PPE) for pouring xylene from staining dishes and processors into accumulation containers. Rae Ann Staskiewicz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From rmweber113 <@t> comcast.net Thu Mar 3 14:10:25 2011 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Thu Mar 3 14:43:14 2011 Subject: [Histonet] JOB POSITION PENNSYLVANIA In-Reply-To: <1475733675.1595179.1299182774097.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> Message-ID: <723142330.1595527.1299183025242.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net> We have a full time position in a GI practice laboratory in Easton Pennsylvania.? Candidate must have 2 years experience.? Must be proficient in grossing and? have ASCP certification.? Position is Mon-Fri, flexiable hours with benefits. ?Possibility of future supervisor position . ? Interested candidates can fax resume to 570 674-1274 or email to ysarker @comcast.net . Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC From macveigh <@t> usc.edu Thu Mar 3 16:23:50 2011 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Thu Mar 3 16:23:53 2011 Subject: [Histonet] Need to compare prices Message-ID: <000001cbd9f1$ac40ba60$04c22f20$@usc.edu> Hi all, We do Histology and Immunology primarily on liver tissue and only for research. I need to check the prices of other cores and present numbers for comparison as soon as possible. I remember that some time ago there was a question about prices for Histology in a research environment. Most of the responses, however, must have been directed to a personal mail. I would be very thankful if the person who did this research (already) gets in touch with me ( macveigh@usc.edu or 323 442-1188). At the same time, I would appreciate very much all the information that anyone of you can share with me. You can just scan and e-mail your pricelists if this is easier, and please mention the city and state of your location. If you would rather fax it, please write back and I will give you our fax number. Have a nice day and thank you ahead of time Michelle Aloni From mfisher <@t> ecrmc.org Thu Mar 3 16:26:37 2011 From: mfisher <@t> ecrmc.org (Marcia Fisher) Date: Thu Mar 3 16:27:05 2011 Subject: [Histonet] Processing Specimens by CPTs Message-ID: <3ACBB5D73A417547A01970CD3EB55093071D18BF@MAIL1.ecrmc.ci.el-centro.ca.us> Do CPTs (phlebotomist) do any type of specimen processing at your facility? Specifically, if a cytology specimen, for example, one liter of pleural is received in the lab and there are multiple orders for the specimen (cultures, cell count, perhaps a test that has to be sent out, etc), who describes (hazy, opaque, mucoid, etc), splits and aliquots the specimen? CLS, CPT, Histotechs, Cytotechs? We do not have cytologists at our facility at this time but we do a fair amount of non-GYN cytologies. And if anyone knows an answer to this....are CPTs qualified under laboratory regulations, particularly California, to do this type of work? Thanking you in advance. M. Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.   From jnocito <@t> satx.rr.com Thu Mar 3 20:10:52 2011 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Mar 3 20:11:17 2011 Subject: [Histonet] negative controls on immunos In-Reply-To: <4D6E2D35.2B7F.00C9.1@geisinger.edu> References: <4D6E18AB020000C80000F33E@smtp1.gov.pe.ca><92AD9B20A6C38C4587A9FEBE3A30E164081E1BD7BC@CHEXCMS10.one.ads.che.org> <4D6E2D35.2B7F.00C9.1@geisinger.edu> Message-ID: yes. I'm in the beginning stages of validating a new immuno machine. Because we have over 120 antibodies, I'm guessing about 2500 slides. Guess who pays for that testing? Certainly not CAP. I hate monopolies. Even though I know labs who dropped CAP and went with JCAHO, CAP is still a monopoly. Let's revolt. Seems to be catching on in the Middle East. Joe ----- Original Message ----- From: "Angela Bitting" To: "Diana McCaig" ; "Greg Dobbin" ; ; "Joyce Weems" Sent: Wednesday, March 02, 2011 10:42 AM Subject: RE: [Histonet] negative controls on immunos Fun no, impractical yes. >>> "Weems, Joyce" 3/2/2011 10:30 AM >>> The CAP guidelines are pretty clear. Copied from latest checklist.. Isn't this fun???????????!!!!!!!!! j:>) ANP.22570 Phase II N/A YES NO Are appropriate negative controls used? NOTE: Negative controls must assess the presence of nonspecific staining in patient tissue as well as the specificity of each antibody. Results of controls must be documented, either in internal laboratory records, or in the patient report. A statement in the report such as, "All controls show appropriate reactivity" is sufficient. A negative reagent control is used to assess nonspecific or aberrant staining in patient tissue related to the antigen retrieval conditions and/or detection system used. A separate section of patient tissue is processed using the same reagent and epitope retrieval protocol as the patient test slide, except that the primary antibody is omitted, and replaced by any one of the following: ? An unrelated antibody of the same isotype as the primary antibody (for monoclonal primary antibodies) ? An unrelated antibody from the same animal species as the primary antibody (for polyclonal primary antibodies) ? The negative control reagent included in the staining kit ? The diluent/buffer solution in which the primary antibody is diluted In general, a separate negative reagent control should be run for each block of patient tissue being immunostained; however, for cases in which there is simultaneous staining of multiple blocks from the same specimen with the same antibody (e.g., cytokeratin staining of multiple axillary sentinel lymph nodes), performing a single negative control on one of the blocks may be sufficient provided that all such blocks are fixed and processed identically. This exception does not apply to stains on different types of tissues or those using different antigen retrieval protocols or antibody detection systems. The laboratory director must determine which cases will have only one negative reagent control, and this must be specified in the department's procedure manual. The negative reagent control would ideally control for each reagent protocol and antibody retrieval condition; however, large antibody panels often employ multiple antigen retrieval procedures. In such cases, a reasonable minimum control would be to perform the negative reagent control using the most aggressive retrieval procedure in the particular antibody panel. Aggressiveness of antigen retrieval (in decreasing order) is as follows: pressure cooker; enzyme digestion, boiling; microwave; steamer; water bath. High pH retrieval should be considered more aggressive than comparable retrieval in citrate buffer at pH 6.0. It is also important to assess the specificity of each antibody by a negative tissue control, which must show no staining of tissues known to lack the antigen. The negative tissue control is processed using the same fixation, epitope retrieval and immunostaining protocols as the patient tissue. Unexpected positive staining of such tissues indicates that the test has lost specificity, perhaps because of improper antibody concentration or excessive antigen retrieval. Intrinsic properties of the test tissue may also be the cause of "non-specific" staining. For example, tissues with high endogenous biotin activity such as liver or renal tubules may simulate positive staining when using a detection method based on biotin labeling. A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks. These can provide simultaneous positive and negative tissue controls, and are considered "best practice" (see below). 2. The positive control slide or patient test slides, if these slides contain tissue elements that should not react with the antibody. 3. A separate negative tissue control slide. The type of negative tissue control used (i.e., separate sections, internal controls or multitissue blocks) should be specified in the laboratory manual (refer to ANP.22250). Multitissue blocks may be considered best practice and can have a major role in maintaining quality. When used as a combined positive and negative tissue control as mentioned above, they can serve as a permanent record documenting the sensitivity and specificity of every stain, particularly when mounted on the same slide as the patient tissue. When the components are chosen appropriately, multitissue blocks may be used for many different primary antibodies, decreasing the number of different control blocks needed by the laboratory. Multitissue blocks are also ideal for determining optimal titers of primary antibodies since they allow simultaneous evaluation of many different pieces of tissue. Finally, they are a useful and efficient means to screen new antibodies for sensitivity and specificity or new lots of antibody for consistency, which should be done before putting any antibody into diagnostic use. REFERENCES 1) Leong AS-Y, Cooper K, Leong FJW-M. Manual of Diagnostic Antibodies for Immunohistology. 2nd ed. London: Greenwich Medical Media; 2003 2) Dabbs DJ. Diagnostic Immunohistochemistry. New York: Churchill Livingstone; 2002 3) Burry RW. Specificity controls for immunocytochemical methods. J Histochem Cytochem 2000;48:163-166 Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Wednesday, March 02, 2011 09:15 To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] negative controls on immunos Yes, it is always best to run every conceivable control available. Then you can be really, really, really sure. However, if you have practical issues that come into play such as cost of reagents, or extra controls taking up valuable space on the stainer then you might have to think about what you are trying to accomplish with each control and balance the cost vs benefit. So what are we checking with the negative control? We are checking the reagents to ensure that there is no non-specific reaction arising from the detection kit or the buffers, etc. If you are running the same block tomorrow with the same detection kit (ie same lot) it is not necessary (in my humble opinion) to check it again with another negative control. I suppose if you are worried that someone could be trying to sabotage your work by sneaking into your lab at night and contaminating your detection kit...then the simplest way to detect this sinister activity would be to run a negative control every time. Otherwise, I think one negative control slide per block per detection kit will be adequate. Disclaimer: Please know that I have just tried to inject a little humor into this response. I am really not trying to be sarcastic in anyway. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Diana McCaig" 3/1/2011 2:07 PM >>> If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Mar 4 06:12:48 2011 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Mar 4 06:12:52 2011 Subject: [Histonet] Seeking contingent-PT histotech work- Columbus, OH Message-ID: HTL (ASCP) certified histotechnologist seeking contingent or part-time bench position. I am completing Master's and will be seeking a contingent or part-time bench histology position to round out my schedule. I have extensive routine histology experience, manual and automated special stains, frozen section, manual and automated IHC, and manual and automated IF experience. I have worked in both large-high volume, and smaller histology labs, reference environments, and some research experience. I will be available some evenings, some mornings, every other weekend and holidays beginning at the end of April. I am fulfilling a faculty contract that will end in late June, so I may have more availability after that. If you have a need for a fill-in person, please contact me at joelleweaver@hotmail.com. Thank you Joelle Weaver Joelle Weaver HTL (ASCP) Histology Instructor, Program Director Columbus State Community College 614-287-2217 From kaylee <@t> medwrench.com Fri Mar 4 07:48:44 2011 From: kaylee <@t> medwrench.com (Kaylee McCaffrey) Date: Fri Mar 4 07:48:51 2011 Subject: [Histonet] TBS ATP1 Manual Message-ID: Does anyone have a technical manual for the ATP1? Kaylee McCaffrey MedWrench Product Manager kaylee@medwrench.com From jbirkner <@t> colabserv.com Fri Mar 4 08:46:54 2011 From: jbirkner <@t> colabserv.com (Jeff Birkner) Date: Fri Mar 4 08:47:01 2011 Subject: [Histonet] Competancies for handling hazardous material In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DDE30DFB@LRGHEXVS1.practice.lrgh.org> Message-ID: I have reviewed 40 CFR Part 262 and the only training requirements that I see are the RCRA for Large Quantity generators and those for university or academic institutions. I do not see any requirements for medical laboratories that fall outside of the above generator/institution types. Can someone point me in the right direction if this is not correct? Thanks! Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirkner@colabserv.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, March 03, 2011 2:04 PM To: Fortin, Joyce; Liz Chlipala; Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material We did a self audit about three or four years ago. My area is considered a small waste storage area and yes there is required training. In New Hampshire, we can get that training through the state. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fortin, Joyce [Joyce.Fortin@uhsinc.com] Sent: Wednesday, March 02, 2011 1:21 PM To: Liz Chlipala; Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material Could I PLEASE have this information, too? I would really appreciate it. Joyce Fortin Histology Supervisor Palmdale Regional Medical Center 38600 Medical Center Drive Palmdale, California 93551 Phone 661-382-5723 Fax 661-382-5747 email: joyce.fortin@uhsinc.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala [liz@premierlab.com] Sent: Wednesday, February 23, 2011 6:51 PM To: Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material Rae We have, you need standard training, but the individuals who inspected our lab did not mention compentances. But our inspection happened because we needed to switch from a conditionally exempt small quantity generator to a small quantitiy generator and we then needed to register with the state, etc. They primarily focused on our waste streams and what chemicals were in the lab. I'm not at work right now, but I'll check tomorrow to see if I have anything that I can share with you. Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rae Staskiewicz Sent: Wed 2/23/2011 6:19 PM To: Histonet Subject: [Histonet] Competancies for handling hazardous material Has anyone been inspected by the EPA regarding removal of hazardous chemicals? It came up in a safety meeting that I should have training and competencies (beyond general safety training and PPE) for pouring xylene from staining dishes and processors into accumulation containers. Rae Ann Staskiewicz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. From mward <@t> wfubmc.edu Fri Mar 4 10:43:25 2011 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Mar 4 10:43:44 2011 Subject: [Histonet] Direct costs for small biopsy processing Message-ID: I am posted this question for a colleague. She has been asked to provide the direct costs for processing one biopsy, for example, a punch skin biopsy. Would anyone be willing to share that information with us? She is looking at the total costs of having a resident or PA gross, someone to accession the case, the tissue processing, embedding, microtomy, staining the H&E, coverslipping and reviewing the slide. We know that reagents and salary costs will vary but she just wants a ball park figure for comparison. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 From liz <@t> premierlab.com Fri Mar 4 10:51:59 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Mar 4 10:52:03 2011 Subject: [Histonet] Competancies for handling hazardous material In-Reply-To: Message-ID: Jeff Those are the federal Reqs. You have state reqs that you need to meet also. When we were inspected by the Colorado Department of Public Health they told us that we had to have training and that it did not matter how many employees that I had since I was now considered a small quantity generator verses a conditionally exempt small quantity generator. EPA is going to be different than OSHA. With OSHA you have to have 10 or more employees to be subject to the reqs. I have less than 10 employees, but we perform training on everything and have a considerable safety manual that includes practically everything. One other thing, if you are a small business the government will provide you with assistance you just need to go to the OSHA website, they may also provide assistance to others. We had a voluntary inspection from OSHA. It took a while to happen since we are not considered a high risk workplace, but it was helpful. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Jeff Birkner [mailto:jbirkner@colabserv.com] Sent: Friday, March 04, 2011 7:47 AM To: Podawiltz, Thomas; Fortin, Joyce; Liz Chlipala; Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material I have reviewed 40 CFR Part 262 and the only training requirements that I see are the RCRA for Large Quantity generators and those for university or academic institutions. I do not see any requirements for medical laboratories that fall outside of the above generator/institution types. Can someone point me in the right direction if this is not correct? Thanks! Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirkner@colabserv.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, March 03, 2011 2:04 PM To: Fortin, Joyce; Liz Chlipala; Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material We did a self audit about three or four years ago. My area is considered a small waste storage area and yes there is required training. In New Hampshire, we can get that training through the state. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fortin, Joyce [Joyce.Fortin@uhsinc.com] Sent: Wednesday, March 02, 2011 1:21 PM To: Liz Chlipala; Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material Could I PLEASE have this information, too? I would really appreciate it. Joyce Fortin Histology Supervisor Palmdale Regional Medical Center 38600 Medical Center Drive Palmdale, California 93551 Phone 661-382-5723 Fax 661-382-5747 email: joyce.fortin@uhsinc.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala [liz@premierlab.com] Sent: Wednesday, February 23, 2011 6:51 PM To: Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material Rae We have, you need standard training, but the individuals who inspected our lab did not mention compentances. But our inspection happened because we needed to switch from a conditionally exempt small quantity generator to a small quantitiy generator and we then needed to register with the state, etc. They primarily focused on our waste streams and what chemicals were in the lab. I'm not at work right now, but I'll check tomorrow to see if I have anything that I can share with you. Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rae Staskiewicz Sent: Wed 2/23/2011 6:19 PM To: Histonet Subject: [Histonet] Competancies for handling hazardous material Has anyone been inspected by the EPA regarding removal of hazardous chemicals? It came up in a safety meeting that I should have training and competencies (beyond general safety training and PPE) for pouring xylene from staining dishes and processors into accumulation containers. Rae Ann Staskiewicz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. From liz <@t> premierlab.com Fri Mar 4 11:09:42 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Mar 4 11:09:47 2011 Subject: [Histonet] Competancies for handling hazardous material In-Reply-To: Message-ID: One quick correction you may have state reqs, not all states do. Here is what I took from Janet and Dick Dapson's book 3rd Edition which is from 1995. In 1995 Iowa did not have a state office but the Regional EPA office number is (913) 551-7050 or (800) 223-0423. I also thought it was odd that it was the Colorado Department of Public Health that inspected me, but when I looked in the book that's who they listed at the States Hazardous Waste Management Agency. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Friday, March 04, 2011 9:52 AM To: Jeff Birkner; Podawiltz, Thomas; Fortin, Joyce; Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material Jeff Those are the federal Reqs. You have state reqs that you need to meet also. When we were inspected by the Colorado Department of Public Health they told us that we had to have training and that it did not matter how many employees that I had since I was now considered a small quantity generator verses a conditionally exempt small quantity generator. EPA is going to be different than OSHA. With OSHA you have to have 10 or more employees to be subject to the reqs. I have less than 10 employees, but we perform training on everything and have a considerable safety manual that includes practically everything. One other thing, if you are a small business the government will provide you with assistance you just need to go to the OSHA website, they may also provide assistance to others. We had a voluntary inspection from OSHA. It took a while to happen since we are not considered a high risk workplace, but it was helpful. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Jeff Birkner [mailto:jbirkner@colabserv.com] Sent: Friday, March 04, 2011 7:47 AM To: Podawiltz, Thomas; Fortin, Joyce; Liz Chlipala; Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material I have reviewed 40 CFR Part 262 and the only training requirements that I see are the RCRA for Large Quantity generators and those for university or academic institutions. I do not see any requirements for medical laboratories that fall outside of the above generator/institution types. Can someone point me in the right direction if this is not correct? Thanks! Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirkner@colabserv.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, March 03, 2011 2:04 PM To: Fortin, Joyce; Liz Chlipala; Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material We did a self audit about three or four years ago. My area is considered a small waste storage area and yes there is required training. In New Hampshire, we can get that training through the state. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fortin, Joyce [Joyce.Fortin@uhsinc.com] Sent: Wednesday, March 02, 2011 1:21 PM To: Liz Chlipala; Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material Could I PLEASE have this information, too? I would really appreciate it. Joyce Fortin Histology Supervisor Palmdale Regional Medical Center 38600 Medical Center Drive Palmdale, California 93551 Phone 661-382-5723 Fax 661-382-5747 email: joyce.fortin@uhsinc.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala [liz@premierlab.com] Sent: Wednesday, February 23, 2011 6:51 PM To: Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material Rae We have, you need standard training, but the individuals who inspected our lab did not mention compentances. But our inspection happened because we needed to switch from a conditionally exempt small quantity generator to a small quantitiy generator and we then needed to register with the state, etc. They primarily focused on our waste streams and what chemicals were in the lab. I'm not at work right now, but I'll check tomorrow to see if I have anything that I can share with you. Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rae Staskiewicz Sent: Wed 2/23/2011 6:19 PM To: Histonet Subject: [Histonet] Competancies for handling hazardous material Has anyone been inspected by the EPA regarding removal of hazardous chemicals? It came up in a safety meeting that I should have training and competencies (beyond general safety training and PPE) for pouring xylene from staining dishes and processors into accumulation containers. Rae Ann Staskiewicz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Fri Mar 4 11:11:36 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Mar 4 11:11:43 2011 Subject: [Histonet] GSH Annual Meeting at Callaway Gardens, Georgia Message-ID: <9BF995BC0E47744E9673A41486E24EE22DBB6912FC@MERCERMAIL.MercerU.local> Hi Histo Friends, Our meeting is only 3 weeks away, make your plans if you have not already done so. You can still register for the meeting right up until it begins, but make your hotel reservations now before the price goes up. Location is The Mountain Creek Inn, Callaway Gardens, Pine Mountain, Georgia, call for hotel reservations at 1-800-225-5292 before all the discounted rooms are gone. Also go to www.histosearch.com/gsh and on the symposium page download your program. Vendors can find their registration form on the same page. Room rates start at $99 (IF ROOMS ARE STILL AVAILABLE) plus tax which includes Continental Breakfast and Admission to the Park. For more information about things to do at Callaway click on this link: http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx Our theme this year is "METAMORPHOSIS: Transforming Histotechs." If anyone has questions, please contact me for assistance. Come TRANSFORM yourselves. Shirley Powell GSH Secretary Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From kmerriam2003 <@t> yahoo.com Fri Mar 4 12:24:57 2011 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Mar 4 12:25:01 2011 Subject: [Histonet] Senior Histologist/Lab Manager position at AstraZeneca in Waltham, MA Message-ID: <25846.9407.qm@web130124.mail.mud.yahoo.com> Hello all, ? Below is a great job opportunity for a senior histologist/lab manager at AstraZeneca in Waltham, MA.? I am posting this for a colleague, so please do not email me if you are interested. ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ? http://jobs.astrazeneca.com/jobs/39-histotechnologistscientist ? Here is the job description: ? Histotechnologist/Scientist Reference Number ? 601367 Date Added ? 18/02/2011 Category ? Research and Non-Clinical Develo... Company ? AstraZeneca Country ? United States Location ? Massachusetts (Waltham) ? Incumbent lead a small Histology Laboratory operating in a discovery and safety assessment environment and assist in the conduct of experiments/studies that will include investigative, problem-solving, and pre-development studies in support of research teams. Major Responsibilities: * As a working supervisor, provide day-to-day management of the Histology Laboratory and Necropsy Suite * Provide leadership, supervision, and mentoring to histology team members * Work closely with the histologists, pathologists, research scientists, and toxicologists within Safety Assessment US and Global Safety Assessment regarding study plan development, completion and reporting * Ensure smooth operations and timely completion of necropsies, trimming, embedding and H&E staining, quality control, and other histology procedures such as frozen sections, immunohistochemistry (IHC) and in situ hybridization (ISH) * Develop new histology, necropsy, and IHC procedures as necessary * Perform large and small animal necropsies * Ensure Histology Laboratory and Necropsy Suites have appropriate standard operating and safety procedures in place and that staff are compliant * Serve on some studies as the histologist responsible for study specific support (Histology Study Coordinator) * Be familiar with and provide local management of histology and pathology-related data systems (e.g., PathData?) * Maintain adequate and appropriate supplies for the histology laboratory and necropsy suite Requirements Qualifications: Skills, Knowledge/Education/Experience * Bachelor of Science (BS), MS desirable * Certified Histology Technician (HT/HTL) * Minimum 7 years industry experience * Laboratory management experience * Knowledge and familiarity with histology laboratory equipment and all phases of operations of a histology laboratory * Experience with large and small animal necropsy * Experience with special stains, plastic-embedding and molecular techniques such as immunohistochemistry * Experience in working with and managing Standard Operating Procedures (SOPs) * Knowledge of Good Laboratory Practices (GLP) standards, laboratory safety procedures, and chemical hygiene management * Enthusiastic, self-motivated, leadership and mentoring skills * Excellent communication skills (written and oral) From shu-cheng.chen <@t> merck.com Fri Mar 4 13:53:02 2011 From: shu-cheng.chen <@t> merck.com (Chen, Shu-Cheng) Date: Fri Mar 4 13:53:07 2011 Subject: [Histonet] hamster tissue processing Message-ID: <5D62649615FAA6478F801A08D10E51855A8B30CCA8@USCTMXP51003.merck.com> Hi, We need to process formalin fixed hamster guts, which we have never done before. Anybody has a protocol to share with us is very much appreciated. Can we use protocol for rat? Also, is there any marker for hamster lymphocytes or macrophages? Thanks, Shu-Cheng Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Timothy.Morken <@t> ucsfmedctr.org Fri Mar 4 14:37:36 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Mar 4 14:37:53 2011 Subject: [Histonet] Real science for real people (and their food) Message-ID: Sometimes science helps us in the real world: February 28, 2011 The 5-Second Rule By C. CLAIBORNE RAY Q. You know the five-second rule for dropped food? Is it really safe if you pick it up in time? A. "The five-second rule probably should become the zero-second rule," said Dr. Roy M. Gulick, chief of the division of infectious diseases at Weill Cornell Medical College. "Eating dropped food poses a risk for ingestion of bacteria and subsequent gastrointestinal disease, and the time the food sits on the floor does not change the risk." In general, if there are bacteria on the floor, they will cling to the food nearly immediately on contact, Dr. Gulick said. Factors that influence the risk and the rate of bacterial transfer include the type of floor; the type of food; the type of bacteria; and how long the bacteria have been on the floor. In a study published in 2007 in The Journal of Applied Microbiology, Clemson University researchers tested salmonella placed on wood, tile or carpet, and dropped bologna on the surfaces for 5, 30 or 60 seconds. With both wood and tile, more than 99 percent of the bacteria were transferred nearly immediately, and there was no difference by the time of contact. Carpet transferred a smaller number of bacteria, again with no difference by contact time. The amount transferred decreased over hours, but there were still thousands of the bacteria per square centimeter on the surfaces after 24 hours, and hundreds survived on the surfaces for as long as four weeks. As few as 10 salmonella bacteria can cause gastroenteritis.C. CLAIBORNE RAY Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org From Timothy.Morken <@t> ucsfmedctr.org Fri Mar 4 14:44:43 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Mar 4 14:44:53 2011 Subject: [Histonet] RE: Real science for real people (and their food) revised format In-Reply-To: References: Message-ID: This is easier to read ***************************************** The 5-Second Rule By C. CLAIBORNE RAY Feb 28, 2011, NY Times Q. You know the five-second rule for dropped food? Is it really safe if you pick it up in time? A. ?The five-second rule probably should become the zero-second rule,? said Dr. Roy M. Gulick, chief of the division of infectious diseases at Weill Cornell Medical College. ?Eating dropped food poses a risk for ingestion of bacteria and subsequent gastrointestinal disease, and the time the food sits on the floor does not change the risk.? In general, if there are bacteria on the floor, they will cling to the food nearly immediately on contact, Dr. Gulick said. Factors that influence the risk and the rate of bacterial transfer include the type of floor; the type of food; the type of bacteria; and how long the bacteria have been on the floor. In a study published in 2007 in The Journal of Applied Microbiology, Clemson University researchers tested salmonella placed on wood, tile or carpet, and dropped bologna on the surfaces for 5, 30 or 60 seconds. With both wood and tile, more than 99 percent of the bacteria were transferred nearly immediately, and there was no difference by the time of contact. Carpet transferred a smaller number of bacteria, again with no difference by contact time. The amount transferred decreased over hours, but there were still thousands of the bacteria per square centimeter on the surfaces after 24 hours, and hundreds survived on the surfaces for as long as four weeks. As few as 10 salmonella bacteria can cause gastroenteritis. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, March 04, 2011 12:38 PM To: Histonet Subject: [Histonet] Real science for real people (and their food) Sometimes science helps us in the real world: February 28, 2011 The 5-Second Rule By C. CLAIBORNE RAY Q. You know the five-second rule for dropped food? Is it really safe if you pick it up in time? A. "The five-second rule probably should become the zero-second rule," said Dr. Roy M. Gulick, chief of the division of infectious diseases at Weill Cornell Medical College. "Eating dropped food poses a risk for ingestion of bacteria and subsequent gastrointestinal disease, and the time the food sits on the floor does not change the risk." In general, if there are bacteria on the floor, they will cling to the food nearly immediately on contact, Dr. Gulick said. Factors that influence the risk and the rate of bacterial transfer include the type of floor; the type of food; the type of bacteria; and how long the bacteria have been on the floor. In a study published in 2007 in The Journal of Applied Microbiology, Clemson University researchers tested salmonella placed on wood, tile or carpet, and dropped bologna on the surfaces for 5, 30 or 60 seconds. With both wood and tile, more than 99 percent of the bacteria were transferred nearly immediately, and there was no difference by the time of contact. Carpet transferred a smaller number of bacteria, again with no difference by contact time. The amount transferred decreased over hours, but there were still thousands of the bacteria per square centimeter on the surfaces after 24 hours, and hundreds survived on the surfaces for as long as four weeks. As few as 10 salmonella bacteria can cause gastroenteritis.C. CLAIBORNE RAY Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbarone <@t> NEMOURS.ORG Fri Mar 4 14:56:17 2011 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Fri Mar 4 14:56:21 2011 Subject: [Histonet] Beecher Instruments Message-ID: Histonetters...It has been a longtime concern of mine that the only company offering TMA instrumentation was Beecher Instruments.....and the wait list for those, was totally unacceptable. It came to my attention today, from a reliable source at a university.... that Beecher has gone out of business...( after buying Chemicon's arrayer...and taking away the only real alternative). I have heard from the same reliable source they may be purchased by a foreign investor...my prayer is that perhaps that could be Leica! Does anyone have more info on this? I wonder what this means for those who already have one.....at least I am on the wait list! We have tried several manual systems from molds to punches....and there seems no real competition from these, which are difficult to control or expensive to use. What is the word out there....suggestions for us "wait-ors"..... CB From bouchalr <@t> wvuhealthcare.com Fri Mar 4 15:00:29 2011 From: bouchalr <@t> wvuhealthcare.com (Bouchal, Rena L) Date: Fri Mar 4 14:59:50 2011 Subject: [Histonet] job description help? Message-ID: I am re-writing several job descriptions and can use some input. I need titles of similar positions at other institutions so we can research salaries. What are your titles (and if you can share) salary ranges for these please? 1) We have Gross Room techs who cut and stain frozens, accession all specimens, prepare cassettes, call about all improper submissions, prepare grossing stations, cleanup after everyone, handle maintenance of cryostat and cassette labelers, ordering, scheduling, etc. Our LAB TECH title just doesn't seem good enough to cover tasks and stress level of the gross room. 2) We have morgue/forensic/autopsy positions (3). Two of them assist in autopsies, prepare all autopsy rooms, assist in forensic collection, dump hospital specimens after appropriate time, order, assist in training of PA's and residents, body removal from the floor, release of bodies to funeral homes and ME cases, etc. 3) I am hiring a 3rd to oversee the autopsy area(since we will be doubling volume), do all the above tasks in #2 plus scheduling, interact w/ removal services, attend CORE, gift registry meetings, etc. We will have a large ME volume (apx 500/yr) so this will be coordinated also. Any input would be greatly appreciated. p.s. We are also looking for a Technical Specialist in Histology.. BS or BA... HTL... QIHC or QIHC eligible. Our salary is very competitive. Rena Bouchal, M.S. Anatomic Pathology Manager West Virginia University Hospitals 304-293-7765 ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From vkline2344 <@t> comcast.net Fri Mar 4 15:04:38 2011 From: vkline2344 <@t> comcast.net (vkline2344) Date: Fri Mar 4 15:05:07 2011 Subject: [Histonet] Employment Search Recommendations Message-ID: Dear Fellow Histology Professionals, I am a Histotechnician student currently finishing up the final few months in my Histology Technician program and will be graduating in early June. I am planning to begin my employment search now and wanted to ask if anyone has any specific recommendations for websites they'd recommend for employment searches? I've been keeping my eye on the employment listings in Advance Magazine and on the ASCP and NSH websites, as well as Indeed and CareerBuilder. If there are other websites you would highly recommend or even histology headhunters or recruiters, I'd sure appreciate any advice or suggestions. I currently live in Lancaster, Pennsylvania and my job search will primarily be focused on, but not limited to: SE Pennsylvania (Lancaster, Philadelphia, Harrisburg, Reading, York, Gettysburg), Delaware and Maryland. Thanks in advance to any who reply! Victoria Kline Histology Technician Student Lancaster, PA vkline2344@comcast.net From hfedor <@t> jhmi.edu Fri Mar 4 15:09:34 2011 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Fri Mar 4 15:09:39 2011 Subject: [Histonet] RE: Beecher Instruments In-Reply-To: References: Message-ID: Hello, There is a company that I just ran a few months ago. http://www.pathologydevices.com/TMArrayer.htm they have a manual arrayer that is running about 30,000.00. A little more than the Beecher Arrayer but it does offer some improvements. They are also able to sell the Needles that will fit into the Beecher instrument. I have spoken to Ron Gebing at Pathology Devices and he is responsive and reachable. I am very optimistic, He has many units in use in the U.S. and the world. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St,?| Marburg Room 406 Baltimore, MD?| 21287-7065 410.614.1660 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol Sent: Friday, March 04, 2011 3:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Beecher Instruments Importance: High Histonetters...It has been a longtime concern of mine that the only company offering TMA instrumentation was Beecher Instruments.....and the wait list for those, was totally unacceptable. It came to my attention today, from a reliable source at a university.... that Beecher has gone out of business...( after buying Chemicon's arrayer...and taking away the only real alternative). I have heard from the same reliable source they may be purchased by a foreign investor...my prayer is that perhaps that could be Leica! Does anyone have more info on this? I wonder what this means for those who already have one.....at least I am on the wait list! We have tried several manual systems from molds to punches....and there seems no real competition from these, which are difficult to control or expensive to use. What is the word out there....suggestions for us "wait-ors"..... CB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Mar 4 15:19:51 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Mar 4 15:19:55 2011 Subject: [Histonet] RE: Beecher Instruments In-Reply-To: References: Message-ID: There is also the Sakuro Quick-Ray System. I don't know if you've tried that already, but I've used it in the past and liked it. http://www.sakura-americas.com/products/tisstek-quickray.html The list price is $3950 for the whole kit and the recipient blocks are $68 each regardless of size (1 mm, 2 mm, 3 mm, or 5 mm). Mark On Fri, Mar 4, 2011 at 1:09 PM, Helen Fedor wrote: > Hello, There is a company that I just ran a few months ago. > http://www.pathologydevices.com/TMArrayer.htm they have a manual arrayer > that is running about 30,000.00. A little more than the Beecher Arrayer but > it does offer some improvements. They are also able to sell the Needles that > will fit into the Beecher instrument. I have spoken to Ron Gebing at > Pathology Devices and he is responsive and reachable. > > I am very optimistic, He has many units in use in the U.S. and the world. > > > > Helen L. Fedor > > Tissue Microarray Lab, Manager > Prostate Spore Lab, Manager > Johns Hopkins University > 600 N. Wolfe St, | Marburg Room 406 > Baltimore, MD | 21287-7065 > > 410.614.1660 > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol > Sent: Friday, March 04, 2011 3:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Beecher Instruments > Importance: High > > Histonetters...It has been a longtime concern of mine that the only > company offering TMA instrumentation was Beecher Instruments.....and the > wait list for those, was totally unacceptable. It came to my attention > today, from a reliable source at a university.... that Beecher has gone > out of business...( after buying Chemicon's arrayer...and taking away > the only real alternative). I have heard from the same reliable source > they may be purchased by a foreign investor...my prayer is that perhaps > that could be Leica! Does anyone have more info on this? I wonder what > this means for those who already have one.....at least I am on the wait > list! We have tried several manual systems from molds to punches....and > there seems no real competition from these, which are difficult to > control or expensive to use. What is the word out there....suggestions > for us "wait-ors"..... CB > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From hfedor <@t> jhmi.edu Fri Mar 4 15:31:02 2011 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Fri Mar 4 15:31:10 2011 Subject: [Histonet] RE: Beecher Instruments In-Reply-To: References: Message-ID: We too have tried the Quick Ray, and I think it is fine if you are not in the business. But if you only make a few arrays at a time then it is really wonderful I think for making small control block TMA's it is perfect. You can cut down the size of the recipient blocks to be able to make several out of each of these. The resultant blocks cut like butter. You don't lose a single section and you can cut them easily at 3 microns. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 410.614.1660 From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Friday, March 04, 2011 4:20 PM To: Helen Fedor Cc: Barone, Carol; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Beecher Instruments There is also the Sakuro Quick-Ray System. I don't know if you've tried that already, but I've used it in the past and liked it. http://www.sakura-americas.com/products/tisstek-quickray.html The list price is $3950 for the whole kit and the recipient blocks are $68 each regardless of size (1 mm, 2 mm, 3 mm, or 5 mm). Mark On Fri, Mar 4, 2011 at 1:09 PM, Helen Fedor > wrote: Hello, There is a company that I just ran a few months ago. http://www.pathologydevices.com/TMArrayer.htm they have a manual arrayer that is running about 30,000.00. A little more than the Beecher Arrayer but it does offer some improvements. They are also able to sell the Needles that will fit into the Beecher instrument. I have spoken to Ron Gebing at Pathology Devices and he is responsive and reachable. I am very optimistic, He has many units in use in the U.S. and the world. Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 410.614.1660 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol Sent: Friday, March 04, 2011 3:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Beecher Instruments Importance: High Histonetters...It has been a longtime concern of mine that the only company offering TMA instrumentation was Beecher Instruments.....and the wait list for those, was totally unacceptable. It came to my attention today, from a reliable source at a university.... that Beecher has gone out of business...( after buying Chemicon's arrayer...and taking away the only real alternative). I have heard from the same reliable source they may be purchased by a foreign investor...my prayer is that perhaps that could be Leica! Does anyone have more info on this? I wonder what this means for those who already have one.....at least I am on the wait list! We have tried several manual systems from molds to punches....and there seems no real competition from these, which are difficult to control or expensive to use. What is the word out there....suggestions for us "wait-ors"..... CB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Mar 4 16:00:41 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Mar 4 16:01:01 2011 Subject: [Histonet] RE: Beecher Instruments In-Reply-To: References: Message-ID: Carol, K7 biosystems / Estigen instruments is what you heard about. We bought ours from K7 last summer but apparently Estigen has taken over their operations of TMA equipment. http://k7bio.com/ http://www.estigen.com/ Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol Sent: Friday, March 04, 2011 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Beecher Instruments Importance: High Histonetters...It has been a longtime concern of mine that the only company offering TMA instrumentation was Beecher Instruments.....and the wait list for those, was totally unacceptable. It came to my attention today, from a reliable source at a university.... that Beecher has gone out of business...( after buying Chemicon's arrayer...and taking away the only real alternative). I have heard from the same reliable source they may be purchased by a foreign investor...my prayer is that perhaps that could be Leica! Does anyone have more info on this? I wonder what this means for those who already have one.....at least I am on the wait list! We have tried several manual systems from molds to punches....and there seems no real competition from these, which are difficult to control or expensive to use. What is the word out there....suggestions for us "wait-ors"..... CB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mark.Burton <@t> leica-microsystems.com Fri Mar 4 16:01:14 2011 From: Mark.Burton <@t> leica-microsystems.com (Mark.Burton@leica-microsystems.com) Date: Fri Mar 4 16:01:24 2011 Subject: [Histonet] Mark Burton is out of the office. Message-ID: I will be out of the office starting 03/04/2011 and will not return until 03/10/2011. I will have limited access to email. For immediate assistance please contact Sarah Holt (603)496-7253 sarah.holt@leica-microsystems.com ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From dwenzel01 <@t> gmail.com Fri Mar 4 16:40:28 2011 From: dwenzel01 <@t> gmail.com (Dawn Herron) Date: Fri Mar 4 16:40:32 2011 Subject: [Histonet] TSH symposium Message-ID: Attention fellow Texas Histotechs! I am interested in going to the TSH symposium at the beginning of April but I can't get a hold of the paperwork to register. I'd like to get it ASAP so I can get the travel paperwork started at my workplace and get a reservation at the hotel and not pay the late registration fee. The website has a link but it is down. I tried the generic TSH email but no one has responded. There is not a phone number on the website to call. Does anyone out there know how I can contact TSH and get registered and get a copy of the program and registration form for the 2011 symposium? Thanks so much! Dawn Herron From clb1158 <@t> yahoo.com Fri Mar 4 17:06:21 2011 From: clb1158 <@t> yahoo.com (C B) Date: Fri Mar 4 17:06:24 2011 Subject: [Histonet] immuno stain for nerve in arterial wall Message-ID: <594300.54651.qm@web114017.mail.gq1.yahoo.com> We had an investigator inquire about flourescent immuno stains for?nerve in arterial wall (not sure what species they are working with).? I've never worked with nerve before.? Can someone give me some advice about what primary should I be searching for?? I realize I have to look in to species cross reactivity once I get that information, but I don't even know what to "search" for. Thank you, Cindy Baranowski SJTRI Atlanta, GA From hlukey <@t> msn.com Fri Mar 4 17:59:57 2011 From: hlukey <@t> msn.com (Hugh Luk) Date: Fri Mar 4 18:00:01 2011 Subject: [Histonet] RE: Beecher Instruments In-Reply-To: References: , Message-ID: Hi Carol, We have both the Pathology Devices TMArrayer and the Beecher MTA-1. The needles are not interchangeable, but they are similar (the notch is inverted and will not fit the into the slot). Our collaborators at NCI's Tissue Array Research Program recommended Pathology Devices to us. Their "semi-automated" TMArrayer is easier to use than the MTA-1, and I echo Helen's thoughts that Ron Geging is true-to-his-word, helpful and responds quickly. We purchased our unit almost 3 years ago, and have had only positive uses from it. For non-research histology labs, Pathology Devices is pricey. However, if you can afford it and make use of it, I recommend it. Sad to hear about Beecher. Have a nice weekend. Hugh Luk Pathology Shared Resources Lab Manager University of Hawaii Cancer Center 1236 Lauhala Street Honolulu, HI 96813 > From: hfedor@jhmi.edu > To: cbarone@NEMOURS.ORG; histonet@lists.utsouthwestern.edu > Date: Fri, 4 Mar 2011 16:09:34 -0500 > CC: > Subject: [Histonet] RE: Beecher Instruments > > Hello, There is a company that I just ran a few months ago. http://www.pathologydevices.com/TMArrayer.htm they have a manual arrayer that is running about 30,000.00. A little more than the Beecher Arrayer but it does offer some improvements. They are also able to sell the Needles that will fit into the Beecher instrument. I have spoken to Ron Gebing at Pathology Devices and he is responsive and reachable. > > I am very optimistic, He has many units in use in the U.S. and the world. > > > > Helen L. Fedor > > Tissue Microarray Lab, Manager > Prostate Spore Lab, Manager > Johns Hopkins University > 600 N. Wolfe St, | Marburg Room 406 > Baltimore, MD | 21287-7065 > > 410.614.1660 > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barone, Carol > Sent: Friday, March 04, 2011 3:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Beecher Instruments > Importance: High > > Histonetters...It has been a longtime concern of mine that the only > company offering TMA instrumentation was Beecher Instruments.....and the > wait list for those, was totally unacceptable. It came to my attention > today, from a reliable source at a university.... that Beecher has gone > out of business...( after buying Chemicon's arrayer...and taking away > the only real alternative). I have heard from the same reliable source > they may be purchased by a foreign investor...my prayer is that perhaps > that could be Leica! Does anyone have more info on this? I wonder what > this means for those who already have one.....at least I am on the wait > list! We have tried several manual systems from molds to punches....and > there seems no real competition from these, which are difficult to > control or expensive to use. What is the word out there....suggestions > for us "wait-ors"..... CB > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kwuny <@t> email.cs.nsw.gov.au Sun Mar 6 20:20:17 2011 From: kwuny <@t> email.cs.nsw.gov.au (Young Kwun) Date: Sun Mar 6 20:20:28 2011 Subject: [Histonet] immuno stain for nerve in arterial wall In-Reply-To: <594300.54651.qm@web114017.mail.gq1.yahoo.com> References: <594300.54651.qm@web114017.mail.gq1.yahoo.com> Message-ID: <2A4EA853A0DF41BF9F1573CC857117AC@cs.nsw.gov.au> Hi Cindy, You would need to know what kinds of nerves your investigator is interested in. Sympathetic adrenergic nerves travel along arteries and nerves and are found in the adventitia (outer wall of a blood vessel). Varicosities, which are small enlargements along the nerve fibers, are the site of neurotransmitter release. Parasympathetic fibers are also found associated with blood vessels in certain organs such as salivary glands, gastrointestinal glands, and in genital erectile tissues. If interested in both fibers, you could start with antibodies (fluorescent-conjugated) such as Thyrosine hydroxylase, dopamine, serotonin, substance P, Neuropeitide Y, acetylcholine, VIP, CGRP etc., etc.. Cheers, Young Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Repatriation General Hospital Concord NSW 2139 02-9767-6075 (Tel) 02-9767-8427 (Fax) kwuny@email.cs.nsw.gov.au -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C B Sent: Saturday, March 05, 2011 10:06 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] immuno stain for nerve in arterial wall We had an investigator inquire about flourescent immuno stains for?nerve in arterial wall (not sure what species they are working with).? I've never worked with nerve before.? Can someone give me some advice about what primary should I be searching for?? I realize I have to look in to species cross reactivity once I get that information, but I don't even know what to "search" for. Thank you, Cindy Baranowski SJTRI Atlanta, GA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____________________________________________________________________ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From HornHV <@t> archildrens.org Mon Mar 7 13:10:04 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Mar 7 13:10:11 2011 Subject: [Histonet] band saws Message-ID: <25A4DE08332B19499904459F00AAACB7194516A72C@EVS1.archildrens.org> I am looking for a band saw to cut our bone tumors. What do I need to be looking for? Power? Size? Other suggestions? Thanks! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Wanda.Smith <@t> HCAhealthcare.com Mon Mar 7 13:16:09 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Mon Mar 7 13:16:13 2011 Subject: [Histonet] A Spreadsheet for ER/PR Benchmark Comparison Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A0AC32BE@NADCWPMSGCMS03.hca.corpad.net> Good Afternoon All, I am racking my brain trying to figure out how to show the requirement for CAP ANP.22970 patient results of ER/PR/Her2 with published benchmarks. I have collected my data and I have my benchmarks, but I can't seem to wrap my head around how to collate it. Any suggestions or spreadsheets anyone would be willing to share? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From mizlyght <@t> frontier.com Mon Mar 7 13:52:15 2011 From: mizlyght <@t> frontier.com (mizlyght@frontier.com) Date: Mon Mar 7 13:52:19 2011 Subject: [Histonet] DAKO Artisan Kit Issues In-Reply-To: <772445744.1743205.1299527226099.JavaMail.root@cl04-host04.dlls.pa.frontiernet.net> Message-ID: <326758572.1743298.1299527535381.JavaMail.root@cl04-host04.dlls.pa.frontiernet.net> Fellow Histologists: This is my first time using Histonet but I need to ask if anyone has started having problems with their DAKO Artisan kits recently? We have started having leaking kits for Gram Lot # 10043062 and Expiration Date 4/30/2011, and plunger issues with the Iron Lot # 10047616 Expiration Date 10/31/2011. We have also found that the GMS kits Lot # 10049354 and Expiration 11/30/2011 we have received in the last two shipments are from the same lot but they do not work with our validated protocol. What is strange is that the previous GMS kit worked on the same control that we are using now, but does not work with the new kit, but when stained with PAS-Fungus we see the same fungus as we saw with the previous GMS kit. I think it could be something with the shipping method but wanted to see if anyone else has seen a difference. Thanks From flnails <@t> texaschildrens.org Mon Mar 7 14:00:29 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Mon Mar 7 14:00:45 2011 Subject: [Histonet] DAKO Artisan Kit Issues In-Reply-To: <326758572.1743298.1299527535381.JavaMail.root@cl04-host04.dlls.pa.frontiernet.net> References: <772445744.1743205.1299527226099.JavaMail.root@cl04-host04.dlls.pa.frontiernet.net> <326758572.1743298.1299527535381.JavaMail.root@cl04-host04.dlls.pa.frontiernet.net> Message-ID: We have experienced similar issues, however you can just call DAKO and they usually will replace it. It is important that you call them so they will be aware of the problem and can pull that lot # if needed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mizlyght@frontier.com Sent: Monday, March 07, 2011 1:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAKO Artisan Kit Issues Fellow Histologists: This is my first time using Histonet but I need to ask if anyone has started having problems with their DAKO Artisan kits recently? We have started having leaking kits for Gram Lot # 10043062 and Expiration Date 4/30/2011, and plunger issues with the Iron Lot # 10047616 Expiration Date 10/31/2011. We have also found that the GMS kits Lot # 10049354 and Expiration 11/30/2011 we have received in the last two shipments are from the same lot but they do not work with our validated protocol. What is strange is that the previous GMS kit worked on the same control that we are using now, but does not work with the new kit, but when stained with PAS-Fungus we see the same fungus as we saw with the previous GMS kit. I think it could be something with the shipping method but wanted to see if anyone else has seen a difference. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Mar 7 14:20:30 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Mar 7 14:23:43 2011 Subject: [Histonet] RE: A Spreadsheet for ER/PR Benchmark Comparison In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A0AC32BE@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A0AC32BE@NADCWPMSGCMS03.hca.corpad.net> Message-ID: Take your total number of Breast cases and divide by the postitive ER cases to get a percentage of cases. Do the same for the PR and the HER2. 100 breast cases divided by 20 positive ER cases gives you a five percent positive. Your percent positive cases should closely correlate with the national average and published percentages. You could make a pretty graph in excel to go with it, but I don't think that is absolutely necessary. Hope that helps. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com [Wanda.Smith@HCAhealthcare.com] Sent: Monday, March 07, 2011 2:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A Spreadsheet for ER/PR Benchmark Comparison Good Afternoon All, I am racking my brain trying to figure out how to show the requirement for CAP ANP.22970 patient results of ER/PR/Her2 with published benchmarks. I have collected my data and I have my benchmarks, but I can't seem to wrap my head around how to collate it. Any suggestions or spreadsheets anyone would be willing to share? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Mar 7 14:48:35 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 7 14:48:38 2011 Subject: [Histonet] band saws In-Reply-To: <25A4DE08332B19499904459F00AAACB7194516A72C@EVS1.archildrens.org> Message-ID: <221291.75697.qm@web65702.mail.ac4.yahoo.com> Blade speed is more important than size or power. Ren? J. --- On Mon, 3/7/11, Horn, Hazel V wrote: From: Horn, Hazel V Subject: [Histonet] band saws To: "'histonet@lists.utsouthwestern.edu'" Date: Monday, March 7, 2011, 2:10 PM I am looking for a band saw to cut our bone tumors.? What do I need to be looking for?? Power?? Size?? Other suggestions? Thanks! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way? ? Slot 820 Little Rock, AR???72202 phone???501.364.4240 fax? ? ? ? 501.364.3155 visit us on the web at:? ? www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Mon Mar 7 15:31:33 2011 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Mar 7 15:31:58 2011 Subject: [Histonet] band saws In-Reply-To: <221291.75697.qm@web65702.mail.ac4.yahoo.com> References: <221291.75697.qm@web65702.mail.ac4.yahoo.com> Message-ID: <4D754EB5.5030901@pathology.washington.edu> From Tim Taylor of Home Improvement, "You're darn right more power!". Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 3/7/2011 12:48 PM, Rene J Buesa wrote: > Blade speed is more important than size or power. > Ren? J. > > --- On Mon, 3/7/11, Horn, Hazel V wrote: > > > From: Horn, Hazel V > Subject: [Histonet] band saws > To: "'histonet@lists.utsouthwestern.edu'" > Date: Monday, March 7, 2011, 2:10 PM > > > I am looking for a band saw to cut our bone tumors. What do I need to be looking for? Power? Size? Other suggestions? > Thanks! > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Autopsy/Histology/Transcription > Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Mar 7 15:37:23 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Mar 7 15:39:30 2011 Subject: [Histonet] RE: A Spreadsheet for ER/PR Benchmark Comparison In-Reply-To: References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A0AC32BE@NADCWPMSGCMS03.hca.corpad.net>, Message-ID: Sorry folks I meant to say 20% not 5. Use a spread sheet in Excel and a formula Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A [Loralee_Mcmahon@URMC.Rochester.edu] Sent: Monday, March 07, 2011 3:20 PM To: Wanda.Smith@HCAhealthcare.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: A Spreadsheet for ER/PR Benchmark Comparison Take your total number of Breast cases and divide by the postitive ER cases to get a percentage of cases. Do the same for the PR and the HER2. 100 breast cases divided by 20 positive ER cases gives you a five percent positive. Your percent positive cases should closely correlate with the national average and published percentages. You could make a pretty graph in excel to go with it, but I don't think that is absolutely necessary. Hope that helps. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com [Wanda.Smith@HCAhealthcare.com] Sent: Monday, March 07, 2011 2:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A Spreadsheet for ER/PR Benchmark Comparison Good Afternoon All, I am racking my brain trying to figure out how to show the requirement for CAP ANP.22970 patient results of ER/PR/Her2 with published benchmarks. I have collected my data and I have my benchmarks, but I can't seem to wrap my head around how to collate it. Any suggestions or spreadsheets anyone would be willing to share? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From smcbride <@t> andrew.cmu.edu Mon Mar 7 16:40:45 2011 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Mon Mar 7 16:40:51 2011 Subject: [Histonet] band saws In-Reply-To: <25A4DE08332B19499904459F00AAACB7194516A72C@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB7194516A72C@EVS1.archildrens.org> Message-ID: <990F176F-3DEC-442B-B0FF-A915183CDCD4@andrew.cmu.edu> Hi Hazel, In my opinion, you have a few options for saws and blades but it mostly depends upon your budget and where along the process you intend to use the saw (grossing, trimming pmma blocks, cutting sections for ground work, etc.) EXAKT corporation makes an excellent band saw that uses diamond blades, and Well corporation makes a very reputable diamond wire saw. Both of these pieces of equipment are rather expensive, but are capable of precision work that less expensive models cannot match. For less precise work, I have found that Gryphon Corporation (Model C-40) and Mar-Med (Item#80) make a wonderful little diamond band saw that is very useful. A variety of diamond and tooth blade designs are available, and the cost is quite reasonable. I would be more than glad to give you a few pointers off line to share with you my knowledge of what works for me. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu On Mar 7, 2011, at 2:10 PM, Horn, Hazel V wrote: > I am looking for a band saw to cut our bone tumors. What do I need to be looking for? Power? Size? Other suggestions? > Thanks! > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Autopsy/Histology/Transcription > Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From emendez84 <@t> yahoo.com Tue Mar 8 00:27:05 2011 From: emendez84 <@t> yahoo.com (Eric Mendez) Date: Tue Mar 8 00:27:11 2011 Subject: [Histonet] LIS Help Message-ID: <387198.83568.qm@web46314.mail.sp1.yahoo.com> Hi All, I am doing research on different LIS systems and need some guidance. I was wondering if you can send me some information on what everyone uses. I am particularly interested on labs that are devoted solely to GI/small biopsies (i.e esophagus bx, stomach bx, colon bx ..etc) with LIS integration. Has anyone heard of VitalAxis? Psyche Systems? AP Easy? or any other LIS that you have used extensively and would recommend? Any Pro's or Con's to the LIS mentioned above? Do you have a fully integrated barcoding system? What type of volume load annually? Your input would be really appreciated, -E From itai.moshe <@t> mail.huji.ac.il Tue Mar 8 02:36:45 2011 From: itai.moshe <@t> mail.huji.ac.il (Itai Moshe) Date: Tue Mar 8 02:36:58 2011 Subject: [Histonet] Re: Picro sirius red, fast green connective tissue stain In-Reply-To: References: Message-ID: Hi, John The method is a two color staining method that stains collagen in red and muscle fibers in green. The filtration is in order to make the solution that is being used multiple times and stored for a long period, more clear. I've attached an article with an example (Fig2), that is done in the exact way as i described before. http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T9T-4T3KTBS-1&_user=626711&_coverDate=11/30/2008&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_acct=C000032999&_version=1&_urlVersion=0&_userid=626711&md5=805ac6cd7adeb0d8284a07f0be44dcb8&searchtype=a Itai 2011/3/8 John Kiernan > Dear Itai Moshe, > > Can you explain the staining method you described in some Histonet > correspondence nearly a year ago? The correspondence is in the "tail" of > this email. > > Is it published? What is it used for? What is coloured yellow, green or > red? Your procedure stops with a water rinse; are the slides then > dehydrated, cleared and coverslipped? Why does the picric-fast green > solution have to be filtered twice? > > I ask these things because the Biological Stain Commission will soon be > offering certification services for *sirius red F3B* (paper by R. Dapson > et al., *Biotechnic & Histochemistry*, accepted Feb 2011). The BSC testing > includes physico-chemical characterization of the dye, picro-sirius > staining for collagen, and an alkaline SR method for amyloid. Certification > ensures the availability of dye batches that will work properly in their > major applications. > > We are always interested in other uses of biological stains, which might > become more important in the future. > > The method you describe is interesting in that the time in picro-sirius is > very short (5 min). The most thorough mechanistic study of picro-sirius > (Junqueira et al 1979 *Histochem. J.* 11: 447-455) found that the uptake > of the dye by collagen fibres was quite slow, and recommended the > now-standard 60 min. I have not tried the method you describe. I would > expect it to stain the thickest collagen fibres red, erythrocytes yellow, > and everything else, including thin collagen fibres and basement > membranes, yellowy-green. Am I right? > > Any information you can provide about your picro-fast green + picro-sirius > red method will be greatly appreciated, especially the evolution and actual > value of the method. Feel free to share this message with others who use > the method. > > Yours sincerely, > > John Kiernan > > Secretary, Biological Stain Commission > http://biostain.com > J.A.Kiernan MB,ChB,PhD,DSc > Professor, Dept of Anatomy & Cell Biology > University of Western Ontario > London, Canada N6A 5C1 > http://publish.uwo.ca/~jkiernan/index.htm > = = = > ----- Original Message ----- > From: Itai Moshe > Date: Wednesday, April 7, 2010 9:47 > Subject: [Histonet] picro sirius red, sirius red connective tissue > stain[Scanned] > To: histonet@lists.utsouthwestern.edu, baza0013@d.umn.edu, > David.Rushworth@mail.bhrv.nwest.nhs.uk > > > picro Sirius red and Fast green protocol: > > > > Sirius red (Sirius Red F3B)- 0.1% in Picric acid. > > Fast Green - 0.03% in Picric acid (100%). > > Can be re-used, filter twice before each use. > > > > > > 1) Deparaffinization and Rehydration: > > A) Xylen - 10 min X2 > > B) Ethanol 100% - 2 Min X2 > > C) Ethanol 96% - 2 Min X1 > > D) Ethanol 80% - 2 Min X1 > > E) Ethanol 70% - 2 Min X1 > > > > 2) Rinse in double distilled water (DDW) - 5 Min > > > > 3) Rinse in filtered Fast green - 20 Min > > > > 4) Wash with DDW ( just fill the slide box with DDW and empty). > > > > 5) Rinse in filtered Sirius red - 5 Min. > > > > 6) Wash with DDW ( just fill the slides box with DDW and empty). > > > > 7) Check in microscope, if the colors are not strong enough, > > repeat from > > step 2 to 7. > > > > That's it. > > > > -- > > Itai Moshe > > Mark Pines lab > > Institute of Animal Sciences,Volcani Center. > > Dept. of Animal Sciences, School of Veterinary Medicine, The Hebrew > > University of Jerusalem > > Israel > > > > ------Original Message----- > > *Adam Bazama* baza0013 <@t> d.umn.edu > > 40lists.utsouthwestern.edu?Subject=%5BHistonet%5D%20sirius%20red%20connective%20tissue%20stain%5BScanned%5D&In-Reply-To= > > > > *Mon Oct 6 10:27:37 CDT 2008* > > > > > > - Previous message: [Histonet] gastrin > > antibodies.....< > http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-October/039885.html > > > > - Next message: [Histonet] out dated > > reagents< > http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-October/039887.html > > > > - *Messages sorted by:* [ date > > ] > October/date.html#39886> [ thread > > ]< > http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-October/thread.html#39886 > > > > [ subject > > ]< > http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-October/subject.html#39886 > > > > [ author > > ]< > http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-October/author.html#39886 > > > > > > ------------------------------ > > > > Hi Dave, > > > > > > > > I noticed that you posted a request in march of 2004 on histonet > > for a > > Sirius red fast green staining protocol. If you do have a > > protocol, would > > you mind sending it to me. I have had a hard time finding one to > > compare to > > mine and my lack luster results. > > > > > > > > Thank you, > > > > > > > > > > > > Adam Bazama, B.S. > > > > Junior Scientist > > > > Lillehei Heart Institute Histology and Microscopy Core Facility > > > > University of Minnesota Medical School, Division of Cardiology > > 4-266 Nils Hasselmo Hall > > 312 Church Street SE > > Minneapolis, MN 55455 > > Lab Desk: 612-625-6779 > > Cell: 952-334-0607 > > > > -------------------------------------------- > > > > *David Rushworth* David.Rushworth <@t> mail.bhrv.nwest.nhs.uk > > 40lists.utsouthwestern.edu?Subject=%5BHistonet%5D%20sirius%20red%20connective%20tissue%20stain%5BScanned%5D&In-Reply-To= > > > > *Thu Mar 4 09:00:18 CST 2004* > > > > > > - Previous message: [Histonet] reticulin procedure > > > March/004217.html> - Next message: [Histonet] > > Research labs - charges and a question > > about outside contracting > > > March/004219.html> - *Messages sorted by:* [ date ] > > > March/date.html#4218>[ thread ] > > < > http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/thread.html#4218 > > > > [ subject ] > > < > http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/subject.html#4218 > > > > [ author ] > > < > http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/author.html#4218 > > > > > > ------------------------------ > > > > Can someone please supply method for Sirius red-fast green stain for > > connective tissue. > > Thanks in anticipation. > > Dave. > > > > > > > > ------------------------------ > > > > > > - Previous message: [Histonet] reticulin > > procedure< > http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/004217.html > > > > - Next message: [Histonet] Research labs - charges > > and a question about > > outside > > contracting< > http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/004219.html > > > > - *Messages sorted by:* [ date > > ] > March/date.html#4218> [ thread > > ]< > http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/thread.html#4218 > > > > [ subject > > ]< > http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/subject.html#4218 > > > > [ author > > ]< > http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/author.html#4218 > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Itai Moshe Mark Pines lab Institute of Animal Sciences, Volcani Center. Dept. of Animal Sciences, School of Veterinary Medicine, The Hebrew University of Jerusalem Israel From jqb7 <@t> cdc.gov Tue Mar 8 07:20:16 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Mar 8 07:20:24 2011 Subject: [Histonet] microtome safety Message-ID: Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From micropathlabs <@t> yahoo.com Tue Mar 8 07:26:22 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Tue Mar 8 07:26:28 2011 Subject: [Histonet] microtome safety In-Reply-To: References: Message-ID: <741760.69697.qm@web161716.mail.bf1.yahoo.com> Every tech in my facility uses something different.?Some use fingers, some use forceps (usually curved), one uses?a teasing needle and one uses a paint brush. It's up to the individual's technique but we do try to discourage?using one's fingers too close to the blade. Hope this?helps. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ? ________________________________ From: "Bartlett, Jeanine (CDC/OID/NCEZID)" To: "histonet@lists.utsouthwestern.edu" Sent: Tue, March 8, 2011 8:20:16 AM Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question:? do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning?? For those that do not use their fingers, what do you use?? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA? 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Tue Mar 8 07:29:52 2011 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Tue Mar 8 07:29:59 2011 Subject: [Histonet] Measure productivity by skill level Message-ID: <18984.203.135.35.66.1299590992.squirrel@brain.net.pk> Dear Fellow Histology Professionals, My senior manager give me a task to present a presentation on how to measure and calculate the work lode for manpower (productivity by skill level), we already use Welcan units. Does anyone know who can I prepare? Your input would be really appreciated, Muhammad Tahseen Supervisor Histology SKMCH&RC Lahore Pakistan From trathborne <@t> somerset-healthcare.com Tue Mar 8 07:33:54 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Mar 8 07:35:05 2011 Subject: [Histonet] microtome safety In-Reply-To: <741760.69697.qm@web161716.mail.bf1.yahoo.com> Message-ID: Same here. One tech keeps her index fingernail extra long for this purpose. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sheila Haas Sent: Tuesday, March 08, 2011 8:26 AM To: Bartlett, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] microtome safety Every tech in my facility uses something different.?Some use fingers, some use forceps (usually curved), one uses?a teasing needle and one uses a paint brush. It's up to the individual's technique but we do try to discourage?using one's fingers too close to the blade. Hope this?helps. ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ? ________________________________ From: "Bartlett, Jeanine (CDC/OID/NCEZID)" To: "histonet@lists.utsouthwestern.edu" Sent: Tue, March 8, 2011 8:20:16 AM Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question:? do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning?? For those that do not use their fingers, what do you use?? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA? 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From LSebree <@t> uwhealth.org Tue Mar 8 07:37:11 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Mar 8 07:37:16 2011 Subject: [Histonet] microtome safety In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E273801002959@UWHC-MAIL01.uwhis.hosp.wisc.edu> I've always used my fingers but some others in my lab use forceps. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 08, 2011 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 8 07:53:33 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 8 07:53:36 2011 Subject: [Histonet] microtome safety In-Reply-To: Message-ID: <551130.8098.qm@web65703.mail.ac4.yahoo.com> I always found better and used a wet?"camel's" hair pencil. It provides the most gentle pull on the sections. Ren? J. ? ? --- On Tue, 3/8/11, Bartlett, Jeanine (CDC/OID/NCEZID) wrote: From: Bartlett, Jeanine (CDC/OID/NCEZID) Subject: [Histonet] microtome safety To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 8, 2011, 8:20 AM Morning all! I need some quick responses to this question:? do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning?? For those that do not use their fingers, what do you use?? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA? 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Mar 8 07:56:54 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Mar 8 07:57:00 2011 Subject: [Histonet] RE: microtome safety Message-ID: Thanks everyone, I think I have enough information. I really appreciate all the replies! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _____________________________________________ From: Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 08, 2011 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From asmith <@t> mail.barry.edu Tue Mar 8 07:59:49 2011 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Mar 8 07:59:54 2011 Subject: [Histonet] RE: microtome safety In-Reply-To: References: Message-ID: My mentor used forceps. Thus I have always used forceps. I think the paraffin ribbon would melt onto fingers on a warm day. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 08, 2011 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Mar 8 08:15:35 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Mar 8 08:15:42 2011 Subject: [Histonet] microtome safety In-Reply-To: <551130.8098.qm@web65703.mail.ac4.yahoo.com> References: <551130.8098.qm@web65703.mail.ac4.yahoo.com> Message-ID: <7E20296BF73643F2958B326AE5C096AA@prueggihctechlt> Jeanine, I use forceps with the tips bent at a 90 degree angle for reaching under the sections to remove air bubbles. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 08, 2011 6:54 AM To: histonet@lists.utsouthwestern.edu; Jeanine (CDC/OID/NCEZID)Bartlett Subject: Re: [Histonet] microtome safety I always found better and used a wet?"camel's" hair pencil. It provides the most gentle pull on the sections. Ren? J. ? ? --- On Tue, 3/8/11, Bartlett, Jeanine (CDC/OID/NCEZID) wrote: From: Bartlett, Jeanine (CDC/OID/NCEZID) Subject: [Histonet] microtome safety To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 8, 2011, 8:20 AM Morning all! I need some quick responses to this question:? do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning?? For those that do not use their fingers, what do you use?? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA? 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Mar 8 08:19:30 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Mar 8 08:19:35 2011 Subject: [Histonet] microtome safety References: <551130.8098.qm@web65703.mail.ac4.yahoo.com> Message-ID: <584D693088C34B1E86D5063C41925BDF@prueggihctechlt> Actually I need to qualify my answer, I use my fingers to grab end of the ribbon the farest from the blade and curved forceps near the blade so I use both finger and forceps at the same time. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Tuesday, March 08, 2011 7:16 AM To: 'Rene J Buesa'; 'histonet@lists.utsouthwestern.edu'; 'Jeanine (CDC/OID/NCEZID)Bartlett' Subject: RE: [Histonet] microtome safety Jeanine, I use forceps with the tips bent at a 90 degree angle for reaching under the sections to remove air bubbles. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 08, 2011 6:54 AM To: histonet@lists.utsouthwestern.edu; Jeanine (CDC/OID/NCEZID)Bartlett Subject: Re: [Histonet] microtome safety I always found better and used a wet?"camel's" hair pencil. It provides the most gentle pull on the sections. Ren? J. ? ? --- On Tue, 3/8/11, Bartlett, Jeanine (CDC/OID/NCEZID) wrote: From: Bartlett, Jeanine (CDC/OID/NCEZID) Subject: [Histonet] microtome safety To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 8, 2011, 8:20 AM Morning all! I need some quick responses to this question:? do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning?? For those that do not use their fingers, what do you use?? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA? 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgrobert <@t> rci.rutgers.edu Tue Mar 8 08:36:47 2011 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Tue Mar 8 08:36:51 2011 Subject: [Histonet] microtome safety In-Reply-To: References: Message-ID: I use forceps, the thinner the tips, the better. I've had people in here who use brushes, too. I can't use my fingers, the ribbons always stick. Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 > Same here. One tech keeps her index fingernail extra long for this > purpose. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sheila > Haas > Sent: Tuesday, March 08, 2011 8:26 AM > To: Bartlett, Jeanine (CDC/OID/NCEZID); > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] microtome safety > > > Every tech in my facility uses something different.??Some use fingers, > some use > forceps (usually curved), one uses??a teasing needle and one uses a paint > brush. > It's up to the individual's technique but we do try to discourage??using > one's > fingers too close to the blade. > Hope this??helps. > ?? > Sheila Haas > Laboratory Supervisor > MicroPath Laboratories, Inc. > ?? > > > > > ________________________________ > From: "Bartlett, Jeanine (CDC/OID/NCEZID)" > To: "histonet@lists.utsouthwestern.edu" > > Sent: Tue, March 8, 2011 8:20:16 AM > Subject: [Histonet] microtome safety > > Morning all! > > I need some quick responses to this question:?? do you use your fingers or > an > instrument of some sort to pull your paraffin ribbons off the block when > sectioning??? For those that do not use their fingers, what do you use??? > If > forceps, are these the typical lab forceps or a special type? > > Thanks so much! > > Jeanine Bartlett, BS, HT(ASCP)QIHC > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA?? 30333 > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in > this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sgoebel <@t> mirnarx.com Tue Mar 8 08:40:55 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Tue Mar 8 08:40:58 2011 Subject: [Histonet] microtome safety In-Reply-To: References: Message-ID: I use the end (without bristles) of a paintbrush Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 08, 2011 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue Mar 8 08:42:10 2011 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Tue Mar 8 08:42:41 2011 Subject: [Histonet] microtome safety In-Reply-To: <7E20296BF73643F2958B326AE5C096AA@prueggihctechlt> References: <551130.8098.qm@web65703.mail.ac4.yahoo.com> <7E20296BF73643F2958B326AE5C096AA@prueggihctechlt> Message-ID: <7722595275A4DD4FA225B92CDBF174A1012CD3C25407@EXC-MBX3.cfs.le.ac.uk> Well 30 years ago I held the sections between thumb and index finger of my left hand, then after 10 years it was my thumb and middle finger, and then followed by my thumb and ring finger, recently I have had to start using my right hand but have been promised a pair of forceps. Cheers Richard "Knuckles" Edwards -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: 08 March 2011 14:16 To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; 'Jeanine (CDC/OID/NCEZID)Bartlett' Subject: RE: [Histonet] microtome safety Jeanine, I use forceps with the tips bent at a 90 degree angle for reaching under the sections to remove air bubbles. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 08, 2011 6:54 AM To: histonet@lists.utsouthwestern.edu; Jeanine (CDC/OID/NCEZID)Bartlett Subject: Re: [Histonet] microtome safety I always found better and used a wet?"camel's" hair pencil. It provides the most gentle pull on the sections. Ren? J. ? ? --- On Tue, 3/8/11, Bartlett, Jeanine (CDC/OID/NCEZID) wrote: From: Bartlett, Jeanine (CDC/OID/NCEZID) Subject: [Histonet] microtome safety To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 8, 2011, 8:20 AM Morning all! I need some quick responses to this question:? do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning?? For those that do not use their fingers, what do you use?? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA? 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Tue Mar 8 08:48:01 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Mar 8 08:48:05 2011 Subject: [Histonet] Re: band saws Message-ID: Hazel Horn in Little Rock AR asks: I am looking for a band saw to cut our bone tumors. What do I need to be looking for? Power? Size? Other suggestions? I don't have any personal experience with it, but at a program about orthopedic pathology about 10 years ago I heard about a hardware-store item called a scroll saw. It was cheap - about $200 for quality, $100 for Chinese - and said to be just impossible to cut yourself with. Disadvantage was its fairly large tabletop footprint. The Cowboy Way for pathologists sawing bone is a Civil War era amputation saw, called a Satterlee saw. (I actually saw one in a Civil War re-enactors' field hospital, complete with chrome plating.) They last a long time. If I'm not allowed a Satterlee saw, I go to a hardware store and buy a hacksaw. (Many small pathology services are no longer able to cut bone.) Bob Richmond Samurai Pathologist / Old Sawbones Knoxville TN From TMcNemar <@t> lmhealth.org Tue Mar 8 08:49:59 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Mar 8 08:50:02 2011 Subject: [Histonet] RE: microtome safety In-Reply-To: References: Message-ID: Both. Fingers to lift one end of the ribbon as I cut it and then with the other hand I use a small brush to lift the other end from the microtome. Allows me to stretch and smooth the ribbon as I lay it out on the water bath. Some others here us forceps, picks, etc. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 08, 2011 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From mhale <@t> carisls.com Tue Mar 8 08:51:02 2011 From: mhale <@t> carisls.com (Hale, Meredith) Date: Tue Mar 8 08:51:07 2011 Subject: [Histonet] HT Position New Mexico Message-ID: <6F33D8418806044682A391273399860F073B1403@s-irv-ex301.PathologyPartners.intranet> know what else I can assist you with . Thanks Great opportunity for a Histotechnician in a brand new laboratory! Digestive Disease Consultants of Las Cruces , NM is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part time position that offers a competitive salary and the flexible hours allow you to put your own personal stamp on the laboratory. Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mhale@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mhale@carisls.com From HornHV <@t> archildrens.org Tue Mar 8 08:57:31 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Mar 8 08:57:36 2011 Subject: [Histonet] RE: microtome safety In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB7194516A732@EVS1.archildrens.org> I do the same as Tom. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, March 08, 2011 8:50 AM To: 'Bartlett, Jeanine (CDC/OID/NCEZID)'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: microtome safety Both. Fingers to lift one end of the ribbon as I cut it and then with the other hand I use a small brush to lift the other end from the microtome. Allows me to stretch and smooth the ribbon as I lay it out on the water bath. Some others here us forceps, picks, etc. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 08, 2011 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From sgoebel <@t> mirnarx.com Tue Mar 8 09:06:02 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Tue Mar 8 09:06:06 2011 Subject: [Histonet] cell blocks Message-ID: So...I am trying to make a cell block using 0.9% agar. I have done this in the past with no problems. It was at a different facility and I don't know if the agar is the same? I used bacto-agar this time, but last week someone tried to do my protocol with agarose and the "boogers" dried up and fell out of the block. The only other difference is we have clear rite xylene substitute in this processor where the other one I used had xylene. Tissues processed on the same cycle come out fine with the clear rite so I don't think that it the problem. When the cell blocks come out they are as thin as the papers I wrapped them in making it almost impossible to see and to subsequently cut. Any suggestions for making cell blocks without using the plasma/thrombin method or the insanely expensive histo-gel would be appreciated. Second, does anyone have a suggestion on how to repair the cells that are embedded in the tissue paper thick "cell block"? Thanks guys and gals! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From Bauer.Karen <@t> mayo.edu Tue Mar 8 09:16:50 2011 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Tue Mar 8 09:16:55 2011 Subject: [Histonet] Controls for IPs Message-ID: <53FC421CC200C5429929EDE6C3676F30014EC205@msgebe34> Hello, It seems we go back and forth between multi-tissue control blocks and single tissue control blocks when it comes to immunostaining. In the past we used single blocks and still continue to use some for the IPs that are rarely used. Then, switched to creating multi-tissue blocks for ease of use. It's so nice to cut control slides ahead of time and just pull them out and add our patient tissue. But... we find that the multi-tissue blocks are using up hard to find tissue. For example, we have a melanoma skin in one of the MTBs for the HMB45 and Melan A stains, but that MTB is used more for the CK7, CK20 and TTF-1 stains. Basically, that melanoma skin is just being wasted. I'm considering going back to the single block controls and was wondering what other labs are doing. What is the concensus in HistoLand in regards to IP control blocks/tissues? Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System bauer.karen@mayo.edu From MSHERWOOD <@t> PARTNERS.ORG Tue Mar 8 09:19:41 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Mar 8 09:19:47 2011 Subject: [Histonet] microtome safety In-Reply-To: References: Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5520@PHSXMB30.partners.org> Just regular forceps. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 08, 2011 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From sfeher <@t> CMC-NH.ORG Tue Mar 8 09:21:43 2011 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Tue Mar 8 09:21:45 2011 Subject: [Histonet] Alcohol & Xylene recyclers In-Reply-To: <765336.52766.qm@web113811.mail.gq1.yahoo.com> References: <765336.52766.qm@web113811.mail.gq1.yahoo.com> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC24669BBE@exchange.cmc-nh.org> We use Creative Waste Solutions recyclers. We have alcohol and formalin recyclers and utilize xylene absorption pads to get he water out of the xylene and reuse it. We choose these recyclers because they are a gravity feed system that does not require a power source, they are quiet so we can locate them adjacent the materials we want to recycle, thus adding to the LEAN aspect of these units. We found that these were very cost effective as well when considered alongside the other recyclers that are available. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, March 02, 2011 1:42 PM To: histonet Subject: [Histonet] Alcohol & Xylene recyclers Hi Everyone in histoland! I would like to get your feedback on which alcohol / xylene recyling units you prefer.? I would like?information regarding?purity of end product, cost, size of footprint, and relyability.? We currently do not have a recycling unit, and I have been requested to gather information. ? Thank you, Akemi Allison BS, HT(ASCP)HTL E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.Fleming <@t> allina.com Tue Mar 8 09:25:06 2011 From: Jackie.Fleming <@t> allina.com (Fleming, Jackie M) Date: Tue Mar 8 09:25:11 2011 Subject: [Histonet] Alcohol & Xylene recyclers In-Reply-To: <0908FC0A43B87A4FB60EDCCA06AABC24669BBE@exchange.cmc-nh.org> References: <765336.52766.qm@web113811.mail.gq1.yahoo.com>, <0908FC0A43B87A4FB60EDCCA06AABC24669BBE@exchange.cmc-nh.org> Message-ID: <10F1C4C3-2CEC-4643-9931-25CD84370566@mimectl> The CWS recyclers didn't work for us because of volume and too many bloody specimens.We have to use the B/R recyclers. Jackie Fleming HT ASCP Technical Consultant - Histology Phone: 612-863- 4773 Pager: 612-654-2135 e-mail: jackie.fleming@allina.com From: Feher, Stephen Sent: Tue 3/8/2011 9:21 AM To: Akemi Allison; histonet Subject: RE: [Histonet] Alcohol & Xylene recyclers We use Creative Waste Solutions recyclers. We have alcohol and formalin recyclers and utilize xylene absorption pads to get he water out of the xylene and reuse it. We choose these recyclers because they are a gravity feed system that does not require a power source, they are quiet so we can locate them adjacent the materials we want to recycle, thus adding to the LEAN aspect of these units. We found that these were very cost effective as well when considered alongside the other recyclers that are available. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, March 02, 2011 1:42 PM To: histonet Subject: [Histonet] Alcohol & Xylene recyclers Hi Everyone in histoland! I would like to get your feedback on which alcohol / xylene recyling units you prefer. I would like information regarding purity of end product, cost, size of footprint, and relyability. We currently do not have a recycling unit, and I have been requested to gather information. Thank you, Akemi Allison BS, HT(ASCP)HTL E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. From AlieteE_Langsdorf <@t> DFCI.HARVARD.EDU Tue Mar 8 09:59:46 2011 From: AlieteE_Langsdorf <@t> DFCI.HARVARD.EDU (Langsdorf, Aliete E.) Date: Tue Mar 8 09:59:52 2011 Subject: [Histonet] ArrayMold System Message-ID: <064301362827D243B0F0D3B63E70906305644C44@PHSXMB24.partners.org> Hi, I am also interested in hearing about the ArrayMold vs Beecher vs any other do-it-yourself TMA systems. Any favorites systems/recommendations or suggestions? Thanks ~Ally Senior Research Technician Medical Oncology Dana-Farber Cancer Institute re: Wondering if anyone has used/purchased the ArrayMold "tissue micro array system"?? Positive and negative feedback would be greatly appreciated :) Nancy Heath, HT(ASCP) March 10, 2011 is Histotechnology Professionals Day Senior Research Technician Medical Oncology Dana-Farber Cancer Institute The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Laura.Miller <@t> leica-microsystems.com Tue Mar 8 10:01:33 2011 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Tue Mar 8 10:01:41 2011 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 03/06/2011 and will not return until 03/14/2011. I will be out of the country until Monday, March 14th. While I will be checking messages, my response time will be delayed. Thank you ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From nto <@t> stowers.org Tue Mar 8 10:16:46 2011 From: nto <@t> stowers.org (Thomas, Nancy) Date: Tue Mar 8 10:16:53 2011 Subject: [Histonet] RE: cell blocks In-Reply-To: Message-ID: Sarah, The clear rite is the problem. We have it on our processor for routine paraffin processing and it works well. But if we use histogel or agarose, we make sure to use pure xylene ( not even recycled xylene). In past experiments, we have found that in the clear rite station the gel hardens, shrinks and discolors. Nancy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Tuesday, March 08, 2011 9:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell blocks So...I am trying to make a cell block using 0.9% agar. I have done this in the past with no problems. It was at a different facility and I don't know if the agar is the same? I used bacto-agar this time, but last week someone tried to do my protocol with agarose and the "boogers" dried up and fell out of the block. The only other difference is we have clear rite xylene substitute in this processor where the other one I used had xylene. Tissues processed on the same cycle come out fine with the clear rite so I don't think that it the problem. When the cell blocks come out they are as thin as the papers I wrapped them in making it almost impossible to see and to subsequently cut. Any suggestions for making cell blocks without using the plasma/thrombin method or the insanely expensive histo-gel would be appreciated. Second, does anyone have a suggestion on how to repair the cells that are embedded in the tissue paper thick "cell block"? Thanks guys and gals! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Tue Mar 8 10:32:18 2011 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Mar 8 10:32:25 2011 Subject: [Histonet] Herpes Virus In-Reply-To: References: Message-ID: All, I am looking for a good HSVI/HSVII cocktail for IHC. Can anyone out there suggest a good one? Thank-you in Advance, Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI From JWeems <@t> sjha.org Tue Mar 8 10:37:12 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Mar 8 10:37:16 2011 Subject: [Histonet] RE: Herpes Virus In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081E1BE12E@CHEXCMS10.one.ads.che.org> We use Cell Marque RTU 361A-18 362A-18 And mix them together to make our own. Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, March 08, 2011 11:32 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Herpes Virus All, I am looking for a good HSVI/HSVII cocktail for IHC. Can anyone out there suggest a good one? Thank-you in Advance, Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From AlieteE_Langsdorf <@t> DFCI.HARVARD.EDU Tue Mar 8 10:37:56 2011 From: AlieteE_Langsdorf <@t> DFCI.HARVARD.EDU (Langsdorf, Aliete E.) Date: Tue Mar 8 10:38:02 2011 Subject: [Histonet] Preferred green counterstain for GMS? Message-ID: <064301362827D243B0F0D3B63E70906305644C46@PHSXMB24.partners.org> Hi, What is your preferred green counterstain for GMS? In the kit I have, it suggests Fast Green FCF, Light Green SF Yellowish or Tartrazine. Which should I buy? Any help is appreciated! Thanks! ~Ally Senior Research Technician Medical Oncology Dana-Farber Cancer Institute The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From sfonner <@t> labpath.com Tue Mar 8 10:38:03 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Tue Mar 8 10:39:53 2011 Subject: [Histonet] Herpes Virus In-Reply-To: References: Message-ID: <001601cbddaf$31a67940$94f36bc0$@com> I am really interested in this also. And in addition to the Simplex I and II, does anyone know of a good antibody for Zoster (Varicella)? Thanks, Sheila Fonner HT (ASCP) KDL IHC Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, March 08, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Herpes Virus All, I am looking for a good HSVI/HSVII cocktail for IHC. Can anyone out there suggest a good one? Thank-you in Advance, Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Mar 8 10:51:57 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Mar 8 10:52:08 2011 Subject: [Histonet] microtome safety In-Reply-To: Message-ID: I use curved forceps to hold the ribbon and a paint brush to release it from the knife edge. The curved forcepts help to separate the sections and remove air bubbles. Jennifer MacDonald "Bartlett, Jeanine (CDC/OID/NCEZID)" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/08/2011 05:25 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Mar 8 10:52:29 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Mar 8 10:52:34 2011 Subject: [Histonet] Herpes Virus In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E273801002964@UWHC-MAIL01.uwhis.hosp.wisc.edu> We get the single Abs from Cell Marque, polyclonals, and cocktail them ourselves. I learned the hard way that vendors are no longer able to sell these already cocktailed. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, March 08, 2011 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Herpes Virus All, I am looking for a good HSVI/HSVII cocktail for IHC. Can anyone out there suggest a good one? Thank-you in Advance, Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pete.Pedersen <@t> HealthONEcares.com Tue Mar 8 11:39:45 2011 From: Pete.Pedersen <@t> HealthONEcares.com (Pete.Pedersen@HealthONEcares.com) Date: Tue Mar 8 11:52:28 2011 Subject: [Histonet] Testing for Chemo Sensitivity Message-ID: Hello all! I was wondering if anyone has recently sent out a specimen for chemo sensitivity. If so where? Does the specimen need to be fresh/fixed? Is there a kit? Thanks in advance for your responses!!! PETE From foreightl <@t> gmail.com Tue Mar 8 12:52:39 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Mar 8 12:52:43 2011 Subject: [Histonet] Preferred green counterstain for GMS? In-Reply-To: <064301362827D243B0F0D3B63E70906305644C46@PHSXMB24.partners.org> References: <064301362827D243B0F0D3B63E70906305644C46@PHSXMB24.partners.org> Message-ID: Our lab uses Light Green SF yellowish. Both Carson and Shehan's procedure (adapted from AFIP) use Light Green SF yellowish. However either Light Green SF yellowish or Fast Green FCF imparts a green color to all components of the section (J.A. Kiernan). Tartrazine is yellow. Good luck! On Tue, Mar 8, 2011 at 8:37 AM, Langsdorf, Aliete E. wrote: > Hi, > > What is your preferred green counterstain for GMS? > In the kit I have, it suggests Fast Green FCF, Light Green SF Yellowish or > Tartrazine. > Which should I buy? > > Any help is appreciated! > Thanks! > ~Ally > > Senior Research Technician > Medical Oncology > Dana-Farber Cancer Institute > > > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From TNMayer <@t> mdanderson.org Tue Mar 8 12:53:07 2011 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue Mar 8 12:53:12 2011 Subject: [Histonet] RE: Histonet Digest, Vol 88, Issue 10 microtome safety In-Reply-To: <9f6041ee-b378-4db9-bffb-f93a600d5fde@DCPWPRTR01.mdanderson.edu> References: <9f6041ee-b378-4db9-bffb-f93a600d5fde@DCPWPRTR01.mdanderson.edu> Message-ID: I use anything except for a wooden pick. The pick is hard to use. I prefer the curved forceps and a brush, but depending on what is readily available a teasing needle or my'fingernail'will have to do. I try not use my nail, due to the safety risk, but in a pinch it works. Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org "Bartlett, Jeanine (CDC/OID/NCEZID)" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/08/2011 05:25 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From dgoodwin <@t> rwjuhh.edu Tue Mar 8 13:07:19 2011 From: dgoodwin <@t> rwjuhh.edu (Goodwin, Diana) Date: Tue Mar 8 13:07:24 2011 Subject: [Histonet] Re: Bone marrow smear fixation Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71E70A6D8FF@HAMEXMBA.rwjham.local> We are air-drying our smears. I have worked other places that fixed them for 5 min. in Methanol. Maybe do both and compare the morphology. Diana G. Goodwin, BS, HT(ASCP)QIHC Department of Pathology Robert Wood Johnson University Hospital at Hamilton Hamilton, NJ 08610 609-631-6996 dgoodwin@rwjuhh.edu From macveigh <@t> usc.edu Tue Mar 8 14:01:21 2011 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Tue Mar 8 14:01:17 2011 Subject: [Histonet] Measure productivity by skill level Message-ID: <003c01cbddcb$98a6f810$c9f4e830$@usc.edu> I would also be interested to know that. I am not even familiar with Welcan units. Would someone please elaborate? Michelle Aloni From Norm.Burnham <@t> propath.com Tue Mar 8 14:06:52 2011 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Tue Mar 8 14:06:58 2011 Subject: [Histonet] Measure productivity by skill level Message-ID: <82C7248978CB50469FD6BA68EBBEFE670397551B@exchange.propathlab.com> Try googling it. ----- Original Message ----- From: histonet-bounces@lists.utsouthwestern.edu To: Histonet@lists.utsouthwestern.edu Sent: Tue Mar 08 14:01:21 2011 Subject: [Histonet] Measure productivity by skill level I would also be interested to know that. I am not even familiar with Welcan units. Would someone please elaborate? Michelle Aloni _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From macveigh <@t> usc.edu Tue Mar 8 14:16:38 2011 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Tue Mar 8 14:16:32 2011 Subject: [Histonet] Measure productivity by skill level Message-ID: <004901cbddcd$bacecba0$306c62e0$@usc.edu> THANKS! It worked J Michelle From Christina.Wilson <@t> leica-microsystems.com Tue Mar 8 15:31:26 2011 From: Christina.Wilson <@t> leica-microsystems.com (Christina.Wilson@leica-microsystems.com) Date: Tue Mar 8 15:31:36 2011 Subject: [Histonet] AUTO: is out of the office. (returning Thu 03/17/2011) Message-ID: I am out of the office from Tue 03/08/2011 until Thu 03/17/2011. I will have limited access to emails during this time. If you should need assistance, please contact Demaris Mills, demaris.mills@leica-microsystems.com, for product management support or Karen Niewerth, karen.niewerth@leica-microsystems.com, for customer service support. Note: This is an automated response to your message "Histonet Digest, Vol 88, Issue 10" sent on 3/8/2011 11:53:14 AM. This is the only notification you will receive while this person is away. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From laurie.reilly <@t> jcu.edu.au Tue Mar 8 16:21:39 2011 From: laurie.reilly <@t> jcu.edu.au (Reilly, Laurie) Date: Tue Mar 8 16:22:08 2011 Subject: [Histonet] RE: Preferred green counterstain for GMS? In-Reply-To: <064301362827D243B0F0D3B63E70906305644C46@PHSXMB24.partners.org> References: <064301362827D243B0F0D3B63E70906305644C46@PHSXMB24.partners.org> Message-ID: My mentors way back taught us to use Methyl Green as a counterstain for GMS because it also stains nuclei, but it has to be cleared with chloroform to remove Methyl Violet contamination. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Langsdorf, Aliete E. Sent: Wednesday, 9 March 2011 2:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Preferred green counterstain for GMS? Hi, What is your preferred green counterstain for GMS? In the kit I have, it suggests Fast Green FCF, Light Green SF Yellowish or Tartrazine. Which should I buy? Any help is appreciated! Thanks! ~Ally Senior Research Technician Medical Oncology Dana-Farber Cancer Institute The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abeharry798 <@t> gmail.com Tue Mar 8 18:09:31 2011 From: abeharry798 <@t> gmail.com (Andrea) Date: Tue Mar 8 18:07:10 2011 Subject: [Histonet] Computerized inventory system Message-ID: <55D0669D-7A4A-4FD2-A38F-69BBB47FF2DF@gmail.com> Hi everyone, Just wondering if anyone is using or heard of any lab inventory system on the market. We have been looking at a system called Cove.Can anyone give me any feedback Thanks, Andrea Beharry Technical Specialist- Pathology William Osler Health System From AnthonyH <@t> chw.edu.au Tue Mar 8 18:42:36 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Mar 8 18:42:48 2011 Subject: [Histonet] RE: cell blocks In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715718832726@xmdb02.nch.kids> Sarah, It is important that the agar cell block is allowed to fix in formalin (10%NBF) before wrapping in paper, at least 2 hours. It will hold its shape better. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Wednesday, 9 March 2011 2:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell blocks So...I am trying to make a cell block using 0.9% agar. I have done this in the past with no problems. It was at a different facility and I don't know if the agar is the same? I used bacto-agar this time, but last week someone tried to do my protocol with agarose and the "boogers" dried up and fell out of the block. The only other difference is we have clear rite xylene substitute in this processor where the other one I used had xylene. Tissues processed on the same cycle come out fine with the clear rite so I don't think that it the problem. When the cell blocks come out they are as thin as the papers I wrapped them in making it almost impossible to see and to subsequently cut. Any suggestions for making cell blocks without using the plasma/thrombin method or the insanely expensive histo-gel would be appreciated. Second, does anyone have a suggestion on how to repair the cells that are embedded in the tissue paper thick "cell block"? Thanks guys and gals! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From AnthonyH <@t> chw.edu.au Tue Mar 8 18:48:12 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Mar 8 18:48:24 2011 Subject: [Histonet] RE: Preferred green counterstain for GMS? In-Reply-To: <064301362827D243B0F0D3B63E70906305644C46@PHSXMB24.partners.org> References: <064301362827D243B0F0D3B63E70906305644C46@PHSXMB24.partners.org> Message-ID: <6D6BD1DE8A5571489398B392A38A715718832752@xmdb02.nch.kids> Ally, I would use Fast Green FCF. It does not fade as much as Light Green. Use a 1% in 2% Acetic acid. Tartrazine will give you a yellow colour. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Langsdorf, Aliete E. Sent: Wednesday, 9 March 2011 3:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Preferred green counterstain for GMS? Hi, What is your preferred green counterstain for GMS? In the kit I have, it suggests Fast Green FCF, Light Green SF Yellowish or Tartrazine. Which should I buy? Any help is appreciated! Thanks! ~Ally Senior Research Technician Medical Oncology Dana-Farber Cancer Institute The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From amitapandey <@t> torrentpharma.com Wed Mar 9 01:26:58 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Wed Mar 9 01:30:20 2011 Subject: [Histonet] microtome safety In-Reply-To: References: Message-ID: We use fingers...I am not aware of any kind of forceps. But along with you i am also curious to know if any thing else is available. Amita From: "Bartlett, Jeanine (CDC/OID/NCEZID)" To: "histonet@lists.utsouthwestern.edu" Date: 08/03/11 06:52 PM Subject: [Histonet] microtome safety Sent by: histonet-bounces@lists.utsouthwestern.edu Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From funderwood <@t> mcohio.org Wed Mar 9 07:26:43 2011 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Wed Mar 9 07:27:26 2011 Subject: [Histonet] ExCell Plus fixative Message-ID: <4D7738A1.BE64.0034.1@mcohio.org> Hello Histo Land, Does anyone out there have experience using ExCell Plus formalin substitute? Thanks in advance From rfields <@t> gidocs.net Wed Mar 9 07:35:18 2011 From: rfields <@t> gidocs.net (Rosa Fields) Date: Wed Mar 9 07:37:11 2011 Subject: [Histonet] barcoding.. References: <6D6BD1DE8A5571489398B392A38A715718832726@xmdb02.nch.kids> Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F0160E342@GIEXCHANGE.gidocs.net> Anyone using TBS's SHUR/Mark? Software? Looking for references, what works, downtime, what would you change if you could? Thanks! Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? From dmccaig <@t> ckha.on.ca Wed Mar 9 08:15:48 2011 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Wed Mar 9 08:15:56 2011 Subject: [Histonet] diastase Message-ID: I am looking to purchase diastase to use for the PAS/DIASTASE stain. I see there are many options in the catalogues. What is recommended? diana From mpence <@t> grhs.net Wed Mar 9 08:28:54 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Mar 9 08:28:59 2011 Subject: [Histonet] diastase In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974B71@is-e2k3.grhs.net> I have a long distance on-line histology student in my lab right now getting her practical experience. She was doing special stains this past week and had PAS with and without diastase. I had her do the stains with both commercially available diastase and also using saliva so she could see the difference. Nothing like the stains we use to do in the "Good Ol' Days"! What a difference. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, March 09, 2011 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] diastase I am looking to purchase diastase to use for the PAS/DIASTASE stain. I see there are many options in the catalogues. What is recommended? diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Wed Mar 9 08:52:57 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Mar 9 08:53:03 2011 Subject: [Histonet] RE: cell blocks In-Reply-To: <6D6BD1DE8A5571489398B392A38A715718832726@xmdb02.nch.kids> References: <6D6BD1DE8A5571489398B392A38A715718832726@xmdb02.nch.kids> Message-ID: I actually fixed the cells without the agar overnight, and left them in the agar overnight... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: Tuesday, March 08, 2011 6:43 PM To: Sarah Goebel; histonet@lists.utsouthwestern.edu Subject: RE: cell blocks Sarah, It is important that the agar cell block is allowed to fix in formalin (10%NBF) before wrapping in paper, at least 2 hours. It will hold its shape better. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Wednesday, 9 March 2011 2:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell blocks So...I am trying to make a cell block using 0.9% agar. I have done this in the past with no problems. It was at a different facility and I don't know if the agar is the same? I used bacto-agar this time, but last week someone tried to do my protocol with agarose and the "boogers" dried up and fell out of the block. The only other difference is we have clear rite xylene substitute in this processor where the other one I used had xylene. Tissues processed on the same cycle come out fine with the clear rite so I don't think that it the problem. When the cell blocks come out they are as thin as the papers I wrapped them in making it almost impossible to see and to subsequently cut. Any suggestions for making cell blocks without using the plasma/thrombin method or the insanely expensive histo-gel would be appreciated. Second, does anyone have a suggestion on how to repair the cells that are embedded in the tissue paper thick "cell block"? Thanks guys and gals! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ********* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ************************************************************************ ********* From sgoebel <@t> mirnarx.com Wed Mar 9 08:56:05 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Mar 9 08:56:11 2011 Subject: [Histonet] diastase In-Reply-To: References: Message-ID: Just hack up some spit... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, March 09, 2011 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] diastase I am looking to purchase diastase to use for the PAS/DIASTASE stain. I see there are many options in the catalogues. What is recommended? diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micropathlabs <@t> yahoo.com Wed Mar 9 08:57:33 2011 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Wed Mar 9 08:57:36 2011 Subject: [Histonet] Extended processing for breasts Message-ID: <91462.43890.qm@web161711.mail.bf1.yahoo.com> Would someone who has extended their processing times to accomodate the CAP regs for breast tissue be willing to share their processing schedules with me? Up until now, we've had someone?in over the weekend to add breast tissue to processor but are having staffing?issues so I'm looking for alternatives. Thanks a bunch! ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From wbenton <@t> cua.md Wed Mar 9 11:01:29 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Mar 9 11:07:46 2011 Subject: [Histonet] Extended processing for breasts In-Reply-To: <91462.43890.qm@web161711.mail.bf1.yahoo.com> References: <91462.43890.qm@web161711.mail.bf1.yahoo.com> Message-ID: <0B8979A204680A42B93A52B486088CD9171120541B@CUAEXH1.GCU-MD.local> Generally the easiest thing to do is to extend your time in the 70% ETOH or other low percentage ETOH. This allows you to have a cut off on the weekend vs Friday. We had times of 24 to 48 hours depending upon weekend holiday rotations etc... Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas [micropathlabs@yahoo.com] Sent: Wednesday, March 09, 2011 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Extended processing for breasts Would someone who has extended their processing times to accomodate the CAP regs for breast tissue be willing to share their processing schedules with me? Up until now, we've had someone in over the weekend to add breast tissue to processor but are having staffing issues so I'm looking for alternatives. Thanks a bunch! Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From SSeguin <@t> hrsrh.on.ca Wed Mar 9 12:04:35 2011 From: SSeguin <@t> hrsrh.on.ca (Seguin, Suzanne) Date: Wed Mar 9 12:04:45 2011 Subject: [Histonet] RE:FORMALIN CONTAINERS Message-ID: Hello, Does anyone clean and re-use large specimen containers? If so, how do you prepare the containers for cleaning to ensure the "cleaning staff" are not exposed to residual formalin? Thanks Sue ************************************************************************ The information contained in this e-mail and document(s) attached are for the exclusive use of the addressee and may contain confidential, privileged and non-disclosable information. If the recipient of this e-mail is not the addressee, such recipient is strictly prohibited from reading, photocopying, distributing or otherwise using this e-mail or its content in any way. From etambutte <@t> centrescientifique.mc Wed Mar 9 12:07:52 2011 From: etambutte <@t> centrescientifique.mc (Eric Tambutte) Date: Wed Mar 9 12:08:00 2011 Subject: [Histonet] Re: Histonet Digest, Vol 88, Issue 11 Message-ID: <1047107834@s15272523.onlinehome-server.info> Merci pour votre message. Je suis actuellement absent du laboratoire. Je repondrai a votre message a mon retour a partir du 15 mars 2011. En cas d'urgence merci de contacter : Sylvie Tambutté (stambutte@centrescientifique.mc) - +377 93 30 12 11. Thank you for your email. I am currently out of the lab. I will reply to your message after the 15th of march 2011. For any problem, contact Dr Sylvie Tambutté (stambutte@centrescientifique.mc). From Judith_Pardue <@t> memorial.org Wed Mar 9 12:14:46 2011 From: Judith_Pardue <@t> memorial.org (Pardue, Judith) Date: Wed Mar 9 12:15:02 2011 Subject: [Histonet] Pulling apart sections Message-ID: We use a teasing needle to pull apart our section. Jusith Pardue Memorial Health Care System Chattanooga, Tn. 37404 This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Wanda.Smith <@t> HCAhealthcare.com Wed Mar 9 12:15:50 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Wed Mar 9 12:15:56 2011 Subject: [Histonet] RE: RE:FORMALIN CONTAINERS In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A0B262D4@NADCWPMSGCMS03.hca.corpad.net> We do not re-use large specimen containers. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Seguin, Suzanne Sent: Wednesday, March 09, 2011 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE:FORMALIN CONTAINERS Hello, Does anyone clean and re-use large specimen containers? If so, how do you prepare the containers for cleaning to ensure the "cleaning staff" are not exposed to residual formalin? Thanks Sue ************************************************************************ The information contained in this e-mail and document(s) attached are for the exclusive use of the addressee and may contain confidential, privileged and non-disclosable information. If the recipient of this e-mail is not the addressee, such recipient is strictly prohibited from reading, photocopying, distributing or otherwise using this e-mail or its content in any way. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Wed Mar 9 12:17:55 2011 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Mar 9 12:18:30 2011 Subject: [Histonet] band saws In-Reply-To: <25A4DE08332B19499904459F00AAACB7194516A72C@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB7194516A72C@EVS1.archildrens.org> Message-ID: <4EBFF65383B74D49995298C4976D1D5E07DA1D3D@LSRIEXCH1.lsmaster.lifespan.org> You don't need a large powerful band saw to cut bone. Bone is quite soft compared to the kinds of substances, particularly metals, which those larger band saws can handle. I bought one for my lab over 12 years ago, the smallest, cheapest Craftsman model Sears carried, and it's still going strong. I use it for bone work and also for trimming methyl methacrylate blocks. The most important thing is the type of blade you use. Blades designed for general cutting of wood are too coarse (the teeth are too large) for bone work. A plywood cutting blade might be ok, but I find that blades designed for cutting metal are best. The teeth are much finer (I use blades with 14 teeth per inch), and they have the added advantage of staying sharp a lot longer than a wood cutting blade. From jqb7 <@t> cdc.gov Wed Mar 9 12:20:08 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Mar 9 12:20:50 2011 Subject: [Histonet] RE: Pulling apart sections In-Reply-To: References: Message-ID: I use very fine-pointed curved forceps Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Wednesday, March 09, 2011 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pulling apart sections We use a teasing needle to pull apart our section. Jusith Pardue Memorial Health Care System Chattanooga, Tn. 37404 This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mary.L.Ring <@t> HealthPartners.Com Wed Mar 9 12:28:43 2011 From: Mary.L.Ring <@t> HealthPartners.Com (Ring, Mary L) Date: Wed Mar 9 12:28:47 2011 Subject: [Histonet] CMV Message-ID: <2594D39B69223047B6E2F4ADF1E62DB9010FF9628177@HPEMX3.HealthPartners.int> I am looking for a CMV antibody that is IVD. I am hoping to find a good vendor that distributes it. Thanks! Mary Ring, HT, QIHC Regions Hospital 640 Jackson St St Paul, Mn 55101 mary.l.ring@healthpartners.com ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From jqb7 <@t> cdc.gov Wed Mar 9 12:37:04 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Mar 9 12:37:16 2011 Subject: [Histonet] DAKO Artisan Kit Issues In-Reply-To: <326758572.1743298.1299527535381.JavaMail.root@cl04-host04.dlls.pa.frontiernet.net> References: <772445744.1743205.1299527226099.JavaMail.root@cl04-host04.dlls.pa.frontiernet.net> <326758572.1743298.1299527535381.JavaMail.root@cl04-host04.dlls.pa.frontiernet.net> Message-ID: We periodically have plunger and leaking issues.....they always replace the kits. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mizlyght@frontier.com Sent: Monday, March 07, 2011 2:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAKO Artisan Kit Issues Fellow Histologists: This is my first time using Histonet but I need to ask if anyone has started having problems with their DAKO Artisan kits recently? We have started having leaking kits for Gram Lot # 10043062 and Expiration Date 4/30/2011, and plunger issues with the Iron Lot # 10047616 Expiration Date 10/31/2011. We have also found that the GMS kits Lot # 10049354 and Expiration 11/30/2011 we have received in the last two shipments are from the same lot but they do not work with our validated protocol. What is strange is that the previous GMS kit worked on the same control that we are using now, but does not work with the new kit, but when stained with PAS-Fungus we see the same fungus as we saw with the previous GMS kit. I think it could be something with the shipping method but wanted to see if anyone else has seen a difference. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jryan <@t> sleh.com Wed Mar 9 16:22:24 2011 From: jryan <@t> sleh.com (Ryan, John P.) Date: Wed Mar 9 16:22:33 2011 Subject: [Histonet] barcode scanner for tissue cassette Message-ID: Can anyone recommend a suitable barcode scanner that will work with the ThermoFisher Microwriter? I have 5 units that are currently using either a Symbol or a Cognex scanner that do not function appropriately. Requires multiple passes (4-6 if at all) of the 2D labeled cassette in order for the slide to print. Very seldom does it print on the 1st pass. When you are dealing with 500 - 700 cassettes there is no time for multiple passes. I don't know if it is the scanners, software or just settings. I have not had much success from the company regarding updating the scanners to help resolve this issue. John Ryan Assistant Administrative Director Pathology 832-355-2643 phone 832-355-4232 fax jryan@sleh.com +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. From mike <@t> pathview.com Wed Mar 9 16:50:18 2011 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Mar 9 16:51:00 2011 Subject: [Histonet] barcode scanner for tissue cassette In-Reply-To: References: Message-ID: <020701cbdeac$6555e3e0$3001aba0$@pathview.com> John, have your tried pastel colored cassettes? Scanning works much better with those. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ryan, John P. Sent: Wednesday, March 09, 2011 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] barcode scanner for tissue cassette Can anyone recommend a suitable barcode scanner that will work with the ThermoFisher Microwriter? I have 5 units that are currently using either a Symbol or a Cognex scanner that do not function appropriately. Requires multiple passes (4-6 if at all) of the 2D labeled cassette in order for the slide to print. Very seldom does it print on the 1st pass. When you are dealing with 500 - 700 cassettes there is no time for multiple passes. I don't know if it is the scanners, software or just settings. I have not had much success from the company regarding updating the scanners to help resolve this issue. John Ryan Assistant Administrative Director Pathology 832-355-2643 phone 832-355-4232 fax jryan@sleh.com +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aamador <@t> ameripath.com Wed Mar 9 21:22:01 2011 From: aamador <@t> ameripath.com (Amador, Amanda) Date: Wed Mar 9 21:22:10 2011 Subject: [Histonet] Control Slides Message-ID: <1D9B439FC446244095353DCA4FC23DD204F445CBBA@MWNMAIL00.ameripath.local> Is there guidelines for how long special stains controls are good for once they are cut? We have spirochetes for our Steiner that is from 2007 and we are having issues. Amanda Amador, HT(ASCP)CM AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | Indianapolis, IN 46219 | phone 317.275.8052 | aamador@ameripath.com | www.AmeriPath.com From irena.kirbis <@t> hotmail.com Thu Mar 10 01:18:04 2011 From: irena.kirbis <@t> hotmail.com (IRENA SREBOTNIK KIRBIS) Date: Thu Mar 10 01:18:08 2011 Subject: [Histonet] ArrayMold System In-Reply-To: <064301362827D243B0F0D3B63E70906305644C44@PHSXMB24.partners.org> References: <064301362827D243B0F0D3B63E70906305644C44@PHSXMB24.partners.org> Message-ID: Hi, we have Arraymold more than a year now and we prepared around 10 TM's with it, it is really simple, easy and effective, however we found instructions to keep contructed TM overnight on 37 degrees uneffective since recipient and donor paraffin were not blend enough, we keep our TM's overnight on 40 degrees and next day half an hour on 60 (in moulds) if necessary we even prolonge this phase, this also help us to achieve that shorter tissue bars come down to the level. regards Irena Kirbis > Date: Tue, 8 Mar 2011 10:59:46 -0500 > From: AlieteE_Langsdorf@DFCI.HARVARD.EDU > To: histonet@lists.utsouthwestern.edu > Subject: re: [Histonet] ArrayMold System > > Hi, > I am also interested in hearing about the ArrayMold vs Beecher vs any other > do-it-yourself TMA systems. Any favorites systems/recommendations or > suggestions? > Thanks > ~Ally > Senior Research Technician > Medical Oncology > Dana-Farber Cancer Institute > re: > Wondering if anyone has used/purchased the ArrayMold "tissue micro array > system"?? Positive and negative feedback would be greatly appreciated :) > > Nancy Heath, HT(ASCP) > > March 10, 2011 is Histotechnology Professionals Day > > > Senior Research Technician > Medical Oncology > Dana-Farber Cancer Institute > > > > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfonner <@t> labpath.com Thu Mar 10 06:52:48 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Thu Mar 10 06:55:56 2011 Subject: [Histonet] Control Slides In-Reply-To: <1D9B439FC446244095353DCA4FC23DD204F445CBBA@MWNMAIL00.ameripath.local> References: <1D9B439FC446244095353DCA4FC23DD204F445CBBA@MWNMAIL00.ameripath.local> Message-ID: <002801cbdf22$0f458ed0$2dd0ac70$@com> Amanda, You will lose antigenicity on the spirochete control slides after that long of a period. We usually cut no more than 10 in advance, and we keep them in the refrigerator. Hope this helps. Sheila HT (ASCP) Knoxville Dermatopathology Lab Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amador, Amanda Sent: Wednesday, March 09, 2011 10:22 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Control Slides Is there guidelines for how long special stains controls are good for once they are cut? We have spirochetes for our Steiner that is from 2007 and we are having issues. Amanda Amador, HT(ASCP)CM AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | Indianapolis, IN 46219 | phone 317.275.8052 | aamador@ameripath.com | www.AmeriPath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Derek.Papalegis <@t> tufts.edu Thu Mar 10 07:49:20 2011 From: Derek.Papalegis <@t> tufts.edu (Papalegis, Derek) Date: Thu Mar 10 07:49:25 2011 Subject: [Histonet] Histologist Position at Tufts University Boston Message-ID: <9A844F6D6E1A964AA87DEBB12ADD4A2B0857F730@TFTMEXDAG01MB01.tufts.ad.tufts.edu> Histotechnologist Position at Tufts University, Boston Tufts University Division of Laboratory Animal Medicine (DLAM) in Boston, MA, has an immediate opening for a histotechnologist to provide research and diagnostic histology and immunohistochemistry support for Tufts University and Tufts Medical Center researchers. The histotechnologist will manage day-to-day operations of the Animal Histology Core under the guidance of the veterinary pathologist in the Research Animal Health and Pathology Support Laboratory and will work closely with investigators to meet their research histology needs. The histotechnologist is expected to function independently to manage technical operations of the laboratory with minimal supervision from the pathologist. Responsibilities include specimen processing; cryostat sectioning; slide production; routine, histochemical and immunohistochemical (IHC) staining; IHC protocol development; meeting quality control standards; assisting the pathologist as needed; training investigative staff; and development of new services for the laboratory. Applicants must be eligible or certified as American Society of Clinical Pathology (ASCP) Histotechnician (HT) or Histotechnologist (HTL) and have an Associate's Degree or higher. At least 3-5 years experience, including experience in immunohistochemistry and experience with animal tissues, is preferred. For further information or to apply: https://recruiter.kenexa.com/tufts/cc/CCJobDetailAction.ss?command=CCViewDetail&job_REQUISITION_NUMBER=56133&ccid=bupJEdUjsTs%3D Please use the link above if you require more information or would like to apply. From Janet.Bonner <@t> FLHOSP.ORG Thu Mar 10 08:53:54 2011 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu Mar 10 08:54:52 2011 Subject: [Histonet] diastase In-Reply-To: References: , Message-ID: <85F2E7DD5D91744D831A66E44D718B9A21C2DEB1@fhovxchmb7001.ADVENTISTCORP.NET> Porcine amylase has worked for us (of course, so has the spit!) Janet ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com [sgoebel@mirnarx.com] Sent: Wednesday, March 09, 2011 9:56 AM To: dmccaig@ckha.on.ca; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] diastase Just hack up some spit... