From sonya.martin <@t> soton.ac.uk Wed Jun 1 06:39:44 2011 From: sonya.martin <@t> soton.ac.uk (James S.) Date: Wed Jun 1 06:40:09 2011 Subject: [Histonet] Nuclear artefacts in FFPE tissues Message-ID: <5F338719C0D0DE44BDFDD2B83D3FF7A1CBAB2F163A@UOS-CL-EX7-L3.soton.ac.uk> I've been attempting to do some multi-colour fluorescence labelling of FFPE lymphoma tissue - usually I only use frozen tissue. It was all going smoothly on some FFPE tonsil sections but with the lymphoma tissues I see punctate nuclear staining with an epitope that should not be nuclear (CD32) - in some samples it appears as punctate staining all over the nuclei but in other samples it appears as a perinuclear halo. It only occurred with one particular antibody (rabbit anti-CD32) and not with rabbit anti-CD20 or mouse anti-CD68. Could this staining just be an artefact - is this common with FFPE tissue and is there something I can do to get rid of it. Thanks Sonya From Farnana <@t> nehealth.com Wed Jun 1 09:02:19 2011 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Wed Jun 1 09:02:29 2011 Subject: [Histonet] sentinel lymph node melanoma protocols Message-ID: <4DE60E2B.26ED.00D9.1@nehealth.com> Good morning everyone, I am curious to see what the standard protocol is on sentinel lymph node for melanomas. I need to establish a protocol at my institution and I have been reading a lot of literature and it seems to be a wide variety of thinking out there from bread loafing the lymph node first then cutting multiple H&E sections and IHC in between to bivalving. Some of the protocols call for up to as many as 46 H&E sections at 3 microns but it doesn't say where to take the sections for the IHC (S100, pan-melan cocktail). Would any of you like to share your protocols? thank you- Amy Farnan Histology Supervisor Northeast Health Albany, New York Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From dawudhill <@t> yahoo.com Wed Jun 1 11:40:03 2011 From: dawudhill <@t> yahoo.com (Dawud AlYasa) Date: Wed Jun 1 11:40:19 2011 Subject: [Histonet] Bone Marrow H&E Protocols Message-ID: <759774.67432.qm@web34506.mail.mud.yahoo.com> Hello Histonet! ? Does anyone have an H&E automated stainer protocol for bone marrows that they wouldn't mind sharing? I'm looking at different protocols. It would be greatly appreciated. Thanks. ? Dawud From algranth <@t> email.arizona.edu Wed Jun 1 11:54:01 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Jun 1 11:54:13 2011 Subject: [Histonet] pic of tattooed skin Message-ID: <55DCEF43-7DD7-4117-A62D-5790DA604224@email.arizona.edu> Good Morning! Found out a few days ago that next week we are participating in a summer camp for high schoolers and it was decided to use skin this year for our examples. I have lots of slides of skin sections from mice, rats, frogs, etc. and even some human skin punches - normal and with skin cancers from when we did a project for a sunscreen manufacturer. What I was looking for was an example of tattooed skin and I spent a bit of time searching for one yesterday online but couldn't find anything that was suitable. Does anyone out there have a picture of skin that has been tattooed? And maybe a good skin scar? Last year's camp was on liver, which was way easier! Thanks. Andi Grantham From mjdessoye <@t> wvhcs.org Wed Jun 1 12:00:35 2011 From: mjdessoye <@t> wvhcs.org (Dessoye, Michael J) Date: Wed Jun 1 12:00:40 2011 Subject: [Histonet] Keyboard and mouse covers Message-ID: Hi, We're upgrading our grossing areas with new workstations that require the techs to interact with a keyboard and mouse. I'd like to get keyboard and mouse covers to protect them from the usual hazards of grossing! But I can't seem to find any big vendors that sell them...does anyone use them and could recommend a vendor for these covers? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From LSetlak <@t> childrensmemorial.org Wed Jun 1 12:07:17 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Wed Jun 1 12:07:40 2011 Subject: [Histonet] RE: Keyboard and mouse covers In-Reply-To: References: Message-ID: <7111DB39D045004C9CF29E79C71B28BC101EF2B699@CMHEXCC01MBX.childrensmemorial.org> I just purchased a keyboard and mouse called Kleen Keys from American Mastertech and they make a washable mouse as well. Our PA is so-so about them...the keyboard is a little sensitive and the mouse is a little bigger than the standard size mouse. Hope this helps- see link below. Lisa http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=EQZ03BK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Wednesday, June 01, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Keyboard and mouse covers Hi, We're upgrading our grossing areas with new workstations that require the techs to interact with a keyboard and mouse. I'd like to get keyboard and mouse covers to protect them from the usual hazards of grossing! But I can't seem to find any big vendors that sell them...does anyone use them and could recommend a vendor for these covers? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Jun 1 12:15:45 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Jun 1 12:15:50 2011 Subject: [Histonet] RE: Keyboard and mouse covers In-Reply-To: References: Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640820E10C72@CHEXCMS10.one.ads.che.org> We use plastic wrap... Easy to change out and keep clean! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Wednesday, June 01, 2011 13:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Keyboard and mouse covers Hi, We're upgrading our grossing areas with new workstations that require the techs to interact with a keyboard and mouse. I'd like to get keyboard and mouse covers to protect them from the usual hazards of grossing! But I can't seem to find any big vendors that sell them...does anyone use them and could recommend a vendor for these covers? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From trathborne <@t> somerset-healthcare.com Wed Jun 1 12:18:43 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jun 1 12:19:40 2011 Subject: [Histonet] RE: Keyboard and mouse covers In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640820E10C72@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E1640820E10C72@CHEXCMS10.one.ads.che.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707013130@SMCMAIL01.somerset-healthcare.com> Do you completely wrap your mouse? Or do you leave the ball exposed? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, June 01, 2011 1:16 PM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Keyboard and mouse covers We use plastic wrap... Easy to change out and keep clean! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Wednesday, June 01, 2011 13:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Keyboard and mouse covers Hi, We're upgrading our grossing areas with new workstations that require the techs to interact with a keyboard and mouse. I'd like to get keyboard and mouse covers to protect them from the usual hazards of grossing! But I can't seem to find any big vendors that sell them...does anyone use them and could recommend a vendor for these covers? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From JWeems <@t> sjha.org Wed Jun 1 12:21:25 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Jun 1 12:21:30 2011 Subject: [Histonet] RE: Keyboard and mouse covers In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707013130@SMCMAIL01.somerset-healthcare.com> References: <92AD9B20A6C38C4587A9FEBE3A30E1640820E10C72@CHEXCMS10.one.ads.che.org> <3AD061FE740D464FAC7BF6B5CFB75707013130@SMCMAIL01.somerset-healthcare.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640820E10C77@CHEXCMS10.one.ads.che.org> Actually, we put the mouse in a baggie. But the whole thing is covered. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Wednesday, June 01, 2011 13:19 To: Weems, Joyce; Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: RE: Keyboard and mouse covers Do you completely wrap your mouse? Or do you leave the ball exposed? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, June 01, 2011 1:16 PM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Keyboard and mouse covers We use plastic wrap... Easy to change out and keep clean! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Wednesday, June 01, 2011 13:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Keyboard and mouse covers Hi, We're upgrading our grossing areas with new workstations that require the techs to interact with a keyboard and mouse. I'd like to get keyboard and mouse covers to protect them from the usual hazards of grossing! But I can't seem to find any big vendors that sell them...does anyone use them and could recommend a vendor for these covers? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From eridana <@t> cox.net Wed Jun 1 13:11:38 2011 From: eridana <@t> cox.net (Eridana) Date: Wed Jun 1 13:11:43 2011 Subject: [Histonet] Trichrome stain on frozens In-Reply-To: <20110601170154.IOEA9002.eastrmfepi104.cox.net@eastrmimpi05.cox.net> Message-ID: <20110601141138.POWI2.691059.imail@fed1rmwml43> I had major problems with frozen trichrome years ago and Jamie Watson from GNF, La Jolla said to fix in the Bouins and not fix in formalin and then mordant in Bouins. (duh) I am not sure why I never tried that, but when I fixed in Bouins for 10 minutes I got consistent trichrome on frozen skin sections. Donna Harclerode, HT,HTL (ASCP) QIHC, SLS Arista Molecular Immunohistochemist 10455 Pacific Center Court San Diego, CA 92121 858-866-5421 Donna.Harclerode@aristamolecular.com From histotech <@t> imagesbyhopper.com Wed Jun 1 13:31:25 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Jun 1 13:31:48 2011 Subject: [Histonet] RE: Keyboard and mouse covers In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E1640820E10C77@CHEXCMS10.one.ads.che.org> References: <92AD9B20A6C38C4587A9FEBE3A30E1640820E10C72@CHEXCMS10.one.ads.che.org> <3AD061FE740D464FAC7BF6B5CFB75707013130@SMCMAIL01.somerset-healthcare.com> <92AD9B20A6C38C4587A9FEBE3A30E1640820E10C77@CHEXCMS10.one.ads.che.org> Message-ID: For the keyboard, what about using the silicon style? A lab near me uses them exclusively. They are easy to keep clean, as they basically watertight - just wipe down with a sani-wipe! And, they are fairly inexpensive - I got one from Office Max (or was it Staples?) for $10. Sent from my iPhone On Jun 1, 2011, at 1:21 PM, "Weems, Joyce" wrote: > Actually, we put the mouse in a baggie. But the whole thing is covered. > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > -----Original Message----- > From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] > Sent: Wednesday, June 01, 2011 13:19 > To: Weems, Joyce; Dessoye, Michael J; histonet@lists.utsouthwestern.edu > Subject: RE: Keyboard and mouse covers > > Do you completely wrap your mouse? Or do you leave the ball exposed? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce > Sent: Wednesday, June 01, 2011 1:16 PM > To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Keyboard and mouse covers > > We use plastic wrap... Easy to change out and keep clean! > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J > Sent: Wednesday, June 01, 2011 13:01 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Keyboard and mouse covers > > Hi, > > We're upgrading our grossing areas with new workstations that require the techs to interact with a keyboard and mouse. I'd like to get keyboard and mouse covers to protect them from the usual hazards of grossing! But I can't seem to find any big vendors that sell them...does anyone use them and could recommend a vendor for these covers? Thanks! > > Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | > 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. > If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. > > Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond <@t> gmail.com Wed Jun 1 14:03:03 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Jun 1 14:03:07 2011 Subject: [Histonet] Re: re-using modified Davidson's solution Message-ID: Robin Dean asks: >>Can one re-use modified Davidson's solution for fixing eyes and testes, or has it lost its potency/efficacy after being used previously? Can you rejuvenate it by adding more acetic acid? (how much?)<< I would think that limited re-use would be acceptable - the proportions of the ingredients in the formula (3 parts water, 3 parts alcohol, 2 parts 37% formalin, 1 part glacial acetic acid) are pretty forgiving. I wouldn't "rejuvenate" it. Bob Richmond Samurai Pathologist Knoxville TN From ROrr <@t> northshore.org Wed Jun 1 14:35:56 2011 From: ROrr <@t> northshore.org (Orr, Rebecca) Date: Wed Jun 1 14:36:00 2011 Subject: [Histonet] Melanoma sentinel nodes In-Reply-To: <661928f4-8eb3-4ef1-9b96-ae1b30b8b888@EXCHCAS01.enhnet.org> References: <661928f4-8eb3-4ef1-9b96-ae1b30b8b888@EXCHCAS01.enhnet.org> Message-ID: Amy, I can't find the exact reference but I think either Balch or Taylor authored the paper that was adapted for our protocol. Level 1, 3, 5, 10 are H/E and extra section at level 5 is taken for Negative serum control Level 2 is S100 Level 4, 6 is PanMelanoma cocktail (Biocare Medical) Level 7, 8, 9, are held for as unstained and filed away additional H/E or IHC We usually aim for about 200 microns in between levels. This is entirely dependent on the degree of adipose tissue that was included around the node and the thickness of the node in the paraffin block. When we started this protocol about 5 years ago, we would get 1 or 2 paraffin blocks for analysis. Nowadays we typically receive 3-6 blocks per case (sometimes 2 cases per week) and this has impacted our workload. Hope this helps. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 From: "Amy Farnan" Subject: [Histonet] sentinel lymph node melanoma protocols To: Message-ID: <4DE60E2B.26ED.00D9.1@nehealth.com> Content-Type: text/plain; charset=US-ASCII Good morning everyone, I am curious to see what the standard protocol is on sentinel lymph node for melanomas. I need to establish a protocol at my institution and I have been reading a lot of literature and it seems to be a wide variety of thinking out there from bread loafing the lymph node first then cutting multiple H&E sections and IHC in between to bivalving. Some of the protocols call for up to as many as 46 H&E sections at 3 microns but it doesn't say where to take the sections for the IHC (S100, pan-melan cocktail). Would any of you like to share your protocols? thank you- Amy Farnan Histology Supervisor Northeast Health Albany, New York Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. ------------------------------ Message: 7 Date: Wed, 1 Jun 2011 09:40:03 -0700 (PDT) From: Dawud AlYasa Subject: [Histonet] Bone Marrow H&E Protocols To: histonet@lists.utsouthwestern.edu Message-ID: <759774.67432.qm@web34506.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello Histonet! ? Does anyone have an H&E automated stainer protocol for bone marrows that they wouldn't mind sharing? I'm looking at different protocols. It would be greatly appreciated. Thanks. ? Dawud ------------------------------ Message: 8 Date: Wed, 1 Jun 2011 09:54:01 -0700 From: "Grantham, Andrea L - (algranth)" Subject: [Histonet] pic of tattooed skin To: HISTONET Message-ID: <55DCEF43-7DD7-4117-A62D-5790DA604224@email.arizona.edu> Content-Type: text/plain; charset="us-ascii" Good Morning! Found out a few days ago that next week we are participating in a summer camp for high schoolers and it was decided to use skin this year for our examples. I have lots of slides of skin sections from mice, rats, frogs, etc. and even some human skin punches - normal and with skin cancers from when we did a project for a sunscreen manufacturer. What I was looking for was an example of tattooed skin and I spent a bit of time searching for one yesterday online but couldn't find anything that was suitable. Does anyone out there have a picture of skin that has been tattooed? And maybe a good skin scar? Last year's camp was on liver, which was way easier! Thanks. Andi Grantham ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 91, Issue 1 *************************************** Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. From mwhite <@t> mcleodhealth.org Wed Jun 1 14:59:04 2011 From: mwhite <@t> mcleodhealth.org (mwhite@mcleodhealth.org) Date: Wed Jun 1 14:59:07 2011 Subject: [Histonet] pathology intradepartmental consults Message-ID: Histonetters: What percentage of anatomical pathology cases in your facility are reviewed by a second pathologist (intradepartmental consult)? Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmaster@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. From rjbuesa <@t> yahoo.com Wed Jun 1 15:06:31 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 1 15:06:36 2011 Subject: [Histonet] pathology intradepartmental consults In-Reply-To: References: Message-ID: <249184.94365.qm@web65712.mail.ac4.yahoo.com> Around 10% (mostly requested by junior partners). Ren? J. From: "mwhite@mcleodhealth.org" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 1, 2011 3:59 PM Subject: [Histonet] pathology intradepartmental consults Histonetters: What percentage of anatomical pathology cases in your facility are reviewed by a second pathologist (intradepartmental consult)? Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmaster@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Jun 1 15:12:44 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jun 1 15:17:43 2011 Subject: [Histonet] printed requisitions Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707013287@SMCMAIL01.somerset-healthcare.com> Does everyone still receive printed requisitions with your surgical specimens? How many of your OR's have a printer in each room? Our OR has a shared printer for all of the procedure rooms, but would like to have a printer for each OR suite. Our IT department is saying there would be problems and has suggested that we go paperless. I see many problems arising from this. If anyone is doing this successfully, I'd be very interested in hearing how you accomplished it. Toni Regards, Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville, NJ 08876 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From amber.mckenzie <@t> gastrodocs.net Wed Jun 1 15:31:32 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Jun 1 15:31:19 2011 Subject: [Histonet] Fume hood In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707013287@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB75707013287@SMCMAIL01.somerset-healthcare.com> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B37042B28FD@giamail2.Gia.com> What are you using to check your fume hood's with for QC purposes? From liz <@t> premierlab.com Wed Jun 1 15:35:25 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jun 1 15:35:29 2011 Subject: [Histonet] Fume hood In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B37042B28FD@giamail2.Gia.com> Message-ID: We have a service come out and inspect our hoods once a year. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, June 01, 2011 2:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fume hood What are you using to check your fume hood's with for QC purposes? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jun 1 15:40:46 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 1 15:40:48 2011 Subject: [Histonet] Fume hood In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B37042B28FD@giamail2.Gia.com> References: <3AD061FE740D464FAC7BF6B5CFB75707013287@SMCMAIL01.somerset-healthcare.com> <03C921A1EAF7F541B16543F6EC6A4B37042B28FD@giamail2.Gia.com> Message-ID: <124504.66905.qm@web65716.mail.ac4.yahoo.com> We installed small vanes in the area of maximum flow?inside the hood and we daily checked that the vanes were moving by the air. As to the actual flow in f/min the hoods were checked annually by an outside company. Ren? J. From: Amber McKenzie To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 1, 2011 4:31 PM Subject: [Histonet] Fume hood What are you using to check your fume hood's with for QC purposes? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Jun 1 15:46:24 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Jun 1 15:46:28 2011 Subject: [Histonet] RE: printed requisitions In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707013287@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB75707013287@SMCMAIL01.somerset-healthcare.com> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640820E10D4E@CHEXCMS10.one.ads.che.org> We still use paper. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, June 01, 2011 16:13 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] printed requisitions Does everyone still receive printed requisitions with your surgical specimens? How many of your OR's have a printer in each room? Our OR has a shared printer for all of the procedure rooms, but would like to have a printer for each OR suite. Our IT department is saying there would be problems and has suggested that we go paperless. I see many problems arising from this. If anyone is doing this successfully, I'd be very interested in hearing how you accomplished it. Toni Regards, Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville, NJ 08876 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Candy.A.Bales <@t> uth.tmc.edu Wed Jun 1 16:05:02 2011 From: Candy.A.Bales <@t> uth.tmc.edu (Bales, Candy A) Date: Wed Jun 1 16:05:08 2011 Subject: [Histonet] Leica processor Message-ID: <62C915811DD5A142851D95CA6BC5D1E41B39CCDB5E@UTHCMS1.uthouston.edu> On behalf of a friend who is looking for a new tissue processor. She is asking for information on a Leica ASB300. Any information would be appreciated. Also, any pros & cons of a long term lease/option to buy? Thank you Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax From Gina.Rodriguez <@t> leica-microsystems.com Wed Jun 1 16:19:54 2011 From: Gina.Rodriguez <@t> leica-microsystems.com (Gina.Rodriguez@leica-microsystems.com) Date: Wed Jun 1 16:20:07 2011 Subject: [Histonet] Gina Rodriguez is out of the office. Message-ID: I will be out of the office starting 06/01/2011 and will not return until 06/06/2011. I will respond to your message when I return. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From tjrichmond <@t> hughes.net Wed Jun 1 16:28:47 2011 From: tjrichmond <@t> hughes.net (Tonia Richmond) Date: Wed Jun 1 16:28:52 2011 Subject: [Histonet] Slide Volume Inquiry Message-ID: <2041239713.463998.1306963727386.JavaMail.mail@webmail07> I'm trying to determine the annual volume of glass slides produced globally for a project I'm working on. I'm having no luck finding any information. Any suggestions would be greatly appreciated!! All the best, Tj Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From Candy.A.Bales <@t> uth.tmc.edu Wed Jun 1 16:30:25 2011 From: Candy.A.Bales <@t> uth.tmc.edu (Bales, Candy A) Date: Wed Jun 1 16:30:29 2011 Subject: [Histonet] FW: Leica processor Message-ID: <62C915811DD5A142851D95CA6BC5D1E41B39CCDB69@UTHCMS1.uthouston.edu> Sorry, its an ASP300 processor. Thanks From: Bales, Candy A Sent: Wednesday, June 01, 2011 4:05 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Leica processor On behalf of a friend who is looking for a new tissue processor. She is asking for information on a Leica ASB300. Any information would be appreciated. Also, any pros & cons of a long term lease/option to buy? Thank you Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax From Dishop.Megan <@t> tchden.org Wed Jun 1 17:30:45 2011 From: Dishop.Megan <@t> tchden.org (Dishop, Megan) Date: Wed Jun 1 17:30:49 2011 Subject: [Histonet] pathology intradepartmental consults In-Reply-To: References: Message-ID: <0DF924EDD3F9C248A18538F87E738823032E7B5B@TCHEXMBCCR5.thechildrenshospital.org> Internal consults are in the range of 2-7% in our institution. Some of these are due to mandated second review of all new malignancies. Retrospective review of cases by other pathologists, for example conferences/tumor board/etc, is higher, in the range of 10-14% of cases. Megan K. Dishop MD Department of Pathology, B120 The Children's Hospital 13123 E. 16th Avenue Aurora, CO 80045 Ph: 720-777-4337 Fax: 720-777-7119 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mwhite@mcleodhealth.org Sent: Wednesday, June 01, 2011 1:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pathology intradepartmental consults Histonetters: What percentage of anatomical pathology cases in your facility are reviewed by a second pathologist (intradepartmental consult)? Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmaster@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail is confidential, may be legally privileged, and for the intended recipient only. Access, disclosure, copying, forwarding and distribution by any means is strictly prohibited. If received in error, do not read but delete and e-mail confirmation to the sender. ================================================================================ From rsrichmond <@t> gmail.com Wed Jun 1 17:48:01 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Jun 1 17:48:05 2011 Subject: [Histonet] Re: pathology intradepartmental consults Message-ID: Melanie S. White, MT(ASCP), Laboratory Supervisor, Systems/Anatomic Pathology, McLeod Regional Medical Center asks: >>What percentage of anatomical pathology cases in your facility are reviewed by a second pathologist (intradepartmental consult)?<< The one group I've worked with that addressed this question was a group of five experienced surgical pathologists who kept very careful records of their internal consults. They maintained an intradepartmental consultation rate of 2.6%, which they thought was optimal for their particular practice situation. Bob Richmond Samurai Pathologist Knoxville TN From Diane.Tokugawa <@t> kp.org Wed Jun 1 18:28:55 2011 From: Diane.Tokugawa <@t> kp.org (Diane.Tokugawa@kp.org) Date: Wed Jun 1 18:29:49 2011 Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting 06/01/2011 and will not return until 06/07/2011. Note: For Cytology issues, please call Molly (day) at 8-421-5487 or Eric (eve) at 8-421-5405, For Histology issues, please call the general histology lab 8-421- 5408, Mario 8-421-4961 (day), Kiran 8-421-5404 (late afternoon/eve) or Wanda Lau for Cyto/Histo issues 8-421-5426. From szigcs <@t> bio.u-szeged.hu Thu Jun 2 01:44:13 2011 From: szigcs <@t> bio.u-szeged.hu (szigcs@bio.u-szeged.hu) Date: Thu Jun 2 01:44:45 2011 Subject: [Histonet] Lipofuscin and counterstaining DAb IHC Message-ID: <20110602084413.sybban6dwsc08gc4@webmail.u-szeged.hu> Dear Histonetters! I have human brain samples (age 70, HUntington disease) and my goal is to quantitate changes in the number of neurons/glia cells with nuclei staining. I use NeuN IHC and H?echst. My first question is: - Throughout the whole sample there are a lot of lipofuscin signal fading the NeuN signal, we are not able to make any microphtograph for cell counting. I tried Sudan black staining and Sigma autofluorescent reagent with the same result. Could you advise some tips and tricks to avoid this? An alternative solution could be DAB IHC for NeuN and counterstaining with nucleus staining. Second question is: - what type of counterstain do you recommend to be able to identify the IHC and normal nucleus staining in the same section? Thank you in advance, best regards Csaba ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From AnthonyH <@t> chw.edu.au Thu Jun 2 01:51:46 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jun 2 01:52:13 2011 Subject: [Histonet] Lipofuscin and counterstaining DAb IHC In-Reply-To: <20110602084413.sybban6dwsc08gc4@webmail.u-szeged.hu> References: <20110602084413.sybban6dwsc08gc4@webmail.u-szeged.hu> Message-ID: <6D6BD1DE8A5571489398B392A38A715718858D99@xmdb02.nch.kids> Csaba, I can't answer the fluorescent question but for light microscopic IHC I would stain for the NeuN using peroxidase-DAB followed by a PAS for lipofuscin followed by Mayer's or Harris's haematoxylin. Result is Brown, pink, blue Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of szigcs@bio.u-szeged.hu Sent: Thursday, 2 June 2011 4:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lipofuscin and counterstaining DAb IHC Dear Histonetters! I have human brain samples (age 70, HUntington disease) and my goal is to quantitate changes in the number of neurons/glia cells with nuclei staining. I use NeuN IHC and H?echst. My first question is: - Throughout the whole sample there are a lot of lipofuscin signal fading the NeuN signal, we are not able to make any microphtograph for cell counting. I tried Sudan black staining and Sigma autofluorescent reagent with the same result. Could you advise some tips and tricks to avoid this? An alternative solution could be DAB IHC for NeuN and counterstaining with nucleus staining. Second question is: - what type of counterstain do you recommend to be able to identify the IHC and normal nucleus staining in the same section? Thank you in advance, best regards Csaba ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From k84as <@t> yahoo.com Thu Jun 2 02:53:19 2011 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Thu Jun 2 02:53:27 2011 Subject: [Histonet] Alcian blue quistion Message-ID: <220877.89758.qm@web112611.mail.gq1.yahoo.com> hi every body i have a confusing quistion reagrding Alcian blue pH 1 & 2.5 stains. suppose you have 100 goblet cell react positive to pH 1 which mean all have sulfated mucin. and 50 cell from other section react positive to pH 2.5 which mean acidic type(sulfated or sialated). why these 50 cell only react to pH 2.5 althogh the other non reacted cells possess acidic sulfated type?? on other word i need to know for what type of mucins alcian B 2.5 react with? ? thanx all From j.brinker <@t> att.net Thu Jun 2 05:03:42 2011 From: j.brinker <@t> att.net (Jean Brinker) Date: Thu Jun 2 05:03:48 2011 Subject: [Histonet] nuclear haziness Message-ID: <207250.74842.qm@web180314.mail.gq1.yahoo.com> We are having an issue with nuclear haziness on prostate biopsies in our small urology laboratory.? It occurs randomly,?appearing on some levels and not others within the same block. Another scenario is that we sometimes see it occur only in one small area of a core and the remaining tissue in the core looks beautiful. It can occur throughout a case (12 blocks) or not at all. We have checked for obvious problems, i.e. reagent contamination on the processor and stainer, ruled out?temperature issues, microtome problems, etc. to no avail. I would appreciate any input/suggestions that would help to alleviate this problem. Thank you, Jean Brinker St. Louis Urological Surgeons St. Peters, MO From TMcNemar <@t> lmhealth.org Thu Jun 2 05:11:30 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Jun 2 05:11:37 2011 Subject: [Histonet] RE: printed requisitions In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707013287@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB75707013287@SMCMAIL01.somerset-healthcare.com> Message-ID: We still require a paper req with all specimens. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, June 01, 2011 4:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] printed requisitions Does everyone still receive printed requisitions with your surgical specimens? How many of your OR's have a printer in each room? Our OR has a shared printer for all of the procedure rooms, but would like to have a printer for each OR suite. Our IT department is saying there would be problems and has suggested that we go paperless. I see many problems arising from this. If anyone is doing this successfully, I'd be very interested in hearing how you accomplished it. Toni Regards, Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville, NJ 08876 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From tgenade <@t> gmail.com Thu Jun 2 06:50:26 2011 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Thu Jun 2 06:50:31 2011 Subject: [Histonet] question about Bielschowsky's Silver Stain results Message-ID: Hello, I have question regarding results I got using the Bielschowsky's Silver Stain for plaques and tangles. The images and questions pertaining to them are up at http://www.ihcworld.com/smf/index.php/topic,6236.0.html . I would greatly appreciate the input from some one who has used this stain before. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From HornHV <@t> archildrens.org Thu Jun 2 07:11:03 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Jun 2 07:11:07 2011 Subject: [Histonet] RE: Leica processor In-Reply-To: <62C915811DD5A142851D95CA6BC5D1E41B39CCDB69@UTHCMS1.uthouston.edu> References: <62C915811DD5A142851D95CA6BC5D1E41B39CCDB69@UTHCMS1.uthouston.edu> Message-ID: <25A4DE08332B19499904459F00AAACB71987E03D7C@EVS1.archildrens.org> We have one and it's been reliable. It's about 5-6 years old. It's also easy to use. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bales, Candy A Sent: Wednesday, June 01, 2011 4:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Leica processor Sorry, its an ASP300 processor. Thanks From: Bales, Candy A Sent: Wednesday, June 01, 2011 4:05 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Leica processor On behalf of a friend who is looking for a new tissue processor. She is asking for information on a Leica ASB300. Any information would be appreciated. Also, any pros & cons of a long term lease/option to buy? Thank you Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From HornHV <@t> archildrens.org Thu Jun 2 07:18:20 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Jun 2 07:18:24 2011 Subject: [Histonet] RE: printed requisitions In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707013287@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB75707013287@SMCMAIL01.somerset-healthcare.com> Message-ID: <25A4DE08332B19499904459F00AAACB71987E03D7E@EVS1.archildrens.org> We still use paper reqs. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, June 01, 2011 3:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] printed requisitions Does everyone still receive printed requisitions with your surgical specimens? How many of your OR's have a printer in each room? Our OR has a shared printer for all of the procedure rooms, but would like to have a printer for each OR suite. Our IT department is saying there would be problems and has suggested that we go paperless. I see many problems arising from this. If anyone is doing this successfully, I'd be very interested in hearing how you accomplished it. Toni Regards, Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville, NJ 08876 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From kkmarshall <@t> anthc.org Thu Jun 2 08:56:39 2011 From: kkmarshall <@t> anthc.org (Marshall, Kimberly K) Date: Thu Jun 2 09:04:33 2011 Subject: [Histonet] Counter top immunos Message-ID: Hello Histo folks Hope everyone is having a great week....I am writing to get some info on immuno kits for staining just by hand, like back when I first started in this field. We are a small lab in Alaska and all of our immunos are sent out of state, We are looking to do some of them here and try to save money, but don't really need a stainer. So are there any kits that are better than others? Is there anyone that does immunos this way that would give some much needed advise??? Again thanks in advance for all the imput... Kimberly From araniqkslvr <@t> yahoo.com Thu Jun 2 09:20:50 2011 From: araniqkslvr <@t> yahoo.com (Paula) Date: Thu Jun 2 09:20:54 2011 Subject: [Histonet] Looking for Part Time Job Message-ID: <964634.50249.qm@web30302.mail.mud.yahoo.com> Hello, ? I am looking for a part time job to learn histology in the Raleigh/Durham area of North Carolina. Maybe a few hours a week on Saturday or something like that. I am HT registered. ? Paula From TGoins <@t> mt.gov Thu Jun 2 09:32:05 2011 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Jun 2 09:32:11 2011 Subject: [Histonet] RE: Counter top immunos In-Reply-To: References: Message-ID: We also do our immunos manually - but we use no kits. I do not promote but do suggest: - Biocare Vulcan Fast Red as chromogen - air dry slide after counterstaining with hematoxylin, then coverslip - KPL for linkers - concentrated antibodies; ready-to-use are good to check to see if the assay works, but shelf-life is longer if concentrated Good luck and have fun! Tresa Goins Histopathology Supervisor Department of Livestock Bozeman, Montana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Thursday, June 02, 2011 7:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Counter top immunos Hello Histo folks Hope everyone is having a great week....I am writing to get some info on immuno kits for staining just by hand, like back when I first started in this field. We are a small lab in Alaska and all of our immunos are sent out of state, We are looking to do some of them here and try to save money, but don't really need a stainer. So are there any kits that are better than others? Is there anyone that does immunos this way that would give some much needed advise??? Again thanks in advance for all the imput... Kimberly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jun 2 09:46:04 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 2 09:46:07 2011 Subject: [Histonet] Slide Volume Inquiry In-Reply-To: <2041239713.463998.1306963727386.JavaMail.mail@webmail07> References: <2041239713.463998.1306963727386.JavaMail.mail@webmail07> Message-ID: <382222.56867.qm@web65706.mail.ac4.yahoo.com> How are you going to do that? Do you know how many laboratories there are? Do you know the average slides/year for each? Unless you know both parameters you cannot calculate the total "national" volume. I wish you luck! Ren? J. From: Tonia Richmond To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 1, 2011 5:28 PM Subject: [Histonet] Slide Volume Inquiry ? I'm? trying? to? determine? the annual volume of glass slides produced ? globally for a project I'm working on.? I'm having no luck finding any ? information.? Any suggestions would be greatly appreciated!! ? All the best, ? Tj ? Confidentiality? Notice:? This? e-mail? message,? including? any ? attachments,? is for the sole use of the intended recipient(s) and may ? contain? confidential? and? privileged? information.? Any unauthorized ? review, use, disclosure or distribution is prohibited.? If you are not ? the? intended recipient, please contact the sender by reply e-mail and ? destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jun 2 09:50:08 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 2 09:50:21 2011 Subject: [Histonet] nuclear haziness In-Reply-To: <207250.74842.qm@web180314.mail.gq1.yahoo.com> References: <207250.74842.qm@web180314.mail.gq1.yahoo.com> Message-ID: <687095.54911.qm@web65713.mail.ac4.yahoo.com> Make sure that ALL your slides are properly dried in the oven and that NONE has any remnant of water underneath before going into the oven to dry. Ren? J. From: Jean Brinker To: histonet@lists.utsouthwestern.edu Sent: Thursday, June 2, 2011 6:03 AM Subject: [Histonet] nuclear haziness We are having an issue with nuclear haziness on prostate biopsies in our small urology laboratory.? It occurs randomly,?appearing on some levels and not others within the same block. Another scenario is that we sometimes see it occur only in one small area of a core and the remaining tissue in the core looks beautiful. It can occur throughout a case (12 blocks) or not at all. We have checked for obvious problems, i.e. reagent contamination on the processor and stainer, ruled out?temperature issues, microtome problems, etc. to no avail. I would appreciate any input/suggestions that would help to alleviate this problem. Thank you, Jean Brinker St. Louis Urological Surgeons St. Peters, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arme <@t> optonline.net Thu Jun 2 10:37:24 2011 From: arme <@t> optonline.net (American ReSource Medical) Date: Thu Jun 2 10:37:30 2011 Subject: [Histonet] Available for sale - Countertop Grossing Station Message-ID: (1) Mopec MB100 Grossing Station http://media2.mopec.com/media/pdf/MBSeriesGrossingStationBrochure.pdf Excellent condition. Mark Sofferman, President American ReSource Medical 324 West Englewood Avenue Teaneck, NJ 07666 P: 201.833.1550 F: 201.833.1575 From becky.garrison <@t> jax.ufl.edu Thu Jun 2 10:38:45 2011 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Thu Jun 2 10:38:50 2011 Subject: [Histonet] Slide Volume Inquiry In-Reply-To: <2041239713.463998.1306963727386.JavaMail.mail@webmail07> References: <2041239713.463998.1306963727386.JavaMail.mail@webmail07> Message-ID: <9E47DE9D490DCC42A2EAE94F22BF93F202E6A5C4@JXB10-MAIL3.umc.ufl.edu> There is an article on histology workload in the current issue of: Arch Pathol Lab Med Vol 135, June 2011 Just placed on my desk today; I have only glanced at it. It may have some information helpful to you. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tonia Richmond Sent: Wednesday, June 01, 2011 5:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Volume Inquiry I'm trying to determine the annual volume of glass slides produced globally for a project I'm working on. I'm having no luck finding any information. Any suggestions would be greatly appreciated!! All the best, Tj Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Carmen.M.Garcia <@t> uv.es Thu Jun 2 10:54:37 2011 From: Carmen.M.Garcia <@t> uv.es (Carmen Maria Garcia Pascual) Date: Thu Jun 2 10:53:12 2011 Subject: [Histonet] filopodia of Endothelial cells in corpus luteum in mouse ovary Message-ID: <9714182188carmaga6@uv.es> Dear Histonet members: I am interesting in see the filopodia of the endothelial cells in the corpus luteum of mouse. I have stained 25um cryostat sections of ovary with an antibody agaisnt CD31 (PECAM) and alexa fluor and see it in the confocal microscope, but I haven't succes. someone can tell me what can I do? than you so much!!!!!! ******************************************************* Carmen Mar?a Garc?a Pascual FIVI/INCLIVA/Facultad de Medicina Universidad de Valencia Departamento de P.O.G (Laboratorios) 96.386.40.48 Avd. Blasco Iba?ez 17 46010, Valencia ******************************************************* From Ronald.Houston <@t> nationwidechildrens.org Thu Jun 2 11:09:03 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Jun 2 11:09:11 2011 Subject: [Histonet] PA traveler needed Message-ID: Would any recruiter who has a traveling PA available for 8-10 weeks coverage starting August please contact me off-line? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From asmith <@t> mail.barry.edu Thu Jun 2 13:05:30 2011 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu Jun 2 13:05:40 2011 Subject: [Histonet] RE: Counter top immunos In-Reply-To: References: Message-ID: I like the Vector ABC horseradish peroxidase kits, especially with the Nova Red chromogen. Mouse primary antibodies usually give me better results than rabbit or goat primaries. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Thursday, June 02, 2011 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Counter top immunos Hello Histo folks Hope everyone is having a great week....I am writing to get some info on immuno kits for staining just by hand, like back when I first started in this field. We are a small lab in Alaska and all of our immunos are sent out of state, We are looking to do some of them here and try to save money, but don't really need a stainer. So are there any kits that are better than others? Is there anyone that does immunos this way that would give some much needed advise??? Again thanks in advance for all the imput... Kimberly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Thu Jun 2 13:05:28 2011 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Jun 2 13:11:29 2011 Subject: [Histonet] laminin and collagen4 in cat and dog Message-ID: We want to use laminin and Collagen IV to sort out the origin of some spindle cell tumors. Specifically, malignant peripheral nerve sheath tumors reportedly show laminin and collagen IV basement membranes vs. Fibrosarcoma which does not. We need antibodies that will work in both cat and dog. Is there anyone who has had success with these? Do you have any recommendations for these antibodies? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From liz <@t> premierlab.com Thu Jun 2 13:27:00 2011 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jun 2 13:27:04 2011 Subject: [Histonet] RE: [IHCRG] laminin and collagen4 in cat and dog In-Reply-To: Message-ID: Margaret We have used the Collagen IV listed below on canine but not feline, for laminin we have not used the antibody from abcam on canine or feline but the spec sheet states it works on most mammals. Good Luck Liz Collagen IV SouternBio 0340-01 UNLB Goat Laminin abcam ab11575 Rabbit Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Perry, Margaret Sent: Thursday, June 02, 2011 12:05 PM To: ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: [IHCRG] laminin and collagen4 in cat and dog We want to use laminin and Collagen IV to sort out the origin of some spindle cell tumors. Specifically, malignant peripheral nerve sheath tumors reportedly show laminin and collagen IV basement membranes vs. Fibrosarcoma which does not. We need antibodies that will work in both cat and dog. Is there anyone who has had success with these? Do you have any recommendations for these antibodies? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. From hymclab.hymclab <@t> ministryhealth.org Thu Jun 2 13:50:43 2011 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Thu Jun 2 13:50:52 2011 Subject: [Histonet] RE: Counter top immunos In-Reply-To: References: Message-ID: We do ours manually also (about 20 different antibodies). We use a combo of BioGenex and Dako. I like both set ups, but Dako is more expensive to perform than BioGenex. I like that you can buy the in dividual components from BioGenex instead of a whole ket from DAKO. Dawn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Thursday, June 02, 2011 1:06 PM To: Marshall, Kimberly K Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Counter top immunos I like the Vector ABC horseradish peroxidase kits, especially with the Nova Red chromogen. Mouse primary antibodies usually give me better results than rabbit or goat primaries. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Thursday, June 02, 2011 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Counter top immunos Hello Histo folks Hope everyone is having a great week....I am writing to get some info on immuno kits for staining just by hand, like back when I first started in this field. We are a small lab in Alaska and all of our immunos are sent out of state, We are looking to do some of them here and try to save money, but don't really need a stainer. So are there any kits that are better than others? Is there anyone that does immunos this way that would give some much needed advise??? Again thanks in advance for all the imput... Kimberly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From rosenfeldtek <@t> hotmail.com Thu Jun 2 18:54:29 2011 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Thu Jun 2 18:54:32 2011 Subject: [Histonet] Alternatives to Cytospin? Message-ID: I will be collecting bronchoalveolar lavage fluid (BALF) from mice and want to do do a diff-quick stain on the fluid to characterize the leukocyte population. My colleagues usually use a cytospin to concentrate cells onto a slide but I'll be at a lab that doesn't have the equipment so I'm looking for alternatives. Could I just put a drop of the BALF onto a charged slide and air dry it? Or treat the BALF like a blood smear? Alternatively, could I fix the cells in...ethanol maybe and then cytospin them the next day when I get back to my lab? Any ideas will be much appreciated. Jerry Ricks Research Scientist University of Washington Department of Pathology From rosenfeldtek <@t> hotmail.com Thu Jun 2 19:03:46 2011 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Thu Jun 2 19:03:52 2011 Subject: [Histonet] RE: Counter top Immunos... Message-ID: For mouse and rabbit primary antibodies I like the Vector ImmPRESS kits. They save time. For HRP substrates Nova Red looks too dull to me. I have had good results with AEC and also with Vector ImmPACT DAB. Jerry Ricks Research Scientist University of Washington Department of Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Thursday, June 02, 2011 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Counter top immunos Hello Histo folks Hope everyone is having a great week....I am writing to get some info on immuno kits for staining just by hand, like back when I first started in this field. We are a small lab in Alaska and all of our immunos are sent out of state, We are looking to do some of them here and try to save money, but don't really need a stainer. So are there any kits that are better than others? Is there anyone that does immunos this way that would give some much needed advise??? Again thanks in advance for all the imput... Kimberly From AnthonyH <@t> chw.edu.au Thu Jun 2 19:41:23 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jun 2 19:41:35 2011 Subject: [Histonet] Alternatives to Cytospin? In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A7157188590A3@xmdb02.nch.kids> Jerry, Firstly, I would not recommend fixing in ethanol since cells will shrink and Romanowsky staining will be compromised (the cells stain too darkly since they are smaller). I would use a centrifuge to concentrate the cells and smear like a blood film or if you do not have a centrifuge available, place a drop of the BALF on the slide and either air dry as you suggested (if cell count is low) or treat it like blood (if it looks quite opaque - lots of cells/ & other junk). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Friday, 3 June 2011 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alternatives to Cytospin? I will be collecting bronchoalveolar lavage fluid (BALF) from mice and want to do do a diff-quick stain on the fluid to characterize the leukocyte population. My colleagues usually use a cytospin to concentrate cells onto a slide but I'll be at a lab that doesn't have the equipment so I'm looking for alternatives. Could I just put a drop of the BALF onto a charged slide and air dry it? Or treat the BALF like a blood smear? Alternatively, could I fix the cells in...ethanol maybe and then cytospin them the next day when I get back to my lab? Any ideas will be much appreciated. Jerry Ricks Research Scientist University of Washington Department of Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From histotech <@t> imagesbyhopper.com Thu Jun 2 19:49:14 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Jun 2 19:49:31 2011 Subject: [Histonet] RE: printed requisitions In-Reply-To: <25A4DE08332B19499904459F00AAACB71987E03D7E@EVS1.archildrens.org> References: <3AD061FE740D464FAC7BF6B5CFB75707013287@SMCMAIL01.somerset-healthcare.com> <25A4DE08332B19499904459F00AAACB71987E03D7E@EVS1.archildrens.org> Message-ID: <92F4768F-F009-41BB-A10C-02B799A4F400@imagesbyhopper.com> We currently use paper reqs, but coming soon, we will be going paperless. I am not sure I am going to like this... ;o). Surgical staff will enter the "req" in the computer, we receive the order & the specimen and enter into our AP system. There are going to be growing pains!!! Sent from my iPhone On Jun 2, 2011, at 8:18 AM, "Horn, Hazel V" wrote: > We still use paper reqs. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Autopsy/Histology/Transcription > Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni > Sent: Wednesday, June 01, 2011 3:13 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] printed requisitions > > > Does everyone still receive printed requisitions with your surgical specimens? How many of your OR's have a printer in each room? Our OR has a shared printer for all of the procedure rooms, but would like to have a printer for each OR suite. Our IT department is saying there would be problems and has suggested that we go paperless. I see many problems arising from this. If anyone is doing this successfully, I'd be very interested in hearing how you accomplished it. > > > Toni > > Regards, > Toni Rathborne > Pathology Supervisor > Somerset Medical Center > 110 Rehill Ave. > Somerville, NJ 08876 > > > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mshaeffer <@t> cox.net Thu Jun 2 22:33:45 2011 From: mshaeffer <@t> cox.net (Marc Shaeffer) Date: Thu Jun 2 22:46:45 2011 Subject: [Histonet] Working Ventana Probe Protocols Message-ID: <20110602233345.OBB79.737102.imail@fed1rmwml44> > Working Ventana Probe Protocols! > > Heres a good starting point when using the Kappa/Lambda/EBER Ventana Probes > on the Ultra: > Kappa Probe > > -Depar 16 min > - Enzyme Protease 2 for 4 min, > -ISH (Kappa) Probe 4min > -Denature @ 85 degrees for 12min > -Hybe 1 hour > - 3 stringency washes for 8 minutes each > -Blue detection for 20 minutes > -counterstain 4 min > Lambda and EBER Probes > > Same as Kappa but use the ISH (Lambda and or EBER) Probe 4 minutes. Everything else is > the same. > I also learned that there is no need to dilute out Ventana's probe as they > are predilutes and ready to use. The probe diluent that they sell is just > for using other probes. > Hopefully this helps everyone running these probes that need a starting point! I am not affiliated with ventana. Marc Shaeffer From dawudhill <@t> yahoo.com Thu Jun 2 23:27:38 2011 From: dawudhill <@t> yahoo.com (Dawud AlYasa) Date: Thu Jun 2 23:27:42 2011 Subject: [Histonet] Bone Marrow H&E Protocols Message-ID: <726474.45849.qm@web34506.mail.mud.yahoo.com> What's up Tena!? Long time. The bone marrows will be fixed in B-5. Dawud From j.brinker <@t> att.net Fri Jun 3 04:57:55 2011 From: j.brinker <@t> att.net (Jean Brinker) Date: Fri Jun 3 04:57:59 2011 Subject: [Histonet] nuclear haziness In-Reply-To: <687095.54911.qm@web65713.mail.ac4.yahoo.com> References: <207250.74842.qm@web180314.mail.gq1.yahoo.com> <687095.54911.qm@web65713.mail.ac4.yahoo.com> Message-ID: <50710.84775.qm@web180304.mail.gq1.yahoo.com> Thanks for your response. I had that thought as well, and the techs have adjusted their technique to make sure excess water is drained from slides before they are placed in the oven. They check them again before staining. ________________________________ From: Rene J Buesa To: Jean Brinker ; "histonet@lists.utsouthwestern.edu" Sent: Thu, June 2, 2011 9:50:08 AM Subject: Re: [Histonet] nuclear haziness Make sure that ALL your slides are properly dried in the oven and that NONE has any remnant of water underneath before going into the oven to dry. Ren? J. From: Jean Brinker To: histonet@lists.utsouthwestern.edu Sent: Thursday, June 2, 2011 6:03 AM Subject: [Histonet] nuclear haziness We are having an issue with nuclear haziness on prostate biopsies in our small urology laboratory.? It occurs randomly,?appearing on some levels and not others within the same block. Another scenario is that we sometimes see it occur only in one small area of a core and the remaining tissue in the core looks beautiful. It can occur throughout a case (12 blocks) or not at all. We have checked for obvious problems, i.e. reagent contamination on the processor and stainer, ruled out?temperature issues, microtome problems, etc. to no avail. I would appreciate any input/suggestions that would help to alleviate this problem. Thank you, Jean Brinker St. Louis Urological Surgeons St. Peters, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Fri Jun 3 05:09:14 2011 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Jun 3 05:13:33 2011 Subject: [Histonet] pricing for EM on kidney Message-ID: I've been asked to get pricing for Electron Microscopy on kidneys from labs that accept out reach samples. we are interested in your fee for EM only and not a complete kidney work up. In addition to pricing for kidney samples provided in gluteraldehyde, I'll also need to know if your pricing is different if you are asked to work from a paraffin block. thank you, Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 ph (843) 792-6353 fax (843) 792-8974 ________________________________________ From James.Reilly <@t> glasgow.ac.uk Fri Jun 3 06:42:32 2011 From: James.Reilly <@t> glasgow.ac.uk (Jim Reilly) Date: Fri Jun 3 06:42:37 2011 Subject: [Histonet] Counter top immunos Message-ID: Hello Kimberly We do all our immunos manually and the nearest thing to a kit we use is the ImmPRESS Peroxidase Polymer Detection System or VECTASTAIN ABC System with ImmPACT DAB Peroxidase Substrate all from Vector Laboratories. The majority of our Ab's are used on human tissue and the first choice for primary Ab's for us is mouse anti-human raised in horse. Cheers Jim Reilly Senior Histology Technician Institute Of Infection, Immunity, Inflammation College Of Medical, Veterinary and Life Sciences University Of Glasgow 120 University Place Glasgow G12 8TA Tel: +44 141 330 7573 From Rcartun <@t> harthosp.org Fri Jun 3 10:55:21 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jun 3 10:55:27 2011 Subject: [Histonet] Submission of tonsils to pathology Message-ID: <4DE8CBA8.7400.0077.1@harthosp.org> What is your policy on the submission of tonsils to pathology for examination (gross or microscopic)? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From kstoll <@t> mcw.edu Fri Jun 3 11:03:27 2011 From: kstoll <@t> mcw.edu (Stoll, Kathryn) Date: Fri Jun 3 11:03:32 2011 Subject: [Histonet] Processing Mouse Pancreas Message-ID: <110E7925E2B91945A9B79EDFD0DC2B34E2A188859B@MCWMBX2.mcwcorp.net> Happy Friday Everyone, Does anyone have a protocol for processing mouse pancreas? We have a VIP6. I am not familiar with the processing any animal tissue and do not know if it is similar to human. Thanks in advance. Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kstoll@mcw.edu From rjbuesa <@t> yahoo.com Fri Jun 3 11:10:35 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 3 11:10:38 2011 Subject: [Histonet] Submission of tonsils to pathology In-Reply-To: <4DE8CBA8.7400.0077.1@harthosp.org> Message-ID: <231345.32485.qm@web65716.mail.ac4.yahoo.com> Labelled "gross only". Ren? J. --- On Fri, 6/3/11, Richard Cartun wrote: From: Richard Cartun Subject: [Histonet] Submission of tonsils to pathology To: "Histonet" Date: Friday, June 3, 2011, 11:55 AM What is your policy on the submission of tonsils to pathology for examination (gross or microscopic)?? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT? 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jun 3 11:12:18 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 3 11:12:22 2011 Subject: [Histonet] Processing Mouse Pancreas In-Reply-To: <110E7925E2B91945A9B79EDFD0DC2B34E2A188859B@MCWMBX2.mcwcorp.net> Message-ID: <722585.84511.qm@web65712.mail.ac4.yahoo.com> Develop a good general protocol for animal tissue. You cannot have "dedicated" protocols for every type of tissue. Ren? J. --- On Fri, 6/3/11, Stoll, Kathryn wrote: From: Stoll, Kathryn Subject: [Histonet] Processing Mouse Pancreas To: "histonet@lists.utsouthwestern.edu" Date: Friday, June 3, 2011, 12:03 PM Happy Friday Everyone, Does anyone have a protocol for processing mouse pancreas?? We have a VIP6. I am not familiar with the processing any animal tissue and do not know if it is similar? to human. Thanks in advance. Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kstoll@mcw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Fri Jun 3 11:13:44 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Jun 3 11:13:50 2011 Subject: [Histonet] Submission of tonsils to pathology In-Reply-To: <231345.32485.qm@web65716.mail.ac4.yahoo.com> References: <4DE8CBA8.7400.0077.1@harthosp.org> <231345.32485.qm@web65716.mail.ac4.yahoo.com> Message-ID: <25A4DE08332B19499904459F00AAACB71987E03D8B@EVS1.archildrens.org> Generally all are gross only. There are some exceptions depending on patient history or if one is significantly larger than the other the pathologists have the discretion to do a microscopic. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, June 03, 2011 11:11 AM To: Histonet; Richard Cartun Subject: Re: [Histonet] Submission of tonsils to pathology Labelled "gross only". Ren? J. --- On Fri, 6/3/11, Richard Cartun wrote: From: Richard Cartun Subject: [Histonet] Submission of tonsils to pathology To: "Histonet" Date: Friday, June 3, 2011, 11:55 AM What is your policy on the submission of tonsils to pathology for examination (gross or microscopic)?? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT? 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Rcartun <@t> harthosp.org Fri Jun 3 11:40:47 2011 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jun 3 11:40:55 2011 Subject: [Histonet] Submission of tonsils to Pathology - #2 References: <4DE8D0F1020000770002764F@gwmail1.harthosp.org> <4DE8D3FE0200007700027658@gwmail1.harthosp.org> <4DE8D64F020000770002765B@gwmail1.harthosp.org> Message-ID: <4DE8D64E.7400.0077.0@harthosp.org> I apologize; I should have stated my question differently. Our Children's Hospital wants to stop sending tonsils to Pathology unless the surgeon requests pathological examination. An article published in Otolaryngology-Head and Neck Surgery (Nelson et al.) concluded that pathologic examination of tonsils is not a cost-effective use of limited health care resources. "Zero cases of unsuspected pathology were identified on planned gross specimen evaluation (n=4186) and positive pathologic findings on microscopic analysis (n=1066) were only identified in post-transplant patients." Have any of you exempted tonsils from submission to Pathology? If not, maybe we should. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From LINDA.MARGRAF <@t> childrens.com Fri Jun 3 13:08:23 2011 From: LINDA.MARGRAF <@t> childrens.com (Linda Margraf) Date: Fri Jun 3 13:08:27 2011 Subject: [Histonet] Submission of tonsils to Pathology - #2 In-Reply-To: <4DE8D64E.7400.0077.0@harthosp.org> References: <4DE8D0F1020000770002764F@gwmail1.harthosp.org> <4DE8D3FE0200007700027658@gwmail1.harthosp.org> <4DE8D64F020000770002765B@gwmail1.harthosp.org> <4DE8D64E.7400.0077.0@harthosp.org> Message-ID: <683621D7852C2F488898D0AC7F164A98882DC3E9@CMCPBEXMAIL02.Childrens.med> Richard; Our children's Hospital (Children's Medical Center Dallas where I am the lab's Medical Director) made tonsils exempt from required pathologic examination 8 or so years ago following approval of the medical and surgical staff. We now only receive tonsils the surgeons are concerned about (transplant patients, asymmetric lesions etc.). Our surgical case accession numbers dropped by about 800 as a result of this but we do feel we are doing what's most cost effective yet beneficial to the patient. We get one or two cases a week now. We have about 15 other items on our list of specimens that are exempt from pathologic review. It is important to have the pathologists work with the clinicians to generate the list of tissue specimens that don't need to come to pathology. Linda M Histonet administrator -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, June 03, 2011 11:41 AM To: Histonet Subject: [Histonet] Submission of tonsils to Pathology - #2 I apologize; I should have stated my question differently. Our Children's Hospital wants to stop sending tonsils to Pathology unless the surgeon requests pathological examination. An article published in Otolaryngology-Head and Neck Surgery (Nelson et al.) concluded that pathologic examination of tonsils is not a cost-effective use of limited health care resources. "Zero cases of unsuspected pathology were identified on planned gross specimen evaluation (n=4186) and positive pathologic findings on microscopic analysis (n=1066) were only identified in post-transplant patients." Have any of you exempted tonsils from submission to Pathology? If not, maybe we should. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From rsrichmond <@t> gmail.com Fri Jun 3 13:16:09 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Jun 3 13:16:14 2011 Subject: [Histonet] Re: Submission of tonsils to Pathology Message-ID: I have no idea how many tonsils I've looked at since finishing residency in 1972, but I haven't seen a clinically significant diagnosis made on a tonsillectomy specimen since then. In residency at Johns Hopkins around 1970, I did see two significant diagnoses made. I made the diagnosis of tuberculosis on a two year old child myself - we were actually able to stain the organisms. I don't know the follow-up. - Somebody else made a diagnosis of lymphoma on a child's tonsil, but I suspect the diagnosis was wrong. The question for the pathologist is entirely one of revenue. But to enlarge the question of revenue - children in great numbers are still being subjected to this painful and hazardous procedure - I still see tonsillectomy specimens the same day and same last name (from siblings, I mean) very frequently. If we really want to save the health care system money, we need to greatly reduce the number of tonsillectomies done. It's basically an ethnosurgical procedure, like circumcision and ear piercing. Bob Richmond Samurai Pathologist Knoxville TN From cconlisk <@t> kcskincenter.com Fri Jun 3 13:17:37 2011 From: cconlisk <@t> kcskincenter.com (Christopher Conlisk) Date: Fri Jun 3 13:17:41 2011 Subject: [Histonet] Slide Bright Question Message-ID: Hello Everyone, I have worked in labs that use Xylene my entire career and I just started at a Lab That only uses a Xylene Substitute "Slide-Bright". I am having problems with the H&E. After staining and coverslipping (The slides look fine innitially), Then about 5-10 minutes after coverslipping the Eosin starts bleeding out all around the tissue. I have asked several of my Histotech Friends that are old timers and they say that Xylene Substitutes are awful at deparrifinization and awful at clearing. They told me that the alcohol isnt getting thoroughly cleared in the "Slide Brite" and then it is eventually leeching out after coverslipping??? Is this true and does anyone have any guidance for this issue? We also run MOHS slides on the same stainer and I keep all the reagents clean as a whistle. I really hate Xylene Substitute's.... Thanks C.S. Conlisk HT(ASCP), PBT(ASCP) Kansas City Skin and Cancer Center From sgoebel <@t> mirnarx.com Fri Jun 3 13:28:37 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Fri Jun 3 13:28:41 2011 Subject: [Histonet] Slide Bright Question In-Reply-To: References: Message-ID: Ahhh!!! I had this same problem when I came to this lab too!! Plus if you put that xylene substitute crap (mine was clear rite) it screws up cell blocks that have been embedded into agar!! 5 experiments had to be repeated because of this stuff!! The eosin bleed is because you got water on the slides after the eosin. With xylene you can see the water pool at the bottom, but with these substitutes it just kind of becomes part of the solution. I have finally gone back to xylene! We had to buy hoods, but it's the cost of better histology =) Fixing the eosin bleed...uncoverslip, run through xylene (or let sit in the substitute for 15 or 20 minutes, then alcohols, then in running water for 10 minutes or so. Then change all your alcohols to fresh solutions (I would change the substitutes too), then from water to alcohol, eosin, etc. etc. and they should be fine. Good luck!! I wanted to pull my hair out a couple months ago because of that stuff...run away from it!!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christopher Conlisk Sent: Friday, June 03, 2011 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Bright Question Hello Everyone, I have worked in labs that use Xylene my entire career and I just started at a Lab That only uses a Xylene Substitute "Slide-Bright". I am having problems with the H&E. After staining and coverslipping (The slides look fine innitially), Then about 5-10 minutes after coverslipping the Eosin starts bleeding out all around the tissue. I have asked several of my Histotech Friends that are old timers and they say that Xylene Substitutes are awful at deparrifinization and awful at clearing. They told me that the alcohol isnt getting thoroughly cleared in the "Slide Brite" and then it is eventually leeching out after coverslipping??? Is this true and does anyone have any guidance for this issue? We also run MOHS slides on the same stainer and I keep all the reagents clean as a whistle. I really hate Xylene Substitute's.... Thanks C.S. Conlisk HT(ASCP), PBT(ASCP) Kansas City Skin and Cancer Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSiena <@t> statlab.com Fri Jun 3 13:31:29 2011 From: DSiena <@t> statlab.com (Debra Siena) Date: Fri Jun 3 13:31:33 2011 Subject: [Histonet] Slide Bright Question In-Reply-To: References: Message-ID: Hi Christopher, There are differences between how Xylene substitutes works and Xylene. First of all, Xylene subs can be a bit slower to deparaffinize, so you may need to increase the time or # or stations when deparaffinizing. Also, Xylene subs don't tolerate water so your last alcohol must be 100% or else you will see the eosin bleed out of the sections. The other thing about Xylene substitutes is that the mounting media must be compatible with the substitute. If I can help in any other way, please let me know. thanks Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products Direct: 972-436-1010? x229 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christopher Conlisk Sent: Friday, June 03, 2011 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Bright Question Hello Everyone, I have worked in labs that use Xylene my entire career and I just started at a Lab That only uses a Xylene Substitute "Slide-Bright". I am having problems with the H&E. After staining and coverslipping (The slides look fine innitially), Then about 5-10 minutes after coverslipping the Eosin starts bleeding out all around the tissue. I have asked several of my Histotech Friends that are old timers and they say that Xylene Substitutes are awful at deparrifinization and awful at clearing. They told me that the alcohol isnt getting thoroughly cleared in the "Slide Brite" and then it is eventually leeching out after coverslipping??? Is this true and does anyone have any guidance for this issue? We also run MOHS slides on the same stainer and I keep all the reagents clean as a whistle. I really hate Xylene Substitute's.... Thanks C.S. Conlisk HT(ASCP), PBT(ASCP) Kansas City Skin and Cancer Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From campbellj <@t> muhlbauerlab.com Fri Jun 3 13:37:20 2011 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Fri Jun 3 13:37:25 2011 Subject: [Histonet] Slide Bright Question In-Reply-To: References: Message-ID: We use ProPar and have found a way to survive the issue. Our stainer racks cause a lot of solution carryover so we rotate the alcohols after eosin after every third run. Once there is water in your final clearing containers they must be changed. Jen Campbell, HT(ASCP) On Fri, Jun 3, 2011 at 2:28 PM, wrote: > Ahhh!!! I had this same problem when I came to this lab too!! Plus if > you put that xylene substitute crap (mine was clear rite) it screws up > cell blocks that have been embedded into agar!! 5 experiments had to be > repeated because of this stuff!! The eosin bleed is because you got > water on the slides after the eosin. With xylene you can see the water > pool at the bottom, but with these substitutes it just kind of becomes > part of the solution. I have finally gone back to xylene! We had to > buy hoods, but it's the cost of better histology =) > > Fixing the eosin bleed...uncoverslip, run through xylene (or let sit in > the substitute for 15 or 20 minutes, then alcohols, then in running > water for 10 minutes or so. Then change all your alcohols to fresh > solutions (I would change the substitutes too), then from water to > alcohol, eosin, etc. etc. and they should be fine. > > Good luck!! I wanted to pull my hair out a couple months ago because of > that stuff...run away from it!!! > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Christopher Conlisk > Sent: Friday, June 03, 2011 1:18 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide Bright Question > > Hello Everyone, > I have worked in labs that use Xylene my entire career and I just > started at a Lab That only uses a Xylene Substitute "Slide-Bright". I am > having problems with the H&E. After staining and coverslipping (The > slides > look fine innitially), Then about 5-10 minutes after coverslipping the > Eosin > starts bleeding out all around the tissue. I have asked several of my > Histotech Friends that are old timers and they say that Xylene > Substitutes > are awful at deparrifinization and awful at clearing. They told me that > the > alcohol isnt getting thoroughly cleared in the "Slide Brite" and then it > is > eventually leeching out after coverslipping??? Is this true and does > anyone > have any guidance for this issue? We also run MOHS slides on the same > stainer and I keep all the reagents clean as a whistle. I really hate > Xylene > Substitute's.... > > Thanks > > C.S. Conlisk HT(ASCP), PBT(ASCP) > Kansas City Skin and Cancer Center > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 From DKBoyd <@t> chs.net Fri Jun 3 13:49:53 2011 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Fri Jun 3 13:50:02 2011 Subject: [Histonet] Slide Bright Question In-Reply-To: Message-ID: Christopher I use Americlear and have not had a staining problem. Your last alcohols must be absolute and as stated previously your mounting media must be compatible. I do not, however, use Americlear on the processor as we did have processing problems there. We use ProPar from Anatech on the processor. With the expense and carcinogen factor of Xylene, this set up works great for us. As with any clearing agent, water contamination , produces leching eosin as one of many problems. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net Christopher Conlisk Sent by: histonet-bounces@lists.utsouthwestern.edu 06/03/2011 02:17 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Slide Bright Question Hello Everyone, I have worked in labs that use Xylene my entire career and I just started at a Lab That only uses a Xylene Substitute "Slide-Bright". I am having problems with the H&E. After staining and coverslipping (The slides look fine innitially), Then about 5-10 minutes after coverslipping the Eosin starts bleeding out all around the tissue. I have asked several of my Histotech Friends that are old timers and they say that Xylene Substitutes are awful at deparrifinization and awful at clearing. They told me that the alcohol isnt getting thoroughly cleared in the "Slide Brite" and then it is eventually leeching out after coverslipping??? Is this true and does anyone have any guidance for this issue? We also run MOHS slides on the same stainer and I keep all the reagents clean as a whistle. I really hate Xylene Substitute's.... Thanks C.S. Conlisk HT(ASCP), PBT(ASCP) Kansas City Skin and Cancer Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From madary <@t> verizon.net Fri Jun 3 14:15:21 2011 From: madary <@t> verizon.net (madary@verizon.net) Date: Fri Jun 3 14:15:31 2011 Subject: [Histonet] hand staining, bench top manual no automation Message-ID: <1097311366.1062458.1307128521181.JavaMail.root@vznit170070> When I was in the field of histology and performing IHC manually, I fo usage, eas reuse). Slide a waiting for the next job,any Nick(Rocky) Madary, HT/HTL(ASCP)QIHC Jun 3, 2011 12:01:25 P Send Histonet histonet@lists.utsouthwestern.edu To http://lists.uts or, via email, send a message histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lis When replying, please edit your Subject line s specific than "Re: Contents of Histonet digest..." < 1. RE: Counter top immunos (Smith, Allen) 2. l 3. RE: [IHCRG] lam Chlipala) 4. RE: Counter top immu 5. Alternatives to Cytospin? (JR R) 6. RE: Counter top 7. RE: Alternatives to Cytospin? (Tony Henwood) 8. 9. Working V 10. Re: Bone Marrow H&E Proto 11. Re: nuclear haziness (Jean Brinker) 12. prici 13. Counter top immunos (Ji 14. Submission of tonsils to pathology (Richard Cartun) 15. 16. Re: Submission of tonsil 17. Re: Processing Mouse Pancreas (Rene J 18. RE: Submission of tonsils to pathology (Horn, Hazel V) 19. ------ ---------------------------------------------------------------- Mes Date: Thu, 2 Jun 2011 18:05:30 +0000 From: "Smith, Allen" Subject: [Histonet] RE: Counter top immunos To: Cc: "histonet@lists.utsout Message-ID: I like the Vector ABC the Nova Red chromogen. Mouse better results than rabbit or goat prim -----Original Message----- From: histonet-bounces@lists.ut [mailto:histonet-bounces@lists.utsouthwestern.edu] On Beha Marshall, Kimberly K Sent: Thursday, June 02, 2011 9:57 AM To: Subject: [Histonet] Counter top immuno Hello Histo folks Hope everyone is having a great week....I info on immuno kits for staining just by hand, l started in this field. We are a small lab in Alask immunos are sent out of state, We are looking to do som to save money, but don't really need a stainer. S kits that are better than others? Is there anyone that d this way that would give some much needed advise??? A Kimberly ____________ Histonet mailing list Histonet@li http://lists.utsouthwestern.edu/mailman/listinfo/ ------------------------------ Message: Date: Thu, 2 Jun 2011 13:05:28 -0500 From: "Perry, Margaret" Subject: [Histonet] laminin and collagen4 in cat To: "ihcrg@googlegroups.com" , "histo Mes Content-Type: text/plain; charset="us-ascii" We want to use some spindle cell tumors. sheath tumors reportedly show lam membranes vs. Fibrosarcoma which does not. We will work in both cat and dog. Is there anyone who ha success with these? Do you have any recommendations for these antibod Margaret Perry HT(ASCP) Dept of Veterina Box 2175 South Dakota State Univer Brookings SD 57007 605-688-5638 - Message: 3 Date: Thu, 2 Jun 2011 12 From: "Liz Chlipala" Subject: [Hist dog To: "Perry, Marga , Message-ID: Content-Type: text/plain; charset="us Margaret We have used the Collagen IV listed feline, for laminin we have not used the antibod feline but the spec sheet states it works on m Good Luck Liz Col SouternBio 0340-01 UNLB Goat Laminin abcam ab11575 Rabbit Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager PO Box 18592 Boulder, Colorado 80308< office (303) 682-3949 fax (303) 682-9060 www.premierl Ship to Address: 1567 Skyway Drive, Un Longmont, Colorado 80504 -----Original Message----- F Behalf Of Sent: Thursday, June 02, 2011 12:05 PM To: ihcrg@goo Subject: [IHCRG] lamini We want to use laminin and Co some spindle cell tumors. Specifical sheath tumors reportedly show laminin and vs. Fibrosarcoma which does not. We need both cat and dog. Is there anyone who has you have any recommendations for these antibo Margaret Perry HT(ASCP) Dept of Veter Box 2175 South Dakota State Uni Brookings SD 57007 605-688-5638 -- You received this message because you are subscribed to the Google Grou committee within To post to this group, sen To unsubscribe from this group, send e ihcrg+unsubscribe@googlegroups.com For more options, visit th http://groups.google.com/group/ihcrg?hl=en To conta histo@nsh.org or cal ------------------------------ Messag Date: Thu, 2 Jun 2011 13:50:43 -0500 From: hymclab Subject: [Histonet] RE: Counter top immunos T Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/pla We do ours manually also (about 20 differen a combo of BioGenex and Dako. I like both set ups, bu more expensive to perform than BioGenex. I like that you can buy the in dividual components from BioGenex instead of a whole ket from DAKO.< Dawn -----Original Message----- From: histonet-bounces@lis [mailto:histonet-bounces@lists.utsouthwestern.edu] On Smith, Allen Sent: Thursday, June 02, 2011 1:06 PM To: Mar Cc: histonet@lists.utsouthwestern.edu Subject: [His I like the Vector ABC horseradish per with the Nova Red chromogen. Mouse primary antibod give me better results than rabbit or goat primaries. -- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Sent: Thursday, June 02, 2011 9:57 AM To: histonet@lists.u Subject: [Histonet] Counter top immunos Hello H Hope everyone is having a great week....I am writing to g info on immuno kits for staining just by hand, like back when I fir of our immun of them here and try to save money, but don't really need a stainer. So are there any kits t this way that Again thanks in advance for a Kimberly ________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu ht ________ Histonet mailing list Histone http://lists.utsouthwestern.edu/mailman/listi CONFIDENTIALITY NOTICE: This e-mail communication and a attachments may contain confidential and privileged information for the not the intended received this communicati disclosure, dissemination, distribution or contents is prohibited. If you have received this com in error, please notify the sender at the electronic mail addres noted above and destroy all copies of this communication and any attachme ----------------------- Message: 5 Date: Thu, 2 Jun 2011 16:54:29 -0700 From: Subject: [Histonet] Alternatives to Cyto To: Message-ID: Content-Type: text/plain; charset=" I will be collecting bronchoalveolar lavage fluid (B mice and want to do do a diff-quick stain on the fluid to charact erize the leukocyte population. My colleagues usually use a cytospin slide but I'll be at a lab that doesn't have t looking for alternatives. Could I just put a dro dry it? Or treat the BALF like a Alternatively, could I fix the cells in...ethanol mayb cytospin them the next day when I get back to my lab? Any Jerry Ricks Research ScientistUniversity of Washington Department of Pathology -- Message: 6 Date: Thu, 2 Jun 2011 17: From: JR R Subject: [Histonet] To: M Content-Type: t For mouse and rabbit primary a ImmPRESS kits. They save time. For HRP substrat too dull to me. I have had good results with AEC and also Vector ImmPACT DAB. Jerry Ricks Research Scientist Unive Department of Pathology -----Original Message From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-b ounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: To: histonet@lists.utsouthwestern.eduSubject: [Histonet] Counter top immunos Hello Histo folks H info on imm first started in this of our immunos are sent out of of them here and try to save money, but that are better than this way that would give some mu Again thanks in advance for all the imput... Kimberly ------------------------------ Message: 7 Da From: Tony Henwood Subject: RE: [Histonet] Alternatives to Cytospin? To: "'JR R'" , "histonet@lists.utsouthwestern.edu" Message-ID: <6D6BD1DE8A5571489398B392A38 A7157188590A3@xmdb02.nch.kids> Content-Type: text/plain; charset="u Jerry, Firstly, I would not recommend fixing in etha shrink and Romanowsky staining will be compromised (th stain too darkly since they are smaller). I would use a cent a blood film or if you do no a drop of the BALF on the slide and ei suggested (if cell count is low) or treat it like blood looks quite opaque - lots of cells/ & other junk). Regar Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fa the children's hospital at westmead Cnr Hawkesbury Locked Bag 4001, Westmead NSW 2145, -----Original Message----- From: histonet-bounces@lis [mailto:histonet-bounces@lists.utsouthwestern.edu] On R Sent: Friday, 3 June 2011 9:54 AM To: histonet@lists. Subject: [Histonet] Alternatives to Cytospin? I will be collecting bronchoalveolar lavage fluid (BALF) from mice and the leukocyte My colleagues usually use a cytospin to concentrate cel slide but I'll be at a lab that doesn't have the equipment so I'm looking for alternatives. Could I just put a drop of the BALF onto dry it? Or treat the BALF like a blood smear? Alternatively, could I fix the cells in...ethanol maybe and then cytospin Any ideas will be much Jerry Ricks Research Scientist University of Was Department of Pathology _____________________________ Histonet mailing list Histonet@lists.utsouthwester http://lists.utsouthwestern.edu/mailman/listinfo/histonet * ******************************************************************* ******** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are ad delete it and notify Views expressed in this message and any attachments are the individual sender, and are not necessarily the views of The C hildren's Hospital at Westmead This note also confirms that this ema scanned and although no computer viruses were det Childrens Hospital at Westmead accepts no liability for any cons equential damage resulting from email containing computer viruses. ***** ******************************************************************* ******** ------------------------------ Message: 8 Date: From: "histotech@imagesbyhopper.com" Subject: Re: [Histonet] RE: printed requisit To: "Horn, Hazel V" Cc: "histonet@lists Message-ID: <92F4768F-F009-41BB-A10C-02B799A4F400@imagesbyhopper.com> Content- We currently use paper reqs, bu paperless. I am not sure I am going t staff will enter the "req" in the computer, we the specimen and enter into our AP system. Sent from my iPhone On Jun 2, 2011, at 8:18 AM, "Horn, Hazel V" wrote > We still use paper reqs. > > Hazel Horn > > Supervisor of Autopsy/Histology/Transcrip > Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > > > visit us on the web at: www.archildrens.o > > > -----Original Message----- > From: histo [mailto:histonet-bounces@lists.utsouth Rathborne, Toni > Sent: Wednesday, June 01, > To: histonet@lists.utsouthwestern.edu > Subject > > > Does everyone s surgical specimens? How many of room? Our OR has a shared printer for all but would like to have a printer for each OR suite department is saying there would be problems and has suggested tha t we go paperless. I see many problems arising from this. If anyone is doin you accomplished > > > Toni > > Regards, > Toni > Pathology Supervisor > Somerset Medical Center & > Somerville, NJ 08876 > > > > > CONFIDENTIALITY NOTICE > This message and any inclu Medical Center > and are intended o contained in this > message is confidential, > proprietary protection and/or > exem forwarding, > strictly> prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to > www.somersetmedicalcent > event listings, health info > _______________________________________________> Histonet mailing list > Histonet@lists.utsouthwestern.edu & > & ******************************************************************* **** ******************************************************************* ******** ******************************************************************* ******** ******************************************************************* ******** ******************************************************************* ******** ******************************************************************* ******** ******************************************************************* ******** ******************************************************************* ******** > The information contained in thi confidential > and protected from dis not the > intended recipie delivering this > messag any > diss strictly &g error, please not > us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________ > Histonet mailing list > Histonet@lis > http://lists.utsouthwestern.edu/mailman/listi > ------------------------------ Date: Thu, 2 Jun 2011 20:33:45 -0700 From: Marc Shaeffer < Subject: [Histonet] Working Ventana Probe ProtocolsTo: histonet@lists.utsouthwestern.edu Message-ID: <20110602233345.O Content-Type: text/plain; charset=ut > Working Ventana Probe Protocols! > > Heres a Ventana Probes > > Kappa Probe > > -Depar 16 min & > -ISH (Kappa) Probe 4min > > -Hybe 1 hour > - 3 stringenc > -Blue detection for 20 minutes > > Lambda and EBER Probes > > Sam minutes. Everything > the same. > I also learned that there is no need probe as they > are predilutes and ready to sell is just > for using other probe > Hopefully this helps everyone running these probes that nee starting point! I am not affiliated with ventana. Marc S ------------------------------ Message: 10 Date: Thu, 2 Jun 2011 21:27:38 -0700 (PDT) From: Dawud AlYasa Subject: Re: [Histonet] Bone Marrow H&E Protocols To Message-ID: <726474.45849.qm@web3 Content-Type: text/plain; charset=iso-8859 What's up Tena! Long time. The bone marrows will be fixe Dawud ------------------------------ Message: 11 Date: Fri, 3 Jun 2011 02:57:55 -0700 (PDT) From: Jean Brinker Subject: Re: [Histonet] nuclear haziness To: Rene J Bue "histonet@lists.utsouthwestern.edu" Message-ID: <50710.84775.qm@web180304.mail Content-Type: text/plain; charset=iso-8859-1 Thanks for your response. I had that thought as well, and the techs have < drained from slide they are placed in the oven. They check them again before stai ning. ________________________________ From: Ren To: Jean Brinker ; "his Subject: Re: [Histonet] nuclear hazin Make sure that ALL your slides are properly dried in the ove that NONE has any remnant of water underneath before going into t dry. Ren? J. From: Jean Brinker To: histonet@lists.utsouthwestern.edu Sent: Thursday, June 2, 2011 Subject: [Histonet] nuclear haziness We are having an is in our small urology lab and not ot within the same block. Another scenario is that we sometimes s it occur only in one small area of a core and the remaining tissue i looks beautiful. It can occur throughout a case (12 blocks) all. We have checked for obvious problems, i.e. reagent conta processor and stainer, ruled out temperature issue to no avail. I would appreciate any input/s alleviate this problem. Thank you Jean Brinker St. Louis Urological Surgeons St. Peters, MO _______________________________________________ Histonet mailing listHistonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mail ------------------------------ Message: Date: Fri, 3 Jun 2011 06:09:14 -0400 From: "Della Speranza, Vinni Subject: [Histonet] pricing for EM on kidney To: Message-ID: Content-Type: text/plain; charset="us-ascii" I've been from labs that acce your fee for EM only and not a c In addition to pricing for kidney samples pr gluteraldehyde, I'll also need to know if your pricing is differe nt if you are asked to work from a paraffin block. thank you, Vinnie Della Speranza, MS, HTL(ASCP) Manager for Anatomic Pathology Ser Medical University of South Carolina 165 Ashley Avenue Suite 30 Charleston, SC 29425 ph (843) 792-6353 fax (843) 792-8974 ___ ---------- Message: 13 Date: Fri, 3 Jun 2011 12:42:32 + From: "Jim Reilly" Subject: [Histon To: Messa Content-Type: text/plain; charset="us-ascii" Hello We do all our immunos manually and the nearest thing to a use is the ImmPRESS Peroxidase Polymer Detection System or VECTAS ABC System with ImmPACT DAB Peroxidase Substrate all from Vector Laboratories. The majority of our Ab's are used on human tissue and the first choice for primary Ab's for us is mouse anti-human raised in hors Cheers Jim Reilly Senior Histology Technician Instit Medical, Veterinary Place Glasgow Tel: +44 141 330 7573 ----------------------- Message: 14 Date: Fri, 03 Jun 2011 11:55:21 -0400 From Subject: [Histonet] Submission To: "Histonet" Message-ID: <4DE8CBA8.7400.0077.1@harthosp.org> Content-Typ What is your policy on the submiss examination (gross or microscopic)? Thank y Richard Richard W. Cartun, MS, PhD Director, Histology Director, Biospecimen Collection Programs Assi Hartford Hospital 80 Seymour Stree Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax < ------------------------------ Message: 15 Dat From: "Stoll, Kathryn" Subject: [Histonet] Processing Mouse Pancreas To: "histonet@lists.u Message-ID: <110E7925E2B91945A9B79EDFD0DC2B34E2A188859B@MCWMBX2.mcwcorp.net> Happy Friday Everyone Does anyone have a protocol for processing mouse pancreas? We have a V I am not familiar with the processing any animal tissue and do not Thanks in advance. Kathryn St Depatment of Pathology Medical College of Wisconsin Milwaukee WI 53226 414.805.1525 kstoll@mcw.ed ------------------------------ Message: 16 Date: From: Rene J Buesa Subject: Re: [Histonet] Submission of tonsils to pathology To: H Message-ID: <231345.32485.qm@web65716.mail.ac4.yahoo.com&g Content-Type: text/plain; charset=iso-8859-1 Labelled "gross Ren? J. --- On Fri, 6/3/11, Richard Cartun wrote: From: Richard Cartun < tonsils to pathology To: "Histonet" Date: Friday, June 3, 2011, 11:55 A What is your policy on the submission of tonsils to pathology examination (gross or microscopic)? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatom Hartford Hospital 80 Seymour Street Hartford, CT (860) 545-1596 Office (860) 545-2204 Fax _____ Histonet mailing list Hist http://lists.utsouthwestern.edu/mailman/li ------------------------------ Message: 1 Date: Fri, 3 Jun 2011 09:12:18 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Processing Mouse Pancreas To: , Message-ID: <722585.84511.qm@web65712. Content-Type: text/plain; charset=iso-8859-1 Develop a good general protocol for animal tissue. You cannot have "dedicated" protocols for every type of tissue. Ren? J. --- From: Sto Subject: [Histonet] Processing Mouse Pancre To: "histonet@lists.utsouthwestern.edu" Date: Friday, June 3, 2011, 12:03 PM Happy Friday Ever Does anyone have a protocol for processing mouse pancreas? W have a VIP6. I am not familiar with the processing any animal tissue a not know if it is similar to human. Thanks in advance. Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525< _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists ------------------ Message: 18 Date: Fri, 3 Jun 2011 11:13:44 -0500 Subject: RE: [Histonet] S To: "'Rene J Buesa'" , Histonet , Richard Cartun Message-ID: <25A4DE08332B19499904459F00AAACB719 Content-Type: text/plain; charset="i Generally all are gross only. There are some exceptions d on patient history or if one is significantly larger than the othe r the pathologists have the discretion to do a microscopic. Hazel H Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcr Arkansas Children's Hospital 1 Children's Way Slot 820 Litt phone 501.364.4240 fax 501.364.3155 visi -----Original Message--- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-boun Rene J Buesa Sent: Friday, Ju To: Histonet; Richard Cartun Subject: Re: [Histo Labelled "gross only". Re --- On Fri, 6/3/11, Richard Cartun From: Richard Cartun Subject: [ To: "Histonet" Date: Friday, June 3, 2011, 11:55 AM examinati Richard Richard W Director, Histology & Immunopathology Director, Assistant Director, Anatomic Pathology< 80 Seymour Street Hartford, CT 06102 (8 (860) 545-2204 Fax __________________ Histonet mailing list Histonet@lists.ut http://lists.utsouthwestern.edu/mailman/listinfo/histon _______________________________________________ Histonet mailing l Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu ***************************************** ******************************************************************* ******** ******************************************************************* ******** ******************************************************************* ******** ******************************************************************* ******** ******************************************************************* ******** ******************************************************************* ******** ******************************************************************* ******** **************************************************************** The inf confidential an not the i delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly < in error, please not us immediately by replying to the message and deleting it from your Thank you. ------------------------------ < Date: Fri, 03 Jun 2011 12:40:47 -0400 From: "Richard C Subject: [Histonet] Submission of tonsils To: "Histonet" Content-Type: text I apologize; I should have stated my ques Children's Hospital wants to stop sending tonsils to unless the surgeon requests pathological examination. An article published in Otolaryngology-Head and Neck Surgery (Nelson et al.) concluded cost-effective use of limi of unsuspected pathology were identi specimen evaluation (n=4186) and positive pathologi on microscopic analysis (n=1066) were only identified in post- transplant patients." Have any of you exempted tonsils from submissi not, maybe we should. Richard Richard W. Director, Histology & Immunopathology Director, B Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545- (860) 545-2204 Fax ------------------ _______________________________________________ Hist Histonet@lists.utsouthwestern.edu http://lists.utso End of Histonet Digest, Vol *************************************** From k84as <@t> yahoo.com Fri Jun 3 14:21:31 2011 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Fri Jun 3 14:21:34 2011 Subject: [Histonet] why no replay?? Message-ID: <481818.50221.qm@web112605.mail.gq1.yahoo.com> i have posted my quistion 2-3 times several days ago and recieve no negative or positive comment !!! why?? is my quistion not clear or what?? --- On Thu, 6/2/11, mohamed abd el razik wrote: From: mohamed abd el razik Subject: Alcian blue quistion To: histonet@lists.utsouthwestern.edu Date: Thursday, June 2, 2011, 10:53 AM hi every body i have a confusing quistion reagrding Alcian blue pH 1 & 2.5 stains. suppose you have 100 goblet cell react positive to pH 1 which mean all have sulfated mucin. and 50 cell from other section react positive to pH 2.5 which mean acidic type(sulfated or sialated). why these 50 cell only react to pH 2.5 althogh the other non reacted cells possess acidic sulfated type?? on other word i need to know for what type of mucins alcian B 2.5 react with? ? thanx all From cpyse <@t> x-celllab.com Fri Jun 3 14:25:06 2011 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Fri Jun 3 14:25:58 2011 Subject: [Histonet] validation sheet Message-ID: <002401cc2223$f25c0b60$d7142220$@com> Happy Friday Histonetters Does anyone have a validation log sheet they have used for the validation of a recycler. Someone spilled xylene on our copy then proceeded to discard it without coping the sheet first. I would appreciate it if anyone would share the log sheet they have used, it would save me some time. Thanks in advance. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com From madary <@t> verizon.net Fri Jun 3 14:25:54 2011 From: madary <@t> verizon.net (madary@verizon.net) Date: Fri Jun 3 14:26:08 2011 Subject: [Histonet] self employed vs contractor with a company Message-ID: <172859641.1062998.1307129154760.JavaMail.root@vznit170070> At 50 I find myself to be an unemployed histotech. The positive i that I can smell again! So I have established a relationship with a few c and wide.&nbs my cut and the hiri spent on expendables. M has gone the route of being a sel through contract agencies, but still is self purposes? Maybe I should ask, if there are lab mana who hire out for short bursts of time because someone is on maternity, FMLA etc, what prompts you to hire through a contract agency ins self employed or ju expectation of the normal be dental? Please advise because I will make you all say and not what I want to do. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC From rjbuesa <@t> yahoo.com Fri Jun 3 15:02:50 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 3 15:02:54 2011 Subject: [Histonet] Slide Bright Question In-Reply-To: Message-ID: <54202.28663.qm@web65710.mail.ac4.yahoo.com> General advise: To dewax sections before staining I used 2% aqueous solution of dishwasher soap at 90?C (2 changes)?with admirable results. You go directly to water ? stain. After staining, I just dried the stained sections in an oven at 60?C for 5 minutes ? coverslip. Try it. Ren? J. --- On Fri, 6/3/11, Debra Siena wrote: From: Debra Siena Subject: RE: [Histonet] Slide Bright Question To: "Christopher Conlisk" , "histonet@lists.utsouthwestern.edu" Date: Friday, June 3, 2011, 2:31 PM Hi Christopher, There are differences between how Xylene substitutes works and Xylene.? First of all, Xylene subs can be a bit slower to deparaffinize, so you may need to increase the time or # or stations when deparaffinizing.? Also, Xylene subs don't tolerate water so your last alcohol must be 100% or else you will see the eosin bleed out of the sections.? The other thing about Xylene substitutes is that the mounting media must be compatible with the substitute.???If I can help in any other way, please let me know.? thanks Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products Direct: 972-436-1010? x229 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christopher Conlisk Sent: Friday, June 03, 2011 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Bright Question Hello Everyone, ? ? I have worked in labs that use Xylene my entire career and I just started at a Lab That only uses a Xylene Substitute "Slide-Bright". I am having problems with the H&E. After staining and coverslipping (The slides look fine innitially), Then about 5-10 minutes after coverslipping the Eosin starts bleeding out all around the tissue. I have asked several of my Histotech Friends that are old timers and they say that Xylene Substitutes are awful at deparrifinization and awful at clearing. They told me that the alcohol isnt getting thoroughly cleared in the "Slide Brite" and then it is eventually leeching out after coverslipping??? Is this true and does anyone have any guidance for this issue? We also run MOHS slides on the same stainer and I keep all the reagents clean as a whistle. I really hate Xylene Substitute's.... Thanks C.S. Conlisk HT(ASCP), PBT(ASCP) Kansas City Skin and Cancer Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Fri Jun 3 15:33:00 2011 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Fri Jun 3 15:33:06 2011 Subject: [Histonet] why no replay??--Alcian Blue In-Reply-To: <481818.50221.qm@web112605.mail.gq1.yahoo.com> References: <481818.50221.qm@web112605.mail.gq1.yahoo.com> Message-ID: According to Dako, At pH 2.5, Alcian Blue stains sulphated and non-sulphated acidic carbohydrates. At pH 1.0, only sulphated carbohydrates are stained. Jerry RicksResearch Scientist University of WashingtonDepartment of Pathology > Date: Fri, 3 Jun 2011 12:21:31 -0700 > From: k84as@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] why no replay?? > > i have posted my quistion 2-3 times several days ago and recieve no negative or positive comment !!! why?? > is my quistion not clear or what?? > > --- On Thu, 6/2/11, mohamed abd el razik wrote: > > > From: mohamed abd el razik > Subject: Alcian blue quistion > To: histonet@lists.utsouthwestern.edu > Date: Thursday, June 2, 2011, 10:53 AM > > > > > > > > > > > > > > > > hi every body > i have a confusing quistion reagrding Alcian blue pH 1 & 2.5 stains. > suppose you have 100 goblet cell react positive to pH 1 which mean all have sulfated mucin. and 50 cell from other section react positive to pH 2.5 which mean acidic type(sulfated or sialated). why these 50 cell only react to pH 2.5 althogh the other non reacted cells possess acidic sulfated type?? on other word i need to know for what type of mucins alcian B 2.5 react with? > > thanx all > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Jun 3 17:51:02 2011 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jun 3 17:51:15 2011 Subject: [Histonet] Re: Processing mouse pancreas Message-ID: <000001cc2240$b8afc4c0$2a0f4e40$@callis@bresnan.net> It was written: Develop a good general protocol for animal tissue. You cannot have "dedicated" protocols for every type of tissue. Ren? J. --- On Fri, 6/3/11, Stoll, Kathryn <@t> mcw.edu> wrote: From: Stoll, Kathryn <@t> mcw.edu> Subject: [Histonet] Processing Mouse Pancreas To: "histonet <@t> lists.utsouthwestern.edu" <@t> lists.utsouthwestern.edu> Date: Friday, June 3, 2011, 12:03 PM Happy Friday Everyone, Does anyone have a protocol for processing mouse pancreas? We have a VIP6. I am not familiar with the processing any animal tissue and do not know if it is similar to human. Thanks in advance. Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kstoll <@t> mcw.edu **************************************************************************** **************************************** Sorry, but I couldn't disagree more about developing an overal(?)l general protocol for animal tissue. This may work for diagnostic veterinary laboratories where daily runs require a single overnight schedule, but in a research laboratories where one works almost exclusively with the mouse or other rodent animal models, then different or custom processing schedules are common. This is the case with our research laboratory. We avoid "over processing" or over exposure to solvents, effects of clearing agents and paraffin heat as much as possible and find different schedules work better depending on size and type of tissue being processed. Mouse pancreas is very small, so avoid "over processing" e.g. lengthy processing in each solvent, clearing agent and hot paraffin to not dry out the tissue resulting is difficult sectioning. For pancreas or other small, delicate murine tissues, I suggest you use a short schedule. This is much like a biopsy schedule found in clinical laboratories. In fact, you can use your biopsy schedule or at least compare it to the following short schedule. We have a VIP6, an extremely efficient machine compared to our old VIP, and had to readjust/shorten the processing times for spleen, liver, single lung lobes, lymph nodes, spinal cords cord cross sections, tiny embryos, etc. Some researchers have as many as three to four different schedules depending on what organs they are processing. One researcher has three custom schedules for brain and spinal cord depending on whether the brain is whole, sliced or spinal cord is in cross sections. For whole lungs, or larger tissues, we use a longer schedule probably similar to your routine clinical schedule, and for bone, much longer processing due to density of the decalcified bone matrix. We have used the following short schedule with success, and this should work for the pancreas. Tissue should be totally fixed before putting on the processor since all processing starts 70% ethanol. If formalin is on the machine, then one can use the processor setup which will probably have two changes of 95% and 100% alcohols, and increase the times accordingly to ensure good dehydration. We use pressure and vacuum, no heat added to any solvents (heat lends to more drying of lean animal tissue). 70% ETOH 15 minutes 80% ETOH 15 minutes 95% ETOH (3 changes) 15 minutes each - if two stations, use 25 minutes per change 100% ETOH (3 changes) 15 minutes each - if two stations, use 25 minutes per change Xylene (1 change) 15 - 20 minutes Clearite 3 (1 change) 15 - 20 minutes Paraffin at 60?C (3 changes) 20 minutes per change. You can use two xylene changes if you already have that on your VIP6. You can also use Clearite 3, Propar or another xylene substitute exclusively but we avoid limonene based xylene substitutes (sensitizing and makes people sick due annoying orange citrus odor), but increase the time (20 - 25 minutes) per change in the substitutes. These are more sensitive to residual water carry over. We like to ensure the alcohols with any residual traces of water are cleared well with xylene in first step, and then finish with Clearite 3 in second step. Clearite 3 or Propar doesn't harden the tissue excessively making sectioning easier. Paraffin choice is whatever the lab prefers. IF you find you need longer processing, increase the time by 5 minutes in each change including the paraffin. Good luck Gayle M. Callis HTL/HT/MT(ASCP) GCallis Histology Service LLC Bozeman MT From k84as <@t> yahoo.com Sat Jun 4 11:00:10 2011 From: k84as <@t> yahoo.com (mohamed abd el razik) Date: Sat Jun 4 11:00:16 2011 Subject: [Histonet] why no replay???? Message-ID: <905023.42575.qm@web112609.mail.gq1.yahoo.com> thanx to all kind replays from sgoebel,?Paula Pierce and JR R. but i know that Alcian blue 2.5 stain most acidic mucins and pH 1 stain sulfated one. my quistion is: ?i found in several serial section on intestine that 100 goblet cell react to AB ph?1 and only 50 cell reacts to AB 2.5 why?????? it should be the reverse numbers as at pH 2.5 all acidic sulfated or non sulfated are stained. in other words, ph 2.5 should stain 100 cell as ph 1 stains 100 cell at least. so i'm confused about that and replay or staining several times according to Bancroft and Farida?and these was confirmed From wilson6848 <@t> yahoo.com Sat Jun 4 21:55:22 2011 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Sat Jun 4 21:55:26 2011 Subject: [Histonet] Looking for Position in IHC Message-ID: <599892.73522.qm@web120905.mail.ne1.yahoo.com> ?? ?I am an HTL(ASCP)QIHC Certified Histotech with an extensive experience in IHC. I am looking for position in IHC.? Willing to relocate. ?Thanks,?Wilson. From histotech <@t> imagesbyhopper.com Sun Jun 5 05:58:56 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sun Jun 5 05:59:06 2011 Subject: [Histonet] Submission of tonsils to pathology In-Reply-To: <25A4DE08332B19499904459F00AAACB71987E03D8B@EVS1.archildrens.org> References: <4DE8CBA8.7400.0077.1@harthosp.org> <231345.32485.qm@web65716.mail.ac4.yahoo.com> <25A4DE08332B19499904459F00AAACB71987E03D8B@EVS1.archildrens.org> Message-ID: <0E28579D-165E-46F4-ACAE-B0A9D4E7893E@imagesbyhopper.com> We do micros on every one of them. On Jun 3, 2011, at 12:13 PM, "Horn, Hazel V" wrote: > Generally all are gross only. There are some exceptions depending on patient history or if one is significantly larger than the other the pathologists have the discretion to do a microscopic. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Autopsy/Histology/Transcription > Arkansas Children's Hospital > 1 Children's Way Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Friday, June 03, 2011 11:11 AM > To: Histonet; Richard Cartun > Subject: Re: [Histonet] Submission of tonsils to pathology > > Labelled "gross only". > Ren? J. > > --- On Fri, 6/3/11, Richard Cartun wrote: > > > From: Richard Cartun > Subject: [Histonet] Submission of tonsils to pathology > To: "Histonet" > Date: Friday, June 3, 2011, 11:55 AM > > > What is your policy on the submission of tonsils to pathology for examination (gross or microscopic)? Thank you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Sun Jun 5 12:01:26 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Jun 5 12:01:34 2011 Subject: AW: [Histonet] alcianblue riddle In-Reply-To: <905023.42575.qm@web112609.mail.gq1.yahoo.com> References: <905023.42575.qm@web112609.mail.gq1.yahoo.com> Message-ID: <32F2B33B900B4CD48EE014B6FC8D7297@dielangs.at> Have you checked the pH in the coplin jars? Perhaps someone has labelled them wrong? Or is there any dilution-effect while staining, that raises the pH? I think about covering tapwater-wet slides with little amount of dye-solution? Or the acetic acid is too old, so the right pH isn't reached from the beginning? Gudrun -----Ursprüngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von mohamed abd el razik Gesendet: Samstag, 04. Juni 2011 18:00 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] why no replay???? thanx to all kind replays from sgoebel, Paula Pierce and JR R. but i know that Alcian blue 2.5 stain most acidic mucins and pH 1 stain sulfated one. my quistion is:  i found in several serial section on intestine that 100 goblet cell react to AB ph 1 and only 50 cell reacts to AB 2.5 why?????? it should be the reverse numbers as at pH 2.5 all acidic sulfated or non sulfated are stained. in other words, ph 2.5 should stain 100 cell as ph 1 stains 100 cell at least. so i'm confused about that and replay or staining several times according to Bancroft and Farida and these was confirmed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Milton.Gomez <@t> nyumc.org Sun Jun 5 12:09:21 2011 From: Milton.Gomez <@t> nyumc.org (Gomez, Milton) Date: Sun Jun 5 12:09:25 2011 Subject: [Histonet] Distribution of work for older generation of Histotechs Message-ID: <4A53F9A1D7C2674FA4A6E650D703DDA5FB74FBAD@MSGWSDCPMB07.nyumc.org> Hello Histonetters, Is the distribution of work different for older histotechs vs. younger histotechs in your labs and why? Do they get special assignments or duties because of their growing wisdom and seniority? Thanks in advance, MG ------------------------------------------------------------
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From pruegg <@t> ihctech.net  Sun Jun  5 13:02:44 2011
From: pruegg <@t> ihctech.net (pruegg@ihctech.net)
Date: Sun Jun  5 13:02:49 2011
Subject: [Histonet] Distribution of work for older generation of Histotechs
Message-ID: <20110605110244.f86bd30e73b823f57b516b5451216a98.d819ee2dbc.wbe@email00.secureserver.net>


   that  is  a  very interesting thread.  I am a senior histotech    own  my own Histopathology Services Business so i get to decide w   everyone does including me.  I tend to do most of the QC/QA work a   management  these days but i do not hesitate to work at the bench when
   ne   hundreds  of   i have to.



