[Histonet] Pre-made paraformaldehyde question

[Sherwood, Margaret]

I failed to mention that I make up my Karnovsky's and freeze aliquots.  I have
never had a problem. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Suhyoung Jeong
Sent: Monday, June 06, 2011 8:39 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Pre-made paraformaldehyde question

Dear Histo people,

In my old lab, I have been taught that 4% PFA has to be always made fresh
(maybe a day before max). Here in my new lab, I find that people use 32%
pre-made PFA from EMS (without expiry date) and only dilute it on the day of
use.

Does anyone have experience (or thoughts) with this issue?

Thank you in advance

All the best, Suh
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