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, March 09, 2011 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] diastase I am looking to purchase diastase to use for the PAS/DIASTASE stain. I see there are many options in the catalogues. What is recommended? diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Thu Mar 10 09:48:19 2011 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Thu Mar 10 09:48:23 2011 Subject: [Histonet] Happy Histo Day Message-ID: <9981.39532.qm@web43515.mail.sp1.yahoo.com> Happy Histotechnology Day Everyone....I want to pass this on from Sharon Wehman?of Sakura Finetek. Thank you Sharon! It gives me great pleasure to celebrate this day and give thanks to the wonderful histotechs! ? ? -Thank you for dedicating your expertise and knowledge to every specimen, block, slide and stain ? -Thank you for being a part of and making a difference in every diagnosis ? -Thank you for working hard every day and going above and beyond on most days ? -Thank you for the incredible care and consideration you give to every patient, even though most do not realize what you do--that is EXCELLENCE! ? ? Excellence is never an accident; it is the result of high intention, sincere effort, intelligent direction, skillful execution and the vision to see obstacles as opportunities. ? ? May Your Histotechnology Day Be As Wonderful As You Are! ? Sharon Wehman Area Manager Sakura Finetek USA, Inc. 1750 West 214th Street Torrance, CA 90501 Office:800-725-8723 X:7274 Cell:678-462-6349 swehman@sakuraus.com www.sakuraus.com ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From relia1 <@t> earthlink.net Thu Mar 10 09:52:59 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Mar 10 09:53:04 2011 Subject: [Histonet] Happy National Histotechnology Day from Pam Barker at RELIA Solutions Message-ID: <7915D913DC144740AB8DBD6D92CFD9DA@ownerf1abaad51> Hi Histonetters! How are you? I wanted to take a moment and say Happy Histotechnology Professionals Day. I want to thank you not only for the job that you do and the lives that you save but also for allowing me to work with you in improving your careers. In all my years of recruiting I have never worked with a more professional, appreciative, honest and truly enjoyable group of people. Kudos to you!! I am curious as to how everyone has celebrated today. I starting celebrating yesterday by contacting each and every one of my 1000+ client/employers to let them know that today is National Histotechnology Professionals Day. Hopefully, if they weren't already doing something to show their appreciation they are now!! I hope everyone has a great day!! I do have some BRAND NEW positions that I would like to tell you about as well. Here is a list of my current openings. All of these positions are fulltime permanent positions and my clients offer excellent salaries, benefits and relocation assistance. HISTOLOGY/PATHOLOGY MANAGEMENT ME ? Histology Supervisor ? Scarborough, ME CA - Histology Lab Manager ? Central Valley of CA CO ? Histology Manager ? Colorado Springs LA - Histology Supervisor ? Baton Rouge, LA WI ? IHC Specialist/Supervisor -Milwaukee HISTOTECHS FL ? Fort Myers ? Dermpath Histotech FL ? Panama City Fl. Lic histotechnologist MA ? North of Boston dermpath exper a plus. NC ? Charlotte Mohs Tech NC ? Charlotte Dermpath Histotech PA ? Southern PA Dermpath Histotech ME ? Scarborough Histology Tech. IL ? Chicago Histology Training Specialist WI ? Lead Histotech/Immunohistochemistry Specialist OR ? Portland Histotech PATHOLOGY/PATHOLOGIST'S ASSISTANTS NC ? Charlotte PA grad from NAACLES program required NY ? Lead Medical Technologist ? Generalist/Days MA ? Medical Technologist - Generalist If you or anyone you know might be interested in any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net. Happy Histo Day!! Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia www.twitter.com/pamatrelia From nancy_schmitt <@t> pa-ucl.com Thu Mar 10 10:07:02 2011 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Mar 10 10:07:08 2011 Subject: [Histonet] BOND Max vs. BOND III Message-ID: <737BD0BF52F0744B96B74B61756AC06444D26F6C43@hestia.ad.pa-ucl.com> Happy Histotechnology Professional Day! We currently have a Bond Max and are happy with it - looking for users of the Bond III to see if you like it - is it really almost maintenance free? - is it really faster? Honest opinions appreciated! Thanks Nancy Schmitt HT, MLT(ASCP) Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From jshelley <@t> sanfordburnham.org Thu Mar 10 10:25:50 2011 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Thu Mar 10 10:26:01 2011 Subject: [Histonet] TTC staining & Happy Histotechnology Professionals Day Message-ID: I hope all of you are being appreciated today and really every day for the hard work that you do each and every day to make a difference in people's lives. Now down to some business, I was hoping to talk with someone either on line or off line about TTC staining on infracted hearts and how to perform this technique. Whether it is better to perfuse or to slice the hearts and then stain. I really need someone to mentor me through the whole process. Thanks in advance for whatever help you may be able to give me. Kind Regards! John J Shelley From cverdick <@t> slu.edu Thu Mar 10 10:27:07 2011 From: cverdick <@t> slu.edu (Crystal Verdick) Date: Thu Mar 10 10:27:27 2011 Subject: [Histonet] celloidin tissue staining Message-ID: I am currently working with 30-40 year old celloidin embedded tissue that I have sectioned myself. I have used a myelin stain of a modified Weigert's and am trying to get a Nissl stain to work with no luck. The 40 micron sections are being kept in 70% etoh in individual series jars. Weigert's stain works perfectly however when I attempt to stain for Nissl substance the sections turn out blotchy, uneven and faded as if the background is the only thing that is holding the stain. I have tried soaking the sections in 50% etoh for at least an hour before going into dH20 for 2 hours up to 2 days. Nothing has worked. Does anyone have any ideas that might help? Something to dissolve a possible mordant? My only other option is to dissolve the celloidin in a 50/50 mixture of absolute etoh and ether which could damage the tissue/structures within. Crystal V. Saint Louis University Center for Anatomical Science Education From histotalk <@t> yahoo.com Thu Mar 10 10:53:57 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Thu Mar 10 10:54:01 2011 Subject: [Histonet] It's March 10th. . . Message-ID: <878834.2274.qm@web120619.mail.ne1.yahoo.com> Hi HistoNetters - Have an absolutely, marvelous, fantastic and needless to say,?HAPPY Histotechnology Professionals Day! I've been plugging it on my show since January 30th,?so I hope everyone is celebrating "OUR" day! Regards, David www.HistoTALK.com From esulkosky <@t> gmail.com Thu Mar 10 15:14:52 2011 From: esulkosky <@t> gmail.com (Eric Sulkosky) Date: Thu Mar 10 15:14:55 2011 Subject: [Histonet] Job Post for NY Message-ID: New Urology Histology lab opening in Booklyn, NY. Hiring full and partime histology techs. If interested please send cv and letters of interests to Ellen Barbarino ebarbarino@siradonc.com Thanks and have a great day! From thomas.crowell <@t> novartis.com Thu Mar 10 15:20:00 2011 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Thu Mar 10 15:20:09 2011 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 03/10/2011 and will not return until 03/14/2011. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. From Bonnie.Whitaker <@t> osumc.edu Thu Mar 10 15:35:46 2011 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Thu Mar 10 15:36:00 2011 Subject: [Histonet] Ohio State Clinical Histology Manager Position Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670618732A38@EXMBOX-VP05.OSUMC.EDU> Hi Everyone, We are still searching for the right person to fill the Clinical Histology Manager position at The Ohio State University Medical Center in Columbus, Ohio. The lab does a brisk volume, our daily block count is around 760. We are open from Sunday evening until Saturday morning, with call rotation for Saturday morning through Sunday evening. It is a great group of people to work with. The official blurb is: The OSUMC clinical histology laboratory is a functional laboratory within the Anatomic Pathology branch serving the pathology subspecialty divisions; Histology Lab Manager will provide leadership for daily operations of the histology labs; manages human resources, financial and budget planning and preparation, new test development, quality improvement, safety programs, compliance program, successful inspection and accreditation processes, and ensuring current policies and procedures related to technical, operational, and administrative areas of the laboratory are adhered to. We prefer someone with a solid background in clinical histology, as well as supervisory experience. If you are interested, please send me an email with your current resume/CV. Thanks! Bonnie Bonnie Whitaker AP Operations Director The Ohio State University Medical Center Anatomic Pathology Branch Department of Pathology N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 Bonnie.Whitaker@osumc.edu phone 614.293.5048 fax 614.293.7273 pager 614.346.5013 From CKent <@t> mbhs.org Thu Mar 10 16:00:46 2011 From: CKent <@t> mbhs.org (CKent@mbhs.org) Date: Thu Mar 10 16:00:51 2011 Subject: [Histonet] AUTO: Carolyn J Kent is out of the office. (returning 03/11/2011) Message-ID: I am out of the office until 03/11/2011. I will respond to your message when I return. Note: This is an automated response to your message "Histonet Digest, Vol 88, Issue 12" sent on 3/10/2011 12:05:55 PM. This is the only notification you will receive while this person is away. From AnthonyH <@t> chw.edu.au Thu Mar 10 16:11:29 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Mar 10 16:11:53 2011 Subject: [Histonet] Control Slides In-Reply-To: <002801cbdf22$0f458ed0$2dd0ac70$@com> References: <1D9B439FC446244095353DCA4FC23DD204F445CBBA@MWNMAIL00.ameripath.local> <002801cbdf22$0f458ed0$2dd0ac70$@com> Message-ID: <6D6BD1DE8A5571489398B392A38A715718832DC9@xmdb02.nch.kids> Sheila, I believe that Amanda is referring to control slides cut for the Steiner Modification of the Warthin-Starry stain not for immunohistochemistry. I would be surprised if the control slides for the Steiner's would "go off". It is possible that the technique has gone out of control. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Thursday, 10 March 2011 11:53 PM To: 'Amador, Amanda'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Control Slides Amanda, You will lose antigenicity on the spirochete control slides after that long of a period. We usually cut no more than 10 in advance, and we keep them in the refrigerator. Hope this helps. Sheila HT (ASCP) Knoxville Dermatopathology Lab Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amador, Amanda Sent: Wednesday, March 09, 2011 10:22 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Control Slides Is there guidelines for how long special stains controls are good for once they are cut? We have spirochetes for our Steiner that is from 2007 and we are having issues. Amanda Amador, HT(ASCP)CM AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | Indianapolis, IN 46219 | phone 317.275.8052 | aamador@ameripath.com | www.AmeriPath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From DSiena <@t> statlab.com Thu Mar 10 16:17:42 2011 From: DSiena <@t> statlab.com (Debra Siena) Date: Thu Mar 10 16:17:49 2011 Subject: [Histonet] Control Slides In-Reply-To: <6D6BD1DE8A5571489398B392A38A715718832DC9@xmdb02.nch.kids> References: <1D9B439FC446244095353DCA4FC23DD204F445CBBA@MWNMAIL00.ameripath.local> <002801cbdf22$0f458ed0$2dd0ac70$@com> <6D6BD1DE8A5571489398B392A38A715718832DC9@xmdb02.nch.kids> Message-ID: One of the things that I usually suggest is that when every time that you cut the control, sequentially number the slides and test the first section and the last to make sure that you have not cut through the area of interest and keep that slide as part of the quality assurance/quality control. If both sections fit, you could even put on the same slide and mark which is which for cost and space savings. Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products Direct: 972-436-1010? x229 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Thursday, March 10, 2011 4:11 PM To: 'Sheila Fonner'; 'Amador, Amanda'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Control Slides Sheila, I believe that Amanda is referring to control slides cut for the Steiner Modification of the Warthin-Starry stain not for immunohistochemistry. I would be surprised if the control slides for the Steiner's would "go off". It is possible that the technique has gone out of control. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Thursday, 10 March 2011 11:53 PM To: 'Amador, Amanda'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Control Slides Amanda, You will lose antigenicity on the spirochete control slides after that long of a period. We usually cut no more than 10 in advance, and we keep them in the refrigerator. Hope this helps. Sheila HT (ASCP) Knoxville Dermatopathology Lab Knoxville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amador, Amanda Sent: Wednesday, March 09, 2011 10:22 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Control Slides Is there guidelines for how long special stains controls are good for once they are cut? We have spirochetes for our Steiner that is from 2007 and we are having issues. Amanda Amador, HT(ASCP)CM AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | Indianapolis, IN 46219 | phone 317.275.8052 | aamador@ameripath.com | www.AmeriPath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Thu Mar 10 18:38:56 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Mar 10 18:38:59 2011 Subject: [Histonet] Control Slides In-Reply-To: <1D9B439FC446244095353DCA4FC23DD204F445CBBA@MWNMAIL00.ameripath.local> References: <1D9B439FC446244095353DCA4FC23DD204F445CBBA@MWNMAIL00.ameripath.local> Message-ID: <48441ED607EC40B6AF54090AB8300EA1@HP2010> I've noticed that our spirochete control slides don't stain as intense starting about 3 months after they are sectioned, and that they stop staining by about 6 months. (This is with a silver stain.) I've talked with other histotechs, and they say they've seen the same phenomenon with AFB and Gram controls. (We use ours up too quickly - we never have 6-12 months old control slides for these, so I can't attest to this. ) Bancroft's book talks about this phenomenon with amyloid, and suggests that oxidation of the tissue (proteins) due to exposure to air may be the cause. I'm guessing the it probably applies to the precut microorganism control slides, too. We only cut enough slides for 6 months, and date them. Other people say they put their cut control slides in a slide box with a lid after drying, and then place the box in the refrig, as cold slows down the chemical change, and the lid keeps the moving air off the slide. Other people say that, after they dry the slide, they dip the slide in paraffin, to cover the tissue, so that the air doesn't touch the tissue during the months before the slide is used in a stain. Just 3 suggestions to stop this problem. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Amador, Amanda" Sent: Wednesday, March 09, 2011 10:22 PM To: Subject: [Histonet] Control Slides > Is there guidelines for how long special stains controls are good for once > they are cut? We have spirochetes for our Steiner that is from 2007 and > we are having issues. > > Amanda Amador, HT(ASCP)CM > AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | > Indianapolis, IN 46219 | phone 317.275.8052 | > aamador@ameripath.com | > www.AmeriPath.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From David.Oelrich <@t> HealthTronics.com Thu Mar 10 18:38:17 2011 From: David.Oelrich <@t> HealthTronics.com (Oelrich, David) Date: Thu Mar 10 18:40:08 2011 Subject: [Histonet] Histology Manager Position in Augusta, GA. Message-ID: <0A7857FCB16E6045B1035A04BB5C2B321897BC33F3@PA100-EX-MBXC01.Endo.com> Hello everyone, Just a note to let you all know we have an opening in our Augusta, GA lab for a Histology Manager. The position is a combination bench/manager position supervising 5-6 techs in a reference/specialty Urology AP lab. Great facility, great team, great city. For more info or to apply: http://tbe.taleo.net/NA6/ats/careers/requisition.jsp?org=ENDO&cws=8&rid=2594 and www.healthtronics.com/lab Relocation funds available for good candidates. Excellent benefits and competitive pay. Happy cutting! Dave Oelrich HealthTronics From pruegg <@t> ihctech.net Thu Mar 10 20:28:46 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Mar 10 20:28:50 2011 Subject: [Histonet] expired abs Message-ID: Just wanted to remind everyone if you are about to discard expired abs I would be willing to take them and put them to good use. If you have expired abs to get rid of contact me and I will provide you a fedex acct. # so you can send them to me rather than discard. This effort paid off recently because I sent a care package of expired abs and detection reagents to a hospital tech in Ghana West Africa who is using them to test patients there because they have training in ihc but do not have the abs or detection reagents to do it. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org From member <@t> linkedin.com Thu Mar 10 22:12:14 2011 From: member <@t> linkedin.com (Joyce Bidwell via LinkedIn) Date: Thu Mar 10 22:12:17 2011 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <1785619943.4693951.1299816734361.JavaMail.app@ela4-bed35.prod> LinkedIn ------------Joyce Bidwell requested to add you as a connection on LinkedIn: ------------------------------------------ Jackie, I'd like to add you to my professional network on LinkedIn. - Joyce Accept invitation from Joyce Bidwell http://www.linkedin.com/e/yvpgd1-gl4lbn2r-65/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1187336310_3/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPnP0NcPoPcPsUcj59bRhCul5ehnkRbPkMdzgQcPgUd38LrCBxbOYWrSlI/EML_comm_afe/ View invitation from Joyce Bidwell http://www.linkedin.com/e/yvpgd1-gl4lbn2r-65/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1187336310_3/3dvc34PdzcPdPwNckALqnpPbOYWrSlI/svi/ -- (c) 2011, LinkedIn Corporation From CIngles <@t> uwhealth.org Thu Mar 10 22:17:29 2011 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Mar 10 22:18:28 2011 Subject: [Histonet] expired abs References: Message-ID: I think my "abs" expired long ago! Happy Friday Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patsy Ruegg Sent: Thu 3/10/2011 8:28 PM To: 'ihcrg Group (E-mail)'; histonet@lists.utsouthwestern.edu Subject: [Histonet] expired abs Just wanted to remind everyone if you are about to discard expired abs I would be willing to take them and put them to good use. If you have expired abs to get rid of contact me and I will provide you a fedex acct. # so you can send them to me rather than discard. This effort paid off recently because I sent a care package of expired abs and detection reagents to a hospital tech in Ghana West Africa who is using them to test patients there because they have training in ihc but do not have the abs or detection reagents to do it. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Fri Mar 11 04:21:19 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Mar 11 04:21:21 2011 Subject: [Histonet] old dyes Message-ID: I was just given some old bottles of dyes (powder form) (20+ years old), from a lab that was cleaning out there cupboards. I kept about 2/3 of them. The rest I either already had, could not figure out a use for them, or they are no longer being made (so if I found a use for it, once that bottle was gone, the procedure could never be done again). Is there anyone out there that can use some old dyes, maybe some vendor that holds on to old dyes, to refer back to them to try to create new dyes? Please email me at work (pwenk@beaumont.edu ), not via Histonet, which goes to my home. I can send you the list of dye powders. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 From joachim.hehl <@t> lmc.biol.ethz.ch Fri Mar 11 04:48:26 2011 From: joachim.hehl <@t> lmc.biol.ethz.ch (Hehl Joachim) Date: Fri Mar 11 04:48:33 2011 Subject: [Histonet] Paraffin vs. Cryostat Message-ID: Dear all, I have a very basic (maybe stupid) question regarding the problem when should one use paraffin sections or better cryo sections. Since I have heard so different opinions I would be glad to receive some founded recommendations and/or literature references comparing these two techniques. Thanks for your help! Joachim From amitapandey <@t> torrentpharma.com Fri Mar 11 04:53:53 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Fri Mar 11 04:57:19 2011 Subject: [Histonet] Polymer detection kit In-Reply-To: References: Message-ID: Dear Histonet fellow, I am planning to use Bio care detection kit -MRT511 (for mouse monoclonal antibody on rat kidney FFPE) and RMR622 (for rabbit polyclonal antibody on rat kidneyFFPE) Do i have to buy any additional reagents to perform the staining.(specially blocker or antigen retrieval) Any feed back on their results and trouble shootings are welcome. Amita From lpwenk <@t> sbcglobal.net Fri Mar 11 05:02:31 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Mar 11 05:02:34 2011 Subject: [Histonet] Paraffin vs. Cryostat In-Reply-To: References: Message-ID: <6D1DF71B5E0B4B48A5A2B064AD91C82D@HP2010> Frozen sections (cryostat) are for when: - there is a need for speed - when fixing and processing tissue through to paraffin would destroy/extract the component needed for diagnosis Speed - such as when the patient is still on the operating table and a diagnosis is needed immediately Fixation/Processing destroy/extract - such as demonstration of lipids, muscle enzymes, some antigens Paraffin embedded tissue also allows for thinner sections (e.g. 2 um kidneys), cutting of harder tissues (e.g., decalcified bone), and easier to store and retrieve archival blocks (store paraffin blocks at room temp for decades vs. needing a huge freezer for frozen tissue and hope for no freezer burn (freeze-dried) tissue in a couple of years). Well fixed processed tissue is no longer infectious (ignoring prions), while frozen tissue is still biohazardous. So most people work with fixed processed tissues most of the time, and use frozen sections only when needed (time, extraction reasons above). Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Hehl Joachim" Sent: Friday, March 11, 2011 5:48 AM To: Subject: [Histonet] Paraffin vs. Cryostat > Dear all, > I have a very basic (maybe stupid) question regarding the problem when > should one use paraffin sections or better cryo sections. Since I have > heard > so different opinions I would be glad to receive some founded > recommendations and/or literature references comparing these two > techniques. > Thanks for your help! > Joachim > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amitapandey <@t> torrentpharma.com Fri Mar 11 06:11:42 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Fri Mar 11 06:15:07 2011 Subject: [Histonet] Paraffin vs. Cryostat In-Reply-To: References: Message-ID: Frozen sections are not good to study the histology. Frozen are better for immediate diagnosis , enzyme study and special staining like IHC- where formalin masked some of antigens. Amita From: "Hehl Joachim" To: "histonet@lists.utsouthwestern.edu" Date: 11/03/11 04:20 PM Subject: [Histonet] Paraffin vs. Cryostat Sent by: histonet-bounces@lists.utsouthwestern.edu Dear all, I have a very basic (maybe stupid) question regarding the problem when should one use paraffin sections or better cryo sections. Since I have heard so different opinions I would be glad to receive some founded recommendations and/or literature references comparing these two techniques. Thanks for your help! Joachim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TroyerDA <@t> EVMS.EDU Fri Mar 11 06:40:14 2011 From: TroyerDA <@t> EVMS.EDU (Troyer, Dean A.) Date: Fri Mar 11 06:40:18 2011 Subject: [Histonet] processing animal and human clinical diagnostic tissue Message-ID: <71EE6DB05CB563458E8CBA495B3E4BD003F5629F@romulus.evms.net> What are the regulations and/or best practice guidelines concerning processing and sectioning animal tissues and human clinical diagnostic tissues on the same tissue processors, microtomes, etc.? Comments welcome also on fresh frozen (cryosections) vs paraffin. Kind Regards, Dean Troyer troyerda@evms.edu From b-frederick <@t> northwestern.edu Fri Mar 11 08:40:55 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Mar 11 08:42:38 2011 Subject: [Histonet] expired abs In-Reply-To: Message-ID: <97A58F9C7F374D2F9877A16516CDB88C@lurie.northwestern.edu> I don't think mine were ever made,let alone expired! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Thursday, March 10, 2011 10:17 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] expired abs I think my "abs" expired long ago! Happy Friday Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patsy Ruegg Sent: Thu 3/10/2011 8:28 PM To: 'ihcrg Group (E-mail)'; histonet@lists.utsouthwestern.edu Subject: [Histonet] expired abs Just wanted to remind everyone if you are about to discard expired abs I would be willing to take them and put them to good use. If you have expired abs to get rid of contact me and I will provide you a fedex acct. # so you can send them to me rather than discard. This effort paid off recently because I sent a care package of expired abs and detection reagents to a hospital tech in Ghana West Africa who is using them to test patients there because they have training in ihc but do not have the abs or detection reagents to do it. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Fri Mar 11 08:54:52 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Fri Mar 11 08:54:57 2011 Subject: [Histonet] Polymer detection kit In-Reply-To: References: Message-ID: I use the Biocare polymers all the time because they are cheaper than DAKO. Some of them are biotin free, so as long as your primary has no biotin and you aren't staining anything with endogenous biotin you can omit that block. Other than that enzyme and protein blocking I still do. Some of them are a two step process and some are one solution. They work great! Just follow the protocol included with the polymer =) Good Luck!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of amitapandey@torrentpharma.com Sent: Friday, March 11, 2011 4:54 AM To: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Polymer detection kit Dear Histonet fellow, I am planning to use Bio care detection kit -MRT511 (for mouse monoclonal antibody on rat kidney FFPE) and RMR622 (for rabbit polyclonal antibody on rat kidneyFFPE) Do i have to buy any additional reagents to perform the staining.(specially blocker or antigen retrieval) Any feed back on their results and trouble shootings are welcome. Amita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbarnkob <@t> gmail.com Fri Mar 11 09:09:04 2011 From: mbarnkob <@t> gmail.com (Mike Stein Barnkob) Date: Fri Mar 11 09:09:10 2011 Subject: [Histonet] Double staining with x-gal and TCR-beta Message-ID: Dear Histonetters, I am trying to do a sequential double staining against beta-galactosidase and leukocytes in frozen mouse prostate tissue and have had little luck so fare; when I stain for x-gal or TCR-beta alone, they show up fine, but combined the protocols only the x-gal shows up. What I have been doing is this: Airdry for 5 min Fix slides in cold acetone for 10 min Wash slides 3x5 min in PBS Add x-gal Incubate overnight in humid chamber in the dark Wash in pbs for 5 min Incubate with blocking serum for 20 min Wash slides 5 min in wash buffer (10 mM sodium phosphate, pH 7.5, 0.9% PBS) Incubate with primary biotin-antibody overnight at room temperature Wash as before Incubate with ABC for 30 min Wash as before Incubate with DAB for 5 min Wash in tap water Mount Any suggestions would be very welcome - is this even possible or should I try something else? Thanks, Mike Barnkob From sfeher <@t> CMC-NH.ORG Fri Mar 11 09:33:00 2011 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Mar 11 09:33:05 2011 Subject: [Histonet] How many tissues an histotech is suppose to cut perhour? In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7406@EXCHMBC2.ad.ah.local> References: <000601cbd817$a4a252e0$ede6f8a0$@com>, <38667E7FB77ECD4E91BFAEB8D986386323DE133D95@LRGHEXVS1.practice.lrgh.org> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7406@EXCHMBC2.ad.ah.local> Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC24669C0B@exchange.cmc-nh.org> My hospital, like most, use benchmarking when examining how many people are needed to adequately provide services in any given area. Benchmarking data is widely available for most clinical lab scenarios but can be a bit lacking when it comes to pathology laboratories. I agree that the number of blocks per hour a histotech can cut must be examined in relation to the quality of those blocks. If I am anticipating an increase in workload and want to justify hiring more techs, it is invaluable to be able to cite national statistics, how my lab compares with them currently, and what I would need if my anticipated block count is anticipated to go up by a few thousand. Given that the information will be used to justify existing or future positions, would it be a fair statement that nationally, most histotechs average 24 blocks per hour (based on Jan's formula)? Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, March 01, 2011 10:02 AM To: 'Marcum, Pamela A'; Podawiltz, Thomas; 'Konop, Nicole'; histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut perhour? Well said Pam. All the speed in the world means nothing if you can't produce quality slides. The suggestion to do things the same way every time is a good one. However, learning what works well on what tissue is very helpful too. Like icing something that wrinkles or soaking something that shatters, etc. It all comes from experience. I plan to retire in 2 months but will keep my foot in the door working casual so that I don't lose my skills. I love this profession! Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 01, 2011 8:58 AM To: Podawiltz, Thomas; 'Konop, Nicole'; histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Tom. Janice is correct on her timing numbers. However; when you are starting out it is best to get the flow for working at your station than beating a clock. You will gain your speed with learning accuracy which is more important for the patient at the end. We are in patient care not NASCAR and should always remember patient first, which translates to accuracy and the best sections we can produce on every slide. Speed will come as you do more blocks and get better. Pam Marcum ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas [tpodawiltz@lrgh.org] Sent: Tuesday, March 01, 2011 8:26 AM To: 'Konop, Nicole'; histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Worry more about quality than speed at this time. I measure my staff on quality rather than how fast things get done. It's a waste of time, energy and resources to have to re-cut blocks because of poor quality. Speed comes with time and experience. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, March 01, 2011 9:14 AM To: histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Cynthia. Practice and setting up a standard system of how you cut. I do the same thing every time with each block I cut. Cutting faster is not necessarily a good thing if you are jeopardizing quality just to cut more slides. It's quality over quantity in my book any day! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, March 01, 2011 7:51 AM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Practice, practice, practice! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine h&e slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.....tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From traczyk7 <@t> aol.com Fri Mar 11 09:38:56 2011 From: traczyk7 <@t> aol.com (traczyk7@aol.com) Date: Fri Mar 11 09:39:06 2011 Subject: [Histonet] Warthin Starry Stain Message-ID: <8CDAE199111BF28-F14-C116@webmail-m094.sysops.aol.com> Greetings! Our usual reference lab does not do a spirochete stain. I would appreciate contact information for a lab that can do a Warthin Starry on FFPE tissue. Thanks. Dorothy From dchihc <@t> yahoo.com Fri Mar 11 09:54:29 2011 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Fri Mar 11 09:54:34 2011 Subject: [Histonet] p16ink4a Message-ID: <283226.6339.qm@web43509.mail.sp1.yahoo.com> Hi All, ?Is anyone using this?P16INK4A ?for IHC? ?I searched the archives and found some threads from 2009 with links ?that do not work for possible suppliers. I also found some for ELISA and Western Blot, and Flow Cytometry.?Is this antibody used at all in FFPE tissue? ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From ccrowder <@t> vetmed.lsu.edu Fri Mar 11 10:27:39 2011 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Fri Mar 11 10:31:09 2011 Subject: [Histonet] Fwd: GMS counterstain Message-ID: Ally - I have worked with several pathologists who were color-blind -one to greens; one to blues. As a result we counterstained the GMS with different colors. A counterstain is just that. It gives a color to secondary tissue elements. Light greens, hematoxylin, metanil yellow and nuclear fast red were used. All worked well, photographed well and were the choice of the pathologist. Many older pathologists "grew up" with the procedure outlined in the AFIP manual which stated green. But other pathologists are more amiable to trying other colors. Maybe you should just as them what they prefer. Cheryl From sfeher <@t> CMC-NH.ORG Fri Mar 11 10:43:28 2011 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Fri Mar 11 10:43:32 2011 Subject: [Histonet] Competancies for handling hazardous material In-Reply-To: References: <000601cbd3c0$d7ebaf80$87c30e80$@grics.net>, Message-ID: <0908FC0A43B87A4FB60EDCCA06AABC24669C13@exchange.cmc-nh.org> This is an early draft copy of what we are working on. Feel free to use it as a template and add any unique duties or items you need to. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fortin, Joyce Sent: Wednesday, March 02, 2011 1:21 PM To: Liz Chlipala; Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material Could I PLEASE have this information, too? I would really appreciate it. Joyce Fortin Histology Supervisor Palmdale Regional Medical Center 38600 Medical Center Drive Palmdale, California 93551 Phone 661-382-5723 Fax 661-382-5747 email: joyce.fortin@uhsinc.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala [liz@premierlab.com] Sent: Wednesday, February 23, 2011 6:51 PM To: Rae Staskiewicz; Histonet Subject: RE: [Histonet] Competancies for handling hazardous material Rae We have, you need standard training, but the individuals who inspected our lab did not mention compentances. But our inspection happened because we needed to switch from a conditionally exempt small quantity generator to a small quantitiy generator and we then needed to register with the state, etc. They primarily focused on our waste streams and what chemicals were in the lab. I'm not at work right now, but I'll check tomorrow to see if I have anything that I can share with you. Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rae Staskiewicz Sent: Wed 2/23/2011 6:19 PM To: Histonet Subject: [Histonet] Competancies for handling hazardous material Has anyone been inspected by the EPA regarding removal of hazardous chemicals? It came up in a safety meeting that I should have training and competencies (beyond general safety training and PPE) for pouring xylene from staining dishes and processors into accumulation containers. Rae Ann Staskiewicz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Fri Mar 11 10:45:57 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Mar 11 10:46:01 2011 Subject: [Histonet] p16ink4a In-Reply-To: <283226.6339.qm@web43509.mail.sp1.yahoo.com> References: <283226.6339.qm@web43509.mail.sp1.yahoo.com> Message-ID: Go to www.mtmlabs.com. They have the patent. Vikki On Mar 11, 2011 10:55 AM, "Phyllis Thaxton" wrote: > Hi All, > > Is anyone using this P16INK4A for IHC? I searched the archives and found some > threads from 2009 with links that do not work for possible suppliers. I also > found some for ELISA and Western Blot, and Flow Cytometry. Is this antibody used > at all in FFPE tissue? > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From COPPINM <@t> aruplab.com Fri Mar 11 10:54:13 2011 From: COPPINM <@t> aruplab.com (Coppin, Margaret) Date: Fri Mar 11 10:54:20 2011 Subject: [Histonet] Her2 Gastric cases Message-ID: Hello, I am hoping someone can shed some light on whether or not Her2 4B5 (Ventana's antibody) is okay to run on gastric biopsies. We are getting some client requests for this and I wonder if I should re-direct them toward the Dako Hercep Test instead. Thank you. Margaret G. Coppin, HT(ASCP) Technical Supervisor--Immunohistochemistry ARUP Laboratories 500 Chipeta Way Salt Lake City, UT 84108 (801)583-2787 X3869 coppinm@ARUPLab.com - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From liz <@t> premierlab.com Fri Mar 11 10:59:46 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Mar 11 10:59:49 2011 Subject: [Histonet] Her2 Gastric cases In-Reply-To: Message-ID: Margaret Dako has a bunch of info on Her2 staining in gastric cancer its in the most recent Connection Volume 15 and they also have a companion document that covers Her 2 staining and scoring for gastric cancer, just go to the Dako website and you should be able to view these documents. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Coppin, Margaret Sent: Friday, March 11, 2011 9:54 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Her2 Gastric cases Hello, I am hoping someone can shed some light on whether or not Her2 4B5 (Ventana's antibody) is okay to run on gastric biopsies. We are getting some client requests for this and I wonder if I should re-direct them toward the Dako Hercep Test instead. Thank you. Margaret G. Coppin, HT(ASCP) Technical Supervisor--Immunohistochemistry ARUP Laboratories 500 Chipeta Way Salt Lake City, UT 84108 (801)583-2787 X3869 coppinm@ARUPLab.com - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Mar 11 11:03:02 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Mar 11 11:13:00 2011 Subject: [Histonet] Her2 Gastric cases In-Reply-To: References: Message-ID: We do use the Ventana antibody for gastric cases. There is some difference in reading the slide for the pathologist. I'll send you an article on it in a seperate e-mail (so I can attach it). Mark On Fri, Mar 11, 2011 at 8:54 AM, Coppin, Margaret wrote: > Hello, > > > > I am hoping someone can shed some light on whether or not Her2 4B5 > (Ventana's antibody) is okay to run on gastric biopsies. We are getting > some client requests for this and I wonder if I should re-direct them > toward the Dako Hercep Test instead. > > > > Thank you. > > > > Margaret G. Coppin, HT(ASCP) > > Technical Supervisor--Immunohistochemistry > > > > ARUP Laboratories > > 500 Chipeta Way > > Salt Lake City, UT 84108 > > (801)583-2787 X3869 > > coppinm@ARUPLab.com > > > > > > > > > - ------------------------------------------------------------------ > The information transmitted by this e-mail and any included > attachments are from ARUP Laboratories and are intended only for the > recipient. The information contained in this message is confidential > and may constitute inside or non-public information under > international, federal, or state securities laws, or protected health > information and is intended only for the use of the recipient. > Unauthorized forwarding, printing, copying, distributing, or use of > such information is strictly prohibited and may be unlawful. If you > are not the intended recipient, please promptly delete this e-mail > and notify the sender of the delivery error or you may call ARUP > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 > (800) 522-2787 ext. 2100 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JEllin <@t> yumaregional.org Fri Mar 11 11:16:36 2011 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Mar 11 11:16:46 2011 Subject: [Histonet] Her2 Gastric cases In-Reply-To: References: Message-ID: <29BE166A2CF48D459853F8EC57CD37E8035D6A00@EXCHANGECLUSTER.yumaregional.local> Mark can you send me the information as well, Thanks -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, March 11, 2011 10:03 AM To: Coppin, Margaret Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Her2 Gastric cases We do use the Ventana antibody for gastric cases. There is some difference in reading the slide for the pathologist. I'll send you an article on it in a seperate e-mail (so I can attach it). Mark On Fri, Mar 11, 2011 at 8:54 AM, Coppin, Margaret wrote: > Hello, > > > > I am hoping someone can shed some light on whether or not Her2 4B5 > (Ventana's antibody) is okay to run on gastric biopsies. We are getting > some client requests for this and I wonder if I should re-direct them > toward the Dako Hercep Test instead. > > > > Thank you. > > > > Margaret G. Coppin, HT(ASCP) > > Technical Supervisor--Immunohistochemistry > > > > ARUP Laboratories > > 500 Chipeta Way > > Salt Lake City, UT 84108 > > (801)583-2787 X3869 > > coppinm@ARUPLab.com > > > > > > > > > - ------------------------------------------------------------------ > The information transmitted by this e-mail and any included > attachments are from ARUP Laboratories and are intended only for the > recipient. The information contained in this message is confidential > and may constitute inside or non-public information under > international, federal, or state securities laws, or protected health > information and is intended only for the use of the recipient. > Unauthorized forwarding, printing, copying, distributing, or use of > such information is strictly prohibited and may be unlawful. If you > are not the intended recipient, please promptly delete this e-mail > and notify the sender of the delivery error or you may call ARUP > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 > (800) 522-2787 ext. 2100 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From mlm11 <@t> cornell.edu Fri Mar 11 14:19:10 2011 From: mlm11 <@t> cornell.edu (Mary Lou Norman) Date: Fri Mar 11 14:19:16 2011 Subject: [Histonet] equine bone decal Message-ID: <4010584DE26D90419367C0A5F620E9633027F1C424@MBXB.exchange.cornell.edu> Hi, We've been using EDTA to decal and of course it takes forever. I would love to hear what others are using for large bones, joints, etc. We do not work on rats or mice, just horses. Thanks. Many prayers to Japan and beyond. Mary Lou From JWeems <@t> sjha.org Fri Mar 11 15:39:04 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Mar 11 15:39:08 2011 Subject: [Histonet] FW: Slides to Pathologist Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081E21A08E@CHEXCMS10.one.ads.che.org> I received this question from my Quality Manager today. Hi Joyce, What do you consider good performance regarding this? I need some sort of goal for our lab key control points chart. I could only find one reference on the internet from Presbyterian Hospital in Charlotte, NC from 1993 (yuck). Their goal was 90% of the slides on time which they correlated to 2 days or less late slides per month. Their goal was also to have all the slides out by 11am...... Could I get feedback from you guys about what you expect for this PI goal? I'd like 100%, but sometimes staffing just doesn't stretch far enough!! Thanks, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From lucy.zong <@t> gmail.com Fri Mar 11 16:26:50 2011 From: lucy.zong <@t> gmail.com (Lucy Zong) Date: Fri Mar 11 16:26:56 2011 Subject: [Histonet] Leica TP1020 Message-ID: loking for this processor From flnails <@t> texaschildrens.org Fri Mar 11 16:37:43 2011 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Mar 11 16:37:50 2011 Subject: [Histonet] RE: Slides to Pathologist In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164081E21A08E@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E164081E21A08E@CHEXCMS10.one.ads.che.org> Message-ID: 100% is unrealistic unless your staff never calls in sick, take vacations, your lab never gets research projects that are needed today and you are always fully staffed. Realistic reside between 90 and 95% which is obtainable but not overly easily obtainable -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, March 11, 2011 3:39 PM To: Histonet Subject: [Histonet] FW: Slides to Pathologist I received this question from my Quality Manager today. Hi Joyce, What do you consider good performance regarding this? I need some sort of goal for our lab key control points chart. I could only find one reference on the internet from Presbyterian Hospital in Charlotte, NC from 1993 (yuck). Their goal was 90% of the slides on time which they correlated to 2 days or less late slides per month. Their goal was also to have all the slides out by 11am...... Could I get feedback from you guys about what you expect for this PI goal? I'd like 100%, but sometimes staffing just doesn't stretch far enough!! Thanks, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== From kfraka <@t> hotmail.com Fri Mar 11 16:54:54 2011 From: kfraka <@t> hotmail.com (Katrena Fraka) Date: Fri Mar 11 16:54:58 2011 Subject: [Histonet] (no subject) Message-ID: Are there any Histo Techs in the Stockton, CA area looking for a full time position? From tkngflght <@t> yahoo.com Fri Mar 11 22:02:14 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Mar 11 22:02:17 2011 Subject: [Histonet] temp needed asap Message-ID: <502922.20753.qm@web39420.mail.mud.yahoo.com> Looking for an experienced temp ASAP.? Warm weather, daytime hours, NICE city, 4-6 weeks. ? If you have several assignments under your belt and are ready for a new location, respond with a FULL RESUME attached to your email.? We cannot?call on?anything less than your resume for this one. ? We do not submit resumes without your consent.? (I didn't like it when it was done?to me!) ? Cheryl ? admin@fullstaff.org From thomas6093 <@t> yahoo.com Sat Mar 12 12:51:30 2011 From: thomas6093 <@t> yahoo.com (Thomas Huynh) Date: Sat Mar 12 12:51:39 2011 Subject: [Histonet] bone decal Message-ID: <327910.66086.qm@web80405.mail.mud.yahoo.com> Hi Mary We use 10% Formic Acid for our bone marrow bx and larger surgical bones (Ex: femur, pel-vis, mandibular bones) Thomas Huynh ? ________________________________ From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Sat, March 12, 2011 12:01:57 PM Subject: Histonet Digest, Vol 88, Issue 15 Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. equine bone decal (Mary Lou Norman) ? 2. FW: Slides to Pathologist (Weems, Joyce) ? 3. Leica TP1020 (Lucy Zong) ? 4. RE: Slides to Pathologist (Nails, Felton) ? 5. (no subject) (Katrena Fraka) ? 6. temp needed asap (Cheryl) ---------------------------------------------------------------------- Message: 1 Date: Fri, 11 Mar 2011 15:19:10 -0500 From: Mary Lou Norman Subject: [Histonet] equine bone decal To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <4010584DE26D90419367C0A5F620E9633027F1C424@MBXB.exchange.cornell.edu> Content-Type: text/plain; charset="us-ascii" Hi, We've been using EDTA to decal and of course it takes forever. I would love to hear what others are using for large bones, joints, etc. We do not work on rats or mice, just horses. Thanks. Many prayers to Japan and beyond. Mary Lou ------------------------------ Message: 2 Date: Fri, 11 Mar 2011 16:39:04 -0500 From: "Weems, Joyce" Subject: [Histonet] FW: Slides to Pathologist To: Histonet Message-ID: ??? <92AD9B20A6C38C4587A9FEBE3A30E164081E21A08E@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I received this question from my Quality Manager today. Hi Joyce, What do you consider good performance regarding this?? I need some sort of goal for our lab key control points chart. I could only find one reference on the internet from Presbyterian Hospital in Charlotte, NC from 1993 (yuck).? Their goal was 90% of the slides on time which they correlated to 2 days or less late slides per month. Their goal was also to have all the slides out by 11am...... Could I get feedback from you guys about what you expect for this PI goal? I'd like 100%, but sometimes staffing just doesn't stretch far enough!! Thanks, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 3 Date: Fri, 11 Mar 2011 17:26:50 -0500 From: Lucy Zong Subject: [Histonet] Leica TP1020 To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 loking for this processor ------------------------------ Message: 4 Date: Fri, 11 Mar 2011 16:37:43 -0600 From: "Nails, Felton" Subject: [Histonet] RE: Slides to Pathologist To: "'Weems, Joyce'" ,??? "Histonet" ??? Message-ID: ??? ??? Content-Type: text/plain; charset=us-ascii 100% is unrealistic unless your staff never calls in sick, take vacations, your lab never gets research projects that are needed today and you are always fully staffed. Realistic reside between 90 and 95% which is obtainable but not overly easily obtainable -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, March 11, 2011 3:39 PM To: Histonet Subject: [Histonet] FW: Slides to Pathologist I received this question from my Quality Manager today. Hi Joyce, What do you consider good performance regarding this?? I need some sort of goal for our lab key control points chart. I could only find one reference on the internet from Presbyterian Hospital in Charlotte, NC from 1993 (yuck).? Their goal was 90% of the slides on time which they correlated to 2 days or less late slides per month. Their goal was also to have all the slides out by 11am...... Could I get feedback from you guys about what you expect for this PI goal? I'd like 100%, but sometimes staffing just doesn't stretch far enough!! Thanks, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ============================================================================== ------------------------------ Message: 5 Date: Fri, 11 Mar 2011 14:54:54 -0800 From: Katrena Fraka Subject: [Histonet] (no subject) To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Are there any Histo Techs in the Stockton, CA area looking for a full time position? ? ??? ??? ??? ? ??? ??? ? ------------------------------ Message: 6 Date: Fri, 11 Mar 2011 20:02:14 -0800 (PST) From: Cheryl Subject: [Histonet] temp needed asap To: histonet@lists.utsouthwestern.edu Message-ID: <502922.20753.qm@web39420.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Looking for an experienced temp ASAP.? Warm weather, daytime hours, NICE city, 4-6 weeks. ? If you have several assignments under your belt and are ready for a new location, respond with a FULL RESUME attached to your email.? We cannot?call on?anything less than your resume for this one. ? We do not submit resumes without your consent.? (I didn't like it when it was done?to me!) ? Cheryl ? admin@fullstaff.org ? ? ? ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 88, Issue 15 **************************************** From tkngflght <@t> yahoo.com Sat Mar 12 15:56:12 2011 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sat Mar 12 15:56:16 2011 Subject: [Histonet] slides to pathologist - one response. Message-ID: <698061.5378.qm@web39405.mail.mud.yahoo.com> Joyce- ? Every lab is different and it doesn't just depend on the histotechs.? Are there reprocessing issues, do you have decals, held for fixation, specials, IHCs, other delays inherent with the process.? Are you counting 'slides on the desk' or 'cases signed out and released to physicians'?? If the latter, then you have another dimension for metrics.? Add in?cases delayed?for internal? or external consults and other special testing,(unless they're signed out with later?addendums--yuck!) and other reasonable delays.??And yes, there are staffing issues, equipment issues, and all the other 'issues'.? In reality--there is no universal standard other than a few gross similarities. ? Most labs?use the afore-mentioned 90-95% by 2nd day post receipt (not post procurement unless you're working in a hospital with controlled distrubution).? For your lab--start with something loose with measurables?(metrics) that are concrete.? Don't make it too detailed or you'll spend your day chasing numbers!? ? Once you start a program, keep track.over a?short period of time to tighten things?your metrics?and make sure what your measuring is real and has meaning.? Measure and develop categories for reasonable delays that you won't count against turn-around-time (TAT)?(ex: we pull out decals).??We don't want to set up systems that cause more 'aw shucks' moments artifically because we didn't set the measured components in line with reality.? I've?worked in this kind of situation--amazingly demoralizing because the staff can't win their 'game of Slides On The Desk'? and TAT can even get worse if your metrics aren't attainable!? ?Fortunately, the inverse is also true!! ? my three cents-- ? Cheryl K? From dbeil1 <@t> hotmail.com Sun Mar 13 10:04:48 2011 From: dbeil1 <@t> hotmail.com (Damaris Beil) Date: Sun Mar 13 10:04:52 2011 Subject: [Histonet] Competency Checklist Message-ID: Does anyone have a competency checklist they would not mind sharing? If you would like to share yours, please e-mail to dbeil1@hotmail.com. This would be for Technicians/Technologists. Thanks In Advance, Damaris E. Beil, HT,ASCPcm, HTL Associates in Dermatology Histology Laboratory (407) 846-7546 ext. 3307 From tahseen <@t> brain.net.pk Sun Mar 13 22:27:51 2011 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Sun Mar 13 22:28:03 2011 Subject: [Histonet] Protocol for manual processing Message-ID: <43142.203.135.35.66.1300073271.squirrel@brain.net.pk> Does anyone have a protocol for manual processing for mouse kidneys (ethanol substitute? Thanks In Advance, Muhammad Tahseen SKMCH&RC Lahore Pakistan From Michel.Bataillon <@t> citox.com Mon Mar 14 04:53:42 2011 From: Michel.Bataillon <@t> citox.com (Bataillon Michel) Date: Mon Mar 14 04:53:50 2011 Subject: [Histonet] g ratio Message-ID: <1FB9362B907EEC43959F72445AC63B637CFEA3@apollon.cit_serveur.com> Hello, We have to measure the "g ratio" (ratio of the inner axonal diameter to the total outer diameter) - mice sciatic nerve - resin sectioning / toluidine blue - at least 500 myelinated fibers per nerve will be evaluated with a standard imaging software. Do you think that we can measure the number of demyelinated axons without perform any additionnal stainings? Thank you, Michel Michel BATAILLON Histology Evreux, France ************************************************************* Ce message et toutes les pieces jointes sont confidentiels et etablis a l'attention exclusive de ses destinataires. Si vous recevez ce message par erreur, merci de le detruire et d'en avertir immediatement l'expediteur. Toute utilisation de ce message non conforme a sa destination, toute diffusion ou toute publication, totale ou partielle, est interdite. Internet ne permettant pas de garantir l'integrite de ce message, le CIT decline toute responsabilite au titre de ce message s'il a ete altere, deforme ou falsifie. Protegeons ensemble l'environnement, n'imprimons ce mail (et ses pieces jointes) que si cela est necessaire. This transmission and any attachments are confidential and intended solely for the use of the addressee(s). If you are not an intended recipient, please notify us immediately by replying to the message and deleting it from your computer. In this case, any unauthorized use, copying or distribution is strictly prohibited. Please be aware that E-mails are susceptible to alteration and their integrity cannot be guaranteed. CIT shall not be liable for this E-mail if corrupted, changed or falsified. Please consider the environment before printing this E-mail. ************************************************************* From David.Muskett <@t> elht.nhs.uk Mon Mar 14 05:01:08 2011 From: David.Muskett <@t> elht.nhs.uk (Muskett David (ELHT) Pathology) Date: Mon Mar 14 05:01:20 2011 Subject: [Histonet] RE: Histonet Digest, Vol 88, Issue 16 In-Reply-To: <201103131701.AJG48977@rlmh11.swi.contact.secure-ops.net> Message-ID: Here is the latest document from the UK on productivity. http://system.improvement.nhs.uk/ImprovementSystem/ViewDocument.aspx?path=Diagnostics%2fNational%2fWebsite%2fHistology%20Guide%202.pdf _____ David Muskett Chief Biomedical Scientist, Histology East Lancashire Hospitals NHS Trust | Royal Blackburn Hospital | Haslingden Road, Blackburn | BB2 3HH david.muskett@elht.nhs.uk | www.pathologyineastlancs.org.uk Telephone x 82438 | 01254 732438 | Fax 01254 736081 |Histology results 01254 732621 or internal x82621 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 13 March 2011 17:02 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 88, Issue 16 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. bone decal (Thomas Huynh) 2. slides to pathologist - one response. (Cheryl) 3. Competency Checklist (Damaris Beil) ---------------------------------------------------------------------- Message: 1 Date: Sat, 12 Mar 2011 10:51:30 -0800 (PST) From: Thomas Huynh Subject: [Histonet] bone decal To: histonet@lists.utsouthwestern.edu Message-ID: <327910.66086.qm@web80405.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Mary We use 10% Formic Acid for our bone marrow bx and larger surgical bones (Ex: femur, pel-vis, mandibular bones) Thomas Huynh ? ________________________________ From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Sat, March 12, 2011 12:01:57 PM Subject: Histonet Digest, Vol 88, Issue 15 Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. equine bone decal (Mary Lou Norman) ? 2. FW: Slides to Pathologist (Weems, Joyce) ? 3. Leica TP1020 (Lucy Zong) ? 4. RE: Slides to Pathologist (Nails, Felton) ? 5. (no subject) (Katrena Fraka) ? 6. temp needed asap (Cheryl) ---------------------------------------------------------------------- Message: 1 Date: Fri, 11 Mar 2011 15:19:10 -0500 From: Mary Lou Norman Subject: [Histonet] equine bone decal To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <4010584DE26D90419367C0A5F620E9633027F1C424@MBXB.exchange.cornell.edu> Content-Type: text/plain; charset="us-ascii" Hi, We've been using EDTA to decal and of course it takes forever. I would love to hear what others are using for large bones, joints, etc. We do not work on rats or mice, just horses. Thanks. Many prayers to Japan and beyond. Mary Lou ------------------------------ Message: 2 Date: Fri, 11 Mar 2011 16:39:04 -0500 From: "Weems, Joyce" Subject: [Histonet] FW: Slides to Pathologist To: Histonet Message-ID: ??? <92AD9B20A6C38C4587A9FEBE3A30E164081E21A08E@CHEXCMS10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I received this question from my Quality Manager today. Hi Joyce, What do you consider good performance regarding this?? I need some sort of goal for our lab key control points chart. I could only find one reference on the internet from Presbyterian Hospital in Charlotte, NC from 1993 (yuck).? Their goal was 90% of the slides on time which they correlated to 2 days or less late slides per month. Their goal was also to have all the slides out by 11am...... Could I get feedback from you guys about what you expect for this PI goal? I'd like 100%, but sometimes staffing just doesn't stretch far enough!! Thanks, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 3 Date: Fri, 11 Mar 2011 17:26:50 -0500 From: Lucy Zong Subject: [Histonet] Leica TP1020 To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 loking for this processor ------------------------------ Message: 4 Date: Fri, 11 Mar 2011 16:37:43 -0600 From: "Nails, Felton" Subject: [Histonet] RE: Slides to Pathologist To: "'Weems, Joyce'" ,??? "Histonet" ??? Message-ID: ??? ??? Content-Type: text/plain; charset=us-ascii 100% is unrealistic unless your staff never calls in sick, take vacations, your lab never gets research projects that are needed today and you are always fully staffed. Realistic reside between 90 and 95% which is obtainable but not overly easily obtainable -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, March 11, 2011 3:39 PM To: Histonet Subject: [Histonet] FW: Slides to Pathologist I received this question from my Quality Manager today. Hi Joyce, What do you consider good performance regarding this?? I need some sort of goal for our lab key control points chart. I could only find one reference on the internet from Presbyterian Hospital in Charlotte, NC from 1993 (yuck).? Their goal was 90% of the slides on time which they correlated to 2 days or less late slides per month. Their goal was also to have all the slides out by 11am...... Could I get feedback from you guys about what you expect for this PI goal? I'd like 100%, but sometimes staffing just doesn't stretch far enough!! Thanks, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged.? If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system.? Thank you. ============================================================================== ------------------------------ Message: 5 Date: Fri, 11 Mar 2011 14:54:54 -0800 From: Katrena Fraka Subject: [Histonet] (no subject) To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Are there any Histo Techs in the Stockton, CA area looking for a full time position? ? ??? ??? ??? ? ??? ??? ? ------------------------------ Message: 6 Date: Fri, 11 Mar 2011 20:02:14 -0800 (PST) From: Cheryl Subject: [Histonet] temp needed asap To: histonet@lists.utsouthwestern.edu Message-ID: <502922.20753.qm@web39420.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Looking for an experienced temp ASAP.? Warm weather, daytime hours, NICE city, 4-6 weeks. ? If you have several assignments under your belt and are ready for a new location, respond with a FULL RESUME attached to your email.? We cannot?call on?anything less than your resume for this one. ? We do not submit resumes without your consent.? (I didn't like it when it was done?to me!) ? Cheryl ? admin@fullstaff.org ? ? ? ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 88, Issue 15 **************************************** ------------------------------ Message: 2 Date: Sat, 12 Mar 2011 13:56:12 -0800 (PST) From: Cheryl Subject: [Histonet] slides to pathologist - one response. To: histonet@lists.utsouthwestern.edu Message-ID: <698061.5378.qm@web39405.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Joyce- ? Every lab is different and it doesn't just depend on the histotechs.? Are there reprocessing issues, do you have decals, held for fixation, specials, IHCs, other delays inherent with the process.? Are you counting 'slides on the desk' or 'cases signed out and released to physicians'?? If the latter, then you have another dimension for metrics.? Add in?cases delayed?for internal? or external consults and other special testing,(unless they're signed out with later?addendums--yuck!) and other reasonable delays.??And yes, there are staffing issues, equipment issues, and all the other 'issues'.? In reality--there is no universal standard other than a few gross similarities. ? Most labs?use the afore-mentioned 90-95% by 2nd day post receipt (not post procurement unless you're working in a hospital with controlled distrubution).? For your lab--start with something loose with measurables?(metrics) that are concrete.? Don't make it too detailed or you'll spend your day chasing numbers!? ? Once you start a program, keep track.over a?short period of time to tighten things?your metrics?and make sure what your measuring is real and has meaning.? Measure and develop categories for reasonable delays that you won't count against turn-around-time (TAT)?(ex: we pull out decals).??We don't want to set up systems that cause more 'aw shucks' moments artifically because we didn't set the measured components in line with reality.? I've?worked in this kind of situation--amazingly demoralizing because the staff can't win their 'game of Slides On The Desk'? and TAT can even get worse if your metrics aren't attainable!? ?Fortunately, the inverse is also true!! ? my three cents-- ? Cheryl K? ------------------------------ Message: 3 Date: Sun, 13 Mar 2011 11:04:48 -0400 From: Damaris Beil Subject: [Histonet] Competency Checklist To: histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Does anyone have a competency checklist they would not mind sharing? If you would like to share yours, please e-mail to dbeil1@hotmail.com. This would be for Technicians/Technologists. Thanks In Advance, Damaris E. Beil, HT,ASCPcm, HTL Associates in Dermatology Histology Laboratory (407) 846-7546 ext. 3307 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 88, Issue 16 **************************************** From sbreeden <@t> nmda.nmsu.edu Mon Mar 14 07:59:30 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Mar 14 07:59:35 2011 Subject: [Histonet] Daylight Savings Time? Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E476FD@nmdamailsvr.nmda.ad.nmsu.edu> Does anyone know the OLD daylight savings time date that USED to be in effect? I'll have to manually change the processor time to avoid being late (or early - who knows?). Today, I was way early! Senior moment, I suppose... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From b-frederick <@t> northwestern.edu Mon Mar 14 11:28:28 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Mar 14 11:28:34 2011 Subject: [Histonet] Daylight Savings Time? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E476FD@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <77D9CC5BE1034D63B6061446FFBDC676@lurie.northwestern.edu> I think it used to be the first weekend in April,but I was also told it may have been this coming weekend (in Europe anyway) Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, March 14, 2011 8:00 AM To: Histonet Subject: [Histonet] Daylight Savings Time? Does anyone know the OLD daylight savings time date that USED to be in effect? I'll have to manually change the processor time to avoid being late (or early - who knows?). Today, I was way early! Senior moment, I suppose... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Farnana <@t> nehealth.com Mon Mar 14 11:35:53 2011 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Mon Mar 14 11:36:15 2011 Subject: [Histonet] New York State Histotechnology Annual Spring Symposium Program Message-ID: <4D7E0BA8.26ED.00D9.1@nehealth.com> NYSHS ANNUAL SPRING SYMPOSIUM ?SLIDING INTO THE FUTURE? May 14, 2011 Desmond Hotel and Conference Center Albany, NY The NYSHS has planned a wonderful program for this spring allowing its members to receive 6 CEU?s of credit in one day! You will be receiving your programs in the mail very soon but we would like to give you the meeting overview for those that need to submit for funding. Registration opens at 7:00am and the first session is at 8:00am. Program and registration will be posted on the NYSHS website www.nyhisto.org ( http://www.nyhisto.org/ )soon. 1) Virgil Hernadez CT (ASCP) Title: Digital Pathology Specialist, Ventana Medical Systems Talk Title: Digital Pathology 101 Abstract:This course will introduce meeting participants to the exciting new world of Digital Pathology. This 1 hour presentation will provide broad overview of basic system hardware and software required for converting prepared slides to whole slide images. Participants will become familiar with key applications of Digital Pathology which include: Telepathology, teleconsultation, web conferencing, IHC analysis, tumor board, and robotic microscopy. Current market trends in Digital Pathology adoption and products will be discussed. 2) Dr. Kari Reiber Title: Chief Medical Examiner, Dutchess County, NY. Dutchess County Department of Health Talk Title: The role of Histopathology in Forensic Postmortem Investigations Abstract: The ever increasing popularity of crime fiction has done little to improve the public?s understanding of forensic pathology. Fictional dramatizations focus on the forensic sciences rather than on forensic pathology, and often confuse the two. The popularity of ?forensics? is having a positive effect, in that many young people are opting for a career in science. Unfortunately the so-called ?CSI effect? is having a negative impact in the courtroom as a result of the unrealistic expectations of some jury members. Fictional medical examiners have many unrealistic identities and are portrayed as gun-toting vigilantes, forensic technology wizards, glamorous law enforcement officers, or cranky eccentrics, but almost never with their one essential instrument: the microscope. One forgets that forensic pathologists are actually pathologists specialized in the anatomy of injury and injury patterns. When investigating sudden, unexplained, and violent deaths, the forensic pathologist is mandated by law to perform a postmortem examination which generally consists of an autopsy. A complete forensic autopsy usually requires an external examination, an internal examination, a microscopic examination, and a comprehensive toxicological analysis. The purpose of this presentation is to define forensic pathology and forensic science, to clarify the actual role of the forensic pathologist and to illustrate by way of specific examples the crucial role of histopathology in forensic postmortem investigations. 3) Joseph Dudek, M.D. , US Oncology Incorporated ? New York Oncology Hematology. Talk Title: Personalized Approach for Non-Small Cell Lung Cancer Abstract: The treatment of non-small cell lung cancer will be reviewed and a discussion of how systemic treatment is determined based on the histology and stage of the tumor. The importance of adenocarcinoma, squamous cell carcinoma and NOS will be discussed in regard to first line and second line systemic treatments. There are definite differences in regard to the choice of chemotherapy and its effectiveness in squamous and non-squamous histologies. We will also discuss EGFR mutations and there can influence on choice of systemic therapy for non-small cell lung cancer. Tyrosine kinase Inhibitors provide an alternative systemic treatment for patients with EGFR mutations. Toxicities of the treatment will be reviewed. Lastly the EML4- ALK mutation will be reviewed and its influence on potential treatments will be discussed. 4) Valantou Grover, HT, HTL(ASCP), PA, MBA Title: Biosciences Product Line Manager, Polysciences, Inc. Talk Title: The Right Stain, Troubleshooting Histology Stains Abstract: When routine stains go wrong, pathologists return slides to the responsible department for restaining: histology (routine and special stains), cytology or hematology. The repeat staining process on the old and/or the new slides reduces the expected turnaround time. Processes exist far beyond the control of the technician/technologist, not related to their skill, technique, and/or experience. Inconsistent staining may occur because townships change additives in water supply systems or filtration processes, mistakes in manufacturing processes as simple as water temperature variance, market supply, market demand, quality of the raw materials, availability of raw materials, incorrect shipping department standards, and/or the environment. The presentation allows lab professionals to examine troubleshooting techniques considered ?outside the box? or scope of what is routine troubleshooting in the lab. Staff shortages, pressure on pathologists and lab professionals by clinicians, and/or specimen diagnosis quotas allow laboratory validation standards to be completed on a stain or protocol/process developed by another entity other than the end-user. Commercial batches are manufactured in such large quantities, that a reduction in lot to lot variation makes validation more accurate and allows for accurate reproducibility of the stain process, regardless of which end user is performing the staining process: the human and/or the analyzer. 5) Susan Ryan, HTL(ASCP), Genzyme, Inc. Talk Title: Hard, Harder and Hardest: Choosing the right process for your bone project Abstract: Processing tissue containing bone and cartilage has challenged histologists throughout the years. It is our responsibility to understand these challenges, know the tissue and cell components and provide the pathologist and/or investigator with quality stained slides. In recent years digital pathology has created new opportunities for routine labs to incorporate different processing methods for bone. In this workshop we will discuss decalcified (paraffin and frozen) and undecalcified (methyl methacrylate and Epon) methods for processing bone that best fits the diagnosis and or analysis. Detailed protocols will be presented pertaining to processing and staining of samples containing bone and cartilage. We will discuss the common problems with both methods and share some of the ?tricks of the trade?. We will focus on aiding pathologists and/or investigators with understanding diseases such as rheumatoid arthritis, osteoarthritis, renal osteodystrophy and osteoporosis. Upon completion participants will 1) identify tissue components and cells 2) identify which processing procedure best fits the diagnosis or analysis 3) identify the staining differences in each processing methods 4) identify artifacts in each method. 6) Amy Farnan, HT (ASCP) Title: Supervisor, Histology: Albany Memorial Hospital/Samaritan Hospital, North East Health Talk Title: Formalin Recycling: ?Is your lab safe?" Abstract: The recycler is installed; everyone has been trained on its use and its go time right? Wrong! How many of you that have a recycler had safety training before you started using the recycler? In this seminar learn what measures your lab should have in place to keep your employee?s safe and the histology laboratory regulatory compliant. Participating vendors to date: Neogenomics laboratories Tech One Biomedical Services Azer Scientific Electron Microscopy Sciences Source Medical Products Polysciences Leica Biosystems Sakura VWR International Newcomer Supply Poly Scientific Registration: 7:30 am to 10:00 am First session commences at 8:00 am Registration price includes: All sessions and lunch. $100.00 /member $135.00/non-member $60.00/ student Desmond room rates: $129.00/ night When booking your room reference group ID# 11O344 (O not a zero) Booking deadline is April 21, 2011 Desmond contact number: 1-800-448-3500 www.desmondhotelsalbany.com Hope to see you all there!! Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From leiker <@t> buffalo.edu Mon Mar 14 12:45:19 2011 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Mon Mar 14 12:45:38 2011 Subject: [Histonet] Daylight Savings Time? In-Reply-To: <77D9CC5BE1034D63B6061446FFBDC676@lurie.northwestern.edu> References: <77D9CC5BE1034D63B6061446FFBDC676@lurie.northwestern.edu> Message-ID: <7AE5A495677637FD57E70F05@CDYwxp1931.ad.med.buffalo.edu> Yes, it used to be April 2 (in the U.S.). I had to check an older calendar to be sure: --On Monday, March 14, 2011 11:28 AM -0500 Bernice Frederick wrote: > I think it used to be the first weekend in April,but I was also told it > may have been this coming weekend (in Europe anyway) > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, > Sara > Sent: Monday, March 14, 2011 8:00 AM > To: Histonet > Subject: [Histonet] Daylight Savings Time? > > Does anyone know the OLD daylight savings time date that USED to be in > effect? I'll have to manually change the processor time to avoid being > late (or early - who knows?). Today, I was way early! Senior moment, I > suppose... > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From tjay30 <@t> yahoo.com Mon Mar 14 12:56:13 2011 From: tjay30 <@t> yahoo.com (Timothy Jay) Date: Mon Mar 14 12:56:16 2011 Subject: [Histonet] Job Opening Message-ID: <536690.24031.qm@web34307.mail.mud.yahoo.com> We are still looking for a certified histotech to run a new in-office GI path lab in Santa Rosa, CA. Excellent pay and working conditions. Must have experience.?Hours are very flexible. No new grads (sorry). Send resume to tjay30@yahoo.com ? ? Tim From Vickroy.Jim <@t> mhsil.com Mon Mar 14 13:16:44 2011 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Mar 14 13:17:01 2011 Subject: [Histonet] VALIDATION of a new clone Message-ID: <24A4826E8EF0964D86BC5317306F58A5556A2EC5C6@mmc-mail.ad.mhsil.com> I am wondering what others are doing when they validate a new clone of a antibody that you have used in the lab for quite sometime. When we introduce a new antibody obviously we go through a pretty extensive validation process including sampling of around 20 - 30 specimens, some presumed positive cases and some presumed negative cases. We also try to include in the positive cases some that are strongly positive and others that are weakly positive. Lately several of our regular antibodies are now no longer available in the same clone. I am interested in how different labs would validate the new clone to be used in the lab. It would seem to me that the validation process would not have to be as extensive since we have already established that the antibody works well in our laboratory. I know if it was the same clone but a different lot we would just do a comparison of the old lot with the new lot, but what about a different clone? I realize that the methods are going to be diverse but I would appreciate hearing from others. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From liz <@t> premierlab.com Mon Mar 14 13:21:09 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Mar 14 13:21:34 2011 Subject: [Histonet] VALIDATION of a new clone In-Reply-To: <24A4826E8EF0964D86BC5317306F58A5556A2EC5C6@mmc-mail.ad.mhsil.com> Message-ID: If it's a different clone then it's essentially a different antibody you would have to go through the same process as you did initially. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, March 14, 2011 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VALIDATION of a new clone I am wondering what others are doing when they validate a new clone of a antibody that you have used in the lab for quite sometime. When we introduce a new antibody obviously we go through a pretty extensive validation process including sampling of around 20 - 30 specimens, some presumed positive cases and some presumed negative cases. We also try to include in the positive cases some that are strongly positive and others that are weakly positive. Lately several of our regular antibodies are now no longer available in the same clone. I am interested in how different labs would validate the new clone to be used in the lab. It would seem to me that the validation process would not have to be as extensive since we have already established that the antibody works well in our laboratory. I know if it was the same clone but a different lot we would just do a comparison of the old lot with the new lot, but what about a different clone? I realize that the methods are going to be diverse but I would appreciate hearing from others. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Mon Mar 14 13:21:50 2011 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Mar 14 13:23:29 2011 Subject: [Histonet] VALIDATION of a new clone In-Reply-To: <24A4826E8EF0964D86BC5317306F58A5556A2EC5C6@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A5556A2EC5C6@mmc-mail.ad.mhsil.com> Message-ID: Hi Jim, If it's a new clone then a new valiation should be performed. I know of many instances where one clone stains something that another doesn't. If this is regarding Ventana not selling certains clones, those clones are still available from Cell Marque directly. Mark On Mon, Mar 14, 2011 at 11:16 AM, Vickroy, Jim wrote: > I am wondering what others are doing when they validate a new clone of a > antibody that you have used in the lab for quite sometime. When we > introduce a new antibody obviously we go through a pretty extensive > validation process including sampling of around 20 - 30 specimens, some > presumed positive cases and some presumed negative cases. We also try to > include in the positive cases some that are strongly positive and others > that are weakly positive. > > Lately several of our regular antibodies are now no longer available in the > same clone. I am interested in how different labs would validate the new > clone to be used in the lab. It would seem to me that the validation > process would not have to be as extensive since we have already established > that the antibody works well in our laboratory. I know if it was the same > clone but a different lot we would just do a comparison of the old lot with > the new lot, but what about a different clone? I realize that the methods > are going to be diverse but I would appreciate hearing from others. > > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DKBoyd <@t> chs.net Mon Mar 14 13:31:43 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Mon Mar 14 13:31:52 2011 Subject: [Histonet] Adequacy charges Message-ID: Can we charge adequacy charges for each pass on an FNA. We enter each specimen separately and make cytospins on the rest of the material, and generate a report for each. We charge each case an 88108 for the cytospins. But are we allowed to charge an adequacy charge on each case. Also can the pathologist charge for each adequacy pass. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From jaylundgren <@t> gmail.com Mon Mar 14 15:03:16 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Mar 14 15:03:22 2011 Subject: [Histonet] g ratio In-Reply-To: <1FB9362B907EEC43959F72445AC63B637CFEA3@apollon.cit_serveur.com> References: <1FB9362B907EEC43959F72445AC63B637CFEA3@apollon.cit_serveur.com> Message-ID: Bonjour, I think a Luxol Fast Blue is the standard for visualizing the myelin sheath. Here's a free tip; leave your slides in the LFB stain overnight, instead of the shorter time specified in most protocols. You're going to decolorize them anyway. Decolorize the slides until you don't see the stain run off anymore. You get a really brilliant LFB this way. The Pathologists I've worked with love it. Comment dit on "myelin sheath" en Francaise? Vive l'esprit de Lafayette! Jay A. Lundgren M.S., HTL(ASCP) From Sandra.Harrison3 <@t> va.gov Mon Mar 14 15:40:57 2011 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Mon Mar 14 15:41:05 2011 Subject: [Histonet] Control Slides In-Reply-To: <48441ED607EC40B6AF54090AB8300EA1@HP2010> References: <1D9B439FC446244095353DCA4FC23DD204F445CBBA@MWNMAIL00.ameripath.local> <48441ED607EC40B6AF54090AB8300EA1@HP2010> Message-ID: We dry the control slide at the same time that we dry the tissue being stained, since controls are supposed to be handled in the same manner as the test tissue. That being said, I guess if we were truly following that rule, we would cut the control on the same day we cut the test sample. :-) "Other people say that, after they dry the slide, they dip the slide in paraffin, to cover the tissue, so that the air doesn't touch the tissue during the months before the slide is used in a stain." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Thursday, March 10, 2011 6:39 PM To: Amador, Amanda; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Control Slides I've noticed that our spirochete control slides don't stain as intense starting about 3 months after they are sectioned, and that they stop staining by about 6 months. (This is with a silver stain.) I've talked with other histotechs, and they say they've seen the same phenomenon with AFB and Gram controls. (We use ours up too quickly - we never have 6-12 months old control slides for these, so I can't attest to this. ) Bancroft's book talks about this phenomenon with amyloid, and suggests that oxidation of the tissue (proteins) due to exposure to air may be the cause. I'm guessing the it probably applies to the precut microorganism control slides, too. We only cut enough slides for 6 months, and date them. Other people say they put their cut control slides in a slide box with a lid after drying, and then place the box in the refrig, as cold slows down the chemical change, and the lid keeps the moving air off the slide. Other people say that, after they dry the slide, they dip the slide in paraffin, to cover the tissue, so that the air doesn't touch the tissue during the months before the slide is used in a stain. Just 3 suggestions to stop this problem. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -------------------------------------------------- From: "Amador, Amanda" Sent: Wednesday, March 09, 2011 10:22 PM To: Subject: [Histonet] Control Slides > Is there guidelines for how long special stains controls are good for once > they are cut? We have spirochetes for our Steiner that is from 2007 and > we are having issues. > > Amanda Amador, HT(ASCP)CM > AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | > Indianapolis, IN 46219 | phone 317.275.8052 | > aamador@ameripath.com | > www.AmeriPath.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From med_leni <@t> yahoo.com Mon Mar 14 15:57:48 2011 From: med_leni <@t> yahoo.com (Pop Elena) Date: Mon Mar 14 15:57:51 2011 Subject: [Histonet] immunostaining question Message-ID: <694616.8213.qm@web30903.mail.mud.yahoo.com> Hello everybody, I am trying some new antibodies on parafin embeded radical prostactectomies tissue sections and I started with the highest recomended dilution but it seems I did not get any staining. I already counterstained the slides w/ hematoxylin and I was wondering if it's possible to go a few steps back and incubate again with a higher concentration of the antibody? I have a limited amount of tissue sections for "testing". Any imput is appreciated! Thanks, Leni From rjbuesa <@t> yahoo.com Mon Mar 14 16:01:53 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 14 16:01:56 2011 Subject: [Histonet] immunostaining question In-Reply-To: <694616.8213.qm@web30903.mail.mud.yahoo.com> Message-ID: <704175.76355.qm@web65714.mail.ac4.yahoo.com> Yes you can, especially if the antigen is not nuclear. Ren?? J. ? --- On Mon, 3/14/11, Pop Elena wrote: From: Pop Elena Subject: [Histonet] immunostaining question To: histonet@lists.utsouthwestern.edu Date: Monday, March 14, 2011, 4:57 PM Hello everybody, I am trying some new antibodies on parafin embeded radical prostactectomies tissue sections and I started with the highest recomended dilution but it seems I did not get any staining. I already counterstained the slides w/ hematoxylin and I was wondering if it's possible to go a few steps back and incubate again with a higher concentration of the antibody? I have a limited amount of tissue sections for "testing". Any imput is appreciated! Thanks, Leni ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Mon Mar 14 19:03:47 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Mar 14 19:03:51 2011 Subject: [Histonet] Research and Clinical Labs Message-ID: Hi, Let me preface this by stating that I don't think this is a good idea and it is not what we are doing here. I have a hypothetical question though. What would be the regulatory ramifications of merging a research lab testing animal specimens (not regulated by any agencies other than Best Practices and OSHA) with a traditional (CAP, JACHO) clinical histology lab. What equipment, if any, could be shared? In the event of a worst case scenario (like an inspector noticing mouse tissue and human tissue on the same processor at the same time or expired chemicals not labeled "research use only") which regulations would be violated and how would one find out potential fines that could be incurred. I have heard that there are labs that are doing this. What precautions should a lab take if they end up venturing down this path. Thanks, Amos From Michel.Bataillon <@t> citox.com Tue Mar 15 02:44:29 2011 From: Michel.Bataillon <@t> citox.com (Bataillon Michel) Date: Tue Mar 15 02:44:42 2011 Subject: [Histonet] g ratio References: <1FB9362B907EEC43959F72445AC63B637CFEA3@apollon.cit_serveur.com> Message-ID: <1FB9362B907EEC43959F72445AC63B637CFEAE@apollon.cit_serveur.com> Bonjour, Thanks a lot. "Myelin sheath" se dit "gaine de my?line" en fran?ais. Michel BATAILLON Histology Evreux, France ________________________________ De : Jay Lundgren [mailto:jaylundgren@gmail.com] Envoy? : lundi 14 mars 2011 21:03 ? : Bataillon Michel Cc : histonet@lists.utsouthwestern.edu Objet : Re: [Histonet] g ratio Bonjour, I think a Luxol Fast Blue is the standard for visualizing the myelin sheath. Here's a free tip; leave your slides in the LFB stain overnight, instead of the shorter time specified in most protocols. You're going to decolorize them anyway. Decolorize the slides until you don't see the stain run off anymore. You get a really brilliant LFB this way. The Pathologists I've worked with love it. Comment dit on "myelin sheath" en Francaise? Vive l'esprit de Lafayette! Jay A. Lundgren M.S., HTL(ASCP) ************************************************************* Ce message et toutes les pi?ces jointes sont confidentiels et ?tablis ? l'attention exclusive de ses destinataires. Si vous recevez ce message par erreur, merci de le d?truire et d'en avertir imm?diatement l'exp?diteur. Toute utilisation de ce message non conforme ? sa destination, toute diffusion ou toute publication, totale ou partielle, est interdite. Internet ne permettant pas de garantir l'int?grit? de ce message, le CIT d?cline toute responsabilit? au titre de ce message s'il a ?t? alt?r?, d?form? ou falsifi?. Prot?geons ensemble l'environnement, n'imprimons ce mail (et ses pi?ces jointes) que si cela est n?cessaire. This transmission and any attachments are confidential and intended solely for the use of the addressee(s). If you are not an intended recipient, please notify us immediately by replying to the message and deleting it from your computer. In this case, any unauthorized use, copying or distribution is strictly prohibited. Please be aware that E-mails are susceptible to alteration and their integrity cannot be guaranteed. CIT shall not be liable for this E-mail if corrupted, changed or falsified. Please consider the environment before printing this E-mail. ************************************************************* From BMolinari <@t> heart.thi.tmc.edu Tue Mar 15 05:57:47 2011 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Tue Mar 15 05:58:42 2011 Subject: [Histonet] Bielschowskys stain Message-ID: Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) From BMolinari <@t> heart.thi.tmc.edu Tue Mar 15 06:00:20 2011 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Tue Mar 15 06:01:13 2011 Subject: [Histonet] Bielschowskys Stain Message-ID: Hi , An investigator would like show axonal degeneration in peripheral nerves. Is Bielschowsky's the preferred stain? Thanks! Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) From catherinesimonson <@t> gmail.com Tue Mar 15 06:40:46 2011 From: catherinesimonson <@t> gmail.com (Catherine Simonson) Date: Tue Mar 15 06:40:49 2011 Subject: [Histonet] equine bone decal In-Reply-To: <4010584DE26D90419367C0A5F620E9633027F1C424@MBXB.exchange.cornell.edu> References: <4010584DE26D90419367C0A5F620E9633027F1C424@MBXB.exchange.cornell.edu> Message-ID: sodium citrate buffered formic acid. works well and much faster than edta. Catherine On Fri, Mar 11, 2011 at 3:19 PM, Mary Lou Norman wrote: > Hi, > We've been using EDTA to decal and of course it takes forever. I would love > to hear what others are using for large bones, joints, etc. We do not work > on rats or mice, just horses. > > Thanks. Many prayers to Japan and beyond. > Mary Lou > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From idimenstein <@t> hotmail.com Tue Mar 15 07:30:45 2011 From: idimenstein <@t> hotmail.com (Izak Dimenstein) Date: Tue Mar 15 07:30:51 2011 Subject: [Histonet] equine bone decal In-Reply-To: References: , <4010584DE26D90419367C0A5F620E9633027F1C424@MBXB.exchange.cornell.edu>, Message-ID: Dear Mary Lou Norman: My experience is only in surgical pathology. The main point is in a good section before decalcification. In this situation the latter does not matter substantially, except bone marrows or tiny bone biopsies. www.grossing-technology.com or Dimenstein IB. Bone grossing techniques: helpful hints and procedures. Annals of Diagnostic Pathology 2008; 12:191-198. Best wishes, Izak Dimenstein > Date: Tue, 15 Mar 2011 07:40:46 -0400 > From: catherinesimonson@gmail.com > To: mlm11@cornell.edu > Subject: Re: [Histonet] equine bone decal > CC: histonet@lists.utsouthwestern.edu > > sodium citrate buffered formic acid. works well and much faster than edta. > > Catherine > > On Fri, Mar 11, 2011 at 3:19 PM, Mary Lou Norman wrote: > > > Hi, > > We've been using EDTA to decal and of course it takes forever. I would love > > to hear what others are using for large bones, joints, etc. We do not work > > on rats or mice, just horses. > > > > Thanks. Many prayers to Japan and beyond. > > Mary Lou > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Mar 15 07:35:51 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Mar 15 07:36:51 2011 Subject: [Histonet] immunostaining question In-Reply-To: <694616.8213.qm@web30903.mail.mud.yahoo.com> References: <694616.8213.qm@web30903.mail.mud.yahoo.com> Message-ID: <8C023B4AB999614BA4791BAEB26E273801002997@UWHC-MAIL01.uwhis.hosp.wisc.edu> Yes, most definitely. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pop Elena Sent: Monday, March 14, 2011 3:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] immunostaining question Hello everybody, I am trying some new antibodies on parafin embeded radical prostactectomies tissue sections and I started with the highest recomended dilution but it seems I did not get any staining. I already counterstained the slides w/ hematoxylin and I was wondering if it's possible to go a few steps back and incubate again with a higher concentration of the antibody? I have a limited amount of tissue sections for "testing". Any imput is appreciated! Thanks, Leni _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Mar 15 07:55:54 2011 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Tue Mar 15 07:55:59 2011 Subject: [Histonet] Control Slides Message-ID: <20110315055554.f86bd30e73b823f57b516b5451216a98.cdb89e8265.wbe@email00.secureserver.net> I do not melt dry my cut control slides until i need to use them, i do store them in boxes at 4c, i have recently run into this problem them in th Patsy -------- Original Message -------- Subject: RE: From: "Harrison, Sandra C." <[1]Sandra.Harrison3@va.gov> Date: Mon, To: "Lee & Peggy Wenk" <[2]lpwenk@sbcglobal.net>, "Amador, Amanda" &l uthwestern.edu> We dry the control slide at the same time tha being stained, since controls are supposed to be han manner as the test tissue. That being said, I guess if that rule, we would cut the control on the same sample. :-) "Other people say that, after they dry the slide, they dip the slide in paraffin, to cover the tissu so that the air doesn't touch the tissue during the months before slide is used in a stain." -----Original Message----- From: [5]histone [[6]mailto:histonet-bounces@lists.utsouthwestern.e Lee & Peggy Wenk Sent: Thursday, March 10, 2 To: Amador, Amanda; [7]histonet@lists.utsouthwestern.edu Subject: Re: [Histone I've noticed that our spirochete control slides do intense starting about 3 months after they are sectioned, staining by about 6 months. (This is with a silver s I've talked with other histotechs, and they say they've seen same phenomenon with AFB and Gram controls. (We use ours up too qui we never have 6-12 months old control slides for these, so I can attest to this. ) Bancroft's book talks about this phenome suggests that oxidation of the tissue (protein cause. I'm guessing the it prob control slides, too. We only cut enough slides for 6 months, and date them. Other people say they put their cut control slides in a slide box with a lid after dryi and then place the box in the refrig, as cold slows down the che change, and the lid keeps the moving air off the slide. Other that, after they dry the slide, they dip the slide in par tissue, so that the air doesn't touch the tissue slide is used in a stain. Just 3 sugge Peggy A. Wenk, HTL(ASCP)SLS Beaumont Royal Oak, MI 48073 ----------------------------------- From: "Amador, Amanda" <[8]aamador@ameripath.com> Sent: Wednesday, March 09, 201 To: <[9] Subject: [Histonet] Control Sl > Is there guidelines for how long special stains controls a for once > they are cut? We have spirochetes for our Stei 2007 and > we are having issues. > > > AmeriPath | Histology Group Lead/Trainer Suite A | > Indianapolis, IN 46219 | phon > [10]aamador@ > [12]www.AmeriPath.com > _______________________________________________ > Histonet mailing list > [13]Histonet@lists.utsouthwestern.edu > [14]http://lists.utso __________________ Histonet mailing list [15]Histonet@lists.utsouthwestern.edu[16]htt p://lists.utsouthwestern.edu/mailman/listinfo/histonet _________ Histonet mailing list [17]Histonet@lists.utsouthwestern [18]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. file://localhost/tmp/3D"ma 2. file://localhost/tmp/3D"mailto 3. 3D"mailto:aamador@ameripath.com" 4. 3D"mailto:histonet@lists.utsouthwestern.edu" 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 6. 3D"mailto:histonet-bounc 7. 3D"mailto:histonet@lists.utsouth 8. 3D"mailto:aamador@ame 9. 3D"mailto:histonet@lists.utsouthwestern.edu" 10. 3D"mailto:aamador@ameripath.com" 11. 3D"http://aamador@ameripath.com>"/ 12. 3D"http://www.AmeriPath.com&l=/ 13. 3D"mailto:Histonet@lists.uts 14. file://localhost/tmp/3D"h 15. file://localhost/tmp/3D"mailto 16. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 17. ="mailto:Histonet@lists.utsouthwestern.edu" 18. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/his From hfedor <@t> jhmi.edu Tue Mar 15 08:31:37 2011 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Tue Mar 15 08:32:08 2011 Subject: [Histonet] Control Slides In-Reply-To: <20110315055554.f86bd30e73b823f57b516b5451216a98.cdb89e8265.wbe@email00.secureserver.net> References: <20110315055554.f86bd30e73b823f57b516b5451216a98.cdb89e8265.wbe@email00.secureserver.net> Message-ID: Hello, We have been storing our slides in small zip loc bags at -20 and have data suggesting that slides stored in this fashion are reasonably well preserved after 3- 5 years. This was done with TMA slides. Was not done for special stain slides but for IHC. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pruegg@ihctech.net Sent: Tuesday, March 15, 2011 8:56 AM To: Harrison,Sandra C. Cc: histonet@lists.utsouthwestern.edu; Amador, Amanda Subject: RE: [Histonet] Control Slides I do not melt dry my cut control slides until i need to use them,=d i do store them in boxes at 4c, i have recently run into this problem =th blocks, so now i seal all my blocks with paraffin and store them in th?ridge as well. Patsy -------- Original Message -------- Subject: RE:=istonet] Control Slides From: "Harrison, Sandra C." <[1]Sandra.Harrison3@va.gov> Date: Mon,=rch 14, 2011 1:40 pm To: "Lee & Peggy Wenk" <[2]lpwenk@sbcglobal.net>, "Amador, Amanda" &l=[3]aamador@ameripath.com>, &=;[4]histonet@lists.utso uthwestern.edu> We dry the control slide at the same time tha=e dry the tissue being stained, since controls are supposed to be han=ed in the same manner as the test tissue. That being said, I guess if = were truly following that rule, we would cut the control on the same ?y we cut the test sample. :-) "Other people say that, after they dry the slide, they dip the slide in paraffin, to cover the tissu= so that the air doesn't touch the tissue during the months before=e slide is used in a stain." -----Original Message----- From: [5]histone=bounces@lists.utsouthwestern.edu [[6]mailto:histonet-bounces@lists.utsouthwestern.e=] On Behalf Of Lee & Peggy Wenk Sent: Thursday, March 10, 21 6:39 PM To: Amador, Amanda; [7]histonet@lists.utsouthwestern.edu Subject: Re: [Histone= Control Slides I've noticed that our spirochete control slides do=t stain as intense starting about 3 months after they are sectioned, =d that they stop staining by about 6 months. (This is with a silver s=in.) I've talked with other histotechs, and they say they've seen =e same phenomenon with AFB and Gram controls. (We use ours up too qui=ly - we never have 6-12 months old control slides for these, so I can= attest to this. ) Bancroft's book talks about this phenome=n with amyloid, and suggests that oxidation of the tissue (protein= due to exposure to air may be the cause. I'm guessing the it prob?ly applies to the precut microorganism control slides, too. We only cut enough slides for 6 months, and date them. Other people say they put their cut control slides in a slide box with a lid after dryi=, and then place the box in the refrig, as cold slows down the che=cal change, and the lid keeps the moving air off the slide. Other =ople say that, after they dry the slide, they dip the slide in par?fin, to cover the tissue, so that the air doesn't touch the tissue=ring the months before the slide is used in a stain. Just 3 sugge=ions to stop this problem. Peggy A. Wenk, HTL(ASCP)SLS Beaumont=spital Royal Oak, MI 48073 -----------------------------------=------------- From: "Amador, Amanda" <[8]aamador@ameripath.com> Sent: Wednesday, March 09, 2010:22 PM To: <[9]=stonet@lists.utsouthwestern.edu> Subject: [Histonet] Control Sl=es > Is there guidelines for how long special stains controls a= good for once > they are cut? We have spirochetes for our Stei=r that is from 2007 and > we are having issues. > >=anda Amador, HT(ASCP)CM > AmeriPath | Histology Group Lead/Trainer=560 N Shadeland Ave, Suite A | > Indianapolis, IN 46219 | phon?17.275.8052 | > [10]aamador@=eripath.com; | > [12]www.AmeriPath.com; > > _______________________________________________ > Histonet mailing list > [13]Histonet@lists.utsouthwestern.edu > [14]http://lists.utso=hwestern.edu/mailman/listinfo/histonet __________________=___________________________ Histonet mailing list [15]Histonet@lists.utsouthwestern.edu[16]htt p://lists.utsouthwestern.edu/mailman/listinfo/histonet _________=____________________________________ Histonet mailing list [17]Histonet@lists.utsouthwestern=du [18]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. file://localhost/tmp/3D"ma 2. file://localhost/tmp/3D"mailto 3. 3D"mailto:aamador@ameripath.com" 4. 3D"mailto:histonet@lists.utsouthwestern.edu" 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 6. 3D"mailto:histonet-bounc 7. 3D"mailto:histonet@lists.utsouth 8. 3D"mailto:aamador@ame 9. 3D"mailto:histonet@lists.utsouthwestern.edu" 10. 3D"mailto:aamador@ameripath.com" 11. 3D"http://aamador@ameripath.com>"/ 12. 3D"http://www.AmeriPath.com&l= 13. 3D"mailto:Histonet@lists.uts 14. file://localhost/tmp/3D"h 15. file://localhost/tmp/3D"mailto 16. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 17. ="mailto:Histonet@lists.utsouthwestern.edu" 18. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/his_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Tue Mar 15 09:16:55 2011 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Tue Mar 15 09:17:01 2011 Subject: [Histonet] PAS for basement membrane Message-ID: <379A927A452F3D43A3C8705F4E67905F16661F85B5@EX05.net.ucsf.edu> Hi all, I'm having a problem with my PAS stain. My pathologist says that glycogen and fungus stain beautifully but basement membranes are pale. We are using 0.5% periodic acid for 10 minutes and schiff's for 25 minutes followed by 5 min rinse in warm/hot water. Any thoughts? Thank you in advance! Thanks Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 From jqb7 <@t> cdc.gov Tue Mar 15 09:20:46 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Mar 15 09:21:14 2011 Subject: [Histonet] RE: PAS for basement membrane In-Reply-To: <379A927A452F3D43A3C8705F4E67905F16661F85B5@EX05.net.ucsf.edu> References: <379A927A452F3D43A3C8705F4E67905F16661F85B5@EX05.net.ucsf.edu> Message-ID: We wash after Schiff's for 10 minutes in regular temp tap water. Don't know if that helps or not...... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Tuesday, March 15, 2011 10:17 AM To: histonet Subject: [Histonet] PAS for basement membrane Hi all, I'm having a problem with my PAS stain. My pathologist says that glycogen and fungus stain beautifully but basement membranes are pale. We are using 0.5% periodic acid for 10 minutes and schiff's for 25 minutes followed by 5 min rinse in warm/hot water. Any thoughts? Thank you in advance! Thanks Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jeberry <@t> umich.edu Tue Mar 15 10:15:30 2011 From: jeberry <@t> umich.edu (Jan Berry) Date: Tue Mar 15 10:15:37 2011 Subject: [Histonet] bone decal In-Reply-To: <201103131702.p2DH2dtX012572@brazil.mail.umich.edu> References: <201103131702.p2DH2dtX012572@brazil.mail.umich.edu> Message-ID: Cal-Ex II (available from Fisher and possibly elsewhere) fixes and decals together. Depending on the downstream tissue applications, it can be good, and is MUCH faster than EDTA. Ditto on the thoughts and hopes for Japan's recovery. Jan Berry University of Michigan From JMacDonald <@t> mtsac.edu Tue Mar 15 10:22:15 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Mar 15 10:22:22 2011 Subject: [Histonet] PAS for basement membrane In-Reply-To: <379A927A452F3D43A3C8705F4E67905F16661F85B5@EX05.net.ucsf.edu> Message-ID: Try decreasing the time in periodic acid. Basement membrane will over oxidize faster than the fungus. Some docs like the over-oxidation to high-light the fungus. "Martin, Erin" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/15/2011 07:19 AM To histonet cc Subject [Histonet] PAS for basement membrane Hi all, I'm having a problem with my PAS stain. My pathologist says that glycogen and fungus stain beautifully but basement membranes are pale. We are using 0.5% periodic acid for 10 minutes and schiff's for 25 minutes followed by 5 min rinse in warm/hot water. Any thoughts? Thank you in advance! Thanks Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruppert.amysue <@t> marshfieldclinic.org Tue Mar 15 10:41:11 2011 From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue) Date: Tue Mar 15 10:41:26 2011 Subject: [Histonet] PAS for basement membrane&In-Reply-To= Message-ID: <201103151541.p2FFfDDv009952@spamfilt> We have found out that we need to use freshly prepared 0.5% Periodic Acid. Your times seem appropriate. amysue ruppert marshfield labs, Histology. ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From mcauliff <@t> umdnj.edu Tue Mar 15 11:00:26 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Mar 15 10:57:11 2011 Subject: [Histonet] PAS for basement membrane In-Reply-To: <379A927A452F3D43A3C8705F4E67905F16661F85B5@EX05.net.ucsf.edu> References: <379A927A452F3D43A3C8705F4E67905F16661F85B5@EX05.net.ucsf.edu> Message-ID: <4D7F8D1A.80303@umdnj.edu> Make the periodic acid fresh that day. Use 3 2 minute washes in 0.5% sodium meta-bisulfite instead of water. Geoff On 3/15/2011 10:16 AM, Martin, Erin wrote: > Hi all, > > I'm having a problem with my PAS stain. My pathologist says that glycogen and fungus stain beautifully but basement membranes are pale. We are using 0.5% periodic acid for 10 minutes and schiff's for 25 minutes followed by 5 min rinse in warm/hot water. Any thoughts? Thank you in advance! > > Thanks > Erin > > Erin Martin, Histology Supervisor > UCSF Department of Dermatopathology > 415-353-7248 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From kjohnson <@t> moellerdermatology.com Tue Mar 15 11:31:44 2011 From: kjohnson <@t> moellerdermatology.com (kristina johnson) Date: Tue Mar 15 11:31:50 2011 Subject: [Histonet] validation Message-ID: <000901cbe32e$79d40550$6d7c0ff0$@com> I work for a dermatologist that is starting up a brand new Histology lab and I have never had to validate machines before (everywhere I worked already had already had that completed). Is there a set amount of runs on the processor that have to be proformed? How many slides need to be viewed in order to validate the stainer? What type of documentation is needed? The list goes on.where would I find this information? The Doctor is not interested in CAP so we will not be inspected by them but does CLIA have any involvement in validation.? From jaylundgren <@t> gmail.com Tue Mar 15 12:05:56 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Mar 15 12:06:01 2011 Subject: [Histonet] Research and Clinical Labs In-Reply-To: References: Message-ID: Amos, I don't know the answer, but this is a really interesting question. I have worked both in the Research and Clinical Histology Laboratory and I have never seen the two mixed, which begs the question, "Why?". Maybe it's just because, if you go through the trouble and expense of starting a CAP accredited lab, processing patient specimens is so remunerative for the owner of the Pathology lab, why would they want to do anything else? Conversely, if you are a university or corporate owner of a Research Laboratory, your PI would get PO'ed if some Pathology Resident adding blocks to the tissue processor after hours, and not restarting it, messes us an experiment that had taken a year and ten thousand man-hours of meticulous lab time to get to the tissue block stage. Maybe there is a pertinent regulation. In my opinion, it sounds like a really good way to spread zoonotic infection. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) From ruppert.amysue <@t> marshfieldclinic.org Tue Mar 15 12:09:01 2011 From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue) Date: Tue Mar 15 12:09:06 2011 Subject: [Histonet] PAS for basement membrane&In-Reply-To= Message-ID: <201103151708.p2FH8rD0019754@spamfilt> Yes, it is good to use the metabisulfite rinses to remove any excess schiff reagent after the schiff step. But after the metabisulfite rinses, you still need to go into TAP water for several minutes to have the full color of the PAS reaction develop. It will get much brighter after the final tap water rinse. amysue ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From jaylundgren <@t> gmail.com Tue Mar 15 12:12:02 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Mar 15 12:12:07 2011 Subject: [Histonet] PAS for basement membrane In-Reply-To: <4D7F8D1A.80303@umdnj.edu> References: <379A927A452F3D43A3C8705F4E67905F16661F85B5@EX05.net.ucsf.edu> <4D7F8D1A.80303@umdnj.edu> Message-ID: Geoff, A PAS is not really the best stain for basement membranes. I think your Pathologist wants to look at a PAMS. You can make the basement membrane look like you drew it on the slide with a Sharpie marker. After the silver you can counterstain however you want. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) From jaylundgren <@t> gmail.com Tue Mar 15 12:14:10 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Mar 15 12:15:24 2011 Subject: [Histonet] PAS for basement membrane In-Reply-To: References: <379A927A452F3D43A3C8705F4E67905F16661F85B5@EX05.net.ucsf.edu> <4D7F8D1A.80303@umdnj.edu> Message-ID: Sorry, I should have addressed this to Erin. On Tue, Mar 15, 2011 at 12:12 PM, Jay Lundgren wrote: > Geoff, > From lguidot <@t> ameripath.com Tue Mar 15 12:42:59 2011 From: lguidot <@t> ameripath.com (Guidot, Lucie) Date: Tue Mar 15 12:45:04 2011 Subject: [Histonet] Please add Message-ID: <301E08EC3961144EA578DEFE6EBFA7E60539A8C491@MWNMAIL00.ameripath.local> Llaude@ameripath.com From liz <@t> premierlab.com Tue Mar 15 12:45:19 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Mar 15 12:45:23 2011 Subject: [Histonet] Research and Clinical Labs In-Reply-To: Message-ID: Amos We are a GLP compliant contract research lab, and we are currently in the process of applying for a CLIA accreditation. I don't see why you would need to have separate equipment, etc. In our case we will not be processing standard diagnostic samples but we feel that the CLIA accreditation will be good for us to have in order to process clinical trial study samples. Maybe Haji or someone from Phenopath labs can comment I believe both of those labs are both CLIA accredited and GLP compliant. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Monday, March 14, 2011 6:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Research and Clinical Labs Hi, Let me preface this by stating that I don't think this is a good idea and it is not what we are doing here. I have a hypothetical question though. What would be the regulatory ramifications of merging a research lab testing animal specimens (not regulated by any agencies other than Best Practices and OSHA) with a traditional (CAP, JACHO) clinical histology lab. What equipment, if any, could be shared? In the event of a worst case scenario (like an inspector noticing mouse tissue and human tissue on the same processor at the same time or expired chemicals not labeled "research use only") which regulations would be violated and how would one find out potential fines that could be incurred. I have heard that there are labs that are doing this. What precautions should a lab take if they end up venturing down this path. Thanks, Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Tue Mar 15 12:53:15 2011 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Tue Mar 15 12:53:23 2011 Subject: [Histonet] Please add In-Reply-To: <301E08EC3961144EA578DEFE6EBFA7E60539A8C491@MWNMAIL00.ameripath.local> References: <301E08EC3961144EA578DEFE6EBFA7E60539A8C491@MWNMAIL00.ameripath.local> Message-ID: <4D7FA78B.9020400@vneubert.com> Almost looks like Friday :-D Dear Lucie, please go to http://lists.utsouthwestern.edu/mailman/listinfo/histonet and fill out the form at the bottom of the page to subscribe. Regards From Candy.A.Bales <@t> uth.tmc.edu Tue Mar 15 13:57:37 2011 From: Candy.A.Bales <@t> uth.tmc.edu (Bales, Candy A) Date: Tue Mar 15 13:57:41 2011 Subject: [Histonet] recyclers Message-ID: <62C915811DD5A142851D95CA6BC5D1E41960C761A4@UTHCMS1.uthouston.edu> A friend works in a hospital lab and they are pressuring her to buy recyclers for formalin, alcohol, xylene and xylene substitutes. She is asking about pros and cons and whether or not it is worth the effort, time and cost of the units. Any information is appreciated. Thank you Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax From rjbuesa <@t> yahoo.com Tue Mar 15 14:38:01 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 15 14:38:05 2011 Subject: [Histonet] recyclers In-Reply-To: <62C915811DD5A142851D95CA6BC5D1E41960C761A4@UTHCMS1.uthouston.edu> Message-ID: <359648.60034.qm@web65709.mail.ac4.yahoo.com> A good recycling system (like the one I used from B/R Corp.) will be able to recycle alcohol, xylene and its substitutes, but I would never recycle formalin (unnecessary additional exposure, no matter which system you use). Ren? J. ? ? --- On Tue, 3/15/11, Bales, Candy A wrote: From: Bales, Candy A Subject: [Histonet] recyclers To: "histonet@lists.utsouthwestern.edu" Date: Tuesday, March 15, 2011, 2:57 PM A friend works in a hospital lab and they are pressuring her to buy recyclers for formalin, alcohol, xylene and xylene substitutes. She is asking about pros and cons and whether or not it is worth the effort, time and cost of the units. Any information is appreciated. Thank you Candy Candy Bales, HT Chief Histologist The University? of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSiena <@t> statlab.com Tue Mar 15 15:21:14 2011 From: DSiena <@t> statlab.com (Debra Siena) Date: Tue Mar 15 15:21:20 2011 Subject: [Histonet] Diff Quik or Hema Diff on Frozen Sections Message-ID: Hi All, If anyone is using the Diff Quik or Hema Diff stains on frozen sections, would you mind sharing your protocol, especially in regards to how the FS slides are handled after cutting, are they air dried, or not and what fixative if any do you use before staining. Thanks in advance, I appreciate all the helpful information that I receive on Histonet. Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsiena@statlab.com | www.statlab.com From alyssa <@t> alliedsearchpartners.com Tue Mar 15 15:25:44 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Mar 15 15:25:50 2011 Subject: [Histonet] Open Jobs Message-ID: Hi Histonet! Just a quick note to let you know we have some new full time and part time job openings for permanent/direct hire in the following states. Contact me to see if we have a position in your area! Thank you! NY, GA, LA, CA, NC, WI, FL, and NJ Send us a quick reply to inquire about any of the above states. We have Staff/Bench level all the way up to Management level positions. I look forward to hearing from you! -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with ?Remove.? * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From Candy.A.Bales <@t> uth.tmc.edu Tue Mar 15 15:48:30 2011 From: Candy.A.Bales <@t> uth.tmc.edu (Bales, Candy A) Date: Tue Mar 15 15:48:35 2011 Subject: [Histonet] Thank you RE: recyclers Message-ID: <62C915811DD5A142851D95CA6BC5D1E41960C761E5@UTHCMS1.uthouston.edu> Thank you all for the information you have sent regarding the recyclers. I have forwarded them to my friend and she will be well informed when they do purchase a recycler. Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax From tjasper <@t> copc.net Tue Mar 15 16:18:56 2011 From: tjasper <@t> copc.net (Thomas Jasper) Date: Tue Mar 15 16:19:02 2011 Subject: [Histonet] California Histology Society Message-ID: <90354A475B420441B2A0396E5008D49692C00C@copc-sbs.COPC.local> Hello Histofolks, Am wondering if someone from the California Society would be kind enough to contact me regarding the state convention this year? I'd like to send one of my staff. I was under the impression that it commences sometime in May. I've checked the website and haven't found any information on this. My contact information is below. Thank you kindly, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net From Nacaela.Johnson <@t> USONCOLOGY.COM Tue Mar 15 16:21:31 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Tue Mar 15 16:21:34 2011 Subject: [Histonet] jobs Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D5AF@txhous1eb012.uson.usoncology.int> I know a certified tech that may be looking for a job in the Kansas City area. Does anyone have any openings? Thanks, Nacaela Johnson, HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From JMacDonald <@t> mtsac.edu Tue Mar 15 17:51:14 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Mar 15 17:51:24 2011 Subject: [Histonet] California Histology Society In-Reply-To: <90354A475B420441B2A0396E5008D49692C00C@copc-sbs.COPC.local> Message-ID: The California Society Symposium is scheduled for May 13-15 in Concord CA. The program is about to go to print and will be on the website shortly. "Thomas Jasper" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/15/2011 02:21 PM To cc Subject [Histonet] California Histology Society Hello Histofolks, Am wondering if someone from the California Society would be kind enough to contact me regarding the state convention this year? I'd like to send one of my staff. I was under the impression that it commences sometime in May. I've checked the website and haven't found any information on this. My contact information is below. Thank you kindly, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Tue Mar 15 19:53:45 2011 From: shive003 <@t> umn.edu (shive003@umn.edu) Date: Tue Mar 15 19:53:50 2011 Subject: [Histonet] Research and Clinical Labs In-Reply-To: References: Message-ID: It's my understanding that infectious organisms are rendered inactive when fixed in an aldehyde-based fixative (except for abnormal prion proteins). So, the only concern that one would need to address is if frozens are desired. Due safety precautions must be taken, of course, in the initial retrieval/excision of tissue from animals in the necropsy suite when zoonotic infections are suspected. I don't see a problem with using the same equipment. Processing schedules (times) may have to be adjusted for animal tissue, however, so dedicated processing runs may be necessary for them. Jan Shivers U of MN Veterinary Diagnostic Lab On Mar 15 2011, Jay Lundgren wrote: >Amos, > > I don't know the answer, but this is a really interesting question. I >have worked both in the Research and Clinical Histology Laboratory and I >have never seen the two mixed, which begs the question, "Why?". > Maybe it's just because, if you go through the trouble and expense of >starting a CAP accredited lab, processing patient specimens is so >remunerative for the owner of the Pathology lab, why would they want to do >anything else? > Conversely, if you are a university or corporate owner of a Research Laboratory, your PI would get PO'ed if some Pathology Resident adding blocks >to the tissue processor after hours, and not restarting it, messes us an >experiment that had taken a year and ten thousand man-hours of meticulous >lab time to get to the tissue block stage. Maybe there is a pertinent regulation. In my opinion, it sounds like a >really good way to spread zoonotic infection. > > >Sincerely, > >Jay A. Lundgren M.S., HTL (ASCP) >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From velma.jordan <@t> gwu-hospital.com Tue Mar 15 20:19:30 2011 From: velma.jordan <@t> gwu-hospital.com (Jordan, Velma) Date: Tue Mar 15 20:19:38 2011 Subject: [Histonet] Light hematoxylin staining in H&E Message-ID: <564683BEC104BA44851D9A58F5BB70E469B965135C@CORP-EXVS03.corp.uhsinc.biz> We have been troubleshooting complaints of hematoxylin staining light in our H&E. The main complaint is no differentiation between the nucleus and cytoplasm. We are using Richard-Allan hematoxylin ,eosin,clarifier and bluing. We tried surgipath, changing times, pH the H2O and any other thing we could think of, but the problem remains. Does anyone have a suggestion? Velma Jordan UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. From sdeppeler <@t> ksu.edu.sa Wed Mar 16 03:30:59 2011 From: sdeppeler <@t> ksu.edu.sa (Stacy Deppeler) Date: Wed Mar 16 03:31:05 2011 Subject: [Histonet] Storing slides in buffer Message-ID: Hi guys, I am wondering if anyone has much experience storing slides in buffer (PBS-T) after retrieval for up to about 48hrs at 4C? Is it likely that they will still stain? Or is it going to be entirely dependent on the epitope? I'm optimizing research antibodies right now and I'd like to decrease my turn around time by knocking off the HIER at an earlier time point. Thanks. -- Stacy Deppeler. Research Assistant Department of Ophthalmology King Abdulaziz University Hospital Riyadh, KSA From felix.nagel <@t> meduniwien.ac.at Wed Mar 16 04:23:51 2011 From: felix.nagel <@t> meduniwien.ac.at (Felix Nagel) Date: Wed Mar 16 04:24:01 2011 Subject: [Histonet] staining of damaged mitochondria Message-ID: Hi, Do you know a way to stain damaged mitochondria in rat hearts after ischemia/reperfusion by immunohistochemistry? Thank you very much! Felix -- Felix Nagel Ludwig Boltzmann Cluster for Cardiovascular Research c/o Core Unit for Biomedical Research Waehringer Guertel 18-20 - Leitstelle 1Q A-1090 Vienna Austria Tel: +43 1 40400 5223 Mail: felix.nagel@meduniwien.ac.at Website: www.cardiovascular-research.at From gvdobbin <@t> ihis.org Wed Mar 16 05:44:03 2011 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Wed Mar 16 05:44:10 2011 Subject: [Histonet] Light hematoxylin staining in H&E Message-ID: <4D806A43020000C80000FC01@smtp1.gov.pe.ca> Are you soaking your blocks in dilute ammonium hydroxide before sectioning? Soaking too long will affect stain quality. Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Jordan, Velma" 03/15/11 22:22 PM >>> We have been troubleshooting complaints of hematoxylin staining light in our H&E. The main complaint is no differentiation between the nucleus and cytoplasm. We are using Richard-Allan hematoxylin ,eosin,clarifier and bluing. We tried surgipath, changing times, pH the H2O and any other thing we could think of, but the problem remains. Does anyone have a suggestion? Velma Jordan UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From BMolinari <@t> heart.thi.tmc.edu Wed Mar 16 05:50:04 2011 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Wed Mar 16 05:51:03 2011 Subject: [Histonet] Bielschowskys Stain In-Reply-To: References: <1C8A72A1-5685-47ED-BE87-07F96F3EF8B6@gmail.com> Message-ID: Glawon, Thank you for your help. I appreciate it. Betsy -----Original Message----- From: Glawon Flood [mailto:smoothw@gmail.com] Sent: Tuesday, March 15, 2011 2:33 PM To: Molinari, Betsy Subject: Re: [Histonet] Bielschowskys Stain Betsy, I don't know of any modifications for Eager's but there are modifications for the Fink-Heimer technique as well as the Nauta-Gygax technique that can be used on paraffin-embedded tissue. I've only come across these in passing and you may have to do a little digging to find a protocol. I hope the info proves useful...good luck! Glawon Flood, HTL (ASCP) Won On Mar 15, 2011, at 11:43 AM, "Molinari, Betsy" wrote: > Thank you for your response. I "googled" Eagers and found a protocol for Eagers for frozen sections. I have paraffin blocks. > Do you know if there is and Eagers method for paraffin? > Thank you so much. > Betsy > > -----Original Message----- > From: Glawon Flood [mailto:smoothw@gmail.com] > Sent: Tuesday, March 15, 2011 10:22 AM > To: Molinari, Betsy > Subject: Re: [Histonet] Bielschowskys Stain > > Hi Betsy, > > Let me start off by saying that I am by no means an expert on what's preferred. However, in my experience, I've come to find the Bielschowsky method very useful for staining nerve fibers, but I would not consider it so for demonstrating axonal degeneration. I would suggest that you maybe consider Eager's method for degenerating axons. Unlike Bielschowsky's, Eager's actually distinguishes those fibers that are normal from those that are degenerating. > > I hope this helps. > > Glawon Flood, HTL (ASCP) > > On Mar 15, 2011, at 6:00 AM, "Molinari, Betsy" wrote: > >> Hi , >> An investigator would like show axonal degeneration in peripheral nerves. Is Bielschowsky's the preferred stain? >> Thanks! >> >> >> Betsy Molinari HT(ASCP) >> Texas Heart Institute >> Cardiovascular Pathology >> 6770 Bertner Ave. >> MC 1-283 >> Houston, TX 77030 >> 832-355-6524 >> 832-355-6812 (fax) >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Wed Mar 16 08:37:22 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Wed Mar 16 08:37:28 2011 Subject: [Histonet] Storing slides in buffer In-Reply-To: References: Message-ID: I just did this last week. I stored them in wash buffer overnight in the frig. and they were fine =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacy Deppeler Sent: Wednesday, March 16, 2011 3:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storing slides in buffer Hi guys, I am wondering if anyone has much experience storing slides in buffer (PBS-T) after retrieval for up to about 48hrs at 4C? Is it likely that they will still stain? Or is it going to be entirely dependent on the epitope? I'm optimizing research antibodies right now and I'd like to decrease my turn around time by knocking off the HIER at an earlier time point. Thanks. -- Stacy Deppeler. Research Assistant Department of Ophthalmology King Abdulaziz University Hospital Riyadh, KSA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sfonner <@t> labpath.com Wed Mar 16 08:51:49 2011 From: sfonner <@t> labpath.com (Sheila Fonner) Date: Wed Mar 16 08:53:32 2011 Subject: [Histonet] CONTROLS Message-ID: <000501cbe3e1$4bfdebb0$e3f9c310$@com> Hello everyone, I have an issue that I would like some help with. We are currently running SMM-HC and E-Cad on Breast cores and specimens from a derm lab. With the regulations being so strict regarding the processing times of breast specimens, how are you all dealing with that when it comes to your control tissue? We have to acquire breast tissue from outside sources to use for control, but it is very hard to get it within that 24-72 hour processing time since many hospitals will not release any tissue until the case has been signed out. Does anyone have any wonderful ideas on how to handle that? I would really appreciate it! Thanks histonetters. Sheila Knoxville Dermatopathology Lab Knoxville, TN From Randi.Hayes <@t> horizonnb.ca Wed Mar 16 09:03:01 2011 From: Randi.Hayes <@t> horizonnb.ca (Hayes, Randi (HorizonNB)) Date: Wed Mar 16 09:03:36 2011 Subject: [Histonet] Procedure for making Gram Control Message-ID: Hello out there in Histoland, I'm looking for a procedure for making your own Gram controls. Any assistance would be appreciated. Thanks, Randi ------- Horizon Health Network Disclaimer -------