   Regards,

   Patsy



   -------- Original Message --------
   Subject: [Hi   Histotechs
   From:   Date: Sun, June 05, 2011 10:09 am
   To: "'[2]histonet@lists.utsouthwestern.ed   <[3]histone   Hello Histonetters,
   Is  the   younger  histotechs   assignments or duties because of   Thanks in advance,
   MG
-------------------------- This email message, inclu of the intended recipient(s) and proprietary, confidential, and exempt from applicable law. Any unauthorized review, use, disclosure, distribution is prohibited. If you have received this email in error ple message. Plea attachments for the liability for any damage c email.
==== ======================= 3D= 
   __________   Histonet mailing list
   [4]Histonet@lists.utsouthwestern   [5]http://lists.utsouthwestern.edu/mailman/listinfo/histonet

References

   1. 3D"mailto:Milton.Gomez@nyumc.org"
   2. file://localhost/tmp/3D   3. 3D"mailto:histonet@lists.utsouthwestern.edu"
   4. ="mailto:Histonet@lists.utsouthwestern.edu"
   5. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/his
From AnthonyH <@t> chw.edu.au  Sun Jun  5 18:12:53 2011
From: AnthonyH <@t> chw.edu.au (Tony Henwood)
Date: Sun Jun  5 18:13:09 2011
Subject: [Histonet] Submission of tonsils to pathology
In-Reply-To: <4DE8CBA8.7400.0077.1@harthosp.org>
References: <4DE8CBA8.7400.0077.1@harthosp.org>
Message-ID: <6D6BD1DE8A5571489398B392A38A7157188594C0@xmdb02.nch.kids>

Hi Richard,

Our policy is to receive all tissues removed at surgery. If microscopy is not required then they are treated as an "audit" biopsy, accessioned and a macroscopic description done.
A report stating such is sent to the patient's notes. The specimens are then retained for the legislative period - 1 month from date of issue of specimen report (Australian NPAAC Standard 2009). In our department we keep them for 3 months.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Saturday, 4 June 2011 1:55 AM
To: Histonet
Subject: [Histonet] Submission of tonsils to pathology

What is your policy on the submission of tonsils to pathology for examination (gross or microscopic)?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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From AnthonyH <@t> chw.edu.au  Sun Jun  5 18:22:42 2011
From: AnthonyH <@t> chw.edu.au (Tony Henwood)
Date: Sun Jun  5 18:23:02 2011
Subject: [Histonet] Submission of tonsils to Pathology - #2
In-Reply-To: <683621D7852C2F488898D0AC7F164A98882DC3E9@CMCPBEXMAIL02.Childrens.med>
References: <4DE8D0F1020000770002764F@gwmail1.harthosp.org> 
	<4DE8D3FE0200007700027658@gwmail1.harthosp.org> 
	<4DE8D64F020000770002765B@gwmail1.harthosp.org> 
	<4DE8D64E.7400.0077.0@harthosp.org> 
	<683621D7852C2F488898D0AC7F164A98882DC3E9@CMCPBEXMAIL02.Childrens.med>
Message-ID: <6D6BD1DE8A5571489398B392A38A7157188594E2@xmdb02.nch.kids>

I suppose our concern is that theatre staff may inadvertently throw away the wrong specimen (eg diagnostic skin, etc). If they are not given the chance to make a choice then there is no chance of miss-communication. 
Since we are not doing microscopy on these specimens, there is minimal increase in workload. 
The small pots that the tonsils arrive in did not affect our storage capacity. 
So in our situation it was not a big deal to accept these specimens.
But this might not be feasible for all labs.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf
Sent: Saturday, 4 June 2011 4:08 AM
To: 'Richard Cartun'; Histonet
Subject: RE: [Histonet] Submission of tonsils to Pathology - #2

Richard;
Our children's Hospital (Children's Medical Center Dallas where I am the lab's Medical Director) made tonsils exempt from required pathologic examination 8 or so years ago following approval of the medical and surgical staff.  We now only receive tonsils the surgeons are concerned about (transplant patients, asymmetric lesions etc.).  Our surgical case accession numbers dropped by about 800 as a result of this but we do feel we are doing what's most cost effective yet beneficial to the patient.  We get one or two cases a week now. We have about 15 other items on our list of specimens that are exempt from pathologic review.  It is important to have the pathologists work with the clinicians to generate the list of tissue specimens that don't need to come to pathology.  
Linda M
Histonet administrator	

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Friday, June 03, 2011 11:41 AM
To: Histonet
Subject: [Histonet] Submission of tonsils to Pathology - #2

I apologize; I should have stated my question differently.  Our Children's Hospital wants to stop sending tonsils to Pathology unless the surgeon requests pathological examination.  An article published in Otolaryngology-Head and Neck Surgery (Nelson et al.) concluded that pathologic examination of tonsils is not a cost-effective use of limited health care resources.  "Zero cases of unsuspected pathology were identified on planned gross specimen evaluation (n=4186) and positive pathologic findings on microscopic analysis (n=1066) were only identified in post-transplant patients."

Have any of you exempted tonsils from submission to Pathology?  If not, maybe we should.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



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From whitmorel <@t> mindspring.com  Sun Jun  5 14:28:05 2011
From: whitmorel <@t> mindspring.com (whitmorel@mindspring.com)
Date: Sun Jun  5 18:23:28 2011
Subject: [Histonet] Hey histonet
Message-ID: 

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From ltougas <@t> dawsoncollege.qc.ca  Sun Jun  5 18:32:44 2011
From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas)
Date: Sun Jun  5 18:33:39 2011
Subject: [Histonet] Hey histonet
In-Reply-To: 
References: 
Message-ID: <7618BC6D53F39149B7B57615C218AADF239DE1DAC8@EXCHANGE.ad.dawsoncollege.qc.ca>

Please refrain from bothering us with commercial offers; this is not the right place.

________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of whitmorel@mindspring.com [whitmorel@mindspring.com]
Sent: June 5, 2011 3:28 PM
To: histonet
Subject: [Histonet] Hey histonet

histonet hey, I just wanted to share this opportunity with you, I've been making 200-300 dollars a day and I started only a week ago. Check out this news article and it will show you how to get started, it's definitely easy enough for you :)! http://t.co/vsr9jMJ


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From suhyoung.jeong <@t> gmail.com  Mon Jun  6 07:38:34 2011
From: suhyoung.jeong <@t> gmail.com (Suhyoung Jeong)
Date: Mon Jun  6 07:38:39 2011
Subject: [Histonet] Pre-made paraformaldehyde question
Message-ID: 

Dear Histo people,

In my old lab, I have been taught that 4% PFA has to be always made fresh
(maybe a day before max). Here in my new lab, I find that people use 32%
pre-made PFA from EMS (without expiry date) and only dilute it on the day of
use.

Does anyone have experience (or thoughts) with this issue?

Thank you in advance

All the best, Suh
From mw <@t> personifysearch.com  Mon Jun  6 08:29:42 2011
From: mw <@t> personifysearch.com (Matt Ward)
Date: Mon Jun  6 08:29:49 2011
Subject: [Histonet] JOB ALERT - NEW Field Histology Opportunity Based in
	North Carolina!!!
Message-ID: 

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*Location: North Carolina*







The position offers a very strong Base Salary + Bonus + Car Allowance + Full
Benefits.  This position does not require previous field experience and is
perfect for a histotech who is interested in opportunities outside of the
lab.







Please contact me directly at mw@personifysearch.com to learn more.







Regards,







Matt Ward

*Account Executive*

*Personify*

5020 Weston Parkway Suite 315

Cary NC 27513

(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

 www.personifysearch.com













Field Support Specialist





The Company:



Our client is a leading developer and producer of innovative high-tech
precision optics systems for the analysis of microstructures.  As one of the
market leaders in each of the fields of Microscopy, Confocal Laser Scanning
Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment.

Comprising nine manufacturing facilities in seven countries, sales and
service companies in 20 countries and an international network of dealers,
the company is represented in over 100 countries.



Primary Responsibilities:



The primary responsibility of this role will be to provide in-field
technical applications support for current and next generation range of
products.  The FSS will install/set-up instrumentation in customer
laboratories, perform demonstrations and maintain demonstration equipment in
a clean and operational manner.  The FSS will also train customers on the
use of instrumentation.  Conducting in-field post purchase applications
support and performing instrument validations will be a key responsibility
of this position.



Additional Responsibilities:

- Build an in-depth understanding of new product technologies and their
applications in a diagnostic environment

- Contribute to the strategic planning process by providing technical data
on instrumentation and reagents

- Conduct post-purchase reagent order management

- Be the customer liaison by providing scientific and laboratory expertise
for in-field installations, evaluations, training and trouble-shooting

- Provide customer support for remote problem solving

- Design and perform experiments to investigate and solve tough technical
applications problems



Matt Ward

*Account Executive*

*Personify*

5020 Weston Parkway Suite 315

Cary NC 27513

(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

 www.personifysearch.com
From PAMarcum <@t> uams.edu  Mon Jun  6 08:51:52 2011
From: PAMarcum <@t> uams.edu (Marcum, Pamela A)
Date: Mon Jun  6 08:51:56 2011
Subject: [Histonet] RE: Distribution of work for older generation of
	Histotechs
In-Reply-To: <4A53F9A1D7C2674FA4A6E650D703DDA5FB74FBAD@MSGWSDCPMB07.nyumc.org>
References: <4A53F9A1D7C2674FA4A6E650D703DDA5FB74FBAD@MSGWSDCPMB07.nyumc.org>
Message-ID: 

We make every attempt to have all of the histologist at UAMS do the same rotations and jobs no matter the age.  It is sometimes difficult due to some internal changes in the locations of the Gross Room and Histology (literally a quarter mile apart) however; we all work with what we have in these areas.   Occasionally someone will object to doing a job and it is explained we all do everything as we are short handed and have no choice.

Pam Marcum

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gomez, Milton
Sent: Sunday, June 05, 2011 12:09 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Distribution of work for older generation of Histotechs

Hello Histonetters,

Is the distribution of work different for older histotechs vs. younger histotechs in your labs and why? Do they get special assignments or duties because of their growing wisdom and seniority?

Thanks in advance,

MG
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From Vickroy.Jim <@t> mhsil.com  Mon Jun  6 10:43:15 2011
From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim)
Date: Mon Jun  6 10:43:23 2011
Subject: [Histonet] New Workflow Model
Message-ID: <24A4826E8EF0964D86BC5317306F58A55DF5E8CD0C@mmc-mail.ad.mhsil.com>

We are definitely looking at making some changes to improve turnaround time and workflow.   Currently I have staff at work from 0400 - 1730 each day.  We have conventional tissue processors and also a microwave tissue processor.   I have been in the field for 32 years and of course  am an "old dog" but still think I am capable of learning new tricks.  We are in the process of changing our pathology system to CoPath plus and will be also making some moves to expand.  I am being strongly encouraged to look at this time also to make some changes in our workflow to decrease turnaround time.  Obviously that is a lot on our plate at the same time however it seems reasonable that some of the changes can be made simultaneously since some of our workflow processes will be changed automatically because of the CoPath bar coded tracking system that will occur during the implementation of CoPath.   We are considering looking into a consultant to help us with these changes but I wondered if anyone could shed a little light on their experiences of changes they have made.




James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046


________________________________
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From Jackie.Fleming <@t> allina.com  Mon Jun  6 11:32:19 2011
From: Jackie.Fleming <@t> allina.com (Fleming, Jackie M)
Date: Mon Jun  6 11:32:32 2011
Subject: [Histonet] block disposal
Message-ID: <2342C8D836A0954199F6D58A44BDD1BEB240@XCHMB01.allina.com>

How do facilities dispose of large amounts of paraffin blocks after the after the CAP 10 year guidelines?



Jackie Fleming HT ASCP

Allina Medical Laboratory

Histology Technical Consultant

phone:612-863-4773

Internal Zip 11136

This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message.


From bakevictoria <@t> gmail.com  Mon Jun  6 11:42:46 2011
From: bakevictoria <@t> gmail.com (Victoria Baker)
Date: Mon Jun  6 11:42:49 2011
Subject: [Histonet] Special stains poster
Message-ID: 

 Hi

I think it was about six or seven years ago I had come across a company that
made a 'promotional' laminated poster of widely used special stains that
they had given to customers to show new techs and students how stains should
"look".  I've had no luck in searching the internet so I'm hoping someone o
the histonet can help me.  I've got a group of tech's I'm working with that
have varying levels of experience and a poster like this would be a big help
to them.

If anyone has a source, please let me know.

Thank you in advance.

Vikki
From dellav <@t> musc.edu  Mon Jun  6 11:44:46 2011
From: dellav <@t> musc.edu (Della Speranza, Vinnie)
Date: Mon Jun  6 11:45:54 2011
Subject: [Histonet] Real Pathologist of Genius
In-Reply-To: <32F2B33B900B4CD48EE014B6FC8D7297@dielangs.at>
References: <905023.42575.qm@web112609.mail.gq1.yahoo.com>
	<32F2B33B900B4CD48EE014B6FC8D7297@dielangs.at>
Message-ID: 

I've not seen this posted here. I apologize if this is a repeat.

If you've not seen this, it is a MUST SEE, especially if you do IHC
You'll need speakers

http://www.youtube.com/watch?v=-fsn0GrzX5U&feature=player_embedded


Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue Suite MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974

From asmith <@t> mail.barry.edu  Mon Jun  6 12:51:54 2011
From: asmith <@t> mail.barry.edu (Smith, Allen)
Date: Mon Jun  6 12:52:05 2011
Subject: [Histonet] RE: block disposal
In-Reply-To: <2342C8D836A0954199F6D58A44BDD1BEB240@XCHMB01.allina.com>
References: <2342C8D836A0954199F6D58A44BDD1BEB240@XCHMB01.allina.com>
Message-ID: 

The best solution would be to give them to the Cooperative Human Tissue Network.
Allen A. Smith
Professor of Anatomy
Barry University School of Podiatric Medicine
Miami Shores, Florida

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fleming, Jackie M
Sent: Monday, June 06, 2011 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] block disposal

How do facilities dispose of large amounts of paraffin blocks after the after the CAP 10 year guidelines?



Jackie Fleming HT ASCP

Allina Medical Laboratory

Histology Technical Consultant

phone:612-863-4773

Internal Zip 11136

This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message.


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From Wanda.Smith <@t> HCAhealthcare.com  Mon Jun  6 13:18:51 2011
From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com)
Date: Mon Jun  6 13:18:56 2011
Subject: [Histonet] RE: block disposal
In-Reply-To: <2342C8D836A0954199F6D58A44BDD1BEB240@XCHMB01.allina.com>
References: <2342C8D836A0954199F6D58A44BDD1BEB240@XCHMB01.allina.com>
Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A7743C7A@NADCWPMSGCMS03.hca.corpad.net>

We dispose of them in double red bags and incineration boxes to be hauled to the incinerator.

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fleming, Jackie M
Sent: Monday, June 06, 2011 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] block disposal

How do facilities dispose of large amounts of paraffin blocks after the after the CAP 10 year guidelines?



Jackie Fleming HT ASCP

Allina Medical Laboratory

Histology Technical Consultant

phone:612-863-4773

Internal Zip 11136

This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message.


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From MSHERWOOD <@t> PARTNERS.ORG  Mon Jun  6 13:25:09 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Mon Jun  6 13:25:13 2011
Subject: [Histonet] RE: block disposal
In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A7743C7A@NADCWPMSGCMS03.hca.corpad.net>
References: <2342C8D836A0954199F6D58A44BDD1BEB240@XCHMB01.allina.com>
	<9E2D36CE2D7CBA4A94D9B22E8328A3BA13A7743C7A@NADCWPMSGCMS03.hca.corpad.net>
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB57BC@PHSXMB30.partners.org>

We do the same. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Wanda.Smith@HCAhealthcare.com
Sent: Monday, June 06, 2011 2:19 PM
To: Jackie.Fleming@allina.com; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: block disposal

We dispose of them in double red bags and incineration boxes to be hauled to the
incinerator.

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or
CONFIDENTIAL information and may be read or used only by the intended recipient.
If you are not the intended recipient of the email or any of its attachments,
please be advised that you have received this email in error and that any use,
dissemination, distribution, forwarding, printing, or copying of this email or
any attached files is strictly prohibited. If you have received this email in
error, please immediately purge it and all attachments and notify the sender by
reply email or contact the sender at the number listed.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fleming, Jackie
M
Sent: Monday, June 06, 2011 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] block disposal

How do facilities dispose of large amounts of paraffin blocks after the after
the CAP 10 year guidelines?



Jackie Fleming HT ASCP

Allina Medical Laboratory

Histology Technical Consultant

phone:612-863-4773

Internal Zip 11136

This message contains information that is confidential and may be privileged.
Unless you are the addressee (or authorized to receive for the addressee), you
may not use, copy or disclose to anyone the message or any information contained
in the message. If you have received the message in error, please advise the
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


From jennifer.harvey <@t> Vanderbilt.Edu  Mon Jun  6 13:35:00 2011
From: jennifer.harvey <@t> Vanderbilt.Edu (Harvey, Jennifer Lynn)
Date: Mon Jun  6 13:35:04 2011
Subject: [Histonet] Pie crust effect on frozen Muscle H&E
Message-ID: 

Has anyone ever heard of ?pie crust? effect on frozen muscle slides? My pathologist has found the picture in a book calling our artifact ?pie crust? but it gave no explanation as to the cause or fix. Help!

Jennifer Harvey, HT(ASCP) QIHC
Vanderbilt University Medical Center
Neuropathology Lab Supervisor
C-2309 Medical Center North
Nashville, TN  37232-2561
Phone: 615-343-0083
Fax: 615-343-7089

From candice_camille <@t> yahoo.com  Mon Jun  6 13:42:33 2011
From: candice_camille <@t> yahoo.com (Candice Smoots)
Date: Mon Jun  6 13:42:38 2011
Subject: [Histonet] polyvinyl alcohol mouting medium
Message-ID: <446969.78928.qm@web125414.mail.ne1.yahoo.com>

Hi guys,

?I was wondering if someone could tell me about this?mounting medium. I am 
allowing it to unthaw and it seems that it will not form into a liquid. Is this 
normal. I have tries running it under warm water( well covered of course). Is 
this supposed to be a liquid or?am I supposed to use it congealed. Any advice is 
appreciated.
?I remain yours truely, 

Candice Camille  
From relia1 <@t> earthlink.net  Mon Jun  6 13:44:21 2011
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Mon Jun  6 13:44:22 2011
Subject: [Histonet] RELIA Hot Histology Job Alert 6/6/2011 Histology
	Supervisor for Leading Nashville, TN  area Private Lab
Message-ID: <707CF19ACF8C4AF4AC7D3FE30DEB5524@ownerf1abaad51>

Hi Histonetters!!
I hope everyone has had a great start to their week.  I just wanted to
put up a quick post about a brand new position I am working on and am
pretty excited about.  My client is a private lab located in the greater
Nashville area and they have asked me to assist  them in their search
for a histology supervisor.  They need someone with 3-5 years of
experience in all aspects of routine histology combined with experience
supervising a histology lab.  This is a dayshift full time permanent
position and my client offers an excellent salary, great benefits and
relocation assistance.  If you or anyone you know might be interested in
more information please contact me at relia1@earthlink.net or toll free
at 866-607-3542.  Thanks again - Pam

Thank You!
 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.com  search Pam Barker RELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 

From JMacDonald <@t> mtsac.edu  Mon Jun  6 13:56:24 2011
From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald)
Date: Mon Jun  6 13:53:51 2011
Subject: [Histonet] block disposal
In-Reply-To: <2342C8D836A0954199F6D58A44BDD1BEB240@XCHMB01.allina.com>
Message-ID: 

Or if there is no patient identifiers donate them to a histology school 
for practice blocks.




"Fleming, Jackie M"  
Sent by: histonet-bounces@lists.utsouthwestern.edu
06/06/2011 09:33 AM

To
"histonet@lists.utsouthwestern.edu" 
cc

Subject
[Histonet] block disposal






How do facilities dispose of large amounts of paraffin blocks after the 
after the CAP 10 year guidelines?



Jackie Fleming HT ASCP

Allina Medical Laboratory

Histology Technical Consultant

phone:612-863-4773

Internal Zip 11136

This message contains information that is confidential and may be 
privileged. Unless you are the addressee (or authorized to receive for the 
addressee), you may not use, copy or disclose to anyone the message or any 
information contained in the message. If you have received the message in 
error, please advise the sender by reply e-mail and delete the message.


_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From rjbuesa <@t> yahoo.com  Mon Jun  6 13:59:15 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Mon Jun  6 13:59:18 2011
Subject: [Histonet] block disposal
In-Reply-To: <2342C8D836A0954199F6D58A44BDD1BEB240@XCHMB01.allina.com>
Message-ID: <214477.1762.qm@web65713.mail.ac4.yahoo.com>

I incinerate them.
Ren? J.

--- On Mon, 6/6/11, Fleming, Jackie M  wrote:


From: Fleming, Jackie M 
Subject: [Histonet] block disposal
To: "histonet@lists.utsouthwestern.edu" 
Date: Monday, June 6, 2011, 12:32 PM


How do facilities dispose of large amounts of paraffin blocks after the after the CAP 10 year guidelines?



Jackie Fleming HT ASCP

Allina Medical Laboratory

Histology Technical Consultant

phone:612-863-4773

Internal Zip 11136

This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message.


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From ArtimK <@t> slhn.org  Mon Jun  6 14:30:33 2011
From: ArtimK <@t> slhn.org (Artim, Kimberly)
Date: Mon Jun  6 14:30:38 2011
Subject: [Histonet] Fite control block
Message-ID: <9E67FDD215B226448638018A82B952BD021E7261D5BA@EXCHANGE.slhn.org>

Does anyone know where I can get a Fite control block?

Kimberly Artim, AST, HT (ASCP)
Technical Coordinator, Anatomic Pathology
St Lukes Hospital & Health Network
801 Ostrum Street
Bethlehem, PA 18015
610-954-4832


Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information.  Any unauthorized review, use, disclosure or distribution is prohibited.  If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
From c.tague <@t> pathologyarts.com  Mon Jun  6 14:46:18 2011
From: c.tague <@t> pathologyarts.com (Curt Tague)
Date: Mon Jun  6 14:46:36 2011
Subject: [Histonet] Fite control block
In-Reply-To: <9E67FDD215B226448638018A82B952BD021E7261D5BA@EXCHANGE.slhn.org>
References: <9E67FDD215B226448638018A82B952BD021E7261D5BA@EXCHANGE.slhn.org>
Message-ID: <053501cc2482$673fbac0$35bf3040$@tague@pathologyarts.com>

They're hard to come by but I'm sure there is a few helpful and kind people
out here that will help you out. I've helped a few people out with other
stuff in the past and people have helped me. It's like we're all one big
happy family helping each other when in need.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Artim,
Kimberly
Sent: Monday, June 06, 2011 12:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fite control block

Does anyone know where I can get a Fite control block?

Kimberly Artim, AST, HT (ASCP)
Technical Coordinator, Anatomic Pathology
St Lukes Hospital & Health Network
801 Ostrum Street
Bethlehem, PA 18015
610-954-4832


Confidentiality Notice: This e-mail message, including any attachments, is
for the sole use of intended recipient(s) and may contain confidential and
privileged information.  Any unauthorized review, use, disclosure or
distribution is prohibited.  If you are not the intended recipient, please
contact the sender by reply e-mail and destroy all copies of the original
message.
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From faith14913 <@t> aol.com  Mon Jun  6 19:02:08 2011
From: faith14913 <@t> aol.com (faith14913@aol.com)
Date: Mon Jun  6 19:02:20 2011
Subject: [Histonet] alcian blue 2.5 and derm specimens
Message-ID: <8CDF2BD4728F7E0-208C-44804@webmail-m148.sysops.aol.com>


We are having a problem with our alcian blue pH2.5 stain.  It is taining the collagen really dark and the mucin really light.  My boss just handed me this assignment today and told me to find out what is wrong with it, however I am not very familiar with this stain.  He suggested I remake all solutions fresh and try a known positive control, but I just wanted to check and see what "histoland" thought.

Also for derms, the epidermis on all of our squares and incisions are rolling up on the tissue during processing.  It happens when we use the VIP and the Pathos (microwave) processors.  I however have never done derms before this job so am also not very familiar with it....any help???  Thanks!



From LSebree <@t> uwhealth.org  Mon Jun  6 21:27:51 2011
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Mon Jun  6 21:36:54 2011
Subject: [Histonet] Real Pathologist of Genius
References: <905023.42575.qm@web112609.mail.gq1.yahoo.com><32F2B33B900B4CD48EE014B6FC8D7297@dielangs.at>
	
Message-ID: 

I'm not a beer drinker (Milwaukee born German, go figure) but I loved this!

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Della Speranza, Vinnie
Sent: Mon 6/6/2011 11:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Real Pathologist of Genius



I've not seen this posted here. I apologize if this is a repeat.

If you've not seen this, it is a MUST SEE, especially if you do IHC
You'll need speakers

http://www.youtube.com/watch?v=-fsn0GrzX5U&feature=player_embedded


Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue Suite MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From khairedai <@t> yahoo.com  Tue Jun  7 07:02:49 2011
From: khairedai <@t> yahoo.com (Khaire Dai)
Date: Tue Jun  7 07:02:52 2011
Subject: [Histonet] Fibroblasts Floating on Medium after 24 hrs.
Message-ID: <783046.65562.qm@web113014.mail.gq1.yahoo.com>

Hi
?
I have started growing the human normal and cancer associated fibroblasts.
?
The cells I got are from the vials preserved in liquid nitrogen and have seeded the cells on culture plates. Now after 24 hrs only very few cells are attached to the plate almost all of them are floating.
?
How long should i wait for the cells to attach or remove the floating cells?
I don't want to loose them as i dont have any backup for these cells.
?
Thanks in advance for the support.
From alisha <@t> ka-recruiting.com  Tue Jun  7 08:20:48 2011
From: alisha <@t> ka-recruiting.com (Alisha Dynan)
Date: Tue Jun  7 08:20:52 2011
Subject: [Histonet] Histology Job Opportunities
Message-ID: <1849502346.1307452848115.JavaMail.cfservice@SL4APP4>



Hi Histonet Members,

 

I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm.  I help place Lab Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities?  We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. 




 

Histotech in Maine:



I am currently working on an amazing opportunity with one of largest most sophisticated labs in the Northeast. It is located in coastal Maine. This company is looking to hire on a Histotech.  This person should be HT(ASCP) or HTL(ASCP) certified. The compensation package is fantastic and includes health, dental, a retirement plan, and relocation assistance, if needed. If interested in learning more details, please email me at alisha@ka-recruiting.com.

 


Histotech in Upstate NY:



I am currently working on an amazing opportunity with one of the top universities in upstate NY. They are looking to hire on a Histotech.  This person should be HT(ASCP) or HTL(ASCP) certified. The compensation package is fantastic and includes health, dental, a retirement plan, and relocation assistance, if needed. If interested in learning more details, please email me at alisha@ka-recruiting.com.




 



Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates.


 















Cytotech and Histotech


1. Maine - Histotech


2. Upstate NY - Histotech


3. Florida - several Histotech positions


4. Georgia - Histotech


5. North Carolina - Histotech


6. North Carolina - Histology Supervisor


7. Georgia - Histology Supervisor


8. New York (Westchester County) - QA Supervisor


9. New York City - Histotech (very specialized position)


10. New York (Westchester County) - Cytotech


11. New York (Westchester County) - Grossing Supervisor


12. New York (Westchester County) - IHC Technologist


13. New York (Westchester County) - Manager of Laboratory Operations


14. New York (Westchester County) - Histotech



 























If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is.  

 

If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future.  I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country.  

 


To view some additional opportunities please visit our website at www.ka-recruiting.com. 












Sincerely,

 

Alisha Dynan, Founder

K.A. Recruiting, Inc.

Your Partner in Healthcare Recruiting

10 Post Office Square 8th Floor SOUTH

Boston, MA 02109

P: (617) 692-2949

F: (617) 507-8009

alisha@ka-recruiting.com

www.ka-recruiting.com

 




 



From alisha <@t> ka-recruiting.com  Tue Jun  7 08:43:00 2011
From: alisha <@t> ka-recruiting.com (Alisha Dynan)
Date: Tue Jun  7 08:42:53 2011
Subject: [Histonet] Histology Jobs
Message-ID: <111741420.1307454180034.JavaMail.cfservice@sl4app2>



Hi Histonet Members,


I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm.  I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities?  We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. 





 

I am currently working on a histotechnologist position with a fast paced and service-oriented company in New York, NY. This company is an innovative, commercial laboratory that specializes in performing and developing testing services that serve the technically advanced medical community with a focus on neurological disorders. They are looking to hire on for the following position:

 

   * Experienced Histotech - NYS licensed histotech, 5-10+ years experience, IHC experience a plus, experience with frozen sections a plus, experience in derm a plus

 


They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is.  

If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future.  I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country.  

 

We also have about 20 other histology job opportunities throughout the state of NY. 

If you're looking to relocate out of NY, I have histology job opportunities in many areas across the country.

Let me know if you would like to learn more.

To view some additional opportunities please visit our website at www.ka-recruiting.com. 






Sincerely,

 

Alisha Dynan, Founder

K.A. Recruiting, Inc.

Your Partner in Healthcare Recruiting

10 Post Office Square 8th Floor SOUTH

Boston, MA 02109

P: (617) 692-2949

F: (617) 507-8009

alisha@ka-recruiting.com

www.ka-recruiting.com

 




 



From mcauliff <@t> umdnj.edu  Tue Jun  7 08:59:17 2011
From: mcauliff <@t> umdnj.edu (Geoff McAuliffe)
Date: Tue Jun  7 08:55:45 2011
Subject: [Histonet] why no replay????
In-Reply-To: <905023.42575.qm@web112609.mail.gq1.yahoo.com>
References: <905023.42575.qm@web112609.mail.gq1.yahoo.com>
Message-ID: <4DEE2EB5.7040602@umdnj.edu>

Another possibility is that not every goblet cell is making the same 
products at the same time.

Geoff


On 6/4/2011 12:00 PM, mohamed abd el razik wrote:
> thanx to all kind replays from sgoebel, Paula Pierce and JR R.
> but i know that Alcian blue 2.5 stain most acidic mucins and pH 1 stain sulfated one. my quistion is:  i found in several serial section on intestine that 100 goblet cell react to AB ph 1 and only 50 cell reacts to AB 2.5 why?????? it should be the reverse numbers as at pH 2.5 all acidic sulfated or non sulfated are stained. in other words, ph 2.5 should stain 100 cell as ph 1 stains 100 cell at least. so i'm confused about that and replay or staining several times according to Bancroft and Farida and these was confirmed
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff@umdnj.edu
**********************************************



From sfonner <@t> labpath.com  Tue Jun  7 09:40:04 2011
From: sfonner <@t> labpath.com (Sheila Fonner)
Date: Tue Jun  7 09:43:28 2011
Subject: [Histonet] HSV I and II
Message-ID: <006a01cc2520$ca432140$5ec963c0$@com>

Good day Histonetters everywhere!

 

I was wondering if anyone could give me some brilliant advice.  We are
currently running HSVI and HSVII antibodies from Cell Marque that are still
ASR status.  My pathologist wants me to cocktail the two together.

 

Does anyone have any experience with this and could you give me any advice?
All help would be greatly appreciated.  Can I make a 50/50 mixture of the
RTU antibodies and use my longest protocol?  We are using the Ventana Ultra.

 

Thank you so much.

 

Sheila Fonner, HT (ASCP)

KDL Pathology

Knoxville, TN

 

 

From PMonfils <@t> Lifespan.org  Tue Jun  7 11:09:32 2011
From: PMonfils <@t> Lifespan.org (Monfils, Paul)
Date: Tue Jun  7 11:09:38 2011
Subject: [Histonet] Submission of tonsils to pathology
In-Reply-To: <4DE8CBA8.7400.0077.1@harthosp.org>
References: <4DE8CBA8.7400.0077.1@harthosp.org>
Message-ID: <4EBFF65383B74D49995298C4976D1D5E083E61E0@LSRIEXCH1.lsmaster.lifespan.org>

Years ago, when I was in clinical histology, this was an issue being
debated by our staff.  We had processed thousands of tonsils, and never
had a significant diagnosis.  They were leaning in the direction of
going "gross only", when guess what - a lymphoma in a routine tonsil
from a 14 year old.  So we continued microscopic evaluation on all
tonsils.  I have now been out of clinical, working in research, for
quite a few years, so the policy may have changed since then.


From SSeguin <@t> hrsrh.on.ca  Tue Jun  7 12:08:04 2011
From: SSeguin <@t> hrsrh.on.ca (Seguin, Suzanne)
Date: Tue Jun  7 12:08:11 2011
Subject: [Histonet] Re:Peloris users
Message-ID: 

Hello,
We have been using this tissue processor for 1 year now. During the last
3-6 months, we have experienced an unreasonable number of cases reported
with tissue contaminant in paraffin blocks. We use regular tissue
cassettes as well as the biopsy and micromesh style. While using this
processor I have also witnessed tissue fragment into smaller pieces
while processing. Because the Peloris uses a relatively large magnetic
stirrer, I'm wondering if this could be a cause for the contaminant. Has
anyone else experienced this? Any thoughts, suggestions?

Thanks

Sue 
Charge Technologist - Anatomic Pathology 
Sudbury Regional Hospital
41 Ramsey Lake Road, Sudbury, ON P3E 5J1
(Tel (705) 523-7100 ext. 1209
 Fax (705) 675-4776
sseguin@hrsrh.on.ca 








************************************************************************
The information contained in this e-mail and document(s) attached are for
the exclusive use of the addressee and may contain confidential, privileged
and non-disclosable information. If the recipient of this e-mail is not the
addressee, such recipient is strictly prohibited from reading, photocopying,
distributing or otherwise using this e-mail or its content in any way.
From LSetlak <@t> childrensmemorial.org  Tue Jun  7 12:45:38 2011
From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa)
Date: Tue Jun  7 12:45:45 2011
Subject: [Histonet] RE: Re:Peloris users
In-Reply-To: 
References: 
Message-ID: <7111DB39D045004C9CF29E79C71B28BC10212DFFBF@CMHEXCC01MBX.childrensmemorial.org>

Wow, I feel like I'm listening to myself speak. We have the machine for about the same amount of time as yourself and yes we have had tissue contamination. I can't say it's super bad but we've had several cases recently where as before we rarely ever had it. Unfortunately I'm not sure what causes it and I think it got blamed on the grossing person or embedder in our cases. I'm interested to see what everyone has to say about this.
Lisa

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Seguin, Suzanne
Sent: Tuesday, June 07, 2011 12:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re:Peloris users

Hello,
We have been using this tissue processor for 1 year now. During the last
3-6 months, we have experienced an unreasonable number of cases reported
with tissue contaminant in paraffin blocks. We use regular tissue
cassettes as well as the biopsy and micromesh style. While using this
processor I have also witnessed tissue fragment into smaller pieces
while processing. Because the Peloris uses a relatively large magnetic
stirrer, I'm wondering if this could be a cause for the contaminant. Has
anyone else experienced this? Any thoughts, suggestions?

Thanks

Sue 
Charge Technologist - Anatomic Pathology 
Sudbury Regional Hospital
41 Ramsey Lake Road, Sudbury, ON P3E 5J1
(Tel (705) 523-7100 ext. 1209
 Fax (705) 675-4776
sseguin@hrsrh.on.ca 








************************************************************************
The information contained in this e-mail and document(s) attached are for
the exclusive use of the addressee and may contain confidential, privileged
and non-disclosable information. If the recipient of this e-mail is not the
addressee, such recipient is strictly prohibited from reading, photocopying,
distributing or otherwise using this e-mail or its content in any way.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From TNMayer <@t> mdanderson.org  Tue Jun  7 12:54:51 2011
From: TNMayer <@t> mdanderson.org (Mayer,Toysha N)
Date: Tue Jun  7 12:54:54 2011
Subject: [Histonet] RE: Histonet Digest, Vol 91, Issue 9: Fite control block
In-Reply-To: 
References: 
Message-ID: 

Try LSU-School of Veterinary Medicine, Hansens Disease Center.  The center is a division of the CDC, and they may be able to provide blocks for controls.  They actually did a lot of research on mycobacteria.  When I worked at the Veterinary School, Hansen's would provide them as a service to the public.  The contact person used to be Joe Allen.  
Hope this helps.


Toysha N. Mayer, MBA, HT (ASCP)
Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnmayer@mdanderson.org





Does anyone know where I can get a Fite control block?

Kimberly Artim, AST, HT (ASCP)
Technical Coordinator, Anatomic Pathology
St Lukes Hospital & Health Network
801 Ostrum Street
Bethlehem, PA 18015
610-954-4832

From LSetlak <@t> childrensmemorial.org  Tue Jun  7 13:12:26 2011
From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa)
Date: Tue Jun  7 13:12:31 2011
Subject: [Histonet] question on H pylori
Message-ID: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>

I was just curious what everyone is using for standard of care regarding H =
    Pylori......is everyone doing IHC or are you doing a Giemsa?
    Thanks,
    Lisa
    Lisa M. Van Valkenberg, B.S., HT- ASCP
    Histology Manager
    2300 Children's Plaza
    Chicago, IL 60614
    773-868-8949



From micropathlabs <@t> yahoo.com  Tue Jun  7 13:17:53 2011
From: micropathlabs <@t> yahoo.com (Sheila Haas)
Date: Tue Jun  7 13:18:00 2011
Subject: [Histonet] question on H pylori
In-Reply-To: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
References: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
Message-ID: <675109.41931.qm@web161702.mail.bf1.yahoo.com>

IHC here.
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
?




________________________________
From: "Setlak, Lisa" 
To: "histonet@lists.utsouthwestern.edu" ; 
"histonet-request@lists.utsouthwestern.edu" 

Sent: Tue, June 7, 2011 2:12:26 PM
Subject: [Histonet] question on H pylori

I was just curious what everyone is using for standard of care regarding H =
? ? Pylori......is everyone doing IHC or are you doing a Giemsa?
? ? Thanks,
? ? Lisa
? ? Lisa M. Van Valkenberg, B.S., HT- ASCP
? ? Histology Manager
? ? 2300 Children's Plaza
? ? Chicago, IL 60614
? ? 773-868-8949



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From af46 <@t> buffalo.edu  Tue Jun  7 13:27:48 2011
From: af46 <@t> buffalo.edu (Annette Featherstone)
Date: Tue Jun  7 13:27:52 2011
Subject: [Histonet] paraffin block disposal in NYS
Message-ID: 

As per NYS department of Health:
Questions have also arisen regarding the appropriate disposal of organs and
tissues which have been fixed for cytological and/or histological
examination. Since the fixatives are considered to be hazardous materials,
organs and tissues discarded with these chemicals must be processed as
hazardous waste, except for blocks of tissue in paraffin or similar
embedding materials. The latter prevent the fixatives from leaching into the
environment and the chemical fixatives destroy any potential pathogens in
the tissue block. Therefore, tissue blocks can be discarded as solid waste.
 
Annette Featherstone
 
From cgill <@t> marylandgeneral.org  Tue Jun  7 13:28:40 2011
From: cgill <@t> marylandgeneral.org (Gill, Caula A.)
Date: Tue Jun  7 13:28:51 2011
Subject: [Histonet] question on H pylori
In-Reply-To: <675109.41931.qm@web161702.mail.bf1.yahoo.com>
References: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
	<675109.41931.qm@web161702.mail.bf1.yahoo.com>
Message-ID: <087A9911BBAFDE4B8151CB148586E2C23A9F76@MDGEN-EXCH1.marylandgeneral.org>

We do a Steiner

Caula Gill (HT)ASCP 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Tuesday, June 07, 2011 2:18 PM
To: Setlak, Lisa; histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu
Subject: Re: [Histonet] question on H pylori

IHC here.
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
?




________________________________
From: "Setlak, Lisa" 
To: "histonet@lists.utsouthwestern.edu" ;
"histonet-request@lists.utsouthwestern.edu" 

Sent: Tue, June 7, 2011 2:12:26 PM
Subject: [Histonet] question on H pylori

I was just curious what everyone is using for standard of care regarding H =
? ? Pylori......is everyone doing IHC or are you doing a Giemsa?
? ? Thanks,
? ? Lisa
? ? Lisa M. Van Valkenberg, B.S., HT- ASCP
? ? Histology Manager
? ? 2300 Children's Plaza
? ? Chicago, IL 60614
? ? 773-868-8949



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From lblazek <@t> digestivespecialists.com  Tue Jun  7 13:31:55 2011
From: lblazek <@t> digestivespecialists.com (Blazek, Linda)
Date: Tue Jun  7 13:31:59 2011
Subject: [Histonet] RE: question on H pylori
In-Reply-To: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
References: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBF65E9CE@IBMB7Exchange.digestivespecialists.com>

IHC here

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 396-2623
Email: lblazek@digestivespecialists.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa
Sent: Tuesday, June 07, 2011 2:12 PM
To: histonet@lists.utsouthwestern.edu; 'histonet-request@lists.utsouthwestern.edu'
Subject: [Histonet] question on H pylori

I was just curious what everyone is using for standard of care regarding H =
    Pylori......is everyone doing IHC or are you doing a Giemsa?
    Thanks,
    Lisa
    Lisa M. Van Valkenberg, B.S., HT- ASCP
    Histology Manager
    2300 Children's Plaza
    Chicago, IL 60614
    773-868-8949



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From nicole <@t> dlcjax.com  Tue Jun  7 13:35:47 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Tue Jun  7 13:36:05 2011
Subject: [Histonet] AP software
Message-ID: <4430.208.62.167.196.1307471747.squirrel@webmail.realpages.com>

Hello Histonetters,

I need help. So I have used Pathlogix software for about 4yrs and I love
it. It so super simple and have never had any problems. Well, except for
one. The cost of leasing has increase by 19% each year for no apprearent
reason. We are now paying quarterly triple what we leased it for 4yrs ago.
The owner says cost are riseing due to the economy and prices are subject
to change without notice. Really! 19%. Cause im pretty sure thats bad
business and thats how you lose customer instead of keeping them. At any
rate, I need to find a new company. I have looked into a few systems but
the cost are hugh. We are a small derm lab with one reading physician and
im the only tech. So I do not need a large system. I need to be able to
generate path reports. Accession, create reports and logs for about 3000
specimen a year. I wish I could just purchase outright but will lease if
we have to.  I havent had must luck at finding software for small labs
except Pathlogix. Love the software, not so fond of the company. Please
tell me what software is out there and If you guys like it.

Thank a million,
Nicole Tatum HT ASCP


From wbenton <@t> cua.md  Tue Jun  7 14:01:32 2011
From: wbenton <@t> cua.md (Walter Benton)
Date: Tue Jun  7 14:03:31 2011
Subject: [Histonet] AP software
In-Reply-To: <4430.208.62.167.196.1307471747.squirrel@webmail.realpages.com>
References: <4430.208.62.167.196.1307471747.squirrel@webmail.realpages.com>
Message-ID: <0B8979A204680A42B93A52B486088CD91D6EC7CEFC@CUAEXH1.GCU-MD.local>

Nicolle you may want to check into Vital Axis. It will handle the functions that you describe. They have a variety of options for pricing, which they can discuss with you. Marcus Cognetti is a good contact if you are interested. mcognetti@vitalaxis.com

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wbenton@cua.md
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum [nicole@dlcjax.com]
Sent: Tuesday, June 07, 2011 2:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AP software

Hello Histonetters,

I need help. So I have used Pathlogix software for about 4yrs and I love
it. It so super simple and have never had any problems. Well, except for
one. The cost of leasing has increase by 19% each year for no apprearent
reason. We are now paying quarterly triple what we leased it for 4yrs ago.
The owner says cost are riseing due to the economy and prices are subject
to change without notice. Really! 19%. Cause im pretty sure thats bad
business and thats how you lose customer instead of keeping them. At any
rate, I need to find a new company. I have looked into a few systems but
the cost are hugh. We are a small derm lab with one reading physician and
im the only tech. So I do not need a large system. I need to be able to
generate path reports. Accession, create reports and logs for about 3000
specimen a year. I wish I could just purchase outright but will lease if
we have to.  I havent had must luck at finding software for small labs
except Pathlogix. Love the software, not so fond of the company. Please
tell me what software is out there and If you guys like it.

Thank a million,
Nicole Tatum HT ASCP


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law.  If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy.

From mtitford <@t> aol.com  Tue Jun  7 14:16:00 2011
From: mtitford <@t> aol.com (mtitford@aol.com)
Date: Tue Jun  7 14:16:31 2011
Subject: [Histonet] Polyvinyl alcohol
Message-ID: <8CDF35E787AC92D-2684-560EF@webmail-d144.sysops.aol.com>



Candice Camille asks about polyvinyl alcohol.

Some time ago when I was in the Army Reserve I completed a correspondence course for parasitology and it discussed PVA. I still have the manual. It states: "PVA is a mixture of fixative and water soluble resin that is specifically used to fix and preserve trophozoites of intestinal amoebic organisms. " Further on it states, " PVA is primarily used for preserving fresh specimens to be shipped to central laboratories". A formula is given. I can Fax it to you if you give me a Fax number.

Regards
Michael Titford
USA - Pathology 
Mobile  AL USA


From rjbuesa <@t> yahoo.com  Tue Jun  7 14:27:07 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Jun  7 14:27:11 2011
Subject: [Histonet] question on H pylori
In-Reply-To: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
Message-ID: <365856.95700.qm@web65703.mail.ac4.yahoo.com>

Modified Steiner.
Ren? J.

--- On Tue, 6/7/11, Setlak, Lisa  wrote:


From: Setlak, Lisa 
Subject: [Histonet] question on H pylori
To: "histonet@lists.utsouthwestern.edu" , "'histonet-request@lists.utsouthwestern.edu'" 
Date: Tuesday, June 7, 2011, 2:12 PM


I was just curious what everyone is using for standard of care regarding H =
? ? Pylori......is everyone doing IHC or are you doing a Giemsa?
? ? Thanks,
? ? Lisa
? ? Lisa M. Van Valkenberg, B.S., HT- ASCP
? ? Histology Manager
? ? 2300 Children's Plaza
? ? Chicago, IL 60614
? ? 773-868-8949



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From akbitting <@t> geisinger.edu  Tue Jun  7 14:33:49 2011
From: akbitting <@t> geisinger.edu (Angela Bitting)
Date: Tue Jun  7 14:33:59 2011
Subject: [Histonet] Ventana probes
Message-ID: <4DEE44DE.2B7F.00C9.1@geisinger.edu>

I have a Pathologist asking me to try running Kappa and Lambda by CISH on bone marrow smears. Does anyone have any experience with trying that?
 
Happy strange Tuesday,
Angie


IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
From Wanda.Smith <@t> HCAhealthcare.com  Tue Jun  7 15:09:56 2011
From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com)
Date: Tue Jun  7 15:10:05 2011
Subject: [Histonet] RE: question on H pylori
In-Reply-To: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
References: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A77E4855@NADCWPMSGCMS03.hca.corpad.net>

IHC on all gastric biopsies here.

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa
Sent: Tuesday, June 07, 2011 2:12 PM
To: histonet@lists.utsouthwestern.edu; 'histonet-request@lists.utsouthwestern.edu'
Subject: [Histonet] question on H pylori

I was just curious what everyone is using for standard of care regarding H =
    Pylori......is everyone doing IHC or are you doing a Giemsa?
    Thanks,
    Lisa
    Lisa M. Van Valkenberg, B.S., HT- ASCP
    Histology Manager
    2300 Children's Plaza
    Chicago, IL 60614
    773-868-8949



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From mbrooks <@t> incytepathology.com  Tue Jun  7 15:52:52 2011
From: mbrooks <@t> incytepathology.com (Matt Brooks)
Date: Tue Jun  7 15:52:55 2011
Subject: [Histonet] RE: question on H pylori
In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA13A77E4855@NADCWPMSGCMS03.hca.corpad.net>
Message-ID: <706224670091FE47997AEF88EFADE7CA01FA5521@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM>

We perform an IHC

Matt Brooks, BS, HT (ASCP)
Histology Supervisor
InCyte Pathology
mbrooks@incytepathology.com
509-892-2744 (W)
720-253-7204 (C)

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Wanda.Smith@HCAhealthcare.com
Sent: Tuesday, June 07, 2011 1:10 PM
To: LSetlak@childrensmemorial.org; histonet@lists.utsouthwestern.edu;
histonet-request@lists.utsouthwestern.edu
Subject: [Histonet] RE: question on H pylori

IHC on all gastric biopsies here.

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or
CONFIDENTIAL information and may be read or used only by the intended
recipient. If you are not the intended recipient of the email or any of
its attachments, please be advised that you have received this email in
error and that any use, dissemination, distribution, forwarding,
printing, or copying of this email or any attached files is strictly
prohibited. If you have received this email in error, please immediately
purge it and all attachments and notify the sender by reply email or
contact the sender at the number listed.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Setlak,
Lisa
Sent: Tuesday, June 07, 2011 2:12 PM
To: histonet@lists.utsouthwestern.edu;
'histonet-request@lists.utsouthwestern.edu'
Subject: [Histonet] question on H pylori

I was just curious what everyone is using for standard of care regarding
H =
    Pylori......is everyone doing IHC or are you doing a Giemsa?
    Thanks,
    Lisa
    Lisa M. Van Valkenberg, B.S., HT- ASCP
    Histology Manager
    2300 Children's Plaza
    Chicago, IL 60614
    773-868-8949



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From foreightl <@t> gmail.com  Tue Jun  7 16:15:10 2011
From: foreightl <@t> gmail.com (Patrick Laurie)
Date: Tue Jun  7 16:15:13 2011
Subject: [Histonet] question on H pylori
In-Reply-To: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
References: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
Message-ID: 

IHC here.

On Tue, Jun 7, 2011 at 11:12 AM, Setlak, Lisa
 wrote:
> I was just curious what everyone is using for standard of care regarding H =
> ? ?Pylori......is everyone doing IHC or are you doing a Giemsa?
> ? ?Thanks,
> ? ?Lisa
> ? ?Lisa M. Van Valkenberg, B.S., HT- ASCP
> ? ?Histology Manager
> ? ?2300 Children's Plaza
> ? ?Chicago, IL 60614
> ? ?773-868-8949
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie@cellnetix.com

From nelsonrnch <@t> verizon.net  Tue Jun  7 17:15:57 2011
From: nelsonrnch <@t> verizon.net (SHANE NELSON)
Date: Tue Jun  7 17:16:00 2011
Subject: [Histonet] Nicole Tatum -AP Software
Message-ID: <479132.53598.qm@web84307.mail.re1.yahoo.com>

Nicole APEASY seems to be Great?Lab Software, very user friendly and very cost 
effective. Espicially when it comes down to?annual maintenance.
?
THANK YOU,
?
PATTI RUBEN-NELSON? H.T.(ASCP) 
PNP LABORATORY CONSULTANTS
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761 
nelsonrnch@verizon.net
From mhale <@t> carisls.com  Tue Jun  7 17:31:26 2011
From: mhale <@t> carisls.com (Hale, Meredith)
Date: Tue Jun  7 17:32:35 2011
Subject: [Histonet] PA HT Position 
Message-ID: <6F33D8418806044682A391273399860F086BEC9F@s-irv-ex301.PathologyPartners.intranet>

Great opportunity for a Histotechnician in a brand new laboratory! We
are an 8 physician Dermatology practice located in Bucks County, PA
looking for a certified HT or HTL.  Candidate must meet CLIA grossing
requirements.  The candidate will be responsible for routine histology
duties.  This is a part-time position that offers a competitive salary
and flexible hours. Interested applicants should contact Meredith Hale,
phone (214)596-2219 or e-mail mhale@carisls.com

 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 214-596-7095

mhale@carisls.com   

 

From mbrooks <@t> incytepathology.com  Tue Jun  7 18:38:52 2011
From: mbrooks <@t> incytepathology.com (Matt Brooks)
Date: Tue Jun  7 18:38:56 2011
Subject: [Histonet] Flow Cytometry
Message-ID: <706224670091FE47997AEF88EFADE7CA01FA5524@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM>

Hello All,

 

We are looking into starting performing our own Flow Cytometry.  Is
there anyone out there that is doing this and can someone recommend a
company to contact about equipment and pricing?  Your help is greatly
appreciated.

 

Thank you, 

 

Matt Brooks, BS, HT (ASCP)

Histology Supervisor

InCyte Pathology

mbrooks@incytepathology.com

509-892-2744 (W)

720-253-7204 (C)

 

From amosbrooks <@t> gmail.com  Tue Jun  7 18:50:46 2011
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Tue Jun  7 18:50:51 2011
Subject: [Histonet] Fite control block vs Block Disposal
Message-ID: 

Probably from one of these folks throwing away their blocks. A tragedy, I
tell you!

Amos


On Tue, Jun 7, 2011 at 1:00 PM,
wrote:

> Message: 9
> Date: Mon, 6 Jun 2011 15:30:33 -0400
> From: "Artim, Kimberly" 
> Subject: [Histonet] Fite control block
> To: "histonet@lists.utsouthwestern.edu"
>        
> Message-ID:
>        <9E67FDD215B226448638018A82B952BD021E7261D5BA@EXCHANGE.slhn.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone know where I can get a Fite control block?
>
> Kimberly Artim, AST, HT (ASCP)
> Technical Coordinator, Anatomic Pathology
> St Lukes Hospital & Health Network
> 801 Ostrum Street
> Bethlehem, PA 18015
> 610-954-4832
>
From marktarango <@t> gmail.com  Tue Jun  7 18:52:08 2011
From: marktarango <@t> gmail.com (Mark Tarango)
Date: Tue Jun  7 18:52:11 2011
Subject: [Histonet] Flow Cytometry
In-Reply-To: <706224670091FE47997AEF88EFADE7CA01FA5524@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM>
References: <706224670091FE47997AEF88EFADE7CA01FA5524@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM>
Message-ID: 

Hi Matt,

You might get better answers on the flow listserv.
http://www.cyto.purdue.edu/hmarchiv/index.htm

Good luck.

Mark

On Tue, Jun 7, 2011 at 4:38 PM, Matt Brooks wrote:

> Hello All,
>
>
>
> We are looking into starting performing our own Flow Cytometry.  Is
> there anyone out there that is doing this and can someone recommend a
> company to contact about equipment and pricing?  Your help is greatly
> appreciated.
>
>
>
> Thank you,
>
>
>
> Matt Brooks, BS, HT (ASCP)
>
> Histology Supervisor
>
> InCyte Pathology
>
> mbrooks@incytepathology.com
>
> 509-892-2744 (W)
>
> 720-253-7204 (C)
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From amosbrooks <@t> gmail.com  Tue Jun  7 19:06:15 2011
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Tue Jun  7 19:14:01 2011
Subject: [Histonet] Re: Histonet Digest, Vol 91, Issue 9
In-Reply-To: <4dee593a.053a650a.1047.ffffa01eSMTPIN_ADDED@mx.google.com>
References: <4dee593a.053a650a.1047.ffffa01eSMTPIN_ADDED@mx.google.com>
Message-ID: 

Hi,
    You could try Newcomer Supply.
http://www.newcomersupply.com/products-positive-qc-histopathology.php They
have an AFB control that they say has "Mycobacterium sp. Tuberculosis
positive and negative tissue in same section". That should work.
Amos

On Tue, Jun 7, 2011 at 1:00 PM,
wrote:

> Message: 9
> Date: Mon, 6 Jun 2011 15:30:33 -0400
> From: "Artim, Kimberly" 
> Subject: [Histonet] Fite control block
> To: "histonet@lists.utsouthwestern.edu"
>        
> Message-ID:
>        <9E67FDD215B226448638018A82B952BD021E7261D5BA@EXCHANGE.slhn.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone know where I can get a Fite control block?
>
> Kimberly Artim, AST, HT (ASCP)
> Technical Coordinator, Anatomic Pathology
> St Lukes Hospital & Health Network
> 801 Ostrum Street
> Bethlehem, PA 18015
> 610-954-4832
>
From j.brinker <@t> att.net  Tue Jun  7 20:23:39 2011
From: j.brinker <@t> att.net (Jean Brinker)
Date: Tue Jun  7 20:23:37 2011
Subject: [Histonet] AP software
In-Reply-To: <4430.208.62.167.196.1307471747.squirrel@webmail.realpages.com>
References: <4430.208.62.167.196.1307471747.squirrel@webmail.realpages.com>
Message-ID: <1041701992-1307496213-cardhu_decombobulator_blackberry.rim.net-128648223-@b14.c13.bise6.blackberry>

Nicole, we've been pretty happy with APEasy as well. 
Sent from my BlackBerry

-----Original Message-----
From: "Nicole Tatum" 
Sender: histonet-bounces@lists.utsouthwestern.edu
Date: Tue, 7 Jun 2011 14:35:47 
To: 
Subject: [Histonet] AP software

Hello Histonetters,

I need help. So I have used Pathlogix software for about 4yrs and I love
it. It so super simple and have never had any problems. Well, except for
one. The cost of leasing has increase by 19% each year for no apprearent
reason. We are now paying quarterly triple what we leased it for 4yrs ago.
The owner says cost are riseing due to the economy and prices are subject
to change without notice. Really! 19%. Cause im pretty sure thats bad
business and thats how you lose customer instead of keeping them. At any
rate, I need to find a new company. I have looked into a few systems but
the cost are hugh. We are a small derm lab with one reading physician and
im the only tech. So I do not need a large system. I need to be able to
generate path reports. Accession, create reports and logs for about 3000
specimen a year. I wish I could just purchase outright but will lease if
we have to.  I havent had must luck at finding software for small labs
except Pathlogix. Love the software, not so fond of the company. Please
tell me what software is out there and If you guys like it.

Thank a million,
Nicole Tatum HT ASCP


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From rsrichmond <@t> gmail.com  Tue Jun  7 21:07:18 2011
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Tue Jun  7 21:07:22 2011
Subject: [Histonet] Re: Fite control block
Message-ID: 

Kimberly Artim, AST, HT (ASCP), Technical Coordinator, Anatomic
Pathology, St Lukes Hospital & Health Network, Bethlehem, Pennsylvania
asks about a Fite control block.

Do you mean a block of tissue with Mycobacterium leprae?

It's preferable indeed to have lepra bacilli rather than tubercle
bacilli in the control, since the two organisms stain somewhat
differently. Obviously human tissue is extremely rare. Does anyone use
leprous armadillo (Dasypus novemcinctus) tissue as a control? Does
anyone know how to get it?

Bob Richmond
Samurai Pathologist
Knoxville TN

From GDawson <@t> dynacaremilwaukee.com  Wed Jun  8 07:33:57 2011
From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen)
Date: Wed Jun  8 07:34:04 2011
Subject: [Histonet] RE: question on H pylori
In-Reply-To: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
References: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
Message-ID: 

Lisa,

We use both IHC and Warthin Starry here, depending on the Pathologist's preference.

Glen Dawson  BS, HT(ASCP) & QIHC
IHC Manager
Milwaukee, WI

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa
Sent: Tuesday, June 07, 2011 1:12 PM
To: histonet@lists.utsouthwestern.edu; 'histonet-request@lists.utsouthwestern.edu'
Subject: [Histonet] question on H pylori

I was just curious what everyone is using for standard of care regarding H =
    Pylori......is everyone doing IHC or are you doing a Giemsa?
    Thanks,
    Lisa
    Lisa M. Van Valkenberg, B.S., HT- ASCP
    Histology Manager
    2300 Children's Plaza
    Chicago, IL 60614
    773-868-8949



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From LSebree <@t> uwhealth.org  Wed Jun  8 07:39:49 2011
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Wed Jun  8 07:40:29 2011
Subject: [Histonet] HSV I and II
In-Reply-To: <006a01cc2520$ca432140$5ec963c0$@com>
References: <006a01cc2520$ca432140$5ec963c0$@com>
Message-ID: 

Sheila,

We use CM's HSV I (361A) and HSV II (362A) and cocktail them for use on
VMS's BenchMark and XTs.   I use them  cocktailed to achieve a 1:100
dilution of both with mild CC1 @ 42 degrees C for 32 minutes with AB
block and Hem II/8 minutes.

Hope this helps,

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila
Fonner
Sent: Tuesday, June 07, 2011 9:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HSV I and II

Good day Histonetters everywhere!

 

I was wondering if anyone could give me some brilliant advice.  We are
currently running HSVI and HSVII antibodies from Cell Marque that are
still
ASR status.  My pathologist wants me to cocktail the two together.

 

Does anyone have any experience with this and could you give me any
advice?
All help would be greatly appreciated.  Can I make a 50/50 mixture of
the
RTU antibodies and use my longest protocol?  We are using the Ventana
Ultra.

 

Thank you so much.

 

Sheila Fonner, HT (ASCP)

KDL Pathology

Knoxville, TN

 

 

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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From etambutte <@t> centrescientifique.mc  Wed Jun  8 07:46:15 2011
From: etambutte <@t> centrescientifique.mc (Eric Tambutte)
Date: Wed Jun  8 07:46:20 2011
Subject: [Histonet] Re: Histonet Digest, Vol 91, Issue 10
Message-ID: <1053324743@s15272523.onlinehome-server.info>

Merci pour votre message.
Je suis actuellement absent du laboratoire.
Je repondrai a votre message a mon retour a partir du 20 juin 2011.


Thank you for your email.
I am currently out of the lab.
I will reply to your message after the 20th of june 2011.

From rsrichmond <@t> gmail.com  Wed Jun  8 08:25:11 2011
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Wed Jun  8 08:25:17 2011
Subject: [Histonet] Re: question on H pylori
Message-ID: 

On the question of staining Helicobacter pylori:

There is no "standard of care" (remember that "standard of care" is a
term of art and should not be used casually) for staining
Helicobacter. As far as I know, nobody has ever done a study comparing
the efficacy of the various staining methods.

Blue stains (usually called Giemsa) may still be most commonly used.
The blue component of Diff-Quik (Diff-Quik II - any generic equivalent
will do) suffices and is much simpler to do. Some labs that do their
own preparation make a suitably buffered solution of toluidine blue.

Many labs have gone to immunostaining. This decision needs to be made
partly on reimbursement considerations, particularly if you're going
to outsource the staining and have your pathologist read the slides. -
Worthwhile knowing that the rare (I've seen it once) Helicobacter
heilmanii immunostains as well as does Helicobacter pylori.

I haven't seen silver stains (Steiner, Warthin-Starry) in use for
quite a few years. They are so difficult to do that I can't imagine
anyone still using them.

My personal preference is a blue stain if I have only one slide a day
to read, but IHC if I have ten - IHC can be read much faster with much
less magnification.

An interesting new development for IHC is virtual slide technology -
Genzyme (I have no connection with Genzyme) does the IHC and uploads a
virtual slide, which the pathologist interprets. One associate of mine
finds this highly satisfactory - I find it slow and cumbersome and I'm
never sure I've seen the whole slide. (I have some vision issues with
it - remember I'm 72 years old and quite nearsighted.)

Bob Richmond
Samurai Pathologist
Knoxville TN

From mike <@t> triagestaff.com  Wed Jun  8 08:37:20 2011
From: mike <@t> triagestaff.com (Mike Comstock)
Date: Wed Jun  8 08:37:04 2011
Subject: [Histonet] Contract Histology Positions
Message-ID: <279084206369399@send.pltechnology.com>


Greetings:

My name is Mike Comstock and I'm with a group out of Omaha called Triage Staffing, Inc.  Our company specializes in working with laboratories nationwide who are in need of temporary Histology professionals.  I wanted to post my contact information on Histonet in the hopes of reaching some of you who might currently be available for contract work or who might be considering it sometime down the road.

Our clients include hospitals and reference laboratories who have temporary openings for a variety of reasons - vacation coverages, medical leaves, and often just to provide relief to their full-time staff.

Feel free to contact me directly if you have an interest in hearing more about our company.  Email - mike@triagestaff.com or call 800.259.9897 x204  Our website is www.triagestaff.com

I'm new to Histonet but if it's appropriate I may be posting specific jobs from time to time with regard to location, shift, length of assignment etc.

Thanks for taking the time to read my email and I look forward to hearing from some of you!  



Mike Comstock
Account Manager, Laboratory Division
Triage Staffing, Inc
P. 800.259.9897 ext 204
F. 800.259.0287
www.TriageStaff.com

(The following links were included with this email:)
mailto:mike@triagestaff.com

http://www.triagestaff.com



(The following links were included with this email:)
mailto:mike@triagestaff.com

http://www.triagestaff.com


From NLEMKE1 <@t> hfhs.org  Wed Jun  8 09:16:06 2011
From: NLEMKE1 <@t> hfhs.org (Lemke, Nancy)
Date: Wed Jun  8 09:16:24 2011
Subject: [Histonet] frozen sample cassettes
Message-ID: 

Greetings Histofolk!
I have run out of a cassette that I use for storage of frozen rodent brains.  It is about the general dimensions of a routine processing cassette but has a screw cap that seals the sample into a round storage area which has no openings other than the screw off lid..  There is an angled edge for labeling and the plastic almost looks like Nalgene opaque plastic.  I use these for storage in -80 and they are perfect but I have not been able to find them again after many, many years.  I believe they were from a start-up company years ago so they might not be available any more.
Any thoughts?
Thanks,
Nancy W Lemke BA, HT(ASCP)HTL
Research Coordinator
Hermelin Brain Tumor Center
Neurosurgery Research
Henry Ford Hospital
Detroit, MI
P:(313) 916-8648
F:(313) 916-9855

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From kiran_g <@t> sbcglobal.net  Wed Jun  8 09:25:03 2011
From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal)
Date: Wed Jun  8 09:25:20 2011
Subject: [Histonet] Dako Autostainer: Need as a Loaner for a year
Message-ID: <87667.89120.qm@web180107.mail.gq1.yahoo.com>

Please help me find out if we can get a loaner Dako Autostainer for a year.
?
We will be doing a project and don't want to buy a new stainer due to budget restrains.
?
Any help is appericiated.
?
I also need Dako contact info for the Bay area( CA).
?
thank you so much!
?
- Kiranjit
From brett_connolly <@t> merck.com  Wed Jun  8 09:39:16 2011
From: brett_connolly <@t> merck.com (Connolly, Brett M)
Date: Wed Jun  8 09:39:22 2011
Subject: [Histonet] MMP-3 IHC on rat tissue
Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D7EA0342@usctmx1176.merck.com>

Histonetters,

Has anyone performed MMP-3 IHC on FFPE rat tissue??  I see Epitomics has
a RabMAB, but rat cross reactivity is only confirmed by Western. Would
like some suggestions from any MMP-3 users.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly@merck.com
T- 215-652-2501
F- 215-993-6803




Notice:  This e-mail message, together with any attachments, contains
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please notify us immediately by reply e-mail and then delete it from 
your system.
From Allison_Scott <@t> hchd.tmc.edu  Wed Jun  8 10:12:10 2011
From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D)
Date: Wed Jun  8 10:12:14 2011
Subject: [Histonet] AP Benchmarks
Message-ID: <1872B4A455B7974391609AD8034C79FC026DFDFA@LBEXCH01.hchd.local>

Hello to all in histoland.  Does anyone know a website where you can get
AP benchmark information.  I am in need of information to justify a new
position in histology.  Any help will be greatly appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
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From rjbuesa <@t> yahoo.com  Wed Jun  8 10:15:38 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Jun  8 10:15:42 2011
Subject: [Histonet] AP Benchmarks
In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFDFA@LBEXCH01.hchd.local>
Message-ID: <23028.34816.qm@web65713.mail.ac4.yahoo.com>

Under separate cover I am sending all the benchmark information you need.
Ren? J.

--- On Wed, 6/8/11, Scott, Allison D  wrote:


From: Scott, Allison D 
Subject: [Histonet] AP Benchmarks
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, June 8, 2011, 11:12 AM


Hello to all in histoland.? Does anyone know a website where you can get
AP benchmark information.? I am in need of information to justify a new
position in histology.? Any help will be greatly appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
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From margaretschefdore <@t> yahoo.com  Wed Jun  8 11:24:12 2011
From: margaretschefdore <@t> yahoo.com (Margaret Schefdore)
Date: Wed Jun  8 11:24:16 2011
Subject: [Histonet] unsubscibe
Message-ID: <878738.60800.qm@web112320.mail.gq1.yahoo.com>

please tell me how to unsubscibe the histonet@lists.utsouthwestern.edu. my mail 
box gets too full.
From margaretschefdore <@t> yahoo.com  Wed Jun  8 11:24:25 2011
From: margaretschefdore <@t> yahoo.com (Margaret Schefdore)
Date: Wed Jun  8 11:24:31 2011
Subject: [Histonet] (no subject)
Message-ID: <510044.63710.qm@web112308.mail.gq1.yahoo.com>


From JWeems <@t> sjha.org  Wed Jun  8 11:28:29 2011
From: JWeems <@t> sjha.org (Weems, Joyce)
Date: Wed Jun  8 11:28:35 2011
Subject: [Histonet] unsubscibe
In-Reply-To: <878738.60800.qm@web112320.mail.gq1.yahoo.com>
References: <878738.60800.qm@web112320.mail.gq1.yahoo.com>
Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408210682BB@CHEXCMS10.one.ads.che.org>

Go to the website   http://lists.utsouthwestern.edu/mailman/listinfo/histonet  and follow the instructions... You need to do it yourself..  


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaret Schefdore
Sent: Wednesday, June 08, 2011 12:24
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] unsubscibe

please tell me how to unsubscibe the histonet@lists.utsouthwestern.edu. my mail box gets too full.
_______________________________________________
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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From rmweber113 <@t> comcast.net  Wed Jun  8 12:00:01 2011
From: rmweber113 <@t> comcast.net (rmweber113@comcast.net)
Date: Wed Jun  8 12:00:03 2011
Subject: [Histonet] Manger position in Torrance CA
In-Reply-To: <1214107007.1374349.1307552307430.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net>
Message-ID: <1445510373.1374426.1307552401036.JavaMail.root@sz0046a.westchester.pa.mail.comcast.net>



I have a position for a histologist looking to work about 30hours a week in a brand new in office physician?laboratory in Torrance Ca.? Hours are flexible.? Laboratory is scheduled to open in August.? ?Candidate must? be able to work independently, be ASCP certified and have CLIA grossing credentials. 

Interested candidates can fax resume at 267 722-8304. 



Marilynn Weber H.T.( ASCP ) QIHC 
Coastal Pathology Consulting Services LLC 
732 814-1170 
fax 267 722-8308 
From brett_connolly <@t> merck.com  Wed Jun  8 12:15:06 2011
From: brett_connolly <@t> merck.com (Connolly, Brett M)
Date: Wed Jun  8 12:15:15 2011
Subject: [Histonet] Toluidine blue in decaled bone
Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D7EA03EA@usctmx1176.merck.com>

We may need to do toluidine blue staining to assess cartilage changes on
FFPE EDTA decalcified rat joints. We don't have experience w/ T blue and
I have read the EDTA will extract proteoglycans.

Help?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly@merck.com
T- 215-652-2501
F- 215-993-6803



 
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
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From Rcartun <@t> harthosp.org  Wed Jun  8 12:38:11 2011
From: Rcartun <@t> harthosp.org (Richard Cartun)
Date: Wed Jun  8 12:38:18 2011
Subject: [Histonet] question on H pylori
In-Reply-To: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
References: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
Message-ID: <4DEF7B43.7400.0077.1@harthosp.org>

We use IHC on cases that show the appropriate inflammatory background.  Please note that histochemical stains may not identify intracellular H. pylori.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Setlak, Lisa"  6/7/2011 2:12 PM >>>
I was just curious what everyone is using for standard of care regarding H =
    Pylori......is everyone doing IHC or are you doing a Giemsa?
    Thanks,
    Lisa
    Lisa M. Van Valkenberg, B.S., HT- ASCP
    Histology Manager
    2300 Children's Plaza
    Chicago, IL 60614
    773-868-8949



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From LINDA.MARGRAF <@t> childrens.com  Wed Jun  8 13:00:39 2011
From: LINDA.MARGRAF <@t> childrens.com (Linda Margraf)
Date: Wed Jun  8 13:00:58 2011
Subject: [Histonet] unsubscibe
In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E16408210682BB@CHEXCMS10.one.ads.che.org>
References: <878738.60800.qm@web112320.mail.gq1.yahoo.com>
	<92AD9B20A6C38C4587A9FEBE3A30E16408210682BB@CHEXCMS10.one.ads.che.org>
Message-ID: <683621D7852C2F488898D0AC7F164A98882DDAE0@CMCPBEXMAIL02.Childrens.med>

You can also consider switching to the digest version of the emails where you get all the Histonet emails from each day batched into a single message delivered every night. You can switch to digest mode at the same site referred to below, where you would go to unsubscribe.
Linda M
Histonet administrator 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Wednesday, June 08, 2011 11:28 AM
To: Margaret Schefdore; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] unsubscibe

Go to the website   http://lists.utsouthwestern.edu/mailman/listinfo/histonet  and follow the instructions... You need to do it yourself..  


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaret Schefdore
Sent: Wednesday, June 08, 2011 12:24
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] unsubscibe

please tell me how to unsubscibe the histonet@lists.utsouthwestern.edu. my mail box gets too full.
_______________________________________________
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From MSHERWOOD <@t> PARTNERS.ORG  Wed Jun  8 13:14:08 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Wed Jun  8 13:14:13 2011
Subject: [Histonet] Pre-made paraformaldehyde question
In-Reply-To: 
References: 
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB57D6@PHSXMB30.partners.org>

I don't believe that anyone responded to your inquiry.  I used to make up a
Karnovsky's fixative (for TEM) (paraformaldehyde/glutaraldehyde) using the
powder form of paraformaldehyde.  It is highly toxic.  No one that I know of
does this anymore (or shouldn't).  Now I buy the 16% solution of
paraformaldehyde (comes in sealed 10ml ampules).  I also buy my glutaraldehyde
solution that way.  Then I make whatever dilution I need.  Much safer and
simpler this way.

Peggy 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suhyoung Jeong
Sent: Monday, June 06, 2011 8:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pre-made paraformaldehyde question

Dear Histo people,

In my old lab, I have been taught that 4% PFA has to be always made fresh
(maybe a day before max). Here in my new lab, I find that people use 32%
pre-made PFA from EMS (without expiry date) and only dilute it on the day of
use.

Does anyone have experience (or thoughts) with this issue?

Thank you in advance

All the best, Suh
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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From NLEMKE1 <@t> hfhs.org  Wed Jun  8 13:22:22 2011
From: NLEMKE1 <@t> hfhs.org (Lemke, Nancy)
Date: Wed Jun  8 13:22:39 2011
Subject: FW: [Histonet] frozen sample cassettes
In-Reply-To: <1104059394-1307553847-cardhu_decombobulator_blackberry.rim.net-1368289752-@b1.c8.bise6.blackberry>
References: ,
	<1104059394-1307553847-cardhu_decombobulator_blackberry.rim.net-1368289752-@b1.c8.bise6.blackberry>
Message-ID: <4D2868D1-391A-4570-9ED3-8BCAF55DE0DE@mimectl>

Here is information for these excellent storage cassettes.  Thanks Eileen for the response!

Nancy W Lemke BA, HT(ASCP)HTL
Research Coordinator
Hermelin Brain Tumor Center
Neurosurgery Research
Henry Ford Hospital
Detroit, MI
P:(313) 916-8648
F:(313) 916-9855
________________________________
From: elonergan@metrocast.net [elonergan@metrocast.net]
Sent: Wednesday, June 08, 2011 1:27 PM
To: Lemke, Nancy
Subject: Re: [Histonet] frozen sample cassettes

Nancy
I helped develop this unit. I'll get the new company name. They used to have a website, try it. I think its just cryosette.com. Or you can google it. I'll still locate the current vendor for you. Eileen lonergan
Sent via BlackBerry by AT&T

-----Original Message-----
From: "Lemke, Nancy" 
Sender: histonet-bounces@lists.utsouthwestern.edu
Date: Wed, 8 Jun 2011 10:16:06
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] frozen sample cassettes

Greetings Histofolk!
I have run out of a cassette that I use for storage of frozen rodent brains.  It is about the general dimensions of a routine processing cassette but has a screw cap that seals the sample into a round storage area which has no openings other than the screw off lid..  There is an angled edge for labeling and the plastic almost looks like Nalgene opaque plastic.  I use these for storage in -80 and they are perfect but I have not been able to find them again after many, many years.  I believe they were from a start-up company years ago so they might not be available any more.
Any thoughts?
Thanks,
Nancy W Lemke BA, HT(ASCP)HTL
Research Coordinator
Hermelin Brain Tumor Center
Neurosurgery Research
Henry Ford Hospital
Detroit, MI
P:(313) 916-8648
F:(313) 916-9855

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From MSHERWOOD <@t> PARTNERS.ORG  Wed Jun  8 13:25:51 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Wed Jun  8 13:25:55 2011
Subject: [Histonet] Pre-made paraformaldehyde question
In-Reply-To: 
References: 
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB57D7@PHSXMB30.partners.org>

I failed to mention that I make up my Karnovsky's and freeze aliquots.  I have
never had a problem. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suhyoung Jeong
Sent: Monday, June 06, 2011 8:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pre-made paraformaldehyde question

Dear Histo people,

In my old lab, I have been taught that 4% PFA has to be always made fresh
(maybe a day before max). Here in my new lab, I find that people use 32%
pre-made PFA from EMS (without expiry date) and only dilute it on the day of
use.

Does anyone have experience (or thoughts) with this issue?

Thank you in advance

All the best, Suh
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


From CPhipps <@t> cblpath.com  Wed Jun  8 15:37:54 2011
From: CPhipps <@t> cblpath.com (Carlene K. Phipps)
Date: Wed Jun  8 15:39:18 2011
Subject: [Histonet] RE: Histonet Digest, Vol 91, Issue 10
In-Reply-To: 
References: 
Message-ID: <0EBA2D55BAF71C4984EF84CC969C77CA08E3B2DD@OcalaExchange.cblpath.local>

IHC 

Carlene 
Email: cphipps@cblpath.com 
 
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Wednesday, June 08, 2011 8:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 91, Issue 10

Send Histonet mailing list submissions to
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To subscribe or unsubscribe via the World Wide Web, visit
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Re:Peloris users (Seguin, Suzanne)
   2. RE: Re:Peloris users (Setlak, Lisa)
   3. RE: Histonet Digest, Vol 91, Issue 9: Fite control block
      (Mayer,Toysha N)
   4. question on H pylori (Setlak, Lisa)
   5. Re: question on H pylori (Sheila Haas)
   6. paraffin block disposal in NYS (Annette Featherstone)
   7. RE: question on H pylori (Gill, Caula A.)
   8. RE: question on H pylori (Blazek, Linda)
   9. AP software (Nicole Tatum)
  10. RE: AP software (Walter Benton)
  11. Polyvinyl alcohol (mtitford@aol.com)
  12. Re: question on H pylori (Rene J Buesa)
  13. Ventana probes (Angela Bitting)
  14. RE: question on H pylori (Wanda.Smith@HCAhealthcare.com)
  15. RE: RE: question on H pylori (Matt Brooks)
  16. Re: question on H pylori (Patrick Laurie)
  17. Nicole Tatum -AP Software (SHANE NELSON)
  18. PA HT Position  (Hale, Meredith)
  19. Flow Cytometry (Matt Brooks)
  20. Fite control block vs Block Disposal (Amos Brooks)
  21. Re: Flow Cytometry (Mark Tarango)
  22. Re: Histonet Digest, Vol 91, Issue 9 (Amos Brooks)
  23. Re: AP software (Jean Brinker)
  24. Re: Fite control block (Robert Richmond)
  25. RE: question on H pylori (Dawson, Glen)


----------------------------------------------------------------------

Message: 1
Date: Tue, 7 Jun 2011 13:08:04 -0400
From: "Seguin, Suzanne" 
Subject: [Histonet] Re:Peloris users
To: 
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

Hello,
We have been using this tissue processor for 1 year now. During the last
3-6 months, we have experienced an unreasonable number of cases reported
with tissue contaminant in paraffin blocks. We use regular tissue
cassettes as well as the biopsy and micromesh style. While using this
processor I have also witnessed tissue fragment into smaller pieces
while processing. Because the Peloris uses a relatively large magnetic
stirrer, I'm wondering if this could be a cause for the contaminant. Has
anyone else experienced this? Any thoughts, suggestions?

Thanks

Sue 
Charge Technologist - Anatomic Pathology 
Sudbury Regional Hospital
41 Ramsey Lake Road, Sudbury, ON P3E 5J1
(Tel (705) 523-7100 ext. 1209
 Fax (705) 675-4776
sseguin@hrsrh.on.ca 








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The information contained in this e-mail and document(s) attached are for
the exclusive use of the addressee and may contain confidential, privileged
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------------------------------

Message: 2
Date: Tue, 7 Jun 2011 12:45:38 -0500
From: "Setlak, Lisa" 
Subject: [Histonet] RE: Re:Peloris users
To: "'Seguin, Suzanne'" ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<7111DB39D045004C9CF29E79C71B28BC10212DFFBF@CMHEXCC01MBX.childrensmemorial.org>
	
Content-Type: text/plain; charset="us-ascii"

Wow, I feel like I'm listening to myself speak. We have the machine for about the same amount of time as yourself and yes we have had tissue contamination. I can't say it's super bad but we've had several cases recently where as before we rarely ever had it. Unfortunately I'm not sure what causes it and I think it got blamed on the grossing person or embedder in our cases. I'm interested to see what everyone has to say about this.
Lisa

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Seguin, Suzanne
Sent: Tuesday, June 07, 2011 12:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re:Peloris users

Hello,
We have been using this tissue processor for 1 year now. During the last
3-6 months, we have experienced an unreasonable number of cases reported
with tissue contaminant in paraffin blocks. We use regular tissue
cassettes as well as the biopsy and micromesh style. While using this
processor I have also witnessed tissue fragment into smaller pieces
while processing. Because the Peloris uses a relatively large magnetic
stirrer, I'm wondering if this could be a cause for the contaminant. Has
anyone else experienced this? Any thoughts, suggestions?

Thanks

Sue 
Charge Technologist - Anatomic Pathology 
Sudbury Regional Hospital
41 Ramsey Lake Road, Sudbury, ON P3E 5J1
(Tel (705) 523-7100 ext. 1209
 Fax (705) 675-4776
sseguin@hrsrh.on.ca 








************************************************************************
The information contained in this e-mail and document(s) attached are for
the exclusive use of the addressee and may contain confidential, privileged
and non-disclosable information. If the recipient of this e-mail is not the
addressee, such recipient is strictly prohibited from reading, photocopying,
distributing or otherwise using this e-mail or its content in any way.
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------------------------------

Message: 3
Date: Tue, 7 Jun 2011 12:54:51 -0500
From: "Mayer,Toysha N" 
Subject: [Histonet] RE: Histonet Digest, Vol 91, Issue 9: Fite control
	block
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID:
	
	
Content-Type: text/plain; charset="us-ascii"

Try LSU-School of Veterinary Medicine, Hansens Disease Center.  The center is a division of the CDC, and they may be able to provide blocks for controls.  They actually did a lot of research on mycobacteria.  When I worked at the Veterinary School, Hansen's would provide them as a service to the public.  The contact person used to be Joe Allen.  
Hope this helps.


Toysha N. Mayer, MBA, HT (ASCP)
Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnmayer@mdanderson.org





Does anyone know where I can get a Fite control block?

Kimberly Artim, AST, HT (ASCP)
Technical Coordinator, Anatomic Pathology
St Lukes Hospital & Health Network
801 Ostrum Street
Bethlehem, PA 18015
610-954-4832



------------------------------

Message: 4
Date: Tue, 7 Jun 2011 13:12:26 -0500
From: "Setlak, Lisa" 
Subject: [Histonet] question on H pylori
To: "histonet@lists.utsouthwestern.edu"
	,
	"'histonet-request@lists.utsouthwestern.edu'"
	
Message-ID:
	<7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org>
	
Content-Type: text/plain; charset="us-ascii"

I was just curious what everyone is using for standard of care regarding H =
    Pylori......is everyone doing IHC or are you doing a Giemsa?
    Thanks,
    Lisa
    Lisa M. Van Valkenberg, B.S., HT- ASCP
    Histology Manager
    2300 Children's Plaza
    Chicago, IL 60614
    773-868-8949





------------------------------

Message: 5
Date: Tue, 7 Jun 2011 11:17:53 -0700 (PDT)
From: Sheila Haas 
Subject: Re: [Histonet] question on H pylori
To: "Setlak, Lisa" ,
	"histonet@lists.utsouthwestern.edu"
	,
	"histonet-request@lists.utsouthwestern.edu"
	
Message-ID: <675109.41931.qm@web161702.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

IHC here.
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
?




________________________________
From: "Setlak, Lisa" 
To: "histonet@lists.utsouthwestern.edu" ; 
"histonet-request@lists.utsouthwestern.edu" 

Sent: Tue, June 7, 2011 2:12:26 PM
Subject: [Histonet] question on H pylori

I was just curious what everyone is using for standard of care regarding H =
? ? Pylori......is everyone doing IHC or are you doing a Giemsa?
? ? Thanks,
? ? Lisa
? ? Lisa M. Van Valkenberg, B.S., HT- ASCP
? ? Histology Manager
? ? 2300 Children's Plaza
? ? Chicago, IL 60614
? ? 773-868-8949



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 6
Date: Tue, 7 Jun 2011 14:27:48 -0400
From: "Annette Featherstone" 
Subject: [Histonet] paraffin block disposal in NYS
To: 
Message-ID:
	
	
Content-Type: text/plain;	charset="us-ascii"

As per NYS department of Health:
Questions have also arisen regarding the appropriate disposal of organs and
tissues which have been fixed for cytological and/or histological
examination. Since the fixatives are considered to be hazardous materials,
organs and tissues discarded with these chemicals must be processed as
hazardous waste, except for blocks of tissue in paraffin or similar
embedding materials. The latter prevent the fixatives from leaching into the
environment and the chemical fixatives destroy any potential pathogens in
the tissue block. Therefore, tissue blocks can be discarded as solid waste.
 
Annette Featherstone
 


------------------------------

Message: 7
Date: Tue, 7 Jun 2011 14:28:40 -0400
From: "Gill, Caula A." 
Subject: RE: [Histonet] question on H pylori
To: "Sheila Haas" ,	"Setlak, Lisa"
	,	,
	
Message-ID:
	<087A9911BBAFDE4B8151CB148586E2C23A9F76@MDGEN-EXCH1.marylandgeneral.org>
	
Content-Type: text/plain;	charset="iso-8859-1"

We do a Steiner

Caula Gill (HT)ASCP 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Tuesday, June 07, 2011 2:18 PM
To: Setlak, Lisa; histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu
Subject: Re: [Histonet] question on H pylori

IHC here.
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
?




________________________________
From: "Setlak, Lisa" 
To: "histonet@lists.utsouthwestern.edu" ;
"histonet-request@lists.utsouthwestern.edu" 

Sent: Tue, June 7, 2011 2:12:26 PM
Subject: [Histonet] question on H pylori

I was just curious what everyone is using for standard of care regarding H =
? ? Pylori......is everyone doing IHC or are you doing a Giemsa?
? ? Thanks,
? ? Lisa
? ? Lisa M. Van Valkenberg, B.S., HT- ASCP
? ? Histology Manager
? ? 2300 Children's Plaza
? ? Chicago, IL 60614
? ? 773-868-8949



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 8
Date: Tue, 7 Jun 2011 14:31:55 -0400
From: "Blazek, Linda" 
Subject: [Histonet] RE: question on H pylori
To: "'Setlak, Lisa'" ,
	"histonet@lists.utsouthwestern.edu"
	,
	"'histonet-request@lists.utsouthwestern.edu'"
	
Message-ID:
	<5A2BD13465E061429D6455C8D6B40E390EBF65E9CE@IBMB7Exchange.digestivespecialists.com>
	
Content-Type: text/plain; charset="us-ascii"

IHC here

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 396-2623
Email: lblazek@digestivespecialists.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa
Sent: Tuesday, June 07, 2011 2:12 PM
To: histonet@lists.utsouthwestern.edu; 'histonet-request@lists.utsouthwestern.edu'
Subject: [Histonet] question on H pylori

I was just curious what everyone is using for standard of care regarding H =
    Pylori......is everyone doing IHC or are you doing a Giemsa?
    Thanks,
    Lisa
    Lisa M. Van Valkenberg, B.S., HT- ASCP
    Histology Manager
    2300 Children's Plaza
    Chicago, IL 60614
    773-868-8949



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 9
Date: Tue, 7 Jun 2011 14:35:47 -0400 (EDT)
From: "Nicole Tatum" 
Subject: [Histonet] AP software
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<4430.208.62.167.196.1307471747.squirrel@webmail.realpages.com>
Content-Type: text/plain;charset=iso-8859-1

Hello Histonetters,

I need help. So I have used Pathlogix software for about 4yrs and I love
it. It so super simple and have never had any problems. Well, except for
one. The cost of leasing has increase by 19% each year for no apprearent
reason. We are now paying quarterly triple what we leased it for 4yrs ago.
The owner says cost are riseing due to the economy and prices are subject
to change without notice. Really! 19%. Cause im pretty sure thats bad
business and thats how you lose customer instead of keeping them. At any
rate, I need to find a new company. I have looked into a few systems but
the cost are hugh. We are a small derm lab with one reading physician and
im the only tech. So I do not need a large system. I need to be able to
generate path reports. Accession, create reports and logs for about 3000
specimen a year. I wish I could just purchase outright but will lease if
we have to.  I havent had must luck at finding software for small labs
except Pathlogix. Love the software, not so fond of the company. Please
tell me what software is out there and If you guys like it.

Thank a million,
Nicole Tatum HT ASCP




------------------------------

Message: 10
Date: Tue, 7 Jun 2011 15:01:32 -0400
From: Walter Benton 
Subject: RE: [Histonet] AP software
To: Nicole Tatum ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	<0B8979A204680A42B93A52B486088CD91D6EC7CEFC@CUAEXH1.GCU-MD.local>
Content-Type: text/plain; charset="us-ascii"

Nicolle you may want to check into Vital Axis. It will handle the functions that you describe. They have a variety of options for pricing, which they can discuss with you. Marcus Cognetti is a good contact if you are interested. mcognetti@vitalaxis.com

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wbenton@cua.md
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum [nicole@dlcjax.com]
Sent: Tuesday, June 07, 2011 2:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AP software

Hello Histonetters,

I need help. So I have used Pathlogix software for about 4yrs and I love
it. It so super simple and have never had any problems. Well, except for
one. The cost of leasing has increase by 19% each year for no apprearent
reason. We are now paying quarterly triple what we leased it for 4yrs ago.
The owner says cost are riseing due to the economy and prices are subject
to change without notice. Really! 19%. Cause im pretty sure thats bad
business and thats how you lose customer instead of keeping them. At any
rate, I need to find a new company. I have looked into a few systems but
the cost are hugh. We are a small derm lab with one reading physician and
im the only tech. So I do not need a large system. I need to be able to
generate path reports. Accession, create reports and logs for about 3000
specimen a year. I wish I could just purchase outright but will lease if
we have to.  I havent had must luck at finding software for small labs
except Pathlogix. Love the software, not so fond of the company. Please
tell me what software is out there and If you guys like it.

Thank a million,
Nicole Tatum HT ASCP


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law.  If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy.



------------------------------

Message: 11
Date: Tue, 07 Jun 2011 15:16:00 -0400
From: mtitford@aol.com
Subject: [Histonet] Polyvinyl alcohol
To: histonet@lists.utsouthwestern.edu
Message-ID: <8CDF35E787AC92D-2684-560EF@webmail-d144.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"



Candice Camille asks about polyvinyl alcohol.

Some time ago when I was in the Army Reserve I completed a correspondence course for parasitology and it discussed PVA. I still have the manual. It states: "PVA is a mixture of fixative and water soluble resin that is specifically used to fix and preserve trophozoites of intestinal amoebic organisms. " Further on it states, " PVA is primarily used for preserving fresh specimens to be shipped to central laboratories". A formula is given. I can Fax it to you if you give me a Fax number.

Regards
Michael Titford
USA - Pathology 
Mobile  AL USA




------------------------------

Message: 12
Date: Tue, 7 Jun 2011 12:27:07 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] question on H pylori
To: "histonet@lists.utsouthwestern.edu"
	,
	"'histonet-request@lists.utsouthwestern.edu'"
	, 	LisaSetlak
	
Message-ID: <365856.95700.qm@web65703.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Modified Steiner.
Ren? J.

--- On Tue, 6/7/11, Setlak, Lisa  wrote:


From: Setlak, Lisa 
Subject: [Histonet] question on H pylori
To: "histonet@lists.utsouthwestern.edu" , "'histonet-request@lists.utsouthwestern.edu'" 
Date: Tuesday, June 7, 2011, 2:12 PM


I was just curious what everyone is using for standard of care regarding H =
? ? Pylori......is everyone doing IHC or are you doing a Giemsa?
? ? Thanks,
? ? Lisa
? ? Lisa M. Van Valkenberg, B.S., HT- ASCP
? ? Histology Manager
? ? 2300 Children's Plaza
? ? Chicago, IL 60614
? ? 773-868-8949



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 13
Date: Tue, 07 Jun 2011 15:33:49 -0400
From: "Angela Bitting" 
Subject: [Histonet] Ventana probes
To: ,
	
Message-ID: <4DEE44DE.2B7F.00C9.1@geisinger.edu>
Content-Type: text/plain; charset=US-ASCII

I have a Pathologist asking me to try running Kappa and Lambda by CISH on bone marrow smears. Does anyone have any experience with trying that?
 
Happy strange Tuesday,
Angie


IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.

------------------------------

Message: 14
Date: Tue, 7 Jun 2011 15:09:56 -0500
From: 
Subject: [Histonet] RE: question on H pylori
To: ,
	,
	
Message-ID:
	<9E2D36CE2D7CBA4A94D9B22E8328A3BA13A77E4855@NADCWPMSGCMS03.hca.corpad.net>
	
Content-Type: text/plain; charset="us-ascii"

IHC on all gastric biopsies here.

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa
Sent: Tuesday, June 07, 2011 2:12 PM
To: histonet@lists.utsouthwestern.edu; 'histonet-request@lists.utsouthwestern.edu'
Subject: [Histonet] question on H pylori

I was just curious what everyone is using for standard of care regarding H =
    Pylori......is everyone doing IHC or are you doing a Giemsa?
    Thanks,
    Lisa
    Lisa M. Van Valkenberg, B.S., HT- ASCP
    Histology Manager
    2300 Children's Plaza
    Chicago, IL 60614
    773-868-8949



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 15
Date: Tue, 7 Jun 2011 13:52:52 -0700
From: "Matt Brooks" 
Subject: RE: [Histonet] RE: question on H pylori
To: , ,
	,
	
Message-ID:
	<706224670091FE47997AEF88EFADE7CA01FA5521@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM>
	
Content-Type: text/plain;	charset="us-ascii"

We perform an IHC

Matt Brooks, BS, HT (ASCP)
Histology Supervisor
InCyte Pathology
mbrooks@incytepathology.com
509-892-2744 (W)
720-253-7204 (C)

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Wanda.Smith@HCAhealthcare.com
Sent: Tuesday, June 07, 2011 1:10 PM
To: LSetlak@childrensmemorial.org; histonet@lists.utsouthwestern.edu;
histonet-request@lists.utsouthwestern.edu
Subject: [Histonet] RE: question on H pylori

IHC on all gastric biopsies here.

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or
CONFIDENTIAL information and may be read or used only by the intended
recipient. If you are not the intended recipient of the email or any of
its attachments, please be advised that you have received this email in
error and that any use, dissemination, distribution, forwarding,
printing, or copying of this email or any attached files is strictly
prohibited. If you have received this email in error, please immediately
purge it and all attachments and notify the sender by reply email or
contact the sender at the number listed.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Setlak,
Lisa
Sent: Tuesday, June 07, 2011 2:12 PM
To: histonet@lists.utsouthwestern.edu;
'histonet-request@lists.utsouthwestern.edu'
Subject: [Histonet] question on H pylori

I was just curious what everyone is using for standard of care regarding
H =
    Pylori......is everyone doing IHC or are you doing a Giemsa?
    Thanks,
    Lisa
    Lisa M. Van Valkenberg, B.S., HT- ASCP
    Histology Manager
    2300 Children's Plaza
    Chicago, IL 60614
    773-868-8949



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 16
Date: Tue, 7 Jun 2011 14:15:10 -0700
From: Patrick Laurie 
Subject: Re: [Histonet] question on H pylori
To: "Setlak, Lisa" 
Cc: "histonet@lists.utsouthwestern.edu"
	,
	"histonet-request@lists.utsouthwestern.edu"
	
Message-ID: 
Content-Type: text/plain; charset=ISO-8859-1

IHC here.

On Tue, Jun 7, 2011 at 11:12 AM, Setlak, Lisa
 wrote:
> I was just curious what everyone is using for standard of care regarding H =
> ? ?Pylori......is everyone doing IHC or are you doing a Giemsa?
> ? ?Thanks,
> ? ?Lisa
> ? ?Lisa M. Van Valkenberg, B.S., HT- ASCP
> ? ?Histology Manager
> ? ?2300 Children's Plaza
> ? ?Chicago, IL 60614
> ? ?773-868-8949
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie@cellnetix.com



------------------------------

Message: 17
Date: Tue, 7 Jun 2011 15:15:57 -0700 (PDT)
From: SHANE NELSON 
Subject: [Histonet] Nicole Tatum -AP Software
To: Histonet@lists.utsouthwestern.edu
Message-ID: <479132.53598.qm@web84307.mail.re1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Nicole APEASY seems to be Great?Lab Software, very user friendly and very cost 
effective. Espicially when it comes down to?annual maintenance.
?
THANK YOU,
?
PATTI RUBEN-NELSON? H.T.(ASCP) 
PNP LABORATORY CONSULTANTS
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761 
nelsonrnch@verizon.net

------------------------------

Message: 18
Date: Tue, 7 Jun 2011 17:31:26 -0500
From: "Hale, Meredith" 
Subject: [Histonet] PA HT Position 
To: 
Message-ID:
	<6F33D8418806044682A391273399860F086BEC9F@s-irv-ex301.PathologyPartners.intranet>
	
Content-Type: text/plain;	charset="us-ascii"

Great opportunity for a Histotechnician in a brand new laboratory! We
are an 8 physician Dermatology practice located in Bucks County, PA
looking for a certified HT or HTL.  Candidate must meet CLIA grossing
requirements.  The candidate will be responsible for routine histology
duties.  This is a part-time position that offers a competitive salary
and flexible hours. Interested applicants should contact Meredith Hale,
phone (214)596-2219 or e-mail mhale@carisls.com

 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 214-596-7095

mhale@carisls.com   

 



------------------------------

Message: 19
Date: Tue, 7 Jun 2011 16:38:52 -0700
From: "Matt Brooks" 
Subject: [Histonet] Flow Cytometry
To: 
Cc: Janice Payne 
Message-ID:
	<706224670091FE47997AEF88EFADE7CA01FA5524@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM>
	
Content-Type: text/plain;	charset="us-ascii"

Hello All,

 

We are looking into starting performing our own Flow Cytometry.  Is
there anyone out there that is doing this and can someone recommend a
company to contact about equipment and pricing?  Your help is greatly
appreciated.

