AW: [Histonet] Quenching

Gudrun Lang gu.lang <@t> gmx.at
Wed Jun 29 12:33:48 CDT 2011


Quenching is also a term used with depressing fluorescence activity. This
can happen in quantitative realtime-PCR procedures. Here a quencher is
situated close to a fluorochrom and therefore the activity is low. During
incorporation of the nucleotides the quencher is released and the
fluorochrom-activity is restored.

Gudrun Lang

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Candice
Smoots
Gesendet: Mittwoch, 29. Juni 2011 17:21
An: Histonet
Betreff: [Histonet] Quenching

Hi there my fellow histonetters!

I was wondering if anyone out there can tell me the purpose of quenching?
Thanks
 I remain yours truely,

Candice Camille
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