This e-mail communication (including any or all attachments) is intended
only for the use of the person or entity to which it is addressed and may
contain confidential and/or privileged material. If you are not the intended
recipient of this e-mail, any use, review, retransmission, distribution,
dissemination, copying, printing, or other use of, or taking of any action in
reliance upon this e-mail, is strictly prohibited. If you have received this
e-mail in error, please contact the sender and delete the original and any
copy of this e-mail and any printout thereof, immediately. Your
co-operation is appreciated.

Le pr?sent courriel (y compris toute pi?ce jointe) s'adresse uniquement ?
son destinataire, qu'il soit une personne ou un organisme, et pourrait
comporter des renseignements privil?gi?s ou confidentiels. Si vous n'?tes
pas le destinataire du courriel, il est interdit d'utiliser, de revoir, de
retransmettre, de distribuer, de diss?miner, de copier ou d'imprimer ce
courriel, d'agir en vous y fiant ou de vous en servir de toute autre fa?on.
Si vous avez re?u le pr?sent courriel par erreur, pri?re de communiquer
avec l'exp?diteur et d'?liminer l'original du courriel, ainsi que toute copie
?lectronique ou imprim?e de celui-ci, imm?diatement. Nous sommes
reconnaissants de votre collaboration.
From wbenton <@t> cua.md  Wed Mar 16 09:07:07 2011
From: wbenton <@t> cua.md (Walter Benton)
Date: Wed Mar 16 09:07:48 2011
Subject: [Histonet] RE: Procedure for making Gram Control
In-Reply-To: 
References: 
Message-ID: <0B8979A204680A42B93A52B486088CD91C6F60F09A@CUAEXH1.GCU-MD.local>

Making you own...not sure how to do that, but Slim Jim's work in a pinch.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wbenton@cua.md
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hayes, Randi (HorizonNB) [Randi.Hayes@horizonnb.ca]
Sent: Wednesday, March 16, 2011 10:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Procedure for making Gram Control

Hello out there in Histoland,

I'm looking for a procedure for making your own Gram controls.  Any
assistance would be appreciated.