 

Thank you, 

 

Matt Brooks, BS, HT (ASCP)

Histology Supervisor

InCyte Pathology

mbrooks@incytepathology.com

509-892-2744 (W)

720-253-7204 (C)

 



------------------------------

Message: 20
Date: Tue, 7 Jun 2011 19:50:46 -0400
From: Amos Brooks 
Subject: [Histonet] Fite control block vs Block Disposal
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset=ISO-8859-1

Probably from one of these folks throwing away their blocks. A tragedy, I
tell you!

Amos


On Tue, Jun 7, 2011 at 1:00 PM,
wrote:

> Message: 9
> Date: Mon, 6 Jun 2011 15:30:33 -0400
> From: "Artim, Kimberly" 
> Subject: [Histonet] Fite control block
> To: "histonet@lists.utsouthwestern.edu"
>        
> Message-ID:
>        <9E67FDD215B226448638018A82B952BD021E7261D5BA@EXCHANGE.slhn.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone know where I can get a Fite control block?
>
> Kimberly Artim, AST, HT (ASCP)
> Technical Coordinator, Anatomic Pathology
> St Lukes Hospital & Health Network
> 801 Ostrum Street
> Bethlehem, PA 18015
> 610-954-4832
>


------------------------------

Message: 21
Date: Tue, 7 Jun 2011 16:52:08 -0700
From: Mark Tarango 
Subject: Re: [Histonet] Flow Cytometry
To: Matt Brooks 
Cc: histonet@lists.utsouthwestern.edu,	Janice Payne
	
Message-ID: 
Content-Type: text/plain; charset=ISO-8859-1

Hi Matt,

You might get better answers on the flow listserv.
http://www.cyto.purdue.edu/hmarchiv/index.htm

Good luck.

Mark

On Tue, Jun 7, 2011 at 4:38 PM, Matt Brooks wrote:

> Hello All,
>
>
>
> We are looking into starting performing our own Flow Cytometry.  Is
> there anyone out there that is doing this and can someone recommend a
> company to contact about equipment and pricing?  Your help is greatly
> appreciated.
>
>
>
> Thank you,
>
>
>
> Matt Brooks, BS, HT (ASCP)
>
> Histology Supervisor
>
> InCyte Pathology
>
> mbrooks@incytepathology.com
>
> 509-892-2744 (W)
>
> 720-253-7204 (C)
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


------------------------------

Message: 22
Date: Tue, 7 Jun 2011 20:06:15 -0400
From: Amos Brooks 
Subject: [Histonet] Re: Histonet Digest, Vol 91, Issue 9
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset=ISO-8859-1

Hi,
    You could try Newcomer Supply.
http://www.newcomersupply.com/products-positive-qc-histopathology.php They
have an AFB control that they say has "Mycobacterium sp. Tuberculosis
positive and negative tissue in same section". That should work.
Amos

On Tue, Jun 7, 2011 at 1:00 PM,
wrote:

> Message: 9
> Date: Mon, 6 Jun 2011 15:30:33 -0400
> From: "Artim, Kimberly" 
> Subject: [Histonet] Fite control block
> To: "histonet@lists.utsouthwestern.edu"
>        
> Message-ID:
>        <9E67FDD215B226448638018A82B952BD021E7261D5BA@EXCHANGE.slhn.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone know where I can get a Fite control block?
>
> Kimberly Artim, AST, HT (ASCP)
> Technical Coordinator, Anatomic Pathology
> St Lukes Hospital & Health Network
> 801 Ostrum Street
> Bethlehem, PA 18015
> 610-954-4832
>


------------------------------

Message: 23
Date: Wed, 8 Jun 2011 01:23:39 +0000
From: "Jean Brinker" 
Subject: Re: [Histonet] AP software
To: "Nicole Tatum" ,
	histonet-bounces@lists.utsouthwestern.edu,
	histonet@lists.utsouthwestern.edu
Message-ID:
	<1041701992-1307496213-cardhu_decombobulator_blackberry.rim.net-128648223-@b14.c13.bise6.blackberry>
	
Content-Type: text/plain

Nicole, we've been pretty happy with APEasy as well. 
Sent from my BlackBerry

-----Original Message-----
From: "Nicole Tatum" 
Sender: histonet-bounces@lists.utsouthwestern.edu
Date: Tue, 7 Jun 2011 14:35:47 
To: 
Subject: [Histonet] AP software

Hello Histonetters,

I need help. So I have used Pathlogix software for about 4yrs and I love
it. It so super simple and have never had any problems. Well, except for
one. The cost of leasing has increase by 19% each year for no apprearent
reason. We are now paying quarterly triple what we leased it for 4yrs ago.
The owner says cost are riseing due to the economy and prices are subject
to change without notice. Really! 19%. Cause im pretty sure thats bad
business and thats how you lose customer instead of keeping them. At any
rate, I need to find a new company. I have looked into a few systems but
the cost are hugh. We are a small derm lab with one reading physician and
im the only tech. So I do not need a large system. I need to be able to
generate path reports. Accession, create reports and logs for about 3000
specimen a year. I wish I could just purchase outright but will lease if
we have to.  I havent had must luck at finding software for small labs
except Pathlogix. Love the software, not so fond of the company. Please
tell me what software is out there and If you guys like it.

Thank a million,
Nicole Tatum HT ASCP


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 24
Date: Tue, 7 Jun 2011 22:07:18 -0400
From: Robert Richmond 
Subject: [Histonet] Re: Fite control block
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset=ISO-8859-1

Kimberly Artim, AST, HT (ASCP), Technical Coordinator, Anatomic
Pathology, St Lukes Hospital & Health Network, Bethlehem, Pennsylvania
asks about a Fite control block.

Do you mean a block of tissue with Mycobacterium leprae?

It's preferable indeed to have lepra bacilli rather than tubercle
bacilli in the control, since the two organisms stain somewhat
differently. Obviously human tissue is extremely rare. Does anyone use
leprous armadillo (Dasypus novemcinctus) tissue as a control? Does
anyone know how to get it?

Bob Richmond
Samurai Pathologist
Knoxville TN



------------------------------

Message: 25
Date: Wed, 8 Jun 2011 12:33:57 +0000
From: "Dawson, Glen" 
Subject: [Histonet] RE: question on H pylori
To: "'Setlak, Lisa'" ,
	"histonet@lists.utsouthwestern.edu"
	
Message-ID:
	
Content-Type: text/plain; charset="us-ascii"

Lisa,

We use both IHC and Warthin Starry here, depending on the Pathologist's preference.

Glen Dawson  BS, HT(ASCP) & QIHC
IHC Manager
Milwaukee, WI

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa
Sent: Tuesday, June 07, 2011 1:12 PM
To: histonet@lists.utsouthwestern.edu; 'histonet-request@lists.utsouthwestern.edu'
Subject: [Histonet] question on H pylori

I was just curious what everyone is using for standard of care regarding H =
    Pylori......is everyone doing IHC or are you doing a Giemsa?
    Thanks,
    Lisa
    Lisa M. Van Valkenberg, B.S., HT- ASCP
    Histology Manager
    2300 Children's Plaza
    Chicago, IL 60614
    773-868-8949



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 91, Issue 10
****************************************

IMPORTANT NOTICE: The contents of this message, together with any attachments, are intended for the use of the person(s) to which they are addressed and may contain confidential and/or privileged/sensitive information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution or copying of this message, or any attachment is prohibited. 
Thank you for your cooperation.


From mcauliff <@t> umdnj.edu  Wed Jun  8 15:43:46 2011
From: mcauliff <@t> umdnj.edu (Geoff McAuliffe)
Date: Wed Jun  8 15:40:15 2011
Subject: [Histonet] Pre-made paraformaldehyde question
In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB57D6@PHSXMB30.partners.org>
References: 
	<073AE2BEA1C2BA4A8837AB6C4B943D9708DB57D6@PHSXMB30.partners.org>
Message-ID: <4DEFDF02.5010306@umdnj.edu>

Suh et al.

I remember that the dogma on paraformaldehyde was that it must be made 
fresh. However, I also remember that someone has actually compared fresh 
to "old" (a week or two) and found no difference. This was published in 
a peer-reviewed journal, I just don't remember which one.

Geoff

On 6/8/2011 2:14 PM, Sherwood, Margaret wrote:
> I don't believe that anyone responded to your inquiry.  I used to make up a
> Karnovsky's fixative (for TEM) (paraformaldehyde/glutaraldehyde) using the
> powder form of paraformaldehyde.  It is highly toxic.  No one that I know of
> does this anymore (or shouldn't).  Now I buy the 16% solution of
> paraformaldehyde (comes in sealed 10ml ampules).  I also buy my glutaraldehyde
> solution that way.  Then I make whatever dilution I need.  Much safer and
> simpler this way.
>
> Peggy
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suhyoung Jeong
> Sent: Monday, June 06, 2011 8:39 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Pre-made paraformaldehyde question
>
> Dear Histo people,
>
> In my old lab, I have been taught that 4% PFA has to be always made fresh
> (maybe a day before max). Here in my new lab, I find that people use 32%
> pre-made PFA from EMS (without expiry date) and only dilute it on the day of
> use.
>
> Does anyone have experience (or thoughts) with this issue?
>
> Thank you in advance
>
> All the best, Suh
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> The information in this e-mail is intended only for the person to whom it is
> addressed. If you believe this e-mail was sent to you in error and the e-mail
> contains patient information, please contact the Partners Compliance HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in error
> but does not contain patient information, please contact the sender and properly
> dispose of the e-mail.
>
>
> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff@umdnj.edu
**********************************************



From cforster <@t> umn.edu  Wed Jun  8 17:11:01 2011
From: cforster <@t> umn.edu (Colleen Forster)
Date: Wed Jun  8 17:10:59 2011
Subject: [Histonet] In need of a Methods in Cell Biology chapter
Message-ID: <4DEFF375.9060600@umn.edu>

  Hello Histonetters,

I am wondering if anyone out there has the hard copy volumes of "Methods 
in Cell Biology"  Volume 13, pages 171-193 (which is Chapter 9). This 
chapter has methodology in micro autoradiography that I really need for 
a protocol design.

The method I need is "Techniques for the autoradiography of diffusible 
compounds by W.E. Stumpf. This volume is from 1976 so it goes way back!!

HELP!! anyone. Our medical library does not have this book and to get it 
on Amazon was $172, too steep for my pocket.....

Thanks....

Colleen Forster HT(ASCP)QIHC
U of MN
From clb1158 <@t> yahoo.com  Wed Jun  8 19:36:54 2011
From: clb1158 <@t> yahoo.com (C B)
Date: Wed Jun  8 19:36:59 2011
Subject: [Histonet] BRDU
Message-ID: <763963.19787.qm@web114002.mail.gq1.yahoo.com>

Is anyone using ph6 HEIR instead of HCl/enzyme digestion for BRDU staining?? 
I've read 2 manufacturer datasheets that recommend pH 6 HEIR.? I thought the 
routine pretreatment was HCl/enzyme digestion.??We have FFPE rat intestine to 
stain. Any feeback is appreciated.
Cindy Baranowski, HT
SJTRI
Atlanta, GA
From sonya.martin <@t> soton.ac.uk  Thu Jun  9 06:23:10 2011
From: sonya.martin <@t> soton.ac.uk (James S.)
Date: Thu Jun  9 06:23:33 2011
Subject: [Histonet] Frozen sectioning human spleen
Message-ID: <5F338719C0D0DE44BDFDD2B83D3FF7A1CBAB2F1646@UOS-CL-EX7-L3.soton.ac.uk>

I've been trying to get some frozen sections of human spleen. Usually all the work I do is with mouse tissue and I take the tissues myself and immediately freeze in OCT in isopentane/dry ice. The problem with the human samples is that I have no control over how they are frozen! I have one sample from our path dept which was just put directly in the -80oC freezer the other sample I have is from LN2 storage (I presume it was snap frozen in LN2 after removal).

Anyway the sections from both samples are awful! They seem to cut ok but if you do any staining on them they fall apart and any tissue left just doesn't look right - the nuclei look misshapen, fuzzy, stretched and extracted. In sections from the tissue stored in LN2 the nuclei were either missing or didn't take up the dapi at all (except at the very edge of the section).

Has anyone had similar problems?

I cut 10um sections, air dry overnight, fix 100% acetone 10min.

Thanks
Sonya


------------------------------------------------------------


From doakes <@t> olympicmedical.org  Thu Jun  9 07:30:57 2011
From: doakes <@t> olympicmedical.org (Dawn Oakes)
Date: Thu Jun  9 07:45:02 2011
Subject: [Histonet] Helicobacter tissue
Message-ID: 

   Does anyone in Histo- land have extra blocks they would like to trade
for either  Gram  -/+ or  Fungus  control blocks?  Thanks in advance!

 

Dawn Oakes HT ( ASCP )

Olympic Medical Center

939 Caroline Street

Port Angeles, WA 98362

360-417-7467


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From mcauliff <@t> umdnj.edu  Thu Jun  9 08:31:43 2011
From: mcauliff <@t> umdnj.edu (Geoff McAuliffe)
Date: Thu Jun  9 08:28:16 2011
Subject: [Histonet] In need of a Methods in Cell Biology chapter
In-Reply-To: <4DEFF375.9060600@umn.edu>
References: <4DEFF375.9060600@umn.edu>
Message-ID: <4DF0CB3F.1020807@umdnj.edu>

Try interlibrary loan, your library will have access.

Geoff


On 6/8/2011 6:11 PM, Colleen Forster wrote:
>  Hello Histonetters,
>
> I am wondering if anyone out there has the hard copy volumes of 
> "Methods in Cell Biology"  Volume 13, pages 171-193 (which is Chapter 
> 9). This chapter has methodology in micro autoradiography that I 
> really need for a protocol design.
>
> The method I need is "Techniques for the autoradiography of diffusible 
> compounds by W.E. Stumpf. This volume is from 1976 so it goes way back!!
>
> HELP!! anyone. Our medical library does not have this book and to get 
> it on Amazon was $172, too steep for my pocket.....
>
> Thanks....
>
> Colleen Forster HT(ASCP)QIHC
> U of MN
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff@umdnj.edu
**********************************************

From AGleiberman <@t> cbiolabs.com  Thu Jun  9 08:58:11 2011
From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman)
Date: Thu Jun  9 08:58:18 2011
Subject: [Histonet] BRDU
In-Reply-To: <763963.19787.qm@web114002.mail.gq1.yahoo.com>
References: <763963.19787.qm@web114002.mail.gq1.yahoo.com>
Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C17FC25@cbiolabs05.CBiolabs.local>

I used pH 6 HEIR instead of HCl/enzyme for BrdU - it works very well on mouse and monkey intestine. Did not switch to HCl after that. But now I prefer to use EdU instead of BrdU (Click-iT kit from Invitrogen). It is more expensive, but much more sensitive, fast, easy to use and well compatible with all range of antigens(for multi-color fluorescence). Unfortunately, there are no enzymatic reaction for EdU - only fluorescence. 

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman@cbiolabs.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C B
Sent: Wednesday, June 08, 2011 8:37 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] BRDU

Is anyone using ph6 HEIR instead of HCl/enzyme digestion for BRDU staining?  
I've read 2 manufacturer datasheets that recommend pH 6 HEIR.  I thought the 
routine pretreatment was HCl/enzyme digestion.  We have FFPE rat intestine to 
stain. Any feeback is appreciated.
Cindy Baranowski, HT
SJTRI
Atlanta, GA
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

 


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From Lisa.White3 <@t> va.gov  Thu Jun  9 09:08:42 2011
From: Lisa.White3 <@t> va.gov (White, Lisa M.)
Date: Thu Jun  9 09:08:47 2011
Subject: [Histonet] BioGenex vs Ventana
Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760830AA89@VHAV09MSGA3.v09.med.va.gov>

We are looking for anyone who has opinion/experience with BioGenex
Xmatrx & i6000 and/or Ventana Ultra & NexES.  Any input is greatly
appreciated.

 

Lisa White, HT(ASCP)

Supervisory HT

James H. Quillen VAMC

PO Box 4000

Corner of Veterans Way and Lamont

PLMS 113

Mountain Home, TN 37684

423-979-3567

423-979-3401 fax

 

From lblazek <@t> digestivespecialists.com  Thu Jun  9 09:12:09 2011
From: lblazek <@t> digestivespecialists.com (Blazek, Linda)
Date: Thu Jun  9 09:12:13 2011
Subject: [Histonet] Leica CV 5030
Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBF65E9D3@IBMB7Exchange.digestivespecialists.com>

Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using?
Thanks,
Linda


Our Vision: To be the #1 choice for all your GI services
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 396-2623
Email: lblazek@digestivespecialists.com

From Ramona_Nelson <@t> bd.com  Thu Jun  9 09:20:08 2011
From: Ramona_Nelson <@t> bd.com (Ramona_Nelson@bd.com)
Date: Thu Jun  9 09:20:19 2011
Subject: [Histonet] AUTO: Ramona Nelson is out of the office. (returning
	06/13/2011)
Message-ID: 


   


   I am out of the office until 06/13/20   

   
I    

   

   
Note:  This  is  an  automated  response  to  your message &quo   t;Histonet  Digest,  Vol  91,  Issue  12"   AM.
      
This  is  the  only  notification  you will receive whi   person is away.
   

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From wbenton <@t> cua.md  Thu Jun  9 09:23:04 2011
From: wbenton <@t> cua.md (Walter Benton)
Date: Thu Jun  9 09:23:41 2011
Subject: [Histonet] RE: Leica CV 5030
In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EBF65E9D3@IBMB7Exchange.digestivespecialists.com>
References: <5A2BD13465E061429D6455C8D6B40E390EBF65E9D3@IBMB7Exchange.digestivespecialists.com>
Message-ID: <0B8979A204680A42B93A52B486088CD91D6EC7CF0E@CUAEXH1.GCU-MD.local>

 Azer Toluene Based Mountant with the 21 needle

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wbenton@cua.md
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda [lblazek@digestivespecialists.com]
Sent: Thursday, June 09, 2011 10:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica CV 5030

Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using?
Thanks,
Linda


Our Vision: To be the #1 choice for all your GI services
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 396-2623
Email: lblazek@digestivespecialists.com

_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law.  If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy.

From trathborne <@t> somerset-healthcare.com  Thu Jun  9 09:17:15 2011
From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni)
Date: Thu Jun  9 09:26:30 2011
Subject: [Histonet] RE: Leica CV 5030
In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EBF65E9D3@IBMB7Exchange.digestivespecialists.com>
References: <5A2BD13465E061429D6455C8D6B40E390EBF65E9D3@IBMB7Exchange.digestivespecialists.com>
Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570701FB63@SMCMAIL01.somerset-healthcare.com>

We are using the needle that was supplied with the stainer. We use the Richard Allen mounting medium, preceded by Richard Allen Clear Rite 3 in the last station.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Thursday, June 09, 2011 10:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica CV 5030

Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using?
Thanks,
Linda


Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 396-2623
Email: lblazek@digestivespecialists.com

_______________________________________________
Histonet mailing list
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From gayle.callis <@t> bresnan.net  Thu Jun  9 10:01:52 2011
From: gayle.callis <@t> bresnan.net (gayle callis)
Date: Thu Jun  9 10:02:02 2011
Subject: [Histonet] Frozen sectioning human spleen
In-Reply-To: <5F338719C0D0DE44BDFDD2B83D3FF7A1CBAB2F1646@UOS-CL-EX7-L3.soton.ac.uk>
References: <5F338719C0D0DE44BDFDD2B83D3FF7A1CBAB2F1646@UOS-CL-EX7-L3.soton.ac.uk>
Message-ID: <001801cc26b6$2c3d73f0$84b85bd0$@callis@bresnan.net>

Dear Sonya, 

You need to find out how these people ARE snap freezing, then train them to
do it correctly. I have seen Histonet messages where people have success
freezing the way you described and had no problems or so it seemed although
I do not care for freezer freezing. If the liver is embedded in OCT in a
cryomold and the bottom of the cryomold is lowered slowly into LN2, they may
have better results. Just don't drop the mold into the LN2 or the OCT/tissue
may crack.  Freezer freezing at anytime due to slower freezing that creates
some huge ice crystal damage e.g. freeze artifact.  I will be happy to send
you an excellent discussion of freezing artifact from Dr. Charles Scouten if
you wish, found on the web. These people may be waiting too long before
freezing, or doing some funky little thing to create artifact. Whatever is
being done obviously is ruining the nuclei in center of sample but not the
edges where freezing is probably occurring much faster.  All artifact may
not be due to freezing either, although you have experience with murine
spleen.   
   
The thicker sections at 10 um could be pulling the nuclei out of the tissue
itself. Trimming too thick e.g. at a thickness exceeding 15 um or so, can
also do the same thing, especially at cold temperatures for this very
homogenous tissue.  Spleen contains a lot of blood, which doesn't section
well.  

Some suggestion for these problem these spleen samples.  If you already do
this, you can ignore or review: 

Correct snap freezing, although you can try this with the blocks from the
not exactly known freezing methods.

Set cryostat at -16C to -17C.  Warmer temperature allows for better
sectioning of homogenous spleen, also liver, brain, and spinal cord. 
Trim the block at thinner coarse setting.  Start trim with rapid coarse
advance until you get to the OCT, the trim into the tissue with fine
approx.15 um increments.  Do a final few turns of the flywheel at final 5 um
sectioning thickness.  This provides a smooth, clean block face with tissue
left intact, not with little chunks pulled out. 
Using a brand new disposable blade, section at 5 um.  I assume you use Plus
charge slides (you didn't say you used these?) 
To check that your sectioning is working well, do a quick Hematoxylin stain
on a section.  Pick up, fix with 95% ethanol, rinse, dip in hematoxylin 15
or more times, rinse, blue and coverslip.  Take a look at your morphology to
see if you have corrected the problem as nuclei should be intact. 
Continue with sectioning then proceed with your drying/fixation. 

Let us know if anything improves, and good luck on training the other labs. 

Good luck

Gayle M. Callis 
HTL/HT/MT(ASCP)  

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James S.
Sent: Thursday, June 09, 2011 5:23 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Frozen sectioning human spleen

I've been trying to get some frozen sections of human spleen. Usually all
the work I do is with mouse tissue and I take the tissues myself and
immediately freeze in OCT in isopentane/dry ice. The problem with the human
samples is that I have no control over how they are frozen! I have one
sample from our path dept which was just put directly in the -80oC freezer
the other sample I have is from LN2 storage (I presume it was snap frozen in
LN2 after removal).

Anyway the sections from both samples are awful! They seem to cut ok but if
you do any staining on them they fall apart and any tissue left just doesn't
look right - the nuclei look misshapen, fuzzy, stretched and extracted. In
sections from the tissue stored in LN2 the nuclei were either missing or
didn't take up the dapi at all (except at the very edge of the section).

Has anyone had similar problems?

I cut 10um sections, air dry overnight, fix 100% acetone 10min.

Thanks
Sonya


------------------------------------------------------------


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From jqb7 <@t> cdc.gov  Thu Jun  9 10:05:40 2011
From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID))
Date: Thu Jun  9 10:06:27 2011
Subject: [Histonet] RE: Leica CV 5030
In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EBF65E9D3@IBMB7Exchange.digestivespecialists.com>
References: <5A2BD13465E061429D6455C8D6B40E390EBF65E9D3@IBMB7Exchange.digestivespecialists.com>
Message-ID: 

We use Cytoseal XYL and a 21 gauge nozzle.  We were provided a variety of different size nozzles...which one you use depends on which mountant you use.

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Thursday, June 09, 2011 10:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica CV 5030

Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using?
Thanks,
Linda


Our Vision: To be the #1 choice for all your GI services
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 396-2623
Email: lblazek@digestivespecialists.com

_______________________________________________
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From HornHV <@t> archildrens.org  Thu Jun  9 10:15:03 2011
From: HornHV <@t> archildrens.org (Horn, Hazel V)
Date: Thu Jun  9 10:15:22 2011
Subject: [Histonet] RE: Leica CV 5030
In-Reply-To: <0B8979A204680A42B93A52B486088CD91D6EC7CF0E@CUAEXH1.GCU-MD.local>
References: <5A2BD13465E061429D6455C8D6B40E390EBF65E9D3@IBMB7Exchange.digestivespecialists.com>
	<0B8979A204680A42B93A52B486088CD91D6EC7CF0E@CUAEXH1.GCU-MD.local>
Message-ID: <25A4DE08332B19499904459F00AAACB71987E03DCB@EVS1.archildrens.org>

We use Richard Allan Scientific mounting media with a 21 gauge needle.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton
Sent: Thursday, June 09, 2011 9:23 AM
To: Blazek, Linda; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Leica CV 5030

 Azer Toluene Based Mountant with the 21 needle

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wbenton@cua.md
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda [lblazek@digestivespecialists.com]
Sent: Thursday, June 09, 2011 10:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica CV 5030

Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using?
Thanks,
Linda


Our Vision: To be the #1 choice for all your GI services
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 396-2623
Email: lblazek@digestivespecialists.com

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law.  If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy.

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Thank you.

From aevans3 <@t> lghealth.org  Thu Jun  9 10:53:50 2011
From: aevans3 <@t> lghealth.org (Evans, Andria B)
Date: Thu Jun  9 10:55:43 2011
Subject: [Histonet] HPV
Message-ID: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA84@MAIL-AG-CLUSTER.lha.org>

The pathologist here would like to get HPV up and running in house.  I'm just asking for some feed back from everyone that is running HPV (manufacture; clone; protocol).  We currently run the Ventana Benchmark XT/Ultra platform.  Any help would be greatly appreciated.

Andrea
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From Rcartun <@t> harthosp.org  Thu Jun  9 11:02:04 2011
From: Rcartun <@t> harthosp.org (Richard Cartun)
Date: Thu Jun  9 11:02:13 2011
Subject: [Histonet] HPV
In-Reply-To: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA84@MAIL-AG-CLUSTER.lha.org>
References: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA84@MAIL-AG-CLUSTER.lha.org>
Message-ID: <4DF0B63B.7400.0077.1@harthosp.org>

You need to use in situ hybridization for HPV detection.  IHC is not sensitive or specific for routine use.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Evans, Andria B"  6/9/2011 11:53 AM >>>
The pathologist here would like to get HPV up and running in house.  I'm just asking for some feed back from everyone that is running HPV (manufacture; clone; protocol).  We currently run the Ventana Benchmark XT/Ultra platform.  Any help would be greatly appreciated.

Andrea
This email was sent securely from the LGHealth Email Service

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Any unauthorized review, use, disclosure or distribution is
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From rsrichmond <@t> gmail.com  Thu Jun  9 11:10:00 2011
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Thu Jun  9 11:10:05 2011
Subject: [Histonet] Re: Manger position in Torrance CA
Message-ID: 

Re "Manger position in Torrance CA":

Is the applicant required to eat hay? Is donkey work involved?

Bob Richmond
Samurai Pathologist
Knoxville TN

From LINDA.MARGRAF <@t> childrens.com  Thu Jun  9 11:30:03 2011
From: LINDA.MARGRAF <@t> childrens.com (Linda Margraf)
Date: Thu Jun  9 11:30:09 2011
Subject: [Histonet] Histology position at Caris
Message-ID: <683621D7852C2F488898D0AC7F164A98882DDFD0@CMCPBEXMAIL02.Childrens.med>

Here is a job I am posting for Crystal.  Please contact her (info at bottom of message)and not me about it.

Thanks

Linda M,  Histonet administrator


Caris Life Sciences, Inc., a leading biosciences company focused on enabling precise and personalized healthcare through the highest quality anatomic pathology, molecular profiling, and blood-based diagnostic services has an opening for a histotechnologist.

Essential Job Functions:

Histotechnologists will utilize the knowledge necessary to assist pathology professionals with Immunohistochemistry, specimen processing, embedding, slide preparation, microtomy, and staining, special and other technical procedures performed in the laboratory.

  *   Perform routine and non-routine activities involved in the preparation of slides for microscopic evaluation by pathologist(s), according to policies and procedures.
  *   Duties and tasks are standardized.
  *   Perform routine maintenance and cleaning of equipment and troubleshoot minor equipment failures.  Document remedial actions such as repairs or reported tests.
  *   Adhere to laboratory's quality control policies and document all quality control activities.
  *   Ensure all corporate safety, quality control and quality assurance standards are met.
  *   Ensure compliance with all local, federal, CLIA and CAP regulations.
Job Requirements:

*         Bachelor's Degree in Science or equivalent experience

*         HT/HTL (ASCP) certification preferred but not required.

*         1-2 years of experience in position or specialization
Locations: Irving, TX; Phoenix, AZ; Boston, MA
Crystal Flowers-Graves
Manager, Corporate Recruiting

Caris Life Sciences
6655 N. MacArthur Blvd.
Irving, TX 75039
(214) 596-7003 Direct
cflowers@carisdx.com
www.carisdx.com



Please consider the environment before printing this e-mail

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information that is confidential and privileged. This information is intended only for the use of the
individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further
disclosures are prohibited without proper authorization. If you are not the intended recipient, any
disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
violation of federal or state law and regulations. If you have received this information in error,
please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at
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Please consider the environment before printing this e-mail

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disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
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From kiran_g <@t> sbcglobal.net Thu Jun 9 11:31:49 2011 From: kiran_g <@t> sbcglobal.net (kiran_g@sbcglobal.net) Date: Thu Jun 9 11:31:53 2011 Subject: [Histonet] TMA instrument Message-ID: <2004137248-1307637110-cardhu_decombobulator_blackberry.rim.net-1623857192-@b4.c14.bise6.blackberry> Need help to find best TMA instrument available in the histo world. Any feedback or recommendations are welcome. Thank you, Kiran Sent from my Verizon Wireless BlackBerry From MSHERWOOD <@t> PARTNERS.ORG Thu Jun 9 12:42:48 2011 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Jun 9 12:42:54 2011 Subject: [Histonet] Leica CV 5030 In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EBF65E9D3@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E390EBF65E9D3@IBMB7Exchange.digestivespecialists.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB57E4@PHSXMB30.partners.org> Linda, I addressed this issue before. The smallest gauge needle that Leica supplies for the CV5030 is a 21 gauge needle which we found to be too big (too much mounting media came out and made a mess of the slides). We found a company on the internet that sells re-usable metal needles in all sizes. The link is: www.dispensinglink.com We got a 23 gauge needle (1/2 inch shaft) pk/12 for @$25.00. We tried different mounting media, currently use permount (in a ratio 4 parts Permount : 1 part CitriSolv). Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 09, 2011 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica CV 5030 Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using? Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From lblazek <@t> digestivespecialists.com Thu Jun 9 12:55:22 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jun 9 12:55:33 2011 Subject: [Histonet] Leica CV 5030 In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB57E4@PHSXMB30.partners.org> References: <5A2BD13465E061429D6455C8D6B40E390EBF65E9D3@IBMB7Exchange.digestivespecialists.com> <073AE2BEA1C2BA4A8837AB6C4B943D9708DB57E4@PHSXMB30.partners.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBF65E9D8@IBMB7Exchange.digestivespecialists.com> Thanks again for the information Peggy. I did have your email on your needles and mounting media. I was interested in what everyone else was using also since we are now ready to order a different type of mounting media than what we are currently using. -----Original Message----- From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] Sent: Thursday, June 09, 2011 1:43 PM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica CV 5030 Linda, I addressed this issue before. The smallest gauge needle that Leica supplies for the CV5030 is a 21 gauge needle which we found to be too big (too much mounting media came out and made a mess of the slides). We found a company on the internet that sells re-usable metal needles in all sizes. The link is: www.dispensinglink.com We got a 23 gauge needle (1/2 inch shaft) pk/12 for @$25.00. We tried different mounting media, currently use permount (in a ratio 4 parts Permount : 1 part CitriSolv). Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 09, 2011 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica CV 5030 Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using? Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Jun 9 13:20:02 2011 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Jun 9 13:20:33 2011 Subject: [Histonet] HPV In-Reply-To: <4DF0B63B.7400.0077.1@harthosp.org> References: <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA84@MAIL-AG-CLUSTER.lha.org>, <4DF0B63B.7400.0077.1@harthosp.org> Message-ID: That is what we are using here. We are no longer using the HPV antibody. It never really worked well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun@harthosp.org] Sent: Thursday, June 09, 2011 12:02 PM To: Andria B Evans; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HPV You need to use in situ hybridization for HPV detection. IHC is not sensitive or specific for routine use. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Evans, Andria B" 6/9/2011 11:53 AM >>> The pathologist here would like to get HPV up and running in house. I'm just asking for some feed back from everyone that is running HPV (manufacture; clone; protocol). We currently run the Ventana Benchmark XT/Ultra platform. Any help would be greatly appreciated. Andrea This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diane.Craft <@t> amcny.org Thu Jun 9 17:43:36 2011 From: Diane.Craft <@t> amcny.org (Diane.Craft@amcny.org) Date: Thu Jun 9 17:37:12 2011 Subject: [Histonet] Grimelius protocol for small animals in NY Message-ID: Hello All, I am fairly new to the world of special stains. My specialty is immunohistochemistry. But from what I understand, our lab and many other animal labs in New York have been unsuccessful in getting the Grimelius stain to work. Does anybody from New York have a working protocol? Thanks! Diane Craft Pathology Department Animal Medical Center 510 East 62nd St New York NY 10065-8314 212-329-8675 (phone) 212-759-5878 (fax) From alineumann <@t> aol.com Fri Jun 10 02:40:12 2011 From: alineumann <@t> aol.com (alineumann@aol.com) Date: Fri Jun 10 02:40:36 2011 Subject: [Histonet] AFB stains, shiny water artifact, in resource limited countries Message-ID: <8CDF558C41E867D-17AC-1C7F8@Webmail-d116.sysops.aol.com> Hi, I am volunteering in a 300 bed Kenya hospital for a month (Kijabe), and we have a problem with numerous shiny red artifacts, some of which are the shape and size of TB (which are very distracting), apparently caused by processing with 70% alcohol. Does anyone know a way to eliminate these while still using 70% isopropryl, which is apparently the only economically feasible processing reagent available here? Would it help to soak the blocks in 95% before cutting (we have small amounts of 95% available). Thanks very much in advance! Alice Alice Neumann MD Western Wyoming Pathology PC Pinnacle Pathology PC 9423 West Kentucky Place Lakewood, CO 80226 Phone: 303-989-3098 24 Hour Cell Phone: 307-413-4092 alineumann@aol.com From Sara.Lees <@t> covance.com Fri Jun 10 02:51:30 2011 From: Sara.Lees <@t> covance.com (Lees, Sara) Date: Fri Jun 10 02:51:48 2011 Subject: [Histonet] Grimelius stain on gut + Chromogranin Message-ID: <248907F494F5B740A86B9C6FF4C3D59F5F130B@crwxch04.ent.covance.com> Hi All, Just wanted to know if anyone had any suggestions on how I could improve the sensitivity of this stain as my last effort was very weak, also does anyone have any good methods for a chromogranin on mouse tissue. Kind Regards Sara ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From PAMarcum <@t> uams.edu Fri Jun 10 08:09:51 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Jun 10 08:09:55 2011 Subject: [Histonet] PRN Needed in Little Rock Message-ID: Good Morning, University of Arkansas for Medical Sciences is looking for a PRN Histologist (must be HT registered) for days with an occasional possible night shift. Please contact me with your information to be considered. E-mail is preferred as I am rarely in my office. This will be an ongoing position on an as needed basis for a minimum of one year. Best Regards, Pamela A Marcum AP Histology/Gross Room Supervisor 4301 W Markham Street Little Rock AR Office: 501-686-5341 Fax: 501-686-7151 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. From b-frederick <@t> northwestern.edu Fri Jun 10 08:15:55 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Jun 10 08:15:59 2011 Subject: [Histonet] Leica CV 5030 In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EBF65E9D3@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E390EBF65E9D3@IBMB7Exchange.digestivespecialists.com> Message-ID: <005b01cc2770$88085690$981903b0$@northwestern.edu> Micromount (Surgipath- now a part of Leica). 21 gauge needle. No problem. Make sure volume set is correct for the media. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 09, 2011 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica CV 5030 Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using? Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sandra.Harrison3 <@t> va.gov Fri Jun 10 09:10:07 2011 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Fri Jun 10 09:10:14 2011 Subject: [Histonet] question on H pylori In-Reply-To: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org> References: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org> Message-ID: We do either IHC or a Modified Steiner, depending on the Pathologist's preference. We switched to Newcomer Supply's Steiner-Chapman Modified Silver Stain Kit about a year ago, because it eliminated the use of Uranyl Nitrate. We have been delighted with the consistency of the stain. Sandy Harrison, HTL (ASCP) Anatomical and Surgical Pathology Supervisor, VAMC Minneapolis 612-467-2449 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa Sent: Tuesday, June 07, 2011 1:12 PM To: histonet@lists.utsouthwestern.edu; 'histonet-request@lists.utsouthwestern.edu' Subject: [Histonet] question on H pylori I was just curious what everyone is using for standard of care regarding H = Pylori......is everyone doing IHC or are you doing a Giemsa? Thanks, Lisa Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Jun 10 09:18:48 2011 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Jun 10 09:18:52 2011 Subject: [Histonet] question on H pylori In-Reply-To: References: <7111DB39D045004C9CF29E79C71B28BC10212DFFC1@CMHEXCC01MBX.childrensmemorial.org> Message-ID: <007f01cc2779$506b30f0$f14192d0$@northwestern.edu> We did diif -quik or Alcian Yellow. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Friday, June 10, 2011 9:10 AM To: Setlak, Lisa; histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu Subject: RE: [Histonet] question on H pylori We do either IHC or a Modified Steiner, depending on the Pathologist's preference. We switched to Newcomer Supply's Steiner-Chapman Modified Silver Stain Kit about a year ago, because it eliminated the use of Uranyl Nitrate. We have been delighted with the consistency of the stain. Sandy Harrison, HTL (ASCP) Anatomical and Surgical Pathology Supervisor, VAMC Minneapolis 612-467-2449 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa Sent: Tuesday, June 07, 2011 1:12 PM To: histonet@lists.utsouthwestern.edu; 'histonet-request@lists.utsouthwestern.edu' Subject: [Histonet] question on H pylori I was just curious what everyone is using for standard of care regarding H = Pylori......is everyone doing IHC or are you doing a Giemsa? Thanks, Lisa Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jun 10 10:17:06 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 10 10:17:13 2011 Subject: [Histonet] Grimelius protocol for small animals in NY In-Reply-To: References: Message-ID: <904131.36960.qm@web65705.mail.ac4.yahoo.com> Diane: Under separate cover I am sending you?the Grimelius protocol I used (even?when I never worked in New York and even when that is of no relevance!). Ren? J. From: "Diane.Craft@amcny.org" To: histonet@lists.utsouthwestern.edu Sent: Thursday, June 9, 2011 6:43 PM Subject: [Histonet] Grimelius protocol for small animals in NY Hello All, I am fairly new to the world of special stains. My specialty is immunohistochemistry. But from what I understand, our lab and many other animal labs in New York have been unsuccessful in getting the Grimelius stain to work. Does anybody from New York have a working protocol? Thanks! Diane Craft Pathology Department Animal Medical Center 510 East 62nd St New York NY 10065-8314 212-329-8675 (phone) 212-759-5878 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amario3 <@t> uic.edu Fri Jun 10 13:27:33 2011 From: amario3 <@t> uic.edu (Andrea Marion) Date: Fri Jun 10 13:27:37 2011 Subject: [Histonet] BRDU In-Reply-To: <20110610170049.EB91213041A1@barracuda.uic.edu> References: <20110610170049.EB91213041A1@barracuda.uic.edu> Message-ID: Yes, I am also using pH 6 HIER with 10mM sodium citrate, 0.05% Tween-20. I do 20 minutes at 95 degree C to retrieve BrdU on mouse embryo tissues. I use Abcam ab6326. Their product sheet states that you may used heat for antigen retrieval. The BrdU epitope needs to be revealed by denaturing the double-stranded DNA - and you can do that with an enzyme, acid, or heat - so if you are already doing HIER, the acid/enzyme treatment can be unnecessary (and may even damage the tissue/other antigens you are interested in). Andrea Marion Graduate Student University of Illinois at Chicago I used pH 6 HEIR instead of HCl/enzyme for BrdU - it works very well on mouse and monkey intestine. Did not switch to HCl after that. But now I prefer to use EdU instead of BrdU (Click-iT kit from Invitrogen). It is more expensive, but much more sensitive, fast, easy to use and well compatible with all range of antigens(for multi-color fluorescence). Unfortunately, there are no enzymatic reaction for EdU - only fluorescence. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman <@t> cbiolabs.com -----Original Message----- From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of C B Sent: Wednesday, June 08, 2011 8:37 PM To: Histonet <@t> lists.utsouthwestern.edu Subject: [Histonet] BRDU Is anyone using ph6 HEIR instead of HCl/enzyme digestion for BRDU staining? I've read 2 manufacturer datasheets that recommend pH 6 HEIR. I thought the routine pretreatment was HCl/enzyme digestion. We have FFPE rat intestine to stain. Any feeback is appreciated. Cindy Baranowski, HT SJTRI Atlanta, GA _______________________________________________ Histonet mailing list Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From trathborne <@t> somerset-healthcare.com Fri Jun 10 13:39:37 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Jun 10 13:40:29 2011 Subject: [Histonet] RE: Re:Peloris users In-Reply-To: <7111DB39D045004C9CF29E79C71B28BC10212DFFBF@CMHEXCC01MBX.childrensmemorial.org> References: <7111DB39D045004C9CF29E79C71B28BC10212DFFBF@CMHEXCC01MBX.childrensmemorial.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570701FFCA@SMCMAIL01.somerset-healthcare.com> I'm wondering which baskets were used (the ones with the spacers?) and which cassettes (the ones with the solid sides, or slotted?). Does the problem occur without using these recommended items? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa Sent: Tuesday, June 07, 2011 1:46 PM To: 'Seguin, Suzanne'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Re:Peloris users Wow, I feel like I'm listening to myself speak. We have the machine for about the same amount of time as yourself and yes we have had tissue contamination. I can't say it's super bad but we've had several cases recently where as before we rarely ever had it. Unfortunately I'm not sure what causes it and I think it got blamed on the grossing person or embedder in our cases. I'm interested to see what everyone has to say about this. Lisa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Seguin, Suzanne Sent: Tuesday, June 07, 2011 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:Peloris users Hello, We have been using this tissue processor for 1 year now. During the last 3-6 months, we have experienced an unreasonable number of cases reported with tissue contaminant in paraffin blocks. We use regular tissue cassettes as well as the biopsy and micromesh style. While using this processor I have also witnessed tissue fragment into smaller pieces while processing. Because the Peloris uses a relatively large magnetic stirrer, I'm wondering if this could be a cause for the contaminant. Has anyone else experienced this? Any thoughts, suggestions? Thanks Sue Charge Technologist - Anatomic Pathology Sudbury Regional Hospital 41 Ramsey Lake Road, Sudbury, ON P3E 5J1 (Tel (705) 523-7100 ext. 1209 Fax (705) 675-4776 sseguin@hrsrh.on.ca ************************************************************************ The information contained in this e-mail and document(s) attached are for the exclusive use of the addressee and may contain confidential, privileged and non-disclosable information. If the recipient of this e-mail is not the addressee, such recipient is strictly prohibited from reading, photocopying, distributing or otherwise using this e-mail or its content in any way. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From sadey <@t> hotmail.ca Fri Jun 10 15:22:47 2011 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Fri Jun 10 15:22:51 2011 Subject: [Histonet] Processing schedule for fatty tissues Message-ID: Hello Everyone: I am looking for a processing schedule for fatty specimens. Could anyone share theirs? Thanks in Advance. :) Sheila Bluewater Health From cbrya <@t> lexclin.com Fri Jun 10 15:35:30 2011 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Fri Jun 10 15:35:35 2011 Subject: [Histonet] Processing schedule for fatty tissues In-Reply-To: References: Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF05CB365E49@EXCHANGESB> Please respond to everyone. I would like to have the information also. Thank you, Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Friday, June 10, 2011 4:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing schedule for fatty tissues Hello Everyone: I am looking for a processing schedule for fatty specimens. Could anyone share theirs? Thanks in Advance. :) Sheila Bluewater Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From rjbuesa <@t> yahoo.com Fri Jun 10 15:36:40 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 10 15:36:43 2011 Subject: [Histonet] Processing schedule for fatty tissues In-Reply-To: References: Message-ID: <167075.91404.qm@web65702.mail.ac4.yahoo.com> The best approach is to increase the fixation time to make sure that the tissues are really fixed. Then cut the dehydration times of the lower alcohols?by 20% and add that 20% of saved time as increase in the clearing agent (supposedly xylene or alkane substitute) times. Ren? J. From: Sheila Adey To: histonet@lists.utsouthwestern.edu Sent: Friday, June 10, 2011 4:22 PM Subject: [Histonet] Processing schedule for fatty tissues Hello Everyone: I am looking for a processing schedule for fatty specimens. Could anyone share theirs? Thanks in Advance. :) Sheila Bluewater Health? ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccrowder <@t> vetmed.lsu.edu Fri Jun 10 18:06:39 2011 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Fri Jun 10 18:11:17 2011 Subject: [Histonet] Grimelius stain Message-ID: Sara- We stained many tissues for chromogranin and found that the Churukian stain worked a lot better than the Grimelius. It double impregnates the cells with the silver. Directions are in Carson's book, but if you don't have it, I can send them to you. Cheryl Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225) 772-2865 From whitmorel <@t> mindspring.com Fri Jun 10 20:15:24 2011 From: whitmorel <@t> mindspring.com (whitmorel@mindspring.com) Date: Fri Jun 10 23:30:27 2011 Subject: [Histonet] Re: histonet Message-ID: Hey histonet how's your day going? I just wanted to share this opportunity with you , I am netting about $250 dollars per day. Once you read this news article it will change your life $$$! :) http://t.co/N6PlVGZ From sadey <@t> hotmail.ca Sat Jun 11 06:21:59 2011 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Sat Jun 11 06:22:04 2011 Subject: [Histonet] RE: Distribution of work for older generation of Histotechs In-Reply-To: References: <4A53F9A1D7C2674FA4A6E650D703DDA5FB74FBAD@MSGWSDCPMB07.nyumc.org>, Message-ID: I have a 60 yr old awesome tech in the grossing room. She is wonderful and is a great teacher for the new younger techs. She works straight 9-5 everyday. I value her experience and want to keep her work environment as accomodating as possible. I also have a wonderful lady that only works in the microtomy room, again same theory with keeping her happy. The new younger ones do rotate every bench. I see how unhappy the older techs in core lab are b/c they have to work equal nights with the newbies. I believe there should be some appreciation for the ones that have been there for 30 plus yrs. Techs are waiting in line to work in our dept. I also love my job b/c the ppl I work with are so great. :) > From: PAMarcum@uams.edu > To: Milton.Gomez@nyumc.org; histonet@lists.utsouthwestern.edu > Date: Mon, 6 Jun 2011 08:51:52 -0500 > CC: > Subject: [Histonet] RE: Distribution of work for older generation of Histotechs > > We make every attempt to have all of the histologist at UAMS do the same rotations and jobs no matter the age. It is sometimes difficult due to some internal changes in the locations of the Gross Room and Histology (literally a quarter mile apart) however; we all work with what we have in these areas. Occasionally someone will object to doing a job and it is explained we all do everything as we are short handed and have no choice. > > Pam Marcum > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gomez, Milton > Sent: Sunday, June 05, 2011 12:09 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Distribution of work for older generation of Histotechs > > Hello Histonetters, > > Is the distribution of work different for older histotechs vs. younger histotechs in your labs and why? Do they get special assignments or duties because of their growing wisdom and seniority? > > Thanks in advance, > > MG > > > > ------------------------------------------------------------
> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
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> _______________________________________________
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> Confidentiality Notice: This e-mail message, including any attachments,
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From sadey <@t> hotmail.ca  Sat Jun 11 06:53:14 2011
From: sadey <@t> hotmail.ca (Sheila Adey)
Date: Sat Jun 11 06:53:18 2011
Subject: [Histonet] sections falling off slides
In-Reply-To: <863799.66085.qm@web65703.mail.ac4.yahoo.com>
References: <003601cc13f4$0f0908a0$2d1b19e0$@com>,
	<863799.66085.qm@web65703.mail.ac4.yahoo.com>
Message-ID: 


I've never worked with goat knees, but we were experiencing lots of PIN4 prostate biopsies floating off during IHC staining with HEIR.
Since we started cutting the prostate bx's at 3 micons, huge difference. They almost never float off. :)
 
> Date: Mon, 16 May 2011 13:23:02 -0700
> From: rjbuesa@yahoo.com
> To: robin_dean@compbio.com; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] sections falling off slides
> CC: 
> 
> Your test subject is one of the more difficult there are.
> Although you may get some background, use some gelatin dissolved in the water bath to try to adhere the sections to the slides. Also try to cut as thin as you can and make sure there is no water left between the sections and the slide.
> Bake at 60?C for at least 45 minutes before starting the IHC procedure. If you use HIER try to switch to pepsin.
> The least "stress" you add to the sections the better.
> Ren? J.
> 
> From: Robin Dean 
> To: histonet@lists.utsouthwestern.edu
> Sent: Monday, May 16, 2011 2:07 PM
> Subject: [Histonet] sections falling off slides
> 
> Hi All,
> 
> 
> 
> We are trying to do IHC for multiple antigens on goat knees. The sections
> are decalcified FFPE sections and we are using a Dako autostainer. We are
> currently trying air-drying for  1-2 days  and baking of tissue slides
> before use in IHC..
> 
> The one previous time we tried baking, we noticed a decrease in IHC signal.
> The sections are pretty large, we tried cutting them down, but can't
> decrease the size much.  We also  decreased the number of washes and size of
> washes on the Daok, which helped only slightly.  Does anyone have any
> suggestions?
> 
> 
> 
> Thank you in advance.
> 
> 
> 
> Robin
> 
> 
> 
> Robin R. Dean, Ph.D.
> 
> Senior Scientist & Study Director
> 
> Comparative Biosciences, Inc.
> 
> 786 Lucerne Dr.
> 
> Sunnyvale, CA
> 
> (408) 738-8060
> 
> robin_dean@compbio.com
> 
> 
> 
> _______________________________________________
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> _______________________________________________
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From a.byrnes <@t> accelpath.com  Sat Jun 11 10:42:31 2011
From: a.byrnes <@t> accelpath.com (Andrew Byrnes)
Date: Sat Jun 11 10:42:40 2011
Subject: [Histonet] EMR annual fee?
Message-ID: <52F20617-EC2C-4CCB-9B55-49E92647CE23@accelpath.com>

Does anyone know the annual support fee on EMR?  (approx?)

Thanks


Andrew Byrnes
VP Sales and Marketing
AccelPath, LLC
M: 732-312-8008
www.AccelPath.com



From bradley.miller <@t> ttuhsc.edu  Sat Jun 11 18:24:20 2011
From: bradley.miller <@t> ttuhsc.edu (Miller, Bradley)
Date: Sat Jun 11 18:24:22 2011
Subject: [Histonet] Plastic Frozen Section Chucks
Message-ID: 

Does anyone know where to find plastic (single-use) frozen section chucks?  Some of our cancer researchers would like to retain the frozen sections on the chucks so that they don't have to lose any tissue remounting and leveling in on recuts.  I thought I had heard about hard plastic chucks that could be used for this purpose, but we can't find them anywhere.


Yours Truly,

Brad


Bradley Miller M.D., Ph.D.
Assistant Professor
Departments of Pathology and Neurology
Texas Tech University Health Sciences Center
Lubbock, Texas 79424

From abright <@t> brightinstruments.com  Sun Jun 12 03:24:23 2011
From: abright <@t> brightinstruments.com (abright@brightinstruments.com)
Date: Sun Jun 12 03:24:33 2011
Subject: [Histonet] Plastic Frozen Section Chucks
In-Reply-To: 
References: 
Message-ID: <1968498838-1307867064-cardhu_decombobulator_blackberry.rim.net-2050663496-@b26.c2.bise7.blackberry>

Dear Professor Bradley,
We manufacture plastic chucks  and quick release holders to suit, mainly for skin studies. The chucks fit plastic bottles for storage in LN2 and have one way insertion tags for accurate reuse location in the quick release holder.
Best regards

Alan Bright
Bright Instrument co.
Cambs, England.

www.brightinstruments.com
Sent from my BlackBerry? wireless device

-----Original Message-----
From: "Miller, Bradley" 
Sender: histonet-bounces@lists.utsouthwestern.edu
Date: Sat, 11 Jun 2011 18:24:20 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Plastic Frozen Section Chucks

Does anyone know where to find plastic (single-use) frozen section chucks?  Some of our cancer researchers would like to retain the frozen sections on the chucks so that they don't have to lose any tissue remounting and leveling in on recuts.  I thought I had heard about hard plastic chucks that could be used for this purpose, but we can't find them anywhere.


Yours Truly,

Brad


Bradley Miller M.D., Ph.D.
Assistant Professor
Departments of Pathology and Neurology
Texas Tech University Health Sciences Center
Lubbock, Texas 79424

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From dr_sadushe <@t> yahoo.com  Sun Jun 12 07:21:46 2011
From: dr_sadushe <@t> yahoo.com (Sadushe Loxha)
Date: Sun Jun 12 07:21:51 2011
Subject: [Histonet] (no subject)
Message-ID: <432178.33520.qm@web36102.mail.mud.yahoo.com>

http://employmentsearchguide.com/glink.php
From Milton.Gomez <@t> nyumc.org  Sun Jun 12 12:20:17 2011
From: Milton.Gomez <@t> nyumc.org (Gomez, Milton)
Date: Sun Jun 12 12:20:23 2011
Subject: [Histonet] RE: Distribution of work for older generation of
	Histotechs
In-Reply-To: <4A53F9A1D7C2674FA4A6E650D703DDA5FB74FBAD@MSGWSDCPMB07.nyumc.org>
References: <4A53F9A1D7C2674FA4A6E650D703DDA5FB74FBAD@MSGWSDCPMB07.nyumc.org>
Message-ID: <4A53F9A1D7C2674FA4A6E650D703DDA5FCC7C7B7@MSGWSDCPMB07.nyumc.org>

Thank you all for your input on this issue.  I want to thank the older histotechs in the field for their support, wisdom, courage and dedication.  Special thanks to those who are in bench positions and still cranking the wheel!  I hope that we are not getting rid of them but accommodating their valued input for the improvement of our laboratories.  I hope we can encourage the younger histotechs to work with them as a team instead of competing with them.

MG

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gomez, Milton
Sent: Sunday, June 05, 2011 1:09 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Distribution of work for older generation of Histotechs

Hello Histonetters,

Is the distribution of work different for older histotechs vs. younger histotechs in your labs and why? Do they get special assignments or duties because of their growing wisdom and seniority?

Thanks in advance,

MG
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From kim.tournear <@t> yahoo.com  Sun Jun 12 12:30:50 2011
From: kim.tournear <@t> yahoo.com (Kim Tournear)
Date: Sun Jun 12 12:31:23 2011
Subject: [Histonet] general license question for Florida
Message-ID: <257237.74493.qm@web120215.mail.ne1.yahoo.com>

Hi everyone,
I was asked to post the following question from a technician/Supervisor outside of Florida:
?
If someone has their Florida State license for technician, and they?are HT certified (ASCP), but no BS degree, how many CE's (or is a BS required) are?needed in order to apply for the supervisor license? And is there an exam?
?
Thanks in advance for all replies......

~Kim~? ?
OU ROCKS!!!!
~Don't be afraid your life will end, 
be afraid it will never begin~
From rsrichmond <@t> gmail.com  Mon Jun 13 09:11:26 2011
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Mon Jun 13 09:11:31 2011
Subject: [Histonet] Formaldehyde in H2S's 12th RoC
Message-ID: 

The US Department of Health and Human Services (sometimes
affectionately known as H2S) has released the 12th Report on
Carcinogens (RoC). The report lists formaldehyde as a definite human
carcinogen. The regulatory repercussions of this aren't immediately
clear, but I suspect pathologists will see increasing pressure to
eliminate formaldehyde fixation. As the snake oil peddlers come out of
the woodwork, it's going to become increasingly important to remember
that there is no substitute for formaldehyde that will not greatly
change the way pathologists and histologists work.

A not entirely satisfactory New York Times account is at:
http://www.nytimes.com/2011/06/11/health/11cancer.html?hp

The report itself can be obtained in pieces in PDF form at:
http://ntp.niehs.nih.gov/?objectid=72016262-BDB7-CEBA-FA60E922B18C2540

The 12th RoC reports repeat the mantra "studies show" at Joyce
Brothers length, but do not really make clear what the facts are. They
do offer these references about mortality among embalmers and
pathologists. Does anyone on the list have access to these papers?

Hauptmann M, Lubin JH, Stewart PA, Hayes RB, Blair A. 2003. Mortality
from lymphohematopoietic
malignancies among workers in formaldehyde industries. J Natl Cancer
Inst 95(21): 1615-1623.
Hauptmann M, Lubin JH, Stewart PA, Hayes RB, Blair A. 2004. Mortality
from solid cancers among workers
in formaldehyde industries. Am J Epidemiol 159(12): 1117-1130.
Hauptmann M, Stewart PA, Lubin JH, Beane Freeman LE, Hornung RW,
Herrick RF, et al. 2009. Mortality
from lymphohematopoietic malignancies and brain cancer among embalmers
exposed to formaldehyde.
J Natl Cancer Inst 101(24): 1696-1708.

Posting this both to PATHO-L and to Histonet.

Bob Richmond
Samurai Pathologist
Knoxville TN

From GauchV <@t> mail.amc.edu  Mon Jun 13 09:40:08 2011
From: GauchV <@t> mail.amc.edu (Gauch, Vicki)
Date: Mon Jun 13 09:40:19 2011
Subject: [Histonet] Decal Solutions
Message-ID: 

Hi,
Does anyone know of any decal solutions that are compatible with IHC, ISH, FISH other than EDTA and solutions containing HCL ?  We have heard that some people use formic acid.  Also, what would be the decalcification times for tissue such as a bone marrow bx using these other solutions ?  Any help would be greatly appreciated...

Thanks,
Vicki Gauch
AMCH
Albany, NY



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From aj.taylor <@t> blueyonder.co.uk  Mon Jun 13 09:58:04 2011
From: aj.taylor <@t> blueyonder.co.uk (taylor alan)
Date: Mon Jun 13 09:58:08 2011
Subject: [Histonet] Looking for replacement drive belt for Leica Autostainer
	XL
Message-ID: 

Hello All.

We have recently aquired an 'oldish' AutostainerXL. It worked perfectly up
until the weekend, then the drive belt that lifts the robotic arm decided to
break! We are quite keen to find a replacement belt
as soon as possible.

I am asking if any of you Autostainer users have sources for replacement
drive belts other than Leica, as it is apparently no longer listed for
replacement parts on their web pages.

Any help and assistance would be gratefully received.

Regards

Alan

Alan Taylor
Microtechnical Services
71 Sweetbrier Lane
Heavitree
Exeter. Devon. EX1 3AJ
U.K.

Tel +44 (0) 1392 660132
Fax +44 (0) 1392 209990
From b-frederick <@t> northwestern.edu  Mon Jun 13 10:05:43 2011
From: b-frederick <@t> northwestern.edu (Bernice Frederick)
Date: Mon Jun 13 10:05:47 2011
Subject: [Histonet] Formaldehyde in H2S's 12th RoC
In-Reply-To: 
References: 
Message-ID: <003101cc29db$5d90a940$18b1fbc0$@northwestern.edu>

Well we still use Hematoxylin powder, Xylene ,Oil Red O, Beibrich scarlet
and Congo red and we haven't been stopped yet. 
I'd think embalmer would be well-fixed and preserved ,rather than have a
high mortality rate. Have the proven it's from formaldehyde? Whom knows what
the get from a dead body?
Bernice

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert
Richmond
Sent: Monday, June 13, 2011 9:11 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formaldehyde in H2S's 12th RoC

The US Department of Health and Human Services (sometimes affectionately
known as H2S) has released the 12th Report on Carcinogens (RoC). The report
lists formaldehyde as a definite human carcinogen. The regulatory
repercussions of this aren't immediately clear, but I suspect pathologists
will see increasing pressure to eliminate formaldehyde fixation. As the
snake oil peddlers come out of the woodwork, it's going to become
increasingly important to remember that there is no substitute for
formaldehyde that will not greatly change the way pathologists and
histologists work.

A not entirely satisfactory New York Times account is at:
http://www.nytimes.com/2011/06/11/health/11cancer.html?hp

The report itself can be obtained in pieces in PDF form at:
http://ntp.niehs.nih.gov/?objectid=72016262-BDB7-CEBA-FA60E922B18C2540

The 12th RoC reports repeat the mantra "studies show" at Joyce Brothers
length, but do not really make clear what the facts are. They do offer these
references about mortality among embalmers and pathologists. Does anyone on
the list have access to these papers?

Hauptmann M, Lubin JH, Stewart PA, Hayes RB, Blair A. 2003. Mortality from
lymphohematopoietic malignancies among workers in formaldehyde industries. J
Natl Cancer Inst 95(21): 1615-1623.
Hauptmann M, Lubin JH, Stewart PA, Hayes RB, Blair A. 2004. Mortality from
solid cancers among workers in formaldehyde industries. Am J Epidemiol
159(12): 1117-1130.
Hauptmann M, Stewart PA, Lubin JH, Beane Freeman LE, Hornung RW, Herrick RF,
et al. 2009. Mortality from lymphohematopoietic malignancies and brain
cancer among embalmers exposed to formaldehyde.
J Natl Cancer Inst 101(24): 1696-1708.

Posting this both to PATHO-L and to Histonet.

Bob Richmond
Samurai Pathologist
Knoxville TN

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From GaleL <@t> unionhospital.org  Mon Jun 13 10:16:00 2011
From: GaleL <@t> unionhospital.org (Gale Limron)
Date: Mon Jun 13 10:16:07 2011
Subject: [Histonet] formalin in OR/L&D
Message-ID: 

Hi,
 I know this topic has been discussed time-to-time but I was just approached with questions regarding the formalin cubes that are supplied to our OR and L&D departments.
 OR has a small room where 2.5 gal. cubes are kept under a hood. They set on a metal stand in a plastic tray. They would like to keep a layer of spill-absorbing material in the plastic tray to neutralize any drips or spills. Is this advisable?
 L&D has 5 gal. formalin cubes that are kept beside a sink in an area with no hood. If there are any drips or leaks they are washed down the drain. Any opinions on this system of storage?
 I would appreciate hearing how other places handle these types of storage issues.
Thank you,
Gale

Gale Limron CT, HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562



This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery.
From rjbuesa <@t> yahoo.com  Mon Jun 13 10:51:49 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Mon Jun 13 10:51:53 2011
Subject: [Histonet] formalin in OR/L&D
In-Reply-To: 
References: 
Message-ID: <343330.83882.qm@web65702.mail.ac4.yahoo.com>

Keeping formalin under a hood over a spill control pad is an acceptable practice, but having a larger formalin container?out of?a hood and dripping to a drain is totally unacceptable, more because it is an open container than because of a small dripping to the sewer.
Ren? J.

From: Gale Limron 
To: "histonet@lists.utsouthwestern.edu" 
Sent: Monday, June 13, 2011 11:16 AM
Subject: [Histonet] formalin in OR/L&D

Hi,
I know this topic has been discussed time-to-time but I was just approached with questions regarding the formalin cubes that are supplied to our OR and L&D departments.
OR has a small room where 2.5 gal. cubes are kept under a hood. They set on a metal stand in a plastic tray. They would like to keep a layer of spill-absorbing material in the plastic tray to neutralize any drips or spills. Is this advisable?
L&D has 5 gal. formalin cubes that are kept beside a sink in an area with no hood. If there are any drips or leaks they are washed down the drain. Any opinions on this system of storage?
I would appreciate hearing how other places handle these types of storage issues.
Thank you,
Gale

Gale Limron CT, HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562



This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From MSHERWOOD <@t> PARTNERS.ORG  Mon Jun 13 11:16:41 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Mon Jun 13 11:16:45 2011
Subject: [Histonet] Looking for replacement drive belt for Leica
	AutostainerXL
In-Reply-To: 
References: 
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB57F1@PHSXMB30.partners.org>

First, I would check with a Leica rep and see if he can help you.  We have
ordered many replacement parts for our Autostainer XL (refurbished). 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of taylor alan
Sent: Monday, June 13, 2011 10:58 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Looking for replacement drive belt for Leica AutostainerXL

Hello All.

We have recently aquired an 'oldish' AutostainerXL. It worked perfectly up
until the weekend, then the drive belt that lifts the robotic arm decided to
break! We are quite keen to find a replacement belt
as soon as possible.

I am asking if any of you Autostainer users have sources for replacement
drive belts other than Leica, as it is apparently no longer listed for
replacement parts on their web pages.

Any help and assistance would be gratefully received.

Regards

Alan

Alan Taylor
Microtechnical Services
71 Sweetbrier Lane
Heavitree
Exeter. Devon. EX1 3AJ
U.K.

Tel +44 (0) 1392 660132
Fax +44 (0) 1392 209990
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
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From sbreeden <@t> nmda.nmsu.edu  Mon Jun 13 13:33:56 2011
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Mon Jun 13 13:34:02 2011
Subject: [Histonet] OT: How do you call...
Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>

I've figured out how to fund  my approaching retirement!  A poll!  So...
is the correct term "Anatomical Pathology" or "Anatomic Pathology"?
Send $5.00 with your email  reply and I'll have the down payment for
that tropical island with internet.  Seriously - what is the consensus
and/or the correct usage?  Figure I better get all these Deep Questions
answered while I have the chance.  (P.S., I'm only kidding about the
$5.00 but I do take PayPal).

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

From lblazek <@t> digestivespecialists.com  Mon Jun 13 13:37:51 2011
From: lblazek <@t> digestivespecialists.com (Blazek, Linda)
Date: Mon Jun 13 13:38:04 2011
Subject: [Histonet] RE: OT: How do you call...
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBF65E9E5@IBMB7Exchange.digestivespecialists.com>

I vote for Anatomic Pathology.


Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 396-2623
Email: lblazek@digestivespecialists.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Monday, June 13, 2011 2:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] OT: How do you call...

I've figured out how to fund  my approaching retirement!  A poll!  So...
is the correct term "Anatomical Pathology" or "Anatomic Pathology"?
Send $5.00 with your email  reply and I'll have the down payment for
that tropical island with internet.  Seriously - what is the consensus
and/or the correct usage?  Figure I better get all these Deep Questions
answered while I have the chance.  (P.S., I'm only kidding about the
$5.00 but I do take PayPal).

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From lblazek <@t> digestivespecialists.com  Mon Jun 13 13:38:25 2011
From: lblazek <@t> digestivespecialists.com (Blazek, Linda)
Date: Mon Jun 13 13:38:31 2011
Subject: [Histonet] RE: OT: How do you call...
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBF65E9E6@IBMB7Exchange.digestivespecialists.com>

Oops....  I forgot to include the $5.00

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Monday, June 13, 2011 2:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] OT: How do you call...

I've figured out how to fund  my approaching retirement!  A poll!  So...
is the correct term "Anatomical Pathology" or "Anatomic Pathology"?
Send $5.00 with your email  reply and I'll have the down payment for
that tropical island with internet.  Seriously - what is the consensus
and/or the correct usage?  Figure I better get all these Deep Questions
answered while I have the chance.  (P.S., I'm only kidding about the
$5.00 but I do take PayPal).

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From mwhite <@t> mcleodhealth.org  Mon Jun 13 13:40:23 2011
From: mwhite <@t> mcleodhealth.org (mwhite@mcleodhealth.org)
Date: Mon Jun 13 13:40:27 2011
Subject: [Histonet] OT: How do you call...
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: 

Sally asks:   "I've figured out how to fund  my approaching retirement!  A
poll!  So...
is the correct term "Anatomical Pathology" or "Anatomic Pathology"?
Send $5.00 with your email  reply and I'll have the down payment for
that tropical island with internet.  Seriously - what is the consensus
and/or the correct usage?  Figure I better get all these Deep Questions
answered while I have the chance.  (P.S., I'm only kidding about the
$5.00 but I do take PayPal)."

Sally, our pathologists assert that the correct term is "Anatomic
Pathology".




Melanie S. White, MT(ASCP)
Laboratory Supervisor, Systems/Anatomic Pathology
McLeod Regional Medical Center
(843) 777-2072



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intended solely for the use of the addressee. If the reader of this 
message is not the intended recipient, you are hereby notified 
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From rsrichmond <@t> gmail.com  Mon Jun 13 13:44:30 2011
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Mon Jun 13 13:44:35 2011
Subject: [Histonet] Re: Formaldehyde in H2S's 12th RoC
Message-ID: 

Two people have now sent me PDF's of the three articles I listed in my
earlier post. Thanks!

I'll send these on to anyone who sends me their e-mail address.

Bob Richmond
Samurai Pathologist
Knoxville TN

From LRaff <@t> uropartners.com  Mon Jun 13 13:44:36 2011
From: LRaff <@t> uropartners.com (Lester Raff MD)
Date: Mon Jun 13 13:44:40 2011
Subject: [Histonet] OT: How do you call...
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: 

Board Certification (I checked certificate) reads "Anatomic and Clinical
Pathology"

Lester J. Raff, MD
Medical Director
UroPartners Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel 708.486.0076
Fax 708.492.0203

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Monday, June 13, 2011 1:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] OT: How do you call...

I've figured out how to fund  my approaching retirement!  A poll!  So...
is the correct term "Anatomical Pathology" or "Anatomic Pathology"?
Send $5.00 with your email  reply and I'll have the down payment for
that tropical island with internet.  Seriously - what is the consensus
and/or the correct usage?  Figure I better get all these Deep Questions
answered while I have the chance.  (P.S., I'm only kidding about the
$5.00 but I do take PayPal).

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From JWeems <@t> sjha.org  Mon Jun 13 13:45:39 2011
From: JWeems <@t> sjha.org (Weems, Joyce)
Date: Mon Jun 13 13:45:44 2011
Subject: [Histonet] RE: OT: How do you call...
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408210EA3F1@CHEXCMS10.one.ads.che.org>


Bob Richmond, where are you???? 


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Monday, June 13, 2011 14:34
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] OT: How do you call...

I've figured out how to fund  my approaching retirement!  A poll!  So...
is the correct term "Anatomical Pathology" or "Anatomic Pathology"?
Send $5.00 with your email  reply and I'll have the down payment for that tropical island with internet.  Seriously - what is the consensus and/or the correct usage?  Figure I better get all these Deep Questions answered while I have the chance.  (P.S., I'm only kidding about the $5.00 but I do take PayPal).

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This e-mail, including any attachments is the 
property of Catholic Health East and is intended 
for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are 
not the intended recipient, please delete this message, and 
reply to the sender regarding the error in a separate email.


From shive003 <@t> umn.edu  Mon Jun 13 13:51:37 2011
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Mon Jun 13 13:51:41 2011
Subject: [Histonet] OT: How do you call...
References: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: 

When taking coursework eons ago, one of my professors addressed this very 
issue.  He said that '-ic' and '-al' were BOTH adjective suffixes... and 
there was no need to include both at the end of a noun to turn it into an 
adjective (redundancy).

So 'anatomic' it is.  And it follows that another correct word spelling is 
'pathologic', not 'pathological'.... though the latter is commonly used.

Jan Shivers
UMN

----- Original Message ----- 
From: "Breeden, Sara" 
To: 
Sent: Monday, June 13, 2011 1:33 PM
Subject: [Histonet] OT: How do you call...


I've figured out how to fund  my approaching retirement!  A poll!  So...
is the correct term "Anatomical Pathology" or "Anatomic Pathology"?
Send $5.00 with your email  reply and I'll have the down payment for
that tropical island with internet.  Seriously - what is the consensus
and/or the correct usage?  Figure I better get all these Deep Questions
answered while I have the chance.  (P.S., I'm only kidding about the
$5.00 but I do take PayPal).



Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From leiker <@t> buffalo.edu  Mon Jun 13 14:02:17 2011
From: leiker <@t> buffalo.edu (Merced Leiker)
Date: Mon Jun 13 14:02:21 2011
Subject: [Histonet] OT: How do you call...
In-Reply-To: 
References: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
	
Message-ID: <4DF65EB9.9030707@buffalo.edu>

I'm guessing, then, that "anatomal" and "pathalogal" aren't words...?



On 6/13/2011 2:51 PM, Jan Shivers wrote:
> When taking coursework eons ago, one of my professors addressed this 
> very issue.  He said that '-ic' and '-al' were BOTH adjective 
> suffixes... and there was no need to include both at the end of a noun 
> to turn it into an adjective (redundancy).
>
> So 'anatomic' it is.  And it follows that another correct word 
> spelling is 'pathologic', not 'pathological'.... though the latter is 
> commonly used.
>
> Jan Shivers
> UMN
>
> ----- Original Message ----- From: "Breeden, Sara" 
> 
> To: 
> Sent: Monday, June 13, 2011 1:33 PM
> Subject: [Histonet] OT: How do you call...
>
>
> I've figured out how to fund  my approaching retirement!  A poll!  So...
> is the correct term "Anatomical Pathology" or "Anatomic Pathology"?
> Send $5.00 with your email  reply and I'll have the down payment for
> that tropical island with internet.  Seriously - what is the consensus
> and/or the correct usage?  Figure I better get all these Deep Questions
> answered while I have the chance.  (P.S., I'm only kidding about the
> $5.00 but I do take PayPal).
>
>
>
> Sally Breeden, HT(ASCP)
>
> New Mexico Department of Agriculture
>
> Veterinary Diagnostic Services
>
> 1101 Camino de Salud NE
>
> Albuquerque, NM  87102
>
> 505-383-9278 (Histology Lab)
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


From sbreeden <@t> nmda.nmsu.edu  Mon Jun 13 14:03:25 2011
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Mon Jun 13 14:03:29 2011
Subject: [Histonet] Anatomicalalal
Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF747@nmdamailsvr.nmda.ad.nmsu.edu>

I just LOVE it when I'm right - and it happens so rarely!  The consensus
of ten responders so far is ANATOMIC.  This just makes sense to me - we
don't call them Diagnostical Pathologists, do we?  Nah.  Keep those
correct answers coming my way - I needed the confirmation!  And I have
$50 toward the rowboat that'll take me to that island.  Can you tell I
need the diversion today?

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

From sbreeden <@t> nmda.nmsu.edu  Mon Jun 13 14:05:33 2011
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Mon Jun 13 14:05:37 2011
Subject: [Histonet] OT: How do you call...
In-Reply-To: <4DF65EB9.9030707@buffalo.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
	<4DF65EB9.9030707@buffalo.edu>
Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF749@nmdamailsvr.nmda.ad.nmsu.edu>

Huh???

From shive003 <@t> umn.edu  Mon Jun 13 14:08:16 2011
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Mon Jun 13 14:08:20 2011
Subject: [Histonet] OT: How do you call...
References: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
	<4DF65EB9.9030707@buffalo.edu>
Message-ID: <9151AD985A0C43DEB5115C990916C857@auxs.umn.edu>

I guess when given a choice long ago, English wordsmiths chose the ending 
which sounded better and put it into use.  Just like we say 'psychic' and 
not 'psychal'... but of course there are still people who say 'psychical'!

But again, I'm only repeating what I was taught re: anatomic vs anatomical. 
Language does change over time - just check out what's included in 
dictionaries these days!
Jan

----- Original Message ----- 
From: "Merced Leiker" 
To: 
Sent: Monday, June 13, 2011 2:02 PM
Subject: Re: [Histonet] OT: How do you call...


> I'm guessing, then, that "anatomal" and "pathalogal" aren't words...?
>
>
>
> On 6/13/2011 2:51 PM, Jan Shivers wrote:
>> When taking coursework eons ago, one of my professors addressed this very 
>> issue.  He said that '-ic' and '-al' were BOTH adjective suffixes... and 
>> there was no need to include both at the end of a noun to turn it into an 
>> adjective (redundancy).
>>
>> So 'anatomic' it is.  And it follows that another correct word spelling 
>> is 'pathologic', not 'pathological'.... though the latter is commonly 
>> used.
>>
>> Jan Shivers
>> UMN
>>
>> ----- Original Message ----- From: "Breeden, Sara" 
>> 
>> To: 
>> Sent: Monday, June 13, 2011 1:33 PM
>> Subject: [Histonet] OT: How do you call...
>>
>>
>> I've figured out how to fund  my approaching retirement!  A poll!  So...
>> is the correct term "Anatomical Pathology" or "Anatomic Pathology"?
>> Send $5.00 with your email  reply and I'll have the down payment for
>> that tropical island with internet.  Seriously - what is the consensus
>> and/or the correct usage?  Figure I better get all these Deep Questions
>> answered while I have the chance.  (P.S., I'm only kidding about the
>> $5.00 but I do take PayPal).
>>
>>
>>
>> Sally Breeden, HT(ASCP)
>>
>> New Mexico Department of Agriculture
>>
>> Veterinary Diagnostic Services
>>
>> 1101 Camino de Salud NE
>>
>> Albuquerque, NM  87102
>>
>> 505-383-9278 (Histology Lab)
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From baderbo <@t> gmail.com  Mon Jun 13 14:10:08 2011
From: baderbo <@t> gmail.com (Bader Siddiki)
Date: Mon Jun 13 14:10:13 2011
Subject: [Histonet] Light + IF microscope
Message-ID: 

Hello Histonetters
I need your professional advice.
We are a small IHC and IF company looking for a good microscope.
We have investigated in Nikon;s they have 50i and 55i microscope that one
can use for IHC and IF with proper attachment, but pretty steep price.
Do you have any recommendation for a good quality microscope w/ IF
attachments.
May be one of you have a microscope like this and want to sell at a
reasonable price.
Your expert advice and recommendation will be appreciated.
Thank you.
Bader


Bader B Siddiki, PhD
Executive director,
Research and development
ImmunoBioScience Corp. (IBSC)
Phone: + 1 425 367 4601
Fax: + 1 425 367 4817
cell (mobile) phone: + 1 425 314 0199
e-mail address: BaderBo@Gmail.com
Web site: www.ImmunoBioScience.Com
Marketing: phone: + 1 650 343 IBSC (4272)
                E-mail: AnitaIBSC@Aol.com
From akbitting <@t> geisinger.edu  Mon Jun 13 14:23:14 2011
From: akbitting <@t> geisinger.edu (Angela Bitting)
Date: Mon Jun 13 14:23:23 2011
Subject: [Histonet] Anatomicalalal
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF747@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF747@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <4DF62B62.2B7F.00C9.1@geisinger.edu>

Shall we all debate the pronunciation of Periodic acid next ?!?! I've heard it 3 different ways so far in my career.

>>> "Breeden, Sara"  6/13/2011 3:03 PM >>>
I just LOVE it when I'm right - and it happens so rarely!  The consensus
of ten responders so far is ANATOMIC.  This just makes sense to me - we
don't call them Diagnostical Pathologists, do we?  Nah.  Keep those
correct answers coming my way - I needed the confirmation!  And I have
$50 toward the rowboat that'll take me to that island.  Can you tell I
need the diversion today?



Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
From sbreeden <@t> nmda.nmsu.edu  Mon Jun 13 14:25:44 2011
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Mon Jun 13 14:25:47 2011
Subject: [Histonet] Anatomicalalal
In-Reply-To: <4DF62B62.2B7F.00C9.1@geisinger.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF747@nmdamailsvr.nmda.ad.nmsu.edu>
	<4DF62B62.2B7F.00C9.1@geisinger.edu>
Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF74A@nmdamailsvr.nmda.ad.nmsu.edu>

Okay - more money for my retirement fund!  Is it Periodic Acid?
Periodic Table of the Elements?  Periodical Acid? Periodical Table of
the Elements?  Is "Time" magazine a periodic or a periodical?
Ooohhh...this is fun.  However, I've probably just about worn out my
welcome in the OT portion of Histonet, so I should go back into hiding.

From lblazek <@t> digestivespecialists.com  Mon Jun 13 14:29:05 2011
From: lblazek <@t> digestivespecialists.com (Blazek, Linda)
Date: Mon Jun 13 14:29:10 2011
Subject: [Histonet] Anatomicalalal
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF74A@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF747@nmdamailsvr.nmda.ad.nmsu.edu>
	<4DF62B62.2B7F.00C9.1@geisinger.edu>
	<4D14F0FC9316DD41972D5F03C070908B051DF74A@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBF65E9E8@IBMB7Exchange.digestivespecialists.com>

Don't go into hiding!  I was beginning to worry about you.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Monday, June 13, 2011 3:26 PM
To: Angela Bitting; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Anatomicalalal

Okay - more money for my retirement fund!  Is it Periodic Acid?
Periodic Table of the Elements?  Periodical Acid? Periodical Table of
the Elements?  Is "Time" magazine a periodic or a periodical?
Ooohhh...this is fun.  However, I've probably just about worn out my
welcome in the OT portion of Histonet, so I should go back into hiding.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From PMonfils <@t> Lifespan.org  Mon Jun 13 14:30:17 2011
From: PMonfils <@t> Lifespan.org (Monfils, Paul)
Date: Mon Jun 13 14:30:24 2011
Subject: [Histonet] Anatomicalalal
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF74A@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF747@nmdamailsvr.nmda.ad.nmsu.edu><4DF62B62.2B7F.00C9.1@geisinger.edu>
	<4D14F0FC9316DD41972D5F03C070908B051DF74A@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <4EBFF65383B74D49995298C4976D1D5E083E6B02@LSRIEXCH1.lsmaster.lifespan.org>

Actually, it's period'-ic for the table - it is arranged in periods.
But it is per-io'dic for the chemical, which has nothing to do with
periods, but everything to do with iodine!  :-)



From talulahgosh <@t> gmail.com  Mon Jun 13 14:46:08 2011
From: talulahgosh <@t> gmail.com (Emily Sours)
Date: Mon Jun 13 14:46:13 2011
Subject: [Histonet] Light + IF microscope
In-Reply-To: 
References: 
Message-ID: 

Nikon is expensive, but they are very friendly and will fix little things
for you for free (not that they'll tell you that upfront).  Our Nikon
representative is always coming by if we have even the slightest problem and
is willing to figure out weird problems that come up.  Also, they always
check the alignment on our FL bulb because I really hate doing it and I can
never get it right (though it seems so damn easy when the guy is showing me
how to do it!).
We have a Nikon E600 that is decades old and still in perfect condition.
This scope is actually featured on the microscope museum page Nikon runs, I
was very surprised to see it there!
You should try talking to the representative to see if you can get some sort
of deal.  They are very friendly and very willing to work with you.  I would
tell you to talk to Adam Henry but he works for Pennsylvania only.  He's
excellent!
You might be able to get something used from them, you never know.
Personally, I'd pay for something new, no matter how expensive (of course,
who isn't working under a grant right now? me! so I shouldn't really talk
about spending loads of money!).  Nikon is a good brand and definitely worth
the cost.
Also, pay for the APO objectives, it's worth it.  You can tell the
difference.  I have no idea what APO stands for, but they work better than
regular ones.

Emily

A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Mon, Jun 13, 2011 at 3:10 PM, Bader Siddiki  wrote:

> Hello Histonetters
> I need your professional advice.
> We are a small IHC and IF company looking for a good microscope.
> We have investigated in Nikon;s they have 50i and 55i microscope that one
> can use for IHC and IF with proper attachment, but pretty steep price.
> Do you have any recommendation for a good quality microscope w/ IF
> attachments.
> May be one of you have a microscope like this and want to sell at a
> reasonable price.
> Your expert advice and recommendation will be appreciated.
> Thank you.
> Bader
>
>
> Bader B Siddiki, PhD
> Executive director,
> Research and development
> ImmunoBioScience Corp. (IBSC)
> Phone: + 1 425 367 4601
> Fax: + 1 425 367 4817
> cell (mobile) phone: + 1 425 314 0199
> e-mail address: BaderBo@Gmail.com
> Web site: www.ImmunoBioScience.Com
> Marketing: phone: + 1 650 343 IBSC (4272)
>                E-mail: AnitaIBSC@Aol.com
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From Ronald.Houston <@t> nationwidechildrens.org  Mon Jun 13 14:52:16 2011
From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald)
Date: Mon Jun 13 14:52:25 2011
Subject: [Histonet] Light + IF microscope
In-Reply-To: 
References: 
	
Message-ID: 

APO stands for apochromatic, indicating an objective with the highest degree of correction for spherical and chromatic aberrations

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Monday, June 13, 2011 3:46 PM
To: Bader Siddiki; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Light + IF microscope

Nikon is expensive, but they are very friendly and will fix little things
for you for free (not that they'll tell you that upfront).  Our Nikon
representative is always coming by if we have even the slightest problem and
is willing to figure out weird problems that come up.  Also, they always
check the alignment on our FL bulb because I really hate doing it and I can
never get it right (though it seems so damn easy when the guy is showing me
how to do it!).
We have a Nikon E600 that is decades old and still in perfect condition.
This scope is actually featured on the microscope museum page Nikon runs, I
was very surprised to see it there!
You should try talking to the representative to see if you can get some sort
of deal.  They are very friendly and very willing to work with you.  I would
tell you to talk to Adam Henry but he works for Pennsylvania only.  He's
excellent!
You might be able to get something used from them, you never know.
Personally, I'd pay for something new, no matter how expensive (of course,
who isn't working under a grant right now? me! so I shouldn't really talk
about spending loads of money!).  Nikon is a good brand and definitely worth
the cost.
Also, pay for the APO objectives, it's worth it.  You can tell the
difference.  I have no idea what APO stands for, but they work better than
regular ones.

Emily

A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Mon, Jun 13, 2011 at 3:10 PM, Bader Siddiki  wrote:

> Hello Histonetters
> I need your professional advice.
> We are a small IHC and IF company looking for a good microscope.
> We have investigated in Nikon;s they have 50i and 55i microscope that one
> can use for IHC and IF with proper attachment, but pretty steep price.
> Do you have any recommendation for a good quality microscope w/ IF
> attachments.
> May be one of you have a microscope like this and want to sell at a
> reasonable price.
> Your expert advice and recommendation will be appreciated.
> Thank you.
> Bader
>
>
> Bader B Siddiki, PhD
> Executive director,
> Research and development
> ImmunoBioScience Corp. (IBSC)
> Phone: + 1 425 367 4601
> Fax: + 1 425 367 4817
> cell (mobile) phone: + 1 425 314 0199
> e-mail address: BaderBo@Gmail.com
> Web site: www.ImmunoBioScience.Com
> Marketing: phone: + 1 650 343 IBSC (4272)
>                E-mail: AnitaIBSC@Aol.com
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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From thomas6093 <@t> yahoo.com  Mon Jun 13 15:08:24 2011
From: thomas6093 <@t> yahoo.com (Thomas Huynh)
Date: Mon Jun 13 15:08:30 2011
Subject: [Histonet] Re: Decal Soloutions
Message-ID: <230873.97732.qm@web80407.mail.mud.yahoo.com>

Hi Vicki

We use 10% Formic Acid to decal our bone marrow core biopsies( Microwave 
decal-2.5 hours), then process as normal bx run on the processor.

Thomas Huynh
713-745-4759




________________________________
From: "histonet-request@lists.utsouthwestern.edu" 

To: histonet@lists.utsouthwestern.edu
Sent: Mon, June 13, 2011 12:01:37 PM
Subject: Histonet Digest, Vol 91, Issue 17

Send Histonet mailing list submissions to
??? histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
??? histonet-request@lists.utsouthwestern.edu

You can reach the person managing the list at
??? histonet-owner@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

? 1. RE: Distribution of work for older generation of??? Histotechs
? ? ? (Gomez, Milton)
? 2. general license question for Florida (Kim Tournear)
? 3. Formaldehyde in H2S's 12th RoC (Robert Richmond)
? 4. Decal Solutions (Gauch, Vicki)
? 5. Looking for replacement drive belt for Leica Autostainer??? XL
? ? ? (taylor alan)
? 6. RE: Formaldehyde in H2S's 12th RoC (Bernice Frederick)
? 7. formalin in OR/L&D (Gale Limron)
? 8. Re: formalin in OR/L&D (Rene J Buesa)
? 9. RE: Looking for replacement drive belt for Leica
? ? ? AutostainerXL (Sherwood, Margaret )


----------------------------------------------------------------------

Message: 1
Date: Sun, 12 Jun 2011 13:20:17 -0400
From: "Gomez, Milton" 
Subject: [Histonet] RE: Distribution of work for older generation of
??? Histotechs
To: "Gomez, Milton" ,
??? "'histonet@lists.utsouthwestern.edu'"
??? 
Message-ID:
??? <4A53F9A1D7C2674FA4A6E650D703DDA5FCC7C7B7@MSGWSDCPMB07.nyumc.org>
Content-Type: text/plain; charset="us-ascii"

Thank you all for your input on this issue.? I want to thank the older 
histotechs in the field for their support, wisdom, courage and dedication.? 
Special thanks to those who are in bench positions and still cranking the 
wheel!? I hope that we are not getting rid of them but accommodating their 
valued input for the improvement of our laboratories.? I hope we can encourage 
the younger histotechs to work with them as a team instead of competing with 
them.

MG

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gomez, Milton
Sent: Sunday, June 05, 2011 1:09 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Distribution of work for older generation of Histotechs

Hello Histonetters,

Is the distribution of work different for older histotechs vs. younger 
histotechs in your labs and why? Do they get special assignments or duties 
because of their growing wisdom and seniority?

Thanks in advance,

MG
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------------------------------

Message: 2
Date: Sun, 12 Jun 2011 10:30:50 -0700 (PDT)
From: Kim Tournear 
Subject: [Histonet] general license question for Florida
To: Histonet 
Message-ID: <257237.74493.qm@web120215.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi everyone,
I was asked to post the following question from a technician/Supervisor outside 
of Florida:
?
If someone has their Florida State license for technician, and they?are HT 
certified (ASCP), but no BS degree, how many CE's (or is a BS required) 
are?needed in order to apply for the supervisor license? And is there an exam?
?
Thanks in advance for all replies......

~Kim~? ?
OU ROCKS!!!!
~Don't be afraid your life will end, 
be afraid it will never begin~

------------------------------

Message: 3
Date: Mon, 13 Jun 2011 10:11:26 -0400
From: Robert Richmond 
Subject: [Histonet] Formaldehyde in H2S's 12th RoC
To: "Histonet@lists.utsouthwestern.edu"
??? 
Message-ID: 
Content-Type: text/plain; charset=ISO-8859-1

The US Department of Health and Human Services (sometimes
affectionately known as H2S) has released the 12th Report on
Carcinogens (RoC). The report lists formaldehyde as a definite human
carcinogen. The regulatory repercussions of this aren't immediately
clear, but I suspect pathologists will see increasing pressure to
eliminate formaldehyde fixation. As the snake oil peddlers come out of
the woodwork, it's going to become increasingly important to remember
that there is no substitute for formaldehyde that will not greatly
change the way pathologists and histologists work.

A not entirely satisfactory New York Times account is at:
http://www.nytimes.com/2011/06/11/health/11cancer.html?hp

The report itself can be obtained in pieces in PDF form at:
http://ntp.niehs.nih.gov/?objectid=72016262-BDB7-CEBA-FA60E922B18C2540

The 12th RoC reports repeat the mantra "studies show" at Joyce
Brothers length, but do not really make clear what the facts are. They
do offer these references about mortality among embalmers and
pathologists. Does anyone on the list have access to these papers?

Hauptmann M, Lubin JH, Stewart PA, Hayes RB, Blair A. 2003. Mortality
from lymphohematopoietic
malignancies among workers in formaldehyde industries. J Natl Cancer
Inst 95(21): 1615-1623.
Hauptmann M, Lubin JH, Stewart PA, Hayes RB, Blair A. 2004. Mortality
from solid cancers among workers
in formaldehyde industries. Am J Epidemiol 159(12): 1117-1130.
Hauptmann M, Stewart PA, Lubin JH, Beane Freeman LE, Hornung RW,
Herrick RF, et al. 2009. Mortality
from lymphohematopoietic malignancies and brain cancer among embalmers
exposed to formaldehyde.
J Natl Cancer Inst 101(24): 1696-1708.

Posting this both to PATHO-L and to Histonet.

Bob Richmond
Samurai Pathologist
Knoxville TN



------------------------------

Message: 4
Date: Mon, 13 Jun 2011 10:40:08 -0400
From: "Gauch, Vicki" 
Subject: [Histonet] Decal Solutions
To: "histonet@lists.utsouthwestern.edu"
??? 
Message-ID:
??? 
??? 
Content-Type: text/plain;??? charset="us-ascii"

Hi,
Does anyone know of any decal solutions that are compatible with IHC, ISH, FISH 
other than EDTA and solutions containing HCL ?? We have heard that some people 
use formic acid.? Also, what would be the decalcification times for tissue such 
as a bone marrow bx using these other solutions ?? Any help would be greatly 
appreciated...

Thanks,
Vicki Gauch
AMCH
Albany, NY



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Message: 5
Date: Mon, 13 Jun 2011 15:58:04 +0100
From: taylor alan 
Subject: [Histonet] Looking for replacement drive belt for Leica
??? Autostainer??? XL
To: Histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset=ISO-8859-1

Hello All.

We have recently aquired an 'oldish' AutostainerXL. It worked perfectly up
until the weekend, then the drive belt that lifts the robotic arm decided to
break! We are quite keen to find a replacement belt
as soon as possible.

I am asking if any of you Autostainer users have sources for replacement
drive belts other than Leica, as it is apparently no longer listed for
replacement parts on their web pages.

Any help and assistance would be gratefully received.

Regards

Alan

Alan Taylor
Microtechnical Services
71 Sweetbrier Lane
Heavitree
Exeter. Devon. EX1 3AJ
U.K.

Tel +44 (0) 1392 660132
Fax +44 (0) 1392 209990


------------------------------

Message: 6
Date: Mon, 13 Jun 2011 10:05:43 -0500
From: "Bernice Frederick" 
Subject: RE: [Histonet] Formaldehyde in H2S's 12th RoC
To: "'Robert Richmond'" ,
??? "'Histonet@lists.utsouthwestern.edu'"
??? 
Message-ID: <003101cc29db$5d90a940$18b1fbc0$@northwestern.edu>
Content-Type: text/plain;??? charset="us-ascii"

Well we still use Hematoxylin powder, Xylene ,Oil Red O, Beibrich scarlet
and Congo red and we haven't been stopped yet. 
I'd think embalmer would be well-fixed and preserved ,rather than have a
high mortality rate. Have the proven it's from formaldehyde? Whom knows what
the get from a dead body?
Bernice

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert
Richmond
Sent: Monday, June 13, 2011 9:11 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formaldehyde in H2S's 12th RoC

The US Department of Health and Human Services (sometimes affectionately
known as H2S) has released the 12th Report on Carcinogens (RoC). The report
lists formaldehyde as a definite human carcinogen. The regulatory
repercussions of this aren't immediately clear, but I suspect pathologists
will see increasing pressure to eliminate formaldehyde fixation. As the
snake oil peddlers come out of the woodwork, it's going to become
increasingly important to remember that there is no substitute for
formaldehyde that will not greatly change the way pathologists and
histologists work.

A not entirely satisfactory New York Times account is at:
http://www.nytimes.com/2011/06/11/health/11cancer.html?hp

The report itself can be obtained in pieces in PDF form at:
http://ntp.niehs.nih.gov/?objectid=72016262-BDB7-CEBA-FA60E922B18C2540

The 12th RoC reports repeat the mantra "studies show" at Joyce Brothers
length, but do not really make clear what the facts are. They do offer these
references about mortality among embalmers and pathologists. Does anyone on
the list have access to these papers?

Hauptmann M, Lubin JH, Stewart PA, Hayes RB, Blair A. 2003. Mortality from
lymphohematopoietic malignancies among workers in formaldehyde industries. J
Natl Cancer Inst 95(21): 1615-1623.
Hauptmann M, Lubin JH, Stewart PA, Hayes RB, Blair A. 2004. Mortality from
solid cancers among workers in formaldehyde industries. Am J Epidemiol
159(12): 1117-1130.
Hauptmann M, Stewart PA, Lubin JH, Beane Freeman LE, Hornung RW, Herrick RF,
et al. 2009. Mortality from lymphohematopoietic malignancies and brain
cancer among embalmers exposed to formaldehyde.
J Natl Cancer Inst 101(24): 1696-1708.

Posting this both to PATHO-L and to Histonet.

Bob Richmond
Samurai Pathologist
Knoxville TN

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 7
Date: Mon, 13 Jun 2011 11:16:00 -0400
From: Gale Limron 
Subject: [Histonet] formalin in OR/L&D
To: "histonet@lists.utsouthwestern.edu"
??? 
Message-ID:
??? 
Content-Type: text/plain; charset="us-ascii"

Hi,
I know this topic has been discussed time-to-time but I was just approached with 
questions regarding the formalin cubes that are supplied to our OR and L&D 
departments.
OR has a small room where 2.5 gal. cubes are kept under a hood. They set on a 
metal stand in a plastic tray. They would like to keep a layer of 
spill-absorbing material in the plastic tray to neutralize any drips or spills. 
Is this advisable?
L&D has 5 gal. formalin cubes that are kept beside a sink in an area with no 
hood. If there are any drips or leaks they are washed down the drain. Any 
opinions on this system of storage?
I would appreciate hearing how other places handle these types of storage 
issues.
Thank you,
Gale

Gale Limron CT, HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562



This e-mail is intended only for the person or entity to which it is addressed 
and may contain information that is privileged, confidential or otherwise 
protected from disclosure. Dissemination, distribution or copying of this e-mail 
or the information herein by anyone other than the intended recipient, or an 
employee or agent responsible for delivering the message to the intended 
recipient, is prohibited. If you received this message in error, please delete 
without copying and kindly e-mail a reply to inform us of the mistake in 
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------------------------------

Message: 8
Date: Mon, 13 Jun 2011 08:51:49 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] formalin in OR/L&D
To: Gale Limron ,
??? "histonet@lists.utsouthwestern.edu"
??? 
Message-ID: <343330.83882.qm@web65702.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Keeping formalin under a hood over a spill control pad is an acceptable 
practice, but having a larger formalin container?out of?a hood and dripping to a 
drain is totally unacceptable, more because it is an open container than because 
of a small dripping to the sewer.
Ren? J.

From: Gale Limron 
To: "histonet@lists.utsouthwestern.edu" 
Sent: Monday, June 13, 2011 11:16 AM
Subject: [Histonet] formalin in OR/L&D

Hi,
I know this topic has been discussed time-to-time but I was just approached with 
questions regarding the formalin cubes that are supplied to our OR and L&D 
departments.
OR has a small room where 2.5 gal. cubes are kept under a hood. They set on a 
metal stand in a plastic tray. They would like to keep a layer of 
spill-absorbing material in the plastic tray to neutralize any drips or spills. 
Is this advisable?
L&D has 5 gal. formalin cubes that are kept beside a sink in an area with no 
hood. If there are any drips or leaks they are washed down the drain. Any 
opinions on this system of storage?
I would appreciate hearing how other places handle these types of storage 
issues.
Thank you,
Gale

Gale Limron CT, HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562



This e-mail is intended only for the person or entity to which it is addressed 
and may contain information that is privileged, confidential or otherwise 
protected from disclosure. Dissemination, distribution or copying of this e-mail 
or the information herein by anyone other than the intended recipient, or an 
employee or agent responsible for delivering the message to the intended 
recipient, is prohibited. If you received this message in error, please delete 
without copying and kindly e-mail a reply to inform us of the mistake in 
delivery.
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 9
Date: Mon, 13 Jun 2011 12:16:41 -0400
From: "Sherwood, Margaret " 
Subject: RE: [Histonet] Looking for replacement drive belt for Leica
??? AutostainerXL
To: "taylor alan" ,
??? 
Message-ID:
??? <073AE2BEA1C2BA4A8837AB6C4B943D9708DB57F1@PHSXMB30.partners.org>
Content-Type: text/plain; charset="us-ascii"

First, I would check with a Leica rep and see if he can help you.? We have
ordered many replacement parts for our Autostainer XL (refurbished). 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of taylor alan
Sent: Monday, June 13, 2011 10:58 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Looking for replacement drive belt for Leica AutostainerXL

Hello All.

We have recently aquired an 'oldish' AutostainerXL. It worked perfectly up
until the weekend, then the drive belt that lifts the robotic arm decided to
break! We are quite keen to find a replacement belt
as soon as possible.

I am asking if any of you Autostainer users have sources for replacement
drive belts other than Leica, as it is apparently no longer listed for
replacement parts on their web pages.

Any help and assistance would be gratefully received.

Regards

Alan

Alan Taylor
Microtechnical Services
71 Sweetbrier Lane
Heavitree
Exeter. Devon. EX1 3AJ
U.K.

Tel +44 (0) 1392 660132
Fax +44 (0) 1392 209990
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
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------------------------------

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End of Histonet Digest, Vol 91, Issue 17
****************************************
From billodonnell <@t> catholichealth.net  Mon Jun 13 15:28:28 2011
From: billodonnell <@t> catholichealth.net (O'Donnell, Bill)
Date: Mon Jun 13 15:30:05 2011
Subject: [Histonet] RE: OT: How do you call...
In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EBF65E9E5@IBMB7Exchange.digestivespecialists.com>
References: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
	<5A2BD13465E061429D6455C8D6B40E390EBF65E9E5@IBMB7Exchange.digestivespecialists.com>
Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395084D45@chimsx08.CHI.catholichealth.net>

Anatomic is correct. (The checks in the mail) 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Monday, June 13, 2011 1:38 PM
To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: OT: How do you call...

I vote for Anatomic Pathology.


Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 396-2623
Email: lblazek@digestivespecialists.com



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Monday, June 13, 2011 2:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] OT: How do you call...

I've figured out how to fund  my approaching retirement!  A poll!  So...
is the correct term "Anatomical Pathology" or "Anatomic Pathology"?
Send $5.00 with your email  reply and I'll have the down payment for
that tropical island with internet.  Seriously - what is the consensus
and/or the correct usage?  Figure I better get all these Deep Questions
answered while I have the chance.  (P.S., I'm only kidding about the
$5.00 but I do take PayPal).

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From sgoebel <@t> mirnarx.com  Mon Jun 13 15:32:29 2011
From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com)
Date: Mon Jun 13 15:32:33 2011
Subject: [Histonet] Vimentin
Message-ID: 

Does anyone have a picture of a positive control Vimentin on human
tonsil?  I'm getting some arguments...

 

Sarah Goebel-Dysart, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

From igor.deyneko <@t> gmail.com  Mon Jun 13 15:37:17 2011
From: igor.deyneko <@t> gmail.com (Igor Deyneko)
Date: Mon Jun 13 15:37:19 2011
Subject: [Histonet] Reticulin Stain(Gomori)
Message-ID: 

Dear Histonetters!
I was wondering if anyone has a good working Gomori stain protocol for
Reticulin fibers. i understand that it's a pretty routine procedure; however
no one in my lab has ever done it, so I'm wondering if there is a
ready-to-use kit already or is it all from scratch. I'm trying to stain
liver, spleens and bone marrows.Thank you very much in advance for any
advise/help/protocols.
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA 02139
From bakevictoria <@t> gmail.com  Mon Jun 13 15:43:40 2011
From: bakevictoria <@t> gmail.com (Victoria Baker)
Date: Mon Jun 13 15:43:43 2011
Subject: [Histonet] Reticulin Stain(Gomori)
In-Reply-To: 
References: 
Message-ID: 

Polyscientific of Bayshore NY has a very simple kit.  You can find them on
line.
Vikki
On Jun 13, 2011 4:37 PM, "Igor Deyneko"  wrote:
> Dear Histonetters!
> I was wondering if anyone has a good working Gomori stain protocol for
> Reticulin fibers. i understand that it's a pretty routine procedure;
however
> no one in my lab has ever done it, so I'm wondering if there is a
> ready-to-use kit already or is it all from scratch. I'm trying to stain
> liver, spleens and bone marrows.Thank you very much in advance for any
> advise/help/protocols.
> Igor Deyneko
> Infinity Pharmaceuticals
> Cambridge, MA 02139
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From rjbuesa <@t> yahoo.com  Mon Jun 13 15:46:46 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Mon Jun 13 15:46:50 2011
Subject: [Histonet] OT: How do you call...
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <721329.20138.qm@web65710.mail.ac4.yahoo.com>

Neither, it is "Pathological Anatomy" because the anatomy becomes pathological and that is what the pathologists study?using our slides.
Ren? J.
I've figured out how to fund? my approaching retirement!? A poll!? So...
is the correct term "Anatomical Pathology" or "Anatomic Pathology"?
Send $5.00 with your email? reply and I'll have the down payment for
that tropical island with internet.? Seriously - what is the consensus
and/or the correct usage?? Figure I better get all these Deep Questions
answered while I have the chance.? (P.S., I'm only kidding about the
$5.00 but I do take PayPal).



Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM? 87102

505-383-9278 (Histology Lab)



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From: "Breeden, Sara" 
To: histonet@lists.utsouthwestern.edu
Sent: Monday, June 13, 2011 2:33 PM
Subject: [Histonet] OT: How do you call...
From rsrichmond <@t> gmail.com  Mon Jun 13 15:54:41 2011
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Mon Jun 13 15:54:48 2011
Subject: [Histonet] Re: Anatomicalalal
Message-ID: 

Well, I've been a pathologist for embarrassingly close to fifty years,
and I've never heard anything but "anatomic", and that's what's on my
board certificate, which was signed by both Virchow and Rokitansky....

"Periodic acid" is pronounced purr-eye-OH-dik. The word is derived
from "iodic" (eye-OH-dik, derived from "iodine"), with the per-
referring to a higher oxidation state, as in "peroxide".

Period.

Bob Richmond
Samurai Pathologist
Knoxville TN

From talulahgosh <@t> gmail.com  Mon Jun 13 15:56:41 2011
From: talulahgosh <@t> gmail.com (Emily Sours)
Date: Mon Jun 13 15:56:45 2011
Subject: [Histonet] Re: Anatomicalalal
In-Reply-To: 
References: 
Message-ID: 

I'm going to start using this pronunciation.  It's so hardcore!

A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Mon, Jun 13, 2011 at 4:54 PM, Robert Richmond wrote:

>
>
> "Periodic acid" is pronounced purr-eye-OH-dik. The word is derived
> from "iodic" (eye-OH-dik, derived from "iodine"), with the per-
> referring to a higher oxidation state, as in "peroxide".
>
> Period.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From victor <@t> pathology.washington.edu  Mon Jun 13 16:07:39 2011
From: victor <@t> pathology.washington.edu (Victor Tobias)
Date: Mon Jun 13 16:08:07 2011
Subject: [Histonet] Pathologic the Game
In-Reply-To: 
References: 
	
Message-ID: <4DF67C1B.20008@pathology.washington.edu>

Here is something for the evening entertainment at the next NSH conference.

http://www.gamespot.com/pc/adventure/pathologic/index.html

Victor Tobias HT(ASCP)
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor@pathology.washington.edu
206-744-2735
206-744-8240 Fax
=================================================
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
of the intended recipients. If you are not the intended recipient, or
if the message has been addressed to you in error, do not read,
disclose, reproduce, distribute, disseminate or otherwise use this
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On 6/13/2011 1:56 PM, Emily Sours wrote:
> I'm going to start using this pronunciation.  It's so hardcore!
>
> A great book should leave you with many experiences, and slightly exhausted.
> You should live several lives while reading it.
> -William Styron
>
>
>
> On Mon, Jun 13, 2011 at 4:54 PM, Robert Richmondwrote:
>
>>
>> "Periodic acid" is pronounced purr-eye-OH-dik. The word is derived
>> from "iodic" (eye-OH-dik, derived from "iodine"), with the per-
>> referring to a higher oxidation state, as in "peroxide".
>>
>> Period.
>>
>> Bob Richmond
>> Samurai Pathologist
>> Knoxville TN
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Karen.Kay <@t> albertahealthservices.ca  Mon Jun 13 16:41:57 2011
From: Karen.Kay <@t> albertahealthservices.ca (Karen Kay)
Date: Mon Jun 13 16:42:21 2011
Subject: [Histonet] RADIOACTIVE SEEDS IN PROSTATE TISSUE
In-Reply-To: <20110613200954.529EE20EFF7@scutcheon.calgaryhealthregion.ca>
References: <20110613200954.529EE20EFF7@scutcheon.calgaryhealthregion.ca>
Message-ID: <14E77BDEC6B22749B75CAC82EC2090FF03266C3D29@EXMBXC5.crha.bewell.ca>

Good Afternoon Everyone,
Does anyone have any information on the safety aspects of handling prostate tissue that has had radioactive seeds implanted once it is submitted for pathological examination. I am asking about all phases of the examination from gross room, embedding, microtomy, staining.I have done a search on the subject via the Archives and have only seen one reference to this issue which was a similar question posed by Peggy Wenk in 2009.
Thanks very much
Karen

Karen J Kay, MLT
Supervisor - Histopathology and Cytology Laboratory
Chinook Regional Hospital
South Zone West - Alberta Health Services
960 - 19 Street South
Lethbridge, Alberta
T1J 1W5
Phone: (403) 388-6061
Fax: (403) 388-6067
karen.kay@albertahealthservices.ca

This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you.

From bakevictoria <@t> gmail.com  Mon Jun 13 16:53:49 2011
From: bakevictoria <@t> gmail.com (Victoria Baker)
Date: Mon Jun 13 16:53:53 2011
Subject: [Histonet] Re: Anatomicalalal
In-Reply-To: 
References: 
	
Message-ID: 

I've been reading all of this and just lovin' it!  Anatomic Pathology or
Clinical & Anatomic Pathology.  It was also once told to me that Anatomic
Pathology was the "ugly step sister" which is why getting $$'s was
difficult.
But I have another question is it Tol-U-i-dine blue or Tol-id-ei-on blue?
"Tom-A-toe ,Tom-a-tow, Pot-A-toe, Pot-a-tow let's call the whole thing
off".  Isn't that how the song goes?  Sorry I couldn't resist.  Have a happy
night.  Sara my $5 is on it's way... now you can get an extra paddle for
your row boat!
Vikki
 On Jun 13, 2011 4:56 PM, "Emily Sours"  wrote:
> I'm going to start using this pronunciation. It's so hardcore!
>
> A great book should leave you with many experiences, and slightly
exhausted.
> You should live several lives while reading it.
> -William Styron
>
>
>
> On Mon, Jun 13, 2011 at 4:54 PM, Robert Richmond wrote:
>
>>
>>
>> "Periodic acid" is pronounced purr-eye-OH-dik. The word is derived
>> from "iodic" (eye-OH-dik, derived from "iodine"), with the per-
>> referring to a higher oxidation state, as in "peroxide".
>>
>> Period.
>>
>> Bob Richmond
>> Samurai Pathologist
>> Knoxville TN
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From rsrichmond <@t> gmail.com  Mon Jun 13 17:02:52 2011
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Mon Jun 13 17:02:56 2011
Subject: [Histonet] Re: Anatomicalalal
In-Reply-To: 
References: 
	
	
Message-ID: 

Vikki Baker notes:

>>I've been reading all of this and just lovin' it!  Anatomic Pathology or Clinical & Anatomic Pathology.  It was also once told to me that Anatomic Pathology was the "ugly step sister" which is why getting $$'s was difficult.
But I have another question is it Tol-U-i-dine blue or Tol-id-ei-on
blue?  "Tom-A-toe ,Tom-a-tow, Pot-A-toe, Pot-a-tow let's call the
whole thing off".  Isn't that how the song goes?  Sorry I couldn't
resist.  Have a happy night.  Sara my $5 is on it's way... now you can
get an extra paddle for your row boat!
Vikki<<

"Toluidine blue" is tuh-LOO-uh-din or tuh-LYOO-uh-din depending on
where you come from in the English language. I've also heard
tuh-LOO-uh-deen.

Anatomic pathology is indeed often referred to as the "ugly
stepsister" or "red-haired stepchild" because of its isolated lowly
position in the clinical laboratory, a situation that's gotten
somewhat worse in my near half a century in pathology.

Bob Richmond
Samurai Pathologist
Knoxville TN

From bakevictoria <@t> gmail.com  Mon Jun 13 17:11:49 2011
From: bakevictoria <@t> gmail.com (Victoria Baker)
Date: Mon Jun 13 17:11:53 2011
Subject: [Histonet] Re: Anatomicalalal
In-Reply-To: 
References: 
	
	
	
Message-ID: 

Thanks Bob.  English isn't my first language...so I sound things out a
little differently.
I've heard several different pronunciations in several different parts of
the east coast and mid- west.   I just had to have some fun.  Vikki
On Jun 13, 2011 6:02 PM, "Robert Richmond"  wrote:
> Vikki Baker notes:
>
>>>I've been reading all of this and just lovin' it! Anatomic Pathology or
Clinical & Anatomic Pathology. It was also once told to me that Anatomic
Pathology was the "ugly step sister" which is why getting $$'s was
difficult.
> But I have another question is it Tol-U-i-dine blue or Tol-id-ei-on
> blue? "Tom-A-toe ,Tom-a-tow, Pot-A-toe, Pot-a-tow let's call the
> whole thing off". Isn't that how the song goes? Sorry I couldn't
> resist. Have a happy night. Sara my $5 is on it's way... now you can
> get an extra paddle for your row boat!
> Vikki<<
>
> "Toluidine blue" is tuh-LOO-uh-din or tuh-LYOO-uh-din depending on
> where you come from in the English language. I've also heard
> tuh-LOO-uh-deen.
>
> Anatomic pathology is indeed often referred to as the "ugly
> stepsister" or "red-haired stepchild" because of its isolated lowly
> position in the clinical laboratory, a situation that's gotten
> somewhat worse in my near half a century in pathology.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
From LSebree <@t> uwhealth.org  Mon Jun 13 17:18:10 2011
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Mon Jun 13 17:18:15 2011
Subject: [Histonet] OT: How do you call...
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: 

Anatomic Pathology here. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Monday, June 13, 2011 1:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] OT: How do you call...

I've figured out how to fund  my approaching retirement!  A poll!  So...
is the correct term "Anatomical Pathology" or "Anatomic Pathology"?
Send $5.00 with your email  reply and I'll have the down payment for
that tropical island with internet.  Seriously - what is the consensus
and/or the correct usage?  Figure I better get all these Deep Questions
answered while I have the chance.  (P.S., I'm only kidding about the
$5.00 but I do take PayPal).

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From AnthonyH <@t> chw.edu.au  Mon Jun 13 18:35:20 2011
From: AnthonyH <@t> chw.edu.au (Tony Henwood)
Date: Mon Jun 13 18:35:35 2011
Subject: [Histonet] RE: Decal Solutions
In-Reply-To: 
References: 
Message-ID: <6D6BD1DE8A5571489398B392A38A715718875D8D@xmdb02.nch.kids>

Vicki,

We fix our trephines in 10% formal alcohol overnight and decal for 6 hours in formic acid (4% Formaldehyde, 33% Formic acid, 0.85% Sodium Chloride).

Excellent morphology and good antigen preservation.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki
Sent: Tuesday, 14 June 2011 12:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Decal Solutions

Hi,
Does anyone know of any decal solutions that are compatible with IHC, ISH, FISH other than EDTA and solutions containing HCL ?  We have heard that some people use formic acid.  Also, what would be the decalcification times for tissue such as a bone marrow bx using these other solutions ?  Any help would be greatly appreciated...

Thanks,
Vicki Gauch
AMCH
Albany, NY



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From khairedai <@t> yahoo.com  Tue Jun 14 04:09:54 2011
From: khairedai <@t> yahoo.com (Khaire Dai)
Date: Tue Jun 14 04:11:41 2011
Subject: [Histonet] Dry ice alternatives?
Message-ID: <561862.55262.qm@web113006.mail.gq1.yahoo.com>

Hi

I was supposed to transfer the cryosections from -80 freezer to the other place for staining.
I dont have dry ice for the transport.

It is in the same building but will take about 25 mins. before i start fixing/process the slides.

How can i transport them if any common method and/or creative ideas.

Thank you in advance for the support.

Khaire


From ree3 <@t> leicester.ac.uk  Tue Jun 14 04:22:12 2011
From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.)
Date: Tue Jun 14 04:22:20 2011
Subject: [Histonet] RE: Anatomicalalal
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF747@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF747@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <7722595275A4DD4FA225B92CDBF174A10176874C7091@EXC-MBX3.cfs.le.ac.uk>

How about just plain old " Histopathology"??

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: 13 June 2011 20:03
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Anatomicalalal

I just LOVE it when I'm right - and it happens so rarely!  The consensus
of ten responders so far is ANATOMIC.  This just makes sense to me - we
don't call them Diagnostical Pathologists, do we?  Nah.  Keep those
correct answers coming my way - I needed the confirmation!  And I have
$50 toward the rowboat that'll take me to that island.  Can you tell I
need the diversion today?

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From patpxs <@t> gmail.com  Tue Jun 14 05:56:31 2011
From: patpxs <@t> gmail.com (Paula Sicurello)
Date: Tue Jun 14 05:56:35 2011
Subject: [Histonet] OT: How do you call...
In-Reply-To: 
References: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>
	
Message-ID: 

This reminds me of a joke I learned as a kid.  How to use analyze and
anatomy in a sentence (song actually).

"My analyze over the ocean,
my analyze over the sea,
my analyze over the ocean,
so bring back my anatomy."

:-)

-- 
Paula Sicurello, HTL (ASCP)
Supervisor, Electron Microscope Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P:  919.684.2091



On Mon, Jun 13, 2011 at 6:18 PM, Sebree Linda A  wrote:
> Anatomic Pathology here.
>
>
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> DB1-223 VAH
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden,
> Sara
> Sent: Monday, June 13, 2011 1:34 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] OT: How do you call...
>
> I've figured out how to fund ?my approaching retirement! ?A poll! ?So...
> is the correct term "Anatomical Pathology" or "Anatomic Pathology"?
> Send $5.00 with your email ?reply and I'll have the down payment for
> that tropical island with internet. ?Seriously - what is the consensus
> and/or the correct usage? ?Figure I better get all these Deep Questions
> answered while I have the chance. ?(P.S., I'm only kidding about the
> $5.00 but I do take PayPal).
>
>
>
> Sally Breeden, HT(ASCP)
>
> New Mexico Department of Agriculture
>
> Veterinary Diagnostic Services
>
> 1101 Camino de Salud NE
>
> Albuquerque, NM ?87102
>
> 505-383-9278?(Histology Lab)
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

From micropathlabs <@t> yahoo.com  Tue Jun 14 08:30:12 2011
From: micropathlabs <@t> yahoo.com (Sheila Haas)
Date: Tue Jun 14 08:30:15 2011
Subject: [Histonet] Temperature monitoring system
Message-ID: <924545.75382.qm@web161712.mail.bf1.yahoo.com>

We are looking for a system to monitor a couple of our
lab refrigerators containing expensive antibodies and probes.
Can anyone recommend a small, basic system?
Thank you in advance.
Sheila
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
From TMcNemar <@t> lmhealth.org  Tue Jun 14 10:14:06 2011
From: TMcNemar <@t> lmhealth.org (Tom McNemar)
Date: Tue Jun 14 10:14:13 2011
Subject: [Histonet] Temperature monitoring system
In-Reply-To: <924545.75382.qm@web161712.mail.bf1.yahoo.com>
References: <924545.75382.qm@web161712.mail.bf1.yahoo.com>
Message-ID: 

All of our laboratory refrigerators and freezers are monitored by a system from Rees Scientific.  In Histology we only have one refrigerator that stores our antibodies and we check and record the temperature daily.  We do have our tissue processor connected to this alarm system since there is no in the vicinity after 4pm. It may be more that you want or need but they can probably tailor a package to your needs.  You can find them online.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar@lmhealth.org
www.LMHealth.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Tuesday, June 14, 2011 9:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Temperature monitoring system

We are looking for a system to monitor a couple of our
lab refrigerators containing expensive antibodies and probes.
Can anyone recommend a small, basic system?
Thank you in advance.
Sheila

Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.

From micropathlabs <@t> yahoo.com  Tue Jun 14 10:17:22 2011
From: micropathlabs <@t> yahoo.com (Sheila Haas)
Date: Tue Jun 14 10:17:25 2011
Subject: [Histonet] Thank you
Message-ID: <787132.75201.qm@web161703.mail.bf1.yahoo.com>

Thank you to everyone for their assistance with the temperature monitoring. I've 
already made a couple of calls and am finding exactly what we need.
Thanks again.
Sheila
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
From leiker <@t> buffalo.edu  Tue Jun 14 10:31:09 2011
From: leiker <@t> buffalo.edu (Merced Leiker)
Date: Tue Jun 14 10:31:13 2011
Subject: [Histonet] OT: How do you call...
In-Reply-To: 
References: <4D14F0FC9316DD41972D5F03C070908B051DF745@nmdamailsvr.nmda.ad.nmsu.edu>	
	
Message-ID: <4DF77EBD.5080101@buffalo.edu>

hahaha that's pretty clever, actually!

On 6/14/2011 6:56 AM, Paula Sicurello wrote:
> This reminds me of a joke I learned as a kid.  How to use analyze and
> anatomy in a sentence (song actually).
>
> "My analyze over the ocean,
> my analyze over the sea,
> my analyze over the ocean,
> so bring back my anatomy."
>
> :-)
>


From Ronald.Houston <@t> nationwidechildrens.org  Tue Jun 14 10:38:25 2011
From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald)
Date: Tue Jun 14 10:38:31 2011
Subject: [Histonet] Ki-67/PHH3 double staining
Message-ID: 

Has anyone had success double staining these two antibodies for IHC? Ki-67 tends to mask most of the PHH3 staining irrespective of which antibody we use first. Individually, both antibodies have clean and crisp localization. I have seen pictures in articles and on-line of results but am far from attaining anything like these.
We are using the Refine DAB and AP Red kits from Leica on the Bonds. Ki-67 is from Leica and PHH3 from Cell Marque

Any help or suggestions welcome
Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster



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From PMonfils <@t> Lifespan.org  Tue Jun 14 11:00:56 2011
From: PMonfils <@t> Lifespan.org (Monfils, Paul)
Date: Tue Jun 14 11:01:04 2011
Subject: [Histonet] Dry ice alternatives?
In-Reply-To: <561862.55262.qm@web113006.mail.gq1.yahoo.com>
References: <561862.55262.qm@web113006.mail.gq1.yahoo.com>
Message-ID: <4EBFF65383B74D49995298C4976D1D5E084A11E9@LSRIEXCH1.lsmaster.lifespan.org>

Place a container of ordinary ice in the -80 for a couple of hours - or
use one of the commercially available "cold packs" like you use for a
picnic lunch, cooled in the -80.



From gu.lang <@t> gmx.at  Tue Jun 14 11:01:49 2011
From: gu.lang <@t> gmx.at (Gudrun Lang)
Date: Tue Jun 14 11:08:35 2011
Subject: AW: [Histonet]  Decal Soloutions
In-Reply-To: <230873.97732.qm@web80407.mail.mud.yahoo.com>
References: <230873.97732.qm@web80407.mail.mud.yahoo.com>
Message-ID: <3AC5430D0D8347B6967BA756C3D050DE@dielangs.at>


We fix overnight in NBF and decal for 8 hours in "Calrite" (10% Formicacid,
5% Formaldehyd).
For FISH we had issues to demonstrate DNA-loci. CISH for kappa- and
lambda-mRNA works.

We tried a variant with 2 hours acid decal and 6 hours EDTA+Ultrasound.
Morphology in HE is ok, but IHC suffers in comparison to acid alone.
But with this short acid decal FISH has worked.
Decision if acid or combined decal is still not done. Few cases that require
FISH versus everyday IHC.

Bye Gudrun




From Ashley.Troutman <@t> Vanderbilt.Edu  Tue Jun 14 11:42:05 2011
From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A)
Date: Tue Jun 14 11:42:10 2011
Subject: [Histonet] Re: Anatomicalalal
Message-ID: <7B310892042DA74CB3590053F424CFE6143EE300EA@ITS-HCWNEM06.ds.Vanderbilt.edu>

We are the anatomicalization of pathology-as opposed to the clinicalization.  We procure the varying degrees of anatomicalatudinizations of the body.  Then we cut 'em, stain 'em and look at 'em under the scope.  Or so I was told.

5 bucks is on the way, Sarah...

Ashley Troutman BS, HT(ASCP) QIHC
Immunohistochemistry Supervisor
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4531
Nashville, TN  37232

Message: 6
Date: Mon, 13 Jun 2011 16:54:41 -0400
From: Robert Richmond 
Subject: [Histonet] Re: Anatomicalalal
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset=ISO-8859-1

Well, I've been a pathologist for embarrassingly close to fifty years, and I've never heard anything but "anatomic", and that's what's on my board certificate, which was signed by both Virchow and Rokitansky....

"Periodic acid" is pronounced purr-eye-OH-dik. The word is derived from "iodic" (eye-OH-dik, derived from "iodine"), with the per- referring to a higher oxidation state, as in "peroxide".

Period.

Bob Richmond
Samurai Pathologist
Knoxville TN

From AGleiberman <@t> cbiolabs.com  Tue Jun 14 11:55:27 2011
From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman)
Date: Tue Jun 14 11:55:31 2011
Subject: [Histonet] RE: Ki-67/PHH3 double staining
In-Reply-To: 
References: 
Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C19B605@cbiolabs05.CBiolabs.local>

Ronnie,
I don't have any problems with 4-color fluorescence staining using mouse monoclonal Ki67 conjugated with AlexaFluor488 from BD Pharmingen and rabbit monoclonal PHH3 conjugated with AlexaFluor 555 from Cell Signaling (I also added for complete set rabbit monoclonal gammaH2A.X conjugated with AF647 from Cell Signaling and DAPI). All four colors work good. There was no any masking for PHH3. It looks like chromosome staining for Ki67 was a little less crisp than in case when I used combination with antibody against non-phosphorilated H2A.X histone instead of Ki67. So, probably there is a little masking of Ki67 rather than PHH3.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman@cbiolabs.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald
Sent: Tuesday, June 14, 2011 11:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ki-67/PHH3 double staining

Has anyone had success double staining these two antibodies for IHC? Ki-67 tends to mask most of the PHH3 staining irrespective of which antibody we use first. Individually, both antibodies have clean and crisp localization. I have seen pictures in articles and on-line of results but am far from attaining anything like these.
We are using the Refine DAB and AP Red kits from Leica on the Bonds. Ki-67 is from Leica and PHH3 from Cell Marque

Any help or suggestions welcome
Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster



----------------------------------------- Confidentiality Notice:
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sole use of the intended recipient(s) and may contain confidential
and privileged information. The recipient is responsible to
maintain the confidentiality of this information and to use the
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This communication may contain privileged information.  It is intended solely for the use of the addressee.  If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information.  If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy.  This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act.  You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information.

From careerstudio <@t> bellsouth.net  Tue Jun 14 12:14:59 2011
From: careerstudio <@t> bellsouth.net (Barbara L. Siegel)
Date: Tue Jun 14 12:15:02 2011
Subject: [Histonet] Histology Line Supervisor for 10:30pm-7:00am shift,
	based in Dallas
Message-ID: <04d701cc2ab6$97d05890$c77109b0$@net>

Our client is a well-respected clinical laboratory currently seeking an
Histology Line Supervisor for their 10:30pm-7:00am shift, based in Dallas,
to be responsible for day-to-day operation of histology laboratory and
supervision of the technical/support staff.    Will provide technical
instructions and training in techniques, instrumentation, and work flow, and
maintain appropriate quality assurance documentation.  

 

Supervisory functions will include hiring, training, competency assessments
and performance appraisals.  Additionally, will facilitate and develop
continuing education programs, and provides backup for bench technicians in
sectioning and staining as needed.  

 

Required for this position is 5+ years of recent experience as a
histotechnician / histotechnologist, with 3-5 years demonstrated success in
a supervisory /  leadership capacity.   BS degree or equivalent education
and experience, HT(ASCP) or HTL (ASCP).   IHC experience helpful.

 

 

Attractive salary is offered, position is bonus eligible and receives a
shift differential.  Please contact June Benney at
 careerstudio@bellsouth.net for more
information.  

 

Career Studio

national search

Palm Beach, FL

 

 

 

 

From pmuhlhau <@t> seattlecca.org  Tue Jun 14 13:17:15 2011
From: pmuhlhau <@t> seattlecca.org (Muhlhauser, Petrina R)
Date: Tue Jun 14 13:18:14 2011
Subject: [Histonet] RE: Pathologic the Game
In-Reply-To: <20110614160951.3264330DB41@mx02.seattlecca.org>
References: <20110614160951.3264330DB41@mx02.seattlecca.org>
Message-ID: <19813_1308075475_4DF7A5D3_19813_21_1_671E1ABBDE2AFC41861509C091AEED2605239D36@EVS01.seattlecca.org>

I can't believe I've never played (or even heard of) this game!

...Oh wait, yes I can. Average 6/10 review? Ouch! Might have to look it
up anyway just because of the name, though. >_>

Petrina Muhlhauser
Clinical Laboratory Technician
Seattle Cancer Care Alliance
Department of Pathology

-----Original Message-----
Message: 8
Date: Mon, 13 Jun 2011 14:07:39 -0700
From: Victor Tobias 
Subject: [Histonet] Pathologic the Game
To: histonet@lists.utsouthwestern.edu
Message-ID: <4DF67C1B.20008@pathology.washington.edu>
Content-Type: text/plain; charset=UTF-8; format=flowed

Here is something for the evening entertainment at the next NSH
conference.

http://www.gamespot.com/pc/adventure/pathologic/index.html

Victor Tobias HT(ASCP)
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor@pathology.washington.edu
206-744-2735
206-744-8240 Fax
=================================================
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From rsrichmond <@t> gmail.com  Tue Jun 14 13:24:05 2011
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Tue Jun 14 13:24:10 2011
Subject: [Histonet] Re: Histonet Digest, Vol 91, Issue 19
In-Reply-To: <4df787d7.0b6f970a.65f1.ffffe0b1SMTPIN_ADDED@mx.google.com>
References: <4df787d7.0b6f970a.65f1.ffffe0b1SMTPIN_ADDED@mx.google.com>
Message-ID: 

Karen J Kay, MLT, Supervisor - Histopathology and Cytology Laboratory,
Chinook Regional Hospital, Lethbridge, Alberta, Canada asks:

>>Does anyone have any information on the safety aspects of handling prostate tissue that has had radioactive seeds implanted once it is submitted for pathological examination? I am asking about all phases of the examination from gross room, embedding, microtomy, staining. I have done a search on the subject via the Archives and have only seen one reference to this issue which was a similar question posed by Peggy Wenk in 2009.<<

I posted about this a couple of years ago - found my post in
Histosearch using the search term "iridium".

http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-October/039840.html

This post will give you the half-lives of the three radioisotopes
commonly used. It's conventional to assume that radioactive material
is hazardous until ten half-lives have gone by - about two years for
the most commonly used isotopes.

This is a question to pose to your hospital's radiation safety
officer, if you can find him. Back in the days when procedure manuals
functioned, I'd have wanted a written procedure for dealing with this
situation. Yes, the "seeds" are potentially hazardous.

I've posted a photograph I took of some of these "seeds" a few years ago at
http://www.flickr.com/photos/bobrichmond/5833004125/in/photostream

Bob Richmond
Samurai Pathologist
Knoxville TN

From rsrichmond <@t> gmail.com  Tue Jun 14 13:27:32 2011
From: rsrichmond <@t> gmail.com (Robert Richmond)
Date: Tue Jun 14 13:27:37 2011
Subject: [Histonet] Radioactive "seeds" in prostate tissue
Message-ID: 

Sorry - I sent an incomplete post a few minutes ago.
******************************
Karen J Kay, MLT, Supervisor - Histopathology and Cytology Laboratory,
Chinook Regional Hospital, Lethbridge, Alberta, Canada asks:

>>Does anyone have any information on the safety aspects of handling prostate tissue that has had radioactive seeds implanted once it is submitted for pathological examination? I am asking about all phases of the examination from gross room, embedding, microtomy, staining. I have done a search on the subject via the Archives and have only seen one reference to this issue which was a similar question posed by Peggy Wenk in 2009.<<

I posted about this a couple of years ago - found my post in
Histosearch using the search term "iridium".

http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-October/039840.html

This post will give you the half-lives of the three radioisotopes
commonly used. It's conventional to assume that radioactive material
is hazardous until ten half-lives have gone by - about two years for
the most commonly used isotopes.

This is a question to pose to your hospital's radiation safety
officer, if you can find him. Back in the days when procedure manuals
functioned, I'd have wanted a written procedure for dealing with this
situation. Yes, the "seeds" are potentially hazardous.

I've posted a photograph I took of some of these "seeds" a few years ago at
http://www.flickr.com/photos/bobrichmond/5833004125/in/photostream

Bob Richmond
Samurai Pathologist
Knoxville TN

From sbreeden <@t> nmda.nmsu.edu  Tue Jun 14 13:35:25 2011
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Tue Jun 14 13:35:30 2011
Subject: [Histonet] ANATOMIC Pathology
Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF754@nmdamailsvr.nmda.ad.nmsu.edu>

With one exception (who shall remain nameless unless you want to send me
another $5.00...), the Consensus of Professional Opinion Including the
Samurai Pathologist (The Knower of All Things Histologic[al]) - I
christen thee ANATOMIC Pathology.  And thank you, all, for allowing me
my leap across to the Land of Nonsensic[al]ness).   I feel so much
better for having caused a ripple in the cosmic continuum.  Without
further ado, I shall count my money and dream of that pathologist-less
island (sorry, guys).

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

From christiegowan <@t> msn.com  Tue Jun 14 13:56:10 2011
From: christiegowan <@t> msn.com (CHRISTIE GOWAN)
Date: Tue Jun 14 13:56:16 2011
Subject: [Histonet] ANATOMIC Pathology
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF754@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF754@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: 


Sara,
I have truly enjoyed this thread. Thanks for getting it started. Best of luck to you in the future.
 
 
Christie Gowan HT(ASCP)
Histology Supervisor
Surgical Pathology Laboratories
UAB Hospital
(205) 934-4991
cgowan@uabmc.edu

 

> Date: Tue, 14 Jun 2011 12:35:25 -0600
> From: sbreeden@nmda.nmsu.edu
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] ANATOMIC Pathology
> 
> With one exception (who shall remain nameless unless you want to send me
> another $5.00...), the Consensus of Professional Opinion Including the
> Samurai Pathologist (The Knower of All Things Histologic[al]) - I
> christen thee ANATOMIC Pathology. And thank you, all, for allowing me
> my leap across to the Land of Nonsensic[al]ness). I feel so much
> better for having caused a ripple in the cosmic continuum. Without
> further ado, I shall count my money and dream of that pathologist-less
> island (sorry, guys).
> 
> 
> 
> Sally Breeden, HT(ASCP)
> 
> New Mexico Department of Agriculture
> 
> Veterinary Diagnostic Services
> 
> 1101 Camino de Salud NE
> 
> Albuquerque, NM 87102
> 
> 505-383-9278 (Histology Lab)
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From lblazek <@t> digestivespecialists.com  Tue Jun 14 13:58:54 2011
From: lblazek <@t> digestivespecialists.com (Blazek, Linda)
Date: Tue Jun 14 13:59:05 2011
Subject: [Histonet] RE: ANATOMIC Pathology
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF754@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF754@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBF65E9F3@IBMB7Exchange.digestivespecialists.com>

And we all can tell you are creeping nearer and nearer that non pathologic (al) island oasis in the toluidine blue sea of retirement. Periodically I think of that time but it's not near enough.

Linda

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Tuesday, June 14, 2011 2:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ANATOMIC Pathology

With one exception (who shall remain nameless unless you want to send me
another $5.00...), the Consensus of Professional Opinion Including the
Samurai Pathologist (The Knower of All Things Histologic[al]) - I
christen thee ANATOMIC Pathology.  And thank you, all, for allowing me
my leap across to the Land of Nonsensic[al]ness).   I feel so much
better for having caused a ripple in the cosmic continuum.  Without
further ado, I shall count my money and dream of that pathologist-less
island (sorry, guys).

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From NMargaryan <@t> childrensmemorial.org  Tue Jun 14 13:57:28 2011
From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira)
Date: Tue Jun 14 14:00:25 2011
Subject: [Histonet] How do you call...
Message-ID: 

I totally agree with Rene J.: there is anatomy and physiology becomes pathological that is why need to call "Pathological Anatomy" and "Pathological Physiology"

Foreign people learn what they hear usually that is why Americans have to watch there language when speaking and use only correct words!

Naira

From sbreeden <@t> nmda.nmsu.edu  Tue Jun 14 14:01:24 2011
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Tue Jun 14 14:01:28 2011
Subject: [Histonet] Retirement
Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF759@nmdamailsvr.nmda.ad.nmsu.edu>

Some of my compatriots are laboring under the assumption that retirement
is sooner than later.  If it were only true... my planned Date of
Departure (pending an Offer I Cannot Refuse) is 29 February 2012.  I
have many ideas for interrupting your days until then...  Fear not!

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

From JWeems <@t> sjha.org  Tue Jun 14 14:13:30 2011
From: JWeems <@t> sjha.org (Weems, Joyce)
Date: Tue Jun 14 14:13:36 2011
Subject: [Histonet] RE: Retirement
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF759@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF759@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408210EA5F3@CHEXCMS10.one.ads.che.org>

All the atom i comicals out there.. Keep it coming!! 


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Tuesday, June 14, 2011 15:01
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retirement

Some of my compatriots are laboring under the assumption that retirement is sooner than later.  If it were only true... my planned Date of Departure (pending an Offer I Cannot Refuse) is 29 February 2012.  I have many ideas for interrupting your days until then...  Fear not!

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This e-mail, including any attachments is the 
property of Catholic Health East and is intended 
for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are 
not the intended recipient, please delete this message, and 
reply to the sender regarding the error in a separate email. 
 


From lblazek <@t> digestivespecialists.com  Tue Jun 14 14:25:50 2011
From: lblazek <@t> digestivespecialists.com (Blazek, Linda)
Date: Tue Jun 14 14:25:56 2011
Subject: [Histonet] RE: Retirement
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF759@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF759@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBF65E9F7@IBMB7Exchange.digestivespecialists.com>

7 years compared to 8 months is gigantically significant. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Tuesday, June 14, 2011 3:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retirement

Some of my compatriots are laboring under the assumption that retirement
is sooner than later.  If it were only true... my planned Date of
Departure (pending an Offer I Cannot Refuse) is 29 February 2012.  I
have many ideas for interrupting your days until then...  Fear not!

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From sgoebel <@t> mirnarx.com  Tue Jun 14 14:30:41 2011
From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com)
Date: Tue Jun 14 14:30:44 2011
Subject: [Histonet] RE: Retirement
In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EBF65E9F7@IBMB7Exchange.digestivespecialists.com>
References: <4D14F0FC9316DD41972D5F03C070908B051DF759@nmdamailsvr.nmda.ad.nmsu.edu>
	<5A2BD13465E061429D6455C8D6B40E390EBF65E9F7@IBMB7Exchange.digestivespecialists.com>
Message-ID: 

I'm 30, so blah blah blah...I have 30 or 40 more YEARS!!!!!!
=)

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Tuesday, June 14, 2011 2:26 PM
To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Retirement

7 years compared to 8 months is gigantically significant. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Tuesday, June 14, 2011 3:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retirement

Some of my compatriots are laboring under the assumption that retirement
is sooner than later.  If it were only true... my planned Date of
Departure (pending an Offer I Cannot Refuse) is 29 February 2012.  I
have many ideas for interrupting your days until then...  Fear not!

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Rcartun <@t> harthosp.org  Tue Jun 14 15:36:27 2011
From: Rcartun <@t> harthosp.org (Richard Cartun)
Date: Tue Jun 14 15:36:36 2011
Subject: [Histonet] HCV testing
Message-ID: <4DF78E0B.7400.0077.1@harthosp.org>

Is anyone doing PCR testing for Hepatitis C Virus on formalin-fixed, paraffin-embedded tissues?  If so, can you find the virus in tissues other than liver?  Maybe in blood within vessels?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



From rosenfeldtek <@t> hotmail.com  Tue Jun 14 17:44:16 2011
From: rosenfeldtek <@t> hotmail.com (JR R)
Date: Tue Jun 14 17:44:19 2011
Subject: [Histonet] Keeping Histo room floor clean?
Message-ID: 


We keep getting a lot of paraffin on the floor of one histo room--especially around the microtome and the embedding station.

Short of laying down a tarp, what do folks do keep wax off of the floor?

Thanks,

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology
 		 	   		  
From histotech <@t> imagesbyhopper.com  Tue Jun 14 19:49:57 2011
From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com)
Date: Tue Jun 14 19:50:17 2011
Subject: [Histonet] Keeping Histo room floor clean?
In-Reply-To: 
References: 
Message-ID: <54B2B63A-3054-414A-9D4C-97241B2505E7@imagesbyhopper.com>

We don't keep it off the floor, but do use a wide-bladed putty knife attached to a mop handle to scrape the residual wax off the floor. It woks quite nicely and doesn't remove the actual floor wax like a razor blade scrapper would.

Michelle

Sent from my iPhone

On Jun 14, 2011, at 6:44 PM, JR R  wrote:

> 
> We keep getting a lot of paraffin on the floor of one histo room--especially around the microtome and the embedding station.
> 
> Short of laying down a tarp, what do folks do keep wax off of the floor?
> 
> Thanks,
> 
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
>                         _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From histotech <@t> imagesbyhopper.com  Tue Jun 14 19:52:46 2011
From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com)
Date: Tue Jun 14 19:52:55 2011
Subject: [Histonet] RE: Retirement
In-Reply-To: 
References: <4D14F0FC9316DD41972D5F03C070908B051DF759@nmdamailsvr.nmda.ad.nmsu.edu>
	<5A2BD13465E061429D6455C8D6B40E390EBF65E9F7@IBMB7Exchange.digestivespecialists.com>
	
Message-ID: <9C28315E-AB38-4F5E-BC28-B2C057278470@imagesbyhopper.com>

I have kids going to, or getting ready to go to, college.  I sure don't see retirement in my near furture!!

Michelle


On Jun 14, 2011, at 3:30 PM,  wrote:

> I'm 30, so blah blah blah...I have 30 or 40 more YEARS!!!!!!
> =)
> 
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas  78744
> (512)901-0900 ext. 6912
> 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek,
> Linda
> Sent: Tuesday, June 14, 2011 2:26 PM
> To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: Retirement
> 
> 7 years compared to 8 months is gigantically significant. 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden,
> Sara
> Sent: Tuesday, June 14, 2011 3:01 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Retirement
> 
> Some of my compatriots are laboring under the assumption that retirement
> is sooner than later.  If it were only true... my planned Date of
> Departure (pending an Offer I Cannot Refuse) is 29 February 2012.  I
> have many ideas for interrupting your days until then...  Fear not!
> 
> 
> 
> Sally Breeden, HT(ASCP)
> 
> New Mexico Department of Agriculture
> 
> Veterinary Diagnostic Services
> 
> 1101 Camino de Salud NE
> 
> Albuquerque, NM  87102
> 
> 505-383-9278 (Histology Lab)
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From histotech <@t> imagesbyhopper.com  Tue Jun 14 19:55:26 2011
From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com)
Date: Tue Jun 14 19:55:41 2011
Subject: [Histonet] RE: ANATOMIC Pathology
In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EBF65E9F3@IBMB7Exchange.digestivespecialists.com>
References: <4D14F0FC9316DD41972D5F03C070908B051DF754@nmdamailsvr.nmda.ad.nmsu.edu>
	<5A2BD13465E061429D6455C8D6B40E390EBF65E9F3@IBMB7Exchange.digestivespecialists.com>
Message-ID: <27D64C53-52B6-49A8-AB5B-52229F8BCF1A@imagesbyhopper.com>

:-D @ Linda!

Fun thread Sara!  :o)

Michelle



On Jun 14, 2011, at 2:58 PM, "Blazek, Linda"  wrote:

> And we all can tell you are creeping nearer and nearer that non pathologic (al) island oasis in the toluidine blue sea of retirement. Periodically I think of that time but it's not near enough.
> 
> Linda
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
> Sent: Tuesday, June 14, 2011 2:35 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] ANATOMIC Pathology
> 
> With one exception (who shall remain nameless unless you want to send me
> another $5.00...), the Consensus of Professional Opinion Including the
> Samurai Pathologist (The Knower of All Things Histologic[al]) - I
> christen thee ANATOMIC Pathology.  And thank you, all, for allowing me
> my leap across to the Land of Nonsensic[al]ness).   I feel so much
> better for having caused a ripple in the cosmic continuum.  Without
> further ado, I shall count my money and dream of that pathologist-less
> island (sorry, guys).
> 
> 
> 
> Sally Breeden, HT(ASCP)
> 
> New Mexico Department of Agriculture
> 
> Veterinary Diagnostic Services
> 
> 1101 Camino de Salud NE
> 
> Albuquerque, NM  87102
> 
> 505-383-9278 (Histology Lab)
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From AnthonyH <@t> chw.edu.au  Tue Jun 14 20:14:49 2011
From: AnthonyH <@t> chw.edu.au (Tony Henwood)
Date: Tue Jun 14 20:15:11 2011
Subject: [Histonet] RE: Retirement
In-Reply-To: <9C28315E-AB38-4F5E-BC28-B2C057278470@imagesbyhopper.com>
References: <4D14F0FC9316DD41972D5F03C070908B051DF759@nmdamailsvr.nmda.ad.nmsu.edu>
	<5A2BD13465E061429D6455C8D6B40E390EBF65E9F7@IBMB7Exchange.digestivespecialists.com>
	 
	<9C28315E-AB38-4F5E-BC28-B2C057278470@imagesbyhopper.com>
Message-ID: <6D6BD1DE8A5571489398B392A38A71571887737A@xmdb02.nch.kids>

I overheard my wife and daughter discussing clothes, toys etc for perspective grandchildren and have come to realise that I can't afford to retire, I will need to continue to work to keep my future grandchildren in the lifestyle  that will be accustomed to!

Not that I am a Grandfather yet (though I would probably be the last to know).

But more importantly I am too young to be married to a Grandmother.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com
Sent: Wednesday, 15 June 2011 10:53 AM
To: 
Cc: 
Subject: Re: [Histonet] RE: Retirement

I have kids going to, or getting ready to go to, college.  I sure don't see retirement in my near furture!!

Michelle


On Jun 14, 2011, at 3:30 PM,  wrote:

> I'm 30, so blah blah blah...I have 30 or 40 more YEARS!!!!!!
> =)
> 
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas  78744
> (512)901-0900 ext. 6912
> 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of 
> Blazek, Linda
> Sent: Tuesday, June 14, 2011 2:26 PM
> To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: Retirement
> 
> 7 years compared to 8 months is gigantically significant. 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of 
> Breeden, Sara
> Sent: Tuesday, June 14, 2011 3:01 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Retirement
> 
> Some of my compatriots are laboring under the assumption that 
> retirement is sooner than later.  If it were only true... my planned 
> Date of Departure (pending an Offer I Cannot Refuse) is 29 February 
> 2012.  I have many ideas for interrupting your days until then...  Fear not!
> 
> 
> 
> Sally Breeden, HT(ASCP)
> 
> New Mexico Department of Agriculture
> 
> Veterinary Diagnostic Services
> 
> 1101 Camino de Salud NE
> 
> Albuquerque, NM  87102
> 
> 505-383-9278 (Histology Lab)
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
*********************************************************************************

From Clough <@t> medicine.tamhsc.edu  Tue Jun 14 21:52:29 2011
From: Clough <@t> medicine.tamhsc.edu (Clough, Bret)
Date: Tue Jun 14 21:53:12 2011
Subject: [Histonet] Paraffin processing and embeding of Chondrocyte pellets.
Message-ID: 

Hello Everyone,
 I was wondering if anyone has a protocol for paraffin embedding chondrocyte pellets that they would be willing to share with me? Any help would be greatly appreciated.

Thanks,
     Bret

Bret Clough
TAMHSC

From tora.bardal <@t> bio.ntnu.no  Wed Jun 15 04:16:10 2011
From: tora.bardal <@t> bio.ntnu.no (Tora Bardal)
Date: Wed Jun 15 04:16:17 2011
Subject: [Histonet] decal fish larvae
Message-ID: <4DF8785A.3000704@bio.ntnu.no>

Hi

Does anyone have a suggestion for decal solution/ time for (weakly) 
ossified bone in fish larvae?  Paraffin section tends to split due to 
the harder tissue, nice longitudinal section are hard to get.

------------------------------------------------------------------------

Tora Bardal
Overingeni?r/ Senior Engineer
NTNU Senter for fiskeri og havbruk /NTNU Center of Fisheries and Aquaculture
NTNU, 7491 Trondheim


From GauchV <@t> mail.amc.edu  Wed Jun 15 06:05:48 2011
From: GauchV <@t> mail.amc.edu (Gauch, Vicki)
Date: Wed Jun 15 06:05:38 2011
Subject: [Histonet] Thank You
Message-ID: 

Thank you to everyone who responded to my decal solution for IHC question....your help was greatly appreciated...
Have a great day,
Vicki Gauch
AMCH
Albany, NY



-----------------------------------------
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From d.a.faichney <@t> stir.ac.uk  Wed Jun 15 07:44:28 2011
From: d.a.faichney <@t> stir.ac.uk (Debbie Faichney)
Date: Wed Jun 15 07:45:05 2011
Subject: [Histonet] decal fish larvae
In-Reply-To: <4DF8785A.3000704@bio.ntnu.no>
References: <4DF8785A.3000704@bio.ntnu.no>
Message-ID: <8ED3F2CA5B78E142B8193376C57330F8F854375438@EXCH2007.ad.stir.ac.uk>

Hello Tora,

We surface decal the trimmed block, for one hour, using a commercial product called Rapid Decalcifier (Cellpath, UK). I don't know what it contains, other than Hydrochloric acid, but it works a treat.  Other alternatives for fish have been Nair hair removal cream (applied to the surface for 10 mins or so) and fabric softner (dilute 1:10 in distilled water and soak for 30 min to an hour)
Hope you are successful

Debbie Faichney
Institute of Aquaculture
University of Stirling
Scotland

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tora Bardal
Sent: 15 June 2011 10:16
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decal fish larvae

Hi

Does anyone have a suggestion for decal solution/ time for (weakly) 
ossified bone in fish larvae?  Paraffin section tends to split due to 
the harder tissue, nice longitudinal section are hard to get.

------------------------------------------------------------------------

Tora Bardal
Overingeni?r/ Senior Engineer
NTNU Senter for fiskeri og havbruk /NTNU Center of Fisheries and Aquaculture
NTNU, 7491 Trondheim


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



-- 
The Sunday Times Scottish University of the Year 2009/2010
The University of Stirling is a charity registered in Scotland, 
 number SC 011159.


From nerosiri <@t> yahoo.co.uk  Wed Jun 15 07:49:24 2011
From: nerosiri <@t> yahoo.co.uk (iris oren)
Date: Wed Jun 15 07:49:32 2011
Subject: [Histonet] mouse-on-mouse kit and non-specific staining
Message-ID: <330289.54336.qm@web29704.mail.ird.yahoo.com>

Hi, 

I am using a mouse monoclonal antibody on PFA-fixed mouse brain slices, and the 
Vector mouse-on-mouse kit. My background with the provided avidin-biotyn is 
ridiculously high even after blocking endogenous biotyn. However, I get an 
overall low background using an Alexa-conjugated goat anti-mouse (with the 
slices pre-treated in Normal Goat Serum). The problem is that in the control 
sections with no primary, I still get staining of isolated cells.
Does anyone have any thoughts/experience/recommendation with using an Alexa 
conjugated anti mouse IgG secondary on mouse tissue (with a mouse-on-mouse 
block)?

Many thanks in advance
Iris
From sgoebel <@t> mirnarx.com  Wed Jun 15 08:59:21 2011
From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com)
Date: Wed Jun 15 08:59:26 2011
Subject: [Histonet] Keeping Histo room floor clean?
In-Reply-To: <54B2B63A-3054-414A-9D4C-97241B2505E7@imagesbyhopper.com>
References: 
	<54B2B63A-3054-414A-9D4C-97241B2505E7@imagesbyhopper.com>
Message-ID: 

You can buy this type of thing too if you aren't the McGiver type.  For
instance...from American Mastertech item # CPW04200E

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
histotech@imagesbyhopper.com
Sent: Tuesday, June 14, 2011 7:50 PM
To: JR R
Cc: 
Subject: Re: [Histonet] Keeping Histo room floor clean?

We don't keep it off the floor, but do use a wide-bladed putty knife
attached to a mop handle to scrape the residual wax off the floor. It
woks quite nicely and doesn't remove the actual floor wax like a razor
blade scrapper would.

Michelle

Sent from my iPhone

On Jun 14, 2011, at 6:44 PM, JR R  wrote:

> 
> We keep getting a lot of paraffin on the floor of one histo
room--especially around the microtome and the embedding station.
> 
> Short of laying down a tarp, what do folks do keep wax off of the
floor?
> 
> Thanks,
> 
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
>
_______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From christiegowan <@t> msn.com  Wed Jun 15 09:21:15 2011
From: christiegowan <@t> msn.com (CHRISTIE GOWAN)
Date: Wed Jun 15 09:21:23 2011
Subject: [Histonet] Keeping Histo room floor clean?
In-Reply-To: 
References: ,
	<54B2B63A-3054-414A-9D4C-97241B2505E7@imagesbyhopper.com>,
	
Message-ID: 


We use a razor blade scraper but we turn it around and use the dull side.
 

> Date: Wed, 15 Jun 2011 08:59:21 -0500
> From: sgoebel@mirnarx.com
> To: histotech@imagesbyhopper.com; rosenfeldtek@hotmail.com
> Subject: RE: [Histonet] Keeping Histo room floor clean?
> CC: histonet@lists.utsouthwestern.edu
> 
> You can buy this type of thing too if you aren't the McGiver type. For
> instance...from American Mastertech item # CPW04200E
> 
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
> 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> histotech@imagesbyhopper.com
> Sent: Tuesday, June 14, 2011 7:50 PM
> To: JR R
> Cc: 
> Subject: Re: [Histonet] Keeping Histo room floor clean?
> 
> We don't keep it off the floor, but do use a wide-bladed putty knife
> attached to a mop handle to scrape the residual wax off the floor. It
> woks quite nicely and doesn't remove the actual floor wax like a razor
> blade scrapper would.
> 
> Michelle
> 
> Sent from my iPhone
> 
> On Jun 14, 2011, at 6:44 PM, JR R  wrote:
> 
> > 
> > We keep getting a lot of paraffin on the floor of one histo
> room--especially around the microtome and the embedding station.
> > 
> > Short of laying down a tarp, what do folks do keep wax off of the
> floor?
> > 
> > Thanks,
> > 
> > Jerry Ricks
> > Research Scientist
> > University of Washington
> > Department of Pathology
> >
> _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From dhewitt <@t> hvhs.org  Wed Jun 15 09:23:54 2011
From: dhewitt <@t> hvhs.org (DANIEL HEWITT)
Date: Wed Jun 15 09:24:26 2011
Subject: [Histonet] Keeping Histo room floor clean?
In-Reply-To: 
References: ,
	<54B2B63A-3054-414A-9D4C-97241B2505E7@imagesbyhopper.com>,
	
	
Message-ID: <7DDB5AB36CBC574D8D680806E7BBE58B9B73A7@MX-HVB-02.hvhs.org>

We us a scraper I bought at Home Depot called the floor buster.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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solely those of the author and do not necessarily represent those of
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message cannot be guaranteed on the internet.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CHRISTIE
GOWAN
Sent: Wednesday, June 15, 2011 10:21 AM
To: sgoebel@mirnarx.com; histotech@imagesbyhopper.com;
rosenfeldtek@hotmail.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Keeping Histo room floor clean?


We use a razor blade scraper but we turn it around and use the dull
side.
 

> Date: Wed, 15 Jun 2011 08:59:21 -0500
> From: sgoebel@mirnarx.com
> To: histotech@imagesbyhopper.com; rosenfeldtek@hotmail.com
> Subject: RE: [Histonet] Keeping Histo room floor clean?
> CC: histonet@lists.utsouthwestern.edu
> 
> You can buy this type of thing too if you aren't the McGiver type. For
> instance...from American Mastertech item # CPW04200E
> 
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
> 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> histotech@imagesbyhopper.com
> Sent: Tuesday, June 14, 2011 7:50 PM
> To: JR R
> Cc: 
> Subject: Re: [Histonet] Keeping Histo room floor clean?
> 
> We don't keep it off the floor, but do use a wide-bladed putty knife
> attached to a mop handle to scrape the residual wax off the floor. It
> woks quite nicely and doesn't remove the actual floor wax like a razor
> blade scrapper would.
> 
> Michelle
> 
> Sent from my iPhone
> 
> On Jun 14, 2011, at 6:44 PM, JR R  wrote:
> 
> > 
> > We keep getting a lot of paraffin on the floor of one histo
> room--especially around the microtome and the embedding station.
> > 
> > Short of laying down a tarp, what do folks do keep wax off of the
> floor?
> > 
> > Thanks,
> > 
> > Jerry Ricks
> > Research Scientist
> > University of Washington
> > Department of Pathology
> >
> _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Barbara.Crill <@t> LPNT.net  Wed Jun 15 09:44:37 2011
From: Barbara.Crill <@t> LPNT.net (Barbara.Crill@LPNT.net)
Date: Wed Jun 15 09:44:42 2011
Subject: [Histonet] histotechnologist open position
Message-ID: <7DA79EBDBD92BF408EF392413737878D393F81F165@NADCWPMSGCMS01.hca.corpad.net>

Danville Regional Medical Center located in Danville, VA has an opening for a histotechnologist.
You may apply on line:
http://lifepointhealthcontent.com/custompage.asp?PageName=danvilletaleo

For Further information please contact our human resources department
434.799.4552 or 434.799.3719


Job Description
Histologist-112870 Danville Regional Medical Center
Description

Performs routine and assigned histology and pathology functions.  Processes, prepared, cuts, mounts, and stains tissue specimens from patients of all age groups, including adults, adolescents, pediatrics and infants to provide high quality anatomic pathology services in a cost efficient manner.
Qualifications

Baccalaureate degree AND/OR completion of technical training school in Histology.  HTL (ASCP) or eligible
Job
 Laboratory
Primary Location
 Virginia-Danville

Schedule
 Full-time
Shift
 Day Job



ANTOINETTE CRILL, MBA,CT(ASCP)
TEAM LEADER ANATOMIC PATHOLOGY
DANVILLE REGIONAL MEDICAL CENTER
(O) 434.799.4470
(F) 434.773.6806
E-mail:  barbara.crill@LPNT.net

From mlinn <@t> med.umich.edu  Wed Jun 15 09:51:41 2011
From: mlinn <@t> med.umich.edu (Linn, Marisa)
Date: Wed Jun 15 09:51:43 2011
Subject: [Histonet] RE:  Paraffin processing and embeding of Chondrocyte
Message-ID: <28BE8337587025409267FD71069A91A64F0876@UHEXMBSPR10.umhs.med.umich.edu>

Hi Bret-
I've dealt with chondrocyte pellets a few times.  It's a pretty half-assed technique, but works pretty well if the pellet is relatively solid...if it's not, maybe someone else can offer a useful suggestion :)
I was taught to use a piece of lens paper; pre-fold it in half several times, and drop the pellet along one of the folds and close the thing up.  If you trap an edge or two of the paper in the cassette lid (or put a sponge in), the paper shouldn't be able to unfold.  When the time comes to embed, keep that paraffin nice and melty.  
For sectioning...check frequently under a scope.   
Good luck,
Marisa

-----Original Message-----

Message: 19
Date: Wed, 15 Jun 2011 02:52:29 +0000
From: "Clough, Bret" 
Subject: [Histonet] Paraffin processing and embeding of Chondrocyte
	pellets.
To: "Histonet list serv. (histonet@lists.utsouthwestern.edu)"
	
Message-ID:
	
Content-Type: text/plain; charset="iso-8859-1"

Hello Everyone,
 I was wondering if anyone has a protocol for paraffin embedding chondrocyte pellets that they would be willing to share with me? Any help would be greatly appreciated.

Thanks,
     Bret

Bret Clough
TAMHSC

**********************************************************
Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues 


From liz <@t> premierlab.com  Wed Jun 15 09:58:50 2011
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Wed Jun 15 09:58:55 2011
Subject: [Histonet] Paraffin processing and embeding of Chondrocyte
	pellets.
In-Reply-To: 
Message-ID: 

Bret

We have done a bit of work with these types of samples, the first thing
we do is remove the formalin and add eosin to the container for about 5
minutes.  Then we place the pellet (using a plastic disposable pipettor)
in a mesh bag and process on our tissue processor on a cycle that is set
at 7 minutes per station, we also used propar instead of xylene (3
changes).  We used to process by hand but this method works just as
well.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clough,
Bret
Sent: Tuesday, June 14, 2011 8:52 PM
To: Histonet list serv. (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Paraffin processing and embeding of Chondrocyte
pellets.

Hello Everyone,
 I was wondering if anyone has a protocol for paraffin embedding
chondrocyte pellets that they would be willing to share with me? Any
help would be greatly appreciated.

Thanks,
     Bret

Bret Clough
TAMHSC

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From algranth <@t> email.arizona.edu  Wed Jun 15 10:07:37 2011
From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth))
Date: Wed Jun 15 10:07:52 2011
Subject: [Histonet] Keeping Histo room floor clean?
In-Reply-To: 
References: 
Message-ID: <5A71FC71-5A70-45D0-8C06-B6A3C5B6E38E@email.arizona.edu>

I periodically use a scraper to get the wax off the floor under the microtomes and I have housekeeping strip the floors a couple of times a year. We also use a shop vac to vacuum up around the embedder and throughout the lab. We have sticky mats at the entrance to the microtome stations and at the door to the embedding room. Also Market lab makes these mats that trap paraffin and we have them throughout the lab. They come in a couple different sizes and also you can get custom sizes.
All this because we almost took out our department head one day when he went slipping over the waxy floor - before my time but I imagine it wasn't pretty.
You also just have to be aware that what we do produces waxy debris and you have to be careful as to not make such a big mess. I teach the students to be neat and to clean up after themselves - and they do!

Andi


On Jun 14, 2011, at 3:44 PM, JR R wrote:

> 
> We keep getting a lot of paraffin on the floor of one histo room--especially around the microtome and the embedding station.
> 
> Short of laying down a tarp, what do folks do keep wax off of the floor?
> 
> Thanks,
> 
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
> 		 	   		  _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From jbirkner <@t> colabserv.com  Wed Jun 15 10:08:54 2011
From: jbirkner <@t> colabserv.com (Jeff Birkner)
Date: Wed Jun 15 10:09:02 2011
Subject: [Histonet] special stain validation documentation
Message-ID: 

Just wondering how everyone is formally documenting validation of manual
special stains.  If you have a template/form to share that would be
great.  Thanks!

 

Jeffrey C. Birkner, CT(ASCP)

Manager, Pathology Laboratory Section

Collaborative Laboratory Services, L.L.C.

1005 Pennsylvania Ave, Suite 102

Ottumwa, IA  52501

641-455-5414

ORHC Extension #3538

jbirkner@colabserv.com

 

 

 

From rjbuesa <@t> yahoo.com  Wed Jun 15 10:18:13 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Jun 15 10:18:16 2011
Subject: [Histonet] decal fish larvae
In-Reply-To: <4DF8785A.3000704@bio.ntnu.no>
References: <4DF8785A.3000704@bio.ntnu.no>
Message-ID: <679314.13847.qm@web65705.mail.ac4.yahoo.com>

Try EDTA for at least 24 hours
Ren? J.

From: Tora Bardal 
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, June 15, 2011 5:16 AM
Subject: [Histonet] decal fish larvae

Hi

Does anyone have a suggestion for decal solution/ time for (weakly) ossified bone in fish larvae?? Paraffin section tends to split due to the harder tissue, nice longitudinal section are hard to get.

------------------------------------------------------------------------

Tora Bardal
Overingeni?r/ Senior Engineer
NTNU Senter for fiskeri og havbruk /NTNU Center of Fisheries and Aquaculture
NTNU, 7491 Trondheim


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From TNMayer <@t> mdanderson.org  Wed Jun 15 10:17:13 2011
From: TNMayer <@t> mdanderson.org (Mayer,Toysha N)
Date: Wed Jun 15 10:20:49 2011
Subject: [Histonet] RE: Histonet Digest, Vol 91, Issue 21;
 Keeping Histo room floor clean? (JR R)
In-Reply-To: <674cf7db-33d3-4fd2-aa54-abedc11f355d@DCPWPRTR02.mdanderson.edu>
References: <674cf7db-33d3-4fd2-aa54-abedc11f355d@DCPWPRTR02.mdanderson.edu>
Message-ID: 

I will call myself old school and say, a slide works well and can be disposed of in the broken glass box.
Now you can get your housekeeping dept to just shower scrub your floor and not put wax on it.  But the putty knife, especially on a broom or mop handle is great, and easier on the knees and back.


Toysha N. Mayer, MBA, HT (ASCP)
Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnmayer@mdanderson.org

Message: 15
Date: Tue, 14 Jun 2011 20:49:57 -0400
From: "histotech@imagesbyhopper.com" 
Subject: Re: [Histonet] Keeping Histo room floor clean?
To: JR R 
Cc: ""
	
Message-ID: <54B2B63A-3054-414A-9D4C-97241B2505E7@imagesbyhopper.com>
Content-Type: text/plain; charset=us-ascii

We don't keep it off the floor, but do use a wide-bladed putty knife attached to a mop handle to scrape the residual wax off the floor. It woks quite nicely and doesn't remove the actual floor wax like a razor blade scrapper would.

Michelle

Sent from my iPhone

On Jun 14, 2011, at 6:44 PM, JR R  wrote:

> 
> We keep getting a lot of paraffin on the floor of one histo room--especially around the microtome and the embedding station.
> 
> Short of laying down a tarp, what do folks do keep wax off of the floor?
> 
> Thanks,
> 
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
>                         _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


CONFIDENTIALITY NOTICE: This email and any attachments may contain
confidential information that is protected by law and is for the
sole use of the individuals or entities to which it is addressed.
If you are not the intended recipient, please notify the sender by
replying to this email and destroying all copies of the
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attachments is strictly prohibited. To contact Albany Medical
Center, or for a copy of our privacy practices, please visit us on
the Internet at www.amc.edu.



From BBranton <@t> sarapath.com  Wed Jun 15 10:22:46 2011
From: BBranton <@t> sarapath.com (Brian Branton)
Date: Wed Jun 15 10:22:51 2011
Subject: [Histonet] P5049-15 - Anyone interested buying some Pipette tips
	for Oxford pipetters 
Message-ID: 

Hello HistoNetters,

 

I was cleaning out a cabinet in the Laboratory, and came across 8 bags
of Allegiance S/P AccuTip Nonfiltered Pipett Tips (# P5049-15). They fit
a Oxford P-3000 or P-7000 pipetter. We no longer have the pipetter for
these tips. I am selling all eight bags for $20.00 plus shipping.

 

Would anyone be interested?

 

Brian Branton 
Purchasing Agent 

SaraPath Diagnostics

Sarasota Pathology

Sarasota Professional Enterprises II 
(941) 362-8963 
(941) 362-8964 FAX 

 

 

From LTurner1 <@t> seton.org  Wed Jun 15 10:52:38 2011
From: LTurner1 <@t> seton.org (Turner, Leandra)
Date: Wed Jun 15 10:52:42 2011
Subject: [Histonet] RE: Histonet Digest, Vol 91, Issue 21;
	Keeping Histo room floor clean? (JR R)
In-Reply-To: 
References: <674cf7db-33d3-4fd2-aa54-abedc11f355d@DCPWPRTR02.mdanderson.edu>
	
Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB010AB82F@AUSEX2VS1.seton.org>

Hi,
We use sticky floor mats from market lab that help to collect any stray
paraffin.  We use a scraper from market lab, it has a long handle so you
don't have to stoop over, to scrap up any paraffin that did not land on
the maps.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Mayer,Toysha N
Sent: Wednesday, June 15, 2011 10:17 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Histonet Digest, Vol 91, Issue 21; Keeping Histo
room floor clean? (JR R)

I will call myself old school and say, a slide works well and can be
disposed of in the broken glass box.
Now you can get your housekeeping dept to just shower scrub your floor
and not put wax on it.  But the putty knife, especially on a broom or
mop handle is great, and easier on the knees and back.


Toysha N. Mayer, MBA, HT (ASCP)
Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnmayer@mdanderson.org

Message: 15
Date: Tue, 14 Jun 2011 20:49:57 -0400
From: "histotech@imagesbyhopper.com" 
Subject: Re: [Histonet] Keeping Histo room floor clean?
To: JR R 
Cc: ""
	
Message-ID: <54B2B63A-3054-414A-9D4C-97241B2505E7@imagesbyhopper.com>
Content-Type: text/plain; charset=us-ascii

We don't keep it off the floor, but do use a wide-bladed putty knife
attached to a mop handle to scrape the residual wax off the floor. It
woks quite nicely and doesn't remove the actual floor wax like a razor
blade scrapper would.

Michelle

Sent from my iPhone

On Jun 14, 2011, at 6:44 PM, JR R  wrote:

> 
> We keep getting a lot of paraffin on the floor of one histo
room--especially around the microtome and the embedding station.
> 
> Short of laying down a tarp, what do folks do keep wax off of the
floor?
> 
> Thanks,
> 
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
>
_______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


CONFIDENTIALITY NOTICE: This email and any attachments may contain
confidential information that is protected by law and is for the
sole use of the individuals or entities to which it is addressed.
If you are not the intended recipient, please notify the sender by
replying to this email and destroying all copies of the
communication and attachments. Further use, disclosure, copying,
distribution of, or reliance upon the contents of this email and
attachments is strictly prohibited. To contact Albany Medical
Center, or for a copy of our privacy practices, please visit us on
the Internet at www.amc.edu.



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From LTurner1 <@t> seton.org  Wed Jun 15 10:55:39 2011
From: LTurner1 <@t> seton.org (Turner, Leandra)
Date: Wed Jun 15 10:55:43 2011
Subject: [Histonet] RE: Histonet Digest, Vol 91, Issue 21;
	Keeping Histo room floor clean? (JR R)
In-Reply-To: <3D79F47DC92B204F9E5D35C885DFC5CB010AB82F@AUSEX2VS1.seton.org>
References: <674cf7db-33d3-4fd2-aa54-abedc11f355d@DCPWPRTR02.mdanderson.edu>
	<3D79F47DC92B204F9E5D35C885DFC5CB010AB82F@AUSEX2VS1.seton.org>
Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB010AB830@AUSEX2VS1.seton.org>

Ohh and we also got housekeeping to give us a broom and long handled
dust pan

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Turner,
Leandra
Sent: Wednesday, June 15, 2011 10:53 AM
To: Mayer,Toysha N; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Histonet Digest, Vol 91, Issue 21;Keeping
Histo room floor clean? (JR R)

Hi,
We use sticky floor mats from market lab that help to collect any stray
paraffin.  We use a scraper from market lab, it has a long handle so you
don't have to stoop over, to scrap up any paraffin that did not land on
the maps.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Mayer,Toysha N
Sent: Wednesday, June 15, 2011 10:17 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Histonet Digest, Vol 91, Issue 21; Keeping Histo
room floor clean? (JR R)

I will call myself old school and say, a slide works well and can be
disposed of in the broken glass box.
Now you can get your housekeeping dept to just shower scrub your floor
and not put wax on it.  But the putty knife, especially on a broom or
mop handle is great, and easier on the knees and back.


Toysha N. Mayer, MBA, HT (ASCP)
Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnmayer@mdanderson.org

Message: 15
Date: Tue, 14 Jun 2011 20:49:57 -0400
From: "histotech@imagesbyhopper.com" 
Subject: Re: [Histonet] Keeping Histo room floor clean?
To: JR R 
Cc: ""
	
Message-ID: <54B2B63A-3054-414A-9D4C-97241B2505E7@imagesbyhopper.com>
Content-Type: text/plain; charset=us-ascii

We don't keep it off the floor, but do use a wide-bladed putty knife
attached to a mop handle to scrape the residual wax off the floor. It
woks quite nicely and doesn't remove the actual floor wax like a razor
blade scrapper would.

Michelle

Sent from my iPhone

On Jun 14, 2011, at 6:44 PM, JR R  wrote:

> 
> We keep getting a lot of paraffin on the floor of one histo
room--especially around the microtome and the embedding station.
> 
> Short of laying down a tarp, what do folks do keep wax off of the
floor?
> 
> Thanks,
> 
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
>
_______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


CONFIDENTIALITY NOTICE: This email and any attachments may contain
confidential information that is protected by law and is for the
sole use of the individuals or entities to which it is addressed.
If you are not the intended recipient, please notify the sender by
replying to this email and destroying all copies of the
communication and attachments. Further use, disclosure, copying,
distribution of, or reliance upon the contents of this email and
attachments is strictly prohibited. To contact Albany Medical
Center, or for a copy of our privacy practices, please visit us on
the Internet at www.amc.edu.



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From techmana12 <@t> yahoo.com  Wed Jun 15 10:41:19 2011
From: techmana12 <@t> yahoo.com (Dorothy Glass)
Date: Wed Jun 15 11:36:52 2011
Subject: [Histonet] (no subject)
Message-ID: <63898.94531.qm@web114519.mail.gq1.yahoo.com>

Dear Histonets, 
How is it possible to validate a antibody on the Ventana Ultra or XT, when the 
antibodys afre prediluted or made into a prep-kit?
From liz <@t> premierlab.com  Wed Jun 15 11:42:07 2011
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Wed Jun 15 11:42:11 2011
Subject: [Histonet] (no subject)
In-Reply-To: <63898.94531.qm@web114519.mail.gq1.yahoo.com>
Message-ID: 

Dorothy

I would assume that you would just run the validation as you would any
other antibody that is not a pre dilute.  Run the antibody protocol on
the number of tissues that you normally do to validate, evaluate and
record the results.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy
Glass
Sent: Wednesday, June 15, 2011 9:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Dear Histonets, 
How is it possible to validate a antibody on the Ventana Ultra or XT,
when the 
antibodys afre prediluted or made into a prep-kit?
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From TMcNemar <@t> lmhealth.org  Wed Jun 15 12:51:59 2011
From: TMcNemar <@t> lmhealth.org (Tom McNemar)
Date: Wed Jun 15 12:52:08 2011
Subject: [Histonet] Source for p16 antibody... 
Message-ID: 

Hello all,

I'm looking for p16 antibody for use on the Benchmark XT and was wondering what others are using and where they are getting it.  Any input appreciated.  Thanks.


Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar@lmhealth.org
www.LMHealth.org


________________________________
This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
From CIngles <@t> uwhealth.org  Wed Jun 15 12:52:58 2011
From: CIngles <@t> uwhealth.org (Ingles Claire )
Date: Wed Jun 15 12:54:44 2011
Subject: [Histonet] RE: Retirement
References: <4D14F0FC9316DD41972D5F03C070908B051DF759@nmdamailsvr.nmda.ad.nmsu.edu>
	<5A2BD13465E061429D6455C8D6B40E390EBF65E9F7@IBMB7Exchange.digestivespecialists.com>
Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A6FE@UWHC-MAIL2.uwhis.hosp.wisc.edu>

Try about 30 (years)!
Claire

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Blazek, Linda
Sent: Tue 6/14/2011 2:25 PM
To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Retirement



7 years compared to 8 months is gigantically significant.





From wbenton <@t> cua.md  Wed Jun 15 13:02:46 2011
From: wbenton <@t> cua.md (Walter Benton)
Date: Wed Jun 15 13:03:44 2011
Subject: [Histonet] RE: Source for p16 antibody... 
In-Reply-To: 
References: 
Message-ID: <0B8979A204680A42B93A52B486088CD91D6EC7CF57@CUAEXH1.GCU-MD.local>

 MTM is the only company that can sell the antibody.
http://www.mtmlabs.com/us/index.php

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wbenton@cua.md
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar [TMcNemar@lmhealth.org]
Sent: Wednesday, June 15, 2011 1:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Source for p16 antibody...

Hello all,

I'm looking for p16 antibody for use on the Benchmark XT and was wondering what others are using and where they are getting it.  Any input appreciated.  Thanks.


Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar@lmhealth.org
www.LMHealth.org


________________________________
This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law.  If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy.

From histotech <@t> imagesbyhopper.com  Wed Jun 15 13:10:27 2011
From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com)
Date: Wed Jun 15 13:10:42 2011
Subject: [Histonet] (no subject)
In-Reply-To: <63898.94531.qm@web114519.mail.gq1.yahoo.com>
References: <63898.94531.qm@web114519.mail.gq1.yahoo.com>
Message-ID: 

Dorothy,

Is your question related to the fact that a protocol will have the "currently active" lot number and you want to use a different lot number?  Or is this a brand new antibody that has not been in use before and therefore has not ever been validated?

Michelle


On Jun 15, 2011, at 11:41 AM, Dorothy Glass  wrote:

> Dear Histonets, 
> How is it possible to validate a antibody on the Ventana Ultra or XT, when the 
> antibodys afre prediluted or made into a prep-kit?
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From Loralee_Mcmahon <@t> URMC.Rochester.edu  Wed Jun 15 13:15:53 2011
From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A)
Date: Wed Jun 15 13:16:11 2011
Subject: [Histonet] RE: Source for p16 antibody... 
In-Reply-To: 
References: 
Message-ID: 

MTM labs is the only place to get it IVD. 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar [TMcNemar@lmhealth.org]
Sent: Wednesday, June 15, 2011 1:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Source for p16 antibody...

Hello all,

I'm looking for p16 antibody for use on the Benchmark XT and was wondering what others are using and where they are getting it.  Any input appreciated.  Thanks.


Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar@lmhealth.org
www.LMHealth.org


________________________________
This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From freidac <@t> sbcglobal.net  Wed Jun 15 14:40:40 2011
From: freidac <@t> sbcglobal.net (Freida Carson)
Date: Wed Jun 15 14:40:52 2011
Subject: [Histonet] Downsizing my library
Message-ID: <511757.73780.qm@web82503.mail.mud.yahoo.com>

I am downsizing my library and have the following books, mostly out of print, for sale.? If you are interested, make an offerf or any of them with enough to cover postage also. I hate to just throw them away. Please do this to my private email and not on histonet.

Carson:? Histotechnology: A Self-Instructional Text - 2nd edition.
2007
Bancroft and Cook: Manual of Histological Techniques and their Diagnostic Application. 1994

Montgomery: Health and Safety Guidelines for the Laboratory, 1995.

Dapson and Dapson: Hazardous Materials in the Histopathology Laboratory. 3rd ed, 1995

Wheater, Burkitt and Daniels: Functional Histology. 1987.

Lillie and Fullmer: Histopathologic Technic and Practical Histochemistry. 1976

Kiernan: Histological & Histochemical Methods. 1999.

Vacca: Laboratory Manual of Histochemistry.? 1985

Bancroft and Stevens: Theory and Practice of Histological Techniques. 1996


Freida Carson 

From rjbuesa <@t> yahoo.com  Wed Jun 15 15:20:04 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Jun 15 15:20:21 2011
Subject: [Histonet] (no subject)
In-Reply-To: <63898.94531.qm@web114519.mail.gq1.yahoo.com>
References: <63898.94531.qm@web114519.mail.gq1.yahoo.com>
Message-ID: <120354.13670.qm@web65709.mail.ac4.yahoo.com>

Validating an Ab does not involve testing different concentrations, just a known (+) tissue to be evaluated by the pathologist that should compare the results with other tissues already reacted with the same Ab and protocol.
If the pathologist accepts the results, you have your validation.
Ren? J.

From: Dorothy Glass 
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, June 15, 2011 11:41 AM
Subject: [Histonet] (no subject)

Dear Histonets, 
How is it possible to validate a antibody on the Ventana Ultra or XT, when the 
antibodys afre prediluted or made into a prep-kit?
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From nelsonrnch <@t> verizon.net  Wed Jun 15 18:47:28 2011
From: nelsonrnch <@t> verizon.net (SHANE NELSON)
Date: Wed Jun 15 18:47:31 2011
Subject: [Histonet] Library-Freida Carson
Message-ID: <500393.11109.qm@web84303.mail.re1.yahoo.com>

Mrs. Carson,
Would it be possible to e-mail me the cost of these books? I would greatly 
appreciate it and I am very interested.



Carson:? Histotechnology: A Self-Instructional Text - 2nd edition.
2007

Bancroft and Cook: Manual of Histological Techniques and their Diagnostic 
Application. 1994


Wheater, Burkitt and Daniels: Functional Histology. 1987.

Lillie and Fullmer: Histopathologic Technic and Practical Histochemistry. 1976

Vacca: Laboratory Manual of Histochemistry.? 1985

Bancroft and Stevens: Theory and Practice of Histological Techniques. 1996


THANK YOU,
?
PATTI RUBEN-NELSON? H.T.(ASCP) 
PNP LABORATORY CONSULTANTS
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761 
nelsonrnch@verizon.net
From MLashus <@t> pathgroup.com  Thu Jun 16 08:11:10 2011
From: MLashus <@t> pathgroup.com (Mighnon Lashus)
Date: Thu Jun 16 08:17:25 2011
Subject: [Histonet] New iView Blue Plus Kit from Ventana
Message-ID: <197CD0B02A81F94994A285C59C8AE05C0789963B69@pgnexchange.pathgroup.com>

Hello everyone,
I am working up new protocols for Kappa and Lambda ISH using the new probes and the iView Blue Plus detection Kit from Ventana.  Since the probes are ASR, Ventana says they cannot help.  I am performing the stains on the Ventana XT stainer.  I have tried several times with less than optimal results; any help here would be appreciated.  Thanks

Mighnon Lashus, HT (ASCP)
PathGroup Chattanooga
4071 S. Access Road, Suite 107
Chattanooga, TN  37406
Phone:  423-493-0207
Fax:  423-493-0208
Email:  mlashus@pathgroup.com


________________________________
Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you
From LSebree <@t> uwhealth.org  Thu Jun 16 08:28:26 2011
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Thu Jun 16 08:28:35 2011
Subject: [Histonet] New iView Blue Plus Kit from Ventana
In-Reply-To: <197CD0B02A81F94994A285C59C8AE05C0789963B69@pgnexchange.pathgroup.com>
References: <197CD0B02A81F94994A285C59C8AE05C0789963B69@pgnexchange.pathgroup.com>
Message-ID: 

Hi Mighnon,

Marc Shaeffer sent out protocols via Histonet not long ago for K, L, and
EBER; try him (mshaeffer@cox.net) 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mighnon
Lashus
Sent: Thursday, June 16, 2011 8:11 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] New iView Blue Plus Kit from Ventana

Hello everyone,
I am working up new protocols for Kappa and Lambda ISH using the new
probes and the iView Blue Plus detection Kit from Ventana.  Since the
probes are ASR, Ventana says they cannot help.  I am performing the
stains on the Ventana XT stainer.  I have tried several times with less
than optimal results; any help here would be appreciated.  Thanks

Mighnon Lashus, HT (ASCP)
PathGroup Chattanooga
4071 S. Access Road, Suite 107
Chattanooga, TN  37406
Phone:  423-493-0207
Fax:  423-493-0208
Email:  mlashus@pathgroup.com


________________________________
Important Notice: This e-mail is intended for the use of the person to
whom it is addressed and may contain information that is privileged and
confidential. If you are not the intended recipient, any disclosure,
copying, distribution, or use of the contents of this message is
strictly prohibited. If you have received this e-mail in error, please
destroy this message and contact the Security Officer at PathGroup, Inc
immediately at 615-562-9255. Thank you
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From c.tague <@t> pathologyarts.com  Thu Jun 16 09:34:13 2011
From: c.tague <@t> pathologyarts.com (Curt Tague)
Date: Thu Jun 16 09:34:22 2011
Subject: [Histonet] IHC verification forms
Message-ID: <019901cc2c32$76a67870$63f36950$@tague@pathologyarts.com>

Please help me understand, do we need to verify antibodies or validate them,
or is it essentially the same thing? Also, can I please get a couple
examples of the forms you might be using, perhaps email me a copy???

I'm still new to the IHC world and trying to get things inline as they need
to be.

 

Thanks,

curt

From settembr <@t> umdnj.edu  Thu Jun 16 10:41:37 2011
From: settembr <@t> umdnj.edu (Settembre, Dana)
Date: Thu Jun 16 10:41:45 2011
Subject: [Histonet] IHC verification forms
In-Reply-To: <019901cc2c32$76a67870$63f36950$%tague@pathologyarts.com>
References: <019901cc2c32$76a67870$63f36950$%tague@pathologyarts.com>
Message-ID: 

Validate.
Dana Settembre
University Hospital - UMDNJ
Newark, NJ  07103

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Tague
Sent: Thursday, June 16, 2011 10:34 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC verification forms

Please help me understand, do we need to verify antibodies or validate them,
or is it essentially the same thing? Also, can I please get a couple
examples of the forms you might be using, perhaps email me a copy???

I'm still new to the IHC world and trying to get things inline as they need
to be.

 

Thanks,

curt

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Allison_Scott <@t> hchd.tmc.edu  Thu Jun 16 10:51:29 2011
From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D)
Date: Thu Jun 16 10:51:32 2011
Subject: [Histonet] Surgipath VCP cassette printer
Message-ID: <1872B4A455B7974391609AD8034C79FC026DFE0C@LBEXCH01.hchd.local>

We have a old surgipath VCP cassette printer that is not working due to
the print head going out.  We have had this unit for some time. Leica
has since taken over Surgipath, and they no longer support this
particular unit.  Does anyone know where I can get a replacement
printhead for this unit. This is the printer that our residents use for
making additional cassettes.  We have a Leica IPC printer that we make
the bulk of the cassettes on,and the resients are not allowed to use
this one.  Any help in this will be greatly appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
CONFIDENTIALITY NOTICE:
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To the extent the information in this e-mail and any attachments contain 
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and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
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From freckles9660 <@t> yahoo.com  Thu Jun 16 14:32:16 2011
From: freckles9660 <@t> yahoo.com (Karla Arrington)
Date: Thu Jun 16 14:32:20 2011
Subject: [Histonet] HTL exam
Message-ID: <953560.67364.qm@web112619.mail.gq1.yahoo.com>

Histonetters:

I have a co-hort that is going to be taking the HTL exam shortly.? As far as 
studying goes, what content of IHC does
he need to know... example IHC stains.? The list just states IHC stains.? There 
are lots of them so I would like some
information as to how much of stains are on the exam if anyone can enlighte 
me.... 


Thanks!!
Karla 
From JMacDonald <@t> mtsac.edu  Thu Jun 16 14:55:24 2011
From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald)
Date: Thu Jun 16 14:55:29 2011
Subject: [Histonet] HTL exam
In-Reply-To: <953560.67364.qm@web112619.mail.gq1.yahoo.com>
Message-ID: 

Have you looked at the examination guidelines posted at 
http://www.ascp.org/pdf/BOR-PDFs/Guidelines/ExaminationContentGuidelineHT.aspx




Karla Arrington  
Sent by: histonet-bounces@lists.utsouthwestern.edu
06/16/2011 12:33 PM

To
Histonet 
cc

Subject
[Histonet] HTL exam






Histonetters:

I have a co-hort that is going to be taking the HTL exam shortly.  As far 
as 
studying goes, what content of IHC does
he need to know... example IHC stains.  The list just states IHC stains.  
There 
are lots of them so I would like some
information as to how much of stains are on the exam if anyone can 
enlighte 
me.... 


Thanks!!
Karla 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From rjbuesa <@t> yahoo.com  Thu Jun 16 15:24:56 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Jun 16 15:25:00 2011
Subject: [Histonet] HTL exam
In-Reply-To: <953560.67364.qm@web112619.mail.gq1.yahoo.com>
References: <953560.67364.qm@web112619.mail.gq1.yahoo.com>
Message-ID: <652935.53139.qm@web65716.mail.ac4.yahoo.com>

I believe that "IHC stains" (by the way, they are not stains but detectable immunohistochemical reactions) refers to the method itself and its methodology.
Ren? J.

From: Karla Arrington 
To: Histonet 
Sent: Thursday, June 16, 2011 3:32 PM
Subject: [Histonet] HTL exam

Histonetters:

I have a co-hort that is going to be taking the HTL exam shortly.? As far as 
studying goes, what content of IHC does
he need to know... example IHC stains.? The list just states IHC stains.? There 
are lots of them so I would like some
information as to how much of stains are on the exam if anyone can enlighte 
me.... 


Thanks!!
Karla
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From freidac <@t> sbcglobal.net  Thu Jun 16 15:30:19 2011
From: freidac <@t> sbcglobal.net (Freida Carson)
Date: Thu Jun 16 15:30:25 2011
Subject: [Histonet] books
Message-ID: <819411.58346.qm@web82507.mail.mud.yahoo.com>

Thanks to everyone.? The books are sold and now I will have room for something else maybe.

Freida Carson
From rceades <@t> gmail.com  Thu Jun 16 17:15:01 2011
From: rceades <@t> gmail.com (Eric Eades)
Date: Thu Jun 16 17:15:04 2011
Subject: [Histonet] HTL exam
In-Reply-To: <652935.53139.qm@web65716.mail.ac4.yahoo.com>
References: <953560.67364.qm@web112619.mail.gq1.yahoo.com>
	<652935.53139.qm@web65716.mail.ac4.yahoo.com>
Message-ID: 

Ren? is correct.  IHC stains are named according to the primary antibody
used, but it would be useless to ask questions about individual antibodies
because new ones are continuously developed.  Study the general method and
how it might vary (pretreatments, detection systems, chromogens.)

-Eric

On Thu, Jun 16, 2011 at 1:24 PM, Rene J Buesa  wrote:

> I believe that "IHC stains" (by the way, they are not stains but detectable
> immunohistochemical reactions) refers to the method itself and its
> methodology.
> Ren? J.
>
> From: Karla Arrington 
> To: Histonet 
> Sent: Thursday, June 16, 2011 3:32 PM
> Subject: [Histonet] HTL exam
>
> Histonetters:
>
> I have a co-hort that is going to be taking the HTL exam shortly.  As far
> as
> studying goes, what content of IHC does
> he need to know... example IHC stains.  The list just states IHC stains.
> There
> are lots of them so I would like some
> information as to how much of stains are on the exam if anyone can enlighte
> me....
>
>
> Thanks!!
> Karla
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From Jayne.Halli <@t> avera.org  Fri Jun 17 09:20:57 2011
From: Jayne.Halli <@t> avera.org (Jayne Halli)
Date: Fri Jun 17 09:21:07 2011
Subject: [Histonet] Re p16 antibody
Message-ID: 

Tom, I have the Dako Autostainer Lin 48 and use the platform Flex
envision kit, but I get my p16 from Cintec it is the only IVD I could
find. They are very helpful in helping you work it up with any platform
you may be using.

 

Jayne Halli HT,ASCP,QIHC

Avera McKennan Histology 

800 East 21st street

Sioux Falls, SD 57117

(605)322-7142

 

my new e-mail address is jayne.halli@avera.org

 



-----------------------------------------
Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters.
From amber.mckenzie <@t> gastrodocs.net  Fri Jun 17 09:26:45 2011
From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie)
Date: Fri Jun 17 09:26:23 2011
Subject: [Histonet] gross room QC form
In-Reply-To: 
References: 
Message-ID: <03C921A1EAF7F541B16543F6EC6A4B37043604DC@giamail2.Gia.com>

Does anyone use a QC form for the Gross Room?  I was thinking of using
one to keep up with the cleaning of the grossing table...any
suggestions? 




From jesus.w.hdz <@t> gmail.com  Fri Jun 17 10:36:31 2011
From: jesus.w.hdz <@t> gmail.com (Jesus Hernandez)
Date: Fri Jun 17 10:36:35 2011
Subject: [Histonet] DAPI staining methacrylate sections
Message-ID: 

Hi everyone,

I am in a rush to finish my master's thesis, but I needed to know
exactly if it was possible to stain methacrylate embedded sections
with DAPI and if so does anyone have a method for this? I am currently
looking at cell distribution within these scaffold constructs that I
have produced. The duration of the study is for 4 weeks. I am using
HEPM (Human Embryonic Palatal Mesenchymal) cells. After staining, I
will analyze the number of cells within this scaffold construct to
count / estimate how many cells actually survived as well as the
distance they were able to migrate using Bioquant. If anyone has an
answer please do not hesitate to respond. Thank you.


Thanks,

Jesse Hernandez

University of Texas - San Antonio
One UTSA Circle, San Antonio TX 78249
Department of Biomedical Engineering

From billodonnell <@t> catholichealth.net  Fri Jun 17 11:00:33 2011
From: billodonnell <@t> catholichealth.net (O'Donnell, Bill)
Date: Fri Jun 17 11:00:52 2011
Subject: [Histonet] Retirement
In-Reply-To: 
References: 
Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>


 OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC 
Senior Histologist/Safety Officer
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.



 


From robinsoc <@t> mercyhealth.com  Fri Jun 17 11:13:27 2011
From: robinsoc <@t> mercyhealth.com (Cynthia Robinson)
Date: Fri Jun 17 11:13:34 2011
Subject: [Histonet] Retirement
In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>
References: 
	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>
Message-ID: <4DFB36D7.D67D.00AF.1@mercyhealth.com>

Bill,

I am about the same age as you are and would like the same type of locale. Since there are so many of us in this field due to retire in the next 15-18 years maybe we should consider investing in a Histotech Retirement Community. Specimens could be shipped to us for processing and staining. We could set up microtome stations in the common area and we could get together and talk and cut at the same time and do it in a wonderful climate with nice views and of course....at our own pace which will be relaxed and more of a shuffle that a full out sprint. Anyone have any suggestions for naming such a paradise? 

Ok...I'm just getting old and it is Friday..so hope you appreciate my humor.

Have a good weekend everyone!
>From here in the Midwest, where waterfront property is in abundance along the Mighty MO

Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc@mercyhealth.com


>>> "O'Donnell, Bill"  6/17/2011 11:00 AM >>>

 OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com 

William (Bill) O'Donnell, HT (ASCP) QIHC 
Senior Histologist/Safety Officer
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.



 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From rjbuesa <@t> yahoo.com  Fri Jun 17 11:37:36 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Fri Jun 17 11:37:40 2011
Subject: [Histonet] Retirement
In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>
References: 
	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>
Message-ID: <260445.63330.qm@web65705.mail.ac4.yahoo.com>

I Guam out of the "wish list"?
Ren? J

From: "O'Donnell, Bill" 
To: histonet@lists.utsouthwestern.edu
Sent: Friday, June 17, 2011 12:00 PM
Subject: [Histonet] Retirement


OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC 
Senior Histologist/Safety Officer
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.






_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From rjbuesa <@t> yahoo.com  Fri Jun 17 11:39:33 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Fri Jun 17 11:39:37 2011
Subject: [Histonet] Retirement
In-Reply-To: <4DFB36D7.D67D.00AF.1@mercyhealth.com>
References: 
	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>
	<4DFB36D7.D67D.00AF.1@mercyhealth.com>
Message-ID: <454772.43489.qm@web65712.mail.ac4.yahoo.com>

How about: "Center?of Casual histology"?
Ren? J.

From: Cynthia Robinson 
To: Bill O'Donnell ; histonet@lists.utsouthwestern.edu
Sent: Friday, June 17, 2011 12:13 PM
Subject: Re: [Histonet] Retirement

Bill,

I am about the same age as you are and would like the same type of locale. Since there are so many of us in this field due to retire in the next 15-18 years maybe we should consider investing in a Histotech Retirement Community. Specimens could be shipped to us for processing and staining. We could set up microtome stations in the common area and we could get together and talk and cut at the same time and do it in a wonderful climate with nice views and of course....at our own pace which will be relaxed and more of a shuffle that a full out sprint. Anyone have any suggestions for naming such a paradise? 

Ok...I'm just getting old and it is Friday..so hope you appreciate my humor.

Have a good weekend everyone!
>From here in the Midwest, where waterfront property is in abundance along the Mighty MO

Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc@mercyhealth.com


>>> "O'Donnell, Bill"  6/17/2011 11:00 AM >>>

OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com 

William (Bill) O'Donnell, HT (ASCP) QIHC 
Senior Histologist/Safety Officer
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.






_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From NMP <@t> stowers.org  Fri Jun 17 11:41:42 2011
From: NMP <@t> stowers.org (Marsh, Nannette)
Date: Fri Jun 17 11:41:51 2011
Subject: [Histonet] Retirement
In-Reply-To: <4DFB36D7.D67D.00AF.1@mercyhealth.com>
References: 
	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>
	<4DFB36D7.D67D.00AF.1@mercyhealth.com>
Message-ID: <2C40E43D1F7A56408C4463FD245DDDF9709E7044@EXCHMB-02.stowers-institute.org>

What a splendid idea :-) 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson
Sent: Friday, June 17, 2011 11:13 AM
To: Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Retirement

Bill,

I am about the same age as you are and would like the same type of locale. Since there are so many of us in this field due to retire in the next 15-18 years maybe we should consider investing in a Histotech Retirement Community. Specimens could be shipped to us for processing and staining. We could set up microtome stations in the common area and we could get together and talk and cut at the same time and do it in a wonderful climate with nice views and of course....at our own pace which will be relaxed and more of a shuffle that a full out sprint. Anyone have any suggestions for naming such a paradise? 

Ok...I'm just getting old and it is Friday..so hope you appreciate my humor.

Have a good weekend everyone!
>From here in the Midwest, where waterfront property is in abundance 
>along the Mighty MO

Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc@mercyhealth.com


>>> "O'Donnell, Bill"  6/17/2011 11:00 
>>> AM >>>

 OK, I know it is Friday, and I know that this may sound like a bit of a joke....but.... I am 15-18 years out from retirement and my wife and I want to retire someplace tropical.... And it would be smart to get settled in such a location. So, if anyone knows of any openings in Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would prefer a US territory. I can be reached at bill@deaconbill.com 

William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety Officer Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.



 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From tpodawiltz <@t> lrgh.org  Fri Jun 17 11:42:50 2011
From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas)
Date: Fri Jun 17 11:43:42 2011
Subject: [Histonet] Retirement
In-Reply-To: <4DFB36D7.D67D.00AF.1@mercyhealth.com>
References: 
	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>,
	<4DFB36D7.D67D.00AF.1@mercyhealth.com>
Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3A@LRGHEXVS1.practice.lrgh.org>

I 'm game for that as long as there is Tequila around. 