Thanks, Randi
------- Horizon Health Network Disclaimer -------

This e-mail communication (including any or all attachments) is intended
only for the use of the person or entity to which it is addressed and may
contain confidential and/or privileged material. If you are not the intended
recipient of this e-mail, any use, review, retransmission, distribution,
dissemination, copying, printing, or other use of, or taking of any action in
reliance upon this e-mail, is strictly prohibited. If you have received this
e-mail in error, please contact the sender and delete the original and any
copy of this e-mail and any printout thereof, immediately. Your
co-operation is appreciated.

Le pr?sent courriel (y compris toute pi?ce jointe) s'adresse uniquement ?
son destinataire, qu'il soit une personne ou un organisme, et pourrait
comporter des renseignements privil?gi?s ou confidentiels. Si vous n'?tes
pas le destinataire du courriel, il est interdit d'utiliser, de revoir, de
retransmettre, de distribuer, de diss?miner, de copier ou d'imprimer ce
courriel, d'agir en vous y fiant ou de vous en servir de toute autre fa?on.
Si vous avez re?u le pr?sent courriel par erreur, pri?re de communiquer
avec l'exp?diteur et d'?liminer l'original du courriel, ainsi que toute copie
?lectronique ou imprim?e de celui-ci, imm?diatement. Nous sommes
reconnaissants de votre collaboration.

CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law.  If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy.

From MLunetta <@t> luhcares.org  Wed Mar 16 13:14:55 2011
From: MLunetta <@t> luhcares.org (Matthew Lunetta)
Date: Wed Mar 16 13:15:07 2011
Subject: [Histonet] PRN Position in Longmont Colorado
Message-ID: <4D80A9BF020000A80005870E@ns.luhcares.org>

Hey all,
There is a PRN position at Longmont United Hospital open. Working Mon-friday no weekends no evenings.
I have attached the link to apply.
http://longmontunitedhospital.force.com/Careers/ts2__JobDetails?jobId=a0IA0000002ct4yMAA&tSource=
thanks
Matt Lunetta HT(ASCP)
Longmont United Hospital
From amario3 <@t> uic.edu  Wed Mar 16 13:57:16 2011
From: amario3 <@t> uic.edu (Andrea Marion)
Date: Wed Mar 16 13:57:21 2011
Subject: [Histonet] Re: Storing slides in buffer
Message-ID: 

It makes sense that some epitopes might be more sensitive than others. For
48 hours, I would probably store in just PBS, and leave out the detergent
(I am assuming the 'T' in your PBST is Tween). Even though Tween is a weak
detergent, I would worry that long-term detergent treatment might dissolve
the cell membranes or solubilize some proteins, rather than just
permeabilizing the membrane.

I've successfully done staining for phospho-serine 10-histone 3, cleaved
caspase 3, and cardiac troponin T after an extra day stored in PBS at 4
degree.


Andrea Marion
Graduate Student
University of Illinois - Chicago
amario3 [at] uic [dot] edu


Hi guys,

I am wondering if anyone has much experience storing slides in buffer
(PBS-T) after retrieval for up to about 48hrs at 4C? Is it likely that they
will still stain? Or is it going to be entirely dependent on the epitope?

I'm optimizing research antibodies right now and I'd like to decrease my
turn around time by knocking off the HIER at an earlier time point.

Thanks.


-- 
Stacy Deppeler.

Research Assistant
Department of Ophthalmology
King Abdulaziz University Hospital
Riyadh, KSA


From k_reek <@t> yahoo.com  Wed Mar 16 14:25:31 2011
From: k_reek <@t> yahoo.com (Katrina Reek)
Date: Wed Mar 16 14:25:35 2011
Subject: [Histonet] Making gram controls
Message-ID: <55004.76460.qm@web63602.mail.re1.yahoo.com>

Randi,
?
I don't have a written procedure to forward to you, but this is what we do when we make our gram controls. We use fresh tissue from an autopsy before it goes in formalin (such as lung, we've used placenta before as well). Then put a couple pieces of the fresh lung into containers labeled gram +, and gram - (if you want two pieces of tissue on your control; if you're Pathologists prefer a single tissue on the control with both bugs in them, the you'd only need one container).We get the next steps done with assistance from our Microbiology department. Pour broth (not sure what they use, can get that for you as well if you need) into each of the two containers and then inoculate the gram + container with the appropriate bacteria. Do the same with the gram -'s. Incubate the containers at 37degree's for 24-48hrs. We then pour formalin into the containers and?let them fix.?Dump the formalin and then put the tissue?in labeled cassette's, process?them,
 embed, and cut them to make sure they?stain properly and both sets of bacteria are present. We then seal the blocks back up with more paraffin.?They seem to last longer that way for us.?
?
I googled the topic, and these were steps I found online - although they were for micro, I altered some steps to fit into working for Histo.
?
Katrina Newell, HT(ASCP)
Crittenton Hospital Medical Center
Rochester, MI 48307
?


      
From akbitting <@t> geisinger.edu  Wed Mar 16 15:41:04 2011
From: akbitting <@t> geisinger.edu (Angela Bitting)
Date: Wed Mar 16 15:41:12 2011
Subject: [Histonet] S100 A1
Message-ID: <4D80E821.2B7F.00C9.1@geisinger.edu>

I'm looking for a mouse anti human S100 A1 antibody for IHC.
 My vendor doesn't carry it anymore.
Anyone have one that they are using on FFPE tissue?
Could you send me ordering info or a copy of your data sheet?
 
Thanks,
Angie
 
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!


IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
-------------- next part --------------
BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:Bitting, Angela
TEL;WORK:570-271-6844
ORG:;Histology
EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu
N:Bitting;Angela
END:VCARD

From ruppert.amysue <@t> marshfieldclinic.org  Wed Mar 16 16:36:00 2011
From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue)
Date: Wed Mar 16 16:36:08 2011
Subject: [Histonet] Light hematoxylin staining in H%26E&In-Reply-To=
Message-ID: <201103162135.p2GLZqpD027974@spamfilt>

We seem to be constantly battling this as well. We have found that the quality of the ethanol used in processing and staining can be an issue. We now purchase ours from a company at www.UltraPure.com. The quality of the DI or distilled water being used can also be an issue. We are currently in the process of getting off of our lab building DI water, and purchasing a Elix Advantage Purification system from Millipore. We also found that the type of Eosin can make a big difference. We have recently changed to Eosin from Anatech, and this gives us a very good end product with our Hematoxylin that we still make from scratch--yes we still make our own:)
 However, the best results we get are when we do not use the DI water that comes from our buildings water system. That is why we are working hard to get our dept our own purification system. It is a very long story how we have come to this decision. But checking into your water supply and the quality of it would be very worthwhile.
 Very few people realize or understand the importance of water quality for the histology lab. 
Good luck
amysue ruppert

______________________________________________________________________
The contents of this message may contain private, protected and/or privileged information.  If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within.  Please contact the sender and advise of the erroneous delivery by return e-mail or telephone.  Thank you for your cooperation.
From mturner <@t> CSILaboratories.com  Wed Mar 16 18:36:15 2011
From: mturner <@t> CSILaboratories.com (Mark Turner)
Date: Wed Mar 16 18:39:05 2011
Subject: [Histonet] CAP checklist, question ANP.23041.
Message-ID: <28FD5AB4A24C8249BDBED1C7FA7C999A023FDAC8@csi-srv-007.CSI-LABS.local>

Regarding CAP checklist, question ANP.23041.  "The operation of the imaging system is performed by high-complexity testing personnel."

 

We have a question regarding the qualifications of the operators.  The operators of the Aperio system are simply scanning entire slides to make a record for us prior to returning slides to the client.  Our operators make no evaluation of the image other than whether the scan is adequate for recording purposes, and this is verified by our medical staff prior to release of the materials.  In your opinion, does this constitute high-complexity testing and require CLIA compliant qualifications for the operators?

Mark Turner, Ph.D, HT(ASCP) QIHC

IHC / Histology Manager

CSI Laboratories

 

770-817-0817 x 394                  

678-205-4669  FAX

mturner@csilaboratories.com  

csilaboratories.com  

11525 Park Woods Circle

Alpharetta, GA 30005

 

Important Warning: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately and delete the related e-mail. 

 

 

 

From macveigh <@t> usc.edu  Wed Mar 16 19:26:29 2011
From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni)
Date: Wed Mar 16 19:26:34 2011
Subject: [Histonet] Research and Clinical Labs
Message-ID: <000901cbe439$f5c5f090$e151d1b0$@usc.edu>

For 10 years I ran Histology lab for a large University. We used the same
equipment and lab for both animal and human tissue. 

I had to certify the lab with CLEA for human tissue at one point (and this
took serious work!). If you are already certified for human, using the more
strict regulations which apply to human tissue, won't hurt the rest, nor
increase in any way your time or effort involvement. You do it anyway. I
wouldn't leave anything marked "for research" unless it has proper label
with exp date etc.  

We received everything already fixed and most of the time in cassettes, so
there was not much grossing.

The processor ran the same night and everything was finished the following
day. No one, during any of the inspections had trouble with this. As far as
I remember, there was no question about this in any of the check lists sent
to me. 

It was 11 years ago when I parted with my Histo lab and I am not current on
the latest regulations, but as far as the technical part... there shouldn't
be a problem. 

 

Michelle Aloni MS, HTL ASCP

USC Keck School of Medicine

From JEllin <@t> yumaregional.org  Wed Mar 16 19:46:01 2011
From: JEllin <@t> yumaregional.org (Jesus Ellin)
Date: Wed Mar 16 19:46:08 2011
Subject: [Histonet] CAP checklist, question ANP.23041.
In-Reply-To: <28FD5AB4A24C8249BDBED1C7FA7C999A023FDAC8@csi-srv-007.CSI-LABS.local>
References: <28FD5AB4A24C8249BDBED1C7FA7C999A023FDAC8@csi-srv-007.CSI-LABS.local>
Message-ID: <29BE166A2CF48D459853F8EC57CD37E8035D6A32@EXCHANGECLUSTER.yumaregional.local>

Mark this is a new question and let me answer this for you from a
inspection point of view.  Currently there are 15 to 18 new questions
that deal with predicative markers and the digital images, most of these
are QA/QC related.  But the issue with images is that your people are
inspecting them.  When you are looking at an image it is more than just
click and go.  There are technical issues of Tiling, focus with z
stacking, AOI ( if the full image was captured), magnification, etc.
This is just technical to images, but then there are the aspects that
are to the histology world as in staining components, microtomy,
fixation, etc.  To say that they don't make a decision is a huge
understatement.  As far as I know CAP put this in because people were
just scanning images and sending them off.  There are also issues were
the scanner scans for possible algorithm studies.  You have to make sure
this is inline.  So as for the High complexity testing to what CLIA
defines, that is a back and forth issue.  Currently within our facility
you have to be licensed HT/HTL to do scanning.  This comes in line with
the CAP looking at creating a QM program and having the
technician/Technologist maintain competencies and assessments for this
technology.  There is also a competency and assessment due for the
pathologist as well for this technology.  I can go on and on, but you
can contact me off line for this.

Jesus Ellin
Yuma Regional Medical Center
928-336-1743

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark
Turner
Sent: Wednesday, March 16, 2011 4:36 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CAP checklist, question ANP.23041.

Regarding CAP checklist, question ANP.23041.  "The operation of the
imaging system is performed by high-complexity testing personnel."

 

We have a question regarding the qualifications of the operators.  The
operators of the Aperio system are simply scanning entire slides to make
a record for us prior to returning slides to the client.  Our operators
make no evaluation of the image other than whether the scan is adequate
for recording purposes, and this is verified by our medical staff prior
to release of the materials.  In your opinion, does this constitute
high-complexity testing and require CLIA compliant qualifications for
the operators?

Mark Turner, Ph.D, HT(ASCP) QIHC

IHC / Histology Manager

CSI Laboratories

 

770-817-0817 x 394                  

678-205-4669  FAX

mturner@csilaboratories.com  

csilaboratories.com



11525 Park Woods Circle

Alpharetta, GA 30005

 

Important Warning: This e-mail is intended for the use of the person to
whom it is addressed and may contain information that is privileged and
confidential, the disclosure of which is governed by applicable law. If
the reader of this e-mail is not the intended recipient, or the employee
or agent responsible to deliver it to the intended recipient, you are
hereby notified that any dissemination, distribution or copying of this
information is STRICTLY PROHIBITED. If you have received this message in
error, please notify us immediately and delete the related e-mail. 

 

 

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

______________________________________________________________________
This message is confidential, intended only for the named 
recipient(s) and may contain information that is privileged 
or exempt from disclosure under applicable law.  If you are 
not the intended recipient(s), you are notified that the 
dissemination, distribution, or copying of this message is 
strictly prohibited.  If you receive this message in error, 
or are not the named recipient(s), please notify the sender 
at either the e-mail, fax, address, or telephone number 
listed above and delete this e-mail from your computer. 
Thank You.
______________________________________________________________________

From marktarango <@t> gmail.com  Wed Mar 16 20:03:03 2011
From: marktarango <@t> gmail.com (Mark Tarango)
Date: Wed Mar 16 20:03:08 2011
Subject: [Histonet] CAP checklist, question ANP.23041.
In-Reply-To: <28FD5AB4A24C8249BDBED1C7FA7C999A023FDAC8@csi-srv-007.CSI-LABS.local>
References: <28FD5AB4A24C8249BDBED1C7FA7C999A023FDAC8@csi-srv-007.CSI-LABS.local>
Message-ID: 

Well I wouldn't try and use a Ph.D. in religous studies to qualify for high
complexity testing...

On Wed, Mar 16, 2011 at 4:36 PM, Mark Turner wrote:

> Regarding CAP checklist, question ANP.23041.  "The operation of the imaging
> system is performed by high-complexity testing personnel."
>
>
>
> We have a question regarding the qualifications of the operators.  The
> operators of the Aperio system are simply scanning entire slides to make a
> record for us prior to returning slides to the client.  Our operators make
> no evaluation of the image other than whether the scan is adequate for
> recording purposes, and this is verified by our medical staff prior to
> release of the materials.  In your opinion, does this constitute
> high-complexity testing and require CLIA compliant qualifications for the
> operators?
>
> Mark Turner, Ph.D, HT(ASCP) QIHC
>
> IHC / Histology Manager
>
> CSI Laboratories
>
>
>
> 770-817-0817 x 394
>
> 678-205-4669  FAX
>
> mturner@csilaboratories.com 
>
> csilaboratories.com <
> https://csi-srv-007/exchweb/bin/redir.asp?URL=http://www.csi-labs.com/>
>
> 11525 Park Woods Circle
>
> Alpharetta, GA 30005
>
>
>
> Important Warning: This e-mail is intended for the use of the person to
> whom it is addressed and may contain information that is privileged and
> confidential, the disclosure of which is governed by applicable law. If the
> reader of this e-mail is not the intended recipient, or the employee or
> agent responsible to deliver it to the intended recipient, you are hereby
> notified that any dissemination, distribution or copying of this information
> is STRICTLY PROHIBITED. If you have received this message in error, please
> notify us immediately and delete the related e-mail.
>
>
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From W.E.J.Hoekert <@t> olvg.nl  Thu Mar 17 03:47:18 2011
From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.)
Date: Thu Mar 17 03:49:09 2011
Subject: [Histonet] RE: Procedure for making Gram Control
References: 
	<0B8979A204680A42B93A52B486088CD91C6F60F09A@CUAEXH1.GCU-MD.local>
Message-ID: <1190CB05C44B13409483514729C2FC360C0B73@PAIT42.olvg.nl>

What would be the equivalent of a Slim Jim in Europe? The Netherlands to be more precise?
 
 

________________________________

Van: histonet-bounces@lists.utsouthwestern.edu namens Walter Benton
Verzonden: wo 16-3-2011 15:07
Aan: Hayes, Randi (HorizonNB); histonet@lists.utsouthwestern.edu
Onderwerp: [Histonet] RE: Procedure for making Gram Control



Making you own...not sure how to do that, but Slim Jim's work in a pinch.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wbenton@cua.md
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hayes, Randi (HorizonNB) [Randi.Hayes@horizonnb.ca]
Sent: Wednesday, March 16, 2011 10:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Procedure for making Gram Control

Hello out there in Histoland,

I'm looking for a procedure for making your own Gram controls.  Any
assistance would be appreciated.

Thanks, Randi
------- Horizon Health Network Disclaimer -------

This e-mail communication (including any or all attachments) is intended
only for the use of the person or entity to which it is addressed and may
contain confidential and/or privileged material. If you are not the intended
recipient of this e-mail, any use, review, retransmission, distribution,
dissemination, copying, printing, or other use of, or taking of any action in
reliance upon this e-mail, is strictly prohibited. If you have received this
e-mail in error, please contact the sender and delete the original and any
copy of this e-mail and any printout thereof, immediately. Your
co-operation is appreciated.

Le pr?sent courriel (y compris toute pi?ce jointe) s'adresse uniquement ?
son destinataire, qu'il soit une personne ou un organisme, et pourrait
comporter des renseignements privil?gi?s ou confidentiels. Si vous n'?tes
pas le destinataire du courriel, il est interdit d'utiliser, de revoir, de
retransmettre, de distribuer, de diss?miner, de copier ou d'imprimer ce
courriel, d'agir en vous y fiant ou de vous en servir de toute autre fa?on.
Si vous avez re?u le pr?sent courriel par erreur, pri?re de communiquer
avec l'exp?diteur et d'?liminer l'original du courriel, ainsi que toute copie
?lectronique ou imprim?e de celui-ci, imm?diatement. Nous sommes
reconnaissants de votre collaboration.

CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law.  If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Disclaimer: 

Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. 
In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend.


From thenoblesavage44 <@t> gmail.com  Thu Mar 17 11:29:58 2011
From: thenoblesavage44 <@t> gmail.com (Britt Tracy)
Date: Thu Mar 17 11:30:05 2011
Subject: [Histonet] re: triple immunofluorescence for apoptosis (blue
	fluorophore?)
Message-ID: 

 Good morning!

I'm looking for advice on an antibody conjugated with a blue fluorophore
that could be used to tag for apoptosis (on p75 receptors on cholinergic
neurons in the rat basal forebrain).

We use a Goat X ChAT primary (1:100) and a Rhodamine-conjugated Donkey X
Goat secondary (1:250) on the slices.

The slices already contain GFP expressed by the viral vector we infused into
the basal forebrain.

So, when we image the sections we already have a green fluorophore and a red
fluorophore. Is there a blue fluorophore tag for apoptosis that anyone has
used which would be biochemically compatible with the antibodies above?


Is there another fluorescent option? Internet-searching and
literature-reading has not gotten me anywhere.

Our scope is a Leica DM4000B equipped with a Leica DFC 425C camera.



Thanks for your time; any advice or insight would be much appreciated.


Sincerely,

Britt Tracy (undergraduate researcher at Temple University)
From cananatacan <@t> live.com  Thu Mar 17 11:33:17 2011
From: cananatacan <@t> live.com (canan atacan)
Date: Thu Mar 17 11:33:22 2011
Subject: [Histonet] paraffin for pathology lab.
In-Reply-To: 
References: 
Message-ID: 


 
Hi all 
 
I have a question. If you spent time and answer this I will be appreciated. 
 
To use in our pathology laboratory we need to get beaded paraffin. I need to order it from companies. 
 
According to my research I understand that the oil content, penetration depth ... important for this product and I dont know what should % of this oil content be?
 
It will effect penetration dept? We need to get 56-58 C melting point parffin. 
 
Please inform 
 
thanks alot
 
Canan 		 	   		  
From arvidsonkristen <@t> yahoo.com  Thu Mar 17 11:49:36 2011
From: arvidsonkristen <@t> yahoo.com (kristen arvidson)
Date: Thu Mar 17 11:49:39 2011
Subject: [Histonet] small derm specimen handling
Message-ID: <264378.90002.qm@web65713.mail.ac4.yahoo.com>

Hello All,
Just wondering how you all treat your small derm specimens as not to lose them in processing.? Does anyone use colored formalin or add dye to the buckets?? We have had a few lost ones lately (more than usual :( )? We use the smaller cassettes from Leica. The problem with those is they trap air bubbles so I hate to use them more than we have to.? Just looking for some new ideas.? Thanks for your input.


      
From sfonner <@t> labpath.com  Thu Mar 17 11:55:48 2011
From: sfonner <@t> labpath.com (Sheila Fonner)
Date: Thu Mar 17 11:57:45 2011
Subject: [Histonet] small derm specimen handling
In-Reply-To: <264378.90002.qm@web65713.mail.ac4.yahoo.com>
References: <264378.90002.qm@web65713.mail.ac4.yahoo.com>
Message-ID: <002f01cbe4c4$2a3ffa70$7ebfef50$@com>

You can use any cassette you like if you put a blue sponge inside of it.
Some people use two sponges, one on top and one on bottom, or you can use
just one and fold it in half.  Another thing you might want to try are the
small biopsy bags.  They are like tiny tea bags and you just pour the
contents of your specimen bottle into the bag.  The formalin will run out
and the specimen will stay trapped inside.  Then you can fold the bag to fit
inside of your cassette.  You can also add some eosin to the specimen at
grossing if it is white or transparent, but it sounds like that's not really
the issue if you are losing them altogether.  Hope this helps!

Sheila
Knoxville Dermatopathology Lab
Knoxville Tennessee


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen
arvidson
Sent: Thursday, March 17, 2011 12:50 PM
To: histonet
Subject: [Histonet] small derm specimen handling

Hello All,
Just wondering how you all treat your small derm specimens as not to lose
them in processing.? Does anyone use colored formalin or add dye to the
buckets?? We have had a few lost ones lately (more than usual :( )? We use
the smaller cassettes from Leica. The problem with those is they trap air
bubbles so I hate to use them more than we have to.? Just looking for some
new ideas.? Thanks for your input.


      
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From AGleiberman <@t> cbiolabs.com  Thu Mar 17 12:15:35 2011
From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman)
Date: Thu Mar 17 12:15:41 2011
Subject: [Histonet] re: triple immunofluorescence for apoptosis
	(bluefluorophore?)
In-Reply-To: 
References: 
Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C15E580@cbiolabs05.CBiolabs.local>

Bill,
There are plenty of secondary conjugated with blue fluorophore (check JacksonImmunoresearch or Invitrogen catalogs), but usually these fluorophores are not very good, signal is dull and long exposure burns signals not only in blue channel, but in red and green as well. If your microscope is equipped with filters for deep red channel you can use Cy5 or DyLight647 from Jackson or AlexaFluor647 from Invitrogen. You will not be able to see the signal by eye, but your digital camera should be able to register it - and leave blue channel for DNA dyes such as DAPI or Hoechst.In this case, you will have four color fluorescence. 

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman@cbiolabs.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Britt Tracy
Sent: Thursday, March 17, 2011 12:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] re: triple immunofluorescence for apoptosis (blue fluorophore?)

 Good morning!

I'm looking for advice on an antibody conjugated with a blue fluorophore
that could be used to tag for apoptosis (on p75 receptors on cholinergic
neurons in the rat basal forebrain).

We use a Goat X ChAT primary (1:100) and a Rhodamine-conjugated Donkey X
Goat secondary (1:250) on the slices.

The slices already contain GFP expressed by the viral vector we infused into
the basal forebrain.

So, when we image the sections we already have a green fluorophore and a red
fluorophore. Is there a blue fluorophore tag for apoptosis that anyone has
used which would be biochemically compatible with the antibodies above?


Is there another fluorescent option? Internet-searching and
literature-reading has not gotten me anywhere.

Our scope is a Leica DM4000B equipped with a Leica DFC 425C camera.



Thanks for your time; any advice or insight would be much appreciated.


Sincerely,

Britt Tracy (undergraduate researcher at Temple University)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

 


This communication may contain privileged information.  It is intended solely for the use of the addressee.  If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information.  If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy.  This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act.  You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information.

From tjasper <@t> copc.net  Thu Mar 17 12:32:22 2011
From: tjasper <@t> copc.net (Thomas Jasper)
Date: Thu Mar 17 12:32:30 2011
Subject: [Histonet] RE: Procedure for making Gram Control
References: 	
	<0B8979A204680A42B93A52B486088CD91C6F60F09A@CUAEXH1.GCU-MD.local> 
	<1190CB05C44B13409483514729C2FC360C0B73@PAIT42.olvg.nl>
Message-ID: <90354A475B420441B2A0396E5008D49695EC50@copc-sbs.COPC.local>

Some sort of small, snack sausage of questionable quality.  I'm not familiar with anything like that in Europe, but maybe you could determine that somehow.
Good Luck!
Tom Jasper
Bend, Oregon


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hoekert, W.E.J.
Sent: Thursday, March 17, 2011 1:47 AM
To: Walter Benton; Hayes, Randi (HorizonNB); histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Procedure for making Gram Control

What would be the equivalent of a Slim Jim in Europe? The Netherlands to be more precise?
 
 

________________________________

Van: histonet-bounces@lists.utsouthwestern.edu namens Walter Benton
Verzonden: wo 16-3-2011 15:07
Aan: Hayes, Randi (HorizonNB); histonet@lists.utsouthwestern.edu
Onderwerp: [Histonet] RE: Procedure for making Gram Control



Making you own...not sure how to do that, but Slim Jim's work in a pinch.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wbenton@cua.md
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hayes, Randi (HorizonNB) [Randi.Hayes@horizonnb.ca]
Sent: Wednesday, March 16, 2011 10:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Procedure for making Gram Control

Hello out there in Histoland,

I'm looking for a procedure for making your own Gram controls.  Any
assistance would be appreciated.

Thanks, Randi
------- Horizon Health Network Disclaimer -------

This e-mail communication (including any or all attachments) is intended
only for the use of the person or entity to which it is addressed and may
contain confidential and/or privileged material. If you are not the intended
recipient of this e-mail, any use, review, retransmission, distribution,
dissemination, copying, printing, or other use of, or taking of any action in
reliance upon this e-mail, is strictly prohibited. If you have received this
e-mail in error, please contact the sender and delete the original and any
copy of this e-mail and any printout thereof, immediately. Your
co-operation is appreciated.

Le pr?sent courriel (y compris toute pi?ce jointe) s'adresse uniquement ?
son destinataire, qu'il soit une personne ou un organisme, et pourrait
comporter des renseignements privil?gi?s ou confidentiels. Si vous n'?tes
pas le destinataire du courriel, il est interdit d'utiliser, de revoir, de
retransmettre, de distribuer, de diss?miner, de copier ou d'imprimer ce
courriel, d'agir en vous y fiant ou de vous en servir de toute autre fa?on.
Si vous avez re?u le pr?sent courriel par erreur, pri?re de communiquer
avec l'exp?diteur et d'?liminer l'original du courriel, ainsi que toute copie
?lectronique ou imprim?e de celui-ci, imm?diatement. Nous sommes
reconnaissants de votre collaboration.

CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law.  If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Disclaimer: 

Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. 
In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From Mike.Manzer <@t> pathlogic.com  Thu Mar 17 12:46:27 2011
From: Mike.Manzer <@t> pathlogic.com (Mike Manzer)
Date: Thu Mar 17 12:47:22 2011
Subject: [Histonet] Histotechnician Job Announcement
Message-ID: 

Are you the type of person who has the persistence and follow through to master the art of Histology? Do you have great interpersonal skills to solve problems and learn with others?  Do you have an eye for detail and accuracy?  

Path Logic, an expanding pathology service located in Carmichael, has an immediate opening for a full time Histotechnician. The job requires skills, knowledge and abilities to gross, embed, section and stain various types of tissue. Knowledge of special stains and immunohistochemistry is preferred. Other duties include the operation and maintenance of tissue processors, microtomes, autostainers, coverslippers, and routine lab equipment; documentation and monitoring of quality control systems; and adherence to lab safety and HIPPA requirements.

This position requires at least two years of Histology experience, and eligibility for ASCP certification as a Histotechnician or Histotechnologist. 

Path Logic offers excellent pay and benefits in a friendly work environment.   

E-mail resume and salary requirements to Mike.Manzer@PathLogic.com

Michael Manzer, HTL
Histology Supervisor
Path Logic
Carmichael, CA
(916) 692-7164



From jaylundgren <@t> gmail.com  Thu Mar 17 12:57:04 2011
From: jaylundgren <@t> gmail.com (Jay Lundgren)
Date: Thu Mar 17 12:57:08 2011
Subject: [Histonet] paraffin for pathology lab.
In-Reply-To: 
References: 
	
Message-ID: 

     As long as you order a quality paraffin from a reputable laboratory
supply company, you should be alright.  I've used just about every paraffin
out there, and they are all adequate.  Some are better than others, and
everyone has their personal preference.  My personal favorite is Paraplast
Plus, which has added DMSO for better infiltration.


                                                                 Sincerely,

                                                                     Jay A.
Lundgren M.S., HTL (ASCP)
From chak_bou <@t> yahoo.com  Thu Mar 17 12:57:42 2011
From: chak_bou <@t> yahoo.com (Chakib Boussahmain)
Date: Thu Mar 17 12:57:47 2011
Subject: [Histonet] Sectioning Chinchilla's stomach
Message-ID: <737807.13201.qm@web161801.mail.bf1.yahoo.com>

Hi Histoneters,
I am encountering a problems when I am sectionong Chinchilla's stomach tissues( formalin fixed paraffin embedded). Sections desintegrate, shred and scratch and they roll up tightly.
Does anyone experienced something like this? I will appreciate any input.
Thank you
Chakib Boussahmain
Histology Supervisor HTL(ASCP)
MIT


      
From sfonner <@t> labpath.com  Thu Mar 17 13:08:52 2011
From: sfonner <@t> labpath.com (Sheila Fonner)
Date: Thu Mar 17 13:09:13 2011
Subject: [Histonet] small derm specimen handling
In-Reply-To: 
References: <264378.90002.qm@web65713.mail.ac4.yahoo.com>
	<002f01cbe4c4$2a3ffa70$7ebfef50$@com>
	
Message-ID: <000a01cbe4ce$5f728e10$1e57aa30$@com>

Rena,

We just use the good old VIP from Sakura.  We are a dermpath lab and do up
to 600 blocks a day.  Some of our specimens are very tiny.  I would check at
the grossing point first, make sure that the cassettes are being properly
closed.  They have to latch tightly, or they can open during processing and
you will lose specimens.  The next place I would check is at the embedding
station.  Do your embedders wear gloves?  Sometimes when you open cassettes,
small specimens can stick to either your gloves or your fingers and you
don't even realize it.  Also, check the lids before throwing them away.  If
the lids are popped off and tossed in the trash can with the assumption that
the specimen is inside the cassette, you will lose it in the trash can and
may never find it.  Derms are tricky.  With a little TLC you can learn to do
"miracles" with them, but you have to remember that they are small, can
hide, and can flip out very easily.  If you are using sponges and bags
inside the cassettes, I doubt they are coming out during processing.  Go
through all the steps and "prove" to everyone how easily accidents can
happen.  It's really an issue of checks and balances between ALL who handle
that specimen.  Make sure embedders are using some type of embedding log so
that they can check how many pieces should be in the cassette as soon as
they open it.  Only open one at a time for a while.  I've noticed people
opening ten or twenty and pieces can stick to your labcoat sleeve, your
hand, almost anything that passes over that open cassette.  Try to find the
source of your problem and you will be able to fix it!  

Regards,
Sheila


-----Original Message-----
From: Bouchal, Rena L [mailto:bouchalr@wvuhealthcare.com] 
Sent: Thursday, March 17, 2011 1:57 PM
To: Sheila Fonner
Subject: RE: [Histonet] small derm specimen handling

Sheila... what processor are you using please? We have been having problems
with lost tissue- in spite of using sponges, bags, wrapping it, etc, and we
are beginning to question our newer processor..

Please note that my email address  as of Jan 3, 2011 is
bouchalr@wvuhealthcare.com  .   Please make the appropriate changes in your
address book.

Rena Bouchal, M.S.
Anatomic Pathology Manager
West Virginia University Hospitals
304-293-7765

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila
Fonner
Sent: Thursday, March 17, 2011 12:56 PM
To: 'kristen arvidson'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] small derm specimen handling

You can use any cassette you like if you put a blue sponge inside of it.
Some people use two sponges, one on top and one on bottom, or you can use
just one and fold it in half.  Another thing you might want to try are the
small biopsy bags.  They are like tiny tea bags and you just pour the
contents of your specimen bottle into the bag.  The formalin will run out
and the specimen will stay trapped inside.  Then you can fold the bag to fit
inside of your cassette.  You can also add some eosin to the specimen at
grossing if it is white or transparent, but it sounds like that's not really
the issue if you are losing them altogether.  Hope this helps!

Sheila
Knoxville Dermatopathology Lab
Knoxville Tennessee


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen
arvidson
Sent: Thursday, March 17, 2011 12:50 PM
To: histonet
Subject: [Histonet] small derm specimen handling

Hello All,
Just wondering how you all treat your small derm specimens as not to lose
them in processing.? Does anyone use colored formalin or add dye to the
buckets?? We have had a few lost ones lately (more than usual :( )? We use
the smaller cassettes from Leica. The problem with those is they trap air
bubbles so I hate to use them more than we have to.? Just looking for some
new ideas.? Thanks for your input.


      
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
-----------------------------------------
Confidentiality Notice: This e-mail message, including any
attachments, is for the sole use of the intended recipient(s) and
may contain confidential and privileged information.  Any
unauthorized review, use, disclosure or distribution is prohibited.
 If you are not the intended recipient, please contact the sender
by reply e-mail and destroy all copies of the original message.



From AlieteE_Langsdorf <@t> DFCI.HARVARD.EDU  Thu Mar 17 13:31:39 2011
From: AlieteE_Langsdorf <@t> DFCI.HARVARD.EDU (Langsdorf, Aliete E.)
Date: Thu Mar 17 13:31:44 2011
Subject: [Histonet] EZ-TMA Kit?
Message-ID: <064301362827D243B0F0D3B63E70906305644C5F@PHSXMB24.partners.org>

Hi, 

Has anyone used the EZ-TMA Kit for making your own tissue micro arrays?
I am trying to decide on which system to buy. So far, Quick-Ray and Arraymold
seem to be the favorites, but I would like to hear if anyone has tried EZ-TMA
before I make a decision.

Thanks!
~Ally Langsdorf

Senior Research Technician
Medical Oncology
Dana-Farber Cancer Institute





The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.
From AHutton <@t> dh.org  Thu Mar 17 13:48:46 2011
From: AHutton <@t> dh.org (Hutton, Allison)
Date: Thu Mar 17 13:50:52 2011
Subject: [Histonet] glass coverslippers
Message-ID: <38A56C4F4630D348A50B3720409270870E0FE314@dhmail.dhorg.org>

I am in the market for a new coverslipper.  I am looking at Thermo's clearvue or Leica's CV 5030.  Does anyone have any input on either of these coverslippers, good or bad.
Thank you in advance,
Allison
From christiegowan <@t> msn.com  Thu Mar 17 14:02:13 2011
From: christiegowan <@t> msn.com (CHRISTIE GOWAN)
Date: Thu Mar 17 14:02:19 2011
Subject: [Histonet] Dako Coverstainer System
Message-ID: 


Is anyone using this stainer and what are your thoughts? I have concerns about the reagents being so close to the ground. This stainer also locks you into purchasing Dako hematoxylin, bluing and eosin. Thanks.
Christie 		 	   		  
From BSullivan <@t> shorememorial.org  Thu Mar 17 14:12:45 2011
From: BSullivan <@t> shorememorial.org (BSullivan@shorememorial.org)
Date: Thu Mar 17 14:13:20 2011
Subject: [Histonet] glass coverslippers
In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE314@dhmail.dhorg.org>
Message-ID: 

I have used the new Leica coverslipper. I am not a fan of this
coverslipper. Too many variables with the mountant. We use what is
recommended and we either have too much and it goes on top of the coverslip
or not enough and we have air bubbles. They have been out to adjust and
still we have issues.  We have a total Leica lab and I have to say I would
not recommend this piece of equipment. One thing they did not say at the
time of purchase................it will only coverslip their Immuno slides.
If you attempt to use it for Ventana stained slides you have your hands
full because of the labels.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


Speak only well of people and you need never whisper


                                                                           
             "Hutton, Allison"                                             
                                                           
             Sent by:                                                   To 
             histonet-bounces@          
             lists.utsouthwest                                          cc 
             ern.edu                                                       
                                                                   Subject 
                                       [Histonet] glass coverslippers      
             03/17/2011 02:48                                              
             PM                                                            
                                                                           
                                                                           
                                                                           
                                                                           




I am in the market for a new coverslipper.  I am looking at Thermo's
clearvue or Leica's CV 5030.  Does anyone have any input on either of these
coverslippers, good or bad.
Thank you in advance,
Allison
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From TGoins <@t> mt.gov  Thu Mar 17 14:16:09 2011
From: TGoins <@t> mt.gov (Goins, Tresa)
Date: Thu Mar 17 14:16:14 2011
Subject: [Histonet] paraffin for pathology lab.
In-Reply-To: 
References: 
	
Message-ID: 

It sounds like you are looking for paraffin for infiltration (not embedding).  If this is so, use a paraffin with shorter polymers like Richard-Allan Paraffin Type 1 - The same company makes Types 3, 6, and 9 but these longer polymer paraffins are designed for sectioning and ribbon formation.



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of canan atacan
Sent: Thursday, March 17, 2011 10:33 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] paraffin for pathology lab.


 
Hi all 
 
I have a question. If you spent time and answer this I will be appreciated. 
 
To use in our pathology laboratory we need to get beaded paraffin. I need to order it from companies. 
 
According to my research I understand that the oil content, penetration depth ... important for this product and I dont know what should % of this oil content be?
 
It will effect penetration dept? We need to get 56-58 C melting point parffin. 
 
Please inform 
 
thanks alot
 
Canan 		 	   		  
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From trathborne <@t> somerset-healthcare.com  Thu Mar 17 14:19:12 2011
From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni)
Date: Thu Mar 17 14:20:43 2011
Subject: [Histonet] glass coverslippers
In-Reply-To: 
Message-ID: 

We have this coverslipper too. Although we do not have a Ventana ihc stainer, we sometimes coverslip our H & E slides with labels on. Once the instrument was adjusted for a different slide thickness, there was no problem.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
BSullivan@shorememorial.org
Sent: Thursday, March 17, 2011 3:13 PM
To: Hutton, Allison
Cc: histonet@lists.utsouthwestern.edu;
histonet-bounces@lists.utsouthwestern.edu
Subject: Re: [Histonet] glass coverslippers


I have used the new Leica coverslipper. I am not a fan of this
coverslipper. Too many variables with the mountant. We use what is
recommended and we either have too much and it goes on top of the coverslip
or not enough and we have air bubbles. They have been out to adjust and
still we have issues.  We have a total Leica lab and I have to say I would
not recommend this piece of equipment. One thing they did not say at the
time of purchase................it will only coverslip their Immuno slides.
If you attempt to use it for Ventana stained slides you have your hands
full because of the labels.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


Speak only well of people and you need never whisper


                                                                           
             "Hutton, Allison"                                             
                                                           
             Sent by:                                                   To 
             histonet-bounces@          
             lists.utsouthwest                                          cc 
             ern.edu                                                       
                                                                   Subject 
                                       [Histonet] glass coverslippers      
             03/17/2011 02:48                                              
             PM                                                            
                                                                           
                                                                           
                                                                           
                                                                           




I am in the market for a new coverslipper.  I am looking at Thermo's
clearvue or Leica's CV 5030.  Does anyone have any input on either of these
coverslippers, good or bad.
Thank you in advance,
Allison
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee.  The information contained in this
message is confidential and may contain privileged, confidential,
proprietary and/or trade secret information entitled to protection and/or
exemption from disclosure under applicable law.  Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
at 908-685-2200, ext. 4050.

Be sure to visit Somerset Medical Center's Web site - 
www.somersetmedicalcenter.com - for the most up-to-date news, 
event listings, health information and more.
From HornHV <@t> archildrens.org  Thu Mar 17 14:22:57 2011
From: HornHV <@t> archildrens.org (Horn, Hazel V)
Date: Thu Mar 17 14:23:03 2011
Subject: [Histonet] CAP checklist, question ANP.23041.
In-Reply-To: 
References: <28FD5AB4A24C8249BDBED1C7FA7C999A023FDAC8@csi-srv-007.CSI-LABS.local>
	
Message-ID: <25A4DE08332B19499904459F00AAACB7194516A76C@EVS1.archildrens.org>

A bit catty?

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Wednesday, March 16, 2011 8:03 PM
To: Mark Turner
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] CAP checklist, question ANP.23041.

Well I wouldn't try and use a Ph.D. in religous studies to qualify for high
complexity testing...

On Wed, Mar 16, 2011 at 4:36 PM, Mark Turner wrote:

> Regarding CAP checklist, question ANP.23041.  "The operation of the imaging
> system is performed by high-complexity testing personnel."
>
>
>
> We have a question regarding the qualifications of the operators.  The
> operators of the Aperio system are simply scanning entire slides to make a
> record for us prior to returning slides to the client.  Our operators make
> no evaluation of the image other than whether the scan is adequate for
> recording purposes, and this is verified by our medical staff prior to
> release of the materials.  In your opinion, does this constitute
> high-complexity testing and require CLIA compliant qualifications for the
> operators?
>
> Mark Turner, Ph.D, HT(ASCP) QIHC
>
> IHC / Histology Manager
>
> CSI Laboratories
>
>
>
> 770-817-0817 x 394
>
> 678-205-4669  FAX
>
> mturner@csilaboratories.com 
>
> csilaboratories.com <
> https://csi-srv-007/exchweb/bin/redir.asp?URL=http://www.csi-labs.com/>
>
> 11525 Park Woods Circle
>
> Alpharetta, GA 30005
>
>
>
> Important Warning: This e-mail is intended for the use of the person to
> whom it is addressed and may contain information that is privileged and
> confidential, the disclosure of which is governed by applicable law. If the
> reader of this e-mail is not the intended recipient, or the employee or
> agent responsible to deliver it to the intended recipient, you are hereby
> notified that any dissemination, distribution or copying of this information
> is STRICTLY PROHIBITED. If you have received this message in error, please
> notify us immediately and delete the related e-mail.
>
>
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

******************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************
The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the 
intended recipient, or an employee or agent responsible for delivering this 
message to the intended recipient, you are hereby notified that any 
dissemination, distribution or copying of this communication is strictly 
prohibited. If you have received this communication in error, please notify 
us immediately by replying to the message and deleting it from your computer.
Thank you.

From MSHERWOOD <@t> PARTNERS.ORG  Thu Mar 17 14:23:53 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Thu Mar 17 14:23:57 2011
Subject: [Histonet] glass coverslippers
In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE314@dhmail.dhorg.org>
References: <38A56C4F4630D348A50B3720409270870E0FE314@dhmail.dhorg.org>
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5581@PHSXMB30.partners.org>

Allison,

We have a refurbished Leica CV5030 and do like it.  However, I agree with
Beatrice, it took many hours of adjusting the drop size, and the ratio of
mounting media with sovlent, to solve the mounting media problem.  We did end up
buying a smaller needle to dispense the mounting media and since then we have
not had any problems.  

Leica does not sell this size needle.  If anyone would like the ordering
information, I would be glad to to send the info to you.

Peggy
P.S.  I have no experience with any other coverslipper (our histology dept. uses
the Leica). 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison
Sent: Thursday, March 17, 2011 2:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] glass coverslippers

I am in the market for a new coverslipper.  I am looking at Thermo's clearvue or
Leica's CV 5030.  Does anyone have any input on either of these coverslippers,
good or bad.
Thank you in advance,
Allison
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


From sbreeden <@t> nmda.nmsu.edu  Thu Mar 17 14:40:44 2011
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Thu Mar 17 14:40:47 2011
Subject: [Histonet] glass coverslippers
In-Reply-To: 
References: <38A56C4F4630D348A50B3720409270870E0FE314@dhmail.dhorg.org>
	
Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47720@nmdamailsvr.nmda.ad.nmsu.edu>

I agree - I have a totally Leica lab except for my Sakura tape
coverslipper and I would not trade it for anything.  I test-drove the
Leica glass but it seemed that there were more minute ("my-noot")
adjustments than for the Space Station and I could see problems down the
line (sorry, Leica!).   I know some love tape and some hate it but it's
the absolute easiest to use and adjusting for drip (xylene) and length
of coverslip (for those pesky Ventana-labeled slides) is a breeze. My
bosses have no issues with optical quality or photography with the tape,
either.   Tape:  Hoo-rah!

From MSHERWOOD <@t> PARTNERS.ORG  Thu Mar 17 14:54:39 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Thu Mar 17 14:54:47 2011
Subject: [Histonet] Reusable Needles for Leica CV5030
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5582@PHSXMB30.partners.org>

The needles we ordered for our Leica CV5030:

The company is Intellispense-www.dispensinglink.com

They sell all sizes of re-usable (metal) luer-lok needles.  We ordered the
following:

Cat. #9991258-5    23 gauge x 1/2"   (pk of 12) - can't recall the price, very
cheap.

Peggy



Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org 




The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.
From MSHERWOOD <@t> PARTNERS.ORG  Thu Mar 17 14:57:01 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Thu Mar 17 14:57:09 2011
Subject: [Histonet] Processing animal fat
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5583@PHSXMB30.partners.org>

My question is directed specifically to veterinary histologists or histologists
who also do a fair amount of animal processing.  We are having a terrible time
processing pig fat.  We had problems previously, but thought we had solved them.
This latest project (pig skin with a lot of fat attached) came out awful.  The
fat was not adequately processed:  couldn't section it, it just crumbled.  In
the block, it appears white and crumbly.  The funny thing is some blocks came
out all right, but most didn't.

PLEASE help!  Let me know how you process your animal fat (sp. Pig)! Is there a
size issue (we trim it if it is greater than 5mm)?  We have gotten help from the
histonet before and instituted these suggestions (i.e. let sit in formalin for
48 hours; put in cassettes in a 60 degree oven for 1-2 hours and then cool to
room temp. Then process using a 1 hour program).

Any help would be appreciated.

Thanks!
Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org 




The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


From PAMarcum <@t> uams.edu  Thu Mar 17 15:01:57 2011
From: PAMarcum <@t> uams.edu (Marcum, Pamela A)
Date: Thu Mar 17 15:02:01 2011
Subject: [Histonet] Processing animal fat
In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5583@PHSXMB30.partners.org>
References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5583@PHSXMB30.partners.org>
Message-ID: 

How large are your specimens?  How thick is the fat layer?  Pam Marcum

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret 
Sent: Thursday, March 17, 2011 2:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Processing animal fat

My question is directed specifically to veterinary histologists or histologists
who also do a fair amount of animal processing.  We are having a terrible time
processing pig fat.  We had problems previously, but thought we had solved them.
This latest project (pig skin with a lot of fat attached) came out awful.  The
fat was not adequately processed:  couldn't section it, it just crumbled.  In
the block, it appears white and crumbly.  The funny thing is some blocks came
out all right, but most didn't.

PLEASE help!  Let me know how you process your animal fat (sp. Pig)! Is there a
size issue (we trim it if it is greater than 5mm)?  We have gotten help from the
histonet before and instituted these suggestions (i.e. let sit in formalin for
48 hours; put in cassettes in a 60 degree oven for 1-2 hours and then cool to
room temp. Then process using a 1 hour program).

Any help would be appreciated.

Thanks!
Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org 




The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice: This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain
confidential and privileged information.  Any unauthorized review,
use, disclosure or distribution is prohibited.  If you are not the 
intended recipient, please contact the sender by reply
e-mail and destroy all copies of the original message..


From liz <@t> premierlab.com  Thu Mar 17 15:02:32 2011
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Thu Mar 17 15:02:36 2011
Subject: [Histonet] Processing animal fat
In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5583@PHSXMB30.partners.org>
Message-ID: 

5mm is pretty thick still.  We process skin and fat and we use a longer
processing cycle.  1 to 1.5 hours per station.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Sherwood, Margaret 
Sent: Thursday, March 17, 2011 1:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Processing animal fat

My question is directed specifically to veterinary histologists or
histologists
who also do a fair amount of animal processing.  We are having a
terrible time
processing pig fat.  We had problems previously, but thought we had
solved them.
This latest project (pig skin with a lot of fat attached) came out
awful.  The
fat was not adequately processed:  couldn't section it, it just
crumbled.  In
the block, it appears white and crumbly.  The funny thing is some blocks
came
out all right, but most didn't.

PLEASE help!  Let me know how you process your animal fat (sp. Pig)! Is
there a
size issue (we trim it if it is greater than 5mm)?  We have gotten help
from the
histonet before and instituted these suggestions (i.e. let sit in
formalin for
48 hours; put in cassettes in a 60 degree oven for 1-2 hours and then
cool to
room temp. Then process using a 1 hour program).

Any help would be appreciated.

Thanks!
Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org 




The information in this e-mail is intended only for the person to whom
it is
addressed. If you believe this e-mail was sent to you in error and the
e-mail
contains patient information, please contact the Partners Compliance
HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you
in error
but does not contain patient information, please contact the sender and
properly
dispose of the e-mail.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From barbie3222 <@t> comcast.net  Thu Mar 17 15:03:08 2011
From: barbie3222 <@t> comcast.net (Barbara Medina)
Date: Thu Mar 17 15:03:15 2011
Subject: [Histonet] please unsubscribe me
Message-ID: 

I have sent this e-mail numerous times. Please unsubscribe me at this time. Thank You
From mturner <@t> CSILaboratories.com  Thu Mar 17 15:06:30 2011
From: mturner <@t> CSILaboratories.com (Mark Turner)
Date: Thu Mar 17 15:06:51 2011
Subject: [Histonet] CAP checklist, question ANP.23041.
In-Reply-To: <25A4DE08332B19499904459F00AAACB7194516A76C@EVS1.archildrens.org>
References: <28FD5AB4A24C8249BDBED1C7FA7C999A023FDAC8@csi-srv-007.CSI-LABS.local>
	
	<25A4DE08332B19499904459F00AAACB7194516A76C@EVS1.archildrens.org>
Message-ID: <28FD5AB4A24C8249BDBED1C7FA7C999A023EBC78@csi-srv-007.CSI-LABS.local>

Just to clear the record, I do qualify for high-complexity testing,  My
doctorate is in another field, and I am very proud of my accomplishments
in both areas of interest.

Thanks for all the private emails and constructive remarks regarding
this question.  They have been very helpful. The consensus seems to be
that there is no consensus.... 

Mark Turner, HT(ASCP) QIHC

-----Original Message-----
From: Horn, Hazel V [mailto:HornHV@archildrens.org] 
Sent: Thursday, March 17, 2011 3:23 PM
To: 'Mark Tarango'; Mark Turner
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] CAP checklist, question ANP.23041.

A bit catty?

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark
Tarango
Sent: Wednesday, March 16, 2011 8:03 PM
To: Mark Turner
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] CAP checklist, question ANP.23041.

Well I wouldn't try and use a Ph.D. in religous studies to qualify for
high
complexity testing...

On Wed, Mar 16, 2011 at 4:36 PM, Mark Turner
wrote:

> Regarding CAP checklist, question ANP.23041.  "The operation of the
imaging
> system is performed by high-complexity testing personnel."
>
>
>
> We have a question regarding the qualifications of the operators.  The
> operators of the Aperio system are simply scanning entire slides to
make a
> record for us prior to returning slides to the client.  Our operators
make
> no evaluation of the image other than whether the scan is adequate for
> recording purposes, and this is verified by our medical staff prior to
> release of the materials.  In your opinion, does this constitute
> high-complexity testing and require CLIA compliant qualifications for
the
> operators?
>
> Mark Turner, Ph.D, HT(ASCP) QIHC
>
> IHC / Histology Manager
>
> CSI Laboratories
>
>
>
> 770-817-0817 x 394
>
> 678-205-4669  FAX
>
> mturner@csilaboratories.com 
>
> csilaboratories.com <
>
https://csi-srv-007/exchweb/bin/redir.asp?URL=http://www.csi-labs.com/>
>
> 11525 Park Woods Circle
>
> Alpharetta, GA 30005
>
>
>
> Important Warning: This e-mail is intended for the use of the person
to
> whom it is addressed and may contain information that is privileged
and
> confidential, the disclosure of which is governed by applicable law.
If the
> reader of this e-mail is not the intended recipient, or the employee
or
> agent responsible to deliver it to the intended recipient, you are
hereby
> notified that any dissemination, distribution or copying of this
information
> is STRICTLY PROHIBITED. If you have received this message in error,
please
> notify us immediately and delete the related e-mail.
>
>
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

************************************************************************
************************************************************************
************************************************************************
************************************************************************
************************************************************************
************************************************************************
************************************************************************
************************************************************************
******************************************************
The information contained in this message may be privileged and
confidential
and protected from disclosure. If the reader of this message is not the 
intended recipient, or an employee or agent responsible for delivering
this 
message to the intended recipient, you are hereby notified that any 
dissemination, distribution or copying of this communication is strictly

prohibited. If you have received this communication in error, please
notify 
us immediately by replying to the message and deleting it from your
computer.
Thank you.

From MSHERWOOD <@t> PARTNERS.ORG  Thu Mar 17 15:08:47 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Thu Mar 17 15:08:51 2011
Subject: [Histonet] Processing animal fat
In-Reply-To: 
References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5583@PHSXMB30.partners.org>
	
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5585@PHSXMB30.partners.org>

The specimens can be large, but we trim so the thickness is less than 5mm. 

-----Original Message-----
From: Marcum, Pamela A [mailto:PAMarcum@uams.edu] 
Sent: Thursday, March 17, 2011 4:02 PM
To: Sherwood, Margaret ; histonet@lists.utsouthwestern.edu
Subject: RE: Re: [Histonet] Processing animal fat

How large are your specimens?  How thick is the fat layer?  Pam Marcum

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood,
Margaret 
Sent: Thursday, March 17, 2011 2:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Processing animal fat

My question is directed specifically to veterinary histologists or histologists
who also do a fair amount of animal processing.  We are having a terrible time
processing pig fat.  We had problems previously, but thought we had solved them.
This latest project (pig skin with a lot of fat attached) came out awful.  The
fat was not adequately processed:  couldn't section it, it just crumbled.  In
the block, it appears white and crumbly.  The funny thing is some blocks came
out all right, but most didn't.

PLEASE help!  Let me know how you process your animal fat (sp. Pig)! Is there a
size issue (we trim it if it is greater than 5mm)?  We have gotten help from the
histonet before and instituted these suggestions (i.e. let sit in formalin for
48 hours; put in cassettes in a 60 degree oven for 1-2 hours and then cool to
room temp. Then process using a 1 hour program).

Any help would be appreciated.

Thanks!
Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org 




The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice: This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain
confidential and privileged information.  Any unauthorized review,
use, disclosure or distribution is prohibited.  If you are not the 
intended recipient, please contact the sender by reply
e-mail and destroy all copies of the original message..


From rjbuesa <@t> yahoo.com  Thu Mar 17 15:08:55 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Mar 17 15:08:59 2011
Subject: [Histonet] Processing animal fat
In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5583@PHSXMB30.partners.org>
Message-ID: <906451.91670.qm@web65714.mail.ac4.yahoo.com>

If you use a mixture of 2-propanol and mineral oil followed by pure mineral oil to "clear" before infiltrating with paraffin, you will be able to cut any type of fatty tissue.
Ren? J.

--- On Thu, 3/17/11, Sherwood, Margaret  wrote:


From: Sherwood, Margaret 
Subject: Re: [Histonet] Processing animal fat
To: histonet@lists.utsouthwestern.edu
Date: Thursday, March 17, 2011, 3:57 PM


My question is directed specifically to veterinary histologists or histologists
who also do a fair amount of animal processing.? We are having a terrible time
processing pig fat.? We had problems previously, but thought we had solved them.
This latest project (pig skin with a lot of fat attached) came out awful.? The
fat was not adequately processed:? couldn't section it, it just crumbled.? In
the block, it appears white and crumbly.? The funny thing is some blocks came
out all right, but most didn't.

PLEASE help!? Let me know how you process your animal fat (sp. Pig)! Is there a
size issue (we trim it if it is greater than 5mm)?? We have gotten help from the
histonet before and instituted these suggestions (i.e. let sit in formalin for
48 hours; put in cassettes in a 60 degree oven for 1-2 hours and then cool to
room temp. Then process using a 1 hour program).

Any help would be appreciated.

Thanks!
Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org 




The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
From algranth <@t> email.arizona.edu  Thu Mar 17 15:09:50 2011
From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth))
Date: Thu Mar 17 15:10:35 2011
Subject: [Histonet] Processing animal fat
In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5583@PHSXMB30.partners.org>
References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5583@PHSXMB30.partners.org>
Message-ID: <9D66307E-B1EA-440C-B515-48507186EB8A@email.arizona.edu>

Peggy,
Is your whole program one hour or is each station one hour?

We had a project here a few years ago that we called "the bacon project" because we had whole chunks of pig skin with an implant and lots of fat in between the layers. It was fixed for a couple days in 10% NBF and then processed with one hour for each station starting in 70% ETOH and ending in 4 paraffins on a VIP. Turned out beautiful. Did trichromes and they were stunning - red, white and blue! The sections were big - about 2.5 cm square - took almost all of the space in the cassette.

Andi


On Mar 17, 2011, at 12:57 PM, Sherwood, Margaret wrote:

> My question is directed specifically to veterinary histologists or histologists
> who also do a fair amount of animal processing.  We are having a terrible time
> processing pig fat.  We had problems previously, but thought we had solved them.
> This latest project (pig skin with a lot of fat attached) came out awful.  The
> fat was not adequately processed:  couldn't section it, it just crumbled.  In
> the block, it appears white and crumbly.  The funny thing is some blocks came
> out all right, but most didn't.
> 
> PLEASE help!  Let me know how you process your animal fat (sp. Pig)! Is there a
> size issue (we trim it if it is greater than 5mm)?  We have gotten help from the
> histonet before and instituted these suggestions (i.e. let sit in formalin for
> 48 hours; put in cassettes in a 60 degree oven for 1-2 hours and then cool to
> room temp. Then process using a 1 hour program).
> 
> Any help would be appreciated.
> 
> Thanks!
> Peggy
> 
> Peggy Sherwood
> Lab Associate, Photopathology
> Wellman Center for Photomedicine (EDR 214)
> Massachusetts General Hospital
> 55 Fruit Street
> Boston, MA 02114-2696
> 617-724-4839 (voice mail)
> 617-726-6983 (lab)
> 617-726-1206 (fax)
> msherwood@partners.org 
> 
> 
> 
> 
> The information in this e-mail is intended only for the person to whom it is
> addressed. If you believe this e-mail was sent to you in error and the e-mail
> contains patient information, please contact the Partners Compliance HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in error
> but does not contain patient information, please contact the sender and properly
> dispose of the e-mail.
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From mucram11 <@t> comcast.net  Thu Mar 17 15:11:08 2011
From: mucram11 <@t> comcast.net (Pamela Marcum)
Date: Thu Mar 17 15:11:18 2011
Subject: [Histonet] paraffin for pathology lab.
In-Reply-To: <1585041394.941139.1300392511393.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net>
Message-ID: <2041260506.941246.1300392668795.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net>

A number of companies selling histology/pathology paraffins are listed on line or in catalogs.? It will depend on what you are actually wanting as these have been developed for routine use and the questions you have asked are taken care by the companies.? I would be happy to give you a list of paraffins I have used or know others have used successfully for years.? Pam Marcum (UAMS) 



----- Original Message ----- 
From: "Tresa Goins " < TGoins @mt.gov> 
To: " canan atacan " < cananatacan @live.com>, histonet @lists. utsouthwestern . edu 
Sent: Thursday, March 17, 2011 2:16:09 PM 
Subject: RE: [ Histonet ] paraffin for pathology lab. 

It sounds like you are looking for paraffin for infiltration (not embedding). ?If this is so, use a paraffin with shorter polymers like Richard-Allan Paraffin Type 1 - The same company makes Types 3, 6, and 9 but these longer polymer paraffins are designed for sectioning and ribbon formation. 



-----Original Message----- 
From: histonet -bounces@lists. utsouthwestern . edu [ mailto : histonet -bounces@lists. utsouthwestern . edu ] On Behalf Of canan atacan 
Sent: Thursday, March 17, 2011 10:33 AM 
To: histonet @lists. utsouthwestern . edu 
Subject: [ Histonet ] paraffin for pathology lab. 


? 
Hi all 
? 
I have a question. If you spent time and answer this I will be appreciated. 
? 
To use in our pathology laboratory we need to get beaded paraffin. I need to order it from companies. 
? 
According to my research I understand that the oil content, penetration depth ... important for this product and I dont know what should % of this oil content be? 
? 
It will effect penetration dept? We need to get 56-58 C melting point parffin . 
? 
Please inform 
? 
thanks alot 
? 
Canan ???????????????? ???????? ? ???????????????? ? 
_______________________________________________ 
Histonet mailing list 
Histonet @lists. utsouthwestern . edu 
http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet 


_______________________________________________ 
Histonet mailing list 
Histonet @lists. utsouthwestern . edu 
http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet 
From shu-cheng.chen <@t> merck.com  Thu Mar 17 15:19:22 2011
From: shu-cheng.chen <@t> merck.com (Chen, Shu-Cheng)
Date: Thu Mar 17 15:19:31 2011
Subject: [Histonet] storage of 10% NBF 
Message-ID: <5D62649615FAA6478F801A08D10E51855A8B6A3446@USCTMXP51003.merck.com>

Hi,

We are asked by our safety officer to find out what the common practice is to store 10% NBF. Is it in the chemical hood only or solvent bunkers? Can the un-opened jars be stored in regular cabinet or on bench top? As far as I know formalin is not flammable. Your experience and knowledge in this area are appreciated.

Thanks,
Shu-cheng
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
From MSHERWOOD <@t> PARTNERS.ORG  Thu Mar 17 15:28:53 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Thu Mar 17 15:29:02 2011
Subject: [Histonet] Processing animal fat
In-Reply-To: <9D66307E-B1EA-440C-B515-48507186EB8A@email.arizona.edu>
References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5583@PHSXMB30.partners.org>
	<9D66307E-B1EA-440C-B515-48507186EB8A@email.arizona.edu>
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5589@PHSXMB30.partners.org>

Every station is 1 hour, and our processing program is similar to yours:  we run
the progam overnight, so specimens sit in formalin for a delayed start.  Then
50%, 70% for 30 min. ea.  Then 2x95%, 3x100%, 2xCitriSolv all for 1 hour.  We
end in one change of paraffin. Our specimens are similar in size to yours,
except we keep the thickness below 5mm.

Any thoughts?  Most of our experimental pig skin/fat are laser treated. Could
that be a problem? 

Peggy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea
L - (algranth)
Sent: Thursday, March 17, 2011 4:10 PM
To: HISTONET
Subject: Re: [Histonet] Processing animal fat

Peggy,
Is your whole program one hour or is each station one hour?

We had a project here a few years ago that we called "the bacon project" because
we had whole chunks of pig skin with an implant and lots of fat in between the
layers. It was fixed for a couple days in 10% NBF and then processed with one
hour for each station starting in 70% ETOH and ending in 4 paraffins on a VIP.
Turned out beautiful. Did trichromes and they were stunning - red, white and
blue! The sections were big - about 2.5 cm square - took almost all of the space
in the cassette.