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
 _______________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson [robinsoc@mercyhealth.com]
Sent: Friday, June 17, 2011 12:13 PM
To: Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Retirement

Bill,

I am about the same age as you are and would like the same type of locale. Since there are so many of us in this field due to retire in the next 15-18 years maybe we should consider investing in a Histotech Retirement Community. Specimens could be shipped to us for processing and staining. We could set up microtome stations in the common area and we could get together and talk and cut at the same time and do it in a wonderful climate with nice views and of course....at our own pace which will be relaxed and more of a shuffle that a full out sprint. Anyone have any suggestions for naming such a paradise?

Ok...I'm just getting old and it is Friday..so hope you appreciate my humor.

Have a good weekend everyone!
>From here in the Midwest, where waterfront property is in abundance along the Mighty MO

Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc@mercyhealth.com


>>> "O'Donnell, Bill"  6/17/2011 11:00 AM >>>

 OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC
Senior Histologist/Safety Officer
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.






_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.  If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare.


From lblazek <@t> digestivespecialists.com  Fri Jun 17 11:48:34 2011
From: lblazek <@t> digestivespecialists.com (Blazek, Linda)
Date: Fri Jun 17 11:48:44 2011
Subject: [Histonet] Retirement
In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3A@LRGHEXVS1.practice.lrgh.org>
References: 
	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>,
	<4DFB36D7.D67D.00AF.1@mercyhealth.com>
	<38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3A@LRGHEXVS1.practice.lrgh.org>
Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBF65EA07@IBMB7Exchange.digestivespecialists.com>

Cup holders on the microtomes since all of us are of the age where we use to have our coffee cups on top of our 'tomes.

Linda

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas
Sent: Friday, June 17, 2011 12:43 PM
To: Cynthia Robinson; Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

I 'm game for that as long as there is Tequila around. 



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
 _______________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson [robinsoc@mercyhealth.com]
Sent: Friday, June 17, 2011 12:13 PM
To: Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Retirement

Bill,

I am about the same age as you are and would like the same type of locale. Since there are so many of us in this field due to retire in the next 15-18 years maybe we should consider investing in a Histotech Retirement Community. Specimens could be shipped to us for processing and staining. We could set up microtome stations in the common area and we could get together and talk and cut at the same time and do it in a wonderful climate with nice views and of course....at our own pace which will be relaxed and more of a shuffle that a full out sprint. Anyone have any suggestions for naming such a paradise?

Ok...I'm just getting old and it is Friday..so hope you appreciate my humor.

Have a good weekend everyone!
>From here in the Midwest, where waterfront property is in abundance along the Mighty MO

Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc@mercyhealth.com


>>> "O'Donnell, Bill"  6/17/2011 11:00 AM >>>

 OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC
Senior Histologist/Safety Officer
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.






_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.  If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From j.brinker <@t> att.net  Fri Jun 17 11:54:14 2011
From: j.brinker <@t> att.net (Jean Brinker)
Date: Fri Jun 17 11:55:39 2011
Subject: [Histonet] Retirement
In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EBF65EA07@IBMB7Exchange.digestivespecialists.com>
References: <4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>,
	<4DFB36D7.D67D.00AF.1@mercyhealth.com><38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3A@LRGHEXVS1.practice.lrgh.org><5A2BD13465E061429D6455C8D6B40E390EBF65EA07@IBMB7Exchange.digestivespecialists.com>
Message-ID: <1768554268-1308329733-cardhu_decombobulator_blackberry.rim.net-1066509439-@b14.c13.bise6.blackberry>

Count me in. Don't know any of you but feel that we should have met before now!
Sent from my BlackBerry

-----Original Message-----
From: "Blazek, Linda" 
Sender: histonet-bounces@lists.utsouthwestern.edu
Date: Fri, 17 Jun 2011 12:48:34 
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

Cup holders on the microtomes since all of us are of the age where we use to have our coffee cups on top of our 'tomes.

Linda

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas
Sent: Friday, June 17, 2011 12:43 PM
To: Cynthia Robinson; Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

I 'm game for that as long as there is Tequila around. 



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
_______________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson [robinsoc@mercyhealth.com]
Sent: Friday, June 17, 2011 12:13 PM
To: Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Retirement

Bill,

I am about the same age as you are and would like the same type of locale. Since there are so many of us in this field due to retire in the next 15-18 years maybe we should consider investing in a Histotech Retirement Community. Specimens could be shipped to us for processing and staining. We could set up microtome stations in the common area and we could get together and talk and cut at the same time and do it in a wonderful climate with nice views and of course....at our own pace which will be relaxed and more of a shuffle that a full out sprint. Anyone have any suggestions for naming such a paradise?

Ok...I'm just getting old and it is Friday..so hope you appreciate my humor.

Have a good weekend everyone!
>From here in the Midwest, where waterfront property is in abundance along the Mighty MO

Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc@mercyhealth.com


>>> "O'Donnell, Bill"  6/17/2011 11:00 AM >>>

 OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC
Senior Histologist/Safety Officer
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.






_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.  If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From billodonnell <@t> catholichealth.net  Fri Jun 17 12:00:57 2011
From: billodonnell <@t> catholichealth.net (O'Donnell, Bill)
Date: Fri Jun 17 12:01:12 2011
Subject: [Histonet] Retirement
In-Reply-To: <4DFB36D7.D67D.00AF.1@mercyhealth.com>
References: 
	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>
	<4DFB36D7.D67D.00AF.1@mercyhealth.com>
Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395085072@chimsx08.CHI.catholichealth.net>

Ah... It's one thing to work w a bunch of histo techs.... It would be
quite another living in the same building. I fully expect to be a cranky
old Irishman someday (some woould say I got a good start).... And since
a good part of my memory may well be gone (if genetics mean anything at
all).... I might not want to be around really sharp objects oor others
in general (remember, I'll be cranky).

Bill

-----Original Message-----
From: Cynthia Robinson [mailto:robinsoc@mercyhealth.com] 
Sent: Friday, June 17, 2011 11:13 AM
To: O'Donnell, Bill; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Retirement

Bill,

I am about the same age as you are and would like the same type of
locale. Since there are so many of us in this field due to retire in the
next 15-18 years maybe we should consider investing in a Histotech
Retirement Community. Specimens could be shipped to us for processing
and staining. We could set up microtome stations in the common area and
we could get together and talk and cut at the same time and do it in a
wonderful climate with nice views and of course....at our own pace which
will be relaxed and more of a shuffle that a full out sprint. Anyone
have any suggestions for naming such a paradise? 

Ok...I'm just getting old and it is Friday..so hope you appreciate my
humor.

Have a good weekend everyone!
>From here in the Midwest, where waterfront property is in abundance
along the Mighty MO

Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc@mercyhealth.com


>>> "O'Donnell, Bill"  6/17/2011 11:00 
>>> AM >>>

 OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com 

William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety
Officer Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.



 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From mturner <@t> CSILaboratories.com  Fri Jun 17 12:02:02 2011
From: mturner <@t> CSILaboratories.com (Mark Turner)
Date: Fri Jun 17 12:02:47 2011
Subject: ***SUSPECT*** RE: [Histonet] Retirement
In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EBF65EA07@IBMB7Exchange.digestivespecialists.com>
References: <4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>,
	<4DFB36D7.D67D.00AF.1@mercyhealth.com><38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3A@LRGHEXVS1.practice.lrgh.org>
	<5A2BD13465E061429D6455C8D6B40E390EBF65EA07@IBMB7Exchange.digestivespecialists.com>
Message-ID: <28FD5AB4A24C8249BDBED1C7FA7C999A02A9DC19@csi-srv-007.CSI-LABS.local>

Dang, I miss the good old days!  :-)

Mark Turner, HT(ASCP) QIHC


-----Original Message-----
From: Blazek, Linda [mailto:lblazek@digestivespecialists.com] 
Sent: Friday, June 17, 2011 12:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: ***SUSPECT*** RE: [Histonet] Retirement

Cup holders on the microtomes since all of us are of the age where we
use to have our coffee cups on top of our 'tomes.

Linda

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Podawiltz, Thomas
Sent: Friday, June 17, 2011 12:43 PM
To: Cynthia Robinson; Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

I 'm game for that as long as there is Tequila around. 



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
 _______________________________________
From: histonet-bounces@lists.utsouthwestern.edu
[histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia
Robinson [robinsoc@mercyhealth.com]
Sent: Friday, June 17, 2011 12:13 PM
To: Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Retirement

Bill,

I am about the same age as you are and would like the same type of
locale. Since there are so many of us in this field due to retire in the
next 15-18 years maybe we should consider investing in a Histotech
Retirement Community. Specimens could be shipped to us for processing
and staining. We could set up microtome stations in the common area and
we could get together and talk and cut at the same time and do it in a
wonderful climate with nice views and of course....at our own pace which
will be relaxed and more of a shuffle that a full out sprint. Anyone
have any suggestions for naming such a paradise?

Ok...I'm just getting old and it is Friday..so hope you appreciate my
humor.

Have a good weekend everyone!
>From here in the Midwest, where waterfront property is in abundance
along the Mighty MO

Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc@mercyhealth.com


>>> "O'Donnell, Bill"  6/17/2011 11:00
AM >>>

 OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC
Senior Histologist/Safety Officer
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.






_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential
information intended only for the use of the recipient(s) named above.
If you are not the intended recipient, you may not print,distribute, or
copy this message or any attachments.  If you have received this
communication in error, please notify the sender by return e-mail and
delete this message and any attachments from your computer. Any views or
opinions expressed are solely those of the author and do not necessarily
represent those of LRGHealthcare.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From billodonnell <@t> catholichealth.net  Fri Jun 17 12:05:36 2011
From: billodonnell <@t> catholichealth.net (O'Donnell, Bill)
Date: Fri Jun 17 12:05:49 2011
Subject: [Histonet] Retirement
In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3A@LRGHEXVS1.practice.lrgh.org>
References: <4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>,
	<4DFB36D7.D67D.00AF.1@mercyhealth.com>
	<38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3A@LRGHEXVS1.practice.lrgh.org>
Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395085073@chimsx08.CHI.catholichealth.net>

Tom,

I imagine that Tequuila is at a premium in some tropical areas..... But
rum.... 

-----Original Message-----
From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] 
Sent: Friday, June 17, 2011 11:43 AM
To: Cynthia Robinson; O'Donnell, Bill; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

I 'm game for that as long as there is Tequila around. 



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
_______________________________________
From: histonet-bounces@lists.utsouthwestern.edu
[histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia
Robinson [robinsoc@mercyhealth.com]
Sent: Friday, June 17, 2011 12:13 PM
To: Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Retirement

Bill,

I am about the same age as you are and would like the same type of
locale. Since there are so many of us in this field due to retire in the
next 15-18 years maybe we should consider investing in a Histotech
Retirement Community. Specimens could be shipped to us for processing
and staining. We could set up microtome stations in the common area and
we could get together and talk and cut at the same time and do it in a
wonderful climate with nice views and of course....at our own pace which
will be relaxed and more of a shuffle that a full out sprint. Anyone
have any suggestions for naming such a paradise?

Ok...I'm just getting old and it is Friday..so hope you appreciate my
humor.

Have a good weekend everyone!
>From here in the Midwest, where waterfront property is in abundance 
>along the Mighty MO

Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc@mercyhealth.com


>>> "O'Donnell, Bill"  6/17/2011 11:00 
>>> AM >>>

 OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety
Officer Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.






_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential
information intended only for the use of the recipient(s) named above.
If you are not the intended recipient, you may not print,distribute, or
copy this message or any attachments.  If you have received this
communication in error, please notify the sender by return e-mail and
delete this message and any attachments from your computer. Any views or
opinions expressed are solely those of the author and do not necessarily
represent those of LRGHealthcare.



From NMargaryan <@t> childrensmemorial.org  Fri Jun 17 12:07:14 2011
From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira)
Date: Fri Jun 17 12:10:13 2011
Subject: [Histonet] Retirement
In-Reply-To: <4d6674a8-7945-4af6-b036-c6506ae03093@CMHHTCA01.childrensmemorial.org>
References: <4d6674a8-7945-4af6-b036-c6506ae03093@CMHHTCA01.childrensmemorial.org>
Message-ID: 




I am 15-18 years out from retirement also. Count me in as well........

Naira



From plott <@t> uab.edu  Fri Jun 17 12:12:10 2011
From: plott <@t> uab.edu (Patricia F Lott)
Date: Fri Jun 17 12:12:20 2011
Subject: [Histonet] staining of tissue culture plates
Message-ID: 

Dear Gayle:

I have a PI who wants us to do H&E staining on tissue culture plates.  Have you ever done this?  Can you send me a protocol?

Thanks,
Patty Lott
From lblazek <@t> digestivespecialists.com  Fri Jun 17 12:17:27 2011
From: lblazek <@t> digestivespecialists.com (Blazek, Linda)
Date: Fri Jun 17 12:17:30 2011
Subject: [Histonet] Retirement
In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF9395085073@chimsx08.CHI.catholichealth.net>
References: <4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>,
	<4DFB36D7.D67D.00AF.1@mercyhealth.com>
	<38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3A@LRGHEXVS1.practice.lrgh.org>
	<4940DF6D1C5FDF48931B6966AAEF9395085073@chimsx08.CHI.catholichealth.net>
Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBF65EA08@IBMB7Exchange.digestivespecialists.com>

I think we need to send Sally out as a scout since she seems to be the next one to go.

Chilled cup holders on the microtome!  Rum or Tequila!

Bill, I've been around cranky old men all my life.  The rum or tequila should help.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, June 17, 2011 1:06 PM
To: Podawiltz, Thomas; Cynthia Robinson; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

Tom,

I imagine that Tequuila is at a premium in some tropical areas..... But
rum.... 

-----Original Message-----
From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] 
Sent: Friday, June 17, 2011 11:43 AM
To: Cynthia Robinson; O'Donnell, Bill; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

I 'm game for that as long as there is Tequila around. 



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
_______________________________________
From: histonet-bounces@lists.utsouthwestern.edu
[histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia
Robinson [robinsoc@mercyhealth.com]
Sent: Friday, June 17, 2011 12:13 PM
To: Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Retirement

Bill,

I am about the same age as you are and would like the same type of
locale. Since there are so many of us in this field due to retire in the
next 15-18 years maybe we should consider investing in a Histotech
Retirement Community. Specimens could be shipped to us for processing
and staining. We could set up microtome stations in the common area and
we could get together and talk and cut at the same time and do it in a
wonderful climate with nice views and of course....at our own pace which
will be relaxed and more of a shuffle that a full out sprint. Anyone
have any suggestions for naming such a paradise?

Ok...I'm just getting old and it is Friday..so hope you appreciate my
humor.

Have a good weekend everyone!
>From here in the Midwest, where waterfront property is in abundance 
>along the Mighty MO

Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc@mercyhealth.com


>>> "O'Donnell, Bill"  6/17/2011 11:00 
>>> AM >>>

 OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety
Officer Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.






_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential
information intended only for the use of the recipient(s) named above.
If you are not the intended recipient, you may not print,distribute, or
copy this message or any attachments.  If you have received this
communication in error, please notify the sender by return e-mail and
delete this message and any attachments from your computer. Any views or
opinions expressed are solely those of the author and do not necessarily
represent those of LRGHealthcare.



_______________________________________________
Histonet mailing list
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From talulahgosh <@t> gmail.com  Fri Jun 17 12:24:12 2011
From: talulahgosh <@t> gmail.com (Emily Sours)
Date: Fri Jun 17 12:24:16 2011
Subject: [Histonet] Retirement
In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF9395085073@chimsx08.CHI.catholichealth.net>
References: 
	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>
	<4DFB36D7.D67D.00AF.1@mercyhealth.com>
	<38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3A@LRGHEXVS1.practice.lrgh.org>
	<4940DF6D1C5FDF48931B6966AAEF9395085073@chimsx08.CHI.catholichealth.net>
Message-ID: 

Retirement? I think by the time I get to that point, social security will
have run out.
Then again, technology will be so advanced, I can tell stories about the old
days, where I logged on to the bbs by modem to post messages to my friends
and typed in my own html coding.
We didn't have google when I was young!! Our cameras used film! And you
couldn't see how bad your pictures were until you developed that film!!
There was no PCR to sequence your DNA, you ran an agarose gel and hoped for
the best!! You could drink the 100% ethanol, there was no denaturing! (okay
that was before my time) You could smoke in the lab while you sectioned
without gloves!! (okay that was too)

Emily

A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron
From kkmarshall <@t> anthc.org  Fri Jun 17 12:31:07 2011
From: kkmarshall <@t> anthc.org (Marshall, Kimberly K)
Date: Fri Jun 17 12:31:33 2011
Subject: [Histonet] Retirement
In-Reply-To: 
Message-ID: 

Count me in as well...At that age I may be well ready for tropical and
get away from the cold of Alaksa... 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily
Sours
Sent: Friday, June 17, 2011 9:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Retirement

Retirement? I think by the time I get to that point, social security
will have run out.
Then again, technology will be so advanced, I can tell stories about the
old days, where I logged on to the bbs by modem to post messages to my
friends and typed in my own html coding.
We didn't have google when I was young!! Our cameras used film! And you
couldn't see how bad your pictures were until you developed that film!!
There was no PCR to sequence your DNA, you ran an agarose gel and hoped
for the best!! You could drink the 100% ethanol, there was no
denaturing! (okay that was before my time) You could smoke in the lab
while you sectioned without gloves!! (okay that was too)

Emily

A great book should leave you with many experiences, and slightly
exhausted.
You should live several lives while reading it.
-William Styron
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From cpyse <@t> x-celllab.com  Fri Jun 17 12:51:22 2011
From: cpyse <@t> x-celllab.com (Cynthia Pyse)
Date: Fri Jun 17 12:51:40 2011
Subject: [Histonet] Retirement
In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>
References: 
	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>
Message-ID: <002501cc2d17$2bf4c120$83de4360$@com>

As long as we don't have to go back to steel knives, old AO micrtomes and
the autotechnicon I'm in. I am also 15 to 18 years out. What is the world of
histology going to do without us. Who will know how to make a solution of
mucicarmine (not that I do anymore, but I could) or eosin? The tech I train
now look at me like I am speaking a foreign language sometimes. Make me feel
old, but closer to retirement.
Cindy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell,
Bill
Sent: Friday, June 17, 2011 12:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retirement


 OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC 
Senior Histologist/Safety Officer
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.



 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




From sbreeden <@t> nmda.nmsu.edu  Fri Jun 17 13:08:03 2011
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Fri Jun 17 13:08:09 2011
Subject: [Histonet] Retirement
Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF780@nmdamailsvr.nmda.ad.nmsu.edu>

It seems that my reference to RETIREMENT has gotten everyone thinking
about it.  Heh...heh..  It has been suggested that I  reconnoiter in
advance of Those of You Who Won't Be Retiring Before I Do (February 29,
2012, if the creek don't rise...).  I would be happy to perform that
hazardous duty but I need more of those $5.00 donations coming in for
whatever it was that I posted last week (I hope my gray cells will
rejuvenate when I retire).  I won't need the money for travel because I
think I'm right where I need to be.  Have any of you thought about New
Mexico???  Just within the past year, it has occurred to me many times
why this is such a good choice for retirement.  We do not have
hurricanes, we do not have tornados (okay, maybe rarely), we are not
prone to earthquakes, the weather is jolly darn good 90% of the time
(spring is out - way too windy) and we don't have more than a couple or
four inches of snow in the winter.  We don't start our furnace/heater
until November and it's only in use until maybe early April.  The air
conditioner was just put to use two weeks ago and we won't need it past
the first part of September.  Low cost of living, lots of homes
(reasonably priced - info upon request) for sale and the number of
things one can do in New Mexico are practically endless.  We have
everything but a beach (and if California keeps shaking, we might have
beachfront property - not that I'd wish that on California...).  We have
skiing, a big lake (fondly called Elephant Butt [Butte]) for water
activities, stream, river and lake fishing out the kazoo, mountains to
climb, white sands in which to wallow, beautiful sunsets and terrible
drivers.  Oops - that one slipped out!  The margarita (and Bud Light)
are the State Drinks (if one is so inclined) and this is the Land of
Manana (read it like Spanish).  Manana is way much better than "I needed
that right this very minute and no excuses"!  Shaded patios, cool
evening breezes and gorgeous cool summer mornings (at least until 7:00
a.m.).  Besides, I need a replacement beginning March 1, 2012.  Brand
new lab, tech-designed, bright and LEED, tons of space, a separate
storage room for blocks and slides AND a volatile storage room with two
acid cabinets and two xylene/alcohol cabinets and a salary (that's
another subject, I do work for a State...).  Can't have everything, but
this is darned close.

 

And I do not work for the Chamber of Commerce!

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

From billodonnell <@t> catholichealth.net  Fri Jun 17 13:19:47 2011
From: billodonnell <@t> catholichealth.net (O'Donnell, Bill)
Date: Fri Jun 17 13:20:02 2011
Subject: [Histonet] Retirement
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF780@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF780@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395085097@chimsx08.CHI.catholichealth.net>

Well...... Maybe if the whole island paradise thing falls apart, NM
could be an option. I am getting too old to be too cold for too long.
(Nebraska)

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Friday, June 17, 2011 1:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retirement

It seems that my reference to RETIREMENT has gotten everyone thinking
about it.  Heh...heh..  It has been suggested that I  reconnoiter in
advance of Those of You Who Won't Be Retiring Before I Do (February 29,
2012, if the creek don't rise...).  I would be happy to perform that
hazardous duty but I need more of those $5.00 donations coming in for
whatever it was that I posted last week (I hope my gray cells will
rejuvenate when I retire).  I won't need the money for travel because I
think I'm right where I need to be.  Have any of you thought about New
Mexico???  Just within the past year, it has occurred to me many times
why this is such a good choice for retirement.  We do not have
hurricanes, we do not have tornados (okay, maybe rarely), we are not
prone to earthquakes, the weather is jolly darn good 90% of the time
(spring is out - way too windy) and we don't have more than a couple or
four inches of snow in the winter.  We don't start our furnace/heater
until November and it's only in use until maybe early April.  The air
conditioner was just put to use two weeks ago and we won't need it past
the first part of September.  Low cost of living, lots of homes
(reasonably priced - info upon request) for sale and the number of
things one can do in New Mexico are practically endless.  We have
everything but a beach (and if California keeps shaking, we might have
beachfront property - not that I'd wish that on California...).  We have
skiing, a big lake (fondly called Elephant Butt [Butte]) for water
activities, stream, river and lake fishing out the kazoo, mountains to
climb, white sands in which to wallow, beautiful sunsets and terrible
drivers.  Oops - that one slipped out!  The margarita (and Bud Light)
are the State Drinks (if one is so inclined) and this is the Land of
Manana (read it like Spanish).  Manana is way much better than "I needed
that right this very minute and no excuses"!  Shaded patios, cool
evening breezes and gorgeous cool summer mornings (at least until 7:00
a.m.).  Besides, I need a replacement beginning March 1, 2012.  Brand
new lab, tech-designed, bright and LEED, tons of space, a separate
storage room for blocks and slides AND a volatile storage room with two
acid cabinets and two xylene/alcohol cabinets and a salary (that's
another subject, I do work for a State...).  Can't have everything, but
this is darned close.

 

And I do not work for the Chamber of Commerce!

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From b-frederick <@t> northwestern.edu  Fri Jun 17 13:23:15 2011
From: b-frederick <@t> northwestern.edu (Bernice Frederick)
Date: Fri Jun 17 13:23:19 2011
Subject: [Histonet] Retirement
In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3A@LRGHEXVS1.practice.lrgh.org>
References: 	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>,
	<4DFB36D7.D67D.00AF.1@mercyhealth.com>
	<38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3A@LRGHEXVS1.practice.lrgh.org>
Message-ID: <002601cc2d1b$9fb38110$df1a8330$@northwestern.edu>

Pina coladas here.!!!

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz,
Thomas
Sent: Friday, June 17, 2011 11:43 AM
To: Cynthia Robinson; Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

I 'm game for that as long as there is Tequila around. 



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
_______________________________________
From: histonet-bounces@lists.utsouthwestern.edu
[histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson
[robinsoc@mercyhealth.com]
Sent: Friday, June 17, 2011 12:13 PM
To: Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Retirement

Bill,

I am about the same age as you are and would like the same type of locale.
Since there are so many of us in this field due to retire in the next 15-18
years maybe we should consider investing in a Histotech Retirement
Community. Specimens could be shipped to us for processing and staining. We
could set up microtome stations in the common area and we could get together
and talk and cut at the same time and do it in a wonderful climate with nice
views and of course....at our own pace which will be relaxed and more of a
shuffle that a full out sprint. Anyone have any suggestions for naming such
a paradise?

Ok...I'm just getting old and it is Friday..so hope you appreciate my humor.

Have a good weekend everyone!
>From here in the Midwest, where waterfront property is in abundance 
>along the Mighty MO

Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc@mercyhealth.com


>>> "O'Donnell, Bill"  6/17/2011 11:00 
>>> AM >>>

 OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I want
to retire someplace tropical.... And it would be smart to get settled in
such a location. So, if anyone knows of any openings in Hawaii, Virgin
Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety Officer
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.






_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential
information intended only for the use of the recipient(s) named above. If
you are not the intended recipient, you may not print,distribute, or copy
this message or any attachments.  If you have received this communication in
error, please notify the sender by return e-mail and delete this message and
any attachments from your computer. Any views or opinions expressed are
solely those of the author and do not necessarily represent those of
LRGHealthcare.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From smcbride <@t> andrew.cmu.edu  Fri Jun 17 13:27:13 2011
From: smcbride <@t> andrew.cmu.edu (Sean McBride)
Date: Fri Jun 17 13:27:19 2011
Subject: [Histonet] Retirement
In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF780@nmdamailsvr.nmda.ad.nmsu.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF780@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: 

Hey Sally,


I can attest to what you are saying.  :-)  I spent two summers as an undergraduate research scientist at the Inhalation Toxicology Research Institute in Albuquerque, and I had an absolutely wonderful experience.  The rugged countryside is quite beautiful, and truly enchanting.  I would often ride my motorcycle across the various regions of the state, enjoying all the variety that the countryside had to offer.  I have many fond memories of those days, so thanks for sharing.... :-)

~Sean



On Jun 17, 2011, at 2:08 PM, Breeden, Sara wrote:

> It seems that my reference to RETIREMENT has gotten everyone thinking
> about it.  Heh...heh..  It has been suggested that I  reconnoiter in
> advance of Those of You Who Won't Be Retiring Before I Do (February 29,
> 2012, if the creek don't rise...).  I would be happy to perform that
> hazardous duty but I need more of those $5.00 donations coming in for
> whatever it was that I posted last week (I hope my gray cells will
> rejuvenate when I retire).  I won't need the money for travel because I
> think I'm right where I need to be.  Have any of you thought about New
> Mexico???  Just within the past year, it has occurred to me many times
> why this is such a good choice for retirement.  We do not have
> hurricanes, we do not have tornados (okay, maybe rarely), we are not
> prone to earthquakes, the weather is jolly darn good 90% of the time
> (spring is out - way too windy) and we don't have more than a couple or
> four inches of snow in the winter.  We don't start our furnace/heater
> until November and it's only in use until maybe early April.  The air
> conditioner was just put to use two weeks ago and we won't need it past
> the first part of September.  Low cost of living, lots of homes
> (reasonably priced - info upon request) for sale and the number of
> things one can do in New Mexico are practically endless.  We have
> everything but a beach (and if California keeps shaking, we might have
> beachfront property - not that I'd wish that on California...).  We have
> skiing, a big lake (fondly called Elephant Butt [Butte]) for water
> activities, stream, river and lake fishing out the kazoo, mountains to
> climb, white sands in which to wallow, beautiful sunsets and terrible
> drivers.  Oops - that one slipped out!  The margarita (and Bud Light)
> are the State Drinks (if one is so inclined) and this is the Land of
> Manana (read it like Spanish).  Manana is way much better than "I needed
> that right this very minute and no excuses"!  Shaded patios, cool
> evening breezes and gorgeous cool summer mornings (at least until 7:00
> a.m.).  Besides, I need a replacement beginning March 1, 2012.  Brand
> new lab, tech-designed, bright and LEED, tons of space, a separate
> storage room for blocks and slides AND a volatile storage room with two
> acid cabinets and two xylene/alcohol cabinets and a salary (that's
> another subject, I do work for a State...).  Can't have everything, but
> this is darned close.
> 
> 
> 
> And I do not work for the Chamber of Commerce!
> 
> 
> 
> Sally Breeden, HT(ASCP)
> 
> New Mexico Department of Agriculture
> 
> Veterinary Diagnostic Services
> 
> 1101 Camino de Salud NE
> 
> Albuquerque, NM  87102
> 
> 505-383-9278 (Histology Lab)
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From billodonnell <@t> catholichealth.net  Fri Jun 17 13:33:24 2011
From: billodonnell <@t> catholichealth.net (O'Donnell, Bill)
Date: Fri Jun 17 13:33:42 2011
Subject: [Histonet] Retirement
In-Reply-To: <002501cc2d17$2bf4c120$83de4360$@com>
References: 
	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>
	<002501cc2d17$2bf4c120$83de4360$@com>
Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395085099@chimsx08.CHI.catholichealth.net>


 I have recently traveled back from the future, and it does not bode
well for histologists. The robots are pretty reliable and can operate 14
hours on a single charge. They also do not have to wear safety glasses
when working with the laser microtomes. No vacations, no calling in
sick, no travelling to seminars (new software version upgrades come to
them)

I noticed that someone still has to come in in the morning to flip the
switches, but since CLIA will not likely consider that testing.... Well,
who'll need a histologist for that?

Funny though, pathologists are still around, but they are all workiing
in a single building near Area 72. (you don't want to know about area 72
unless you are still around in 2035, then EVERYONE will know about area
72) 

They spend their full 6 hour day looking at scanned images on big-screen
monitors and drinking coffee. It's not that the future really needs
them, but the AMA still lobbies for them more effectively than the ASCP
for us. 

- Bill

With apologies to all the really decent pathologists who monitor this
forum. (I have to be nice because my only usable skill someday might be
making a mean cup of coffee)

-----Original Message-----
From: Cynthia Pyse [mailto:cpyse@x-celllab.com] 
Sent: Friday, June 17, 2011 12:51 PM
To: O'Donnell, Bill; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

As long as we don't have to go back to steel knives, old AO micrtomes
and the autotechnicon I'm in. I am also 15 to 18 years out. What is the
world of histology going to do without us. Who will know how to make a
solution of mucicarmine (not that I do anymore, but I could) or eosin?
The tech I train now look at me like I am speaking a foreign language
sometimes. Make me feel old, but closer to retirement.
Cindy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
O'Donnell, Bill
Sent: Friday, June 17, 2011 12:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retirement


 OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety
Officer Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.



 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





From sgoebel <@t> mirnarx.com  Fri Jun 17 13:45:59 2011
From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com)
Date: Fri Jun 17 13:46:03 2011
Subject: [Histonet] Retirement
In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF9395085099@chimsx08.CHI.catholichealth.net>
References: <4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net><002501cc2d17$2bf4c120$83de4360$@com>
	<4940DF6D1C5FDF48931B6966AAEF9395085099@chimsx08.CHI.catholichealth.net>
Message-ID: 

2035 huh??  Crap!!  I won't be retired by then I'll be 54!  Hmm...guess
I need to invent the histo. robot so I can still have income!! 
=)

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
O'Donnell, Bill
Sent: Friday, June 17, 2011 1:33 PM
To: Cynthia Pyse; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement


 I have recently traveled back from the future, and it does not bode
well for histologists. The robots are pretty reliable and can operate 14
hours on a single charge. They also do not have to wear safety glasses
when working with the laser microtomes. No vacations, no calling in
sick, no travelling to seminars (new software version upgrades come to
them)

I noticed that someone still has to come in in the morning to flip the
switches, but since CLIA will not likely consider that testing.... Well,
who'll need a histologist for that?

Funny though, pathologists are still around, but they are all workiing
in a single building near Area 72. (you don't want to know about area 72
unless you are still around in 2035, then EVERYONE will know about area
72) 

They spend their full 6 hour day looking at scanned images on big-screen
monitors and drinking coffee. It's not that the future really needs
them, but the AMA still lobbies for them more effectively than the ASCP
for us. 

- Bill

With apologies to all the really decent pathologists who monitor this
forum. (I have to be nice because my only usable skill someday might be
making a mean cup of coffee)

-----Original Message-----
From: Cynthia Pyse [mailto:cpyse@x-celllab.com] 
Sent: Friday, June 17, 2011 12:51 PM
To: O'Donnell, Bill; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

As long as we don't have to go back to steel knives, old AO micrtomes
and the autotechnicon I'm in. I am also 15 to 18 years out. What is the
world of histology going to do without us. Who will know how to make a
solution of mucicarmine (not that I do anymore, but I could) or eosin?
The tech I train now look at me like I am speaking a foreign language
sometimes. Make me feel old, but closer to retirement.
Cindy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
O'Donnell, Bill
Sent: Friday, June 17, 2011 12:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retirement


 OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety
Officer Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.



 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From CIngles <@t> uwhealth.org  Fri Jun 17 13:51:19 2011
From: CIngles <@t> uwhealth.org (Ingles Claire )
Date: Fri Jun 17 13:55:51 2011
Subject: [Histonet] Retirement
References: <4D14F0FC9316DD41972D5F03C070908B051DF780@nmdamailsvr.nmda.ad.nmsu.edu>
Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A704@UWHC-MAIL2.uwhis.hosp.wisc.edu>

But what about wildfires? :)  
Otherwise send me an application. I have been to New Mexico (mostly Abiquiu area) a few times and loved it. Not too sure about Alberquerque though. Too many episodes of Cops based there. Taos is nice enough. ;)
Claire

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden, Sara
Sent: Fri 6/17/2011 1:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retirement



It seems that my reference to RETIREMENT has gotten everyone thinking
about it.  Heh...heh..  It has been suggested that I  reconnoiter in
advance of Those of You Who Won't Be Retiring Before I Do (February 29,
2012, if the creek don't rise...).  I would be happy to perform that
hazardous duty but I need more of those $5.00 donations coming in for
whatever it was that I posted last week (I hope my gray cells will
rejuvenate when I retire).  I won't need the money for travel because I
think I'm right where I need to be.  Have any of you thought about New
Mexico???  Just within the past year, it has occurred to me many times
why this is such a good choice for retirement.  We do not have
hurricanes, we do not have tornados (okay, maybe rarely), we are not
prone to earthquakes, the weather is jolly darn good 90% of the time
(spring is out - way too windy) and we don't have more than a couple or
four inches of snow in the winter.  We don't start our furnace/heater
until November and it's only in use until maybe early April.  The air
conditioner was just put to use two weeks ago and we won't need it past
the first part of September.  Low cost of living, lots of homes
(reasonably priced - info upon request) for sale and the number of
things one can do in New Mexico are practically endless.  We have
everything but a beach (and if California keeps shaking, we might have
beachfront property - not that I'd wish that on California...).  We have
skiing, a big lake (fondly called Elephant Butt [Butte]) for water
activities, stream, river and lake fishing out the kazoo, mountains to
climb, white sands in which to wallow, beautiful sunsets and terrible
drivers.  Oops - that one slipped out!  The margarita (and Bud Light)
are the State Drinks (if one is so inclined) and this is the Land of
Manana (read it like Spanish).  Manana is way much better than "I needed
that right this very minute and no excuses"!  Shaded patios, cool
evening breezes and gorgeous cool summer mornings (at least until 7:00
a.m.).  Besides, I need a replacement beginning March 1, 2012.  Brand
new lab, tech-designed, bright and LEED, tons of space, a separate
storage room for blocks and slides AND a volatile storage room with two
acid cabinets and two xylene/alcohol cabinets and a salary (that's
another subject, I do work for a State...).  Can't have everything, but
this is darned close.



And I do not work for the Chamber of Commerce!



Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From billodonnell <@t> catholichealth.net  Fri Jun 17 14:08:26 2011
From: billodonnell <@t> catholichealth.net (O'Donnell, Bill)
Date: Fri Jun 17 14:08:42 2011
Subject: [Histonet] Retirement
In-Reply-To: <064F1ACBAE8A78469AE2E41D533D87E505A704@UWHC-MAIL2.uwhis.hosp.wisc.edu>
References: <4D14F0FC9316DD41972D5F03C070908B051DF780@nmdamailsvr.nmda.ad.nmsu.edu>
	<064F1ACBAE8A78469AE2E41D533D87E505A704@UWHC-MAIL2.uwhis.hosp.wisc.edu>
Message-ID: <4940DF6D1C5FDF48931B6966AAEF93950850B0@chimsx08.CHI.catholichealth.net>

This has been a fun thread.... However, I really would like to hear from
anyone looking to hire in tropical island hospitals/labs. - Bill

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles
Claire 
Sent: Friday, June 17, 2011 1:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

But what about wildfires? :)
Otherwise send me an application. I have been to New Mexico (mostly
Abiquiu area) a few times and loved it. Not too sure about Alberquerque
though. Too many episodes of Cops based there. Taos is nice enough. ;)
Claire

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden,
Sara
Sent: Fri 6/17/2011 1:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retirement



It seems that my reference to RETIREMENT has gotten everyone thinking
about it.  Heh...heh..  It has been suggested that I  reconnoiter in
advance of Those of You Who Won't Be Retiring Before I Do (February 29,
2012, if the creek don't rise...).  I would be happy to perform that
hazardous duty but I need more of those $5.00 donations coming in for
whatever it was that I posted last week (I hope my gray cells will
rejuvenate when I retire).  I won't need the money for travel because I
think I'm right where I need to be.  Have any of you thought about New
Mexico???  Just within the past year, it has occurred to me many times
why this is such a good choice for retirement.  We do not have
hurricanes, we do not have tornados (okay, maybe rarely), we are not
prone to earthquakes, the weather is jolly darn good 90% of the time
(spring is out - way too windy) and we don't have more than a couple or
four inches of snow in the winter.  We don't start our furnace/heater
until November and it's only in use until maybe early April.  The air
conditioner was just put to use two weeks ago and we won't need it past
the first part of September.  Low cost of living, lots of homes
(reasonably priced - info upon request) for sale and the number of
things one can do in New Mexico are practically endless.  We have
everything but a beach (and if California keeps shaking, we might have
beachfront property - not that I'd wish that on California...).  We have
skiing, a big lake (fondly called Elephant Butt [Butte]) for water
activities, stream, river and lake fishing out the kazoo, mountains to
climb, white sands in which to wallow, beautiful sunsets and terrible
drivers.  Oops - that one slipped out!  The margarita (and Bud Light)
are the State Drinks (if one is so inclined) and this is the Land of
Manana (read it like Spanish).  Manana is way much better than "I needed
that right this very minute and no excuses"!  Shaded patios, cool
evening breezes and gorgeous cool summer mornings (at least until 7:00
a.m.).  Besides, I need a replacement beginning March 1, 2012.  Brand
new lab, tech-designed, bright and LEED, tons of space, a separate
storage room for blocks and slides AND a volatile storage room with two
acid cabinets and two xylene/alcohol cabinets and a salary (that's
another subject, I do work for a State...).  Can't have everything, but
this is darned close.



And I do not work for the Chamber of Commerce!



Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From lori.garcia <@t> medtronic.com  Fri Jun 17 15:35:44 2011
From: lori.garcia <@t> medtronic.com (Garcia, Lori, Sr. Scientist)
Date: Fri Jun 17 15:36:05 2011
Subject: [Histonet] Retirement
In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF9395085099@chimsx08.CHI.catholichealth.net>
References: 
	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>
	<002501cc2d17$2bf4c120$83de4360$@com>
	<4940DF6D1C5FDF48931B6966AAEF9395085099@chimsx08.CHI.catholichealth.net>
Message-ID: <5A8A2A45BE610D459A95CD6A9102102A0122E5CA34@STSM1BMSGM04.ent.core.medtronic.com>

 Count me in too! Somebody will have to be around for when the robots shut down or revolt and try to take over the world. 

I'm a midwesterner like Cindi, but have relocated to northern CA and it is paradise out here. We could get some land out in the boonies and start our own histo commune.

Lori

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, June 17, 2011 11:33 AM
To: Cynthia Pyse; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement


 I have recently traveled back from the future, and it does not bode well for histologists. The robots are pretty reliable and can operate 14 hours on a single charge. They also do not have to wear safety glasses when working with the laser microtomes. No vacations, no calling in sick, no travelling to seminars (new software version upgrades come to
them)

I noticed that someone still has to come in in the morning to flip the switches, but since CLIA will not likely consider that testing.... Well, who'll need a histologist for that?

Funny though, pathologists are still around, but they are all workiing in a single building near Area 72. (you don't want to know about area 72 unless you are still around in 2035, then EVERYONE will know about area
72) 

They spend their full 6 hour day looking at scanned images on big-screen monitors and drinking coffee. It's not that the future really needs them, but the AMA still lobbies for them more effectively than the ASCP for us. 

- Bill

With apologies to all the really decent pathologists who monitor this forum. (I have to be nice because my only usable skill someday might be making a mean cup of coffee)

-----Original Message-----
From: Cynthia Pyse [mailto:cpyse@x-celllab.com]
Sent: Friday, June 17, 2011 12:51 PM
To: O'Donnell, Bill; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

As long as we don't have to go back to steel knives, old AO micrtomes and the autotechnicon I'm in. I am also 15 to 18 years out. What is the world of histology going to do without us. Who will know how to make a solution of mucicarmine (not that I do anymore, but I could) or eosin?
The tech I train now look at me like I am speaking a foreign language sometimes. Make me feel old, but closer to retirement.
Cindy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, June 17, 2011 12:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retirement


 OK, I know it is Friday, and I know that this may sound like a bit of a joke....but.... I am 15-18 years out from retirement and my wife and I want to retire someplace tropical.... And it would be smart to get settled in such a location. So, if anyone knows of any openings in Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety Officer Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.



 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[CONFIDENTIALITY AND PRIVACY NOTICE]

Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records.
 
To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com


From tpodawiltz <@t> lrgh.org  Fri Jun 17 16:06:43 2011
From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas)
Date: Fri Jun 17 16:10:40 2011
Subject: [Histonet] Retirement
In-Reply-To: <5A8A2A45BE610D459A95CD6A9102102A0122E5CA34@STSM1BMSGM04.ent.core.medtronic.com>
References: 
	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>
	<002501cc2d17$2bf4c120$83de4360$@com>
	<4940DF6D1C5FDF48931B6966AAEF9395085099@chimsx08.CHI.catholichealth.net>,
	<5A8A2A45BE610D459A95CD6A9102102A0122E5CA34@STSM1BMSGM04.ent.core.medtronic.com>
Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3C@LRGHEXVS1.practice.lrgh.org>

For our own commune, I would look at Bend, Oregon, nothing against northern CA  it is so beautiful, buts its still CA. 
Plus the fact, I was born and raised in Oregon. 

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
 ________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garcia, Lori, Sr. Scientist [lori.garcia@medtronic.com]
Sent: Friday, June 17, 2011 4:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

 Count me in too! Somebody will have to be around for when the robots shut down or revolt and try to take over the world.

I'm a midwesterner like Cindi, but have relocated to northern CA and it is paradise out here. We could get some land out in the boonies and start our own histo commune.

Lori

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, June 17, 2011 11:33 AM
To: Cynthia Pyse; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement


 I have recently traveled back from the future, and it does not bode well for histologists. The robots are pretty reliable and can operate 14 hours on a single charge. They also do not have to wear safety glasses when working with the laser microtomes. No vacations, no calling in sick, no travelling to seminars (new software version upgrades come to
them)

I noticed that someone still has to come in in the morning to flip the switches, but since CLIA will not likely consider that testing.... Well, who'll need a histologist for that?

Funny though, pathologists are still around, but they are all workiing in a single building near Area 72. (you don't want to know about area 72 unless you are still around in 2035, then EVERYONE will know about area
72)

They spend their full 6 hour day looking at scanned images on big-screen monitors and drinking coffee. It's not that the future really needs them, but the AMA still lobbies for them more effectively than the ASCP for us.

- Bill

With apologies to all the really decent pathologists who monitor this forum. (I have to be nice because my only usable skill someday might be making a mean cup of coffee)

-----Original Message-----
From: Cynthia Pyse [mailto:cpyse@x-celllab.com]
Sent: Friday, June 17, 2011 12:51 PM
To: O'Donnell, Bill; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

As long as we don't have to go back to steel knives, old AO micrtomes and the autotechnicon I'm in. I am also 15 to 18 years out. What is the world of histology going to do without us. Who will know how to make a solution of mucicarmine (not that I do anymore, but I could) or eosin?
The tech I train now look at me like I am speaking a foreign language sometimes. Make me feel old, but closer to retirement.
Cindy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, June 17, 2011 12:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retirement


 OK, I know it is Friday, and I know that this may sound like a bit of a joke....but.... I am 15-18 years out from retirement and my wife and I want to retire someplace tropical.... And it would be smart to get settled in such a location. So, if anyone knows of any openings in Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety Officer Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.






_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[CONFIDENTIALITY AND PRIVACY NOTICE]

Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records.

To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.  If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare.


From kstoll <@t> mcw.edu  Fri Jun 17 16:49:12 2011
From: kstoll <@t> mcw.edu (Stoll, Kathryn)
Date: Fri Jun 17 16:49:18 2011
Subject: [Histonet] Looking for IHC tips
Message-ID: <110E7925E2B91945A9B79EDFD0DC2B34E79B257E4F@MCWMBX2.mcwcorp.net>

Does anyone have some tips to work up antibodies on the Dako Autostainer using the FLEX detection kit.  I have run into a few research antibodies that are not staining well or at all.

Thanks

Kathryn Stoll, HT(ASCP)
Depatment of Pathology
Medical College of Wisconsin
9200 W Wisconsin Ave
Milwaukee WI 53226
414.805.1525
kstoll@mcw.edu

From tahseen <@t> brain.net.pk  Fri Jun 17 23:05:29 2011
From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk)
Date: Fri Jun 17 23:05:38 2011
Subject: [Histonet] OCD rasipy required
Message-ID: <58240.203.135.35.66.1308369929.squirrel@brain.net.pk>

Dear All,
We required rasipy of the OCD (Frozen section embadding medum).
We are out of stock.Any one want to shere.
Thanks advanc
Tahseen


From estellamireles <@t> gmail.com  Sat Jun 18 15:02:08 2011
From: estellamireles <@t> gmail.com (Stella Mireles)
Date: Sat Jun 18 15:02:14 2011
Subject: [Histonet] Retirement
In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3C@LRGHEXVS1.practice.lrgh.org>
References: 
	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>
	<002501cc2d17$2bf4c120$83de4360$@com>
	<4940DF6D1C5FDF48931B6966AAEF9395085099@chimsx08.CHI.catholichealth.net>
	<5A8A2A45BE610D459A95CD6A9102102A0122E5CA34@STSM1BMSGM04.ent.core.medtronic.com>
	<38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3C@LRGHEXVS1.practice.lrgh.org>
Message-ID: 

I am 9 yrs from retirement. Earlier once my boys quit going back to school.
B.A. then M.A. now PH.D.
I can scoop the place out for everyone, until you get there. My tan should
be gorgeous by then.

See you there.
Stella

On Fri, Jun 17, 2011 at 4:06 PM, Podawiltz, Thomas wrote:

> For our own commune, I would look at Bend, Oregon, nothing against northern
> CA  it is so beautiful, buts its still CA.
> Plus the fact, I was born and raised in Oregon.
>
> Tom Podawiltz, HT (ASCP)
> Histology Section Head/Laboratory Safety Officer
>  ________________________________________
> From: histonet-bounces@lists.utsouthwestern.edu [
> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garcia, Lori, Sr.
> Scientist [lori.garcia@medtronic.com]
> Sent: Friday, June 17, 2011 4:35 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Retirement
>
>  Count me in too! Somebody will have to be around for when the robots shut
> down or revolt and try to take over the world.
>
> I'm a midwesterner like Cindi, but have relocated to northern CA and it is
> paradise out here. We could get some land out in the boonies and start our
> own histo commune.
>
> Lori
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:
> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
> Sent: Friday, June 17, 2011 11:33 AM
> To: Cynthia Pyse; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Retirement
>
>
>  I have recently traveled back from the future, and it does not bode well
> for histologists. The robots are pretty reliable and can operate 14 hours on
> a single charge. They also do not have to wear safety glasses when working
> with the laser microtomes. No vacations, no calling in sick, no travelling
> to seminars (new software version upgrades come to
> them)
>
> I noticed that someone still has to come in in the morning to flip the
> switches, but since CLIA will not likely consider that testing.... Well,
> who'll need a histologist for that?
>
> Funny though, pathologists are still around, but they are all workiing in a
> single building near Area 72. (you don't want to know about area 72 unless
> you are still around in 2035, then EVERYONE will know about area
> 72)
>
> They spend their full 6 hour day looking at scanned images on big-screen
> monitors and drinking coffee. It's not that the future really needs them,
> but the AMA still lobbies for them more effectively than the ASCP for us.
>
> - Bill
>
> With apologies to all the really decent pathologists who monitor this
> forum. (I have to be nice because my only usable skill someday might be
> making a mean cup of coffee)
>
> -----Original Message-----
> From: Cynthia Pyse [mailto:cpyse@x-celllab.com]
> Sent: Friday, June 17, 2011 12:51 PM
> To: O'Donnell, Bill; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Retirement
>
> As long as we don't have to go back to steel knives, old AO micrtomes and
> the autotechnicon I'm in. I am also 15 to 18 years out. What is the world of
> histology going to do without us. Who will know how to make a solution of
> mucicarmine (not that I do anymore, but I could) or eosin?
> The tech I train now look at me like I am speaking a foreign language
> sometimes. Make me feel old, but closer to retirement.
> Cindy
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell,
> Bill
> Sent: Friday, June 17, 2011 12:01 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Retirement
>
>
>  OK, I know it is Friday, and I know that this may sound like a bit of a
> joke....but.... I am 15-18 years out from retirement and my wife and I want
> to retire someplace tropical.... And it would be smart to get settled in
> such a location. So, if anyone knows of any openings in Hawaii, Virgin
> Islands, St. Thomas, Puerto Rico for an experienced HT
> (ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
> prefer a US territory. I can be reached at bill@deaconbill.com
>
> William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety Officer
> Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847
>
> SERENITY is not freedom from the storm, but peace amid the storm.
>
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> [CONFIDENTIALITY AND PRIVACY NOTICE]
>
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From amosbrooks <@t> gmail.com  Sun Jun 19 11:27:01 2011
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Sun Jun 19 11:27:06 2011
Subject: [Histonet] staining of tissue culture plates
Message-ID: 

Hi,
   This is actually fairly easy. It is already an aqueous environment. Pour
off the buffer and add hematoxylin (time is dependent upon the type of
hematoxylin used, basically the same as staining slides) pour off the
hematoxylin and rinse a couple times with water add acid alcohol briefly
while swirling the plate (10 seconds should suffice) rinse with water add 1%
ammonium hydroxide 30, seconds should be plenty. Rinse in water then 95%
ETOH. Add eosin, 30 seconds should suffice. Rinse in 95% then 100% ETOH. Let
it air dry and coverslip with resinous mounting media if you want to, Some
PIs like to just read directly from the plate. Personally I think the
coverslip looks better, but I would be making it for them not myself. (An
important difference)

Good Luck,
Amos


Message: 5
Date: Fri, 17 Jun 2011 12:12:10 -0500
From: Patricia F Lott 
Subject: [Histonet] staining of tissue culture plates
To: "histonet@lists.utsouthwestern.edu"
       
Message-ID:
       
Content-Type: text/plain; charset="us-ascii"

Dear Gayle:

I have a PI who wants us to do H&E staining on tissue culture plates.  Have
you ever done this?  Can you send me a protocol?

Thanks,
Patty Lott
From amosbrooks <@t> gmail.com  Sun Jun 19 11:31:58 2011
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Sun Jun 19 11:32:04 2011
Subject: [Histonet] Retirement
Message-ID: 

Agarose Gels!
    ... Listen you whipersnapper Agarose is the easy way out. When I learned
it we used to have to make up our own polyacrylamide gels. That was after
having to walk to work up hill both ways in 30 feet of snow!

(No nearer retirement)
Crotchety Amos



Message: 7
Date: Fri, 17 Jun 2011 13:24:12 -0400
From: Emily Sours 
Subject: Re: [Histonet] Retirement
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset=UTF-8

Retirement? I think by the time I get to that point, social security will
have run out.
Then again, technology will be so advanced, I can tell stories about the old
days, where I logged on to the bbs by modem to post messages to my friends
and typed in my own html coding.
We didn't have google when I was young!! Our cameras used film! And you
couldn't see how bad your pictures were until you developed that film!!
There was no PCR to sequence your DNA, you ran an agarose gel and hoped for
the best!! You could drink the 100% ethanol, there was no denaturing! (okay
that was before my time) You could smoke in the lab while you sectioned
without gloves!! (okay that was too)

Emily

A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron
From patpxs <@t> gmail.com  Sun Jun 19 15:19:41 2011
From: patpxs <@t> gmail.com (Paula Sicurello)
Date: Sun Jun 19 15:19:45 2011
Subject: [Histonet] Retirement
In-Reply-To: 
References: 
Message-ID: 

Sheesh!  We used to have people smoke while working with propylene oxide.

Eating in your control pigs was part of the benefit of being a
graduate student to save on grocery money.

Film?  My TEM used glass plates.

Lab mates used to routinely drink diet coke and 100% ethanol on Fridays.

Wearing closed toed shoes was for wimps, you were just fast if you
dropped a steel wedge blade.

We even wrote using the entire word and proper grammar, none of this
acronym stuff for us.

Retirement?  What's that?

Paula :-)

On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks  wrote:
> Agarose Gels!
> ? ?... Listen you whipersnapper Agarose is the easy way out. When I learned
> it we used to have to make up our own polyacrylamide gels. That was after
> having to walk to work up hill both ways in 30 feet of snow!
>
> (No nearer retirement)
> Crotchety Amos
>
>
>
> Message: 7
> Date: Fri, 17 Jun 2011 13:24:12 -0400
> From: Emily Sours 
> Subject: Re: [Histonet] Retirement
> To: histonet@lists.utsouthwestern.edu
> Message-ID: 
> Content-Type: text/plain; charset=UTF-8
>
> Retirement? I think by the time I get to that point, social security will
> have run out.
> Then again, technology will be so advanced, I can tell stories about the old
> days, where I logged on to the bbs by modem to post messages to my friends
> and typed in my own html coding.
> We didn't have google when I was young!! Our cameras used film! And you
> couldn't see how bad your pictures were until you developed that film!!
> There was no PCR to sequence your DNA, you ran an agarose gel and hoped for
> the best!! You could drink the 100% ethanol, there was no denaturing! (okay
> that was before my time) You could smoke in the lab while you sectioned
> without gloves!! (okay that was too)
>
> Emily
>
> A great book should leave you with many experiences, and slightly exhausted.
> You should live several lives while reading it.
> -William Styron
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

From Ramona_Nelson <@t> bd.com  Sun Jun 19 15:20:39 2011
From: Ramona_Nelson <@t> bd.com (Ramona_Nelson@bd.com)
Date: Sun Jun 19 15:20:49 2011
Subject: [Histonet] AUTO: Ramona Nelson is out of the office. (returning
	06/21/2011)
Message-ID: 


   


   I am out of the office until 06/21/20   

   
I    

   

   
Note:  This  is  an  automated  response  to  your message &quo   t;Histonet  Digest,  Vol  91,  Issue  27"   PM.
      
This  is  the  only  notification  you will receive whi   person is away.
   

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From laurie.reilly <@t> jcu.edu.au  Sun Jun 19 18:55:49 2011
From: laurie.reilly <@t> jcu.edu.au (Reilly, Laurie)
Date: Sun Jun 19 18:56:35 2011
Subject: [Histonet] Retirement
In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EBF65EA07@IBMB7Exchange.digestivespecialists.com>
References: 
	<4940DF6D1C5FDF48931B6966AAEF9395085054@chimsx08.CHI.catholichealth.net>,
	<4DFB36D7.D67D.00AF.1@mercyhealth.com>
	<38667E7FB77ECD4E91BFAEB8D986386323DEA0CE3A@LRGHEXVS1.practice.lrgh.org>
	<5A2BD13465E061429D6455C8D6B40E390EBF65EA07@IBMB7Exchange.digestivespecialists.com>
Message-ID: 

Tropical Australia might be a great place for a Histo commune. Please hurry with the organising, I'm out of here in 4-6 years and a very wise social worker once told me that you have to retire 'to' something not 'from' something. 
Here in Townsville we are in the middle of our "winter". Minimum overnight about 10C and during the day we have clear blue sunny skies and maximum of mid 20s. We have to put up with that for another 3 months yet. The disadvantage is that it probably won't rain until Christmas and then we get rain by the bucketful.
But 300 sunny days a year is good.

Sorry, I don't have any jobs to offer. We just employed another Histo scientist, one of my students returned from 7 years in private path. Great. Now I have time to read the Histonet.

Regards,    Laurie.



Mr. Laurie REILLY
Histopathology
School of  Veterinary and Biomedical Sciences
James Cook University
Townsville  Qld.  4811
Australia.

Phone 07 4781 4468

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Saturday, 18 June 2011 2:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

Cup holders on the microtomes since all of us are of the age where we use to have our coffee cups on top of our 'tomes.

Linda

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas
Sent: Friday, June 17, 2011 12:43 PM
To: Cynthia Robinson; Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Retirement

I 'm game for that as long as there is Tequila around. 



Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
 _______________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson [robinsoc@mercyhealth.com]
Sent: Friday, June 17, 2011 12:13 PM
To: Bill O'Donnell; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Retirement

Bill,

I am about the same age as you are and would like the same type of locale. Since there are so many of us in this field due to retire in the next 15-18 years maybe we should consider investing in a Histotech Retirement Community. Specimens could be shipped to us for processing and staining. We could set up microtome stations in the common area and we could get together and talk and cut at the same time and do it in a wonderful climate with nice views and of course....at our own pace which will be relaxed and more of a shuffle that a full out sprint. Anyone have any suggestions for naming such a paradise?

Ok...I'm just getting old and it is Friday..so hope you appreciate my humor.

Have a good weekend everyone!
>From here in the Midwest, where waterfront property is in abundance along the Mighty MO

Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc@mercyhealth.com


>>> "O'Donnell, Bill"  6/17/2011 11:00 AM >>>

 OK, I know it is Friday, and I know that this may sound like a bit of a
joke....but.... I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical.... And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC .... PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at bill@deaconbill.com

William (Bill) O'Donnell, HT (ASCP) QIHC
Senior Histologist/Safety Officer
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.






_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.  If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare.


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From histotech <@t> imagesbyhopper.com  Sun Jun 19 19:47:35 2011
From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com)
Date: Sun Jun 19 19:47:54 2011
Subject: [Histonet] Retirement
In-Reply-To: 
References: 
	
Message-ID: 

And remember mouth pipeting? "oops, that's the cotton..."

Eating and smoking in the lab was the norm.

Our alcohol had the tax stamp on it!  :o)

Michelle


On Jun 19, 2011, at 4:19 PM, Paula Sicurello  wrote:

> Sheesh!  We used to have people smoke while working with propylene oxide.
> 
> Eating in your control pigs was part of the benefit of being a
> graduate student to save on grocery money.
> 
> Film?  My TEM used glass plates.
> 
> Lab mates used to routinely drink diet coke and 100% ethanol on Fridays.
> 
> Wearing closed toed shoes was for wimps, you were just fast if you
> dropped a steel wedge blade.
> 
> We even wrote using the entire word and proper grammar, none of this
> acronym stuff for us.
> 
> Retirement?  What's that?
> 
> Paula :-)
> 
> On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks  wrote:
>> Agarose Gels!
>>    ... Listen you whipersnapper Agarose is the easy way out. When I learned
>> it we used to have to make up our own polyacrylamide gels. That was after
>> having to walk to work up hill both ways in 30 feet of snow!
>> 
>> (No nearer retirement)
>> Crotchety Amos
>> 
>> 
>> 
>> Message: 7
>> Date: Fri, 17 Jun 2011 13:24:12 -0400
>> From: Emily Sours 
>> Subject: Re: [Histonet] Retirement
>> To: histonet@lists.utsouthwestern.edu
>> Message-ID: 
>> Content-Type: text/plain; charset=UTF-8
>> 
>> Retirement? I think by the time I get to that point, social security will
>> have run out.
>> Then again, technology will be so advanced, I can tell stories about the old
>> days, where I logged on to the bbs by modem to post messages to my friends
>> and typed in my own html coding.
>> We didn't have google when I was young!! Our cameras used film! And you
>> couldn't see how bad your pictures were until you developed that film!!
>> There was no PCR to sequence your DNA, you ran an agarose gel and hoped for
>> the best!! You could drink the 100% ethanol, there was no denaturing! (okay
>> that was before my time) You could smoke in the lab while you sectioned
>> without gloves!! (okay that was too)
>> 
>> Emily
>> 
>> A great book should leave you with many experiences, and slightly exhausted.
>> You should live several lives while reading it.
>> -William Styron
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From JWeems <@t> sjha.org  Sun Jun 19 22:38:29 2011
From: JWeems <@t> sjha.org (Weems, Joyce)
Date: Sun Jun 19 22:38:54 2011
Subject: [Histonet] Retirement
In-Reply-To: 
References: 
	
	
Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E1640821140DC0@CHEXCMS10.one.ads.che.org>

And the ashes being flicked into the trash with the xylene soaked gauze...... The good ole days!!


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com
Sent: Sunday, June 19, 2011 20:48
To: Paula Sicurello
Cc: histonet@lists.utsouthwestern.edu; Amos Brooks
Subject: Re: [Histonet] Retirement

And remember mouth pipeting? "oops, that's the cotton..."

Eating and smoking in the lab was the norm.

Our alcohol had the tax stamp on it!  :o)

Michelle


On Jun 19, 2011, at 4:19 PM, Paula Sicurello  wrote:

> Sheesh!  We used to have people smoke while working with propylene oxide.
> 
> Eating in your control pigs was part of the benefit of being a 
> graduate student to save on grocery money.
> 
> Film?  My TEM used glass plates.
> 
> Lab mates used to routinely drink diet coke and 100% ethanol on Fridays.
> 
> Wearing closed toed shoes was for wimps, you were just fast if you 
> dropped a steel wedge blade.
> 
> We even wrote using the entire word and proper grammar, none of this 
> acronym stuff for us.
> 
> Retirement?  What's that?
> 
> Paula :-)
> 
> On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks  wrote:
>> Agarose Gels!
>>    ... Listen you whipersnapper Agarose is the easy way out. When I 
>> learned it we used to have to make up our own polyacrylamide gels. 
>> That was after having to walk to work up hill both ways in 30 feet of snow!
>> 
>> (No nearer retirement)
>> Crotchety Amos
>> 
>> 
>> 
>> Message: 7
>> Date: Fri, 17 Jun 2011 13:24:12 -0400
>> From: Emily Sours 
>> Subject: Re: [Histonet] Retirement
>> To: histonet@lists.utsouthwestern.edu
>> Message-ID: 
>> Content-Type: text/plain; charset=UTF-8
>> 
>> Retirement? I think by the time I get to that point, social security 
>> will have run out.
>> Then again, technology will be so advanced, I can tell stories about 
>> the old days, where I logged on to the bbs by modem to post messages 
>> to my friends and typed in my own html coding.
>> We didn't have google when I was young!! Our cameras used film! And 
>> you couldn't see how bad your pictures were until you developed that film!!
>> There was no PCR to sequence your DNA, you ran an agarose gel and 
>> hoped for the best!! You could drink the 100% ethanol, there was no 
>> denaturing! (okay that was before my time) You could smoke in the lab 
>> while you sectioned without gloves!! (okay that was too)
>> 
>> Emily
>> 
>> A great book should leave you with many experiences, and slightly exhausted.
>> You should live several lives while reading it.
>> -William Styron
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This e-mail, including any attachments is the 
property of Catholic Health East and is intended 
for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
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not the intended recipient, please delete this message, and 
reply to the sender regarding the error in a separate email.


From nicole <@t> dlcjax.com  Mon Jun 20 07:40:55 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Mon Jun 20 07:42:19 2011
Subject: [Histonet] Keeping Histo room floor clean?
In-Reply-To: 
References: 
	<54B2B63A-3054-414A-9D4C-97241B2505E7@imagesbyhopper.com>
	
Message-ID: <3369.208.62.167.196.1308573655.squirrel@webmail.realpages.com>

I have a tool that I got from those odd ball lab supply company that carry
a little of everything. Its long like a broom handle and has a scraper
blade on the bottom with a removable replaceable blade. Cost like 20 bucks
works great then I just sweep it up.

Nicole Tatum, HT ASCP


You can buy this type of thing too if you aren't the McGiver type.  For
> instance...from American Mastertech item # CPW04200E
>
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas  78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> histotech@imagesbyhopper.com
> Sent: Tuesday, June 14, 2011 7:50 PM
> To: JR R
> Cc: 
> Subject: Re: [Histonet] Keeping Histo room floor clean?
>
> We don't keep it off the floor, but do use a wide-bladed putty knife
> attached to a mop handle to scrape the residual wax off the floor. It
> woks quite nicely and doesn't remove the actual floor wax like a razor
> blade scrapper would.
>
> Michelle
>
> Sent from my iPhone
>
> On Jun 14, 2011, at 6:44 PM, JR R  wrote:
>
>>
>> We keep getting a lot of paraffin on the floor of one histo
> room--especially around the microtome and the embedding station.
>>
>> Short of laying down a tarp, what do folks do keep wax off of the
> floor?
>>
>> Thanks,
>>
>> Jerry Ricks
>> Research Scientist
>> University of Washington
>> Department of Pathology
>>
> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



From pruegg <@t> ihctech.net  Mon Jun 20 07:58:50 2011
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Mon Jun 20 07:58:53 2011
Subject: [Histonet] Decal Solutions
In-Reply-To: 
References: 
Message-ID: 

I have used formic acid (5%) for decal for IHC for 20 years, it is slower
than HCL but much better for IHC, if you use a platform shaker to keep it
moving the exchange can be pretty good for bm bx 4-6 hours is what I would
start with.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki
Sent: Monday, June 13, 2011 8:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Decal Solutions

Hi,
Does anyone know of any decal solutions that are compatible with IHC, ISH,
FISH other than EDTA and solutions containing HCL ?  We have heard that some
people use formic acid.  Also, what would be the decalcification times for
tissue such as a bone marrow bx using these other solutions ?  Any help
would be greatly appreciated...

Thanks,
Vicki Gauch
AMCH
Albany, NY



-----------------------------------------
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sole use of the individuals or entities to which it is addressed.
If you are not the intended recipient, please notify the sender by
replying to this email and destroying all copies of the
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From Nacaela.Johnson <@t> USONCOLOGY.COM  Mon Jun 20 08:08:21 2011
From: Nacaela.Johnson <@t> USONCOLOGY.COM (Johnson, Nacaela)
Date: Mon Jun 20 08:10:25 2011
Subject: [Histonet] Decal Solutions
In-Reply-To: 
References: 
Message-ID: <71882EED22A283429E8424513A22922D0CD2AD@txhous1eb015.uson.usoncology.int>

We do 95% bone marrow specimens here.  We have perfected our ISH and IHC
using the Rapid-Cal Immuno from BBC.  Most bone marrows decal in 2.5
hours (8 gauge).  The decal does not diminish the iron stores either.
If you have questions about my procedures feel free to contact me. 


Thanks,
 
Nacaela Johnson, B.S. HTL (ASCP)CM
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  Nacaela.Johnson@USOncology.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gauch,
Vicki
Sent: Monday, June 13, 2011 9:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Decal Solutions

Hi,
Does anyone know of any decal solutions that are compatible with IHC,
ISH, FISH other than EDTA and solutions containing HCL ?  We have heard
that some people use formic acid.  Also, what would be the
decalcification times for tissue such as a bone marrow bx using these
other solutions ?  Any help would be greatly appreciated...

Thanks,
Vicki Gauch
AMCH
Albany, NY



-----------------------------------------
CONFIDENTIALITY NOTICE: This email and any attachments may contain
confidential information that is protected by law and is for the sole
use of the individuals or entities to which it is addressed.
If you are not the intended recipient, please notify the sender by
replying to this email and destroying all copies of the communication
and attachments. Further use, disclosure, copying, distribution of, or
reliance upon the contents of this email and attachments is strictly
prohibited. To contact Albany Medical Center, or for a copy of our
privacy practices, please visit us on the Internet at www.amc.edu.
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The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.
Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. From sgoebel <@t> mirnarx.com Mon Jun 20 09:19:14 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Mon Jun 20 09:19:24 2011 Subject: [Histonet] Retirement In-Reply-To: References: Message-ID: The smoking thing is what I missed...I know I work in cancer research and I shouldn't smoke, but as I tell everyone...by the time I get lung cancer I will have helped to find the cure (wishful thinking and stupid excuse making I know.) Now almost everywhere I work you have to completely leave the property to smoke, and the latest talk is that soon we won't be able to even smoke on a public sidewalk! I'm sure in my lifetime I will see cigarettes become illegal and pot become legal, kinda funny I think =) Happy Monday all!! PS-Is anyone else out there going to the Innovex thing in CA this week?? I need a happy hour/go to Alcatraz tour buddy =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Sunday, June 19, 2011 7:48 PM To: Paula Sicurello Cc: histonet@lists.utsouthwestern.edu; Amos Brooks Subject: Re: [Histonet] Retirement And remember mouth pipeting? "oops, that's the cotton..." Eating and smoking in the lab was the norm. Our alcohol had the tax stamp on it! :o) Michelle On Jun 19, 2011, at 4:19 PM, Paula Sicurello wrote: > Sheesh! We used to have people smoke while working with propylene oxide. > > Eating in your control pigs was part of the benefit of being a > graduate student to save on grocery money. > > Film? My TEM used glass plates. > > Lab mates used to routinely drink diet coke and 100% ethanol on Fridays. > > Wearing closed toed shoes was for wimps, you were just fast if you > dropped a steel wedge blade. > > We even wrote using the entire word and proper grammar, none of this > acronym stuff for us. > > Retirement? What's that? > > Paula :-) > > On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks wrote: >> Agarose Gels! >> ... Listen you whipersnapper Agarose is the easy way out. When I learned >> it we used to have to make up our own polyacrylamide gels. That was after >> having to walk to work up hill both ways in 30 feet of snow! >> >> (No nearer retirement) >> Crotchety Amos >> >> >> >> Message: 7 >> Date: Fri, 17 Jun 2011 13:24:12 -0400 >> From: Emily Sours >> Subject: Re: [Histonet] Retirement >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> Content-Type: text/plain; charset=UTF-8 >> >> Retirement? I think by the time I get to that point, social security will >> have run out. >> Then again, technology will be so advanced, I can tell stories about the old >> days, where I logged on to the bbs by modem to post messages to my friends >> and typed in my own html coding. >> We didn't have google when I was young!! Our cameras used film! And you >> couldn't see how bad your pictures were until you developed that film!! >> There was no PCR to sequence your DNA, you ran an agarose gel and hoped for >> the best!! You could drink the 100% ethanol, there was no denaturing! (okay >> that was before my time) You could smoke in the lab while you sectioned >> without gloves!! (okay that was too) >> >> Emily >> >> A great book should leave you with many experiences, and slightly exhausted. >> You should live several lives while reading it. >> -William Styron >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Mon Jun 20 09:31:01 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Mon Jun 20 09:31:19 2011 Subject: [Histonet] Retirement In-Reply-To: References: Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395085110@chimsx08.CHI.catholichealth.net> You had COTTON in your pipettes? We used hollowed out reeds with a bit of papyrus in one end. No calibration.... But plenty accurate enough for histo in those days. However, there are a few things I do not miss from back in da' day. Hand-stropping a knife for an hour, only to have it nicked by a staple in the next hour. -Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Sunday, June 19, 2011 7:48 PM To: Paula Sicurello Cc: histonet@lists.utsouthwestern.edu; Amos Brooks Subject: Re: [Histonet] Retirement And remember mouth pipeting? "oops, that's the cotton..." Eating and smoking in the lab was the norm. Our alcohol had the tax stamp on it! :o) Michelle On Jun 19, 2011, at 4:19 PM, Paula Sicurello wrote: > Sheesh! We used to have people smoke while working with propylene oxide. > > Eating in your control pigs was part of the benefit of being a > graduate student to save on grocery money. > > Film? My TEM used glass plates. > > Lab mates used to routinely drink diet coke and 100% ethanol on Fridays. > > Wearing closed toed shoes was for wimps, you were just fast if you > dropped a steel wedge blade. > > We even wrote using the entire word and proper grammar, none of this > acronym stuff for us. > > Retirement? What's that? > > Paula :-) > > On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks wrote: >> Agarose Gels! >> ... Listen you whipersnapper Agarose is the easy way out. When I >> learned it we used to have to make up our own polyacrylamide gels. >> That was after having to walk to work up hill both ways in 30 feet of snow! >> >> (No nearer retirement) >> Crotchety Amos >> >> >> >> Message: 7 >> Date: Fri, 17 Jun 2011 13:24:12 -0400 >> From: Emily Sours >> Subject: Re: [Histonet] Retirement >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> Content-Type: text/plain; charset=UTF-8 >> >> Retirement? I think by the time I get to that point, social security >> will have run out. >> Then again, technology will be so advanced, I can tell stories about >> the old days, where I logged on to the bbs by modem to post messages >> to my friends and typed in my own html coding. >> We didn't have google when I was young!! Our cameras used film! And >> you couldn't see how bad your pictures were until you developed that film!! >> There was no PCR to sequence your DNA, you ran an agarose gel and >> hoped for the best!! You could drink the 100% ethanol, there was no >> denaturing! (okay that was before my time) You could smoke in the lab >> while you sectioned without gloves!! (okay that was too) >> >> Emily >> >> A great book should leave you with many experiences, and slightly exhausted. >> You should live several lives while reading it. >> -William Styron >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Mon Jun 20 09:40:48 2011 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Jun 20 09:40:56 2011 Subject: [Histonet] Retirement In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF9395085110@chimsx08.CHI.catholichealth.net> References: <4940DF6D1C5FDF48931B6966AAEF9395085110@chimsx08.CHI.catholichealth.net> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386323DEF67D98@LRGHEXVS1.practice.lrgh.org> And that is why I never let you use my knives. Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, June 20, 2011 10:31 AM To: histotech@imagesbyhopper.com; Paula Sicurello Cc: histonet@lists.utsouthwestern.edu; Amos Brooks Subject: RE: [Histonet] Retirement You had COTTON in your pipettes? We used hollowed out reeds with a bit of papyrus in one end. No calibration.... But plenty accurate enough for histo in those days. However, there are a few things I do not miss from back in da' day. Hand-stropping a knife for an hour, only to have it nicked by a staple in the next hour. -Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Sunday, June 19, 2011 7:48 PM To: Paula Sicurello Cc: histonet@lists.utsouthwestern.edu; Amos Brooks Subject: Re: [Histonet] Retirement And remember mouth pipeting? "oops, that's the cotton..." Eating and smoking in the lab was the norm. Our alcohol had the tax stamp on it! :o) Michelle On Jun 19, 2011, at 4:19 PM, Paula Sicurello wrote: > Sheesh! We used to have people smoke while working with propylene oxide. > > Eating in your control pigs was part of the benefit of being a > graduate student to save on grocery money. > > Film? My TEM used glass plates. > > Lab mates used to routinely drink diet coke and 100% ethanol on Fridays. > > Wearing closed toed shoes was for wimps, you were just fast if you > dropped a steel wedge blade. > > We even wrote using the entire word and proper grammar, none of this > acronym stuff for us. > > Retirement? What's that? > > Paula :-) > > On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks wrote: >> Agarose Gels! >> ... Listen you whipersnapper Agarose is the easy way out. When I >> learned it we used to have to make up our own polyacrylamide gels. >> That was after having to walk to work up hill both ways in 30 feet of snow! >> >> (No nearer retirement) >> Crotchety Amos >> >> >> >> Message: 7 >> Date: Fri, 17 Jun 2011 13:24:12 -0400 >> From: Emily Sours >> Subject: Re: [Histonet] Retirement >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> Content-Type: text/plain; charset=UTF-8 >> >> Retirement? I think by the time I get to that point, social security >> will have run out. >> Then again, technology will be so advanced, I can tell stories about >> the old days, where I logged on to the bbs by modem to post messages >> to my friends and typed in my own html coding. >> We didn't have google when I was young!! Our cameras used film! And >> you couldn't see how bad your pictures were until you developed that film!! >> There was no PCR to sequence your DNA, you ran an agarose gel and >> hoped for the best!! You could drink the 100% ethanol, there was no >> denaturing! (okay that was before my time) You could smoke in the lab >> while you sectioned without gloves!! (okay that was too) >> >> Emily >> >> A great book should leave you with many experiences, and slightly exhausted. >> You should live several lives while reading it. >> -William Styron >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From sgoebel <@t> mirnarx.com Mon Jun 20 10:01:55 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Mon Jun 20 10:01:59 2011 Subject: [Histonet] PAS Message-ID: Quick question...if I am doing PAS (w/o digestion) and not looking for fungus...would kidney be an appropriate control?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From bakevictoria <@t> gmail.com Mon Jun 20 10:32:59 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Mon Jun 20 10:33:05 2011 Subject: [Histonet] PAS In-Reply-To: References: Message-ID: Liver - excellent source for glycogen. I haven't used kidney-see what others say. Vikki On Jun 20, 2011 11:02 AM, wrote: > Quick question...if I am doing PAS (w/o digestion) and not looking for > fungus...would kidney be an appropriate control?? > > Thanks > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jun 20 10:52:17 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 20 10:52:21 2011 Subject: [Histonet] PAS In-Reply-To: References: Message-ID: <803739.69800.qm@web65705.mail.ac4.yahoo.com> Yes Ren? J. From: "sgoebel@mirnarx.com" To: histonet@lists.utsouthwestern.edu Sent: Monday, June 20, 2011 11:01 AM Subject: [Histonet] PAS Quick question...if I am doing PAS (w/o digestion) and not looking for fungus...would kidney be an appropriate control?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon Jun 20 11:22:23 2011 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Jun 20 11:23:08 2011 Subject: [Histonet] PAS In-Reply-To: Message-ID: Kidney is a sensitive control for PAS positive material. The basement membranes are PAS positive. It is not positive for glycogen. Sent by: histonet-bounces@lists.utsouthwestern.edu 06/20/2011 08:03 AM To cc Subject [Histonet] PAS Quick question...if I am doing PAS (w/o digestion) and not looking for fungus...would kidney be an appropriate control?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arme <@t> optonline.net Mon Jun 20 11:43:57 2011 From: arme <@t> optonline.net (American Resource Medical) Date: Mon Jun 20 11:44:10 2011 Subject: [Histonet] Mopec MB100 Grossing Station Message-ID: <003d01cc2f69$4026add0$c0740970$@net> This is a used piece in excellent condition and half the price of new. http://media.mopec.com/media/pdf/Catalog2007smaller159.pdf This is a countertop unit. Mark Sofferman, President American ReSource Medical 324 West Englewood Avenue Teaneck, NJ 07666 P: 201.833.1550 F: 201.833.1575 From Lynne.Bell <@t> cvmc.org Mon Jun 20 12:28:39 2011 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Mon Jun 20 12:28:42 2011 Subject: [Histonet] Ventana's HER2 Dual ISH DNA Probe Cocktail Message-ID: Ventana just received FDA approval for the first fully automated diagnostic assay for HER2 gene status determination in breast cancer patients. I do not have a Ventana immunostainer and from what I have been told, it is a closed system and this new probe cocktail could not be used on another stainer, such as the Dako. Is anyone out there in HistoLand using this new cocktail and am I correct that it would not work using the Dako Autostainer. A Ventana rep may contact me with this information also. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center Barre, Vermont 05641 802-371-4923 From wbenton <@t> cua.md Mon Jun 20 12:37:28 2011 From: wbenton <@t> cua.md (Walter Benton) Date: Mon Jun 20 12:40:50 2011 Subject: [Histonet] RE: Ventana's HER2 Dual ISH DNA Probe Cocktail In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD91D6EC7CF76@CUAEXH1.GCU-MD.local> In the past the FDA approval has been tied to running the antibody on the specific vendors' instrumentation using a predetermined protocol. This was true for Dako's EGFR that was FDA approved. Ventana antibodies can be dispensed out of their vials and used on any instrumentation, however, you may have an IVD antibody at that point. Definitely check around and see what others have to say. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne [Lynne.Bell@cvmc.org] Sent: Monday, June 20, 2011 1:28 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Ventana's HER2 Dual ISH DNA Probe Cocktail Ventana just received FDA approval for the first fully automated diagnostic assay for HER2 gene status determination in breast cancer patients. I do not have a Ventana immunostainer and from what I have been told, it is a closed system and this new probe cocktail could not be used on another stainer, such as the Dako. Is anyone out there in HistoLand using this new cocktail and am I correct that it would not work using the Dako Autostainer. A Ventana rep may contact me with this information also. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center Barre, Vermont 05641 802-371-4923 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From Ronald.Houston <@t> nationwidechildrens.org Mon Jun 20 12:48:27 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Jun 20 12:48:39 2011 Subject: [Histonet] RE: Ventana's HER2 Dual ISH DNA Probe Cocktail In-Reply-To: <0B8979A204680A42B93A52B486088CD91D6EC7CF76@CUAEXH1.GCU-MD.local> References: <0B8979A204680A42B93A52B486088CD91D6EC7CF76@CUAEXH1.GCU-MD.local> Message-ID: If you modify any part of the test protocol from the FDA approved methodology in any way, then it is no longer an FDA approved test. Does that mean you cannot use it on your system? Absolutely not; you would have to validate it as you would any other antibody. However, you do need to be cognisant of the fact that if the FDA approved test is dependent on a patient going on a clinical trial or FDA approved treatment for a particular disease, your patient may not be accepted for that treatment regimen. Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Monday, June 20, 2011 1:37 PM To: Bell, Lynne; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Ventana's HER2 Dual ISH DNA Probe Cocktail In the past the FDA approval has been tied to running the antibody on the specific vendors' instrumentation using a predetermined protocol. This was true for Dako's EGFR that was FDA approved. Ventana antibodies can be dispensed out of their vials and used on any instrumentation, however, you may have an IVD antibody at that point. Definitely check around and see what others have to say. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne [Lynne.Bell@cvmc.org] Sent: Monday, June 20, 2011 1:28 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Ventana's HER2 Dual ISH DNA Probe Cocktail Ventana just received FDA approval for the first fully automated diagnostic assay for HER2 gene status determination in breast cancer patients. I do not have a Ventana immunostainer and from what I have been told, it is a closed system and this new probe cocktail could not be used on another stainer, such as the Dako. Is anyone out there in HistoLand using this new cocktail and am I correct that it would not work using the Dako Autostainer. A Ventana rep may contact me with this information also. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center Barre, Vermont 05641 802-371-4923 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. 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From brian <@t> prometheushealthcare.com Mon Jun 20 13:07:00 2011 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Mon Jun 20 13:07:12 2011 Subject: [Histonet] Histotech Openings in Atlanta Message-ID: <002b01cc2f74$dd8deec0$98a9cc40$@com> A dynamic private pathology lab in the Atlanta metro area has several openings for full time qualified ASCP-certified histotechs in routine histology processes including grossing, embedding, cutting, special stains and immunohistochemistry. these are full time openings. at least 3 years experience preferred Please contact me today for immediate consideration Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From Marilyn.A.Weiss <@t> kp.org Mon Jun 20 18:03:08 2011 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Mon Jun 20 18:03:44 2011 Subject: [Histonet] out of office Message-ID: I will be out of the office starting 06/20/2011 and will not return until 06/23/2011. In my absence please ask for Laurie . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. From AnthonyH <@t> chw.edu.au Mon Jun 20 18:20:46 2011 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jun 20 18:20:57 2011 Subject: [Histonet] RE: PAS In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71571887B5CC@xmdb02.nch.kids> Mirna, Lee Luna (Histologic 6(1):77, 1976) suggests that the best control for PAS staining, are the walls of blood vessels. They will show a bright red reaction only if the technique is working well. Glycogen may stain well even with sub-optimal reagents. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Tuesday, 21 June 2011 1:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS Quick question...if I am doing PAS (w/o digestion) and not looking for fungus...would kidney be an appropriate control?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From amitapandey <@t> torrentpharma.com Mon Jun 20 23:34:48 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Mon Jun 20 23:35:47 2011 Subject: [Histonet] PAS In-Reply-To: References: Message-ID: No issue, it can be use. Basement of renal tubules & blood vessel and mesangial matrix of glomeruli gives good result in PAS. Amita From: Victoria Baker To: sgoebel@mirnarx.com Cc: histonet@lists.utsouthwestern.edu Date: 20/06/11 09:08 PM Subject: Re: [Histonet] PAS Sent by: histonet-bounces@lists.utsouthwestern.edu Liver - excellent source for glycogen. I haven't used kidney-see what others say. Vikki On Jun 20, 2011 11:02 AM, wrote: > Quick question...if I am doing PAS (w/o digestion) and not looking for > fungus...would kidney be an appropriate control?? > > Thanks > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kristopher.Kalleberg <@t> unilever.com Tue Jun 21 07:43:41 2011 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Tue Jun 21 07:43:47 2011 Subject: [Histonet] sectioning frozen skin Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C0B1B8942@NTRSEVS30002.s3.ms.unilever.com> Can anyone suggest what the optimal thickness of flash frozen skin sections should be cut at? I typically cut FFPE sections at 5 um, should frozens be cut thinner or thicker? These sections will be used for H&E and routine IHC. Thank you in advance. Kris From Marcia_Gaiser <@t> ssmhc.com Tue Jun 21 08:08:11 2011 From: Marcia_Gaiser <@t> ssmhc.com (Gaiser, Marcia) Date: Tue Jun 21 08:08:17 2011 Subject: [Histonet] Full Time HT Position in Oklahoma City OK Message-ID: <728F817C02110E498D803A7C3B0C6248068D43B58D@S009-APEXM06.ds.ad.ssmhc.com> POSITION REQUIREMENTS: EDUCATION: High school diploma or GED preferred. CERTIFICATION, LICENSURE, BONDING: Certified as an HT or HLT by the American Society of Clinical Pathologists (ASCP) ? or ? other nationally recognized certifying agency acceptable to the Laboratory Director ? or ? experience acceptable to the Laboratory Director. EXPERIENCE: Two years of satisfactory histology experience. SIGN ON BONUS AVAILABLE FOR QUALIFIED CANDIDATES. Outstanding benefits package including generous paid time off. Apply online at www.saintsok.com, Ad# 10762, or contact Anna King at (405) 272-6105 for more information. Thank you! Anna King HR Recruiter St. Anthony Hospital (405) 272-6105 - phone (405) 272-6781 - fax [https://mobile.ssmhc.com/owa/attachment.ashx?id=RgAAAABMsXdcX9YUQLcxZSN9Shu5BwByj4F8AhEOSY2AOnw7DGJIAAUpEpPHAAByj4F8AhEOSY2AOnw7DGJIAAaNIGfxAAAJ&attcnt=1&attid0=EACRQxAfu%2f2MRrpel0TNLrK5] Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From JWeems <@t> sjha.org Tue Jun 21 08:58:58 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Jun 21 08:59:05 2011 Subject: [Histonet] Reference lab reps on site Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E16408210EAE1F@CHEXCMS10.one.ads.che.org> A mini survey... How many of you/your hospitals stopped the reference lab reps from being on site and retrieving tissue from your OR/lab for testing? Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Barry.R.Rittman <@t> uth.tmc.edu Tue Jun 21 09:23:31 2011 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Jun 21 09:23:42 2011 Subject: [Histonet] RE: sectioning frozen skin In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C0B1B8942@NTRSEVS30002.s3.ms.unilever.com> References: <0E6BC087F70F9C47ACFF2C203D6E329C0B1B8942@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E9018D9988A7@UTHCMS1.uthouston.edu> Kris There is no simple answer to this as it depends..... Thinner sections such as 4-5 microns offer better resolution with better localization of many substances within cells. Thicker sections in the 7 to 10 micron range give better perspective of tissue and cell relationship and have more entire cells than the thinner sections. If you are doing any sort of image analysis such as counting numbers, I think that you will find that the thinner you cut sections the greater the variability between sections due to compression, changes in cutting speed and probably also phases of the moon. I would recommend that for your purposes you cut several sections at 5 microns, at 7 and at 10 microns and then compare these to find which best suits your needs. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, June 21, 2011 7:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sectioning frozen skin Can anyone suggest what the optimal thickness of flash frozen skin sections should be cut at? I typically cut FFPE sections at 5 um, should frozens be cut thinner or thicker? These sections will be used for H&E and routine IHC. Thank you in advance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Tue Jun 21 09:35:06 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Jun 21 09:35:12 2011 Subject: [Histonet] Histotech Needed For Lab Outside of Chicago-Full Time/Direct Hire Message-ID: MPath Search Partners, a division of Allied Search Partners is currently looking for a Full Time/Permanent Histotechnician or Histotechnologist in a laboratory outside of Chicago, IL. The position is located about 30 miles to the Northwest of Chicago. Position Title: Histotechnologist/Histotechnician Shift: Monday-Friday, Day Shift 7am-4:30pm (flexible to change the hours slightly for candidate?s availability) Location & Environment: Private Pathology Laboratory Arlington Heights, IL (Outside of Chicago) Preparing & Processing slides (Paraffin) Requirements: Ability to work with no supervision HT or HTL ASCP CLIA eligible to gross To apply: Please send information to alyssa@alliedsearchpartners.com 1. Resume 2. Expected Salary 3. Hours you are available to work Thank you! -- ** Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From alyssa <@t> alliedsearchpartners.com Tue Jun 21 09:37:57 2011 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Jun 21 09:38:01 2011 Subject: [Histonet] Histology Supervisor or Histotech Lead (III) Needed in Fort Myers, FL-Full Time/Day Shift Message-ID: *Position Title: *Histology Supervisor or Histotech Lead (Level III) position *Reports To: *Laboratory Director *Shift: *Monday-Friday, 8am-5pm (or flexible depending on applicants availability) * * *Location:* Pathology Laboratory for well established organization in Fort Myers, FL. * * *Requirements:* * * - ASCP certification required** - Experience leading a team of 2-3 laboratory staff memebers** - Experience and knowledge of OSHA, CLIA, AHCA regulations** - The ability to implement and maintain QA/QC policies and procedures** - Oversee safety and training** - Understanding of the different lab licensing and ranges of testing** * * *Summary:* * * - Responsible for 2-3 other employees within the laboratory** - Work with the Director of Compliance on compliancy of the lab** - Handles all laboratory compliance** - Occasionally help with day to day routine histology* * * * *Benefits:* Health Insurance, Dental Insurance, Vision Insurance. Group Life, Short Term Disability and Long Term Disability paid by employer. Voluntary Life for self, spouse, and child. Discount on employer products and services. PTO for vacation, personal time, sick etc. Paid Holidays (7): New Years Day, Memorial Day, Fourth of July, Labor Day, Thanksgiving Day, Day after Thanksgiving Day, and Christmas Day. Bereavement Leave.* *Bi-annual bonuses, performance reviews, uniform and name badge. Direct Depost & 401K with employer contribution. *To apply:* * * Please send resume and salary expectations to Alyssa@alliedsearchpartners.com . At that time we will contact you to conduct a phone screen. Thank you! -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with ?Remove.? * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From rjbuesa <@t> yahoo.com Tue Jun 21 09:38:21 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 21 09:38:23 2011 Subject: [Histonet] sectioning frozen skin In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C0B1B8942@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <414877.27827.qm@web65702.mail.ac4.yahoo.com> If you are able to cut them at 5 ?m also, that will be fine. Ren? J. --- On Tue, 6/21/11, Kalleberg, Kristopher wrote: From: Kalleberg, Kristopher Subject: [Histonet] sectioning frozen skin To: histonet@lists.utsouthwestern.edu Date: Tuesday, June 21, 2011, 8:43 AM Can anyone suggest what the optimal thickness of flash frozen skin sections should be cut at?? I typically cut FFPE sections at 5 um, should frozens be cut thinner or thicker?? These sections will be used for H&E and routine IHC.? Thank you in advance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Tue Jun 21 10:20:19 2011 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Jun 21 10:20:06 2011 Subject: [Histonet] PT Histotech opening in MS In-Reply-To: References: Message-ID: <03C921A1EAF7F541B16543F6EC6A4B37043AC9A3@giamail2.Gia.com> A GI lab in Ocean Springs, MS is looking for a part-time histotech to work Fri night/Sat. If interested call 601-624-8592 From chak_bou <@t> yahoo.com Tue Jun 21 10:41:58 2011 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Tue Jun 21 10:42:01 2011 Subject: [Histonet] Coronavirus nucleocapsid(CCV2-2) Message-ID: <516278.56687.qm@web161803.mail.bf1.yahoo.com> Hello Histonetters, Is anyone using Coronavirus nucleocapsid(CCV2-2)? If so, can you share with me the staining protocols? can you tell me where you got the secondary antibody? Any input will be very much appreciated. Thank you. Cahkib HTL(ASCP) MIT-DCM From pdunlop720 <@t> gmail.com Tue Jun 21 11:52:17 2011 From: pdunlop720 <@t> gmail.com (Patty Dunlop) Date: Tue Jun 21 11:52:24 2011 Subject: [Histonet] Job Opening for Histotech/Pathology Lab Manager Message-ID: Hello, I am re-posting this opening from May. If you have any questions about the position, please respond to me. Send any application materials to the email listed at the end. Thanks! Patty * * *Histotech Opening in Monterey, CA* *Position Title*: Histotech/Pathology Laboratory Manager *Location*: Physicians Office (GI) Pathology Laboratory in Monterey, CA *Shift*: Full Time, M ? F. Hours are somewhat flexible and must coordinate with pathologists? schedules. *Job Details:* We are looking for an experienced Histology Tech, who will be accountable for operation of our single-tech histology laboratory, and maintain high quality and cost-effective services. This position requires someone who is reliable, hard working, able to work independently and exhibits a strong attention to detail. The Histology tech will perform all duties related to the preparation of surgical GI specimens to be examined by pathologists. This includes but is not limited to:** ? Tissue Processing (microwave) ? Embedding ? Microtomy ? H&E (automated) and Special Staining (manual) ? Cover-slipping (manual) Also performs all duties related to laboratory management, including equipment care and maintenance, inventory/ordering, archiving of specimens, recordkeeping, etc. *We are a formalin-free and xylene-free laboratory. *Job Requirements:* Ability to work with no supervision HT or HTL (ASCP) certification required At least 3 years experience working in histology with little or no supervision Available to start in June 2011 *Compensation: * Competitive salary, health benefits and 401(k) *To apply*: *Please submit resume t*o Helayne Williams at hwilliams@montereygi.com From Jennifer.Bull <@t> northwestpathology.com Tue Jun 21 12:08:36 2011 From: Jennifer.Bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Tue Jun 21 12:08:40 2011 Subject: [Histonet] Looking for Fungus Control Tissue Message-ID: <85760CECEC18444BB95F26D5E88DAEAA22D5FBE58F@hinet2.hinet.org> I am looking for Fungus Control Tissue. Preferably Aspergillus. I have other great control tissues that I can trade if needed. Please contact me at earlybird@cablespeed.com . Thanks!! mailgate.hinet.org made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. --------------------------------------------------------------------- From pruegg <@t> ihctech.net Tue Jun 21 13:51:45 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Jun 21 13:51:51 2011 Subject: [Histonet] sectioning frozen skin In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C0B1B8942@NTRSEVS30002.s3.ms.unilever.com> References: <0E6BC087F70F9C47ACFF2C203D6E329C0B1B8942@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <48BBAFA2ED05419EA9D1FF0F25222C7F@Patsyoffice> I cut frozen sections about the same thickness as ffpe 4-6 microns typically unless thicker is requested, we cut thru embryos and for those they want us to cut 10 micron sections. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, June 21, 2011 6:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sectioning frozen skin Can anyone suggest what the optimal thickness of flash frozen skin sections should be cut at? I typically cut FFPE sections at 5 um, should frozens be cut thinner or thicker? These sections will be used for H&E and routine IHC. Thank you in advance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Tue Jun 21 19:54:18 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Jun 21 19:54:22 2011 Subject: [Histonet] TFE-3 & TFE-B Message-ID: Hi, I have been asked to pass on a question to folks. Is there anyone running TFE-3 and TFE-B antibodies? If so do you take consult cases? I can pass along any answers you may have. I'm sure she will appreciate any help on this matter. Thanks, Amos ----------------------------------------------------------------------------------------------------------- Amos Please ask histoland if a) They have the antibodies and b) Can we send it for consultation? Thanks Mary ----------------------------------------------------------------------------------------------------------- From o.m.gallagher <@t> sheffield.ac.uk Wed Jun 22 06:55:21 2011 From: o.m.gallagher <@t> sheffield.ac.uk (Orla M Gallagher) Date: Wed Jun 22 06:55:30 2011 Subject: [Histonet] EDTA decalcification of bones which have been fixed in 70% ethanol Message-ID: Dear Histonetters, We would like to decalcify some mouse bones in EDTA pH 7 which have been received by our lab already fixed directly in 70% ethanol rather than in formalin or paraformaldehyde. How would you recommend preparing these bones for decalcification e.g. whether to post-fix in formalin or to wash out the ethanol before transferrring to EDTA? I realise that ethanol is not the best fixative to use, especially as the end user may want to do immunocytochemistry or enzyme histochemistry using TRAP. Thanks, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0114-2713337 (office) 0114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk Please think about the environment before printing this email From settembr <@t> umdnj.edu Wed Jun 22 07:31:21 2011 From: settembr <@t> umdnj.edu (Settembre, Dana) Date: Wed Jun 22 07:31:27 2011 Subject: [Histonet] PAX-2 In-Reply-To: <48BBAFA2ED05419EA9D1FF0F25222C7F@Patsyoffice> References: <0E6BC087F70F9C47ACFF2C203D6E329C0B1B8942@NTRSEVS30002.s3.ms.unilever.com> <48BBAFA2ED05419EA9D1FF0F25222C7F@Patsyoffice> Message-ID: Want to purchase Pax-2 for FFPE human tissue. I have been told that there is a countrywide issue that has it on BACKORDER - I have been waiting for about a 3 to 4 weeks. I have been told that a release date may come in a couple of months. I. Does anyone know enough to confirm this? II. Where do you get yours? Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ 07103 USA From paw555 <@t> yahoo.com Wed Jun 22 08:51:26 2011 From: paw555 <@t> yahoo.com (pam plumlee) Date: Wed Jun 22 08:51:30 2011 Subject: [Histonet] Alcohol/Xylene resistant ink stamp Message-ID: <1308750686.44825.YahooMailClassic@web31903.mail.mud.yahoo.com> Histonetters: I'm looking for a hand stamp to use on standard histo glass slides with ink that is alcohol and xylene resistant. The stamp would just need a few lines (company name). Has anyone ever had one of these made? Thanks for any input, Pam Pamela A. Plumlee H.T. (ASCP) bioTheranostics,Inc. pam.plumlee@biotheranostics.com From Audrey.Pagan <@t> nyumc.org Wed Jun 22 09:09:53 2011 From: Audrey.Pagan <@t> nyumc.org (Pagan, Audrey) Date: Wed Jun 22 09:09:59 2011 Subject: [Histonet] Protcol for handling dementia brain biopsies Message-ID: Hello, We are updating our CJD policy and the question of how to handle brain biopsies with a clinical diagnosis of dementia came up. These are biopsies where CJD is not known or suspected. We are considering if we should follow CJD precautions and protocols for these biopsies. I would appreciate any feedback from those of you handling this kind of specimen. Thanks, Audrey Pagan Lab Manager NYU Langone Medical Center New York, NY ------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
================================= 
From FUNKM <@t> mercyhealth.com  Wed Jun 22 09:27:17 2011
From: FUNKM <@t> mercyhealth.com (Marcia Funk)
Date: Wed Jun 22 09:27:25 2011
Subject: [Histonet] Vantage
Message-ID: <4E01B574.E948.00AC.1@mercyhealth.com>

   We are looking at the Vantage system using it with our CoPath system. Please if you
    have some time to chat please give me a call or email.  I would really appreciate
    your help and advice.      
     Thanks Marcia
 
 
Marcia Funk 
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-428-7907
From lblazek <@t> digestivespecialists.com  Wed Jun 22 10:12:55 2011
From: lblazek <@t> digestivespecialists.com (Blazek, Linda)
Date: Wed Jun 22 10:13:00 2011
Subject: [Histonet] H & E stainer
Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBF65EA36@IBMB7Exchange.digestivespecialists.com>

Does anyone out there have a Surgipath Tribune stainer?
Thanks
Linda


Our Vision: To be the #1 choice for all your GI services
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 396-2623
Email: lblazek@digestivespecialists.com

From billodonnell <@t> catholichealth.net  Wed Jun 22 10:47:16 2011
From: billodonnell <@t> catholichealth.net (O'Donnell, Bill)
Date: Wed Jun 22 10:47:34 2011
Subject: [Histonet] Protcol for handling dementia brain biopsies
In-Reply-To: 
References: 
Message-ID: <4940DF6D1C5FDF48931B6966AAEF93950D2D44@chimsx08.CHI.catholichealth.net>

Audrey,

Our policy is to treat brain biopsies for "undiagnosed dementia" as if
it were CJD. 

I don't know if I've been lucky or what, but I have only dealt with one
casein 30 years. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pagan,
Audrey
Sent: Wednesday, June 22, 2011 9:10 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Protcol for handling dementia brain biopsies

Hello,

We are updating our CJD policy and the question of how to handle brain
biopsies with a clinical diagnosis of dementia came up.  These are
biopsies where CJD is not known or suspected.  We are considering if we
should follow CJD precautions and protocols for these biopsies.  I would
appreciate any feedback from those of you handling this kind of
specimen.
Thanks,

Audrey Pagan
Lab Manager
NYU Langone Medical Center
New York, NY
------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
================================= 
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From kblack <@t> digestivehlth.com  Wed Jun 22 11:47:14 2011
From: kblack <@t> digestivehlth.com (Konni Black)
Date: Wed Jun 22 11:47:22 2011
Subject: [Histonet] HT/HTL position Scottsdale, AZ
Message-ID: 

New GI lab in Scottsdale, AZ needs an HT/HTL with experience. Beautiful space with lots of windows! Opening mid-July. Very flexible schedule. Can be part-time or fulltime. Interested parties can contact Konni Black via e-mail kblack@digestivehlth.com or phone 253-503-2560.

Thank you,
Konni  
From rennie1108 <@t> yahoo.com  Wed Jun 22 13:40:46 2011
From: rennie1108 <@t> yahoo.com (Adrienne Anderson)
Date: Wed Jun 22 13:40:50 2011
Subject: [Histonet] Job opening in Columbus, OH
Message-ID: <269137.85887.qm@web59607.mail.ac4.yahoo.com>

Hello Histo-land,

We are a small biotech company seeking a part-time HT or HTL for our laboratory. 
This would be ideal for someone with a full-time job looking for additional work 
hours. As our work load fluctuates, you must be flexible and willing to "go with 
the flow." Experience in embedding, microtomy, and staining is required, but 
most of the work will be focused on microtomy, so a high proficiency in this is 
important. Please send resumes to mollie@phylogenyinc.com

Thanks!
Adrienne
From rjbuesa <@t> yahoo.com  Wed Jun 22 13:54:45 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Jun 22 13:54:49 2011
Subject: [Histonet] EDTA decalcification of bones which have been fixed in
	70% ethanol
In-Reply-To: 
Message-ID: <215999.8850.qm@web65709.mail.ac4.yahoo.com>

Since EDTA is essentially an aqueous solution, I would wash out the 70EthOL, fix in NBF for 24 hours and then place the specimen in EDTA until decalcification is completed.
Ren? J.

--- On Wed, 6/22/11, Orla M Gallagher  wrote:


From: Orla M Gallagher 
Subject: [Histonet] EDTA decalcification of bones which have been fixed in 70% ethanol
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, June 22, 2011, 7:55 AM


Dear Histonetters,

We would like to decalcify some mouse bones in EDTA pH 7 which have been
received by our lab already fixed directly in 70% ethanol rather than in
formalin or paraformaldehyde.

How would you recommend preparing these bones for decalcification e.g.
whether to post-fix in formalin or to wash out the ethanol before
transferrring to EDTA? I realise that ethanol is not the best fixative to
use, especially as the end user may want to do immunocytochemistry or enzyme
histochemistry using TRAP.

Thanks,
Orla

-- 
**************************
Ms. Orla Gallagher
Bone Analysis Laboratory
Mellanby Centre for Bone Research
D Floor Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX

Website: http://mellanbycentre.dept.shef.ac.uk

Tel:? ? ? ???0114-2713337 (office)
? ? ? ? ? ? ? 0114-2713174 (lab)
E-Mail:? ? o.m.gallagher@sheffield.ac.uk

Please think about the environment before printing this email
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From rjbuesa <@t> yahoo.com  Wed Jun 22 13:59:42 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Jun 22 13:59:46 2011
Subject: [Histonet] Protcol for handling dementia brain biopsies
In-Reply-To: 
Message-ID: <302179.16538.qm@web65702.mail.ac4.yahoo.com>

"Dementia" per se, as you wrote, is in no way associated with CJD. It is just a stage/type of Alzheimer's disease in no way caused by a highly contagious prion as in the case of CJD.
IF they are from a known patien with CJD then you have to follow adequate precautions but in the case of behavioural "dementia" in an knwon Alzheimer's patient.
Ren? J.

--- On Wed, 6/22/11, Pagan, Audrey  wrote:


From: Pagan, Audrey 
Subject: [Histonet] Protcol for handling dementia brain biopsies
To: "'histonet@lists.utsouthwestern.edu'" 
Date: Wednesday, June 22, 2011, 10:09 AM


Hello,

We are updating our CJD policy and the question of how to handle brain biopsies with a clinical diagnosis of dementia came up.? These are biopsies where CJD is not known or suspected.? We are considering if we should follow CJD precautions and protocols for these biopsies.? I would appreciate any feedback from those of you handling this kind of specimen.
Thanks,

Audrey Pagan
Lab Manager
NYU Langone Medical Center
New York, NY
------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
================================= 
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From ncosenza <@t> siumed.edu  Wed Jun 22 14:05:57 2011
From: ncosenza <@t> siumed.edu (Nicole Cosenza)
Date: Wed Jun 22 14:06:02 2011
Subject: [Histonet] need a counter stain for esterase and silver stained
	slides
Message-ID: <4E023D15.4090800@siumed.edu>

I have recently stained some muscle tissue with bromoindigo and 
urea-silver to examine the neuromuscular junction.  The nerve axons and 
endplates stain quite well this way, but I'd like to add some color to 
the slide for picture-purposes. Is there a good counterstain (eosin, or 
Biebrich scarlet-acid fuchsin) I can dip the slides in to add color, 
WITHOUT taking away from the stain already present in the slides?

From rjbuesa <@t> yahoo.com  Wed Jun 22 14:12:43 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Jun 22 14:12:46 2011
Subject: [Histonet] need a counter stain for esterase and silver stained
	slides
In-Reply-To: <4E023D15.4090800@siumed.edu>
Message-ID: <65223.49552.qm@web65703.mail.ac4.yahoo.com>

Why don't you try immersing the sections for 1-2 seconds in saturated aqueous picric acid?
Ren? J.

--- On Wed, 6/22/11, Nicole Cosenza  wrote:


From: Nicole Cosenza 
Subject: [Histonet] need a counter stain for esterase and silver stained slides
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, June 22, 2011, 3:05 PM


I have recently stained some muscle tissue with bromoindigo and urea-silver to examine the neuromuscular junction.? The nerve axons and endplates stain quite well this way, but I'd like to add some color to the slide for picture-purposes. Is there a good counterstain (eosin, or Biebrich scarlet-acid fuchsin) I can dip the slides in to add color, WITHOUT taking away from the stain already present in the slides?

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From vjsmee <@t> yahoo.com  Wed Jun 22 15:13:45 2011
From: vjsmee <@t> yahoo.com (smitha rayadurg)
Date: Wed Jun 22 15:13:48 2011
Subject: [Histonet] Formalin Fixation.
Message-ID: <1308773625.15395.YahooMailClassic@web161210.mail.bf1.yahoo.com>

?
?
Dear all
?
Does Formalin fixation of tissues kill bacteria & viruses if present in the tissue?
?
Would you please email any references on the effect of Formalin fixation on the stability of microbes (Viruses, Bacteria Parasites etc) in the tissues?
?
Grateful Thanks
Smitha.
?
?
From rjbuesa <@t> yahoo.com  Wed Jun 22 15:55:52 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Wed Jun 22 15:55:56 2011
Subject: [Histonet] Formalin Fixation.
In-Reply-To: <1308773625.15395.YahooMailClassic@web161210.mail.bf1.yahoo.com>
Message-ID: <552993.11689.qm@web65710.mail.ac4.yahoo.com>

The first uses of formalin by Blum in the late XIX century were to determine its usefulness as a bactericide and, yes, it kills bacteria but not all viruses and no prions.
For references search the web.
Ren? J.

--- On Wed, 6/22/11, smitha rayadurg  wrote:


From: smitha rayadurg 
Subject: [Histonet] Formalin Fixation.
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, June 22, 2011, 4:13 PM


?
?
Dear all
?
Does Formalin fixation of tissues kill bacteria & viruses if present in the tissue?
?
Would you please email any references on the effect of Formalin fixation on the stability of microbes (Viruses, Bacteria Parasites etc) in the tissues?
?
Grateful Thanks
Smitha.
?
?
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From asmith <@t> mail.barry.edu  Wed Jun 22 17:09:59 2011
From: asmith <@t> mail.barry.edu (Smith, Allen)
Date: Wed Jun 22 17:10:08 2011
Subject: [Histonet] Formalin Fixation.
In-Reply-To: <1308773625.15395.YahooMailClassic@web161210.mail.bf1.yahoo.com>
References: <1308773625.15395.YahooMailClassic@web161210.mail.bf1.yahoo.com>
Message-ID: 

Formalin fixation kills most but not all bacteria.  A significant percentage (~10%) of the mycobacteria present in the tissue remain viable.  Tuberculosis bacteria have been cultured from ffpe blocks.  Prions (Creutzfelt-Jacob disease) are believed to survive formalin fixation.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Podiatric Medicine

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of smitha rayadurg
Sent: Wednesday, June 22, 2011 4:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin Fixation.

?
?
Dear all
?
Does Formalin fixation of tissues kill bacteria & viruses if present in the tissue?
?
Would you please email any references on the effect of Formalin fixation on the stability of microbes (Viruses, Bacteria Parasites etc) in the tissues?
?
Grateful Thanks
Smitha.
?
?
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From asmith <@t> mail.barry.edu  Wed Jun 22 17:18:59 2011
From: asmith <@t> mail.barry.edu (Smith, Allen)
Date: Wed Jun 22 17:19:04 2011
Subject: [Histonet] Formalin Fixation.
In-Reply-To: <1308773625.15395.YahooMailClassic@web161210.mail.bf1.yahoo.com>
References: <1308773625.15395.YahooMailClassic@web161210.mail.bf1.yahoo.com>
Message-ID: 

The reference is K.F. Gerston et al: Viability of mycobacteria in formalin-fixed tissues. International Journal of Tuberculosis and Lung Diseases. 2:521-523 (1998).
-Allen A. Smith, Ph.D.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of smitha rayadurg
Sent: Wednesday, June 22, 2011 4:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin Fixation.

?
?
Dear all
?
Does Formalin fixation of tissues kill bacteria & viruses if present in the tissue?
?
Would you please email any references on the effect of Formalin fixation on the stability of microbes (Viruses, Bacteria Parasites etc) in the tissues?
?
Grateful Thanks
Smitha.
?
?
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From MSMACDONALD <@t> PARTNERS.ORG  Thu Jun 23 06:37:38 2011
From: MSMACDONALD <@t> PARTNERS.ORG (Macdonald, Michelle Schwab)
Date: Thu Jun 23 06:37:43 2011
Subject: [Histonet] Ventana ISH
Message-ID: <6C7978C7CEE1CE42B887DD3909B991B30424420B@PHSXMB18.partners.org>


I am looking for others who have switched to the new Ventana ISH probes.  We are
having variable staining and Ventana is unable/unwilling to assist us.  We have
already cleared up the precipitate and are now looking for reproducibility in
the staining.

Any help would be appreciated.

Thanks
Michelle Schwab-MacDonald HT(ASCP), QIHC
Supervisor IHC, Neuropathology, & Special Stains 
Education Coordinator Histopathology
Massachusetts General Hospital
Warren 501D
55 Fruit Street
Boston, MA 02114
617-643-4361
 



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.
From jreichensperger <@t> siumed.edu  Thu Jun 23 09:12:52 2011
From: jreichensperger <@t> siumed.edu (Joel Reichensperger)
Date: Thu Jun 23 09:12:56 2011
Subject: [Histonet] Frozen tissue question
Message-ID: <4E0349E4.3050407@siumed.edu>

We have a new doctor in our lab who swears that all frozen tissue must 
be fixed in formalin with a subsequent sucrose treatment before freezing 
in OCT because not fixing it will cause the structures to be distorted 
and you can't get good antibody attachment. In my previous experience, 
we have done this with tissue that came from an animal that was perfused 
with formalin before the tissue was removed. However, the majority of my 
previous frozen specimens were flash frozen in OCT and fixed after 
sectioning. It is also my understanding that fixation in formalin can 
cause poor antibody detection because of cross-linking caused by the 
formalin. I would like to hear some other opinions on this please. The 
particular specimen we will be working with is skin.

Thanks in advance,
Joel Reichensperger

-- 
Joel Reichensperger
Researcher II
Southern Illinois University
Plastic Surgery Institute
jreichensperger@siumed.edu
217-545-7309 (Office)
217-545-1824 (Fax)


From rjbuesa <@t> yahoo.com  Thu Jun 23 09:38:00 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Jun 23 09:38:04 2011
Subject: [Histonet] Frozen tissue question
In-Reply-To: <4E0349E4.3050407@siumed.edu>
Message-ID: <306256.82765.qm@web65701.mail.ac4.yahoo.com>

Your "new doctor" is absolutely wrong. You do not need to formalin fix or sucrose wash before freezing the specimen in OCT. You can go directly from the fresh specimen?? OCT freezing ? cutting.
You are partially right about the "cross-linking" of the antigens; only "partially" because cross-linking requires about 20 hours and you will never wait so long to make the FS.
In any event, it is not necessary to do what your "new doctor" says; it is evident that s/he is really "new".
Ren? J.
?
?

--- On Thu, 6/23/11, Joel Reichensperger  wrote:


From: Joel Reichensperger 
Subject: [Histonet] Frozen tissue question
To: histonet@lists.utsouthwestern.edu
Date: Thursday, June 23, 2011, 10:12 AM


We have a new doctor in our lab who swears that all frozen tissue must be fixed in formalin with a subsequent sucrose treatment before freezing in OCT because not fixing it will cause the structures to be distorted and you can't get good antibody attachment. In my previous experience, we have done this with tissue that came from an animal that was perfused with formalin before the tissue was removed. However, the majority of my previous frozen specimens were flash frozen in OCT and fixed after sectioning. It is also my understanding that fixation in formalin can cause poor antibody detection because of cross-linking caused by the formalin. I would like to hear some other opinions on this please. The particular specimen we will be working with is skin.

Thanks in advance,
Joel Reichensperger

-- Joel Reichensperger
Researcher II
Southern Illinois University
Plastic Surgery Institute
jreichensperger@siumed.edu
217-545-7309 (Office)
217-545-1824 (Fax)


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From jesus.w.hdz <@t> gmail.com  Thu Jun 23 12:26:21 2011
From: jesus.w.hdz <@t> gmail.com (Jesus Hernandez)
Date: Thu Jun 23 12:26:27 2011
Subject: [Histonet] Removing Paragon/Multistain
Message-ID: 

Dear all, 

I was looking for a way to remove my paragon stain from this magnesium implant that had been embedded in methacrylate. It looks pretty dark and I had decided not to use acetone to try to remove it due to the sample possibly cracking.

Jesse Hernandez ( Grad Student)
University of Texas - San Antonio
Department of Biomedical Engineering
From nicole <@t> dlcjax.com  Thu Jun 23 12:47:50 2011
From: nicole <@t> dlcjax.com (Nicole Tatum)
Date: Thu Jun 23 12:49:47 2011
Subject: [Histonet] Frozen tissue question
In-Reply-To: <4E0349E4.3050407@siumed.edu>
References: <4E0349E4.3050407@siumed.edu>
Message-ID: <1156.208.62.167.196.1308851270.squirrel@webmail.realpages.com>

I run a Mohs lab that processes skin by frozen section. There is no need
to use any fixative before hand. But, if you are looking for melanoma or
melanocytes, freezing can cause artifact and make it difficult to read
slides. Limit the amount of nitrogen you use to freeze the specimen. Some
places use alcohol as a fixative after the slide is cut. It will for all
purposes remove the water continent from the tissue which will preserve
tissue. Hope this helps.

Nicole Tatum HT ASCP





 We have a new doctor in our lab who swears that all frozen tissue must
> be fixed in formalin with a subsequent sucrose treatment before freezing
> in OCT because not fixing it will cause the structures to be distorted
> and you can't get good antibody attachment. In my previous experience,
> we have done this with tissue that came from an animal that was perfused
> with formalin before the tissue was removed. However, the majority of my
> previous frozen specimens were flash frozen in OCT and fixed after
> sectioning. It is also my understanding that fixation in formalin can
> cause poor antibody detection because of cross-linking caused by the
> formalin. I would like to hear some other opinions on this please. The
> particular specimen we will be working with is skin.
>
> Thanks in advance,
> Joel Reichensperger
>
> --
> Joel Reichensperger
> Researcher II
> Southern Illinois University
> Plastic Surgery Institute
> jreichensperger@siumed.edu
> 217-545-7309 (Office)
> 217-545-1824 (Fax)
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



From Larry_Farrand <@t> vwr.com  Thu Jun 23 13:03:37 2011
From: Larry_Farrand <@t> vwr.com (Larry_Farrand@vwr.com)
Date: Thu Jun 23 13:03:44 2011
Subject: [Histonet] Larry Farrand is out of the office
Message-ID: 


I will be out of the office starting  06/22/2011 and will not return until
06/27/2011.

I will not have access to e-mail.  Please contact customer service with any
questions.  If you have an Urgent issue that needs to be addressed, Karen
Longo my regional manager can be contacted to assist you
(karen_longo@vwr.com or 1_847-412-8900)


From amber.mckenzie <@t> gastrodocs.net  Thu Jun 23 15:36:54 2011
From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie)
Date: Thu Jun 23 15:36:29 2011
Subject: [Histonet] AL state meeting?
In-Reply-To: 
References: <03C921A1EAF7F541B16543F6EC6A4B37042B28FD@giamail2.Gia.com>
	
Message-ID: <03C921A1EAF7F541B16543F6EC6A4B37043AD26C@giamail2.Gia.com>


Does anyone know if there's an AL state meeting this year?  

From jesus.w.hdz <@t> gmail.com  Thu Jun 23 15:48:55 2011
From: jesus.w.hdz <@t> gmail.com (Jesus Hernandez)
Date: Thu Jun 23 15:48:59 2011
Subject: [Histonet] Sirius Red Stain
Message-ID: 

Dear all, 

Does anyone have a protocol on how to prepare Sirius Red stain with known concentration. We are unsure about which concentration to use. We are trying to stain collagen. The Sirius Red is in powder-form. Thank you.  

Jesse Hernandez
University of Texas - San Antonio
Department of Biomedical Engineering
From Reuel.Cornelia <@t> tsrh.org  Thu Jun 23 17:00:29 2011
From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia)
Date: Thu Jun 23 17:00:40 2011
Subject: [Histonet] Fwd: Article on the Tornado in the Joplin hospital
References: <4E01B836.0816.00A9.1@tsrh.org> <4E01FDE1.9BC1.007D.3@tsrh.org>
Message-ID: <4E03712D.077E.00C5.1@tsrh.org>

A must read article on code Gray. Very sad story but heroic. 

From CIngles <@t> uwhealth.org  Thu Jun 23 20:01:40 2011
From: CIngles <@t> uwhealth.org (Ingles Claire )
Date: Thu Jun 23 20:06:24 2011
Subject: [Histonet] CE certification
References: <4E01B836.0816.00A9.1@tsrh.org> <4E01FDE1.9BC1.007D.3@tsrh.org>
	<4E03712D.077E.00C5.1@tsrh.org>
Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A70E@UWHC-MAIL2.uwhis.hosp.wisc.edu>

Question to all you ASCP rule gurus - I got my certification before the stipulation involving having so many CE's in order to get recertified every 3 years. In order to be "current" with my certification, do I still need to get recertified every 3 years or am I grandfathered in to the old rules where I don't have to and my certificate is essentially good 'forever'? My boss is trying not to give me my certification bonus because he says I am not current on my certification. Not to be greedy, but $300 is $300.
Claire 
 

 

From lpwenk <@t> sbcglobal.net  Thu Jun 23 21:36:19 2011
From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk)
Date: Thu Jun 23 21:36:23 2011
Subject: [Histonet] CE certification
In-Reply-To: <064F1ACBAE8A78469AE2E41D533D87E505A70E@UWHC-MAIL2.uwhis.hosp.wisc.edu>
References: <4E01B836.0816.00A9.1@tsrh.org>
	<4E01FDE1.9BC1.007D.3@tsrh.org><4E03712D.077E.00C5.1@tsrh.org>
	<064F1ACBAE8A78469AE2E41D533D87E505A70E@UWHC-MAIL2.uwhis.hosp.wisc.edu>
Message-ID: <32374FD36556439798404DF5A0647027@HP2010>

According to the CMP (certification maintenance program) booklet from ASCP 
http://ascp.org/pdf/CMPBooklet.aspx

Page 1:
"The Certification Maintenance Program is required for all individuals who 
became newly certified on or after January 1, 2004 and for all active NCA 
certificants who are not ASCP certified prior to January 1, 2004 in the 
following categories:", and the booklet includes HT and HTL.

Page 2: For everyone else, the booklet states:
"Individuals ASCP certified prior to January 2004 or those who received ASCP 
certification prior to the dates indicated on the chart on page 3, are not 
required to participate in the program; however, voluntary participation is 
encouraged since continuing education provides many personal and 
professional benefits." The dates on "page 3" are the Jan. 1, 2004 for HT 
and HTL mentioned on page 1.

CAP requires continuing education:
GEN.54200 Continuing Education Phase I
There is a functional continuing clinical laboratory education program 
adequate to meet the needs of all personnel.
Evidence of Compliance:
? Written policy for continuing laboratory education
REFERENCES
1) Von Needa P. Keep everyone keen on continuing education. Med Lab Observ. 
1979(May):117-126
2) Berry CD. The JCAH approach to continuing education in the clinical 
laboratory. Lab Med. 1981;12:10-11
3) Umiker W. The role of the pathologist in continuing education programs 
for laboratory personnel. Lab Med. 1981;12:18-21
4) Yapit MK. Resources and strategies for a successful CE program. Med Lab 
Observ. 1989(Apr):47-566
5) Tiehen A. Competency assessment in the transfusion service. Med Lab 
Observ. 1993;25(10):35-42
6) Krienitz DR. Safety education in the laboratory. Lab Med. 1996;27:823-827
7) Nguyen AND, et al. A web-based teaching program for laboratory diagnosis 
of coagulation disorders. Arch Pathol Lab Med. 2000;124:588-593

GEN.54400 Personnel Records Phase II
Technical personnel records include all of the following mandatory items.
1. Summary of training and experience
2. Copy of academic degree or transcript
3. License, if required by state
4. Certification, if required by state or employer
5. Description of current duties and responsibilities as specified by the 
laboratory director: a) Procedures the individual is authorized to perform, 
b) Whether supervision is required for specimen processing, test performance 
or result reporting, c) Whether supervisory or director review is required 
to report patient test results
6. Records of continuing education
7. Records of radiation exposure where applicable (such as with in vivo 
radiation testing), but not required for low exposure levels such as certain 
in-vitro testing
8. Work-related incident and/or accident records
9. Dates of employment
REFERENCES
1) CLSI. Training and Competence Assessment; Approved Guideline?Third 
Edition. CLSI Document GP21-A3. (ISBN 1-56238-691-3).
CLSI, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898 USA, 2009
2) Harmening DM, et al. Defining roles of medical technologists and medical 
laboratory technicians.

Does your place of employment have continuing education requirements? Maybe 
that's what your boss is "quoting". Our lab department has a written CE 
policy that states all full time technicians and technologists need a 
minimum of 12 hours CE each year. (part time tech need 6/year, as do lab 
assistants, office personnel and autopsy assistants.) Less than our required 
CE, our yearly evaluation goes down, more than that and our yearly 
evaluation goes up, in the category of CE/professionalism.

Peggy Wenk
-----Original Message----- 
From: Ingles Claire
Sent: Thursday, June 23, 2011 9:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CE certification

Question to all you ASCP rule gurus - I got my certification before the 
stipulation involving having so many CE's in order to get recertified every 
3 years. In order to be "current" with my certification, do I still need to 
get recertified every 3 years or am I grandfathered in to the old rules 
where I don't have to and my certificate is essentially good 'forever'? My 
boss is trying not to give me my certification bonus because he says I am 
not current on my certification. Not to be greedy, but $300 is $300.
Claire




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From Vivek.Saroha <@t> nottingham.ac.uk  Fri Jun 24 04:05:16 2011
From: Vivek.Saroha <@t> nottingham.ac.uk (Vivek Saroha)
Date: Fri Jun 24 04:14:45 2011
Subject: [Histonet] Macrophage marker in sheep for IHC
Message-ID: <834C946D8CED2340B4A9DC8401B55CE0057D03E826@EXCHANGE1.ad.nottingham.ac.uk>

Hi there,
I am very new to basic sciences research and am planning an experiment to quantify and compare adipose tissue macrophage infiltration in sheep model using immunohistochemistry. I am specifically interested in CD11c as a marker of M1 activation of Macrophages. I have been advised that it is not easy and previous effort by my predecessors have not been very successful in this!
>From the general google, histonet/histosearch and pubmed search. I have made a list of potential candidates:

1.    Clones PG M1 and Clone KP1
(Source : Histonet : http://www.histosearch.com/histonet/Nov03A/RE.HistonetSeepmacrophage.html)


2.    EMB11, a mouse anti-human CD68 mAb (Dako, Carpenteria, CA)
(source: published paper: http://www.ncbi.nlm.nih.gov/pubmed/16460804 )


 1.  Another CD68 antibody
Source: http://www.ingentaconnect.com/content/ocean/ajra/2011/00000025/00000002/art00024)

 1.  Iba1 (ionized calcium binding adaptor molecule 1) is a 17-kDa EF hand protein that is specifically expressed in macrophages/microglia and is upregulated during the activation of these cells.[Wikipedia]
(source: I do not remember where I found this one from but Abcam has several antibodies none of which are predicted for sheep)
I am writing to ask for suggestions about which one to go for and any other tips about searching for antibodies or performing IHC in paraffin fixed adipose tissue/liver.
Any ideas will be gratefully appreciated as I am very new and isolated in this aspect.
Best wishes
Vivek

Dr Vivek Saroha
Clinical lecturer in Child health
University of Nottingham
This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it.   Please do not use, copy or disclose the information contained in this message or in any attachment.  Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham.

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From talulahgosh <@t> gmail.com  Fri Jun 24 04:25:48 2011
From: talulahgosh <@t> gmail.com (Emily Sours)
Date: Fri Jun 24 04:27:45 2011
Subject: [Histonet] Frozen tissue question
In-Reply-To: <1156.208.62.167.196.1308851270.squirrel@webmail.realpages.com>
References: <4E0349E4.3050407@siumed.edu>
	<1156.208.62.167.196.1308851270.squirrel@webmail.realpages.com>
Message-ID: 

>From the research point of view, I've heard of people not fixing tissue
before they section it. Since I work with embryonic tissue (which is mostly
water!), we always fix our tissue. It definitely is better to not fix tissue
when you want to stain with antibodies because anything you do to the sample
might affect the antigens.  However, since it's impossible to section
unfixed tissue, you have to fix it somehow.
Our lab once tried to section snap frozen unfixed chick embryo because
another lab (our Sauron, if you will) had said that's the best way to get
antibody staining.  We, however, could not get the tissue to section
properly.  How that other lab did it is beyond me.
Either way, there's never a clear yes or no with protocols, it'll always
change due to what you're sectioning.

Emily

A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Thu, Jun 23, 2011 at 1:47 PM, Nicole Tatum  wrote:

> I run a Mohs lab that processes skin by frozen section. There is no need
> to use any fixative before hand. But, if you are looking for melanoma or
> melanocytes, freezing can cause artifact and make it difficult to read
> slides. Limit the amount of nitrogen you use to freeze the specimen. Some
> places use alcohol as a fixative after the slide is cut. It will for all
> purposes remove the water continent from the tissue which will preserve
> tissue. Hope this helps.
>
> Nicole Tatum HT ASCP
>
>
>
>
>
>  We have a new doctor in our lab who swears that all frozen tissue must
> > be fixed in formalin with a subsequent sucrose treatment before freezing
> > in OCT because not fixing it will cause the structures to be distorted
> > and you can't get good antibody attachment. In my previous experience,
> > we have done this with tissue that came from an animal that was perfused
> > with formalin before the tissue was removed. However, the majority of my
> > previous frozen specimens were flash frozen in OCT and fixed after
> > sectioning. It is also my understanding that fixation in formalin can
> > cause poor antibody detection because of cross-linking caused by the
> > formalin. I would like to hear some other opinions on this please. The
> > particular specimen we will be working with is skin.
> >
> > Thanks in advance,
> > Joel Reichensperger
> >
> > --
> > Joel Reichensperger
> > Researcher II
> > Southern Illinois University
> > Plastic Surgery Institute
> > jreichensperger@siumed.edu
> > 217-545-7309 (Office)
> > 217-545-1824 (Fax)
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From aonomic <@t> auburn.edu  Fri Jun 24 08:27:33 2011
From: aonomic <@t> auburn.edu (Michelle Aono)
Date: Fri Jun 24 08:27:43 2011
Subject: [Histonet] Tissue Shriveling in Paraffin
Message-ID: <4E044A71.5876.00D9.0@auburn.edu>

I was cutting some bone/joint tissue and noticed that the cartilaginous
portion was concave/indented, instead of flush with the rest of the
block surface.  Even as I continued to cut that portion always seemed a
little sunken into the block face and all the sections crumbled.  I
didn't seal the block after I was done and when I came back the next day
the entire tissue sample was shriveled and pulling away from the
paraffin.  I'm new, but in the few bone sections I've done I've never
had this happen!  Any ideas?

~Shelly

From SAllen <@t> exchange.hsc.mb.ca  Fri Jun 24 08:28:39 2011
From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen)
Date: Fri Jun 24 08:28:44 2011
Subject: [Histonet] SV-40 control blocks or slides
Message-ID: 

Hi,
 I am looking for a lab or a supplier for SV-40 control blocks or
slides. If anyone can help us out it would be appreciated.
Thanks


Sharon Allen
Senior Technologist
Neuropathology Lab MS435U
Health Sciences Centre
825 Sherbrook St.
Winnipeg, Manitoba R3A 1R9
Ph# 787-4615


-------------- next part --------------
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From shive003 <@t> umn.edu  Fri Jun 24 08:37:28 2011
From: shive003 <@t> umn.edu (Jan Shivers)
Date: Fri Jun 24 08:37:31 2011
Subject: [Histonet] Macrophage marker in sheep for IHC
References: <834C946D8CED2340B4A9DC8401B55CE0057D03E826@EXCHANGE1.ad.nottingham.ac.uk>
Message-ID: <4BFB9735C69E4BFFAB66E0ACC0EA1982@auxs.umn.edu>

CD68 - clone KP1 has not worked on sheep (also not dogs, cows, horses, and 
chickens) in my experience.  I have found it to stain primates well (and 
rare cells in pigs and cats).

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003@umn.edu

(Confidentiality Notice: This message, together with any attachments, is 
intended only for the use of the individual or entity to which it is 
addressed and may contain confidential or privileged information. If you 
think you have received this message in error, please advise the sender and 
then delete this message and any attachments immediately.)

----- Original Message ----- 
From: "Vivek Saroha" 
To: 
Sent: Friday, June 24, 2011 4:05 AM
Subject: [Histonet] Macrophage marker in sheep for IHC


Hi there,
I am very new to basic sciences research and am planning an experiment to 
quantify and compare adipose tissue macrophage infiltration in sheep model 
using immunohistochemistry. I am specifically interested in CD11c as a 
marker of M1 activation of Macrophages. I have been advised that it is not 
easy and previous effort by my predecessors have not been very successful in 
this!
>From the general google, histonet/histosearch and pubmed search. I have 
>made a list of potential candidates:

1.    Clones PG M1 and Clone KP1
(Source : Histonet : 
http://www.histosearch.com/histonet/Nov03A/RE.HistonetSeepmacrophage.html)


2.    EMB11, a mouse anti-human CD68 mAb (Dako, Carpenteria, CA)
(source: published paper: http://www.ncbi.nlm.nih.gov/pubmed/16460804 )


 1.  Another CD68 antibody
Source: 
http://www.ingentaconnect.com/content/ocean/ajra/2011/00000025/00000002/art00024)

 1.  Iba1 (ionized calcium binding adaptor molecule 1) is a 17-kDa EF hand 
protein that is specifically expressed in macrophages/microglia and is 
upregulated during the activation of these cells.[Wikipedia]
(source: I do not remember where I found this one from but Abcam has several 
antibodies none of which are predicted for sheep)
I am writing to ask for suggestions about which one to go for and any other 
tips about searching for antibodies or performing IHC in paraffin fixed 
adipose tissue/liver.
Any ideas will be gratefully appreciated as I am very new and isolated in 
this aspect.
Best wishes
Vivek

Dr Vivek Saroha
Clinical lecturer in Child health
University of Nottingham
This message and any attachment are intended solely for the addressee and 
may contain confidential information. If you have received this message in 
error, please send it back to me, and immediately delete it.   Please do not 
use, copy or disclose the information contained in this message or in any 
attachment.  Any views or opinions expressed by the author of this email do 
not necessarily reflect the views of the University of Nottingham.

This message has been checked for viruses but the contents of an attachment
may still contain software viruses which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK 
legislation._______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From ratliffjack <@t> hotmail.com  Fri Jun 24 08:54:47 2011
From: ratliffjack <@t> hotmail.com (Jack Ratliff)
Date: Fri Jun 24 08:54:56 2011
Subject: [Histonet] Tissue Shriveling in Paraffin
In-Reply-To: <4E044A71.5876.00D9.0@auburn.edu>
References: <4E044A71.5876.00D9.0@auburn.edu>
Message-ID: 

Why not try resin embedding techniques? Less shrinkage than paraffin, more specimen/block stability in cutting, no damaging effects of decalcification and a many times you have a better and more clear morphological representation. I could help you to achieve this if interested, so feel free to contact me as needed (31-281-1975).

Jack

Jack Ratliff

Senior Histologist, Biomimetic Therapeutics, Inc.

Chair, Hard Tissue Committee - National Society for Histotechnology



On Jun 24, 2011, at 8:27 AM, "Michelle Aono"  wrote:

> I was cutting some bone/joint tissue and noticed that the cartilaginous
> portion was concave/indented, instead of flush with the rest of the
> block surface.  Even as I continued to cut that portion always seemed a
> little sunken into the block face and all the sections crumbled.  I
> didn't seal the block after I was done and when I came back the next day
> the entire tissue sample was shriveled and pulling away from the
> paraffin.  I'm new, but in the few bone sections I've done I've never
> had this happen!  Any ideas?
> 
> ~Shelly
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From ratliffjack <@t> hotmail.com  Fri Jun 24 08:58:24 2011
From: ratliffjack <@t> hotmail.com (Jack Ratliff)
Date: Fri Jun 24 08:58:36 2011
Subject: [Histonet] Sirius Red Stain
In-Reply-To: 
References: 
Message-ID: 

Why not save yourself some time and simply purchase the staining kit from Polysciences or Dorn and Hart Micredge.

Jack



On Jun 23, 2011, at 3:48 PM, Jesus Hernandez  wrote:

> Dear all, 
> 
> Does anyone have a protocol on how to prepare Sirius Red stain with known concentration. We are unsure about which concentration to use. We are trying to stain collagen. The Sirius Red is in powder-form. Thank you.  
> 
> Jesse Hernandez
> University of Texas - San Antonio
> Department of Biomedical Engineering
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From MSHERWOOD <@t> PARTNERS.ORG  Fri Jun 24 08:58:42 2011
From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret )
Date: Fri Jun 24 08:58:47 2011
Subject: [Histonet] Sirius Red Stain
In-Reply-To: 
References: 
Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9708DB5845@PHSXMB30.partners.org>

We purchase our PicroSirius Red in 0.1% concentration and stain with that.
Works fine. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Hernandez
Sent: Thursday, June 23, 2011 4:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sirius Red Stain

Dear all, 

Does anyone have a protocol on how to prepare Sirius Red stain with known
concentration. We are unsure about which concentration to use. We are trying to
stain collagen. The Sirius Red is in powder-form. Thank you.  

Jesse Hernandez
University of Texas - San Antonio
Department of Biomedical Engineering
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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From Reuel.Cornelia <@t> tsrh.org  Fri Jun 24 09:06:24 2011
From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia)
Date: Fri Jun 24 09:06:39 2011
Subject: [Histonet] Joplin's article
Message-ID: <4E04538F.077E.00C5.1@tsrh.org>

  To those who were not able to open to the site, I tried to copy and
paste and hopefully you can read it. This is a very interesting story
about Joplin's St John's Regional Medical Center and code Gray.
If you still have problems. please visit this link. 
http://m.kansascity.com/kcstar/db_41535/contentdetail.htm?contentguid=0kA2g6z2&src=cat&full=true#display

 
By ERIC ADLER and LAURA BAUER 
The Kansas City Star 
JOPLIN, Mo. | Looking back, they remember the quiet ? like a last, deep
breath before death. 
In the nursery of St. John?s Regional Medical Center, newborns napped
in bassinets. Ventilators hummed in an intensive-care unit. 
In the emergency room, nurse Tracy Hernandez checked an older woman for
a stroke, one of the few serious cases in the ER all day. 
In an operating room down a second-floor hallway, orthopedic surgeon
James ?Dusty? Smith opened an infected hip. 
One floor above, John Seay, a 60-year-old mechanic from Welch, Okla.,
visited with his 83-year-old mother. Frail from congestive heart
failure, she doubted she?d be getting better and had picked out a pink
dress for her burial. 
Then, in the west, the air began to spin. 
Condition Gray. 
The announcement over the hospital speakers warned of a potential
tornado. Prepare. 
No one panicked. Such calls are routine in Joplin, a zinc- and 
lead-mining town carved from the rock and fields of Tornado Alley. 
Nurses pulled shades over windows to shield from flying debris. They
rolled equipment from the halls, on the off chance patients would have
to be moved there. 
Off chance, because this storm wasn?t expected to hit them. Visitors
watched it on television with nonchalance. Radar showed funnel clouds
tracking north. 
In a neighborhood across from St. John?s, Amanda and Bradley German sat
in a friend?s home with their sons, Brody, 6, and James, 9, heedless of
the weather alert. Small hail fell. The friend tossed hailstones
playfully into the house. 
?We were joking about it,? Amanda German would say. ?We hear the storm
sirens all the time.? 
What no one anticipated was the dark monster developing to the west,
two miles outside their windows. 
The sky turned the green of a violent bruise. 
Execute Condition Gray: Get patients to safety! 
? ? ? 
It?s been four weeks since an EF-5 tornado slammed to the ground in
Joplin, its 200-mph winds scouring a three-quarter-mile-wide,
six-mile-long band of devastation. 
When it was over, this city of 50,000 would reel, broken and bloodied.

For the watching world, the image of the hollowed shell of St. John?s
was ground zero. For 115 years, the hospital system created by the
Catholic order of the Sisters of Mercy healed the community?s illnesses
and injuries. In seconds, the nine-story symbol of the city?s strength
and caring, built and expanded since 1968, would stand gravely injured
itself. 
Yet in many ways, the story of what happened inside the walls of St.
John?s might better stand as a microcosm of the horrors, heroism and
humanity that played out across Joplin that night. 
Witness upon witness recounts a stream of ?walking wounded,?
individuals impaled by wood, glass or metal, limbs missing, flesh torn
from their bodies, lurching toward the hospital. Many remain haunted by
the carnage they saw. 
But that night at the hospital, they said, also will be remembered as
one of the city?s proudest. 
There was the surgeon who operated by flashlight as the hospital
crashed around him. The ER doc who plunged a chest tube through the ribs
of a young man to keep him from dying. 
Nurses used their bodies to blanket vulnerable patients from
wind-hurled debris. A floor tech plucked a flying man from the air.
Employees wielded axes to free drugs from locked cabinets. 
And then there were the strangers. Hundreds rushed in convoys of pickup
trucks, descending on the hospital to speed the wounded away. 
Hospital visitors left the sides of their dead and dying loved ones to
carry fragile patients down blackened hallways, guided by the dim light
of cell phones. 
?We did what we had to do,? said John Seay. 
? ? ? 
At 5:41 p.m., the tornado descends, a black, twisting wall on the
western horizon. It splinters houses, strips trees, heaves cars.
Hailstones crash through glass like sledgehammers. Rain pounds down in a
stinging curtain. 
Minutes away, St. John?s waits ? 183 patients in its 367 licensed beds;
some 25 patients in the ER; 100 staff on duty; an unknown number of
visitors in the patient rooms, halls and waiting areas. 
In the ER, Angie Abner, 40 ? a paramedic who became a nurse only a year
ago, and who has missed work the last two days because of food poisoning
? has been doing triage. Now, minutes after the call Execute Condition
Gray, she is struggling to get patients to safety, into hallways and
away from windows. 
People, having grown too used to these warnings and then seeing storms
peter out, refuse to move. 
?Folks, this is for your own safety,? Abner belts, emphatic. ?You have
to listen to me!? 
A man waves her off and tries to leave. 
?No, sir!? she snaps. ?You are my responsibility and you?re not going
anywhere!? 
Upstairs, in the third-floor intensive-care unit, nurse Tammy Fritchey,
a 27-year-veteran, places blankets and pillows over patients who are too
sick to move, the ones on ventilators. With two other nurses, she will
soon huddle in an interior office. 
?God,? she?ll pray silently, holding tight to another nurse. ?Please
help our patients.? 
On other floors, patients able to walk are placed in hardback chairs or
wheelchairs and lined up down the hallways. Others are rolled out in
their beds. 
Condition Grays are practiced as drills at least a dozen times a year.
All hospitals, as part of their accreditation, have emergency codes and
plans for storms, abducted babies, gunmen on the premises. 
But this is no drill. 
In Room 587, Connie Lansdown, 62, has just taken a shower. Weak and
recovering from a hysterectomy, a treatment for possible cancer, she
wears a hospital gown, a robe and flip-flops. 
Below, near Room 381, the mechanic John Seay stands in the hall next to
his mother?s bed. She?s on oxygen and has been visiting with more than a
dozen relatives all afternoon. Seay is calm, sure nothing will happen. 
So is Belinda Pfeiffer, 58, who with her brother Rodney Stover, 60, is
in the hallway with their 85-year-old father, Ralph Stover. He?s to be
released the next day into rehab after complications from a recent
corneal transplant. Pfeiffer feels so calm she feeds her dad spoonfuls
of pureed beef and green beans near Room 386. 
In the eighth-floor obstetrics unit, fathers are close by their newborn
babies. In the operating room, surgeon Dusty Smith, wearing a sterile
mask and baby-blue scrubs, is just about to drain a woman?s infected hip
joint. The woman is anesthetized. His scalpel is at work. 
Near the ER?s glass doors, Kevin Kikta, 40, one of only two emergency
physicians on duty, stands with a patient?s chart. He looks up and sees
a security guard tearing down the corridor. 
?Take cover!? the guard shouts. ?We?re gonna get hit!? 
? ? ? 
Wind roars with such force the steel beams supporting the hospital?s
top floors twist four inches. 
Glass explodes from every window; the air turns cold; lights flicker
and die. The building jolts and is cloaked in blackness. Both
generators, main and backup, have been blasted from their foundations. 
Water pipes burst, showering everything. Ceilings cave; wires hang in
the air like spider webs and spill on the floor. Explosive natural gas
spews from broken pipes on the lower floors. 
The wind?s power is tremendous. Connie Lansdown, the hysterectomy
patient, watches the storm yank a man from a reclining chair and drag
him down the hall. 
?This is no place to be!? she thinks, hurrying into a windowless room
and pressing her shoulder against the door. 
X-ray machines, respirators, computer monitors and doors ripped from
their hinges ricochet down the halls, spin and crash through the air. 
Fathers in the nursery drape themselves over their newborns. 
In her bed, John Seay?s mother is being sucked down the corridor.
Knocked to a knee, he covers her with one arm. 
?I gotcha,? he says. 
He drives his foot into the floor to stop the movement, but can?t. They
sail down the hall and crash. 
He looks at his mother. Her eyes are shut; her oxygen mask is still on.
She looks OK. 
?I?m going to check on the others,? he tells her and leaves to search
for their relatives. 
Outside the ER, the wind catapults a man through the emergency unit?s
blown-out entryway, where St. John?s floor tech Ben Graskemper, 31, a
volunteer firefighter as imposing as a bouncer, snatches him from the
air and shields him beneath his body. 
From gu.lang <@t> gmx.at  Fri Jun 24 09:24:37 2011
From: gu.lang <@t> gmx.at (Gudrun Lang)
Date: Fri Jun 24 09:31:22 2011
Subject: AW: [Histonet] Tissue Shriveling in Paraffin
In-Reply-To: <4E044A71.5876.00D9.0@auburn.edu>
References: <4E044A71.5876.00D9.0@auburn.edu>
Message-ID: <40705B64685245BFB0E0878FA2BB9142@dielangs.at>

That sounds like insufficient infiltration with paraffin.
Melt your block again and let it sit in paraffin for a few hours or even
over night.
Most time this helps to get better sections.
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Michelle
Aono
Gesendet: Freitag, 24. Juni 2011 15:28
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Tissue Shriveling in Paraffin

I was cutting some bone/joint tissue and noticed that the cartilaginous
portion was concave/indented, instead of flush with the rest of the
block surface.  Even as I continued to cut that portion always seemed a
little sunken into the block face and all the sections crumbled.  I
didn't seal the block after I was done and when I came back the next day
the entire tissue sample was shriveled and pulling away from the
paraffin.  I'm new, but in the few bone sections I've done I've never
had this happen!  Any ideas?

~Shelly

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From PMonfils <@t> Lifespan.org  Fri Jun 24 10:23:53 2011
From: PMonfils <@t> Lifespan.org (Monfils, Paul)
Date: Fri Jun 24 10:24:13 2011
Subject: [Histonet] Sirius Red Stain
In-Reply-To: 
References: 
Message-ID: <4EBFF65383B74D49995298C4976D1D5E084A20C6@LSRIEXCH1.lsmaster.lifespan.org>

I make my own solution, 0.1% in saturated aqueous picric acid.



From jcox90 <@t> yahoo.com  Fri Jun 24 10:49:22 2011
From: jcox90 <@t> yahoo.com (jcox90@yahoo.com)
Date: Fri Jun 24 10:49:24 2011
Subject: [Histonet] Looking for 2-3 week temp Histology assignment in July
Message-ID: <1308930562.35432.YahooMailRC@web161619.mail.bf1.yahoo.com>

Hi Histonetters!
I am looking for a temp histology assignment for a couple of weeks in July. I am 
HT (ascp) and have 17 years experience. Please respond to this email if you are 
looking for some help, thank you, Jill
 
Jill Cox, HT ASCP
From member <@t> linkedin.com  Fri Jun 24 10:52:26 2011
From: member <@t> linkedin.com (Adekunle Oluwatosin Adeluwoye via LinkedIn)
Date: Fri Jun 24 10:52:29 2011
Subject: [Histonet] Invitation to connect on LinkedIn
Message-ID: <1408097674.5332261.1308930746368.JavaMail.app@ela4-bed82.prod>

LinkedIn
------------




    Adekunle Oluwatosin Adeluwoye requested to add you as a connection on LinkedIn:
  
------------------------------------------

David,

I'd like to add you to my professional network on LinkedIn.

- Adekunle Oluwatosin

Accept invitation from Adekunle Oluwatosin Adeluwoye
http://www.linkedin.com/e/yvpgd1-gpbbkjr1-3/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I123429292_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYOej8VczgPcz59bRhktn1vsk5CbPkUc3cPe3wOcPcLrCBxbOYWrSlI/EML_comm_afe/

View invitation from Adekunle Oluwatosin Adeluwoye
http://www.linkedin.com/e/yvpgd1-gpbbkjr1-3/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I123429292_13/3cNnP8VczAOd3cOckALqnpPbOYWrSlI/svi/ 
------------------------------------------

DID YOU KNOW LinkedIn can help you find the right service providers using recommendations from your trusted network? Using LinkedIn Services, you can take the risky guesswork out of selecting service providers by reading the recommendations of credible, trustworthy members of your network. 
http://www.linkedin.com/e/yvpgd1-gpbbkjr1-3/svp/inv-25/

 
-- 
(c) 2011, LinkedIn Corporation
From member <@t> linkedin.com  Fri Jun 24 10:59:36 2011
From: member <@t> linkedin.com (Adekunle Oluwatosin Adeluwoye via LinkedIn)
Date: Fri Jun 24 10:59:39 2011
Subject: [Histonet] Invitation to connect on LinkedIn
Message-ID: <465078021.5355874.1308931176218.JavaMail.app@ela4-bed36.prod>

LinkedIn
------------




    Adekunle Oluwatosin Adeluwoye requested to add you as a connection on LinkedIn:
  
------------------------------------------

David,

I'd like to add you to my professional network on LinkedIn.

- Adekunle Oluwatosin

Accept invitation from Adekunle Oluwatosin Adeluwoye
http://www.linkedin.com/e/yvpgd1-gpbbtrfa-4r/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I123431533_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYPcPkNcPgPcz59bRhktn1vsk5CbPkUc3cPe3wOcPcLrCBxbOYWrSlI/EML_comm_afe/

View invitation from Adekunle Oluwatosin Adeluwoye
http://www.linkedin.com/e/yvpgd1-gpbbtrfa-4r/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I123431533_13/3cNnPcPdj4Pd3cOckALqnpPbOYWrSlI/svi/ 
------------------------------------------

DID YOU KNOW LinkedIn can help you find the right service providers using recommendations from your trusted network? Using LinkedIn Services, you can take the risky guesswork out of selecting service providers by reading the recommendations of credible, trustworthy members of your network. 
http://www.linkedin.com/e/yvpgd1-gpbbtrfa-4r/svp/inv-25/

 
-- 
(c) 2011, LinkedIn Corporation
From CThornton <@t> dahlchase.com  Fri Jun 24 11:08:02 2011
From: CThornton <@t> dahlchase.com (Clare Thornton)
Date: Fri Jun 24 11:08:11 2011
Subject: [Histonet] Formula R paraffin
Message-ID: 

Does anyone have any experience with Formula R paraffin, from Leica, good or bad?  We currently use Paraplast X-tra but occasionally have troubles with compression, and we heard Formula R is harder and less likely to compress.



Thanks!


Clare J. Thornton, HTL(ASCP), QIHC
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthornton@dahlchase.com

From rjbuesa <@t> yahoo.com  Fri Jun 24 11:21:35 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Fri Jun 24 11:21:38 2011
Subject: [Histonet] Formula R paraffin
In-Reply-To: 
Message-ID: <851084.79705.qm@web65712.mail.ac4.yahoo.com>

If sometimes you are experiencing compression problems it could be because your paraffin has some xylene contamination, or your blocks are not cold enough when cutting, or you are using a paraffin of lower melting point as needed for the tissue you regularly process.
Paraplast X-tra always worked fine for me. Check with them for a harder paraffin instead of changing to a different paraffin altogether.
Ren? J.

--- On Fri, 6/24/11, Clare Thornton  wrote:


From: Clare Thornton 
Subject: [Histonet] Formula R paraffin
To: "'Histonet@lists.utsouthwestern.edu'" 
Date: Friday, June 24, 2011, 12:08 PM


Does anyone have any experience with Formula R paraffin, from Leica, good or bad?? We currently use Paraplast X-tra but occasionally have troubles with compression, and we heard Formula R is harder and less likely to compress.



Thanks!


Clare J. Thornton, HTL(ASCP), QIHC
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthornton@dahlchase.com

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From mjdessoye <@t> wvhcs.org  Fri Jun 24 11:26:46 2011
From: mjdessoye <@t> wvhcs.org (Dessoye, Michael J)
Date: Fri Jun 24 11:26:54 2011
Subject: [Histonet] HistoGel
Message-ID: 

Hello,
 
Does anyone out there have any experience with HistoGel?  It's Richard Allan/Thermo Fisher.  They claim that you can "embed" scant tissues in the gel and then process, embed, and cut as usual.  Just wondering how it works in the real world....
 
Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org   |
575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

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From shultz11 <@t> cox.net  Fri Jun 24 11:28:55 2011
From: shultz11 <@t> cox.net (Kendra)
Date: Fri Jun 24 11:35:25 2011
Subject: [Histonet] (no subject)
Message-ID: <723pa4ptd019lmddnpqhrxwt.1308932935185@email.android.com>

to
From kmilne <@t> bccancer.bc.ca  Fri Jun 24 12:27:09 2011
From: kmilne <@t> bccancer.bc.ca (Milne, Katy)
Date: Fri Jun 24 12:27:15 2011
Subject: [Histonet] RE: HistoGel
In-Reply-To: <82fc8dae-dfdf-46ae-bfaa-a86d4092b2f0@SRVEXHT01.phsabc.ehcnet.ca>
References: <82fc8dae-dfdf-46ae-bfaa-a86d4092b2f0@SRVEXHT01.phsabc.ehcnet.ca>
Message-ID: <3FEFF18FF4E1914A9AB7D8498591BE8610CF058850@VEXCCR02.phsabc.ehcnet.ca>

We use histogel a lot in our lab.  It's a research lab and we use it for a few purposes - pelleting cultured cells then creating multi-culture TMAs for testing antibodies and also pelleting cells from ascites and pleural effusions.  Has also been used to process really small samples that could have been lost in the processor through the cassettes.

Works quite well.  The researchers just put the samples in histogel and give it to me in formalin then I process it as I would regular tissue.  Cuts very well too.

Katy


Message: 3
Date: Fri, 24 Jun 2011 12:26:46 -0400
From: "Dessoye, Michael J" 
Subject: [Histonet] HistoGel
To: 
Message-ID:
	
Content-Type: text/plain;	charset="iso-8859-1"

Hello,
 
Does anyone out there have any experience with HistoGel?  It's Richard Allan/Thermo Fisher.  They claim that you can "embed" scant tissues in the gel and then process, embed, and cut as usual.  Just wondering how it works in the real world....
 
Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org   |
575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 

From amosbrooks <@t> gmail.com  Fri Jun 24 12:29:09 2011
From: amosbrooks <@t> gmail.com (Amos Brooks)
Date: Fri Jun 24 12:29:13 2011
Subject: [Histonet] Histogel Problem
Message-ID: 

Hi,
   I have a problem with some blocks that were prepared with Histogel. I was
hoping someone else might have had a similar problem and figured it out. I
took a photo of the blocks that were mads and put them in a Picassa album
here:
https://picasaweb.google.com/lh/photo/APO3HsIMa2_jPOEs3QRGUjhz3qi22FNPb2i5JJnBCAk?feat=directlink
   The long & short of it is that the blocs were prepared by the researcher
for me to process. They are mouse kidneys. Now it is entirely possible for
him to have goofed something up in preparing the Histogel blocks. I wasn't
there when he did it, but when I looked at them before processing, they all
looked fine. (Like the adjacent good one in the photo). When they were
processed they were placed in the VIP right next to each other. When I went
to embed them this morning all but one of the four looked fine. The one that
didn't come out well looked like the Histogel had shrunk up and shriveled
around the kidneys. I am sure this will be aweful to cut, and the researcher
is going to have a bird over it since this happened with another project
previously. I would like to have a decent explanation for him, so if anyone
knows what might have happened and has suggestions I would love to hear it
(yes vendors too are welcome to answer this of course!).
   By the way, this was processed on a rather short cycle of 15-20 min per
station of graded ETOH from 70% to 100% with 3 xylene stations and 4
paraffin stations (45 min for these). It seems fine for everything else that
was on the processor. Just that one Histogel block was the issue.

Thank you,
Amos
From Delossantos_Roseann <@t> Allergan.com  Fri Jun 24 12:47:03 2011
From: Delossantos_Roseann <@t> Allergan.com (Delossantos_Roseann)
Date: Fri Jun 24 12:47:21 2011
Subject: [Histonet] Histogel Problem
In-Reply-To: 
References: 
Message-ID: <6E159393AFE18142A83CE7C2475AD96D0916498C5A@AGMAILCL100.allergan.com>

Hi,
I've encountered this problem before in my previous lab.  To reduce the Histogel from shrinking that badly, avoid putting the tissue at the very edge of the Histogel, space them out nicely in the middle, providing sufficient amount of extra Histogel between each tissue and surrounding them.  A little bit of shrinking usually do not interfere with cutting as long as the whole thing is placed flat on the final paraffin block, the rest will stretch out on the water bath.  I find sometimes the gel does not behave well in the water but as long as it is not on top of your tissue when placed on your slide, it should interfere much with staining.


Rose



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks
Sent: Friday, June 24, 2011 10:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histogel Problem

Hi,
   I have a problem with some blocks that were prepared with Histogel. I was
hoping someone else might have had a similar problem and figured it out. I
took a photo of the blocks that were mads and put them in a Picassa album
here:
https://picasaweb.google.com/lh/photo/APO3HsIMa2_jPOEs3QRGUjhz3qi22FNPb2i5JJnBCAk?feat=directlink
   The long & short of it is that the blocs were prepared by the researcher
for me to process. They are mouse kidneys. Now it is entirely possible for
him to have goofed something up in preparing the Histogel blocks. I wasn't
there when he did it, but when I looked at them before processing, they all
looked fine. (Like the adjacent good one in the photo). When they were
processed they were placed in the VIP right next to each other. When I went
to embed them this morning all but one of the four looked fine. The one that
didn't come out well looked like the Histogel had shrunk up and shriveled
around the kidneys. I am sure this will be aweful to cut, and the researcher
is going to have a bird over it since this happened with another project
previously. I would like to have a decent explanation for him, so if anyone
knows what might have happened and has suggestions I would love to hear it
(yes vendors too are welcome to answer this of course!).
   By the way, this was processed on a rather short cycle of 15-20 min per
station of graded ETOH from 70% to 100% with 3 xylene stations and 4
paraffin stations (45 min for these). It seems fine for everything else that
was on the processor. Just that one Histogel block was the issue.

Thank you,
Amos
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail, including any attachments, is meant only for the intended recipient and may be a confidential communication or a communication privileged by law. If you received this e-mail in error, any review, use, dissemination, distribution, or copying of this e-mail is strictly prohibited. Please notify the sender immediately of the error by return e-mail and please delete this message from your system. Thank you in advance for your cooperation.