Andi


On Mar 17, 2011, at 12:57 PM, Sherwood, Margaret wrote:

> My question is directed specifically to veterinary histologists or
histologists
> who also do a fair amount of animal processing.  We are having a terrible time
> processing pig fat.  We had problems previously, but thought we had solved
them.
> This latest project (pig skin with a lot of fat attached) came out awful.  The
> fat was not adequately processed:  couldn't section it, it just crumbled.  In
> the block, it appears white and crumbly.  The funny thing is some blocks came
> out all right, but most didn't.
> 
> PLEASE help!  Let me know how you process your animal fat (sp. Pig)! Is there
a
> size issue (we trim it if it is greater than 5mm)?  We have gotten help from
the
> histonet before and instituted these suggestions (i.e. let sit in formalin for
> 48 hours; put in cassettes in a 60 degree oven for 1-2 hours and then cool to
> room temp. Then process using a 1 hour program).
> 
> Any help would be appreciated.
> 
> Thanks!
> Peggy
> 
> Peggy Sherwood
> Lab Associate, Photopathology
> Wellman Center for Photomedicine (EDR 214)
> Massachusetts General Hospital
> 55 Fruit Street
> Boston, MA 02114-2696
> 617-724-4839 (voice mail)
> 617-726-6983 (lab)
> 617-726-1206 (fax)
> msherwood@partners.org 
> 
> 
> 
> 
> The information in this e-mail is intended only for the person to whom it is
> addressed. If you believe this e-mail was sent to you in error and the e-mail
> contains patient information, please contact the Partners Compliance HelpLine
at
> http://www.partners.org/complianceline . If the e-mail was sent to you in
error
> but does not contain patient information, please contact the sender and
properly
> dispose of the e-mail.
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From MSHERWOOD <@t> PARTNERS.ORG  Thu Mar 17 15:29:54 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Thu Mar 17 15:30:02 2011
Subject: [Histonet] storage of 10% NBF 
In-Reply-To: <5D62649615FAA6478F801A08D10E51855A8B6A3446@USCTMXP51003.merck.com>
References: <5D62649615FAA6478F801A08D10E51855A8B6A3446@USCTMXP51003.merck.com>
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB558A@PHSXMB30.partners.org>

We buy a cubitainer of 10% Buffered formalin and keep on the benchtop for
dispensing. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chen, Shu-Cheng
Sent: Thursday, March 17, 2011 4:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] storage of 10% NBF 

Hi,

We are asked by our safety officer to find out what the common practice is to
store 10% NBF. Is it in the chemical hood only or solvent bunkers? Can the
un-opened jars be stored in regular cabinet or on bench top? As far as I know
formalin is not flammable. Your experience and knowledge in this area are
appreciated.

Thanks,
Shu-cheng
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


From shive003 <@t> umn.edu  Thu Mar 17 15:33:14 2011
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Thu Mar 17 15:33:18 2011
Subject: [Histonet] Processing animal fat
References: 
Message-ID: <4A8ABF70DF634EA69CBD3395C30D2403@auxs.umn.edu>

We trim to 2mm thick.

Jan Shivers
UMN Vet Diag Lab

----- Original Message ----- 
From: "Liz Chlipala" 
To: "Sherwood, Margaret " ; 

Sent: Thursday, March 17, 2011 3:02 PM
Subject: RE: [Histonet] Processing animal fat


5mm is pretty thick still.  We process skin and fat and we use a longer
processing cycle.  1 to 1.5 hours per station.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com


Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Sherwood, Margaret
Sent: Thursday, March 17, 2011 1:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Processing animal fat

My question is directed specifically to veterinary histologists or
histologists
who also do a fair amount of animal processing.  We are having a
terrible time
processing pig fat.  We had problems previously, but thought we had
solved them.
This latest project (pig skin with a lot of fat attached) came out
awful.  The
fat was not adequately processed:  couldn't section it, it just
crumbled.  In
the block, it appears white and crumbly.  The funny thing is some blocks
came
out all right, but most didn't.

PLEASE help!  Let me know how you process your animal fat (sp. Pig)! Is
there a
size issue (we trim it if it is greater than 5mm)?  We have gotten help
from the
histonet before and instituted these suggestions (i.e. let sit in
formalin for
48 hours; put in cassettes in a 60 degree oven for 1-2 hours and then
cool to
room temp. Then process using a 1 hour program).

Any help would be appreciated.

Thanks!
Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org




The information in this e-mail is intended only for the person to whom
it is
addressed. If you believe this e-mail was sent to you in error and the
e-mail
contains patient information, please contact the Partners Compliance
HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you
in error
but does not contain patient information, please contact the sender and
properly
dispose of the e-mail.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From liz <@t> premierlab.com  Thu Mar 17 15:47:14 2011
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Thu Mar 17 15:47:17 2011
Subject: [Histonet] Processing animal fat
In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5589@PHSXMB30.partners.org>
Message-ID: 

The laser treatment should not be an issue, we do that type of work all
of the time.  I would trim to about 2-3 mm.  I did notice that you only
have 2 changes of citrisolv a xylene substitute I have not worked with
that one in particular but if I do work with a xylene substitute we use
three changes and we also have 3 to 4 changes of paraffin depending upon
sample size.  For dermis and skin we have a tendency to process longer
but have never experienced any over processing or "cooked" samples. 

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Sherwood, Margaret 
Sent: Thursday, March 17, 2011 2:29 PM
To: Grantham, Andrea L - (algranth); HISTONET
Subject: RE: [Histonet] Processing animal fat

Every station is 1 hour, and our processing program is similar to yours:
we run
the progam overnight, so specimens sit in formalin for a delayed start.
Then
50%, 70% for 30 min. ea.  Then 2x95%, 3x100%, 2xCitriSolv all for 1
hour.  We
end in one change of paraffin. Our specimens are similar in size to
yours,
except we keep the thickness below 5mm.

Any thoughts?  Most of our experimental pig skin/fat are laser treated.
Could
that be a problem? 

Peggy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Grantham, Andrea
L - (algranth)
Sent: Thursday, March 17, 2011 4:10 PM
To: HISTONET
Subject: Re: [Histonet] Processing animal fat

Peggy,
Is your whole program one hour or is each station one hour?

We had a project here a few years ago that we called "the bacon project"
because
we had whole chunks of pig skin with an implant and lots of fat in
between the
layers. It was fixed for a couple days in 10% NBF and then processed
with one
hour for each station starting in 70% ETOH and ending in 4 paraffins on
a VIP.
Turned out beautiful. Did trichromes and they were stunning - red, white
and
blue! The sections were big - about 2.5 cm square - took almost all of
the space
in the cassette.

Andi


On Mar 17, 2011, at 12:57 PM, Sherwood, Margaret wrote:

> My question is directed specifically to veterinary histologists or
histologists
> who also do a fair amount of animal processing.  We are having a
terrible time
> processing pig fat.  We had problems previously, but thought we had
solved
them.
> This latest project (pig skin with a lot of fat attached) came out
awful.  The
> fat was not adequately processed:  couldn't section it, it just
crumbled.  In
> the block, it appears white and crumbly.  The funny thing is some
blocks came
> out all right, but most didn't.
> 
> PLEASE help!  Let me know how you process your animal fat (sp. Pig)!
Is there
a
> size issue (we trim it if it is greater than 5mm)?  We have gotten
help from
the
> histonet before and instituted these suggestions (i.e. let sit in
formalin for
> 48 hours; put in cassettes in a 60 degree oven for 1-2 hours and then
cool to
> room temp. Then process using a 1 hour program).
> 
> Any help would be appreciated.
> 
> Thanks!
> Peggy
> 
> Peggy Sherwood
> Lab Associate, Photopathology
> Wellman Center for Photomedicine (EDR 214)
> Massachusetts General Hospital
> 55 Fruit Street
> Boston, MA 02114-2696
> 617-724-4839 (voice mail)
> 617-726-6983 (lab)
> 617-726-1206 (fax)
> msherwood@partners.org 
> 
> 
> 
> 
> The information in this e-mail is intended only for the person to whom
it is
> addressed. If you believe this e-mail was sent to you in error and the
e-mail
> contains patient information, please contact the Partners Compliance
HelpLine
at
> http://www.partners.org/complianceline . If the e-mail was sent to you
in
error
> but does not contain patient information, please contact the sender
and
properly
> dispose of the e-mail.
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From shu-cheng.chen <@t> merck.com  Thu Mar 17 15:52:11 2011
From: shu-cheng.chen <@t> merck.com (Chen, Shu-Cheng)
Date: Thu Mar 17 15:52:14 2011
Subject: [Histonet] storage of 10% NBF 
In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB558A@PHSXMB30.partners.org>
References: <5D62649615FAA6478F801A08D10E51855A8B6A3446@USCTMXP51003.merck.com>
	<073AE2BEA1C2BA4A8837AB6C4B943D9708DB558A@PHSXMB30.partners.org>
Message-ID: <5D62649615FAA6478F801A08D10E51855A8B6A34C3@USCTMXP51003.merck.com>

Thank you! We have been doing it the same way. But our new safety officer thinks it is unsafe........

Shu-Cheng 

-----Original Message-----
From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] 
Sent: Thursday, March 17, 2011 4:30 PM
To: Chen, Shu-Cheng; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] storage of 10% NBF 

We buy a cubitainer of 10% Buffered formalin and keep on the benchtop for
dispensing. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chen, Shu-Cheng
Sent: Thursday, March 17, 2011 4:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] storage of 10% NBF 

Hi,

We are asked by our safety officer to find out what the common practice is to
store 10% NBF. Is it in the chemical hood only or solvent bunkers? Can the
un-opened jars be stored in regular cabinet or on bench top? As far as I know
formalin is not flammable. Your experience and knowledge in this area are
appreciated.

Thanks,
Shu-cheng
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


From liz <@t> premierlab.com  Thu Mar 17 16:02:35 2011
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Thu Mar 17 16:02:39 2011
Subject: [Histonet] storage of 10% NBF 
In-Reply-To: <5D62649615FAA6478F801A08D10E51855A8B6A34C3@USCTMXP51003.merck.com>
Message-ID: 

Newcomer supply has a cool container for those 10% NBF 5 gallon cubes if
your safety officer is still hounding you about it.

SAFE CUBE DISPENSER 
Workstation for filling containers with 10% formalin, xylene, alcohols,
lab reagents, detergents, disinfectants, etc. No more uncontrolled drips
or spills. Liquid Detection Alarm in main cabinet & tray. Secondary
containment for up to 5 gals. Great for gross room, morgue, O.R., or
labor and delivery. 20" Deep x 18.5" Wide x 17" High. Can also be wall
mounted.


Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chen,
Shu-Cheng
Sent: Thursday, March 17, 2011 2:52 PM
To: Sherwood, Margaret ; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] storage of 10% NBF 

Thank you! We have been doing it the same way. But our new safety
officer thinks it is unsafe........

Shu-Cheng 

-----Original Message-----
From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] 
Sent: Thursday, March 17, 2011 4:30 PM
To: Chen, Shu-Cheng; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] storage of 10% NBF 

We buy a cubitainer of 10% Buffered formalin and keep on the benchtop
for
dispensing. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chen,
Shu-Cheng
Sent: Thursday, March 17, 2011 4:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] storage of 10% NBF 

Hi,

We are asked by our safety officer to find out what the common practice
is to
store 10% NBF. Is it in the chemical hood only or solvent bunkers? Can
the
un-opened jars be stored in regular cabinet or on bench top? As far as I
know
formalin is not flammable. Your experience and knowledge in this area
are
appreciated.

Thanks,
Shu-cheng
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you
are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom
it is
addressed. If you believe this e-mail was sent to you in error and the
e-mail
contains patient information, please contact the Partners Compliance
HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you
in error
but does not contain patient information, please contact the sender and
properly
dispose of the e-mail.

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you
are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From amario3 <@t> uic.edu  Thu Mar 17 16:14:04 2011
From: amario3 <@t> uic.edu (Andrea Marion)
Date: Thu Mar 17 16:14:09 2011
Subject: [Histonet] re: triple immunofluorescence for apoptosis (blue
	fluorophore?)
Message-ID: <78d2f57a762bc0c33265bceba52f7b3a.squirrel@webmail.uic.edu>

Hi Britt,

There are many many fluorophores you could choose from, including quite a
few that do not overlap with GFP or rhodamine. Here is a partial list:
http://flowcyt.salk.edu/fluo.html

To set up your staining, you will want to first identify a primary
antibody that labels the apoptotic cells you are interested in. Many
people use cleaved-caspase-3 as a marker of apoptosis. I don't know if
this would work in your situation or not. Don't pick a goat primary, since
your ChAT antibody is from goat. Based on what species your primary
antibody was generated from, you will then want to find a secondary
antibody against the primary species that is conjugated to a fluorophore
whose excitation and emission spectra do not overlap with GFP/rhodamine.
This should be easy to find - check Jackson Immunoresearch for the Dylight
series or Invitrogen for Alexafluors.

Your choice of fluorophore is limited mostly by what filters your
microscope has. You need to check that the filter specifications match the
emission spectra of the fluorophore you want to use. I bet you probably
have a DAPI filter; if so, you could probably use a Pacific Blue
conjugate.

Andrea Marion
Graduate Student
University of Illinois at Chicago


----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Britt
Tracy
Sent: Thursday, March 17, 2011 12:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] re: triple immunofluorescence for apoptosis (blue
fluorophore?)

 Good morning!

I'm looking for advice on an antibody conjugated with a blue fluorophore
that could be used to tag for apoptosis (on p75 receptors on cholinergic
neurons in the rat basal forebrain).

We use a Goat X ChAT primary (1:100) and a Rhodamine-conjugated Donkey X
Goat secondary (1:250) on the slices.

The slices already contain GFP expressed by the viral vector we infused into
the basal forebrain.

So, when we image the sections we already have a green fluorophore and a red
fluorophore. Is there a blue fluorophore tag for apoptosis that anyone has
used which would be biochemically compatible with the antibodies above?


Is there another fluorescent option? Internet-searching and
literature-reading has not gotten me anywhere.

Our scope is a Leica DM4000B equipped with a Leica DFC 425C camera.



Thanks for your time; any advice or insight would be much appreciated.


Sincerely,

Britt Tracy (undergraduate researcher at Temple University)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From shu-cheng.chen <@t> merck.com  Thu Mar 17 16:28:22 2011
From: shu-cheng.chen <@t> merck.com (Chen, Shu-Cheng)
Date: Thu Mar 17 16:28:28 2011
Subject: [Histonet] storage of 10% NBF 
In-Reply-To: 
References: <5D62649615FAA6478F801A08D10E51855A8B6A34C3@USCTMXP51003.merck.com>
	
Message-ID: <5D62649615FAA6478F801A08D10E51855A8B6A3530@USCTMXP51003.merck.com>

Thank you very much, Liz. This sounds like a good remedy to my headache. Will look into it. 

Shu-Cheng 

-----Original Message-----
From: Liz Chlipala [mailto:liz@premierlab.com] 
Sent: Thursday, March 17, 2011 5:03 PM
To: Chen, Shu-Cheng; Sherwood, Margaret ; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] storage of 10% NBF 

Newcomer supply has a cool container for those 10% NBF 5 gallon cubes if
your safety officer is still hounding you about it.

SAFE CUBE DISPENSER 
Workstation for filling containers with 10% formalin, xylene, alcohols,
lab reagents, detergents, disinfectants, etc. No more uncontrolled drips
or spills. Liquid Detection Alarm in main cabinet & tray. Secondary
containment for up to 5 gals. Great for gross room, morgue, O.R., or
labor and delivery. 20" Deep x 18.5" Wide x 17" High. Can also be wall
mounted.


Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chen,
Shu-Cheng
Sent: Thursday, March 17, 2011 2:52 PM
To: Sherwood, Margaret ; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] storage of 10% NBF 

Thank you! We have been doing it the same way. But our new safety
officer thinks it is unsafe........

Shu-Cheng 

-----Original Message-----
From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] 
Sent: Thursday, March 17, 2011 4:30 PM
To: Chen, Shu-Cheng; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] storage of 10% NBF 

We buy a cubitainer of 10% Buffered formalin and keep on the benchtop
for
dispensing. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chen,
Shu-Cheng
Sent: Thursday, March 17, 2011 4:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] storage of 10% NBF 

Hi,

We are asked by our safety officer to find out what the common practice
is to
store 10% NBF. Is it in the chemical hood only or solvent bunkers? Can
the
un-opened jars be stored in regular cabinet or on bench top? As far as I
know
formalin is not flammable. Your experience and knowledge in this area
are
appreciated.

Thanks,
Shu-cheng
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you
are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom
it is
addressed. If you believe this e-mail was sent to you in error and the
e-mail
contains patient information, please contact the Partners Compliance
HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you
in error
but does not contain patient information, please contact the sender and
properly
dispose of the e-mail.

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you
are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


From rlhenshall_powell <@t> yahoo.co.uk  Thu Mar 17 18:24:13 2011
From: rlhenshall_powell <@t> yahoo.co.uk (Rhonda Henshall-Powell)
Date: Thu Mar 17 18:24:18 2011
Subject: [Histonet] Seeking Technical support and Field Application
	Specialists with Histotech training and qualifications
Message-ID: <559620.35384.qm@web29510.mail.ird.yahoo.com>

Hello Histonetters,

An industry-leading IHC/ISH diagnostics company is seeking an in-house Technical Support Representative and Field Application Specialists requiring significant travel:

(A) Inside Technical Support Representative
Provide Technical/Applications Support on product related issues via telephone, web and email communication 

(B) Field Applications Specialist
Provide expert level technical support knowledge of Anatomic Pathology principles to troubleshoot and support instrumentation, software, and train lab personnel on use, optimisation and validation of IHC and ISH reagents.

If interested or know of someone who may be interested, please reply directly to me for more information.

Thank you




      

From laurie.reilly <@t> jcu.edu.au  Thu Mar 17 18:44:38 2011
From: laurie.reilly <@t> jcu.edu.au (Reilly, Laurie)
Date: Thu Mar 17 18:46:10 2011
Subject: [Histonet] Processing animal fat
In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5583@PHSXMB30.partners.org>
References: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5583@PHSXMB30.partners.org>
Message-ID: 

Dear Margaret and fellow histonetters,

The major problem with processing fatty tissues, assuming that they are fixed properly, is that Ethanol is not a good solvent for fat and therefore connot penetrate the tissues completely, so the tissues are inadequately dehydrated.
We have had some success with lipomas by adding a "degreasing" step of xylene into the processing schedule.
70% ethanol
80% ethanol
90% ethanol
95% ethanol
Absolute ethanol
Xylene
Absolute ethanol
Xylene
Xylene
Paraffin
Paraffin
Paraffin
The first Absolute ethanol will dehydrate the tissue to some extent. The next Xylene step will remove most of the fat and then the second Absolute ethanol can complete the dehydration.

A compromise situation that we use routinely is to have Absolute ethanol, 50:50 Absolute ethanol:Xylene, Xylene This is not quite as effective but it is less disruptive to the normal schedule and handles moderately fatty tissues.

            Regards from Townsville, Australia.
                      Laurie.

Mr. Laurie REILLY
Histopathology
School of  Veterinary and Biomedical Sciences
James Cook University
Townsville  Qld.  4811
Australia.

Phone 07 4781 4468

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret 
Sent: Friday, 18 March 2011 5:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Processing animal fat

My question is directed specifically to veterinary histologists or histologists
who also do a fair amount of animal processing.  We are having a terrible time
processing pig fat.  We had problems previously, but thought we had solved them.
This latest project (pig skin with a lot of fat attached) came out awful.  The
fat was not adequately processed:  couldn't section it, it just crumbled.  In
the block, it appears white and crumbly.  The funny thing is some blocks came
out all right, but most didn't.

PLEASE help!  Let me know how you process your animal fat (sp. Pig)! Is there a
size issue (we trim it if it is greater than 5mm)?  We have gotten help from the
histonet before and instituted these suggestions (i.e. let sit in formalin for
48 hours; put in cassettes in a 60 degree oven for 1-2 hours and then cool to
room temp. Then process using a 1 hour program).

Any help would be appreciated.

Thanks!
Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood@partners.org 




The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From W.E.J.Hoekert <@t> olvg.nl  Fri Mar 18 04:40:31 2011
From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.)
Date: Fri Mar 18 04:40:38 2011
Subject: [Histonet] RE: Procedure for making Gram Control
References: 	<0B8979A204680A42B93A52B486088CD91C6F60F09A@CUAEXH1.GCU-MD.local>
	<1190CB05C44B13409483514729C2FC360C0B73@PAIT42.olvg.nl>
	<90354A475B420441B2A0396E5008D49695EC50@copc-sbs.COPC.local>
Message-ID: <1190CB05C44B13409483514729C2FC360C0B76@PAIT42.olvg.nl>

OK! I always thought that a Slim Jim was some kind of candy, but it is a snack sausage. Here in The Netherlands we have Bi-Fi. I will try one of those.
Thanks,
 
Willem Hoekert
Amsterdam, The Netherlands.

________________________________

Van: histonet-bounces@lists.utsouthwestern.edu namens Thomas Jasper
Verzonden: do 17-3-2011 18:32
Aan: Hoekert, W.E.J.
CC: histonet@lists.utsouthwestern.edu
Onderwerp: RE: [Histonet] RE: Procedure for making Gram Control



Some sort of small, snack sausage of questionable quality.  I'm not familiar with anything like that in Europe, but maybe you could determine that somehow.
Good Luck!
Tom Jasper
Bend, Oregon


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hoekert, W.E.J.
Sent: Thursday, March 17, 2011 1:47 AM
To: Walter Benton; Hayes, Randi (HorizonNB); histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Procedure for making Gram Control

What would be the equivalent of a Slim Jim in Europe? The Netherlands to be more precise?



________________________________

Van: histonet-bounces@lists.utsouthwestern.edu namens Walter Benton
Verzonden: wo 16-3-2011 15:07
Aan: Hayes, Randi (HorizonNB); histonet@lists.utsouthwestern.edu
Onderwerp: [Histonet] RE: Procedure for making Gram Control



Making you own...not sure how to do that, but Slim Jim's work in a pinch.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wbenton@cua.md
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hayes, Randi (HorizonNB) [Randi.Hayes@horizonnb.ca]
Sent: Wednesday, March 16, 2011 10:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Procedure for making Gram Control

Hello out there in Histoland,

I'm looking for a procedure for making your own Gram controls.  Any
assistance would be appreciated.

Thanks, Randi
------- Horizon Health Network Disclaimer -------

This e-mail communication (including any or all attachments) is intended
only for the use of the person or entity to which it is addressed and may
contain confidential and/or privileged material. If you are not the intended
recipient of this e-mail, any use, review, retransmission, distribution,
dissemination, copying, printing, or other use of, or taking of any action in
reliance upon this e-mail, is strictly prohibited. If you have received this
e-mail in error, please contact the sender and delete the original and any
copy of this e-mail and any printout thereof, immediately. Your
co-operation is appreciated.

Le pr?sent courriel (y compris toute pi?ce jointe) s'adresse uniquement ?
son destinataire, qu'il soit une personne ou un organisme, et pourrait
comporter des renseignements privil?gi?s ou confidentiels. Si vous n'?tes
pas le destinataire du courriel, il est interdit d'utiliser, de revoir, de
retransmettre, de distribuer, de diss?miner, de copier ou d'imprimer ce
courriel, d'agir en vous y fiant ou de vous en servir de toute autre fa?on.
Si vous avez re?u le pr?sent courriel par erreur, pri?re de communiquer
avec l'exp?diteur et d'?liminer l'original du courriel, ainsi que toute copie
?lectronique ou imprim?e de celui-ci, imm?diatement. Nous sommes
reconnaissants de votre collaboration.

CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law.  If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Disclaimer:

Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen.
In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Disclaimer: 

Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. 
In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend.


From lpwenk <@t> sbcglobal.net  Fri Mar 18 04:56:01 2011
From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk)
Date: Fri Mar 18 04:56:02 2011
Subject: [Histonet] Procedure for making Gram Control
In-Reply-To: 
References: 
Message-ID: <38E0D620C1C540B39242DFE130991AD7@HP2010>

Microorganisms: The Perfect Control
JOH, Vol. 4, #4, Dec. 1981

Method for Producing Artificial Spirochete Control Material
JOH Vol. 9, #4, Dec. 1986

Efficient, Inexpensive Method for Making Gram Stain Control Blocks
JOH Vol. 30, #1, Mar. 2007

(JOH = Journal of Histotechnology, from NSH. www.nsh.org  )

Basically, what Katrina said on 3/16. We've used this technique for years.

Our pathologists prefer the Gram + and the Gram - bacteria to be in two 
separate pieces.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

--------------------------------------------------
From: "Hayes, Randi (HorizonNB)" 
Sent: Wednesday, March 16, 2011 10:03 AM
To: 
Subject: [Histonet] Procedure for making Gram Control

>
> Hello out there in Histoland,
>
> I'm looking for a procedure for making your own Gram controls.  Any
> assistance would be appreciated.
>
> Thanks, Randi
>
------- Horizon Health Network Disclaimer -------

This e-mail > communication (including any or all attachments) is intended
only for > the use of the person or entity to which it is addressed and > may
contain confidential and/or privileged material. If you are not the > intended
recipient of this e-mail, any use, review, retransmission, > distribution,
dissemination, copying, printing, or other use of, or > taking of any action in
reliance upon this e-mail, is strictly > prohibited. If you have received this
e-mail in error, please contact > the sender and delete the original and any
copy of this e-mail and any > printout thereof, immediately. Your
co-operation is > appreciated.

Le pr?sent courriel (y compris toute pi?ce jointe) > s'adresse uniquement ?
son destinataire, qu'il soit une personne ou un > organisme, et pourrait
comporter des renseignements privil?gi?s ou > confidentiels. Si vous n'?tes
pas le destinataire du courriel, il est > interdit d'utiliser, de revoir, de
retransmettre, de distribuer, de > diss?miner, de copier ou d'imprimer ce
courriel, d'agir en vous y fiant > ou de vous en servir de toute autre fa?on.
Si vous avez re?u le pr?sent > courriel par erreur, pri?re de communiquer
avec l'exp?diteur et > d'?liminer l'original du courriel, ainsi que toute copie
?lectronique > ou imprim?e de celui-ci, imm?diatement. Nous sommes
reconnaissants de > votre collaboration.
>



> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From rsrichmond <@t> gmail.com  Fri Mar 18 07:21:43 2011
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Fri Mar 18 07:21:47 2011
Subject: [Histonet] Re: Processing animal fat
Message-ID: 

Peggy Sherwood has been set the daunting task of cutting sections of
pig skin with abundant underlying subcutaneous fat.

I confess my only experience with such material is in the form of
chicharrones (fried pork rinds), but one of the worst of surgical
specimens is similar - skin with underlying fat, in large specimens
from melanoma resections.

I can only advise fixing in neutral buffered formalin until the fixed
tissue is hard enough to gross into very thin sections (no thicker
than a nickel), followed by further overnight fixation in the same
fixative.

I'll let the histotechnologists take it from there!

Bob Richmond
Samurai Pathologist
Knoxville TN

From sbreeden <@t> nmda.nmsu.edu  Fri Mar 18 07:36:38 2011
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Fri Mar 18 07:36:42 2011
Subject: [Histonet] Re: Processing animal fat
In-Reply-To: 
References: 
Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47724@nmdamailsvr.nmda.ad.nmsu.edu>

How about a little time in 10% neutral buffered alcoholic formalin?  Say
a couple hours or more?

From rsrichmond <@t> gmail.com  Fri Mar 18 07:44:43 2011
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Fri Mar 18 07:44:46 2011
Subject: [Histonet] Re: Processing animal fat
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E47724@nmdamailsvr.nmda.ad.nmsu.edu>
References: 
	<4D14F0FC9316DD41972D5F03C070908B02E47724@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: 

On Fri, Mar 18, 2011 at 8:36 AM, Sara Breeden  asked:
>>How about a little time in 10% neutral buffered alcoholic formalin? ?Say a couple hours or more?<<

Good for fat, but alcohol hardens skin - you really want thorough
formaldehyde fixation here. Remember it'll go through alcohols in the
processor.

Bob Richmond
Samurai Pathologist
Knoxville TN

From HornHV <@t> archildrens.org  Fri Mar 18 07:56:38 2011
From: HornHV <@t> archildrens.org (Horn, Hazel V)
Date: Fri Mar 18 07:56:45 2011
Subject: [Histonet] RE: glass coverslippers
In-Reply-To: <38A56C4F4630D348A50B3720409270870E0FE314@dhmail.dhorg.org>
References: <38A56C4F4630D348A50B3720409270870E0FE314@dhmail.dhorg.org>
Message-ID: <25A4DE08332B19499904459F00AAACB7194516A770@EVS1.archildrens.org>

We love our Leica CV 5030.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison
Sent: Thursday, March 17, 2011 1:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] glass coverslippers

I am in the market for a new coverslipper.  I am looking at Thermo's clearvue or Leica's CV 5030.  Does anyone have any input on either of these coverslippers, good or bad.
Thank you in advance,
Allison
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

******************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************
The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the 
intended recipient, or an employee or agent responsible for delivering this 
message to the intended recipient, you are hereby notified that any 
dissemination, distribution or copying of this communication is strictly 
prohibited. If you have received this communication in error, please notify 
us immediately by replying to the message and deleting it from your computer.
Thank you.

From PAMarcum <@t> uams.edu  Fri Mar 18 08:10:25 2011
From: PAMarcum <@t> uams.edu (Marcum, Pamela A)
Date: Fri Mar 18 08:10:29 2011
Subject: [Histonet] RE: glass coverslippers
In-Reply-To: <25A4DE08332B19499904459F00AAACB7194516A770@EVS1.archildrens.org>
References: <38A56C4F4630D348A50B3720409270870E0FE314@dhmail.dhorg.org>
	<25A4DE08332B19499904459F00AAACB7194516A770@EVS1.archildrens.org>
Message-ID: 

I agree with Hazel we love ours also.  Pam

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Friday, March 18, 2011 7:57 AM
To: 'Hutton, Allison'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: glass coverslippers

We love our Leica CV 5030.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison
Sent: Thursday, March 17, 2011 1:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] glass coverslippers

I am in the market for a new coverslipper.  I am looking at Thermo's clearvue or Leica's CV 5030.  Does anyone have any input on either of these coverslippers, good or bad.
Thank you in advance,
Allison
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

******************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************
The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the 
intended recipient, or an employee or agent responsible for delivering this 
message to the intended recipient, you are hereby notified that any 
dissemination, distribution or copying of this communication is strictly 
prohibited. If you have received this communication in error, please notify 
us immediately by replying to the message and deleting it from your computer.
Thank you.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice: This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain
confidential and privileged information.  Any unauthorized review,
use, disclosure or distribution is prohibited.  If you are not the 
intended recipient, please contact the sender by reply
e-mail and destroy all copies of the original message..


From Ronald.Houston <@t> nationwidechildrens.org  Fri Mar 18 09:31:19 2011
From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald)
Date: Fri Mar 18 09:31:28 2011
Subject: [Histonet] Fisher Microprobe
Message-ID: 

If anyone has a used Fisher Microprobe system that is sitting around gathering dust, could you please contact me off-line?

I am in desperate need for an intra-op study we are proposing

Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster



----------------------------------------- Confidentiality Notice:
The following mail message, including any attachments, is for the
sole use of the intended recipient(s) and may contain confidential
and privileged information. The recipient is responsible to
maintain the confidentiality of this information and to use the
information only for authorized purposes. If you are not the
intended recipient (or authorized to receive information for the
intended recipient), you are hereby notified that any review, use,
disclosure, distribution, copying, printing, or action taken in
reliance on the contents of this e-mail is strictly prohibited. If
you have received this communication in error, please notify us
immediately by reply e-mail and destroy all copies of the original
message. Thank you.
From Valerie.Hannen <@t> parrishmed.com  Fri Mar 18 09:37:33 2011
From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie)
Date: Fri Mar 18 09:37:37 2011
Subject: [Histonet] H-PAS and Modified Alcian Blue
Message-ID: <5680DA93771F0C48954CC8D38425E72401AB351D@ISMAIL.parrishmed.local>

Hi folks...
 I have two questions, the first being, can anyone tell me what a
"H-PAS" Stain is??
 
Second question has to do with a Modified Alcian Blue stain that is used
to enhance the intraoperative diagnosis of Sentinel Lymph Node
metastasis in Invasive Lobular Carcinoma. Is anyone doing this stain,
and if so, are you using the conventional Alcian Blue control slide? If
not, what are you using for the control?
 
Thanks in advance for any help that I can get with questions.
 
Valerie Hannen, MLT(ASCP),HTL,SU (FL)
Parrish Medical Center
Titusville, Florida


**************************************************************
"This email is intended solely for the use of the individual to
whom it is addressed and may contain information that is
privileged, confidential or otherwise exempt from disclosure
under applicable law. If the reader of this email is not the
intended recipient or the employee or agent responsible for
delivering the message to the intended recipient, you are
hereby notified that any dissemination, distribution, or
copying of this communication is strictly prohibited. If you
have received this communication in error, please immediately
delete this message. Thank you"
**************************************************************
From CKent <@t> mbhs.org  Fri Mar 18 10:01:56 2011
From: CKent <@t> mbhs.org (CKent@mbhs.org)
Date: Fri Mar 18 10:02:03 2011
Subject: [Histonet] AUTO: Carolyn J Kent is out of the office. (returning
	03/21/2011)
Message-ID: 


I am out of the office until 03/21/2011.

I will respond to your message when I return.


Note: This is an automated response to your message  "Histonet Digest, Vol
88, Issue 23" sent on 3/18/2011 4:43:29 AM.

This is the only notification you will receive while this person is away.


From mwhite <@t> mcleodhealth.org  Fri Mar 18 10:18:49 2011
From: mwhite <@t> mcleodhealth.org (mwhite@mcleodhealth.org)
Date: Fri Mar 18 10:18:53 2011
Subject: [Histonet] patients with alias names
Message-ID: 


Histonetters: We are curious to find out how your facilities  handle
patients with alias names.

At our hospital, patients are somewhat frequently admitted with alias names
in order to protect their privacy and/or safety (i.e., gunshot wounds, gang
crime, etc.) . Usually their pathology is done without us ever knowing that
there was an alias.  So, we have slides and blocks that are labeled with an
incorrect name along with the case number, etc.  If the patient was in our
system previously, they use their existing permanent medical record number.

Upon discharge from our hospital computer system, these patients' medical
records are merged or corrected somehow  with the real name , and the name
is updated in our pathology LIS and our clinical lab LIS.   Occasionally
the discharge is timed in such a way that the path report hasn't yet been
signed out; therefore the slides, blocks, gross description, etc. all
contain an alias name that appears as a discrepancy to the pathologist
( who sees the discharged patient's real name on the header of the typed
gross paperwork.)

Our pathologists are very uncomfortable with the slides and blocks having
the alias name. What if they want to send out these materials to other
facilities for consults?  We don't get any report that shows a summary of
aliases.

 What are your policies and procedures?




Melanie S. White, MT(ASCP)
Laboratory Supervisor, Systems/Anatomic Pathology
McLeod Regional Medical Center
(843) 777-2072



NOTICE: This e-mail message and all attachments transmitted with 
it may contain legally PRIVILEGED and CONFIDENTIAL information 
intended solely for the use of the addressee. If the reader of this 
message is not the intended recipient, you are hereby notified 
that any reading, dissemination, distribution, copying, or other use 
of this message or its attachments is strictly prohibited. If you 
have received this message in error, please notify the sender 
immediately and/or notify the postmaster (postmaster@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You.


From AHutton <@t> dh.org  Fri Mar 18 10:47:14 2011
From: AHutton <@t> dh.org (Hutton, Allison)
Date: Fri Mar 18 10:49:21 2011
Subject: [Histonet] RE: glass coverslippers
In-Reply-To: 
Message-ID: <38A56C4F4630D348A50B3720409270870E0FE316@dhmail.dhorg.org>

I thank everyone for their input.
Allison

-----Original Message-----
From: Marcum, Pamela A [mailto:PAMarcum@uams.edu]
Sent: Friday, March 18, 2011 9:10 AM
To: Horn, Hazel V; Hutton, Allison; histonet@lists.utsouthwestern.edu
Subject: RE: glass coverslippers


I agree with Hazel we love ours also.  Pam

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Friday, March 18, 2011 7:57 AM
To: 'Hutton, Allison'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: glass coverslippers

We love our Leica CV 5030.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison
Sent: Thursday, March 17, 2011 1:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] glass coverslippers

I am in the market for a new coverslipper.  I am looking at Thermo's clearvue or Leica's CV 5030.  Does anyone have any input on either of these coverslippers, good or bad.
Thank you in advance,
Allison
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

******************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************
The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the 
intended recipient, or an employee or agent responsible for delivering this 
message to the intended recipient, you are hereby notified that any 
dissemination, distribution or copying of this communication is strictly 
prohibited. If you have received this communication in error, please notify 
us immediately by replying to the message and deleting it from your computer.
Thank you.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice: This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain
confidential and privileged information.  Any unauthorized review,
use, disclosure or distribution is prohibited.  If you are not the 
intended recipient, please contact the sender by reply
e-mail and destroy all copies of the original message..


From ttruscot <@t> vetmed.wsu.edu  Fri Mar 18 11:02:22 2011
From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom)
Date: Fri Mar 18 11:02:29 2011
Subject: [Histonet] comprehensive list?
Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB3D@CVMMBX.vetmed.wsu.edu>

Hi All, Is there a comprehensive list in one location somewhere that gives information on all or most antibodies on how they work with FFPE or FS or other fixatives, so that we don't have to try to many approaches when we get a new antibody to try. Thanks, Tom T
From michelecarr10 <@t> yahoo.com  Fri Mar 18 11:04:20 2011
From: michelecarr10 <@t> yahoo.com (Michele Carr)
Date: Fri Mar 18 11:04:23 2011
Subject: [Histonet] cell block agar
Message-ID: <404613.90131.qm@web120718.mail.ne1.yahoo.com>

I was wondering if anyone has a technique or method for making agar for cell 
blocks.? I was also looking for manufactured agar and where I can get it from.? 
Thanks in advance!
Kind regards,
Michele Carr HTL ASCP


      
From Nacaela.Johnson <@t> USONCOLOGY.COM  Fri Mar 18 11:30:23 2011
From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela)
Date: Fri Mar 18 11:30:26 2011
Subject: [Histonet] cell block agar
In-Reply-To: <404613.90131.qm@web120718.mail.ne1.yahoo.com>
References: <404613.90131.qm@web120718.mail.ne1.yahoo.com>
Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D5BB@txhous1eb012.uson.usoncology.int>

We use histogel here.  It is very easy and quick to use.   


Thanks,
 
Nacaela Johnson, HTL (ASCP)
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  Nacaela.Johnson@USOncology.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Carr
Sent: Friday, March 18, 2011 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cell block agar

I was wondering if anyone has a technique or method for making agar for cell blocks.? I was also looking for manufactured agar and where I can get it from. Thanks in advance!
Kind regards,
Michele Carr HTL ASCP