From Dorothy.L.Webb <@t> HealthPartners.Com Fri Jun 24 14:20:33 2011 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Jun 24 14:20:38 2011 Subject: [Histonet] bluing Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD7D@HPEMX3.HealthPartners.int> Looking to change my "bluing" step in the H&E process to obtain a bluer (less purple) hue to the nuclear detail. What is everyone using in their bluing step?? Thanks for all of your ideas!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From rjbuesa <@t> yahoo.com Fri Jun 24 14:35:18 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 24 14:35:22 2011 Subject: [Histonet] bluing In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD7D@HPEMX3.HealthPartners.int> Message-ID: <201322.12289.qm@web65716.mail.ac4.yahoo.com> There are several "bluing solutions" in the market, or you could use lithium carbonate at different concentrations until you find one of your liking. Ren? J. --- On Fri, 6/24/11, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] bluing To: "'histonet@lists.utsouthwestern.edu'" Date: Friday, June 24, 2011, 3:20 PM Looking to change my "bluing" step in the H&E process to obtain a bluer (less purple) hue to the nuclear detail.? What is everyone using in their bluing step?? Thanks for all of your ideas!! ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sandra.Harrison3 <@t> va.gov Fri Jun 24 15:33:12 2011 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Fri Jun 24 15:33:18 2011 Subject: [Histonet] bluing In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD7D@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD7D@HPEMX3.HealthPartners.int> Message-ID: Hi Dorothy, Try Richard Allan Bluing Reagent. Here's what they say about their product: "It is a buffered product that ensures the proper alkalinity (pH=8.0). Unlike ammonia and lithium carbonate, RA's Bluing Reagent does not allow for the pH shift which can affect the crispness of nuclear detail." When I first began supervising this lab 5 years ago, they "made from scratch" their own hematoxylin and eosin (not to mention buffered formalin.) Unfortunately, the quality of the stain was very spotty and caused the Pathologists a lot of problems. I switched us to the Richard Allan 7211 Hematoxylin, which has beautiful, crisp nuclear detail. We also went with the recommended Richard Allan Clarifier, Bluing and Eosin, as well, so that we could produce a consistently high quality H&E every time. In 5 years, we've had very few complaints about the stain from the 8-10 Pathologists we've worked with, except for one occasion when there was some isolated nuclear hazing. We did some detective work and determined that the cause was due to a rack or two that had been placed in the oven without properly removing the excess water or draining of the slides before placing them in the oven. Have a great week-end everybody. It's practically the 1st sunny day we've had, here in Minneapolis, for the past 2 weeks and Saturday and Sunday's forecast looks good, too! Sandy Harrison VA-Minneapolis Supervisor, Anatomical and Surgical Pathology 612-467-2449 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, June 24, 2011 2:21 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bluing Looking to change my "bluing" step in the H&E process to obtain a bluer (less purple) hue to the nuclear detail. What is everyone using in their bluing step?? Thanks for all of your ideas!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Fri Jun 24 15:53:39 2011 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Jun 24 15:53:43 2011 Subject: [Histonet] blades Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD81@HPEMX3.HealthPartners.int> Trying to clean up some things hanging out there in our lab and wondering what everyone does with a blade that has been used minimally and tech done for the day with the microtome. Where do you store that blade for use tomorrow or do you toss and not worry about the cost involved? I do not like them sitting on top of the microtome. Any good ideas?? Thanks, as always! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From smcbride <@t> andrew.cmu.edu Fri Jun 24 16:16:06 2011 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Fri Jun 24 16:16:14 2011 Subject: [Histonet] blades In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD81@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD81@HPEMX3.HealthPartners.int> Message-ID: <0FA5973D-A2CA-4364-BD5E-A477F302BD8D@andrew.cmu.edu> Dorothy, I put ours in a 15 mL centrifuge tube with a cap & sit it on the base of the microtome for the next use, that way, no one gets cut & the blade is able to be used to the fullest of it's potential. :-) Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu On Jun 24, 2011, at 4:53 PM, Webb, Dorothy L wrote: > Trying to clean up some things hanging out there in our lab and wondering what everyone does with a blade that has been used minimally and tech done for the day with the microtome. Where do you store that blade for use tomorrow or do you toss and not worry about the cost involved? I do not like them sitting on top of the microtome. Any good ideas?? Thanks, as always! > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From epeters2 <@t> gmu.edu Fri Jun 24 16:10:30 2011 From: epeters2 <@t> gmu.edu (Esther C Peters) Date: Fri Jun 24 16:19:14 2011 Subject: [Histonet] blades In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD81@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD81@HPEMX3.HealthPartners.int> Message-ID: We put ours in a small slide box (plastic or styrofoam, 5-25 slides) that is clearly marked "Microtome Blades for Facing Blocks" to be used another day. Esther C. Peters, Ph.D. Assistant Professor Department of Environmental Science & Policy Biology Program/Medical Technology Coordinator George Mason University 4400 University Drive, MSN 5F2 Fairfax, VA 22030-4444 Office: David King Hall 3057 Phone: 703-993-3462 Fax: 703-993-1066 epeters2@gmu.edu ----- Original Message ----- From: "Webb, Dorothy L" Date: Friday, June 24, 2011 4:53 pm Subject: [Histonet] blades > Trying to clean up some things hanging out there in our lab and > wondering what everyone does with a blade that has been used > minimally and tech done for the day with the microtome. Where do > you store that blade for use tomorrow or do you toss and not worry > about the cost involved? I do not like them sitting on top of the > microtome. Any good ideas?? Thanks, as always! > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and > are intended solely for the use of the individual or entity to > whom they are addressed. If you are not the intended recipient or > the individual responsible for delivering the e-mail to the > intended recipient, please be advised that you have received this > e-mail in error and that any use, dissemination, forwarding, > printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately > notify the HealthPartners Support Center by telephone at (952) 967- > 6600. You will be reimbursed for reasonable costs incurred in > notifying us. HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bakevictoria <@t> gmail.com Fri Jun 24 16:31:33 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Jun 24 16:31:38 2011 Subject: [Histonet] blades In-Reply-To: References: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD81@HPEMX3.HealthPartners.int> Message-ID: I use the cardboards that come in a box of slides. A small piece of tape on the open side and mark it used. I just always make sure I have the blade edge facing the folded part. I know some who will tape this folded board it to the side of their microtome and use it as a trimming blade holder. Vikki On Fri, Jun 24, 2011 at 5:10 PM, Esther C Peters wrote: > We put ours in a small slide box (plastic or styrofoam, 5-25 slides) that > is clearly marked "Microtome Blades for Facing Blocks" to be used another > day. > > Esther C. Peters, Ph.D. > Assistant Professor > Department of Environmental Science & Policy > Biology Program/Medical Technology Coordinator > George Mason University > 4400 University Drive, MSN 5F2 > Fairfax, VA 22030-4444 > Office: David King Hall 3057 > Phone: 703-993-3462 > Fax: 703-993-1066 > epeters2@gmu.edu > > ----- Original Message ----- > From: "Webb, Dorothy L" > Date: Friday, June 24, 2011 4:53 pm > Subject: [Histonet] blades > > > Trying to clean up some things hanging out there in our lab and > > wondering what everyone does with a blade that has been used > > minimally and tech done for the day with the microtome. Where do > > you store that blade for use tomorrow or do you toss and not worry > > about the cost involved? I do not like them sitting on top of the > > microtome. Any good ideas?? Thanks, as always! > > > > > > > > ________________________________ > > This e-mail and any files transmitted with it are confidential and > > are intended solely for the use of the individual or entity to > > whom they are addressed. If you are not the intended recipient or > > the individual responsible for delivering the e-mail to the > > intended recipient, please be advised that you have received this > > e-mail in error and that any use, dissemination, forwarding, > > printing, or copying of this e-mail is strictly prohibited. > > > > If you have received this e-mail in error, please immediately > > notify the HealthPartners Support Center by telephone at (952) 967- > > 6600. You will be reimbursed for reasonable costs incurred in > > notifying us. HealthPartners R001.0 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jmacdonald <@t> mtsac.edu Fri Jun 24 16:50:43 2011 From: jmacdonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Jun 24 16:50:58 2011 Subject: [Histonet] blades In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD81@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD81@HPEMX3.HealthPartners.int> Message-ID: <9A2E9444-36FF-418C-A794-AB6531E953AD@mtsac.edu> We use plastic 5 slide mailers Sent from my iPhone On Jun 24, 2011, at 1:53 PM, "Webb, Dorothy L" wrote: > Trying to clean up some things hanging out there in our lab and wondering what everyone does with a blade that has been used minimally and tech done for the day with the microtome. Where do you store that blade for use tomorrow or do you toss and not worry about the cost involved? I do not like them sitting on top of the microtome. Any good ideas?? Thanks, as always! > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ervelazquez <@t> gmail.com Fri Jun 24 18:56:32 2011 From: ervelazquez <@t> gmail.com (Eric Velazquez) Date: Fri Jun 24 18:56:36 2011 Subject: [Histonet] HT Position - Irvine, CA Message-ID: Hi Histonet, We are currently looking for a full-time Histotechnician (preferably HT certified) with experience in IHC. Please read the description below and if you're interested please submit your resume via e-mail to: * eric.velazquez@agendia.com*. *ESSENTIAL DUTIES AND RESPONSIBILITIES* - Performs histology aspects of the lab, which includes cutting tissue(frozen, FFPE), manually staining sections for H&E imaging, IHC staining, loading slides on ScanScope for imaging and scoring by pathologist - Maintains laboratory logs so that current information is complete and readily available to staff, including clinical log sheet, ScanScope identification - Performs all aspects of lab support to ensure timely completion of samples. Reports any discrepancies to the Director Of Laboratory Operations. - Performs various maintenance and/or housekeeping tasks to keep laboratory clean, and ensure ease of use. - Cleans and fills water baths, bulk reagents, dump waste containers - Reports any malfunctions of equipment to the Director Of Laboratory Operations. - Washes all laboratory dishes to ensure a clean supply when needed. - Performs data entry tasks in the laboratory and office when needed. - Follows all Agendia, Inc.?s health and safety policies and procedures - Performs other related duties as required or assigned Thank you for your interest. -Eric Velazquez Agendia Inc. From lpwenk <@t> sbcglobal.net Sat Jun 25 09:10:17 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Jun 25 09:10:21 2011 Subject: [Histonet] HT Position - Irvine, CA In-Reply-To: References: Message-ID: <803AB1A1F69C40708BC7795115D1CFB7@HP2010> Where are you? City, State, Country, Lab, etc. Info, please. -----Original Message----- From: Eric Velazquez Sent: Friday, June 24, 2011 7:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT Position - Irvine, CA Hi Histonet, We are currently looking for a full-time Histotechnician (preferably HT certified) with experience in IHC. Please read the description below and if you're interested please submit your resume via e-mail to: * eric.velazquez@agendia.com*. *ESSENTIAL DUTIES AND RESPONSIBILITIES* - Performs histology aspects of the lab, which includes cutting tissue(frozen, FFPE), manually staining sections for H&E imaging, IHC staining, loading slides on ScanScope for imaging and scoring by pathologist - Maintains laboratory logs so that current information is complete and readily available to staff, including clinical log sheet, ScanScope identification - Performs all aspects of lab support to ensure timely completion of samples. Reports any discrepancies to the Director Of Laboratory Operations. - Performs various maintenance and/or housekeeping tasks to keep laboratory clean, and ensure ease of use. - Cleans and fills water baths, bulk reagents, dump waste containers - Reports any malfunctions of equipment to the Director Of Laboratory Operations. - Washes all laboratory dishes to ensure a clean supply when needed. - Performs data entry tasks in the laboratory and office when needed. - Follows all Agendia, Inc.?s health and safety policies and procedures - Performs other related duties as required or assigned Thank you for your interest. -Eric Velazquez Agendia Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amarquez29 <@t> yahoo.com Sat Jun 25 09:16:32 2011 From: amarquez29 <@t> yahoo.com (Aurea Marquez) Date: Sat Jun 25 09:16:42 2011 Subject: [Histonet] Contents of Histonet digest Message-ID: <462293.86417.qm@web120511.mail.ne1.yahoo.com> ? Aurea Griselle Marquez ________________________________ From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Fri, June 24, 2011 1:01:21 PM Subject: Histonet Digest, Vol 91, Issue 33 Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. Formula R paraffin (Clare Thornton) ? 2. Re: Formula R paraffin (Rene J Buesa) ? 3. HistoGel (Dessoye, Michael J) ? 4. (no subject) (Kendra) ---------------------------------------------------------------------- Message: 1 Date: Fri, 24 Jun 2011 12:08:02 -0400 From: Clare Thornton Subject: [Histonet] Formula R paraffin To: "'Histonet@lists.utsouthwestern.edu'" ??? Message-ID: ??? Content-Type: text/plain; charset="us-ascii" Does anyone have any experience with Formula R paraffin, from Leica, good or bad?? We currently use Paraplast X-tra but occasionally have troubles with compression, and we heard Formula R is harder and less likely to compress. Thanks! Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com ------------------------------ Message: 2 Date: Fri, 24 Jun 2011 09:21:35 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Formula R paraffin To: "'Histonet@lists.utsouthwestern.edu'" ??? , ??? Clare Thornton ??? Message-ID: <851084.79705.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If sometimes you are experiencing compression problems it could be because your paraffin has some xylene contamination, or your blocks are not cold enough when cutting, or you are using a paraffin of lower melting point as needed for the tissue you regularly process. Paraplast X-tra always worked fine for me. Check with them for a harder paraffin instead of changing to a different paraffin altogether. Ren? J. --- On Fri, 6/24/11, Clare Thornton wrote: From: Clare Thornton Subject: [Histonet] Formula R paraffin To: "'Histonet@lists.utsouthwestern.edu'" Date: Friday, June 24, 2011, 12:08 PM Does anyone have any experience with Formula R paraffin, from Leica, good or bad?? We currently use Paraplast X-tra but occasionally have troubles with compression, and we heard Formula R is harder and less likely to compress. Thanks! Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 24 Jun 2011 12:26:46 -0400 From: "Dessoye, Michael J" Subject: [Histonet] HistoGel To: Message-ID: ??? Content-Type: text/plain;??? charset="iso-8859-1" Hello, Does anyone out there have any experience with HistoGel?? It's Richard Allan/Thermo Fisher.? They claim that you can "embed" scant tissues in the gel and then process, embed, and cut as usual.? Just wondering how it works in the real world.... Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org ? | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ------------------------------ Message: 4 Date: Fri, 24 Jun 2011 11:28:55 -0500 From: Kendra Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: <723pa4ptd019lmddnpqhrxwt.1308932935185@email.android.com> Content-Type: text/plain; charset=utf-8 From lpwenk <@t> sbcglobal.net Sat Jun 25 09:26:23 2011 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Jun 25 09:26:27 2011 Subject: [Histonet] bluing In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD7D@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD7D@HPEMX3.HealthPartners.int> Message-ID: <2B7338AFC93A4F91AD215CDC80E1B201@HP2010> It might just need longer time in the bluing agent. Hematoxylin solutions stain the nuclei reddish (look at your slide right after coming out of hematoxylin - tissue will look reddish). The alkaline solutions that are used for "bluing" remove a H+ group on the aluminum hematein (that's the staining chemical of the hematoxylin solution), and change it to a -OH group. This changes the aluminum hematein from a reddish color to a blue color. The more alkaline (higher pH) the bluing agent, the faster this reaction and color change, and the less time is needed in the bluing agent. The lower the alkalinity (not as high pH but still in the alkaline range) the bluing agent, the slower the reaction and color change, and more time is needed in the bluing agent. Dilute ammonia water usually only takes a few seconds, as it's pH is usually high (pH 10). Tap water can have a pH of around 7, and may take 5-10 minutes to "blue". If the tap water is more acidic (pH 5 or below), the slides may not "blue". Now, we have to get all the nuclei from the reddish color to the bluish color. If the slide is not in the bluing agent for enough time (for the type/pH of bluing agent), then some of the nuclei change to blue, while some still remain reddish (or within a single nucleus, some of the DNA has changed to blue, some remains reddish), hence a more purple color. So, the easiest thing to try right now is to stain 2 slides from the same block (serial sections) in your hematoxylin for the same time, put one in the bluing agent for the usual time, put the other in the alkaline solution for extended time, and see if the nuclei on the second slide are now more blue than the first slide. Let us know. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: Webb, Dorothy L Sent: Friday, June 24, 2011 3:20 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bluing Looking to change my "bluing" step in the H&E process to obtain a bluer (less purple) hue to the nuclear detail. What is everyone using in their bluing step?? Thanks for all of your ideas!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sadey <@t> hotmail.ca Sat Jun 25 11:00:44 2011 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Sat Jun 25 11:00:48 2011 Subject: [Histonet] Leica Bond for IHCs Message-ID: Hello netters: Looking for opinions on the Leica Bond immuno stainer please. Thanks. Sheila From gayle.callis <@t> bresnan.net Sat Jun 25 11:47:15 2011 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Sat Jun 25 11:47:30 2011 Subject: [Histonet] RE: Bluing Message-ID: <000101cc3357$8b945ff0$a2bd1fd0$@callis@bresnan.net> Dear All, We use commercial bluing reagents for convenience e.g. Richard Allan Bluing Reagent ready to use and have used Scotts Tap Water Substitute Concentrate diluted before use. We do not use ammonia water anymore as the high pH can cause section lift off from slide. Here is recipe for Scotts. SCOTT'S TAP WATER SUBSTITUTE (You can make this up as a 10X concentrate if you wish. Store in house concentrate at 4C or growth will occur) Sodium bicarbonate 2 g Magnesium sulfate (anhydrous) 20 g Tap Water 1 L Peggy presented a good explanation of bluing. We do not use tap water anymore due to incorrect pH. One of the failures for complete bluing is not changing the bluing reagent daily with large volumes of slides. Lower number of slides, one might be able to get by with fewer changes of bluing. Tap water rinses dilutes the reagent and can change the pH, which should be pH 8. If there is a large volume of slides, anything over 75 or more, we change the bluing reagent daily. It is important to remember that Clarifiers e.g. acetic acid reagents used with progressive hematoxylins (Richard Allan Hematoxylin 1, Gill 1,2,3) will suffer from tap water rinse dilution too. We also change those, and if Clarifier looks rather yellow, then you have probably exhausted the acetic acid and its effectiveness. Jerry Fredenburgh has given excellent workshops on hematoxylin staining in the past and passed on many of these facts. Consequently, we have no clarifying or bluing problems. Fredenburgh recommendations for Richard Allan Hematoxylin 1 staining procedure that we use: Hematoxylin 1 - 1.5 min, longer if needed Tap water rinse-1 minute Clarifier - 1 minute Tap water rinse - 1 minute Bluing - 1 minute Tap water rinse - 1 minute Remember, static or non-running water rinse stations are contaminated with carryover hematoxylin, clarifier and/or bluing reagent. If you do NOT remove the bluing reagent with a good rinse, then eosin staining is affected by cations carried over from the bluing reagent. We also have an alcohol step (matching % of alcohol in eosin) before going into this stain. This helps remove the cations plus prepares the section for eosin staining. Perform good rinses and change static water rinse stations after a run, or frequently during a day of heavy staining. The second and third racks of slides on a stainer should have the same tap water rinse conditions as the first rack. Yes, extra work but you need to ensure consistent staining results of H&E of ALL sections- the universal workhorse stain we depend on so heavily. Years ago, Richard Allan had an excellent manual for H&E staining guidelines on how to perform and adjust your H&E staining. Sadly the manual is no longer published but the good news is ...... I saved my copy, and scanned it to pdf if anyone wants it. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT **************************************************************************** ***************************************************************** Peggy Wenk wrote: It might just need longer time in the bluing agent. Hematoxylin solutions stain the nuclei reddish (look at your slide right after coming out of hematoxylin - tissue will look reddish). The alkaline solutions that are used for "bluing" remove a H+ group on the aluminum hematein (that's the staining chemical of the hematoxylin solution), and change it to a -OH group. This changes the aluminum hematein from a reddish color to a blue color. The more alkaline (higher pH) the bluing agent, the faster this reaction and color change, and the less time is needed in the bluing agent. The lower the alkalinity (not as high pH but still in the alkaline range) the bluing agent, the slower the reaction and color change, and more time is needed in the bluing agent. Dilute ammonia water usually only takes a few seconds, as it's pH is usually high (pH 10). Tap water can have a pH of around 7, and may take 5-10 minutes to "blue". If the tap water is more acidic (pH 5 or below), the slides may not "blue". Now, we have to get all the nuclei from the reddish color to the bluish color. If the slide is not in the bluing agent for enough time (for the type/pH of bluing agent), then some of the nuclei change to blue, while some still remain reddish (or within a single nucleus, some of the DNA has changed to blue, some remains reddish), hence a more purple color. So, the easiest thing to try right now is to stain 2 slides from the same block (serial sections) in your hematoxylin for the same time, put one in the bluing agent for the usual time, put the other in the alkaline solution for extended time, and see if the nuclei on the second slide are now more blue than the first slide. Let us know. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: Webb, Dorothy L Sent: Friday, June 24, 2011 3:20 PM To: 'histonet <@t> lists.utsouthwestern.edu' Subject: [Histonet] bluing Looking to change my "bluing" step in the H&E process to obtain a bluer (less purple) hue to the nuclear detail. What is everyone using in their bluing step?? Thanks for all of your ideas!! From coralmani <@t> yahoo.co.in Sat Jun 25 12:36:06 2011 From: coralmani <@t> yahoo.co.in (mani kandan) Date: Sat Jun 25 12:36:11 2011 Subject: [Histonet] for phd offer Message-ID: <1309023366.93666.YahooMailClassic@web94702.mail.in2.yahoo.com> Hai histonetters, ????????????????? i am a master of science graduate working in stemcell research,looking for a phd offer or RA offer, currently i am working on cell derived from bone enosteal and central region cells. i am looking for research offer related to this field. i am looking for favourable reply. thank u. M.Manikandan, Researcher, Stemcell unit, King Saud university, Riyadh,KSA +966552012697 From Nancy_Schmitt <@t> pa-ucl.com Sat Jun 25 12:49:21 2011 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Sat Jun 25 12:49:27 2011 Subject: [Histonet] Leica Bond for IHCs In-Reply-To: <20110625170243.E9FD4142CD6@mail.pa-ucl.com> References: <20110625170243.E9FD4142CD6@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36790501@PEITHA.wad.pa-ucl.com> We are very happy with our BOND and technical support has been great for any and all questions/issues. Nancy Schmitt HT,MLT(ASCP) Dubuque, IA ------------------------------ Message: 16 Date: Sat, 25 Jun 2011 12:00:44 -0400 From: Sheila Adey Subject: [Histonet] Leica Bond for IHCs To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello netters: Looking for opinions on the Leica Bond immuno stainer please. Thanks. Sheila NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From amosbrooks <@t> gmail.com Sat Jun 25 13:19:01 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Jun 25 13:19:05 2011 Subject: [Histonet] Re: Histonet Digest, Vol 91, Issue 34 In-Reply-To: <4e061436.259bec0a.3199.4159SMTPIN_ADDED@mx.google.com> References: <4e061436.259bec0a.3199.4159SMTPIN_ADDED@mx.google.com> Message-ID: Hi, I prefer to use the plastic two slide mailers that lay the slides flat. The blade edge doesn't come in contact with anything that can mar it's sharpened surface like other blades or the edge of a container. I also mark the used blades with a sharpie so I can see which blade is totally spent for facing only, and which portion of the partially used blade is fresh. It also closes up safely so no one can accidentally get cut. Amos On Sat, Jun 25, 2011 at 1:00 PM, wrote: > Message: 7 > Date: Fri, 24 Jun 2011 15:53:39 -0500 > From: "Webb, Dorothy L" > Subject: [Histonet] blades > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > < > 65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD81@HPEMX3.HealthPartners.int> > > Content-Type: text/plain; charset="us-ascii" > > Trying to clean up some things hanging out there in our lab and wondering > what everyone does with a blade that has been used minimally and tech done > for the day with the microtome. Where do you store that blade for use > tomorrow or do you toss and not worry about the cost involved? I do not > like them sitting on top of the microtome. Any good ideas?? Thanks, as > always! > From joanne0658 <@t> comcast.net Sat Jun 25 16:50:14 2011 From: joanne0658 <@t> comcast.net (Joanne) Date: Sat Jun 25 16:49:38 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: AANLkTimSmJ6JO=uRvj_djA-3h5Q5gjf2p2fEEG5mjtnE@mail.gmail.com Message-ID: <498DDA84FDA449B284C69014FBF60684@JoannePC> i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) From Barry.R.Rittman <@t> uth.tmc.edu Sat Jun 25 17:27:05 2011 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Sat Jun 25 17:27:09 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: <498DDA84FDA449B284C69014FBF60684@JoannePC> References: , <498DDA84FDA449B284C69014FBF60684@JoannePC> Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E9018D864C95@UTHCMS1.uthouston.edu> Joanne I assume that you are not having us on as this seems a ridiculous goal to set. I would ask to look at previous personnel records to determine where the employer managed to find a robot that did this previously and ask your supervisor to show how he/she cuts one block per minute. It is obvious that the time taken to carry out cutting depends to a large extent on the challenge of the tissue blocks. It is also obvious that with such an attitude there will be less attention to quality and a greater likelihood of errors occurring.I would point out to management the potential lawsuits that might occur if a mistake is made and I suspect that they have not thought this through at all or are just trying to see how far they can go. An alternate solution would be for you to ask for a set sum per block and see their response. 53 is no age at all kid so hang in there and if you get an unfavorable response from management point out the big job market out there and the severe lack of skilled histotechs. My response to them would be more graphic. Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne [joanne0658@comcast.net] Sent: Saturday, June 25, 2011 4:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joanne0658 <@t> comcast.net Sat Jun 25 18:07:07 2011 From: joanne0658 <@t> comcast.net (Joanne) Date: Sat Jun 25 18:06:33 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: 498DDA84FDA449B284C69014FBF60684@JoannePC Message-ID: <9EB5BC908E6B44D88EB737A79778A3CD@JoannePC> i am quite serious in my presentation and request for advice. i too thought this goal was/is ridiculous to expect/ask for from someone so new and to attain in 6 months or less. last monday i embedded 214 blocks and cut 148 between 5am and 10:30am (we had almost 600 cassettes to share among 3 people) . . . .for someone so very new i thought this pretty good . . . please note: most days aren't as hectic. :) what is an average though for blocks/minute? what is meant by set sum per block? <---keeping in mind i am new to this field. From histotech <@t> imagesbyhopper.com Sat Jun 25 19:18:45 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat Jun 25 19:18:54 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: <12A4DAFC2FEBB84B8DED5F5E9201B4E9018D864C95@UTHCMS1.uthouston.edu> References: <498DDA84FDA449B284C69014FBF60684@JoannePC> <12A4DAFC2FEBB84B8DED5F5E9201B4E9018D864C95@UTHCMS1.uthouston.edu> Message-ID: <09B3D09F-DD6B-4878-8F9C-5D31485FF5B5@imagesbyhopper.com> Not to add fuel and all that... but I can, and regularly do, cut single slide blocks in less than minute per block. I am not a new tech though, been around the microtome a few times! I think the proper approach would be to have the tech *average* the number of slides the cut over a period of time. Then watch the trend, are you showing improvement over time? I think forward progress is what should be watched for, while maintaining quality. Quality is prarmount. Sent from my iPhone On Jun 25, 2011, at 6:27 PM, "Rittman, Barry R" wrote: > Joanne > I assume that you are not having us on as this seems a ridiculous goal to set. > I would ask to look at previous personnel records to determine where the employer managed to find a robot that did this previously and ask your supervisor to show how he/she cuts one block per minute. > It is obvious that the time taken to carry out cutting depends to a large extent on the challenge of the tissue blocks. > It is also obvious that with such an attitude there will be less attention to quality and a greater likelihood of errors occurring.I would point out to management the potential lawsuits that might occur if a mistake is made and I suspect that they have not thought this through at all or are just trying to see how far they can go. > An alternate solution would be for you to ask for a set sum per block and see their response. > 53 is no age at all kid so hang in there and if you get an unfavorable response from management point out the big job market out there and the severe lack of skilled histotechs. > My response to them would be more graphic. > Barry > > > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne [joanne0658@comcast.net] > Sent: Saturday, June 25, 2011 4:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] How many tissues an histo tech is suppose to cut per > > i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tjasper <@t> copc.net Sat Jun 25 20:01:53 2011 From: tjasper <@t> copc.net (Thomas Jasper) Date: Sat Jun 25 20:01:59 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per References: <498DDA84FDA449B284C69014FBF60684@JoannePC> Message-ID: <90354A475B420441B2A0396E5008D49692C037@copc-sbs.COPC.local> Joanne, In my humble opinion and without knowing anything about where you work, this expectation is ludicrous. I have a hard time believing that anyone, regardless of experience could attain the goal you've mentioned (including answering phones and running instruments). There are a multitude of reasons why this is absurd. Suffice it to say, I would be suspect of any work coming out of such an operation, not to mention those in charge and the poor souls trying to meet this goal. I seriously doubt you'll be able to have much say about things since you are so new. My advice...get out and find a job working for realistic people. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Sat Jun 25 20:22:59 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Sat Jun 25 20:23:09 2011 Subject: [Histonet] Leica Bond for IHCs In-Reply-To: References: Message-ID: greatest thing since sliced bread! ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sheila Adey [sadey@hotmail.ca] Sent: Saturday, June 25, 2011 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond for IHCs Hello netters: Looking for opinions on the Leica Bond immuno stainer please. Thanks. Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From tjasper <@t> copc.net Sat Jun 25 20:48:29 2011 From: tjasper <@t> copc.net (Thomas Jasper) Date: Sat Jun 25 20:48:35 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per References: <498DDA84FDA449B284C69014FBF60684@JoannePC> <90354A475B420441B2A0396E5008D49692C037@copc-sbs.COPC.local> Message-ID: <90354A475B420441B2A0396E5008D49692C038@copc-sbs.COPC.local> Well Joanne, someone on the Histonet probably has a documented average. It is a difficult thing due to the amount of variables that exist and the differences from lab to lab. Again, in my humble opinion (and not knowing anything about your workplace) to me, working in a mid-sized clinical service and dealing with the variety of specimens common to a lab such as ours...if someone is cutting 25-30 blocks per hour, the sections are high quality and the errors are negligible (a subjective statement) I would consider that more than acceptable. Others may be of a different opinion, however I would be surprised if someone would think that one block per minute is reasonable and realistic (except for your employers). I'm sorry to hear that it took you 10 months to secure employment at your current "Roman galley" ship of a service. There are jobs available and not enough of us to go around. I'd seriously consider breaking the leg iron, abandoning ship and taking your chances in the wider world. There are a lot of nice, sane people out there and I'm sure you could find a place that would appreciate and treat you fairly. tj -----Original Message----- From: Joanne [mailto:joanne0658@comcast.net] Sent: Saturday, June 25, 2011 6:31 PM To: Thomas Jasper Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per Tom, Thank you for your response. As it took me almost 10 months to secure a position, I'm not likely to be able to jump ship. Is there a documented average for a histotech's performance to be judged against? Again, thank you. Joanne ----- Original Message ----- From: "Thomas Jasper" To: "Joanne" Cc: Sent: Saturday, June 25, 2011 9:01 PM Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per Joanne, In my humble opinion and without knowing anything about where you work, this expectation is ludicrous. I have a hard time believing that anyone, regardless of experience could attain the goal you've mentioned (including answering phones and running instruments). There are a multitude of reasons why this is absurd. Suffice it to say, I would be suspect of any work coming out of such an operation, not to mention those in charge and the poor souls trying to meet this goal. I seriously doubt you'll be able to have much say about things since you are so new. My advice...get out and find a job working for realistic people. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Sat Jun 25 20:57:01 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Sat Jun 25 20:57:05 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: <90354A475B420441B2A0396E5008D49692C037@copc-sbs.COPC.local> References: <498DDA84FDA449B284C69014FBF60684@JoannePC> <90354A475B420441B2A0396E5008D49692C037@copc-sbs.COPC.local> Message-ID: Joanne - I have to agree with Tom and Barry in questioning how they have come to this time per block and the time frame with which they expect you to do this in. I was astonished at what I read in your second e-mail in terms of what you did on that one day in only six hours - do you still have all ten of your fingers!!!!! I'm not making fun - the pressure can be incredible when you first start in this field. I did a little internet searching and found some interesting information. 'Histology workload standards' brought up a lot of information and publications regarding standards for productivity. Rene Buesa had several publications that had good information and could give you a chance to compare your lab to what is out there in reasonable expections of productivity. The ASCP website didn't open up for me tonight (probably out for coffee), and I didn't go to NSH website which has other links. Use the internet to give yourself some information and then approach things from there. Hope this helps you some. I'm old school, learn the techniques first, the speed will fall into place and quality won't be at risk. Vikki On Sat, Jun 25, 2011 at 9:01 PM, Thomas Jasper wrote: > Joanne, > > In my humble opinion and without knowing anything about where you work, > this expectation is ludicrous. I have a hard time believing that > anyone, regardless of experience could attain the goal you've mentioned > (including answering phones and running instruments). There are a > multitude of reasons why this is absurd. Suffice it to say, I would be > suspect of any work coming out of such an operation, not to mention > those in charge and the poor souls trying to meet this goal. > > I seriously doubt you'll be able to have much say about things since you > are so new. My advice...get out and find a job working for realistic > people. > > Good luck, > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services > Bend, Oregon 97701 > 541/693-2677 > tjasper@copc.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne > Sent: Saturday, June 25, 2011 2:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] How many tissues an histo tech is suppose to cut per > > > > i've only been working 2 months. although older, i am new as a > histotech (graduated in may 2010, found a job in april 2011). seems > management is setting a goal of a block per minute as far as cutting > goes for me. i have until october to attain this goal. this minute for > cutting is to include facing, writing out slides, cutting, and putting > tray into symphony stainer (not to mention getting up to answer the > phone, fielding questions regarding send-out cases, and other slight > "cutting interruptions). this seems an extreme, possibly unattainable > goal. i'm up for a challenge at age 53, but any advice would be > SWONDERFUL!!!! :) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histotalk <@t> yahoo.com Sat Jun 25 21:32:57 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Sat Jun 25 21:33:03 2011 Subject: [Histonet] AL state meeting? In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B37043AD26C@giamail2.Gia.com> References: <03C921A1EAF7F541B16543F6EC6A4B37042B28FD@giamail2.Gia.com> <03C921A1EAF7F541B16543F6EC6A4B37043AD26C@giamail2.Gia.com> Message-ID: <1309055577.71616.YahooMailRC@web120603.mail.ne1.yahoo.com> Hi Amber - I was just doing some show prep for my show tomorrow night and checked my notes. I know I didn't mention any for AL so far this year and I don't have anything for them in the near future. I always mention the state meetings. Yours, Dave ________________________________ From: Amber McKenzie To: histonet@lists.utsouthwestern.edu Sent: Thu, June 23, 2011 4:36:54 PM Subject: [Histonet] AL state meeting? Does anyone know if there's an AL state meeting this year?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Sun Jun 26 00:14:07 2011 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Sun Jun 26 00:14:35 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: <9EB5BC908E6B44D88EB737A79778A3CD@JoannePC> References: 498DDA84FDA449B284C69014FBF60684@JoannePC, <9EB5BC908E6B44D88EB737A79778A3CD@JoannePC> Message-ID: I understand your frustration and think that the lab you work at, or any lab, that uses production standards MUST minimally explain how the standard is set and provide you a training and competency plan/schedule to attain the standard. If that has not been provided, I suggest you request a conversation with your Human Resources representative and/or the Lab Manager. You must have a clear understanding of the expectations set for you. That said, I do not understand why any lab would set the standard at microtomy by number of blocks cut, blocks should be used when setting the embedding task standard. I believe the correct unit of measure is the slide. My experience in the Histology lab is that every block can be different (some GI or Prostate labs are an exception) and even if they are not, the number of slides produced is a more accurate unit to count. My MAJOR concern is setting a production standard without coupling it with a QUALITY standard. Production standards mean nothing if the highest quality cannot be attained and maintained throughout the length of task performance. The development of Production and Quality Standards should be a reflection of the entire group of trained and competent employees performing the task. This includes ALL employees, new hire to highest level of competency. I also think it a mistake to set task Standards at a maximum level. Task standards should set as a minimum expectation level with encouragement and reward to perform above standard. The goal of developing Production/Quality Standards must always be employee improvement and reduction in defects/errors which will always lead to improved patient care. William DeSalvo, B.S., HTL(ASCP) > From: joanne0658@comcast.net > To: histonet@lists.utsouthwestern.edu > Date: Sat, 25 Jun 2011 19:07:07 -0400 > Subject: [Histonet] How many tissues an histo tech is suppose to cut per > > i am quite serious in my presentation and request for advice. i too thought this goal was/is ridiculous to expect/ask for from someone so new and to attain in 6 months or less. last monday i embedded 214 blocks and cut 148 between 5am and 10:30am (we had almost 600 cassettes to share among 3 people) . . . .for someone so very new i thought this pretty good . . . please note: most days aren't as hectic. :) what is an average though for blocks/minute? what is meant by set sum per block? <---keeping in mind i am new to this field. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Sun Jun 26 07:31:03 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sun Jun 26 07:31:09 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: References: <498DDA84FDA449B284C69014FBF60684@JoannePC> <90354A475B420441B2A0396E5008D49692C037@copc-sbs.COPC.local> Message-ID: <82CEFE68-52CA-40D1-94A7-77012EAFE3E7@imagesbyhopper.com> To ensure that I am understood correctly: I agree that the expectations for Joanne are unreasonable and unfair. I have read here some replies that more acurately explain what I thought better than *I* did! - Speed (while maintaining quality) will come over time. One *must* have quality or speed is useless. - monitoring the slide output can be utilized as a tool towards showing positive progress. This could simply be cutting one more block/slide per day. There will come a time when one more just cannot reasonably be done, but at least striving towards it gives something to shoot for. I recall a conversation I had with a coworker some years ago with regards to embedding: (her) you should be able to improve by one block per day. (me) Really? At that rate we will only need one person, as they will be able to do it all! Sent from my iPhone On Jun 25, 2011, at 9:57 PM, Victoria Baker wrote: > Joanne - > > I have to agree with Tom and Barry in questioning how they have come to this > time per block and the time frame with which they expect you to do this in. > I was astonished at what I read in your second e-mail in terms of what you > did on that one day in only six hours - do you still have all ten of your > fingers!!!!! I'm not making fun - the pressure can be incredible when you > first start in this field. > > I did a little internet searching and found some interesting information. > 'Histology workload standards' brought up a lot of information and > publications regarding standards for productivity. Rene Buesa had > several publications that had good information and could give you a chance > to compare your lab to what is out there in reasonable expections of > productivity. The ASCP website didn't open up for me tonight (probably out > for coffee), and I didn't go to NSH website which has other links. Use the > internet to give yourself some information and then approach things from > there. > > Hope this helps you some. I'm old school, learn the techniques first, the > speed will fall into place and quality won't be at risk. > > Vikki > > > > > On Sat, Jun 25, 2011 at 9:01 PM, Thomas Jasper wrote: > >> Joanne, >> >> In my humble opinion and without knowing anything about where you work, >> this expectation is ludicrous. I have a hard time believing that >> anyone, regardless of experience could attain the goal you've mentioned >> (including answering phones and running instruments). There are a >> multitude of reasons why this is absurd. Suffice it to say, I would be >> suspect of any work coming out of such an operation, not to mention >> those in charge and the poor souls trying to meet this goal. >> >> I seriously doubt you'll be able to have much say about things since you >> are so new. My advice...get out and find a job working for realistic >> people. >> >> Good luck, >> Tom Jasper >> >> Thomas Jasper HT (ASCP) BAS >> Histology Supervisor >> Central Oregon Regional Pathology Services >> Bend, Oregon 97701 >> 541/693-2677 >> tjasper@copc.net >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne >> Sent: Saturday, June 25, 2011 2:50 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] How many tissues an histo tech is suppose to cut per >> >> >> >> i've only been working 2 months. although older, i am new as a >> histotech (graduated in may 2010, found a job in april 2011). seems >> management is setting a goal of a block per minute as far as cutting >> goes for me. i have until october to attain this goal. this minute for >> cutting is to include facing, writing out slides, cutting, and putting >> tray into symphony stainer (not to mention getting up to answer the >> phone, fielding questions regarding send-out cases, and other slight >> "cutting interruptions). this seems an extreme, possibly unattainable >> goal. i'm up for a challenge at age 53, but any advice would be >> SWONDERFUL!!!! :) >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From HornHV <@t> archildrens.org Sun Jun 26 08:19:17 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Sun Jun 26 08:19:24 2011 Subject: [Histonet] Leica Bond for IHCs In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB7198B85AC74@EVS1.archildrens.org> We love ours and service has been excellent along with technical support. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Saturday, June 25, 2011 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond for IHCs Hello netters: Looking for opinions on the Leica Bond immuno stainer please. Thanks. Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From rjbuesa <@t> yahoo.com Sun Jun 26 09:22:31 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jun 26 09:22:35 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: <9EB5BC908E6B44D88EB737A79778A3CD@JoannePC> Message-ID: <110225.86399.qm@web65710.mail.ac4.yahoo.com> Embedding = 60 blocks/hour; cutting = 24 blocks/hour. These are averages and many HTs embed/cut far more?or far less.There are no "beginner's standards". Ren? J. --- On Sat, 6/25/11, Joanne wrote: From: Joanne Subject: [Histonet] How many tissues an histo tech is suppose to cut per To: histonet@lists.utsouthwestern.edu Date: Saturday, June 25, 2011, 7:07 PM i am quite serious in my presentation and request for advice.? i too thought this goal was/is ridiculous to expect/ask for from someone so new and to attain in 6 months or less.? last monday i embedded 214 blocks and cut 148 between 5am and 10:30am (we had almost 600 cassettes to share among 3 people) . . . .for someone so very new i thought this pretty good . . . please note: most days aren't as hectic.? :)? what is an average though for blocks/minute?? what is meant by set sum per block? <---keeping in mind i am new to this field. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Jun 26 09:42:59 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jun 26 09:43:03 2011 Subject: Fw: Re: [Histonet] How many tissues an histo tech is suppose to cut per Message-ID: <643218.30016.qm@web65705.mail.ac4.yahoo.com> --- Joanne: Read the attachment so you can have an idea about productivity ranges and averages in different tasks in the lab. If you embedded 214 blocks and cut 148? in 5.5 hours, using the averages I provided, embedding should have taken 3.5 hours and cutting 6.2 hours which means you worked 1.76 times FASTER than the expected average. Ren? J. --- On Sat, 6/25/11, Joanne wrote: From: Joanne Subject: [Histonet] How many tissues an histo tech is suppose to cut per To: histonet@lists.utsouthwestern.edu Date: Saturday, June 25, 2011, 7:07 PM i am quite serious in my presentation and request for advice.? i too thought this goal was/is ridiculous to expect/ask for from someone so new and to attain in 6 months or less.? last monday i embedded 214 blocks and cut 148 between 5am and 10:30am (we had almost 600 cassettes to share among 3 people) . . . .for someone so very new i thought this pretty good . . . please note: most days aren't as hectic.? :)? what is an average though for blocks/minute?? what is meant by set sum per block? <---keeping in mind i am new to this field. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmattingly90 <@t> gmail.com Sun Jun 26 11:38:28 2011 From: dmattingly90 <@t> gmail.com (Denise Mattingly) Date: Sun Jun 26 11:38:31 2011 Subject: [Histonet] Sakura auto TEC and the Leica cassette printer Message-ID: Any out there using an auto TEC embedder and the Leica cassette Printer . We are having trouble with the paraform cassettes tumbling out of the shoot and getting hung up . Sakura wont help because its a Leica printer and Leica wont help because its a Sakura cassette Anyone have any suggestions?? Other than have someone flick the cassette so it fall down the shoot properly ? Any help or suggestions greatly appreciated Denise Mattingly Riverside Hospital Columbus OH. 614-566-5679 From dunatrsd <@t> sbcglobal.net Sun Jun 26 19:50:21 2011 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Sun Jun 26 19:50:27 2011 Subject: [Histonet] Histogel Problem In-Reply-To: References: Message-ID: <537818.64667.qm@web83913.mail.sp1.yahoo.com> Amos, I have had the same problem in the past, and posted my issues on the Histonet, however no one was able to help me out. At one point I even exchanged Histogel with a colleage severl hundred miles away, thinking that maybe I had a bad lot of Histogel. She did not have a problem with my Histogel and neither did?I with her Histogel. The shrinking was arbitrary. At times all of them look great and other times more than half shrunk and looked brittle. Even RA/Thermo did not have an answer for me. I decided to do an experiment. To not bore everyone on the Histonet and explain all of my experimental steps, what it boiled down to is that you need a long processing program on the processor. We use a VIP processor as well, and the processing program is at least 12 hours long. NO MORE PROBLEMS. Since I started using this progrem, every single Histogel block has been perfect. Let me know if you need any further info or explanation. Dusko Trajkovic HT ASCP Pfizer Inc. La Jolla ? ________________________________ From: Amos Brooks To: histonet@lists.utsouthwestern.edu Sent: Fri, June 24, 2011 10:29:09 AM Subject: [Histonet] Histogel Problem Hi, ? I have a problem with some blocks that were prepared with Histogel. I was hoping someone else might have had a similar problem and figured it out. I took a photo of the blocks that were mads and put them in a Picassa album here: https://picasaweb.google.com/lh/photo/APO3HsIMa2_jPOEs3QRGUjhz3qi22FNPb2i5JJnBCAk?feat=directlink ? The long & short of it is that the blocs were prepared by the researcher for me to process. They are mouse kidneys. Now it is entirely possible for him to have goofed something up in preparing the Histogel blocks. I wasn't there when he did it, but when I looked at them before processing, they all looked fine. (Like the adjacent good one in the photo). When they were processed they were placed in the VIP right next to each other. When I went to embed them this morning all but one of the four looked fine. The one that didn't come out well looked like the Histogel had shrunk up and shriveled around the kidneys. I am sure this will be aweful to cut, and the researcher is going to have a bird over it since this happened with another project previously. I would like to have a decent explanation for him, so if anyone knows what might have happened and has suggestions I would love to hear it (yes vendors too are welcome to answer this of course!). ? By the way, this was processed on a rather short cycle of 15-20 min per station of graded ETOH from 70% to 100% with 3 xylene stations and 4 paraffin stations (45 min for these). It seems fine for everything else that was on the processor. Just that one Histogel block was the issue. Thank you, Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vivek.Saroha <@t> nottingham.ac.uk Mon Jun 27 05:57:46 2011 From: Vivek.Saroha <@t> nottingham.ac.uk (Vivek Saroha) Date: Mon Jun 27 06:41:03 2011 Subject: [Histonet] Macrophage marker in sheep for IHC In-Reply-To: <4BFB9735C69E4BFFAB66E0ACC0EA1982@auxs.umn.edu> References: <834C946D8CED2340B4A9DC8401B55CE0057D03E826@EXCHANGE1.ad.nottingham.ac.uk> <4BFB9735C69E4BFFAB66E0ACC0EA1982@auxs.umn.edu> Message-ID: <834C946D8CED2340B4A9DC8401B55CE0057D03ECAA@EXCHANGE1.ad.nottingham.ac.uk> Thank you to everyone who has replied and given suggestions. I now have some ideas to help me start and will update and acknowledge once again once I make some progress. Best regards Vivek -----Original Message----- From: Jan Shivers [mailto:shive003@umn.edu] Sent: 24 June 2011 14:37 To: Vivek Saroha; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Macrophage marker in sheep for IHC CD68 - clone KP1 has not worked on sheep (also not dogs, cows, horses, and chickens) in my experience. I have found it to stain primates well (and rare cells in pigs and cats). Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ----- Original Message ----- From: "Vivek Saroha" To: Sent: Friday, June 24, 2011 4:05 AM Subject: [Histonet] Macrophage marker in sheep for IHC Hi there, I am very new to basic sciences research and am planning an experiment to quantify and compare adipose tissue macrophage infiltration in sheep model using immunohistochemistry. I am specifically interested in CD11c as a marker of M1 activation of Macrophages. I have been advised that it is not easy and previous effort by my predecessors have not been very successful in this! >From the general google, histonet/histosearch and pubmed search. I have >made a list of potential candidates: 1. Clones PG M1 and Clone KP1 (Source : Histonet : http://www.histosearch.com/histonet/Nov03A/RE.HistonetSeepmacrophage.html) 2. EMB11, a mouse anti-human CD68 mAb (Dako, Carpenteria, CA) (source: published paper: http://www.ncbi.nlm.nih.gov/pubmed/16460804 ) 1. Another CD68 antibody Source: http://www.ingentaconnect.com/content/ocean/ajra/2011/00000025/00000002/art00024) 1. Iba1 (ionized calcium binding adaptor molecule 1) is a 17-kDa EF hand protein that is specifically expressed in macrophages/microglia and is upregulated during the activation of these cells.[Wikipedia] (source: I do not remember where I found this one from but Abcam has several antibodies none of which are predicted for sheep) I am writing to ask for suggestions about which one to go for and any other tips about searching for antibodies or performing IHC in paraffin fixed adipose tissue/liver. Any ideas will be gratefully appreciated as I am very new and isolated in this aspect. Best wishes Vivek Dr Vivek Saroha Clinical lecturer in Child health University of Nottingham This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Mon Jun 27 09:27:53 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Mon Jun 27 09:27:57 2011 Subject: [Histonet] Tissue Shriveling in Paraffin In-Reply-To: <4E044A71.5876.00D9.0@auburn.edu> References: <4E044A71.5876.00D9.0@auburn.edu> Message-ID: You could have got the tissue wet or to hydrated. Sometimes after you face the block if you leave it on the ice to long and let moisture get into the tissue then cut the slide, it looks ok at the time, but once the moisture gets back out of the tissue it will start to look concave. If you are going to be leaving your blocks on the ice for a long time just put a 4X4 or paper towel under the block, this helps. Good luck =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Aono Sent: Friday, June 24, 2011 8:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Shriveling in Paraffin I was cutting some bone/joint tissue and noticed that the cartilaginous portion was concave/indented, instead of flush with the rest of the block surface. Even as I continued to cut that portion always seemed a little sunken into the block face and all the sections crumbled. I didn't seal the block after I was done and when I came back the next day the entire tissue sample was shriveled and pulling away from the paraffin. I'm new, but in the few bone sections I've done I've never had this happen! Any ideas? ~Shelly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Mon Jun 27 09:29:34 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Mon Jun 27 09:29:38 2011 Subject: [Histonet] RE: HistoGel In-Reply-To: <3FEFF18FF4E1914A9AB7D8498591BE8610CF058850@VEXCCR02.phsabc.ehcnet.ca> References: <82fc8dae-dfdf-46ae-bfaa-a86d4092b2f0@SRVEXHT01.phsabc.ehcnet.ca> <3FEFF18FF4E1914A9AB7D8498591BE8610CF058850@VEXCCR02.phsabc.ehcnet.ca> Message-ID: You can also use agar. It does the same thing and is cheaper =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Milne, Katy Sent: Friday, June 24, 2011 12:27 PM To: 'histonet@lists.utsouthwestern.edu'; 'mjdessoye@wvhcs.org' Subject: [Histonet] RE: HistoGel We use histogel a lot in our lab. It's a research lab and we use it for a few purposes - pelleting cultured cells then creating multi-culture TMAs for testing antibodies and also pelleting cells from ascites and pleural effusions. Has also been used to process really small samples that could have been lost in the processor through the cassettes. Works quite well. The researchers just put the samples in histogel and give it to me in formalin then I process it as I would regular tissue. Cuts very well too. Katy Message: 3 Date: Fri, 24 Jun 2011 12:26:46 -0400 From: "Dessoye, Michael J" Subject: [Histonet] HistoGel To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello, Does anyone out there have any experience with HistoGel? It's Richard Allan/Thermo Fisher. They claim that you can "embed" scant tissues in the gel and then process, embed, and cut as usual. Just wondering how it works in the real world.... Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Mon Jun 27 09:30:49 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Mon Jun 27 09:30:53 2011 Subject: [Histonet] bluing In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD7D@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD7D@HPEMX3.HealthPartners.int> Message-ID: Just add a little bit more ammonia hydroxide to the water =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, June 24, 2011 2:21 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bluing Looking to change my "bluing" step in the H&E process to obtain a bluer (less purple) hue to the nuclear detail. What is everyone using in their bluing step?? Thanks for all of your ideas!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Mon Jun 27 09:38:13 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Mon Jun 27 09:38:16 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cutper In-Reply-To: <643218.30016.qm@web65705.mail.ac4.yahoo.com> References: <643218.30016.qm@web65705.mail.ac4.yahoo.com> Message-ID: Another solution...get out of clinical and go into research =) There are no quotas or slide per second expectations in the research world =) So sorry you are having such a bad time with your job. HT's are not a dime a dozen and usually it is fairly easy to find a better job. Not to mention research pays almost double the clinical world!! Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, June 26, 2011 9:43 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: Fw: Re: [Histonet] How many tissues an histo tech is suppose to cutper --- Joanne: Read the attachment so you can have an idea about productivity ranges and averages in different tasks in the lab. If you embedded 214 blocks and cut 148? in 5.5 hours, using the averages I provided, embedding should have taken 3.5 hours and cutting 6.2 hours which means you worked 1.76 times FASTER than the expected average. Ren? J. --- On Sat, 6/25/11, Joanne wrote: From: Joanne Subject: [Histonet] How many tissues an histo tech is suppose to cut per To: histonet@lists.utsouthwestern.edu Date: Saturday, June 25, 2011, 7:07 PM i am quite serious in my presentation and request for advice.? i too thought this goal was/is ridiculous to expect/ask for from someone so new and to attain in 6 months or less.? last monday i embedded 214 blocks and cut 148 between 5am and 10:30am (we had almost 600 cassettes to share among 3 people) . . . .for someone so very new i thought this pretty good . . . please note: most days aren't as hectic.? :)? what is an average though for blocks/minute?? what is meant by set sum per block? <---keeping in mind i am new to this field. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marcia_Gaiser <@t> ssmhc.com Mon Jun 27 09:56:44 2011 From: Marcia_Gaiser <@t> ssmhc.com (Gaiser, Marcia) Date: Mon Jun 27 09:56:51 2011 Subject: [Histonet] Full Time HT position in Oklahoma City Ok Message-ID: <728F817C02110E498D803A7C3B0C6248068D43B59E@S009-APEXM06.ds.ad.ssmhc.com> POSITION REQUIREMENTS: EDUCATION: High school diploma or GED preferred. CERTIFICATION, LICENSURE, BONDING: Certified as an HT or HLT by the American Society of Clinical Pathologists (ASCP) ? or ? other nationally recognized certifying agency acceptable to the Laboratory Director ? or ? experience acceptable to the Laboratory Director. EXPERIENCE: Two years of satisfactory histology experience. SIGN ON BONUS AVAILABLE FOR QUALIFIED CANDIDATES. Outstanding benefits package including generous paid time off. Apply online at www.saintsok.com, Ad# 10762, or contact Anna King at (405) 272-6105 for more information. Thank you! Anna King HR Recruiter St. Anthony Hospital (405) 272-6105 - phone (405) 272-6781 - fax [https://mobile.ssmhc.com/owa/attachment.ashx?id=RgAAAABMsXdcX9YUQLcxZSN9Shu5BwByj4F8AhEOSY2AOnw7DGJIAAUpEpPHAAByj4F8AhEOSY2AOnw7DGJIAAaNIGfxAAAJ&attcnt=1&attid0=EACRQxAfu%2f2MRrpel0TNLrK5] Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From gagnone <@t> KGH.KARI.NET Mon Jun 27 10:02:29 2011 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Mon Jun 27 10:02:36 2011 Subject: [Histonet] How many tissues... Message-ID: To add to the recent discussion about how many blocks can be cut per hour, the College of Medical Laboratory Technologists of Ontario published Practice Guidelines for Medical Laboratory Technologists Practising in Histology fairly recently, in 2008, which may be of use in this context. The practice guidelines are "intended to support, not replace, the exercise of professional judgment by medical laboratory technologists practising in histology...and are maintained...in consultation with CMLTO members and stakeholders." The usual workplace variables are taken into account in the guideline, but at least there are some daily ranges presented which may help Joanne in her quest for a reasonable goal. Link follows: http://tinyurl.com/histology-guidelines Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario From madary <@t> verizon.net Mon Jun 27 10:04:21 2011 From: madary <@t> verizon.net (madary@verizon.net) Date: Mon Jun 27 10:04:29 2011 Subject: [Histonet] One block per minute Message-ID: <1050016837.2194995.1309187061970.JavaMail.root@vznit170072> It seems like this argument has been going on as long as the histonet = has been here for us. Just because some folks in histoland can cut 60 block= s per minute does not mean that should be the minimum standard, esp for a n= ew tech, come on that is just dangerous to make someone that nervous. = When we purchase a car that states it gets 23 mpg city and 31 mpg on the hi= ghway, those are in perfect conditions when all the stars align, going down= hill in neutral. These so called intutive managers who expect someone= to cut 60 blocks per hour clearly never had a pack of blades that took thr= ee minutes to get a blade to slide out, never had a dull blade, no interrup= tions, no fire alarms,no call for levels, decals, only had perfect processi= ng, perfect soak etc. In 30 plus years I have heard so many people tell me = they can do this, but have never witnessed such an incredible feat, day in = day out. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC Jun 26, 2011 12:01:08 PM, = histonet@lists.utsouthwestern.edu wrote: Send Histonet= mailing list submissions to histonet@lists.utsouthwestern.edu To= subscribe or unsubscribe via the World Wide Web, visit http://lists.uts= outhwestern.edu/mailman/listinfo/histonet or, via email, send a message = with subject or body 'help' to histonet-request@lists.utsouthwestern.edu= You can reach the person managing the list at histonet-owner@lis= ts.utsouthwestern.edu When replying, please edit your Subject line s= o it is more specific than "Re: Contents of Histonet digest..." <= BR>Today's Topics: 1. for phd offer (mani kandan) 2. Leica Bond f= or IHCs (Nancy Schmitt) 3. Re: Histonet Digest, Vol 91, Issue 34 (Amos B= rooks) 4. How many tissues an histo tech is suppose to cut per (Joanne)<= BR>5. RE: How many tissues an histo tech is suppose to cut per (Rittman,= Barry R) 6. How many tissues an histo tech is suppose to cut per (Joann= e) 7. Re: How many tissues an histo tech is suppose to cut per (histo= tech@imagesbyhopper.com) 8. RE: How many tissues an histo tech is suppos= e to cut per (Thomas Jasper) 9. RE: Leica Bond for IHCs (Houston, Ron= ald) 10. RE: How many tissues an histo tech is suppose to cut per (Th= omas Jasper) 11. Re: How many tissues an histo tech is suppose to cut pe= r (Victoria Baker) 12. Re: AL state meeting? (David Kemler) 13. RE= : How many tissues an histo tech is suppose to cut per (WILLIAM DESALVO)= 14. Re: How many tissues an histo tech is suppose to cut per (histot= ech@imagesbyhopper.com) 15. RE: Leica Bond for IHCs (Horn, Hazel V) 1= 6. Re: How many tissues an histo tech is suppose to cut per (Rene J Bues= a) 17. Fw: Re: [Histonet] How many tissues an histo tech is suppose t= o cut per (Rene J Buesa) 18. Sakura auto TEC and the Leica cassette prin= ter (Denise Mattingly) -----------------------------------------= ----------------------------- Message: 1 Date: Sat, 25 Jun 2011 2= 3:06:06 +0530 (IST) From: mani kandan Subject= : [Histonet] for phd offer To: histonet@lists.utsouthwestern.edu Mess= age-ID: <1309023366.93666.YahooMailClassic@web94702.mail.in2.yahoo.co= m> Content-Type: text/plain; charset=3Diso-8859-1 Hai hist= onetters, &n= bsp; i am a master of science graduate = working in stemcell research,looking for a phd offer or RA offer, currently= i am working on cell derived from bone enosteal and central region cells. = i am looking for research offer related to this field. i am looking for fav= ourable reply. thank u. M.Manikandan, Researcher, Stemcell uni= t, King Saud university, Riyadh,KSA +966552012697 ---------= --------------------- Message: 2 Date: Sat, 25 Jun 2011 17:49:21 = +0000 From: Nancy Schmitt Subject: [Histon= et] Leica Bond for IHCs To: "histonet@lists.utsouthwestern.edu" Message-ID: <906B4DA90ED1DB4DB6C7E9= 4D7CEE6C36790501@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; cha= rset=3D"us-ascii" We are very happy with our BOND and technical supp= ort has been great for any and all questions/issues. Nancy Schmitt H= T,MLT(ASCP) Dubuque, IA ------------------------------ Message= : 16 Date: Sat, 25 Jun 2011 12:00:44 -0400 From: Sheila Adey Subject: [Histonet] Leica Bond for IHCs To: Message-ID: Content-Type: text/plain; charset=3D"iso-8859-1" Hello= netters: Looking for opinions on the Leica Bond immuno stainer please. = Thanks. Sheila NOTICE: This email may contain legal= ly privileged information. The information is for the use of only the in= tended recipient(s) even if addressed incorrectly. If you are not the in= tended recipient, please notify the sender that you have received it in = error and then delete it along with any attachments. Thank you. <= BR> ------------------------------ Message: 3 Date: Sat, 2= 5 Jun 2011 14:19:01 -0400 From: Amos Brooks Su= bject: [Histonet] Re: Histonet Digest, Vol 91, Issue 34 To: histonet@lis= ts.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=3DISO-8859-1 Hi,= I prefer to use the plastic two slide mailers that lay the slides flat.= The blade edge doesn't come in contact with anything that can mar it's<= BR>sharpened surface like other blades or the edge of a container. I also m= ark the used blades with a sharpie so I can see which blade is totally s= pent for facing only, and which portion of the partially used blade is f= resh. It also closes up safely so no one can accidentally get cut. Amos On Sat, Jun 25, 2011 at 1:00 PM, wrote: > Message: 7 > Date: Fri, 24 Jun 2= 011 15:53:39 -0500 > From: "Webb, Dorothy L" > Subject: [Histonet] blades > To: "'histonet@lists= .utsouthwestern.edu'" > > M= essage-ID: > < > 65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD8= 1@HPEMX3.HealthPartners.int> > > Content-Type: text/plain; c= harset=3D"us-ascii" > > Trying to clean up some things hanging = out there in our lab and wondering > what everyone does with a blade = that has been used minimally and tech done > for the day with the mic= rotome. Where do you store that blade for use > tomorrow or do you to= ss and not worry about the cost involved? I do not > like them sittin= g on top of the microtome. Any good ideas?? Thanks, as > always! &= gt; ------------------------------ Message: 4 Date: Sa= t, 25 Jun 2011 17:50:14 -0400 From: "Joanne" Subject: [Histonet] How many tissues an histo tech is suppose to cut pe= r To: Message-ID: <498DDA84FDA= 449B284C69014FBF60684@JoannePC> Content-Type: text/plain; charset=3D"= iso-8859-1" i've only been working 2 months. although older,= i am new as a histotech (graduated in may 2010, found a job in april 2011)= . seems management is setting a goal of a block per minute as far as cuttin= g goes for me. i have until october to attain this goal. this minute for cu= tting is to include facing, writing out slides, cutting, and putting tray i= nto symphony stainer (not to mention getting up to answer the phone, fieldi= ng questions regarding send-out cases, and other slight "cutting interrupti= ons). this seems an extreme, possibly unattainable goal. i'm up for a chall= enge at age 53, but any advice would be SWONDERFUL!!!! :) -= ----------------------------- Message: 5 Date: Sat, 25 Jun 2011 1= 7:27:05 -0500 From: "Rittman, Barry R" = Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut= per To: "histonet@lists.utsouthwestern.edu" Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E9018D864C9= 5@UTHCMS1.uthouston.edu> Content-Type: text/plain; charset=3D"us-asci= i" Joanne I assume that you are not having us on as this seems a = ridiculous goal to set. I would ask to look at previous personnel record= s to determine where the employer managed to find a robot that did this pre= viously and ask your supervisor to show how he/she cuts one block per minut= e. It is obvious that the time taken to carry out cutting depends to a l= arge extent on the challenge of the tissue blocks. It is also obvious th= at with such an attitude there will be less attention to quality and a grea= ter likelihood of errors occurring.I would point out to management the pote= ntial lawsuits that might occur if a mistake is made and I suspect that the= y have not thought this through at all or are just trying to see how far th= ey can go. An alternate solution would be for you to ask for a set sum p= er block and see their response. 53 is no age at all kid so hang in ther= e and if you get an unfavorable response from management point out the big = job market out there and the severe lack of skilled histotechs. My respo= nse to them would be more graphic. Barry ________________= ________________________ From: histonet-bounces@lists.utsouthwestern.edu= [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne [joanne065= 8@comcast.net] Sent: Saturday, June 25, 2011 4:50 PM To: histonet@lis= ts.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech= is suppose to cut per i've only been working 2 months. although old= er, i am new as a histotech (graduated in may 2010, found a job in april 20= 11). seems management is setting a goal of a block per minute as far as cut= ting goes for me. i have until october to attain this goal. this minute for= cutting is to include facing, writing out slides, cutting, and putting tra= y into symphony stainer (not to mention getting up to answer the phone, fie= lding questions regarding send-out cases, and other slight "cutting interru= ptions). this seems an extreme, possibly unattainable goal. i'm up for a ch= allenge at age 53, but any advice would be SWONDERFUL!!!! :) _______= ________________________________________ Histonet mailing list Histon= et@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/list= info/histonet ------------------------------ Message: 6Date: Sat, 25 Jun 2011 19:07:07 -0400 From: "Joanne" Subject: [Histonet] How many tissues an histo tech is suppose to= cut per To: Message-ID: <9= EB5BC908E6B44D88EB737A79778A3CD@JoannePC> Content-Type: text/plain; c= harset=3D"iso-8859-1" i am quite serious in my presentation and requ= est for advice. i too thought this goal was/is ridiculous to expect/ask for= from someone so new and to attain in 6 months or less. last monday i embed= ded 214 blocks and cut 148 between 5am and 10:30am (we had almost 600 casse= ttes to share among 3 people) . . . .for someone so very new i thought this= pretty good . . . please note: most days aren't as hectic. :) what is an a= verage though for blocks/minute? what is meant by set sum per block? <--= -keeping in mind i am new to this field. -----------= ------------------- Message: 7 Date: Sat, 25 Jun 2011 20:18:45 -0= 400 From: "histotech@imagesbyhopper.com" <= BR>Subject: Re: [Histonet] How many tissues an histo tech is suppose to = cut per To: "Rittman, Barry R" Cc: "his= tonet@lists.utsouthwestern.edu" M= essage-ID: <09B3D09F-DD6B-4878-8F9C-5D31485FF5B5@imagesbyhopper.com><= BR>Content-Type: text/plain; charset=3Dus-ascii Not to add fuel and = all that... but I can, and regularly do, cut single slide blocks in less th= an minute per block. I am not a new tech though, been around the microtome = a few times! I think the proper approach would be to have the tech *= average* the number of slides the cut over a period of time. Then watch the= trend, are you showing improvement over time? I think forward progress is = what should be watched for, while maintaining quality. Quality is prarmount= . Sent from my iPhone On Jun 25, 2011, at 6:27 PM, "Rittman, = Barry R" wrote: > Joanne > I a= ssume that you are not having us on as this seems a ridiculous goal to set.= > I would ask to look at previous personnel records to determine whe= re the employer managed to find a robot that did this previously and ask yo= ur supervisor to show how he/she cuts one block per minute. > It is o= bvious that the time taken to carry out cutting depends to a large extent o= n the challenge of the tissue blocks. > It is also obvious that with = such an attitude there will be less attention to quality and a greater like= lihood of errors occurring.I would point out to management the potential la= wsuits that might occur if a mistake is made and I suspect that they have n= ot thought this through at all or are just trying to see how far they can g= o. > An alternate solution would be for you to ask for a set sum per = block and see their response. > 53 is no age at all kid so hang in th= ere and if you get an unfavorable response from management point out the bi= g job market out there and the severe lack of skilled histotechs. > M= y response to them would be more graphic. > Barry > > > > ________________________________________ > From: hist= onet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwester= n.edu] On Behalf Of Joanne [joanne0658@comcast.net] > Sent: Saturday,= June 25, 2011 4:50 PM > To: histonet@lists.utsouthwestern.edu >= ; Subject: [Histonet] How many tissues an histo tech is suppose to cut per<= BR>> > i've only been working 2 months. although older, i am new = as a histotech (graduated in may 2010, found a job in april 2011). seems ma= nagement is setting a goal of a block per minute as far as cutting goes for= me. i have until october to attain this goal. this minute for cutting is t= o include facing, writing out slides, cutting, and putting tray into sympho= ny stainer (not to mention getting up to answer the phone, fielding questio= ns regarding send-out cases, and other slight "cutting interruptions). this= seems an extreme, possibly unattainable goal. i'm up for a challenge at ag= e 53, but any advice would be SWONDERFUL!!!! :) > > __________= _____________________________________ > Histonet mailing list >= Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/= mailman/listinfo/histonet > _________________________________________= ______ > Histonet mailing list > Histonet@lists.utsouthwestern.= edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet &g= t; ------------------------------ Message: 8 Date= : Sat, 25 Jun 2011 18:01:53 -0700 From: "Thomas Jasper" Subject: RE: [Histonet] How many tissues an histo tech is suppose to<= BR>cut per To: "Joanne" Cc: histonet@lists.u= tsouthwestern.edu Message-ID: <90354A475B420441B2A0396E5008D49692C= 037@copc-sbs.COPC.local> Content-Type: text/plain; charset=3D"us-asci= i" Joanne, In my humble opinion and without knowing anything = about where you work, this expectation is ludicrous. I have a hard time = believing that anyone, regardless of experience could attain the goal yo= u've mentioned (including answering phones and running instruments). The= re are a multitude of reasons why this is absurd. Suffice it to say, I w= ould be suspect of any work coming out of such an operation, not to ment= ion those in charge and the poor souls trying to meet this goal. = I seriously doubt you'll be able to have much say about things since you are so new. My advice...get out and find a job working for realistic pe= ople. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend= , Oregon 97701 541/693-2677 tjasper@copc.net -----Original Mes= sage----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:his= tonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturd= ay, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subje= ct: [Histonet] How many tissues an histo tech is suppose to cut per = i've only been working 2 months. although older, i am new as a h= istotech (graduated in may 2010, found a job in april 2011). seems manag= ement is setting a goal of a block per minute as far as cutting goes for= me. i have until october to attain this goal. this minute for cutting i= s to include facing, writing out slides, cutting, and putting tray into = symphony stainer (not to mention getting up to answer the phone, fieldin= g questions regarding send-out cases, and other slight "cutting interrup= tions). this seems an extreme, possibly unattainable goal. i'm up for a = challenge at age 53, but any advice would be SWONDERFUL!!!! :) _= ______________________________________________ Histonet mailing list = Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailma= n/listinfo/histonet ------------------------------ Message: 9 Date: Sun, 26 Jun 2011 01:22:59 +0000 From: "Houston= , Ronald" Subject: RE: [Histone= t] Leica Bond for IHCs To: Sheila Adey , "histonet@= lists.utsouthwestern.edu" Message= -ID: Content-Type: text/plain; charset=3D"us-ascii" greatest = thing since sliced bread! ________________________________________From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.u= tsouthwestern.edu] on behalf of Sheila Adey [sadey@hotmail.ca] Sent: Sat= urday, June 25, 2011 12:00 PM To: histonet@lists.utsouthwestern.edu S= ubject: [Histonet] Leica Bond for IHCs Hello netters: Looking for= opinions on the Leica Bond immuno stainer please. Thanks. Sheila ___= ____________________________________________ Histonet mailing list Hi= stonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/= listinfo/histonet ----------------------------------------- Confidential= ity Notice: The following mail message, including any attachments, is fo= r the sole use of the intended recipient(s) and may contain confidential= and privileged information. The recipient is responsible to maintain= the confidentiality of this information and to use the information only= for authorized purposes. If you are not the intended recipient (or auth= orized to receive information for the intended recipient), you are hereb= y notified that any review, use, disclosure, distribution, copying, prin= ting, or action taken in reliance on the contents of this e-mail is stri= ctly prohibited. If you have received this communication in error, pleas= e notify us immediately by reply e-mail and destroy all copies of the or= iginal message. Thank you. ------------------------------= Message: 10 Date: Sat, 25 Jun 2011 18:48:29 -0700 From: "Thom= as Jasper" Subject: RE: [Histonet] How many tissues a= n histo tech is suppose to cut per To: "Joanne" Cc: histonet@lists.utsouthwestern.edu Message-ID: <90354A47= 5B420441B2A0396E5008D49692C038@copc-sbs.COPC.local> Content-Type: tex= t/plain; charset=3D"us-ascii" Well Joanne, someone on the Histonet p= robably has a documented average. It is a difficult thing due to the amo= unt of variables that exist and the differences from lab to lab. Again, = in my humble opinion (and not knowing anything about your workplace) to = me, working in a mid-sized clinical service and dealing with the variety= of specimens common to a lab such as ours...if someone is cutting 25-30= blocks per hour, the sections are high quality and the errors are negli= gible (a subjective statement) I would consider that more than acceptabl= e. Others may be of a different opinion, however I would be surprised if= someone would think that one block per minute is reasonable and realist= ic (except for your employers). I'm sorry to hear that it took y= ou 10 months to secure employment at your current "Roman galley" ship of= a service. There are jobs available and not enough of us to go around. = I'd seriously consider breaking the leg iron, abandoning ship and taking= your chances in the wider world. There are a lot of nice, sane people o= ut there and I'm sure you could find a place that would appreciate and t= reat you fairly. tj -----Original Message----- From: Joanne [m= ailto:joanne0658@comcast.net] Sent: Saturday, June 25, 2011 6:31 PM = To: Thomas Jasper Subject: Re: [Histonet] How many tissues an histo tech= is suppose to cut per Tom, Thank you for your response. A= s it took me almost 10 months to secure a position, I'm not likely t= o be able to jump ship. Is there a documented average for a histotec= h's performance to be judged against? Again, thank you. Joanne ----- Original Message ----- From: "Thomas Jasper" To: "Joanne" Cc: Sent: Saturday, June 25, 2011 9:01 PM Subject: RE= : [Histonet] How many tissues an histo tech is suppose to cut per Joanne, In my humble opinion and without knowing anything about= where you work, this expectation is ludicrous. I have a hard time belie= ving that anyone, regardless of experience could attain the goal you've = mentioned (including answering phones and running instruments). There ar= e a multitude of reasons why this is absurd. Suffice it to say, I would = be suspect of any work coming out of such an operation, not to mentionthose in charge and the poor souls trying to meet this goal. I ser= iously doubt you'll be able to have much say about things since you are = so new. My advice...get out and find a job working for realistic people.= Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS Hist= ology Supervisor Central Oregon Regional Pathology Services Bend, Ore= gon 97701 541/693-2677 tjasper@copc.net -----Original Message-= ---- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet= -bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, J= une 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [= Histonet] How many tissues an histo tech is suppose to cut per <= BR>i've only been working 2 months. although older, i am new as a histot= ech (graduated in may 2010, found a job in april 2011). seems management= is setting a goal of a block per minute as far as cutting goes for me. = i have until october to attain this goal. this minute for cutting is to = include facing, writing out slides, cutting, and putting tray into symph= ony stainer (not to mention getting up to answer the phone, fielding que= stions regarding send-out cases, and other slight "cutting interruptions= ). this seems an extreme, possibly unattainable goal. i'm up for a chall= enge at age 53, but any advice would be SWONDERFUL!!!! :) _______= ________________________________________ Histonet mailing list Histon= et@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/list= info/histonet --------------------------= ---- Message: 11 Date: Sat, 25 Jun 2011 21:57:01 -0400 From: V= ictoria Baker Subject: Re: [Histonet] How many = tissues an histo tech is suppose to cut per To: Thomas Jasper Cc: histonet@lists.utsouthwestern.edu, Joanne Message-ID: Content-Type: text/plain; charset=3DISO-8859-1 Joanne - = I have to agree with Tom and Barry in questioning how they have come to= this time per block and the time frame with which they expect you to do= this in. I was astonished at what I read in your second e-mail in terms= of what you did on that one day in only six hours - do you still have a= ll ten of your fingers!!!!! I'm not making fun - the pressure can be inc= redible when you first start in this field. I did a little intern= et searching and found some interesting information. 'Histology workload= standards' brought up a lot of information and publications regarding s= tandards for productivity. Rene Buesa had several publications that had = good information and could give you a chance to compare your lab to what= is out there in reasonable expections of productivity. The ASCP website= didn't open up for me tonight (probably out for coffee), and I didn't g= o to NSH website which has other links. Use the internet to give yoursel= f some information and then approach things from there. Hope this= helps you some. I'm old school, learn the techniques first, the speed w= ill fall into place and quality won't be at risk. Vikki <= BR> On Sat, Jun 25, 2011 at 9:01 PM, Thomas Jasper wro= te: > Joanne, > > In my humble opinion and without kn= owing anything about where you work, > this expectation is ludicrous.= I have a hard time believing that > anyone, regardless of experience= could attain the goal you've mentioned > (including answering phones= and running instruments). There are a > multitude of reasons why thi= s is absurd. Suffice it to say, I would be > suspect of any work comi= ng out of such an operation, not to mention > those in charge and the= poor souls trying to meet this goal. > > I seriously doubt you= 'll be able to have much say about things since you > are so new. My = advice...get out and find a job working for realistic > people. &g= t; > Good luck, > Tom Jasper > > Thomas Jasper HT (= ASCP) BAS > Histology Supervisor > Central Oregon Regional Path= ology Services > Bend, Oregon 97701 > 541/693-2677 > tjas= per@copc.net > > -----Original Message----- > From: histo= net-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists= .utsouthwestern.edu] On Behalf Of Joanne > Sent: Saturday, June 25, 2= 011 2:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: = [Histonet] How many tissues an histo tech is suppose to cut per > = > > > i've only been working 2 months. although older, i am = new as a > histotech (graduated in may 2010, found a job in april 201= 1). seems > management is setting a goal of a block per minute as far= as cutting > goes for me. i have until october to attain this goal. = this minute for > cutting is to include facing, writing out slides, c= utting, and putting > tray into symphony stainer (not to mention gett= ing up to answer the > phone, fielding questions regarding send-out c= ases, and other slight > "cutting interruptions). this seems an extre= me, possibly unattainable > goal. i'm up for a challenge at age 53, b= ut any advice would be > SWONDERFUL!!!! :) > > __________= _____________________________________ > Histonet mailing list >= Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/= mailman/listinfo/histonet > > > > _________________= ______________________________ > Histonet mailing list > Histon= et@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman= /listinfo/histonet > ------------------------------ Message: 12 Date: Sat, 25 Jun 2011 19:32:57 -0700 (PDT) From: David = Kemler Subject: Re: [Histonet] AL state meeting?To: Fellow HistoNetters Message-ID= : <1309055577.71616.YahooMailRC@web120603.mail.ne1.yahoo.com> C= ontent-Type: text/plain; charset=3Diso-8859-1 Hi Amber - I wa= s just doing some show prep for my show tomorrow night and checked my notes= . I know I didn't mention any for AL so far this year and I don't have = anything for them in the near future. I always mention the state meetin= gs. Yours, Dave ______________________________= __ From: Amber McKenzie To: histonet@= lists.utsouthwestern.edu Sent: Thu, June 23, 2011 4:36:54 PM Subject:= [Histonet] AL state meeting? Does anyone know if there's an AL = state meeting this year? ____________________________________= ___________ Histonet mailing list Histonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/ma ilman/listinfo/histonet ----= -------------------------- Message: 13 Date: Sat, 25 Jun 2011 23:= 14:07 -0600 From: WILLIAM DESALVO Subject:= RE: [Histonet] How many tissues an histo tech is suppose to cut per = To: , histonet Message-ID: Content-= Type: text/plain; charset=3D"iso-8859-1" I understand your frust= ration and think that the lab you work at, or any lab, that uses production= standards MUST minimally explain how the standard is set and provide you a= training and competency plan/schedule to attain the standard. If that has = not been provided, I suggest you request a conversation with your Human Res= ources representative and/or the Lab Manager. You must have a clear underst= anding of the expectations set for you. That said, I do not understand why = any lab would set the standard at microtomy by number of blocks cut, blocks= should be used when setting the embedding task standard. I believe the cor= rect unit of measure is the slide. My experience in the Histology lab is th= at every block can be different (some GI or Prostate labs are an exception)= and even if they are not, the number of slides produced is a more accurate= unit to count. My MAJOR concern is setting a production standard without c= oupling it with a QUALITY standard. Production standards mean nothing if the highest quality cannot be attained and maintained throughout the lengt= h of task performance. The development of Production and Quality Standards = should be a reflection of the entire group of trained and competent employe= es performing the task. This includes ALL employees, new hire to highest le= vel of competency. I also think it a mistake to set task Standards at a max= imum level. Task standards should set as a minimum expectation level with e= ncouragement and reward to perform above standard. The goal of developing P= roduction/Quality Standards must always be employee improvement and reducti= on in defects/errors which will always lead to improved patient care. <= BR>William DeSalvo, B.S., HTL(ASCP) > From: joanne0658@comcast.ne= t > To: histonet@lists.utsouthwestern.edu > Date: Sat, 25 Jun 2= 011 19:07:07 -0400 > Subject: [Histonet] How many tissues an histo te= ch is suppose to cut per > > i am quite serious in my presenta= tion and request for advice. i too thought this goal was/is ridiculous to e= xpect/ask for from someone so new and to attain in 6 months or less. last m= onday i embedded 214 blocks and cut 148 between 5am and 10:30am (we had alm= ost 600 cassettes to share among 3 people) . . . .for someone so very new i= thought this pretty good . . . please note: most days aren't as hectic. :)= what is an average though for blocks/minute? what is meant by set sum per = block? <---keeping in mind i am new to this field. > > = > > _______________________________________________ > Histo= net mailing list > Histonet@lists.utsouthwestern.edu > http://l= ists.utsouthwestern.edu/mailman/listinfo/histonet --------------= ---------------- Message: 14 Date: Sun, 26 Jun 2011 08:31:03 -040= 0 From: "histotech@imagesbyhopper.com" Subject: Re: [Histonet] How many tissues an histo tech is suppose to cu= t per To: Victoria Baker Cc: "histonet@lists= .utsouthwestern.edu" , Joanne Message-ID: <82CEFE68-52CA-40D1-94A7-77012EAFE3E7@i= magesbyhopper.com> Content-Type: text/plain; charset=3Dus-ascii To ensure that I am understood correctly: I agree that the expectations f= or Joanne are unreasonable and unfair. I have read here some replies= that more acurately explain what I thought better than *I* did! - = Speed (while maintaining quality) will come over time. One *must* have qual= ity or speed is useless. - monitoring the slide output can be utilized a= s a tool towards showing positive progress. This could simply be cutting on= e more block/slide per day. There will come a time when one more just canno= t reasonably be done, but at least striving towards it gives something to s= hoot for. I recall a conversation I had with a coworker some years ago with= regards to embedding: (her) you should be able to improve by one block per= day. (me) Really? At that rate we will only need one person, as they will = be able to do it all! Sent from my iPhone On Jun 25, 2011, at= 9:57 PM, Victoria Baker wrote: > Joanne = - > > I have to agree with Tom and Barry in questioning how th= ey have come to this > time per block and the time frame with which t= hey expect you to do this in. > I was astonished at what I read in yo= ur second e-mail in terms of what you > did on that one day in only s= ix hours - do you still have all ten of your > fingers!!!!! I'm not m= aking fun - the pressure can be incredible when you > first start in = this field. > > I did a little internet searching and found so= me interesting information. > 'Histology workload standards' brought = up a lot of information and > publications regarding standards for pr= oductivity. Rene Buesa had > several publications that had good infor= mation and could give you a chance > to compare your lab to what is o= ut there in reasonable expections of > productivity. The ASCP website= didn't open up for me tonight (probably out > for coffee), and I did= n't go to NSH website which has other links. Use the > internet to gi= ve yourself some information and then approach things from > there.> > Hope this helps you some. I'm old school, learn the techniq= ues first, the > speed will fall into place and quality won't be at r= isk. > > Vikki > > > > > On = Sat, Jun 25, 2011 at 9:01 PM, Thomas Jasper wrote: >= ; >> Joanne, >> >> In my humble opinion and wi= thout knowing anything about where you work, >> this expectation i= s ludicrous. I have a hard time believing that >> anyone, regardle= ss of experience could attain the goal you've mentioned >> (includ= ing answering phones and running instruments). There are a >> mult= itude of reasons why this is absurd. Suffice it to say, I would be >&= gt; suspect of any work coming out of such an operation, not to mention = >> those in charge and the poor souls trying to meet this goal. &g= t;> >> I seriously doubt you'll be able to have much say about= things since you >> are so new. My advice...get out and find a jo= b working for realistic >> people. >> >> Good l= uck, >> Tom Jasper >> >> Thomas Jasper HT (ASCP= ) BAS >> Histology Supervisor >> Central Oregon Regional = Pathology Services >> Bend, Oregon 97701 >> 541/693-2677<= BR>>> tjasper@copc.net >> >> -----Original Message= ----- >> From: histonet-bounces@lists.utsouthwestern.edu >&g= t; [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne>> Sent: Saturday, June 25, 2011 2:50 PM >> To: histonet@l= ists.utsouthwestern.edu >> Subject: [Histonet] How many tissues an= histo tech is suppose to cut per >> >> >> >> i've only been working 2 months. although older, i am new as a >> histotech (graduated in may 2010, found a job in april 2011). see= ms >> management is setting a goal of a block per minute as far as= cutting >> goes for me. i have until october to attain this goal.= this minute for >> cutting is to include facing, writing out slid= es, cutting, and putting >> tray into symphony stainer (not to men= tion getting up to answer the >> phone, fielding questions regardi= ng send-out cases, and other slight >> "cutting interruptions). th= is seems an extreme, possibly unattainable >> goal. i'm up for a c= hallenge at age 53, but any advice would be >> SWONDERFUL!!!! :)>> >> _______________________________________________ = >> Histonet mailing list >> Histonet@lists.utsouthwestern.ed= u >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet = >> >> >> >> ___________________________= ____________________ >> Histonet mailing list >> Histonet= @lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailm= an/listinfo/histonet >> > _________________________________= ______________ > Histonet mailing list > Histonet@lists.utsouth= western.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histon= et > ------------------------------ Message: 1= 5 Date: Sun, 26 Jun 2011 08:19:17 -0500 From: "Horn, Hazel V" Subject: RE: [Histonet] Leica Bond for IHCs To: "'S= heila Adey'" , "histonet@lists.utsouthwestern.edu" = Message-ID: <25A4DE08332B19499= 904459F00AAACB7198B85AC74@EVS1.archildrens.org> Content-Type: text/pl= ain; charset=3D"us-ascii" We love ours and service has been excellen= t along with technical support. Hazel Horn Hazel Horn, HT/HTL (AS= CP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's= Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phon= e 501.364.4240 fax 501.364.3155 visit us on the web at: www.archi= ldrens.org -----Original Message----- From: histonet-bounces@list= s.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On = Behalf Of Sheila Adey Sent: Saturday, June 25, 2011 11:01 AM To: hist= onet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond for IHCs Hello netters: Looking for opinions on the Leica Bond immuno st= ainer please. Thanks. Sheila ______________________________________= _________ Histonet mailing list Histonet@lists.utsouthwestern.edu = http://lists.utsouthwestern.edu/mailman/listinfo/histonet **********= ******************************************************************* ********= ******************************************************************* ********= ******************************************************************* ********= ******************************************************************* ********= ******************************************************************* ********= ******************************************************************* ********= ******************************************************************* ********= ******************************************************************* ********= ******************** The information contained in this message may be pr= ivileged and confidential and protected from disclosure. If the reader o= f this message is not the intended recipient, or an employee or agent r= esponsible for delivering this message to the intended recipient, you a= re hereby notified that any dissemination, distribution or copying of t= his communication is strictly prohibited. If you have received this com= munication in error, please notify us immediately by replying to the me= ssage and deleting it from your computer. Thank you. ----= -------------------------- Message: 16 Date: Sun, 26 Jun 2011 07:= 22:31 -0700 (PDT) From: Rene J Buesa Subject: Re:= [Histonet] How many tissues an histo tech is suppose to cut per To: = histonet@lists.utsouthwestern.edu, Joanne Messa= ge-ID: <110225.86399.qm@web65710.mail.ac4.yahoo.com> Content-Type:= text/plain; charset=3Diso-8859-1 Embedding =3D 60 blocks/hour; cutt= ing =3D 24 blocks/hour. These are averages and many HTs embed/cut far more&= nbsp;or far less.There are no "beginner's standards". Ren=C3=A9 J. --- On Sat, 6/25/11, Joanne wrote: Fro= m: Joanne Subject: [Histonet] How many tissues = an histo tech is suppose to cut per To: histonet@lists.utsouthwestern.ed= u Date: Saturday, June 25, 2011, 7:07 PM i am quite serious i= n my presentation and request for advice. i too thought this goal was= /is ridiculous to expect/ask for from someone so new and to attain in 6 mon= ths or less. last monday i embedded 214 blocks and cut 148 between 5a= m and 10:30am (we had almost 600 cassettes to share among 3 people) . . . .= for someone so very new i thought this pretty good . . . please note: most = days aren't as hectic. :) what is an average though for blocks/= minute? what is meant by set sum per block? <---keeping in mind i = am new to this field. ______________________________________= _________ Histonet mailing list Histonet@lists.utsouthwestern.edu = http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------= ------------------------ Message: 17 Date: Sun, 26 Jun 2011 07:42= :59 -0700 (PDT) From: Rene J Buesa Subject: Fw: R= e: [Histonet] How many tissues an histo tech is suppose to cut per To= : histonet-request@lists.utsouthwestern.edu, histonet@lists.utsouthweste= rn.edu Message-ID: <643218.30016.qm@web65705.mail.ac4.yahoo.com>Content-Type: text/plain; charset=3Diso-8859-1 --- = Joanne: Read the attachment so you can have an idea = about productivity ranges and averages in different tasks in the lab. If= you embedded 214 blocks and cut 148 in 5.5 hours, using the averages= I provided, embedding should have taken 3.5 hours and cutting 6.2 hours wh= ich means you worked 1.76 times FASTER than the expected average. Ren=C3= =A9 J. --- On Sat, 6/25/11, Joanne wrote: From: Joanne Subject: [Histonet] How m= any tissues an histo tech is suppose to cut per To: histonet@lists.utsou= thwestern.edu Date: Saturday, June 25, 2011, 7:07 PM i am qui= te serious in my presentation and request for advice. i too thought t= his goal was/is ridiculous to expect/ask for from someone so new and to att= ain in 6 months or less. last monday i embedded 214 blocks and cut 14= 8 between 5am and 10:30am (we had almost 600 cassettes to share among 3 peo= ple) . . . .for someone so very new i thought this pretty good . . . please= note: most days aren't as hectic. :) what is an average though= for blocks/minute? what is meant by set sum per block? <---keepin= g in mind i am new to this field. __________________________= _____________________ Histonet mailing list Histonet@lists.utsouthwes= tern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Sun, 26 Ju= n 2011 12:38:28 -0400 From: Denise Mattingly Subject: [Histonet] Sakura auto TEC and the Leica cassette printer To: = histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=3DISO-885= 9-1 Any out there using an auto TEC embedder and the Leica cassette = Printer . We are having trouble with the paraform cassettes tumbling= out of the shoot and getting hung up . Sakura wont help because = its a Leica printer and Leica wont help because its a Sakura cassette Anyone have any suggestions?? Other than have someone flick the casset= te so it fall down the shoot properly ? Any help or suggestions g= reatly appreciated Denise Mattingly Riverside Hospital Columbu= s OH. 614-566-5679 ------------------------------ ____= ___________________________________________ Histonet mailing list His= tonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/l= istinfo/histonet End of Histonet Digest, Vol 91, Issue 35 *******= ********************************* From Timothy.Morken <@t> ucsfmedctr.org Mon Jun 27 10:15:00 2011 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Jun 27 10:15:15 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: <498DDA84FDA449B284C69014FBF60684@JoannePC> References: AANLkTimSmJ6JO=uRvj_djA-3h5Q5gjf2p2fEEG5mjtnE@mail.gmail.com <498DDA84FDA449B284C69014FBF60684@JoannePC> Message-ID: The only time I have seen techs cut that fast was when there was a team - one cuts, the other picks up the section and labels the slides. These were Aussies and Kiwis I worked with overseas and that is how they work in some places. BTW, they also stand at the bench and put the microtome sideways in order to work faster (pull the ribbon off, throw it on the waterbath and go on to the next block. Otherwise, forget it, it is not possible and still keep quality and sanity intact. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Jun 27 10:25:54 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jun 27 10:25:58 2011 Subject: [Histonet] Interesting article on a Healthcare Camp to educate children on the healthcare professions. Message-ID: Hi Histonetters, I found this article on a healthcare camp to educate kids on the healthcare professions: http://billingsgazette.com/news/state-and-regional/wyoming/article_06923 0e4-c77e-5b4d-95e0-bd9741f0595f.html Have a great day! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From JWeems <@t> sjha.org Mon Jun 27 10:45:31 2011 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Jun 27 10:45:36 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: References: AANLkTimSmJ6JO=uRvj_djA-3h5Q5gjf2p2fEEG5mjtnE@mail.gmail.com <498DDA84FDA449B284C69014FBF60684@JoannePC> Message-ID: <92AD9B20A6C38C4587A9FEBE3A30E164082116E941@CHEXCMS10.one.ads.che.org> At one place I worked we teamed like this and it was so efficient! It also helped with error reduction. The main problem I see for Joanne is the "getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions". No one can predict how long these interruptions would take. Joanne, I think I would ask for uninterrupted time in the beginning to hone your cutting skills. As your confidence builds, so will your speed. And so will the time to refocus after an interruption. I rarely cut these days and when I do I am slower than slow. Best wishes, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, June 27, 2011 11:15 To: 'Joanne'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per The only time I have seen techs cut that fast was when there was a team - one cuts, the other picks up the section and labels the slides. These were Aussies and Kiwis I worked with overseas and that is how they work in some places. BTW, they also stand at the bench and put the microtome sideways in order to work faster (pull the ribbon off, throw it on the waterbath and go on to the next block. Otherwise, forget it, it is not possible and still keep quality and sanity intact. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Kathy.Bonness <@t> UTSouthwestern.edu Mon Jun 27 11:22:25 2011 From: Kathy.Bonness <@t> UTSouthwestern.edu (Kathy Bonness) Date: Mon Jun 27 11:22:34 2011 Subject: [Histonet] Histogel Protocol FROZEN Histonet Digest, Vol 91, Issue 34 Message-ID: <2E046FFADED6054CB6DEB42B1B3E09642299F9@swmsmail6.swmed.org> Katy (or anyone else who has used Histogel for FROZEN samples), I am using Histogel mixed with OCT to create a frozen cell pellet. I was successful with doing a single pellet but now I am being asked to do multiple and have found hit some challenges. I have never done a TMA but that sounds like a great option. We are a research lab but using the Cell Pellet for a normalizing control of IF intensity (for computational analysis normalization for lung tissue) and one of the largest challenges is retaining cell density while still trying to cryoprotect and freeze well with the OCT surrounding it. Has anyone worked out a method for frozen cell pellets to embedded with multiple cell lines? Most of what I have found has been from FFPE, any suggestions would be appreciated. Thank you! Kathy Kathy M. Bonness, PhD. 251-533-2661 kathy.bonness@utsouthwestern.edu http://www.linkedin.com/pub/kathy-bonness/6/1a1/931 UTSW Dallas, TX Green Center for Computational & Systems Biology Department of Pharmacology Altschuler/Wu Lab (ND9.214) ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Saturday, June 25, 2011 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 91, Issue 34 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: HistoGel (Milne, Katy) 2. Histogel Problem (Amos Brooks) 3. RE: Histogel Problem (Delossantos_Roseann) 4. bluing (Webb, Dorothy L) 5. Re: bluing (Rene J Buesa) 6. RE: bluing (Harrison, Sandra C.) 7. blades (Webb, Dorothy L) 8. Re: blades (Sean McBride) 9. Re: blades (Esther C Peters) 10. Re: blades (Victoria Baker) 11. Re: blades (Jennifer MacDonald) 12. HT Position - Irvine, CA (Eric Velazquez) 13. Re: HT Position - Irvine, CA (Lee & Peggy Wenk) 14. Contents of Histonet digest (Aurea Marquez) 15. Re: bluing (Lee & Peggy Wenk) 16. Leica Bond for IHCs (Sheila Adey) 17. RE: Bluing (gayle callis) ---------------------------------------------------------------------- Message: 1 Date: Fri, 24 Jun 2011 10:27:09 -0700 From: "Milne, Katy" Subject: [Histonet] RE: HistoGel To: "'histonet@lists.utsouthwestern.edu'" , "'mjdessoye@wvhcs.org'" Message-ID: <3FEFF18FF4E1914A9AB7D8498591BE8610CF058850@VEXCCR02.phsabc.ehcnet.ca> Content-Type: text/plain; charset="us-ascii" We use histogel a lot in our lab. It's a research lab and we use it for a few purposes - pelleting cultured cells then creating multi-culture TMAs for testing antibodies and also pelleting cells from ascites and pleural effusions. Has also been used to process really small samples that could have been lost in the processor through the cassettes. Works quite well. The researchers just put the samples in histogel and give it to me in formalin then I process it as I would regular tissue. Cuts very well too. Katy Message: 3 Date: Fri, 24 Jun 2011 12:26:46 -0400 From: "Dessoye, Michael J" Subject: [Histonet] HistoGel To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello, Does anyone out there have any experience with HistoGel? It's Richard Allan/Thermo Fisher. They claim that you can "embed" scant tissues in the gel and then process, embed, and cut as usual. Just wondering how it works in the real world.... Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 ------------------------------ Message: 2 Date: Fri, 24 Jun 2011 13:29:09 -0400 From: Amos Brooks Subject: [Histonet] Histogel Problem To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, I have a problem with some blocks that were prepared with Histogel. I was hoping someone else might have had a similar problem and figured it out. I took a photo of the blocks that were mads and put them in a Picassa album here: https://picasaweb.google.com/lh/photo/APO3HsIMa2_jPOEs3QRGUjhz3qi22FNPb2i5JJnBCAk?feat=directlink The long & short of it is that the blocs were prepared by the researcher for me to process. They are mouse kidneys. Now it is entirely possible for him to have goofed something up in preparing the Histogel blocks. I wasn't there when he did it, but when I looked at them before processing, they all looked fine. (Like the adjacent good one in the photo). When they were processed they were placed in the VIP right next to each other. When I went to embed them this morning all but one of the four looked fine. The one that didn't come out well looked like the Histogel had shrunk up and shriveled around the kidneys. I am sure this will be aweful to cut, and the researcher is going to have a bird over it since this happened with another project previously. I would like to have a decent explanation for him, so if anyone knows what might have happened and has suggestions I would love to hear it (yes vendors too are welcome to answer this of course!). By the way, this was processed on a rather short cycle of 15-20 min per station of graded ETOH from 70% to 100% with 3 xylene stations and 4 paraffin stations (45 min for these). It seems fine for everything else that was on the processor. Just that one Histogel block was the issue. Thank you, Amos ------------------------------ Message: 3 Date: Fri, 24 Jun 2011 10:47:03 -0700 From: Delossantos_Roseann Subject: RE: [Histonet] Histogel Problem To: Amos Brooks Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <6E159393AFE18142A83CE7C2475AD96D0916498C5A@AGMAILCL100.allergan.com> Content-Type: text/plain; charset="us-ascii" Hi, I've encountered this problem before in my previous lab. To reduce the Histogel from shrinking that badly, avoid putting the tissue at the very edge of the Histogel, space them out nicely in the middle, providing sufficient amount of extra Histogel between each tissue and surrounding them. A little bit of shrinking usually do not interfere with cutting as long as the whole thing is placed flat on the final paraffin block, the rest will stretch out on the water bath. I find sometimes the gel does not behave well in the water but as long as it is not on top of your tissue when placed on your slide, it should interfere much with staining. Rose -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, June 24, 2011 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histogel Problem Hi, I have a problem with some blocks that were prepared with Histogel. I was hoping someone else might have had a similar problem and figured it out. I took a photo of the blocks that were mads and put them in a Picassa album here: https://picasaweb.google.com/lh/photo/APO3HsIMa2_jPOEs3QRGUjhz3qi22FNPb2i5JJnBCAk?feat=directlink The long & short of it is that the blocs were prepared by the researcher for me to process. They are mouse kidneys. Now it is entirely possible for him to have goofed something up in preparing the Histogel blocks. I wasn't there when he did it, but when I looked at them before processing, they all looked fine. (Like the adjacent good one in the photo). When they were processed they were placed in the VIP right next to each other. When I went to embed them this morning all but one of the four looked fine. The one that didn't come out well looked like the Histogel had shrunk up and shriveled around the kidneys. I am sure this will be aweful to cut, and the researcher is going to have a bird over it since this happened with another project previously. I would like to have a decent explanation for him, so if anyone knows what might have happened and has suggestions I would love to hear it (yes vendors too are welcome to answer this of course!). By the way, this was processed on a rather short cycle of 15-20 min per station of graded ETOH from 70% to 100% with 3 xylene stations and 4 paraffin stations (45 min for these). It seems fine for everything else that was on the processor. Just that one Histogel block was the issue. Thank you, Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail, including any attachments, is meant only for the intended recipient and may be a confidential communication or a communication privileged by law. If you received this e-mail in error, any review, use, dissemination, distribution, or copying of this e-mail is strictly prohibited. Please notify the sender immediately of the error by return e-mail and please delete this message from your system. Thank you in advance for your cooperation.

------------------------------ Message: 4 Date: Fri, 24 Jun 2011 14:20:33 -0500 From: "Webb, Dorothy L" Subject: [Histonet] bluing To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD7D@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Looking to change my "bluing" step in the H&E process to obtain a bluer (less purple) hue to the nuclear detail. What is everyone using in their bluing step?? Thanks for all of your ideas!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ------------------------------ Message: 5 Date: Fri, 24 Jun 2011 12:35:18 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] bluing To: "'histonet@lists.utsouthwestern.edu'" , Dorothy LWebb Message-ID: <201322.12289.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 There are several "bluing solutions" in the market, or you could use lithium carbonate at different concentrations until you find one of your liking. Ren? J. --- On Fri, 6/24/11, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] bluing To: "'histonet@lists.utsouthwestern.edu'" Date: Friday, June 24, 2011, 3:20 PM Looking to change my "bluing" step in the H&E process to obtain a bluer (less purple) hue to the nuclear detail.? What is everyone using in their bluing step?? Thanks for all of your ideas!! ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 24 Jun 2011 15:33:12 -0500 From: "Harrison, Sandra C." Subject: RE: [Histonet] bluing To: "Webb, Dorothy L" , Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Dorothy, Try Richard Allan Bluing Reagent. Here's what they say about their product: "It is a buffered product that ensures the proper alkalinity (pH=8.0). Unlike ammonia and lithium carbonate, RA's Bluing Reagent does not allow for the pH shift which can affect the crispness of nuclear detail." When I first began supervising this lab 5 years ago, they "made from scratch" their own hematoxylin and eosin (not to mention buffered formalin.) Unfortunately, the quality of the stain was very spotty and caused the Pathologists a lot of problems. I switched us to the Richard Allan 7211 Hematoxylin, which has beautiful, crisp nuclear detail. We also went with the recommended Richard Allan Clarifier, Bluing and Eosin, as well, so that we could produce a consistently high quality H&E every time. In 5 years, we've had very few complaints about the stain from the 8-10 Pathologists we've worked with, except for one occasion when there was some isolated nuclear hazing. We did some detective work and determined that the cause was due to a rack or two that had been placed in the oven without properly removing the excess water or draining of the slides before placing them in the oven. Have a great week-end everybody. It's practically the 1st sunny day we've had, here in Minneapolis, for the past 2 weeks and Saturday and Sunday's forecast looks good, too! Sandy Harrison VA-Minneapolis Supervisor, Anatomical and Surgical Pathology 612-467-2449 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, June 24, 2011 2:21 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bluing Looking to change my "bluing" step in the H&E process to obtain a bluer (less purple) hue to the nuclear detail. What is everyone using in their bluing step?? Thanks for all of your ideas!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 24 Jun 2011 15:53:39 -0500 From: "Webb, Dorothy L" Subject: [Histonet] blades To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43010F8BFEBD81@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Trying to clean up some things hanging out there in our lab and wondering what everyone does with a blade that has been used minimally and tech done for the day with the microtome. Where do you store that blade for use tomorrow or do you toss and not worry about the cost involved? I do not like them sitting on top of the microtome. Any good ideas?? Thanks, as always! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ------------------------------ Message: 8 Date: Fri, 24 Jun 2011 17:16:06 -0400 From: Sean McBride Subject: Re: [Histonet] blades To: "Webb, Dorothy L" Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <0FA5973D-A2CA-4364-BD5E-A477F302BD8D@andrew.cmu.edu> Content-Type: text/plain; charset=us-ascii Dorothy, I put ours in a 15 mL centrifuge tube with a cap & sit it on the base of the microtome for the next use, that way, no one gets cut & the blade is able to be used to the fullest of it's potential. :-) Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu On Jun 24, 2011, at 4:53 PM, Webb, Dorothy L wrote: > Trying to clean up some things hanging out there in our lab and wondering what everyone does with a blade that has been used minimally and tech done for the day with the microtome. Where do you store that blade for use tomorrow or do you toss and not worry about the cost involved? I do not like them sitting on top of the microtome. Any good ideas?? Thanks, as always! > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Fri, 24 Jun 2011 17:10:30 -0400 From: Esther C Peters Subject: Re: [Histonet] blades To: "Webb, Dorothy L" Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset=us-ascii We put ours in a small slide box (plastic or styrofoam, 5-25 slides) that is clearly marked "Microtome Blades for Facing Blocks" to be used another day. Esther C. Peters, Ph.D. Assistant Professor Department of Environmental Science & Policy Biology Program/Medical Technology Coordinator George Mason University 4400 University Drive, MSN 5F2 Fairfax, VA 22030-4444 Office: David King Hall 3057 Phone: 703-993-3462 Fax: 703-993-1066 epeters2@gmu.edu ----- Original Message ----- From: "Webb, Dorothy L" Date: Friday, June 24, 2011 4:53 pm Subject: [Histonet] blades > Trying to clean up some things hanging out there in our lab and > wondering what everyone does with a blade that has been used > minimally and tech done for the day with the microtome. Where do > you store that blade for use tomorrow or do you toss and not worry > about the cost involved? I do not like them sitting on top of the > microtome. Any good ideas?? Thanks, as always! > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and > are intended solely for the use of the individual or entity to > whom they are addressed. If you are not the intended recipient or > the individual responsible for delivering the e-mail to the > intended recipient, please be advised that you have received this > e-mail in error and that any use, dissemination, forwarding, > printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately > notify the HealthPartners Support Center by telephone at (952) 967- > 6600. You will be reimbursed for reasonable costs incurred in > notifying us. HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Fri, 24 Jun 2011 17:31:33 -0400 From: Victoria Baker Subject: Re: [Histonet] blades To: Esther C Peters Cc: "histonet@lists.utsouthwestern.edu" , "Webb, Dorothy L" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I use the cardboards that come in a box of slides. A small piece of tape on the open side and mark it used. I just always make sure I have the blade edge facing the folded part. I know some who will tape this folded board it to the side of their microtome and use it as a trimming blade holder. Vikki On Fri, Jun 24, 2011 at 5:10 PM, Esther C Peters wrote: > We put ours in a small slide box (plastic or styrofoam, 5-25 slides) that > is clearly marked "Microtome Blades for Facing Blocks" to be used another > day. > > Esther C. Peters, Ph.D. > Assistant Professor > Department of Environmental Science & Policy > Biology Program/Medical Technology Coordinator > George Mason University > 4400 University Drive, MSN 5F2 > Fairfax, VA 22030-4444 > Office: David King Hall 3057 > Phone: 703-993-3462 > Fax: 703-993-1066 > epeters2@gmu.edu > > ----- Original Message ----- > From: "Webb, Dorothy L" > Date: Friday, June 24, 2011 4:53 pm > Subject: [Histonet] blades > > > Trying to clean up some things hanging out there in our lab and > > wondering what everyone does with a blade that has been used > > minimally and tech done for the day with the microtome. Where do > > you store that blade for use tomorrow or do you toss and not worry > > about the cost involved? I do not like them sitting on top of the > > microtome. Any good ideas?? Thanks, as always! > > > > > > > > ________________________________ > > This e-mail and any files transmitted with it are confidential and > > are intended solely for the use of the individual or entity to > > whom they are addressed. If you are not the intended recipient or > > the individual responsible for delivering the e-mail to the > > intended recipient, please be advised that you have received this > > e-mail in error and that any use, dissemination, forwarding, > > printing, or copying of this e-mail is strictly prohibited. > > > > If you have received this e-mail in error, please immediately > > notify the HealthPartners Support Center by telephone at (952) 967- > > 6600. You will be reimbursed for reasonable costs incurred in > > notifying us. HealthPartners R001.0 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 11 Date: Fri, 24 Jun 2011 14:50:43 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] blades To: "Webb, Dorothy L" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <9A2E9444-36FF-418C-A794-AB6531E953AD@mtsac.edu> Content-Type: text/plain; charset=us-ascii We use plastic 5 slide mailers Sent from my iPhone On Jun 24, 2011, at 1:53 PM, "Webb, Dorothy L" wrote: > Trying to clean up some things hanging out there in our lab and wondering what everyone does with a blade that has been used minimally and tech done for the day with the microtome. Where do you store that blade for use tomorrow or do you toss and not worry about the cost involved? I do not like them sitting on top of the microtome. Any good ideas?? Thanks, as always! > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Fri, 24 Jun 2011 16:56:32 -0700 From: Eric Velazquez Subject: [Histonet] HT Position - Irvine, CA To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=windows-1252 Hi Histonet, We are currently looking for a full-time Histotechnician (preferably HT certified) with experience in IHC. Please read the description below and if you're interested please submit your resume via e-mail to: * eric.velazquez@agendia.com*. *ESSENTIAL DUTIES AND RESPONSIBILITIES* - Performs histology aspects of the lab, which includes cutting tissue(frozen, FFPE), manually staining sections for H&E imaging, IHC staining, loading slides on ScanScope for imaging and scoring by pathologist - Maintains laboratory logs so that current information is complete and readily available to staff, including clinical log sheet, ScanScope identification - Performs all aspects of lab support to ensure timely completion of samples. Reports any discrepancies to the Director Of Laboratory Operations. - Performs various maintenance and/or housekeeping tasks to keep laboratory clean, and ensure ease of use. - Cleans and fills water baths, bulk reagents, dump waste containers - Reports any malfunctions of equipment to the Director Of Laboratory Operations. - Washes all laboratory dishes to ensure a clean supply when needed. - Performs data entry tasks in the laboratory and office when needed. - Follows all Agendia, Inc.?s health and safety policies and procedures - Performs other related duties as required or assigned Thank you for your interest. -Eric Velazquez Agendia Inc. ------------------------------ Message: 13 Date: Sat, 25 Jun 2011 10:10:17 -0400 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] HT Position - Irvine, CA To: "Eric Velazquez" , Message-ID: <803AB1A1F69C40708BC7795115D1CFB7@HP2010> Content-Type: text/plain; format=flowed; charset="Windows-1252"; reply-type=original Where are you? City, State, Country, Lab, etc. Info, please. -----Original Message----- From: Eric Velazquez Sent: Friday, June 24, 2011 7:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT Position - Irvine, CA Hi Histonet, We are currently looking for a full-time Histotechnician (preferably HT certified) with experience in IHC. Please read the description below and if you're interested please submit your resume via e-mail to: * eric.velazquez@agendia.com*. *ESSENTIAL DUTIES AND RESPONSIBILITIES* - Performs histology aspects of the lab, which includes cutting tissue(frozen, FFPE), manually staining sections for H&E imaging, IHC staining, loading slides on ScanScope for imaging and scoring by pathologist - Maintains laboratory logs so that current information is complete and readily available to staff, including clinical log sheet, ScanScope identification - Performs all aspects of lab support to ensure timely completion of samples. Reports any discrepancies to the Director Of Laboratory Operations. - Performs various maintenance and/or housekeeping tasks to keep laboratory clean, and ensure ease of use. - Cleans and fills water baths, bulk reagents, dump waste containers - Reports any malfunctions of equipment to the Director Of Laboratory Operations. - Washes all laboratory dishes to ensure a clean supply when needed. - Performs data entry tasks in the laboratory and office when needed. - Follows all Agendia, Inc.?s health and safety policies and procedures - Performs other related duties as required or assigned Thank you for your interest. -Eric Velazquez Agendia Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Sat, 25 Jun 2011 07:16:32 -0700 (PDT) From: Aurea Marquez Subject: [Histonet] Contents of Histonet digest To: histonet@lists.utsouthwestern.edu Message-ID: <462293.86417.qm@web120511.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ? Aurea Griselle Marquez ________________________________ From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Fri, June 24, 2011 1:01:21 PM Subject: Histonet Digest, Vol 91, Issue 33 Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. Formula R paraffin (Clare Thornton) ? 2. Re: Formula R paraffin (Rene J Buesa) ? 3. HistoGel (Dessoye, Michael J) ? 4. (no subject) (Kendra) ---------------------------------------------------------------------- Message: 1 Date: Fri, 24 Jun 2011 12:08:02 -0400 From: Clare Thornton Subject: [Histonet] Formula R paraffin To: "'Histonet@lists.utsouthwestern.edu'" ??? Message-ID: ??? Content-Type: text/plain; charset="us-ascii" Does anyone have any experience with Formula R paraffin, from Leica, good or bad?? We currently use Paraplast X-tra but occasionally have troubles with compression, and we heard Formula R is harder and less likely to compress. Thanks! Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com ------------------------------ Message: 2 Date: Fri, 24 Jun 2011 09:21:35 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Formula R paraffin To: "'Histonet@lists.utsouthwestern.edu'" ??? , ??? Clare Thornton ??? Message-ID: <851084.79705.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If sometimes you are experiencing compression problems it could be because your paraffin has some xylene contamination, or your blocks are not cold enough when cutting, or you are using a paraffin of lower melting point as needed for the tissue you regularly process. Paraplast X-tra always worked fine for me. Check with them for a harder paraffin instead of changing to a different paraffin altogether. Ren? J. --- On Fri, 6/24/11, Clare Thornton wrote: From: Clare Thornton Subject: [Histonet] Formula R paraffin To: "'Histonet@lists.utsouthwestern.edu'" Date: Friday, June 24, 2011, 12:08 PM Does anyone have any experience with Formula R paraffin, from Leica, good or bad?? We currently use Paraplast X-tra but occasionally have troubles with compression, and we heard Formula R is harder and less likely to compress. Thanks! Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 24 Jun 2011 12:26:46 -0400 From: "Dessoye, Michael J" Subject: [Histonet] HistoGel To: Message-ID: ??? Content-Type: text/plain;??? charset="iso-8859-1" Hello, Does anyone out there have any experience with HistoGel?? It's Richard Allan/Thermo Fisher.? They claim that you can "embed" scant tissues in the gel and then process, embed, and cut as usual.? Just wondering how it works in the real world.... Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye@wvhcs.org ? | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ------------------------------ Message: 4 Date: Fri, 24 Jun 2011 11:28:55 -0500 From: Kendra Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: <723pa4ptd019lmddnpqhrxwt.1308932935185@email.android.com> Content-Type: text/plain; charset=utf-8 ------------------------------ Message: 15 Date: Sat, 25 Jun 2011 10:26:23 -0400 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] bluing To: "Webb, Dorothy L" , Message-ID: <2B7338AFC93A4F91AD215CDC80E1B201@HP2010> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original It might just need longer time in the bluing agent. Hematoxylin solutions stain the nuclei reddish (look at your slide right after coming out of hematoxylin - tissue will look reddish). The alkaline solutions that are used for "bluing" remove a H+ group on the aluminum hematein (that's the staining chemical of the hematoxylin solution), and change it to a -OH group. This changes the aluminum hematein from a reddish color to a blue color. The more alkaline (higher pH) the bluing agent, the faster this reaction and color change, and the less time is needed in the bluing agent. The lower the alkalinity (not as high pH but still in the alkaline range) the bluing agent, the slower the reaction and color change, and more time is needed in the bluing agent. Dilute ammonia water usually only takes a few seconds, as it's pH is usually high (pH 10). Tap water can have a pH of around 7, and may take 5-10 minutes to "blue". If the tap water is more acidic (pH 5 or below), the slides may not "blue". Now, we have to get all the nuclei from the reddish color to the bluish color. If the slide is not in the bluing agent for enough time (for the type/pH of bluing agent), then some of the nuclei change to blue, while some still remain reddish (or within a single nucleus, some of the DNA has changed to blue, some remains reddish), hence a more purple color. So, the easiest thing to try right now is to stain 2 slides from the same block (serial sections) in your hematoxylin for the same time, put one in the bluing agent for the usual time, put the other in the alkaline solution for extended time, and see if the nuclei on the second slide are now more blue than the first slide. Let us know. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: Webb, Dorothy L Sent: Friday, June 24, 2011 3:20 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bluing Looking to change my "bluing" step in the H&E process to obtain a bluer (less purple) hue to the nuclear detail. What is everyone using in their bluing step?? Thanks for all of your ideas!! ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Sat, 25 Jun 2011 12:00:44 -0400 From: Sheila Adey Subject: [Histonet] Leica Bond for IHCs To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello netters: Looking for opinions on the Leica Bond immuno stainer please. Thanks. Sheila ------------------------------ Message: 17 Date: Sat, 25 Jun 2011 10:47:15 -0600 From: "gayle callis" Subject: [Histonet] RE: Bluing To: "Histonet" Message-ID: <000101cc3357$8b945ff0$a2bd1fd0$@callis@bresnan.net> Content-Type: text/plain; charset="us-ascii" Dear All, We use commercial bluing reagents for convenience e.g. Richard Allan Bluing Reagent ready to use and have used Scotts Tap Water Substitute Concentrate diluted before use. We do not use ammonia water anymore as the high pH can cause section lift off from slide. Here is recipe for Scotts. SCOTT'S TAP WATER SUBSTITUTE (You can make this up as a 10X concentrate if you wish. Store in house concentrate at 4C or growth will occur) Sodium bicarbonate 2 g Magnesium sulfate (anhydrous) 20 g Tap Water 1 L Peggy presented a good explanation of bluing. We do not use tap water anymore due to incorrect pH. One of the failures for complete bluing is not changing the bluing reagent daily with large volumes of slides. Lower number of slides, one might be able to get by with fewer changes of bluing. Tap water rinses dilutes the reagent and can change the pH, which should be pH 8. If there is a large volume of slides, anything over 75 or more, we change the bluing reagent daily. It is important to remember that Clarifiers e.g. acetic acid reagents used with progressive hematoxylins (Richard Allan Hematoxylin 1, Gill 1,2,3) will suffer from tap water rinse dilution too. We also change those, and if Clarifier looks rather yellow, then you have probably exhausted the acetic acid and its effectiveness. Jerry Fredenburgh has given excellent workshops on hematoxylin staining in the past and passed on many of these facts. Consequently, we have no clarifying or bluing problems. Fredenburgh recommendations for Richard Allan Hematoxylin 1 staining procedure that we use: Hematoxylin 1 - 1.5 min, longer if needed Tap water rinse-1 minute Clarifier - 1 minute Tap water rinse - 1 minute Bluing - 1 minute Tap water rinse - 1 minute Remember, static or non-running water rinse stations are contaminated with carryover hematoxylin, clarifier and/or bluing reagent. If you do NOT remove the bluing reagent with a good rinse, then eosin staining is affected by cations carried over from the bluing reagent. We also have an alcohol step (matching % of alcohol in eosin) before going into this stain. This helps remove the cations plus prepares the section for eosin staining. Perform good rinses and change static water rinse stations after a run, or frequently during a day of heavy staining. The second and third racks of slides on a stainer should have the same tap water rinse conditions as the first rack. Yes, extra work but you need to ensure consistent staining results of H&E of ALL sections- the universal workhorse stain we depend on so heavily. Years ago, Richard Allan had an excellent manual for H&E staining guidelines on how to perform and adjust your H&E staining. Sadly the manual is no longer published but the good news is ...... I saved my copy, and scanned it to pdf if anyone wants it. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT **************************************************************************** ***************************************************************** Peggy Wenk wrote: It might just need longer time in the bluing agent. Hematoxylin solutions stain the nuclei reddish (look at your slide right after coming out of hematoxylin - tissue will look reddish). The alkaline solutions that are used for "bluing" remove a H+ group on the aluminum hematein (that's the staining chemical of the hematoxylin solution), and change it to a -OH group. This changes the aluminum hematein from a reddish color to a blue color. The more alkaline (higher pH) the bluing agent, the faster this reaction and color change, and the less time is needed in the bluing agent. The lower the alkalinity (not as high pH but still in the alkaline range) the bluing agent, the slower the reaction and color change, and more time is needed in the bluing agent. Dilute ammonia water usually only takes a few seconds, as it's pH is usually high (pH 10). Tap water can have a pH of around 7, and may take 5-10 minutes to "blue". If the tap water is more acidic (pH 5 or below), the slides may not "blue". Now, we have to get all the nuclei from the reddish color to the bluish color. If the slide is not in the bluing agent for enough time (for the type/pH of bluing agent), then some of the nuclei change to blue, while some still remain reddish (or within a single nucleus, some of the DNA has changed to blue, some remains reddish), hence a more purple color. So, the easiest thing to try right now is to stain 2 slides from the same block (serial sections) in your hematoxylin for the same time, put one in the bluing agent for the usual time, put the other in the alkaline solution for extended time, and see if the nuclei on the second slide are now more blue than the first slide. Let us know. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: Webb, Dorothy L Sent: Friday, June 24, 2011 3:20 PM To: 'histonet <@t> lists.utsouthwestern.edu' Subject: [Histonet] bluing Looking to change my "bluing" step in the H&E process to obtain a bluer (less purple) hue to the nuclear detail. What is everyone using in their bluing step?? Thanks for all of your ideas!! ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 91, Issue 34 **************************************** ________________________________ UT Southwestern Medical Center The future of medicine, today. From Pat.Patterson <@t> propath.com Mon Jun 27 11:46:57 2011 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Mon Jun 27 11:48:52 2011 Subject: [Histonet] IHC Position Dallas - Day Shift Message-ID: <82C7248978CB50469FD6BA68EBBEFE6705D74566@exchange.propathlab.com> ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 4 years Histology experience with at least 1-2 years immunohistochemistry, immunofluorescence or in situ hybridization experience. Working knowledge of IHC theory required, hands on IHC performance and cryostat sectioning experience with skin and kidney core biopsies desired. If using an automated IHC system we'll easily train you on our manual system. HT (ASCP) or QIHC desired. The hours for the position are 5:00 a.m. to 1:30 p.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! EOE Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibility@propath.com or call 214/237-1889. For consideration send resume to: ProPath, Human Resources 1355 River Bend Drive Dallas, TX 75247 Fax - 214/237-1825 Job Line - 214/237-1775 jobs@propath.com www.propath.com Pat Patterson, HTL(ASCP) Supervisor, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com The information contained in this message may be privileged and confidential. If you are NOT the intended recipient, please notify the sender immediately with a copy to Postmaster@propath.com and destroy this message. From lblazek <@t> digestivespecialists.com Mon Jun 27 12:10:11 2011 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Jun 27 12:10:16 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: References: AANLkTimSmJ6JO=uRvj_djA-3h5Q5gjf2p2fEEG5mjtnE@mail.gmail.com <498DDA84FDA449B284C69014FBF60684@JoannePC> Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBF65EA57@IBMB7Exchange.digestivespecialists.com> Unless you are in some remote area that there aren't any other facilities around, I would look for a new job! I don't think your age should have any bearing on finding one. If you were close to me I'd hire you. Working under that kind of condition is unacceptable in my opinion. It promotes errors and that isn't what we are all about. Those blocks are our patients. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lori.garcia <@t> medtronic.com Mon Jun 27 12:11:22 2011 From: lori.garcia <@t> medtronic.com (Garcia, Lori, Sr. Scientist) Date: Mon Jun 27 12:11:36 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: References: AANLkTimSmJ6JO=uRvj_djA-3h5Q5gjf2p2fEEG5mjtnE@mail.gmail.com <498DDA84FDA449B284C69014FBF60684@JoannePC> Message-ID: <5A8A2A45BE610D459A95CD6A9102102A0124CFD303@STSM1BMSGM04.ent.core.medtronic.com> I agree with Tim. I have been in the field for over 20 years, and this is an unrealistic goal even for a veteran. Your manager obviously has no experience or understanding of your job in order to set that type of goal for you. It would be possible if the blocks are already chilled, the slides pre-labeled, and you have a helper to load the slides in the racks and onto the stainer. Even then there will be some sacrifice of quality for quantity. Just my two cents. Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, June 27, 2011 8:15 AM To: 'Joanne'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per The only time I have seen techs cut that fast was when there was a team - one cuts, the other picks up the section and labels the slides. These were Aussies and Kiwis I worked with overseas and that is how they work in some places. BTW, they also stand at the bench and put the microtome sideways in order to work faster (pull the ribbon off, throw it on the waterbath and go on to the next block. Otherwise, forget it, it is not possible and still keep quality and sanity intact. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From rsrichmond <@t> gmail.com Mon Jun 27 12:18:21 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Jun 27 12:18:25 2011 Subject: [Histonet] Re: Histogel Message-ID: Histogel is indeed expensive, and often not permitted by Management for that reason. Preparing your own agar is a bit tricky - you really need a hot plate and a magnetic stirrer, unlikely items in a histology lab. (2% or 3% dry agar, in water.) Back before hospital microbiology turned into the black box it is today, you used to be able to walk across the hall and pick up a tube of trypticase soy agar (TSA - I think it was 3% agar with some stuff in it to make bugs grow) and use that. One way to use agar is for the pathologist to pour out some melted agar on a glass slide or metal ruler, and embed small specimens in it so they stay oriented - works great for temporal artery biopsies and vasectomy specimens - time consuming. A blood bank heating block is useful for keeping tubes of agar melted at the gross desk. Whether you use Histogel or some other agar, it's very important not to commit any valuable specimens to it until you're sure it works in your system. I like to carve out some "pseudo-biopsies" from a normal mucosa in a colon resection specimen and run them. - I've seen some disasters when this precaution wasn't taken. Bob Richmond Samurai Pathologist Knoxville TN From sgoebel <@t> mirnarx.com Mon Jun 27 12:51:55 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Mon Jun 27 12:52:04 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390EBF65EA57@IBMB7Exchange.digestivespecialists.com> References: AANLkTimSmJ6JO=uRvj_djA-3h5Q5gjf2p2fEEG5mjtnE@mail.gmail.com<498DDA84FDA449B284C69014FBF60684@JoannePC> <5A2BD13465E061429D6455C8D6B40E390EBF65EA57@IBMB7Exchange.digestivespecialists.com> Message-ID: I second this motion!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, June 27, 2011 12:10 PM To: 'Joanne'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per Unless you are in some remote area that there aren't any other facilities around, I would look for a new job! I don't think your age should have any bearing on finding one. If you were close to me I'd hire you. Working under that kind of condition is unacceptable in my opinion. It promotes errors and that isn't what we are all about. Those blocks are our patients. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon Jun 27 13:05:45 2011 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Jun 27 13:05:49 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cutper In-Reply-To: References: <643218.30016.qm@web65705.mail.ac4.yahoo.com> Message-ID: <25A4DE08332B19499904459F00AAACB7198B85AC7E@EVS1.archildrens.org> As a manager I would never expect that kind of turn around. I think it's impossible to achieve. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Monday, June 27, 2011 9:38 AM To: rjbuesa@yahoo.com; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: Re: [Histonet] How many tissues an histo tech is suppose to cutper Another solution...get out of clinical and go into research =) There are no quotas or slide per second expectations in the research world =) So sorry you are having such a bad time with your job. HT's are not a dime a dozen and usually it is fairly easy to find a better job. Not to mention research pays almost double the clinical world!! Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, June 26, 2011 9:43 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: Fw: Re: [Histonet] How many tissues an histo tech is suppose to cutper --- Joanne: Read the attachment so you can have an idea about productivity ranges and averages in different tasks in the lab. If you embedded 214 blocks and cut 148? in 5.5 hours, using the averages I provided, embedding should have taken 3.5 hours and cutting 6.2 hours which means you worked 1.76 times FASTER than the expected average. Ren? J. --- On Sat, 6/25/11, Joanne wrote: From: Joanne Subject: [Histonet] How many tissues an histo tech is suppose to cut per To: histonet@lists.utsouthwestern.edu Date: Saturday, June 25, 2011, 7:07 PM i am quite serious in my presentation and request for advice.? i too thought this goal was/is ridiculous to expect/ask for from someone so new and to attain in 6 months or less.? last monday i embedded 214 blocks and cut 148 between 5am and 10:30am (we had almost 600 cassettes to share among 3 people) . . . .for someone so very new i thought this pretty good . . . please note: most days aren't as hectic.? :)? what is an average though for blocks/minute?? what is meant by set sum per block? <---keeping in mind i am new to this field. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From ASelf <@t> georgetownhospitalsystem.org Mon Jun 27 13:10:59 2011 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Mon Jun 27 13:11:07 2011 Subject: [Histonet] Oncotype Message-ID: I was wandering how many of you out there are using oncotype dx. for some of your breast patients? And what is your handling process for this test? Thanks in advance, Amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From abeharry798 <@t> gmail.com Mon Jun 27 14:07:24 2011 From: abeharry798 <@t> gmail.com (Gmail) Date: Mon Jun 27 14:07:38 2011 Subject: [Histonet] Oncotype In-Reply-To: References: Message-ID: We do this testing for oncology. They pay for the shipping via fedex. The oncologist fills out the requsition and the lab pulls the block , packages it and ships it. Andrea On 2011-06-27, at 2:10 PM, Amy Self wrote: > I was wandering how many of you out there are using oncotype dx. for > some of your breast patients? And what is your handling process for > this test? > > > Thanks in advance, > Amy > > > Amy Self > Georgetown Hospital System > > NOTE: > The information contained in this message may be privileged, > confidential and protected from disclosure. If the reader of this > message is not the intended recipient, or an employee or agent > responsible for delivering this message to the intended recipient, > you are hereby notified that any dissemination, distribution or > copying of this communication is strictly prohibited. If you have > received this communication in error, please notify us immediately > by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sherene.cohen <@t> ventana.roche.com Mon Jun 27 14:38:22 2011 From: sherene.cohen <@t> ventana.roche.com (Cohen, Sherene) Date: Mon Jun 27 14:38:28 2011 Subject: [Histonet] Oncotype Message-ID: When I was working in the lab, we sent specimens for Oncotype. They perform. 21 gene assay to predict the chance of recurrence of the cancer within 10 years. You can either send the tissue block or slides (depending on your send out policy). The testing can have an impact on the patient's tratment. Sherene ----- Original Message ----- From: histonet-bounces@lists.utsouthwestern.edu To: 'histonet@lists.utsouthwestern.edu' Sent: Mon Jun 27 14:10:59 2011 Subject: [Histonet] Oncotype I was wandering how many of you out there are using oncotype dx. for some of your breast patients? And what is your handling process for this test? Thanks in advance, Amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Mon Jun 27 14:47:25 2011 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Mon Jun 27 14:47:29 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: References: <498DDA84FDA449B284C69014FBF60684@JoannePC> <5A2BD13465E061429D6455C8D6B40E390EBF65EA57@IBMB7Exchange.digestivespecialists.com> Message-ID: I have known one or two people who can attain this rate. However with them, it is not consistent, especially if there are any interruptions. I would take a couple of articles, especially some of Rene Buesa's authoritative articles to your management and encourage them to set realistic expectations. If your manager has any experience in histology, ask them to show you how to do it, (put them on the spot to try to meet their own goals). Good luck. On Mon, Jun 27, 2011 at 10:51 AM, wrote: > I second this motion!! > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, > Linda > Sent: Monday, June 27, 2011 12:10 PM > To: 'Joanne'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut > per > > Unless you are in some remote area that there aren't any other > facilities around, I would look for a new job! I don't think your age > should have any bearing on finding one. If you were close to me I'd > hire you. Working under that kind of condition is unacceptable in my > opinion. It promotes errors and that isn't what we are all about. > Those blocks are our patients. > > Linda > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne > Sent: Saturday, June 25, 2011 2:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] How many tissues an histo tech is suppose to cut per > > > > i've only been working 2 months. although older, i am new as a > histotech (graduated in may 2010, found a job in april 2011). seems > management is setting a goal of a block per minute as far as cutting > goes for me. i have until october to attain this goal. this minute for > cutting is to include facing, writing out slides, cutting, and putting > tray into symphony stainer (not to mention getting up to answer the > phone, fielding questions regarding send-out cases, and other slight > "cutting interruptions). this seems an extreme, possibly unattainable > goal. i'm up for a challenge at age 53, but any advice would be > SWONDERFUL!!!! :) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From trathborne <@t> somerset-healthcare.com Mon Jun 27 14:56:14 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Jun 27 14:57:06 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: References: <498DDA84FDA449B284C69014FBF60684@JoannePC> <5A2BD13465E061429D6455C8D6B40E390EBF65EA57@IBMB7Exchange.digestivespecialists.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570702D42C@SMCMAIL01.somerset-healthcare.com> To cut at this rate can cause carelessness, resulting in possible loss of specimen or tech injuries. I'm curious about your co-workers. What is their average/hour? Unless conditions are optimal (i.e. humidity, tissue processing, good blades, etc.), this will not be an easy goal to attain. Best of luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Monday, June 27, 2011 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per I have known one or two people who can attain this rate. However with them, it is not consistent, especially if there are any interruptions. I would take a couple of articles, especially some of Rene Buesa's authoritative articles to your management and encourage them to set realistic expectations. If your manager has any experience in histology, ask them to show you how to do it, (put them on the spot to try to meet their own goals). Good luck. On Mon, Jun 27, 2011 at 10:51 AM, wrote: > I second this motion!! > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Blazek, Linda > Sent: Monday, June 27, 2011 12:10 PM > To: 'Joanne'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] How many tissues an histo tech is suppose to > cut per > > Unless you are in some remote area that there aren't any other > facilities around, I would look for a new job! I don't think your age > should have any bearing on finding one. If you were close to me I'd > hire you. Working under that kind of condition is unacceptable in my > opinion. It promotes errors and that isn't what we are all about. > Those blocks are our patients. > > Linda > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne > Sent: Saturday, June 25, 2011 2:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] How many tissues an histo tech is suppose to cut > per > > > > i've only been working 2 months. although older, i am new as a > histotech (graduated in may 2010, found a job in april 2011). seems > management is setting a goal of a block per minute as far as cutting > goes for me. i have until october to attain this goal. this minute > for cutting is to include facing, writing out slides, cutting, and > putting tray into symphony stainer (not to mention getting up to > answer the phone, fielding questions regarding send-out cases, and other slight > "cutting interruptions). this seems an extreme, possibly unattainable > goal. i'm up for a challenge at age 53, but any advice would be > SWONDERFUL!!!! :) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From christiegowan <@t> msn.com Mon Jun 27 15:14:46 2011 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Mon Jun 27 15:14:49 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: <92AD9B20A6C38C4587A9FEBE3A30E164082116E941@CHEXCMS10.one.ads.che.org> References: AANLkTimSmJ6JO=uRvj_djA-3h5Q5gjf2p2fEEG5mjtnE@mail.gmail.com, <498DDA84FDA449B284C69014FBF60684@JoannePC>, , <92AD9B20A6C38C4587A9FEBE3A30E164082116E941@CHEXCMS10.one.ads.che.org> Message-ID: The proper way to do this would be to time each tech in your lab as to how many blocks they can cut in one hour. Take everyones number of blocks and do an average for your lab. The average for my lab is 2.15 minutes per block. I compiled these numbers in an effort to determine what our labor costs are, not to set an expectation for productivity. I have some people who cut really fast and some who cut slow. I am curious as to how your management came up with these numbers and are any of them histotechs?? Christie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne > Sent: Saturday, June 25, 2011 2:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] How many tissues an histo tech is suppose to cut per > > > > i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Mon Jun 27 15:42:29 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Mon Jun 27 15:42:34 2011 Subject: [Histonet] Austin PRN Message-ID: Hello all =) Since I just recently got married and still own a house we do not live in, I am finding myself in the pickle of 2 houses and not enough income...Does anyone know of any kind of part time histology jobs in Austin that I could go to either early until around 7am or late after 5pm? Or if not histology specific, I could log specimens for intake or something...I just can't bring myself to ask the question..."would you like fries with that?" with a college education and 10 years of histology experience...I'm doing ok, I just want my fluff back =) Thanks ya'll!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From member <@t> linkedin.com Mon Jun 27 16:44:39 2011 From: member <@t> linkedin.com (Thomas Huynh via LinkedIn) Date: Mon Jun 27 16:44:43 2011 Subject: [Histonet] Invitation to connect on LinkedIn Message-ID: <1642189695.6429738.1309211079898.JavaMail.app@ela4-bed34.prod> LinkedIn ------------ Thomas Huynh requested to add you as a connection on LinkedIn: ------------------------------------------ David, I'd like to add you to my professional network on LinkedIn. - Thomas Accept invitation from Thomas Huynh http://www.linkedin.com/e/yvpgd1-gpfyh2hc-1e/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I124609207_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYTc38Vc3oQcz59bQRLsDpOqkRVbP8QcjcOe3oRcPcLrCBxbOYWrSlI/EML_comm_afe/ View invitation from Thomas Huynh http://www.linkedin.com/e/yvpgd1-gpfyh2hc-1e/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I124609207_13/3cNnPsMczAMdzgOckALqnpPbOYWrSlI/svi/ ------------------------------------------ DID YOU KNOW LinkedIn can help you find the right service providers using recommendations from your trusted network? Using LinkedIn Services, you can take the risky guesswork out of selecting service providers by reading the recommendations of credible, trustworthy members of your network. http://www.linkedin.com/e/yvpgd1-gpfyh2hc-1e/svp/inv-25/ -- (c) 2011, LinkedIn Corporation From anonwums1 <@t> gmail.com Mon Jun 27 19:49:23 2011 From: anonwums1 <@t> gmail.com (Adam .) Date: Mon Jun 27 19:49:28 2011 Subject: [Histonet] Flk2 / Flt3 / CD135 on mouse bone Message-ID: Hi all, One of my fellow graduate students is trying to perform immunofluorescence on PFA fixed, EDTA decalcified mouse bone for Flk2 (aka Flt3 or CD135). So far, he hasn't had any luck on either paraffin embedded or frozen sections using HIER. Has anyone done this successfully? I know the ideal way to perform IF on bone is using a tape-transfer system, but, alas, we don't quite have that working yet. Thanks, Adam From koellingr <@t> comcast.net Mon Jun 27 20:43:48 2011 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Mon Jun 27 20:43:56 2011 Subject: [Histonet] Flk2 / Flt3 / CD135 on mouse bone In-Reply-To: Message-ID: <721308736.188871.1309225428159.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Adam/his fellow grad student, ? I could be?somewhat off not being up to date with current literature.? Sorry.? But used to look for Flk2/Flt3/CD135 in murine bone marrow and not the bone itself.? In immature hematopoeitic progenitor cells.? Along with looking in thymus, etc.? But for something like femurs, I removed the bone marrow, and there are some slick ways to do it without disrupting the core architecture too much.? Could take it out and obviously flow (cytometry) the cells.? But could also get out a fairly intact core and freeze it, or paraffin process/section it or glycol methacrylate section it and worry very little about the minute amount of trabecular bone in murine bone marrow. Depending on age, etc of course. ? So unless for the specific needs of the project call for looking actually for staining within the cortical bone, I'd take that problem out of the picture by getting the bone marrow out and then not?having to worry ?about decalcification and tape transfer and things like that.? Makes IHC staining a lot easier in my experience?but ?perhaps there are those out there who have worked out whole, intact mouse bone CD135 staining. Ray Ray Koelling PhenoPath Labs Seattle WA ----- Original Message ----- From: "Adam ." To: histonet@lists.utsouthwestern.edu Sent: Monday, June 27, 2011 5:49:23 PM Subject: [Histonet] Flk2 / Flt3 / CD135 on mouse bone Hi all, One of my fellow graduate students is trying to perform immunofluorescence on PFA fixed, EDTA decalcified mouse bone for Flk2 (aka Flt3 or CD135). So far, he hasn't had any luck on either paraffin embedded or frozen sections using HIER. Has anyone done this successfully? I know the ideal way to perform IF on bone is using a tape-transfer system, but, alas, we don't quite have that working yet. Thanks, Adam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anonwums1 <@t> gmail.com Mon Jun 27 20:52:09 2011 From: anonwums1 <@t> gmail.com (Adam .) Date: Mon Jun 27 20:52:14 2011 Subject: [Histonet] Flk2 / Flt3 / CD135 on mouse bone In-Reply-To: <721308736.188871.1309225428159.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> References: <721308736.188871.1309225428159.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: Hi Ray, That is a great idea. We are indeed looking for Flk2 expression on hematopoietic progenitors cells, but alas, we are looking at these cells in relationship to bone elements such as osteoblasts, so we need to do it on intact bone. Adam On Mon, Jun 27, 2011 at 8:43 PM, wrote: > Adam/his fellow grad student, > > > > I could be somewhat off not being up to date with current literature. > Sorry. But used to look for Flk2/Flt3/CD135 in murine bone marrow and not > the bone itself. In immature hematopoeitic progenitor cells. Along with > looking in thymus, etc. But for something like femurs, I *removed* the > bone marrow, and there are some slick ways to do it without disrupting the > core architecture too much. Could take it out and obviously flow > (cytometry) the cells. But could also get out a fairly intact core and > freeze it, or paraffin process/section it or glycol methacrylate section it > and worry very little about the minute amount of trabecular bone in murine > bone marrow. Depending on age, etc of course. > > > > So unless for the specific needs of the project call for looking actually > for staining within the cortical bone, I'd take that problem out of the > picture by getting the bone marrow out and then not having to worry about > decalcification and tape transfer and things like that. Makes IHC staining > a lot easier in my experience but perhaps there are those out there who > have worked out whole, intact mouse bone CD135 staining. > > > > Ray > > > > Ray Koelling > > PhenoPath Labs > > Seattle WA > > ------------------------------ > > *From: *"Adam ." > *To: *histonet@lists.utsouthwestern.edu > *Sent: *Monday, June 27, 2011 5:49:23 PM > *Subject: *[Histonet] Flk2 / Flt3 / CD135 on mouse bone > > > Hi all, > > One of my fellow graduate students is trying to perform immunofluorescence > on PFA fixed, EDTA decalcified mouse bone for Flk2 (aka Flt3 or CD135). So > far, he hasn't had any luck on either paraffin embedded or frozen sections > using HIER. Has anyone done this successfully? I know the ideal way to > perform IF on bone is using a tape-transfer system, but, alas, we don't > quite have that working yet. > > Thanks, > Adam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Susan.Walzer <@t> HCAHealthcare.com Tue Jun 28 02:21:03 2011 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Tue Jun 28 02:21:14 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: References: AANLkTimSmJ6JO=uRvj_djA-3h5Q5gjf2p2fEEG5mjtnE@mail.gmail.com<498DDA84FDA449B284C69014FBF60684@JoannePC> <5A2BD13465E061429D6455C8D6B40E390EBF65EA57@IBMB7Exchange.digestivespecialists.com> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2BAFA10CB0@FWDCWPMSGCMS09.hca.corpad.net> Would you ask a surgeon to speed up his procedure? We are also using sharp blades and cutting off the tip of a finger is a real outcome to speed cutting. What we do effects patient outcome and quality must always come first. Speed come gradually after. When you treat patient tissue on a assembly line you diminish the importance of what we do. The pathologist cannot diagnose disease without quality slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Monday, June 27, 2011 1:52 PM To: lblazek@digestivespecialists.com; joanne0658@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per I second this motion!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, June 27, 2011 12:10 PM To: 'Joanne'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per Unless you are in some remote area that there aren't any other facilities around, I would look for a new job! I don't think your age should have any bearing on finding one. If you were close to me I'd hire you. Working under that kind of condition is unacceptable in my opinion. It promotes errors and that isn't what we are all about. Those blocks are our patients. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Jun 28 08:48:55 2011 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Jun 28 08:48:59 2011 Subject: [Histonet] Re: Histogel In-Reply-To: References: Message-ID: I agree with the Samuri on histogel, it can be a great tool for making cell blocks but sometimes once I get the block to the microtome either for frozens or ffpe blocks it does not section well and can be a nightmare. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, June 27, 2011 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histogel Histogel is indeed expensive, and often not permitted by Management for that reason. Preparing your own agar is a bit tricky - you really need a hot plate and a magnetic stirrer, unlikely items in a histology lab. (2% or 3% dry agar, in water.) Back before hospital microbiology turned into the black box it is today, you used to be able to walk across the hall and pick up a tube of trypticase soy agar (TSA - I think it was 3% agar with some stuff in it to make bugs grow) and use that. One way to use agar is for the pathologist to pour out some melted agar on a glass slide or metal ruler, and embed small specimens in it so they stay oriented - works great for temporal artery biopsies and vasectomy specimens - time consuming. A blood bank heating block is useful for keeping tubes of agar melted at the gross desk. Whether you use Histogel or some other agar, it's very important not to commit any valuable specimens to it until you're sure it works in your system. I like to carve out some "pseudo-biopsies" from a normal mucosa in a colon resection specimen and run them. - I've seen some disasters when this precaution wasn't taken. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Tue Jun 28 09:32:22 2011 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Jun 28 09:32:32 2011 Subject: [Histonet] Educational Symposium in Columbus, OH Message-ID: For those of you interested in keeping up to date, please note there is an all-day Educational Symposium in Columbus, OH, on Thursday, July 14, 2011. Keynote Addresses in the morning: Standardization and Automation in Anatomic Pathology: How to meet the future needs for quality clinical care in a cost effective era" Syed K. Mohsin, MD Head of Breast Pathology & Medical Director, IHC Lab, Riverside Methodist/Grant Hospitals, Columbus, Ohio "When is a Diagnosis not a Diagnosis? When it belongs to the Wrong Patient!" Doyle Carney, Manager Workflow Solutions - Biosystems Division, Leica Microsystems, Buffalo Grove, IL Breakout sessions after lunch. For more information, please contact your Leica rep, or I can forward program if interested Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From sgoebel <@t> mirnarx.com Tue Jun 28 09:36:01 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Tue Jun 28 09:36:04 2011 Subject: [Histonet] PAS oops... Message-ID: So I dumped my periodic acid like a moron...does anyone know of another reagent that will oxidize for the PAS reaction? Looks like I have acetic and hydrochloric acids...and that is all...hmm... Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From liz <@t> premierlab.com Tue Jun 28 09:47:01 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Jun 28 09:47:05 2011 Subject: [Histonet] RE: PAS oops... In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE0646F6F8@SBS2K8.premierlab.local> Chromic acid will work but I think that's for PAS for fungus. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Tuesday, June 28, 2011 8:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS oops... So I dumped my periodic acid like a moron...does anyone know of another reagent that will oxidize for the PAS reaction? Looks like I have acetic and hydrochloric acids...and that is all...hmm... Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barbara.Crill <@t> LPNT.net Tue Jun 28 09:54:15 2011 From: Barbara.Crill <@t> LPNT.net (Barbara.Crill@LPNT.net) Date: Tue Jun 28 09:54:21 2011 Subject: [Histonet] VENTANA ULTRA & ER,PR,HER2 Message-ID: <7DA79EBDBD92BF408EF392413737878D3942992FAA@NADCWPMSGCMS01.hca.corpad.net> We are investigating getting the Ventana Ultra. I discovered that the ER, PR, & HER2 are not yet FDA approved. If you are using the Ventana Ultra how are you doing the ER, PR, & HER2? Do you use the Benchmark XT? Is anyone using the INFORM HER2 Dual ISH DNA Probe Cocktail Assay? ANTOINETTE CRILL, E-mail: barbara.crill@LPNT.net From bakevictoria <@t> gmail.com Tue Jun 28 09:54:59 2011 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Jun 28 09:55:03 2011 Subject: [Histonet] RE: PAS oops... In-Reply-To: <14E2C6176416974295479C64A11CB9AE0646F6F8@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE0646F6F8@SBS2K8.premierlab.local> Message-ID: I'm hoping that the attachment will attach. It's an old procedure from the 1950's Vikki If this does not attach, please let me know. http://jcs.biologists.org/content/s3-97/37/11.full.pdf On Tue, Jun 28, 2011 at 10:47 AM, Elizabeth Chlipala wrote: > Chromic acid will work but I think that's for PAS for fungus. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308-1592 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > sgoebel@mirnarx.com > Sent: Tuesday, June 28, 2011 8:36 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] PAS oops... > > So I dumped my periodic acid like a moron...does anyone know of another > reagent that will oxidize for the PAS reaction? Looks like I have > acetic and hydrochloric acids...and that is all...hmm... > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From algranth <@t> email.arizona.edu Tue Jun 28 10:05:43 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Jun 28 10:05:57 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2BAFA10CB0@FWDCWPMSGCMS09.hca.corpad.net> References: AANLkTimSmJ6JO=uRvj_djA-3h5Q5gjf2p2fEEG5mjtnE@mail.gmail.com<498DDA84FDA449B284C69014FBF60684@JoannePC> <5A2BD13465E061429D6455C8D6B40E390EBF65EA57@IBMB7Exchange.digestivespecialists.com> <4BF03F5404EBDE409AF9232DA74B9DED2BAFA10CB0@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: I have to jump into this discussion if only to say that I am in total agreement with Susan and others regarding quality over speed. Over the last few years I've had many students rotate through my lab - a research core facility - and when I'm teaching them to cut the perfect section they tell me that in the clinical labs they don't have time for perfect. It is sad that we can't all strive to be the best that we can be especially when the outcome of what we do has a huge impact on a patient's treatment in many cases. When I was "growing up" in histology I had a pathologist who impressed on me the importance of good sections. He said the job of the pathologist is hard enough without trying to read out slides that are less than optimal and this is what you get when you rush through the sectioning. Just try to cut one slide per minute and see what your pathologist has to say about the sections. Andi On Jun 28, 2011, at 12:21 AM, wrote: > Would you ask a surgeon to speed up his procedure? We are also using sharp blades and cutting off the tip of a finger is a real outcome to speed cutting. What we do effects patient outcome and quality must always come first. Speed come gradually after. When you treat patient tissue on a assembly line you diminish the importance of what we do. The pathologist cannot diagnose disease without quality slides. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com > Sent: Monday, June 27, 2011 1:52 PM > To: lblazek@digestivespecialists.com; joanne0658@comcast.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per > > I second this motion!! > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, > Linda > Sent: Monday, June 27, 2011 12:10 PM > To: 'Joanne'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut > per > > Unless you are in some remote area that there aren't any other > facilities around, I would look for a new job! I don't think your age > should have any bearing on finding one. If you were close to me I'd > hire you. Working under that kind of condition is unacceptable in my > opinion. It promotes errors and that isn't what we are all about. > Those blocks are our patients. > > Linda > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne > Sent: Saturday, June 25, 2011 2:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] How many tissues an histo tech is suppose to cut per > > > > i've only been working 2 months. although older, i am new as a > histotech (graduated in may 2010, found a job in april 2011). seems > management is setting a goal of a block per minute as far as cutting > goes for me. i have until october to attain this goal. this minute for > cutting is to include facing, writing out slides, cutting, and putting > tray into symphony stainer (not to mention getting up to answer the > phone, fielding questions regarding send-out cases, and other slight > "cutting interruptions). this seems an extreme, possibly unattainable > goal. i'm up for a challenge at age 53, but any advice would be > SWONDERFUL!!!! :) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sbaldwin <@t> mhhcc.org Tue Jun 28 10:09:39 2011 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Tue Jun 28 10:09:31 2011 Subject: [Histonet] Processing- Grossing Personnel requirement Message-ID: Hi Histonetters: We are Joint Commission inspected and I was wondering if anyone can shoot me an e-mail with the requirements from the CAP checklist for techs grossing. Any help would be greatly appreciated! Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-482-0210, 0216, Fax 812-482-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. From TNMayer <@t> mdanderson.org Tue Jun 28 10:18:52 2011 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue Jun 28 10:21:41 2011 Subject: [Histonet] RE: How many tissues an histo tech is suppose to cut per hour In-Reply-To: References: Message-ID: I have always heard that 30 blocks/hr is good for an entry level tech. If you can cut more, great, but being 'fresh' 30 blocks is fine. The quality is what is important. You can also go to the pathologist and show them some slides that are cut at the requested rate, and then those cut at your normal rate and ask them to pick the best. Getting them on your side is beneficial. If you cut too fast the quality can be shot and you may float the wrong tissue or give a crappy section. Hope this helps. Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Message: 1 Date: Mon, 27 Jun 2011 13:10:11 -0400 From: "Blazek, Linda" Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per To: 'Joanne' , "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E390EBF65EA57@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Unless you are in some remote area that there aren't any other facilities around, I would look for a new job! I don't think your age should have any bearing on finding one. If you were close to me I'd hire you. Working under that kind of condition is unacceptable in my opinion. It promotes errors and that isn't what we are all about. Those blocks are our patients. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ From rsrichmond <@t> gmail.com Tue Jun 28 10:24:30 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Jun 28 10:24:34 2011 Subject: [Histonet] Re: Oncotype Message-ID: Oncotype for breast cancer is approved for Medicare. Some competing breast cancer tests probably are also. Such services are rapidly proliferating for other malignant tumors, also. The important thing for your laboratory is to be very sure who's going to be paying for the test, somewhere around USD$3,000. This responsibility can sometimes be pushed off onto the oncologist's office. The usefulness of such tests is considerably controversial. Bob Richmond Samurai Pathologist Knoxville TN From JEllin <@t> yumaregional.org Tue Jun 28 10:35:07 2011 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Jun 28 10:35:30 2011 Subject: [Histonet] VENTANA ULTRA & ER,PR,HER2 In-Reply-To: <7DA79EBDBD92BF408EF392413737878D3942992FAA@NADCWPMSGCMS01.hca.corpad.net> References: <7DA79EBDBD92BF408EF392413737878D3942992FAA@NADCWPMSGCMS01.hca.corpad.net> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8035D6E7C@EXCHANGECLUSTER.yumaregional.local> We will be going through this transition, here at Yuma Regional Medical Center.. Call me if you have questions Jesus Ellin 928-336-1743 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Tuesday, June 28, 2011 7:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VENTANA ULTRA & ER,PR,HER2 We are investigating getting the Ventana Ultra. I discovered that the ER, PR, & HER2 are not yet FDA approved. If you are using the Ventana Ultra how are you doing the ER, PR, & HER2? Do you use the Benchmark XT? Is anyone using the INFORM HER2 Dual ISH DNA Probe Cocktail Assay? ANTOINETTE CRILL, E-mail: barbara.crill@LPNT.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From hrfulklab <@t> gmail.com Tue Jun 28 10:36:56 2011 From: hrfulklab <@t> gmail.com (Heather Rumbut) Date: Tue Jun 28 10:36:59 2011 Subject: [Histonet] CLIA requirements for Profiency Testing Message-ID: Getting ducks in a row for CLIA inspection, and saw proficiency testing on thier checklist. We are a dermatology inhouse lab, anyone doing PT testing in same situation? If so, who do you use? Thanks From algranth <@t> email.arizona.edu Tue Jun 28 10:53:06 2011 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Jun 28 10:53:32 2011 Subject: [Histonet] RE: How many tissues an histo tech is suppose to cut per hour In-Reply-To: References: Message-ID: <98EB214C-ACDE-4B69-8861-305382E2D74A@email.arizona.edu> Absolutely - get the pathologists on your side. They have the clout. USE them. 8-) On Jun 28, 2011, at 8:18 AM, Mayer,Toysha N wrote: > > I have always heard that 30 blocks/hr is good for an entry level tech. If you can cut more, great, but being 'fresh' 30 blocks is fine. The quality is what is important. You can also go to the pathologist and show them some slides that are cut at the requested rate, and then those cut at your normal rate and ask them to pick the best. Getting them on your side is beneficial. > If you cut too fast the quality can be shot and you may float the wrong tissue or give a crappy section. > > Hope this helps. > > > Toysha N. Mayer, MBA, HT (ASCP) > Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > Message: 1 > Date: Mon, 27 Jun 2011 13:10:11 -0400 > From: "Blazek, Linda" > Subject: RE: [Histonet] How many tissues an histo tech is suppose to > cut per > To: 'Joanne' , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <5A2BD13465E061429D6455C8D6B40E390EBF65EA57@IBMB7Exchange.digestivespecialists.com> > > Content-Type: text/plain; charset="us-ascii" > > Unless you are in some remote area that there aren't any other facilities around, I would look for a new job! I don't think your age should have any bearing on finding one. If you were close to me I'd hire you. Working under that kind of condition is unacceptable in my opinion. It promotes errors and that isn't what we are all about. Those blocks are our patients. > > Linda > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne > Sent: Saturday, June 25, 2011 2:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] How many tissues an histo tech is suppose to cut per > > > > i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PAMarcum <@t> uams.edu Tue Jun 28 11:03:50 2011 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Jun 28 11:03:56 2011 Subject: FW: [Histonet] RE: How many tissues an histo tech is suppose to cut per hour Message-ID: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Tuesday, June 28, 2011 10:53 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: How many tissues an histo tech is suppose to cut per hour Absolutely - get the pathologists on your side. They have the clout. USE them. 8-) On Jun 28, 2011, at 8:18 AM, Mayer,Toysha N wrote: > > I have always heard that 30 blocks/hr is good for an entry level tech. If you can cut more, great, but being 'fresh' 30 blocks is fine. The quality is what is important. You can also go to the pathologist and show them some slides that are cut at the requested rate, and then those cut at your normal rate and ask them to pick the best. Getting them on your side is beneficial. > If you cut too fast the quality can be shot and you may float the wrong tissue or give a crappy section. > > Hope this helps. > > > Toysha N. Mayer, MBA, HT (ASCP) > Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > Message: 1 > Date: Mon, 27 Jun 2011 13:10:11 -0400 > From: "Blazek, Linda" > Subject: RE: [Histonet] How many tissues an histo tech is suppose to > cut per > To: 'Joanne' , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <5A2BD13465E061429D6455C8D6B40E390EBF65EA57@IBMB7Exchange.digestivespecialists.com> > > Content-Type: text/plain; charset="us-ascii" > > Unless you are in some remote area that there aren't any other facilities around, I would look for a new job! I don't think your age should have any bearing on finding one. If you were close to me I'd hire you. Working under that kind of condition is unacceptable in my opinion. It promotes errors and that isn't what we are all about. Those blocks are our patients. > > Linda > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne > Sent: Saturday, June 25, 2011 2:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] How many tissues an histo tech is suppose to cut per > > > > i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight "cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL!!!! :) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. -------------- next part -------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Tue Jun 28 11:10:47 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Tue Jun 28 11:10:52 2011 Subject: [Histonet] CLIA requirements for Profiency Testing In-Reply-To: References: Message-ID: <1309277447.89023.YahooMailRC@web120601.mail.ne1.yahoo.com> Hi Heather - We use an outside?Dermatopathologist to review 5% of the cases every quarter. Yours, Dave ________________________________ From: Heather Rumbut To: histonet@lists.utsouthwestern.edu Sent: Tue, June 28, 2011 11:36:56 AM Subject: [Histonet] CLIA requirements for Profiency Testing Getting ducks in a row for CLIA inspection, and saw proficiency testing on thier checklist.? We are a dermatology inhouse lab, anyone doing PT testing in same situation? If so, who do you use? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Jun 28 11:34:48 2011 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jun 28 11:34:53 2011 Subject: [Histonet] Exciting Brand Spanking Shiny New Opportunities Exclusively through RELIA Solutions in Beautiful Southwestern US and the Gorgeous Pacific Northwest. Message-ID: <08827AC846EB4448BFE1ECA9C868B280@ownerf1abaad51> Hi Histonetters!! I hope everyone is having a terrific Tuesday. I am pretty chipper today because I have 2 really great opportunities to tell you about. What they have in common is that both positions are brand new labs where you will have the opportunity to set up and run your own lab and hire and manage a staff as volume increases. My clients offer very competitive salaries, benefits and relocation assistance. The 1st position is located in Portland, OR where a client of mine is setting up an IHC lab and needs an ASCP Certified Immunohistochemistry Tech. The 2nd position is located in Farmington, NM where my client is in need of an ASCP certified Histotech with experience in routine histology and specials. GI experience is a plus. I have more information to share on both of these positions so if you or anyone you know might be interested in either of these opportunities please let me know. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. Have a great day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From arvidsonkristen <@t> yahoo.com Tue Jun 28 12:24:31 2011 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Tue Jun 28 12:24:35 2011 Subject: [Histonet] Derm Slide Artifact Message-ID: <1309281871.74809.YahooMailClassic@web65712.mail.ac4.yahoo.com> The Paths are noticing a halo efftect around the keratinocytes.? This is something new.? It appears to be happening during staining.? We haven't changed anything and we are making sure that all the reagents are fresh and full.? Thoughts? ? Thanks, Kristen From histotalk <@t> yahoo.com Tue Jun 28 12:32:26 2011 From: histotalk <@t> yahoo.com (David Kemler) Date: Tue Jun 28 12:32:30 2011 Subject: [Histonet] Educational Symposium in Columbus, OH In-Reply-To: References: Message-ID: <1309282346.26157.YahooMailRC@web120609.mail.ne1.yahoo.com> Ronnie - I have printed out your email and will read it on my July 10th show. I would have mentioned it on this past Sunday's show had I known about it. Sorry. Well, in the future. . .(smile) Your, Dave ________________________________ From: "Houston, Ronald" To: "Histonet (histonet@lists.utsouthwestern.edu)" Sent: Tue, June 28, 2011 10:32:22 AM Subject: [Histonet] Educational Symposium in Columbus, OH For those of you interested in keeping up to date, please note there is an all-day Educational Symposium in Columbus, OH, on Thursday, July 14, 2011. Keynote Addresses in the morning: Standardization and Automation in Anatomic Pathology: How to meet the future needs for quality clinical care in a cost effective era" Syed K. Mohsin, MD Head of Breast Pathology & Medical Director, IHC Lab, Riverside Methodist/Grant Hospitals, Columbus, Ohio "When is a Diagnosis not a Diagnosis? When it belongs to the Wrong Patient!" Doyle Carney, Manager Workflow Solutions - Biosystems Division, Leica Microsystems, Buffalo Grove, IL Breakout sessions after lunch. For more information, please contact your Leica rep, or I can forward program if interested Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.White3 <@t> va.gov Tue Jun 28 12:45:58 2011 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Tue Jun 28 12:46:04 2011 Subject: [Histonet] Slides per minute Message-ID: <2B2ECF33934F5D4996D8BE03EFDF397608452CEA@VHAV09MSGA3.v09.med.va.gov> If it helps, at our hospital it states that when sectioning, you maintain a monthly average of 1 slide every 3 minutes properly prepared. Honestly we don't hold anyone to it. Our doc's would implode if not given the most perfect sections possible which does take some time. Quality over quantity any day J Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From sbreeden <@t> nmda.nmsu.edu Tue Jun 28 13:24:15 2011 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Jun 28 13:24:20 2011 Subject: [Histonet] New Methylene Blue Message-ID: <4D14F0FC9316DD41972D5F03C070908B051DF800@nmdamailsvr.nmda.ad.nmsu.edu> Okay - it's not for me, but I have offered to make up a new solution of NEW METHYLENE BLUE for our specimen prep area. I need a "recipe" for making an aqueous solution used to stain fluid preps from animals (urines, etc.). I've checked Lillie, Carson and Sheehan and Google. Can't seem to find an appropriate Formula. Help? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) From rjbuesa <@t> yahoo.com Tue Jun 28 14:32:29 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 28 14:32:33 2011 Subject: [Histonet] New Methylene Blue In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B051DF800@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1309289549.39292.YahooMailClassic@web65708.mail.ac4.yahoo.com> The only NEW methylene blue formula I know is the one by Higham (1945) and is prepared as follows: water ? 75 mL 95% ethanol ? 25 mL methylene blue ? 0.1 g citric acid ? 0.2 g To use for 3 to 10 minutesRen? J. --- On Tue, 6/28/11, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] New Methylene Blue To: histonet@lists.utsouthwestern.edu Date: Tuesday, June 28, 2011, 2:24 PM Okay - it's not for me, but I have offered to make up a new solution of NEW METHYLENE BLUE for our specimen prep area.? I need a "recipe" for making an aqueous solution used to stain fluid preps from animals (urines, etc.).? I've checked Lillie, Carson and Sheehan and Google. Can't seem to find an appropriate Formula.? Help? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM? 87102 505-383-9278 (Histology Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linnette.Grove <@t> leica-microsystems.com Tue Jun 28 14:36:39 2011 From: Linnette.Grove <@t> leica-microsystems.com (Linnette.Grove@leica-microsystems.com) Date: Tue Jun 28 14:36:48 2011 Subject: [Histonet] Grove, Linnette is out of the office. Message-ID: I will be out of the office starting 06/28/2011 and will not return until 07/05/2011. I will be on vacation with limited access to email and voice mail until Tuesday, July 5. Regards, Linnette ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From hctrupath <@t> att.net Tue Jun 28 14:52:39 2011 From: hctrupath <@t> att.net (Heather Cooper) Date: Tue Jun 28 14:52:44 2011 Subject: [Histonet] (no subject) Message-ID: <1309290759.82846.YahooMailRC@web80004.mail.sp1.yahoo.com> Does anyone have a good protocol for Giemsa?? I really need it From rjbuesa <@t> yahoo.com Tue Jun 28 14:58:03 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 28 14:58:06 2011 Subject: [Histonet] (no subject) In-Reply-To: <1309290759.82846.YahooMailRC@web80004.mail.sp1.yahoo.com> Message-ID: <1309291083.14111.YahooMailClassic@web65705.mail.ac4.yahoo.com> For tissues or smears? I can send you mine for tissues. Ren? J. --- On Tue, 6/28/11, Heather Cooper wrote: From: Heather Cooper Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Tuesday, June 28, 2011, 3:52 PM Does anyone have a good protocol for Giemsa?? I really need it _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Luis.Chiriboga <@t> nyumc.org Tue Jun 28 15:11:43 2011 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Tue Jun 28 15:12:10 2011 Subject: [Histonet] HIF1a query Message-ID: Hi Everyone Is anyone using HIF1alpha from neomarkers/labvision/thermoscientific (catalog ms-1164). I have been getting some unusual and inconsistent results and would like to hear from anyone else who has experienced any problems/issues with this antibody. I am working in both human and mouse tissues. TIA Luis Luis Chiriboga Ph.D OCS Experimental Pathology IHC Core Lab Bellevue Hospital Center Department of Pathology 4w27 (212) 562-4667 Luis.Chiriboga@nyumc.org ------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
================================= 
From rjbuesa <@t> yahoo.com  Tue Jun 28 15:14:11 2011
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Jun 28 15:14:15 2011
Subject: [Histonet] Giemsa for tissues
Message-ID: <1309292051.14200.YahooMailClassic@web65706.mail.ac4.yahoo.com>