      
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From jsjurczak <@t> comcast.net Fri Mar 18 11:30:24 2011 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Fri Mar 18 11:30:33 2011 Subject: [Histonet] Part Time/on call Histology Professional in Chicago Western Suburbs Message-ID: <1286206544.1281075.1300465824650.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Our private outpatient specialty lab has an opening for an on-call part-time second shift and weekend histologist. We are located in the western Chicago suburbs about a mile east of Oak Brook Shopping Center. If interested, please contact Andrea O'Brien at 708-486-0076. Expereienced histologists only, please. Lester Raff, MD Medical Director Uropartners Laboratory 2225 Enterprise Drive? Suite 2511 Westchester, IL 60154 Tel: 708.486.0076 Fax: 708.492.0203 From jsjurczak <@t> comcast.net Fri Mar 18 11:30:24 2011 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Fri Mar 18 11:30:37 2011 Subject: [Histonet] Part Time/on call Histology Professional in Chicago Western Suburbs Message-ID: <1286206544.1281075.1300465824650.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Our private outpatient specialty lab has an opening for an on-call part-time second shift and weekend histologist. We are located in the western Chicago suburbs about a mile east of Oak Brook Shopping Center. If interested, please contact Andrea O'Brien at 708-486-0076. Expereienced histologists only, please. Lester Raff, MD Medical Director Uropartners Laboratory 2225 Enterprise Drive? Suite 2511 Westchester, IL 60154 Tel: 708.486.0076 Fax: 708.492.0203 From Nacaela.Johnson <@t> USONCOLOGY.COM Fri Mar 18 11:31:42 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Fri Mar 18 11:31:45 2011 Subject: [Histonet] comprehensive list? In-Reply-To: <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB3D@CVMMBX.vetmed.wsu.edu> References: <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB3D@CVMMBX.vetmed.wsu.edu> Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260245D5BC@txhous1eb012.uson.usoncology.int> I use the technical memo from the vendor. My Cell Marque rep also gave me a nice list of pretreatments to use their antibodies on the Bond. Thanks, Nacaela Johnson, HTL (ASCP) Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Friday, March 18, 2011 11:02 AM To: Histonet Subject: [Histonet] comprehensive list? Hi All, Is there a comprehensive list in one location somewhere that gives information on all or most antibodies on how they work with FFPE or FS or other fixatives, so that we don't have to try to many approaches when we get a new antibody to try. Thanks, Tom T _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From michelecarr10 <@t> yahoo.com Fri Mar 18 11:49:51 2011 From: michelecarr10 <@t> yahoo.com (Michele Carr) Date: Fri Mar 18 11:49:54 2011 Subject: [Histonet] reagents with no expiration dates Message-ID: <514642.94246.qm@web120716.mail.ne1.yahoo.com> Hi everyone was curious about how to obtain an expiration date for those reagents that don't come with one. I know this is a cap requirement and we are preparing for our first inspection soon. Any info would be appreciated. Thank you all, Michele Carr HTL ASCP Medical Laboratory Services From akbitting <@t> geisinger.edu Fri Mar 18 11:54:04 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Mar 18 11:54:09 2011 Subject: [Histonet] reagents with no expiration dates In-Reply-To: <514642.94246.qm@web120716.mail.ne1.yahoo.com> References: <514642.94246.qm@web120716.mail.ne1.yahoo.com> Message-ID: <4D8355EC.2B7F.00C9.1@geisinger.edu> Call the vendor. Many times they will give you a date based upon the lot number. >>> Michele Carr 3/18/2011 12:49 PM >>> Hi everyone was curious about how to obtain an expiration date for those reagents that don't come with one. I know this is a cap requirement and we are preparing for our first inspection soon. Any info would be appreciated. Thank you all, Michele Carr HTL ASCP Medical Laboratory Services _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From rjbuesa <@t> yahoo.com Fri Mar 18 12:29:45 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 18 12:29:48 2011 Subject: [Histonet] comprehensive list? In-Reply-To: <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB3D@CVMMBX.vetmed.wsu.edu> Message-ID: <535303.82446.qm@web65701.mail.ac4.yahoo.com> Try your DAKO representative. NOVOCASTRA also used to have such a list for their Abs. Ren? J. --- On Fri, 3/18/11, Truscott, Tom wrote: From: Truscott, Tom Subject: [Histonet] comprehensive list? To: "Histonet" Date: Friday, March 18, 2011, 12:02 PM Hi All, Is there a comprehensive list in one location somewhere that gives information on all or most antibodies on how they work with FFPE or FS or other fixatives, so that we don't have to try to many approaches when we get a new antibody to try. Thanks, Tom T _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Mar 18 12:33:15 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Mar 18 12:33:21 2011 Subject: [Histonet] recruiter Message-ID: Is there a recruiter that is local to the LA/Inland Empire area? Thanks, Jennifer Jennifer MacDonald Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 274-4884 jmacdonald@mtsac.edu From Timothy.Morken <@t> ucsfmedctr.org Fri Mar 18 12:37:39 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Mar 18 12:37:51 2011 Subject: [Histonet] RE: comprehensive list? In-Reply-To: <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB3D@CVMMBX.vetmed.wsu.edu> References: <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB3D@CVMMBX.vetmed.wsu.edu> Message-ID: Tom, Vendor datasheets are the best source of information. The vast majority of clinical vendors antibodies are designed for formalin fixed paraffin embedded tissue. Research vendors are all over the map and often only test by western blots or elisa so they may or may not have information about how their antibodies work in fixed tissue. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Friday, March 18, 2011 9:02 AM To: Histonet Subject: [Histonet] comprehensive list? Hi All, Is there a comprehensive list in one location somewhere that gives information on all or most antibodies on how they work with FFPE or FS or other fixatives, so that we don't have to try to many approaches when we get a new antibody to try. Thanks, Tom T _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Fri Mar 18 13:08:59 2011 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Fri Mar 18 13:09:04 2011 Subject: [Histonet] RE: comprehensive list? In-Reply-To: References: <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB3D@CVMMBX.vetmed.wsu.edu> Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB6E@CVMMBX.vetmed.wsu.edu> Yes, vendor sheets are good, but not all give enough info, and you may have to compare several vendor, and as you say, antibodies only tested by western blot are common in research. Yet, it would be a great money and timesaver if there was one site to go to, to see if anyone had tried these antibodies by different methods and could also submit their own results for entry, possibly with links to Journal articles, much like what some vendors do. It could be a quick reference guide to setting up some research or double staining methods, or just see if some new request is going to fit your routine. Someone would have to have more time and computer skills than I. Thanks all for the input, TOM T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, March 18, 2011 10:38 AM To: Histonet Subject: [Histonet] RE: comprehensive list? Tom, Vendor datasheets are the best source of information. The vast majority of clinical vendors antibodies are designed for formalin fixed paraffin embedded tissue. Research vendors are all over the map and often only test by western blots or elisa so they may or may not have information about how their antibodies work in fixed tissue. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Friday, March 18, 2011 9:02 AM To: Histonet Subject: [Histonet] comprehensive list? Hi All, Is there a comprehensive list in one location somewhere that gives information on all or most antibodies on how they work with FFPE or FS or other fixatives, so that we don't have to try to many approaches when we get a new antibody to try. Thanks, Tom T _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Mar 18 13:16:35 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Mar 18 13:16:48 2011 Subject: [Histonet] RE: comprehensive list? In-Reply-To: <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB6E@CVMMBX.vetmed.wsu.edu> References: <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB3D@CVMMBX.vetmed.wsu.edu> <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB6E@CVMMBX.vetmed.wsu.edu> Message-ID: Tom, No one is collecting detailed info from a lot of people's experiences, if that is what you want. Probably the closest you can get is at nordiqc site (wwww.nordiqc.org, protein atlas). They test a lot of antibodies and give some info on use and stain pattern but are by no means always reliable and certainly have not tested all antibodies. IHC World is another site that has some info, but not very organized in the terms you mention. Your database idea sounds great. Are you planning on starting one?!?!! Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: Truscott, Tom [mailto:ttruscot@vetmed.wsu.edu] Sent: Friday, March 18, 2011 11:09 AM To: Morken, Timothy; Histonet Subject: RE: comprehensive list? Yes, vendor sheets are good, but not all give enough info, and you may have to compare several vendor, and as you say, antibodies only tested by western blot are common in research. Yet, it would be a great money and timesaver if there was one site to go to, to see if anyone had tried these antibodies by different methods and could also submit their own results for entry, possibly with links to Journal articles, much like what some vendors do. It could be a quick reference guide to setting up some research or double staining methods, or just see if some new request is going to fit your routine. Someone would have to have more time and computer skills than I. Thanks all for the input, TOM T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, March 18, 2011 10:38 AM To: Histonet Subject: [Histonet] RE: comprehensive list? Tom, Vendor datasheets are the best source of information. The vast majority of clinical vendors antibodies are designed for formalin fixed paraffin embedded tissue. Research vendors are all over the map and often only test by western blots or elisa so they may or may not have information about how their antibodies work in fixed tissue. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Friday, March 18, 2011 9:02 AM To: Histonet Subject: [Histonet] comprehensive list? Hi All, Is there a comprehensive list in one location somewhere that gives information on all or most antibodies on how they work with FFPE or FS or other fixatives, so that we don't have to try to many approaches when we get a new antibody to try. Thanks, Tom T _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Fri Mar 18 14:06:19 2011 From: shive003 <@t> umn.edu (shive003@umn.edu) Date: Fri Mar 18 14:06:22 2011 Subject: [Histonet] RE: comprehensive list? In-Reply-To: <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB6E@CVMMBX.vetmed.wsu.edu> References: <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB3D@CVMMBX.vetmed.wsu.edu> <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB6E@CVMMBX.vetmed.wsu.edu> Message-ID: I've also found that occasionally the antigen retrieval method quoted on a vendor's data sheet doesn't unmask antigens as well as another method tried in my initial antibody validation trials with that antibody. So, my point is... any comprehensive list should be used only as a guideline; results in your lab will be affected by the reagents, equipment, and environmental conditions you employ in your own lab. Jan Shivers UMN Vet Diag Lab On Mar 18 2011, Truscott, Tom wrote: Yes, vendor sheets are good, but not all give enough info, and you may have to compare several vendor, and as you say, antibodies only tested by western blot are common in research. Yet, it would be a great money and timesaver if there was one site to go to, to see if anyone had tried these antibodies by different methods and could also submit their own results for entry, possibly with links to Journal articles, much like what some vendors do. It could be a quick reference guide to setting up some research or double staining methods, or just see if some new request is going to fit your routine. Someone would have to have more time and computer skills than I. Thanks all for the input, TOM T > >-----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy >Sent: Friday, March 18, 2011 10:38 AM >To: Histonet >Subject: [Histonet] RE: comprehensive list? > >Tom, > Vendor datasheets are the best source of information. The vast majority of clinical vendors antibodies are designed for formalin fixed paraffin embedded tissue. Research vendors are all over the map and often only test by western blots or elisa so they may or may not have information about how their antibodies work in fixed tissue. > >Tim Morken >Supervisor, Histology, IPOX >UCSF Medical Center >San Francisco, CA, USA > > >-----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom >Sent: Friday, March 18, 2011 9:02 AM >To: Histonet >Subject: [Histonet] comprehensive list? > Hi All, Is there a comprehensive list in one location somewhere that gives information on all or most antibodies on how they work with FFPE or FS or other fixatives, so that we don't have to try to many approaches when we get a new antibody to try. Thanks, Tom T >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From liz <@t> premierlab.com Fri Mar 18 14:14:38 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Mar 18 14:57:09 2011 Subject: [Histonet] RE: comprehensive list? In-Reply-To: Message-ID: Vendor sheets may not be accurate we keep an internal log of all of our antibodies and what species we have tested them in. So far in our lab we have found several antibodies that the vendor states works in a species and it does not in our hands, in these cases we always contact the vendor, basically for a refund and they have updated their specification sheets to reflect the change. We do use protein atlas a lot for verification of the IHC protocols and tissue staining patterns. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of shive003@umn.edu Sent: Friday, March 18, 2011 1:06 PM To: Truscott, Tom Cc: Histonet; Morken,Timothy Subject: Re: [Histonet] RE: comprehensive list? I've also found that occasionally the antigen retrieval method quoted on a vendor's data sheet doesn't unmask antigens as well as another method tried in my initial antibody validation trials with that antibody. So, my point is... any comprehensive list should be used only as a guideline; results in your lab will be affected by the reagents, equipment, and environmental conditions you employ in your own lab. Jan Shivers UMN Vet Diag Lab On Mar 18 2011, Truscott, Tom wrote: Yes, vendor sheets are good, but not all give enough info, and you may have to compare several vendor, and as you say, antibodies only tested by western blot are common in research. Yet, it would be a great money and timesaver if there was one site to go to, to see if anyone had tried these antibodies by different methods and could also submit their own results for entry, possibly with links to Journal articles, much like what some vendors do. It could be a quick reference guide to setting up some research or double staining methods, or just see if some new request is going to fit your routine. Someone would have to have more time and computer skills than I. Thanks all for the input, TOM T > >-----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy >Sent: Friday, March 18, 2011 10:38 AM >To: Histonet >Subject: [Histonet] RE: comprehensive list? > >Tom, > Vendor datasheets are the best source of information. The vast majority of clinical vendors antibodies are designed for formalin fixed paraffin embedded tissue. Research vendors are all over the map and often only test by western blots or elisa so they may or may not have information about how their antibodies work in fixed tissue. > >Tim Morken >Supervisor, Histology, IPOX >UCSF Medical Center >San Francisco, CA, USA > > >-----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom >Sent: Friday, March 18, 2011 9:02 AM >To: Histonet >Subject: [Histonet] comprehensive list? > Hi All, Is there a comprehensive list in one location somewhere that gives information on all or most antibodies on how they work with FFPE or FS or other fixatives, so that we don't have to try to many approaches when we get a new antibody to try. Thanks, Tom T >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From An.Eerdekens <@t> med.kuleuven.be Fri Mar 18 16:00:48 2011 From: An.Eerdekens <@t> med.kuleuven.be (An Eerdekens) Date: Fri Mar 18 16:00:58 2011 Subject: [Histonet] (no subject) Message-ID: <85BD21DE1A6DE94D9A30A2F2C1D2328B3D5805F977@ICTS-S-EXC4-CA.luna.kuleuven.be> Dear, Can you please unsubscribe me from the Histonet list? It was a pleasure to read all the good feedback. Regards An Eerdekens From rjbuesa <@t> yahoo.com Fri Mar 18 16:16:09 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 18 16:16:13 2011 Subject: [Histonet] (no subject) In-Reply-To: <85BD21DE1A6DE94D9A30A2F2C1D2328B3D5805F977@ICTS-S-EXC4-CA.luna.kuleuven.be> Message-ID: <329167.20057.qm@web65706.mail.ac4.yahoo.com> Dear also The only person who can "unsubscribe" you is yourself. Go to the HistoNet website and take care yourself of your request. Ren? J.? --- On Fri, 3/18/11, An Eerdekens wrote: From: An Eerdekens Subject: [Histonet] (no subject) To: "histonet@lists.utsouthwestern.edu" Date: Friday, March 18, 2011, 5:00 PM Dear, Can you please unsubscribe me from the Histonet list? It was a pleasure to read all the good feedback. Regards An Eerdekens _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ael <@t> unc.edu Sat Mar 19 03:43:20 2011 From: ael <@t> unc.edu (Ann Lowder) Date: Sat Mar 19 03:43:25 2011 Subject: [Histonet] Processing animal fat Message-ID: Peggy, I have worked both with animal tissue and human tissue, though never with pig specifically. I do, however, imagine that the pig specimens are extremely fatty much like human breast tissue. The very thick breast specimens that sometimes do not process completely have the same appearance as you have described with the crumbly white areas. I do not know how much time you have to process your specimens or what your workflow is like; however, the really fatty tissue I have seen seems to section better when the processing schedule is longer (but you do have to make sure not to overprocess the tissue either if the skin is still attached as it can harden). The hardest part is finding the medium where you have correctly processed the fat without overdoing the skin. Currently our very fatty breast specimens are processed overnight on a 12 hour process, but I have seen excellent processing even with a 14 hour process if they are very thick specimens (keep in mind this is on an old machine so this could be shortened possibly with something like a Polaris). Enough time in formalin is definitely required as it takes a while for anything to penetrate the fatty cells, but our protocol also ends with extended time in paraffin under vacuum. I think the important part about the paraffin or other stations with the extended processing time means that the processor cycles the reagents more times. It also would depend on if you are microwave processing or using a vacuum processor, but if some came out ok after an hour, maybe including a formalin vacuum station and additional paraffin under vacuum at the end? Are your reagents heated or are you able to include heat while they are processing? We get really excellent feedback from our pathologists about the sections from this process, so I hope some of this info helps. Ann Lowder, HT (ASCP) aelowder@novanthealth.org or ael@unc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Thursday, March 17, 2011 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Processing animal fat My question is directed specifically to veterinary histologists or histologists who also do a fair amount of animal processing. We are having a terrible time processing pig fat. We had problems previously, but thought we had solved them. This latest project (pig skin with a lot of fat attached) came out awful. The fat was not adequately processed: couldn't section it, it just crumbled. In the block, it appears white and crumbly. The funny thing is some blocks came out all right, but most didn't. PLEASE help! Let me know how you process your animal fat (sp. Pig)! Is there a size issue (we trim it if it is greater than 5mm)? We have gotten help from the histonet before and instituted these suggestions (i.e. let sit in formalin for 48 hours; put in cassettes in a 60 degree oven for 1-2 hours and then cool to room temp. Then process using a 1 hour program). Any help would be appreciated. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From gu.lang <@t> gmx.at Sat Mar 19 05:33:26 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Mar 19 05:33:32 2011 Subject: [Histonet] pepsin powder expiration date Message-ID: <6A1B83A932DE414DA4A69C2BB0EE1E65@dielangs.at> Dear histonetters, We have a sigma pepsin powder (7012) in use for our FISH protocol. The container has to be stored at -20?C, but has no labelled expiration date. We have been using the same container for the last 18-20 months. Now we have some troubles with the performance of our FISH-signals. My question is for those, you have experience with this kind of product: How long can such pepsin powders be used without lost of activity? Thanks in advance Gudrun Lang Histolab, Austria From kim.tournear <@t> yahoo.com Sat Mar 19 08:52:43 2011 From: kim.tournear <@t> yahoo.com (Yahoo) Date: Sat Mar 19 08:52:57 2011 Subject: [Histonet] Florida license Message-ID: <96F77B6F-BE53-4A71-B8DB-6C5721B6A02B@yahoo.com> I'm having issues trying to get this license. I was OJT'd many years ago. As a lot of us were. I went through a training consortium from 1992-1994 and I am ASCP certified. I have to have notarized copies (per the app instructions) of my certificates. I contacted a notary public and they said a formal notary document bearing my signature and the person's signature who was a witness to mine, saying that the certificates are true copies, should be attached to my certificates. Can anyone tell me how to get the notary form I need to accompany my certificates? I've already sent an email to the health dept and they were no help to me at all. How did you OJT techs here in Florida get your license! Thanks in advance for all of your help! Sent from the iPhone of Kim Tournear From rjbuesa <@t> yahoo.com Sat Mar 19 09:15:31 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Mar 19 09:15:33 2011 Subject: [Histonet] Florida license In-Reply-To: <96F77B6F-BE53-4A71-B8DB-6C5721B6A02B@yahoo.com> Message-ID: <723209.11499.qm@web65715.mail.ac4.yahoo.com> If you contact any attorney's office they can write a letter of certificate, notarize and that will be enough for your application. Ren? J. --- On Sat, 3/19/11, Yahoo wrote: From: Yahoo Subject: [Histonet] Florida license To: "Histonet" Date: Saturday, March 19, 2011, 9:52 AM I'm having issues trying to get this license. I was OJT'd many years ago. As a lot of us were.? I went through a training consortium from 1992-1994 and I am ASCP certified. I have to have notarized copies (per the app instructions) of my certificates. I contacted a notary public and they said a formal notary document bearing my signature and the person's signature who was a witness to mine, saying that the certificates are true copies, should be attached to my certificates. Can anyone tell me how to get the notary form I need to accompany my certificates? I've already sent an email to the health dept and they were no help to me at all. How did you OJT techs here in Florida get your license! Thanks in advance for all of your help! Sent from the iPhone of Kim Tournear _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kim.tournear <@t> yahoo.com Sat Mar 19 10:36:34 2011 From: kim.tournear <@t> yahoo.com (Yahoo) Date: Sat Mar 19 10:36:52 2011 Subject: [Histonet] Florida license In-Reply-To: <723209.11499.qm@web65715.mail.ac4.yahoo.com> References: <723209.11499.qm@web65715.mail.ac4.yahoo.com> Message-ID: <4AA74406-0760-4166-A3B8-2330213A5776@yahoo.com> Thank you Rene! Sent from the iPhone of Kim Tournear On Mar 19, 2011, at 9:15 AM, Rene J Buesa wrote: > If you contact any attorney's office they can write a letter of certificate, notarize and that will be enough for your application. > Ren? J. > > --- On Sat, 3/19/11, Yahoo wrote: > > From: Yahoo > Subject: [Histonet] Florida license > To: "Histonet" > Date: Saturday, March 19, 2011, 9:52 AM > > I'm having issues trying to get this license. I was OJT'd many years ago. As a lot of us were. I went through a training consortium from 1992-1994 and I am ASCP certified. I have to have notarized copies (per the app instructions) of my certificates. > > I contacted a notary public and they said a formal notary document bearing my signature and the person's signature who was a witness to mine, saying that the certificates are true copies, should be attached to my certificates. > > Can anyone tell me how to get the notary form I need to accompany my certificates? I've already sent an email to the health dept and they were no help to me at all. > > How did you OJT techs here in Florida get your license! > > Thanks in advance for all of your help! > > Sent from the iPhone of Kim Tournear > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From leiker <@t> buffalo.edu Sat Mar 19 22:19:17 2011 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Sat Mar 19 22:19:25 2011 Subject: [Histonet] pepsin powder expiration date Message-ID: <22803.1300591157@buffalo.edu> I'd like to know this one too. I have about a younger supply, a 12-month old bottle of pepsin powder, and will start doing FISH experiments again with it soon. Merced M Leiker Research Technician III Cardiovascular Medicine, 348 BRB State University of New York at Buffalo 3435 Main Street, Buffalo, NY 14214 Ph: (716) 829-6118 On Sat 03/19/11 6:33 AM , "Gudrun Lang" gu.lang@gmx.at sent: > Dear histonetters, > > We have a sigma pepsin powder (7012) in use for our FISH protocol. The > container has to be stored at -20??C, but has no labelled > expiration date. > We have been using the same container for the last 18-20 months. Now we > havesome troubles with the performance of our FISH-signals. > > > > My question is for those, you have experience with this kind of product: > > How long can such pepsin powders be used without lost of activity? > > > > Thanks in advance > > Gudrun Lang > > > > Histolab, Austria > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From trathborne <@t> somerset-healthcare.com Sun Mar 20 10:17:39 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Sun Mar 20 10:18:17 2011 Subject: [Histonet] patients with alias names In-Reply-To: Message-ID: Our facility has a policy about aliases which are allowed only for the specific reasons you stated. After the patient has been discharged, the alias will be merged with the correct patient information, the Pathology department notified, and an addendum added to the report if it has already been signed out. Slide labels are added to the slide, preferably on the front, but on the back of the slide if the entire slide shows specimen. This way, both names can be referenced. If we are made aware of the change before the case is signed out, a note is still dictated into the report because you still have to explain the discrepancy of the name on the requisition and container. It is very important to communicate with Registration in the case of consultations. You can't have a reference lab bill a Jane Doe, so the merge will need to occur before the specimen is sent out (or at least before you are billed). Hope this helps. We had some similar concerns last year, which now seem to be resolved. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of mwhite@mcleodhealth.org Sent: Friday, March 18, 2011 11:19 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] patients with alias names Histonetters: We are curious to find out how your facilities handle patients with alias names. At our hospital, patients are somewhat frequently admitted with alias names in order to protect their privacy and/or safety (i.e., gunshot wounds, gang crime, etc.) . Usually their pathology is done without us ever knowing that there was an alias. So, we have slides and blocks that are labeled with an incorrect name along with the case number, etc. If the patient was in our system previously, they use their existing permanent medical record number. Upon discharge from our hospital computer system, these patients' medical records are merged or corrected somehow with the real name , and the name is updated in our pathology LIS and our clinical lab LIS. Occasionally the discharge is timed in such a way that the path report hasn't yet been signed out; therefore the slides, blocks, gross description, etc. all contain an alias name that appears as a discrepancy to the pathologist ( who sees the discharged patient's real name on the header of the typed gross paperwork.) Our pathologists are very uncomfortable with the slides and blocks having the alias name. What if they want to send out these materials to other facilities for consults? We don't get any report that shows a summary of aliases. What are your policies and procedures? Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmaster@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From kdwyer3322 <@t> aol.com Sun Mar 20 10:40:14 2011 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sun Mar 20 10:40:39 2011 Subject: [Histonet] TEXAS SOCIETY FOR HISTOTECHNOLOGY 2011 Symposium/Convention Message-ID: <8CDB52C3DB13C8F-191C-2715D@webmail-m039.sysops.aol.com> All, The TSH meeting is only a 12 days away and you still have time to sign up for one or more of 15 workshops and symposiums, view 40 vendors and meet and connect with your fellow histotechs. If need need more information please contact me through this e-mail. Regards, Kathy Dwyer TSH Convention Coordinator From histotalk <@t> yahoo.com Sun Mar 20 17:21:08 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Sun Mar 20 17:21:12 2011 Subject: [Histonet] Re: TEXAS SOCIETY FOR HISTOTECHNOLOGY 2011 Symposium / Convention Message-ID: <137523.14478.qm@web120615.mail.ne1.yahoo.com> Hi Kathy - I announced the TSH 2011 Sym/Con on my last Feb show and my first March show. I hope that it was able to help. Yours, David www.HistoTALK.com From jsantiago <@t> bellsouth.net Mon Mar 21 06:36:34 2011 From: jsantiago <@t> bellsouth.net (Jerry Santiago) Date: Mon Mar 21 06:36:39 2011 Subject: [Histonet] Florida Society for Histotechnology Annual Meeting Message-ID: <861203.7078.qm@web180802.mail.gq1.yahoo.com> Hello Histonetters, The Florida Society for Histotechnology announces their annual meeting to be held at the Grand Hyatt Tampa Bay in Tampa, Florida from May 19-22, 2011. Meeting information is available for exhibitors and attendees by visiting our website at www.fshgroup.org, then 2011 Meeting. This years community benefit event will be for the Boys & Girls Club of Florida. Thank you everyone for your contnued support. For more information please contact Susan Clark at 954-265-5371 or Jerry Santiago at 904-505-9989. From PMonfils <@t> Lifespan.org Mon Mar 21 09:21:56 2011 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Mar 21 09:22:11 2011 Subject: [Histonet] Procedure for making Gram Control In-Reply-To: References: Message-ID: <4EBFF65383B74D49995298C4976D1D5E07E7971A@LSRIEXCH1.lsmaster.lifespan.org> I have made my own gram controls in the past. I purchase liquid bacterial cultures of gram+ and gram- organisms from a biological supply company (http://www.ctvalleybio.com), spin them down to concentrate the organisms, then inject the concentrated cultures into a fresh piece of lung, gram+ at one end of the tissue, gram- at the other end, or separate pieces for gram+ and gram-. I use lung because there is plenty of empty space to inject into. If you use something like liver, there is no place for the injection to go except by forcing the tissue apart. I put the needle in to about the midpoint of the tissue, then withdraw it slowly as I inject, to distribute the culture through the tissue. Then just drop the tissue into formalin and process as usual. The company has quite a variety of organisms for less than $10 per culture, so you can choose bacilli vs. cocci in various configurations. If you really want to get fancy you can have gram+ bacilli and gram+ cocci at one end of the tissue, gram- bacilli and gram- cocci at the other end. From JMacDonald <@t> mtsac.edu Mon Mar 21 10:23:11 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Mar 21 10:23:17 2011 Subject: [Histonet] California Symposium Message-ID: The program is ready for the CSH scheduled for May 13-15 in Concord, California. For more information see the link below. http://www.californiahistology.org/csh2011/index.html From MadaryJ <@t> MedImmune.com Mon Mar 21 10:26:38 2011 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Mon Mar 21 10:26:46 2011 Subject: [Histonet] PFA preparation Message-ID: <29A3CB81288E6F4BA2C9B3C8015A9A130382908C@MD1EV002.medimmune.com> Anyone have a decent method to prepare paraformaldehyde. I have the powder and the NOAH, hood, heat source but forgot the method. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From Allison_Scott <@t> hchd.tmc.edu Mon Mar 21 10:56:07 2011 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Mon Mar 21 10:56:09 2011 Subject: [Histonet] FW: Slide labeling procedure Message-ID: <1872B4A455B7974391609AD8034C79FC026DFD96@LBEXCH01.hchd.local> > ______________________________________________ > From: Scott, Allison D > Sent: Monday, March 21, 2011 10:55 AM > To: 'histonet@list.utsouthwestern.edu' > Subject: Slide labeling procedure > > Hello to all in histoland. The CAP inspectors are here. Does anyone > have a slide labeling procedure that they would be willing to share. > Any help would be appreciated. > > > Allison Scott CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From MSHERWOOD <@t> PARTNERS.ORG Mon Mar 21 11:01:40 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Mar 21 11:01:44 2011 Subject: [Histonet] PFA preparation In-Reply-To: <29A3CB81288E6F4BA2C9B3C8015A9A130382908C@MD1EV002.medimmune.com> References: <29A3CB81288E6F4BA2C9B3C8015A9A130382908C@MD1EV002.medimmune.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB55A4@PHSXMB30.partners.org> I stopped making up paraformaldehyde from the powder; too toxic. Another colleague told me she buys it in a 16% solution, EM grade from EMS (10 x 10ml vials). I started using that, along with the glutaraldehyde to prepare my working solution. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph Sent: Monday, March 21, 2011 11:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PFA preparation Anyone have a decent method to prepare paraformaldehyde. I have the powder and the NOAH, hood, heat source but forgot the method. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Montina.VanMeter <@t> pbrc.edu Mon Mar 21 11:25:09 2011 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Mon Mar 21 11:25:12 2011 Subject: [Histonet] PFA preparation In-Reply-To: <29A3CB81288E6F4BA2C9B3C8015A9A130382908C@MD1EV002.medimmune.com> References: <29A3CB81288E6F4BA2C9B3C8015A9A130382908C@MD1EV002.medimmune.com> Message-ID: <4FE7FB862E90E448AE32388E759220E502ACE464@pbrcas31.pbrc.edu> Nick, I too have tried to limit my exposure after making it from scratch for 30+ years. I currently purchase it from USB Corp. located in Cleveland Ohio. They have been acquired by Affymetrix and you will be directed to that website when you type in www.usbweb.com. Cat.# 19943 1LT Paraformaldehyde Solution, 4% in PBS, 1L $48.00 Regards, Tina Montina J. Van Meter Lab Manager Autonomic Neuroscience 225-763-2564 vanmetmj@pbrc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph Sent: Monday, March 21, 2011 10:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PFA preparation Anyone have a decent method to prepare paraformaldehyde. I have the powder and the NOAH, hood, heat source but forgot the method. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From member <@t> linkedin.com Mon Mar 21 11:27:18 2011 From: member <@t> linkedin.com (Dana Dittus via LinkedIn) Date: Mon Mar 21 11:27:23 2011 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <60778266.5835465.1300724838414.JavaMail.app@ela4-bed40.prod> LinkedIn ------------Dana Dittus requested to add you as a connection on LinkedIn: ------------------------------------------ Jackie, I'd like to add you to my professional network on LinkedIn. - Dana Accept invitation from Dana Dittus http://www.linkedin.com/e/-gzuoej-gljlzgnc-60/XlF1SInfsMz-GtKrGcmJOZZO1lkFwT3RZom15InljeuxcwwTts/blk/I1210086835_3/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPnPkPe3oUc30Ncz59bS4VpztHdmEMbPoUczwTcjgQdj8LrCBxbOYWrSlI/EML_comm_afe/ View invitation from Dana Dittus http://www.linkedin.com/e/-gzuoej-gljlzgnc-60/XlF1SInfsMz-GtKrGcmJOZZO1lkFwT3RZom15InljeuxcwwTts/blk/I1210086835_3/3dvdjcUdzwMc34OckALqnpPbOYWrSlI/svi/ ------------------------------------------ DID YOU KNOW LinkedIn can help you find the right service providers using recommendations from your trusted network? Using LinkedIn Services, you can take the risky guesswork out of selecting service providers by reading the recommendations of credible, trustworthy members of your network. http://www.linkedin.com/e/-gzuoej-gljlzgnc-60/svp/inv-25/ -- (c) 2011, LinkedIn Corporation From pruegg <@t> ihctech.net Mon Mar 21 11:44:04 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Mar 21 11:43:58 2011 Subject: [Histonet] RE: comprehensive list? In-Reply-To: References: Message-ID: I find the histonet and the IHCRG list servers the best sources for what has been tried and what the results are. If you do not already participate in the IHC Resource Group (this has just an IHC focus and is not nearly as busy with all histology as Histonet is, we do not even get messages posted everyday, but I find those on the IHCRG list server to be great experts in IHC) to join the IHC Resource Group list server go to www.ihcrg.org and register as a member (NSH membership is required but if you are not a member of NSH they will show you how to become one). I usually do not try a new ab without asking the IHCRG list server members what their experiences may have been with that antibody. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Friday, March 18, 2011 1:15 PM To: shive003@umn.edu; Truscott, Tom Cc: Histonet; Morken,Timothy Subject: RE: [Histonet] RE: comprehensive list? Vendor sheets may not be accurate we keep an internal log of all of our antibodies and what species we have tested them in. So far in our lab we have found several antibodies that the vendor states works in a species and it does not in our hands, in these cases we always contact the vendor, basically for a refund and they have updated their specification sheets to reflect the change. We do use protein atlas a lot for verification of the IHC protocols and tissue staining patterns. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of shive003@umn.edu Sent: Friday, March 18, 2011 1:06 PM To: Truscott, Tom Cc: Histonet; Morken,Timothy Subject: Re: [Histonet] RE: comprehensive list? I've also found that occasionally the antigen retrieval method quoted on a vendor's data sheet doesn't unmask antigens as well as another method tried in my initial antibody validation trials with that antibody. So, my point is... any comprehensive list should be used only as a guideline; results in your lab will be affected by the reagents, equipment, and environmental conditions you employ in your own lab. Jan Shivers UMN Vet Diag Lab On Mar 18 2011, Truscott, Tom wrote: Yes, vendor sheets are good, but not all give enough info, and you may have to compare several vendor, and as you say, antibodies only tested by western blot are common in research. Yet, it would be a great money and timesaver if there was one site to go to, to see if anyone had tried these antibodies by different methods and could also submit their own results for entry, possibly with links to Journal articles, much like what some vendors do. It could be a quick reference guide to setting up some research or double staining methods, or just see if some new request is going to fit your routine. Someone would have to have more time and computer skills than I. Thanks all for the input, TOM T > >-----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy >Sent: Friday, March 18, 2011 10:38 AM >To: Histonet >Subject: [Histonet] RE: comprehensive list? > >Tom, > Vendor datasheets are the best source of information. The vast majority of clinical vendors antibodies are designed for formalin fixed paraffin embedded tissue. Research vendors are all over the map and often only test by western blots or elisa so they may or may not have information about how their antibodies work in fixed tissue. > >Tim Morken >Supervisor, Histology, IPOX >UCSF Medical Center >San Francisco, CA, USA > > >-----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom >Sent: Friday, March 18, 2011 9:02 AM >To: Histonet >Subject: [Histonet] comprehensive list? > Hi All, Is there a comprehensive list in one location somewhere that gives information on all or most antibodies on how they work with FFPE or FS or other fixatives, so that we don't have to try to many approaches when we get a new antibody to try. Thanks, Tom T >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Mon Mar 21 11:43:43 2011 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Mon Mar 21 11:44:28 2011 Subject: [Histonet] PFA preparation In-Reply-To: <4FE7FB862E90E448AE32388E759220E502ACE464@pbrcas31.pbrc.edu> References: <29A3CB81288E6F4BA2C9B3C8015A9A130382908C@MD1EV002.medimmune.com> <4FE7FB862E90E448AE32388E759220E502ACE464@pbrcas31.pbrc.edu> Message-ID: <6781F227A74F692302B73510@CDYwxp1931.ad.med.buffalo.edu> I concur with the others. For about 6 years I've made it from powder but the toxicity risk bothered me until I realized how safely and cheaply you can just buy the same stuff. We get a discount through our stockroom on a 4L bottle of 10% Buffered Formalin from Fisher (SF100-4) for $28. I started getting neutral buffered formalin instead from Fisher. --On Monday, March 21, 2011 11:25 AM -0500 Montina Van Meter wrote: > Nick, > > I too have tried to limit my exposure after making it from scratch for > 30+ years. I currently purchase it from USB Corp. located in Cleveland > Ohio. They have been acquired by Affymetrix and you will be directed to > that website when you type in www.usbweb.com. > > Cat.# 19943 1LT Paraformaldehyde Solution, 4% in PBS, 1L $48.00 > > > Regards, > Tina > > > > > > Montina J. Van Meter > Lab Manager > Autonomic Neuroscience > 225-763-2564 > vanmetmj@pbrc.edu > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, > Joseph > Sent: Monday, March 21, 2011 10:27 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] PFA preparation > > Anyone have a decent method to prepare paraformaldehyde. I have the > powder and the NOAH, hood, heat source but forgot the method. > > > Nick Madary, HT/HTL(ASCP)QIHC > > Histology Mgr, Medimmune > > 301.398.6360(lab), 4745(vm),9745(fax) > > > "To the extent this electronic communication or any of its attachments > contain information that is not in the public domain, such information > is considered by MedImmune to be confidential and proprietary, and > expected to be used only by the individual(s) for whom it is intended. > If you have received this electronic communication in error, please > reply to the sender advising of the error in transmission and delete the > original message and any accompanying documents from your system > immediately, without copying, reviewing or otherwise using them for any > purpose. Thank you for your cooperation." > > > To the extent this electronic communication or any of its attachments > contain information that is not in the public domain, such information > is considered by MedImmune to be confidential and proprietary. This > communication is expected to be read and/or used only by the > individual(s) for whom it is intended. If you have received this > electronic communication in error, please reply to the sender advising > of the error in transmission and delete the original message and any > accompanying documents from your system immediately, without copying, > reviewing or otherwise using them for any purpose. Thank you for your > cooperation. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From pruegg <@t> ihctech.net Mon Mar 21 11:48:26 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Mar 21 11:48:19 2011 Subject: [Histonet] comprehensive list? In-Reply-To: <535303.82446.qm@web65701.mail.ac4.yahoo.com> References: <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB3D@CVMMBX.vetmed.wsu.edu> <535303.82446.qm@web65701.mail.ac4.yahoo.com> Message-ID: Novacastra had a cross reactivity list for their abs which I find very helpful, I have the old list but can't seem to find anyone who knows about it recently, I got it years ago when the instrument was still with the AU Co. Vision BioSystems, I will ask Leica reps to see if they know about this list. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, March 18, 2011 11:30 AM To: Histonet; TomTruscott Subject: Re: [Histonet] comprehensive list? Try your DAKO representative. NOVOCASTRA also used to have such a list for their Abs. Ren? J. --- On Fri, 3/18/11, Truscott, Tom wrote: From: Truscott, Tom Subject: [Histonet] comprehensive list? To: "Histonet" Date: Friday, March 18, 2011, 12:02 PM Hi All, Is there a comprehensive list in one location somewhere that gives information on all or most antibodies on how they work with FFPE or FS or other fixatives, so that we don't have to try to many approaches when we get a new antibody to try. Thanks, Tom T _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Mon Mar 21 11:55:02 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Mon Mar 21 11:55:12 2011 Subject: [Histonet] PFA preparation In-Reply-To: <6781F227A74F692302B73510@CDYwxp1931.ad.med.buffalo.edu> References: <29A3CB81288E6F4BA2C9B3C8015A9A130382908C@MD1EV002.medimmune.com><4FE7FB862E90E448AE32388E759220E502ACE464@pbrcas31.pbrc.edu> <6781F227A74F692302B73510@CDYwxp1931.ad.med.buffalo.edu> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB55A6@PHSXMB30.partners.org> We also purchase a 4L cubitainer of 10% buffered formalin from Fisher. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Monday, March 21, 2011 12:44 PM To: Montina Van Meter; Madary, Joseph; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PFA preparation I concur with the others. For about 6 years I've made it from powder but the toxicity risk bothered me until I realized how safely and cheaply you can just buy the same stuff. We get a discount through our stockroom on a 4L bottle of 10% Buffered Formalin from Fisher (SF100-4) for $28. I started getting neutral buffered formalin instead from Fisher. --On Monday, March 21, 2011 11:25 AM -0500 Montina Van Meter wrote: > Nick, > > I too have tried to limit my exposure after making it from scratch for > 30+ years. I currently purchase it from USB Corp. located in Cleveland > Ohio. They have been acquired by Affymetrix and you will be directed to > that website when you type in www.usbweb.com. > > Cat.# 19943 1LT Paraformaldehyde Solution, 4% in PBS, 1L $48.00 > > > Regards, > Tina > > > > > > Montina J. Van Meter > Lab Manager > Autonomic Neuroscience > 225-763-2564 > vanmetmj@pbrc.edu > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, > Joseph > Sent: Monday, March 21, 2011 10:27 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] PFA preparation > > Anyone have a decent method to prepare paraformaldehyde. I have the > powder and the NOAH, hood, heat source but forgot the method. > > > Nick Madary, HT/HTL(ASCP)QIHC > > Histology Mgr, Medimmune > > 301.398.6360(lab), 4745(vm),9745(fax) > > > "To the extent this electronic communication or any of its attachments > contain information that is not in the public domain, such information > is considered by MedImmune to be confidential and proprietary, and > expected to be used only by the individual(s) for whom it is intended. > If you have received this electronic communication in error, please > reply to the sender advising of the error in transmission and delete the > original message and any accompanying documents from your system > immediately, without copying, reviewing or otherwise using them for any > purpose. Thank you for your cooperation." > > > To the extent this electronic communication or any of its attachments > contain information that is not in the public domain, such information > is considered by MedImmune to be confidential and proprietary. This > communication is expected to be read and/or used only by the > individual(s) for whom it is intended. If you have received this > electronic communication in error, please reply to the sender advising > of the error in transmission and delete the original message and any > accompanying documents from your system immediately, without copying, > reviewing or otherwise using them for any purpose. Thank you for your > cooperation. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From kimd <@t> slonepartners.com Mon Mar 21 12:26:40 2011 From: kimd <@t> slonepartners.com (Kim Wilson) Date: Mon Mar 21 12:26:44 2011 Subject: [Histonet] Seeking a Histotechnologist in beautiful Washington DC Message-ID: <20110321172731.649CC5337A@qmail2.topechelon.com> Slone Partners seeks laboratory, based in a ASCP certification experience in a high-volume laboratory. Special features of the position: curiosity and enjoys mentoring and developing Interested and qualified candidates should Kim Wilson at [1]kimd@slonepartners.com. KIM WILSON - EXECUTIVE FORT WORTH, TEXAS CORPORATE HEADQUARTERS 1521 ALTON TOLL DIRECT: FAX: 866-297-8848[2] KIMD@SLONEPARTNERS.COM SLONEPARTNERS.COM The information contained information owned by Slone Partners and entry named above. If you receive this in not transmit further. References 1. 3D"mailto:kimd@slonepartners.com" 2. 3D"mailto:KIMD@SLONEPARTNERS.COM" From darcyb <@t> slonepartners.com Mon Mar 21 12:28:05 2011 From: darcyb <@t> slonepartners.com (Darcy Bloch) Date: Mon Mar 21 12:28:08 2011 Subject: [Histonet] Seeking Histotechnologists in upstate New York Message-ID: <20110321172856.780C75331B@qmail2.topechelon.com> Slone Located is looking for dynamic team. If you are dedicated to patient care, and that values those qualities, this looking for. The ideal science or higher and will also consider experienced transitioning to histology. All candidates must license or be eligible for NY licensure. Special features about this position: significant opportunities for advancement and has outstanding benefits. If for this [1]darcyb@slonepartners.com. SLONEPARTNERS DARCY BLOCH - ASSOCIATE DIRECTOR OF RESEARCH NORFOLK, CORPORATE 1521 ALTON ROAD, #638 MIAMI BEACH, FL 33139 TOLL FREE: 866-870-7434 FAX: 866-297-8848 [2]DARCYB@SLONEPARTNERS.COM SLONEPARTNERS.COM The information contained is privileged and confidential information owned by Slone Partners and is only for the individual or entry not transmit References 1. 3D"mailto:darcyb@slonepartners.com" 2. 3D"mailto:DARCYB@SLONEPARTNERS.COM" From jaylundgren <@t> gmail.com Mon Mar 21 13:01:03 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Mar 21 13:01:08 2011 Subject: [Histonet] cell block agar In-Reply-To: <404613.90131.qm@web120718.mail.ne1.yahoo.com> References: <404613.90131.qm@web120718.mail.ne1.yahoo.com> Message-ID: If one is in a hospital setting, I just go into Micro and get a TSA slant. Loosen the top and pop it in the microwave for 5-10 seconds. Ta-daa! Cell block! Sincerely, Jay A. Lundgren M.S., HTL (ASCP) From jaylundgren <@t> gmail.com Mon Mar 21 13:07:12 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Mar 21 13:07:17 2011 Subject: [Histonet] H-PAS and Modified Alcian Blue In-Reply-To: <5680DA93771F0C48954CC8D38425E72401AB351D@ISMAIL.parrishmed.local> References: <5680DA93771F0C48954CC8D38425E72401AB351D@ISMAIL.parrishmed.local> Message-ID: Never heard of H-PAS, but a PASH is Periodic Acid - Schiff's with Hematoxylin. A PAS with a hematoxylin counterstain (NO eosin). Sincerely, Jay A. Lundgren M.S., HTL (ASCP) From wbenton <@t> cua.md Mon Mar 21 13:14:59 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Mon Mar 21 13:15:50 2011 Subject: [Histonet] H-PAS and Modified Alcian Blue In-Reply-To: References: <5680DA93771F0C48954CC8D38425E72401AB351D@ISMAIL.parrishmed.local>, Message-ID: <0B8979A204680A42B93A52B486088CD91D6EC7CBAC@CUAEXH1.GCU-MD.local> Probably a hyaluronidase PAS. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren [jaylundgren@gmail.com] Sent: Monday, March 21, 2011 2:07 PM To: Hannen, Valerie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] H-PAS and Modified Alcian Blue Never heard of H-PAS, but a PASH is Periodic Acid - Schiff's with Hematoxylin. A PAS with a hematoxylin counterstain (NO eosin). Sincerely, Jay A. Lundgren M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From smcbride <@t> andrew.cmu.edu Mon Mar 21 13:23:40 2011 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Mon Mar 21 13:23:46 2011 Subject: [Histonet] Grossing Station Recommendation Message-ID: <13EE4E74-52D4-4909-B190-B1CBC1FD3296@andrew.cmu.edu> Colleagues, We are looking to replace our current pathology grossing hood with a new station, so I am looking for recommendations. Thanks in advance for all of your wonderful advice. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu From HornHV <@t> archildrens.org Mon Mar 21 13:25:37 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Mar 21 13:25:43 2011 Subject: [Histonet] Grossing Station Recommendation In-Reply-To: <13EE4E74-52D4-4909-B190-B1CBC1FD3296@andrew.cmu.edu> References: <13EE4E74-52D4-4909-B190-B1CBC1FD3296@andrew.cmu.edu> Message-ID: <25A4DE08332B19499904459F00AAACB7194516A780@EVS1.archildrens.org> We love our Gross Lab Sr. ThermoShandon Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sean McBride Sent: Monday, March 21, 2011 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Station Recommendation Colleagues, We are looking to replace our current pathology grossing hood with a new station, so I am looking for recommendations. Thanks in advance for all of your wonderful advice. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From POWELL_SA <@t> mercer.edu Mon Mar 21 13:25:38 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Mar 21 13:25:46 2011 Subject: [Histonet] Grossing Station Recommendation In-Reply-To: <13EE4E74-52D4-4909-B190-B1CBC1FD3296@andrew.cmu.edu> References: <13EE4E74-52D4-4909-B190-B1CBC1FD3296@andrew.cmu.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE238D4CDE9F8@MERCERMAIL.MercerU.local> Try Mopec. http://www.mopec.com/category/5/grossing_stations__workstations/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sean McBride Sent: Monday, March 21, 2011 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Station Recommendation Colleagues, We are looking to replace our current pathology grossing hood with a new station, so I am looking for recommendations. Thanks in advance for all of your wonderful advice. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Mon Mar 21 13:28:54 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Mar 21 13:28:58 2011 Subject: [Histonet] Grossing Station Recommendation In-Reply-To: <13EE4E74-52D4-4909-B190-B1CBC1FD3296@andrew.cmu.edu> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974B8C@is-e2k3.grhs.net> Gross lab senior -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sean McBride Sent: Monday, March 21, 2011 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Station Recommendation Colleagues, We are looking to replace our current pathology grossing hood with a new station, so I am looking for recommendations. Thanks in advance for all of your wonderful advice. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abeharry798 <@t> gmail.com Mon Mar 21 15:00:41 2011 From: abeharry798 <@t> gmail.com (Gmail) Date: Mon Mar 21 15:00:59 2011 Subject: [Histonet] Grossing Station Recommendation In-Reply-To: <9BF995BC0E47744E9673A41486E24EE238D4CDE9F8@MERCERMAIL.MercerU.local> References: <13EE4E74-52D4-4909-B190-B1CBC1FD3296@andrew.cmu.edu> <9BF995BC0E47744E9673A41486E24EE238D4CDE9F8@MERCERMAIL.MercerU.local> Message-ID: We also use the stations from mopec Sent from Lawrence's iPhone On 2011-03-21, at 2:25 PM, "Shirley A. Powell" wrote: > Try Mopec. > > http://www.mopec.com/category/5/grossing_stations__workstations/ > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Sean McBride > Sent: Monday, March 21, 2011 2:24 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Grossing Station Recommendation > > Colleagues, > > We are looking to replace our current pathology grossing hood with a > new station, so I am looking for recommendations. Thanks in advance > for all of your wonderful advice. > > > Best regards, > > > ~Sean McBride > > > Scientific Specialist > Bone Tissue Engineering Center > Carnegie Mellon Research Institute > Suite 4311 > 700 Technology Drive > Pittsburgh, PA 15219-3124 > > 412-268-8275 (o) > 412-915-1683 (m) > 412-268-8275 (fax) > smcbride@andrew.cmu.edu > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Mon Mar 21 15:44:58 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Mon Mar 21 15:45:19 2011 Subject: [Histonet] PRN in Longmont Colorado Message-ID: <4D87646A020000A800058BA0@ns.luhcares.org> Hey all. We have a PRN position open for an HT here at Longmont United Hospital. Go to luhcares.org to check it out. Thanks Matt Lunetta, HT (ASCP) From Jessica <@t> nsh.org Mon Mar 21 15:46:41 2011 From: Jessica <@t> nsh.org (Jessica Smith) Date: Mon Mar 21 15:46:50 2011 Subject: [Histonet] NSH One-Day VIR Forum Message-ID: The NSH One Day Forum in Veterinary, Research & Industry Histology is coming up fast so don't forget to register! The event takes place Saturday, March 26, 2011, 8:30 am - 4:15 pm in Raleigh, NC. Check out the great program below for a one time low fee which includes continental breakfast, lunch and refreshment breaks! Sessions Include: * Digital Pathology - A New Era for Pathology, Presented by Curtis Adams, PhD, Sr. Product Manager, Life Sciences, Aperio * Developing, Trouble Shooting, and Optimizing IHC Detection Systems for Non-Human Tissues, Presented by Elizabeth Chlipala, BS, HTL(ASCP)QIHC, Manager, Premier Laboratory, LLC * Laser Microdissection: The Before and After, Presented by Diane L. Sterchi, MS, HT(ASCP)HTL, Sr. Research Associate, Covance, Inc. * Tissue Grossing & Pathology: Descriptive Methods & Terminology, Presented by Richard French, DVM, PhD, Senior Veterinary Pathologsit & Clinical Professor, University of New Hampshire Visit our Event Page to find out more information! Register Online Today! Or fax in the VIR Brochure to 443.535.4055. From POWELL_SA <@t> mercer.edu Mon Mar 21 16:05:15 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Mar 21 16:05:20 2011 Subject: [Histonet] GSH meeting reminder Message-ID: <9BF995BC0E47744E9673A41486E24EE238D4CDECB9@MERCERMAIL.MercerU.local> Hi Everyone, this is the last time I promise this year, but the GSH meeting is coming up this weekend. You can still register for the workshops/lectures, and the HT/HTL review on Friday at 1, awards luncheon on Saturday and the general membership meeting, vendor exhibits reception on Friday night, and exhibits all day Saturday. Come and connect with old friends. We are expecting a wonderful meeting, wonderful weather and hope you can join us. I have attached a copy of the program for those of you who were uncertain that you could make it. Come to Callaway Gardens at Pine Mountain, Georgia for a great meeting. You can find more information at www.histosearch.com/gsh and click on symposium tab at the bottom. Vendor registration forms can be downloaded from there as well. Shirley Powell GSH Secretary Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From Allison_Scott <@t> hchd.tmc.edu Mon Mar 21 19:03:27 2011 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Mon Mar 21 19:03:32 2011 Subject: [Histonet] CAP Inspection Message-ID: <1872B4A455B7974391609AD8034C79FC019C19EA@LBEXCH01.hchd.local> Hello to all in histoland. I would like thank everyone that helped me out with procedures. I really appreciate it. Our CAP was today. We had no deficiencies. Now I can have my life back. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From sabeti_shahram <@t> yahoo.com Tue Mar 22 03:40:08 2011 From: sabeti_shahram <@t> yahoo.com (Shahram Sabeti) Date: Tue Mar 22 03:40:11 2011 Subject: [Histonet] how to bleach melanoma cases without bad effect on IHC Message-ID: <457061.45898.qm@web35604.mail.mud.yahoo.com> hello dear fellows, ?? i have an IHC project on paraffin embedded melanoma cases; some of them being heavily pigmented. i have seen many protocols based on H2O2 to bleach the pigmented ones, but i am not sure about any possible effect on antigen retrieval.please guide me . yours, sabeti From TMcNemar <@t> lmhealth.org Tue Mar 22 05:10:58 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Mar 22 05:11:01 2011 Subject: [Histonet] Grossing Station Recommendation In-Reply-To: <13EE4E74-52D4-4909-B190-B1CBC1FD3296@andrew.cmu.edu> References: <13EE4E74-52D4-4909-B190-B1CBC1FD3296@andrew.cmu.edu> Message-ID: Grosslab Senior here as well. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sean McBride Sent: Monday, March 21, 2011 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Station Recommendation Colleagues, We are looking to replace our current pathology grossing hood with a new station, so I am looking for recommendations. Thanks in advance for all of your wonderful advice. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Melissa.Kuhnla <@t> chsli.org Tue Mar 22 05:17:51 2011 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Tue Mar 22 05:17:57 2011 Subject: [Histonet] antibody instensity Message-ID: Hi All, My management team just asked me how feasible a 24 hours IHC lab would be. Are any of you currently running 24 hours? What is the average turn around time for your IHC? We are currently using Ventana platforms and mainly Ventana and Cell Marque antibodies. I do have concern with keeping most of the antibodies at room temp all day, every day. Has anyone experienced a drift in intensity with any antibodies? Any input for or against would be appreciated. Thank you. :-) Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From LSebree <@t> uwhealth.org Tue Mar 22 07:49:59 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Mar 22 07:50:03 2011 Subject: [Histonet] how to bleach melanoma cases without bad effect on IHC In-Reply-To: <457061.45898.qm@web35604.mail.mud.yahoo.com> References: <457061.45898.qm@web35604.mail.mud.yahoo.com> Message-ID: <8C023B4AB999614BA4791BAEB26E2738010029D3@UWHC-MAIL01.uwhis.hosp.wisc.edu> Sabeti, If you have the option of using a detection system with a chromogen other than DAB, i.e. Alkaline Phosphatase Red, that is by far the easiest way to get around the pigment issue. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shahram Sabeti Sent: Tuesday, March 22, 2011 3:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how to bleach melanoma cases without bad effect on IHC hello dear fellows, ?? i have an IHC project on paraffin embedded melanoma cases; some of them being heavily pigmented. i have seen many protocols based on H2O2 to bleach the pigmented ones, but i am not sure about any possible effect on antigen retrieval.please guide me . yours, sabeti _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kaylee <@t> medwrench.com Tue Mar 22 07:52:43 2011 From: kaylee <@t> medwrench.com (Kaylee McCaffrey) Date: Tue Mar 22 07:52:51 2011 Subject: [Histonet] Beckman Coulter HmX-Running the Analyzer without DMS Message-ID: <6DFE06B0-75B3-4CD5-BD24-AF3A439E5E24@medwrench.com> Our computer went down and we are waiting for service. Is there a way we can run the HmX? In the past before computers all analyzers were able to work just fine and print the results. Kaylee McCaffrey MedWrench Product Manager From jo-ann.bader <@t> mcgill.ca Tue Mar 22 08:24:35 2011 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Tue Mar 22 08:27:46 2011 Subject: [Histonet] Heck Message-ID: <76D119EF12C904418800ED67CCB2062929A1F1E650@EXMBXVS1.campus.mcgill.ca> Hi Helen, Can you check the blocks I sent you and see if you have 3 blocks marked EXP 0012. We have so many back up systems in place that it is almost as if I never received these blocks but I have to check anyway. Thanks Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 (3355) Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 From Saro.Bascaramurty <@t> nrc-cnrc.gc.ca Tue Mar 22 08:59:58 2011 From: Saro.Bascaramurty <@t> nrc-cnrc.gc.ca (Bascaramurty, Saro) Date: Tue Mar 22 09:00:04 2011 Subject: [Histonet] Frozen sectioning of pig skin Message-ID: I have sectioned some formalin fixed, paraffin embedded pig skin blocks in the past, but never the fresh frozen pig skin blocks. The samples were frozen by the researcher in liquid nitrogen, but not covered in cryomatrix and have been stored in the deep freezer for over a year. I have now embedded those blocks in cryomatrix and wanted to prepare sections at 30um and 6-8um thickness. When I tried it, I had problems getting good quality sections in spite of using brand new blades. I tried lowering the temperature from -18 deg.C to -22 / -25 deg.C. I still couldn't get quality sections consistently. If anyone from histoland has some good tips to improve the quality of these sections, I would greatly appreciate it. Thank you, Saro Bascaramurty Technical Officer Institute for Biodiagnostics National Research Council 435 Ellice Avenue, Winnipeg, Manitoba. R3B 1Y6 Tel: 204-984-7166 Fax:204-984-6978 email:saro.bascaramurty@nrc-cnrc.gc.ca From cpyse <@t> x-celllab.com Tue Mar 22 09:01:30 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Mar 22 09:01:42 2011 Subject: [Histonet] how to bleach melanoma cases without bad effect on IHC In-Reply-To: <457061.45898.qm@web35604.mail.mud.yahoo.com> References: <457061.45898.qm@web35604.mail.mud.yahoo.com> Message-ID: <000f01cbe899$a4fa79b0$eeef6d10$@com> Sabeti I use the AEC chromogen (Dako catalog #K346989) for my melanoma cases. I use a aqueous mounting media, placed on the tissue only, dry it completely on a hot plate. When dry I coverslip it with permanent mounting media(don't place it in xylene), dry coverslip. Works great, and the color doesn't fade. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shahram Sabeti Sent: Tuesday, March 22, 2011 4:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how to bleach melanoma cases without bad effect on IHC hello dear fellows, ?? i have an IHC project on paraffin embedded melanoma cases; some of them being heavily pigmented. i have seen many protocols based on H2O2 to bleach the pigmented ones, but i am not sure about any possible effect on antigen retrieval.please guide me . yours, sabeti _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Saro.Bascaramurty <@t> nrc-cnrc.gc.ca Tue Mar 22 09:19:21 2011 From: Saro.Bascaramurty <@t> nrc-cnrc.gc.ca (Bascaramurty, Saro) Date: Tue Mar 22 09:19:26 2011 Subject: [Histonet] Frozen sectioning of pig skin In-Reply-To: References: Message-ID: No, I did not. We are using Fisher's Thermo Shandon brand Cryomatrix. We will order this for next time and I will try and re-do the embedding after thawing it. Should I lightly rinse the block and pat-dry before re-embedding? Thank you, Saro -----Original Message----- From: Joel Israel [mailto:JoelI@mcclainlab.com] Sent: March 22, 2011 9:22 AM To: Bascaramurty, Saro Subject: RE: [Histonet] Frozen sectioning of pig skin Did you thaw the tissue before you embedded in media? If not, try it. I use NEG-50 from Richard-Allan Scientific to embed frozen tissue. I have found it to be superior to others. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bascaramurty, Saro Sent: Tuesday, March 22, 2011 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen sectioning of pig skin I have sectioned some formalin fixed, paraffin embedded pig skin blocks in the past, but never the fresh frozen pig skin blocks. The samples were frozen by the researcher in liquid nitrogen, but not covered in cryomatrix and have been stored in the deep freezer for over a year. I have now embedded those blocks in cryomatrix and wanted to prepare sections at 30um and 6-8um thickness. When I tried it, I had problems getting good quality sections in spite of using brand new blades. I tried lowering the temperature from -18 deg.C to -22 / -25 deg.C. I still couldn't get quality sections consistently. If anyone from histoland has some good tips to improve the quality of these sections, I would greatly appreciate it. Thank you, Saro Bascaramurty Technical Officer Institute for Biodiagnostics National Research Council 435 Ellice Avenue, Winnipeg, Manitoba. R3B 1Y6 Tel: 204-984-7166 Fax:204-984-6978 email:saro.bascaramurty@nrc-cnrc.gc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hhawkins <@t> UTMB.EDU Tue Mar 22 10:02:32 2011 From: hhawkins <@t> UTMB.EDU (Hawkins, Hal K.) Date: Tue Mar 22 10:03:10 2011 Subject: [Histonet] comprehensive list? In-Reply-To: References: <44F1D6D7EB8CC84F92859EE5C4E6ECB401611077DB3D@CVMMBX.vetmed.wsu.edu> <535303.82446.qm@web65701.mail.ac4.yahoo.com>, Message-ID: <18DADD3ED840AC46AB24616A927CD0DB1E76C89E68@EXCHANGE2.utmb.edu> The list is still available online from Novocastra. Here is the link: http://www.ebiotrade.com/buyf/Novocastra/data/xreact/xreact.pdf ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg [pruegg@ihctech.net] Sent: Monday, March 21, 2011 11:48 AM To: 'Rene J Buesa'; 'Histonet'; 'TomTruscott' Subject: RE: [Histonet] comprehensive list? Novacastra had a cross reactivity list for their abs which I find very helpful, I have the old list but can't seem to find anyone who knows about it recently, I got it years ago when the instrument was still with the AU Co. Vision BioSystems, I will ask Leica reps to see if they know about this list. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, March 18, 2011 11:30 AM To: Histonet; TomTruscott Subject: Re: [Histonet] comprehensive list? Try your DAKO representative. NOVOCASTRA also used to have such a list for their Abs. Ren? J. --- On Fri, 3/18/11, Truscott, Tom wrote: From: Truscott, Tom Subject: [Histonet] comprehensive list? To: "Histonet" Date: Friday, March 18, 2011, 12:02 PM Hi All, Is there a comprehensive list in one location somewhere that gives information on all or most antibodies on how they work with FFPE or FS or other fixatives, so that we don't have to try to many approaches when we get a new antibody to try. Thanks, Tom T _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Mar 22 10:19:47 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Mar 22 10:20:59 2011 Subject: [Histonet] how to bleach melanoma cases without bad effect on IHC In-Reply-To: <000f01cbe899$a4fa79b0$eeef6d10$@com> References: <457061.45898.qm@web35604.mail.mud.yahoo.com>, <000f01cbe899$a4fa79b0$eeef6d10$@com> Message-ID: We have actually performed a bleach step before staining. The only affect I have seen ( and we have only done a few antibodies) is that the tissue gets pretty chewed up. Especially if there is a pretreatment step prior to the IHC staining. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse [cpyse@x-celllab.com] Sent: Tuesday, March 22, 2011 10:01 AM To: 'Shahram Sabeti'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] how to bleach melanoma cases without bad effect on IHC Sabeti I use the AEC chromogen (Dako catalog #K346989) for my melanoma cases. I use a aqueous mounting media, placed on the tissue only, dry it completely on a hot plate. When dry I coverslip it with permanent mounting media(don't place it in xylene), dry coverslip. Works great, and the color doesn't fade. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shahram Sabeti Sent: Tuesday, March 22, 2011 4:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how to bleach melanoma cases without bad effect on IHC hello dear fellows, i have an IHC project on paraffin embedded melanoma cases; some of them being heavily pigmented. i have seen many protocols based on H2O2 to bleach the pigmented ones, but i am not sure about any possible effect on antigen retrieval.please guide me . yours, sabeti _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Tue Mar 22 10:23:47 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Mar 22 10:24:02 2011 Subject: [Histonet] Frozen sectioning of pig skin In-Reply-To: References: Message-ID: <7457CD24-7DC2-486D-837A-E1ABFDB0C41E@email.arizona.edu> Saro, I'm certain there are differing thoughts on what to do about your tissue. Was the tissue fixed before it was frozen? How was it stored for the year it was in the freezer? Could be you have a piece of freeze dried pig skin that (if it cuts at all) will look awful. Here is what I would do and I'll probably take some heat (no pun intended) on this but this is what I would do: I'd drop the frozen chunk into fixative and let it thaw and then fix - time depends on the size of the chunk. After fixing I'd rinse the tissue, blot and drop into sucrose to cryoprotect. It is done when it sinks to the bottom of the sucrose. Blot. Then I would re-freeze in a frozen embedding medium like OCT or whatever you have - I like to put the tissue into some OCT and let it sit there for a while before freezing to absorb some of the OCT. I've had difficulty when there is sucrose solution on the outside of the tissue when it is frozen - makes it hard to get a decent section and there is much cursing going on in the lab. Andi Grantham On Mar 22, 2011, at 7:19 AM, Bascaramurty, Saro wrote: > No, I did not. We are using Fisher's Thermo Shandon brand Cryomatrix. We will order this for next time and I will try and re-do the embedding after thawing it. Should I lightly rinse the block and pat-dry before re-embedding? > > Thank you, > Saro > > -----Original Message----- > From: Joel Israel [mailto:JoelI@mcclainlab.com] > Sent: March 22, 2011 9:22 AM > To: Bascaramurty, Saro > Subject: RE: [Histonet] Frozen sectioning of pig skin > > Did you thaw the tissue before you embedded in media? If not, try it. > I use NEG-50 from Richard-Allan Scientific to embed frozen tissue. I have found it to be superior to others. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bascaramurty, Saro > Sent: Tuesday, March 22, 2011 10:00 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Frozen sectioning of pig skin > > > I have sectioned some formalin fixed, paraffin embedded pig skin blocks in the past, but never the fresh frozen pig skin blocks. The samples were frozen by the researcher in liquid nitrogen, but not covered in cryomatrix and have been stored in the deep freezer for over a year. I have now embedded those blocks in cryomatrix and wanted to prepare sections at 30um and 6-8um thickness. When I tried it, I had problems getting good quality sections in spite of using brand new blades. I tried lowering the temperature from -18 deg.C to -22 / -25 deg.C. I still couldn't get quality sections consistently. If anyone from histoland has some good tips to improve the quality of these sections, I would greatly appreciate it. > > > Thank you, > > Saro Bascaramurty > > Technical Officer > Institute for Biodiagnostics > National Research Council > 435 Ellice Avenue, > Winnipeg, Manitoba. R3B 1Y6 > > Tel: 204-984-7166 > Fax:204-984-6978 > email:saro.bascaramurty@nrc-cnrc.gc.ca > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MMargiotta <@t> bmhmc.org Tue Mar 22 10:30:12 2011 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Tue Mar 22 10:30:17 2011 Subject: [Histonet] muscle bxs Message-ID: Hi All, Quick question for those of you that do muscle biopsies. Do you make up the 4% paraformaldehyde or buy it from a vendor. If you make it up, would you share the recipe for it, please? If you purchase it, which vendor do you use? Thanks, Michele BMHMC DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From ASelf <@t> georgetownhospitalsystem.org Tue Mar 22 10:35:21 2011 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Tue Mar 22 10:35:27 2011 Subject: [Histonet] Ventilated Specimen Storage Message-ID: Hello Histonetters, How many of you have ventilated storage cabinets for storage of specimens? Thanks in advance for all your help, Amy GHS NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From tbeal <@t> cbgbiotech.com Tue Mar 22 10:39:45 2011 From: tbeal <@t> cbgbiotech.com (Teresa Beal) Date: Tue Mar 22 10:39:56 2011 Subject: [Histonet] Recycled Product Message-ID: <004201cbe8a7$600dca10$20295e30$@com> Hi. I am wondering if anyone is using recycled product w/ a Leica Bond immuno stainer. And if so, what kind of results are you getting? Any feedback would be appreciated! Thanks in advance Teresa Beal From wbenton <@t> cua.md Tue Mar 22 10:39:57 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Tue Mar 22 10:40:19 2011 Subject: [Histonet] RE: muscle bxs In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD91D6EC7CBB7@CUAEXH1.GCU-MD.local> Poly Scientific sells this Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele [MMargiotta@bmhmc.org] Sent: Tuesday, March 22, 2011 11:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] muscle bxs Hi All, Quick question for those of you that do muscle biopsies. Do you make up the 4% paraformaldehyde or buy it from a vendor. If you make it up, would you share the recipe for it, please? If you purchase it, which vendor do you use? Thanks, Michele BMHMC DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From algranth <@t> email.arizona.edu Tue Mar 22 10:55:10 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Mar 22 10:55:24 2011 Subject: [Histonet] Ventilated Specimen Storage In-Reply-To: References: Message-ID: It isn't Friday but I'm going on vacation at 2:50PM this afternoon so I'm taking the liberty. This reminds me of a funny CAP story...long time ago and in a galaxy far away (Iowa) we used to store or wet tissue after grossing in plastic garbage cans near a window. Over the years the cans looked a little worse for the wear and they were cracked and had some holes in them. When the CAP inspector asked my labmate is we stored the specimens in a ventilated containers she kicked a can and said, '"why of course we do". He was not amused. Andi On Mar 22, 2011, at 8:35 AM, Amy Self wrote: > Hello Histonetters, > > How many of you have ventilated storage cabinets for storage of specimens? > > Thanks in advance for all your help, > > Amy > GHS > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWeems <@t> sjha.org Tue Mar 22 11:26:49 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Mar 22 11:26:56 2011 Subject: [Histonet] Ventilated Specimen Storage In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081F17447D@CHEXCMS10.one.ads.che.org> Some people just have no sense of humor!! Glad it didn't fall apart with the kick. Reminded me of when the safey office came around asking what "RACE" stood for. He didn't think it was funny at all when I said, "Run around carelessly everywhere".... Happy Tuesday, ya'll! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rsrichmond <@t> gmail.com Tue Mar 22 11:43:50 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Mar 22 11:43:55 2011 Subject: [Histonet] Re: Grossing Station Recommendation Message-ID: I can't recommend any specific brand - it's rare to see a grossing station in the small pathology services I work in - but if I were buying one I'd want: 1. First and foremost, adequate ventilation - a louvered vent at hand level that pulls the formaldehyde fumes away from your hands as you work. No overhead kitchen hoods. 2. I think the words "ergonomic" and "pathologist' aren't supposed to be used in the same sentence, but it's awfully nice to have the table rack up and down for pathologists of varying heights. If that isn't possible, at least some sort of elevated table. 3. Most grossing stations are inadequately lighted, at least for my elderly eyes. 4. I don't like to gross with water running over the table and spraying potentially infectious aerosols up into my face. Bob Richmond Samurai Pathologist Knoxville TN From cjbulmer <@t> sbcglobal.net Tue Mar 22 11:51:33 2011 From: cjbulmer <@t> sbcglobal.net (Cindy Bulmer) Date: Tue Mar 22 11:51:36 2011 Subject: [Histonet] HercepTest Message-ID: <614879.51084.qm@web82307.mail.mud.yahoo.com> Hello Histoland, ? Anyone using the HercepTest for the Dako autostainer, Code # K5207? If so, what equipment are you using for the Epitope retrieval process? ? Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX From cjbulmer <@t> sbcglobal.net Tue Mar 22 12:12:12 2011 From: cjbulmer <@t> sbcglobal.net (Cindy Bulmer) Date: Tue Mar 22 12:12:15 2011 Subject: [Histonet] HercepTest Message-ID: <958001.91382.qm@web82302.mail.mud.yahoo.com> I understand the HercepTest calls for a waterbath for the epitope retrieval process... but, I'm wanting to know what type of equipment, ie:? waterbath make and model or if people are using the Dako PT link module. ? Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX From liz <@t> premierlab.com Tue Mar 22 12:15:06 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Mar 22 12:15:11 2011 Subject: [Histonet] HercepTest In-Reply-To: <958001.91382.qm@web82302.mail.mud.yahoo.com> Message-ID: We use the PT Link unit for Hercept Test Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy Bulmer Sent: Tuesday, March 22, 2011 11:12 AM To: Histonet Subject: [Histonet] HercepTest I understand the HercepTest calls for a waterbath for the epitope retrieval process... but, I'm wanting to know what type of equipment, ie:? waterbath make and model or if people are using the Dako PT link module. ? Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Tue Mar 22 12:24:37 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Mar 22 12:24:48 2011 Subject: [Histonet] HercepTest In-Reply-To: <958001.91382.qm@web82302.mail.mud.yahoo.com> References: <958001.91382.qm@web82302.mail.mud.yahoo.com> Message-ID: <003601cbe8b6$053b5ee0$0fb21ca0$@com> Cindy I use a Fisher Isotemp 205 waterbath. The end result is beautiful. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy Bulmer Sent: Tuesday, March 22, 2011 1:12 PM To: Histonet Subject: [Histonet] HercepTest I understand the HercepTest calls for a waterbath for the epitope retrieval process... but, I'm wanting to know what type of equipment, ie:? waterbath make and model or if people are using the Dako PT link module. ? Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srishan <@t> mail.holyname.org Tue Mar 22 12:33:12 2011 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Tue Mar 22 12:33:47 2011 Subject: [Histonet] vytec formalin neutralizer Message-ID: Hi Everyone, Is there any one out there who is neutralizing the 10% formalin with Vytec neutralizer? How are you disposing the formalin after neutralization? Need some information regarding this. Thanks in advance Nirmala Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From Ashley.Troutman <@t> Vanderbilt.Edu Tue Mar 22 12:47:08 2011 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Tue Mar 22 12:47:13 2011 Subject: [Histonet] HercepTest Message-ID: <7B310892042DA74CB3590053F424CFE613A57366F4@ITS-HCWNEM06.ds.Vanderbilt.edu> Hi Cindy, The protocol is very specific, requiring you use a water bath @ 95 deg for 40 minutes (I think) in the epitope retrieval solution provided. Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 (Office) 615-875-3311 (Lab) 615-343-9134 Message: 9 Date: Tue, 22 Mar 2011 09:51:33 -0700 (PDT) From: Cindy Bulmer Subject: [Histonet] HercepTest To: Histonet Message-ID: <614879.51084.qm@web82307.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello Histoland, ? Anyone using the HercepTest for the Dako autostainer, Code # K5207? If so, what equipment are you using for the Epitope retrieval process? ? Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX From jshelley <@t> sanfordburnham.org Tue Mar 22 12:58:34 2011 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Tue Mar 22 12:58:40 2011 Subject: [Histonet] Verhoeff/Masson's Stain Message-ID: Hello Histonetters, I have been asked to work on a project that will require me to do a Verhoeff/Masson's stain. I have run some samples through the stain from a protocol that I found on the archives and it is not working to my satisfaction. I am not getting the nice elastin fibers to show black like they should. I have looked at the slides just before going into 2% ferric chloride and it appears to be dull or limited staining. I am using a kit from PolyScientific and not sure if that might be my problem or not. My question is, are there another people who are running this special stain combo and would you be willing to share the procedure and also who you are buying your reagents from to accomplish the staining. I appreciate any help with this request. Thanks!!! Kind Regards! John J Shelley From DKBoyd <@t> chs.net Tue Mar 22 13:00:41 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Mar 22 13:02:31 2011 Subject: [Histonet] Ventilated Specimen Storage In-Reply-To: Message-ID: We do. One in Histology and one in the Morgue. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Amy Self Sent by: histonet-bounces@lists.utsouthwestern.edu 03/22/2011 11:37 AM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] Ventilated Specimen Storage Hello Histonetters, How many of you have ventilated storage cabinets for storage of specimens? Thanks in advance for all your help, Amy GHS NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From liz <@t> premierlab.com Tue Mar 22 13:04:31 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Mar 22 13:05:05 2011 Subject: [Histonet] Verhoeff/Masson's Stain In-Reply-To: Message-ID: John We do this stain all of the time, but we make up our reagents from scratch we don't use a kit. My suggestion is to make up your elastic stain fresh. We make up our elastic stain fresh and use it only once (or for that day) and then discard. I have attached our procedure in a different e-mail. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Tuesday, March 22, 2011 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Verhoeff/Masson's Stain Hello Histonetters, I have been asked to work on a project that will require me to do a Verhoeff/Masson's stain. I have run some samples through the stain from a protocol that I found on the archives and it is not working to my satisfaction. I am not getting the nice elastin fibers to show black like they should. I have looked at the slides just before going into 2% ferric chloride and it appears to be dull or limited staining. I am using a kit from PolyScientific and not sure if that might be my problem or not. My question is, are there another people who are running this special stain combo and would you be willing to share the procedure and also who you are buying your reagents from to accomplish the staining. I appreciate any help with this request. Thanks!!! Kind Regards! John J Shelley _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshelley <@t> sanfordburnham.org Tue Mar 22 13:14:20 2011 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Tue Mar 22 13:14:56 2011 Subject: [Histonet] RE: Verhoeff/Masson's Stain In-Reply-To: <9BF995BC0E47744E9673A41486E24EE238D4DA9908@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE238D4DA9908@MERCERMAIL.MercerU.local> Message-ID: Hi Shirley, I do make it fresh each time and the reagents are brand new from the company. I am using an appendix as my control and trying to get my hands on some aorta but I really think I should see some elastin fiber staining in the appendix. Kind Regards! ? John J Shelley -----Original Message----- From: Shirley A. Powell [mailto:POWELL_SA@mercer.edu] Sent: Tuesday, March 22, 2011 2:04 PM To: John Shelley Subject: RE: Verhoeff/Masson's Stain Your problem may be that the elastic stain should be made fresh every time, or you are differentiating in ferric chloride too long. Does the elastic stain get mixed right before use? Also check the expiration date of the kit. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Tuesday, March 22, 2011 1:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Verhoeff/Masson's Stain Hello Histonetters, I have been asked to work on a project that will require me to do a Verhoeff/Masson's stain. I have run some samples through the stain from a protocol that I found on the archives and it is not working to my satisfaction. I am not getting the nice elastin fibers to show black like they should. I have looked at the slides just before going into 2% ferric chloride and it appears to be dull or limited staining. I am using a kit from PolyScientific and not sure if that might be my problem or not. My question is, are there another people who are running this special stain combo and would you be willing to share the procedure and also who you are buying your reagents from to accomplish the staining. I appreciate any help with this request. Thanks!!! Kind Regards! John J Shelley _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michelecarr10 <@t> yahoo.com Tue Mar 22 13:29:35 2011 From: michelecarr10 <@t> yahoo.com (Michele Carr) Date: Tue Mar 22 13:29:38 2011 Subject: [Histonet] chemicals that are made in the lab Message-ID: <380454.27434.qm@web120712.mail.ne1.yahoo.com> Hi everyone, just was wondering how you determine the expiration dates for the graded alcohols in the lab that you make.? Do you take the expiration date off of the reagent alcohol bottles? Also, I use a recycler and recycle Xylene and Alcohol, what expiration dates do you all choose for these? Thanks in advance, Michele Carr From fbozkurt <@t> gmail.com Tue Mar 22 13:29:41 2011 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Tue Mar 22 13:29:44 2011 Subject: [Histonet] about decalcification Message-ID: Hello Histonet, Does anyone know procedure of formic acid with sodium citrate decalcification method. ? i need to all of steps of procudure; fixation, decalcification and tissue processing. I will decalcificate rabbit extremites. Thanks in advance. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 From esarricks <@t> gmail.com Tue Mar 22 13:45:10 2011 From: esarricks <@t> gmail.com (Erin Sarricks) Date: Tue Mar 22 13:45:32 2011 Subject: [Histonet] Mycoplasma stain Message-ID: Hi Histonet- Does anyone know of a good stain for mycoplasma? Our pathologist wanted to try an overnight Giemsa stain, but I wanted to check and see if anyone knew of another stain that might work well. Thanks! Erin Sarricks, HT (ASCP) Histology Laboratory Technician USAMRICD Comparative Pathology Branch Office: Bldg E-3081 Room 178 E-mail: erin.p.sarricks@us.army.mil Phone: 410-436-1967 From llewllew <@t> shaw.ca Tue Mar 22 13:48:07 2011 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Mar 22 13:48:14 2011 Subject: [Histonet] Verhoeff/Masson's Stain In-Reply-To: References: Message-ID: <4D88EEE7.6010807@shaw.ca> Verhoeff's staining solution uses an iron mordasnt, which is an oxidising agent, and iodine, another oxidising agent. It is very easy to overoxidise the hematoxylin, particularly if a stock alcoholic solution is used. I always used to preweigh 1 gram hematoxylin and keep in test tubes, then add the required amount of ethanol at the time I made the working solution. Problems I had had with poor staining then stopped. Have you considered switching to an iron resorcin dye type method? They are progressive, much easier to control and work very consistently. Their drawback is that making the solution is a nuisance and staining often needs overnight. I liked Humberstone's variant (greeny blue black elastic) as the solution is stable for several years and improves over that time with shorter staining times. Others like Miller's (black elatic). There are others as well. Bryan Llewellyn John Shelley wrote: > Hello Histonetters, > > I have been asked to work on a project that will require me to do a Verhoeff/Masson's stain. I have run some samples through the stain from a protocol that I found on the archives and it is not working to my satisfaction. I am not getting the nice elastin fibers to show black like they should. I have looked at the slides just before going into 2% ferric chloride and it appears to be dull or limited staining. I am using a kit from PolyScientific and not sure if that might be my problem or not. My question is, are there another people who are running this special stain combo and would you be willing to share the procedure and also who you are buying your reagents from to accomplish the staining. I appreciate any help with this request. Thanks!!! > > Kind Regards! > > John J Shelley > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond <@t> gmail.com Tue Mar 22 13:55:32 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Mar 22 13:55:37 2011 Subject: [Histonet] Re: Ventilated Specimen Storage Message-ID: Amy Self asks: >>How many of you have ventilated storage cabinets for storage of specimens?<< I'm supposing that by "specimens" you mean surgical specimens stored in formalin in closed containers. I've worked in a lot of pathology services in my life, and I think I've never seen such a thing. Most services keep wet tissue for from two to four weeks. Is some regulatory agency going to require it? Bob Richmond Samurai Pathologist Knoxville TN From laurie.colbert <@t> huntingtonhospital.com Tue Mar 22 14:27:59 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Mar 22 14:28:03 2011 Subject: FW: [Histonet] Ventilated Specimen Storage Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AA2F@EXCHANGE3.huntingtonhospital.com> -----Original Message----- From: Laurie Colbert Sent: Tuesday, March 22, 2011 12:27 PM To: 'Amy Self' Subject: RE: [Histonet] Ventilated Specimen Storage We have a ventilated closet for storing our old specimens. Our lab is two years old, and this was something that I asked for specifically when they were designing the lab. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, March 22, 2011 8:35 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Ventilated Specimen Storage Hello Histonetters, How many of you have ventilated storage cabinets for storage of specimens? Thanks in advance for all your help, Amy GHS NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Mar 22 14:47:42 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Mar 22 14:48:00 2011 Subject: [Histonet] HercepTest In-Reply-To: <958001.91382.qm@web82302.mail.mud.yahoo.com> References: <958001.91382.qm@web82302.mail.mud.yahoo.com> Message-ID: We use the PT link, which according to dako is considered a waterbath. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy Bulmer [cjbulmer@sbcglobal.net] Sent: Tuesday, March 22, 2011 1:12 PM To: Histonet Subject: [Histonet] HercepTest I understand the HercepTest calls for a waterbath for the epitope retrieval process... but, I'm wanting to know what type of equipment, ie: waterbath make and model or if people are using the Dako PT link module. Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Tue Mar 22 16:04:16 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Tue Mar 22 16:04:21 2011 Subject: [Histonet] Stupid Rabbit primaries! Message-ID: So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From LSebree <@t> uwhealth.org Tue Mar 22 16:13:04 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Mar 22 16:13:08 2011 Subject: [Histonet] Stupid Rabbit primaries! In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E2738010029DE@UWHC-MAIL01.uwhis.hosp.wisc.edu> Do you know for sure that its the Ab and not endogenous biotin, peroxidase or Phosphatase? You can block for all those. Otherwise there are a number of commercial blocking agents on the market...try Biocare for a start (800)799-9499 or better yet, the company that makes your antibody. Good luck. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Tuesday, March 22, 2011 4:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid Rabbit primaries! So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Tue Mar 22 16:13:53 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Tue Mar 22 16:13:57 2011 Subject: [Histonet] Stupid Rabbit primaries! In-Reply-To: <8C023B4AB999614BA4791BAEB26E2738010029DE@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: <8C023B4AB999614BA4791BAEB26E2738010029DE@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: I blocked for all of those... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: Sebree Linda A [mailto:LSebree@uwhealth.org] Sent: Tuesday, March 22, 2011 4:13 PM To: Sarah Goebel; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Stupid Rabbit primaries! Do you know for sure that its the Ab and not endogenous biotin, peroxidase or Phosphatase? You can block for all those. Otherwise there are a number of commercial blocking agents on the market...try Biocare for a start (800)799-9499 or better yet, the company that makes your antibody. Good luck. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Tuesday, March 22, 2011 4:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid Rabbit primaries! So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Mar 22 18:20:43 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Mar 22 18:20:54 2011 Subject: [Histonet] RE: Verhoeff/Masson's Stain In-Reply-To: References: <9BF995BC0E47744E9673A41486E24EE238D4DA9908@MERCERMAIL.MercerU.local> Message-ID: <6D6BD1DE8A5571489398B392A38A71571883540D@xmdb02.nch.kids> Hi all, My experience with Verhoeff stain indicates that poor, muddy staining of elastic fibres results from using too fresh a solution of alcoholic haematoxylin. The usual methods require a 10% alcoholic Hx that is mixed with the other ingredients. I always let the stock 10% alcoholic Hx stand for at least a week before using. Too fresh Hx is also difficult to differentiate (sometimes less than 1 dip is required in 2% ferric chloride). As for controls, a section of skin is best - the odd artery will stain, but more importantly, the fine elastic fibres in the dermis should be easily seen. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Wednesday, 23 March 2011 5:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Verhoeff/Masson's Stain Hi Shirley, I do make it fresh each time and the reagents are brand new from the company. I am using an appendix as my control and trying to get my hands on some aorta but I really think I should see some elastin fiber staining in the appendix. Kind Regards! ? John J Shelley -----Original Message----- From: Shirley A. Powell [mailto:POWELL_SA@mercer.edu] Sent: Tuesday, March 22, 2011 2:04 PM To: John Shelley Subject: RE: Verhoeff/Masson's Stain Your problem may be that the elastic stain should be made fresh every time, or you are differentiating in ferric chloride too long. Does the elastic stain get mixed right before use? Also check the expiration date of the kit. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Tuesday, March 22, 2011 1:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Verhoeff/Masson's Stain Hello Histonetters, I have been asked to work on a project that will require me to do a Verhoeff/Masson's stain. I have run some samples through the stain from a protocol that I found on the archives and it is not working to my satisfaction. I am not getting the nice elastin fibers to show black like they should. I have looked at the slides just before going into 2% ferric chloride and it appears to be dull or limited staining. I am using a kit from PolyScientific and not sure if that might be my problem or not. My question is, are there another people who are running this special stain combo and would you be willing to share the procedure and also who you are buying your reagents from to accomplish the staining. I appreciate any help with this request. Thanks!!! Kind Regards! John J Shelley _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From AnthonyH <@t> chw.edu.au Tue Mar 22 18:23:04 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Mar 22 18:23:16 2011 Subject: [Histonet] Verhoeff/Masson's Stain In-Reply-To: <4D88EEE7.6010807@shaw.ca> References: <4D88EEE7.6010807@shaw.ca> Message-ID: <6D6BD1DE8A5571489398B392A38A715718835428@xmdb02.nch.kids> See there are always differing experiences (see my earlier post) How are you Bryan, well I hope? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Llewellyn Sent: Wednesday, 23 March 2011 5:48 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Verhoeff/Masson's Stain Verhoeff's staining solution uses an iron mordasnt, which is an oxidising agent, and iodine, another oxidising agent. It is very easy to overoxidise the hematoxylin, particularly if a stock alcoholic solution is used. I always used to preweigh 1 gram hematoxylin and keep in test tubes, then add the required amount of ethanol at the time I made the working solution. Problems I had had with poor staining then stopped. Have you considered switching to an iron resorcin dye type method? They are progressive, much easier to control and work very consistently. Their drawback is that making the solution is a nuisance and staining often needs overnight. I liked Humberstone's variant (greeny blue black elastic) as the solution is stable for several years and improves over that time with shorter staining times. Others like Miller's (black elatic). There are others as well. Bryan Llewellyn John Shelley wrote: > Hello Histonetters, > > I have been asked to work on a project that will require me to do a Verhoeff/Masson's stain. I have run some samples through the stain from a protocol that I found on the archives and it is not working to my satisfaction. I am not getting the nice elastin fibers to show black like they should. I have looked at the slides just before going into 2% ferric chloride and it appears to be dull or limited staining. I am using a kit from PolyScientific and not sure if that might be my problem or not. My question is, are there another people who are running this special stain combo and would you be willing to share the procedure and also who you are buying your reagents from to accomplish the staining. I appreciate any help with this request. Thanks!!! > > Kind Regards! > > John J Shelley > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From dmbcmp <@t> aol.com Tue Mar 22 20:34:30 2011 From: dmbcmp <@t> aol.com (dmbcmp@aol.com) Date: Tue Mar 22 20:34:38 2011 Subject: [Histonet] please remove me from list. Thank you. Message-ID: <8CDB711970BBFB6-960-31B96@webmail-d137.sysops.aol.com> Please remove me from list. Thank you. Dannie Blake From Pitts.Jaclyn <@t> mayo.edu Tue Mar 22 20:45:39 2011 From: Pitts.Jaclyn <@t> mayo.edu (Pitts, Jaclyn S. (Jackie), HT(ASCP)) Date: Tue Mar 22 20:45:43 2011 Subject: [Histonet] B5 Message-ID: Hey all, I was just curious how many of you out there still use B5 as a fixative for bone marrows. Thank Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic Rochester, MN E-mail: pitts.jaclyn@mayo.edu From Timothy.Morken <@t> ucsfmedctr.org Tue Mar 22 21:00:25 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Mar 22 21:03:46 2011 Subject: [Histonet] RE: Stupid Rabbit primaries! In-Reply-To: References: Message-ID: Sarah, is it a whole serum antibody? Jackson Laboratories has Ig fragments that can be used to block endogenous Ig sites in the tissue and largely eliminate non-specific staining. A well-purified antibody prep should not give much background, and if it is affinity purified it should be almost as good as a monoclonal. Tim Morken ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com [sgoebel@mirnarx.com] Sent: Tuesday, March 22, 2011 2:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid Rabbit primaries! So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Wed Mar 23 03:31:38 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Wed Mar 23 03:32:17 2011 Subject: [Histonet] Stupid Rabbit primaries! In-Reply-To: References: Message-ID: Having read all the other comments, here's my 2.5 pence worth! Have you tried a background reducing antibody diluent? You can get these from DAKO or Menarini Diagnostics: http://www.dako.com/uk/ar38/p107410/prod_products.htm. Or MP-905-25 / MP-905-100, depending on the volume you require, from Menarini Diagnostics, website http://www.dako.com/uk/ar38/p107410/prod_products.htm. I found these useful for rabbit polyclonals. Good luck Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: 22 March 2011 21:04 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid Rabbit primaries! So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sohail_e <@t> yahoo.com Wed Mar 23 05:47:34 2011 From: sohail_e <@t> yahoo.com (Sohail Ejaz) Date: Wed Mar 23 05:47:38 2011 Subject: [Histonet] Rabbit Anti-NeuN Message-ID: <507952.52433.qm@web39508.mail.mud.yahoo.com> Dear All? I am looking for?Rabbit Anti- NeuN antiboy to use it with anti-mouse OX42 for double immuno staining. Is there any one who have used any?Rabbit Anti- NeuN? Please let me know. Thanks Sohail From trathborne <@t> somerset-healthcare.com Wed Mar 23 07:42:55 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Mar 23 07:45:20 2011 Subject: [Histonet] B5 In-Reply-To: Message-ID: We do not. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Pitts, Jaclyn S. (Jackie), HT(ASCP) Sent: Tuesday, March 22, 2011 9:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 Hey all, I was just curious how many of you out there still use B5 as a fixative for bone marrows. Thank Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic Rochester, MN E-mail: pitts.jaclyn@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Jeffery.Miller <@t> spectrum-health.org Wed Mar 23 07:56:20 2011 From: Jeffery.Miller <@t> spectrum-health.org (Jeffery.Miller@spectrum-health.org) Date: Wed Mar 23 07:56:31 2011 Subject: [Histonet] RE: B5 In-Reply-To: References: Message-ID: We did until about 7 yrs ago when the hospital decided that all mercury (thermometers and chemicals) would no longer be allowed which was fine with us. We just went to 10% NBF since then. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pitts, Jaclyn S. (Jackie), HT(ASCP) Sent: Tuesday, March 22, 2011 9:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 Hey all, I was just curious how many of you out there still use B5 as a fixative for bone marrows. Thank Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic Rochester, MN E-mail: pitts.jaclyn@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aobrien88 <@t> comcast.net Wed Mar 23 08:02:59 2011 From: aobrien88 <@t> comcast.net (aobrien88@comcast.net) Date: Wed Mar 23 08:03:02 2011 Subject: [Histonet] Pax 2 Message-ID: <1378322132.2177590.1300885379314.JavaMail.root@sz0008a.emeryville.ca.mail.comcast.net> Is anyone using the Pax 2 antibody?? Who is your vendor and could you share some information about your procedure? Thank you, Andrea O'Brien UroPartners From cgill <@t> marylandgeneral.org Wed Mar 23 08:07:25 2011 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Wed Mar 23 08:07:33 2011 Subject: [Histonet] Ventilated Specimen Storage In-Reply-To: References: Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9F52@MDGEN-EXCH1.marylandgeneral.org> We have one in our gross room. Caula Gill(HT)ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, March 22, 2011 11:35 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Ventilated Specimen Storage Hello Histonetters, How many of you have ventilated storage cabinets for storage of specimens? Thanks in advance for all your help, Amy GHS NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Mar 23 08:23:26 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Mar 23 08:23:29 2011 Subject: [Histonet] please remove me from list. Thank you. In-Reply-To: <8CDB711970BBFB6-960-31B96@webmail-d137.sysops.aol.com> References: <8CDB711970BBFB6-960-31B96@webmail-d137.sysops.aol.com> Message-ID: No. And you're not welcome. It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Tue, Mar 22, 2011 at 9:34 PM, wrote: > > > Please remove me from list. Thank you. > Dannie Blake > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MLunetta <@t> luhcares.org Wed Mar 23 08:25:34 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Wed Mar 23 08:25:55 2011 Subject: [Histonet] Re: vytec formalin neutralizer Message-ID: <4D89A06E020000A800058E32@ns.luhcares.org> Nirmala We Use Vytec and after consulting with the city's public utility it is ok for us to dispose of the waste with copious amounts of water down the drain. So, I would contact you local water authority and see if you can do the same. Regards, Matt Lunetta BS HT(ASCP)cm Longmont United Hospital Longmont, Colorado Message: 4 Date: Tue, 22 Mar 2011 10:33:12 -0700 From: srishan@mail.holyname.org Subject: [Histonet] vytec formalin neutralizer To: histonet-bounces@lists.utsouthwestern.edu, Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi Everyone, Is there any one out there who is neutralizing the 10% formalin with Vytec neutralizer? How are you disposing the formalin after neutralization? Need some information regarding this. Thanks in advance Nirmala From dgoodwin <@t> rwjuhh.edu Wed Mar 23 08:37:32 2011 From: dgoodwin <@t> rwjuhh.edu (Goodwin, Diana) Date: Wed Mar 23 08:37:36 2011 Subject: [Histonet] Underfixation of breast tissue may lead to false results? Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71E70A6D949@HAMEXMBA.rwjham.local> Dear Histonetters: I recently read the following in an article on Medscape: "Underfixation of breast tissue may lead to false negative ER results and false-positive HER2 results. In these situations, the tissue is actually fixed in 100% ethanol, which is used to dehydrate the specimens after fixation." Can anyone explain? Diana Goodwin Supervisor, Histology Laboratory xt. 6996 From laurie.colbert <@t> huntingtonhospital.com Wed Mar 23 09:22:39 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Mar 23 09:22:42 2011 Subject: [Histonet] B5 In-Reply-To: References: Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AA31@EXCHANGE3.huntingtonhospital.com> We do! Our pathologists aren't interested in using any other fixative. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pitts, Jaclyn S. (Jackie), HT(ASCP) Sent: Tuesday, March 22, 2011 6:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 Hey all, I was just curious how many of you out there still use B5 as a fixative for bone marrows. Thank Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic Rochester, MN E-mail: pitts.jaclyn@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Mar 23 09:35:15 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Mar 23 09:35:20 2011 Subject: [Histonet] B5 In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2AA31@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D76830AD2AA31@EXCHANGE3.huntingtonhospital.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081F17460A@CHEXCMS10.one.ads.che.org> Who do you get to discard it? Do you pay a fortune? j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, March 23, 2011 10:23 To: Pitts, Jaclyn S. (Jackie), HT(ASCP); histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] B5 We do! Our pathologists aren't interested in using any other fixative. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pitts, Jaclyn S. (Jackie), HT(ASCP) Sent: Tuesday, March 22, 2011 6:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 Hey all, I was just curious how many of you out there still use B5 as a fixative for bone marrows. Thank Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic Rochester, MN E-mail: pitts.jaclyn@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From relia1 <@t> earthlink.net Wed Mar 23 10:32:36 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Mar 23 10:32:36 2011 Subject: [Histonet] RELIA Histology Careers Bulletin 3/23/2011 March Madness is Here!! Message-ID: <0F90F568F3944D3C870F289E1675FF48@ownerf1abaad51> Hi Histonetters! I hope you had a fun and lucky St. Patricks Day. March Madness has started and in addition to some great College Basketball. I have been experiencing the madness as well. My phone has literally been ringing off the hook with some great new opportunities. All of these positions are permanent full time positions and my clients offer excellent compensation, benefits and relocation assistance. Best of all these clients are motivated to hire and eager to meet you! Here is a list of my current openings: HISTOLOGY/PATHOLOGY MANAGEMENT ME ? Portland Histology Lab Supervisor (Night shift) NC ? Asheville Histology Lab Manager FL ? Sarasota Histology Supervisor/Lead Tech CA ? Modesto ? Histology Lab Manager NH ? Manchester Cytology/Histology Supervisor LA ? Baton Rouge Early Morning Histology Supervisor HISTOTECHS NC ? Charlotte Mohs Histotech TX ? Dallas Night Shift Histotech TX ? Tyler Dermpath Histotech ? New grads welcome! LA ? Lafayette Night Shift Lead Tech OR ? Portland Night Shift Histotech MA ? Cape Cod Histotech MA ? Peabody Night Shift Histotech FL ? Fort Myers Mohs Histotech FL ? Sarasota Dermpath histotech If you or anyone you know might be interested in any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net. Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From llewllew <@t> shaw.ca Wed Mar 23 12:28:08 2011 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Wed Mar 23 12:28:18 2011 Subject: [Histonet] Underfixation of breast tissue may lead to false results? In-Reply-To: <09411E0112A96A459D8D5FBDAB9C15C71E70A6D949@HAMEXMBA.rwjham.local> References: <09411E0112A96A459D8D5FBDAB9C15C71E70A6D949@HAMEXMBA.rwjham.local> Message-ID: <4D8A2DA8.1070700@shaw.ca> This has been commented on several times by old fogey histotechs in the past. Any formalin variant takes some time to actually chemically alter (fix) the tissue. Usually this is a minimum of 24 hours for a 3-4 mm thick piece. In modern labs there is a focus on 24 hour or less turnaround time, so formalin fixation is usually minimalist and may be inadequate, as in this case. After all, the universe will not change itself for our benefit just because we want fast results. This means that underfixed tissues with just a few hours in formalin are processed. The unfixed components are exposed to ethanol during this process. Remember that ethanol is also a (very poor) fixing agent, so the inadequatly fixed tissue is fixed by it, with all the characteristics ethanol fixation gives, shattering, hardening, distortion, etc. This is frequently referred to as "overprocessing", but it is just poor fixation from ethanol. For most tissues, wetting the block surface allows reasonable sections to be cut, but that is only a quick fix. Procedures which require tissues to be properly fixed may not be successful as a consequence. Bryan Llewellyn Goodwin, Diana wrote: > Dear Histonetters: > > I recently read the following in an article on Medscape: > > "Underfixation of breast tissue may lead to false negative ER results and false-positive HER2 results. In these situations, the tissue is actually fixed in 100% ethanol, which is used to dehydrate the specimens after fixation." > > Can anyone explain? > > Diana Goodwin > Supervisor, Histology Laboratory > xt. 6996 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TroyerDA <@t> EVMS.EDU Wed Mar 23 13:12:21 2011 From: TroyerDA <@t> EVMS.EDU (Troyer, Dean A.) Date: Wed Mar 23 13:12:25 2011 Subject: [Histonet] Underfixation of breast tissue may lead to falseresults? References: <09411E0112A96A459D8D5FBDAB9C15C71E70A6D949@HAMEXMBA.rwjham.local> <4D8A2DA8.1070700@shaw.ca> Message-ID: <71EE6DB05CB563458E8CBA495B3E4BD003F562C5@romulus.evms.net> Just wanted to qualify somewhat the observation that alcohol is a poor fixative. Alcohol can be very effective as a primary fixative. I agree formalin isn't likely to be displaced in this country soon, but aqueous ethanol or methanol is a viable alternative that has merits worth considering. Dean Troyer Eastern Virginia Medical School ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bryan Llewellyn Sent: Wed 3/23/2011 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Underfixation of breast tissue may lead to falseresults? This has been commented on several times by old fogey histotechs in the past. Any formalin variant takes some time to actually chemically alter (fix) the tissue. Usually this is a minimum of 24 hours for a 3-4 mm thick piece. In modern labs there is a focus on 24 hour or less turnaround time, so formalin fixation is usually minimalist and may be inadequate, as in this case. After all, the universe will not change itself for our benefit just because we want fast results. This means that underfixed tissues with just a few hours in formalin are processed. The unfixed components are exposed to ethanol during this process. Remember that ethanol is also a (very poor) fixing agent, so the inadequatly fixed tissue is fixed by it, with all the characteristics ethanol fixation gives, shattering, hardening, distortion, etc. This is frequently referred to as "overprocessing", but it is just poor fixation from ethanol. For most tissues, wetting the block surface allows reasonable sections to be cut, but that is only a quick fix. Procedures which require tissues to be properly fixed may not be successful as a consequence. Bryan Llewellyn Goodwin, Diana wrote: > Dear Histonetters: > > I recently read the following in an article on Medscape: > > "Underfixation of breast tissue may lead to false negative ER results and false-positive HER2 results. In these situations, the tissue is actually fixed in 100% ethanol, which is used to dehydrate the specimens after fixation." > > Can anyone explain? > > Diana Goodwin > Supervisor, Histology Laboratory > xt. 6996 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Wed Mar 23 15:00:33 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Mar 23 15:00:39 2011 Subject: [Histonet] RE: Stupid Rabbit Primary Antibodies In-Reply-To: References: Message-ID: You might try Background Sniper from Biocare or Background Buster from Innovex - we process animal tissues exclusively and we use several rabbit primary antibodies. Tresa Goins, Ph.D. Histopathology Supervisor Department of Livestock Bozeman, Montana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Tuesday, March 22, 2011 3:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid Rabbit primaries! So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pitts.Jaclyn <@t> mayo.edu Wed Mar 23 16:44:17 2011 From: Pitts.Jaclyn <@t> mayo.edu (Pitts, Jaclyn S. (Jackie), HT(ASCP)) Date: Wed Mar 23 16:44:22 2011 Subject: [Histonet] RE: B5 In-Reply-To: References: Message-ID: Thanks. I am not sure who disposes of the B5 for us but I do know that it would be much easier if we changed to something else. Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic E-mail: pitts.jaclyn@mayo.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffery.Miller@spectrum-health.org Sent: Wednesday, March 23, 2011 7:56 AM To: Pitts, Jaclyn S. (Jackie), HT(ASCP); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: B5 We did until about 7 yrs ago when the hospital decided that all mercury (thermometers and chemicals) would no longer be allowed which was fine with us. We just went to 10% NBF since then. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pitts, Jaclyn S. (Jackie), HT(ASCP) Sent: Tuesday, March 22, 2011 9:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 Hey all, I was just curious how many of you out there still use B5 as a fixative for bone marrows. Thank Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic Rochester, MN E-mail: pitts.jaclyn@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Mar 23 17:22:05 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Mar 23 17:22:09 2011 Subject: [Histonet] Re: Verhoeff/Masson's Stain Message-ID: The comments on Verhoeff's hematoxylin should be sufficient to trouble-shoot this cumbersome but beautiful old stain - I don't know a stain I'd rather photograph, except maybe Ram?n y Cajal's gold sublimate. Verhoeff's hematoxylin is a century old this year. The original paper is in the JAMA, of all places - I think I had a photocopy once. (We need a commemorative postage stamp!) Frederick Herman Verhoeff (a.k.a. Freddy, 1874-1968 - I've always heard it pronounced veer-hoff) was the founder of American ophthalmologic pathology. Working solo in a little lab at the Massachusetts Eye and Ear Infirmary, he published a great number of papers in ophthalmology. He intended his stain to demonstrate myelin sheaths in human autopsy optic nerve. It was in routine use for this purpose at the Wilmer Eye Institute at Johns Hopkins when I was a pathology resident there in the later 1960's, when Dick Green (died last year at 76) was the chief of eye pathology. Bob Richmond Samurai Pathologist Knoxville TN From lpwenk <@t> sbcglobal.net Wed Mar 23 21:26:29 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Mar 23 21:26:34 2011 Subject: [Histonet] B5 In-Reply-To: References: Message-ID: We switched a few years ago. Hospital went mercury free, and we couldn't find any company in 6 states that would take waste mercury. One common mistake when converting to a zinc formalin is to try to fix it the same time as the B5. Mercury binds/fixes very quickly, zinc is slower. It's chemistry, and there's not much you can do to make it go a whole lot faster. So, whatever time you fixed normally in B5, multiply it by 1.5 to 2.0 (2 hour B5 fix will now require 3-4 hours fixation in a Zinc formalin). If you don't fix long enough in the zinc formalin, the tissue is going to have smudgy pale blue nuclei on the bone marrow. Been there, done that when one of the med techs insisted on fixing in the zinc formalin for the same 2 hours as B5 because she didn't like her routine disrupted (B5 was 2 hours, so she insisted the zinc formalin should also be 2 hours). Once we showed her the difference in quality between 2 hours and 3 hours, and asked her which one she would like her daughter's bone marrow biopsy to look like, if we ever had to diagnose her for leukemia, she changed to 3 hours, and now the bone marrows look fine. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 478073 -------------------------------------------------- From: "Pitts, Jaclyn S. (Jackie), HT(ASCP)" Sent: Tuesday, March 22, 2011 9:45 PM To: Subject: [Histonet] B5 > Hey all, > I was just curious how many of you out there still use B5 as a fixative > for bone marrows. > Thank > > Jaclyn Pitts, HT(ASCP) > Histotechnician > Department of Laboratory Medicine and Pathology > Mayo Clinic Rochester, MN > E-mail: pitts.jaclyn@mayo.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From njblademaster <@t> gmail.com Wed Mar 23 22:10:06 2011 From: njblademaster <@t> gmail.com (Nathan Jentsch) Date: Wed Mar 23 22:10:29 2011 Subject: [Histonet] Re: chemicals that are made in the lab Message-ID: Michele, I would use the expiration dates from the original alcohol bottles for graded alcohols. Alcohol won't expire any faster just because you dilute it. I use two years from the month of recycling for an expiration date for recycled product. In reality these chemicals won't expire for decades as long as they're not contaminated, but a state inspector will want to see reasonable numbers, and I know we will use the chemicals long before the two year mark hits. Nathan Jentsch BS HT(ASCP) From LRaff <@t> uropartners.com Thu Mar 24 06:50:03 2011 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Thu Mar 24 06:50:08 2011 Subject: [Histonet] FW: Meeting Confirmation--correection Message-ID: This is 5 pm Chicago Time. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: Lester Raff MD Sent: Thursday, March 24, 2011 6:49 AM To: George Engel MD; Heather Brown; 'pamela papas' Subject: FW: Meeting Confirmation Note telephone conference for VetPathDX. This will be at 4 pm Chicago time on Tuesday. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -----Original Message----- From: Alfred Lui [mailto:info2@meetingwizard.com] Sent: Wednesday, March 23, 2011 10:16 PM To: Lester Raff MD Subject: Meeting Confirmation Hi, This is a confirmation from Alfred Lui for the following meeting event: Start Date/Time: Tuesday March 29, 2011 3:00 PM Pacific Time Subject/Purpose: VetPathDx Status Update Type/Format: face-to-face Duration: approximately 1 hour Location: Conference call phone number to be sent later You can view complete details by clicking the following link: http://www.meetingwizard.com/mwiz/v/rd.cfm?m=848475&mtag=341311609&g=840 7447 If you have not yet responded, or if you want to change your response, click the following link: http://www.meetingwizard.com/mwiz/v/r.cfm?m=848475&mtag=341311609&g=8407 447 ______________________________________________________ MeetingWizard - great meetings begin here! From JSCHUMA1 <@t> Fairview.org Thu Mar 24 07:18:23 2011 From: JSCHUMA1 <@t> Fairview.org (Schumacher, Jennifer J) Date: Thu Mar 24 07:18:30 2011 Subject: [Histonet] Ventilated Specimen Storage In-Reply-To: <57BE698966D5C54EAE8612E8941D76830AD2AA2F@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D76830AD2AA2F@EXCHANGE3.huntingtonhospital.com> Message-ID: <45EBFA1E7C931E45BBA7172D42F23C920987FC318E@EXCH-MBX5.Fairview.org> We have two of these units and love them. We also recently remodeled and made sure to include space for them in the gross room to reduce transport as well. Jen Schumacher -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, March 22, 2011 2:28 PM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Ventilated Specimen Storage -----Original Message----- From: Laurie Colbert Sent: Tuesday, March 22, 2011 12:27 PM To: 'Amy Self' Subject: RE: [Histonet] Ventilated Specimen Storage We have a ventilated closet for storing our old specimens. Our lab is two years old, and this was something that I asked for specifically when they were designing the lab. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, March 22, 2011 8:35 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Ventilated Specimen Storage Hello Histonetters, How many of you have ventilated storage cabinets for storage of specimens? Thanks in advance for all your help, Amy GHS NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From schlea <@t> sage.edu Thu Mar 24 07:27:54 2011 From: schlea <@t> sage.edu (Anna Schleifstein) Date: Thu Mar 24 07:27:58 2011 Subject: [Histonet] Histology equipment Message-ID: <21282647.60061300969674741.JavaMail.root@ccprodapp31> Sage Colleges has several pieces of older histology equipment no longer in use. They are as follows: AO Microtome Knife Sharpener model 925. (Includes cover and 1 glass hone plate), Tissue Tek Microtome/Cryostat model 4553 (with knife)) and AO Microtome model 820 (includes Specimen Holder, knife and Standard Knife Holder). All items are in great working condition. Please contact me if you are interested. All offers will be considered. Anna Schleifstein SCA Bio Lab Coordinator Sage Colleges Albany NY 12208 518- 292-1748 schlea@sage.edu From aevans3 <@t> lghealth.org Thu Mar 24 07:31:13 2011 From: aevans3 <@t> lghealth.org (Evans, Andria B) Date: Thu Mar 24 07:33:02 2011 Subject: [Histonet] Microsatellite Instability Antibodies Message-ID: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA4F@MAIL-AG-CLUSTER.lha.org> I am currently working up the MSI Antibodies (MLH1, MSH6, MSH2, and PMS2) and need some input on the protocol that you may be running in your lab and what clone(manufacture) you are using. We are currently running the Ventana IHC stainers with the UltraView Detection. Thanks for your help! Andria B Evans HTL(ASCP)CM This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From dcojita <@t> tampabay.rr.com Thu Mar 24 10:04:21 2011 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Thu Mar 24 10:04:25 2011 Subject: [Histonet] non-functioning equipment In-Reply-To: <21282647.60061300969674741.JavaMail.root@ccprodapp31> Message-ID: <20110324150421.UK18C.55475.root@hrndva-web12-z01> Does anyone have procedures for disposing of their old (non-working) equipment (i.e. paraffin pot). Is it ok to place items like that in a dumpster? From W.E.J.Hoekert <@t> olvg.nl Thu Mar 24 10:18:08 2011 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Thu Mar 24 10:18:16 2011 Subject: [Histonet] Microsatellite Instability Antibodies References: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA4F@MAIL-AG-CLUSTER.lha.org> Message-ID: <1190CB05C44B13409483514729C2FC360C0B7B@PAIT42.olvg.nl> >We are currently running the Ventana IHC stainers with the UltraView Detection. Just like us! MLH-1: Cell Marque (ref 760-4264). 30' cc1, 32' ab incubation. MSH-2: Cell Marque (ref 760-4265). 60' cc1, 60' ab inc. PMS2: Becton Dickinson, clone A16-4, cat nr 556415. 30' cc1, 32' ab inc. +amplification MSH6: Becton Dickinson, clone 44, cat nr 610919. 60' cc2, 60' ab inc. MSH-6 does not give very nice results, however, it works. Good Luck, Willem ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Evans, Andria B Verzonden: do 24-3-2011 13:31 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Microsatellite Instability Antibodies I am currently working up the MSI Antibodies (MLH1, MSH6, MSH2, and PMS2) and need some input on the protocol that you may be running in your lab and what clone(manufacture) you are using. We are currently running the Ventana IHC stainers with the UltraView Detection. Thanks for your help! Andria B Evans HTL(ASCP)CM This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From JWeems <@t> sjha.org Thu Mar 24 10:48:57 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Mar 24 10:49:02 2011 Subject: [Histonet] RE: Microsatellite Instability Antibodies In-Reply-To: <1190CB05C44B13409483514729C2FC360C0B7B@PAIT42.olvg.nl> References: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA4F@MAIL-AG-CLUSTER.lha.org> <1190CB05C44B13409483514729C2FC360C0B7B@PAIT42.olvg.nl> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081F174880@CHEXCMS10.one.ads.che.org> We use Leica Bond Max and Biocare antibodies work very well for us. We're working on perfecting the Leica antibodies as well. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hoekert, W.E.J. Sent: Thursday, March 24, 2011 11:18 To: Evans, Andria B; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microsatellite Instability Antibodies >We are currently running the Ventana IHC stainers with the UltraView Detection. Just like us! MLH-1: Cell Marque (ref 760-4264). 30' cc1, 32' ab incubation. MSH-2: Cell Marque (ref 760-4265). 60' cc1, 60' ab inc. PMS2: Becton Dickinson, clone A16-4, cat nr 556415. 30' cc1, 32' ab inc. +amplification MSH6: Becton Dickinson, clone 44, cat nr 610919. 60' cc2, 60' ab inc. MSH-6 does not give very nice results, however, it works. Good Luck, Willem ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Evans, Andria B Verzonden: do 24-3-2011 13:31 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Microsatellite Instability Antibodies I am currently working up the MSI Antibodies (MLH1, MSH6, MSH2, and PMS2) and need some input on the protocol that you may be running in your lab and what clone(manufacture) you are using. We are currently running the Ventana IHC stainers with the UltraView Detection. Thanks for your help! Andria B Evans HTL(ASCP)CM This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From akemiat3377 <@t> yahoo.com Thu Mar 24 12:07:38 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Mar 24 12:07:43 2011 Subject: [Histonet] GREAT B5 substitute Message-ID: <465432.55346.qm@web113805.mail.gq1.yahoo.com> Hi All, I used to use B5, Zenker's and Helly's for years on LN and BM biopsies. I wanted to stop using mercury in our department, but didn't want to compromise the quality of the results we were getting. I was interested in using fixatives and decalcification products that would not effect IHC results. At the suggestion of Dr. Elaine Jaffe, Chief Hematopathologist from NIH, I tried 2 products from BBC Biochemical. I did parallel studies on bilateral BM bx's using B?Plus Fixative? and RapidCal?Immuno?, and then B5 fixative and EDTA decal solution, and then Zenker's fixative. We then did parallel studies for IHC. We ran a panel of Ab's on specimens which were treated with each of the solutions. The pathologists concluded that B?Plus Fixative? produces superb fixation for hematopoietic tissues and the RapidCal?Immuno? does not interfere with IHC staining. In some cases, the results on the IHC's were better on some of the Ab's using these products too. It only takes 2-3 hours of fixation time for BM biopsies. After prefixing, they can go directly into RapidCal?Immuno? for 45 minutes to 1 hour, washed in running water for 5 minutes, and then continue to be processed as normal starting off in 10% formalin. Just a note: This product is a rapid decalcifier designed to preserve immunogenicity at a maximum while accomplishing rapid decalcification. It is designed specifically for bone marrow biopsies and LN biopsies. By the way, I am not on BBC's payroll. I am always on a quest to improve patient care! Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com From dmlongoria <@t> ecrmc.org Thu Mar 24 12:00:57 2011 From: dmlongoria <@t> ecrmc.org (Diana Martinez-Longoria) Date: Thu Mar 24 12:09:15 2011 Subject: [Histonet] bone marrow biopsy decals Message-ID: Our pathologist wants to know why Formical-4 is the best decal for bone marrow biopsies, since we heard about in Histonet? I have experimented with four different decals: Rapid-Immuno, Formical-4, Nitric Acid, and HCL\Formic Acid and we are still not sure which will be the best decal. Also we a decal that will minimize the leeching out of iron. If any out there has any suggest, please let me know. Thanks in advance! Diana Martinez-Longoria Histotechnician HT(ASCP)CM El Centro Regional Medical Center 1415 Ross Ave El Centro CA 92243 (760) 339-7267 (760)482-5365 fax dmlongoria@ecrmc.org Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? From akemiat3377 <@t> yahoo.com Thu Mar 24 13:17:22 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Mar 24 13:17:46 2011 Subject: [Histonet] bone marrow biopsy decals In-Reply-To: References: Message-ID: <62D903C5-EF3C-4792-ABD2-962E5A72A602@yahoo.com> Hi Diana, I recently made a histonet posting "Great B5 substitute" which also addresses BM decal. Since there are a number of companies who sell decal solutions with similar names, I am not sure you tried the products from BBC Chemical. It is best to use both products together. My post goes into my findings. Regards, Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Mar 24, 2011, at 10:00 AM, Diana Martinez-Longoria wrote: > Our pathologist wants to know why Formical-4 is the best decal for > bone marrow biopsies, since we heard about in Histonet? I have > experimented with four different decals: Rapid-Immuno, Formical-4, > Nitric Acid, and HCL\Formic Acid and we are still not sure which > will be the best decal. Also we a decal that will minimize the > leeching out of iron. If any out there has any suggest, please let > me know. Thanks in advance! > > Diana Martinez-Longoria > Histotechnician HT(ASCP)CM > El Centro Regional Medical Center > 1415 Ross Ave > El Centro CA 92243 > (760) 339-7267 > (760)482-5365 fax > > dmlongoria@ecrmc.org > > > > > > > Confidentiality Notice: This e-mail is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or > distribution is prohibited. If you are not the intended recipient, > please contact the sender at the phone number above and promptly > destroy this e-mail and its attachments. > > > ECRMC Confidentiality Notice: This e-mail is for the sole use of > the intended recipient(s) and may contain confidential and > privileged information. Any unauthorized review, use, disclosure, > or distribution is prohibited. If you are not the intended > recipient, PLEASE contact the sender and promptly destroy this e- > mail and its attachments. > ? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Thu Mar 24 14:05:00 2011 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Mar 24 14:05:06 2011 Subject: [Histonet] RE: Stupid rabbit antibodies Message-ID: Have you tried the Dako Rabbit envision? We also tried the AbCam Expose Rabbit specific HRP and used Nova red from Vector or the DAB in the kit depending on which antibody we used. It is excellent , maybe even a little better than Dako's envision. I did have to use blocks such as FC receptor block Innovex, Powerblock Biogenex and serum free protein block DAKO. What I used depended on the antibody with some needing only the block provided in the kit. Good Luck. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ------------------------------ Message: 17 Date: Tue, 22 Mar 2011 16:04:16 -0500 From: Subject: [Histonet] Stupid Rabbit primaries! To: Message-ID: Content-Type: text/plain; charset="us-ascii" So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ------------------------------ Message: 18 Date: Tue, 22 Mar 2011 16:13:04 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] Stupid Rabbit primaries! To: , Message-ID: <8C023B4AB999614BA4791BAEB26E2738010029DE@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Do you know for sure that its the Ab and not endogenous biotin, peroxidase or Phosphatase? You can block for all those. Otherwise there are a number of commercial blocking agents on the market...try Biocare for a start (800)799-9499 or better yet, the company that makes your antibody. Good luck. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From akemiat3377 <@t> yahoo.com Thu Mar 24 15:03:40 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Mar 24 15:04:04 2011 Subject: [Histonet] GREAT B5 substitute In-Reply-To: <6DBD71C31D7E444482E5D3DFBC202D260324D64C@txhous1eb012.uson.usoncology.int> References: <465432.55346.qm@web113805.mail.gq1.yahoo.com> <6DBD71C31D7E444482E5D3DFBC202D260324D64C@txhous1eb012.uson.usoncology.int> Message-ID: <1FB5801D-95F0-437B-A884-E98491E1769E@yahoo.com> Having worked and developed products in the Biotech arena, not all products are disclosed. If they are, you lose the competitive edge. Here is the link to the the MSDS and information BCC discloses; http://www.bbcus.com/uploads/files/BPlusFix_MSDS.pdf Section 2. Composition and Information on Ingredients Name CAS# % by Weight Exposure Limits 1) Water 2) Formaldehyde 37% 3) Zinc Salts 4) Selected Buffers 7732-18-5 50-00-0 Not Available Not Available <25% >0.5% - <1% Not Available Not Available OSHA PEL 0.75ppm TWA Not Available Not Available Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Mar 24, 2011, at 12:28 PM, Johnson, Nacaela wrote: > Are the contents of the B Plus Fixative proprietary? Is it an AZF > type > of fixative? Did you try any ISH on the test runs? > > > Thanks, > > Nacaela Johnson, HTL (ASCP) > Histotechnologist > KCCC Pathology > 12000 110th St., Ste. 400 > Overland Park, KS 66210 > Office: 913-234-0576 > Fax: 913-433-7639 > Email: Nacaela.Johnson@USOncology.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi > Allison > Sent: Thursday, March 24, 2011 12:08 PM > To: histonet > Subject: [Histonet] GREAT B5 substitute > > Hi All, > I used to use B5, Zenker's and Helly's for years on LN and BM > biopsies. > I wanted to stop using mercury in our department, but didn't want to > compromise the quality of the results we were getting. > > I was interested in using fixatives and decalcification products that > would not effect IHC results. At the suggestion of Dr. Elaine Jaffe, > Chief Hematopathologist from NIH, I tried 2 products from BBC > Biochemical. I did parallel studies on bilateral BM bx's using B*Plus > Fixative(tm) and RapidCal*Immuno(tm), and then B5 fixative and EDTA > decal solution, and then Zenker's fixative. We then did parallel > studies for IHC. We ran a panel of Ab's on specimens which were > treated > with each of the solutions. > > The pathologists concluded that B*Plus Fixative(tm) produces superb > fixation for hematopoietic tissues and the RapidCal*Immuno(tm) does > not > interfere with IHC staining. In some cases, the results on the IHC's > were better on some of the Ab's using these products too. > > It only takes 2-3 hours of fixation time for BM biopsies. After > prefixing, they can go directly into RapidCal*Immuno(tm) for 45 > minutes > to 1 hour, washed in running water for 5 minutes, and then continue to > be processed as normal starting off in 10% formalin. Just a note: > This > product is a rapid decalcifier designed to preserve immunogenicity > at a > maximum while accomplishing rapid decalcification. It is designed > specifically for bone marrow biopsies and LN biopsies. > > By the way, I am not on BBC's payroll. I am always on a quest to > improve patient care! > > Akemi Allison BS, HT(ASCP)HTL > Director > Phoenix Lab Consulting > E-Mail: akemiat3377@yahoo.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > The contents of this electronic mail message and any > attachments are confidential, possibly privileged and intended for > the addressee(s) only.
Only the addressee(s) may read, > disseminate, retain or otherwise use this message. If received in > error, please immediately inform the sender and then delete this > message without disclosing its contents to anyone. > From alisha <@t> ka-recruiting.com Thu Mar 24 16:51:45 2011 From: alisha <@t> ka-recruiting.com (Alisha Dynan) Date: Thu Mar 24 16:51:48 2011 Subject: [Histonet] Great Pathology Laboratory Manager position in NC Message-ID: <825205274.1301003505037.JavaMail.cfservice@SL4APP4> Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. Below are some Histotechnologist positions we are currently working on: Highlighted Job Opportunity: Pathology Laboratory Manager in Western NC I am currently working with a large hospital system in Western NC that is looking to hire a Pathology Laboratory Manager. This non-profit independent community hospital is the busiest surgical hospital in the state. My client is looking for an experienced histology professionals with technical expertise in all aspects of histology, IHC, coding/billing. Experience with CoPath is a plus. My client is looking for someone who is HT or HTL(ASCP) certified and has at least 2 years of management experience at a medium to large sized hospital. This area of NC has been recognized as one of the top travel destinations in the world and one of the top 10 places to live in the United States. My client provides an excellent compensation package including a competitive salary, relocation assistance, and full benefits.If interested in learning more, please email me at alisha@ka-recruiting.com. If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com From sukey <@t> compassdermpath.com Thu Mar 24 17:44:46 2011 From: sukey <@t> compassdermpath.com (=?utf-8?b?U3VzYW4gQS4gS2VsbHk=?=) Date: Thu Mar 24 17:44:51 2011 Subject: [Histonet] part time/ per diem Job opportunity in San Diego, CA Message-ID: <20110324184446.79wfuds5naosoc8w@webmail.compassdermpath.com> Hello, Fellow Histonetters; ? I am currently in search of an ASCP registered Histotechnician to cover vacations?in our new Dermatopathology laboratory.? The hours are flexible?and the current?need for?coverage?will be the last 2 weeks in May.? ? There would be an?overlap of 3-4 days to orient?you to?the daily schedule.???There is great potential for the position to work into?a full time.???? ? The lab is fully equipped with top of the line products.? Daily procedures include processing, embedding, sectioning, automated H&E staining, special stains and automated Immunohistochemistry?as needed.??We also perform cryosectioning and?one-step Immunofluorescence staining (set up to run on the automated IHC stainer).? ? Please contact me if you have any additional questions or are interested in the position.??Our?two dermatopathologist are a pleasure to work with. ? I look forward to hearing from interested parties.? ? Kind Regards, ? Susan _____________________________________ Susan A. Kelly, H.T., H.T.L.(ASCP)QIHC Lab Manager Compass Dermatopathology, Inc. 7300 Girard Ave, Suite 104 LaJolla, CA 92037 Phone: (858)750-2983 Fax: (858)750-2984 sukey@compassdermpath.com ? From amitapandey <@t> torrentpharma.com Thu Mar 24 23:26:52 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Thu Mar 24 23:30:37 2011 Subject: [Histonet] Any body interested to sell the microscope ? In-Reply-To: <1190CB05C44B13409483514729C2FC360C0B7B@PAIT42.olvg.nl> References: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA4F@MAIL-AG-CLUSTER.lha.org> <1190CB05C44B13409483514729C2FC360C0B7B@PAIT42.olvg.nl> Message-ID: Dear Histonet members, I am out in market to buy a upright light microscope. (I am looking for lower to medium end microscope- Nikon 50i/ Zeiss Axiolab etc.) Any body interested to sell their old but good working microscope , please contact me. Additionally If it has camera attach or image analysis software, it will be benefit to me but not the essential one. Amita From pruegg <@t> ihctech.net Fri Mar 25 10:22:11 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Mar 25 10:22:22 2011 Subject: [Histonet] bone marrow biopsy decals In-Reply-To: References: Message-ID: Diana, I have a lot of experience with decal reagents and Formic acid is the best in my experience for IHC. It is not as fast as the rapid decals but is the kindest at not adversely affecting the antigens and more gentle on iron leaching, although all decals will leach iron, we usually do iron on the smear or clot section. Not only does the formic acid leach the iron so does the alcohols and solvents in tissue processing. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana Martinez-Longoria Sent: Thursday, March 24, 2011 11:01 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] bone marrow biopsy decals Our pathologist wants to know why Formical-4 is the best decal for bone marrow biopsies, since we heard about in Histonet? I have experimented with four different decals: Rapid-Immuno, Formical-4, Nitric Acid, and HCL\Formic Acid and we are still not sure which will be the best decal. Also we a decal that will minimize the leeching out of iron. If any out there has any suggest, please let me know. Thanks in advance! Diana Martinez-Longoria Histotechnician HT(ASCP)CM El Centro Regional Medical Center 1415 Ross Ave El Centro CA 92243 (760) 339-7267 (760)482-5365 fax dmlongoria@ecrmc.org Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Mar 25 10:25:16 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Mar 25 10:25:25 2011 Subject: [Histonet] RE: Microsatellite Instability Antibodies In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164081F174880@CHEXCMS10.one.ads.che.org> References: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA4F@MAIL-AG-CLUSTER.lha.org><1190CB05C44B13409483514729C2FC360C0B7B@PAIT42.olvg.nl> <92AD9B20A6C38C4587A9FEBE3A30E164081F174880@CHEXCMS10.one.ads.che.org> Message-ID: <40B8402295F54422B2C4638E76323F1A@prueggihctechlt> I use the NovaCastra MSH-2 and MLH-1 on the Leica Bond Maxx. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, March 24, 2011 9:49 AM To: Hoekert, W.E.J.; Evans, Andria B; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Microsatellite Instability Antibodies We use Leica Bond Max and Biocare antibodies work very well for us. We're working on perfecting the Leica antibodies as well. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hoekert, W.E.J. Sent: Thursday, March 24, 2011 11:18 To: Evans, Andria B; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microsatellite Instability Antibodies >We are currently running the Ventana IHC stainers with the UltraView Detection. Just like us! MLH-1: Cell Marque (ref 760-4264). 30' cc1, 32' ab incubation. MSH-2: Cell Marque (ref 760-4265). 60' cc1, 60' ab inc. PMS2: Becton Dickinson, clone A16-4, cat nr 556415. 30' cc1, 32' ab inc. +amplification MSH6: Becton Dickinson, clone 44, cat nr 610919. 60' cc2, 60' ab inc. MSH-6 does not give very nice results, however, it works. Good Luck, Willem ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Evans, Andria B Verzonden: do 24-3-2011 13:31 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Microsatellite Instability Antibodies I am currently working up the MSI Antibodies (MLH1, MSH6, MSH2, and PMS2) and need some input on the protocol that you may be running in your lab and what clone(manufacture) you are using. We are currently running the Ventana IHC stainers with the UltraView Detection. Thanks for your help! Andria B Evans HTL(ASCP)CM This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From atyler <@t> pcg.wustl.edu Fri Mar 25 11:06:00 2011 From: atyler <@t> pcg.wustl.edu (Amber Tyler) Date: Fri Mar 25 11:06:45 2011 Subject: [Histonet] Vaginal Smears-Fixation Message-ID: Good Morning, I am doing a study on changes in estrous cycling in mice over the long term. I will be collecting vaginal smears daily and staining with new methylene blue. I need to be able to keep the slides "fresh" for viewing for approximately a week. Does anyone have any advice on the best manner of fixing/mounting procedures for this? I have little experience with this. In previous studies, the slides were only needed briefly and discarded. Thank you, Amber -- Amber N. Tyler, Ph.D. Staff Scientist Anatomy & Neurobiology From Marcia_Gaiser <@t> ssmhc.com Fri Mar 25 11:29:21 2011 From: Marcia_Gaiser <@t> ssmhc.com (Gaiser, Marcia) Date: Fri Mar 25 11:29:28 2011 Subject: [Histonet] (no subject) Message-ID: <728F817C02110E498D803A7C3B0C6248068B1F5B38@S009-APEXM06.ds.ad.ssmhc.com> St. Anthony Hospital currently has an excellent opportunity for an experienced Histologic Technician. This position requires Certification as an HT or HLT ? or ? experience acceptable to the Laboratory Director. Two years of previous histology experience required with IHC and/or grossing experience a plus. Outstanding benefits package, including generous paid time off. For consideration, please apply online at www.saintsok.com>>>, Ad # 10762, or contact Anna King for additional information at (405) 272-6105. Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ From pruegg <@t> ihctech.net Fri Mar 25 11:51:01 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Mar 25 11:51:11 2011 Subject: [Histonet] RE: Stupid rabbit antibodies In-Reply-To: References: Message-ID: <41519003ECD14D6A9E27647AD10208D0@prueggihctechlt> Are you using the rab abs on human samples or another species? For most purposes serum free protein block should be all you need. If you were using rab ab on rabbits I would recommend the FC block from Innogenex, you can also get a anti rab link that is Fab2 and perhaps absorbed to the species you are working with. Southern Biotech sells really good secondary link reagents, u can get it hrp, ap, fitc, or unlabeled and then match up the detection to the animal the link is made in. are you using a avidin/biotin detection system? If you are there may be endogenous biotin which can be blocked. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Thursday, March 24, 2011 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Stupid rabbit antibodies Have you tried the Dako Rabbit envision? We also tried the AbCam Expose Rabbit specific HRP and used Nova red from Vector or the DAB in the kit depending on which antibody we used. It is excellent , maybe even a little better than Dako's envision. I did have to use blocks such as FC receptor block Innovex, Powerblock Biogenex and serum free protein block DAKO. What I used depended on the antibody with some needing only the block provided in the kit. Good Luck. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ------------------------------ Message: 17 Date: Tue, 22 Mar 2011 16:04:16 -0500 From: Subject: [Histonet] Stupid Rabbit primaries! To: Message-ID: Content-Type: text/plain; charset="us-ascii" So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ------------------------------ Message: 18 Date: Tue, 22 Mar 2011 16:13:04 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] Stupid Rabbit primaries! To: , Message-ID: <8C023B4AB999614BA4791BAEB26E2738010029DE@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Do you know for sure that its the Ab and not endogenous biotin, peroxidase or Phosphatase? You can block for all those. Otherwise there are a number of commercial blocking agents on the market...try Biocare for a start (800)799-9499 or better yet, the company that makes your antibody. Good luck. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From immrstambo <@t> hotmail.com Fri Mar 25 12:44:55 2011 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Fri Mar 25 12:45:00 2011 Subject: [Histonet] looking for job opening for histotech in South Carolina Message-ID: Hello histonetters! I hope everyone has a great weekend. I am helping a friend who is relocating to South Carolina. Does anyone know of any job openings in that area? She is looking for something in Greenville, SC or Baltimore, MD. Any tips would be appreciated. You can email me back or post on the net.... Thanks again to all my fellow histonetters!! Christine Tambasco, HT (ASCP) Albany Medical Center Hospital Albany, NY From broyce <@t> NSH.org Fri Mar 25 13:59:22 2011 From: broyce <@t> NSH.org (Brenda Royce) Date: Fri Mar 25 13:59:31 2011 Subject: [Histonet] RE: Histonet Digest, Vol 88, Issue 33 In-Reply-To: <43a92309-7770-4dd2-957e-cb8367386add@NSH-SRVR01.nsh.local> References: <43a92309-7770-4dd2-957e-cb8367386add@NSH-SRVR01.nsh.local> Message-ID: <9499F24DDCCCF6479DA910DE3D5AA7FC0FDDFE@NSH-SRVR01.nsh.local> To all NSH Members - Don't forget to submit your contest entry form on how your lab celebrated HPD! Due by March 31, 2011 for chance to win free education for the entire lab. Visit http://www.nsh.org/content/histotechnology-day for more information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, March 25, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 88, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. GREAT B5 substitute (Akemi Allison) 2. bone marrow biopsy decals (Diana Martinez-Longoria) 3. Re: bone marrow biopsy decals (Akemi Allison) 4. RE: Stupid rabbit antibodies (Perry, Margaret) 5. Re: GREAT B5 substitute (Akemi Allison) 6. Great Pathology Laboratory Manager position in NC (Alisha Dynan) 7. part time/ per diem Job opportunity in San Diego, CA ( Susan A. Kelly ) 8. Any body interested to sell the microscope ? (amitapandey@torrentpharma.com) 9. RE: bone marrow biopsy decals (Patsy Ruegg) 10. RE: RE: Microsatellite Instability Antibodies (Patsy Ruegg) 11. Vaginal Smears-Fixation (Amber Tyler) 12. (no subject) (Gaiser, Marcia) 13. RE: RE: Stupid rabbit antibodies (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Thu, 24 Mar 2011 10:07:38 -0700 (PDT) From: Akemi Allison Subject: [Histonet] GREAT B5 substitute To: histonet Message-ID: <465432.55346.qm@web113805.mail.gq1.yahoo.com> Content-Type: text/plain; charset=utf-8 Hi All, I used to use B5, Zenker's and Helly's for years on LN and BM biopsies. I wanted to stop using mercury in our department, but didn't want to compromise the quality of the results we were getting. I was interested in using fixatives and decalcification products that would not effect IHC results. At the suggestion of Dr. Elaine Jaffe, Chief Hematopathologist from NIH, I tried 2 products from BBC Biochemical. I did parallel studies on bilateral BM bx's using B?Plus Fixative? and RapidCal?Immuno?, and then B5 fixative and EDTA decal solution, and then Zenker's fixative. We then did parallel studies for IHC. We ran a panel of Ab's on specimens which were treated with each of the solutions. The pathologists concluded that B?Plus Fixative? produces superb fixation for hematopoietic tissues and the RapidCal?Immuno? does not interfere with IHC staining. In some cases, the results on the IHC's were better on some of the Ab's using these products too. It only takes 2-3 hours of fixation time for BM biopsies. After prefixing, they can go directly into RapidCal?Immuno? for 45 minutes to 1 hour, washed in running water for 5 minutes, and then continue to be processed as normal starting off in 10% formalin. Just a note: This product is a rapid decalcifier designed to preserve immunogenicity at a maximum while accomplishing rapid decalcification. It is designed specifically for bone marrow biopsies and LN biopsies. By the way, I am not on BBC's payroll. I am always on a quest to improve patient care! Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ------------------------------ Message: 2 Date: Thu, 24 Mar 2011 10:00:57 -0700 From: Diana Martinez-Longoria Subject: [Histonet] bone marrow biopsy decals To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Our pathologist wants to know why Formical-4 is the best decal for bone marrow biopsies, since we heard about in Histonet? I have experimented with four different decals: Rapid-Immuno, Formical-4, Nitric Acid, and HCL\Formic Acid and we are still not sure which will be the best decal. Also we a decal that will minimize the leeching out of iron. If any out there has any suggest, please let me know. Thanks in advance! Diana Martinez-Longoria Histotechnician HT(ASCP)CM El Centro Regional Medical Center 1415 Ross Ave El Centro CA 92243 (760) 339-7267 (760)482-5365 fax dmlongoria@ecrmc.org Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ? ------------------------------ Message: 3 Date: Thu, 24 Mar 2011 11:17:22 -0700 From: Akemi Allison Subject: Re: [Histonet] bone marrow biopsy decals To: Diana Martinez-Longoria Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: <62D903C5-EF3C-4792-ABD2-962E5A72A602@yahoo.com> Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed Hi Diana, I recently made a histonet posting "Great B5 substitute" which also addresses BM decal. Since there are a number of companies who sell decal solutions with similar names, I am not sure you tried the products from BBC Chemical. It is best to use both products together. My post goes into my findings. Regards, Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Mar 24, 2011, at 10:00 AM, Diana Martinez-Longoria wrote: > Our pathologist wants to know why Formical-4 is the best decal for > bone marrow biopsies, since we heard about in Histonet? I have > experimented with four different decals: Rapid-Immuno, Formical-4, > Nitric Acid, and HCL\Formic Acid and we are still not sure which will > be the best decal. Also we a decal that will minimize the leeching > out of iron. If any out there has any suggest, please let me know. > Thanks in advance! > > Diana Martinez-Longoria > Histotechnician HT(ASCP)CM > El Centro Regional Medical Center > 1415 Ross Ave > El Centro CA 92243 > (760) 339-7267 > (760)482-5365 fax > > dmlongoria@ecrmc.org > > > > > > > Confidentiality Notice: This e-mail is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution > is prohibited. If you are not the intended recipient, please contact > the sender at the phone number above and promptly destroy this e-mail > and its attachments. > > > ECRMC Confidentiality Notice: This e-mail is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution > is prohibited. If you are not the intended recipient, PLEASE contact > the sender and promptly destroy this e- mail and its attachments. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 24 Mar 2011 14:05:00 -0500 From: "Perry, Margaret" Subject: [Histonet] RE: Stupid rabbit antibodies To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Have you tried the Dako Rabbit envision? We also tried the AbCam Expose Rabbit specific HRP and used Nova red from Vector or the DAB in the kit depending on which antibody we used. It is excellent , maybe even a little better than Dako's envision. I did have to use blocks such as FC receptor block Innovex, Powerblock Biogenex and serum free protein block DAKO. What I used depended on the antibody with some needing only the block provided in the kit. Good Luck. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ------------------------------ Message: 17 Date: Tue, 22 Mar 2011 16:04:16 -0500 From: Subject: [Histonet] Stupid Rabbit primaries! To: Message-ID: Content-Type: text/plain; charset="us-ascii" So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ------------------------------ Message: 18 Date: Tue, 22 Mar 2011 16:13:04 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] Stupid Rabbit primaries! To: , Message-ID: <8C023B4AB999614BA4791BAEB26E2738010029DE@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Do you know for sure that its the Ab and not endogenous biotin, peroxidase or Phosphatase? You can block for all those. Otherwise there are a number of commercial blocking agents on the market...try Biocare for a start (800)799-9499 or better yet, the company that makes your antibody. Good luck. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ------------------------------ Message: 5 Date: Thu, 24 Mar 2011 13:03:40 -0700 From: Akemi Allison Subject: Re: [Histonet] GREAT B5 substitute To: "Johnson, Nacaela" Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: <1FB5801D-95F0-437B-A884-E98491E1769E@yahoo.com> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Having worked and developed products in the Biotech arena, not all products are disclosed. If they are, you lose the competitive edge. Here is the link to the the MSDS and information BCC discloses; http://www.bbcus.com/uploads/files/BPlusFix_MSDS.pdf Section 2. Composition and Information on Ingredients Name CAS# % by Weight Exposure Limits 1) Water 2) Formaldehyde 37% 3) Zinc Salts 4) Selected Buffers 7732-18-5 50-00-0 Not Available Not Available <25% >0.5% - <1% Not Available Not Available OSHA PEL 0.75ppm TWA Not Available Not Available Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Mar 24, 2011, at 12:28 PM, Johnson, Nacaela wrote: > Are the contents of the B Plus Fixative proprietary? Is it an AZF > type > of fixative? Did you try any ISH on the test runs? > > > Thanks, > > Nacaela Johnson, HTL (ASCP) > Histotechnologist > KCCC Pathology > 12000 110th St., Ste. 400 > Overland Park, KS 66210 > Office: 913-234-0576 > Fax: 913-433-7639 > Email: Nacaela.Johnson@USOncology.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi > Allison > Sent: Thursday, March 24, 2011 12:08 PM > To: histonet > Subject: [Histonet] GREAT B5 substitute > > Hi All, > I used to use B5, Zenker's and Helly's for years on LN and BM > biopsies. > I wanted to stop using mercury in our department, but didn't want to > compromise the quality of the results we were getting. > > I was interested in using fixatives and decalcification products that > would not effect IHC results. At the suggestion of Dr. Elaine Jaffe, > Chief Hematopathologist from NIH, I tried 2 products from BBC > Biochemical. I did parallel studies on bilateral BM bx's using B*Plus > Fixative(tm) and RapidCal*Immuno(tm), and then B5 fixative and EDTA > decal solution, and then Zenker's fixative. We then did parallel > studies for IHC. We ran a panel of Ab's on specimens which were > treated > with each of the solutions. > > The pathologists concluded that B*Plus Fixative(tm) produces superb > fixation for hematopoietic tissues and the RapidCal*Immuno(tm) does > not > interfere with IHC staining. In some cases, the results on the IHC's > were better on some of the Ab's using these products too. > > It only takes 2-3 hours of fixation time for BM biopsies. After > prefixing, they can go directly into RapidCal*Immuno(tm) for 45 > minutes > to 1 hour, washed in running water for 5 minutes, and then continue to > be processed as normal starting off in 10% formalin. Just a note: > This > product is a rapid decalcifier designed to preserve immunogenicity > at a > maximum while accomplishing rapid decalcification. It is designed > specifically for bone marrow biopsies and LN biopsies. > > By the way, I am not on BBC's payroll. I am always on a quest to > improve patient care! > > Akemi Allison BS, HT(ASCP)HTL > Director > Phoenix Lab Consulting > E-Mail: akemiat3377@yahoo.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > The contents of this electronic mail message and any > attachments are confidential, possibly privileged and intended for > the addressee(s) only.
Only the addressee(s) may read, > disseminate, retain or otherwise use this message. If received in > error, please immediately inform the sender and then delete this > message without disclosing its contents to anyone. > ------------------------------ Message: 6 Date: 24 Mar 2011 17:51:45 -0400 From: Alisha Dynan Subject: [Histonet] Great Pathology Laboratory Manager position in NC To: histonet@lists.utsouthwestern.edu Message-ID: <825205274.1301003505037.JavaMail.cfservice@SL4APP4> Content-Type: text/plain; charset="utf-8" Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. Below are some Histotechnologist positions we are currently working on: Highlighted Job Opportunity: Pathology Laboratory Manager in Western NC I am currently working with a large hospital system in Western NC that is looking to hire a Pathology Laboratory Manager. This non-profit independent community hospital is the busiest surgical hospital in the state. My client is looking for an experienced histology professionals with technical expertise in all aspects of histology, IHC, coding/billing. Experience with CoPath is a plus. My client is looking for someone who is HT or HTL(ASCP) certified and has at least 2 years of management experience at a medium to large sized hospital. This area of NC has been recognized as one of the top travel destinations in the world and one of the top 10 places to live in the United States. My client provides an excellent compensation package including a competitive salary, relocation assistance, and full benefits.If interested in learning more, please email me at alisha@ka-recruiting.com. If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 alisha@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 7 Date: Thu, 24 Mar 2011 18:44:46 -0400 From: " Susan A. Kelly " Subject: [Histonet] part time/ per diem Job opportunity in San Diego, CA To: Histonet@lists.utsouthwestern.edu Message-ID: <20110324184446.79wfuds5naosoc8w@webmail.compassdermpath.com> Content-Type: text/plain; charset=UTF-8 Hello, Fellow Histonetters; ? I am currently in search of an ASCP registered Histotechnician to cover vacations?in our new Dermatopathology laboratory.? The hours are flexible?and the current?need for?coverage?will be the last 2 weeks in May.? ? There would be an?overlap of 3-4 days to orient?you to?the daily schedule.???There is great potential for the position to work into?a full time.???? ? The lab is fully equipped with top of the line products.? Daily procedures include processing, embedding, sectioning, automated H&E staining, special stains and automated Immunohistochemistry?as needed.??We also perform cryosectioning and?one-step Immunofluorescence staining (set up to run on the automated IHC stainer).? ? Please contact me if you have any additional questions or are interested in the position.??Our?two dermatopathologist are a pleasure to work with. ? I look forward to hearing from interested parties.? ? Kind Regards, ? Susan _____________________________________ Susan A. Kelly, H.T., H.T.L.(ASCP)QIHC Lab Manager Compass Dermatopathology, Inc. 7300 Girard Ave, Suite 104 LaJolla, CA 92037 Phone: (858)750-2983 Fax: (858)750-2984 sukey@compassdermpath.com ? ------------------------------ Message: 8 Date: Fri, 25 Mar 2011 09:56:52 +0530 From: amitapandey@torrentpharma.com Subject: [Histonet] Any body interested to sell the microscope ? To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Dear Histonet members, I am out in market to buy a upright light microscope. (I am looking for lower to medium end microscope- Nikon 50i/ Zeiss Axiolab etc.) Any body interested to sell their old but good working microscope , please contact me. Additionally If it has camera attach or image analysis software, it will be benefit to me but not the essential one. Amita ------------------------------ Message: 9 Date: Fri, 25 Mar 2011 09:22:11 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] bone marrow biopsy decals To: "'Diana Martinez-Longoria'" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Diana, I have a lot of experience with decal reagents and Formic acid is the best in my experience for IHC. It is not as fast as the rapid decals but is the kindest at not adversely affecting the antigens and more gentle on iron leaching, although all decals will leach iron, we usually do iron on the smear or clot section. Not only does the formic acid leach the iron so does the alcohols and solvents in tissue processing. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana Martinez-Longoria Sent: Thursday, March 24, 2011 11:01 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] bone marrow biopsy decals Our pathologist wants to know why Formical-4 is the best decal for bone marrow biopsies, since we heard about in Histonet? I have experimented with four different decals: Rapid-Immuno, Formical-4, Nitric Acid, and HCL\Formic Acid and we are still not sure which will be the best decal. Also we a decal that will minimize the leeching out of iron. If any out there has any suggest, please let me know. Thanks in advance! Diana Martinez-Longoria Histotechnician HT(ASCP)CM El Centro Regional Medical Center 1415 Ross Ave El Centro CA 92243 (760) 339-7267 (760)482-5365 fax dmlongoria@ecrmc.org Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 25 Mar 2011 09:25:16 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] RE: Microsatellite Instability Antibodies To: "'Weems, Joyce'" , "'Hoekert, W.E.J.'" , "'Evans, Andria B'" , Message-ID: <40B8402295F54422B2C4638E76323F1A@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" I use the NovaCastra MSH-2 and MLH-1 on the Leica Bond Maxx. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, March 24, 2011 9:49 AM To: Hoekert, W.E.J.; Evans, Andria B; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Microsatellite Instability Antibodies We use Leica Bond Max and Biocare antibodies work very well for us. We're working on perfecting the Leica antibodies as well. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hoekert, W.E.J. Sent: Thursday, March 24, 2011 11:18 To: Evans, Andria B; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microsatellite Instability Antibodies >We are currently running the Ventana IHC stainers with the UltraView Detection. Just like us! MLH-1: Cell Marque (ref 760-4264). 30' cc1, 32' ab incubation. MSH-2: Cell Marque (ref 760-4265). 60' cc1, 60' ab inc. PMS2: Becton Dickinson, clone A16-4, cat nr 556415. 30' cc1, 32' ab inc. +amplification MSH6: Becton Dickinson, clone 44, cat nr 610919. 60' cc2, 60' ab inc. MSH-6 does not give very nice results, however, it works. Good Luck, Willem ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Evans, Andria B Verzonden: do 24-3-2011 13:31 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Microsatellite Instability Antibodies I am currently working up the MSI Antibodies (MLH1, MSH6, MSH2, and PMS2) and need some input on the protocol that you may be running in your lab and what clone(manufacture) you are using. We are currently running the Ventana IHC stainers with the UltraView Detection. Thanks for your help! Andria B Evans HTL(ASCP)CM This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 25 Mar 2011 11:06:00 -0500 From: Amber Tyler Subject: [Histonet] Vaginal Smears-Fixation To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Good Morning, I am doing a study on changes in estrous cycling in mice over the long term. I will be collecting vaginal smears daily and staining with new methylene blue. I need to be able to keep the slides "fresh" for viewing for approximately a week. Does anyone have any advice on the best manner of fixing/mounting procedures for this? I have little experience with this. In previous studies, the slides were only needed briefly and discarded. Thank you, Amber -- Amber N. Tyler, Ph.D. Staff Scientist Anatomy & Neurobiology ------------------------------ Message: 12 Date: Fri, 25 Mar 2011 11:29:21 -0500 From: "Gaiser, Marcia" Subject: [Histonet] (no subject) To: "histonet@lists.utsouthwestern.edu" Message-ID: <728F817C02110E498D803A7C3B0C6248068B1F5B38@S009-APEXM06.ds.ad.ssmhc.com> Content-Type: text/plain; charset="Windows-1252" St. Anthony Hospital currently has an excellent opportunity for an experienced Histologic Technician. This position requires Certification as an HT or HLT or experience acceptable to the Laboratory Director. Two years of previous histology experience required with IHC and/or grossing experience a plus. Outstanding benefits package, including generous paid time off. For consideration, please apply online at www.saintsok.com>>>, Ad # 10762, or contact Anna King for additional information at (405) 272-6105. Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ ------------------------------ Message: 13 Date: Fri, 25 Mar 2011 10:51:01 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] RE: Stupid rabbit antibodies To: "'Perry, Margaret'" , Message-ID: <41519003ECD14D6A9E27647AD10208D0@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" Are you using the rab abs on human samples or another species? For most purposes serum free protein block should be all you need. If you were using rab ab on rabbits I would recommend the FC block from Innogenex, you can also get a anti rab link that is Fab2 and perhaps absorbed to the species you are working with. Southern Biotech sells really good secondary link reagents, u can get it hrp, ap, fitc, or unlabeled and then match up the detection to the animal the link is made in. are you using a avidin/biotin detection system? If you are there may be endogenous biotin which can be blocked. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Thursday, March 24, 2011 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Stupid rabbit antibodies Have you tried the Dako Rabbit envision? We also tried the AbCam Expose Rabbit specific HRP and used Nova red from Vector or the DAB in the kit depending on which antibody we used. It is excellent , maybe even a little better than Dako's envision. I did have to use blocks such as FC receptor block Innovex, Powerblock Biogenex and serum free protein block DAKO. What I used depended on the antibody with some needing only the block provided in the kit. Good Luck. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ------------------------------ Message: 17 Date: Tue, 22 Mar 2011 16:04:16 -0500 From: Subject: [Histonet] Stupid Rabbit primaries! To: Message-ID: Content-Type: text/plain; charset="us-ascii" So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ------------------------------ Message: 18 Date: Tue, 22 Mar 2011 16:13:04 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] Stupid Rabbit primaries! To: , Message-ID: <8C023B4AB999614BA4791BAEB26E2738010029DE@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Do you know for sure that its the Ab and not endogenous biotin, peroxidase or Phosphatase? You can block for all those. Otherwise there are a number of commercial blocking agents on the market...try Biocare for a start (800)799-9499 or better yet, the company that makes your antibody. Good luck. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 88, Issue 33 **************************************** From immrstambo <@t> hotmail.com Sat Mar 26 05:36:43 2011 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Sat Mar 26 05:36:46 2011 Subject: [Histonet] Thanks for all the leads Message-ID: Thanks for all the leads, please continue to send them as you hear about them. You guys are great! I will forward to my histo friend in need. Christine Tambasco Albany Medical Center Albany,NY From Rcartun <@t> harthosp.org Sun Mar 27 11:52:48 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Mar 27 11:52:58 2011 Subject: [Histonet] IDH1 R132H IHC Message-ID: <4D8F331F.7400.0077.1@harthosp.org> Is anyone doing IHC for the IDH1 R132H point mutation for astrocytoma and oligodendroglioma in formalin-fixed, paraffin-embedded brain tissue using the H09 clone? If so, how do you get the antibody? Directly from Germany or is there someone here in the USA that carries it? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From ROrr <@t> northshore.org Mon Mar 28 07:11:08 2011 From: ROrr <@t> northshore.org (Orr, Rebecca) Date: Mon Mar 28 07:11:13 2011 Subject: [Histonet] RE: IDH1 R132H IHC In-Reply-To: <7180b51a-f145-412b-9ef9-0071c4daf254@EXCHCAS02.enhnet.org> References: <7180b51a-f145-412b-9ef9-0071c4daf254@EXCHCAS02.enhnet.org> Message-ID: Rich, The latest I have is from an order I placed for this product in 2010. It may have changed. My catalog number is DIA H09L US Rep Kitty Stein k.stein.dianova@gmail.com phone 786-343-2692 Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 Message: 1 Date: Sun, 27 Mar 2011 12:52:48 -0400 From: "Richard Cartun" Subject: [Histonet] IDH1 R132H IHC To: "Histonet" Message-ID: <4D8F331F.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII Is anyone doing IHC for the IDH1 R132H point mutation for astrocytoma and oligodendroglioma in formalin-fixed, paraffin-embedded brain tissue using the H09 clone? If so, how do you get the antibody? Directly from Germany or is there someone here in the USA that carries it? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. From JWatson <@t> gnf.org Mon Mar 28 10:44:22 2011 From: JWatson <@t> gnf.org (James Watson) Date: Mon Mar 28 10:44:25 2011 Subject: [Histonet] RE: IDH1 R132H IHC In-Reply-To: References: <7180b51a-f145-412b-9ef9-0071c4daf254@EXCHCAS02.enhnet.org> Message-ID: Our last order to dianova was switched to their new US distributor: http://www.histobiotec.com/home.php?cat= check with them James Watson HT? ASCP Facilities Manager of Histology GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: Monday, March 28, 2011 5:11 AM To: 'histonet@lists.utsouthwestern.edu' Cc: 'Rcartun@harthosp.org' Subject: [Histonet] RE: IDH1 R132H IHC Rich, The latest I have is from an order I placed for this product in 2010. It may have changed. My catalog number is DIA H09L US Rep Kitty Stein k.stein.dianova@gmail.com phone 786-343-2692 Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 Message: 1 Date: Sun, 27 Mar 2011 12:52:48 -0400 From: "Richard Cartun" Subject: [Histonet] IDH1 R132H IHC To: "Histonet" Message-ID: <4D8F331F.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII Is anyone doing IHC for the IDH1 R132H point mutation for astrocytoma and oligodendroglioma in formalin-fixed, paraffin-embedded brain tissue using the H09 clone? If so, how do you get the antibody? Directly from Germany or is there someone here in the USA that carries it? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jgoldsmi <@t> bidmc.harvard.edu Mon Mar 28 12:04:10 2011 From: jgoldsmi <@t> bidmc.harvard.edu (jgoldsmi@bidmc.harvard.edu) Date: Mon Mar 28 12:04:15 2011 Subject: [Histonet] IDH1 In-Reply-To: <201103271701.p2RH0vSf023032@sr48molokai.caregroup.org> References: <201103271701.p2RH0vSf023032@sr48molokai.caregroup.org> Message-ID: Hey Richard, I know that BWH is running it; you might want to contact Jason Hornick there for his conditions. Jeff Jeffrey Goldsmith, MD Staff Pathologist Beth Israel Deaconess Medical Center Children's Hospital Boston 330 Brookline Avenue Boston, MA 02215 BIDMC Office: 617 667 2586 BIDMC Fax: 617 975 5620 CHB Pathology Department (for CHB adminstrative matters only): 617 355 7431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, March 27, 2011 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 88, Issue 35 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IDH1 R132H IHC (Richard Cartun) ---------------------------------------------------------------------- Message: 1 Date: Sun, 27 Mar 2011 12:52:48 -0400 From: "Richard Cartun" Subject: [Histonet] IDH1 R132H IHC To: "Histonet" Message-ID: <4D8F331F.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII Is anyone doing IHC for the IDH1 R132H point mutation for astrocytoma and oligodendroglioma in formalin-fixed, paraffin-embedded brain tissue using the H09 clone? If so, how do you get the antibody? Directly from Germany or is there someone here in the USA that carries it? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 88, Issue 35 **************************************** From Karla.Sendelbach <@t> thedacare.org Mon Mar 28 12:44:02 2011 From: Karla.Sendelbach <@t> thedacare.org (Karla Sendelbach) Date: Mon Mar 28 12:44:08 2011 Subject: [Histonet] to microwave or not to microwave Message-ID: We are evaluating rapid tissue processing instruments. I would appreciate any comments about microwave tissue process and rapid non microwave tissue process. Peloris II, STP 420ES and Milestone Pathos come to mind. Also, is there anyone who switched from microwave processing to rapid non microwave tissue processing? Thank you, Dr. Sendelbach Appleton Medical Center Appleton Wisconsin 920.738.6294 From kkmarshall <@t> anthc.org Mon Mar 28 15:32:32 2011 From: kkmarshall <@t> anthc.org (Marshall, Kimberly K) Date: Mon Mar 28 15:32:44 2011 Subject: [Histonet] Biopsy Run Message-ID: Hello out there!!! Just a couple of quick questions...I wrote before with a "artifact" problem and I thank everyone for their input. Bad thing is, I am still having issues. Would my fellow Histo Techs please share with me the times you use on your Biopsy Run's as well maybe give me some advise on biopsy cassettes. I have used 2 different kind and just cant help but wonder if air getting caught in the cassette may be one of my problems. Thanks in advance for your help...I sure need it. Kim From kim.tournear <@t> yahoo.com Mon Mar 28 20:25:34 2011 From: kim.tournear <@t> yahoo.com (Yahoo) Date: Mon Mar 28 20:25:50 2011 Subject: [Histonet] Lab week Message-ID: <9A81D404-4246-446F-BD3C-2BC81A64D9EC@yahoo.com> Can someone tell me when lab week is? Thanks in advance. Sent from the iPhone of Kim Tournear From sbreeden <@t> nmda.nmsu.edu Tue Mar 29 07:25:36 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 29 07:25:40 2011 Subject: [Histonet] Off-Topic: April Fool's Day Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47769@nmdamailsvr.nmda.ad.nmsu.edu> What's the best April Fool's Day prank (not causing permanent damage, please) you've ever played on your pathologist? You can consider this a "poll" but the underlying reason is that I desperately need to do something to mark this occasion - this being my last year in the lab. I have three pathologists that are in serious need of some foolishness. The only thing that comes to mind is MASH's episode with the charcoal on the oculars of Colonel Potter's binoculars. I need serious foolishness. Please feel free to respond off-line to avoid giving away your secrets and causing you to be discovered for your past transgressions. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From cmiller <@t> physlab.com Tue Mar 29 07:58:08 2011 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Mar 29 07:58:12 2011 Subject: [Histonet] RE: Off-Topic: April Fool's Day In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E47769@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E47769@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: We got a large specimen container, ones we use for mastectomies and colons (specimens the paths would need to gross in. We labeled it as a normal specimen, put a large piece of foam rubber inside and then put a rubber Rat inside. When the path opened it the foam rubber propelled the rat out of the specimen bucket LOL. That rat has since become our Histology mascot. We dress him up for various holidays. He is now sporting a green leprechaun hat and a beer mug necklace. I am looking for little bunny ears for Easter ever heard of the "the Cadbury rat"? :) Cheri Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, March 29, 2011 7:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Off-Topic: April Fool's Day What's the best April Fool's Day prank (not causing permanent damage, please) you've ever played on your pathologist? You can consider this a "poll" but the underlying reason is that I desperately need to do something to mark this occasion - this being my last year in the lab. I have three pathologists that are in serious need of some foolishness. The only thing that comes to mind is MASH's episode with the charcoal on the oculars of Colonel Potter's binoculars. I need serious foolishness. Please feel free to respond off-line to avoid giving away your secrets and causing you to be discovered for your past transgressions. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From settembr <@t> umdnj.edu Tue Mar 29 08:18:51 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Tue Mar 29 08:19:03 2011 Subject: [Histonet] Lab week In-Reply-To: <9A81D404-4246-446F-BD3C-2BC81A64D9EC@yahoo.com> References: <9A81D404-4246-446F-BD3C-2BC81A64D9EC@yahoo.com> Message-ID: Lab Week is April 24th through April 30th. Dana Settembre -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yahoo Sent: Monday, March 28, 2011 9:26 PM To: Histonet Subject: [Histonet] Lab week Can someone tell me when lab week is? Thanks in advance. Sent from the iPhone of Kim Tournear _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Tue Mar 29 08:32:27 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 29 08:32:31 2011 Subject: [Histonet] OT: April Fool's Nonsense Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> I like what I've gotten so far but I'm obviously a lot more evil than some of you... I'm thinking about how I could temporarily glue the radio station dial on the boss's radio to classical music in order to keep him from listening to Rush Limbaugh and Glen Beck. Or Saran Wrap over the urinal. Vaseline on office door handles. Filling one of their offices with bubble wrap. Gluing their desk drawer shut (the one with the food in it). Let's get SERIOUS here - I need more! I want people to be glad I've retired! Heh...heh... No pathologists were harmed in the making of this Tomfoolery. Darn it. And, yes, I do have work to do today... so far, anyway. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From jqb7 <@t> cdc.gov Tue Mar 29 09:06:47 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Mar 29 09:07:37 2011 Subject: [Histonet] RE: OT: April Fool's Nonsense In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: I don't know.....the constant playing of classical music would drive me nuts! Hopefully he doesn't have anyone within earshot. Or not: I guess he must if you know what he listens to, LOL! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, March 29, 2011 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: April Fool's Nonsense I like what I've gotten so far but I'm obviously a lot more evil than some of you... I'm thinking about how I could temporarily glue the radio station dial on the boss's radio to classical music in order to keep him from listening to Rush Limbaugh and Glen Beck. Or Saran Wrap over the urinal. Vaseline on office door handles. Filling one of their offices with bubble wrap. Gluing their desk drawer shut (the one with the food in it). Let's get SERIOUS here - I need more! I want people to be glad I've retired! Heh...heh... No pathologists were harmed in the making of this Tomfoolery. Darn it. And, yes, I do have work to do today... so far, anyway. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Tue Mar 29 09:17:28 2011 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Tue Mar 29 09:17:37 2011 Subject: [Histonet] OT: April Fool's Nonsense In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4D91E9F8.2030209@vneubert.com> http://www.youtube.com/watch?v=tPifEfo0GuI ? This might result in permanent damage to YOU as well, I guess... Remove the little wheels under the chairs? Mostly they are just plugged in and can easily be removed. Gelatine in the coffee maker? Whatever you do - make sure the list will know the results somehow ;) > I like what I've gotten so far but I'm obviously a lot more evil than > some of you... I'm thinking about how I could temporarily glue the radio > station dial on the boss's radio to classical music in order to keep him > from listening to Rush Limbaugh and Glen Beck. Or Saran Wrap over the > urinal. Vaseline on office door handles. Filling one of their offices > with bubble wrap. Gluing their desk drawer shut (the one with the food > in it). Let's get SERIOUS here - I need more! I want people to be glad > I've retired! Heh...heh... > > > > No pathologists were harmed in the making of this Tomfoolery. Darn it. > And, yes, I do have work to do today... so far, anyway. > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NLEMKE1 <@t> hfhs.org Tue Mar 29 09:32:13 2011 From: NLEMKE1 <@t> hfhs.org (Lemke, Nancy) Date: Tue Mar 29 09:32:34 2011 Subject: [Histonet] RE: OT: April Fool's Nonsense In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <278CE661-89E6-4DC4-9522-61B52C77D5C7@mimectl> The absolute best one I ever witnessed was when I worked in a histo lab that was next to the microbiology lab. To set up the director, some one came in with a stool specimen cup that was used in parasitology. It had a very official patient sticker on the side and was shown to the director. He was told that a test had been ordered that the tech did not recognize. The director was also unfamiliar with the requested test, so he asked what the exact nature of the specimen was, something that must have happened often, so the tech pulled the lid off of the container, whipped out a tongue depressor and stirred up the tan, gloppy contents, all the while looking like he was going to retch. He then scooped up a large dollop of the contents and ate it!!! The director came very close to apoplexy as did everyone else in the lab. At that point the tech shouted "April Fools!" and revealed that the container had butterscotch pudding in it! It took quite a while to calm down the lab, but the joke was gleefully told throughout the hospital! For strictly histo, it is always good to have a tray of slides of odd items, such as ffpe hotdogs, etc and see how far the diagnosis goes! Nancy W Lemke Research Coordinator Hermelin Brain Tumor Center Neurosurgery Research Henry Ford Hospital Detroit, MI (313) 916-8648 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara [sbreeden@nmda.nmsu.edu] Sent: Tuesday, March 29, 2011 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: April Fool's Nonsense I like what I've gotten so far but I'm obviously a lot more evil than some of you... I'm thinking about how I could temporarily glue the radio station dial on the boss's radio to classical music in order to keep him from listening to Rush Limbaugh and Glen Beck. Or Saran Wrap over the urinal. Vaseline on office door handles. Filling one of their offices with bubble wrap. Gluing their desk drawer shut (the one with the food in it). Let's get SERIOUS here - I need more! I want people to be glad I've retired! Heh...heh... No pathologists were harmed in the making of this Tomfoolery. Darn it. And, yes, I do have work to do today... so far, anyway. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From mab70 <@t> medschl.cam.ac.uk Tue Mar 29 10:08:41 2011 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Tue Mar 29 10:09:11 2011 Subject: [Histonet] RE: OT: April Fool's Nonsense In-Reply-To: <278CE661-89E6-4DC4-9522-61B52C77D5C7@mimectl> References: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> <278CE661-89E6-4DC4-9522-61B52C77D5C7@mimectl> Message-ID: I like that one! But I have to say I would have been feeling very, very squeamish had I been a fly on the wall. Sally here's to a happy retirement for you. If you come over to the UK, look me up in Cambridge. Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lemke, Nancy Sent: 29 March 2011 15:32 To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: OT: April Fool's Nonsense The absolute best one I ever witnessed was when I worked in a histo lab that was next to the microbiology lab. To set up the director, some one came in with a stool specimen cup that was used in parasitology. It had a very official patient sticker on the side and was shown to the director. He was told that a test had been ordered that the tech did not recognize. The director was also unfamiliar with the requested test, so he asked what the exact nature of the specimen was, something that must have happened often, so the tech pulled the lid off of the container, whipped out a tongue depressor and stirred up the tan, gloppy contents, all the while looking like he was going to retch. He then scooped up a large dollop of the contents and ate it!!! The director came very close to apoplexy as did everyone else in the lab. At that point the tech shouted "April Fools!" and revealed that the container had butterscotch pudding in it! It took quite a while to calm down the lab, but the joke was gleefully told throughout the hospital! For strictly histo, it is always good to have a tray of slides of odd items, such as ffpe hotdogs, etc and see how far the diagnosis goes! Nancy W Lemke Research Coordinator Hermelin Brain Tumor Center Neurosurgery Research Henry Ford Hospital Detroit, MI (313) 916-8648 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara [sbreeden@nmda.nmsu.edu] Sent: Tuesday, March 29, 2011 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: April Fool's Nonsense I like what I've gotten so far but I'm obviously a lot more evil than some of you... I'm thinking about how I could temporarily glue the radio station dial on the boss's radio to classical music in order to keep him from listening to Rush Limbaugh and Glen Beck. Or Saran Wrap over the urinal. Vaseline on office door handles. Filling one of their offices with bubble wrap. Gluing their desk drawer shut (the one with the food in it). Let's get SERIOUS here - I need more! I want people to be glad I've retired! Heh...heh... No pathologists were harmed in the making of this Tomfoolery. Darn it. And, yes, I do have work to do today... so far, anyway. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================================== ====== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Tue Mar 29 10:47:25 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Mar 29 10:47:43 2011 Subject: [Histonet] Florida Law Question In-Reply-To: References: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> <278CE661-89E6-4DC4-9522-61B52C77D5C7@mimectl> Message-ID: Hi Histonetters! Can anyone tell me (maybe even provide a req) as to whether or not a lab aide can run the Histos 5 microwave tissue processor? It's semi-automatic, which involves the person (lab aide, tech etc) moving a carousel of cassettes from one beaker to another. Any help is greatly appreciated! Michelle From JWeems <@t> sjha.org Tue Mar 29 10:53:39 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Mar 29 10:53:46 2011 Subject: [Histonet] RE: Off-Topic: April Fool's Day In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E47769@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E47769@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164081F174F21@CHEXCMS10.one.ads.che.org> We changed out family photos to see how long it would take for him to notice. But he saw right away and got us back.. Had the director tell us he had gone to HR. Was fun tho!! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, March 29, 2011 08:26 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Off-Topic: April Fool's Day What's the best April Fool's Day prank (not causing permanent damage, please) you've ever played on your pathologist? You can consider this a "poll" but the underlying reason is that I desperately need to do something to mark this occasion - this being my last year in the lab. I have three pathologists that are in serious need of some foolishness. The only thing that comes to mind is MASH's episode with the charcoal on the oculars of Colonel Potter's binoculars. I need serious foolishness. Please feel free to respond off-line to avoid giving away your secrets and causing you to be discovered for your past transgressions. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From sbreeden <@t> nmda.nmsu.edu Tue Mar 29 11:03:45 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 29 11:03:50 2011 Subject: [Histonet] End of April Fool's Day Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E47778@nmdamailsvr.nmda.ad.nmsu.edu> I've collected enough fodder to take me through the year 2015 - good thing I'll be retiring before that or I'll be "surplused"! Thanks to everyone who contributed and if you have anything further, send it to me directly so we don't clog up Histonet any more than I already have. I'll post a comprehensive list of suggestions that I've gotten... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From sbreeden <@t> nmda.nmsu.edu Tue Mar 29 13:13:07 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 29 13:13:12 2011 Subject: [Histonet] April 1st Fun Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E4777B@nmdamailsvr.nmda.ad.nmsu.edu> Dead cockroach on autopsy/necropsy table as legitimate specimen; goldfish processed as patient "Barry Cuda"; change family photos to total strangers; butterscotch pudding in (unused) specimen jar and subsequent tasting with applicator stick; activated charcoal on oculars (a la Colonel Potter in MASH); single hair attached to ocular with super-glue (tickling nose); cut fingertips off gloves; pumpkin/squash in animal shapes to slide; bug in block; foam-rubber-stuffed-box-with-toy-rat-to-burst-out-and-cause-startlement (toy rat to morph into histo lab mascot complete with appropriate holiday costume); process beef jerky as legitimate specimen; light coating of oil on 'scope oculars; - and the latest one I just received - a small slip of paper on the underside of the (computer's) mouse. Oh - the joy! What shall I do first?! Happy Annoying! This ought to cover the last several months I've not done the Friday Hour of Fuming... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From trathborne <@t> somerset-healthcare.com Tue Mar 29 14:05:47 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Mar 29 14:06:23 2011 Subject: [Histonet] April 1st Fun In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E4777B@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Thanks for sharing with us! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Breeden, Sara Sent: Tuesday, March 29, 2011 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] April 1st Fun Dead cockroach on autopsy/necropsy table as legitimate specimen; goldfish processed as patient "Barry Cuda"; change family photos to total strangers; butterscotch pudding in (unused) specimen jar and subsequent tasting with applicator stick; activated charcoal on oculars (a la Colonel Potter in MASH); single hair attached to ocular with super-glue (tickling nose); cut fingertips off gloves; pumpkin/squash in animal shapes to slide; bug in block; foam-rubber-stuffed-box-with-toy-rat-to-burst-out-and-cause-startlement (toy rat to morph into histo lab mascot complete with appropriate holiday costume); process beef jerky as legitimate specimen; light coating of oil on 'scope oculars; - and the latest one I just received - a small slip of paper on the underside of the (computer's) mouse. Oh - the joy! What shall I do first?! Happy Annoying! This ought to cover the last several months I've not done the Friday Hour of Fuming... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From Dawn.Gullifer <@t> osumc.edu Tue Mar 29 14:24:24 2011 From: Dawn.Gullifer <@t> osumc.edu (Gullifer, Dawn) Date: Tue Mar 29 14:24:31 2011 Subject: [Histonet] (no subject) Message-ID: <9D6A52275061274B8ED422D3D84745B702DCB418F8@EXMBOX-VP02.OSUMC.EDU> Can anyone tell me how and who they use to do radiation level checks in their laboratories? I was hoping to find something similar to the formalin monitoring but I am not having much luck. Dawn Gullifer BS, HT (ASCP) Laboratory Manager OSU Histology Lab, LLC 614-293-0358 office 614-293-0345 lab dawn.gullifer@osumc.edu This e-mail, including attachments, may contain information that is physician-patient privileged, proprietary or otherwise confidential. If you are not the intended recipient or his or her authorized agent, use and disclosure of this message are prohibited. Any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by replying to this e-mail and immediately delete the message and any attachments. From CIngles <@t> uwhealth.org Tue Mar 29 14:27:36 2011 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Mar 29 14:32:01 2011 Subject: [Histonet] RE: OT: April Fool's Nonsense References: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> <278CE661-89E6-4DC4-9522-61B52C77D5C7@mimectl> Message-ID: I get a bit creative with my joke slides and actually use old tissue to cut out shapes . Process, embedd, etc. and put them on slides and give to the doc. in the middle of his other cases. I have a pumpkin face on one with the slide labeled "O'Lantern, Jack" etc. I have also been known to use those cute bits of different shaped confetti and taped them to a slide for the docs. (coverslipping doesn't work too well as they are so thick). Claire From Bonnie.Whitaker <@t> osumc.edu Tue Mar 29 14:36:21 2011 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Tue Mar 29 14:36:27 2011 Subject: [Histonet] Histology Manager position Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670654D37666@EXMBOX-VP05.OSUMC.EDU> Hi Everyone, The position of Clinical Histology Laboratory Manager here at The Ohio State University Medical Center is still open: We are located in Columbus, OH which is a wonderful city with a population of 1.75 million. We are the 16th largest city in the US, without the traffic problems of some of the larger cities. There are 19 communities within 30 minutes of downtown Columbus. It really is a lovely area, complete with four seasons. The histology lab is a good lab, with a stable, hard-working staff. Our average block count is about 765/day and we have about 18 techs in histology. Also, we have great benefits!! Here is the official blurb: The OSUMC clinical histology laboratory is a functional laboratory within the Anatomic Pathology branch serving the pathology subspecialty divisions. The Manager will provide leadership for daily operations of the histology labs, manages human resources, financial and budget planning and preparation, new test development, quality improvement, safety programs, compliance program, successful inspection and accreditation processes, and ensuring current policies and procedures related to technical, operational, and administrative areas of the laboratory. For a complete position description and application instructions please go to http://medicalcenter.osu.edu/careers/ and search by posting number 354362. The Ohio State University is an equal opportunity, affirmative active employer. Women, minorities, veterans, and individuals with disabilities are encouraged to apply. Please don't hesitate to give me a call, or email me if you want more information. Thanks! Bonnie Bonnie Whitaker AP Operations Director The Ohio State University Medical Center Department of Pathology 614.293.5048 From laurie.colbert <@t> huntingtonhospital.com Tue Mar 29 14:44:50 2011 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Mar 29 14:44:55 2011 Subject: [Histonet] (no subject) In-Reply-To: <9D6A52275061274B8ED422D3D84745B702DCB418F8@EXMBOX-VP02.OSUMC.EDU> References: <9D6A52275061274B8ED422D3D84745B702DCB418F8@EXMBOX-VP02.OSUMC.EDU> Message-ID: <57BE698966D5C54EAE8612E8941D76830AD2AA84@EXCHANGE3.huntingtonhospital.com> We have a radiation officer in the hospital, and he came down and monitored for us. The levels were well below NRC guidelines, so he took the information to a Radiation Safety Committee meeting, and they are revising our procedure for us. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gullifer, Dawn Sent: Tuesday, March 29, 2011 12:24 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] (no subject) Can anyone tell me how and who they use to do radiation level checks in their laboratories? I was hoping to find something similar to the formalin monitoring but I am not having much luck. Dawn Gullifer BS, HT (ASCP) Laboratory Manager OSU Histology Lab, LLC 614-293-0358 office 614-293-0345 lab dawn.gullifer@osumc.edu This e-mail, including attachments, may contain information that is physician-patient privileged, proprietary or otherwise confidential. If you are not the intended recipient or his or her authorized agent, use and disclosure of this message are prohibited. Any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by replying to this e-mail and immediately delete the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Tue Mar 29 14:53:14 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Tue Mar 29 14:53:27 2011 Subject: [Histonet] April 1st Fun In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E4777B@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E4777B@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <80834B0C-09BB-4056-9795-26E57D50025B@imagesbyhopper.com> We had a patient we named Oscar Meyer... it was a piece of hot dog that we put in formalin, the doc grossed it, we cut it (and it cut very well, I might add!) and they got to the point of looking under the scope before they realized the joke! ;o) Michelle On Mar 29, 2011, at 2:13 PM, "Breeden, Sara" wrote: > Dead cockroach on autopsy/necropsy table as legitimate specimen; > goldfish processed as patient "Barry Cuda"; change family photos to > total strangers; butterscotch pudding in (unused) specimen jar and > subsequent tasting with applicator stick; activated charcoal on oculars > (a la Colonel Potter in MASH); single hair attached to ocular with > super-glue (tickling nose); cut fingertips off gloves; pumpkin/squash in > animal shapes to slide; bug in block; > foam-rubber-stuffed-box-with-toy-rat-to-burst-out-and-cause-startlement > (toy rat to morph into histo lab mascot complete with appropriate > holiday costume); process beef jerky as legitimate specimen; light > coating of oil on 'scope oculars; - and the latest one I just received - > a small slip of paper on the underside of the (computer's) mouse. Oh - > the joy! What shall I do first?! Happy Annoying! This ought to cover > the last several months I've not done the Friday Hour of Fuming... > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lhadley <@t> iupui.edu Tue Mar 29 14:53:53 2011 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Tue Mar 29 14:54:02 2011 Subject: [Histonet] RE: OT: April Fool's Nonsense In-Reply-To: References: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> <278CE661-89E6-4DC4-9522-61B52C77D5C7@mimectl> Message-ID: <8638FBDA16B0584D82AA21CD236FF97F20383415@IU-MSSG-MBX110.ads.iu.edu> We found a cockroach in our lab years ago. Placed it in a formalin container and put it in line to be gross. We filled out the patient history sheet with the name "Ima Roach". We thought the pathologist was going to break her neck getting out of the chair. LOL!!! Lee Ann Baldridge IUSM Indpls.,IN. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, March 29, 2011 3:28 PM To: Lemke, Nancy; Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: OT: April Fool's Nonsense I get a bit creative with my joke slides and actually use old tissue to cut out shapes . Process, embedd, etc. and put them on slides and give to the doc. in the middle of his other cases. I have a pumpkin face on one with the slide labeled "O'Lantern, Jack" etc. I have also been known to use those cute bits of different shaped confetti and taped them to a slide for the docs. (coverslipping doesn't work too well as they are so thick). Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Tue Mar 29 15:18:17 2011 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Tue Mar 29 15:18:23 2011 Subject: [Histonet] to microwave or not to microwave In-Reply-To: References: Message-ID: <688853.26306.qm@web43509.mail.sp1.yahoo.com> Hi Karla, We chose the Sakura XPress microwave processor. We have had it now for over two years. We eliminated an entire night shift (everyone works day shift now), we drastically reduced xylene usage (we only use it on the H&E stainer, we use none for processing anymore). The processing system takes about?1 hour and?30 minutes to process. I can run breast?tissue and GI biopsies on the same run. It is still truly amazing to me sometimes. ? We have cassettes coming off every 40 minutes to an hour now.?The techs rotate?so that we still have time to do special stains, IHC, frozens...etc. It has been a life saver for us. ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: Karla Sendelbach To: "histonet@lists.utsouthwestern.edu" Sent: Mon, March 28, 2011 12:44:02 PM Subject: [Histonet] to microwave or not to microwave We are evaluating rapid tissue processing instruments. I would appreciate any comments about microwave tissue process and rapid non microwave tissue process. Peloris II, STP 420ES and? Milestone Pathos come to mind. Also, is there anyone who switched from microwave processing to rapid non microwave tissue processing? Thank you, Dr. Sendelbach Appleton Medical Center Appleton Wisconsin 920.738.6294 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Tue Mar 29 15:20:02 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Tue Mar 29 15:20:05 2011 Subject: [Histonet] April 1st Fun In-Reply-To: <80834B0C-09BB-4056-9795-26E57D50025B@imagesbyhopper.com> References: <4D14F0FC9316DD41972D5F03C070908B02E4777B@nmdamailsvr.nmda.ad.nmsu.edu> <80834B0C-09BB-4056-9795-26E57D50025B@imagesbyhopper.com> Message-ID: The funny part is I hear that can be a fungus control!! =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Tuesday, March 29, 2011 2:53 PM To: Breeden, Sara Cc: Subject: Re: [Histonet] April 1st Fun We had a patient we named Oscar Meyer... it was a piece of hot dog that we put in formalin, the doc grossed it, we cut it (and it cut very well, I might add!) and they got to the point of looking under the scope before they realized the joke! ;o) Michelle On Mar 29, 2011, at 2:13 PM, "Breeden, Sara" wrote: > Dead cockroach on autopsy/necropsy table as legitimate specimen; > goldfish processed as patient "Barry Cuda"; change family photos to > total strangers; butterscotch pudding in (unused) specimen jar and > subsequent tasting with applicator stick; activated charcoal on oculars > (a la Colonel Potter in MASH); single hair attached to ocular with > super-glue (tickling nose); cut fingertips off gloves; pumpkin/squash in > animal shapes to slide; bug in block; > foam-rubber-stuffed-box-with-toy-rat-to-burst-out-and-cause-startlement > (toy rat to morph into histo lab mascot complete with appropriate > holiday costume); process beef jerky as legitimate specimen; light > coating of oil on 'scope oculars; - and the latest one I just received - > a small slip of paper on the underside of the (computer's) mouse. Oh - > the joy! What shall I do first?! Happy Annoying! This ought to cover > the last several months I've not done the Friday Hour of Fuming... > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ftryka <@t> tetonhospital.org Tue Mar 29 15:31:20 2011 From: ftryka <@t> tetonhospital.org (Tryka, A. Francine) Date: Tue Mar 29 15:35:45 2011 Subject: [Histonet] RE: OT: April Fool's Nonsense In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <90B1F64FAAAE7D4F8C28A8A523F50DAE05AA96111C99@SJHMAIL.tetonhosp.com> A joke that Hazel Horn and I, as well as others in the section, played on our pathologist chief years back... In sequence and separately, stated we smelled an odor in his office..... He looked around, especially at his rock collection on the window sill, and smelled each one, and could not detect an odor. After several hours of many of us playing this joke of smelling something in his office, we retrieved a stuffed rat (toy) from behind one of his cabinets. All in good fun, but I left before the next years April Fools Day rolled around. Franci Tryka ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara [sbreeden@nmda.nmsu.edu] Sent: Tuesday, March 29, 2011 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: April Fool's Nonsense I like what I've gotten so far but I'm obviously a lot more evil than some of you... I'm thinking about how I could temporarily glue the radio station dial on the boss's radio to classical music in order to keep him from listening to Rush Limbaugh and Glen Beck. Or Saran Wrap over the urinal. Vaseline on office door handles. Filling one of their offices with bubble wrap. Gluing their desk drawer shut (the one with the food in it). Let's get SERIOUS here - I need more! I want people to be glad I've retired! Heh...heh... No pathologists were harmed in the making of this Tomfoolery. Darn it. And, yes, I do have work to do today... so far, anyway. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Correspondence, including e-mail and other electronic communications, to and from employees and elected officials of the Teton County Hospital District, dba St. John's Medical Center, may be subject to disclosure under the Wyoming Public Records Act. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is strictly prohibited. If you are not the intended recipient, please notify the sender by reply e-mail and destroy all copies of the original message. Thank you for your cooperation. From gmartin <@t> marshallmedical.org Tue Mar 29 15:36:17 2011 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Tue Mar 29 15:36:30 2011 Subject: [Histonet] Peripheral smear Message-ID: <6ED9D4252F278841A0593D3D788AF24C0CF81CB7@mailsvr.MARSHMED.local> Our pathologist review peripheral smears for the clinical lab, and I'm wondering how other facilities handle the reporting. We presently receive the smear from a tech, the pathologist provides a comment and returns it to the lab. From jaylundgren <@t> gmail.com Tue Mar 29 16:04:12 2011 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Mar 29 16:04:17 2011 Subject: [Histonet] OT: April Fool's Nonsense In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E4776A@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Vaseline on the telephone earpiece is better than Vaseline on a doorknob. The best histo-oriented April fools joke is to fill a slide basket with blank slides. Go around your lab with another rack, like you are collecting slides to make a full rack to be stained. What a nice person! Be sure to get some prostate, kidney or other small, irreplaceable biopsies. Do the quick switch with the blank slide basket and "accidentally" drop the blank slide basket on the floor. Be sure to smash as many as possible. Then, "April Fool!". As a victim of this prank, I can guarantee that when a histotech hears a whole basket of slides hit the floor, they will not be thinking it is an April Fool's day joke. It is a sound that freezes the blood. You're welcome, Jay A. Lundgren, M.S., HTL (ASCP) From KMB01 <@t> grh.org Tue Mar 29 16:08:23 2011 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Tue Mar 29 16:08:27 2011 Subject: [Histonet] Thyroid slides Message-ID: Hi, I'm working on the thyroid for the HQIP for CAP and I've used several blocks but the cells looks like they are separating from each other. Any ideas? My Pathologist has not complained about them in the past. Thanks and Have a great day. Kathy Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. From cls71877 <@t> sbcglobal.net Tue Mar 29 16:10:03 2011 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Tue Mar 29 16:10:09 2011 Subject: [Histonet] Esophageal Brushings Message-ID: <181369.29788.qm@web81202.mail.mud.yahoo.com> Hello Histoland, I was wondering if anyone can help me with a CPT code question?? I run a small GI lab.? We have recently received esophageal brushings that were to rule out cancer, not Candida.? The pathologist indicated these should be stained with the Papinicalaou stain.? I was thinking per the archives, that this would be charged as an 88313 (non-organism stain) as we charge an 88312 for the fungal stain.? However, the other facilities around my area say that it would be charged as an 88104.? Can onyone offer me some guidance? Thank you all, Cristi From mpence <@t> grhs.net Tue Mar 29 16:18:39 2011 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Mar 29 16:18:43 2011 Subject: [Histonet] Esophageal Brushings In-Reply-To: <181369.29788.qm@web81202.mail.mud.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974B95@is-e2k3.grhs.net> I would charge an 88104 also. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson Sent: Tuesday, March 29, 2011 4:10 PM To: Histo Net Subject: [Histonet] Esophageal Brushings Hello Histoland, I was wondering if anyone can help me with a CPT code question?? I run a small GI lab.? We have recently received esophageal brushings that were to rule out cancer, not Candida.? The pathologist indicated these should be stained with the Papinicalaou stain.? I was thinking per the archives, that this would be charged as an 88313 (non-organism stain) as we charge an 88312 for the fungal stain.? However, the other facilities around my area say that it would be charged as an 88104.? Can onyone offer me some guidance? Thank you all, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Tue Mar 29 17:26:11 2011 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Tue Mar 29 17:26:15 2011 Subject: [Histonet] Anniston, AL Message-ID: <626578.6422.qm@web43506.mail.sp1.yahoo.com> Hello, ? I am trying to help a young?lady in the?Anniston area. She is interested in Histology and would like to try and find a job so that she can train and do one of the online courses.? ??Any contacts for her would be very helpful. Thanks! ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From shrivastavaanuradha <@t> hotmail.com Tue Mar 29 17:57:28 2011 From: shrivastavaanuradha <@t> hotmail.com (anuradha shrivastava) Date: Tue Mar 29 17:57:41 2011 Subject: [Histonet] PAS for fonfus Message-ID: Hi Histonetters, I am doing PAS for Fungus with SCHIFF?S and counter ?staining with Hematoxylin, the Hyphe look little paler than the spores/ yeast. Our Doc. wants the hyphe to be dark too. Any suggestion. Ikeep in Periodic acid for 5 min. nice rinse then in schiff?s for 15 min. then wash for 10 mi, in running tap water. then counterstain. Pleaseneed your expert advice. thank you. From shrivastavaanuradha <@t> hotmail.com Tue Mar 29 18:54:57 2011 From: shrivastavaanuradha <@t> hotmail.com (anuradha shrivastava) Date: Tue Mar 29 18:55:00 2011 Subject: [Histonet] PAS FOR FUNGUS Message-ID: Hi Every body, Please read the previous post as Fungus, there is a typo. Thanks. Anu Shrivastava From wdesalvo.cac <@t> hotmail.com Tue Mar 29 20:08:45 2011 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Tue Mar 29 20:08:49 2011 Subject: FW: [Histonet] to microwave or not to microwave In-Reply-To: References: Message-ID: This question comes up every so often and the good news is that there many good to great processors on the market and, like all things, they have their plus and minus issues. Before you decide which processor to purchase, you must first decide what you want the tissue processor to accomplish and identify the two or three most important issues that will most affect your process change. There is a growing trend in Histology to become more efficient/cost effective, reducing TAT and incorporating LEAN process improvement. I whole heartedly suggest you look to improve your process to match this trend and by doing so, you will be lead to rapid tissue processing in a most efficient LEAN way. Couple the previously mentioned trend with the ability to standardize processing for your routine formalin fixed samples and have the ability to process both formalin and molecular fixed samples on the same instrument; I suggest the Sakura Xpress (X50 or X120) rapid processors. These instruments provide continuous loading, small batch and require a small volume of reagent for processing and then discard. You must address grossing tissue samples to within specification, but that is an issue that you have to address with all tissue processors. A great advantage of incorporating this instrument into your LEAN process is the increased velocity of the workflow as the instruments are continuous load (no cleaning cycle between batches) and small batch (1 to 40 cassettes). Loading 1 or 2 cassettes when a STAT or RUSH cases arrives and completes fixation does not interrupt the process or require special handling. An important factor to consider is that continuous load processing does assist in workload leveling, which will lead to reducing employee stress, increase productivity and error reduction. All these factors contribute directly to reducing TAT. Add the often overlooked advantage of removing Xylene from your tissue processing, and again, I suggest you consider the Xpress. I was an early adopter (5+ yrs. use) and continue to use the X120 (2 units). I have not experienced any instrument performance or maintenance issues and we have never over processed tissues or incorrectly processed tissue samples. There is no other instrument that can facilitate processing in small batch or provide the continuous delivery of cassettes. You can do rapid processing with all of the instruments you are considering, but conventional, one reaction chamber instruments will limit the number of processing runs per day and that will lead to increased batch size. If you have interest or questions, feel free to contact me directly. I would be more than happy to share my experience with conventional, microwave and rapid tissue processing. William DeSalvo, B.S. HTL (ASCP) > From: Karla.Sendelbach@thedacare.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 28 Mar 2011 12:44:02 -0500 > Subject: [Histonet] to microwave or not to microwave > > We are evaluating rapid tissue processing instruments. I would appreciate any comments about microwave tissue process and rapid non microwave tissue process. Peloris II, STP 420ES and Milestone Pathos come to mind. Also, is there anyone who switched from microwave processing to rapid non microwave tissue processing? > Thank you, > Dr. Sendelbach > Appleton Medical Center > Appleton Wisconsin > 920.738.6294 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From diane.gladney <@t> us.army.mil Wed Mar 30 07:50:23 2011 From: diane.gladney <@t> us.army.mil (Gladney, Diane C Ms CIV USA MEDCOM MACH) Date: Wed Mar 30 07:50:36 2011 Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) Message-ID: Classification: UNCLASSIFIED Caveats: NONE Dear Histonetters, Our 11 year old cryostat is beginning to experience some unusual problems. Our Medical Maintenance says that they won't put a lot of money into fixing it because of the age of the unit. Therefore, I am asking for opinions, pros, cons of different brands of cryostats. I am interested in a cryostat that has the UV light or other source of easy decontamination. We don't do a lot of frozen sections but enough for us to have to have a good cryostat that is easy to operate and clean. Any comments or suggestions would be greatly appreciated. Thanks, Diane Gladney Diane C. Gladney, HT (ASCP) Histology Supervisor Department of Pathology Moncrief Army Community Hospital 4500 Stuart Street FT. Jackson, SC 29207 Phone: 803-751-2530 Fax: 803-751-7829 Classification: UNCLASSIFIED Caveats: NONE From dchihc <@t> yahoo.com Wed Mar 30 08:38:41 2011 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Wed Mar 30 08:38:45 2011 Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) In-Reply-To: References: Message-ID: <122646.831.qm@web43502.mail.sp1.yahoo.com> Hi Diane, ? We have been happy with the Leica cryostats over the years. We have an 1800 that is about 15 years old, an 1850 that is about 5 years old with the UV light for decontamination. ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Gladney, Diane C Ms CIV USA MEDCOM MACH" To: "histonet@lists.utsouthwestern.edu" Sent: Wed, March 30, 2011 7:50:23 AM Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) Classification:? UNCLASSIFIED Caveats: NONE Dear Histonetters, Our 11 year old cryostat is beginning to experience some unusual problems. Our Medical Maintenance says that they won't put a lot of money into fixing it because of the age of the unit. Therefore, I am asking for opinions, pros, cons of different brands of cryostats. I am interested in a cryostat that has the UV light or other source of easy decontamination. We don't do a lot of frozen sections but enough for us to have to have a good cryostat that is easy to operate and clean.? Any comments or suggestions would be greatly appreciated. Thanks, Diane Gladney Diane C. Gladney, HT (ASCP) Histology Supervisor Department of Pathology Moncrief Army Community Hospital 4500 Stuart Street FT. Jackson, SC? 29207 Phone:? 803-751-2530 Fax:? 803-751-7829 Classification:? UNCLASSIFIED Caveats: NONE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Wed Mar 30 08:47:34 2011 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Mar 30 08:47:42 2011 Subject: [Histonet] Esophageal Brushings In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974B95@is-e2k3.grhs.net> References: <181369.29788.qm@web81202.mail.mud.yahoo.com> <661949901A768E4F9CC16D8AF8F2838C03974B95@is-e2k3.grhs.net> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE02D93C7504@EXCHMBC2.ad.ah.local> I would make smears and a cell block(if enough specimen) and charge 88104 and 88305. Pap on the smear, H&E on the cell block. Jan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, March 29, 2011 4:19 PM To: Cristi stephenson; Histo Net Subject: RE: [Histonet] Esophageal Brushings I would charge an 88104 also. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson Sent: Tuesday, March 29, 2011 4:10 PM To: Histo Net Subject: [Histonet] Esophageal Brushings Hello Histoland, I was wondering if anyone can help me with a CPT code question? I run a small GI lab. We have recently received esophageal brushings that were to rule out cancer, not Candida. The pathologist indicated these should be stained with the Papinicalaou stain. I was thinking per the archives, that this would be charged as an 88313 (non-organism stain) as we charge an 88312 for the fungal stain. However, the other facilities around my area say that it would be charged as an 88104. Can onyone offer me some guidance? Thank you all, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Sharon.Davis-Devine <@t> carle.com Wed Mar 30 09:42:03 2011 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Wed Mar 30 09:42:09 2011 Subject: [Histonet] Ventana's Basal Cell Cocktail Message-ID: To all of you Histonetter's out there I need to know for those of you that are using the new Ventana antibody, CK34BE12 + p63, Basal Cell Cocktail, are you charging these as as one charge or two? Any information will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com From Wanda.Smith <@t> HCAhealthcare.com Wed Mar 30 09:45:29 2011 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Wed Mar 30 09:45:38 2011 Subject: [Histonet] RE: Peripheral smear In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C0CF81CB7@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C0CF81CB7@mailsvr.MARSHMED.local> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A275FF0C@NADCWPMSGCMS03.hca.corpad.net> We also transport smears from the clinical lab to our Pathologist and transport them back. The Hematology techs put the Pathologist's comments into our computer system for review by the clinicians. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Tuesday, March 29, 2011 4:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peripheral smear Our pathologist review peripheral smears for the clinical lab, and I'm wondering how other facilities handle the reporting. We presently receive the smear from a tech, the pathologist provides a comment and returns it to the lab. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laura.Miller <@t> leica-microsystems.com Wed Mar 30 10:00:49 2011 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Wed Mar 30 10:00:54 2011 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 03/25/2011 and will not return until 04/04/2011. I am on vacation until Monday, April 4th. Please contact David Archambault if you need immediate assistance. Thanks!!! ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From daystar0622 <@t> aol.com Wed Mar 30 10:56:07 2011 From: daystar0622 <@t> aol.com (daystar0622@aol.com) Date: Wed Mar 30 10:56:14 2011 Subject: [Histonet] Breast Processing Message-ID: <8CDBD0A1D7F79FE-DD4-531F@Webmail-m104.sysops.aol.com> Breast Processing: Looking to set up a breast protocol on our Sakura VIP processor. Looking for times/duration of specific solutions and types of solutions used (ie. PEN-FIX). From alyssa <@t> alliedsearchpartners.com Wed Mar 30 11:06:21 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Mar 30 11:06:28 2011 Subject: [Histonet] Whiteplains, NY: Histology Tech, IHC Techs, Leads & Manager Positions Message-ID: Allied Search Partners has been retained for the following searches. We have openings in *Histology/IHC, for techs, leads, and managers. * - Please email a copy of updated resume to alyssa@alliedsearchpartners.com for a full job description. - Please send availability for a phone screen with one of our recruiters. - Please indicate your salary expectations. - Referral bonuses are available for all positions so please pass this along to anyone you deem fit. We have the following positions available: *HISTOLOGY/IHC:* ** *IHC Manager* Whiteplains, NY area Monday-Friday, Day Shift, Full Time *NY license required *IHC Tech III (Lead)* Whiteplains, NY area Schedule, TBD *NY License Required *IHC Tech II * White Plains, NY area 7am-3:30pm Tuesday-Saturday 10am-6:30pm Monday-Friday *NY License Required *Histotech I* Whiteplains, NY area M-F 5am-1:30pm *NY license required **Ask us about our other histotech & managerial positions in WI, NC, CA, LA, FL. -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with ?Remove.? * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From LSebree <@t> uwhealth.org Wed Mar 30 11:09:33 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Mar 30 11:09:37 2011 Subject: [Histonet] Ventana's Basal Cell Cocktail In-Reply-To: References: Message-ID: <8C023B4AB999614BA4791BAEB26E273801002A19@UWHC-MAIL01.uwhis.hosp.wisc.edu> Sharon, We're actually doing a triple stain of HMWCK + p63 + p504S but charging as a single stain. I am advocating charging for at least 2 of the 3 stains (done in 2 separate runs) since one can easily differentiate between 2 different chromogen colors. The jury's still out. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, March 30, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana's Basal Cell Cocktail To all of you Histonetter's out there I need to know for those of you that are using the new Ventana antibody, CK34BE12 + p63, Basal Cell Cocktail, are you charging these as as one charge or two? Any information will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Mar 30 11:22:15 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Mar 30 11:22:19 2011 Subject: [Histonet] Re: Peripheral smear Message-ID: Gary Martin (where?) asks: >>Our pathologist[s] review peripheral smears for the clinical lab, and I'm wondering how other facilities handle the reporting. We presently receive the smear from a tech, the pathologist provides a comment and returns it to the lab.<< I take these VERY seriously. First, how NOT to do it: 1. Pathologist scribbles a tiny note in a space the size of a postage stamp, which then gets posted to the chart, or maybe doesn't. 2. Pathologist scribbles a note, which a technologist then types into the system (pathologist doesn't have access to the system). 3. Pathologist dictates a note, which is typed by a transcriptionist unfamiliar with the vocabulary, then put in the chart where the attending physician will never find it. Peripheral smear reviews are requests for consultation and should be treated as such. This consultation is billable (CPT 85060). There is a higher CPT code for more extensive consultation (80502) but I've never talked to anyone who'd heard of it. The pathologist should review the history to the extent necessary, and record this review in the note. (The patient doesn't live whose relevant history I can't summarize in one sprawling, badly written sentence.) The consult should include the basic data from the blood count that comes with the smear, since this information probably won't be simultaneously available to the physician reading the consult. The blood smear review should be focused in view of the information available. The pathologist should recommend such basic tests (reticulocyte count, vitamin B12, ferritin, direct antiglobulin test) as haven't been done. I prefer to type these consults myself, but most pathologists dictate them. A template would be worthwhile. The point here is - the pathologist is a consultant, not a technician, and should not regard themself as a technician nor allow themself to be treated as a technician. These reviews can be the best bargain in laboratory medicine, given that the pathologist would make better money flippin' burgers at McDonald's. I get enough feedback about them to know they're worthwhile. The opinions expressed are my own, and have not been vetted by anybody with an MBA. Bob Richmond Samurai Pathologist Knoxville TN From rsrichmond <@t> gmail.com Wed Mar 30 12:21:27 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Mar 30 12:21:32 2011 Subject: [Histonet] Re: Peripheral smear Message-ID: To add to my earlier diatribe: I do not want a slide dripping with immersion oil to mess up my microscope. I expect you to mount the smear in resin. Many histotechnologists are uncomfortable doing this, so I often coverslip the slide myself. I'll use immersion oil on the coverslipped slide if I need to - depending on the quality of my high-dry lens and on my elderly eyes. Bob Richmond Samurai Pathologist Knoxville TN From akemiat3377 <@t> yahoo.com Wed Mar 30 12:56:38 2011 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Mar 30 12:56:46 2011 Subject: [Histonet] Rita Humphrey Message-ID: <56464341-63ED-4938-BF42-3F808C10FEAB@yahoo.com> Hello Histoland! Does anyone know how to get in touch with Rita Humphrey, previously with Childrens Hospital, Birmingham, AL? I lost touch with her after she left Childrens and would like to re-connect. Thanks in advance, Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com From gmartin <@t> marshallmedical.org Wed Mar 30 13:18:16 2011 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Wed Mar 30 13:18:22 2011 Subject: [Histonet] RE: Histonet Digest, Vol 88, Issue 39 In-Reply-To: References: Message-ID: <6ED9D4252F278841A0593D3D788AF24C0CFDA913@mailsvr.MARSHMED.local> Thank to everyone who responded to my request for information concerning peripheral smear review. It seems that most respondents are doing this the way we presently do. I have to say that I agree totally with Dr. Richmond's method. I will be presenting the idea that these slides are "consults" not just a tehc issue. Many thanks Gary (in California) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 30, 2011 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 88, Issue 39 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Peripheral smear (Wanda.Smith@HCAhealthcare.com) 2. Laura Miller is Out of the Office. (Laura.Miller@leica-microsystems.com) 3. Breast Processing (daystar0622@aol.com) 4. Whiteplains, NY: Histology Tech, IHC Techs, Leads & Manager Positions (Alyssa Peterson) 5. RE: Ventana's Basal Cell Cocktail (Sebree Linda A) 6. Re: Peripheral smear (Robert Richmond) ---------------------------------------------------------------------- Message: 1 Date: Wed, 30 Mar 2011 09:45:29 -0500 From: Subject: [Histonet] RE: Peripheral smear To: , Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A275FF0C@NADCWPMSGCMS03.hca.corpad.ne t> Content-Type: text/plain; charset="us-ascii" We also transport smears from the clinical lab to our Pathologist and transport them back. The Hematology techs put the Pathologist's comments into our computer system for review by the clinicians. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Tuesday, March 29, 2011 4:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peripheral smear Our pathologist review peripheral smears for the clinical lab, and I'm wondering how other facilities handle the reporting. We presently receive the smear from a tech, the pathologist provides a comment and returns it to the lab. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 30 Mar 2011 10:00:49 -0500 From: Laura.Miller@leica-microsystems.com Subject: [Histonet] Laura Miller is Out of the Office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 03/25/2011 and will not return until 04/04/2011. I am on vacation until Monday, April 4th. Please contact David Archambault if you need immediate assistance. Thanks!!! ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 3 Date: Wed, 30 Mar 2011 11:56:07 -0400 (EDT) From: daystar0622@aol.com Subject: [Histonet] Breast Processing To: histonet@lists.utsouthwestern.edu Message-ID: <8CDBD0A1D7F79FE-DD4-531F@Webmail-m104.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Breast Processing: Looking to set up a breast protocol on our Sakura VIP processor. Looking for times/duration of specific solutions and types of solutions used (ie. PEN-FIX). ------------------------------ Message: 4 Date: Wed, 30 Mar 2011 12:06:21 -0400 From: Alyssa Peterson Subject: [Histonet] Whiteplains, NY: Histology Tech, IHC Techs, Leads & Manager Positions To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=windows-1252 Allied Search Partners has been retained for the following searches. We have openings in *Histology/IHC, for techs, leads, and managers. * - Please email a copy of updated resume to alyssa@alliedsearchpartners.com for a full job description. - Please send availability for a phone screen with one of our recruiters. - Please indicate your salary expectations. - Referral bonuses are available for all positions so please pass this along to anyone you deem fit. We have the following positions available: *HISTOLOGY/IHC:* ** *IHC Manager* Whiteplains, NY area Monday-Friday, Day Shift, Full Time *NY license required *IHC Tech III (Lead)* Whiteplains, NY area Schedule, TBD *NY License Required *IHC Tech II * White Plains, NY area 7am-3:30pm Tuesday-Saturday 10am-6:30pm Monday-Friday *NY License Required *Histotech I* Whiteplains, NY area M-F 5am-1:30pm *NY license required **Ask us about our other histotech & managerial positions in WI, NC, CA, LA, FL. -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with "Remove." * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ------------------------------ Message: 5 Date: Wed, 30 Mar 2011 11:09:33 -0500 From: "Sebree Linda A" Subject: RE: [Histonet] Ventana's Basal Cell Cocktail To: "Sharon.Davis-Devine" , Message-ID: <8C023B4AB999614BA4791BAEB26E273801002A19@UWHC-MAIL01.uwhis.hosp.wisc.ed u> Content-Type: text/plain; charset="us-ascii" Sharon, We're actually doing a triple stain of HMWCK + p63 + p504S but charging as a single stain. I am advocating charging for at least 2 of the 3 stains (done in 2 separate runs) since one can easily differentiate between 2 different chromogen colors. The jury's still out. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, March 30, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana's Basal Cell Cocktail To all of you Histonetter's out there I need to know for those of you that are using the new Ventana antibody, CK34BE12 + p63, Basal Cell Cocktail, are you charging these as as one charge or two? Any information will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 30 Mar 2011 12:22:15 -0400 From: Robert Richmond Subject: [Histonet] Re: Peripheral smear To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Gary Martin (where?) asks: >>Our pathologist[s] review peripheral smears for the clinical lab, and I'm wondering how other facilities handle the reporting. We presently receive the smear from a tech, the pathologist provides a comment and returns it to the lab.<< I take these VERY seriously. First, how NOT to do it: 1. Pathologist scribbles a tiny note in a space the size of a postage stamp, which then gets posted to the chart, or maybe doesn't. 2. Pathologist scribbles a note, which a technologist then types into the system (pathologist doesn't have access to the system). 3. Pathologist dictates a note, which is typed by a transcriptionist unfamiliar with the vocabulary, then put in the chart where the attending physician will never find it. Peripheral smear reviews are requests for consultation and should be treated as such. This consultation is billable (CPT 85060). There is a higher CPT code for more extensive consultation (80502) but I've never talked to anyone who'd heard of it. The pathologist should review the history to the extent necessary, and record this review in the note. (The patient doesn't live whose relevant history I can't summarize in one sprawling, badly written sentence.) The consult should include the basic data from the blood count that comes with the smear, since this information probably won't be simultaneously available to the physician reading the consult. The blood smear review should be focused in view of the information available. The pathologist should recommend such basic tests (reticulocyte count, vitamin B12, ferritin, direct antiglobulin test) as haven't been done. I prefer to type these consults myself, but most pathologists dictate them. A template would be worthwhile. The point here is - the pathologist is a consultant, not a technician, and should not regard themself as a technician nor allow themself to be treated as a technician. These reviews can be the best bargain in laboratory medicine, given that the pathologist would make better money flippin' burgers at McDonald's. I get enough feedback about them to know they're worthwhile. The opinions expressed are my own, and have not been vetted by anybody with an MBA. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 88, Issue 39 **************************************** From DKBoyd <@t> chs.net Wed Mar 30 13:32:46 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Mar 30 13:32:56 2011 Subject: [Histonet] Peripheral smear In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C0CF81CB7@mailsvr.MARSHMED.local> Message-ID: We accession the peripheral smear into our Clinical Microscopy module of the computer program. We stain the slide, coverslip it and give it to the pathologist with a hematology CBC print out. The pathologist interprets the slide and dictates the report. The transcriptionist types the report (much like any surgical report). The pathologist reviews the completed transcription and either makes corrections or electronically finalizes it. It then is available to all personnel to view in the computer system. We charge an 85060 for the professional fee. I am not aware of a technical fee we can charge. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Martin, Gary" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/29/2011 04:39 PM To cc Subject [Histonet] Peripheral smear Our pathologist review peripheral smears for the clinical lab, and I'm wondering how other facilities handle the reporting. We presently receive the smear from a tech, the pathologist provides a comment and returns it to the lab. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From histotech <@t> imagesbyhopper.com Wed Mar 30 13:39:48 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Mar 30 13:39:49 2011 Subject: [Histonet] Florida Law Question - again Message-ID: <00e901cbef09$d91be820$8b53b860$@imagesbyhopper.com> Hi Histonetters! I posted this the other day, but I guess it nested itself in the April Fools email. So, I thought I would post it again under its own heading! Can anyone tell me (maybe even provide a reg) as to whether or not a lab aide can run the Histos 5 microwave tissue processor? It's semi-automatic, which involves the person (lab aide, tech etc) moving a carousel of cassettes from one beaker to another. Does anyone know if this is considered to be waived, moderate or high complexity testing? Any help is greatly appreciated! Michelle From jsantiago <@t> bellsouth.net Wed Mar 30 14:38:40 2011 From: jsantiago <@t> bellsouth.net (Jerry Santiago) Date: Wed Mar 30 14:38:44 2011 Subject: [Histonet] Florida Law Question - again In-Reply-To: <00e901cbef09$d91be820$8b53b860$@imagesbyhopper.com> References: <00e901cbef09$d91be820$8b53b860$@imagesbyhopper.com> Message-ID: <265294.15790.qm@web180808.mail.gq1.yahoo.com> Hi Michelle, This issue has been discussed at the clinical board meeting and it has been said that?moving a carousel from one beaker to another is considered part of the specimen?processing steps, making it part of a licensed task. The?histology scope of practice includes as a licensed task specimen processing, see Rule 64B3-10.005 (11). This is the same issue with loading and unloading IHC instruments. The purpose of the specialty of histology is to process cellular and tissue components through methods of fixation, dehydration, embedding, microtomy, frozen sectioning, staining, and other related procedures and techniques employed in the preparation of smears, slides, and tissues. This specialty also encompasses methods for antigen detection and other molecular hybridization testing methods where the purpose is analysis and/or quantification of cellular and tissue components for interpretation by a qualified physician. Technicians licensed in histology are limited to the performance of specimen processing, embedding, cutting, routine and special histologic staining, frozen sectioning and mounting of preparations under the general supervision of a director, supervisor, or technologist. ? Hope this helps! ? Jerry Santiago, MSEd, HTL(ASCP)QIHC Florida State College Jacksonville Assistant Professor/HT Program Director 4501 Capper Road Jacksonville, FL 32218 Tel: 904-766-6528 Fax: 904-766-5573 e-mail:jsantiag@fscj.edu ________________________________ From: "histotech@imagesbyhopper.com" To: histonet@lists.utsouthwestern.edu Sent: Wed, March 30, 2011 2:39:48 PM Subject: [Histonet] Florida Law Question - again Hi Histonetters! I posted this the other day, but I guess it nested itself in the April Fools email.? So, I thought I would post it again under its own heading! Can anyone tell me (maybe even provide a reg) as to whether or not a lab aide can run the Histos 5 microwave tissue processor?? It's semi-automatic, which involves the person (lab aide, tech etc) moving a carousel of cassettes from one beaker to another. Does anyone know if this is considered to be waived, moderate or high complexity testing? Any help is greatly appreciated! Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ael <@t> unc.edu Wed Mar 30 14:40:19 2011 From: ael <@t> unc.edu (Ann Lowder) Date: Wed Mar 30 14:40:06 2011 Subject: [Histonet] to microwave or not to microwave Message-ID: <9A841190-EB9C-4ED5-8858-A0507FB135AC@unc.edu> Dr. Sendelbach, We had a microwave processor in the past, and switched back to a routine shortened biopsy run for several reasons. It is possible that the technology since then has improved to where you would not experience issues. However, microwave processing usually carries additional regulations (such as CAP for non-vented systems) and some machines require a lot of tech time to maintain. Most of the reagents suggested/sold for use with the microwave processors are alcohol or acetone based, which seemed to decrease the quality of our staining and sectioning. We have a shortened routine biopsy run on a vacuum processor that seems to do the job in the same amount of time with less additional maintenance and without the alcohol-fixed appearance in the tissue. Thank you, Ann Lowder, HT(ASCP) Message: 2 Date: Mon, 28 Mar 2011 12:44:02 -0500 From: Karla Sendelbach Subject: [Histonet] to microwave or not to microwave To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" We are evaluating rapid tissue processing instruments. I would appreciate any comments about microwave tissue process and rapid non microwave tissue process. Peloris II, STP 420ES and Milestone Pathos come to mind. Also, is there anyone who switched from microwave processing to rapid non microwave tissue processing? Thank you, Dr. Sendelbach Appleton Medical Center Appleton Wisconsin 920.738.6294 From Kimberly.Blundon <@t> drdc-rddc.gc.ca Wed Mar 30 14:55:15 2011 From: Kimberly.Blundon <@t> drdc-rddc.gc.ca (Blundon, Kimberly) Date: Wed Mar 30 14:55:20 2011 Subject: [Histonet] Re: Weigert's Iron Hematoxylin Message-ID: <42DFE1A029181B4B8CCBA7261B52D7656B0ED2@suffieldex01.suffield.drdc-rddc.gc.ca> Hi Histonetters, I have a quick question regarding Weigert's Iron Hematoxylin and Gill's 3 Hematoxylin. One of the guys here thinks that we can interchange these 2 stains. I am attempting to perform a Masson Trichrome stain and it calls for Weigert's Iron Hematoxylin. This person in my lab says to just use Gill's Hematoxylin in place of the Weigert's stain. Is this right? Thanks for your help Kimberly From Nacaela.Johnson <@t> USONCOLOGY.COM Wed Mar 30 15:03:50 2011 From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela) Date: Wed Mar 30 15:05:12 2011 Subject: [Histonet] Re: Weigert's Iron Hematoxylin In-Reply-To: <42DFE1A029181B4B8CCBA7261B52D7656B0ED2@suffieldex01.suffield.drdc-rddc.gc.ca> References: <42DFE1A029181B4B8CCBA7261B52D7656B0ED2@suffieldex01.suffield.drdc-rddc.gc.ca> Message-ID: <6DBD71C31D7E444482E5D3DFBC202D260324D680@txhous1eb012.uson.usoncology.int> Iron hematoxylin is more resistant to decolorization than aluminum hematoxylin. Since there are so many acid steps that follow the hematoxylin, it is wise to use the Weigert's so you do not have to worry about decolorizing the other tissue elements too far. However, if you optimize your stain to the use of the Gill's and the pathologists agree that staining intensity is optimal then it should be fine. I wouldn't do it without optimizing first. So overall, the two are not exactly interchangeable since some of the subsequent staining steps may have to be altered. Thanks, Nacaela Johnson, HTL (ASCP) Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: Nacaela.Johnson@USOncology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blundon, Kimberly Sent: Wednesday, March 30, 2011 2:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Weigert's Iron Hematoxylin Hi Histonetters, I have a quick question regarding Weigert's Iron Hematoxylin and Gill's 3 Hematoxylin. One of the guys here thinks that we can interchange these 2 stains. I am attempting to perform a Masson Trichrome stain and it calls for Weigert's Iron Hematoxylin. This person in my lab says to just use Gill's Hematoxylin in place of the Weigert's stain. Is this right? Thanks for your help Kimberly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From MHillmer <@t> dermwisconsin.com Wed Mar 30 15:08:57 2011 From: MHillmer <@t> dermwisconsin.com (Michael Hillmer) Date: Wed Mar 30 15:09:02 2011 Subject: [Histonet] New Position Message-ID: We have a brand new, 1st shift position open in our state of the art laboratory on the shores of Lake Michigan in Manitowoc. This position will be 1st shift, 4-9 hour days per week, with one floating day offer. We offer excellent wages and benefits. Please free to contact me if you or anyone that you may know is looking for a new position. We will also assist with relocation expenses. Michael Hillmer PHR HR Coordinator Dermatology Associates of Wisconsin Phone: (920)683-5278 Fax: (920)686-9674 Cell: (920)860-6360 The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. From GDawson <@t> dynacaremilwaukee.com Wed Mar 30 15:09:33 2011 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Mar 30 15:09:41 2011 Subject: [Histonet] Ventana's Basal Cell Cocktail In-Reply-To: <8C023B4AB999614BA4791BAEB26E273801002A19@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: <8C023B4AB999614BA4791BAEB26E273801002A19@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: Sharon, I do a CK34BE12 & P63 cocktail for basal cells & charge for only one stain. That being said, you can absolutely charge for 2 as P63 in nuclear & the CK34BE12 is cytoplasmic, so the pathologist can discern one from the other. I am considering setting it up as a double charge in the future if our volume for it continues to climb as it has been lately. Glen Dawson BS, HT(ASCP) & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, March 30, 2011 11:10 AM To: Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana's Basal Cell Cocktail Sharon, We're actually doing a triple stain of HMWCK + p63 + p504S but charging as a single stain. I am advocating charging for at least 2 of the 3 stains (done in 2 separate runs) since one can easily differentiate between 2 different chromogen colors. The jury's still out. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, March 30, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana's Basal Cell Cocktail To all of you Histonetter's out there I need to know for those of you that are using the new Ventana antibody, CK34BE12 + p63, Basal Cell Cocktail, are you charging these as as one charge or two? Any information will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fbozkurt <@t> gmail.com Wed Mar 30 15:12:00 2011 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Wed Mar 30 15:12:03 2011 Subject: [Histonet] Re: Weigert's Iron Hematoxylin In-Reply-To: <42DFE1A029181B4B8CCBA7261B52D7656B0ED2@suffieldex01.suffield.drdc-rddc.gc.ca> References: <42DFE1A029181B4B8CCBA7261B52D7656B0ED2@suffieldex01.suffield.drdc-rddc.gc.ca> Message-ID: Hi Kimberly, Imho because of the color, Weigert's Iron Hematoxylin is dark brown. Gill's 3 is blue. anilin blue is also blue. imho it would be better stain with Weigert's Iron Hematoxylin. On Wed, Mar 30, 2011 at 10:55 PM, Blundon, Kimberly < Kimberly.Blundon@drdc-rddc.gc.ca> wrote: > Hi Histonetters, > > > > I have a quick question regarding Weigert's Iron Hematoxylin and Gill's > 3 Hematoxylin. One of the guys here thinks that we can interchange these > 2 stains. I am attempting to perform a Masson Trichrome stain and it > calls for Weigert's Iron Hematoxylin. This person in my lab says to just > use Gill's Hematoxylin in place of the Weigert's stain. Is this right? > > > > Thanks for your help > > > > Kimberly > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mbplab <@t> yahoo.com Wed Mar 30 16:42:33 2011 From: mbplab <@t> yahoo.com (Mary Benoit) Date: Wed Mar 30 16:42:37 2011 Subject: [Histonet] in regards to review of peripheral smears Message-ID: <532270.31565.qm@web43137.mail.sp1.yahoo.com> In our lab, the hematology dept has a form that they present to the pathologist with a manual differential( if only an automated one was done?originally)?and CBC with abnormal results?, and a list of check boxes indicating whether they "agree with technologist interp" or if a "written consult is needed".? At?THAT time the case is then accessioned into the anatomic side, a report generated with pathologist's interp, recommendation etc and appropriate charges are made.? From eberledelphine <@t> hotmail.com Wed Mar 30 18:09:45 2011 From: eberledelphine <@t> hotmail.com (delphine eberle) Date: Wed Mar 30 18:09:54 2011 Subject: [Histonet] looking for a good anti-mouse CD3 for formalin fixed sections Message-ID: Hi, Could anyone suggest a good anti-mouse CD3? I am looking at staining aortic root frozen sections fixed with formalin. Thanks! Delphine Delphine Eberl? PhD UCSF Department of Vascular Surgery VA Medical Center - NCIRE Building 2 - room 410 4150 Clement Street San Francisco, CA 94121, USA From liz <@t> premierlab.com Wed Mar 30 18:22:13 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Mar 30 18:22:34 2011 Subject: [Histonet] looking for a good anti-mouse CD3 for formalin fixedsections In-Reply-To: Message-ID: The dako rabbit anti-CD3 cross reacts with mouse Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of delphine eberle Sent: Wednesday, March 30, 2011 5:10 PM To: histonet Subject: [Histonet] looking for a good anti-mouse CD3 for formalin fixedsections Hi, Could anyone suggest a good anti-mouse CD3? I am looking at staining aortic root frozen sections fixed with formalin. Thanks! Delphine Delphine Eberl? PhD UCSF Department of Vascular Surgery VA Medical Center - NCIRE Building 2 - room 410 4150 Clement Street San Francisco, CA 94121, USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sadey <@t> hotmail.ca Wed Mar 30 19:04:50 2011 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Wed Mar 30 19:04:53 2011 Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) In-Reply-To: <122646.831.qm@web43502.mail.sp1.yahoo.com> References: , <122646.831.qm@web43502.mail.sp1.yahoo.com> Message-ID: Hi Diane, We just purchased a cryostat from Sakura, we love it. Great features. > Date: Wed, 30 Mar 2011 06:38:41 -0700 > From: dchihc@yahoo.com > To: diane.gladney@us.army.mil; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) > CC: > > Hi Diane, > We have been happy with the Leica cryostats over the years. We have an 1800 > that is about 15 years old, an 1850 that is about 5 years old with the UV light > for decontamination. > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > ________________________________ > From: "Gladney, Diane C Ms CIV USA MEDCOM MACH" > To: "histonet@lists.utsouthwestern.edu" > Sent: Wed, March 30, 2011 7:50:23 AM > Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) > > Classification: UNCLASSIFIED > Caveats: NONE > > Dear Histonetters, > > Our 11 year old cryostat is beginning to experience some unusual problems. Our > Medical Maintenance says that they won't put a lot of money into fixing it > because of the age of the unit. Therefore, I am asking for opinions, pros, cons > of different brands of cryostats. I am interested in a cryostat that has the UV > light or other source of easy decontamination. We don't do a lot of frozen > sections but enough for us to have to have a good cryostat that is easy to > operate and clean. Any comments or suggestions would be greatly appreciated. > > Thanks, > Diane Gladney > > > Diane C. Gladney, HT (ASCP) > Histology Supervisor > Department of Pathology > Moncrief Army Community Hospital > 4500 Stuart Street > FT. Jackson, SC 29207 > > Phone: 803-751-2530 > Fax: 803-751-7829 > > > > > Classification: UNCLASSIFIED > Caveats: NONE > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Wed Mar 30 20:11:40 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Wed Mar 30 20:11:44 2011 Subject: [Histonet] Re: Cryostat Feedback Needed Message-ID: <703102.73760.qm@web120619.mail.ne1.yahoo.com> Hi Diane - I have been using the Leica 1800, 1850 and 1510 since 1992. I cut between 160 to 210 FS blocks a week and have had very few problems with any of them. In my new lab I have a 1510 and an 1850 UV. It's hard to beat a Leica. I'm just saying. . . Yours, Dave From salim.inan <@t> gmail.com Thu Mar 31 05:08:19 2011 From: salim.inan <@t> gmail.com (Salim Yalcin Inan) Date: Thu Mar 31 05:08:23 2011 Subject: [Histonet] Ordering Chemicals Message-ID: Dear Histonet Members, I am trying to build a neuroscience laboratory from scratch in Turkey. As you can imagine, there are many different things, I have to deal with. I have some questions about ordering basic chemicals for histology. Since there are many different qualities of the same product (for example; 21 different NaOH in Sigma catalog), I am not sure which one is better for histology and immunohistochemistry. Could it be possible to write me the company name and the catalog number of the chemicals, that I have listed below, so I can order correct ones? Any other suggestions and advices, as well as donations would greatly be appreciated. Thank you very much in advance. Sincerely, Salim Yalcin Inan, PhD Department of Pharmacology Meram Faculty of Medicine University of Selcuk 42080 Meram, Konya, Turkey Phone: +90 507 206 9662 (cellular) Phone: +90 332 223 6624 (work) E-mail: salim.inan@gmail.com FOR PBS SOLUTION - Sodium Chloride - Potassium Chloride - Sodium Phosphate (monobasic) - Potassium Phosphate (monobasic) FOR SUCROSE SOLUTION - Sucrose - Sodium Azide FOR PFA SOLUTION - Paraformaldehyte - Sodium Hydroxide - Hydrogen Chloride FOR CRESYL VIOLET STAINING - Cresyl Violet - Glacial Acedic Acid - Absolute Ethanol - Xylene or Histoclear - Mounting media (Permount) - Ready to use microscope slides and cover slips From lbustamante <@t> cvm.tamu.edu Thu Mar 31 09:29:53 2011 From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante) Date: Thu Mar 31 09:30:00 2011 Subject: [Histonet] National Average Salary Message-ID: <4D944991020000B9000FB00F@CVM.TAMU.EDU> Can anyone please send me the National Average Salary chart for Histotechnician and HT Supervisor with more than 5 years experience? Thank you very much. Lin Bustamante Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 From schlea <@t> sage.edu Thu Mar 31 09:31:08 2011 From: schlea <@t> sage.edu (Anna Schleifstein) Date: Thu Mar 31 09:31:13 2011 Subject: [Histonet] Incubator sale Message-ID: <6622785.9321301581868589.JavaMail.root@ccprodapp31> In an attempt to make room for new equipment, Sage colleges is selling a 4.5 cu ft Fisher Isotemp Forced-Air Incubator model 650F. Anyone interested please contact me by email or at the number below. Anna Schleifstein SCA Bio Lab Coordinator Sage Colleges Albany NY 12208 518- 292-1748 schlea@sage.edu From ruebenjcarter <@t> gmail.com Thu Mar 31 09:31:27 2011 From: ruebenjcarter <@t> gmail.com (R C) Date: Thu Mar 31 09:31:31 2011 Subject: [Histonet] HT and PA Positions in Chicago area? Message-ID: Hi. Does anyone know of Histologist and Pathologist Assistant positions in the Chicago area? Thanks. From POWELL_SA <@t> mercer.edu Thu Mar 31 09:35:20 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Thu Mar 31 09:35:26 2011 Subject: [Histonet] National Average Salary In-Reply-To: <4D944991020000B9000FB00F@CVM.TAMU.EDU> References: <4D944991020000B9000FB00F@CVM.TAMU.EDU> Message-ID: <9BF995BC0E47744E9673A41486E24EE238D4F5BB3D@MERCERMAIL.MercerU.local> Salary survey is in the March 2011 issue of LabMedicine from ASCP. You can also get it from www.ascp.org web site. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante Sent: Thursday, March 31, 2011 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] National Average Salary Can anyone please send me the National Average Salary chart for Histotechnician and HT Supervisor with more than 5 years experience? Thank you very much. Lin Bustamante Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Thu Mar 31 09:47:40 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Thu Mar 31 09:47:44 2011 Subject: [Histonet] National Average Salary In-Reply-To: <9BF995BC0E47744E9673A41486E24EE238D4F5BB3D@MERCERMAIL.MercerU.local> References: <4D944991020000B9000FB00F@CVM.TAMU.EDU> <9BF995BC0E47744E9673A41486E24EE238D4F5BB3D@MERCERMAIL.MercerU.local> Message-ID: So apparently I'm underpaid =) Good Luck convincing employers of this! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Thursday, March 31, 2011 9:35 AM To: Lin Bustamante Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] National Average Salary Salary survey is in the March 2011 issue of LabMedicine from ASCP. You can also get it from www.ascp.org web site. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante Sent: Thursday, March 31, 2011 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] National Average Salary Can anyone please send me the National Average Salary chart for Histotechnician and HT Supervisor with more than 5 years experience? Thank you very much. Lin Bustamante Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Thu Mar 31 09:53:27 2011 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Thu Mar 31 09:53:31 2011 Subject: [Histonet] National Average Salary In-Reply-To: References: <4D944991020000B9000FB00F@CVM.TAMU.EDU> <9BF995BC0E47744E9673A41486E24EE238D4F5BB3D@MERCERMAIL.MercerU.local> Message-ID: <9BF995BC0E47744E9673A41486E24EE238D4F5BB94@MERCERMAIL.MercerU.local> Oh I think you will find that 98% of us are underpaid. :) -----Original Message----- From: sgoebel@mirnarx.com [mailto:sgoebel@mirnarx.com] Sent: Thursday, March 31, 2011 10:48 AM To: Shirley A. Powell; lbustamante@cvm.tamu.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] National Average Salary So apparently I'm underpaid =) Good Luck convincing employers of this! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Thursday, March 31, 2011 9:35 AM To: Lin Bustamante Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] National Average Salary Salary survey is in the March 2011 issue of LabMedicine from ASCP. You can also get it from www.ascp.org web site. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante Sent: Thursday, March 31, 2011 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] National Average Salary Can anyone please send me the National Average Salary chart for Histotechnician and HT Supervisor with more than 5 years experience? Thank you very much. Lin Bustamante Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Thu Mar 31 10:25:01 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Mar 31 10:25:05 2011 Subject: [Histonet] Peripheral smear review Message-ID: Gary Martin, I'd be very interested in knowing what response you get to your presentation. I'm certainly available by e-mail if anybody wants to know more. Remember when you copy my note to include the comment about coverslipping which I posted later. I'd add further: The pathologist's task in reviewing the slides is to correlate the patient's history, the microscopic appearance of the blood smear, and the blood count data, in order to produce a consultation report useful to the attending physician in caring for the patient. Bob Richmond Samurai Pathologist Knoxville TN rsrichmond@gmail.com ************************************************** From: "Martin, Gary" Subject: [Histonet] RE: Histonet Digest, Vol 88, Issue 39 To: Thanks to everyone who responded to my request for information concerning peripheral smear review. It seems that most respondents are doing this the way we presently do. I have to say that I agree totally with Dr. Richmond's method. I will be presenting the idea that these slides are "consults" not just a tech issue. Many thanks Gary (in California) From lbustamante <@t> cvm.tamu.edu Thu Mar 31 10:29:38 2011 From: lbustamante <@t> cvm.tamu.edu (Lin Bustamante) Date: Thu Mar 31 10:29:57 2011 Subject: [Histonet] National Average Salary Message-ID: <4D945795020000B9000FB08A@CVM.TAMU.EDU> Thank you very much for the information. Lin Bustamante. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 >>> "Shirley A. Powell" 03/31/11 9:35 AM >>> Salary survey is in the March 2011 issue of LabMedicine from ASCP. You can also get it from www.ascp.org web site. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante Sent: Thursday, March 31, 2011 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] National Average Salary Can anyone please send me the National Average Salary chart for Histotechnician and HT Supervisor with more than 5 years experience? Thank you very much. Lin Bustamante Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Thu Mar 31 12:02:09 2011 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Thu Mar 31 12:02:38 2011 Subject: [Histonet] I Message-ID: I will be out of the office starting 03/31/2011 and will not return until 04/01/2011. In my absence please ask for Mary Campbell . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. From sujatas <@t> scripps.edu Thu Mar 31 12:39:36 2011 From: sujatas <@t> scripps.edu (Sujata Sovani) Date: Thu Mar 31 12:41:25 2011 Subject: [Histonet] Acriflavine staining of alginate for mucopolysaccharides Message-ID: Hi All, I am new to histology. I came across the protocol below for staining GAGs (I plan to use that for alginate) in an online book 'Theory and practice of histological techniques', By John D. Bancroft, Marilyn Gamble. The protocol is by Hollander dated 1963. Would like to know if anyone has used this protocol before? Any tips/specific details might be helpful. The 20% HCl - does it mean 20% of 37.3% concentrated hydrochloric acid? Any details about acriflavine to be used would be helpful. Thank you. Regards, Sujata ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ The protocol is as below: Protocol Acriflavine-DMAB method for sulfatide (Hollander 1963) Fixation and sections Post-fixed cryostat sections; formal calcium-fixed frozen sections Prepa?ration of reagents 1.Acriflavine stock solution Acriflavine 100mg Distilled water at 80 deg C 20ml Store in dark at 4 deg C 2.Acriflavine working solution 0.1M citrate-HCl buffer pH 2.5 99ml Stock acriflavine solution 1ml 3.DMAB solution p-dimethylaminobenzaldehyde 0.6g 20% HCl 30ml Isopropanol 70ml Method 1. Mount sections onto slides 2. Stain for 6 minutes in acriflavine solution 3. Differentiate for 1 minute in two changes of 70% isopropanol 4. Treat with DMAB reagent for 30-45 seconds 5. Rinse in distilled water for 2-3 minutes 6. Counterstain nuclei in Mayer?s or Carazzi?s hematoxylin 7. Blue in tap water, rinse in distilled water and mount sections in glycerin jelly. From jameshart77 <@t> gmail.com Thu Mar 31 12:42:34 2011 From: jameshart77 <@t> gmail.com (James Hart) Date: Thu Mar 31 12:42:38 2011 Subject: [Histonet] GFP labelled cells after decalcification...are they still labelled? Message-ID: Hi All A first time user of Histonet here -with a quick question for research histologists... Has anyone ever attempted to locate GFP expressing cells in bone-marrow sections after decalcification and paraffin embedding? (I guess my question really is: Does the decalcification process destroy or weaken the GFP signal) Any advice would be greatly appreciated! Regards James Dr James Hart BVSc MS Comparative Orthopaedics, Cornell University From talulahgosh <@t> gmail.com Thu Mar 31 12:46:38 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Mar 31 12:46:42 2011 Subject: [Histonet] cryostat repair Message-ID: Our cryostat (CM3050S) is making a terrible noise when the fan stops running, yet I don't want to call Leica (the manufacturer) because they charge more money than anyone could ever afford. How would I go about finding someone who could repair a cryostat in Pittsburgh? I don't think it's the refrigeration that's broken, it sounds like something mechanical. Any suggestions? Has anyone ever tried to open the back of one to see if it's fixable? I'm no mechanic, but I think I can spot a worn belt or loose screw. I'm just not sure I want to open it up myself. Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins From anonwums1 <@t> gmail.com Thu Mar 31 12:49:27 2011 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Mar 31 12:49:31 2011 Subject: [Histonet] GFP labelled cells after decalcification...are they still labelled? In-Reply-To: References: Message-ID: Hi James, I do this all the time in mouse bones. I've found that EGFP is very robust, and it can survive fixation with PFA and Zn buffered formalin, as well as decalcification with EDTA or formic acid, followed by paraffin embedding. However, the endogenous fluorescence is highly quenched by this process and barely detectable over background if at all. In order to detect it post embedding, I use the chicken anti-GFP polyclonal from Abcam, and a fluorophore conjugated donkey anti-chicken IgY with very good results. If you want endogenous fluorescence, you can fix the bones in 4% PFA and decalcify using EDTA, and use frozen sections. I hope this helps, Adam On Thu, Mar 31, 2011 at 12:42 PM, James Hart wrote: > Hi All > > A first time user of Histonet here -with a quick question for research > histologists... > > Has anyone ever attempted to locate GFP expressing cells in > bone-marrow sections after decalcification and paraffin embedding? (I guess > my question really is: Does the decalcification process destroy or weaken > the GFP signal) > > Any advice would be greatly appreciated! > > Regards > > James > > Dr James Hart BVSc MS > Comparative Orthopaedics, Cornell University > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cgill <@t> marylandgeneral.org Thu Mar 31 12:53:16 2011 From: cgill <@t> marylandgeneral.org (Gill, Caula A.) Date: Thu Mar 31 12:53:19 2011 Subject: [Histonet] Paula Wilder Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9F5A@MDGEN-EXCH1.marylandgeneral.org> Paula Wilder if you get this message please call Dianna Fetter at 443-552-2968. I know you have retired but I am trying to reach you. I would like to speak with you. Happy retirement Dianna P.S I'm writing this from Caula's computer...Hence where the message is from From mcauliff <@t> umdnj.edu Thu Mar 31 13:09:17 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Mar 31 13:05:58 2011 Subject: [Histonet] Acriflavine staining of alginate for mucopolysaccharides In-Reply-To: References: Message-ID: <4D94C34D.4020807@umdnj.edu> Greetings Sujata: On 3/31/2011 1:39 PM, Sujata Sovani wrote: > Hi All, > > I am new to histology. > I came across the protocol below for staining GAGs (I plan to use that for alginate) in an online book 'Theory and practice of histological techniques', By John D. Bancroft, Marilyn Gamble. A good book. > The protocol is by Hollander dated 1963. A very old, but not necessarily bad, method. Why do you want to use this particular method? There are simpler ways to do this. Alcohol fixation might retain GAGs better ... how about staining with 1% Alcian Blue in 3% acetic acid? > Would like to know if anyone has used this protocol before? Any tips/specific details might be helpful. > The 20% HCl - does it mean 20% of 37.3% concentrated hydrochloric acid? Yes. > Any details about acriflavine to be used would be helpful. Can't help you there. I'll bet DMAB is very toxic. Geoff > Thank you. > Regards, > Sujata > > ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ > > The protocol is as below: > > Protocol > > Acriflavine-DMAB method for sulfatide (Hollander 1963) > > Fixation and sections > Post-fixed cryostat sections; formal calcium-fixed frozen sections > > Prepa?ration of reagents > > 1.Acriflavine stock solution > Acriflavine 100mg > Distilled water at 80 deg C 20ml > > Store in dark at 4 deg C > > 2.Acriflavine working solution > 0.1M citrate-HCl buffer pH 2.5 99ml > Stock acriflavine solution 1ml > > 3.DMAB solution > p-dimethylaminobenzaldehyde 0.6g > 20% HCl 30ml > Isopropanol 70ml > > Method > > > 1. Mount sections onto slides > 2. Stain for 6 minutes in acriflavine solution > 3. Differentiate for 1 minute in two changes of 70% isopropanol > 4. Treat with DMAB reagent for 30-45 seconds > 5. Rinse in distilled water for 2-3 minutes > 6. Counterstain nuclei in Mayer?s or Carazzi?s hematoxylin > 7. Blue in tap water, rinse in distilled water and mount sections in glycerin jelly. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From dhewitt <@t> hvhs.org Thu Mar 31 13:15:12 2011 From: dhewitt <@t> hvhs.org (DANIEL HEWITT) Date: Thu Mar 31 13:15:21 2011 Subject: [Histonet] cryostat repair In-Reply-To: References: Message-ID: <7DDB5AB36CBC574D8D680806E7BBE58B9B733B@MX-HVB-02.hvhs.org> Emily You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars and do most of our repairs. Ray does an excellent job at a good price. It sounds like the bearings in your fan or maybe the compressor. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, March 31, 2011 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat repair Our cryostat (CM3050S) is making a terrible noise when the fan stops running, yet I don't want to call Leica (the manufacturer) because they charge more money than anyone could ever afford. How would I go about finding someone who could repair a cryostat in Pittsburgh? I don't think it's the refrigeration that's broken, it sounds like something mechanical. Any suggestions? Has anyone ever tried to open the back of one to see if it's fixable? I'm no mechanic, but I think I can spot a worn belt or loose screw. I'm just not sure I want to open it up myself. Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ksbrown <@t> lmhosp.org Thu Mar 31 13:23:39 2011 From: ksbrown <@t> lmhosp.org (Brown, Kimberly) Date: Thu Mar 31 13:23:29 2011 Subject: [Histonet] CoPath Users in CT (Eastern) Message-ID: Hi, I am looking for a hospital/lab which uses the CoPath system for daily operations within the Eastern parts of CT. Kimberly Brown Histology Lab Manager Lawrence and Memorial Hospital Phone # 860-442-0711 ext. 4383 This message (and any included attachments) is from Lawrence & Memorial Corporation, Inc. or one of its affiliates and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. From bouchalr <@t> wvuhealthcare.com Thu Mar 31 13:38:05 2011 From: bouchalr <@t> wvuhealthcare.com (Bouchal, Rena L) Date: Thu Mar 31 13:37:23 2011 Subject: [Histonet] cryostat repair In-Reply-To: <7DDB5AB36CBC574D8D680806E7BBE58B9B733B@MX-HVB-02.hvhs.org> References: <7DDB5AB36CBC574D8D680806E7BBE58B9B733B@MX-HVB-02.hvhs.org> Message-ID: We are using Tech One Biomedical... service reps in Pgh....for almost all of our routine instrument service.... really good and half the price of the vendors! Their number is 866-497-3033 or www.techoneweb.com Please note that my email address as of Jan 3, 2011 is bouchalr@wvuhealthcare.com . Please make the appropriate changes in your address book. Rena Bouchal, M.S. Anatomic Pathology Manager West Virginia University Hospitals 304-293-7765 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DANIEL HEWITT Sent: Thursday, March 31, 2011 2:15 PM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat repair Emily You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars and do most of our repairs. Ray does an excellent job at a good price. It sounds like the bearings in your fan or maybe the compressor. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, March 31, 2011 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat repair Our cryostat (CM3050S) is making a terrible noise when the fan stops running, yet I don't want to call Leica (the manufacturer) because they charge more money than anyone could ever afford. How would I go about finding someone who could repair a cryostat in Pittsburgh? I don't think it's the refrigeration that's broken, it sounds like something mechanical. Any suggestions? Has anyone ever tried to open the back of one to see if it's fixable? I'm no mechanic, but I think I can spot a worn belt or loose screw. I'm just not sure I want to open it up myself. Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From b-frederick <@t> northwestern.edu Thu Mar 31 14:42:56 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Mar 31 14:43:00 2011 Subject: [Histonet] cryostat repair In-Reply-To: References: <7DDB5AB36CBC574D8D680806E7BBE58B9B733B@MX-HVB-02.hvhs.org> Message-ID: <003e01cbefdb$d56d2f90$80478eb0$@northwestern.edu> We use them too- Illinois office. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bouchal, Rena L Sent: Thursday, March 31, 2011 1:38 PM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat repair We are using Tech One Biomedical... service reps in Pgh....for almost all of our routine instrument service.... really good and half the price of the vendors! Their number is 866-497-3033 or www.techoneweb.com Please note that my email address as of Jan 3, 2011 is bouchalr@wvuhealthcare.com . Please make the appropriate changes in your address book. Rena Bouchal, M.S. Anatomic Pathology Manager West Virginia University Hospitals 304-293-7765 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DANIEL HEWITT Sent: Thursday, March 31, 2011 2:15 PM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat repair Emily You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars and do most of our repairs. Ray does an excellent job at a good price. It sounds like the bearings in your fan or maybe the compressor. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, March 31, 2011 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat repair Our cryostat (CM3050S) is making a terrible noise when the fan stops running, yet I don't want to call Leica (the manufacturer) because they charge more money than anyone could ever afford. How would I go about finding someone who could repair a cryostat in Pittsburgh? I don't think it's the refrigeration that's broken, it sounds like something mechanical. Any suggestions? Has anyone ever tried to open the back of one to see if it's fixable? I'm no mechanic, but I think I can spot a worn belt or loose screw. I'm just not sure I want to open it up myself. Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Thu Mar 31 14:45:05 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Mar 31 14:45:08 2011 Subject: [Histonet] another money saving question Message-ID: Is there any way to extend the life of PAP pens? Add a little xylene to the tip or soak it in something? I feel like they run out so fast. Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins From srwilkes <@t> gmail.com Thu Mar 31 15:09:39 2011 From: srwilkes <@t> gmail.com (Steven Wilkes) Date: Thu Mar 31 15:09:43 2011 Subject: [Histonet] Kappa/Lambda ISH on Ventana Message-ID: Hi Histonet! I have been tasked with validating Kappa and Lambda on our Ventana Benchmark XT machines. Does anyone who uses this platform have suggestions as to which Ventana protease works best (1, 2 or 3)? Additionally, any other information concerning the optimization (suggested incubation times, RNA regions etc.) would be greatly appreciated. Thanks in advance. Steven srwilkes@gmail.com From Pitts.Jaclyn <@t> mayo.edu Thu Mar 31 15:22:55 2011 From: Pitts.Jaclyn <@t> mayo.edu (Pitts, Jaclyn S. (Jackie), HT(ASCP)) Date: Thu Mar 31 15:23:01 2011 Subject: [Histonet] Poll on B5 Message-ID: Hello all. I was asked by our director to try to come up with a list of places that do still use and do not still use B5. We are working hard on trying to get to be a mercury free facility and other than the B5 we use for bone marrows we are mercury free. So, if you all could be so kind as to send me a response that would be much appreciated. I would like to know what you currently use, if you used B5 in the past or if you are currently using B5 now, and hospital name. I would absolutely appreciate any and all comments and help you all can provide. Thank you! Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic, Rochester MN E-mail: pitts.jaclyn@mayo.edu From hlukey <@t> msn.com Thu Mar 31 15:28:24 2011 From: hlukey <@t> msn.com (Hugh Luk) Date: Thu Mar 31 15:28:29 2011 Subject: [Histonet] Biopsy Run Message-ID: Hi again Kim, >From your previous emails, it seems to be something your tissue processors cannot handle. Review your equipment and consumables. Below, is (almost) everything I think about when I hear "Some" tissues are staining/processing" irregularly: Formalin? Formalin-substitute? Recycled? Who makes your formalin? Some inconsistencies in vendor bulk formalin cubes have been noted. Processors? You said you have two. What types or brands? Dip-and-dunk (open) or infiltration chamber (closed)? If closed-type, when did your folks last clean or replace the tubing? Retort gasket? Cassette-type? Can your cassettes "Float" in the processor? Biopsy sponges used? http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-April/004782.html? Alcohol? Check with a Hydrometer? Who grades your alcohol? Recycled? Xylene or xylene-substitute? Recycled? Infiltrating Paraffin? Temperature? Confirm with a thermometer? Embedding - Temperature? Cassettes in holding tank for too long? Mictotomy - Soak your blocks in water/ice? Drying - How long in incubator? Temperature? As for your question about biopsy processing times, refer to Histotechnology A Self-Instructional Text, any edition, by Freida L. Carson, ASCP Press. In the 2nd edition pp 32-34 (note there is a troubleshooting portion too), Protocol #3 lists basically the processing run that I do overnight (and I think you do to), but I start in formalin. Everything is listed as 15 minutes, but it will also depend on what types of tissues are defined as "Biopsies," and you should extend the times as needed. I use 30 minutes, with some longer as listed. Pressure and vacuum is up to you, but I only use it throughout the paraffin steps. 10% alcohol 95% alcohol 95% alcohol 100% alcohol 100% alcohol (45 minutes) Xylene Xylene (45 minutes) Paraffin Paraffin Paraffin (45 minutes) Investigate these options and respond if needed. Hope this helps, Hugh-Hawaii > Date: Mon, 28 Mar 2011 12:32:32 -0800 > From: kkmarshall@anthc.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Biopsy Run > > Hello out there!!! > > Just a couple of quick questions...I wrote before with a "artifact" > problem and I thank everyone for their input. Bad thing is, I am still > having issues. > > Would my fellow Histo Techs please share with me the times you use on > your Biopsy Run's as well maybe give me some advise on biopsy cassettes. > I have used 2 different kind and just cant help but wonder if air > getting caught in the cassette may be one of my problems. > > Thanks in advance for your help...I sure need it. > > Kim > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSetlak <@t> childrensmemorial.org Thu Mar 31 15:33:00 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Thu Mar 31 15:34:04 2011 Subject: [Histonet] RE: Poll on B5 In-Reply-To: References: Message-ID: <7111DB39D045004C9CF29E79C71B28BC1018A80141@CMHEXCC01MBX.childrensmemorial.org> We still use it but are in the process of evaluating alternatives in order to phase it out. So far it looks like B-plus is winning. Lisa Children's Memorial Hospital- Chicago -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pitts, Jaclyn S. (Jackie), HT(ASCP) Sent: Thursday, March 31, 2011 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Poll on B5 Hello all. I was asked by our director to try to come up with a list of places that do still use and do not still use B5. We are working hard on trying to get to be a mercury free facility and other than the B5 we use for bone marrows we are mercury free. So, if you all could be so kind as to send me a response that would be much appreciated. I would like to know what you currently use, if you used B5 in the past or if you are currently using B5 now, and hospital name. I would absolutely appreciate any and all comments and help you all can provide. Thank you! Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic, Rochester MN E-mail: pitts.jaclyn@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu Mar 31 18:09:44 2011 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Mar 31 18:09:50 2011 Subject: [Histonet] Kappa/Lambda ISH on Ventana In-Reply-To: References: Message-ID: You should be able to get protocols directly from Ventana. I tried out their K and L ISH probes and they gave us the protocols. They also supplied us with the protocol we use for EBER ISH. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Wilkes Sent: Thursday, March 31, 2011 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa/Lambda ISH on Ventana Hi Histonet! I have been tasked with validating Kappa and Lambda on our Ventana Benchmark XT machines. Does anyone who uses this platform have suggestions as to which Ventana protease works best (1, 2 or 3)? Additionally, any other information concerning the optimization (suggested incubation times, RNA regions etc.) would be greatly appreciated. Thanks in advance. Steven srwilkes@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abeharry798 <@t> gmail.com Thu Mar 31 18:27:22 2011 From: abeharry798 <@t> gmail.com (Andrea) Date: Thu Mar 31 18:26:00 2011 Subject: [Histonet] Common requisition for Histology and Cytology Message-ID: Hi everyone, Does anyone use a common requisition for histology and cytology (and maybe flow too)? If yes would you be willing to share a copy of your requsition. If not do you know of any location who does? Thanks for your time, Andrea Beharry Technical Specialist Pathology Department William Osler Health System 2100 Bovaird Drive East Brampton, ON Canada L6R 3J7 * Andrea.Beharry@williamoslerhs.ca NEW