Heather:
My article?with the procedure?is attached.
Please let me know if you have any questions.
Ren? J.

--- On Tue, 6/28/11, Heather Cooper  wrote:


From: Heather Cooper 
Subject: Giemsa>??????????????/
To: "Rene J Buesa" 
Date: Tuesday, June 28, 2011, 4:00 PM






For Tissues...





From: Rene J Buesa 
To: histonet@lists.utsouthwestern.edu; Heather Cooper 
Sent: Tue, June 28, 2011 1:58:03 PM
Subject: Re: [Histonet] (no subject)






For tissues or smears? I can send you mine for tissues.
Ren? J.

--- On Tue, 6/28/11, Heather Cooper  wrote:


From: Heather Cooper 
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, June 28, 2011, 3:52 PM


Does anyone have a good protocol for Giemsa?? I really need it

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From estellamireles <@t> gmail.com  Tue Jun 28 15:49:29 2011
From: estellamireles <@t> gmail.com (Stella Mireles)
Date: Tue Jun 28 15:49:33 2011
Subject: [Histonet] How many tissues an histo tech is suppose to cut per
In-Reply-To: <498DDA84FDA449B284C69014FBF60684@JoannePC>
References: <498DDA84FDA449B284C69014FBF60684@JoannePC>
Message-ID: 

Joanne is not alone.

The lab I work at has informed us that due to their own research, a single
histotech should be able to do 100 blocks an hour.
I'm not sure if they expect embedding, trimming, writing slides, sectioning
and manual staining in this goal.

I am a seasoned histotech, and have tried to speak to my lab manager and lab
supervisor, (both are med. techs and have very little insight into what is
involved in producing a high quality slide), but their goal remains the
same.

I have an idea:  I feel like printing some of your responses to Joanne
question and showing them that their goal is unattainable as well.

Any other suggestions.
From Timothy.Morken <@t> ucsfmedctr.org  Tue Jun 28 16:03:34 2011
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy)
Date: Tue Jun 28 16:03:49 2011
Subject: [Histonet] How many tissues an histo tech is suppose to cut
 per
In-Reply-To: 
References: <498DDA84FDA449B284C69014FBF60684@JoannePC>
	
Message-ID: 

" ...a single histotech should be able to do 100 blocks an hour "

First clarify what "do" means in this context. Then ask for a copy of their research and sources to see how they got an obviously ridiculous number.

Then ask Rene Busa (he's on this listserve) for copies of his published papers on the subject. 

Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles
Sent: Tuesday, June 28, 2011 1:49 PM
To: Joanne
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per

Joanne is not alone.

The lab I work at has informed us that due to their own research, a single
histotech should be able to do 100 blocks an hour.
I'm not sure if they expect embedding, trimming, writing slides, sectioning
and manual staining in this goal.

I am a seasoned histotech, and have tried to speak to my lab manager and lab
supervisor, (both are med. techs and have very little insight into what is
involved in producing a high quality slide), but their goal remains the
same.

I have an idea:  I feel like printing some of your responses to Joanne
question and showing them that their goal is unattainable as well.

Any other suggestions.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From liz <@t> premierlab.com  Tue Jun 28 16:14:37 2011
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Tue Jun 28 16:14:41 2011
Subject: [Histonet] How many tissues an histo tech is suppose to cut per
In-Reply-To: 
Message-ID: <14E2C6176416974295479C64A11CB9AE0646F707@SBS2K8.premierlab.local>

Stella

I would ask for a copy of the research and see where they came up with that number, it's not obtainable even if it was just to section one section of a  block that has already been trimmed in.  I worked at a Clinical CRO back in the late 80's and the goal was 10 blocks per hour (embed, section, stain, coverslip and label) and other tasks related to daily histology duties.  This was easy to achieve.

On a good day and I'm a pretty quick tech I can estimate that once I trim in the blocks and if I have only one section per block to cut. I can cut about 45-60 blocks in an hour.

I own a small research based histo lab that is very quality driven. I place no time frame on the techs only that the work needs to be completed in a timely manner.  Many of our projects are so specific that it may take us about 5 minutes just to trim in one block since we need to section to an exact area within a tissue (optic nerve head in mouse and rat eyes).  We have microscopes next to each microtome so we can view unstained slides to see where we are at in the block.  I would rather spend more time trimming in the block to the correct area than guess and end up having to recut the samples.

To put it in a nutshell it may take a bit more time to cut a quality section, but that time is well spent since recuts of poorly cut sections will only lead to additional work, increased turn around time and increased costs.

Just my two cents

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles
Sent: Tuesday, June 28, 2011 2:49 PM
To: Joanne
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per

Joanne is not alone.

The lab I work at has informed us that due to their own research, a single
histotech should be able to do 100 blocks an hour.
I'm not sure if they expect embedding, trimming, writing slides, sectioning
and manual staining in this goal.

I am a seasoned histotech, and have tried to speak to my lab manager and lab
supervisor, (both are med. techs and have very little insight into what is
involved in producing a high quality slide), but their goal remains the
same.

I have an idea:  I feel like printing some of your responses to Joanne
question and showing them that their goal is unattainable as well.

Any other suggestions.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From micro <@t> superlink.net  Tue Jun 28 16:22:22 2011
From: micro <@t> superlink.net (Markus F. Meyenhofer)
Date: Tue Jun 28 16:22:33 2011
Subject: [Histonet] How many tissues an histo tech is suppose to cut per
References: <498DDA84FDA449B284C69014FBF60684@JoannePC>
	
Message-ID: <9A9AE8ABCC77491891AA5A5A33A5967C@DJ4VDH31>

Maybe 100 sections from one block???
----- Original Message ----- 
From: "Stella Mireles" 
To: "Joanne" 
Cc: 
Sent: Tuesday, June 28, 2011 4:49 PM
Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per


> Joanne is not alone.
>
> The lab I work at has informed us that due to their own research, a single
> histotech should be able to do 100 blocks an hour.
> I'm not sure if they expect embedding, trimming, writing slides, 
> sectioning
> and manual staining in this goal.
>
> I am a seasoned histotech, and have tried to speak to my lab manager and 
> lab
> supervisor, (both are med. techs and have very little insight into what is
> involved in producing a high quality slide), but their goal remains the
> same.
>
> I have an idea:  I feel like printing some of your responses to Joanne
> question and showing them that their goal is unattainable as well.
>
> Any other suggestions.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From PAMarcum <@t> uams.edu  Tue Jun 28 16:27:17 2011
From: PAMarcum <@t> uams.edu (Marcum, Pamela A)
Date: Tue Jun 28 16:27:22 2011
Subject: [Histonet] How many tissues an histo tech is suppose to cut per
In-Reply-To: <9A9AE8ABCC77491891AA5A5A33A5967C@DJ4VDH31>
References: <498DDA84FDA449B284C69014FBF60684@JoannePC>
	
	<9A9AE8ABCC77491891AA5A5A33A5967C@DJ4VDH31>
Message-ID: 

Perhaps your med techs should try cutting 100 an hour with quality sections that a pathologist can read!!!  They are to accustom to high throughput testing with only pushing a button in some cases.  I know med tech that are excellent Histologist but they do the work too. 

Pam Marcum
UAMS

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Markus F. Meyenhofer
Sent: Tuesday, June 28, 2011 4:22 PM
To: Stella Mireles; Joanne
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per

Maybe 100 sections from one block???
----- Original Message ----- 
From: "Stella Mireles" 
To: "Joanne" 
Cc: 
Sent: Tuesday, June 28, 2011 4:49 PM
Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per


> Joanne is not alone.
>
> The lab I work at has informed us that due to their own research, a single
> histotech should be able to do 100 blocks an hour.
> I'm not sure if they expect embedding, trimming, writing slides, 
> sectioning
> and manual staining in this goal.
>
> I am a seasoned histotech, and have tried to speak to my lab manager and 
> lab
> supervisor, (both are med. techs and have very little insight into what is
> involved in producing a high quality slide), but their goal remains the
> same.
>
> I have an idea:  I feel like printing some of your responses to Joanne
> question and showing them that their goal is unattainable as well.
>
> Any other suggestions.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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From a.byrnes <@t> accelpath.com  Tue Jun 28 16:52:03 2011
From: a.byrnes <@t> accelpath.com (Andrew Byrnes)
Date: Tue Jun 28 16:52:12 2011
Subject: [Histonet] AccelPath Telepathology Services
Message-ID: 

Hi,

Does anyone know of a lab needing:

Diagnostic Services:

Primary interpretations
Sub-specialty consultations
Expert opinions or second opinion

All done using digital pathology!  

Please let me know.


Andrew Byrnes
AccelPath, LLC
M: 732-312-8008
www.AccelPath.com
a.byrnes@accelpath.com






From algranth <@t> email.arizona.edu  Tue Jun 28 17:20:41 2011
From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth))
Date: Tue Jun 28 17:20:56 2011
Subject: [Histonet] How many tissues an histo tech is suppose to cut per
In-Reply-To: 
References: <498DDA84FDA449B284C69014FBF60684@JoannePC>
	
Message-ID: <2B2F21C8-0BB1-4FC3-ADD8-91F2C313FD45@email.arizona.edu>

I'd do it - along with Rene's paper which was published and the Canadian paper that was referenced yesterday.

Ask these people - if they would want their child's tissue bx treated in this manner?

They have no clue as to what we do - you might suggest that you put together an inservice for the lab managers on histopathology. Not hard to do using a little power point, you can take your own pictures of the steps that the tissue samples go through. Emphasize that cutting something like a fallopian tube is different than cutting cervix or bone. They might be happy that you are interested enough in providing insight into histopathology. I have something like this that I use often when speaking to service groups and high school students in my community.

If they don't want to consider all that you have to do - look for another job!

I had a problem with the OR people one time so I did a presentation for them on the importance of fixation and I took them through all the steps from patient to slide to pathologist. They were floored because they never thought it was such an involved process, and my tissues were treated much better by the OR staff.

Andi



On Jun 28, 2011, at 1:49 PM, Stella Mireles wrote:

> Joanne is not alone.
> 
> The lab I work at has informed us that due to their own research, a single
> histotech should be able to do 100 blocks an hour.
> I'm not sure if they expect embedding, trimming, writing slides, sectioning
> and manual staining in this goal.
> 
> I am a seasoned histotech, and have tried to speak to my lab manager and lab
> supervisor, (both are med. techs and have very little insight into what is
> involved in producing a high quality slide), but their goal remains the
> same.
> 
> I have an idea:  I feel like printing some of your responses to Joanne
> question and showing them that their goal is unattainable as well.
> 
> Any other suggestions.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From CIngles <@t> uwhealth.org  Tue Jun 28 17:21:45 2011
From: CIngles <@t> uwhealth.org (Ingles Claire )
Date: Tue Jun 28 17:23:13 2011
Subject: [Histonet] How many tissues an histo tech is suppose to cut per
References: AANLkTimSmJ6JO=uRvj_djA-3h5Q5gjf2p2fEEG5mjtnE@mail.gmail.com<498DDA84FDA449B284C69014FBF60684@JoannePC><5A2BD13465E061429D6455C8D6B40E390EBF65EA57@IBMB7Exchange.digestivespecialists.com><4BF03F5404EBDE409AF9232DA74B9DED2BAFA10CB0@FWDCWPMSGCMS09.hca.corpad.net>
	
Message-ID: <064F1ACBAE8A78469AE2E41D533D87E505A712@UWHC-MAIL2.uwhis.hosp.wisc.edu>

 
I frankly would like to be able to not worry so much about TAT so the larger tissues can fix better! 
Claire

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Grantham, Andrea L - (algranth)
Sent: Tue 6/28/2011 10:05 AM
To: undisclosed-recipients
Cc: HISTONET
Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per



I have to jump into this discussion if only to say that I am in total agreement with Susan and others regarding quality over speed.
Over the last few years I've had many students rotate through my lab - a research core facility -  and when I'm teaching them to cut the perfect section they tell me that in the clinical labs they don't have time for perfect. It is sad that we can't all strive to be the best that we can be especially when the outcome of what we do has a huge impact on a patient's treatment in many cases. When I was "growing up" in histology I had a pathologist who impressed on me the importance of good sections. He said the job of the pathologist is hard enough without trying to read out slides that are less than optimal and this is what you get when you rush through the sectioning.
Just try to cut one slide per minute and see what your pathologist has to say about the sections.
Andi








From tjasper <@t> copc.net  Tue Jun 28 18:04:01 2011
From: tjasper <@t> copc.net (Thomas Jasper)
Date: Tue Jun 28 18:04:06 2011
Subject: [Histonet] How many tissues an histo tech is suppose to cut per
References: <498DDA84FDA449B284C69014FBF60684@JoannePC> 
	
Message-ID: <90354A475B420441B2A0396E5008D49692C039@copc-sbs.COPC.local>

Hi Stella,

I would be more than happy let you take this response to your lab
management.  First of all, just a bit of background - I have been a
histologist for over 26 years.  I have an Associate Degree in
Histotechnology and a Bachelor's Degree in Applied Science.  I am a
registered HT (1986) ASCP certified #12664.  I have worked in;
university and VA clinical settings, pharmaceutical research, major
medical and now independent clinical service.  I have been in
supervision for over 10 years and function as a working supervisor. I
have been responsible for Cytology, Autopsy and Transcription as well as
Histology.  I served for a number of years as a safety officer.  I have
significant experience with immunohistochemistry from manual kits, using
concentrated antibodies in multiple species application to running the
latest in automated IHC.

Having said that, the notion that a single histotech should be able to
cut 100 blocks an hour is sheer lunacy!  Expecting anyone to even
attempt such an unrealistic goal is dangerous, irresponsible and
ridiculous.  I seriously question "their own research".  I'm sure you
couldn't sell it in a deli as it sounds like nothing more than bad
baloney.  According to my calculations, that would be 1 block every 36
seconds...let that sink in.  You mention you are dealing with med techs.
These med techs apparently have no concept about the realities of
Histology.  I am going to assume this is the case and you (Stella)
obviously know better.  I will lay out the basic problems and hope you
are able to drive home the point.

~ Volume - 100 blocks per hour equals 1 block every 36 seconds...really?
Can you make change for a dollar in 36 seconds, find your car keys and
start your car?  Now do this over and over and over again, hour after
hour.  Even 50 blocks an hour is insane.

~ Variety - Histologists cut blocks from every part of the human body
(or animal or plant).  The specimens can be big or small, thick or thin,
hard or soft.  They can be dry and brittle, full of sutures and staples,
under-fixed and poorly processed.  When sectioning you are subject to
humidity, air currents, quality of the knife edge and specimen
orientation (and you just gave me a whole 36 seconds).

~ Quality - This is the number one consideration in my lab and any lab
worth its salt.  Quality is not achieved in one block every 36 seconds.
I just mentioned a list of variables and out of that a histologist has
to produce a microscopic work of art, one slide at a time, every time.
Any pathologist worth his or her salt will tell you that.  If you aren't
giving a good picture to that doctor, he or she is not going to be
happy.  You will want to figure in some additional time beyond 36
seconds for all the rework you're going to get.

~ Patient Care - Every histologist knows that a specimen/block/slide is
a patient.  That patient could be your mom, dad, sister, brother or some
other loved one and must be treated as such (regardless of who it is).
Trying to force histology work through at an impossible rate is
practicing bad medicine.  Is that how you would want your biopsy
handled?  If there is nothing more important than the patient, I think
the patient is worth more than 36 seconds.

~ Safety - Safety is easy to practice and easy to ignore.  What are we
dealing with here...extremely sharp blades for one.  The occasional
histologist may be known to skirt a safety rule now and then.  Don't get
your fingers too close to the blade.  With automated microtomes there
are new and exciting technical features to consider from a safety
perspective.  Regardless of the situation, speed factors into safety.
Existing stress factors combined with new ones for unrealistic speed is
an accident waiting to happen.  And there are other mental health
considerations from undue stress.

~ Special Testing - Not only are quality sections required for standard
hematoxylin and eosin staining.  Quality sections are required for
straight chemical, special staining, immunohistochemistry and other
special procedure applications labs may run.  For example, some
pre-treatments or other protocol steps involved in IHC may be a bit
harsh.  To rush and produce less than desirable sections for any of
these various procedures, due to unrealistic quotas is a bad idea.  Once
again all of the above apply to special testing.

Well Stella, I don't want to write a book and I'm sure I've left out
some valuable information.  I used to have unionized techs working for
me at my previous position.  I don't know if that's the case for you.  I
can guarantee the union steward would've had a field day with this one.
Also, this is the type of thing that OSHA loves to get wind of, along
with any state agency that regulates labor.  Please contact me if you'd
like to speak about this further.  Not to sound extreme, but there are
other jobs and nice, reasonable people to work for.

Kind regards,
Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjasper@copc.net   

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella
Mireles
Sent: Tuesday, June 28, 2011 1:49 PM
To: Joanne
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut
per

Joanne is not alone.

The lab I work at has informed us that due to their own research, a
single
histotech should be able to do 100 blocks an hour.
I'm not sure if they expect embedding, trimming, writing slides,
sectioning
and manual staining in this goal.

I am a seasoned histotech, and have tried to speak to my lab manager and
lab
supervisor, (both are med. techs and have very little insight into what
is
involved in producing a high quality slide), but their goal remains the
same.

I have an idea:  I feel like printing some of your responses to Joanne
question and showing them that their goal is unattainable as well.

Any other suggestions.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From histotech <@t> imagesbyhopper.com  Tue Jun 28 18:11:37 2011
From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com)
Date: Tue Jun 28 18:11:46 2011
Subject: [Histonet] How many tissues an histo tech is suppose to cut per
In-Reply-To: <2B2F21C8-0BB1-4FC3-ADD8-91F2C313FD45@email.arizona.edu>
References: <498DDA84FDA449B284C69014FBF60684@JoannePC>
	
	<2B2F21C8-0BB1-4FC3-ADD8-91F2C313FD45@email.arizona.edu>
Message-ID: <0F5FE2E1-4ED9-488F-AB97-354963924172@imagesbyhopper.com>

OMG, 100 blocks/hour?  Seriously?  I am fast , but even I can't touch that!  That's simply insane!!  :o(



Sent from my iPhone

On Jun 28, 2011, at 6:20 PM, "Grantham, Andrea L - (algranth)"  wrote:

> I'd do it - along with Rene's paper which was published and the Canadian paper that was referenced yesterday.
> 
> Ask these people - if they would want their child's tissue bx treated in this manner?
> 
> They have no clue as to what we do - you might suggest that you put together an inservice for the lab managers on histopathology. Not hard to do using a little power point, you can take your own pictures of the steps that the tissue samples go through. Emphasize that cutting something like a fallopian tube is different than cutting cervix or bone. They might be happy that you are interested enough in providing insight into histopathology. I have something like this that I use often when speaking to service groups and high school students in my community.
> 
> If they don't want to consider all that you have to do - look for another job!
> 
> I had a problem with the OR people one time so I did a presentation for them on the importance of fixation and I took them through all the steps from patient to slide to pathologist. They were floored because they never thought it was such an involved process, and my tissues were treated much better by the OR staff.
> 
> Andi
> 
> 
> 
> On Jun 28, 2011, at 1:49 PM, Stella Mireles wrote:
> 
>> Joanne is not alone.
>> 
>> The lab I work at has informed us that due to their own research, a single
>> histotech should be able to do 100 blocks an hour.
>> I'm not sure if they expect embedding, trimming, writing slides, sectioning
>> and manual staining in this goal.
>> 
>> I am a seasoned histotech, and have tried to speak to my lab manager and lab
>> supervisor, (both are med. techs and have very little insight into what is
>> involved in producing a high quality slide), but their goal remains the
>> same.
>> 
>> I have an idea:  I feel like printing some of your responses to Joanne
>> question and showing them that their goal is unattainable as well.
>> 
>> Any other suggestions.
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Delossantos_Roseann <@t> Allergan.com  Tue Jun 28 18:34:41 2011
From: Delossantos_Roseann <@t> Allergan.com (Delossantos_Roseann)
Date: Tue Jun 28 18:34:50 2011
Subject: [Histonet] How many tissues an histo tech is suppose to cut per
Message-ID: <6E159393AFE18142A83CE7C2475AD96D0928FCE329@AGMAILCL100.allergan.com>

Hi,
I've worked in a histology contract service lab  where quality and quantity was our bottom line, and I worked on various tissues and large volume of blocks at a time with very short deadlines.  100 blocks just sectioning one slide each in one hour is ridiculous especially if you consider the tissue type, quality of processed tissue, and if you have to locate a specific area of interest.  Moving the block in and out of the cryostat/microtome and other tasks like trimming the edges and labeling slides takes time as well.  100 sections from one block with a tissue that is very nice and cooperative is possible in one hour, especially if you are dealing with paraffin ribbons.  Since we sold our slides, quality trumps quantity every time and even in research where I used to and currently work in, quality is still the goal.  Histology is an art.


Rose

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From jtaramona70 <@t> gmail.com Tue Jun 28 19:16:15 2011 From: jtaramona70 <@t> gmail.com (Jose Taramona) Date: Tue Jun 28 19:16:26 2011 Subject: [Histonet] Histotech PM shift open at Lewisville Texas Message-ID: <877869F5-710C-4A84-A0DE-FA5D6E865E4D@gmail.com> Pathologist Bio-Medical Laboratories Looking for a positive energetic histotech for PM shift of 2-10 pm Must have experience with: Ventana immunohistochemistry system Kidney bx Need position filled immediately. For more information Send resume jtaramona@pbmlabs.com Jose Taramona Histology Supervisor From estellamireles <@t> gmail.com Tue Jun 28 21:21:18 2011 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Tue Jun 28 21:21:23 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: <6E159393AFE18142A83CE7C2475AD96D0928FCE329@AGMAILCL100.allergan.com> References: <6E159393AFE18142A83CE7C2475AD96D0928FCE329@AGMAILCL100.allergan.com> Message-ID: To All I Really Appreciate All The Great Info. I am fully armed and ready to present my case to management. The knowledge among everyone out there is incredible. Thanks. Stella From anonwums1 <@t> gmail.com Tue Jun 28 22:13:06 2011 From: anonwums1 <@t> gmail.com (Adam .) Date: Tue Jun 28 22:13:10 2011 Subject: [Histonet] Sad news Message-ID: Dear histology friends, I know this is a fairly close community, so I thought I should inform you that our local histotech, Patricia "Pat" Keller, passed away a few weeks ago, suddenly and unexpectedly. I apologize for the delay in relaying this information, but I wanted to wait until I confirmed the news with a coworker. From what I've heard secondhand, she came home from work one day, told her son she wasn't feeling well, went to sleep, and never woke up. I didn't know Pat that well, but I did spend one afternoon with her as she taught me to cut sections of mouse bone. In terms of the technical expertise she provided to the scientific community, she will definitely be missed. For those of you who knew her personally, I'm sure she will be missed much more. Adam From Melissa.Kuhnla <@t> chsli.org Wed Jun 29 04:38:37 2011 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Wed Jun 29 04:44:00 2011 Subject: [Histonet] VENTANA ULTRA & ER,PR,HER2 In-Reply-To: <7DA79EBDBD92BF408EF392413737878D3942992FAA@NADCWPMSGCMS01.hca.corpad.net> References: <7DA79EBDBD92BF408EF392413737878D3942992FAA@NADCWPMSGCMS01.hca.corpad.net> Message-ID: Hi Antoinette, We currently use Ventana's ER and PR on both XT and Ultra. From what I understand, the antibody dispenser is labeled IVD. We report all cases as being 'approved by the FDA for IVD use'. We do perform correlation/precision studies twice a year. For this study we run five cases on both the XT and an ultra and document that the result comes out the same. I have actually found that this is a compliance issue with Joint Commission. I can try to find the reference if you need it. It states that if you are running the same test on two different platforms, you must prove correlation. As far as the Her2 dual ISH, we are in the process of evaluating it now. I see it as a definite advantage that it is bright field, permanent, and has the potential to dramatically increase TAT. We are in the middle of a cost analysis. On the other hand, we currently run Pathvysion and I see that some literature calls FISH and pathvysion the 'gold standard'. This is the most highly scrutinized test we perform considering the CAP/ASCO guidelines. My gut also tells me...why take a chance with this when our current system is not 'broken'? I did find that CAP offers two mailings for profiency testing using bright field ISH. Let me know how you make out. Hope this helps. Melissa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara.Crill@LPNT.net Sent: Tuesday, June 28, 2011 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VENTANA ULTRA & ER,PR,HER2 We are investigating getting the Ventana Ultra. I discovered that the ER, PR, & HER2 are not yet FDA approved. If you are using the Ventana Ultra how are you doing the ER, PR, & HER2? Do you use the Benchmark XT? Is anyone using the INFORM HER2 Dual ISH DNA Probe Cocktail Assay? ANTOINETTE CRILL, E-mail: barbara.crill@LPNT.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From Dolores_Fischer <@t> baxter.com Wed Jun 29 07:50:40 2011 From: Dolores_Fischer <@t> baxter.com (Fischer, Dolores) Date: Wed Jun 29 07:51:19 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: References: <6E159393AFE18142A83CE7C2475AD96D0928FCE329@AGMAILCL100.allergan.com> Message-ID: Stella, Please keep us informed as to the outcome of your discussions with management. I hope it all works out in your favor. I have also been in the field over 35 yrs. and have never been known as one of the fastest sectioners, but I always focused on quality and thought about the patient behind the specimen. As we all know there are more skills to being a great technician than just the ability to section fast. I used to love doing special stains when I worked in clinical and my slides reflected that. I think we can all agree that working with a well rounded, conscientious tech who may not be the quickest in the field, but is a great team member, is a much more desired and valuable employee than someone who can section 60 on up blocks/hr. yet is not a very good "team player". I once went from working in research to taking a position in a small hospital that employed two techs. Both had left, so I walked into an empty lab ( they got by by having someone come in at night) and had to do everything myself including cyto preps. I'm sure I never managed 60 blks/hr but I got all the surgical done in a reasonable time. So good luck, and keep your options open, you may still want to look around for a position where the management understands histology. Seems there are lots of those places from all of the responses. Now about retiring to New Mexico............or that histo retirement community..........count me in! Dolores -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Tuesday, June 28, 2011 9:21 PM To: Delossantos_Roseann Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per To All I Really Appreciate All The Great Info. I am fully armed and ready to present my case to management. The knowledge among everyone out there is incredible. Thanks. Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From candice_camille <@t> yahoo.com Wed Jun 29 10:20:59 2011 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Wed Jun 29 10:21:03 2011 Subject: [Histonet] Quenching Message-ID: <1309360859.82237.YahooMailRC@web125416.mail.ne1.yahoo.com> Hi there my fellow histonetters! I was wondering if anyone out there can tell me the purpose of quenching? Thanks ?I remain yours truely, Candice Camille From rjbuesa <@t> yahoo.com Wed Jun 29 10:42:34 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 29 10:42:39 2011 Subject: [Histonet] Quenching In-Reply-To: <1309360859.82237.YahooMailRC@web125416.mail.ne1.yahoo.com> Message-ID: <1309362154.42040.YahooMailClassic@web65716.mail.ac4.yahoo.com> Candice: Quenching is usually done in IHC (immuno-histo-chemical) procedures and is intended to eliminate the?reaction of the peroxidase (enzyme) present in all cells. If this step is not done the background (noise) in the form of dark color present in the final section could mask the actual IHC reaction. Go to the DAKO website and download a free IHC manual that can explain you this and any other aspect of the IHC general procedure. Ren? J. --- On Wed, 6/29/11, Candice Smoots wrote: From: Candice Smoots Subject: [Histonet] Quenching To: "Histonet" Date: Wednesday, June 29, 2011, 11:20 AM Hi there my fellow histonetters! I was wondering if anyone out there can tell me the purpose of quenching? Thanks ?I remain yours truely, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Saro.Bascaramurty <@t> nrc-cnrc.gc.ca Wed Jun 29 11:57:05 2011 From: Saro.Bascaramurty <@t> nrc-cnrc.gc.ca (Bascaramurty, Saro) Date: Wed Jun 29 11:57:13 2011 Subject: [Histonet] Could you please recommend? Message-ID: Hi, I am in a research lab and currently we are looking into buying a good cryostat to work with human samples. I have always used a Leica model and currently we own a Leica 3050S. We would like get one that comes with decontamination feature (UV lamp etc...). I would like to know your positive and negative experiences with the models that you own. Which ones we should stay away from and which ones that you did not regret that you purchased it. Also some pricing details would be helpful. There were some earlier postings on this topic earlier which I did not bother to save. Thank you all in advance for your anticipated feedback. Saro Bascaramurty Technical Officer Institute for Biodiagnostics National Research Council 435 Ellice Avenue, Winnipeg, Manitoba. R3B 1Y6 Tel: 204-984-7166 Fax:204-984-6978 email:saro.bascaramurty@nrc-cnrc.gc.ca From Lisa.White3 <@t> va.gov Wed Jun 29 12:14:38 2011 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Wed Jun 29 12:14:44 2011 Subject: [Histonet] Could you please recommend? (Bascaramurty, Saro) Message-ID: <2B2ECF33934F5D4996D8BE03EFDF3976084531FE@VHAV09MSGA3.v09.med.va.gov> We use the Leica CM1850 UV. It is easy to use for sectioning as well as UV decontamination. Pricing fell in line with other vendors. I have used Shandon, Leitz, Leica and a unit so old I think it came with Noah on the boat don't even remember the maker. Leica is the favorite. It sections well and was the first one that the anti-roll plate would work correctly. It decontaminates well. No regrets, love it and would recommend. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From histotech <@t> imagesbyhopper.com Wed Jun 29 12:26:35 2011 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Jun 29 12:27:06 2011 Subject: [Histonet] Could you please recommend? (Bascaramurty, Saro) In-Reply-To: <2B2ECF33934F5D4996D8BE03EFDF3976084531FE@VHAV09MSGA3.v09.med.va.gov> References: <2B2ECF33934F5D4996D8BE03EFDF3976084531FE@VHAV09MSGA3.v09.med.va.gov> Message-ID: <833573BC-E95E-445F-9CD4-C414BE49399C@imagesbyhopper.com> I second the Leica! :o) Michelle Sent from my iPhone On Jun 29, 2011, at 1:14 PM, "White, Lisa M." wrote: > We use the Leica CM1850 UV. It is easy to use for sectioning as well as > UV decontamination. Pricing fell in line with other vendors. I have > used Shandon, Leitz, Leica and a unit so old I think it came with Noah > on the boat don't even remember the maker. Leica is the favorite. It > sections well and was the first one that the anti-roll plate would work > correctly. It decontaminates well. No regrets, love it and would > recommend. > > > > Lisa White, HT(ASCP) > > Supervisory HT > > James H. Quillen VAMC > > PO Box 4000 > > Corner of Veterans Way and Lamont > > PLMS 113 > > Mountain Home, TN 37684 > > 423-979-3567 > > 423-979-3401 fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Mandy.Bell <@t> chomp.org Wed Jun 29 12:26:52 2011 From: Mandy.Bell <@t> chomp.org (Bell, Mandy) Date: Wed Jun 29 12:28:22 2011 Subject: [Histonet] microtome blade donations Message-ID: Hi, One of our pathologists is going on a volunteer mission to a pathology lab in Malawi, Africa in a few weeks, and he is looking for donations of low profile microtome blades for the histology lab there. He said they will need around 200 blades or so, but any amount would help. He leaves on July 21st, so we are under a bit of a time crunch. Thanks! Mandy M Bell , HTL (ASCP) Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail From MLunetta <@t> luhcares.org Wed Jun 29 13:31:12 2011 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Wed Jun 29 13:31:32 2011 Subject: [Histonet] Billing for CD3/20 Message-ID: <4E0B1B10020000A800060303@ns.luhcares.org> Hey all, We are looking at doing the double stain CD3/20 and wanted to know how everyone is billing for it. Can we bill for 2 immuno stains? Or do we have to bill for one? What is the proper CPT for doing double or even triple staining? We are a Medicare facility. Thanks Matt Lunetta BS HT(ASCP) Longmont United Hospital Longmont, Colorado From gu.lang <@t> gmx.at Wed Jun 29 12:33:48 2011 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jun 29 13:33:51 2011 Subject: AW: [Histonet] Quenching In-Reply-To: <1309360859.82237.YahooMailRC@web125416.mail.ne1.yahoo.com> References: <1309360859.82237.YahooMailRC@web125416.mail.ne1.yahoo.com> Message-ID: <557E4085557D4BFA8F2974DB826A1161@dielangs.at> Quenching is also a term used with depressing fluorescence activity. This can happen in quantitative realtime-PCR procedures. Here a quencher is situated close to a fluorochrom and therefore the activity is low. During incorporation of the nucleotides the quencher is released and the fluorochrom-activity is restored. Gudrun Lang -----Ursprüngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Candice Smoots Gesendet: Mittwoch, 29. Juni 2011 17:21 An: Histonet Betreff: [Histonet] Quenching Hi there my fellow histonetters! I was wondering if anyone out there can tell me the purpose of quenching? Thanks  I remain yours truely, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Christina.Wilson <@t> leica-microsystems.com Wed Jun 29 14:19:27 2011 From: Christina.Wilson <@t> leica-microsystems.com (Christina.Wilson@leica-microsystems.com) Date: Wed Jun 29 14:19:02 2011 Subject: [Histonet] AUTO: is out of the office. (returning Mon 07/11/2011) Message-ID: I am out of the office from Wed 06/29/2011 until Mon 07/11/2011. I will have limited access to emails during this time. If you should need assistance, please contact Demaris Mills, demaris.mills@leica-microsystems.com, for product management support or Karen Niewerth, karen.niewerth@leica-microsystems.com, for customer service support. Note: This is an automated response to your message "Histonet Digest, Vol 91, Issue 41" sent on 6/29/2011 11:55:41 AM. This is the only notification you will receive while this person is away. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From nicole <@t> dlcjax.com Wed Jun 29 14:23:11 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed Jun 29 14:24:25 2011 Subject: [Histonet] Could you please recommend? (Bascaramurty, Saro) In-Reply-To: <833573BC-E95E-445F-9CD4-C414BE49399C@imagesbyhopper.com> References: <2B2ECF33934F5D4996D8BE03EFDF3976084531FE@VHAV09MSGA3.v09.med.va.gov> <833573BC-E95E-445F-9CD4-C414BE49399C@imagesbyhopper.com> Message-ID: <1794.208.62.167.196.1309375391.squirrel@webmail.realpages.com> Not sure about the UV thingy but I love the Leica 1850 or the 1510 works great. But, if I had to choose between the two I would go with the Leica 1850.. You can purchased a used cryo for about 12-15,000. Most times with a one year warrenty. I recommend calling Margaret at IMEB for a quote. She is wonderful and will find what you need. Nicole Tatum HT(ASCP) I second the Leica! :o) > > Michelle > > Sent from my iPhone > > On Jun 29, 2011, at 1:14 PM, "White, Lisa M." wrote: > >> We use the Leica CM1850 UV. It is easy to use for sectioning as well as >> UV decontamination. Pricing fell in line with other vendors. I have >> used Shandon, Leitz, Leica and a unit so old I think it came with Noah >> on the boat don't even remember the maker. Leica is the favorite. It >> sections well and was the first one that the anti-roll plate would work >> correctly. It decontaminates well. No regrets, love it and would >> recommend. >> >> >> >> Lisa White, HT(ASCP) >> >> Supervisory HT >> >> James H. Quillen VAMC >> >> PO Box 4000 >> >> Corner of Veterans Way and Lamont >> >> PLMS 113 >> >> Mountain Home, TN 37684 >> >> 423-979-3567 >> >> 423-979-3401 fax >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From nicole <@t> dlcjax.com Wed Jun 29 14:25:14 2011 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Wed Jun 29 14:25:59 2011 Subject: [Histonet] microtome blade donations In-Reply-To: References: Message-ID: <1802.208.62.167.196.1309375514.squirrel@webmail.realpages.com> Do they take volunteer histos, :) I would love to do somethig like that. Nicole Hi, > One of our pathologists is going on a volunteer mission to a pathology lab > in Malawi, Africa in a few weeks, and he is looking for donations of low > profile microtome blades for the histology lab there. He said they will > need around 200 blades or so, but any amount would help. He leaves on > July 21st, so we are under a bit of a time crunch. Thanks! > > Mandy M Bell , HTL (ASCP) > Histology Department > Community Hospital of the Monterey Peninsula > 831.625.4791 > > > P Please consider the environment before printing this e-mail > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mw <@t> personifysearch.com Wed Jun 29 14:26:20 2011 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed Jun 29 14:26:28 2011 Subject: [Histonet] New Position Alert - Field Histology Tech Southern CA Message-ID: <0e5375897002419969f6063ac902ea06@mail.gmail.com> Good Afternoon Histonet, One of our retained Cancer Diagnostics clients has opened up an Field Technical Support Opportunity based in Southern CA. This position is perfect for a Histotech looking to get out of the lab and into the field. Field Support Specialist - IHC The primary responsibility of this role will be to provide in-field technical applications support for current and next generation range of products. The FSS will install/set-up instrumentation in customer laboratories, perform demonstrations and maintain demonstration equipment in a clean and operational manner. The FSS will also train customers on the use of instrumentation. Conducting in-field post purchase applications support and performing instrument validations will be a key responsibility of this position. Position offers: Competitive Base Salary + Bonus/Commission + $700/Month for a Car Allowance and Gas Card, Cell Phone, Laptop, 401k, Full Benefits Open due to Promotion!! Please send your resumes to mw@personifysearch.com, or call me at 800.875.6188 ext. 103 Also, if you know of anyone who may be interested please do not hesitate to forward this e-mail, and have them reach out to me. Have a great day! Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From LSetlak <@t> childrensmemorial.org Wed Jun 29 15:33:55 2011 From: LSetlak <@t> childrensmemorial.org (Setlak, Lisa) Date: Wed Jun 29 15:36:45 2011 Subject: [Histonet] FW: The results of your email commands Message-ID: <7111DB39D045004C9CF29E79C71B28BC10212E00D3@CMHEXCC01MBX.childrensmemorial.org> Hi Histoland, Just wanted to give a quick update to a post I replied to a few weeks ago regarding floater contamination and it's relation to the Peloris processor. We've worked with Leica (thanks Cindy) and to reiterate what I stated in my response our source of contamination was most likely the grossing station or embedding center. Lisa From collette2 <@t> llnl.gov Wed Jun 29 16:06:01 2011 From: collette2 <@t> llnl.gov (Collette, Nicole M.) Date: Wed Jun 29 16:06:41 2011 Subject: [Histonet] Plastic processing for undecalcified mouse bones In-Reply-To: Message-ID: Hello, All, I am looking into starting a plastic embedding/sectioning endeavor, and am trying to figure out my startup reagents and protocols. I am seeing a lot of protocols for the Technovit system, which uses a slide press to adhere the sections to the slides, and would prefer to avoid expensive startup equipment if possible. I found another protocol that uses silanized slides, water and heat to adhere the sections. Is this comparable to results seen with a slide press, or is there some obvious down-side? I have been mainly looking into MMA/PMMA protocols for my bone, and ideally would like to be able to use the sections for antibody stains in addition to histology stains and mineral assessment- although I will work with the limitations of the medium, I know I can?t always ask for the moon. I will be using adult mouse bones, primarily from appendicular skeleton (long bones). I am trying to start with a relatively do-it-yourself, low throughput option that minimizes startup cost for a system that I may only use short-term. Up until now I have been using either paraffin embedding (decalcified samples), or frozen Cryojane sections (unfixed, undecalcified), but there is potential for plastic to be the best option in some instances. I think I have the sectioning capabilities covered, but would be appreciative of embedding and sectioning protocols (and sage advice from the wise, experienced bone cutters out there, if I?m totally headed in the wrong direction!). A catalog# recommendation for molds/chucks/cassettes (to fit or otherwise adapt to a Leica RM2255 microtome) would be fantastic. Thanks in advance for your help and support. Sincerely, Nicole Collette Lawrence Livermore National Lab collette2@llnl.gov From amosbrooks <@t> gmail.com Wed Jun 29 19:11:30 2011 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Jun 29 19:11:34 2011 Subject: [Histonet] Could you please recommend? Cryostats. Message-ID: Hi, I am also in research, and as such I am at the bottom of the barrel when it comes to NEW equipment. (Yes even lower than normal histo labs, think on that!) I congratulate you on being able to wrangle new equipment out of management. Due to this I can't recommend specific equipment, but I can recommend specific features. I have used cryostats clinically as well as in research, and one thing I noticed absent from most cryostats is the ability to adjust your block. For the life of me I cannot conceive of a reason why a company would have the audacity to sell a cryostat that does not allow the block to tilt. If I was in the market for a cryostat, I would not even consider one without this feature. Good Hunting, Amos On Wed, Jun 29, 2011 at 1:02 PM, wrote: > Message: 8 > Date: Wed, 29 Jun 2011 12:57:05 -0400 > From: "Bascaramurty, Saro" > Subject: [Histonet] Could you please recommend? > To: 'Histonet' > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hi, > > I am in a research lab and currently we are looking into buying a good > cryostat to work with human samples. I have always used a Leica model and > currently we own a Leica 3050S. We would like get one that comes with > decontamination feature (UV lamp etc...). I would like to know your positive > and negative experiences with the models that you own. Which ones we should > stay away from and which ones that you did not regret that you purchased it. > Also some pricing details would be helpful. > > There were some earlier postings on this topic earlier which I did not > bother to save. > > Thank you all in advance for your anticipated feedback. > > > Saro Bascaramurty > > Technical Officer > Institute for Biodiagnostics > National Research Council > 435 Ellice Avenue, > Winnipeg, Manitoba. R3B 1Y6 > > Tel: 204-984-7166 > Fax:204-984-6978 > email:saro.bascaramurty@nrc-cnrc.gc.ca > From amitapandey <@t> torrentpharma.com Thu Jun 30 00:01:52 2011 From: amitapandey <@t> torrentpharma.com (amitapandey@torrentpharma.com) Date: Thu Jun 30 00:02:14 2011 Subject: [Histonet] Could you please recommend? In-Reply-To: References: Message-ID: I have in my lab crytome FSE from shadon and it is working pretty well since last 5 years without any complain. Though i haven't experienced personally, but i heard this is better than leica one. also it has fumigation feature. Amita From: "Bascaramurty, Saro" To: 'Histonet' Date: 29/06/11 10:31 PM Subject: [Histonet] Could you please recommend? Sent by: histonet-bounces@lists.utsouthwestern.edu Hi, I am in a research lab and currently we are looking into buying a good cryostat to work with human samples. I have always used a Leica model and currently we own a Leica 3050S. We would like get one that comes with decontamination feature (UV lamp etc...). I would like to know your positive and negative experiences with the models that you own. Which ones we should stay away from and which ones that you did not regret that you purchased it. Also some pricing details would be helpful. There were some earlier postings on this topic earlier which I did not bother to save. Thank you all in advance for your anticipated feedback. Saro Bascaramurty Technical Officer Institute for Biodiagnostics National Research Council 435 Ellice Avenue, Winnipeg, Manitoba. R3B 1Y6 Tel: 204-984-7166 Fax:204-984-6978 email:saro.bascaramurty@nrc-cnrc.gc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Thu Jun 30 04:57:58 2011 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Jun 30 04:58:01 2011 Subject: [Histonet] Could you please recommend? Cryostats. In-Reply-To: References: Message-ID: You can't get any better than a Leica 3050. That is top of the line to me. Unless there's some really expensive brand out there I'm not aware of. I love our 3050. I guess your problem is you need a UV lamp, and the 3050 doesn't have one. I would say stick with Leica, their cryostats are awesome. Expensive, but awesome. Their newest model has a UV light and is smaller than the 3050, http://bit.ly/isrHB2 No, I don't work for Leica, but I've always used their cryostats and I've always sectioned beautifully with them. Emily ps Our Leica representative is Scottish, so I enjoy hearing his accent. Does anyone else have Nick Grahame as a Leica rep? There have been times when I've asked him to repeat what he said because his accent was so thick. Don't get me wrong, I love it! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron From BMolinari <@t> heart.thi.tmc.edu Thu Jun 30 06:09:31 2011 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Jun 30 06:09:24 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: References: <498DDA84FDA449B284C69014FBF60684@JoannePC> <9A9AE8ABCC77491891AA5A5A33A5967C@DJ4VDH31> Message-ID: I once went to an interview at a research lab , will remain unnamed, and they asked if I could cut 300 /hr. I said "Is this a trick question?" They just stared back. I withdrew my app. LOL Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, June 28, 2011 4:27 PM To: 'Markus F. Meyenhofer'; Stella Mireles; Joanne Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per Perhaps your med techs should try cutting 100 an hour with quality sections that a pathologist can read!!! They are to accustom to high throughput testing with only pushing a button in some cases. I know med tech that are excellent Histologist but they do the work too. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Markus F. Meyenhofer Sent: Tuesday, June 28, 2011 4:22 PM To: Stella Mireles; Joanne Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per Maybe 100 sections from one block??? ----- Original Message ----- From: "Stella Mireles" To: "Joanne" Cc: Sent: Tuesday, June 28, 2011 4:49 PM Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per > Joanne is not alone. > > The lab I work at has informed us that due to their own research, a single > histotech should be able to do 100 blocks an hour. > I'm not sure if they expect embedding, trimming, writing slides, > sectioning > and manual staining in this goal. > > I am a seasoned histotech, and have tried to speak to my lab manager and > lab > supervisor, (both are med. techs and have very little insight into what is > involved in producing a high quality slide), but their goal remains the > same. > > I have an idea: I feel like printing some of your responses to Joanne > question and showing them that their goal is unattainable as well. > > Any other suggestions. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Thu Jun 30 06:12:14 2011 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Jun 30 06:12:07 2011 Subject: [Histonet] How many tissues an histo tech is suppose to cut per In-Reply-To: References: <498DDA84FDA449B284C69014FBF60684@JoannePC> <9A9AE8ABCC77491891AA5A5A33A5967C@DJ4VDH31> Message-ID: Sorry....I meant 300 in a 8hr day..including embedding, etc.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, June 30, 2011 6:10 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per I once went to an interview at a research lab , will remain unnamed, and they asked if I could cut 300 /hr. I said "Is this a trick question?" They just stared back. I withdrew my app. LOL Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, June 28, 2011 4:27 PM To: 'Markus F. Meyenhofer'; Stella Mireles; Joanne Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per Perhaps your med techs should try cutting 100 an hour with quality sections that a pathologist can read!!! They are to accustom to high throughput testing with only pushing a button in some cases. I know med tech that are excellent Histologist but they do the work too. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Markus F. Meyenhofer Sent: Tuesday, June 28, 2011 4:22 PM To: Stella Mireles; Joanne Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per Maybe 100 sections from one block??? ----- Original Message ----- From: "Stella Mireles" To: "Joanne" Cc: Sent: Tuesday, June 28, 2011 4:49 PM Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per > Joanne is not alone. > > The lab I work at has informed us that due to their own research, a single > histotech should be able to do 100 blocks an hour. > I'm not sure if they expect embedding, trimming, writing slides, > sectioning > and manual staining in this goal. > > I am a seasoned histotech, and have tried to speak to my lab manager and > lab > supervisor, (both are med. techs and have very little insight into what is > involved in producing a high quality slide), but their goal remains the > same. > > I have an idea: I feel like printing some of your responses to Joanne > question and showing them that their goal is unattainable as well. > > Any other suggestions. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aarondan <@t> telus.net Thu Jun 30 08:59:16 2011 From: aarondan <@t> telus.net (aarondan@telus.net) Date: Thu Jun 30 08:59:20 2011 Subject: [Histonet] Grossing benchmarks Message-ID: <30425816.1415800.1309442356267.JavaMail.nitido@priv-edtnes91> Histonetters, I need help finding a reference for the amount of specimens/cases a PA should be able to gross in a day. We use the L4E system and have approxamately 10% Level 5 cases and 10% Level 6 cases. Also, have you found any references for the productivity benchmarks of a Histo lab that performs gross, embedding, cutting, frozen sections, and special stains but not cytology or IHC? Thanks!! Danielle- PA/TechIII BSc/MLS From rjbuesa <@t> yahoo.com Thu Jun 30 09:51:50 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 30 09:51:53 2011 Subject: [Histonet] Grossing benchmarks In-Reply-To: <30425816.1415800.1309442356267.JavaMail.nitido@priv-edtnes91> Message-ID: <1309445510.32770.YahooMailClassic@web65704.mail.ac4.yahoo.com> Read the attachments for the information you have requested. Ren? J. --- On Thu, 6/30/11, aarondan@telus.net wrote: From: aarondan@telus.net Subject: [Histonet] Grossing benchmarks To: histonet@lists.utsouthwestern.edu Date: Thursday, June 30, 2011, 9:59 AM ???Histonetters, ???I need help finding a reference for the amount of specimens/cases a PA ???should? be? able? to? gross? in a day.? We use the L4E system and have ???approxamately 10% Level 5 cases and 10% Level 6 cases. ???Also, have you found any references for the productivity benchmarks of ???a? Histo lab that performs gross, embedding, cutting, frozen sections, ???and special stains but not cytology or IHC? ???Thanks!! ???Danielle- PA/TechIII BSc/MLS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Thu Jun 30 10:55:08 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Thu Jun 30 10:55:13 2011 Subject: [Histonet] Luciferase Message-ID: Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From rjbuesa <@t> yahoo.com Thu Jun 30 11:00:51 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 30 11:01:00 2011 Subject: [Histonet] Luciferase In-Reply-To: Message-ID: <1309449651.4032.YahooMailClassic@web65709.mail.ac4.yahoo.com> You would have to have an antibody?to it?and I don't know any one?exists, but I can be wrong. Ren? J. --- On Thu, 6/30/11, sgoebel@mirnarx.com wrote: From: sgoebel@mirnarx.com Subject: [Histonet] Luciferase To: histonet@lists.utsouthwestern.edu Date: Thursday, June 30, 2011, 11:55 AM Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Jun 30 11:03:13 2011 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Jun 30 11:03:17 2011 Subject: [Histonet] RE: Luciferase In-Reply-To: Message-ID: <14E2C6176416974295479C64A11CB9AE0646F730@SBS2K8.premierlab.local> You can, we have done this in the past. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Thursday, June 30, 2011 9:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Luciferase Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu Jun 30 11:13:03 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Jun 30 11:13:16 2011 Subject: [Histonet] Luciferase In-Reply-To: <1309449651.4032.YahooMailClassic@web65709.mail.ac4.yahoo.com> References: <1309449651.4032.YahooMailClassic@web65709.mail.ac4.yahoo.com> Message-ID: <4E0C684E.2B7F.00C9.1@geisinger.edu> ok there is a really BAD religious joke coming soon, isn't there? >>> Rene J Buesa 6/30/2011 12:00 PM >>> You would have to have an antibody to it and I don't know any one exists, but I can be wrong. Ren? J. --- On Thu, 6/30/11, sgoebel@mirnarx.com wrote: From: sgoebel@mirnarx.com Subject: [Histonet] Luciferase To: histonet@lists.utsouthwestern.edu Date: Thursday, June 30, 2011, 11:55 AM Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From jqb7 <@t> cdc.gov Thu Jun 30 11:14:08 2011 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Thu Jun 30 11:14:12 2011 Subject: [Histonet] Luciferase In-Reply-To: <4E0C684E.2B7F.00C9.1@geisinger.edu> References: <1309449651.4032.YahooMailClassic@web65709.mail.ac4.yahoo.com> <4E0C684E.2B7F.00C9.1@geisinger.edu> Message-ID: We can only hope, LOL! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, June 30, 2011 12:13 PM To: histonet@lists.utsouthwestern.edu; sgoebel@mirnarx.com; Rene J Buesa Subject: Re: [Histonet] Luciferase ok there is a really BAD religious joke coming soon, isn't there? >>> Rene J Buesa 6/30/2011 12:00 PM >>> You would have to have an antibody to it and I don't know any one exists, but I can be wrong. Ren? J. --- On Thu, 6/30/11, sgoebel@mirnarx.com wrote: From: sgoebel@mirnarx.com Subject: [Histonet] Luciferase To: histonet@lists.utsouthwestern.edu Date: Thursday, June 30, 2011, 11:55 AM Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Thu Jun 30 11:17:47 2011 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Thu Jun 30 11:17:50 2011 Subject: [Histonet] Benchmark Information Message-ID: <1872B4A455B7974391609AD8034C79FC026DFE1F@LBEXCH01.hchd.local> Hello to all in histoland. I am currently trying to find a hospital that is comparable to ours for benchmark info. We a 260 bed county hospital and we are currently processing 10,000 plus cases and have 4 techs,1 supervisor and 1 assistant. If there is anyone comparable to us I would like to have info concerning how many techs, assistants, and how many cases, block and slides that were done last year and unp until now. We are currently trying to justify another tech position. Any help would ne greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From billodonnell <@t> catholichealth.net Thu Jun 30 11:18:14 2011 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Jun 30 11:18:27 2011 Subject: [Histonet] Luciferase (OK here it is) In-Reply-To: <4E0C684E.2B7F.00C9.1@geisinger.edu> References: <1309449651.4032.YahooMailClassic@web65709.mail.ac4.yahoo.com> <4E0C684E.2B7F.00C9.1@geisinger.edu> Message-ID: <4940DF6D1C5FDF48931B6966AAEF93950D32EC@chimsx08.CHI.catholichealth.net> OK here it isYou can stainfor it, but my policy is to only do it if "If the devil makes me do it" (Flip Wilson) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, June 30, 2011 11:13 AM To: histonet@lists.utsouthwestern.edu; sgoebel@mirnarx.com; Rene J Buesa Subject: Re: [Histonet] Luciferase ok there is a really BAD religious joke coming soon, isn't there? >>> Rene J Buesa 6/30/2011 12:00 PM >>> You would have to have an antibody to it and I don't know any one exists, but I can be wrong. Ren? J. --- On Thu, 6/30/11, sgoebel@mirnarx.com wrote: From: sgoebel@mirnarx.com Subject: [Histonet] Luciferase To: histonet@lists.utsouthwestern.edu Date: Thursday, June 30, 2011, 11:55 AM Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sgoebel <@t> mirnarx.com Thu Jun 30 11:18:29 2011 From: sgoebel <@t> mirnarx.com (sgoebel@mirnarx.com) Date: Thu Jun 30 11:18:33 2011 Subject: [Histonet] Luciferase In-Reply-To: <4E0C684E.2B7F.00C9.1@geisinger.edu> References: <1309449651.4032.YahooMailClassic@web65709.mail.ac4.yahoo.com> <4E0C684E.2B7F.00C9.1@geisinger.edu> Message-ID: Someone just responded ?only if it?s possessed? I laughed =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: Thursday, June 30, 2011 11:13 AM To: histonet@lists.utsouthwestern.edu; Sarah Goebel; Rene J Buesa Subject: Re: [Histonet] Luciferase ok there is a really BAD religious joke coming soon, isn't there? >>> Rene J Buesa 6/30/2011 12:00 PM >>> You would have to have an antibody to it and I don't know any one exists, but I can be wrong. Ren? J. --- On Thu, 6/30/11, sgoebel@mirnarx.com wrote: From: sgoebel@mirnarx.com Subject: [Histonet] Luciferase To: histonet@lists.utsouthwestern.edu Date: Thursday, June 30, 2011, 11:55 AM Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Tom_Wells <@t> bcit.ca Thu Jun 30 11:23:34 2011 From: Tom_Wells <@t> bcit.ca (Tom Wells) Date: Thu Jun 30 11:23:48 2011 Subject: [Histonet] Luciferase Message-ID: Question: Can you stain Luciferase in tissues? Answer: Yes, but it's devilishly hard to do. Sorry, I couldn't help myself. Tom Tom Wells BSc, ART Faculty School of Medical Laboratory Sciences British Columbia Institute of Technology Burnaby, BC Canada Phone: 604-412-7594 Cell: 778-228-4102 From rjbuesa <@t> yahoo.com Thu Jun 30 11:26:15 2011 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 30 11:26:19 2011 Subject: [Histonet] Benchmark Information In-Reply-To: <1872B4A455B7974391609AD8034C79FC026DFE1F@LBEXCH01.hchd.local> Message-ID: <1309451175.58512.YahooMailClassic@web65703.mail.ac4.yahoo.com> According with my experience your complement is adequate for your workload and if I were the one you have to convince to get an additional HT,?I would?give you a very?hard time. Ren? J. --- On Thu, 6/30/11, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Benchmark Information To: histonet@lists.utsouthwestern.edu Date: Thursday, June 30, 2011, 12:17 PM Hello to all in histoland.? I am currently trying to find a hospital that is comparable to ours for benchmark info.? We a 260 bed county hospital and we are currently processing 10,000 plus cases and have 4 techs,1 supervisor and 1 assistant.? If there is anyone comparable to us I would like to have info concerning how many techs, assistants, and how many cases, block and slides that were done last? year and unp until now.? We are currently trying to justify another tech position.? Any help would ne greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jameshart77 <@t> gmail.com Thu Jun 30 11:26:54 2011 From: jameshart77 <@t> gmail.com (James Hart) Date: Thu Jun 30 11:26:58 2011 Subject: [Histonet] Luciferase In-Reply-To: References: Message-ID: Question: Can you stain Luciferase in tissues? Answer: Yes, but the background noise is hell! On Thu, Jun 30, 2011 at 12:23 PM, Tom Wells wrote: > > Question: Can you stain Luciferase in tissues? > Answer: Yes, but it's devilishly hard to do. > > Sorry, I couldn't help myself. > Tom > > Tom Wells BSc, ART > Faculty > School of Medical Laboratory Sciences > British Columbia Institute of Technology > Burnaby, BC > Canada > Phone: 604-412-7594 > Cell: 778-228-4102 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From cbarone <@t> NEMOURS.ORG Thu Jun 30 11:36:52 2011 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Thu Jun 30 11:37:14 2011 Subject: [Histonet] Cutiing of dextran beads (snap frozen) Message-ID: Histonetters....We have received some dextran beads (coated with cells) that are "snap frozen"...for cutting. We have done these beads before in histogel/paraffin...but are curious as to how best to handle them snap frozen. We have a nice bead with no cells on our first to snap frozen sample blocks....Help? Is it the temperature? We cut at -25 on average. From trathborne <@t> somerset-healthcare.com Thu Jun 30 11:36:25 2011 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Jun 30 11:38:22 2011 Subject: [Histonet] Benchmark Information In-Reply-To: <1309451175.58512.YahooMailClassic@web65703.mail.ac4.yahoo.com> References: <1872B4A455B7974391609AD8034C79FC026DFE1F@LBEXCH01.hchd.local> <1309451175.58512.YahooMailClassic@web65703.mail.ac4.yahoo.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB7570702E7C7@SMCMAIL01.somerset-healthcare.com> You might want to ask for a per diem position, justifying it for use during vacations. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 30, 2011 12:26 PM To: histonet@lists.utsouthwestern.edu; Allison DScott Subject: Re: [Histonet] Benchmark Information According with my experience your complement is adequate for your workload and if I were the one you have to convince to get an additional HT,?I would?give you a very?hard time. Ren? J. --- On Thu, 6/30/11, Scott, Allison D wrote: From: Scott, Allison D Subject: [Histonet] Benchmark Information To: histonet@lists.utsouthwestern.edu Date: Thursday, June 30, 2011, 12:17 PM Hello to all in histoland.? I am currently trying to find a hospital that is comparable to ours for benchmark info.? We a 260 bed county hospital and we are currently processing 10,000 plus cases and have 4 techs,1 supervisor and 1 assistant.? If there is anyone comparable to us I would like to have info concerning how many techs, assistants, and how many cases, block and slides that were done last? year and unp until now.? We are currently trying to justify another tech position.? Any help would ne greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From mcauliff <@t> umdnj.edu Thu Jun 30 11:42:08 2011 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jun 30 11:38:34 2011 Subject: [Histonet] Luciferase In-Reply-To: <4E0C684E.2B7F.00C9.1@geisinger.edu> References: <1309449651.4032.YahooMailClassic@web65709.mail.ac4.yahoo.com> <4E0C684E.2B7F.00C9.1@geisinger.edu> Message-ID: <4E0CA760.6010008@umdnj.edu> It is a devil to stain. Geoff On 6/30/2011 12:13 PM, Angela Bitting wrote: > ok there is a really BAD religious joke coming soon, isn't there? > > >>>> Rene J Buesa 6/30/2011 12:00 PM>>> > You would have to have an antibody to it and I don't know any one > exists, but I can be wrong. > Ren? J. > > --- On Thu, 6/30/11, sgoebel@mirnarx.com wrote: > > > From: sgoebel@mirnarx.com > Subject: [Histonet] Luciferase > To: histonet@lists.utsouthwestern.edu > Date: Thursday, June 30, 2011, 11:55 AM > > > Can you stain for luciferase in tissue? > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Mandy.Bell <@t> chomp.org Thu Jun 30 11:52:13 2011 From: Mandy.Bell <@t> chomp.org (Bell, Mandy) Date: Thu Jun 30 11:52:33 2011 Subject: [Histonet] blade donations Message-ID: I just wanted to thank everyone for the overwhelming response we got for microtome blade donations. I think that we are all set now. Thank you so much for your help. Here is a link to a youtube clip of the hospital where our pathologist volunteers if anyone is interested: www.youtube.com/watch?v=lb3TMtzZCXU Thanks again, Mandy M Bell , HTL (ASCP) Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail From rsrichmond <@t> gmail.com Thu Jun 30 12:14:39 2011 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Jun 30 12:14:44 2011 Subject: [Histonet] Re: Could you please recommend? Cryostats Message-ID: Amos Brooks observes: >>I have used cryostats clinically as well as in research, and one thing I noticed absent from most cryostats is the ability to adjust your block. For the life of me I cannot conceive of a reason why a company would have the audacity to sell a cryostat that does not allow the block to tilt. If I was in the market for a cryostat, I would not even consider one without this feature.<< The cryostat (microtome in a freezer) was introduced into surgical pathology around 1960, when it fairly rapidly replaced the old wet-knife (e.g. Sartorius) microtome. By 1964, when I was a pathology intern, they had come into fairly widespread use, and were in almost universal use before 1980. The early International (Damon/IEC) cryostats indeed had a very large black knob that enabled you to tilt the block. The beginner invariably grabbed that knob and twisted it, putting the block at a weird tilt. Usually before you knew it you were cutting metal, a serious problem back in the days of sharpening microtome blades. When you're doing a surgical frozen section, you have no time for such gyrations. That's probably why this bug/undocumented feature was eliminated. But man, those early Internationals were versatile - the things you could do with them in a research lab were limited only by your imagination and technical expertise. I think with six months lead time you could have one of those things running underwater. Bob Richmond Samurai Pathologist Knoxville TN From jonesly <@t> mir.wustl.edu Thu Jun 30 12:18:00 2011 From: jonesly <@t> mir.wustl.edu (Jones, Lynne) Date: Thu Jun 30 12:18:06 2011 Subject: [Histonet] UV decontamination of cryostats in the US In-Reply-To: <499ee661-9cd6-432d-8ba2-637ea25c042b@rad-vmsrvedge1.rad.wustl.edu> References: <499ee661-9cd6-432d-8ba2-637ea25c042b@rad-vmsrvedge1.rad.wustl.edu> Message-ID: <208330218E165F45AB6307B787F5EA47016BA86DFD6E@RAD-VMSRVEXV2.rad.wustl.edu> Hello - I've been following discussion thread regarding a suitable cryostat for use with human specimens. Like Saro (who is in Canada), we also work in a research setting, but primarily with rodent tissues, and only section human tissues intermittently. (We may go for weeks without using our lovely little Microm at all.) The Institutional Biosafety Committee and Environmental Health and Safety require that we have documentation of training for all cryostat users and a written SOP for disinfection after we work with potentially infections materials. When I was looking for options that would not require defrosting the instrument, I learned that the cryostat disinfectant sold outside the US for use at working temperatures (i.e., below freezing), has proven ineffective and is therefore not EPA registered. It also seems like UV lights are just window dressing, since UV is ineffective on soiled areas (or anyplace the light doesn't reach). My understanding is that US regs require that instruments be defrosted and cleaned before disinfection (on an established schedule) with a EPA registered disinfectant. Right now, our SOP is to fully defrost, clean and chemically disinfect the instrument then replace the blade after every time we section human tissue (unless it is clearly posted that the cryostat has been reserved for human tissue use). Lining the chamber with foil makes cleanup easier, but it is still a hassle. Are there other/easier alternatives? Thanks, Lynne Jones Lab Manager Mach Research Group Radiological Chemistry Washington University School of Medicine St. Louis, MO -----Original Message----- (snip) ---------------------------------------------------------------------- Message: 7 Date: Wed, 29 Jun 2011 15:23:11 -0400 (EDT) From: "Nicole Tatum" Subject: Re: [Histonet] Could you please recommend? (Bascaramurty, Saro) To: "histotech@imagesbyhopper.com" , histonet@lists.utsouthwestern.edu Message-ID: <1794.208.62.167.196.1309375391.squirrel@webmail.realpages.com> Content-Type: text/plain;charset=iso-8859-1 Not sure about the UV thingy but I love the Leica 1850 or the 1510 works great. But, if I had to choose between the two I would go with the Leica 1850.. You can purchased a used cryo for about 12-15,000. Most times with a one year warrenty. I recommend calling Margaret at IMEB for a quote. She is wonderful and will find what you need. Nicole Tatum HT(ASCP) I second the Leica! :o) > > Michelle > > Sent from my iPhone > > On Jun 29, 2011, at 1:14 PM, "White, Lisa M." wrote: > >> We use the Leica CM1850 UV. It is easy to use for sectioning as well as >> UV decontamination. Pricing fell in line with other vendors. I have >> used Shandon, Leitz, Leica and a unit so old I think it came with Noah >> on the boat don't even remember the maker. Leica is the favorite. It >> sections well and was the first one that the anti-roll plate would work >> correctly. It decontaminates well. No regrets, love it and would >> recommend. >> >> >> >> Lisa White, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > On Wed, Jun 29, 2011 at 1:02 PM, wrote: > Message: 8 > Date: Wed, 29 Jun 2011 12:57:05 -0400 > From: "Bascaramurty, Saro" > Subject: [Histonet] Could you please recommend? > To: 'Histonet' > > Hi, > > I am in a research lab and currently we are looking into buying a good > cryostat to work with human samples. I have always used a Leica model and > currently we own a Leica 3050S. We would like get one that comes with > decontamination feature (UV lamp etc...). I would like to know your positive > and negative experiences with the models that you own. Which ones we should > stay away from and which ones that you did not regret that you purchased it. > Also some pricing details would be helpful. > > There were some earlier postings on this topic earlier which I did not > bother to save. > > Thank you all in advance for your anticipated feedback. > > > Saro Bascaramurty > > Technical Officer > Institute for Biodiagnostics > National Research Council > 435 Ellice Avenue, > Winnipeg, Manitoba. R3B 1Y6 > > Tel: 204-984-7166 > Fax:204-984-6978 > email:saro.bascaramurty@nrc-cnrc.gc.ca > ------------------------------ Message: 14 Date: Thu, 30 Jun 2011 05:57:58 -0400 From: Emily Sours Subject: Re: [Histonet] Could you please recommend? Cryostats. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 You can't get any better than a Leica 3050. That is top of the line to me. Unless there's some really expensive brand out there I'm not aware of. I love our 3050. I guess your problem is you need a UV lamp, and the 3050 doesn't have one. I would say stick with Leica, their cryostats are awesome. Expensive, but awesome. Their newest model has a UV light and is smaller than the 3050, http://bit.ly/isrHB2 No, I don't work for Leica, but I've always used their cryostats and I've always sectioned beautifully with them. Emily ps Our Leica representative is Scottish, so I enjoy hearing his accent. Does anyone else have Nick Grahame as a Leica rep? There have been times when I've asked him to repeat what he said because his accent was so thick. Don't get me wrong, I love it! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron ------------------------------ The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. From TMcNemar <@t> lmhealth.org Thu Jun 30 13:06:30 2011 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Jun 30 13:06:40 2011 Subject: [Histonet] p16 Protocol.... Message-ID: Hello all, I was wondering if anyone would be willing to share a protocol for p16 (Biogenix pre-dilute) antibody on the Benchmark XT. I'm having a little trouble getting anything to stain. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From akbitting <@t> geisinger.edu Thu Jun 30 13:50:12 2011 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Jun 30 13:50:23 2011 Subject: [Histonet] Luciferase In-Reply-To: <4E0CA760.6010008@umdnj.edu> References: <1309449651.4032.YahooMailClassic@web65709.mail.ac4.yahoo.com> <4E0C684E.2B7F.00C9.1@geisinger.edu> <4E0CA760.6010008@umdnj.edu> Message-ID: <4E0C8D23.2B7F.00C9.1@geisinger.edu> You can, but the retrieval involves a young priest and an old priest. ff McAuliffe 6/30/2011 12:42 PM >>> It is a devil to stain. Geoff On 6/30/2011 12:13 PM, Angela Bitting wrote: > ok there is a really BAD religious joke coming soon, isn't there? > > >>>> Rene J Buesa 6/30/2011 12:00 PM>>> > You would have to have an antibody to it and I don't know any one > exists, but I can be wrong. > Ren? J. > > --- On Thu, 6/30/11, sgoebel@mirnarx.com wrote: > > > From: sgoebel@mirnarx.com > Subject: [Histonet] Luciferase > To: histonet@lists.utsouthwestern.edu > Date: Thursday, June 30, 2011, 11:55 AM > > > Can you stain for luciferase in tissue? > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Thu Jun 30 14:02:08 2011 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Jun 30 14:02:12 2011 Subject: [Histonet] Luciferase In-Reply-To: References: Message-ID: <9FE33752FA3F3647BC85BCDC3EA6C3D701019235@usctmx1176.merck.com> All kidding aside, assuming your talking about something tagged with firefly luciferase you can try using monoclonal MA1-16880 from Thermo Fisher. Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sgoebel@mirnarx.com Sent: Thursday, June 30, 2011 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Luciferase Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From bruski53 <@t> mac.com Thu Jun 30 18:05:35 2011 From: bruski53 <@t> mac.com (Bruce Gapinski) Date: Thu Jun 30 18:06:54 2011 Subject: [Histonet] "grossing" again... Message-ID: <4DADB7EA-3906-41DA-9AE2-620494E94D9E@mac.com> Is anyone anticipating new CAP changes this year in their lab with respect to "grossing" tissue. We are not in the same site as the doctors and we've been submitting specific surgical specimens for the Pathologist for 20 years. GI bxs, prostate needle bxs, etc. We've also been grossing dermatology specimens since 1970. Cutting skin ellipses, submitting punch bxs, and on and on. All off site. Our Pathologist thinks it is more technical to embed the punch bx, than to place it in a cassette. Is it true, we can't gross anything with just an HT certificate? How about if we use cameras to watch our grossing from the hospital? Your thoughts please Respectfully, Bruce Gapinski HT (ASCP)